LOCUS       DASUIV010000001         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_594, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000001 DASUIV010000000
VERSION     DASUIV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /gene="argS"
                     /locus_tag="VFW59_00005"
     CDS             <1..1056
                     /gene="argS"
                     /locus_tag="VFW59_00005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5362629.1"
                     /translation="ARSYMAKETVEADDQHITGSGDVNDAEAIRHFAVAYLRREQDLD
                     LRAFEVEFDVFSLESALYTEGKVEEAVSKLIASGHTYEQDGALWLRTTDFGDDKDRVM
                     RKTDGGYTYFVPDVAYHLEKWKRGFVRVINEQGSDHHSTITRVRAGLQALDVGIPKGW
                     PDYVLHQMVTVLKNGEEVKISKRAGSYVTLRDLIDEVGCDATRYFLAARHPDSQLVFD
                     IDLAKSQSNDNPVYYIQYAHARIHAVLAQWGGDRQSLHRADVSALGSDYETQLLQKMI
                     DFPEVIERAAEELAPHLVAFYLKDLAAEFHSYYNASRFLVDDEQVKLARLALIEALAQ
                     VLKNGLALLGVSAPEQM"
     gene            1077..1685
                     /locus_tag="VFW59_00010"
     CDS             1077..1685
                     /locus_tag="VFW59_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5362630.1"
                     /translation="MSRNPGSKAAPRKGGSSLMTGILVGMIIGVGLAAGLAWFIMKSP
                     SPFVAKEQAVAKAVPPVKQQAPVAAPAVASVPTPASAVASDKPRFEFYKVLTDKQDAS
                     VAPDKPKTEAKPKAAEVKPQPDGGPLLLQAGSFSNEGDAEKLKARIAFMGAEANIQTV
                     SIPDKGVWHRVRLGPYKNADDMNRVRNMLKQNGVDATPIHAQ"
     gene            1711..2331
                     /locus_tag="VFW59_00015"
     CDS             1711..2331
                     /locus_tag="VFW59_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX5362631.1"
                     /translation="MRSIIKSLAIVAALLFGGVACAAEAGKDYKLLNAVQPTSTKKIE
                     VLEFFFYGCPHCFHLHRDLTAWSKSLPKDVELTYVPVVFRDSWEPMAWTFYALDALGQ
                     REQLHDALFQAWNVSNVDLSDEAKILNYLAPRGVDRTKFSAAYNSFAVQSKVTRAKQM
                     IRSYGISGTPTIVVEGKYSITGLQPADSIRVLNEVIAKVRKERSKH"
     gene            2368..3231
                     /locus_tag="VFW59_00020"
     CDS             2368..3231
                     /locus_tag="VFW59_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5362632.1"
                     /translation="MKDSNAGLSQSSTLDPQSVVISGASSGIGLALARHYLERGATVA
                     ACARRGEPLQTLAAEFPGKVYCYALDVRDAAALREAAADFIARAGVPDIVIANAGVSR
                     GTLTEYAEDNDAFRQVMDINVLGMVQTFQPFIAPMRSAADNRPSFASSPACGRGGKRE
                     KHFTLVGIASVAGFRGLPGAGAYSASKAAAISYLESLRVELRGSGVRVVTICPGYIKT
                     PMTAVNPYPMPFILEVNEAARRMAGVIERGCSFAVIPWQMGLVGRLLKLLPNWLYDLL
                     LKKAPYKPRNF"
     gene            3428..>3555
                     /locus_tag="VFW59_00025"
     CDS             3428..>3555
                     /locus_tag="VFW59_00025"
                     /inference="COORDINATES: protein motif:HMM:NF021885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit N-terminal domain-containing protein"
                     /protein_id="HEX5362633.1"
                     /translation="MGKETVSDGDRRNFLIKLSSVVGGAGVAATCVPFVASMNPSS"
BASE COUNT          728 a         1014 c         1134 g          679 t
ORIGIN      
        1 gcgcgcagct acatggcgaa agagaccgtc gaggccgacg accagcacat cacaggctca
       61 ggcgacgtga acgatgccga ggcgatccgc catttcgcgg tggcctatct gcgccgcgaa
      121 caggatctgg atctgcgcgc cttcgaggtg gagttcgacg tattctcgct ggagtccgcg
      181 ctgtataccg agggcaaggt ggaggaggcg gtcagcaagc tgatcgccag cggccacact
      241 tacgagcagg acggtgcgct gtggctgcgc accaccgatt tcggcgacga caaagaccgc
      301 gtgatgcgca agaccgacgg cggctacacc tacttcgtgc cggacgtggc ctaccatctg
      361 gaaaaatgga agcgcggctt cgtgcgcgtc atcaacgaac agggttccga tcaccactcc
      421 acgattacaa gggtgcgcgc cggcctgcag gcgctggacg tcggcatccc caagggctgg
      481 ccggactacg tgctgcacca gatggtgacg gtgctgaaga acggagaaga ggtgaagata
      541 tccaagcgcg cgggcagcta tgtcacgctg cgcgacctga tcgacgaggt gggctgcgat
      601 gccacgcgct atttcctcgc cgcgcgtcat cccgattcgc agctggtgtt cgacatcgat
      661 ctggccaagt cgcagagcaa cgacaacccc gtctattaca tccagtacgc ccatgcccgc
      721 attcatgcgg tgctggcgca gtggggcggc gaccgtcaga gcctgcatcg ggccgacgtc
      781 agcgcgctgg gcagcgacta cgaaacgcag ttgctgcaga agatgatcga cttccccgag
      841 gtgatcgagc gggctgcgga agagctggca ccgcatctgg tggcgttcta cctcaaggat
      901 ttggccgccg aatttcacag ctactataat gcctcgcgct tcctggtcga cgacgagcag
      961 gtcaagctgg cgcgtctggc gctgatcgag gcgctggcgc aggtattgaa gaatggtctg
     1021 gcactgctcg gggtgagcgc gccggaacag atgtaatgca caggaataga caagacatga
     1081 gcagaaaccc aggtagcaaa gccgcgccac gcaagggcgg cagttcgttg atgaccggca
     1141 ttctggtcgg catgatcatc ggtgtcggcc tggcggccgg actggcctgg ttcatcatga
     1201 agtcgccgag cccgttcgtg gcgaaggagc aggcggtagc caaggccgtg cctccggtca
     1261 aacagcaggc acccgtcgcc gcacccgccg tagccagtgt gccgacgccg gcttccgccg
     1321 tggcatcgga taagccccgt ttcgagttct acaaggtgct gaccgacaaa caggacgcga
     1381 gcgttgcgcc ggacaagcca aaaacagagg ccaagcccaa ggcggcggaa gtcaagccgc
     1441 agccggacgg cgggccgttg ctgttgcagg cgggttcctt ctccaatgag ggggatgcgg
     1501 agaagctcaa ggccagaatc gccttcatgg gcgccgaggc caacatccag accgtgagca
     1561 tcccggacaa gggcgtgtgg catcgggtgc gtctggggcc atacaaaaat gccgacgata
     1621 tgaaccgcgt gcgcaatatg ttgaaacaaa atggggtgga tgccactcca atccacgcgc
     1681 aataagcgtg tccatcacaa ggagcgataa atgagaagca tcatcaagag tctggcgatc
     1741 gtggcggccc tgttgtttgg cggcgtggct tgtgctgccg aggcgggcaa ggactacaaa
     1801 ctgctgaatg ccgtacagcc gaccagcacc aagaaaatcg aagtgctgga gtttttcttc
     1861 tatggctgtc cgcattgctt ccatctgcac cgcgatctga cggcatggag caagagcctg
     1921 cccaaggacg tcgagttgac ctatgtgccg gtggtcttcc gcgattcatg ggagccgatg
     1981 gcatggacct tctatgcgct ggatgcgctg ggccagcgtg agcagctgca cgatgcgctg
     2041 tttcaggcat ggaacgtcag caatgtcgac ctgagcgatg aggccaaaat cttgaactac
     2101 ctcgcgccgc gcggcgtgga tcgcaccaag ttctcggcgg cctataattc ctttgcggta
     2161 cagagcaagg tgacgcgcgc caagcagatg atccgcagct acggtatcag cggcacaccg
     2221 accatcgtgg tcgagggcaa gtattccatc accggtctgc agcccgccga cagcatccgc
     2281 gtgttgaatg aagtgatcgc caaagtccgc aaggaacgca gcaaacacta gctaggattt
     2341 aggactgagg atttaggatt gagtagggtg aaagacagca acgcagggtt gtctcaatcc
     2401 tcaaccctcg atcctcaatc cgtcgttatt agcggcgcgt cgagcggcat cggtcttgcc
     2461 ctcgcgcgcc attatcttga gcgcggtgcg accgtcgcgg cttgcgcccg gcgcggcgag
     2521 ccgctgcaaa ccctcgcggc agaatttccc ggaaaagttt attgctacgc gctggacgtg
     2581 cgcgacgcgg cagccttgcg cgaggcggcg gcggatttca tcgctcgcgc cggtgtgccg
     2641 gatatcgtga tcgccaacgc gggtgtgagt cgcggcacgc tgactgaata cgccgaagac
     2701 aacgatgcat tccggcaggt gatggatatc aacgtgctgg gcatggtgca gacctttcag
     2761 ccgttcattg cgccgatgcg ctcggcagcc gacaaccggc cctcgtttgc ctcctctccc
     2821 gcttgcggga gaggaggcaa acgagaaaaa cattttaccc ttgtaggtat agccagcgtg
     2881 gcgggttttc gcgggctgcc cggtgcgggt gcgtattcgg cctccaaggc ggcggcgatc
     2941 tcctatctgg agtcgttgcg cgtcgagttg cgtggcagcg gcgtgcgtgt ggtgacgatc
     3001 tgtcccggtt atatcaagac gccgatgacc gcagtgaatc cttacccgat gccgttcatt
     3061 ctcgaggtca acgaggctgc gcggcgtatg gccggagtca tcgagcgcgg ttgttcgttc
     3121 gcggtgattc cgtggcagat ggggctggtc ggacgcctgt tgaagttgct gccaaactgg
     3181 ctgtatgacc tgctgttgaa gaaggcgccc tataagccgc gcaatttctg acagtatgcg
     3241 cggtgccttg caccctgcat cagcggtaac ctatcccctc atccctcggg gttactccca
     3301 aagtgcaacc ctgtaatcct ttggtttagt ttccaaccca cccttcatat ccattgtgag
     3361 ttaaggcatt acaggcgtag catgccccgc tgtatcagaa aacggtaata ttttcggggg
     3421 cgtgaagatg gggaaagaaa cggtaagcga tggcgatcgg cgcaactttc tgatcaagct
     3481 gtcttctgtg gtgggcggag cgggggtggc ggcgacatgc gtgcctttcg tcgccagtat
     3541 gaatcccagc tccga
//
LOCUS       DASUIV010000002         5345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1023126, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000002 DASUIV010000000
VERSION     DASUIV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023126"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFW59_00030"
     CDS             complement(<1..189)
                     /locus_tag="VFW59_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5362634.1"
                     /translation="MKATTPKDSAGTGPLNFQRPLATVDVAIFTIISDRLHVLLVQRN
                     NDPGEPFPNKWALAGGFVD"
     gene            298..1317
                     /locus_tag="VFW59_00035"
     CDS             298..1317
                     /locus_tag="VFW59_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5362635.1"
                     /translation="MFGIRFIKAEPTTYLMAFRGGKIVKEGAGLSLLYFGPTTSLVAV
                     PVGSRALDFIFQPVTSDFQSVTVQGQVSYRVSEPRKLAGMLDYSLRSDGRYASDDPEK
                     LRDRVLGVAEVLIQKNLKKMDLKQALLASDEIALVIEDGLRGQAEIQSLGIDVLRASV
                     LAIKPTPETAKALEAEARESILRRADEAVFSRRNAAVEQERAIRESELDTEVAVEQKK
                     RKIRETQMEAEASIRSKTYELRSADMQSDIDVENMRKEFVAINADNIRTLAEAEAQKV
                     GAVARMLKDVDPKLVQVLAAIGMEPSKLIAQAFGGLAENAGKIGQLNISPDLLESLMK
                     PGLEK"
     gene            1328..2263
                     /locus_tag="VFW59_00040"
     CDS             1328..2263
                     /locus_tag="VFW59_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362636.1"
                     /translation="MTSGADRKVVLVTRRTRLEELVIRFHTPAQARFYIEHLEQDFHE
                     YEMEHENYLAAKRTVLDVLAGVGRFQAIDRTFLPNFLFGGDDVVLALGQDGLVANTMK
                     YLNAHALVGINPDPCRYDGVLLPFAPKDIKGILLEVMQDAREYKTVTMAMASLSDGQK
                     LYAVNDLFIGPKSHVSARYEIRFGKVSEVQSSSGIIVSTGLGSTGWMKSVATGSVAIA
                     QAMGGHAPREAYLPQPWDAGRLCFAVREPFPSRSSSASLVFGVVTSGQPLVLVSKMPE
                     NGVIFSDGIEADFLEFNAGTEAVIGIAGRVGRLVI"
     gene            2313..3137
                     /locus_tag="VFW59_00045"
     CDS             2313..3137
                     /locus_tag="VFW59_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362637.1"
                     /translation="MNKKIALLVIDPQNDFCDIPERERNDFAPALPVAGADADMKRLA
                     AFIERAGAAIGSIHVTLDSHQPVDIAHPAWWVDGRGEAPAPFTLITATDIANGVWRAR
                     DASMQQDSLAYAQALEASGRYVLVVWPEHCLIGTWGNNIHSAVKRAIDKWGREQMAQV
                     DYILKGMNPKTEHYSAIRAELPDGNDPSTMPNRSLLQKLSAADTVIVAGEALSHCVAN
                     TVRDIANDIGEENIRKLVLLADCASPVTGFEKLGADFLDEMRARGMQSAMSVSLAI"
     gene            3152..4357
                     /gene="pncB"
                     /locus_tag="VFW59_00050"
     CDS             3152..4357
                     /gene="pncB"
                     /locus_tag="VFW59_00050"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF003704.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX5362638.1"
                     /translation="MNLKPIIQSLLDTDLYKFTMLQVFLHSFPAAEGEYRFKCRNKPG
                     VPLSKFREEIEAQIDHLCTLRFRPEELGYLRGLRFIKPDFVDFLELFQFRRRFISVKE
                     SADKEGLDIVAKGPLVSVMLFEIFVLSIVSEVYFSQFNKESRLEEGRARLRQKIMLIK
                     EFGKEAPLRNPFVFFDFGTRRRAWRDWHEEVVTTLVRDVPEFFKGTSNVDMARRFGIV
                     PIGTMAHEYLQAHQAFGYRLRDFQSMALENWVKEYRGDLGIALTDVITMDAFLADFDL
                     YFAKLFDGLRHDSGDPFEWGEKALQHYERLRIDAGTKMLTFSDSLDLPKAFELYRRFA
                     GRARTGFGIGTNLTNDLGVEPLNLVMKLVSCNDNPVAKISDAPGKTLCDNETFIAYLR
                     QVFEVKPGA"
     gene            4398..>5345
                     /locus_tag="VFW59_00055"
     CDS             4398..>5345
                     /locus_tag="VFW59_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5362639.1"
                     /translation="MEPLNITMAQLDFRICDFARNVGMINAVIRANASSDLIVFSELA
                     LSGYYPQDMLDEPAFIERQDAALAEILAASRHTGAAIVLGAVTRNAGVGKPFHNSLLV
                     IRDGKVLLSYQKQLLPTYNIFDEQRHFAPGENRAAVFTLKERRIGFLICEDGWNDEEL
                     DYEVNPFSTLTEASVDLIVSINASPSDVGKQRQRHGIFGSASKRHGVPLLYVNQVGGN
                     DQLVFDGGSFVVDPQAGVVHEMPLFEEAVEQVSFDGAFFNARGRLDSPVRDYLADEEF
                     YYRQIVLGLRDYLEKTGFKSVVVGSSGGIDSALTLALACD"
BASE COUNT         1136 a         1452 c         1642 g         1115 t
ORIGIN      
        1 gtcaacaaag ccccctgcca gcgcccactt gttcgggaac ggctcgccgg gatcgttatt
       61 ccgctgcacc agcaaaacgt gcaagcgatc cgagatgatc gtgaatatcg ccacatccac
      121 ggtggcaagc gggcgctgga aattcaacgg gccggttccg gctgaatctt ttggggtggt
      181 tgctttcatg gatttcggtt tgttcgttgc ggttgttgac agtctatatt tcttagtggt
      241 actgtgcaac taacttagtg cgtcacagca actaagtatt ctgaaaagga gaacatcatg
      301 tttggcatac gattcatcaa agcagaacca accacctacc tcatggcttt ccggggaggg
      361 aagatcgtca aggaaggagc cggattgagc ctcctgtatt tcgggccaac cacttcgctg
      421 gtggctgtgc cggtaggtag ccgtgctctg gactttatct ttcaaccagt cacgtccgac
      481 tttcagtcgg tgactgtgca aggccaagtt tcctatcgcg tgagcgaacc tcggaagttg
      541 gccggcatgc ttgattactc tttgcgttct gacggtcggt atgcttccga tgacccggaa
      601 aaactcaggg atcgcgtctt gggcgtggct gaagtgctga ttcaaaaaaa cctgaagaaa
      661 atggatttga agcaggcctt gcttgcctcg gatgagattg cccttgtgat cgaagacggg
      721 ctgcgcggac aggcggaaat ccagtcgctc gggattgatg tcttgcgcgc ttccgtgctg
      781 gcgatcaagc ccacgcccga aacagccaag gcattggagg cggaagcgcg cgagtcgatt
      841 ttgcggcgtg cggacgaggc ggtgttctcg cgccgtaatg cggcagtcga gcaggagcgc
      901 gccatacgcg aaagcgagct ggatacggaa gtggcggtcg agcagaaaaa acgcaaaatc
      961 cgcgagaccc agatggaggc tgaggcgagc atccgtagca agacgtacga gttgcgctcc
     1021 gccgacatgc agtccgatat cgatgtggaa aacatgcgca aggagttcgt ggcgatcaat
     1081 gcggacaaca tccgcaccct ggctgaggcg gaagcacaga aagtcggggc agtcgccagg
     1141 atgctcaagg atgtcgatcc gaaattagtt caggttttgg cggccatcgg catggaaccc
     1201 agcaagttga tcgcccaggc attcggcggt ctcgcggaga acgccggtaa gatagggcag
     1261 ttgaatattt cgcctgactt gctcgaatcg ttgatgaagc ccggtttgga gaaataggag
     1321 agatcaaatg acatcgggag cggacaggaa ggtggtattg gtgaccagac gaacccgtct
     1381 ggaggagttg gttatccggt ttcatacgcc agcccaagcc aggttctaca tcgaacactt
     1441 ggagcaggac ttccatgaat acgagatgga gcatgagaac tatcttgccg caaagcgtac
     1501 ggtgctggac gtgttggccg gtgtcggtag gttccaggca attgatcgga catttttgcc
     1561 gaacttcctg ttcgggggcg acgatgtggt gttggcgttg gggcaagacg ggcttgtggc
     1621 caacaccatg aagtacctga atgctcatgc gctggtaggt atcaatcccg atccctgccg
     1681 atacgacggc gtcctgcttc cgttcgcgcc caaggacata aaaggaatat tgctggaagt
     1741 gatgcaagat gcgagagagt acaagactgt caccatggcg atggcatcgc tcagcgatgg
     1801 gcagaaactg tatgcggtca acgacctgtt catcggcccg aagtcgcatg tctcggcccg
     1861 ctacgagata cggttcggca aggtatccga ggtgcagtca tccagcggca tcatcgtttc
     1921 gacgggcttg ggctcgaccg ggtggatgaa gagcgttgcg acaggttctg tcgcaatcgc
     1981 tcaggccatg ggaggacatg cgccgagaga ggcttatctg ccgcaaccct gggatgcggg
     2041 ccggttgtgc ttcgctgtgc gtgaaccgtt tcccagtcgg tcatccagtg cgtcgttggt
     2101 gttcggcgtc gtcacctccg ggcagccgtt ggtgcttgtt tcgaaaatgc ctgagaacgg
     2161 agtgatcttc agcgacggca tcgaagccga ctttctggag ttcaacgcag gcacggaggc
     2221 tgtcatcgga attgcaggac gagttggtcg cctggtgata tagcaatgga caggaacaaa
     2281 atttattgag cgtcaggaaa aaggagagag ccatgaacaa aaagatagcc ctattggtga
     2341 tcgatccgca gaacgatttc tgcgacatcc cggagcgcga gcgcaacgat tttgcgcctg
     2401 cattgccggt tgccggagcg gatgccgaca tgaagcggct ggccgccttc atcgagcgtg
     2461 ccggtgcggc tatcggcagt atccatgtca cgctggattc gcaccagcct gtcgatatcg
     2521 cccatcctgc gtggtgggtc gacgggcgcg gcgaagcgcc cgcgccattc acgctcatca
     2581 ccgcaaccga catcgccaac ggtgtctggc gcgcccgcga tgcgtccatg cagcaggaca
     2641 gtcttgccta tgcacaggcg ctcgaagcat ccgggcgtta cgtgctggtc gtctggccgg
     2701 agcactgcct gatcggcacc tggggcaaca acatccacag cgccgtcaaa cgcgccatcg
     2761 acaaatgggg ccgggagcag atggcgcagg tggattacat cctcaagggc atgaacccga
     2821 agaccgagca ctactccgcg atccgggccg aactgccgga cggcaacgat ccttcgacca
     2881 tgccgaatag gtcgctgttg cagaagctgt cggcggcgga caccgtcatc gtggcaggtg
     2941 aggcgctctc gcactgcgtg gcaaatacgg tgcgcgacat tgccaacgac atcggcgaag
     3001 agaacatcag gaaattggtg ttgctggccg actgtgccag tccggtgacc ggcttcgaga
     3061 aattgggcgc agacttcctg gacgaaatgc gcgcgcgcgg gatgcaaagc gcaatgtccg
     3121 tcagcctggc aatttgaaaa aaggaaacat catgaacctc aaacccatca tccagagctt
     3181 gctcgatacc gacttataca agttcaccat gctgcaagtc ttcctgcaca gcttccccgc
     3241 ggcggagggc gagtaccggt tcaagtgccg caacaagccg ggcgtgccct tgtcgaaatt
     3301 ccgggaggag atcgaagcgc agatcgatca tttgtgtacg ctgcgcttcc gcccggaaga
     3361 gttgggctac ttgcgcggcc tgcgctttat caaacctgac tttgtcgatt ttctggagtt
     3421 gttccagttc cgtcgccgct tcatcagcgt gaaggagtcc gccgacaagg aagggctcga
     3481 catcgtggcc aaggggccgc tggtttccgt gatgttgttc gagatattcg tgctgtccat
     3541 cgtcagcgaa gtctatttca gccagttcaa caaggaatcg cgactggagg aggggcgcgc
     3601 gcgcttgcgg cagaagatca tgctgatcaa ggagttcggc aaggaagcgc cgctgcgcaa
     3661 tcccttcgtg ttcttcgatt tcggcacgcg ccgccgggcc tggcgggatt ggcacgagga
     3721 ggtggtgacc acgctggtca gggacgttcc cgagttcttc aagggcacca gcaacgtcga
     3781 tatggcgcgt cgcttcggca tcgtgccgat cggcaccatg gcgcacgaat acctccaggc
     3841 ccatcaggcc ttcggctatc gcttgcgcga tttccaaagc atggcgctgg aaaactgggt
     3901 caaggaatat cgcggcgacc tcggcatcgc cctgaccgat gtgattacca tggatgcctt
     3961 cctggcggat ttcgatctct acttcgccaa gctgttcgat ggcttgcggc acgattccgg
     4021 cgatccgttc gagtggggcg agaaagcgtt gcagcattac gagagattgc gcatcgacgc
     4081 cggaaccaag atgcttacgt tctcggacag tctcgatctg ccgaaggcgt tcgagctcta
     4141 tcgccgcttt gccgggcgtg cccggaccgg cttcggcatc ggcacgaacc tgaccaacga
     4201 tctcggtgtc gagccgctca atctggtgat gaagctggtg agttgcaacg acaatcccgt
     4261 ggccaagatc tcggatgctc cgggcaagac tctgtgcgac aacgaaacct tcatcgccta
     4321 tttgcgccag gtgttcgagg tgaaaccagg tgcctgatga gaaatcatgt gtttgatgag
     4381 aaacaagcga ggtgaatatg gaaccgctca acataacgat ggcccagctc gattttcgca
     4441 tctgcgattt tgcgcgcaat gtcggcatga tcaatgccgt catcagggca aatgcctcct
     4501 ccgatttgat cgtgttttcg gagcttgcgc tatccggcta ttacccgcag gacatgctcg
     4561 acgaacccgc ttttatcgag cgccaggatg ctgcgctggc ggagatactt gcggcatcgc
     4621 gccacaccgg ggcggccatc gtcctcggcg cggtgacgcg caacgccggg gtcggcaagc
     4681 ctttccacaa cagcttgctg gtgatccggg acggcaaggt gctcctgagt taccagaagc
     4741 agcttttgcc cacttacaac atcttcgacg agcagcgcca cttcgcgccc ggcgaaaacc
     4801 gggccgccgt gttcacgctc aaggagcgca ggataggctt cctgatctgc gaagacgggt
     4861 ggaacgatga ggaactcgat tacgaagtga atccgttcag cacgctgacc gaggcaagcg
     4921 tcgatctgat cgtcagcatc aacgccagtc cttccgacgt cggcaagcaa cgtcaacgcc
     4981 acgggatatt cggctcggcc agcaaacgcc atggcgtgcc cttgctctat gtgaaccagg
     5041 tcggcggaaa cgaccaactg gtgttcgacg gcggctcgtt cgtcgtcgat ccgcaggcgg
     5101 gtgtcgtgca tgagatgccg ctgttcgagg aggctgtcga gcaagtgtct ttcgacggcg
     5161 cattcttcaa tgcgcgcggc aggctcgatt cgccggttcg ggattatctt gccgacgagg
     5221 aattctatta ccgccaaatc gtgctgggct tgagggatta cctcgaaaag accggcttca
     5281 agagcgtcgt cgtcggctct tccggcggca tcgactcggc gctcacgctg gcgcttgcct
     5341 gcgat
//
LOCUS       DASUIV010000003         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_93861, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000003 DASUIV010000000
VERSION     DASUIV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93861"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFW59_00060"
     CDS             <1..333
                     /locus_tag="VFW59_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5362640.1"
                     /translation="GPGTWVYGLSMALGRRVLASNPQVNVFHRGFKACDSYANGETAI
                     QQITCPVLFVLGAQDQMTPPKSAQGLITTARNAGKAVQVASLAVGHHQMNEAPDATLF
                     AIRDFLKT"
     gene            complement(497..1048)
                     /locus_tag="VFW59_00065"
     CDS             complement(497..1048)
                     /locus_tag="VFW59_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362641.1"
                     /translation="MFNKEHFIEDCINSVAEGQGAIREVVAEAVSDSVRVMSELGEPE
                     HAGITPIYRSRDLTIINFVWAPYMILMPHNHQMFSVVGIYSGREDNVFWRRTATTIDA
                     VGAKSLGVGDVAALGHDIIHSVLNPIGKMTCAIHVYGGDFFHPDEPRSEWDHETLIER
                     PWNIDKVKSIFREAETRFNATKK"
     gene            complement(1073..1855)
                     /locus_tag="VFW59_00070"
     CDS             complement(1073..1855)
                     /locus_tag="VFW59_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5362642.1"
                     /translation="MLKVVMNLFSLMSRRVKMAITNTQSGTNVHEVANGIYRINTPVF
                     VAGGPGGFSFNQYLIVDDEPLLFHTGPRKMFPLVREAVASILPVEKLRYVAFSHVEAD
                     ECGSLNEWLAITPQSSPLCGTVAALVSITDMADRPPRALADGELLSLGTHAVRWFDTP
                     HLPHAWECGLLMEESSRTLLCSDLFTQPGSDHPPLTESDILGPSEAFRQAMDYYSHTS
                     KARAMIERLASAEPTTLACMHGSAWHGDGAKLLRALANVLSP"
     gene            complement(1892..>2851)
                     /locus_tag="VFW59_00075"
     CDS             complement(1892..>2851)
                     /locus_tag="VFW59_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5362643.1"
                     /translation="WVSRAVYVAARLGLADLLDNGPKTGQELAELTQTHAPSLYRLLR
                     ALASLGLFTESAEHRFALTPLGATLKTGAPGAARSTVMALAGPWMWGAWGEFLYSVQT
                     GKTAFDKVWGMPVFDYLAQHADEARFFGEAMTGVHGSEPPAVAAAFDFSEIRTVVDVG
                     GGTGTLLATILRAHPHLRGVLYELPHVVPEARANLTESGVGERGSVIAGNFFESVPAG
                     GDAYVLSHVIHDWDEEKCLTILRNCQQAMARNGRLLMVECVLPSGDAPHLGKILDLVM
                     LTVPGGVERSGEEYASLLARAGFRLTRIVPTASAVSIVEAMPA"
BASE COUNT          621 a          863 c          792 g          575 t
ORIGIN      
        1 ggcccgggaa cctgggtcta tggtctgagc atggcgctgg gccgccgcgt actggccagc
       61 aacccccagg tcaacgtgtt ccatcgtggc ttcaaagcct gcgacagcta tgccaacggc
      121 gaaaccgcta ttcagcagat cacctgcccg gtgctgtttg tgctcggcgc ccaagaccag
      181 atgacgccgc ccaaatccgc gcaaggcctc atcaccacgg cgcgcaatgc aggcaaggcc
      241 gtgcaggtcg caagcctggc agtcggtcac catcagatga acgaggcgcc ggatgcgacg
      301 ctgtttgcga ttcgggattt cctgaaaacc tgagcacgcc cgccgacttc ttcgccaccg
      361 tttgacgaca ggcgatgcaa gggttgggcg gaatccaggg gcaaggtcat cccttcatcc
      421 tgggcagcgg tttcttatac gcgaaggatc tcgcccttga gcgaatagag agcttcacag
      481 tgccggatca ttagcgctat ttctttgtgg cattaaatcg tgtctctgct tctcgaaata
      541 tagatttgac cttgtcgata ttccatggcc gttcgattaa tgtttcgtga tcccattcgc
      601 ttctcggttc atccggatga aaaaagtcac cgccataaac atgtattgca cacgtcattt
      661 tgccgattgg attaagcacc gaatggataa tgtcgtgacc gagagccgct acgtcgccga
      721 ctcccagaga tttggcgcct accgcatcaa tggttgttgc tgttcttcgc caaaaaacgt
      781 tgtcttccct gccggaatat atgccgacca ctgaaaacat ctggtgattg tgtggcatga
      841 gaatcatgta gggcgcccag acgaagttga tgatggttag atcacgcgat ctatatattg
      901 gtgtaatacc tgcatgttcc ggctcaccaa gctcagacat caccctcacg ctgtcggaaa
      961 ccgcttcggc aacaacctcg cgaattgcgc cctgaccctc agcaacagaa tttatacaat
     1021 cctcgatgaa atgctcttta ttgaacatga tttcatttct ttctctcttg gtttatggag
     1081 acagtacatt cgcgagagca cgcaggagct tcgcaccgtc gccatgccat gcgctgccgt
     1141 gcatgcaagc cagcgtggtg ggctcggcag aagcaagtct ctcaatcatt gcccgcgcct
     1201 tgctggtgtg ggagtaatag tccatcgcct ggcggaacgc ttcgctcggc cccagaatgt
     1261 ccgactcggt gaggggtgga tgatcgctgc cgggctgcgt aaacagatcg ctgcacagca
     1321 aggtccggct gctttcctcc atcagtaaac cgcattccca cgcatgcggc aagtgcggcg
     1381 tatcgaacca ccgcactgca tgcgtgccga gcgaaagcaa ctcgccgtcg gcaagcgcgc
     1441 gaggcggtcg gtcagccatg tcagtgatcg ataccaatgc cgcgactgtg ccgcacagcg
     1501 gcgaggattg cggcgttatt gcaagccatt cgttgagcga cccgcactca tccgcttcaa
     1561 cgtgcgaaaa cgcgacgtaa cgaagttttt ccaccggaag gatgctcgca actgcctcgc
     1621 gaaccagtgg gaacatcttg cgcggccccg tgtgaaaaag cagcggttca tcatcaacga
     1681 tgagatattg attgaatgaa aacccgccag gccctccggc cacgaacacc ggcgtattga
     1741 tgcggtagat tccgttcgca acctcatgca cattggtacc ggattgcgta ttggttatgg
     1801 ccattttcac tctccttgac atcagggaaa aaagattcat gacaactttc agcatcggct
     1861 ggaagcatcc atcagcaaca cgactcccgc ttcacgcggg catggcttcc acgatgctca
     1921 ccgccgaggc cgtcgggacg atacgtgtca gccgaaagcc ggctcttgca agcagcgagg
     1981 cgtactcctc acccgagcgc tcgacaccac ctggcacggt caacatcacg agatccagta
     2041 tcttgcccag atgaggcgca tcccccgaag gaagcacgca ttcaaccatg agcaggcgac
     2101 cattgcgcgc cattgcctgt tgacagttac gcaggatcgt gaggcacttt tcttcgtccc
     2161 agtcatggat gacgtgggac aagacatagg catcgccacc cgccggcact gactcgaaga
     2221 agttgccggc gatcacactg ccccgctccc cgacgccgga ttccgtcagg ttcgcgcgcg
     2281 cctcaggcac aacatgcggg agttcgtaca ggacgccgcg gagatgcggg tgggcacgca
     2341 ggatcgtcgc cagcagggtg ccggttccac cgcccacatc aacgacggtc cggatttccg
     2401 aaaaatcgaa ggctgccgca acagcggggg gttcgctgcc gtggacaccg gtcatggcct
     2461 cgccgaagaa gcgggcctcg tcggcatgct gcgcaaggta gtcaaacacc ggcatgcccc
     2521 ataccttgtc gaaagcagtt ttcccggtct gcacgctgta tagaaactca ccccacgctc
     2581 cccacatcca ggggcccgca agggccatca cggtcgatcg ggcggctccc ggagcgccgg
     2641 ttttcagcgt ggcacccagc ggcgtgagcg caaagcggtg ctcggcactt tccgtaaaca
     2701 aacccaggct cgccaaggcg cgcaacaggc ggtagaggga cggggcatgc gtctgcgtga
     2761 gttcggcgag ttcctggccg gtcttcggtc cgttatcgag cagatcagca agacccaaac
     2821 gggccgccac gtacacggcg cgggaaaccc a
//
LOCUS       DASUIV010000004         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_837318, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000004 DASUIV010000000
VERSION     DASUIV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837318"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFW59_00080"
     CDS             complement(<1..458)
                     /locus_tag="VFW59_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX5362644.1"
                     /translation="MLALLAELPFGGQVAIAALLGLLLGSFLNVVIYRLPLMLERRWA
                     AECAQWQGSEVVEQPPFNLLVPASACPHCQAPIRAWQNIPLLSYLLQRGRCSHCRAPI
                     SWRYPLVELASGLLFAGFAWHYGLSPWWLVASLFSVTLLTLALIDARTQL"
     gene            complement(458..1675)
                     /locus_tag="VFW59_00085"
     CDS             complement(458..1675)
                     /locus_tag="VFW59_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5362645.1"
                     /translation="MPTPSKKPASHVFVWEGRDRAGKAIRGEIRAESETVARAQLRRQ
                     GVSPTKLKKRSASLRQRITEKDVALFTRQLATMMKAGVPLLQAFDIAAKGHSNAAFVR
                     ILLDVRSEVESGSNLADAFRKYPLYFDKLFCNLIAAGEAGGVLDALLDKLATYKEKVI
                     AIKGKIKSAMIYPAAIIATAFIITAVILIYVIPAFKELFSSFGADLPAPTQIVIWLSD
                     LFVEYWWMVFGSIAVVIIGTVKLYQRSPKMQAAMDRFVLKVPVIGDVVRKATIARWAR
                     TLSTLFTAGVPLVEALDSVAGAAGNQVYADATKVIQSDVSAGSTLNNSMQRSNLFPNM
                     VLQMTSIGEESGSLDSMLDKVADFYEDEVDNAVASLSSLLEPMIMIILGVLIGGLVIA
                     MYMPIFQMGQVVG"
     gene            complement(1692..>3198)
                     /gene="pilB"
                     /locus_tag="VFW59_00090"
     CDS             complement(1692..>3198)
                     /gene="pilB"
                     /locus_tag="VFW59_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX5362646.1"
                     /translation="DLAALSQDGLPRNLVDDDTVNNGRLLPLYKRGNKLFIATSDPTQ
                     ATLFHTITFKTGLSIDLVVVEDDKLARAIGRYASNPSKVLNDLAGDEFGELQLTDGDA
                     QLESDSLPDVDDAPVVKFVNKVLMDAINGGASDIHFEPYEKFFRIRYRVDGILKEIAQ
                     PPLAIREKLASRIKVVSKMDISEKRVPQDGRLKLVISKHRAVDFRVSTLPTLYGEKIV
                     MRILDSSSAALDIEQLGFEPEQKEMLLRAIERPYGMVLVTGPTGSGKTVSLYTCLNIL
                     NQPDSNISTAEDPVEINLPGINQVNVNEKAGLTFASALRAFLRQDPDIIMVGEIRDFE
                     TADISIKAAQTGHMVFSTLHTNNAPATLTRLLNMGVAPFNVASSVLLIMAQRLARRLC
                     PHCKAPVEIPEPALLRAGFRKEELDGSWQPHGPVGCEECRGSGYKGRVGIYEVMPITD
                     AMVRLIMSGGNAMDIADLARQEGMVDLRHAGLLKVKRGLTSLTEIEAVTNE"
BASE COUNT          642 a         1048 c          928 g          580 t
ORIGIN      
        1 aacagctggg tacgggcatc gatcagcgcc agggtcagca gcgtcaccga aaacagcgac
       61 gccaccagcc accacggcga caggccgtag tgccaggcaa agccggcaaa cagcagcccg
      121 ctggccagct ccaccaatgg ataacgccag gaaatcggtg cccggcaatg gctgcaacga
      181 ccgcgctgca gcaggtaact gaggagcggg atgttctgcc acgcgcggat cggcgcctgg
      241 caatgcgggc aggccgacgc cggcaccagc aggttgaacg gcggctgttc caccacctcg
      301 ctgccctgcc actgcgcgca ctccgccgcc cagcgccgtt ccagcatcaa gggcagacga
      361 tagatcacca cgttgagaaa actgccgagc agcaggccca gcagtgccgc aatcgccacc
      421 tggccgccga acggcagctc cgccaatagc gccagcatca accgaccacc tgccccatct
      481 ggaagatcgg catgtacatc gcgatcacca agccaccaat cagcacgccg agaatgatca
      541 tgatcatcgg ctccagcaag ctggacagcg acgccaccgc attatcgact tcgtcttcat
      601 aaaaatccgc caccttgtcc agcatggagt cgagcgagcc ggactcctcg ccaattgagg
      661 tcatctgcaa caccatattg ggaaacagat tggaacgctg catcgagttg ttgagggtcg
      721 agccggcgct gacatccgac tggatgacct tggtggcatc ggcatacacc tggttgccgg
      781 cggcaccggc caccgagtcc agcgcctcca ccagcggcac gccggcggta aacagcgtcg
      841 acagcgtccg cgcccagcgc gcaatggttg ccttgcgcac cacatcgcca atcaccggca
      901 ccttcagcac gaaacgatcc atcgccgcct gcatcttggg cgagcgctgg tacagcttga
      961 cggtaccgat gatcaccacc gcgatggagc cgaacaccat ccaccagtac tcgacgaaca
     1021 ggtcggacag ccagatcacg atctgcgtcg gcgccggcag atcggcacca aaactggaga
     1081 acagctcctt gaacgccgga atcacataga tcaggatcac cgcggtaatg atgaaggcgg
     1141 tggcgataat cgccgccgga tagatcatcg ccgacttgat cttgcccttg atggcaatca
     1201 ccttttcctt gtaggtcgcc agcttgtcca gcaaggcatc cagcaccccg cccgcctcgc
     1261 cggcggcgat caggttgcag aacagcttgt cgaagtacag cggatacttg cgaaaggcat
     1321 cggccaggtt actgccggat tcgacctccg agcgcacatc gagcaggatg cgcacaaagg
     1381 cggcattact gtggcccttg gccgcaatat cgaaggcctg caacagtggc accccggcct
     1441 tcatcatcgt cgccagctgg cgggtaaaca gcgcgacgtc cttttcggtg atgcgctgcc
     1501 gcaggctggc gctacgcttc ttcagcttgg tcgggctgac cccttgccgt cgcaactggg
     1561 cacgggccac ggtttccgac tcggcacgaa tctcgccgcg gatggccttg ccggcccggt
     1621 cgcgcccttc ccagacaaag acatgggagg cgggcttctt gcttggcgtg ggcatagtga
     1681 tcgcttgtct attattcgtt ggtaacggct tcgatttcag tcagcgacgt caggccacgt
     1741 tttactttca gcaggccggc atggcgcaaa tcgaccatgc cctcctggcg cgcgaggtcg
     1801 gcaatatcca tcgcgttgcc gccgctcatg atcagccgca ccatggcgtc ggtgatcggc
     1861 atcacctcgt agatgccgac ccgccccttg tagccactgc cgcgacactc ctcgcagccg
     1921 accgggccgt gcggctgcca gctgccgtcc agctcttcct tgcgaaagcc ggcacgcagc
     1981 aaggccggct cgggaatctc gaccggtgcc ttgcagtgcg gacacagccg tcgcgccagc
     2041 cgctgcgcca tgatcagcaa taccgaactg gcgacgttga acggcgctac gcccatgttc
     2101 agcagtcggg tcagtgtcgc cggggcatta ttggtatgca aggtagaaaa taccatgtgc
     2161 ccggtttgcg ccgccttgat cgagatatcc gcggtttcaa agtcgcggat ctcgccgacc
     2221 atgatgatgt ccggatcctg acgcaggaag gcgcgcagcg ccgaggcaaa ggtcagcccg
     2281 gccttctcgt tgacattgac ctgattgatc ccgggcaggt tgatctccac cgggtcttcc
     2341 gcggtagaga tattgctgtc cggctgattc aggatattga ggcaggtata cagcgacacc
     2401 gtcttgccgc taccggtcgg cccggtcacc agcaccatgc cataagggcg ctcgatcgcc
     2461 cgcagcagca tttccttctg ctccggttcg aaaccgagct gttcgatatc cagcgcggca
     2521 ctggaagagt cgagaatacg catcacgatc ttctcgccat acagcgtcgg caaggtgctg
     2581 acccggaaat ccacggcacg gtgcttggag atcaccagct tcaggcggcc gtcctgcggc
     2641 acccgctttt ccgagatgtc catcttcgac accaccttga tgcgcgacgc cagcttctcg
     2701 cggatggcca gcggcggctg cgcgatctcc ttcaggatac cgtcgacgcg atagcggatg
     2761 cggaagaact tctcgtaagg ctcgaagtga atgtcggatg ccccaccatt gatggcgtcc
     2821 atcagcacct tgttgacgaa cttgaccacc ggtgcgtcat cgacatccgg caggctgtcg
     2881 ctctccagtt gcgcatcgcc atcggtcagc tgcagctcgc caaactcgtc gccagccaga
     2941 tcgttgagca ccttgctcgg attgctggca tagcggccga tcgcgcgtgc taacttgtca
     3001 tcctccacca ccaccaggtc gatgctgagg ccggtcttga aggtaatggt gtggaacagc
     3061 gtcgcctgcg tcggatcgga ggtggcgatg aacagcttgt tgccgcgctt gtacagcggc
     3121 agcaagcggc cgttattgac cgtgtcatcg tccaccagat tgcgcggcag tccgtcctgg
     3181 ctcagcgcgg ccagatcc
//
LOCUS       DASUIV010000005        17523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_93940, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000005 DASUIV010000000
VERSION     DASUIV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 17523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17523
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93940"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFW59_00095"
     CDS             complement(<1..482)
                     /locus_tag="VFW59_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX5362647.1"
                     /translation="MKLMHLLLASALLTGCAGEEFQDLRDFVQNAGADLRGKIPPPPE
                     VKPYEPFVYDNVDNLQDPFKPRKPDSRSASRPGLNQPDMDRPKEALEEFPLEGLHMVG
                     YLRQNKVGYAIIRAPDGKLHRVRAGNYVGMNFGLIKEVGETEIVVQETVQDSAGDWSE
                     R"
     gene            complement(479..1099)
                     /locus_tag="VFW59_00100"
     CDS             complement(479..1099)
                     /locus_tag="VFW59_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX5362648.1"
                     /translation="MMNLDELKNLNPKNPGAWPWPAKILAFLVLFLSVVAAGAFLDWQ
                     EQWNSLQAIKREEQTLKDTFLVKKKQAINLDLIKKQLTETQESFGALLKQLPSKSEMD
                     ALLTDINQAGLGRGLQFELFRPGAETVNGAFAEQPITIKVTGNYDDIGKFSSDISMLP
                     RIVTLNEISVSPLGTALSMDAIAKTFRYLDEEEVASQKKATGAKKP"
     gene            complement(1096..1650)
                     /locus_tag="VFW59_00105"
     CDS             complement(1096..1650)
                     /locus_tag="VFW59_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX5362649.1"
                     /translation="MIRINLLPHRAEKRRARQIQFGAFAVISVLLGAMLVGFVHVAIG
                     TQISYQERRNEYLKQETAALDRQIAEIRKLREQTDSLLARKNVVEGLQSTRSDVVHLL
                     DQMLRILPDGVYLKSLRQTGNKISVVGYAQSNARISTLMRAIEDSPWLDTPSLVEIHA
                     SSANGARVSEFSLTFNLTKTQVQP"
     gene            complement(1647..2747)
                     /locus_tag="VFW59_00110"
     CDS             complement(1647..2747)
                     /locus_tag="VFW59_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX5362650.1"
                     /translation="MPKVNFDIPLDFLRATTPPLIGVDISASSVKMVELSETPKKTGY
                     VVERYVIEPLPKDAVSDGNIVNLDAVAESLQRAWKRMGVRIKNVALALPAAAVITKKI
                     LLPAGMREEDLEYQVESEANQYIPFALEEVNLDFQVVGPAPGNPDEIEVLLAASRKAN
                     VEDRVAAAEVAGLKAVVIDVEPYAAQMAFEQIRMQLPDGAEDKCVALVDIGASVMSVN
                     VLRNGQSVYARDQQVGGDQLTQQIQAVFGLSAEDAEAAKRNGGLPDNYESDVLSAFRE
                     SVVMEIARALQFFFTSTQYNEVDYIVLAGGCAVLPGLDDAVATRTQVSTMVANPFALM
                     TLSGRIKPRQLQADAPALIVACGLAMRRFDPS"
     gene            2893..5208
                     /locus_tag="VFW59_00115"
     CDS             2893..5208
                     /locus_tag="VFW59_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX5362651.1"
                     /translation="MTSRRWLLLLIPLTLLLLPAILFGLAVTLTYPTLPSLDALTDYR
                     PKIPLRVYSADGALLGEFGEERRALVNIAEVPDLMKNAILAAEDDRFYQHGGVDYLGV
                     LRAAVSNFTSGGAKQGASTITMQVARNFFLSKEKTFSRKFNEVLLAFKIERNLSKDRI
                     LELYFNQIYLGQRAYGFATAAQIYFGKSLNQLTVAEAAMLAGLPKAPSAYNPVVNPKR
                     AKLRQLYVLRRMHELNFINDEQFQLAQQQPLVAKHGNQEFGTKSDFIAEMVRQAIYEQ
                     YHEDTYTSGLKVYTTIRQQDQLAAYQAVRKNALDYDHRHGYRGPESFVDLSTGNSEEQ
                     LEEALQDVTDNDDLIPAVVLAASPKLVRAYCKGGEVAEVSGDALAFAQRALNGKVALN
                     QRIIPGSIIRLQKDDKNNWQITQLPQVEAAFVSADPRNGAILALVGGFDFNHNQYNHV
                     TQAWRQPGSSIKPFIYSAALEKGFTPATLINDAPLLFDATQTGSDPWQPKNYDGKFEG
                     PMRMREALTKSKNMVSIRILQSITPQYAQDYLTKFGFDADKHPPYLTMALGAGMVTPM
                     QMLGGYSVFANGGFRIQPYFISRIEDANGKVLSRATPVVAAENAEQVIDVRNAYTMVS
                     MMQDVVRRGTATAAMRLGRSDLAGKTGTTSDSIDAWFCGFQPTVVGISWMGFDQPRSL
                     GDHETGGGAALPMWIAYMEKVLKDVPQAVYEMPENMAAVRINASGHRDENGSLIDYFY
                     QENIPSEQAAPPPPLPGSAVKPTETLKDQLL"
     gene            5213..5806
                     /locus_tag="VFW59_00120"
     CDS             5213..5806
                     /locus_tag="VFW59_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362652.1"
                     /translation="MNKEHGHRRDLMREQIAHQAAKLMAEGSITDHAFAKRKAAKQLG
                     AADTQHLPSNLEVDDALHSYRALYQRDSHPAILRQLRDEALSTMRQLAVFHPYLTGSV
                     LTGTAGVQSDINLMLFSDDEKAVLLFLLKHKLEFEDGEWRVRIAGREESVPSYTLTAE
                     SGIQTHVIVLPENARHSGSRHPETHADISAVEALLAG"
     gene            complement(5923..6927)
                     /gene="hemB"
                     /locus_tag="VFW59_00125"
     CDS             complement(5923..6927)
                     /gene="hemB"
                     /locus_tag="VFW59_00125"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5362653.1"
                     /translation="MQTLGQYPHKRMRRMRHDQFSRDLMREHVLTSADFIYPVFVLEG
                     ANKVEDVKSMPGVQRKTLDLLLKDAEQCVKLGVPVMAIFPVIDVAQKSLDAREAYNPD
                     GLVPRVVAELKKHFPELGVMTDIALDPYTSHGQDGLIDDSGYVLNDETIAVLTKQAQC
                     HAAAGADIVAPSDMMDGRIGAVRAALDTNGHIHTRIMAYSAKYASSFYGPFRDAVGSS
                     GNLGKGNKYTYQMDPANSDEALWEVGLDLQEGADMVMVKPGMPYLDILRRVKDEFKAP
                     TFVYQVSGEYAMLKAAAQNGWLDERACVLESLLAFKRAGADGILTYFALDAAKWLKQG
                     "
     gene            complement(7113..7730)
                     /gene="yihA"
                     /locus_tag="VFW59_00130"
     CDS             complement(7113..7730)
                     /gene="yihA"
                     /locus_tag="VFW59_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX5362654.1"
                     /translation="MGLFSQAAFYTTVNHMKDLPLHGGKEVAFVGRSNAGKSSAINTL
                     ANHVRLAYTSKTPGRTQHLNYFSLAEGKYLVDLPGYGYAKVPPDVQKHWEKLLGQYLQ
                     TRDELAGLVVIMDSRHPLTALDEGMLDWFAPTGKPVHVLLTKSDKLSRQQATKTLMEV
                     KAYLKEHFPNCSVQLFSSLKKQGIEEAEKVIAGWLAPEVSPVEPD"
     gene            complement(7730..9013)
                     /gene="hemL"
                     /locus_tag="VFW59_00135"
     CDS             complement(7730..9013)
                     /gene="hemL"
                     /locus_tag="VFW59_00135"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5362655.1"
                     /translation="MTSHNQQLFDESQKIIPGGVNSPVRAFKSVGGTPLFFKRGKGAY
                     VWDADDKQYVDYVNSWGPMIVGHCHPEVVKAVQAAAAEGLGFGAPTAAELEIAELLCK
                     ILPSLEMVRLVSSGTEATMTAIRLARGFTGRSRIIKFEGCYHGHSDGLLVKAGSGALT
                     FGQPSSSGVPAEIAALTTVLDYNDAAGLEKAFAEYGNEIAAVIVEPVAGNMNLILPKR
                     EFLDAMRKLCTKHGAVLILDEVMSGFRVGLQGAHGYYGIKPDLITLGKVMGGGLPAAA
                     FGGRRDIMQCLAPLGAVYQAGTLSGNPVAVAAGLTTLKLVQAPGFYEHLTQVAKQLTE
                     GLSASAAKHGIPFSAQSIGSMFGLYFSAHVPTSYAEVMASDKAAFNRFFHAMLDEGVY
                     LAPSAFEAGFVSAAHTEADIAATIAAADKVFAAWK"
     gene            complement(9036..9695)
                     /gene="thiE"
                     /locus_tag="VFW59_00140"
     CDS             complement(9036..9695)
                     /gene="thiE"
                     /locus_tag="VFW59_00140"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5362656.1"
                     /translation="MSEVETHRAVQRVIGGLYAITPDELDTAALLYKVRLALLGGAHL
                     LQYRNKLAPLPLRAEQAGALRRLTREFGVPLIINDDAVLARQVEADGVHLGGDDGSVA
                     EARALLGSGRIIGVSCYNRLPLAHEAVRQGADYVAFGAFFASGVKPAAVQAPLGLLQQ
                     ARQELHVPIVAIGGITVQNGAQLLRAGADALAVISAVFAAPDVQEAARQFADLNLSSS
                     R"
     gene            complement(9783..10616)
                     /gene="thiD"
                     /locus_tag="VFW59_00145"
     CDS             complement(9783..10616)
                     /gene="thiD"
                     /locus_tag="VFW59_00145"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX5362657.1"
                     /translation="MSESFPLVMTFAATDPSGGAGMQADLLTIASMGCHPLSVVTAIT
                     VQDTSGVDDILPIDSEWVEDQARAMLEDVPVAAFKIGLLGSVENIAAIAEVLADYPDV
                     PLVIDPVLASGRGDELADEDMLDAMRELLIPQATIITPNSMEARRLALDEDNEDDDPS
                     LDECAKRLLAMGCEYVLITGTHEQTAKVVNTLYSERGIVSSESWARLPGIYHGSGCTL
                     ASAIAALLAQDVDVPEAVKEAQEYTWQTLSAGFRPGMGQHIPDRLFWARGEEDAPDLP
                     N"
     gene            complement(10722..11516)
                     /gene="rsmA"
                     /locus_tag="VFW59_00150"
     CDS             complement(10722..11516)
                     /gene="rsmA"
                     /locus_tag="VFW59_00150"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5362658.1"
                     /translation="MSSHKAKKKFGQNFLVDERIIADIVAGIRPEPDDNMVEIGPGLG
                     ALTRPLLKRLNHLHVVEIDRDIIARLEHEYPQEAASRKLTIHAGDALQFDFATLPSPL
                     RIVGNLPYNISSPLLFHFAAYTERVKDMHFMLQNEVVERMVAEHSTPAYGRLSVMLQY
                     RFYMEKLLDVPPESFRPAPKVDSAIVRMIPLPRADIAVKNEKLYAQIVSAAFGQRRKT
                     LRNTLRDHLNEEDFARLGINSQLRAENLSVDDFIKVANYLDGKGLD"
     gene            complement(11503..12546)
                     /gene="pdxA"
                     /locus_tag="VFW59_00155"
     CDS             complement(11503..12546)
                     /gene="pdxA"
                     /locus_tag="VFW59_00155"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5362659.1"
                     /translation="MSPPFLAAPLAITAGEPAGIGPDLCVQLAVAAPDIPFVVIADKY
                     LLQQRAERLGISLQLREMVSGEWKVVSRGASATSHSLSVIHVPLAAPCQAGVLDAANG
                     DYVLATLRRALQGCQSGEFSGMVTAPVHKGIINDAGIPFTGHTEYLAEQTGTPLVVMM
                     LVGGGMRVALATTHLALRDVPDAITAALLEKVLRIIQCDLQRRFGIAKPRILVAGLNP
                     HAGEGGYLGREEIDVMIPVLDKLRAEGFEVSAPLPADTLFTPRKLAQCDCVLAMYHDQ
                     GLPVLKHASFGQGVNVTLGLPIIRTSVDHGTALDLAGTGRAEVGSLLEAIKLAAEMSQ
                     YEQFWFGHPHEQS"
     gene            complement(12543..13883)
                     /locus_tag="VFW59_00160"
     CDS             complement(12543..13883)
                     /locus_tag="VFW59_00160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5362660.1"
                     /translation="MMRMKCLMMCAAMLVWPLAHAADMPVVAPAVASDPQKQVAVIDA
                     VVAVVNDDVITRHELDDKLAVVVRQLQKQGTPLPAPDLLEKQILERMITDLLQAQFAK
                     ESGIRVDDTQLNLSLTRIAQQNNFTSLAEFRAKLESEGVDFNKFREEIRGEIISTRLR
                     EREVESKVVISDSEVDSYLANKAKMGGAEDELHLAHILVVVPEQASADRIQAAREKAE
                     QALAQLKGGADFAQVAAGASDAKDSLSGGDLGWRPATRIPPVLLEALQGLQPGETTAV
                     LRSSSGFHILKLIEKRSGVAQVVITQTHARHILIKTSEIVSDNEAKKRLMEIKARIEA
                     GADFAEQAKRYSQDGSAQQGGDLDWLSPGQTVPEFETAMNRLQPGMVGVAQSQFGWHL
                     IQVLERRNTDVSVQQKRQQARMALGTFKSDEQYQDWLRQLRDRAFIEYRLEENR"
     gene            complement(13898..16162)
                     /locus_tag="VFW59_00165"
     CDS             complement(13898..16162)
                     /locus_tag="VFW59_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEX5362661.1"
                     /translation="MRFRLKPVVLSLLCAFAQDAAANADLLALRMDKTFMAMKEPEQQ
                     TPSFLEADRLVGKQDDQVEATGNAVLRKRGQSISADRLLYFQNTSDLDAQGSVVVDQG
                     GNTMSGPHLKLNLDTDIGVMEQPVFYLIENDGRGSADVMHFQDKLHYQLDNATYTTCP
                     ADNEDWLLHMSGLELDRDRQIGTAHNAWVQFMGVPILYSPWMDFPLNDQRKSGFLAPI
                     IGSTTKGGSELTVPYYWNIAQNLDATIAPRVMAKRGVQFNNEFRYLEPGYSGELHVDV
                     LPSDMLTKSTRTRLGVLHGQNIYGGLNGYLNLNSVSDNNYYRDLASSVNVTSQINLMQ
                     EGVLSYGAGPWNAAMRVQRYQTLQDPAAPVAVPYKRLPQLTFNAAQNYSGASVSFASE
                     FVDFAHPTAVNGKRLVLNPAVSYPLISNQAFYLTPRAALHSTHYSMGANNAAALPNSS
                     RTLPILSVDSGVAFERDWSLFGHDYLHTLEPRAFYVYVPYQDQSRLPVFDTAQADFNF
                     VQMFSENRFFGSDRIGDANQITLAMTSRLLEQANGIERLKLTVGERFSFSTPQVNLVA
                     PTATTNKSDILVAASGRVTDTWSLGSEFQIDPNKSQTQRYNFASSYRPEAGKVFNLGY
                     RFFRNTLLGAPTLAAALGTQGELRLMDASAQWPLSGRWHAVGRWYYSLRDARVLEAIA
                     GLEYNESCWTLRLIAQRFTVGLQQANTGFFLQLELNDFVKVGSDPLDLLKQSVPGYKK
                     LNDKPVDNSRRALR"
     gene            16222..17343
                     /locus_tag="VFW59_00170"
     CDS             16222..17343
                     /locus_tag="VFW59_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5362662.1"
                     /translation="MQRQQELTEWLQSQFPDNGFTLAPASADASFRRYFRVSFADGRT
                     LIAMDAPPQHEDCRPFVHIAKLFEEAGTHVPHIHAQNLEQGFLLLSDLGNVTYAQALS
                     DGDIGNARKLYGAATDALIKIQLASRADELPPYDEALLRRELNLFPEWYIGKHLGVTL
                     TEKQSAKLEECFSRIVANNLAQPRVYVHRDYHSRNLMYIESPLSNSDGTTSHSTKPAS
                     VQVAGYLPQVGEEANEKANFLSSPGILDFQDAVYGPITYDLASLFKDAYIGWEENSII
                     DWLILYWEKARRAGLPVHDDFGELYRDYEWMGVQRHIKVLGIFARLSHRDGKDGYLKD
                     MPLVMKYLRAACERYIDLKPLLNLLEELEPRELQEGYSF"
     gene            17340..>17523
                     /locus_tag="VFW59_00175"
     CDS             17340..>17523
                     /locus_tag="VFW59_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5362663.1"
                     /translation="MRAMILAAGRGERMRPLTDHTPKPLLPVGGKPIIVWHIEQLVRA
                     GITELVINHAHLGHQIE"
BASE COUNT         3768 a         5580 c         4681 g         3494 t
ORIGIN      
        1 cgctcggacc agtcaccggc gctgtcctgc accgtttctt gcaccacgat ttcggtttcg
       61 cccacctctt taatcaaacc gaagttcatc ccgacatagt tacccgccct gacccggtgc
      121 aacttgccat cgggggcacg aataatggca tatccaacct tgttttgacg gagataaccg
      181 accatgtgca acccctccag aggaaactcc tccaaggcct cttttggacg atccatatcc
      241 ggctgattca agcccggacg gctcgcggaa cgcgagtcgg gcttgcgggg cttgaatgga
      301 tcctgcaagt tatccacatt gtcatagaca aagggctcgt acggcttcac ttccggagga
      361 ggtggtatct tgccgcgcaa atctgctccc gcattctgaa cgaagtcacg cagatcctga
      421 aactcttccc cggcgcatcc cgtcaagagc gccgaagcca acaacagatg catcagtttc
      481 atggtttttt cgcccccgtc gccttcttct ggctcgccac ttcctcttcg tccaaataac
      541 ggaaagtctt ggcgattgca tccatggaca gcgcagtacc caaaggactc accgatattt
      601 cattcagcgt aacgatgcgc ggcaacatgg aaatgtcgct ggaaaacttg ccgatatcat
      661 cataattacc cgtcaccttg atggtgatag gctgctcggc aaaggcgccg ttgactgttt
      721 cagcccccgg acggaacagc tcgaactgca aacccctgcc cagtccagcc tgattgatat
      781 cagtaagcaa ggcatccatt tccgacttgc tcggcaactg cttgagcaag gcgccaaaag
      841 actcttgcgt ctcggtcagc tgcttcttga tcagatccag attaattgcc tgttttttct
      901 ttaccaggaa agtatcttta agcgtttgct cttctctttt aatcgcctgc agactattcc
      961 actgctcctg ccagtcaaga aatgcgcctg cggcaaccac gctcaggaat aacacgagga
     1021 aggcgagtat cttagcgggc cacggccacg ctcccggatt cttcgggtta aggttcttca
     1081 gttcgtccag attcatcatg gctgcacctg ggtcttagtc agattaaacg tcagcgagaa
     1141 ttcgcttacc cgcgcaccat tcgcactact agcatgaatc tcaaccagag aaggggtatc
     1201 cagccaaggc gagtcttcaa tcgcacgcat caacgtggaa atacgcgcat tggactgcgc
     1261 ataaccgacc acactgattt tgttcccggt ttgacggagc gacttcagat acacgccgtc
     1321 cggcaatatg cgcaacatct gatcgagcag gtgcaccaca tcggaacgcg tgctttgcag
     1381 accttcgact acattcttgc gcgccaacag ggaatccgtc tgttcacgaa gctttctgat
     1441 ttcggcaatt tgccgatcca atgcagcagt ctcctgcttc aaatattcgt tgcgccgctc
     1501 ttgataggaa atctgggtgc cgattgcaac atgcacgaat ccgaccagca tggcgcccag
     1561 cagcactgaa ataacggcaa aagcaccgaa ctggatctga cgggcacgac gcttctcggc
     1621 gcggtgcggc agcaggttta tacgtatcat gatggatcaa acctccgcat tgccaacccg
     1681 cacgcaacaa tcaaagccgg agcatcggcc tgcaactgac gcggcttgat acgaccggac
     1741 agggtcatca aggcaaatgg attcgcgacc atcgtgctta cctgagtccg ggtcgccact
     1801 gcatcatcca gacccggcaa taccgcacag ccacccgcca gaacgatgta gtcgacttcg
     1861 ttgtattgcg tagaagtaaa gaagaactgc aaggcacggg cgatctccat caccacgctc
     1921 tcgcggaacg cagacagcac atcactctcg taattatccg gcagcccgcc atttcgcttg
     1981 gccgcctctg cgtcttcagc cgacaagccg aacacggcct gtatctgctg ggtcaattgg
     2041 tcaccaccaa cctgttgatc acgcgcataa accgactggc cattgcgcaa cacgttgaca
     2101 ctcatcacgc tggcaccgat atctaccaac gccacgcact tgtcctcagc gccatccggc
     2161 aactgcatcc gaatctgttc gaatgccatc tgggcagcgt aaggctcgac atcgataacc
     2221 accgccttca gccctgccac ctccgccgcc gccacgcgat cttccacatt cgctttccgc
     2281 gatgcagcca acagcacctc gatctcatcg ggattgcctg gcgcagggcc gaccacctga
     2341 aagtcgaggt tgacctcttc cagcgcaaac ggaatgtact gattcgcctc ggattcgact
     2401 tgatactcca aatcctcttc gcgcataccc gccggcagca gaatcttttt ggtaataacg
     2461 gctgcggcag gaagggccaa cgcgacgttc ttgatccgaa cccccattcg cttccaggct
     2521 cgctgcaaac tctccgctac cgcgtccaga ttgacgatat tgccatcact gaccgcatcc
     2581 ttgggcagcg gttcgattac gtagcgctcc acgacgtaac ccgtcttctt gggcgtctcg
     2641 ctcaactcga ccattttgac cgaggatgca ctgatatcca cgccgatcaa aggtggggta
     2701 gtagcccgca aaaaatccag cggtatgtca aaattcactt taggcatagg ccgtttaggg
     2761 agtttccgta taaagtggtt taaatcctag caataactcc aaatcgtgta aagctatttc
     2821 tgaaagaggc attaatcact gactagacta tataatcacc gctttattac tgcattgctt
     2881 ccggacaact acatgacctc gcgtcgctgg ctactcttac tcatcccgct cactctactg
     2941 ctgttgcccg cgatattgtt cggcctggca gtcacgctaa cctacccgac cctgccttcg
     3001 ctggatgcac tgaccgacta ccgccccaag ataccgttgc gagtttatag cgccgatggc
     3061 gcgttgctgg gcgagtttgg cgaggaacgt cgcgctctgg tcaacatagc cgaagttccc
     3121 gatttgatga agaacgccat cctcgccgcc gaggatgatc gcttctatca gcacggcggt
     3181 gtcgattatc tgggcgtact gcgtgcagcg gtttcaaatt tcacctccgg cggtgccaaa
     3241 cagggcgcca gtacgatcac catgcaggtt gcgcgcaatt tctttctctc gaaggaaaag
     3301 accttttcgc gcaaattcaa tgaggttctg cttgcattca aaatcgaacg caatctgagc
     3361 aaggatcgta ttctcgagct gtatttcaac cagatctatc tgggacaacg cgcctatggt
     3421 ttcgccacag ccgctcaaat ttatttcggc aaatcgctta accagctcac cgtggccgaa
     3481 gccgccatgc tcgcaggcct gcccaaggca ccctccgcat acaacccggt cgtcaacccc
     3541 aaacgcgcca agctgcgcca gctgtatgtc ctgcgccgca tgcacgaact gaatttcatc
     3601 aacgatgagc aatttcaact tgcgcaacaa caacctctcg tagccaagca cggcaatcag
     3661 gagttcggca ccaaatcaga cttcatcgcc gagatggtcc gtcaggccat ctatgagcaa
     3721 tatcacgagg atacctatac ttcaggcctc aaggtctata ccaccatacg ccagcaagat
     3781 cagcttgccg cttatcaggc cgtgcgcaag aacgcgctgg actacgacca tcgccatggc
     3841 taccgtggcc ccgagagctt cgtcgatctg agcacaggca acagcgaaga acaactggaa
     3901 gaagcgctgc aagatgtcac cgacaacgac gatctgatcc ccgccgtggt gcttgccgcc
     3961 tcacccaagc ttgtccgcgc ctattgcaag ggcggcgagg tcgccgaagt gagcggcgat
     4021 gccctggcct ttgcgcagcg ggcgctgaac ggtaaagtcg cactcaatca acgcatcatc
     4081 cctggctcga tcattcgcct gcaaaaggat gacaaaaaca actggcaaat cacccaattg
     4141 cctcaggttg aggccgcctt cgtttctgcc gacccacgta acggcgcgat cctggcgctg
     4201 gtcggtggtt ttgatttcaa tcacaatcaa tacaatcacg tcactcaggc atggcgtcag
     4261 cccggctcca gcatcaagcc cttcatctat tcggcggcat tggagaaggg ctttacgccg
     4321 gcgacactga tcaacgacgc gcccttgctg tttgacgcca cgcagacagg aagcgatccc
     4381 tggcaaccca agaattatga cggcaagttt gaaggcccga tgcgcatgcg cgaggcgctg
     4441 accaagtcga agaacatggt gtcgatccgc atcctgcaat ccatcacccc gcaatatgcg
     4501 caagactacc tgaccaaatt cggtttcgat gccgacaagc acccgcctta cctgactatg
     4561 gcactgggtg ccggcatggt caccccgatg caaatgctcg gcggctattc ggtgttcgcc
     4621 aatggcggct tccgcatcca gccctacttc atctcgcgta tcgaagatgc caatggcaag
     4681 gtgctcagcc gtgcaacacc cgtcgttgcc gccgagaatg cagaacaggt catcgatgtg
     4741 cgcaacgcct atacgatggt cagcatgatg caggatgtcg tccgacgcgg cacagcaacc
     4801 gctgcaatgc gtttgggtcg ttcggatctg gctggcaaga ccggcaccac cagcgactcg
     4861 atcgacgcct ggttctgcgg cttccagccg actgtagtag gcatctcctg gatgggcttc
     4921 gatcagccac gcagcctggg tgatcacgaa actggcggcg gcgcggcgct gccgatgtgg
     4981 atcgcctata tggaaaaggt gctcaaggat gtgccgcaag ccgtttatga gatgcctgag
     5041 aatatggctg ccgtgcgcat caatgcgtcg gggcatcgcg atgaaaatgg cagcctgatc
     5101 gattatttct atcaggaaaa tatcccctcc gaacaggcgg cccctccccc acctctccct
     5161 ggcagcgcag tcaagccgac cgagaccctc aaagatcagc tgctctgaac cgatgaacaa
     5221 ggaacatgga caccggcgcg atctgatgcg cgaacagatc gcgcatcaag ccgcaaagtt
     5281 gatggccgag ggcagcatca ccgaccacgc gttcgccaaa cgcaaagccg ccaaacagct
     5341 tggcgccgca gacacccagc acctgcccag caatctggaa gtagacgacg cgctgcacag
     5401 ctaccgcgcc ctgtatcaac gggacagcca tccggccatc ctgcgccagt tgcgcgatga
     5461 agccctgagc acgatgcgac agttagccgt cttccacccc tacctgaccg gctcggtgct
     5521 gaccggaacg gcaggcgtac aatccgacat caacctgatg ctgttttctg acgacgagaa
     5581 ggcagtgctg ctgtttctgc tcaaacacaa actcgagttt gaggacggag aatggcgcgt
     5641 ccgcatagcc gggcgtgaag aatcggtgcc gagctacaca ttgaccgccg aatcaggtat
     5701 ccagacgcac gtcatcgtac tgccggagaa cgcacgacac agcggcagcc gccacccgga
     5761 gacccatgcc gatatctcag ctgtagaggc tttgctggcg ggttgatgac ggattgagca
     5821 gagcctcgta cgaccaggct ccgcaaagac aaagaccggg aatcgcgttg ctcatcttgc
     5881 gcgaacccaa ttccgaggaa aaacagccat ctgactaggc tgtcaaccct gcttcagcca
     5941 cttcgccgca tccagcgcga aataggtcag gataccatcc gctcccgcgc gcttgaatgc
     6001 cagcaaggac tccagcacac aggcccgttc atccagccag ccgttctgcg cggcagcctt
     6061 gagcatggcg tattcgccgc tgacctgata gacgaaagtc ggcgccttga attcatcctt
     6121 cacgcggcgc aggatgtcga ggtagggcat accgggcttg accatcacca tgtccgcgcc
     6181 ttcctgcaag tcgaggccga cttcccacag cgcctcgtcg gagttcgcag gatccatctg
     6241 gtaggtgtat ttgttgccct tgcccaggtt gccgctggag ccgacggcgt cgcggaacgg
     6301 gccgtagaag ctggaggcgt atttcgccga gtaggccatg atgcgggtgt ggatgtgtcc
     6361 gttggtatcc agcgcggcgc gcaccgcgcc gatgcgcccg tccatcatgt cggaaggggc
     6421 gacgatatca gcgcccgctg cggcgtggca ctgcgcctgt ttagtcagca ccgcgatggt
     6481 ctcgtcgttg agcacatagc cgctgtcgtc gatcagtccg tcctggccgt ggctggtata
     6541 ggggtcgagg gcgatgtcgg tcatcacgcc gagctcgggg aaatgcttct tcagctcggc
     6601 cacgacgcgc ggcaccaggc cgtcagggtt ataggcttcg cgcgcatcca gacttttctg
     6661 cgccacatcg atcaccggga aaatcgccat cacgggaacg cccagcttga cgcattgctc
     6721 ggcatccttg agcagcaagt ccagcgtttt gcgctgcacg cccggcatcg acttcacatc
     6781 ctctaccttg ttcgcgcctt ccagcacaaa aaccgggtag atgaagtctg ccgatgtaag
     6841 cacatgctct cgcatcagat cgcgagaaaa ctgatcatgg cgcatccggc gcatgcgttt
     6901 atgtgggtac tgtccgagtg tctgcatgat tttccctgaa aaattatgtt ttctttggaa
     6961 ctaattaaga aaccgcttat ctgaacgtgt gaatgatgat gaatcgttcc acgtttctcc
     7021 tccctgagcg tgtaatctta accttgttaa gatgtttgcc cccggtacgc cgggggcatt
     7081 tttttagcca gaattcgagt ggccaatctc acctagtccg gctcaaccgg gctaacctct
     7141 ggcgcgagcc agcccgcgat caccttctcc gcctcttcga taccctgctt cttcaagctg
     7201 gagaacagtt gcaccgaaca gttcggaaag tgctccttga ggtaggcctt cacttccatc
     7261 aatgtctttg tcgcctgctg gcggctgagc ttgtccgact tggtcagcag cacatgcacc
     7321 ggcttgccgg tcggcgcaaa ccagtccagc atgccctcgt ccagcgccgt cagcggatgg
     7381 cgcgaatcca tgatgacgac cagcccggcc aactcatcac gagtttgcag gtactgaccc
     7441 agcagctttt cccaatgctt ctgcacatcg ggcggcacct tggcgtagcc gtaacccggc
     7501 aaatccacca aatacttgcc ttcagccagc gaaaaatagt tcagatgctg ggtgcgccct
     7561 ggcgtcttgc tggtgtaggc cagacgcaca tggttcgcca gcgtattgat cgcgctcgac
     7621 ttgcccgcat tggaacgtcc gacgaacgcg acttccttgc cgccatgcag cggcaagtct
     7681 ttcatgtggt tgacggtggt gtagaaggcc gcctgagaaa agagtcccat tacttccagg
     7741 ccgcaaagac tttgtcggcg gcagcaatgg tcgccgcgat gtccgcctcg gtatgcgcgg
     7801 cggacacgaa gcccgcctcg aatgccgaag gggcgaggta aacaccctcg tccagcatcg
     7861 catggaagaa gcggttgaat gccgccttgt cgctggccat cacctccgca tagctggtcg
     7921 gcacatgcgc gctgaaatac aggccgaaca tgctgccgat ggactgcgcg ctgaatggga
     7981 tgccgtgctt cgcggcactg gcgctcaagc cttccgtcag ctgtttggct acctgtgtca
     8041 ggtgctcgta gaagcccgga gcctgtacca gcttgagcgt agtcaaacct gccgccaccg
     8101 cgaccggatt gccagacaag gtgcccgcct gatacaccgc acccagcggc gccagacatt
     8161 gcatgatgtc gcggcgaccg ccgaaggctg cggcgggcaa gccgccgccc atcaccttgc
     8221 ccagcgtgat cagatccggc ttgatgccgt agtagccatg cgcgccctgc aggccaacgc
     8281 ggaaaccgga catcacttcg tccagaatca gcaccgcgcc gtgtttcgtg cacagcttgc
     8341 gcatcgcatc gaggaactcg cgctttggca gaatcagatt catgttgccg gcaaccggct
     8401 cgacgatcac cgcagcgatc tcgttgccgt attcggcaaa ggccttttcc agtccggcgg
     8461 catcgttgta atccagcacc gtggtcagcg cggcgatctc ggcgggtacg ccggaagagc
     8521 tcggctgacc gaaagtcagt gcgccggagc cagccttgac cagcaggccg tcggaatggc
     8581 cgtgataaca gccttcgaac ttgataatgc ggctgcgccc ggtgaagccg cgcgccagac
     8641 ggatcgccgt catggtcgcc tcggtaccgg agctgaccag acgcaccatt tccagagacg
     8701 gcagaatctt gcacagcagt tcggcgatct ccagttcggc tgccgtcggc gcgccgaatc
     8761 ccagaccttc cgcagcggcg gcttgcacgg cctttaccac ctcgggatga caatgaccga
     8821 cgatcatcgg cccccaggaa ttgacgtaat cgacgtactg cttgtcatcg gcatcccaaa
     8881 cataggcgcc cttgccgcgc ttgaagaaca gcggcgtgcc gcccaccgac ttgaaggcac
     8941 gcaccggcga gttcacgccg ccggggatga tcttctgaga ttcgtcgaac agctgttggt
     9001 tatgcgaagt catgttgcat ctttctgcct gatatttaac gggaactact caaattcaaa
     9061 tctgcgaact gacgcgccgc ctcctgcaca tccggcgcgg cgaatacggc cgagatcacg
     9121 gccagcgcat ccgcaccggc tcgcaacaac tgcgcaccgt tctgcaccgt gatcccgccg
     9181 atcgcgacga tgggcacatg cagctcctga cgcgcctgct gcaacaaccc gagcggcgcc
     9241 tgcaccgcag caggctttac gccggaagcg aagaacgcac cgaacgccac ataatctgcg
     9301 ccctgccgca ccgcctcatg cgccagcggg aggcgattat aacaagacac gccgatgatc
     9361 ctgccactac ccagcagcgc acgagcctct gcgaccgatc catcatcgcc ccccagatgc
     9421 acgccatccg cctcgacctg acgcgccagc accgcgtcgt cattgatgat caatggcacg
     9481 ccaaactcac gggtcaagcg gcgcaatgcg cccgcctgtt cggcgcgcaa gggaagaggt
     9541 gccagcttgt tgcggtattg cagcaggtgc gcgccgccca gcagcgccaa tcgcaccttg
     9601 tacaacaatg ccgccgtatc cagctcgtcc ggcgtgatcg cgtaaagccc gccgattacg
     9661 cgctgaacgg cgcgatgagt ctcaacctcg ctcacttgcc accccctcca caatcctccc
     9721 tccgctcgcg ggagaggagg caatcgtccg ccccgaaggg cgaaaacctt ttgcccctgc
     9781 cgctaattcg ggagatcggg tgcatcctct tcgccgcgcg cccagaacag acgatccgga
     9841 atatgctgcc ccatgccggg acggaagcct gcgctcagcg tctgccaagt atattcctgc
     9901 gcctccttca ccgcctccgg cacatccaca tcctgcgcca gcaaggccgc gatcgcggaa
     9961 gccagcgtgc agcccgagcc gtgatagatg cccggcaggc gcgcccaact ctcgctgctc
    10021 acgatgccgc gctcgctata cagcgtattg accaccttgg ccgtctgctc atgggtgccc
    10081 gtgatcagca catattcgca gcccatggcc aacaaacgct tcgcgcactc atccagactc
    10141 gggtcatcat cttcgttatc ttcatccagc gcgagacggc gagcctccat gctgttcggc
    10201 gtgatgatgg tcgcttgcgg aatcaataac tcgcgcatcg catccagcat atcctcgtcc
    10261 gccaactcgt cgccacgccc ggaggccagc accggatcaa tgaccagcgg cacatcggga
    10321 taatcggcca gcacctcggc gatcgccgcg atgttttcca cgctgcctag caggccgatc
    10381 ttgaaggcgg ccaccggcac atcttccagc atcgcgcgcg cctgatcctc cacccattcg
    10441 gaatcgatcg gcaggatgtc atccacccca ctggtgtcct gcaccgtgat ggcggtgacc
    10501 accgacaacg gatggcaacc catgctggcg atggtcaaca ggtctgcctg catccccgca
    10561 ccgccactgg ggtcggttgc ggcgaaagtc ataactaatg gaaaggattc ggacatgatg
    10621 tggacgatgg tgcgttcggt cgggcatttt accctatatg tccatcgtgt cttaattcaa
    10681 acgcaaacac atcggccgaa cagccctggc ggtggattgc actaatccag ccctttcccg
    10741 tcgagataat tcgccacctt gataaagtcg tccaccgaaa gattctcggc gcgcaattgg
    10801 gagttgatgc caagccgggc gaaatcctct tcgttcagat gatcgcgcag cgtgttgcgc
    10861 agggtcttgc ggcgctgacc gaaagccgca ctgacgatct gcgcgtacag cttctcgttc
    10921 ttcaccgcga tatcggcccg tggcagcggg atcatgcgca cgatagcgga atccactttg
    10981 ggcgcgggac gaaaggattc cggcggcacg tcgagcagtt tctccatgta gaagcggtat
    11041 tgcagcatca ccgacaaacg cccataggcc ggcgtcgaat gctcggcgac catgcgctcg
    11101 accacctcgt tttgcagcat gaaatgcata tccttcacgc gctcggtgta agcggcgaag
    11161 tggaacaaca gcggcgatga gatgttgtac ggcaggttcc cgacgatgcg cagcggactg
    11221 ggcaaggtcg caaaatcgaa ctgcagcgcg tctccggcat gaatggtcag ctttctgctg
    11281 gcggcctcct gcggatactc atgctccagc cgcgcgatga tgtcgcggtc gatctccacc
    11341 acatgcagat gattcaaacg cttcagcaag gggcgcgtca gcgcacccag ccccggcccg
    11401 atctcgacca tgttgtcgtc cggctcggga cgtatcccgg cgacgatgtc ggcgatgatc
    11461 cgctcatcga ccagaaaatt ctggccaaat ttctttttag ctttatgact gctcatgcgg
    11521 atgcccaaac cagaattgct catattgact catttctgca gcaagcttga tcgcctccag
    11581 cagactgccg acctcggccc tgcctgttcc ggccagatcc agcgccgtgc cgtgatcgac
    11641 cgaggtgcga atgatcggca ggccgagcgt gacattcaca ccctgaccga aactggcatg
    11701 tttcagcaca ggcaacccct gatcgtggta catcgccagc acgcagtcgc attgcgccag
    11761 tttgcgcggc gtgaacagcg tgtcggcagg caagggggcg ctgacctcaa aaccctcggc
    11821 gcgcaacttg tcgagcaccg ggatcatcac atcgatctct tcacgcccca agtagccgcc
    11881 ttcgcccgca tgaggattca gccctgccac cagaatgcgc ggtttcgcga tgccgaagcg
    11941 gcgctgcaaa tcgcactgaa tgatgcgcaa tactttttcg agcaacgctg cggtaatcgc
    12001 gtcgggaacg tcccggagcg cgagatgggt ggtcgccagc gcgacgcgca tgccgccgcc
    12061 gaccagcatc atcaccacca gcggcgtacc ggtctgttcg gcaagatatt cggtgtgacc
    12121 ggtaaacggg atgccggcat cgttgataat gcctttgtgc accggtgcgg tgaccatgcc
    12181 ggagaattcg ccagactgac acccctggag cgcacgacgc agcgtcgcca gcacatagtc
    12241 gccgttcgcc gcatccagca cccctgcctg acagggtgcc gcgagcggca cgtgaatgac
    12301 agatagagag tgggaagtag ctgacgcccc cctactcacc actttccact caccactcac
    12361 catttcgcgc agttgcagcg aaatgccgag ccgttcggcg cgctgttgca acagatattt
    12421 atcggcgatg acgacgaatg gaatatccgg cgcggcgacg gccagctgca cacacaggtc
    12481 ggggccgatg cccgccggct ctccggcggt gatcgccagc ggcgcggcaa ggaagggggg
    12541 actcacctgt tctcttcgag gcgatattcg atgaaggcgc gatcacgcaa ctggcgcagc
    12601 cagtcctgat attgctcatc cgacttgaac gtaccgagtg ccatacgtgc ctgttgacgt
    12661 ttttgttgca cgctgacatc ggtattgcgc cgctccagca cttggatcaa atgccagccg
    12721 aactgcgatt gagcgacacc gaccataccc ggctgcaacc tgttcatcgc cgtttcaaac
    12781 tccggcacag tctgcccggg cgacagccaa tccagatcac cgccctgctg ggcgctgcca
    12841 tcctgcgaat aacgcttggc ctgctccgca aaatccgcgc ccgcctcgat gcgtgccttg
    12901 atctccatca gacgtttctt cgcctcattg tccgagacga tctcgctggt cttgatcaga
    12961 atatgacgcg catgggtctg agtgatcacc acttgcgcga cgccgctacg cttctcgatc
    13021 agcttgagaa tatggaaacc gctggaacta cgcagcaccg ccgtcgtttc gccgggctgc
    13081 aagccctgca acgcctccag aagcaccggc gggatgcgag tcgcaggacg ccagccgagg
    13141 tcgccgccac tcaatgagtc cttcgcatcc gaagcacctg cggcgacttg cgcaaaatcc
    13201 gcgccgcctt tcaattgagc cagcgcctgc tccgccttct cgcgtgccgc ctgaatcctg
    13261 tcggcgctag cctgctcggg caccaccacc agaatatgcg ccagatgcag ctcgtcctcg
    13321 gccccgccca tcttggcctt gttggccaga taactatcga cctcgctatc gctgatcacg
    13381 accttgctct cgacttcgcg ttcgcgcagc cgggtggaga tgatctcgcc acgaatctcc
    13441 tcgcgaaact tgttgaaatc cacaccctcc gattccaact tggcacgaaa ctccgcaagc
    13501 gacgtaaaat tattctgctg agcgatgcgg gtaagcgaca gattcagctg cgtatcgtcc
    13561 acgcggatgc cgctctcctt ggcaaactgc gcctgcagca ggtcggtgat catgcgttcg
    13621 agtatctgtt tctccagcag atccggcgcg ggcaacggcg tgccctgctt ctgcaactgg
    13681 cgcaccacca ccgccagctt gtcatccagc tcgtggcggg tgatcacatc atcgttcacc
    13741 acggccacca cggcatcgat caccgcgact tgtttctgcg ggtcggatgc tacagccggt
    13801 gcaacaaccg gcatatcggc ggcatgagcc aacggccaca ccagcatggc agcacacatc
    13861 atcaggcatt tcattcgcat catgtttctt tccatagtca gcgcaaagcg cgtcgagagt
    13921 tatcgaccgg cttgtcgttc agttttttat agcctggcac gctctgtttt aacaagtcca
    13981 gaggatcgga gccgaccttg acgaaatcat tcaactccag ctgcaggaag aaaccggtat
    14041 tggcctgctg caagccaacg gtaaagcgct gcgcgatcag gcgcaatgtc cagcagcttt
    14101 cattatactc aagcccggcg atcgcctcca gaacacgcgc atcccgcaac gaatagtacc
    14161 agcgccccac cgcatgccag cgaccggaca gcggccattg cgccgaggcg tccatcaaac
    14221 gcaactcacc ctgagtgccc agcgcagcag cgagcgtcgg cgcaccaagc aaggtgttgc
    14281 ggaagaatcg ataacccaga ttgaacacct tgccagcctc cgggcgataa ctcgacgcaa
    14341 agttataacg ctgggtctgc gatttgttcg gatcgatctg gaactcactg cccagcgacc
    14401 aggtatcggt gacgcgcccg ctggccgcca ccaggatgtc ggacttgttg gttgttgcag
    14461 tcggcgcgac cagattgacc tgcggagtcg agaaactgaa acgttccccc accgtcaact
    14521 tcaatcgctc tatgccattc gcctgctcca gcaggcgcga agtcatcgcc agcgttattt
    14581 gattggcgtc gccgatccgg tcactgccga agaagcggtt ctcggagaac atctggacga
    14641 agttgaaatc ggcctgcgcc gtatcgaata ccggcaggcg gctctgatcc tgatagggaa
    14701 catacacgta gaaggcgcga ggctccagcg tatgcagata atcatgaccg aacagactcc
    14761 agtcgcgctc aaacgcgacg ccgctatcca cactcaagat cggcagcgta cgcgaactat
    14821 tcggcaacgc ggctgcatta ttcgccccca tcgaataatg cgtgctgtga agcgccgctc
    14881 tcggggtcag ataaaatgcc tgattgctga tcagcggata actcacggca gggttcagca
    14941 ccagccgctt gccattcact gccgtcggat gggcgaaatc gacgaattcg ctggcaaagg
    15001 acacgctggc gccggaatag ttctgggcag cgttgaaggt caactgcggc agtcgcttgt
    15061 aaggcacggc taccggcgcg gccggatctt gcagggtctg atagcgctgc acacgcatgg
    15121 cggcattcca cggcccggcg ccgtagctca gcaccccctc ctgcatcaga ttgatctgag
    15181 aagtgacatt caccgaactg gccagatcac ggtaatagtt attgtccgaa acgctattca
    15241 gattgaggta gccattcagc ccgccgtaaa tgttctgacc atgcaaaaca ccgaggcgag
    15301 tgcgcgtgct tttcgtcagc atgtcgctcg gcagcacatc cacgtgcaac tcaccggagt
    15361 agccgggttc cagatagcgg aattcgttgt tgaattgcac cccgcgcttg gccatcacgc
    15421 gcggcgcgat ggtcgcatcc aggttctgcg cgatattcca gtaataaggt accgtcagtt
    15481 cgctgccacc cttggtggtg ctgccgatga tcggcgccag aaaacccgac ttgcgttgat
    15541 cgttgagcgg gaaatccatc cacggcgaat acaggatggg cacccccatg aactgcaccc
    15601 aggcattgtg tgccgtgccg atctgccggt cacgatccag ctccagcccg ctcatgtgca
    15661 gcaaccagtc ctcgttgtcg gccgggcagg tggtataggt cgcgttatcc agctggtagt
    15721 gcaacttgtc ctgaaagtgc atcacatcag ccgatccgcg tccgtcgttc tcgatcagat
    15781 agaagaccgg ctgctccatc accccgatgt cggtatcgag attgagcttg agatgagggc
    15841 cgctcatggt attgccgccc tgatcgacca ccaccgaacc ttgcgcgtcg agatcgctcg
    15901 tgttctgaaa atacaacaac cgatccgcac tgatcgactg accgcgcttg cgcaacaccg
    15961 cattgccggt ggcctcgacc tgatcgtcct gcttgcccac cagccgatcg gcctccagaa
    16021 aactcggcgt ctgctgctca ggctctttca tcgccatgaa ggtcttgtcc atccgcaacg
    16081 ccagcaggtc ggcattcgca gccgcatctt gcgcgaacgc gcagagcagg gagaggacga
    16141 caggcttgag acggaaccgc ataacgatgg ccaaatggta aacttgcgcg agtttaccac
    16201 caagaccagc caaaatcaga tatgcaacgc cagcaagaac taaccgaatg gctgcaaagc
    16261 cagtttccag acaacggctt caccctcgcc ccagcctccg ccgacgccag cttccgccgc
    16321 tatttccgcg tctcatttgc ggacggacgc acgctgatcg cgatggatgc gccgccgcaa
    16381 cacgaggact gccgcccctt cgtgcacatc gccaaactgt tcgaggaggc cggcacccat
    16441 gtgccgcaca tccacgcaca gaatctggag cagggcttcc tgctgctgtc cgatctcggc
    16501 aacgtcacct acgcgcaggc cctctccgac ggcgatatcg gcaatgcccg caagctctat
    16561 ggcgcagcga ccgacgcact gatcaagata caactcgcct cgcgtgccga tgaattgccg
    16621 ccgtacgacg aagccctgct gcgccgcgaa ctgaatctgt ttccggagtg gtacatcggc
    16681 aaacatctcg gcgtcacgct gaccgagaaa cagtccgcca agctggaaga atgtttcagc
    16741 cgcatcgtcg ccaacaacct cgcgcaaccc agagtgtacg tgcatcgcga ctaccattcg
    16801 cgcaacctga tgtatatcga atcccctctc tctaactctg atggaacaac tagccattcg
    16861 actaagcccg caagcgtgca agtcgctggt tatctcccgc aggtgggaga ggaagcgaac
    16921 gagaaagcca actttttatc ctcgccaggc atcctggact ttcaggatgc cgtctacggc
    16981 ccgatcacct acgatctggc ctcgctattc aaggacgcct acatcggctg ggaagaaaac
    17041 agcatcatcg actggctgat cctctactgg gagaaggcgc gccgtgccgg cctgccggtg
    17101 catgacgact tcggcgagct ctatcgcgac tacgaatgga tgggcgtgca gcgtcacatc
    17161 aaggtgctgg gcatcttcgc ccgcctgagc caccgcgacg gcaaggacgg ctatctcaag
    17221 gacatgccgc tggtgatgaa atacctgcgc gccgcctgcg aacgctacat cgacctcaag
    17281 ccgctgctga acctgctgga agaactggag ccgcgcgaac tgcaggaagg gtacagcttt
    17341 tgagagcgat gatcctggcc gccggtcgcg gcgagcgcat gcgcccgctc accgaccata
    17401 cgcccaaacc actgcttcct gtcggtggca aacccatcat cgtctggcac atcgagcaac
    17461 tggtgcgcgc cgggatcacc gaactggtga tcaaccatgc ccatctcggc catcagatcg
    17521 aag
//
LOCUS       DASUIV010000006         5266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_280025, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000006 DASUIV010000000
VERSION     DASUIV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280025"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFW59_00180"
     CDS             <1..556
                     /locus_tag="VFW59_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5362664.1"
                     /translation="DIRRDALVGAARLIDAVSNKARQMMNGPHYIVATIGHLVLTPNA
                     SNAVPGRVEMMLEVRSDSNELLETFPQQVVESVRPEVEALRLTLIAKQVSRSQPTNCT
                     PFMIELIEGSAQALDYRSMRMPSGAGHDAVYVSKTGPMGMIFIPCLQGRSHCPEEWIE
                     PRQLLDGTRVLYETIVRLDQALPR"
     gene            761..973
                     /locus_tag="VFW59_00185"
                     /pseudo
     CDS             761..973
                     /locus_tag="VFW59_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1179..2153
                     /locus_tag="VFW59_00190"
     CDS             1179..2153
                     /locus_tag="VFW59_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5362665.1"
                     /translation="MTNCKKLRYVVATFLLCTVVAAIAQPYPAKPIRLIAPSTPGDAP
                     DVIARLIALRLSPVLGQPIVVENRPGAGGVIGSEVAAKSAPDGYTLIMGNAGSHGINA
                     AVYSKLPYDIQRDFAPISQVAIAPNIMVINPSIPAKSVAEFIAYAKSQPGKLSYASGG
                     NGSSAHMSMELFKAMSGVDIQHVPYKGSSPALTDLMGGQVAVFIGNIPPTVPLVRAGK
                     LRALAVTTKSRSTLMPELPTISEAGLSGYETVAWFGVLAPAGTPPEIVKTLSTEISKI
                     ARSPEMRDKLLAMGAEPVGGTPEEFKAVIDSDIAKWKRLAQKESIKVD"
     gene            2167..3330
                     /locus_tag="VFW59_00195"
     CDS             2167..3330
                     /locus_tag="VFW59_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5362666.1"
                     /translation="MTTISALEPVVVNISPKTNWSFIAVTTDDGATGWGECSLNGWEP
                     LLIAQAEMLAHTARGRDIEEPGSLVSYLPHTPGGLIAHAVKSATEQALVDLRARCAGK
                     SIAAYLSATPRNSVPAYANINRGVIDRSPVGFAAGAKRAVAAGYRAVKLAPFDGVIAQ
                     DAAHTPIEKLIHAGLDRIFAVRDAVGDKIDVMVDCHWRFDQSRAETLLHDVAPARPYW
                     MECMTSEHMSCFPAIARLTELAHERGTRTAGGEMIAGADVAEAMCTQRLYDVLMPDIK
                     YAGGFTGMLAIARVCGEHGIAFAPHNPTGPIAHVASIQVCAAAPTLLWLEHQWDESPL
                     FDDLIGGNKAPLVDGAFSVPTCPGLGAEVIRTLASGLPYRMLPSGVGLDERLG"
     gene            complement(3395..4072)
                     /locus_tag="VFW59_00200"
     CDS             complement(3395..4072)
                     /locus_tag="VFW59_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX5362667.1"
                     /translation="MSSNQAIDPSANDLTERLQRCYTGVVHDIMRAMGLRDFTLPPEL
                     RPIIPEQAIAGPAFTIRGKVSTGADAHETLLAWTGLLSKAPSGSIWTSQPNDRVVAHM
                     GELSAETLKNKGVRGCVVDGFVRDTNFLLDIGFQAWCRGFTPRDVVGHWLPDAINVDI
                     EIGDVRIAPGDYLVGDRDGLIRIPQRYLEEVVQLSEKAIATESLVRSAILQGVDPQQA
                     YLKYGKF"
     gene            complement(4199..4405)
                     /locus_tag="VFW59_00205"
     CDS             complement(4199..4405)
                     /locus_tag="VFW59_00205"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5362668.1"
                     /translation="MQLLHRAHPCNSHFRTLAEQVKIGMYEAGGFPLEFPVMSLGEFL
                     LRPTVMLYRNLASMDVEESIRGNA"
     gene            4658..>5266
                     /locus_tag="VFW59_00210"
     CDS             4658..>5266
                     /locus_tag="VFW59_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5362669.1"
                     /translation="MSRPHILTVAKLPPFLMEPLNAAFVVHDRLHESDPAAFTKVAPE
                     IRGIAGGGESKVPRSLMDQLPALEIVSIMGVGYDGVDVPAALERHVPVTHTPGVLTDE
                     VADLAIGLMLSVARRIPQADQYVRTGRWARDGAMPLTRKVSGARLGIVGLGRIGQAIA
                     SRAEAFGMSIAYTARSAKPGLSYNFYPTAQALAAQVDFLVVIT"
BASE COUNT         1129 a         1507 c         1535 g         1095 t
ORIGIN      
        1 ggacattcgt cgcgatgcac tggtgggtgc cgcgcgtctt attgatgctg tcagcaacaa
       61 ggcccggcag atgatgaacg ggccccacta tattgtggcg acgatcggcc acctggtact
      121 caccccgaac gcctcaaatg cggtccccgg gcgggttgaa atgatgctgg aggtgcgcag
      181 cgacagcaat gaattgctgg agacttttcc gcagcaggtt gtcgagagcg tccgtcctga
      241 agtcgaggcc ctgcggctta ccctgatagc caagcaagtc agccgatcac agccaaccaa
      301 ttgcacgccc ttcatgattg agttgatcga aggttccgcg caggcgctcg actatcgttc
      361 catgcgaatg ccaagtggtg ctggccatga tgccgtgtat gtctcgaaga cggggccgat
      421 ggggatgatt ttcataccct gcctgcaagg acgcagccac tgcccggagg agtggattga
      481 gccccggcaa ctgctcgatg gcacacgcgt gttgtatgaa accatcgtgc gactagatca
      541 ggcgttgcca cgatagagat cccatgaact ggcaactgca caggcaaaca cttagtgccg
      601 gtcatagtgc tcaacgcggc acgattgccg tcaccgtcat caagcgttcc aacatccatt
      661 ttctggaaaa tgaaagtgtc gaaatcggag gggtgcgctt cctgaagacc accctctgga
      721 cggactaccg gttggagatt gaccacatga cctgcctgat ctcaacaaca tcgtggaaca
      781 gtaccatcgg gccatcaaac gcgtcactag gcctatgctc aacttcaagt catttcaaac
      841 agctaaaagc gtcctggctg gcatcgaact catgcacatg attcgcaaag gacagctcat
      901 gatggaaggc tgcaaagagg agtcttttgc cgagcagttt tatgcattga cagggaaaat
      961 ctccgctcat tagggatcca gaggaagtct tggagcaggt ctggggcatc aggctccagc
     1021 gacacccgtt cagcaacccg gcatccgttt tgagccctga gatgttattc tgtcgccatc
     1081 ggccttgtgt caccgttcag ccgtgttcgt ttttagtggc tccagtgagc ctcgcgtggc
     1141 attgccatcg cgaccgtaat ttacaaaggg agccaaacat gacaaattgc aaaaaattgc
     1201 gctacgtcgt cgccactttc ttgctctgca cggtggtggc tgccatcgcc cagccttatc
     1261 cggccaagcc catccggcta atcgcaccaa gtactccggg agatgcacca gacgtgattg
     1321 cccggttgat agccctaaga ctgtcgccgg tgctcggcca gccgattgtg gtcgagaacc
     1381 gccctggtgc gggcggcgtc ataggatcgg aggtggctgc caaatccgcg cccgatggtt
     1441 atacgctcat catgggtaat gccgggtcgc atggcattaa tgcggccgtg tattcgaagc
     1501 ttccctacga catccagcga gattttgcgc cgattagcca agtggcgatt gcgccgaata
     1561 tcatggttat caatccatca atccccgcca agtcggtcgc cgaattcatc gcctacgcga
     1621 aaagccagcc ggggaaactg tcgtacgcgt cgggcggcaa cggcagtagc gcgcacatgt
     1681 caatggaatt gttcaaggcg atgtcggggg tggacatcca acacgtgccg tacaagggat
     1741 catcgccggc gctcacagac ctcatgggag gacaggtcgc ggttttcatc ggcaacatac
     1801 cgccgaccgt gccgcttgtg agggcgggaa aactacgcgc gctcgccgtg acaaccaaat
     1861 cgcgttcgac gctgatgccg gaactgccga cgatcagcga ggccggcttg tccggatacg
     1921 aaactgtcgc atggtttggc gtactggctc ccgcaggtac tccaccggag attgtgaaga
     1981 cactgtcgac cgagatcagc aagatcgcgc gttcgcccga gatgcgcgac aagttgttgg
     2041 cgatgggcgc cgaaccggtt ggtggtacgc ctgaggagtt caaagcggtc atcgacagcg
     2101 atatcgcgaa gtggaaacga cttgcacaaa aagagagcat caaagttgat tgaagcacgt
     2161 ggtcgcatga cgacgatttc cgccctggaa ccggtagttg tcaacatctc acccaagacg
     2221 aactggtcgt ttattgccgt cacaactgac gacggtgcaa ccggctgggg cgagtgttcg
     2281 ctaaacggtt gggaaccgct gctcatcgcg caggcagaga tgcttgctca cacggcgcgg
     2341 ggccgcgaca ttgaggagcc gggcagtctc gtcagttatc tgccgcatac gccgggaggc
     2401 ttgattgcgc atgcggtgaa gagcgccacg gaacaggctc tcgtggattt acgcgctcgc
     2461 tgcgccggga agtcaatcgc ggcctacctg agcgcaacgc cacgcaacag cgtacccgcc
     2521 tacgcgaata ttaatcgcgg cgtcattgac cgctcgcccg tcgggttcgc cgccggcgca
     2581 aagcgcgcag tggccgcagg atatcgcgca gtgaagctcg cgccattcga tggcgtgatt
     2641 gctcaagatg ccgcgcacac gccgatcgag aagcttattc atgccgggct ggatcgtatc
     2701 ttcgctgtgc gcgacgcggt cggcgacaag atagacgtca tggtcgattg tcattggcgc
     2761 ttcgaccaaa gtcgcgccga gacgctgttg catgacgtcg ccccggctcg tccatattgg
     2821 atggagtgca tgacctcaga gcacatgtcc tgctttccgg ccatcgctcg cctgaccgaa
     2881 ctcgcccacg agcgtggaac ccgcaccgcc ggcggcgaga tgattgcagg tgcggacgtg
     2941 gccgaagcga tgtgcacgca acggctctac gatgtgctga tgccggacat caagtacgcc
     3001 ggcggattta ccggcatgct cgcgatcgct cgcgtctgcg gcgaacacgg catcgcgttc
     3061 gcgccgcaca atcccaccgg cccgatcgcc catgtcgcca gcatccaggt gtgcgccgcc
     3121 gcgcccacac tgctctggct cgaacatcag tgggatgagt caccgctttt cgacgacctg
     3181 atcggcggca acaaagcacc gttggtggac ggggcgttct ctgtgccgac atgccctgga
     3241 ttgggggcgg aggtcatccg tacgctggcc agcgggcttc cgtatcgaat gttgccaagc
     3301 ggtgtcgggc tcgacgagcg cttgggataa gaaatcgaca ggatgttcaa gctcacggat
     3361 caaattattg ccaaagaatt ttctaatggg ttcatcaaaa tttcccatat ttcaaatacg
     3421 cctgctgcgg atcgaccccc tgaagaatgg cgctacgaac caaactttct gtggctatgg
     3481 ctttctcaga caattggaca acttcttcca aatagcgttg tggaattcga atcaaaccgt
     3541 cacggtcgcc aaccagatag tccccgggtg caattcgaac atcgccaatc tcaatatcaa
     3601 cgttaatagc atcaggtagc cagtgtccca ccacatcacg tggcgtaaac cctcggcacc
     3661 acgcttgaaa accaatgtcc agcaagaaat tggtgtcacg gacaaagcca tctacaacgc
     3721 agccgcgaac acccttgttc ttaagagttt cagcggaaag ttcacccata tgcgccacca
     3781 cgcggtcgtt aggttgtgat gtccagatgg aaccacttgg cgccttggaa agcagaccag
     3841 tccacgcgag tagtgtttca tgggcatctg cgccggtaga gaccttgccg cgtatggtga
     3901 acgctggccc cgcaatggcc tgttcgggaa tgatgggacg taattcaggc ggaagggtaa
     3961 agtcacgcag tcccatggca cgcatgatgt catgcaccac cccggtatag cagcgctgca
     4021 agcgctcggt caaatcgttt gcactgggat cgattgcctg attggaagac ataagggtcc
     4081 tatcggagta aagtttttgt aggtcaggat gaactcttgg tggcccattt cttctgactt
     4141 gctgggctcg ccctgcgcaa cgcgaactac aagctgagcg atttcttgcc cgacttcttc
     4201 aagcgttgcc gcggatgctt tcctccacgt ccatgctcgc gaggttgcgg tacagcatga
     4261 cggtgggacg tagcaggaac tcgcccagcg acatcacggg aaattccagc ggaaatccgc
     4321 ctgcctcgta catgccgatc ttgacctgct cggctagcgt gcggaagtgg ctgttacagg
     4381 ggtgagctcg atgaaggagt tgcagattcc gatcacgggg cgcccgtcga acgggtcatc
     4441 cgaaacacct ttgcccttga cccagctgcg gtaagcaaaa ccatcccggt cctggcgacc
     4501 aaaccattgc tggctgtgct gttcctcgag ttttttattg ggaggagagg gcggggtttt
     4561 tagggtagta atagggaaag tccttaattt ataaacaaat tagggtcata ccattgccat
     4621 cgtggaatca gtaaatacca taatcgcaga aacttccttg tcccgtccgc atatccttac
     4681 cgtcgccaag ctgccaccgt ttttgatgga gccgctgaat gctgcttttg ttgtgcacga
     4741 ccggctccac gagagtgacc cggcggcatt tacgaaagtc gcgcccgaaa tccgtggcat
     4801 tgccgggggc ggcgagtcca aggtgccgcg ctccttgatg gaccagctgc ctgcgctcga
     4861 aattgtgtcc atcatgggcg tgggctacga cggagttgac gtccccgccg cgctggagcg
     4921 ccatgtgccc gtgacccata ccccgggcgt actgactgat gaagtggccg atctggccat
     4981 aggtctcatg ctatctgtgg cacgtcgcat cccgcaagcc gaccagtacg tgcgcacggg
     5041 ccgctgggcc agggatggcg ccatgccgct gacgcgcaag gtttcgggcg cgcgccttgg
     5101 gatcgtcggg ctggggcgca tcgggcaggc gattgcgagc cgggccgaag catttggcat
     5161 gtcgatcgct tacacggcac gcagcgccaa gccgggacta tcctacaatt tttaccccac
     5221 ggcgcaggcc ctggcggcgc aggttgattt ccttgtggtc atcacg
//
LOCUS       DASUIV010000007         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_652055, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000007 DASUIV010000000
VERSION     DASUIV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652055"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFW59_00215"
     CDS             complement(<1..284)
                     /locus_tag="VFW59_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294555.1"
                     /note="catalyzes the decarboxylation of
                     3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate decarboxylase"
                     /protein_id="HEX5362670.1"
                     /translation="MKYKDLRDFIAQLEKLGELKRVTAPVSTHLEMTEICDRVLRAQG
                     PAILFENVAGHSMPVLANLFGTPRRVALGMGEEDITALREVGKLLAYLKEP"
     gene            complement(387..1256)
                     /locus_tag="VFW59_00220"
     CDS             complement(387..1256)
                     /locus_tag="VFW59_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEX5362671.1"
                     /translation="MLARLGIFILWLIHFLPFRAIVAIGNGVGLMLHLLAADRRRIGD
                     INLRLCFPDLDNAARAKLLRDHFKMFGRGLIERCILWWASPERICSLIRVEGIEHFEA
                     IKNQPAILLTPHFVGMDAGGQWVAQHTNTVCMYANQKSAYLTKLLKDKRARFRNQHLY
                     ARQEGLRPIIKGMRAGMPFIYPPDQDQGVKDGAFIPFFGIPAATMTSVPRIAQMTGAA
                     VVPSITRILPDGKGYVLTFYPAWNNYPSGDAVADGRRMNAFIEDRVREMPEQYFWLHK
                     RFKTRPEGEASFY"
     gene            complement(1256..2134)
                     /locus_tag="VFW59_00225"
     CDS             complement(1256..2134)
                     /locus_tag="VFW59_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5362672.1"
                     /translation="MKFSLMGGLFDLLARLPLSALHRLGAILGWATYALSGRYAARLR
                     ENLKNAGLSHSDTEFRELLHANIREAGKGVMELPWIWRRPFEEVIASVAECHGWEHLE
                     QAHAQGKGIIVLTPHLGCFEIISLYVSARYPVTSMYRPPRQKSLDSLMHEGRARGQMK
                     LVAADMSGVRQLLKALRRGEIIGILPDQAPGSGEGEWAPFFGRPAYTMTLIGRLMEVS
                     KAPVVICHGERLPQGKGYALHFSPLNLDPAGSIPKQMNAALENVIRSCPAQYLWSYNR
                     YKVPRGALPPDTSKEL"
     gene            2210..3373
                     /gene="metK"
                     /locus_tag="VFW59_00230"
     CDS             2210..3373
                     /gene="metK"
                     /locus_tag="VFW59_00230"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5362673.1"
                     /translation="MSEYFFTSESVSEGHPDKVADQISDAILDAILAQDPYARVAAET
                     LTNTGLVVLAGEITTTANVDYIHVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSPD
                     IAQGVDRASDDYLNQGAGDQGLMFGYACTETDTLMPLPIYLSHRVVERQAQLRHDGRL
                     PWLRPDAKSQVTIKYVDGKPFCIDTVVLSTQHAPEMTLEQIREAAIEEIIKPVLPKEL
                     IKGDIKFLVNPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVAAGLAEKCLIQISYAIGVAQPTSVMVTTYGTGKVSDEKISELVKK
                     HFDLRPKGIVQMLDLLRPIYQNTAAYGHFGREEPGFTWENTDKAAALRADAGL"
     gene            3602..4831
                     /locus_tag="VFW59_00235"
     CDS             3602..4831
                     /locus_tag="VFW59_00235"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5362674.1"
                     /translation="MLKRIAIEQLRLGMHIHEFCGSWMDHPFWRGAFLLDSQDDLKAI
                     CATGLKEVWIDTSKGLDIQGGVTEDEVVAEVEATLAHVEMSGNAVQRVDVAQEAVRAA
                     KICARSKEAVSSMFQEARMGKALNADDALPIVEEISNSVMRNPGALISLARLKSKDEY
                     TYMHSVAVCALMVALARTLKLDADQVRQAGLAGLLHDVGKMKIPAAILDKPGKLTEAE
                     FETIKSHPAQGHKMLLAGVGVSEAALDVCLHHHEKVDGSGYPKGLAGEQISLYAKMGA
                     VCDVYDAITSNRPYKNGWEPAESIRKMAEWSKGHFDSWVFEAFVRSIGIYPVGSLVRL
                     ESDRLAVIVEQTDSSLLRPILMVFFSIKANARIQPELLDMSKPACKDKIVAHESPAKW
                     NIDNLQELWSGIAGTPW"
     regulatory      4855..4929
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            4955..>5773
                     /locus_tag="VFW59_00240"
     CDS             4955..>5773
                     /locus_tag="VFW59_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5362675.1"
                     /translation="MNAAFTDYKVADMSLAPWGRKEIAIAETEMPGLMAIRAEFAKAQ
                     PLKGARITGSLHMTIQTAVLIETLTALGAEVRWASCNIYSTQDHAAAAIAAENIPVFA
                     FKGESLTDYWDFTHRIFEWTDGGLSNMILDDGGDATLLLHLGARAEKDATLLNNPGSE
                     EETCLFASIKAKLAADKTWYSTRLAAIKGVTEETTTGVHRLYQMHERGELKFPAINVN
                     DSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVICGYGDVGKGSAQAMRALS"
BASE COUNT         1130 a         1650 c         1857 g         1136 t
ORIGIN      
        1 ggttccttga gataggcgag cagtttgccg acttcgcgca gtgcggtgat gtcttcctcg
       61 cccatgccca gtgcgacgcg gcgcggtgta ccgaacaggt tagcgagcac tggcatgctg
      121 tgtccggcga cgttctcgaa caggatggcc gggccctgtg cgcgcagcac gcggtcgcag
      181 atctcggtca tctccagatg cgtggagacg ggggcggtca cgcgcttgag ttcgccgagc
      241 ttttccagtt gtgcgatgaa gtcgcgcagg tctttgtatt tcatggggcc ctctttttca
      301 ggccctcttc ctctgcccct ctcccgctag cgggagaggg gagtatctcc ctcgccccat
      361 aggggagagg gttggggaga gggggttcaa taaaaacttg cttccccttc ggggcgcgtc
      421 ttgaaacgtt tgtgcagcca gaaatactgc tccggcattt cgcgcacgcg gtcttcgatg
      481 aatgcgttca tccggcggcc gtcggcgacg gcgtcgccgg agggatagtt gttccaggcg
      541 ggatagaagg tcagcacata acctttgccg tccggcaaga tgcgggtgat gctgggcacg
      601 accgcagcac cggtcatctg cgcgatgcgc ggcaccgagg tcatggtcgc ggcgggtatg
      661 ccgaagaagg ggatgaacgc gccgtctttc acgccctgat cctgatccgg cggatagatg
      721 aagggcatgc ctgcgcgcat ccccttgatg atcgggcgca gtccttcctg tcgcgcatac
      781 aggtgctggt tgcggaaacg cgcgcgcttg tctttgagca gcttggtcag ataggcgctc
      841 ttctggttcg cgtacatgca tacggtgttg gtgtgctgcg cgacccactg gccgcctgca
      901 tccatgccga cgaaatgcgg cgtgagcagg atcgcgggct ggttcttgat ggcctcgaaa
      961 tgctcgatgc cttccactcg gatcaggctg cagatgcgct caggactggc ccaccacagg
     1021 atgcagcgtt cgatcaggcc gcgcccgaac atcttgaagt ggtcgcgcag cagctttgcg
     1081 cgcgccgcgt tgtccagatc gggaaagcag agcctgaggt tgatgtcgcc gatcctgcgg
     1141 cggtcggcgg ccagcagatg gagcatcagc cccacgccgt tgccgatggc gacgatggcg
     1201 cggaacggca ggaaatgaat cagccacagg atgaagatgc cgaggcgtgc cagcattaaa
     1261 gttccttcga ggtgtccggc ggcaatgcgc cgcgcgggac tttgtaacgg ttgtagctcc
     1321 acaggtattg cgcggggcag gagcggatca cgttttccag cgcggcattc atctgcttcg
     1381 ggatggagcc tgcggggtcg aggttcagcg gcgagaaatg cagcgcatag cctttgcctt
     1441 gcggcaggcg ttcgccgtgg cagatcacca ccggtgcctt gctgacttcc atcaggcgtc
     1501 cgatcaaggt catggtgtag gcggggcggc cgaagaacgg cgcccattcg ccctcgccgc
     1561 tgccgggggc ctggtcgggc aggatgccga tgatttcgcc gcgccgcagc gccttgagca
     1621 gctggcgcac gccgctcatg tcggccgcga ccagtttcat ctggccgcgc gcgcgtccct
     1681 cgtgcatcag cgagtcgagc gatttctggc gcggcggacg gtacatgctg gtcaccggat
     1741 agcgcgcgga gacatacagg ctgatgatct cgaagcagcc cagatgcggg gtcagcacga
     1801 tgatgccttt gccctgtgca tgcgcctgtt cgagatgttc ccagccgtgg cactcggcga
     1861 cgctggctat cacctcttcg aacgggcgcc gccagatcca gggcaattcc atcacgcctt
     1921 tgcccgcctc gcggatattg gcgtgcagca gttcgcggaa ctcggtatcg gaatggctca
     1981 gcccggcgtt cttcaggttc tcccgcaggc gtgcggcata gcgcccggag agcgcatagg
     2041 tcgcccagcc gagtatcgcc ccaagccggt gcaacgccga caagggcagg cgagccagca
     2101 ggtcgaataa tccgcccatc agggaaaatt tcataaagtc taattgaacg agggttgttg
     2161 ctataatgcg caccctattt aaccactgta gtgatcgaaa cggagagcca tgagcgaata
     2221 tttctttaca tccgaatccg tgtccgaggg ccatccggac aaggttgccg accagatttc
     2281 cgacgcgatt ctggacgcga ttctagcgca agacccctat gcgcgggtag cggccgagac
     2341 gctgaccaac accggactgg tggtgctggc cggtgagatc actacgacag ccaacgtcga
     2401 ttacatccac gttgcgcgcg acaccatcaa acgcatcggt tacgacaaca ccgaatacgg
     2461 catcgactac aagggctgcg ccgtgctggt ggcctacgac aagcagagcc cggacatcgc
     2521 ccagggcgtg gaccgcgcct ccgacgacta cctgaatcag ggtgcgggcg accagggctt
     2581 gatgttcggt tacgcttgca ccgaaaccga caccctgatg ccgttgccga tctatctgtc
     2641 gcaccgcgtg gtcgagcgtc aggcgcaatt gcgtcacgac gggcgtctgc catggctgcg
     2701 tccggatgcg aagtcccagg tcaccatcaa gtatgtggac ggcaagccgt tctgcatcga
     2761 caccgtggtg ctgtcgaccc agcatgcgcc tgaaatgacg ctggagcaga tccgcgaagc
     2821 cgcgatcgaa gagatcatca agccggtgct gccgaaggaa ctgatcaagg gcgacatcaa
     2881 gtttttggtg aatccgaccg gccgtttcgt catcggcggc ccgcagggtg actgcggcct
     2941 gaccggacgc aagatcatcg tcgacaccta cggcggcgcg gctccgcacg gcggcggcgc
     3001 gttctccggc aaggatcctt ccaaggtcga ccgttcggca gcctatgccg gccgctatgt
     3061 ggcgaagaac atcgtcgccg ctggtctggc cgagaagtgc ctgattcaga tctcctatgc
     3121 gatcggcgtg gctcagccga ccagtgtgat ggtgacgacc tacggtaccg gcaaggtttc
     3181 cgacgagaag atctcggaac tggtgaagaa gcacttcgac ctgcgcccga aaggcatcgt
     3241 gcagatgctc gacctgctgc gtccgatcta ccagaacacc gccgcctacg gccacttcgg
     3301 tcgcgaagag ccagggttta cgtgggagaa cactgacaaa gcagccgcgc ttcgcgcgga
     3361 tgccgggctg taagcccgcc gcgaagcggt ttgtcattgt tcgggtgaag ggagcgtaag
     3421 ctcccgtgcc gaagccaaaa cactgacaag aaggtgtagc tcttcttgcg gctccgggcg
     3481 tagcccgacc tcgcaatgta gtaattggca acaaatcaaa gccgcccccg aggaaactcg
     3541 ggggcggttt ttttcatata gaatccagac gtcccctgac gagtcacggt acgaagcgct
     3601 tatgctgaaa cgtattgcca ttgagcagtt gcgattgggt atgcatattc acgagttttg
     3661 cggctcgtgg atggatcatc cgttctggcg tggcgccttt ctgctggaca gtcaggatga
     3721 tttgaaggct atctgcgcca cgggtctcaa ggaggtgtgg atcgacacct ccaaggggct
     3781 ggatatccag ggcggtgtga ccgaggatga ggtggtggcg gaggtggagg ccacgcttgc
     3841 gcatgtcgaa atgtctggca atgccgttca gcgtgtcgat gtcgcgcagg aggcggtgcg
     3901 tgcggcgaag atctgtgcaa gatccaagga ggcggtctcc tcgatgtttc aggaggcgcg
     3961 catgggcaag gccttgaatg cggatgatgc gttgcccatc gtcgaggaga tatccaactc
     4021 ggtgatgcgc aatccgggcg cgctgatcag tctggcgcgt ttgaagagca aggacgaata
     4081 cacctatatg cattcggttg cggtgtgcgc gctgatggtt gcgttggcca gaaccttgaa
     4141 gctggatgcc gatcaggtga ggcaggccgg tctggcgggt ttgttgcacg atgtcggcaa
     4201 gatgaagatt ccggccgcga tcctcgacaa gccgggcaag ctgaccgagg ctgaattcga
     4261 aacgatcaag agtcatcccg cccaagggca caagatgctg ctggcggggg tgggcgtcag
     4321 cgaagccgcg ctagacgtct gtctgcatca tcacgagaaa gtggatggca gtggttatcc
     4381 gaaagggttg gcaggcgagc agatcagtct ctatgccaag atgggggcgg tgtgcgatgt
     4441 gtatgacgcg atcacctcca atcggcccta taaaaatggc tgggaacccg ccgaatccat
     4501 ccgcaagatg gccgagtgga gcaaggggca tttcgattcg tgggtattcg aggccttcgt
     4561 gcgcagcatc ggcatctatc cggtcggctc cctggtcagg ctggagtcgg atcgtctggc
     4621 tgtgatcgtc gagcagaccg atagctcctt gctcaggccg atattgatgg tgtttttctc
     4681 gatcaaggct aacgcgagaa ttcagccgga gttgctggat atgtcgaaac cggcctgcaa
     4741 ggacaagatc gttgcgcatg aaagtccggc caagtggaat atcgataatc tgcaggaatt
     4801 atggagcggc atcgccggta caccctggta gggcaggttg cgctataatc cgccccctcc
     4861 gaggggtgct gcagcgggtt gatcgtcttt ccccgccagg ctcggaagtc caaacggcac
     4921 ccattcatta acccttatat gaatggagtt tgttatgaat gcagctttca ccgactataa
     4981 agtcgccgac atgtccctgg ccccatgggg ccgcaaagag atcgcgatcg ccgagaccga
     5041 gatgcctggt ctgatggcca tccgtgcaga attcgccaag gcccaaccgc tgaaaggcgc
     5101 gcgcatcacg ggctcattgc acatgaccat ccagaccgcc gtgctgatcg agacgctgac
     5161 cgcgttgggc gcagaagtgc gctgggcatc gtgcaacatc tactccactc aggatcacgc
     5221 tgccgctgcg atcgccgccg agaatattcc ggtgttcgcc ttcaagggcg agtcgctgac
     5281 cgattactgg gacttcactc accgtatctt cgagtggacc gatggcggtc tgtcgaacat
     5341 gattctggac gatggcggcg acgcgacgct gctgctgcat ctgggcgcgc gcgccgagaa
     5401 ggatgcaacg ctgctgaaca atcccggctc cgaagaggag acctgtctgt tcgcgtcgat
     5461 caaggccaag ctggcggccg acaagacttg gtactccacc cgtctggccg cgatcaaggg
     5521 cgtgaccgaa gagaccacca ccggcgtgca ccgcctgtac cagatgcacg agcgcggcga
     5581 actgaagttc ccggcgatta acgtcaacga ctccgtgacc aagtccaagt tcgacaacct
     5641 gtatggctgc cgcgagtcgc tggtggacgg catcaagcgc gccaccgacg tgatgatcgc
     5701 gggcaagatc gcggtgatct gcggttacgg cgatgtgggt aagggttccg cacaagccat
     5761 gcgcgcattg tcc
//
LOCUS       DASUIV010000008         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_280598, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000008 DASUIV010000000
VERSION     DASUIV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280598"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFW59_00245"
     CDS             <1..942
                     /locus_tag="VFW59_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5362676.1"
                     /translation="VSHVCGHDLHVTWLMGAARLLAEHRAAWHGTLLAVFQPGEEVGR
                     GAQGMMDDGMAERFPKPEVIFGQHVMVGEAGTVGYRSGTILSAGDSLKVKLFGRGGHG
                     SMPHNSVDPVVMAASTVMRLQTIVAREVAPLDSAVVTVGALQAGTKENIIPDDATLKL
                     NIRTYSEGVREHVLAAVKRICCAECTASNAPREPEFTTLNAYPLTENDAAATARVAAA
                     FRSQFAERAFETPPAPASEDFSIFGRRWGVPYVFWFVGGTDAQTYREAKARGQLNAIP
                     SNHSPQYAPVLHPTLKTGLQAMLTAASAWLCAGAAEV"
     gene            939..1574
                     /locus_tag="VFW59_00250"
     CDS             939..1574
                     /locus_tag="VFW59_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX5362677.1"
                     /translation="MNAQTLFAALDLAGVFACAISGATAALQRRLDLFGVVMVAFITA
                     VGGGVVRDLCVGATPPVGLSDWTYLAVAVLAALLTIGAGSLVERLAYPVLLFDALGLS
                     VFAVAGAHKALAYGHNYEVAILLGTTTAVGGGLARDVLLNRVPAILRKEIYATAALLG
                     ASILVLGEALGWSRNWLTWLAIAACFALRLLSLRYGWHLRLSVAGPRSGEP"
     gene            complement(1809..3110)
                     /locus_tag="VFW59_00255"
     CDS             complement(1809..3110)
                     /locus_tag="VFW59_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362678.1"
                     /translation="MSEFSVELAAEQIVDARTKSYFTEVLSSFINGHYRSTAVMLWSV
                     VISDLVYKLQELRDLYQDPVATSILDSVGARQAANPTNPEWEPYLLDEVNSRTQFFEA
                     GEHQHIVAIHKLRHLSAHPVLSSAHLLFEPNKETARAAIRNALECVLLKPPVFSRKVV
                     INLIEDLAARRALLPDTTSLRMYLEAKYLRSLHPTVKLELIRTLWKFCFRLSNPDADA
                     NREINFRALKVIHDRDALDFRNVIRQNADQFSEVAAGGAPLMAVVRFLAKCDGIYPTL
                     THAAKIPIAAFARTDVNLLADASYINDSLAAHIAELSGLPVEQLSNLSDDSWLALLAK
                     ADGANLRQEALEIAIKLYCASSGFNTADSLFARFIRLHAAEFDTTRMTQVLAGIERNN
                     QTYWRWQARTDHPLLKARADELGVDLAPYPNFRESLPQQVG"
     gene            complement(3375..3806)
                     /locus_tag="VFW59_00260"
     CDS             complement(3375..3806)
                     /locus_tag="VFW59_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362679.1"
                     /translation="MMMFMIGGFNPEMPWHAHKVAELYSKKLAKLGSLLSIGSGALFF
                     VPLTQGMAFALASLLLISAIYFGSEYRKHVDEVVKQTPMLKLIKIFAPTWRYFAMTTI
                     LLGVGLGLIEWSFFGEQKHLLISLIVSGGILDVIKGYFASK"
BASE COUNT          743 a         1213 c         1253 g          801 t
ORIGIN      
        1 gtttcccatg tctgcggcca cgacctgcac gtgacctggc tgatgggcgc ggcgcgcctg
       61 ctggccgagc accgcgctgc ctggcacggc accctgctgg cggtgttcca gccgggcgag
      121 gaggtcgggc gcggcgcgca gggcatgatg gacgacggca tggccgagcg ctttcccaag
      181 cccgaggtga tcttcggcca gcacgtgatg gtcggcgaag ccggcaccgt cggctaccgc
      241 agcggcacca tcctctccgc cggcgacagc ctcaaggtca agctgttcgg ccgcggcggc
      301 cacggctcga tgccgcacaa ctcggtcgac ccggtggtga tggcggcgtc caccgtgatg
      361 cgcttgcaga ccatagtcgc gcgcgaagtc gcgccgctgg acagcgcggt ggtcaccgtc
      421 ggcgcgttgc aggccggcac caaggaaaac atcatccccg acgacgccac cctcaagctg
      481 aacatccgca cctacagcga aggcgtgcgc gagcatgtgc tggccgcggt gaagcggatc
      541 tgctgcgccg agtgcacggc gtccaacgcg ccgcgcgagc cggagttcac cacgctcaac
      601 gcctatccgc tgaccgagaa cgacgccgcc gcgaccgcgc gggtggctgc cgccttccgc
      661 agccagttcg ccgagcgcgc cttcgaaacc ccgccggccc cggccagcga ggacttcagc
      721 atcttcggcc ggcgctgggg cgtgccctac gtgttctggt tcgtcggcgg caccgacgcg
      781 cagacctacc gggaggccaa ggcgcgcggg cagctcaacg ccatccccag caaccactcg
      841 ccgcagtacg cgccagtgct gcatcccacc ctgaagaccg gcctgcaggc catgctgacc
      901 gccgcctcgg cctggctgtg cgcgggcgcc gcggaggtat gaacgcacag accctgttcg
      961 ccgcgctgga tctcgccggg gttttcgcct gcgccatcag tggcgccacc gccgccctgc
     1021 agcggcgcct cgacctgttc ggcgtggtga tggtcgcctt catcaccgcg gtcggtggcg
     1081 gtgtggtgcg cgacctgtgc gtcggcgcca ctccgccggt cggcctgagc gactggacct
     1141 atctggccgt cgcggtgctt gccgcgctgc tcacgatagg ggccggctcg ctggtcgagc
     1201 gcctggccta cccggtgctg ctgttcgacg ccctcggcct cagcgtgttc gccgtcgccg
     1261 gcgcgcacaa ggcgctggcc tacggccaca actacgaggt ggcgattctc ctcggcacca
     1321 ccaccgcagt cggcggcggc ctcgcccgcg acgtgctgct caaccgcgtg ccggcgatcc
     1381 tgcgcaagga aatctacgcc accgccgcgc tgctcggcgc cagcatcctg gtgctcggcg
     1441 aagcgctggg ctggtcgcgt aactggctga cctggctggc gatcgcggct tgcttcgcct
     1501 tgcgcctgct gtcgctgcgc tatgggtggc atttgcggct gagcgtggcc ggaccgcgct
     1561 ccggcgaacc ctagacttcg atggctctca cgctcctgcg tgggagcctg aacgatggac
     1621 gctccgcgtc cagccgcagg cgcggcgcgt gtgcccacgc gggagcgtgg gcacgatcat
     1681 caagacaagc cccgggaagt cggggacaaa ccacggtttc gccagccgta ggggggcaac
     1741 tgccgcaggc attgcccacc ttgggacgtc gcgccggtgc tgtggtagaa aatcgctgcg
     1801 cgagttttct accctacttg ctgaggcagt gattctctga agttcgggta gggtgctaga
     1861 tcaactccaa gctcatcagc acgcgctttg agaagaggat gatcggtccg cgcctgccag
     1921 cgccaatagg tctgattgtt gcgctcgatg cctgctaaca cctgggtcat cctcgtggtg
     1981 tcaaactccg cagcatgaag tcgaataaac cttgcgaaga gcgagtcagc ggtgttgaag
     2041 cctgaacttg cgcagtagag tttgattgcg atctcgagtg cttcctgcct gagatttgcc
     2101 ccatctgctt tggcaagaag cgcgagccaa ctgtcatcgc ttaggttgga tagctgctca
     2161 actggcaggc cggatagctc cgcgatgtgc gcggccaagc tgtcattgat gtacgaggca
     2221 tcggcaagca gattgacatc ggttcgtgcg aaggctgcga tgggaatctt tgcggcatgc
     2281 gtgagcgttg ggtaaatgcc atcacatttc gctaggaatc gaacaacggc catcagcggt
     2341 gcgcctccag cggctacttc actaaactgg tcggcgttct ggcgtatgac attccgaaag
     2401 tccagtgcat cgcgatcatg aatgactttc agtgctctaa aattgatctc tctgtttgcg
     2461 tctgcgtcgg ggtttgagag tcgaaagcag aacttccaga gcgtacgaat gagttccaat
     2521 tttaccgttg gatgaagact cctgaggtac ttcgcctcta agtacattcg taacgaggtg
     2581 gtgtctggaa gtagagctct gcgcgcagcc agatcctcta tgaggttgat tacgaccttt
     2641 cttgagaata cgggaggctt tagtaaaacg cactccaagg cgttgcgaat ggccgcgcgc
     2701 gctgtttctt tgttgggttc gaagagaagg tgcgcgctac taagaaccgg atgagccgac
     2761 aggtgacgaa gcttatggat agcgacgatg tgttgatgct cgcccgcttc gaagaactgc
     2821 gtgcggctgt tgacttcgtc aagcaaatag ggctcccact cgggattggt tgggttggcg
     2881 gcttgccgag ccccgacaga atctaggatt gacgtcgcta ccgggtcttg gtacaggtca
     2941 cgcagttctt gaagcttgta gacgaggtcg gatatgacca ctgaccagag catgacggct
     3001 gtggaacggt agtgaccatt gatgaacgaa gacagcactt cagtgaaata cgacttggta
     3061 cgggcatcaa cgatctgctc agcagctaac tcgacggaga actcgctcat ggggtgtgaa
     3121 ctcgggggga tatgaggcct aacatctaag gtaagtggcg cgccgcttgc ggcgcgtccc
     3181 gcttgactgc tggggttttg atggctccca cgctccagcg tgggagcccg aacactggac
     3241 gcttcgcgtc cagccgcagg agcggccaat acgcgggagc gtgggcatga tcatcatacg
     3301 taactatttt ggggtttgag tgttgtttgg caaattagat ggggctttgc cacggccccg
     3361 taccccgtgt tcactcactt tgaggcgaaa tatcccttga taacatcgag gatcccgcct
     3421 gagacaatga ggctaatgag tagatgcttc tgctcaccga agaaactcca ctcgatcagg
     3481 ccgagcccaa cgcccaagag gatcgttgtc attgcgaagt accgccaggt aggcgcaaag
     3541 atcttgatga gcttgagcat aggggtttgc ttgactacct catcgacatg tttacggtat
     3601 tcactaccaa agtagatcgc cgagatcagc agtagggatg cgagcgcaaa ggccatgccc
     3661 tgagtcagcg ggacgaagaa cagcgcaccg gatccgatgc tcagcaggct tcccaacttg
     3721 gccaattttt tgctgtatag ctcggcaacc ttgtgggcat gccaaggcat ttcgggattg
     3781 aaaccaccaa tcatgaacat catcatctcc ttagtgcacg ccttcagcta gaggcgaggc
     3841 atgttcttct ttgctttacg aattcccgca gaaggggaat gcgtgattag tcgccgagcg
     3901 gggattctcc ctcactcctt cccataattc accaccgaca acagcttgat cggcactttc
     3961 actagctgct ccgggccgtg cggcacttcg ccgtcgaagg tcaggctgtc
//
LOCUS       DASUIV010000009         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1303082, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000009 DASUIV010000000
VERSION     DASUIV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303082"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /gene="purL"
                     /locus_tag="VFW59_00265"
                     /gene_synonym="purI"
     CDS             complement(<1..1802)
                     /gene="purL"
                     /locus_tag="VFW59_00265"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX5362680.1"
                     /translation="MFQVSVGTSALSSFRLEKLRAVLSDVAPGVVVADTRHCYFSALK
                     SSTLSKAQAQLLNKVLGIDKSAGEPGDAASCQRLLVLPRLGTISPWSTKASDIAQHCG
                     LSSVTRIERGVVYYLSTKGGKALSKDELAAVLPLLHDRMTETVLTAIEGAAEQIFQHG
                     KPQPLATVDVLKGGAKALDKANREMGLALSADEIEYLVENFRKLKRNPTDVELMMFAQ
                     ANSEHCRHKIFNADWVIDGEQQDISLFGMIRNTHKVSPEGTVVAYSDNSSVIEGARIE
                     RFYPRADGSYAFSDEMTHILMKVETHNHPTAIAPFAGAATGSGGEIRDEGATGSGSKP
                     KAGLSGFSVSNLNIPGFGQPWEAPYGKPSRIVTALQIMLDGPIGSAAFNNEFGRPNLT
                     GYFRTFEENVSGEMRGYHKPIMLAGGVGNIAANHTHKHDLPEGALVIHLGGPGMLIGL
                     GGGAASSMDTGSNAENLDFDSVQRGNPEMQRRAQEVIDRCWQLGENNPILSIHDVGAG
                     GMSNALPELVHGGGKGARFDLRKIPLDETGMSPKQIWCNESQERYVLAIAPERLDEFR
                     AMCERERCPFAVVGTAIDEDQIVVHDKEFKNDPV"
     gene            1873..2769
                     /locus_tag="VFW59_00270"
     CDS             1873..2769
                     /locus_tag="VFW59_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX5362681.1"
                     /translation="MSKRIPALTRKQVIANFPQRQHDSHKGQFGTVAVIGGASGMLGA
                     PLMAARAALKLGAGCVHVGLLADNAPGVDLLQPELMLHKAADLLGSSPLPNPLPQAGE
                     GANAKSNFDVLAIGCGLGRSLAAQKLLHDAISSSAALVLDADALNLLAQRPDLRSALL
                     ARKHATVLTPHPGEAARLLGCEIAEVQADRTAAVQALAQKFGGSIVLKGADSLCATSD
                     GKLYVNQTGNPGMSAAGMGDVLTGMIAALIAQGMSADDALLLAVHLHGAAGDELAKQQ
                     ATIGMSATEVTEWARWLLNQWI"
BASE COUNT          539 a          954 c          903 g          547 t
ORIGIN      
        1 tcgaccgggt cattcttgaa ttccttgtcg tgcaccacga tctggtcttc gtcgatcgcg
       61 gttcccacca ccgcgaacgg gcagcgctcg cgctcgcaca tcgcgcggaa ctcgtcgaga
      121 cgctccggcg cgatcgccag cacgtaacgc tcctgagact cgttgcacca gatctgcttc
      181 ggcgacatgc cggtctcgtc cagcgggatc ttgcgcaggt cgaaacgcgc gcccttgccg
      241 ccgccgtgca ccagctcggg cagcgcgttg gacataccgc ccgcgccgac gtcgtggatg
      301 gacagaatgg gattgttctc ccccagctgc cagcagcggt cgatcacctc ctgtgcgcgg
      361 cgctgcatct cgggattgcc gcgctgcacc gaatcgaagt ccagattctc ggcattgctg
      421 ccggtatcca tgctcgatgc cgcgccgccg cccagaccga tcagcatgcc ggggccgccc
      481 agatggatca ccaaggcgcc ttccggcagg tcgtgcttgt gggtatggtt agccgcgatg
      541 ttgccgacgc cgcccgccag catgatgggc ttgtggtaac cgcgcatctc gccgctgacg
      601 ttctcttcga aggtgcggaa atagccggtc aggttcgggc ggccgaattc gttgttgaac
      661 gccgcgctac cgatcgggcc gtccagcatg atctgcagcg cagtgacgat gcgcgacggc
      721 ttgccgtaag gcgcttccca gggctgtccg aagccgggga tgttcaggtt cgacacggag
      781 aaaccgctca ggccggcctt gggctttgag ccgctgccgg tcgcgccctc gtcgcgtatc
      841 tcgccgccgg aaccggtcgc ggctcccgcg aatggcgcga tggcggtcgg gtggttgtgc
      901 gtctccacct tcatcaggat gtgggtcatc tcgtcgctga acgcgtagct gccgtcggca
      961 cggggataga agcgctcgat gcgcgcgccc tcgatcaccg acgagttgtc ggaataggcc
     1021 accaccgtgc cctcagggct gaccttgtgg gtgttgcgta tcatgccgaa caaggagatg
     1081 tcctgctgct cgccgtcgat cacccagtcg gcgttgaaaa tcttgtggcg gcaatgctcg
     1141 gagttcgcct gcgcgaacat catcagctcg acgtcggtcg ggttgcgttt cagcttcctg
     1201 aagttctcga ccagatactc gatctcgtcc gccgacagcg ccagccccat ctcgcggttg
     1261 gccttgtcca gcgccttggc gccgcccttg agcacgtcca ccgttgccag aggttgcggc
     1321 ttgccatgct ggaatatctg ttctgccgcg ccttcgatcg cggtcagcac ggtttcggtc
     1381 atgcggtcgt gcagcagcgg cagcactgcg gccagctcgt ccttgctcag agccttacct
     1441 cccttggtcg acaggtaata caccacgccg cgctcgatgc gcgtcaccga ggacaatccg
     1501 caatgctgcg cgatgtcgga agccttggtg gaccacggcg agatcgtgcc cagacgcggc
     1561 agcaccagca gacgctggca tgaagccgca tcgcctggct cgcccgccga cttgtctatg
     1621 cccagcacct tgttcagcaa ctgggcttgc gccttgctca gcgtcgaact cttgagtgcg
     1681 ctgaaatagc agtggcgcgt gtccgcgacg accacaccgg gagcgacatc gctcaacaca
     1741 gcgcgcaatt tttcaagacg gaatgaggat aaggcagagg taccaacaga aacttgaaac
     1801 atagcaggaa ccatgaaaat gaattcgggg cgcgattata ctatgggcct caccatcaga
     1861 actcgaccag aaatgagcaa acgcatccct gccctcaccc gcaagcaagt catcgccaat
     1921 tttccgcaac gccagcacga cagccacaag ggccagttcg gcacggtcgc cgtgatcggc
     1981 ggcgcatccg gcatgctcgg cgccccgctg atggcggccc gcgcggcgct caaactgggc
     2041 gcaggctgcg tgcatgtcgg cctgctggcg gacaacgcgc cgggagtgga tctgctgcaa
     2101 ccggagctga tgctgcacaa ggctgcggat ttgcttggtt ccagccctct ccccaaccct
     2161 ctcccgcaag cgggcgaggg agcaaacgcg aaaagcaact ttgatgttct tgcgatcggc
     2221 tgcgggctgg gacgcagtct tgctgcgcag aagctgctgc atgacgcgat cagcagctcc
     2281 gccgcgctgg tgctggatgc cgacgcgctg aacctgctcg cgcagcgccc cgacctgcgc
     2341 agtgcgttgc tcgcgcgcaa gcatgccacc gtgctcaccc cgcatcccgg cgaagccgcg
     2401 cgcctgctgg gctgcgagat cgccgaagta caggcagacc gcacagcggc agtgcaggca
     2461 ctggcacaga agttcggcgg cagcatcgta ctgaagggtg cggacagcct gtgcgcaacg
     2521 agcgacggca agctctacgt caaccagacc ggcaatcccg gcatgagcgc tgccggcatg
     2581 ggcgatgtac tgaccggcat gatcgcggcc ctcatcgccc agggcatgag tgccgacgac
     2641 gcgctcctgc tggcggtgca cctgcacggt gcggcgggcg acgaactggc gaaacagcaa
     2701 gccaccatcg gcatgagcgc gaccgaggtg accgagtggg caaggtggtt gttgaatcag
     2761 tggatttaag ctgaatttaa aatcaccttg gcacacctct gcacaggcag gccgatgtcc
     2821 ttcatgtcgt cggtctactg agaaataagg gataaattac tttcttctcg aagcaatgcc
     2881 atcgaaattc cccgtcaaac ttgcaatcgt tcgccatggc atctgaaagc cccttgcaac
     2941 tca
//
LOCUS       DASUIV010000010         8845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_931574, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000010 DASUIV010000000
VERSION     DASUIV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931574"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFW59_00275"
     CDS             <1..892
                     /locus_tag="VFW59_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HEX5362682.1"
                     /translation="MDALITFAPFLLAVMIAAAFSPLLLNGWLFSIGALQPKFSKLNP
                     IAGIGRMFSQTSLVELVKALAKAGLIGGVSAWVIWNNRDAVMALGMQSAAASIPAAGH
                     VIGSAFMVIVGAMLLIVAIDVPFQLWDHNKKLMMTKEEVRQESKESEGDPMVKGRIRQ
                     LQREAARNRMMSEIPTADVVVTNPTHYAVALKYSETGARAPVIVAKGSQLLALRIREL
                     AAEHHVPILEAPPLARALHKHTELGETIPEALYTAVAEVLAYVYQLRRYETAGGERPV
                     EPADLPVPVELDPAAGIVAA"
     gene            904..3000
                     /gene="flhA"
                     /locus_tag="VFW59_00280"
     CDS             904..3000
                     /gene="flhA"
                     /locus_tag="VFW59_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX5362683.1"
                     /translation="MNFQLIQDFLKRAGLVGMVGPVLIVLILAMMVLPLPPLLLDMLF
                     TFNIAISIMVLLVSMNTNKPLDFAIFPTVLLITTLLRLSLNVASTRIVLLEGHTGPDA
                     AGKVIESFGHFLVGGNYAVGLVVFAILVVINFVVITKGAGRIAEVGARFTLDAMPGKQ
                     MAIDADLNAGLIGEDVARKRREEIAQEADFYGAMDGASKFVRGDAVAGIIILFINIIG
                     GLIVGVLQHDLDIATAAKNYTLLTIGDGLVAQIPALVISTAAGLVVSRVASEENIGQQ
                     LIKQLFMQPQVLILTAVIIGALGMIPGMPHFAFLLLGGGMAWLAYYLINSGAKAIVLE
                     AEEAAATAATMPVVSESTEASWDDVAQVDVLGMEVGYRLIALVDKTQDGDLLRRIKGI
                     RKKFTQDIGFLPPPVHIRDNLDLRPNSYRITLKGVEVGTGEAFPNMFLAINPGNASGE
                     LAGNPTVDPAFGLPAVWIEGGLRDQAQLMGYTVVDPGTVVATHLSQLLSTRAAELLGR
                     QEVQQLLDHLAKVAPKLVEDLVPKTLPLGVVQKVLQNLLDEGMHIRDMRTIVETLAEG
                     ATRTQDAYQLTSWVRVALGPAIVQQFYPSMQELQVIGMDKELESVLLQAMQSSQNGMG
                     IEPGLADTVLREARAAADQQEQMGLPSVMLVPTPLRDLLARFLKRAVPNLRVISHDEV
                     PDSKTIRVTAMVGGRA"
     gene            2997..4451
                     /gene="flhF"
                     /locus_tag="VFW59_00285"
     CDS             2997..4451
                     /gene="flhF"
                     /locus_tag="VFW59_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEX5362684.1"
                     /translation="MSIRKFFAPTARQALKEIRDELGPDAMILSNRKTESGVEIIALA
                     HGEIANLTGQAVAQKAEWGGGITADEWARSVVDDHAARPVSQARVAPAPSPQLAQQAR
                     NAPQLKQPPQQAQNAPQLKPQQAMPARKAEPVAVSRQPGKLPQPEMQMPLPEPVAVPA
                     NQPVVPGLVAQVQEMLVKEIKSMGSMLQQQISAMHWSDVQQRDPQRTAMLRRMLSAGF
                     STVLARQMLDRMPANGAQGEPWVKQVLKRNLLVAGEANDIIAKGGVYALIGPTGVGKT
                     TTTAKLAARAVVRYGADKVALLTTDSYRIAAYEQLKIYGKILGVAVHAVKDADDLRLT
                     LSALRHKHLVLIDTVGMGQRDQRVSDQADMFEAAGVQCLLMMNATSSGDTLEDVVRMY
                     HNDKVIGCIATKLDEAVSYGAVLDVAVRHRLVMHYMANGQRVPEDLHTINLDYMLHRA
                     FKPLEEKTPFTLCDLDLPVMMSAAESGKEASLAL"
     gene            4441..5385
                     /locus_tag="VFW59_00290"
     CDS             4441..5385
                     /locus_tag="VFW59_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362685.1"
                     /translation="MRSDGVVDQAEGLRRLLVANQTQIVTVVAGKTGMGRTSATINLA
                     AALVRSGKDVLVLDENHAPNNLLDYLGLTARQDLLDVAQGGCLPHEAVLDGHGFGVLP
                     ASRAIHALAISRRSAGSGGGLGRDEWRRMEAALTEVSRGVDVMLVDGAMLAGQAAAVS
                     SSLADGAGLLVVVEATPEGITDSYALIKRLALENSRLQFDIVVNKVASEKAAMTVFDN
                     MSKVARSKLSVRLEYLGYVPLDEKIRYATQMGKSVLDAFPATASAKAYLELAQNLLRQ
                     PMRKDEAESGVAAIIHSLMSQVSQPLRGYSKEIAHVVN"
     gene            5390..6100
                     /locus_tag="VFW59_00295"
     CDS             5390..6100
                     /locus_tag="VFW59_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEX5362686.1"
                     /translation="MYKATGLKDKEHCLREYSHLVKRIAHQMMTRLPHSVQIDDIIQA
                     GMIGLLDAASRYDVFHGAQFETFASQRIRGAMLDELRDADWLPRSLRRDMRRIEQSIS
                     RLQQKLGRAPNEGEIAADLEVPLPEYQQMLQASRGAQLLYYEDFHDEDHDDFFERFEF
                     SSDDNPLELLSNERFRAELVSAIDSLPEREAMVMAMIYEQDMNLREIGEVLGVGESRV
                     SQLHSQAVGRLRSRMKGY"
     gene            6100..6840
                     /locus_tag="VFW59_00300"
     CDS             6100..6840
                     /locus_tag="VFW59_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEX5362687.1"
                     /translation="MDKLTLLGLLVGVTGILTGQVLEGGSLSILLQGAAFVIVFGGTL
                     GAVMVQSPSKVFITAIKMGRWAFKPPQANVDELVHQLSMWGQVARKEGILALEVRVGV
                     VEDPFLKKGLQLLVDGNSGDKIREVLDVDIHSWEQLRWQAARVWEAAAGYSPTIGIIG
                     AVLGLMHVMQNLAEPSKLGAGIAVAFVATIYGVGFANLLFLPIANKLKSILMQQSELR
                     EMIVDGLVGIANGENPRLLEIKLRSYID"
     gene            6882..7643
                     /gene="motD"
                     /locus_tag="VFW59_00305"
     CDS             6882..7643
                     /gene="motD"
                     /locus_tag="VFW59_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HEX5362688.1"
                     /translation="MARRRKRLEHDNHDRWLISYADFVTLLFAFFVVMYSISSVNEEK
                     YKTFGTALNSAFGKQAGTISPSEKSNPQEQSILLMVDPQTARLGAQQRKIQEHMKSLH
                     AGLSQVMADLISQRQVSVNQTKRGVEIDISASTLFQTGEASLQPGSQGVLRQVAAVLS
                     KEDFPVEVEGHTDDVPIATPQFPSNWELSSARASSVVRVLIEDGVPESRLAVVGLAAN
                     QPLAPNDSAENRAKNRRVTITILSSGLERDAASAN"
     gene            complement(7652..8122)
                     /locus_tag="VFW59_00310"
     CDS             complement(7652..8122)
                     /locus_tag="VFW59_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEX5362689.1"
                     /translation="MQNSSALLTALSAELSALQAFVTLLEREQNMLMDNQTDQLIELA
                     EQKASDAIKLNQIAESRIGLLQQHIPLLNAKTIESWLSQNQPEALPIWQVISDRTQHA
                     LQLNQTNGELIQMKLRHNQQALTVLSNAVNKVGVYGRNGQPNFSPGSGRSLGSG"
     gene            complement(8127..8435)
                     /gene="flgM"
                     /locus_tag="VFW59_00315"
     CDS             complement(8127..8435)
                     /gene="flgM"
                     /locus_tag="VFW59_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEX5362690.1"
                     /translation="MKIDKTSTPLPSTPVGEGRTNAAQKNSANPAPLQTASTSVSLGS
                     AATHLRNMEETMSGSSPVNAAKVAEIKQAISEGRFRINSDVVADRLISSVRELIGNKA
                     "
     gene            complement(8550..>8845)
                     /gene="flgA"
                     /locus_tag="VFW59_00320"
     CDS             complement(8550..>8845)
                     /gene="flgA"
                     /locus_tag="VFW59_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEX5362691.1"
                     /translation="IEMSHPSGFTHPNQVIGKVLRYSIAGGQVFRDDMLRAAYSVTQG
                     QNVQIISQGSGFSVRNEGAALNNASEGQRVQVRVGSGRVISGIARNGAVEVNP"
BASE COUNT         1701 a         2260 c         2966 g         1918 t
ORIGIN      
        1 gatggatgcg ctgatcacgt ttgccccgtt tctgctggct gtgatgatcg ccgcagcctt
       61 ttccccgttg ttgttgaatg gctggttgtt cagtatcggg gcgttgcagc caaaattctc
      121 caagttgaat ccgattgcgg ggatcgggcg catgttttcg cagacgtcgc tggttgagtt
      181 ggtgaaggcg ctggccaagg cgggattgat cggcggagtg tcggcctggg tgatctggaa
      241 caatcgggat gcggtgatgg cgctgggtat gcagtccgcc gcagcatcca ttcctgcggc
      301 cggtcatgtg atcggttcgg cctttatggt tatcgtcggg gcgatgttgt tgatcgtggc
      361 gatcgatgtg ccgttccagc tgtgggatca caacaagaaa ttaatgatga ccaaggaaga
      421 ggttcgtcag gaatcgaaag agtccgaggg cgatccgatg gtgaaagggc gcatccgtca
      481 gttgcagcgt gaggcagcgc gcaatcgcat gatgtccgaa attccgactg ccgatgtggt
      541 ggtgaccaac ccgacgcact acgcggtggc cttgaagtac agcgagaccg gcgcacgcgc
      601 gccggtcatc gtggccaagg gttcgcaatt gctggcgctg cgcatacgcg aactcgccgc
      661 agagcatcat gtgcctattc tggaagcgcc gccgctggct cgtgcgctgc acaaacatac
      721 cgaacttgga gagaccattc cggaggcgct atacaccgca gtcgccgagg tgctggccta
      781 tgtttatcaa ttgcgtcgtt acgagacagc gggcggggag cgtcccgtag agccggccga
      841 cttgccggtt cctgtcgagc tcgatccggc tgcgggcatc gtggcggcat gaacgaataa
      901 attatgaact ttcagctgat acaagatttc ctcaaacgcg caggcctggt tggtatggtc
      961 ggcccggtgc tgatcgtgct gattctggcg atgatggtat tgccgttgcc gccgttgctg
     1021 ctggacatgc tgttcacgtt caacatcgcg atctccatca tggtgctgct ggtcagcatg
     1081 aataccaaca agccgctgga cttcgcgatc ttcccgacgg tgctgctgat caccacgctg
     1141 ttgcgcttgt cgctgaacgt cgcctccacc cgtatcgtgc tgctggaggg gcacaccggc
     1201 ccggatgcgg cgggcaaggt gatcgagtcg ttcgggcact ttctggtcgg tggcaactac
     1261 gcggtgggtc tggtggtgtt cgcgattctg gtggtcatca acttcgttgt gatcaccaag
     1321 ggtgccgggc gtatcgccga ggtgggcgcg cgtttcacgc tggatgcgat gccgggcaag
     1381 cagatggcga tcgacgccga cttgaatgcc ggcctgattg gcgaggacgt ggcgcgcaag
     1441 cggcgtgaag agatcgctca agaggcggac ttctatggcg cgatggacgg tgccagcaaa
     1501 ttcgtgcgcg gcgatgccgt ggcgggcatc atcatcctgt tcatcaacat catcggcggt
     1561 ttgattgtcg gcgtgctgca gcatgacctc gacatcgcga cggcggcgaa gaactacacc
     1621 ctgctgacca tcggcgacgg actggtcgcg cagattccgg cgctggtgat ttctacggca
     1681 gccggtctgg tggtgagccg tgtggccagc gaagagaata tcggccagca gttgatcaag
     1741 cagttgttca tgcagccgca ggtgctcatc ctgacggcgg tgattatcgg cgcgttgggc
     1801 atgattccgg gcatgcctca ctttgccttc ctgttgctgg gcggtggcat ggcctggctg
     1861 gcttattatt tgataaatag cggcgccaag gcgattgtgc tcgaagcaga agaagccgct
     1921 gcaaccgccg cgacgatgcc cgtggtcagc gaatcgaccg aggcgagttg ggacgatgtc
     1981 gcccaggtcg atgtgctggg gatggaggtc ggttaccggc tgatcgcgtt ggtggacaag
     2041 acgcaggatg gcgacctgtt gcggcgcatc aagggcatac gcaagaaatt cactcaggat
     2101 atcggcttcc tgccgccgcc cgtgcatatc cgcgacaatc tggatttgcg cccgaattcc
     2161 tatcgcatca cgctcaaagg ggtcgaggtc ggtactggcg aggctttccc caacatgttc
     2221 ctggcgatca atccgggcaa tgcgagtggc gagctggcgg gtaatcccac ggtcgatccg
     2281 gccttcgggt tgcctgcggt ctggatcgag ggtgggttgc gcgatcaggc gcagttgatg
     2341 ggatacaccg tggtcgatcc gggtacggtg gtggcgactc acctgagtca gctgctcagc
     2401 acccgcgctg ccgagttgct cggtcgacag gaggttcagc agctgctcga tcatctcgcc
     2461 aaggtcgcgc cgaagctggt cgaggatctg gtgcccaaga cgctgccgct gggcgtggtg
     2521 cagaaggtgc tgcagaactt gctggacgaa ggcatgcata ttcgtgacat gcgcaccatc
     2581 gtcgagacgc tggctgaggg tgccacgcgc actcaggatg cataccagct caccagctgg
     2641 gtgcgagtag cgctgggccc ggccatcgtt cagcagttct atccgtcgat gcaggagttg
     2701 caggtgatcg gcatggacaa ggagcttgaa tcggtcctgc tgcaggcgat gcagtcgagt
     2761 cagaatggca tgggtatcga gcctgggctg gcggataccg tgctgcgcga ggcgcgtgcg
     2821 gcagccgatc agcaggaaca gatggggctg cccagcgtga tgctggtgcc gacgccgttg
     2881 cgcgacttgt tggcgcgttt cctgaagcgg gcggtgccga accttagagt gatttcgcat
     2941 gatgaagtac cggattccaa gacgatccgg gtgaccgcaa tggtaggagg tagagcatga
     3001 gcattagaaa attcttcgcc ccgacagcac gtcaggccct gaaagaaatc cgcgacgaac
     3061 tggggccgga cgcgatgatt ctgtcgaatc gcaagacgga gagcggcgta gagatcatcg
     3121 cgctggcgca tggtgaaatc gcgaatctca ccggacaggc ggtcgcccag aaggcagagt
     3181 ggggcggtgg catcacggca gacgagtggg cccgttcggt ggtcgatgac catgccgcaa
     3241 ggccggtatc gcaggccaga gtggcgcccg caccaagccc tcagttggca cagcaggcgc
     3301 ggaatgcgcc gcagttaaaa cagccgcctc agcaggcgca gaacgcaccg caactcaagc
     3361 cgcagcaggc catgcccgcg cggaaggcgg agccggtcgc ggtgtcgcgt cagcccggaa
     3421 aattgccgca acctgaaatg cagatgccgc ttcctgagcc ggttgcagtg ccggcgaatc
     3481 agcctgtggt gccggggctg gtcgcgcagg tgcaggaaat gctggtcaag gaaatcaagt
     3541 cgatgggcag catgctgcag cagcagattt cggcgatgca ctggagtgat gtgcagcagc
     3601 gcgatccgca acgcaccgca atgttgcgca gaatgctcag cgccgggttc agcaccgtgc
     3661 tggcacggca gatgctggac aggatgccgg ccaatggcgc tcagggcgag ccctgggtca
     3721 agcaggtgct gaagcgcaat ctgctggtgg ctggcgaagc gaacgacatc atcgcgaagg
     3781 gcggggtcta tgcgctgatc gggccgactg gcgtgggcaa gacgacgact acggcgaagt
     3841 tggcggcacg tgccgtggtg cgttatggcg cggacaaggt ggcgttgctg accacggaca
     3901 gttacaggat tgcggcttac gagcagctca agatatacgg caagatactc ggtgtcgcgg
     3961 tgcatgcggt caaggatgcc gacgatttgc gtctgactct ctcggcgctg cgccacaagc
     4021 atctggtgct gatcgatacc gtggggatgg ggcagcgtga ccagcgcgtg agcgatcagg
     4081 ccgacatgtt cgaggcggcg ggcgtgcaat gcctgttgat gatgaatgca accagcagtg
     4141 gcgatacgct ggaagatgtg gtgcgcatgt accacaacga caaggtgatc ggctgcatcg
     4201 cgaccaagct ggatgaggcg gtcagctacg gtgccgtgct ggatgtggcg gtgcgccacc
     4261 ggctggtcat gcactacatg gcgaatggtc agcgtgtgcc ggaggatctg cacacgatca
     4321 atctcgatta catgttgcat cgggccttta aaccactgga ggaaaagact ccctttaccc
     4381 tgtgcgatct tgacttgccg gtgatgatgt ctgcggcaga gtctggcaag gaggccagtc
     4441 ttgcgctctg atggggtggt ggatcaggcg gaggggctgc gtcgcttgtt ggtcgcgaat
     4501 cagactcaga tcgtgaccgt ggtggcaggc aagaccggga tggggcgcac cagcgcaacc
     4561 atcaatcttg ctgcggcgct ggtgcgttcc ggcaaggatg tgctggtgct ggacgagaat
     4621 cacgcgccca ataatctgct ggactatctg ggcttgaccg ctcgccagga tttgctggat
     4681 gtggctcagg ggggctgtct gccgcatgag gcggtgctgg atgggcatgg ctttggtgtg
     4741 ttgccggcat cgcgtgccat acatgcgctt gcgatctcgc gccggtctgc cggaagcggt
     4801 ggggggctgg ggcgagacga gtggcggcgt atggaggcgg cgctgaccga ggtcagtcgc
     4861 ggcgtggatg tgatgctggt cgatggcgca atgctggctg gtcaggctgc ggcagtgtca
     4921 tcgagcctgg cggacggtgc gggcttgttg gtggtggtcg aggctacgcc cgagggtatt
     4981 accgatagct atgctctgat caagcgactg gcgctggaga attcgcgctt gcagtttgat
     5041 attgtggtga acaaggtcgc cagcgagaag gctgcgatga cggtgtttga caatatgtcc
     5101 aaggtggcac gcagcaaact ttcggttcgc ttggagtatc tgggatacgt tccgctcgat
     5161 gaaaagataa ggtatgcgac ccagatgggg aaatcggtgc tggatgcgtt tcctgcgacg
     5221 gcctcggcga aggcttatct ggagttggcg cagaacctgt tgcgtcagcc gatgcggaag
     5281 gatgaggcgg agagcggggt cgcggccatc atacatagcc tgatgagcca agtgtcgcag
     5341 ccgctgcgcg gatacagcaa ggaaatcgct cacgttgtaa actagcagca tgtataaggc
     5401 taccggatta aaggataaag aacattgcct gcgggaatac tcgcatctgg tgaagcgtat
     5461 cgcgcatcag atgatgacca ggttgccgca tagcgtgcag atcgacgata tcattcaggc
     5521 gggcatgatc ggtctgctgg atgctgccag tcgttacgat gtgtttcacg gcgcgcagtt
     5581 cgaaaccttt gcttcgcaac gtatacgagg tgcgatgctg gatgagttgc gcgatgcgga
     5641 ctggttgccg cgcagtctgc gccgcgatat gcgacgcatc gagcagtcca tcagccgctt
     5701 gcagcagaag ttagggcgag cgcccaacga gggtgagatc gctgccgacc tggaagtccc
     5761 cctccccgaa tatcagcaaa tgttgcaggc gtcgcgcggc gcgcaattgc tgtattacga
     5821 agattttcat gacgaggatc acgacgattt tttcgaacga ttcgaatttt ccagcgacga
     5881 taatccgctg gagttgctgt ccaacgagcg attcagggcc gaactggtga gcgcaatcga
     5941 tagcttgccg gagcgtgagg ccatggtgat ggcgatgatc tatgagcagg atatgaactt
     6001 gcgtgagatt ggtgaagtgc tcggtgtggg cgagtcgcgc gtctcgcaat tgcatagtca
     6061 ggcggtgggt cgcctgcgca gccgcatgaa gggctattga tggataagct caccttgctt
     6121 ggattgctgg tcggtgtgac gggaattttg accggacagg tgttggaggg ggggagcctt
     6181 tccattctgt tgcagggggc ggccttcgtg atcgtgttcg gcggaacgct gggcgccgtg
     6241 atggtgcaat ctccgtccaa ggtgttcatt accgcaatca agatggggcg ctgggcattc
     6301 aagcctccac aggcgaacgt ggacgaattg gtgcatcagt tgtcaatgtg gggacaggtg
     6361 gcgcgcaagg aaggaattct ggcgctcgaa gtgcgtgtgg gtgtggttga ggatccgttc
     6421 cttaaaaagg gattgcagtt gctggtcgac gggaacagcg gcgataagat acgcgaagtg
     6481 ctggatgtgg atatccactc gtgggagcag ttgcgctggc aagcggcgcg ggtatgggag
     6541 gctgcggccg gttattcgcc gactatcgga attatcggtg cggttcttgg tttgatgcat
     6601 gtcatgcaga atctggcaga gcccagcaag ctgggcgcag gtattgcggt ggcattcgtg
     6661 gcgacgatct atggtgtcgg ttttgccaac ttgctgtttc tgcccatagc caataaatta
     6721 aagtctatcc tgatgcagca gtctgagctg cgcgagatga ttgtggatgg tctggtcggg
     6781 atcgcgaacg gggagaatcc aaggttgctc gagatcaaat tgcgcagcta tatcgattag
     6841 tttggtcccg ctcttggcgg ggttattttc agggaatcat catggcgcgt cgccgcaaac
     6901 gtctcgaaca tgacaaccac gatcgctggc tgatctccta tgccgatttt gtcaccttgt
     6961 tgtttgcttt ctttgtggtc atgtactcca tttcctcggt gaatgaggaa aaatacaaga
     7021 catttggcac tgccttgaat agtgccttcg gcaaacaggc cggcacgatt tcaccttccg
     7081 agaagagtaa tccgcaggag cagagcattt tgctcatggt tgatccgcag acggctcgtc
     7141 tgggagcgca gcagcgcaag attcaggaac acatgaaaag cctgcatgcg gggttgagtc
     7201 aggtgatggc ggatttgatt tctcagcgtc aggttagcgt taatcagacc aagcgcggcg
     7261 tggaaatcga tattagcgcg agcacgctgt ttcaaacggg cgaggcctcg ctacagccgg
     7321 gttcgcaggg tgtgttgcgt caggtggctg ccgtcttgag caaagaagac tttcccgtcg
     7381 aggtggaagg tcacactgat gatgtcccga ttgcgacgcc gcagtttccc tccaattggg
     7441 agttgtcttc tgcgcgcgcc agcagtgtgg tgcgtgtgct gatcgaggat ggtgtgccgg
     7501 agtcgcggct cgcggtcgtc gggctggcgg cgaaccagcc attggcgcct aatgatagcg
     7561 ctgagaatcg agccaaaaac aggcgcgtaa ccatcaccat cttgtcttcg ggtttggagc
     7621 gcgacgccgc gagcgccaat tgaggcgcag gtcagccgct tcccagcgag cggccgctgc
     7681 caggggagaa gttcggttgt ccgttgcgac cgtagacgcc aaccttgttg acggcattgc
     7741 tcaaaaccgt cagtgcttgc tggttgtggc gcaacttcat ctgaatcagt tcgccgttcg
     7801 tctggttaag ctgcagggca tgctgcgttc tatcgctgat gacctgccag atgggcaagg
     7861 cttcgggctg gttctggctt aaccaggatt cgatcgtttt ggcgttcagc agggggatgt
     7921 gctgctgcaa taggccgatg cgcgactccg cgatttgatt gagtttgatg gcgtcgctgg
     7981 ccttctgttc ggcgagctcg atcagctggt cggtctggtt gtccatcaac atgttctgtt
     8041 cgcgctcaag cagcgtaacg aaggcctgca gtgcggagag ctctgcggac agtgcggtca
     8101 gcaaggctga agagttttgc aaaatatcag gccttgttgc caatcagttc gcgtacggag
     8161 gagatcaggc ggtcagcgac gacgtcggag ttgatcctga aacggccctc gctaatggcc
     8221 tgcttgattt cggccacttt tgccgcattg actggcgagc tgccagacat ggtctcttcc
     8281 atgttgcgca gatgcgtggc cgccgagccc agcgatacac tcgtgctggc cgtctgaaga
     8341 ggcgcagggt tcgcgctgtt tttctgtgcg gcgtttgtcc tgccttctcc gactggcgtg
     8401 gatggtagcg gggtgctggt tttatcgatt ttcacggtag tcctctcaat ctggcgacgc
     8461 cgatatccgg gtcacggtct atttatcggc aaaaacattt agaactttag ggtgctcgta
     8521 atataaacgc aagtggcatg ctgcaccgct catggattga cctcgacagc accatttcgc
     8581 gcgatcccgc taattactcg gcccgacccg acgcgaacct gaacgcgctg tccctcgctg
     8641 gcgttgttca gcgcggcacc ctcattgcgt acgctaaacc ctgagccttg cgaaataatc
     8701 tgaacattct gcccctgagt gacgctatag gccgcgcgca gcatgtcgtc acgaaatacc
     8761 tgcccgccgg cgatgctgta tcgcagcacc ttgccgatga cctggtttgg atgcgtgaag
     8821 ccggaagggt gtgacatctc gatgg
//
LOCUS       DASUIV010000011        28442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_188159, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000011 DASUIV010000000
VERSION     DASUIV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 28442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28442
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188159"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFW59_00325"
     CDS             <1..358
                     /locus_tag="VFW59_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone"
                     /protein_id="HEX5362692.1"
                     /translation="KPAAKKVVAKKVAAKKVAAKKVAAKPAAQKVAAKPAVKVAAKPA
                     AKKVVAKPAAKVAAKPAAKKVVAKPAAKVPAKPAAPAVVVAKPVVSPAPAPSSPAPVR
                     PAATWPFPTPGIGKPN"
     gene            727..2058
                     /gene="pabB"
                     /locus_tag="VFW59_00330"
     CDS             727..2058
                     /gene="pabB"
                     /locus_tag="VFW59_00330"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX5362693.1"
                     /translation="MSCFYSAELPYQSDASIYYAAIADLPWAMWLDSGGMARYDILTA
                     APQHTIELDESSSCGDPFTLLRGKLGALVDAVDGVPFAGGALGYWSYDLARQAMNLPD
                     CALDAEHLPSMAVGLYDWALVLDHQQKTARVVSHRRYAETDALLPKILQRLQGGASRL
                     MDKFQVCGRIESNFTQASYRAAFDVVQGYLQAGDCYQINLAQRFSAAAQGDAFGAYLE
                     LRRLSPAPYSAFLNLPQVQILCASPERFLSLRAGHVETKPIKGTRPRGGDIERDRQLV
                     DELRRHPKDRAENLMIVDLLRNDLGKSCVPGSVRVPKLFEVESYANVHHLVSTVEGEL
                     AAEHDALDLLRDCFPGGSVTGAPKRRAMQIIEQLEPHRRGVYCGAIGYIGFDGDMDTN
                     IVIRTLVYSQNRIRCWAGGGLVADSQCEAEYQETLDKASAMLGMLRQFGGS"
     gene            complement(2073..4187)
                     /locus_tag="VFW59_00335"
     CDS             complement(2073..4187)
                     /locus_tag="VFW59_00335"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX5362694.1"
                     /translation="MADADLLIEEVSAYLKPQDIEHIREAIEFSRIAHYGQMRKSGEP
                     YVTHPIAVARILTTLHIDVQAVVAALLHDVVEDTAVTGDDIAEKFGKPVAELVDGLSK
                     LDRLKFETREDAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLESMEREKCERIAR
                     ETMEIYAPIANRLGLHDIYHELEDLSFKHLHPNRYAVLAKALKVARGNRREVVNKILD
                     AIRLRLEEQHIHADVTGREKNIFSIYQKMQSKSLAFAEVLDIYGFRVLVDDVASCYVA
                     LGALHGLYKPIPGKFKDYIAIPKVNGYQSLHTTLFGPFSTPIEIQIRTHEMHRIADAG
                     VASHWLYKSGHESINDLHQKTHQWLQELLESLSQSTDSSEFLEHLKVDLFPDEVYVFT
                     PKGMIMSLPRGATAVDFAYSVHTDIGNRCIAVKVNHELAPLRTELRSGDRVEVITAPH
                     AKPNPAWLGYVATSKARGHIRHFLKTMQSGESAQLGERLLNQALHTLGVKAQDMDEAH
                     WNRLLRDTGVKTREDILADIGLGRRLNMVVARQLASIGESAPGEIAANAVVTIQGTEG
                     MAVQFAKCCCPIPGDPVIGVIKSGQGLVVHTHDCPSLRRGRSGSEQWLDVVWDKNISR
                     SFEISIKLIVANQRGVLAKIAAAISDAESNISNVNFINEGEYTALNFTLEVNNRLHLA
                     NIMRSLRKVPEVVRIIRVKNAG"
     gene            complement(4205..4411)
                     /gene="rpoZ"
                     /locus_tag="VFW59_00340"
     CDS             complement(4205..4411)
                     /gene="rpoZ"
                     /locus_tag="VFW59_00340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5362695.1"
                     /translation="MARITVEECLTHIPNRFELTLAAAYRARQLANGATHLVEESKDK
                     PTVIALREISEGKVGKEILNRGQA"
     gene            complement(4415..5032)
                     /gene="gmk"
                     /locus_tag="VFW59_00345"
     CDS             complement(4415..5032)
                     /gene="gmk"
                     /locus_tag="VFW59_00345"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5362696.1"
                     /translation="MSGNLFIVSAPSGAGKTSLVHALLGINPQIDLSISYTTRNPRPG
                     EQDGKDYNFVSRETFLAMMKGGEFLESAEVYGNFYGTSQNWIAQEIDKGRDILLEIDW
                     QGATQVRKLFPHSISIFILPPSLQALEQRLKGRGKDNEEIIARRMAAVRDDVAHVAEF
                     DYVIINDSLNEALRELNSVVLAAKVRAGSQLARHQELINQLQNPE"
     gene            complement(5041..5919)
                     /locus_tag="VFW59_00350"
     CDS             complement(5041..5919)
                     /locus_tag="VFW59_00350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX5362697.1"
                     /translation="MILSMTGYANIGTEIDSGSLTLELRAVNHRYLDIQLRMPDELRG
                     FEGAMREAISAQLQRGKVECRINYAARTAQGGAALNRELLQQLAAWNDEVQRVLPDAR
                     SLNVADILRWNGVLETPSASADELRGALLNLLQEALQEFSASRAREGEKLKDFLLLRV
                     EKIEALRTGVMPHVPAAIAAYEQKLGARLREAMQTAVTQDLWDERIRQEITLFASKID
                     VDEELSRLDSHLTEMRRILTQGGAVGKRLDFLMQELNREANTLGSKSVDAEVSRSAME
                     MKILIEQMREQIQNLE"
     gene            6017..6808
                     /locus_tag="VFW59_00355"
     CDS             6017..6808
                     /locus_tag="VFW59_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5362698.1"
                     /translation="MKFSVHQASHIGNRKYNQDRVAYAYSSDALLLVLADGMGGHQHG
                     ELAATLIIDTFVESFGRHAQPRIAAPEQFIAEAMRHANERIMQVPHDKSAGGFPGSTC
                     VAALIQDGKMYWGHAGDSRLYLLRDGKVFAKTKDHSMVFQWMEWGVISAEEARVHPQR
                     NQITNCLGGQSDLFYMEPGEPVALQSGDLLLLGSDGLWGPFTDDELIKAFSVSSVGGL
                     DDLITRALERELGRSDNVTGVAARWGDDEVPHDTDEPVCHVLEIS"
     gene            6865..7584
                     /gene="rph"
                     /locus_tag="VFW59_00360"
     CDS             6865..7584
                     /gene="rph"
                     /locus_tag="VFW59_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5362699.1"
                     /translation="MRPSARSFNQLREIRITRSYTKHAEGSVLIECGDTKVICTASVE
                     ERVPPHKKGSGEGWVTAEYGMLPRSTGSRMSREAAKGKQSGRTQEIQRLIGRSLRAVV
                     DLGKLGERTIQIDCDVIQADGGTRTASITGAYVALADAVSWLKQQGLITESPLKDSVA
                     AISVGIYQGKPVLDLDYLEDSDCDTDMNVVMLGSGHFVEVQGTAEGHPFTRAEMDDLL
                     ELAKNGIAQLVEMQRAALATD"
     gene            7632..8222
                     /gene="rdgB"
                     /locus_tag="VFW59_00365"
     CDS             7632..8222
                     /gene="rdgB"
                     /locus_tag="VFW59_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX5362700.1"
                     /translation="MQKLVIASNNQGKLREFQRMLAPLGIEVLTQAELGISEAEEPYC
                     TFVENALAKARHVSRESGLPALADDSGICVDALGGAPGVQSARYAGDNPRSDQRNNEK
                     LLRELHGVTDRRAHYYCVLVLVRHADDPQPLIAEGEWHGEIAHEERGDGGFGYDPMFW
                     LPEFGKMSAELSHEEKALHSHRAKALKVLLQRLHVA"
     gene            8219..9412
                     /gene="hemW"
                     /locus_tag="VFW59_00370"
     CDS             8219..9412
                     /gene="hemW"
                     /locus_tag="VFW59_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5362701.1"
                     /translation="MTTHPLQPVGLKSQSVSFKALPPLSLYIHVPWCVRKCPYCDFNS
                     HEARGAFPEKEYVAALIRDLELALPQIWGRKVYSVFFGGGTPSLLSGESVAEILRQVR
                     MLLPLALDAEITLEANPGTVEAERFAAYREAGVNRLSLGIQSFNDDHLRALGRIHSGD
                     EAKRAIGIAQQHFDNVNLDLMYALPQQSLEQALLDVQTALSFSPQHLSCYHLTVEPNT
                     LFHHNPPSLPDDDASAEMQQRIEALLASYGYEHYETSAFARRRAKHNLNYWQFGDYLG
                     IGAGAHSKLSFPDRVLRQMRYKQPQAYLDAVGRDAPVQDEYAVAASELPFEFMMNALR
                     LNEGFDSVLFDERTSLPLMAMRRELEQAERRGLLIRDAQRIAPTLQGRRFLNDLLEIF
                     LRDAP"
     gene            complement(9461..9934)
                     /gene="trmL"
                     /locus_tag="VFW59_00375"
     CDS             complement(9461..9934)
                     /gene="trmL"
                     /locus_tag="VFW59_00375"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEX5362702.1"
                     /translation="MFQVILYQPEIPPNTGNIIRLCANTGCQLHLIKPLGFTLEDKQL
                     LRAGLDYHEFASLRVHDSLAECLATLATKRVFALTTKGATPFHQVTYQSGDAFLFGPE
                     SRGLPEEVLALFDSGHRLRLPMLPDNRSLNLSNTVAVTVFEAWRQCGFEGASGIS"
     gene            10032..11735
                     /locus_tag="VFW59_00380"
     CDS             10032..11735
                     /locus_tag="VFW59_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5362703.1"
                     /translation="MARPEKVRLGDLLVQQKFITLDQLKFVLDQQRISGRKLGRVLVD
                     NKLVTEEQISEALAKQLNIPYINLKFYNINHELVRLLPENQARRSRAIVLEERNGMLL
                     VGMADPTDLSAFDQVSSTLRRDIDLAVVTEGQLLESIDRGYRRTEEITGLARELSDDM
                     GDSYVDFGALVGTAGAEEAPVVKLLQTMFEDATQVRASDIHIEPQEARVVVRFRIDGL
                     LHLQMEADSKIAPALVLRLKLMSGLDISEKRLPQDGRFHVKVRDQGVDVRIATCPSQY
                     GESVVMRLLRQEVGMLGLDKLGMPPEMLARFRELIQRSSGMVLVTGPTGSGKTTTLYA
                     ALAEINTIEHKIITVEDPVEYRLSGIIQVQVNEKIELTFSRVLRSALRQDPDVILVGE
                     MRDAETAQIGLRAAMTGHLVFSTLHTRDTAGTLFRLVDMGVPRFMVASSVQVILAQRL
                     LRRICGSCAQPYQPSPQEAEWLQTEGLPPEQWGGISNGRGCSHCNGTGYRGRMGVYEM
                     LEMDREMTEAAAHESASHFMQVAREKMAGRSIINQALEQLRQGYTTITEVMRISSQVE
                     D"
     gene            11738..12991
                     /locus_tag="VFW59_00385"
     CDS             11738..12991
                     /locus_tag="VFW59_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5362704.1"
                     /translation="MAVFGYKGRNHGGELVTGSLEGDDSGSVADQLMSNGIIPLEITL
                     SHARVASAAASPVVDLPNRIWRKLNDKPVTPLDLMLFSRQMHTLLKAGVPILRALSGL
                     QESTQKPAFAAVIQDLRSSLDSGRELSSAMRQHPEVFSSFYLSMVQVGEMTGMLDSTF
                     MRLYEHMEFERDMRERIKAAMRYPSFVLLAMAIAIVIINLFVIPAFAKVFAGFHTELP
                     LMTRILIGFSDFMVNYWMLIFALVVGAVVAFRSYINTTAGRYRWDRYKFHLPIAGPII
                     FKSTLARFSRSLALSFKSGMPVLQGLDAVALVVDNAFMRSRIEGMRAGVERGESILRT
                     AMDAGVFNPMVLQMIAVGEETGDLDGLMFEVADMFQREVEYDVKNLSSQIEPILIVFL
                     GVLVLILALGVFLPMWDLGKAAMHK"
     gene            12988..13560
                     /locus_tag="VFW59_00390"
     CDS             12988..13560
                     /locus_tag="VFW59_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX5362705.1"
                     /translation="MSQSPRQQAVLPLWQRGAGGICQRGFTLIELIVVIVIVVVMAGA
                     FLGRVYYYQERAEKTAMEGVAASIQSALTMQYGHILIHGKPSDLPALAQGNPMNWLQK
                     KPGNYAGEFYEPTPQRVATGSWIFDLKTHDLIYVLNNANYFKPGKDGEKWIRFHVAIN
                     YDPPLPTASQNAPLELTGALFEPVELYSWF"
     gene            13597..14211
                     /locus_tag="VFW59_00395"
     CDS             13597..14211
                     /locus_tag="VFW59_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362706.1"
                     /translation="MSTPIVLEIILWRSLAFFLLVGAAVGIFVALLLIFRSELLGRIN
                     RVANRWVSTRHLDQALERSVSVEHWCYRHHQLLGALVCLGAVYIFAYFGLRFDKAFVM
                     AHLAGLLPGVLLDVLLDALVLTALTGAVASLYVGLLLWLRPSLLRGTEEVANQWLSTR
                     RAVRPLEIPRNQMDEFVARHAQRVGWWLLLGSLVLLVMVLRALV"
     gene            14361..14756
                     /locus_tag="VFW59_00400"
     CDS             14361..14756
                     /locus_tag="VFW59_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX5362707.1"
                     /translation="MQKQSGFTLIELIMVIVILGILAATALPKFVNLSADAKDAALKG
                     VAGGLASANVINVGGCAIAGSAVAGKCVKVAKCSDVGTLMEPTLTLGTTTSTTVPYLA
                     ADTAVTAGSSVSCTVQQDAASAVYAATGT"
     gene            14895..15413
                     /locus_tag="VFW59_00405"
     CDS             14895..15413
                     /locus_tag="VFW59_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5362708.1"
                     /translation="MLHPSSQIKEHAPESSRQGGFTLIELIMVLVIAGILAVFVAPRF
                     FDANIFKARGFADQAQAAMRYAQKIAIAQRRLVCVAVTAPTATSARLDLAIAVSNAAT
                     SCATPLALPASSSNFIQSSDATTSAANFVFDALGRPFALPYASGNASVGQTVTVAGYA
                     IKVEKETGYVHQ"
     gene            15499..16047
                     /locus_tag="VFW59_00410"
     CDS             15499..16047
                     /locus_tag="VFW59_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX5362709.1"
                     /translation="MFIVIVSVALAGIMLVMNVTTRSSADPLVHKQALAIAESLLEEV
                     ELMPFTICDPNDINAASAVTSADCTGGAGGAYDEARLPLGPETISGVVETRHSTTDPF
                     DNVSDYNGFDSVADGGIKDIAGNAVAGLGAYKATVSVAQQVIGGIPLPESSLITVSVT
                     GADGQAVVVEGVRARYAPRALP"
     gene            16044..17003
                     /locus_tag="VFW59_00415"
     CDS             16044..17003
                     /locus_tag="VFW59_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5362710.1"
                     /translation="MKRIVESGSALRASVERRCAPQWKEAPFGHSLRSSQFPLTTYHS
                     PLTSKGFTLVEMIMVIVITGIIGGIVAVFLKAPIQQYADVARRADMTDIADTALRRMS
                     RDLRLALPNSVRVSGACDGVGSCNLEFIPTHGGGRYRAGVGGGAGCAASPGGEELDFT
                     VADTCFGVLGTMPAMSAGDEIVVYNLGIAGADAYAGNTLASHNRRAASAAGNIVTLTS
                     VNALPFDSPGHRFHVVTTPVTYVCTPNALNPALGTLTRLSGYGWAAPAGAGDLLAKGV
                     GRCLFSYDPLVVGRSGVVTMQLAITEQGETVSLFSASHVSNQP"
     gene            17000..17509
                     /locus_tag="VFW59_00420"
     CDS             17000..17509
                     /locus_tag="VFW59_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362711.1"
                     /translation="MMKSRIEDRGLRVGGARGIAGLTCASLSAPRLSKGFSLVSAIFL
                     LVVIAALGAFAVTLSTTQQQSAALDVLGARGYQAARAGVEWGAYQVLRNPAGIACPVG
                     GASNPVAMPGAPSTLAIFAVDVSCWSYAPISEAAGTVTMYRLVSTATQGTPATPNYVE
                     RQMSVTIAQ"
     gene            17604..17816
                     /gene="rpsU"
                     /locus_tag="VFW59_00425"
     CDS             17604..17816
                     /gene="rpsU"
                     /locus_tag="VFW59_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5362712.1"
                     /translation="MTTVRVKENEPFEVAMRRFKRSVEKTGLLTDLRAREFYEKPTAE
                     RKRKLAAAVKRHYKRLRSQTLPPKMY"
     gene            17850..18296
                     /locus_tag="VFW59_00430"
     CDS             17850..18296
                     /locus_tag="VFW59_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5362713.1"
                     /translation="MSLKQQITDDMKAAMRAKEAARLGAIRLLLAAMKQREVDERIEL
                     SDADVVSIIEKMLKQRRDSISQYEAAGRQDLADVEKFEVGILQAYMPQQMDEAEINAA
                     IAEVVAATGAAGPQDMGKVMGVLKPKLAGRADMGRVSALIKAQLAK"
     gene            18414..20138
                     /gene="dnaG"
                     /locus_tag="VFW59_00435"
     CDS             18414..20138
                     /gene="dnaG"
                     /locus_tag="VFW59_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5362714.1"
                     /translation="MIPKSFIQDLLNRLDIVDVVERHVPLKKAGANYVACCPFHNEKS
                     PSFTVSQSKQFYHCFGCGAHGTAIGFVMEHVGLGFVEAVEELAQNAGMEVPHERAAAG
                     ETYQKVAPDLYEVMQVATRYYREQLKQSPRAIDYLKGRGLSGEVAARFGIGYAPEGWQ
                     SLAAAFPDYQSNALSETGLVIVNEEDHKRYDRFRDRVMFPITNVRGQVIGFGGRVLDK
                     GEPKYLNSPETPLFEKGRELYGLFQAQKAVRAAQRVLVVEGYMDVVALAQHGVDYAVA
                     TLGTATTPYHVQKLLRVVDHIIFCFDGDKAGQKAAWRALENALPHLQDGKRISYLFLP
                     VEHDPDSFIREFGHDAFEQRVHEAMPLSSYLLREISAELDLRSQEGRNQLLQRAKPLL
                     TAIAAPAAALLLRKEVAALAGVSQAELEALYEIRPVARPARPAPKKAGRAAPSGRRLL
                     LQCLIARPALGAKLPADWQGDGAEDEVIHVLLAALRETDYTLSGPALTQYFQGTMHER
                     AIAAAEADMLSWGDEFDVEAEFTGLMNSLDKDRRRQLFAGLQAKVAQSGLAGLSDAER
                     ECYRQAGR"
     gene            20241..22178
                     /gene="rpoD"
                     /locus_tag="VFW59_00440"
     CDS             20241..22178
                     /gene="rpoD"
                     /locus_tag="VFW59_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX5362715.1"
                     /translation="MAKPSDKKKLDKKSAEVLLQPVVDPQQDIEARRTRLKTLIVLGK
                     ERGYLTYAEINDHLPDMLEVEQIEGVVSMINDMGIQVYDVAPDAEALIMTDAAPAVAD
                     EDAAAEAEAALSSVDSEFGRTTDPVRMYMREMGTVELLTREKEIEIAKRIEEGLKHMI
                     QAISACPATIAEILALATKIENDQMRVDELIDGFVDVEAAEAEAAAEEEASEEESENE
                     EADDVDEEEDEAATAAAAAANLAQLKAEAMTRFAVIADQFTKMGKAYEKFGYRSKQYD
                     KCQSTISDELMNFRFTAKQVDALCETMRSLVEEVRRSERSIQDMCVTKSKMPRPHFIK
                     TFPGNEENLEWVAQELKAKKPYAEALARYQHAIVEQQQNLLNLEARVGIPIKDLKEIN
                     KQMTNGEARARRAKRDMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFE
                     YRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQIMQETGIEP
                     DVATLAIKMEMPEDKIHKILKIAKEPISMETPVGDDDDSHLGDFIEDQNTTVPVDAAV
                     YESLRGATKDILDSLTQREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKA
                     LRKLRHPSRSEKLKSFLDSEG"
     gene            22187..22263
                     /locus_tag="VFW59_00445"
     tRNA            22187..22263
                     /locus_tag="VFW59_00445"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22221..22223,aa:Ile,seq:cat)
     gene            22316..26062
                     /gene="hrpA"
                     /locus_tag="VFW59_00450"
     CDS             22316..26062
                     /gene="hrpA"
                     /locus_tag="VFW59_00450"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEX5362716.1"
                     /translation="MTAELSASALRRKARLDGLSAIEFPADLPVVARREDLARAIQEH
                     QVVIVCGETGSGKTTQLPKICLTLGRGVKGCIGHTQPRRVAARTVASRIAHELKTELG
                     GAVGFKVRFNDKVSADTSIKLMTDGILLAEIHRDPLLRGYDTLIVDEAHERSLNIDFL
                     LGYLKQLLPRRRDLKLIITSATLDADRFAKHFDAKVVEVSGRSYPVEVRYRPPQVDEE
                     GNTQDVPQAVSSALDELAAGGLRGDVLVFLPGEREIRDTAEVLRKHHPKGIEVLPLFA
                     RLSAAEQDKVFKLSSGVRRVVLATNVAETSLTVPNIGYVIDCGLARVNRYSIRQKVEQ
                     LHIEKISRAAANQRAGRCGRVMSGVCIRLYDEEGFAQRPEFTDPEIFRVSLATVILRM
                     SALDLGDIAEFPFIEPPSPRMIADGYQLLAELGAIDEQRTLTKTGHELAKLPLDPKIA
                     RLLLAGRQYHCLNEILIIASALSVQDPRERPLDRQEAADTAHKRFADERSDFLAYPKL
                     WAWYRQAVADKKSNRLMAEECRKNFLSPLRLREWHELHQQLHTQVAEMGMRENEQPAT
                     YEQIHKALLCGLLGNIGMKGVEGNEYLGARGIKFLVASNSVLAKKGAKWVMAAELIET
                     HRLYARCVARIEPEWLEETGDHLIKRHYYDPHWEKKAAQVVAYERSTLHGLLLNPKKR
                     VHYGPMNPAESREVFIRQALVGGEFNTQAPFFAYNQKLIADIEALEHKARRPDVLVDD
                     ELIFAFYDSRIPADIYNGAAFEHWRKEAERDNAKLLYLKKDDLMRHEAAGITTDQFPP
                     QLKIDNVSFALSYNFSPGKNDDGITLTVPLALINQVVSARCEWLIPGLLAEKVQQLIK
                     TLQQRLRRHLVPVPEFAAAFARDVPPSNTPLLQALARYIREQKQLDVPLDAFRLEQLP
                     QHLLMNFRVIDEHGRQLGMSRNFAQLHGELAPRVAPIAVVAAATEKGESTSEQRYTAW
                     SFGAFEETRTVQRAGQSVTLFNALVDEGDAVVLRAFDTRDAAMAAHRGGLSRLFMLLL
                     KEQVKYLEKNLPDAQRLGMLFMPFGTQQELQQQILATTFERCCLGDPLPTNEAEFNAR
                     GKEAKNRLLLIAQEIARLVATILTEHQALQKSLPQLKAHAQVAQDVRAQLEWLLHKRF
                     VRDTPYERLQHVPRYLKAINLRIEKLRTNPARDAQCVSQIQPLTQALQKLRQAQQGKP
                     DPRLEEFAWLMQELRVSLFAQELKTPVIVSVKRLQKMLESLRSN"
     gene            complement(26166..26241)
                     /locus_tag="VFW59_00455"
     tRNA            complement(26166..26241)
                     /locus_tag="VFW59_00455"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26206..26208),aa:Phe,seq:gaa)
     gene            complement(26360..28003)
                     /gene="groL"
                     /locus_tag="VFW59_00460"
     CDS             complement(26360..28003)
                     /gene="groL"
                     /locus_tag="VFW59_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110680.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5362717.1"
                     /translation="MAAKDVKFHDAARSKMIIGVNILADAVKVTLGPKGRNVVLDRSY
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQSIVQE
                     GMKFVAAGMNPMDLKRGIDKAVLTAVEELKKLSKPCTTSKEIAQVGSISANSDSAVGD
                     KIAEAMDKVGKEGVITIEDGSGLEDELDVVEGMQFDRGYLSPYFINNQDRQIAAMDNP
                     FILLHDKKISNIRDLLPILEQIAKSSRPLLIIAEDVDGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLEDIAILTGGTVIAEEVGLTLEKATLEDLGQAKRIEVGKDNTIIID
                     GAGKTEAIQSRIVTINKQMEESSSDYDKEKLQERKAKLSGGVAVIKVGAAPEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRAKAAVAGLKGDNHDQDAGITIVLRAMEAP
                     LRAIVANAGEEPSVVVNKVLEGKGSFGYNAGTGEYGDMLAMGVVDPTKVTRTALQNAS
                     SIAGLMLTTACMVAESPEDKPAGGMGGEMGGMGGMGGMM"
     gene            complement(28033..28323)
                     /locus_tag="VFW59_00465"
     CDS             complement(28033..28323)
                     /locus_tag="VFW59_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5362718.1"
                     /translation="MKIRPLHDRVIVKRMEEERKTASGIVIPDAATEKPDQGEIVAVG
                     NGKMGDDGKVRPMNVKVGDKVLFGKYSGQAFKMDGVELLQMREDDIIGVIEA"
BASE COUNT         5425 a         7999 c         9106 g         5912 t
ORIGIN      
        1 caagccagcg gccaagaagg ttgtagccaa gaaagtcgca gccaagaaag tcgcagccaa
       61 gaaagtcgca gccaagccag cggctcaaaa ggtcgcagcg aagccagcag tcaaggttgc
      121 agcgaagcca gcagccaaga aggttgtagc gaagccagca gccaaggttg cagcgaagcc
      181 agcagccaag aaggttgtag cgaagccagc ggccaaggtt ccagcgaaac cagcggcccc
      241 ggctgttgta gtggccaagc cagtggtttc ccctgcgcca gccccttcgt cgccagcacc
      301 tgttcgcccg gccgctacat ggccattccc tacaccgggg atcggcaaac cgaactgatt
      361 gggggcgtcc ctgcgtgatg gcgtgagcct tggtgcggtg cagtctgttt aaaaggctgc
      421 taagcattcg attgggtcat aagatgttga ccgaatcttt tgctggggcg cgggaggggc
      481 tgatgcatgg cagctcccgc aatctgttgt atcacaggtc ttccgttggt gcttaggcgt
      541 tagggagcgt tcgcaattac attgctcttt ggggcgcact gagtttgggt gcgtgcgcct
      601 ttttgttttg ccaatcaaat attggcgtgg tgtttcctgc catcaccgcc tgcgggcgct
      661 cgtgataggt tttgtgcccc ttgaattcct gccccgttta aatcccactc gcttccatat
      721 tctgcgatga gttgcttcta ttctgccgaa cttccctacc agtccgatgc cagcatctat
      781 tacgccgcca tcgctgattt gccgtgggcg atgtggttgg atagtggagg catggcgcgt
      841 tacgatattc tgactgccgc accgcaacat accatcgaac tcgatgaatc ctcttcatgc
      901 ggcgatcctt tcacgttatt gcgcggcaag ctcggtgcct tggtggacgc tgtggatggt
      961 gtgccgttcg cgggtggcgc gcttggttat tggagctatg atctggcgcg acaagcgatg
     1021 aatctgccgg attgtgcgct ggatgcagag catctgccga gcatggcggt cggtctgtat
     1081 gactgggcgc tggtgctgga tcatcagcag aaaacggcgc gagtcgtgtc gcaccgacgc
     1141 tatgctgaaa cagatgcgct gctgccgaaa attctgcaac gcctgcaggg tggggcttcg
     1201 cgattgatgg ataagtttca ggtgtgcggc aggatcgaat ccaacttcac gcaagccagt
     1261 taccgtgccg ccttcgatgt cgtgcagggt tatctgcagg ctggcgattg ttatcaaatc
     1321 aacttggcgc aacgctttag tgcggcggca cagggggatg cattcggtgc ctatcttgaa
     1381 ttgcgccgac tgagtcccgc gccgtattca gcattcctca acttgccgca ggtgcagatt
     1441 ctgtgcgctt caccggagcg ttttcttagc ttgcgcgcag ggcatgtcga gactaagccg
     1501 atcaagggta cgcgtccgcg cggtggcgat atcgagcgtg atcgccagct tgtcgatgag
     1561 ttgcgtcgac acccaaagga cagggccgag aatctgatga tcgtcgacct gttgcgcaat
     1621 gacctcggca agagttgcgt gcccggctcg gtgcgcgtcc ctaagctatt cgaggtcgag
     1681 agctacgcta acgtgcatca tctggtgagt accgtagaag gagaattggc cgctgagcat
     1741 gatgcgctag atctgttgcg cgattgcttt cccggtggct ccgtgaccgg ggcgcccaag
     1801 cgccgcgcga tgcaaattat cgagcagctc gagccgcatc ggcgcggtgt gtattgcggc
     1861 gcgatcggtt acatcggttt cgacggcgat atggatacca atatcgtgat ccgcactctg
     1921 gtctattcgc aaaacaggat tcgttgctgg gcgggtggcg gcctcgtcgc ggactcgcaa
     1981 tgcgaggcgg agtatcagga aacgctggac aaggcctcgg cgatgcttgg gatgttgcgg
     2041 cagttcggcg gcagttaagt ctggttgccg tgctagccgg cgttcttcac ccgaatgatg
     2101 cgcaccactt cggggacttt gcgcaggctg cgcatgatgt ttgcgagatg caggcgattg
     2161 ttaacttcta gcgtgaaatt caaagcggtg tattcgccct cgttgataaa gttcacgttg
     2221 ctgatgttgg attccgcatc ggaaatcgcg gcggcgattt tggccagtac gccgcgctgg
     2281 tttgcgacga tgagcttgat gctgatctcg aaggagcgcg agatgttctt gtcccacacc
     2341 acatccagcc attgttcgct gccgctgcga ccgcgccgca aggaggggca gtcgtgggtg
     2401 tgcaccacca gcccctgccc gctcttgatg acaccaatga ccgggtcgcc cgggataggg
     2461 cagcagcact tggcaaactg gactgccatg ccttccgtgc cctgaatggt gacgacggca
     2521 ttggcggcga tttccccggg agcggattcg ccgatgctgg ctagttggcg ggccaccacc
     2581 atgttgaggc ggcgaccgag gccgatgtcg gcgaggatgt cttcccgcgt cttgacgccc
     2641 gtgtcacgca acagtctgtt ccagtgcgct tcgtccatgt cctgtgcctt gacgccgagc
     2701 gtgtgcagcg cctgattgag caggcgctcg ccaagctgag ccgattcacc cgactgcatg
     2761 gtcttgagaa agtggcggat atggccgcgc gccttgctgg tggcgacata gcccagccac
     2821 gcaggattcg gtttcgcgtg cggcgcggtg atgacttcca ctcggtcgcc gctgcgcagc
     2881 tcggtgcgta gcggagcaag ttcatgattg accttgactg cgatgcagcg attgccgata
     2941 tcggtatgca cgctataggc aaagtccact gccgtcgcgc cgcgcggcaa agacatgatc
     3001 atgcccttgg gtgtgaacac atagacctcg tccgggaaca ggtctacctt gaggtgttcg
     3061 aggaattccg acgagtccgt gctctgactc aggctttcca gcaactcctg cagccactgg
     3121 tgggtcttct ggtgcaggtc attgatggac tcgtgaccgc tcttgtaaag ccagtgcgag
     3181 gcgacacccg catcggcgat gcgatgcatc tcgtgggtgc gaatctggat ctcgatcggc
     3241 gtgctgaacg ggccgaacag cgtggtgtgc agcgactggt agccattcac cttggggatc
     3301 gcgatgtaat ccttgaactt gccagggatg ggtttgtata gcccgtgcag cgcacccagc
     3361 gcgacgtagc aggaggcgac atcgtctacc agcacgcgga atccgtagat gtcgagcacc
     3421 tccgcaaagg ccagcgactt gctctgcatc ttctggtaga tgctgaagat gtttttctcg
     3481 cgtccggtca catcggcatg gatgtgttgt tcctcaagtc gcaggcgtat cgcatcgaga
     3541 attttattga cgacctcgcg gcgattgccg cgcgcaacct taagcgcctt tgccaatacg
     3601 gcatagcggt tcgggtgcaa gtgtttgaag ctcaggtctt ccagttcatg gtagatgtcg
     3661 tgcaggccga gacggttggc gatgggcgcg tagatttcca tggtctcgcg cgcgatgcgc
     3721 tcgcacttct cgcgctccat cgactccaga gtgcgcatgt tgtgcagacg atccgccagc
     3781 ttgatcagga tgacgcgaac gtcgcgcgcc attgccagca gcatcttgcg gaagttttca
     3841 gcctgtgcgt cctcgcgcgt ctcgaatttg aggcgatcga gcttgctcag tccgtcgacc
     3901 aactctgcga caggtttgcc gaatttttcc gcgatgtcgt cgccagtgac cgcagtgtct
     3961 tcgaccacgt cgtgcaacag cgccgccacc accgcttgca cgtcaatgtg cagggtggtc
     4021 aggatgcgtg caacggcgat gggatgagtg acgtacggtt cgccggattt gcgcatctgg
     4081 ccatagtgcg caatacggct gaattcgatg gcctcgcgga tgtgttcgat atcctgcggt
     4141 ttaagatacg cagagacttc ctcgatcagt aaatccgcgt cggccatgaa aaaggatcag
     4201 taacttatgc ctgaccgcga ttcagaattt ccttgccgac cttgccttcg ctgatctcgc
     4261 gcagcgcgat gacggtaggc ttgtctttgc tttcctcgac cagatgggtt gcgccgttgg
     4321 ccagttggcg tgcgcggtag gcggcagcca gcgtcagctc aaagcggtta gggatatggg
     4381 tgaggcattc ttcgacggtg atacgagcca tgatttactc cggattttgc agttggttga
     4441 ttaattcctg atggcgagcc agttgggagc cggcgcggac tttggcggca agcacgaccg
     4501 agttcagttc gcgcaatgct tcgttcaggc tgtcgttgat aataacatag tcgaattcgg
     4561 cgacatgggc gacatcgtcg cgaaccgccg ccatgcgtct ggcgatgatc tcctcgttgt
     4621 ccttgccgcg ccccttcagg cgctgctcca gcgcctgtaa cgaggggggc aggatgaaga
     4681 tgctgatgga atgcgggaat agcttgcgca cctgtgttgc gccttgccag tcgatttcca
     4741 gcaggatgtc gcgccccttg tcgatttcct gcgcgatcca gttctgcgag gtgccgtaga
     4801 agttgccgta gacttccgcg ctctccagaa actcgccccc tttcatcatc gcgaggaaag
     4861 tctcgcggct gacgaagttg taatccttgc catcctgttc gccggggcgc gggttgcggg
     4921 tggtgtagga gatcgacagg tcgatctgcg gattgatgcc gagcagggcg tgcaccagac
     4981 tggttttccc cgcaccggac ggcgcgctga cgataaacag gtttccggac atgattagat
     5041 ttactccagg ttttgaattt gttcgcgcat ctgctcgatg aggatcttca tctccatggc
     5101 actgcgcgag acttcggcat cgactgattt cgagccgagc gtattggctt cgcggttcag
     5161 ttcctgcatc aggaaatcca gccgcttgcc gactgcacca ccctgagtca ggatgcggcg
     5221 catctcggtc aggtggctgt ccagccggga cagctcctcg tctacgtcga tcttgctggc
     5281 gaatagcgtg atctcctgac ggatgcgctc gtcccatagg tcctgagtga cggcagtctg
     5341 catcgcttcg cgcaggcggg cgccgagctt ctgttcgtag gccgcgatgg cggcaggcac
     5401 atgcggcatc acgccggtgc gcaatgcctc gatcttttcc acgcgcagca gcaggaagtc
     5461 tttcagtttc tcgccttcgc gggcgcgcga ggcggagaat tcctgcagcg cctcctgtaa
     5521 caggttgagc agggcgccgc gtaattcgtc cgccgaggcg ctgggtgttt ccagtacgcc
     5581 gttccagcgc agtatgtcgg ccacgttcag gctgcgtgcg tccggcagga cgcgctgtac
     5641 ctcgtcgttc caggcggcca attgttgcag caactcgcgg ttgagcgccg cgccgccttg
     5701 ggccgtgcgc gccgcgtaat tgatgcggca ttccaccttg ccgcgctgca gttgggcgga
     5761 gatcgcctcg cgcatcgctc cttcgaagcc gcgcagttcg tccggcatgc gcagctggat
     5821 atcaagatag cggtgattga cggcgcgcaa ctcaagcgtc agcgagccgc tgtcgatttc
     5881 tgtaccgata ttggcgtagc cggtcatgct caggatcatg gcgatagttc cgggaaaaaa
     5941 gggcagccga acaaagggct gtgctgaatg cgtgcattat attacgattg gcatccgtaa
     6001 attaaatatc cggcccatga agttctcggt gcatcaggcc agtcacatcg gcaatcgcaa
     6061 atataatcag gatcgcgtcg cctatgccta tagcagcgac gccttgctgt tggtgctcgc
     6121 cgacggcatg ggcgggcatc agcatggtga gctggcggca accctgatca tcgatacctt
     6181 cgtcgaatcg ttcggccgtc atgcgcaacc gcgcatcgca gcaccggagc aatttatcgc
     6241 agaggccatg cgccatgcta acgagcgcat catgcaggtg ccgcatgaca agagtgcggg
     6301 cggcttcccg ggatcaacct gcgtggcggc gctgattcag gatggcaaga tgtactgggg
     6361 ccatgcgggc gactcccggc tgtacctgtt gcgtgacggt aaagtctttg ccaagactaa
     6421 agatcattcc atggtgttcc agtggatgga gtggggcgtg atctccgccg aggaggcgcg
     6481 cgtccatccg cagcgcaatc agatcaccaa ttgcctgggc ggtcaaagcg acctgttcta
     6541 catggaacct ggcgaaccgg tcgcgctgca atccggtgac ttgctgttgc tcggcagcga
     6601 tggtctgtgg gggccgttta cggacgacga gttgatcaag gcattttccg tcagctcggt
     6661 cggaggattg gacgacttga tcacgcgggc gctggagcgc gagcttgggc gttctgacaa
     6721 cgttaccggc gtggcggcgc gctggggcga cgacgaagtc ccacatgaca ccgacgagcc
     6781 ggtctgtcac gtcctcgaaa tttcctgatc tgtagggggc gcgccgtgcc cctatcccct
     6841 gaatttttta tggagaaccg caacatgcgt cctagtgcaa gatcgttcaa ccaattgcgt
     6901 gaaattcgca tcacccgcag ctacaccaaa catgccgaag gttcggttct gatcgagtgc
     6961 ggcgacacca aggtgatctg taccgccagt gtcgaagagc gcgtgccgcc gcacaagaag
     7021 ggcagcggcg agggatgggt cacggcggaa tacggcatgc tgccgcgttc caccggcagt
     7081 cgcatgagtc gcgaagccgc caagggcaag cagtcgggcc ggacgcagga gattcagcga
     7141 ttgatcggcc gcagcctgcg tgcggtcgtc gatctcggca agctcggcga gcgcaccatc
     7201 cagatcgatt gcgacgtgat tcaggccgac ggcggcacgc gcactgccag catcaccggg
     7261 gcctatgtcg cgctggccga tgcggtaagc tggctgaagc agcaaggcct gatcaccgag
     7321 agcccgctga aggattccgt agccgcgatc tcggtcggca tctatcaggg caaaccggta
     7381 ctcgatctcg attatctcga agactccgat tgcgacaccg acatgaacgt ggtgatgctg
     7441 ggcagcggtc attttgtcga ggtgcagggc accgcagaag gccacccctt cacccgtgca
     7501 gagatggatg atctgctgga gctggccaag aacggtatcg cgcaactggt cgaaatgcag
     7561 cgcgcggcgt tggcgacgga ttgatatcaa aaggtagggg cacggcgcgc cgtgccccta
     7621 cgagtgaaac catgcaaaaa ctcgtcatcg catccaacaa tcagggcaag ctgcgcgaat
     7681 ttcagcgcat gctggcgccg ctcggcatcg aggttttaac gcaggcggaa ttggggatca
     7741 gtgaggctga agagccgtac tgcaccttcg tcgagaatgc gcttgccaag gcgcgtcacg
     7801 tcagtcgcga aagcgggctg cccgcgttgg cggacgattc cggtatctgc gtcgatgcct
     7861 tgggcggtgc gccgggcgtg cagtcggctc gctatgccgg ggataatccc aggtccgatc
     7921 agcgcaacaa cgagaagctg ctgcgcgaac tgcacggcgt gaccgatcgc cgcgcgcatt
     7981 actactgcgt gctggtgctg gtgcgccatg ccgacgatcc gcagccgttg atcgccgagg
     8041 gcgaatggca tggcgagatc gcccatgagg agcgcggcga cggcggcttc ggttacgacc
     8101 cgatgttctg gctgccggaa ttcggcaaga tgagtgccga gctgagtcac gaagaaaagg
     8161 cgttgcatag tcaccgcgcc aaggcgctca aggtgttgct gcagcgactt catgtcgcat
     8221 gaccacgcat cccctgcaac ccgtcggtct caaatcgcag tccgtgagct tcaaggcgct
     8281 gccgccgctg tcgctgtata tccatgtgcc ctggtgcgtg cgcaagtgtc cctattgcga
     8341 ctttaattcg catgaggcgc gcggcgcttt ccccgaaaaa gaatatgtcg cagccttgat
     8401 ccgcgatctg gaattggcgt tgccgcagat ctgggggcgc aaggtttata gcgtgttctt
     8461 cggtggcggc acgcccagcc tgctgtcggg ggaaagtgtc gcggaaattt tgcggcaggt
     8521 gcgcatgttg ctgccgttgg cgctcgatgc cgagatcacg ctggaggcga atccgggcac
     8581 ggtggaggcg gagcgttttg ctgcctatcg cgaggcgggg gtgaaccggc tgtcgctggg
     8641 catccagagt ttcaatgacg atcacctgag ggcactcgga cgtatccatt cgggagacga
     8701 ggcgaagcgc gcgatcggaa tcgcgcagca gcacttcgac aacgtcaatc tcgatctgat
     8761 gtacgccttg ccgcagcagt ctctggagca ggccttgctc gatgtgcaga cggcgctatc
     8821 cttttcgcct cagcatctgt cctgctacca cctcacggta gagcccaaca cgctgttcca
     8881 tcacaacccg ccgtccttgc cggatgacga tgccagcgcc gagatgcagc agcgcatcga
     8941 ggcgctgctg gcatcgtatg gctacgaaca ttacgagacc tcggcctttg cgcggcgacg
     9001 cgccaaacat aatctcaatt actggcagtt cggcgattac ctcggcatcg gtgcgggagc
     9061 gcacagcaag ctgagttttc cggatcgggt gttgcgccag atgcgctaca agcaaccgca
     9121 ggcctatctc gatgcggtgg ggcgtgatgc gccggtgcag gatgagtatg cagtggcggc
     9181 gagtgagctg cccttcgagt tcatgatgaa tgcactgagg ctgaacgagg gattcgattc
     9241 cgtgctgttc gatgaacgca ccagcctgcc gttaatggcg atgcgccggg agctggagca
     9301 ggccgaacgg cgcgggttgc tgatccggga cgcccagcgc atcgcgccga cgctgcaggg
     9361 gcggcgattt ttgaacgacc tgctggaaat ctttctcagg gatgctccgt aggggcacgg
     9421 cgcgccgtgc ccctacgggt gaattgacac cgccgtgccc ctacgaaatg cccgacgcac
     9481 cctcgaatcc gcattgtcgc caggcttcga acacggtcac ggccaccgtg ttggacaggt
     9541 taaggctgcg gttgtccggc agcatcggca ggcgcaagcg atgcccgcta tcgaacagcg
     9601 ccagcacctc ctccggcagg cctcggcttt ccgggccgaa cagaaaggcg tcgccgcttt
     9661 ggtaggtgac ctgatggaag ggggtagcac ctttggtggt gagggcgaac acccgcttcg
     9721 tggccagcgt ggcgaggcac tccgccagac tgtcgtggac gcgcaggctg gcgaattcgt
     9781 gataatccag tccggcgcgg aggagttgct tgtcttccag cgtgaaaccg agcggtttga
     9841 tcaggtgcag ttggcagccg gtgttggcac acaggcggat aatgttgccg gtgttgggcg
     9901 ggatttccgg ctggtagagg atgacttgga acatgggaag tatgggtaat gcttgtcagt
     9961 gttgataaaa aaggctaagt tcgcgctcac atggctctcg atgcagaggc gatttatttc
    10021 agggaaagat tatggcgcgt ccggagaagg ttcgcttagg cgatttgctg gtacagcaaa
    10081 agttcatcac gctcgatcaa ctcaaattcg tactcgatca acaacgcatc agtggccgta
    10141 agctcgggcg tgtgctggtc gataacaaac tggtgaccga ggagcagatc tccgaggcgc
    10201 tcgccaagca gctcaatatt ccctacatca atctcaagtt ctacaacatc aatcacgagc
    10261 tggtgcggct gttgccggag aatcaggcgc ggcgttcgcg tgcgatcgtg ctggaggagc
    10321 gcaacggcat gctgttggtg ggcatggccg acccgacgga tttatccgcc ttcgatcagg
    10381 tctccagcac ccttcgtcgc gacatcgatc tggccgtggt gaccgagggg cagctgctgg
    10441 aaagcataga ccgcggttac cggcggactg aggaaatcac cggtctggcg cgtgaattga
    10501 gcgatgacat gggcgacagc tatgtcgact tcggtgcgct ggtcggcacc gccggggccg
    10561 aagaggcgcc ggtggtgaag ttgctgcaga ccatgttcga agatgccacg caggtgcgcg
    10621 cctccgatat tcacatcgag ccgcaggagg cgcgcgtggt ggtgcgcttc cgcatcgacg
    10681 gcctgttgca tctgcagatg gaggccgaca gcaagatcgc gcccgcgctg gtgttgcgcc
    10741 tgaagctgat gtccggtctg gatatctcgg aaaagcgact gccgcaggac gggcgcttcc
    10801 atgtgaaggt gcgcgatcag ggcgtggatg tgcgtatcgc cacttgtcct tcacagtatg
    10861 gcgaatcggt ggtgatgcgc ctgttgcgtc aggaagtcgg aatgctgggg ttggacaagt
    10921 tgggcatgcc gccggagatg ttggcgcgct ttcgcgaact catccagcgc agcagcggta
    10981 tggtgctggt gaccggtccg accggcagcg gcaagaccac cacgctgtat gccgcgctgg
    11041 cagagatcaa caccatcgag cacaagatca ttaccgtcga ggacccggtg gaatatcgcc
    11101 tgtccggcat tattcaggtg caggtaaacg agaagatcga actgactttt tcccgggtgt
    11161 tgcgctcggc cttgcgtcag gatccggatg tgattctggt cggcgagatg cgcgatgcgg
    11221 aaacggcgca gatcggtctg cgtgcggcga tgaccggtca cctggtgttt tccacgctgc
    11281 acacccgcga tactgccgga accctgttcc ggctggtgga tatgggagtg ccgcgtttca
    11341 tggtggcctc gtcggtgcag gtcatcctcg cgcaacgttt gctgcgccgt atctgcggta
    11401 gttgtgctca gccgtatcaa ccttcgcccc aggaagcgga atggctgcag acggaaggtt
    11461 tgccgccgga acaatggggc ggcatttcga acgggcgcgg ctgctcgcat tgcaatggca
    11521 caggttatcg cgggcgcatg ggcgtgtatg aaatgctgga gatggatcgt gaaatgaccg
    11581 aggcggccgc ccatgagagt gccagtcatt ttatgcaggt cgcgcgcgaa aaaatggcag
    11641 gcaggagcat cattaatcag gcgctggaac agttgcggca gggctatacc accatcaccg
    11701 aagtgatgcg catcagcagt caggtagagg attagcggtg gcggtattcg gctataaggg
    11761 acggaatcac ggtggcgaac tggtcaccgg ctcgctggag ggggacgaca gcggatcggt
    11821 cgccgatcaa ttgatgagca acggcatcat cccgctggag atcacgttgt cgcatgccag
    11881 agtggccagc gctgcggcct cccccgtcgt tgatctgccc aaccggatct ggcgcaagct
    11941 caacgacaag cctgtcaccc cattggattt gatgctgttc agtcgtcaga tgcataccct
    12001 gctgaaggcg ggggtgccga tactgcgtgc gttgtccggt ctgcaggagt ctacgcagaa
    12061 gccggcattc gcggcggtga ttcaggattt gcgctctagt ctggacagtg gccgcgaatt
    12121 gagttcggcg atgcgccagc atccggaggt attttcttcg ttctatctca gcatggtgca
    12181 ggtcggtgaa atgaccggca tgctggatag caccttcatg cgcctgtacg aacatatgga
    12241 attcgagcgc gacatgcgcg aacgcatcaa ggcggcgatg cgttatccga gtttcgtgct
    12301 gctggcgatg gcgatcgcca tcgtgatcat taacctgttt gtgatccccg cctttgccaa
    12361 ggtgttcgcg ggatttcaca ccgagctgcc gctgatgacc agaatcttga tcggattttc
    12421 cgacttcatg gtgaattact ggatgctgat ctttgccttg gtagtcgggg cggtggtggc
    12481 gttccgctcc tatatcaaca cgacggcggg gcgttacaga tgggatcgct acaaatttca
    12541 cctgccgatc gcgggcccga tcattttcaa gtccacgctg gcgcgattct cccgcagtct
    12601 ggcgctttcc ttcaagagcg gcatgccggt gctgcagggg ctggacgccg tcgcgctggt
    12661 ggtggacaac gccttcatgc gcagccgcat cgaggggatg cgtgcgggcg tggagcgcgg
    12721 cgagagcata ttgcgcaccg cgatggacgc tggcgtgttc aatccgatgg tgctgcagat
    12781 gatcgcggtg ggcgaggaga ccggcgatct ggatggtctg atgttcgagg tggccgacat
    12841 gttccagcgt gaggtcgaat acgacgtgaa aaatctgagc tcgcagatcg agccgatcct
    12901 gatcgtcttt ctgggcgtgc tggtgctgat tctggcgctc ggtgtgtttt tgccgatgtg
    12961 ggatctgggc aaggcggcaa tgcacaaatg agtcaatccc cccgtcagca agctgttctc
    13021 cctctttggc aaaggggtgc ggggggaatt tgccagcgcg gttttacgct gatcgagttg
    13081 atcgtcgtca tcgtcatcgt ggtggtcatg gctggggcat tcctcggccg cgtctattac
    13141 tatcaggagc gggccgagaa gaccgcgatg gaaggggtgg ccgcgtcgat acagagtgcg
    13201 ctgaccatgc aatacggaca tatcctgata cacggcaagc cctctgactt gcccgcgctg
    13261 gcgcagggca atccgatgaa ctggttgcag aagaagccgg gcaattatgc cggggagttt
    13321 tacgagccga cgccgcaaag ggtggcgaca ggcagctgga tttttgatct caagacgcat
    13381 gacctgatct acgtgctgaa caatgccaac tatttcaaac cgggcaagga cggtgagaag
    13441 tggatacgct tccatgtcgc catcaactac gatccgccac tgccgacagc gtcacagaat
    13501 gcgccgctcg aattgacggg cgccctgttc gagcctgtcg agctctattc ctggttctga
    13561 gtatagaatg ccagccttca tttgcatcat cgcatcatgt cgactccgat agtgttggaa
    13621 attattctgt ggcgttctct ggccttcttt ctgctggttg gtgcggcggt ggggatcttc
    13681 gttgcgctgc tgctgatttt tcggtccgag cttctggggc gcatcaatcg ggtggcaaac
    13741 cgctgggttt cgacgcgcca tctggatcag gcgctggaac gcagtgtcag cgtcgaacat
    13801 tggtgctatc ggcatcacca gttgctgggg gcgctggtct gtctcggtgc cgtttatatc
    13861 tttgcgtatt ttggcctgcg gttcgacaag gcgttcgtca tggcgcatct ggcgggcctg
    13921 ttgcccggcg tgctgcttga cgtgctgctg gatgcgttgg tgctcaccgc gctgacgggg
    13981 gcggttgcgt ccctgtatgt cgggctgttg ctgtggttgc gtcccagcct gttgcgcggc
    14041 accgaggaag tggccaacca atggctgtct acgcgccggg ccgtccgccc gctggagatt
    14101 ccgcgcaatc agatggatga gttcgtggcg cgacacgcac agcgagtcgg ctggtggctg
    14161 ctgctgggca gtcttgtttt gctcgtcatg gtgctccgcg cgctggtgta gcaaagtcga
    14221 tacgggatgg cttgcgattg tacgccggcg ggtattgctc tatagttatc gaagtctggt
    14281 caatcttgct gcggcaaggg gtgtcaggcg gcatgaaata aaatcatcgg gctgcggctc
    14341 tgaactacag gagaattaaa atgcaaaaac aatctggttt taccctgatc gagctgatca
    14401 tggtgatcgt gattctgggt attctggcag cgaccgcgtt gccgaagttc gtgaatcttt
    14461 ctgctgatgc caaagatgca gcgctgaagg gggttgcagg cgggctggca tcggccaacg
    14521 tgatcaacgt cggcggctgt gccattgcgg gttcggccgt ggctggcaag tgcgtaaagg
    14581 tggccaagtg ttctgacgtg ggtacgctta tggagccgac tttgacactt ggaacgacca
    14641 caagcactac ggttccttat ctggcggctg atacagctgt tactgcagga agttcagtga
    14701 gctgtacagt tcagcaggat gctgctagtg ctgtgtacgc ggcaaccggg acgtaataac
    14761 gcgcaggatg ggttgagctg gtggggtact cgttaccgcc aacaaaaaag gaggcttcgg
    14821 cctccttttt tgttggcggt tcgcgcattt gttacactgc gggctcgtcc attgcgagcg
    14881 aaccctatcc gccgatgctt cacccatcca gtcaaatcaa agagcatgcg ccagagtcat
    14941 cccggcaagg gggcttcacg ctgatcgagc tgatcatggt gctggtcatc gccggcatcc
    15001 tcgcggtgtt cgtcgcgccg cgcttcttcg atgcgaatat tttcaaggcg cgcggttttg
    15061 ccgatcaggc gcaggcggcc atgcgctatg cgcagaagat cgccatcgcg cagcgccgtc
    15121 tggtttgtgt ggccgtgacg gcgccgacgg caacctccgc gagactggat ctggccatcg
    15181 ccgtttcgaa tgcggcgacc tcctgtgcga ctccgctggc attgcccgcg agcagtagca
    15241 acttcattca gtccagcgat gccacgacat cggccgcgaa cttcgtcttc gatgcgctgg
    15301 ggcgcccctt cgcgctgccc tatgcctcgg gcaatgcgag tgttgggcag acggtgacgg
    15361 ttgcgggcta tgccatcaag gtcgagaaag agacgggcta tgtccatcaa taactggagc
    15421 gccctacctt catttttccc cctttgcaaa aggggggctg gggattcgca gcgcggcatc
    15481 agcctgatcg agttgattat gttcatcgtg atcgtcagcg tcgcgctggc gggcatcatg
    15541 ctggtgatga acgtgacgac caggagcagt gccgatccgc tggtgcacaa gcaggcgctg
    15601 gcgatcgccg agtcgctgct ggaagaggtg gagctgatgc cgttcacgat ctgcgatccg
    15661 aacgatatca atgcggccag cgccgtaacc tctgccgatt gcaccggagg ggcagggggc
    15721 gcttatgacg aggccaggtt gccgctcggc ccggaaacga tttcgggggt ggtcgagact
    15781 cgccactcga ccacggaccc gttcgacaat gtcagcgact ataacggctt tgacagtgtg
    15841 gccgatggcg gcatcaagga tatcgccggt aatgcggtgg cgggattggg tgcttataag
    15901 gcgacggtat ccgtcgcgca gcaggtgata ggcggcatcc ctctgccaga atcctcgttg
    15961 atcaccgtga gcgtcaccgg ggcggatggg caggcggtgg tggtcgaagg cgtgcgggcc
    16021 cgctatgcgc cgagggcatt gccatgaagc ggatagttga aagtggatcg gcgctacgcg
    16081 cctcagtgga gcggcgctgc gcgcctcagt ggaaagaagc gcctttcggt cactcgctac
    16141 gctcttccca atttccactc accacttacc actcgccact tacctcgaag ggttttacgc
    16201 tggtcgaaat gatcatggtg attgtcatca ccggcatcat cggcggcatc gttgcggtgt
    16261 ttctcaaggc gccgatccag caatatgccg atgtggcgcg ccgtgcagat atgactgaca
    16321 tcgccgacac cgcgttgcgc cgcatgagcc gcgacttgcg cctggccttg cccaatagcg
    16381 tgcgcgtgag cggggcttgc gatggcgtag gcagttgcaa tcttgaattt atcccgaccc
    16441 atggtggcgg gcgctaccgc gcaggggtgg gtggcggtgc gggctgtgcg gcttctccgg
    16501 gcggagagga gctggatttc acggtggccg atacctgctt cggcgtgttg ggaaccatgc
    16561 ctgccatgtc ggcgggggat gagatcgtgg tgtacaacct aggcatcgcg ggagccgatg
    16621 cctacgcggg aaataccctg gcaagccaca atcggcgcgc cgccagtgcg gcgggaaata
    16681 tcgtcacgct gacttcggtc aatgcgctgc ccttcgattc tcccgggcac cgcttccacg
    16741 tggtgacgac gccagtgacc tatgtctgca cgcccaatgc actgaatccg gcgctgggaa
    16801 cgctaacccg tctttcgggc tacggctggg ctgcgcctgc gggcgcgggc gatcttctgg
    16861 ccaagggggt tggccgctgc ctcttttcct atgatccgct ggtggtcggg cgttcgggcg
    16921 tggtaaccat gcagcttgcc atcaccgagc agggagagac ggtctcgctg ttcagcgcat
    16981 ctcatgtgag taaccagcca tgatgaagag caggatcgag gatcgaggat tgagggttgg
    17041 tggtgcgcga ggcatcgcgg gtttaacttg cgcctcgctc tccgcgcctc gcttatcaaa
    17101 aggttttagt ctggtctctg ccatctttct gctggtggtg atcgctgcgc tgggcgcttt
    17161 tgccgtcacc ttgtccacca cgcagcagca gagtgccgcg ctggatgtgc tgggcgcgcg
    17221 cggctatcag gcggcgcgtg ccggtgtgga gtggggggcg tatcaggtgc tgcgcaatcc
    17281 ggccgggatt gcgtgtccgg tgggcggtgc atcgaacccg gtggcgatgc cgggagcgcc
    17341 gagcacgctg gcgatctttg cggtggatgt cagttgctgg agctatgcgc cgatctccga
    17401 ggcggctggg acggtcacca tgtaccgtct ggtttctacc gcaacgcagg ggacgcccgc
    17461 gacgccgaat tatgtcgagc gccagatgag cgtgaccatc gcccaatagc ggcgtttttt
    17521 ccggcgggaa aattgaggtt gtgacggggc gcttgttttg ctagaatcgc gccctcaatt
    17581 ttttggtaga aggtagttcg tccatgacaa ccgtacgtgt taaagagaat gagccgtttg
    17641 aagttgcaat gcgtcgtttc aagcgctccg tggaaaagac cggtctgttg accgatctgc
    17701 gtgctcgcga gttttacgag aagccgactg cagagcgcaa gcgcaagctg gctgctgctg
    17761 ttaagcgtca ctacaagcgt ctgcgcagcc aaactctccc tccaaagatg tactaagtct
    17821 tgcccctcgc gggtatgact ggttccggca tgagtctcaa gcaacagatc accgatgaca
    17881 tgaaagccgc gatgcgcgcc aaggaagcgg cgcgtctcgg ggctatccgt cttttgctcg
    17941 cggcgatgaa gcagcgcgag gtcgacgaac gcatcgagct gtcggatgcg gatgtggtct
    18001 cgatcatcga gaagatgctg aagcagcggc gcgactccat cagtcagtac gaggcggctg
    18061 gtcgccagga tctggcggat gtcgagaagt tcgaggtcgg catcttgcag gcctatatgc
    18121 cgcagcagat ggatgaggct gagatcaatg ccgcgatcgc cgaagtggtc gcagcaaccg
    18181 gcgcagctgg cccgcaggat atgggcaagg tgatgggtgt gctcaagccg aagctggctg
    18241 ggcgtgccga tatgggccgt gtctcggcgc tgatcaaggc gcaactcgcc aagtagttcc
    18301 gctttgttgt tccggcgcag gccggaatct ggccgattca acgactggat gccggatcat
    18361 gtccggcatg acgaaaatcc tgagtatcga cttgatgtaa gggcggtgct ggcgtgattc
    18421 caaaaagttt cattcaggac ttgctcaatc gtctcgatat cgtggacgtg gtcgagcgtc
    18481 atgtgccgct taaaaaagcc ggtgcgaact acgtcgcctg ctgtccgttc cacaacgaaa
    18541 aatccccctc gtttactgtc agtcagtcca agcagtttta ccattgcttc ggctgcggtg
    18601 cgcatggcac ggccatcggc tttgtcatgg agcatgtcgg cctcggattc gtcgaggcgg
    18661 tcgaggagct ggcgcagaat gccggcatgg aggtgcctca cgaacgcgct gcagcaggtg
    18721 agacctatca aaaagtagcg cccgatctgt atgaggtgat gcaggtcgcc acgcgctatt
    18781 accgtgaaca gctcaagcaa tcgccgcgcg ccatcgacta cctcaaggga cgcgggcttt
    18841 ccggcgaagt cgccgcacgt ttcggcatcg gctatgcgcc ggagggctgg cagagtctcg
    18901 ccgccgcgtt ccccgattac cagagcaatg cgctgagcga gaccgggctg gtgatcgtta
    18961 acgaggaaga tcacaagcgt tacgaccgct tccgcgaccg cgtcatgttc cccatcacca
    19021 acgtgcgcgg gcaggtcatc ggtttcggcg ggcgcgtgct ggacaagggc gagcccaaat
    19081 atctcaattc gcccgagacg ccgctgttcg agaaggggcg cgaactgtac ggcctgttcc
    19141 aggcgcaaaa ggcggtgcgc gccgcgcagc gggtgctggt ggtcgagggt tacatggatg
    19201 tggtggcgct ggcgcagcac ggcgtggatt atgccgttgc cacgctgggt acggcgacca
    19261 cgccttacca tgtgcagaag ctgctgcggg tggtcgatca catcatcttc tgtttcgacg
    19321 gcgacaaggc cgggcagaag gcggcatggc gagcgctgga aaatgcgctg ccgcatctgc
    19381 aggatggcaa gcgcatcagt tatctgttcc tgccggtcga gcatgatccg gacagtttca
    19441 ttcgcgagtt cggacacgat gcgttcgagc agcgggtgca tgaggcgatg ccgctgtcct
    19501 cgtacctgct gcgcgagatc agcgccgagc tcgatctgcg cagccaggag gggcgcaatc
    19561 aattgttgca gcgcgccaag ccgttgctca ccgcgattgc ggctccggcg gcagccctgc
    19621 tgctgcgcaa ggaagtcgcc gccctcgcgg gggtgagtca ggcggagctg gaggcgttgt
    19681 acgagatccg cccggtggcg cgccccgcgc gtcctgcgcc gaagaaggcc gggcgcgccg
    19741 cgccttcggg gcgacggctg ctgttgcaat gcctgattgc ccggcctgcg ctgggggcga
    19801 agttgccggc cgattggcag ggcgatggtg ccgaggatga ggtcatccac gtgttgctgg
    19861 cggcgctgcg cgagacggat tacaccctca gcggcccggc gctgacgcaa tattttcagg
    19921 gcacgatgca tgagcgggcg atcgcggccg ccgaggcgga catgctgagc tggggcgatg
    19981 agttcgatgt ggaggccgag tttacggggc tgatgaacag tctcgacaag gataggcgcc
    20041 gtcagttgtt cgccggtttg caggccaagg tcgctcagag cgggctggcc gggttgagcg
    20101 acgcggagcg cgaatgttac cggcaggcag ggcgttgagc gaagtctcgg gaaagattga
    20161 gaaaaacagg tagcaatcgg gtataatccg ccgcttttct gcggtagcaa agcaggtatt
    20221 acgcaattag gaatctggat atggcaaagc ctagcgacaa gaaaaaactg gacaagaaat
    20281 cggcagaggt gctgctgcag ccggtcgtcg acccgcaaca ggatatcgag gcgcgtcgca
    20341 cgcgcctgaa aaccctgatc gtactgggca aggagcgcgg ctacctgacc tatgccgaga
    20401 tcaacgatca cctgccggat atgctcgagg tcgagcagat cgaaggcgtg gtcagcatga
    20461 tcaacgacat gggtattcag gtgtatgacg tagcgccgga tgccgaggcg ctgatcatga
    20521 ccgacgccgc acctgcggtg gccgacgaag atgcggctgc cgaagccgaa gccgcgctgt
    20581 ccagcgtcga ctccgaattc ggtcgcacca ccgaccccgt gcgtatgtat atgcgcgaga
    20641 tgggtacggt cgagctgctg acgcgcgaaa aagaaatcga gatcgccaag cgtatcgagg
    20701 aaggcctgaa gcacatgatt caggcgatct ccgcatgtcc tgcgacgatc gctgaaatcc
    20761 tggcgctggc gaccaagatc gaaaacgatc agatgcgcgt cgacgagttg atcgacggtt
    20821 tcgtcgatgt cgaggcggcc gaggcagaag ccgccgccga agaagaggcg agcgaagaag
    20881 agagcgaaaa cgaagaggct gacgacgtcg acgaagagga agacgaggct gcaaccgctg
    20941 cggctgcagc cgccaatctg gcgcagctga aggccgaagc catgacgcgt tttgcggtga
    21001 tcgccgacca gttcaccaag atgggcaagg cctacgagaa gttcggctat cgcagcaagc
    21061 agtacgacaa gtgccagagc acgatctccg acgagctgat gaatttccgt ttcactgcca
    21121 agcaggtcga tgcgctgtgc gaaaccatgc gcagtctggt cgaggaagtg cgccgcagcg
    21181 agcgcagcat tcaggacatg tgcgtcacca agagcaagat gccgcgtccg cacttcatca
    21241 agaccttccc gggcaatgaa gagaatctcg aatgggttgc acaggagctg aaggccaaga
    21301 agccttacgc cgaagcgctt gcgcgttacc aacatgccat cgtcgagcag cagcagaatc
    21361 tgctgaacct ggaagcgcgt gtcggtattc cgatcaagga tttgaaagag atcaacaagc
    21421 agatgaccaa cggggaggcc cgtgcacgcc gcgccaagcg cgacatgatc gaggccaacc
    21481 tgcgtctggt gatctccatc gccaagaagt acaccaaccg cggtctgcag ttcctggatc
    21541 tgatccagga aggaaacatc ggtctgatga aggccgtgga caagttcgaa taccgtcgcg
    21601 gttacaagtt ctcgacctat gcgacctggt ggattcgtca ggccatcacg cgctccatcg
    21661 cggatcaggc gcgcaccatc cgtatcccgg tgcacatgat cgagaccatc aacaagatga
    21721 accgcatctc gcgccagatc atgcaggaga ccggcataga gccggacgtc gcgaccctgg
    21781 cgatcaagat ggaaatgccg gaagacaaga tccacaagat cctgaaaatc gccaaggagc
    21841 cgatctccat ggagaccccg gtgggcgacg acgacgactc tcatctgggc gacttcatcg
    21901 aagaccagaa caccacggtg cctgtcgatg cggccgtgta cgagagcctg cgcggtgcga
    21961 ccaaggatat tctggacagc ctgacgcaac gcgaagccaa agtgctgcgc atgcgtttcg
    22021 gcatcgagat gaacaccgat cacacgctgg aagaagtcgg caaacagttc gacgtcaccc
    22081 gcgagcgtat ccgtcagatc gaagccaagg ccctgcgcaa actgcgccac ccgtcgcgtt
    22141 cggaaaagct caagagcttc ctcgatagcg aaggataatc agtcacgggc cgttagctca
    22201 gttggttaga gcagaggact cataatcctt tggtccacgg ttcaagtccg tgacggccca
    22261 ccaatacagg ggattagcgt tgtgctaatc cccttttcat tatgagaaca gagccatgac
    22321 tgccgagttg tctgcaagtg cgctgcgccg caaggcgcgg ctggatgggc tgagcgcgat
    22381 cgagtttccc gccgacctgc cggtggtggc acggcgcgag gatctggcgc gcgcaattca
    22441 ggagcatcag gtggtgatcg tctgcggcga gaccggctcg ggcaagacca cgcagttgcc
    22501 gaagatatgc ctgacgctgg ggcgcggtgt gaaaggctgt atcggtcaca cccagccgcg
    22561 ccgcgtggcc gcacgcacgg tggcctcgcg catcgcgcac gagctcaaga ccgagctggg
    22621 cggcgcggtc ggtttcaagg tgcgcttcaa cgacaaggtg tcggcggaca ccagcatcaa
    22681 gctgatgact gacggcatcc tgctggccga gatccatcgc gatccgctgc tgcgcggcta
    22741 cgacaccctc atcgtcgacg aggcgcatga gcgctcgctc aacatcgact tcctgctcgg
    22801 ttatctgaaa cagttgttgc cgcgccgccg cgacctcaaa ctcatcatca cctcggcgac
    22861 gctggatgcg gaccggtttg cgaaacactt cgatgccaag gtggtggagg tgtcggggcg
    22921 cagctatccg gtcgaggtgc gttaccgtcc gccgcaggtg gacgaggaag gcaatacgca
    22981 ggacgtgccg caggcggtga gttccgcgct ggacgagctg gcggcaggcg ggctgcgcgg
    23041 cgacgtgctg gtgttcctgc cgggcgagcg cgagatacgc gacaccgcag aggtgctgcg
    23101 caagcatcat ccaaaaggca tcgaggtgct gccgctgttc gcaaggctgt cggcggcgga
    23161 gcaggacaag gtgttcaagc tgtcttccgg cgtgcgccgc gtggtgctgg cgaccaacgt
    23221 tgccgagacg tcgctgaccg tgcccaacat cggctatgtg atcgactgcg gcctcgcgcg
    23281 ggtgaaccgc tacagcatcc gccagaaagt cgagcagttg cacatcgaga aaatctcgcg
    23341 cgccgctgcc aaccagcgcg cgggacgttg cgggcgggtg atgagcggcg tgtgcatacg
    23401 tctgtacgac gaagagggct tcgcgcagcg ccccgagttc accgatcctg agatcttccg
    23461 cgtatcgctg gcgacggtca tcctgcggat gagcgcgctc gatctgggcg acatcgccga
    23521 gttcccgttc atcgagccgc ccagtccgcg catgatcgcc gacgggtatc agttgctggc
    23581 ggagctcggc gcgatcgacg agcagcggac gctgacgaag accggtcacg agctggccaa
    23641 gctgccgctc gaccccaaga tcgcgcggct gttgctggcg gggcggcaat accactgcct
    23701 gaacgagatt ctgatcatcg ccagcgcgct gtcggtgcag gatccgcgcg agcgtccgct
    23761 ggaccgtcag gaggcggcgg ataccgcgca caagcgtttt gccgacgagc gttccgactt
    23821 cctcgcttac cccaagctgt gggcctggta caggcaggcg gtggcggaca aaaaatccaa
    23881 tcgcctgatg gcggaggagt gtcgcaagaa tttcctgtcg ccgctgcgtc tgcgcgaatg
    23941 gcacgagctg catcagcaat tgcacacgca ggtcgccgaa atgggcatgc gcgaaaacga
    24001 gcagcccgcg acctacgaac agatccacaa ggcgctgctg tgcggcctgc tcggcaacat
    24061 cggcatgaag ggcgtggagg gcaacgaata tctgggcgcg cgcggcatca agtttctcgt
    24121 cgcctcgaac tcggtgctgg cgaagaaggg cgcgaagtgg gtgatggcgg cggagctgat
    24181 cgagacgcac cgactgtacg cacgctgcgt tgcacgcatc gagcccgagt ggctggaaga
    24241 gaccggcgac catttgatca agcgccatta ttacgatccg cactgggaga agaaggcggc
    24301 gcaagtggtc gcttacgaac gcagcacgct gcacggcttg ctgctgaacc cgaagaaacg
    24361 cgtgcattac gggccgatga atcccgccga gtcgcgcgag gtgttcatcc gccaggcgct
    24421 ggtcggggga gagttcaaca cgcaggcgcc gttcttcgct tacaaccaga agctgatcgc
    24481 cgacatcgag gcgctggagc acaaggcgcg ccgtcccgat gtgctggtgg acgacgaact
    24541 gatcttcgct ttctacgaca gccgcatccc ggcggatatc tacaacggtg cggctttcga
    24601 acactggcgc aaggaggcgg agcgcgacaa cgcgaagctg ctgtacctga aaaaagacga
    24661 tctgatgcgc cacgaggcgg ccggcatcac caccgaccag tttccgccgc aactcaagat
    24721 agacaatgtc agcttcgcgc tgagctataa cttttcgccc ggcaagaacg acgacggcat
    24781 cacgctgacg gtgccgctgg cgctgatcaa tcaggtggtg tcggcgcgct gcgagtggct
    24841 gatacccggc ctgctcgccg agaaggtgca gcagctgatc aagacgctgc aacagcggct
    24901 gcgccgccat ctggtgccgg tgccggaatt cgcggcggcc ttcgcccgcg atgtgccgcc
    24961 atcgaatacg ccgctgctgc aggcgctggc gcgttacatc cgcgaacaga aacagctcga
    25021 cgtgccgctg gatgccttcc gtctcgagca gctgccgcaa cacctgctga tgaatttccg
    25081 cgtgatagac gagcatggcc ggcagctcgg catgtcgcgc aacttcgcgc agctgcacgg
    25141 cgagctggca ccgcgcgtcg caccgatcgc cgtcgttgcg gctgcaacgg aaaagggcga
    25201 gtccacctcc gagcagcgct ataccgcgtg gagcttcggc gcgttcgagg agacgcgcac
    25261 cgtgcaacgc gccgggcaaa gcgtcacgct gttcaacgcg ctggtggatg aaggcgatgc
    25321 ggtggtgctg cgcgccttcg atacccgcga tgctgcaatg gccgcgcatc gcggcgggtt
    25381 gagtcgattg ttcatgctgc tgttgaaaga gcaggtgaag tatctggaga agaacctgcc
    25441 cgatgcgcag cgtctcggca tgctgttcat gcctttcggc acccagcagg agttgcagca
    25501 gcagatactc gcgacgactt ttgagcgctg ttgtctgggc gatccgctgc cgaccaacga
    25561 ggcggagttc aatgcgcgcg gcaaggaggc gaagaaccgc ctgctgctga tcgcacagga
    25621 gatcgcgcgt ctggttgcca ctatcctcac cgagcatcag gcgttgcaga agagcctgcc
    25681 gcagctcaag gctcatgccc aggtcgctca ggatgtgcgc gcgcagctgg aatggctgct
    25741 gcacaagagg tttgttcgtg acacccctta cgagcgtctg cagcatgtgc cgcgttatct
    25801 caaggcgatc aacctgcgca tcgagaagct gcgcaccaac ccggcccgcg acgcgcagtg
    25861 cgtctcgcag atacagccgc tgacacaggc cttgcagaag ctgcgtcagg cgcagcaggg
    25921 caagcccgat ccgcgtctgg aagagttcgc ctggctgatg caggagctgc gcgtgtcttt
    25981 gttcgcgcag gaattgaaga cgccggtgat cgtgtcggtg aaacgcctgc agaagatgct
    26041 ggagtcgttg cggagcaact aagtgtataa gcggtgaaat agaaaaagcc tcgtatcgct
    26101 acgaggcttt tttcgaatca atcaaatcaa cctgattgat cttgcgtgct accgcacgct
    26161 aaatttggtg gcccggggcg gaatcgaacc accgacacaa ggattttcaa tcctctgctc
    26221 taccgactga gctaccaggc catttgctgc attccgattc ttgctgctga accgagaagc
    26281 cgcgcattat acctataatg cggcaaataa caaaccccgc cgaagcgggg ttgttttagt
    26341 gcagtgaaac gaaccgcaat tacatcatgc cgcccatgcc acccatgccg cccatctcgc
    26401 cgcccatgcc gccagctggc ttgtcttccg gcgattcggc gaccatgcat gcggtcgtca
    26461 gcatcaggcc tgcgatggag gatgcgttct gcaacgcggt gcgagtcacc ttggttggat
    26521 cgaccacgcc catagccagc atgtcgccat actcgccggt gcctgcgttg taaccgaagc
    26581 tgcctttgcc ttccagcacc ttgttcacga ctacggatgg ctcttcgcct gcgttggcaa
    26641 cgatggcacg cagcggtgct tccattgcgc gcagcacgat ggtgatgcct gcgtcctggt
    26701 cgtggttgtc gcccttcagg ccggctaccg cagccttggc gcgcagcagt gccacgccgc
    26761 cgccaggtac gatgccttct tcaactgctg cacgggttgc atgcaatgcg tcttcgacgc
    26821 gggccttctt ctctttcatc tcgacttcgg gagccgcgcc gaccttgatc actgcaacgc
    26881 cgccggacag cttggctttg cgttcctgca acttctcttt gtcgtagtcg ctggaggact
    26941 cttccatctg cttgttgatg gtgacgatgc gggactggat cgcttcggtc ttgccagcgc
    27001 cgtcgatgat gatggtgttg tccttgccca cttcgatgcg cttggcttgg cccagatctt
    27061 ccagtgtggc tttttccagt gtcaggccga cttcttcggc gatcacggtg ccgccggtca
    27121 ggatggcgat gtcttccagc atggccttgc ggcggtcgcc gaagccgggt gccttgacgg
    27181 caacggtctt caggatgcca cggatgttgt tcaccaccag ggttgccagc gcttcgccat
    27241 cgacgtcttc cgcgatgatc agcagtggac ggctggactt ggcgatctgt tccaggatag
    27301 gcagcaggtc gcggatgttg gagatcttct tgtcgtgcag caggatgaac ggattgtcca
    27361 ttgcggcgat ctgacggtcc tgattgttga tgaagtacgg ggacaggtag ccacggtcga
    27421 actgcatgcc ttcaaccacg tccagctcat cttccagacc ggagccgtct tcgatggtaa
    27481 tgacgccttc cttgccgacc ttgtccatcg cttcagcgat cttgtcgccg actgcgctgt
    27541 cggagttggc ggagatggag ccaacctgag cgatttcctt gctggtggtg caaggcttgg
    27601 acagcttctt cagctcttca actgctgtca gcacagcctt gtcgatgccg cgcttcaggt
    27661 ccatcgggtt catgccggca gcgacgaact tcatgccttc ctgaacgatg gattgcgcca
    27721 gtacggtcgc ggtggtggtg ccgtcaccgg ccacgtcgga ggtcttggaa gccacttcct
    27781 tgaccatctg cgcgcccatg ttctcgaact tgtctttcag ttcgatttcc ttggcgacgg
    27841 aaacgccgtc cttggtgatg gtcggtgcgc cgtaagagcg gtccagcacg acgttacggc
    27901 ctttagggcc cagtgttacc ttgactgcgt cggccagaat gttcacgcca ataatcatct
    27961 tgctgcgtgc ggcgtcgtgg aattttacgt ctttagctgc catgtctatg tctcctgaaa
    28021 ttcgttggtt agttaggctt cgattacgcc gatgatgtcg tcttcgcgca tttgcagcag
    28081 ctcaacacca tccatcttga aggcctggcc ggaatatttt ccgaacaaca ccttgtcgcc
    28141 gaccttgacg ttcatcgggc ggaccttgcc gtcgtcaccc atcttgccgt tgcctaccgc
    28201 gacgatttcg ccctggtctg gcttctcggt ggctgcatcg ggaatcacga taccggatgc
    28261 ggtcttgcgc tcttcttcca tgcgcttaac gatgacgcga tcatgcaaag gacgaatttt
    28321 catgcttagt tctcctgaaa cggttaattg aatgcgaagg ccggttcggt gccagcggat
    28381 tagctttgca tagccatctg actacgctaa ctaagtcagc ggttagcact ctcggccagc
    28441 ga
//
LOCUS       DASUIV010000012         6620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_653406, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000012 DASUIV010000000
VERSION     DASUIV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653406"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFW59_00470"
     CDS             complement(<1..595)
                     /locus_tag="VFW59_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362719.1"
                     /translation="MKKLGVFFGKQWVRYFLLTITLFAVLETGIRVHETSVASLQDSA
                     LIKLQIFSLRSKTEILFLGSSRSQDGVNPTLVAETLSKLNPQWENTKAFNAAAPGSGL
                     RRLLYGAEMATKKPGIRLMGVEVSDPYLSDGAWTLPFGSDERATDLEGRLQQALSKYS
                     YAIRYRKSFRLDNAIKFPLLLWPDADDGIKWFGKVGAN"
     gene            complement(725..2305)
                     /gene="guaA"
                     /locus_tag="VFW59_00475"
     CDS             complement(725..2305)
                     /gene="guaA"
                     /locus_tag="VFW59_00475"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5362720.1"
                     /translation="MHKKILILDFGSQYTQLIARRVRETNVYSELHSWEMTEEEIRAF
                     DPAGIILSGGPNSVYEEETPKAPQVVFELGVPVLGICYGMQTMAAQLGGKVESGKVRE
                     FGYAEIRAQGHSKLFDGIQDKTNAQGHGLLDVWMSHGDKVTALPAGFSAIASNEATPF
                     AAMADEARRFYAVQFHPEVTHTHQGRAILGRFVHDICGCGQDWNMPDYITEAVEKIRA
                     QVGEEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDNGLLRLNEGEQVMETFGKNL
                     HMKVIHVDASAEFMKHLTGVSDPEQKRKIIGREFVEVFQRESAKLQQAKWLAQGTIYP
                     DVIESAGAKTKKAHTIKSHHNVGGLPASLHLKLLEPLRELFKDEVRELGVALGLPADM
                     VYRHPFPGPGLGVRILGEVKSEYAELLRRADAIFIEELRATRDASGKSWYELTSQAFT
                     VFLPVKSVGVMGDGRTYEWVVSLRAVQTQDFMTAHWAHLPYELLGRVSNRIINEVRGI
                     NRVVYDISGKPPATIEWE"
     gene            complement(2384..3844)
                     /gene="guaB"
                     /locus_tag="VFW59_00480"
     CDS             complement(2384..3844)
                     /gene="guaB"
                     /locus_tag="VFW59_00480"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5362721.1"
                     /translation="MRIIQKALTFDDVLLVPAYSNVLPRDVTLRTQLTRNISLNIPLL
                     SAAMDTVTESRLAIALAQEGGIGIVHKNMTDKAQAAEVAKVKRFESGVVKDPITITPD
                     MTVRNVIALTRQHRISGLPVVQGKQVVGIVTNRDLRFESNLDQSIQNIMTPRERLITV
                     KENASLDEARNLMHKHRIERVLVVNDAFELRGLMTVKDILKSTEHPRASKDSQGRLRV
                     GAAVGVGAGTEERVTLLAEAGVDVIVVDTAHGHSQGVLDRVKWVKDNFPQVEVIGGNI
                     ATGAAALALLDHGADGVKVGIGPGSICTTRIVAGVGVPQIAAIQNVAKALQGTGVPLI
                     ADGGIRYSGDISKAIAAGAHAVMLGGLFAGTEEAPGEIELFQGRSYKSYRGMGSLGAM
                     QQGSSDRYFQEGESNQDKLVPEGVEGRVPYKGSVLAVIHQLMGGLRASMGYLGCADIA
                     TMHVKAEFVEISSAGIRESHVHDVQITKEAPNYHID"
     gene            complement(3931..4269)
                     /locus_tag="VFW59_00485"
     CDS             complement(3931..4269)
                     /locus_tag="VFW59_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5362722.1"
                     /translation="MKKIEAIIKPFKLDEVREALSELGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKVEVVVATSLLETAVEAIVKAAHTGKIGDGKIFVSTVERVIRI
                     RTGETDESAL"
     gene            complement(4529..5086)
                     /gene="greB"
                     /locus_tag="VFW59_00490"
     CDS             complement(4529..5086)
                     /gene="greB"
                     /locus_tag="VFW59_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX5362723.1"
                     /translation="MSKAFTKETDGDDEDELEAASPLPAGVKNYLTPGGYRKLRDELD
                     HLWKVERPALVKTITWAASNGDRSENGDYIYGKKRLREIDRRVRFLRKRLDQAEVVDP
                     STRGECDQVFFGATVTVCDEQGCENIYSIVGVDEADVGNGRISWVSPLARALTKLREG
                     EVAILRTPVGITELDIVSVVYCSLD"
     gene            complement(5151..>6620)
                     /locus_tag="VFW59_00495"
     CDS             complement(5151..>6620)
                     /locus_tag="VFW59_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX5362724.1"
                     /translation="TKPEDIHGFFASRGILTSRGGKTSHAAVVARGMGKPCVAGAEGI
                     EVNVLLRQATIGETVIHEGDIITIDGTTGLVYLGTVPTIEAEITDELNVLLGWADQEA
                     RLAVMANADTPDDAKRALQFGAKGIGLCRTERMFNASDRLPIVLDMIIAEDTLQRQAA
                     LDRLLPIQRGDFKGLLMTMSGLPVTIRLLDPPIHEFLPSEQQLIKELAELRHLHQTAS
                     GMEILSNTLDLLGDAELSSSDVAHVRLNAVNPHIVEQAISKKQAILKKVRTLHEVNPM
                     LGHRGVRLSVTYPEIYAMQIRALAEATVECIKEGADVHPEIMVPQVCSAAELRYVRKH
                     VDRIIAEVEQVSGIKVKIKFGSMMEVVRACMRADTLAGEAEFFSFGTNDLTQATYSFS
                     REDAENKFLPMYIEQGILRDNPFEVLDVEGVGRLMKLAIEGGRSVKPALKIGICGEHG
                     GHPASINFCHQIGLNYVSCSAPRVPIARLAAAHAKLNEV"
BASE COUNT         1339 a         2080 c         1850 g         1351 t
ORIGIN      
        1 aattggcccc caccttgcca aaccatttga tgccatcatc agcatcgggc caaagcagca
       61 gggggaattt gatagcgttg tcgagccgaa agcttttccg gtaacggatc gcataagaat
      121 attttgaaag cgcttgctga agccgccctt caaggtcagt tgcgcgctca tcggaaccga
      181 aagggagagt ccaggccccg tcggacagat aaggatctga aacctcgacc cccatcagcc
      241 gaatgcccgg cttcttagtg gccatctcgg caccgtagag caaccgtctc agcccggagc
      301 cgggcgcggc ggcattgaag gccttcgtgt tttcccattg aggattcagc ttgctcaagg
      361 tctcggccac aagggtggga ttgaccccat cctggctgcg cgatgagcct aaaaacagaa
      421 tctcggtctt gctcctcaat gaaaagatct gcaacttgat gagcgcgcta tcctgcaacg
      481 atgccaccga agtctcgtga acacgaatcc cggtttccag aacagcgaac aacgttattg
      541 tcagcagaaa gtaacgtacc cactgcttac caaaaaaaac gccaagtttc ttcatgacat
      601 catctcaaag gccgagccct gacaccgaag ttaagcttct cggtcttgcc caagccccgc
      661 ccacgaagcc gacagaccga tttcacgaga taacgagatc atcccgctta accagccaga
      721 ctcatcattc ccactcgatg gtggcaggcg gcttgccgga gatgtcgtag accacgcggt
      781 tgatgccgcg cacttcgttg atgattcggt tggacacccg gcccagcaac tcgtaaggca
      841 gatgcgccca gtgtgcggtc atgaagtcct gcgtttgaac agcacgcaag gacaccaccc
      901 attcgtaggt gcggccatca cccatcacgc cgacactctt gaccggcagg aacaccgtga
      961 aggcctgcga ggtcagttca taccaggact tgccgctggc atcgcgggtc gcgcgcaatt
     1021 cttcgatgaa gatcgcatct gcgcggcgca gcaactcggc atattcgctc ttcacttcgc
     1081 cgagaatgcg cacgcccaga ccggggccgg ggaaaggatg acgatacacc atgtcggccg
     1141 gcaggcccaa ggcgacgccc agttcgcgca cttcgtcctt gaacaattcg cgcaacggct
     1201 ccagcagttt cagatgcagg gatgccggca ggccgccgac gttgtggtgc gacttgatgg
     1261 tgtgggcctt cttggtcttg gcaccggccg actcgatcac gtcggggtag atggtgccct
     1321 gtgccagcca cttggcctgt tgcagcttgg ccgattcgcg ctggaacact tcgacgaatt
     1381 cgcgcccgat gatcttgcgc ttctgctcgg ggtcggaaac gcccgtcagg tgtttcatga
     1441 actcggcgct ggcatccaca tgaatgactt tcatgtgcaa attcttgccg aaggtctcca
     1501 tcacctgctc gccctcgttc aggcgcagca ggccgttgtc cacgaacacg caggtgagct
     1561 ggtcgccgat ggcgcgatgg atcagcgccg ccgcgaccga ggaatccacg ccgccggaca
     1621 ggccgagaat gacttcttcc tcacccacct gagcgcgaat cttctccacc gcctcggtga
     1681 tgtaatccgg catgttccag tcctggccgc aaccgcagat atcgtgcacg aagcggccga
     1741 ggatggcacg gccctgatgc gtgtgggtga cttcaggatg gaactgcacc gcatagaagc
     1801 ggcgcgcctc gtccgccatc gcggcgaaag gcgtggcctc attggaggca atcgccgaga
     1861 aaccggcagg caatgcggtg accttgtcgc cgtggctcat ccacacatcg agcagaccgt
     1921 ggccctgcgc attggtcttg tcctgaatgc cgtcgaacag cttggaatgc ccctgcgcgc
     1981 gaatctcggc ataaccgaac tcgcgtacct tgccactttc gaccttgccg cccaactgcg
     2041 ccgccatggt ctgcatgccg tagcagatgc ccagcaccgg cacacccagt tcgaacacga
     2101 cctgtggcgc cttcggcgtc tcttcctcgt acaccgagtt agggccgccg gacaggataa
     2161 taccggccgg atcaaaggcg cgtatctcct cctctgtcat ctcccatgag tgcaactcgg
     2221 agtagacatt cgtctcgcgc acgcgacgcg cgatgagctg ggtgtattgg gaaccgaaat
     2281 cgagaatgag gattttttta tgcatggctt tttcaggatt gaggattcag gaatgaggat
     2341 ttaggcgaga ttttgctcaa tcctaaatcc tcgcgcctca atcctagtcg atatggtagt
     2401 taggtgcttc tttggtgatc tgcacgtcat ggacatgaga ctcgcggata ccagcagaag
     2461 atatctcgac gaactcggcc ttgacatgca tggtggcgat atcggcacag ccgaggtagc
     2521 ccatgctggc acgcaggccg cccatcagct gatggatcac ggccagcacg ctgcccttgt
     2581 acggcacacg accttcgacg ccttccggca ccagcttgtc ctgattgctt tcgccttcct
     2641 ggaagtagcg gtcgctggag ccctgctgca tcgcacccag actgcccatg ccgcgataag
     2701 acttgtagga gcgtccctgg aacaattcga tctcgcccgg tgcctcttcg gtgccggcga
     2761 acaaaccgcc caacatcacc gcatgcgcgc ctgcggcgat tgccttggag atgtcgccgg
     2821 agtaacggat accgccgtcg gcgatcagcg gaacgcccgt gccctgcagt gctttggcca
     2881 cattctggat ggccgcgatc tgcggcacac cgacgcccgc aacgatgcgc gtggtacaga
     2941 tcgagcccgg gccgataccg accttgacgc cgtccgcacc gtgatccagc aaggccagtg
     3001 ctgcagcccc cgtggcgata ttgccgccga tcacctccac ctgcgggaaa ttgtctttga
     3061 cccacttgac gcgatccagc acgccctgag aatgaccatg tgcggtatcg accacgatca
     3121 catccacgcc ggcttcggcc aacagcgtga cgcgctcttc ggtacccgca ccgacgccga
     3181 ctgccgcgcc gacgcgcaga cgtccctggc tatccttgct cgcgcgcgga tgctcggtag
     3241 acttcaggat atctttcacc gtcatcaggc cgcgcaactc gaatgcatcg ttgaccacca
     3301 gcacgcgttc gatgcgatgc ttgtgcatca gattgcgcgc ctcgtccagg ctggcgttct
     3361 ccttgacggt gatcagacgc tcgcgcggcg tcatgatgtt ctgtatggac tgatccagat
     3421 tgctctcgaa acgcaggtcg cggttggtca cgataccgac cacctgcttg ccctgcacca
     3481 ccggcagacc ggaaatcctg tgctggcgcg tcagtgcgat cacattgcgc acggtcatat
     3541 ccggcgtgat ggtgatcgga tccttgacca cgccgctttc gaaacgcttg accttggcga
     3601 cctcggcggc ctgcgccttg tcggtcatgt tcttgtgcac gatgccgata ccgccctcct
     3661 gcgccagcgc aatcgccaga cgggattcgg tcacggtgtc catcgcggcg gacagcagcg
     3721 ggatgttcag gctgatgttg cgggtcaact gggtgcgcaa agtgacgtcg cgcggcaaca
     3781 cattggagta ggctgggacg agcagaacgt cgtcaaaggt cagtgctttt tgaatgatgc
     3841 gcatgtgaaa cggccctcgg caaagcgagc attatacgca tgcagggcgg tcgggtaaac
     3901 gacccagcct gcatgtccag aaaatacagc ttacagcgcg ctttcgtctg tctcgccggt
     3961 acggatgcga atgacccgct ccaccgtcga aacaaatatc ttgccgtcgc cgatcttgcc
     4021 ggtatgggct gccttgacga tcgcttcgac ggccgtctcc agcagactgg ttgccaccac
     4081 cacctcgacc ttgatcttag gcagaaaatc gaccacatat tcagcgccgc gatacagttc
     4141 cgtatggccc ttctgacggc caaaaccctt gacctcggtg actgtcaggc cgctcacacc
     4201 gagttcggac agcgcctcgc gcacttcgtc cagtttgaac ggtttgataa tcgcttcgat
     4261 tttcttcatg gagacttccc tctatgcatt ggttgactgg ttattccgct tgaaccggcg
     4321 gcattgtatc aattaagaat catgatgcct cgaaaaaccg tcgcgagcgg ctcgagtgca
     4381 aggcgcacgg tgctttgcat ggccaatcga ctaacctggc agaaaacgcc agccaagtcg
     4441 ctggtcaacg cagaaacctg aatgtgctta cagtacatga ggatttcgag taccgcgcaa
     4501 cgccgcaatc gagtcgcgca gtaggttttc aatccagaga gcaatagacc acactcacga
     4561 tatccagctc cgtgataccg accggcgtgc gcaatatcgc cacctcgccc tcgcgcaact
     4621 tggtcagcgc gcgcgccagc ggcgaaaccc agctgatccg cccattgccg acatcggcct
     4681 cgtccacgcc gacgatgctg tatatgtttt cgcaaccctg ttcatcgcac acggtaacgg
     4741 tcgcaccgaa gaatacctga tcgcattcgc cgcgcgtcga tggatcgacc acctcggcct
     4801 gatccagccg cttgcgcagg aatcgcacac ggcggtcgat ctcgcgcaga cgcttcttgc
     4861 cgtagatgta gtcgccattc tcagaacgat caccattgga ggcagcccag gtgatggttt
     4921 tcaccaatgc cggacgctcg accttccaga gatgatccag ttcatcgcgc aacttgcgat
     4981 agccacccgg tgtgaggtaa ttcttgacgc ccgcaggcaa cggactcgcc gcttccagct
     5041 cgtcttcgtc gtcaccatcg gtttcttttg tgaatgcctt gctcatacgg tgaattctac
     5101 agcaagcccg gcggtttccc gccggtcagg gagaggatgc agccgactac tcaaacttcg
     5161 ttgagttttg catgcgcagc cgccagccgg gcgatcggca cgcgcggcgc cgaacaggac
     5221 acataattca acccgatctg gtgacagaag ttgatcgagg ccggatgtcc gccatgctca
     5281 ccgcagatgc cgatcttgag cgccggtttg accgagcgcc cgccctcgat ggccagtttc
     5341 atcaatcggc cgaccccctc tacatccagc acctcgaacg ggttgtcgcg caggatgccc
     5401 tgctcgatgt acatcggcag gaacttgttc tcggcatcct cgcgcgagaa cgaataggtt
     5461 gcctgagtca ggtcgttggt gccaaacgag aagaactccg cctcccctgc cagcgtgtcg
     5521 gcgcgcatgc aggcgcgcac cacctccatc atcgagccga acttgatctt caccttgatg
     5581 ccgctgacct gctcaacctc ggcgatgatg cgatcgacat gttttcggac gtagcgcaat
     5641 tcggcggcgc tgcacacctg cggcaccatg atctcgggat gcacatccgc gccttctttg
     5701 atgcactcga cggtggcctc ggccagcgcg cgtatctgca tcgcgtagat ttcgggatag
     5761 gtgacgctca gacgcacgcc gcgatggccc agcatcggat tgacctcgtg cagcgtgcgc
     5821 acctttttca ggatcgcctg cttcttgctg atcgcctgct cgacgatatg aggattgact
     5881 gcattcagcc ggacatgcgc gacatcggaa gaactcagct ctgcatcgcc cagcagatcg
     5941 agcgtgttgg acaggatttc catgccgctg gcggtctggt gcaaatggcg caactcggca
     6001 agttccttga tcaactgctg ttcggagggc agaaattcgt ggataggcgg atcgagcagg
     6061 cggatcgtca ccggcaggcc gctcatcgtc atcagcaaac ccttgaagtc gccgcgctgg
     6121 atcggcaaca gacggtcgag cgcagcctga cgctgcagcg tatcctcagc gatgatcata
     6181 tccagcacga tgggcaaacg atcgctggca ttgaacatgc gttcggtccg gcacagaccg
     6241 atacccttgg caccgaactg caaggcgcgt ttggcatcat ccggggtatc ggcattggcc
     6301 atcaccgcca gtctggcttc ctgatccgcc cagcccagca gcacgttcaa ctcatcggtg
     6361 atctcggcct cgatggtcgg cacggtgccc agatagacca gaccagtcgt gccgtcgatg
     6421 gtgatgatgt cgccctcgtg gatcaccgtc tcgcctatgg tcgcctgacg caagaggacg
     6481 ttgacctcga tgccctcggc accggccacg cagggcttgc ccatgccgcg cgcgaccacg
     6541 gccgcatgcg aggtcttgcc gccgcgactg gtcagaatgc cgcgcgaagc gaagaaacca
     6601 tgaatgtctt ccggtttggt
//
LOCUS       DASUIV010000013        11509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_653751, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000013 DASUIV010000000
VERSION     DASUIV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 11509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11509
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653751"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..505
                     /locus_tag="VFW59_00500"
     CDS             2..505
                     /locus_tag="VFW59_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX5362725.1"
                     /translation="MMHLLLASALLTGCAGEEFQDLRDFVQNAGADLRGKIPPPPEVK
                     PYEPFVYDNVDNLQDPFKPRKPDSRSASRPGLNQPDMDRPKEALEEFPLEGLHMVGYL
                     RQNKVGYAIIRAPDGKLHRVRAGNYVGMNFGLIKEVGETEIVVQETVQDSAGDWSERI
                     SSLQLVE"
     gene            599..2656
                     /gene="pilQ"
                     /locus_tag="VFW59_00505"
     CDS             599..2656
                     /gene="pilQ"
                     /locus_tag="VFW59_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX5362726.1"
                     /translation="MKPFNSVLRGMAQVLGLFVALGMSAAQAEVQNSITALGVSSAGN
                     GATVVKVEFAQPLTSLPHDFTINTPPRIALDFSNTANGLGKSTQEFTEGELRSANIVQ
                     AGTRTRLVINLNQMLTYSTKIDGNNLLVTLQGKVAANADTGSTSRFAEVKQSAQKHSL
                     RDIDFRRGKNGEGRIQVDLSDAGVGIDIRQQGTTLVVDFLKSSLPKNLQRKLDVVDFA
                     TPVQGVDTFVQGDNVRMVIEPKGLWEHAAYQTDNKFIVEVKPIADDPNKLVKGSQAGY
                     AGEKLTLNFQKIDVREALNVIADFTELNMVISDTVSGNLTLRLKDVPWDQALDIILQS
                     RGLDMRKNGNVIQVAPRDEIAAKEKIDLTSRQEISDLEELRTESFQLSYQKGDAVTRL
                     LSGDKQRILSKRGSTVVDPRTNTLFVQDTPSHLDEVRKLIKQIDVPVRQVMIEARFVS
                     ATDDFNRTLGGKLGYTGPAVGVGNGFGIGGRPNSTQGVIGGNVNLPGAASGTGGLTLS
                     LFNPAATKVLTLELTASELDGKTKNIASPRVVTADKTQAKISAGTQIPYQQASSSGAT
                     AVAFKDATLSLTVTPQISPDDNVDMKLDVNQDTVGAIYGGVPSINTKQVSTQVMVENG
                     GTVVIGGVFTQNIGDSTQKVPVLGDVPVLGWLFKNNVKSDTKSELLIFITPKIVKDAM
                     NLH"
     gene            2728..3357
                     /locus_tag="VFW59_00510"
     CDS             2728..3357
                     /locus_tag="VFW59_00510"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX5362727.1"
                     /translation="MHSDHANSRPKGGRKFNSGNLILVGLMGSGKTTMGRALAKHLGK
                     TFVDSDDEVQQRTGVTIPHIFDIEGESGFRQRETAAIRELVERDDMVLATGGGAVLAE
                     ENRVLLQQNGIIVYLKANVHDLWLRTRHDRSRPLLQTGDPRAKLTELFQQRDPLYQQV
                     ADITVQSGKQTVHGLMLYLVNEIEAFKANDASQGRVISGHGIEHEQSKI"
     gene            3368..4453
                     /gene="aroB"
                     /locus_tag="VFW59_00515"
     CDS             3368..4453
                     /gene="aroB"
                     /locus_tag="VFW59_00515"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX5362728.1"
                     /translation="MQTLTVGLGERSYPIHIGSGLLAQPALLQAHLPRKRAAIVTNTT
                     VAPLYLDKLQQTLQAIGVNSISVVLPDGEEHKNAETLNLIYDALLANRCERSTPLIAL
                     GGGVIGDMTGYAAASYLRGVPFIQIPTTLLSQVDSSVGGKTGINHPLGKNMIGAFYQP
                     QLVLADTDTLNTLPDNELAAGLAEVIKYGLIRDLPFLEWLEQNMDKLLARDTTALQYA
                     IARSCQNKAEVVAADERESGERALLNLGHTFGHAIESGMGYGNWLHGEGVAAGTVMAA
                     DLSQRLGWIDARDVARIRKLFERARLPVISPDLGTEKYLDLMGLDKKVEGGKVRFVLL
                     RELGRAVIDGDVPPALLQQTLEACVRG"
     gene            4446..5576
                     /locus_tag="VFW59_00520"
     CDS             4446..5576
                     /locus_tag="VFW59_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX5362729.1"
                     /translation="MADFAPYSVSEANSRGRKIAEAAPAGRGEFQRDRDRIIHSSAFR
                     RLEYKTQVFVNHEGDLFRTRLTHSIEVAQIARSIARRLHLNEDLAEAISLAHDLGHTP
                     FGHAGQDALNACMQAYGGFEHNLQSLRVVDLLEERYAEFEGLNLTFETREGILKHCSP
                     DNAARLGELGERFLNDRRPSLEGQIANLADEIAYNNHDVDDGLRSGLITLEQLAEVPL
                     VARHLDEVRAAYPALPERRVVHETVRRMINTLVSDLIRQSESNIAAQKLETLEDVHRA
                     PVLIAFSDEMNAQQRVLKGFLRVNLYRHYRVMRMSAKAQRIISDLFGVFMGDPRLLPP
                     QFQQREGNGEAERARLVADYIAGMTDRYAIREHRRIFAVEEA"
     gene            5738..10381
                     /locus_tag="VFW59_00525"
     CDS             5738..10381
                     /locus_tag="VFW59_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX5362730.1"
                     /translation="MQQGLYDPRQERDACGVGFVAHIKGKKSHDMVRQGLQILENLTH
                     RGAVGADPLAGDGAGILIQIPDQFLREEMGWGNIQLPAVGSYGVGIMFLPRDAAARAA
                     CEQIVADKIAAEGQSVLGWRDVPVDGSGLGESVKKVEPVVRQVFIARGENCADVDAFE
                     RKLFVIRKTVEHAVRGLGSNQGTGFYIPSLSARTIVYKGMLLADQVGKYYLDLKDERV
                     VSALALVHQRFSTNTFPTWDLAHPFRMIAHNGEINTVRGNVNWMTARHAAMSSQLLGE
                     DLEKLWPLIVDGQSDSACFDNALELLVAGGYSLPHAMMLLIPEAWAGNPLMDEERRAF
                     YEYHAALMEPWDGPAAVAFTDGRMIGATLDRNGLRPARYLITDDDMVLMASEMGVLDI
                     PQHKIVKKWRLQPGKMFLIDMEAGRIVDDVELKTQLSTAKPYRQWIEKSRYFLGDLPK
                     AKGDVKLNASLLDTQQAFGYSQEDLKFILAPMVNAGEEPTGSMGIDAALPVLSNKNRS
                     FYDYFQQLFAQVTNPPIDPIREEIVMSLTSFIGPKPNLLGIDETNPPLRLEVHQPVLS
                     NDDMVKLRNIDKLTKGEYKSQVLDITYPAKNGAEGCEAAIRALCAAADKAVAGGYNVL
                     ILSDREMSEKRVAIPALVACAKVHHHLVSEGLRTSAGLVVDTGSAREVHHFALLAGYG
                     AEAVCPWLVYETIADMKLPANVDAKEAGKRFIKAINKGLMKVMSKMGISTYQSYCGAQ
                     IFEAIGLNASFVAQYFTGTATQIEGIGLKEVAEEAVRTHRAAFGADPVLATALDAGGE
                     YAYRVRGEEHMWTPDSIAKLQSATRTNNAATYKEYAKLINEQSRKLKTLRGMFEIKPV
                     GAPVPLNEVEPAKEIVKRFVTGAMSLGSISTEAHTTLAIAMNRIGGKSNTGEGGEDAA
                     RFRTLKAGEKLSDIIGKNRIEKDFVMKEGDSLRSRIKQVASGRFGVTAEYLSSADQIQ
                     IKMAQGAKPGEGGQLPGGKVSEYIAKLRFSVPGVGLISPPPHHDIYSIEDLAQLIHDL
                     KNSNPTASISVKLVSEVGVGTVAAGVSKAKADHIVVSGFDGGTGASPLSSIKHAGTPW
                     ELGLAEAQQTLVLNQLRGRIALQVDGQLKTGRDVLIGALLGADEFGFATAPLVVEGCI
                     MMRKCHLNTCPVGVATQDPELRKKFTGQPEHVVNYFFFVAEEARELMAQMGIRKFDDL
                     IGRSDLLGVKEGIAHWKSQGLDFSRVFHQPDMPASVARRHAEAQDHELNKALDNTLIA
                     KAQGALKDKKPVVIESRVGNVNRTVGTMLSHEVAKRYGQAGLPSDTIQVKFTGTAGQS
                     FGAFLAHGISFELRGEGNDYVGKGLCGGRIAIRPPAEANLVAHENTIVGNTVLYGATS
                     GECYFNGVAGERFAVRNSGAIAVVEGVGDHGCEYMTGGMVIVLGQTGRNFAAGMSGGV
                     AYVLNEDGDFQKRCNLSMVALEPVLAEVAAAKAAGEEVPESHGRVHFNHLNKADEHAL
                     REQIEKHALHTGSERARAILENWSAYLPKFTKVMPTEYRRALQEMAAQKKEAA"
     gene            10381..>11509
                     /locus_tag="VFW59_00530"
     CDS             10381..>11509
                     /locus_tag="VFW59_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX5362731.1"
                     /translation="MGKPTGFMEFERISEGYAPVEKRLKHYKEFVAHLTDEQAKTQGA
                     RCMDCGVPFCTNGCPVNNIIPDWNDLVYRGNWREALDVLHSTNNFPEFTGRICPAPCE
                     AACTVNFNGDAVGIKSIEHAIIDKAGENGWVVPQPAAKKTGKKVAVVGSGPAGLAAAQ
                     QLARAGHAVTVFEKNSRIGGLLRYGIPDFKLEKGLIDWRMAQLEAEGVKFKTNTYVGK
                     ALPDAGVANDAKKTVSPDELLKKYDAVIMSGGSEQPRDLAMPGRELQGVHYALEFLIP
                     QNKEVAGDGKNPISAKGKHVVVIGGGDTGSDCVGTSNRHGAASITQIEVMPRPPEQED
                     KYAVWPNWPLKLRTSSSHDEGCKRDWAIATKEFIGENGVLKA"
BASE COUNT         2422 a         3111 c         3710 g         2266 t
ORIGIN      
        1 actgatgcat ctgttgttgg cttcggcgct cttgacggga tgcgccgggg aagagtttca
       61 ggatctgcgt gacttcgttc agaatgcggg agcagatttg cgcggcaaga taccaccccc
      121 tccggaagtg aagccgtacg agccctttgt ctatgacaat gtggataact tgcaggatcc
      181 attcaagccc cgcaagcccg actcgcgttc cgcgagccgt ccgggtttga atcagccgga
      241 tatggaccgt ccaaaagagg ccttggagga gttccctctg gaggggttgc acatggtcgg
      301 ttatctccgt caaaacaagg ttggatatgc cattattcgc gcccccgatg gcaagttgca
      361 ccgggtcagg gcgggtaact atgtcgggat gaacttcggt ttgattaaag aggtgggcga
      421 aaccgaaatc gtggtgcaag aaacggtgca ggacagcgcc ggtgactggt ccgagcggat
      481 aagtagcctg cagttggtcg aataatttgc gagtgttgcg gaagggcgat ctctgacgag
      541 atcgcggcca cgagttatca atgtttgatt ccggtaatgc cggtttagga gcgaagcaat
      601 gaaacctttt aattccgtcc ttagaggcat ggcgcaggtg ctgggactgt tcgtcgcact
      661 gggcatgtcg gcagcgcagg ctgaagtgca aaacagcatc accgctttag gtgtcagtag
      721 cgcaggcaat ggagcaacgg tcgttaaagt cgaatttgcg cagccattaa caagcttgcc
      781 gcacgatttt acgatcaata ctcctccccg catcgcgctg gatttctcca atacggcaaa
      841 cgggctgggt aaatcgactc aggaatttac cgagggtgag cttcgcagcg ctaacatcgt
      901 gcaggcagga acccgtacgc gtctggtgat caatctgaat cagatgctca cctatagcac
      961 caaaattgat ggcaataact tgctggttac gttgcagggt aaggtggctg ccaatgctga
     1021 caccggtagt acctcacgct tcgccgaagt taaacagagt gcacagaaac attcattgcg
     1081 cgatatcgac ttccgtcgcg gcaagaacgg cgaaggccgc attcaggtcg atctttccga
     1141 tgccggcgtg ggtatcgaca ttcgtcagca aggaacgacg ctggtcgttg acttcctcaa
     1201 gtccagcctg ccgaagaatc tgcaacgcaa gctggatgtg gtcgattttg ccaccccagt
     1261 gcagggcgta gacacttttg tgcagggtga caatgtgcgt atggtgatcg agcccaaggg
     1321 gctatgggag catgcggcct atcagacaga caacaagttt atcgtcgaag tgaagccaat
     1381 cgccgacgac cctaacaaac tggtgaaagg aagtcaggct ggctatgccg gcgagaagtt
     1441 gacgctgaat ttccagaaga tcgatgtgcg tgaagcgctg aatgtgatcg cggatttcac
     1501 cgagttgaac atggtgatca gcgataccgt gagcggtaac ctgacgctgc gtttaaagga
     1561 tgtgccctgg gatcaggcgc tggatatcat cttgcagagt cgcggcctgg atatgcgcaa
     1621 gaatggcaac gtgattcagg tcgctccgcg agatgagatt gctgcgaagg agaagattga
     1681 tctgacctcg cgtcaggaaa tctccgatct cgaggagttg cgcacggaga gcttccagtt
     1741 gagctaccag aagggcgatg cggtaaccag gctgttgtcg ggagacaaac agcgcatcct
     1801 gtccaagcga ggtagcacgg tggttgatcc gcgtaccaac acgctgtttg ttcaagacac
     1861 cccgtcccat ctcgacgagg tgcgcaaact gatcaagcaa atcgacgtgc cggtgcggca
     1921 ggtgatgatt gaagcgcgtt tcgtttctgc taccgatgat ttcaaccgta ccctgggtgg
     1981 aaaattggga tataccggcc cggcagttgg tgtgggtaac ggttttggca tcggcgggag
     2041 gccgaattcg acacaaggcg tcatcggcgg taacgtgaac ttgccgggcg cggcatctgg
     2101 tacgggcggc ttgacgcttt ccttgttcaa tccggctgca accaaggtgc tgacgctgga
     2161 gcttaccgcc tcggagctgg atggcaagac caagaatatt gcgagtcctc gcgtggtgac
     2221 ggcagataag actcaggcaa agatatcggc aggcacgcag atcccgtatc agcaggcttc
     2281 aagcagcggt gcgaccgccg ttgctttcaa ggatgcgacg ctcagtctca ccgtgactcc
     2341 gcaaatttcg ccggacgaca atgtcgatat gaagctggat gttaatcagg acactgtggg
     2401 tgccatatat ggcggcgtgc caagcatcaa taccaagcaa gtgagcacgc aggtgatggt
     2461 cgagaatggt ggtacggtcg tgatcggcgg agtctttacg cagaacatcg gcgattcgac
     2521 acagaaggtg ccggtgctgg gcgatgtgcc ggtgctgggc tggttgttca agaacaatgt
     2581 gaagagcgac accaagagtg aattgctgat ctttatcacg cccaagatcg tgaaagatgc
     2641 gatgaatctg cattgatgca ttgaaaatgc ttcagcaaaa gcccggcaga gtccgggctt
     2701 tttgttttat cgtttacaat gcgcgccatg catagcgatc acgcaaacag tcgcccgaag
     2761 ggcggtcgta agttcaattc gggcaacctg attctggtgg gtttgatggg ctcgggaaaa
     2821 accaccatgg ggcgagctct ggcgaagcat ctgggaaaga ccttcgtgga tagcgatgac
     2881 gaggttcagc agcgtactgg cgtgaccatc ccccatattt tcgatatcga aggggagtcg
     2941 ggatttcgtc agcgcgaaac cgctgctatc cgggaattgg tcgagcgcga tgacatggtg
     3001 ttggcaaccg gcggcggtgc ggtgctggca gaagagaatc gagtgctctt gcagcagaac
     3061 ggcatcatcg tctaccttaa agccaacgtc catgacttat ggctgcgcac gcgccatgat
     3121 cgcagccgtc ccctgctgca gacaggcgac ccgcgtgcga aattgacgga actctttcag
     3181 caacgcgatc ccttgtatca gcaggtcgcg gatatcacgg tgcagagcgg caaacaaacc
     3241 gtccatggtc tgatgctgta tctggtgaat gaaatcgagg cgttcaaggc gaatgatgca
     3301 tcgcaggggc gagtcatatc gggccacggc atagagcacg aacaatccaa aatttaacgg
     3361 aagaacgatg cagacattga cagtaggttt gggcgagcgc agttacccga tacatatcgg
     3421 cagtggcctg ttggcgcagc ctgctttgct gcaagcgcat ttgccgcgca agcgggccgc
     3481 tatcgtgacc aatacaacgg tggcgccgct gtatctggac aagttgcagc agactttgca
     3541 ggctataggc gtgaatagca tctcggtggt tctgccggac ggtgaagagc acaagaatgc
     3601 agagacactt aatctgatct acgacgcgct gctggctaac cgttgcgaac gcagtacgcc
     3661 gctgattgca ctgggtggcg gggtgatcgg tgacatgaca gggtatgctg cggcgagcta
     3721 tttgcggggc gtgccgttca ttcaaattcc gactaccttg ctgtcgcagg tggattcttc
     3781 ggtgggcggc aagaccggca tcaatcatcc gctgggcaag aacatgatcg gcgcgttcta
     3841 ccagccgcag ctggtgctgg ccgataccga caccctgaat acgctgccag acaacgagct
     3901 tgcggccggt ctggccgagg tcatcaagta tggtctgata agagacctgc cgtttctgga
     3961 atggctggag cagaacatgg acaagctgct ggcgcgtgac acgacggcat tgcaatacgc
     4021 tatcgcgcgc agttgtcaga acaaggccga ggtggttgcg gctgacgagc gcgaaagcgg
     4081 ggagcgggcg ttgttgaacc tggggcacac cttcggccat gccatcgaat cggggatggg
     4141 ctatggcaac tggcttcatg gtgaaggggt cgcggccggt accgtgatgg cggcggatct
     4201 gtcgcagcgg ctgggatgga tcgatgcgcg ggacgtggcg cgcatccgca agctgttcga
     4261 gcgtgccagg ctgccggtta tttctcctga tttgggcacc gaaaaatatc ttgatctgat
     4321 ggggctggac aagaaggttg agggaggcaa ggtgcgtttc gtgctgctca gggagctcgg
     4381 tcgcgcggta atagacggcg acgtgccgcc agcgctgctg cagcagactt tggaggcctg
     4441 tgtccgtggc tgactttgcg ccctacagcg tcagcgaagc caattcgcgc ggacgcaaga
     4501 tcgcggaggc ggcaccagcg gggcgcggtg aatttcagcg cgaccgggat cgcatcatcc
     4561 attcgagcgc gttccggcgt ctcgagtaca agacgcaggt attcgtgaat cacgagggcg
     4621 acctgttccg cacccgtctg acccatagca tcgaggtggc ccagatcgca cgttctatcg
     4681 cgagacggtt gcatctgaac gaagatctcg cggaggcgat ttcgctggcg cacgatctcg
     4741 gtcatacgcc attcggtcat gccgggcagg acgccctgaa tgcatgtatg caggcttacg
     4801 gcggcttcga gcataacttg cagtcgttgc gcgtggtcga tctgctggag gaacgctatg
     4861 ccgaattcga ggggctgaat ctgaccttcg agacgcgcga gggcatcctc aaacattgct
     4921 ctcctgacaa tgctgcgcgt ctgggtgagt tgggtgagcg tttcctgaat gatcgccgtc
     4981 cctcactgga ggggcagatc gccaacctgg ccgacgagat cgcctataac aaccacgacg
     5041 tggatgatgg tctgcgctcc gggttgatca cgttggaaca gctggccgaa gtgccgttgg
     5101 tggcgcgtca tctggacgaa gtgcgtgccg cctatcctgc gctgccggag cggcgtgtgg
     5161 tgcatgagac cgtgcgccgc atgatcaaca cgctggtgtc cgacctgatc cggcaaagcg
     5221 agtcgaacat cgccgcacag aaactggaga cattggaaga cgtgcatcgc gcgccggtgc
     5281 tgattgcctt cagcgatgaa atgaacgcgc agcaacgggt cttgaaaggt tttttgcgtg
     5341 tcaatctgta tcgccactac cgggtgatgc gcatgagcgc gaaggcgcag cgcatcatca
     5401 gcgacctgtt cggggtgttc atgggcgacc ctcgcctgct gcctccgcag ttccagcagc
     5461 gcgagggtaa cggcgaagcc gagcgtgccc gtttggtggc cgattacatc gccgggatga
     5521 ccgatcgcta cgcgattcgg gagcatcggc gtatcttcgc cgtggaagag gcgtaagccc
     5581 gtcaatcatc ggggattccg ggctgcggtt gccccaagcc ctcagacaag gtaagattcc
     5641 cgccctttcg cttttgtatc tgacgtggcg acatgtgtcg ccattttttc ttttattgat
     5701 ttgaccgagg aacgccgtga gtaacagttc tttaccgatg caacaggggt tgtacgatcc
     5761 gcgccaagag cgcgatgcct gtggggtggg ttttgtcgcc catatcaagg gaaaaaagag
     5821 ccatgacatg gtgcgtcagg gcttgcagat tctggagaat ctgacgcacc gtggtgcggt
     5881 cggagcggat ccgctggccg gcgacggcgc cggcatcctg attcagattc ccgatcagtt
     5941 cctgcgcgaa gagatgggct gggggaatat ccagttgccc gccgtaggga gctatggtgt
     6001 gggcatcatg ttcctgccgc gcgatgcggc ggcgcgtgcc gcctgcgagc agatcgttgc
     6061 cgacaagatc gcagccgaag gccagtctgt gctgggttgg cgcgatgtgc cggtggatgg
     6121 ttccggattg ggcgagagcg tcaagaaggt cgagccggtc gtgcgtcagg tgttcatcgc
     6181 gcgcggcgag aattgcgccg atgtcgatgc gttcgagcgc aagctgttcg tgatccgcaa
     6241 gacggtcgag catgcggtgc gtggtttggg cagcaatcag gggacgggtt tctacatccc
     6301 ttcgctgtcg gcccgcacca tcgtttacaa gggaatgctg ctggccgatc aggtcggcaa
     6361 atactatctg gatctgaaag atgagcgcgt ggtcagcgcg ctggcgctgg tgcaccagcg
     6421 tttctccacc aacaccttcc cgacctggga tctggcgcat ccgttccgca tgattgcgca
     6481 caacggcgag atcaatacgg ttcgcggcaa cgtgaactgg atgaccgcgc gccatgccgc
     6541 gatgtcctcc cagctgctgg gcgaagatct ggaaaagctc tggccgctga tcgtcgacgg
     6601 tcagtccgat tcggcctgtt tcgacaatgc gctggaattg ctggtggcgg gcggttactc
     6661 cttgcctcac gcgatgatgt tgctgatccc ggaagcatgg gccggcaatc cgctgatgga
     6721 cgaagagcgc cgcgcgttct acgaatacca tgccgcgctg atggagccgt gggacggccc
     6781 cgccgccgtg gcattcaccg acggccgcat gataggcgcg acgctggatc gtaacggcct
     6841 gcgtcccgcg cgttatctga tcaccgacga cgacatggtg ctgatggcgt ccgagatggg
     6901 cgtgctggat attccgcagc acaagatcgt caagaaatgg cgtctgcagc cgggcaagat
     6961 gttcctgatc gacatggaag ccggacgcat cgtggacgat gtcgagctga agacgcagct
     7021 ttccaccgcc aagccgtacc gccagtggat cgaaaaatcg cgctatttcc tgggtgatct
     7081 gcccaaggcc aagggcgacg tcaaactcaa tgcctcgttg ctggatacgc agcaggcttt
     7141 cggttactcg caggaagacc tcaagttcat tctggcaccg atggtgaatg cgggcgaaga
     7201 gccgaccggt tcgatgggta tcgacgcggc gctgccggtg ctgtcgaaca agaaccgttc
     7261 gttctacgac tacttccagc agctgttcgc gcaggtgacc aatccgccga tcgacccgat
     7321 ccgcgaagaa atcgtgatgt cgctgacctc gttcatcggg cctaagccca acctgctcgg
     7381 gatcgacgag accaatccgc cgctgcgtct ggaagtgcat cagcccgtgt tgtcgaacga
     7441 tgacatggtt aagctgcgca atatcgacaa gctgaccaag ggcgaataca agtctcaggt
     7501 tctggatatc acctatccgg ccaagaacgg cgcggaaggt tgcgaggccg caatcagggc
     7561 gctgtgtgct gcggccgaca aggcggtggc tggcggttac aacgtgttga tcctgtcgga
     7621 tcgcgagatg tcggaaaagc gcgtggcgat tcccgcgctg gtggcctgcg ccaaggtgca
     7681 tcaccatctg gtcagcgaag gcttgcgcac cagcgcaggt ctggtggtgg acaccggctc
     7741 tgcgcgcgaa gtgcatcact tcgcgctgct cgccggttac ggcgcggaag cggtttgccc
     7801 gtggctggta tacgaaacca tcgcggacat gaagttgccc gcgaacgtcg atgccaaaga
     7861 agccggcaag cgtttcatca aggcgatcaa caagggcctg atgaaagtga tgtccaagat
     7921 gggcatctcg acttaccagt cctactgcgg cgcgcagatc tttgaggcga tcggcctgaa
     7981 tgcctcgttc gtcgcgcagt atttcaccgg cacggcaacg cagatcgagg gcatcggcct
     8041 gaaagaagtc gccgaggaag cggtgcgcac ccatcgcgcc gctttcggcg ccgatccggt
     8101 gctggccacc gcgctggatg cgggcggcga atacgcctat cgtgtgcgcg gcgaagagca
     8161 catgtggacg ccggattcca tcgccaaact gcagagcgcg acgcgcacca acaatgccgc
     8221 gacctacaag gaatacgcca agctgatcaa cgagcagagc cgcaagctga agacgctgcg
     8281 cggcatgttc gagatcaagc cggtcggcgc gccggtgccg ctcaatgaag tggaaccggc
     8341 caaggagatc gtcaaacgtt tcgtgaccgg cgcgatgtcg ctgggttcga tctccaccga
     8401 ggctcacacc acgctggcga tcgcgatgaa ccgcatcggc ggcaagtcca ataccggcga
     8461 aggcggtgaa gatgccgcac gtttccgcac gctgaaagcg ggcgagaagc tgtcggacat
     8521 catcggcaag aatcgcatcg agaaagactt cgtgatgaag gagggcgatt cgttgcgctc
     8581 gcgcatcaag caggtcgcct cgggccgttt cggcgtgaca gcggaatacc tgtccagcgc
     8641 cgatcagatc cagatcaaga tggctcaggg cgcgaagccc ggcgaaggcg ggcaattgcc
     8701 gggcggcaag gtgtccgagt acatcgccaa gctgcgcttc tcggtgccgg gcgtggggct
     8761 gatctcgcct ccgccgcatc acgatatcta ttccatcgaa gacttggcgc agctgatcca
     8821 tgacctgaag aattccaatc cgacggcttc gatctcggtc aagctggtgt ccgaagtggg
     8881 cgtgggtacg gtggctgcgg gtgtatccaa ggccaaggcc gaccacatcg tggtgtccgg
     8941 ttttgacggc ggcaccggcg cttcgccgct gtcctcgatc aagcatgcgg gtacgccgtg
     9001 ggagctgggt ctggccgagg cgcagcagac gctggtgctg aaccagttgc gcggccgtat
     9061 cgcgctgcag gtggacggcc agctgaagac cggccgcgac gtgctgatcg gcgcattgct
     9121 gggcgcggac gagttcggtt ttgcgaccgc accgctggtg gtggaaggct gcatcatgat
     9181 gcgcaagtgc catctgaaca cctgcccggt cggcgttgcg acgcaagacc cagagttgcg
     9241 taaaaaattt acagggcagc ctgaacatgt cgtcaattac ttcttcttcg tcgccgaaga
     9301 agcgcgcgaa ttgatggcgc agatgggtat ccgcaagttc gacgacctga tcggccgttc
     9361 cgatctgctc ggcgtgaaag aggggatcgc gcactggaag tcgcagggcc tggacttctc
     9421 ccgtgtgttc catcagcccg acatgcccgc cagcgtggcg cgtcgtcatg ccgaggcgca
     9481 ggatcacgag ctgaacaagg cgctggataa caccctgatc gccaaggcgc agggcgcgtt
     9541 gaaggacaag aagccggtgg tgatcgagag ccgggtcggt aacgtcaatc gtaccgtcgg
     9601 caccatgctg tcgcatgaag tggccaagcg ttacggccag gccggtttgc cgagcgacac
     9661 catccaggtg aagttcaccg gcacggccgg acagagcttc ggcgctttcc tcgcgcacgg
     9721 catctccttc gagttgcgcg gcgaaggcaa cgattacgtt ggcaagggac tgtgcggcgg
     9781 acgcatcgcc atcaggcccc ctgcagaagc caacctggtc gcgcacgaga acaccatcgt
     9841 cggcaatacc gtgttgtacg gtgcgacttc cggcgagtgc tatttcaacg gcgtggcggg
     9901 cgagcgtttc gcggtgcgca actccggcgc catcgcggtg gtggagggcg tgggcgatca
     9961 cggttgcgaa tacatgaccg gcggcatggt catcgtgctg ggtcagaccg gccgcaactt
    10021 cgccgccggc atgtcgggcg gcgtggctta cgtgctgaac gaagatggcg atttccagaa
    10081 acgctgcaac ctgtccatgg tcgcgctgga gccggtgctg gcagaagttg ccgcagcgaa
    10141 ggccgcaggc gaagaggtgc cggagagcca tggtcgcgtg catttcaacc acctgaacaa
    10201 ggcggacgag catgcgctgc gcgagcagat cgagaagcat gcgctgcata ccggcagcga
    10261 acgcgcccgt gccattctgg aaaactggtc cgcatatctg cctaagttca ccaaggtgat
    10321 gccgaccgaa tatcgtcgtg cgttgcagga aatggcggcg caaaagaagg aggccgcata
    10381 atgggcaagc caactggatt catggagttc gagcgcatca gcgagggcta tgcgcctgtt
    10441 gaaaaacgcc tgaagcatta caaggagttc gtcgcgcatc tgaccgatga gcaggcgaag
    10501 actcagggcg cgcgttgcat ggattgcggc gtgccgttct gcaccaatgg ttgtccggtc
    10561 aacaacatca tccctgactg gaacgatctg gtgtatcgcg gcaactggcg cgaggcgctg
    10621 gacgtgctgc attcgaccaa caacttcccg gagttcaccg gccgcatctg tcccgcaccc
    10681 tgcgaggcgg cctgtacggt caatttcaac ggcgacgcgg tgggcatcaa gtccatcgag
    10741 cacgcgatca tcgacaaggc cggcgagaac ggctgggtcg tgcctcagcc tgcggcgaag
    10801 aagactggca agaaagttgc cgtggtcggt tcgggtcctg ccggtctggc ggcggcgcaa
    10861 caactggcgc gcgccggaca tgcggtcacc gtattcgaga agaacagccg catcggcggt
    10921 ctgctgcgtt acggcattcc cgacttcaag ctggagaagg gcctgatcga ctggcgcatg
    10981 gcgcaactcg aggccgaagg cgtcaaattc aagaccaata cctatgtcgg caaggcgctg
    11041 cctgatgcgg gtgttgcgaa cgatgcgaaa aagacggtat cgccggatga gttgctgaag
    11101 aaatatgacg cggtgatcat gtcgggcggt tcggagcagc cgcgcgatct ggccatgccg
    11161 ggccgcgagt tgcagggcgt gcattacgcg ctggaattcc tgattccgca gaacaaggaa
    11221 gtcgccggcg acggcaagaa tccgatctcg gccaaaggca agcatgttgt cgtcatcggc
    11281 ggcggcgata ccggttccga ttgcgtcggc acatccaatc gccacggtgc agcctcgatc
    11341 acccagatcg aagtgatgcc gcgtccgccc gagcaggagg acaagtatgc ggtgtggccg
    11401 aactggccgc tcaagctgcg cacctccagc tcgcacgacg aaggttgtaa gcgcgactgg
    11461 gcgattgcca ccaaggagtt catcggcgag aacggtgtgc tcaaggcga
//
LOCUS       DASUIV010000014         3589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_654232, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000014 DASUIV010000000
VERSION     DASUIV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654232"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFW59_00535"
     CDS             complement(<1..341)
                     /locus_tag="VFW59_00535"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294020.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5362732.1"
                     /translation="MSGNESNKQTGAQKTARNPIKIVPLQERLRKPQWIRVKAGSGQG
                     YNDVKRMLREHELHTVCEEASCPNIGECFGKGTASFMILGDVCTRRCPFCDVAHGKPL
                     PPDADEPANLAR"
     gene            complement(508..1605)
                     /gene="nadA"
                     /locus_tag="VFW59_00540"
     CDS             complement(508..1605)
                     /gene="nadA"
                     /locus_tag="VFW59_00540"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX5362733.1"
                     /translation="MPDNIIPIAFDQPDAAQRSCSTAQAWAKRPQEPSTEQKAELIAR
                     IKRMLKEQDAVLVAHYYVHPDLQDLAEATGGIVSDSLDMANFGHQHPAKTIVVAGVRF
                     MGETAKILNPEKRVLMPDLEAECSLDLGCPADEFAAFCDAHPDRTVVVYANTSAAVKA
                     RADWMVTSSIALPVVKHLMERGEKILWAPDRHLGGYVQEQTGADMLLWQASCIVHDEY
                     KAQELIELKAQHPGALVLVHPESPRAVVKLADVVGSTTQLIKAAQDSAAREFIVATDN
                     GILHKMRTLCPDKLFLEAPTAGQGANCTSCSHCPWMAMNGLINLAEVLETGKNEILVD
                     PAIIPRAVQPIRRMLDFAQQIKLPTRGIGNA"
     gene            complement(1595..2074)
                     /gene="nudB"
                     /locus_tag="VFW59_00545"
     CDS             complement(1595..2074)
                     /gene="nudB"
                     /locus_tag="VFW59_00545"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX5362734.1"
                     /translation="MTRYKQPVSVLVVIYTDPSTSSGQVQDMKVLLLERADHPGYWQS
                     VTGSCEPGEVLRDTAIRELREETGLDAQQSALTDWQSHNVYEIYPHWRHRYAPGITHN
                     TEHVFGLQLPAPVDIRLAPREHLNFQWMSWREAADKVFSPSNRAAILQLPERAIHAR"
     gene            complement(2078..2476)
                     /locus_tag="VFW59_00550"
     CDS             complement(2078..2476)
                     /locus_tag="VFW59_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX5362735.1"
                     /translation="MNSLARQLNDIAAAGSYTMTCSVDELRAAVHEANFALFDADLKG
                     VKGKQNLFNALALAAAFPPEFGANWDALADDLCDLSWCGKVSGYVLLLRNVSDTFGLS
                     ANDREIAQDLLADTVVYWRQRNKAFWIFFI"
     gene            complement(2479..2829)
                     /locus_tag="VFW59_00555"
     CDS             complement(2479..2829)
                     /locus_tag="VFW59_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEX5362736.1"
                     /translation="MSRHLRLGLLALLLWLPLAQAGQIELSVVMRAELPVEARVTLRA
                     IEQGGPFAYDRDGVVFKNYEHVLPQHSRGYYHEYTVKTPGARNRGARRIVCGPLPECY
                     YSPDHYRTFKRIRE"
     gene            complement(2836..>3589)
                     /locus_tag="VFW59_00560"
     CDS             complement(2836..>3589)
                     /locus_tag="VFW59_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5362737.1"
                     /translation="KGLAYIKVNDVTQLNETGLQSPIVKNIHEAALKTIVERTGAQNG
                     DIIFFGADKTKIVNDALGALRSKIGHDKGYVTGDKWKPLWVVDFPMFEYDDEARRWTA
                     CHHPFTSPKDEHLEFLETDPGRCLAKAYDLALNGWELGGGSVRIHKEEVQSLVFRALN
                     IDAEEAQLKFGFLLDALQYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKTQRAQC
                     LLTHAPSEVDERQLRDLHIRLRQQPAVEVTKE"
BASE COUNT          654 a         1145 c         1058 g          732 t
ORIGIN      
        1 cgcgcgagat tcgcaggctc gtcggcatcc ggcggcagcg gcttgccgtg cgccacatcg
       61 cagaacggac agcggcgtgt gcacacgtcg cccagaatca tgaagctcgc cgtgcccttg
      121 ccgaagcatt cgccaatgtt gggacaggag gcctcctcgc acaccgtgtg caactcatgt
      181 tcgcgcagca tgcgcttgac gtcgttgtac ccctgtccgc tgcctgcctt gacccgtatc
      241 cactgcggct tgcgcaggcg ctcctgcagc ggcacgattt tgatcggatt gcgcgcggtc
      301 ttctgtgcgc cggtctgttt attgctctca ttaccgctca ttgcatgtcc ttaacgctca
      361 tggtgattga ctccaccccg aatgatgaaa cgccccgtag gagcgacttt agtcgcgata
      421 ggcacgctcc aactccacga tcccttcgcg gctgaagccg ctcccacaaa aaccggcgct
      481 cccacgagga ttgtttcaca ttaagtatca cgcgttgccg atgccgcgcg tcggcagctt
      541 gatctgttgc gcgaaatcca gcatgcgccg gatcggctgt accgcacgcg ggatgatggc
      601 aggatcgaca agaatctcgt tctttcccgt ttcaagcact tccgccagat tgatcaggcc
      661 gttcatcgcc atccacgggc aatgactgca actggtgcag ttcgcgccct gcccggcggt
      721 gggcgcctcg agaaataact tgtccggaca cagcgtgcgc atcttgtgca ggatgccgtt
      781 gtcggtggcg acgatgaatt cgcgtgccgc cgaatcctgc gccgccttga tcagctgcgt
      841 ggtggaaccg accacatcgg ccagcttgac cacggcgcgc ggcgactcgg ggtgcaccag
      901 caccagcgct cccggatgct gcgccttgag ttcgatcagt tcctgtgcct tatattcgtc
      961 atgcacgatg caggacgcct gccagagcaa catgtccgca ccggtctgct cctgcacgta
     1021 gccgcccaga tgacgatccg gagcccacag gattttttca ccgcgctcca tcaggtgctt
     1081 caccacgggc aaggcgatgg agctggtcac catccagtcg gcgcgcgcct tcaccgccgc
     1141 actggtattg gcatacacca ccacggtgcg gtcgggatgc gcatcgcaga atgcggcaaa
     1201 ttcgtccgcc ggacaaccga gatcgagcga gcattccgct tcgaggtcgg gcatcagcac
     1261 ccgcttctcg ggattcagta tcttcgcggt ctcgcccatg aaacgcacgc ccgccaccac
     1321 gatggtcttg gccggatgct ggtgaccgaa gttggccatg tccagcgagt cggacacgat
     1381 gccgcccgtt gcttccgcga ggtcttgcaa atcgggatgg acgtagtaat gcgcgaccag
     1441 caccgcatcc tgttccttga gcatgcgctt gatgcgcgcg atcagctcgg ccttctgctc
     1501 agtgctcggc tcctgcggac gcttcgccca ggcctgcgcg gtgctgcaac tgcgctgcgc
     1561 tgcgtcgggc tgatcgaaag cgatgggaat gatgttatcg ggcatgaatc gctctttccg
     1621 gtaattgcag gattgccgcg cggttgctcg gcgagaacac cttgtctgcc gcttcccgcc
     1681 agctcatcca ctgaaaattc agatgctcgc gcggggcgag ccggatatcg accggggccg
     1741 gtagttgcag gccgaacacg tgttcggtgt tgtgggtgat gccgggcgca tagcggtgac
     1801 gccagtgcgg atagatctcg tagacgttgt gcgactgcca gtcggtcagc gcagactgct
     1861 gcgcatccag ccctgtctcc tcgcgcagct cgcggatcgc cgtatcccgc agcacttcgc
     1921 ccggctcgca actgccggtg accgattgcc agtaaccagg atggtcggcg cgttccagca
     1981 gcagcacctt catgtcctga acctgtcctg agcttgtcga aggatcggtg taaatcacca
     2041 ccagcaccga gaccggctgt ttgtatcgcg tcatgcccta gatgaagaag atccagaagg
     2101 ccttgttgcg ctgccgccag tacaccaccg tgtcggcaag aagatcctgc gcgatctcgc
     2161 ggtcattggc ggacagcccg aaggtatcgc tgacattgcg cagcagcagc acatagccgg
     2221 acaccttgcc gcaccaggac aggtcgcaca ggtcatcggc caatgcatcc cagtttgcac
     2281 cgaactccgg cgggaaagcg gcggcaagcg ccagcgcatt gaacaggttc tgcttgccct
     2341 tgacgccttt gaggtcggca tcgaacaagg cgaagttcgc ctcatggacg gcagcacgca
     2401 gctcgtccac gctgcatgtc atggtgtagc tgcctgccgc agcgatgtcg ttcagttgtc
     2461 gtgccaacga gttcatgact actctcggat acgtttgaaa gtccggtaat gatcggggct
     2521 gtaataacat tctggtaacg gtccgcacac gatgcgccgc gcgccgcgat tacgggcacc
     2581 cggcgtcttc accgtgtact cgtggtaata accgcgcgaa tgttgcggca acacatgttc
     2641 ataattcttg aataccacgc cgtcgcgatc gtaagcaaac ggcccgccct gctcgatggc
     2701 acgcaacgtc acgcgcgcct cgaccgggag ttcggcccgc attacaaccg aaagttcaat
     2761 ctgcccggcc tgcgccagcg gcaaccacag cagcaatgcc agcagtccga gacgcaggtg
     2821 gcgtgacatc gccgcttatt ccttggtcac ttccacggcg ggctgctggc gcagacggat
     2881 atgcaaatcg cgcaactggc gctcatccac ctcgctcggc gcatgggtca acagacattg
     2941 cgcgcgctgg gtcttgggga aggcgatcac gtcgcggatc gactcggcac cggtcatcat
     3001 ggtgacgatg cgatccagac cgaaggccag accgccgtgc ggcggcgcgc cgtattgcag
     3061 cgcatcgagc aggaagccga acttgagctg tgcctcttcc gcgtcgatgt tgagcgcgcg
     3121 gaacaccaga ctctgtactt cttccttgtg gatacgcacc gaaccgccgc ccagttccca
     3181 gccgttcaat gccagatcgt aggccttggc cagacaacgc cccggatcgg tctccaggaa
     3241 ctccagatgc tcgtccttcg ggctggtgaa gggatggtgg caggccgtcc agcgacgggc
     3301 ttcgtcgtcg tactcgaaca tcgggaagtc caccacccac agcggcttcc atttatcgcc
     3361 cgtgacatag cccttgtcgt gaccgatctt gctgcgcaat gcgccgagcg catcgttgac
     3421 gatcttggtc ttgtccgcgc cgaagaagat gatgtcgccg ttttgcgcgc cggtgcgctc
     3481 gacgatggtc ttcagcgccg cttcgtggat gttcttcacg atgggcgatt gcaggccggt
     3541 ttcgttcagc tgcgtcacgt cgttgacctt gatgtaggcc aagcccttc
//
LOCUS       DASUIV010000015         8271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_189426, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000015 DASUIV010000000
VERSION     DASUIV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189426"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..731)
                     /gene="rsxG"
                     /locus_tag="VFW59_00565"
     CDS             complement(51..731)
                     /gene="rsxG"
                     /locus_tag="VFW59_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HEX5362738.1"
                     /translation="MAKASVYAALNLLFFALLGTVALASTYLLTRDKIAESVEKEKLK
                     LVAQVVPQRLFDNAIVQDALQLPPSELLGTDDVSTAYRARLNGEASAVVLQPIAPDGY
                     SGKIFLIVAIRSNGELSGVRVVSHRETPGLGDYIDLSKGPWIKNFDGLSHATRKEPDW
                     KVKKDGGAFDYVAGATITPRAVVKAVHKSLLYFEQNRDKLLADDAGARRAVPASEHPP
                     HAATAKEK"
     gene            complement(740..1783)
                     /gene="rsxD"
                     /locus_tag="VFW59_00570"
     CDS             complement(740..1783)
                     /gene="rsxD"
                     /locus_tag="VFW59_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HEX5362739.1"
                     /translation="MLFSPFISKPTSVSQIMLRVLLALIPGIALYVWYFGPAILISLT
                     LASMTALATESAMLKLRGRPVAPFIKDNSALLTAWLLALSIPPLAPWWLVVVGTAFAI
                     SVSKHLYGGLGSNPFNPAMIGYAVLIISFPVQMTHWVTPHGLGSVELHLLDQLRFIFS
                     GALPPGVTLDAITMATPLDTLKTQLHLDRSMQQIMEMPIYGRLAGHGSEAIALGYLAG
                     GLYLLLNRIISWHIPVFYLGSLFVTAGIFHLVYPAHYATPMFHWFSGAAMVGAFFILT
                     DPVTGPTTPRGKLIFAAGAGFLTYVIRVFGGFPDGMAFSTLLMNICMPLIDAYTQPKV
                     FGKKTKPADGGQP"
     gene            complement(1790..3439)
                     /gene="rsxC"
                     /locus_tag="VFW59_00575"
     CDS             complement(1790..3439)
                     /gene="rsxC"
                     /locus_tag="VFW59_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEX5362740.1"
                     /translation="MRTLFKFHGGVHPPTNKTQSNGRPIAQVALPSKLIIPLQQHVGN
                     SAKPVVEVGQRVLKGQIIGTPEGRLSSAVHASTSGTISAIDLQAVAHPSGLPDLCVTL
                     IPDGRDEWIAHEPRDWRALAHTELRHLLREAGVVGLGGAVFPSDMKLYSHKHKIATLV
                     LNGAECEPYITCDDLLMRERATDIVQGAQIMRELLHAGEVLIGIEDNKPEAIAAMRAA
                     VSAGGHERMEVIAVPTVYPGGGAKQLIRVLTGIEVPTGVRSTDMGVQCFNVGTAYGAW
                     RAIAHGEPMISRIVTVTGNVGEPQNFEVPFGMPVDELVAQADARPETIGYIMGGPMMG
                     FRLPSAQVGVVKATNCIIAMSEALFPPAPPALPCIRCTRCADACPADLQPQELYWFSK
                     SKNFGKAQAYHLFDCIECGACSYVCPSHIPLVQYYQFAKSEIHAREHDKQAADRARER
                     HEYREYRIEREKQEKAERLAAKERAAQQAAKPVEQPGAESAQQMTIQAAIDQAKEQAA
                     AVKPKNTADLSQEQLAEIAEIEARRARVRELAQSNVDHPKE"
     gene            complement(3439..3984)
                     /gene="rsxB"
                     /locus_tag="VFW59_00580"
     CDS             complement(3439..3984)
                     /gene="rsxB"
                     /locus_tag="VFW59_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEX5362741.1"
                     /translation="MLTAIVVMSVIALMLGAVLGFSAIKFHVQGNPLADKIDAILPQT
                     QCGQCGFPGCKPYADAIAEGVADINLCPPGGEEGIKKLSELLGVEFKPFGGAAQPKPR
                     AVAFIDENTCIGCTLCIQACPVDAIVGAAKQMHTIVAAECTGCELCLPPCPVDCISME
                     VIGEKIATWKREYPAFALKPM"
     gene            complement(4022..4597)
                     /gene="rsxA"
                     /locus_tag="VFW59_00585"
     CDS             complement(4022..4597)
                     /gene="rsxA"
                     /locus_tag="VFW59_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEX5362742.1"
                     /translation="MTDYLLIVIGTVFVNNIVMVKILGLCPFMGVSKKLEAAIGMGAA
                     TTFVLTLGSGSSYLIQHYLLGPDLAYLTTLSFIVVIAGIVQFTEMAVQKTSPDLYQVL
                     GIYLPLITTNCAVLGIPLLNVQAKHNFMESLLFGFGGAVGFTLVMILFAGLRERMEGA
                     DVPEIFKGSAIALVTGGLMSLSFMGFSGLVK"
     gene            complement(4684..6552)
                     /locus_tag="VFW59_00590"
     CDS             complement(4684..6552)
                     /locus_tag="VFW59_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX5362743.1"
                     /translation="MSDTHTKQKLSTLLLGAIGVVYGDIGTSPLYALKETFAGHHPLP
                     VVEANILGVLSVMFWTIMLLVSLKYVTIIMRADNRGEGGSLALLALVNELTSKRPATK
                     WFIATLGVFAAALFYGDSMITPAISVLSAVEGLNVIAPQLGHYVLPTTVAILTALFLV
                     QKHGTGAMGLAFGPIMIFWFATLGLLGVLSIAQSPQVLWALNPLYAYDFLRIDPWLSF
                     LALGSIVLAVTGGEALYTDMGHFGKFPIRVTWFSFVLPALVLNYFGQGALLLHNPQAV
                     ENPFYLLAPEWALIPMVLLATAATVIASQAVISGAFSVASQSVQMGFLPRMEIRQTSD
                     KAQGQIYVPLTNWTLYLAVIFLVLTFQSSSNLAAAYGIAVTGTMMIDTVLIAFVLVAW
                     RWSPLLVIPLIGTFFIVDFAFFSANAMKIPQGGWFPLGIAAMSFTVLTTWKRGRKLMF
                     DEIKRQSVPMQLLLDSADSVNRVQGTAVFLHGAGEGAPSALLHNLKHNQVLHERNVLL
                     TVVVEDKPYVTQGNRLLVEDMGKNFYRVRVFYGFMEMPDIPAALEQCSSWGLTFDMMT
                     TSFFVSRAVIVSSPNPGMMKWRERLFLVLSKNAMNAADFFKIPTNRVIEMGTRIEI"
     gene            6724..7074
                     /locus_tag="VFW59_00595"
     CDS             6724..7074
                     /locus_tag="VFW59_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362744.1"
                     /translation="MAKDPTDYRTVDFIAPGLHVTRGQVLMDRQRHIAIVAKKGRSCA
                     HLVRVKSGELKLTKLTARELVEEWSDADYPFDRAVAKLLELGRQHGITDAARLALEEL
                     VRSGREPTQQRLFG"
     gene            complement(7261..>8271)
                     /locus_tag="VFW59_00600"
     CDS             complement(7261..>8271)
                     /locus_tag="VFW59_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX5362745.1"
                     /translation="FLLPLVALATAATVIASQATISGAYSMTLQAMRMGYLPRLFIQH
                     TSDSQRGQIYIPAVNWMMLVGVIVLVFQFGSSGALAAAYGIAVSGTMIITTLLTLFIA
                     LASPGRTRALLLPALLVFALLELLFFGSNLTKVFHGGWMPLALGMFLFLLLSTWKRGS
                     TLVAEQRRKLDIPMSAFIAGTQPDVPRVQGTAVYLTADPTMVPSALFHNLKHFKVLHE
                     QTLFLHVSTADVPYIPQTERLKVTQLAPGLYDVALHFGFRQEVDIPKALSGLGAQGLE
                     LDSMNTTFFVARSNVVDGLGGMSSWRCALFSWMTRQSEGAASFFNLPPNQVVELGTKV
                     ML"
BASE COUNT         1573 a         2389 c         2706 g         1603 t
ORIGIN      
        1 gtttccacag gccgttgtgc aggatgtcct tgtattcttg caggctcatg ttatttctcc
       61 ttcgcggtgg ccgcgtgagg cgggtgttcc gatgcgggca cggcacgccg tgcccctgca
      121 tcgtctgcga gcagcttgtc gcggttctgc tcgaaataca gcagcgactt gtgtaccgct
      181 ttgaccaccg cgcgcggcgt gatggtggca cccgcgacgt agtcgaatgc gccgccgtcc
      241 ttcttcactt tccagtctgg ttctttgcgc gtggcatgcg acagaccgtc gaagttcttg
      301 atccacggac ctttcgacag atcgatgtag tcgcctagtc ccggcgtctc gcggtgggat
      361 accacgcgca cgccgctgag ttcgccgttg ctgcggatgg cgacgatcag gaatatcttg
      421 ccgctgtagc cgtcgggtgc gatgggttgc agcaccacgg cgctggcttc accgttgagg
      481 cgggcgcggt aggccgtgct cacgtcgtcg gtgccgagca attcgctggg cggcagttgc
      541 agcgcatcct gcacgatggc gttgtcgaac agccgttgcg gtaccacctg cgcgaccagc
      601 ttgagcttct ctttctccac gctctcggcg atcttgtcgc gggtgagcaa ataggtcgag
      661 gccagtgcta ccgtgcccag cagcgcgaag aacagcaggt tcagcgcggc atagacagag
      721 gcttttgcca tggtgcgtct catggctgac ctccgtcagc gggtttggtt ttcttgccga
      781 acaccttggg ttgggtatag gcgtcgatca gcggcatgca gatgttcatc agcagcgtgg
      841 agaacgccat gccgtccggg aagccgccga atacgcggat gacataggtg aggaagcccg
      901 caccggcggc gaagatcagt ttgccgcgcg gcgtggtcgg gcctgtcacc ggatcggtca
      961 ggatgaagaa cgcgccgacc atcgccgcgc ccgaaaacca gtggaacatc ggcgtggcgt
     1021 agtgggccgg atagaccaga tggaagatgc cggcggtgac gaacagcgag ccgaggtaga
     1081 acaccgggat atgccaggag atgatgcggt tcagcagcag gtaaagcccg cccgccagat
     1141 agccgagggc gatcgcctcg ctgccgtgtc cggccaggcg accgtagatc ggcatctcca
     1201 tgatctgttg catggagcgg tcgagatgca gctgggtctt cagcgtgtcc agcggtgtag
     1261 ccatggtgat ggcgtccagc gtcacgcccg gcggcaaggc gccggagaag atgaagcgca
     1321 gctggtcgag cagatgcagc tctaccgaac ccagtccgtg cggcgtgacc cagtgcgtca
     1381 tctgtaccgg gaacgagatg atcagcacgg cgtagccgat catcgcggga ttgaacggat
     1441 tgctgcccag tccgccgtac aggtgtttgg aaacgctgat cgcaaaggcg gtgccgacta
     1501 ccaccagcca ccagggggcc agcggcggaa tggacagggc cagcagccag gcggtgagca
     1561 gcgcgctgtt gtcttttatg aagggggcaa cggggcggcc gcgcagcttg agcatggccg
     1621 attcggtcgc cagtgcggtc atgctggcca gcgtgagcga gatcaggatg gccgggccga
     1681 agtaccagac atacagcgcg atgccgggaa tcaatgccag cagcactctg agcatgatct
     1741 gcgagacgct ggtcggcttg ctgatgaagg gtgagaaaag catggtggcc tattccttcg
     1801 gatgatcgac gttggattgg gcgagttcgc gaacgcgcgc gcgacgggcc tcgatctcgg
     1861 cgatctccgc cagttgttct tgcgagagat ctgcggtgtt tttgggtttg actgcggcag
     1921 cctgttcttt ggcctggtcg atcgccgcct gaatcgtcat ctgttgggcg ctctccgcgc
     1981 cgggctgctc aaccggtttg gccgcttgct gggcggcgcg ttccttggcg gcgagccgct
     2041 cggctttttc ctgcttctcg cgctcgatgc ggtattcgcg gtattcgtgg cgttcgcgcg
     2101 cccggtcggc ggcttgtttg tcgtgttcgc gcgcgtggat ctcgctcttg gcgaactggt
     2161 aatactgcac cagcggaatg tggctggggc agacatagct gcatgcgccg cattcgatgc
     2221 aatcgaacag atggtaggct tgcgccttgc cgaaattctt cgacttggag aaccagtaca
     2281 actcctgcgg ctgcaagtct gccggacagg catcggcaca gcgcgtgcag cggatgcagg
     2341 gcagcgcggg cggggcgggc gggaacagtg cttccgacat ggcgatgatg cagttggtcg
     2401 ccttgaccac gccgacttgc gccgacggca ggcggaaacc catcatcggg ccgcccatga
     2461 tgtagccgat ggtttccggc ctggcgtccg cctgggcgac cagttcgtcg accggcatgc
     2521 cgaacggcac ttcgaaattc tgcggctcgc cgacattgcc ggtgacggtg acgatgcgcg
     2581 agatcatcgg ttcgccgtgt gcgatcgcac gccaggcgcc gtaggcggtg ccgacgttga
     2641 aacattgcac acccatatcg gtggagcgca cgccggtcgg aacctcgatg ccggtcagca
     2701 cgcggatcag ttgcttggcg ccgccgcccg ggtagacggt cggtaccgcg atgacttcca
     2761 tgcgctcatg tccgcccgcg cttaccgccg cgcgcatcgc cgcaatcgct tccggcttgt
     2821 tgtcctcgat gccgatcagc acctctccgg catgcagcaa ttcgcgcatg atctgggctc
     2881 cctgcacgat gtccgtcgcg cgttcgcgca tcagcaagtc gtcgcaggtg atgtagggtt
     2941 cgcactccgc gccgttgagc accagcgtgg cgatcttgtg cttgtgcgag tacagcttca
     3001 tgtcgctggg gaacaccgcg ccgccgagtc cgacgacacc ggcctcgcgc agcaggtggc
     3061 gcaattcggt atgcgccagt gcgcgccagt cgcgcggttc gtgcgcgatc cattcgtcgc
     3121 ggccgtccgg gatcagcgtg acgcacaggt cgggcaggcc ggacggatgt gcgaccgcct
     3181 gcaggtcgat ggcgctaatg gtgccggaag tcgacgcgtg taccgcgctc gacaagcggc
     3241 cttccggcgt gccgatgatc tgtcccttga gcacgcgctg gccgacttcg acgaccggtt
     3301 tcgcgctgtt tccgacatgc tgctgcagcg gaatgatcag ctttgagggg agcgcaacct
     3361 gagcgatcgg cctgccgttc gactgggtct tgttggtcgg cgggtgtact ccgccgtgga
     3421 atttgaatag cgttctcatc acatcggctt cagggcgaaa gcgggatatt cccgcttcca
     3481 ggtggcgatc ttttccccga tgacttccat gctgatgcag tcgaccgggc agggcggcag
     3541 acacagttcg cagccggtgc attcggcggc gacgatggtg tgcatctgtt tcgccgcgcc
     3601 gacgatggca tccaccggac aggcctgtat gcacagggtg cagccgatgc aggtgttttc
     3661 gtcgatgaag gcgactgcac ggggtttggg ttgggccgcg ccgccgaacg gtttgaattc
     3721 gacgcccagc agttcggaca gtttcttgat gccttcttcg ccgcccggcg ggcacaggtt
     3781 gatgtcggcc acgccttcag cgatggcgtc ggcatagggt ttgcagccgg ggaagccgca
     3841 ttggccgcac tgtgtctgcg gcaggatcgc gtcgatcttg tccgccagcg ggttgccctg
     3901 cacgtggaat ttgatcgccg agaaacccag caccgcgccg agcatgaggg cgatgaccga
     3961 catcactacg atggcggtca gcatggggat acccgcagca gttgacgatt cacggcgtgc
     4021 attacttcac cagtcccgag aaccccatga aagaaagact catcaatccg ccggtgacca
     4081 gcgcgatggc cgaacccttg aatatctccg gtacgtccgc gccttccatg cgctcgcgca
     4141 gtccggcgaa caggatcatc accagcgtaa agcccacggc gccgccgaaa ccaaacagca
     4201 gcgactccat gaagttgtgt ttggcctgca cgttcagcag cgggatgccc agcaccgcgc
     4261 agttggtggt gatcagcggc aggtagatgc ccagcacctg atacagatcc ggactggtct
     4321 tctgtacggc catttcggtg aactgcacga tgcccgcaat caccacgatg aacgacaggg
     4381 tggtcagata cgcgagatcg gggccgagga ggtaatgctg gatcaggtag ctgctgccgg
     4441 aacccagtgt cagcacgaag gtggtggccg cacccatgcc gatggcggct tccagtttct
     4501 tggaaacccc catgaacggg catagcccga gaatcttgac catcacgatg ttgttcacga
     4561 acaccgtgcc gatcactatg agtaagtagt cagtcattgc ttgctcttaa aaacaagcgc
     4621 ggattatgcg ccgattcaaa taccccttca accgcgttaa cggcatatgg atattccctg
     4681 tggttagatt tcgatcctcg tccccatttc gatgacgcgg ttggtcggga tcttgaagaa
     4741 gtccgccgcg ttcatcgcat ttttggagag caccaggaac aggcgttcgc gccatttcat
     4801 catgccgggg ttcgggctgg agacgatgac ggcgcgcgag acgaagaacg aggtggtcat
     4861 catgtcgaag gtcagccccc aggatgagca ctgttccagc gcggcgggaa tgtccggcat
     4921 ctccatgaag ccgtagaaga ccctgacccg gtaaaaattc ttgcccatgt cttcgaccag
     4981 caggcggttg ccctgcgtga catagggttt gtcctcgacg acgacggtga gcagcacatt
     5041 gcgttcgtgc agcacctggt tgtgtttgag attgtgcagc agcgcggagg gcgctccttc
     5101 gcctgcgccg tgcaggaaga cggcggtgcc ctgcacccgg ttgacgctgt cggcgctatc
     5161 cagcagcagt tgcatcggca cggattgccg tttgatttca tcgaacatta atttccggcc
     5221 gcgcttccat gtggtcagca cggtgaagga catcgccgcg atgcccagcg ggaaccagcc
     5281 gccctgcggg atcttcatcg cattggccga gaagaacgca aagtcgacga tgaagaaggt
     5341 gccgatcagc ggtatgacca gcagcggcga ccagcgccag gccaccagca cgaaggcgat
     5401 caggacggtg tcgatcatca tcgtgccggt gaccgcgatg ccataggccg ccgccagatt
     5461 gctggaactc tggaaggtca gcaccagaaa gatgacggcc agataaagcg tccagttggt
     5521 cagcgggaca tagatctgcc cctgtgcctt gtcggaagtc tggcgtatct ccatgcgcgg
     5581 caagaagccc atttgcaccg actggctcgc gaccgagaat gcgccggaga tcacggcctg
     5641 cgatgcgatc accgtggctg cggtggccag cagcaccatc ggtatcagcg cccattccgg
     5701 agcgagcaga tagaacgggt tctcgacggc ctgcgggttg tgcagcagca gtgcgccctg
     5761 gccgaagtag ttcagcacca gcgcgggcag cacgaaggag aaccaggtga cgcgaatcgg
     5821 gaattttccg aagtgcccca tgtcggtgta cagcgcctcg ccgccggtca cggccagcac
     5881 gatggagccc agcgcgagaa aggatagcca gggatcgatg cgcaggaagt cgtaggcgta
     5941 gagggggttg agcgcccaca acacctgcgg gctttgcgcg atggacagca cgcccagcag
     6001 tcccagcgtg gcgaaccaga aaatcatgat ggggccgaac gcgagcccca tcgcgccggt
     6061 gccgtgtttc tgcaccagaa acaatgcggt cagtatcgcc accgtggtgg gaagcacgta
     6121 gtggccgagt tgcggcgcaa tgacattcag accctcgacc gcagagagca ccgagatcgc
     6181 gggggtgatc atgctgtcgc cgtaaaacag cgcggcggca aatacgccga gtgtcgcgat
     6241 gaaccatttc gtcgcggggc gtttcgaggt cagctcgttg accagtgcca gcaaggccag
     6301 cgagccgcct tcgccgcgat tgtccgcgcg catgatgatg gtcacgtact tgagcgaaac
     6361 cagcagcatg atggtccaga acatcaccga cagcacgccg aggatgttgg cttcgacgac
     6421 ggggagcgga tgatgtccgg cgaaagtctc tttcagcgca tacagcgggc tggtgccgat
     6481 gtcgccatag accaccccga tcgcgcccag caatagcgtg gataactttt gtttggtgtg
     6541 cgtatcgctc atcccgtccc tttttttgca gccttcttct cagtcctctt ctctgtggaa
     6601 gagtggggcg gcactctacg cctgcgcccc cccgaacgca atcgggcagt tctgttgccc
     6661 gggcgggcat ttttcgccca tacccgttaa gattgttgcc attcaggata ttgagttgga
     6721 tgtatggcga aggaccctac cgattatcgc accgtcgatt tcatcgcccc cggcttgcac
     6781 gtgacgcgcg gccaggtgtt gatggatcgg cagcggcaca tcgccatcgt ggcaaagaag
     6841 gggcgcagct gcgcgcatct ggtgcgagtg aagtccgggg agttgaaact gaccaagctg
     6901 actgcgcgcg agctggtgga ggaatggtcg gatgcggatt accctttcga ccgcgcggtg
     6961 gcgaagctgc tcgagctggg gcggcagcac ggcatcaccg atgcggcgcg cctggcactg
     7021 gaggaactgg tcaggtcggg gcgcgaaccg acgcagcagc ggttgttcgg ttaggtcctt
     7081 gcagatagcg gaagctagcg aagagcggct aacgacccgt tgctgaactt ggcatttcaa
     7141 gaatgcagat catcaacttc tggattggcc ggtgcggatt tttgtgcagg ggccggtgga
     7201 atgataggcc ggattggctc caggaggatt gattgccgcc tgctagccaa tgcggcaaga
     7261 ctacaacatg accttcgtgc ccagttcgac cacttggttg gggggcaggt tgaagaagct
     7321 cgctgcgcct tcagactggc gcgtcatcca cgagaacaaa gcgcagcgcc acgaggacat
     7381 gccgcctaag ccatccacca cgttcgagcg ggcgacgaag aaggtggtgt tcatactgtc
     7441 gagttccagg ccttgcgcgc cgaggccgct cagggctttg ggaatatcga cttcctgccg
     7501 gaaaccgaaa tgcagcgcga catcgtacaa gcctggggca agttgagtca cctttagccg
     7561 ttctgtttgc ggtatatagg gcacatcggc agtcgataca tgcagaaaca atgtttgttc
     7621 atgcaatacc ttgaagtgct tgaggttgtg gaacaacgcg ctgggtacca tggtggggtc
     7681 tgccgtcagg taaaccgccg tgccctgcac gcggggtaca tcgggctgcg tgccagcgat
     7741 gaaggccgac atcgggatat cgagttttcg ccgttgctcg gcaaccagtg tgctgccgcg
     7801 cttccatgtg gagagcagca ggaacaggaa catgccaagc gccagcggca tccagccgcc
     7861 gtgaaagact ttggtcaggt tggagccgaa gaacagcagt tccagcagag cgaacaccag
     7921 caatgcgggg agcaacagtg cgcgtgtccg tcccggtgaa gccagtgcga tgaacagcgt
     7981 caacagtgtg gtgatgatca tggtgcccga gacggcaatg ccgtaggcag cagccagtgc
     8041 gccggaggag ccgaactgga acacgagaac gatgacgccg accagcatca tccagttcac
     8101 ggcgggaatg taaatctgtc cgcgctgcga atcggaggtg tgctggatga acaggcgcgg
     8161 caggtagccc atgcgcatgg cttgcagcgt cattgagtac gcgccggaga tggtggcctg
     8221 cgaagcgatt accgtggcgg cagtcgccag tgccaccagc ggcagcagga a
//
LOCUS       DASUIV010000016         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_747514, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000016 DASUIV010000000
VERSION     DASUIV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747514"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..531)
                     /gene="ispF"
                     /locus_tag="VFW59_00605"
     CDS             complement(37..531)
                     /gene="ispF"
                     /locus_tag="VFW59_00605"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5362746.1"
                     /translation="MRIGQGFDVHQLVEGRKLIIGGVDIPYEKGLLGHSDADVLLHAI
                     CDALLGAAALGDIGRHFADTDAKFKNIDSRILLRETLHLVREQGFRVGNVDATIIAQA
                     PRMAPHIPKMVAHIAADLGLDASAVNVKATTTEELGFAGRREGIAAQAVCLIEALPPP
                     DSGG"
     gene            complement(534..1214)
                     /gene="ispD"
                     /locus_tag="VFW59_00610"
     CDS             complement(534..1214)
                     /gene="ispD"
                     /locus_tag="VFW59_00610"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5362747.1"
                     /translation="MSEFHALVPAAGFGARMGNALPKQYLPLAGQPMIFHALNTLCSC
                     PDISTVFVVLAPDDTLFHGYDWSRFGDKLQPLYCGGAMRADTVLNGLMVSEVEPDDWV
                     LVHDAARPCLTQAHLAKLIAELRDDAVGGILAVPVADTLKRADDAGRIARTEDRTGLW
                     QAQTPQMFRAGLLAQALQQCKAVTDEASAIEALGLQPKLVAGDSSNFKVTYPQDLLLA
                     ELLLQQGK"
     gene            complement(1262..>2746)
                     /locus_tag="VFW59_00615"
     CDS             complement(1262..>2746)
                     /locus_tag="VFW59_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5362748.1"
                     /translation="ERRERDALIAGHGLIWGLGIFGLFFGGRWQQRAIAEAELREAQV
                     TLLTNSIAHSIYGVDMQGCCTFINAAGVKAFGFDDASELLGKNMHDLVHHTRADGSPY
                     PHEACPSFHSIHDGKSFHLDNEVFWRKDGSFFPAAYWSYPVLQDGCPQGAVVTFIDIS
                     EQLRVKGELKHSQKMLNTIVENIPAMVFLKRAEDLRFELFNRAGEQLLGYSRDALLGK
                     SDHDLFPQDQADSFTRNDRAVLESRRPQEIPEEPIKTADGSDKWLDTFKIGLYDEADR
                     PTHLLGVSIDITARKLMQDELREQRRVLELALEGTIHTVSMAVEVRDPYTSGHQRRVA
                     ELACAIARIMGLDEERIKGILMGATIHDIGKIGVPAEMLSKPMQLTALEMQLIREHAM
                     MGFNILKDVRFPWPVAEIAHQHHERMDGSGYPQGLKGDAICLEARIVAVADVVESMAS
                     HRPYRPSLGLPAALDEVSRNRGLLYDAQVVDACRKVFDDGFEFK"
BASE COUNT          531 a          910 c          790 g          515 t
ORIGIN      
        1 cgtccgtcct cgcccagttt cgccgctgaa gcgggactag ccgcccgaat cgggaggcgg
       61 caacgcctcg atgaggcaca ccgcctgggc ggcaatcccc tcgcggcgcc cggcgaatcc
      121 cagctcctcg gtggtcgtcg ccttcacgtt cacggccgag gcgtcgaggc ccaggtcggc
      181 cgcgatgtgg gcgaccatct tcgggatgtg cggcgccatg cgcggcgcct gcgcgatgat
      241 ggtcgcatcg acattgccga cgcggaaacc ttgctcgcgc accagatgca gcgtctcgcg
      301 cagcaggatg cggctgtcga tgttcttgaa cttcgcatcg gtgtcggcga aatgcctgcc
      361 gatatcgccc agcgccgcag caccgagcag cgcatcgcag atcgcgtgca gcagcacgtc
      421 cgcgtcggag tggccgagca gccctttctc gtagggaata tctaccccgc cgataatgag
      481 cttgcgccct tctactaatt ggtggacatc aaaaccttgc ccgatacgca tgattatttc
      541 ccttgttgaa gcagcaactc cgcgagcagc aaatcctgcg gataggtcac cttgaaattg
      601 ctcgaatcgc ctgccaccag tttcggctgc aacccgagcg cctcgattgc cgaagcctcg
      661 tccgtgacgg ctttacattg ttgcagcgcc tgcgccagca ggcccgcgcg gaacatctgc
      721 ggcgtctgcg cctgccacaa tccggtgcgg tcttcggtgc gcgcgatgcg gcccgcatca
      781 tccgcgcgct tcagcgtatc cgccaccggc accgcgagga tgccgccgac cgcatcgtcg
      841 cgcagctctg cgatcagctt tgccagatgc gcctgcgtca ggcagggccg cgccgcgtca
      901 tgcaccagca cccagtcgtc cggctccact tcggaaacca tcaggccgtt cagcaccgtg
      961 tccgcacgca tcgccccgcc gcaatacagc ggctgcaact tgtcgccgaa acgcgaccag
     1021 tcgtaaccgt ggaacagcgt atcgtcggga gccagcacca cgaacaccgt gctgatgtcc
     1081 ggacaggaac acagcgtatt cagcgcgtga aagatcatcg gctgcccggc cagcggcaga
     1141 tattgtttgg gcaaagcatt gcccatgcgc gcaccgaaac cggcggcagg aaccagagcg
     1201 tggaattcag acatggcgcg agtttaccaa actcccgtgg tttgaatgcg gagacaacaa
     1261 ctcacttgaa ttcgaaccca tcatcgaaca ccttgcggca ggcatcgacc acctgcgcat
     1321 cgtagagcag cccccggttc cgggagactt cgtccagcgc cgcaggcaaa cccaggctcg
     1381 gacgataggg gcggtgcgaa gccatcgatt cgaccacgtc ggccacggcg acgattctgg
     1441 cctccaggca tatcgcgtcg cccttcagcc cctgcgggta acctgaaccg tccatgcgct
     1501 cgtgatgctg atgggcgatt tcggcaaccg gccaagggaa gcggacatcc ttgaggatgt
     1561 tgaaacccat catggcatgc tcccggatga gctgcatttc aagcgcggtc agttgcatcg
     1621 gcttgctgag catttcggca ggcaccccga tcttgccaat atcgtggatc gtcgcgccca
     1681 tgagaatgcc cttgatgcgc tcctcgtcca gtcccattat ccgggcaatg gcgcaagcca
     1741 gttcggccac gcgccgctgg tgtccggagg tataaggatc gcgcacctcg accgccatcg
     1801 acacggtatg gatcgtccct tccagcgcca attccagcac ccggcgctgc tcgcgcaact
     1861 cgtcctgcat cagcttgcgc gcggtaatat cgattgaaac gccgagcagg tgggtaggac
     1921 ggtccgcttc gtcgtacagg ccgattttga aggtatccag ccatttgtcg ctgccgtcgg
     1981 cggtcttgat cggctcctcg ggtatctcct gcggacggcg gctctccagc accgcacgat
     2041 cattccgggt gaaagaatcc gcctgatcct gcggaaacag atcgtgatcg ctcttgccca
     2101 gcagagcgtc gcgggaatag cccaacaact gctcgcccgc gcgattgaac agttcgaaac
     2161 gcagatcctc cgcgcgtttc aggaacacca tcgccggaat attttcgacg atggtattca
     2221 acattttctg cgaatgcttc agttcgccct tgacgcgcaa ctgctcgctg atgtcgatga
     2281 acgtcaccac ggcgccttga ggacagccgt cctgaagcac cggataagac cagtaggctg
     2341 ccgggaaaaa actgccgtcc ttgcgccaga atacctcgtt gtcgaggtgg aacgacttcc
     2401 cgtcgtgtat ggaatgaaaa ctcgggcaag cctcatgggg gtaaggcgat ccgtctgccc
     2461 gcgtgtggtg caccagatca tgcatgttct tgcccagcaa ctcggatgcg tcgtcaaagc
     2521 cgaaagcttt gaccccagcc gcgttgatga aggtgcaaca cccttgcata tccaccccgt
     2581 agatggaatg cgcgatcgag ttcgtcagca gggtcacctg cgcctcgcgc agctcggcct
     2641 ccgctatcgc ccgctgctgc catctgccgc cgaagaacag tccgaatata cccagccccc
     2701 agatcaggcc atgtccggca atcagcgcgt cgcgctcgcg ccgctc
//
LOCUS       DASUIV010000017         9561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_376221, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000017 DASUIV010000000
VERSION     DASUIV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376221"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2064
                     /gene="alaS"
                     /locus_tag="VFW59_00620"
     CDS             <1..2064
                     /gene="alaS"
                     /locus_tag="VFW59_00620"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX5362749.1"
                     /translation="IPGGLPGTPEEDGDRYIEIWNNVFMQFNRDEAGVMHPLPKPSVD
                     TGMGLERISAVLQHVHANYEIDLFQALIAAAARETNCSDLDSPSLKVLADHIRACSFL
                     IADGVIPGNEGRGYVLRRIIRRAIRHGYKLGARDAFFYRMVPDLVEQMGAAYPELVAE
                     QVRVKGILKQEEERFFATIEHGMAILEADLAGMDQAGVKVFNGETAFKLHDTYGFPLD
                     LTADICRERGVTVDDAAFNAAMANQKQMARSAGKFKMAANLEYEGPATTFHGYDTLEH
                     KGNVLALYKDGVSVNQLAEGEMGVVVLDDTPFYAESGGQVGDCGELRSVHGIFAVEDT
                     QKIQASVFGHHGTVTTGTLTVGNGVTAKVDVAARSRTMRNHSATHLMHKALREVLGTH
                     VQQKGSQVDADKTRFDFVHTQPISDEDIRKVEAIVNAEIFANVACQAREMAIEDAQKT
                     GAMMLFGEKYGDVVRVLNIGSSIELCGGTHVQRTGDIGLFKIVAESGVAAGVRRVEAV
                     TGEGALALVQQQEQLLQQVADAVKSQPQEAASRIAQILDNVKSLEKELAALKSKLASA
                     QGDELVAQAQDINGVKVLAAKLDGADAAALRETMDKLKDKLKSAAIVLASVTDGKVAL
                     IAGVTPDLTAKVKAGELVNVVAQRVGGKGGGRPDMAMAGGTQPEHLGQALSDVYAWVA
                     TRTQE"
     gene            2065..2523
                     /locus_tag="VFW59_00625"
     CDS             2065..2523
                     /locus_tag="VFW59_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5362750.1"
                     /translation="MVSTAARELLPVRNVLLIEDDPSLTEWVKQAAADFDTRIELTVL
                     PVGVAVLEWLGGVTRKRLPHIILLDLKLPKLDGLAVLRTLRSNVATRDTPIVVFSEEY
                     IQADVVLGYQVGANSFIAKPATQEACAECLREQLSYWLQPRQRELALGAQ"
     gene            complement(2562..3968)
                     /locus_tag="VFW59_00630"
     CDS             complement(2562..3968)
                     /locus_tag="VFW59_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX5362751.1"
                     /translation="MRELPSTEIFKAYDIRGIVGRTLTAETVEQIGQAIGSEAIARKQ
                     KSIVIGRDGRLSGPEFALALARGIRKSGIDVIDVGRVATPMVYFAAYQLGTGCAVMIT
                     GSHNPPDYNGLKMVLADETLYGEAIQRLRSRIETGDLAQGSGRYSQYDIAPEYLARIA
                     SDIRLARPMKISVDCGNGVAGDFAARLYRALGCDVTELFCEVDGNFPNHHPDPSDPRN
                     LADLIEALRDDTNLLSGHPNGSELGLAFDGDGDRLGVVARDGRIIYPDRQLMLFAADV
                     LERNPGAEIIFDIKSTGNLFAWIREHGGKPLLWKTGHSLVKAKMRETGALLAGEMSGH
                     VFFKERWYGFDDGLYAGARLLEILSRTADASATLNALPDACSTPELHIKLQEGESHAL
                     IERLAREARFTDAGEVIAIDGLRVEYEDGFGLMRASNTTPVIVLRFEADDEAALQRIQ
                     DDFRRVLLKANPALQLPF"
     gene            complement(4036..5049)
                     /gene="waaF"
                     /locus_tag="VFW59_00635"
     CDS             complement(4036..5049)
                     /gene="waaF"
                     /locus_tag="VFW59_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX5362752.1"
                     /translation="MYKILVIAPSWVGDCMLMQPMLQRLMQRHPDCRIDILAPPWTEK
                     LLRQMPEVSEVIVNPFPHGALQLGSRYRLGRQLRNARYDQAIVTPNSLKSALVPFFAG
                     IPLRTGFVGEMRYGLLNDARKLDKQRLPLMVERFAQLAEDKQGQIPRPLSNPALKVSA
                     AQRDAALNKLGLTLDRPVAAFCPGAEYGPAKRWPPAYYAELAKRLSTQGYAIWLFGSN
                     KDRAVADEIDELSGNACHNLCGRTDLGDAIALLSCAKLVVSNDSGLMHMAAALERPMI
                     ALFGSSSPQFTPPLSGRARVVQLDLPCSPCFKRECPLGHFNCMMQLTPDRVWQEIQPL
                     MTE"
     gene            complement(5110..5310)
                     /locus_tag="VFW59_00640"
     CDS             complement(5110..5310)
                     /locus_tag="VFW59_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX5362753.1"
                     /translation="MSNQTITQRYIEVTAHDLPLTCPMPNMSHWDAHPRVSIPLKNGG
                     EARCPYCGTLYKFKGELPRGGH"
     gene            complement(5321..6241)
                     /locus_tag="VFW59_00645"
     CDS             complement(5321..6241)
                     /locus_tag="VFW59_00645"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX5362754.1"
                     /translation="MSMSDRDGFIWYDGKIVPWRDATTHVLTHTLHYGMGVFEGLRAY
                     EAEKGTAIFRLQEHTDRLFNSAHIFQMKMPYDKETVMEAHREVVRLNKLKSCYIRPIA
                     FYGSEAMGIAATTLSTHVAVAAWPWGAYLGAEGMQKGIRVKTSSFTRHHVNVNMCRAK
                     SVTTYANSILAHQEVANDNYDEALLLDVDGYVAEGAGENLFIVKKGKLYTPDLTSCLE
                     GITRDSIITLAKEMGIEVIEKRITRDEIYCAEEAFFTGTAAEVTPIRELDQRTIGAGC
                     RGPVTERLQKAYFDCVAGKHPQHADWLSYV"
     gene            6425..7693
                     /locus_tag="VFW59_00650"
     CDS             6425..7693
                     /locus_tag="VFW59_00650"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5362755.1"
                     /translation="MAEKLTQSPDITAPAGNSQEDKLRRELLIFACAFMNFAVVLWLA
                     IYWLMGLPFSTNIPLAYQLVSLGSLVYYLRSQNFRAFRFIQLALFLFVPFIMQWSIGS
                     SVTASGVMLWALLAPIGALVISGWRDSVPWFIAYIVMTVVSGFFDYYLGSGGAGGVPM
                     KTIGFFFALNFAAMSSIMYFLVRYFVVEMDKIRMQLDRQHQLLAEEQQKSERVLFNVL
                     PSSIARRLKDHDGLIADGYADVTVMFADLVNFTQLTESLSPEQMVALLNTIFSGFDEL
                     CEKHGVEKIKTIGDAYMVVGGLNREHVNYTSDIADLALEMREYVVNDPELARYRLGIH
                     TGIATGPVVAGVIGTKRFIYDLWGDTVNIASRLTDEAVEGVIQVDRTTYNRIRQDYAF
                     EPPATIFVKGKGEMVMYRLNARLDAAGSQS"
     gene            7880..8179
                     /locus_tag="VFW59_00655"
     CDS             7880..8179
                     /locus_tag="VFW59_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5362756.1"
                     /translation="MEQLLANRSVSITELKRSPSAIIEQAGDEAVAVLNHNRPAAYLI
                     PAAMYMRQMAALETAGLREAIASGRRDSRPAIPAAQMFAEMDAAIDQVVTEQASA"
     gene            8197..8508
                     /locus_tag="VFW59_00660"
     CDS             8197..8508
                     /locus_tag="VFW59_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5362757.1"
                     /translation="MEFAEWARKDLLGIARYIARDNPQRARSFVSELRQQCALLAEQP
                     GLGVARPDLAADLRQLPYERYVIFFSEIDSGVLIERVLHSARNLTQQFDAPFEPSSDE
                     H"
     gene            8613..9149
                     /locus_tag="VFW59_00665"
     CDS             8613..9149
                     /locus_tag="VFW59_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5362758.1"
                     /translation="MTNYRRSNVAGACYFFTVNLADRQQSLLTDNIALLRNAFEYTRE
                     RHPFVIDAIVILPDHLHAIWTLPEGDRDFALRWRLIKTVFSRGLPLGERRSDSRQEKG
                     ERGIWQRRYWEHLIRDDADYARHVDYIHINPIKHGLALRVADWPHSSFHRFVQAGVLP
                     EDWAGDAGAVWECGERRG"
BASE COUNT         1768 a         2741 c         3136 g         1916 t
ORIGIN      
        1 atccccggcg gcctgcccgg cacgcccgag gaagacggcg accgctacat cgaaatctgg
       61 aacaacgtgt tcatgcagtt caaccgcgac gaggcggggg tgatgcatcc gttgcccaag
      121 ccatcggtgg acaccggcat ggggctggag cgcatctcgg cggtgctgca gcatgtgcat
      181 gcgaactacg agatcgacct gttccaggcg ctgattgcgg ctgcggcacg cgagacgaac
      241 tgcagcgacc tggattctcc atctctgaaa gtgctggccg accatatccg cgcctgctcc
      301 ttcctgatcg ccgacggcgt aatccccggc aacgaagggc gcggctacgt gctgcgccgc
      361 atcatccgcc gcgcgatccg ccacggctac aagctgggtg cgcgcgatgc gttcttctac
      421 cggatggtgc ccgatctggt cgaacagatg ggtgcggcct atcccgaact ggtggccgag
      481 caggtgcgcg tgaagggcat cctcaaacag gaagaggagc gcttctttgc gaccatcgag
      541 cacggcatgg cgatcctcga agccgatctg gccgggatgg atcaggcggg cgtgaaggtg
      601 ttcaacggcg agaccgcgtt caagctgcac gacacttacg gcttcccgct ggacctgact
      661 gccgacattt gccgcgagcg cggtgtgacg gtggacgatg cggcgttcaa cgcggcgatg
      721 gcgaaccaga agcagatggc gcgctcggcg ggcaagttca agatggcggc caacctcgaa
      781 tacgaaggtc cggccacgac cttccacggt tatgacacgc tggaacacaa gggcaacgtg
      841 ctggccctgt acaaggacgg cgtgtcggtg aatcagcttg ccgagggcga gatgggcgtg
      901 gtggtgctgg acgacacccc gttctatgcc gagtccggcg gacaggtggg cgactgcggc
      961 gagctgcgca gcgtgcatgg catcttcgcg gtcgaggaca cgcagaagat ccaggcttcg
     1021 gtgttcggtc atcacggcac ggtcacgacc ggcacgctga cggtgggcaa cggcgtcacc
     1081 gcaaaggtgg atgtcgctgc gcgtagtcgc accatgcgca accactctgc cacgcatctg
     1141 atgcacaagg ccttgcgcga agtgctgggc acgcatgtgc agcagaaggg atcgcaggtc
     1201 gatgccgaca agacgcgatt cgacttcgtg catacccagc cgatcagcga cgaggatatc
     1261 cgcaaggtgg aagccatcgt caacgccgag attttcgcga atgtggcctg tcaggcgcgc
     1321 gagatggcca tcgaggatgc gcagaaaacc ggcgcgatga tgctgttcgg cgagaagtat
     1381 ggcgatgtgg tgcgcgtgct gaatatcggt tcgtcgatcg agttgtgcgg cggtacccat
     1441 gtgcagcgca ccggcgacat cggtctgttc aagatcgtcg ccgagagcgg tgtggcggcc
     1501 ggtgtgcgcc gcgtcgaagc ggtcacgggc gagggcgcgt tggcgctggt gcagcagcag
     1561 gagcagttgt tgcagcaagt ggcggacgcg gtgaaatctc agcctcagga agccgcatcg
     1621 cgcatcgcgc agatactcga caacgtgaag tcgctggaga aggaactggc cgcgctgaag
     1681 tcgaaactgg cttccgctca gggcgacgag ttggtcgcgc aggctcagga catcaacggc
     1741 gtgaaggtgc tggccgccaa gctggacggc gcggatgcgg cagccttgcg cgagacgatg
     1801 gacaagctga aggacaagct caagtcggcg gccatcgtgc tggcttcggt gacggacggc
     1861 aaggtcgcgc tgatcgcggg cgtgacgccc gatctgacgg cgaaggtgaa agccggcgag
     1921 ctggtgaatg tggtcgcgca gcgggtcggc ggcaagggcg gcggacgccc ggacatggcg
     1981 atggcgggcg gcacgcagcc cgagcatctc ggtcaggcgt tgtcggacgt gtacgcttgg
     2041 gtagcaaccc gaacccagga gtgaatggtg agtactgccg ctcgtgagtt attgccggtt
     2101 cgcaacgtgc tcttgatcga ggatgacccg tccctcacgg agtgggtaaa acaggccgct
     2161 gccgatttcg atacgcgtat cgagctgacc gtgctgccag tgggcgtggc cgtgctggag
     2221 tggctgggcg gcgtgaccag aaaacgcttg ccgcacatca ttctgctgga cctgaagctg
     2281 ccgaagctgg acggtctggc cgtgttgcgc acgttgcgct cgaacgtcgc cacccgcgac
     2341 accccgatcg tggtgttctc cgaggaatat attcaggccg atgtggtgtt gggctatcag
     2401 gtcggtgcga acagcttcat cgccaaaccg gcgactcagg aagcgtgcgc ggagtgtctg
     2461 cgcgagcagt tgtcctactg gctgcagccg cgtcagcggg aactggcgct cggcgcacag
     2521 tagctgcaga ttcccgatgg ctggagccgt cgaggcggcg gtcagaacgg cagctgcagc
     2581 gcgggattcg ccttgagcaa cacccgccgg aagtcgtcct gaatgcgctg cagcgccgcc
     2641 tcgtcgtcgg cctcgaaacg cagcacgatc accggcgtgg tgttggaggc gcgcatcagg
     2701 ccgaagccgt cttcgtattc cacccgcagg ccgtcgatgg cgatgacctc tccggcatcg
     2761 gtgaacctgg cttcccgtgc cagccgttcg atcagcgcgt ggctctcccc ctcctgcagc
     2821 ttgatgtgca gttcgggcgt gctgcaggca tcgggcagcg cgttcagcgt ggcgctggca
     2881 tcggcggtgc ggctgaggat ctccagcagg cgcgcaccgg catacaggcc gtcgtcgaag
     2941 ccgtaccagc gttccttgaa gaatacatga ccgctcatct cgcccgccag cagcgcaccg
     3001 gtctcgcgca tcttggcctt gaccagcgaa tggccggtct tccatagcag cggcttgccg
     3061 ccatgctcgc gtatccaggc aaacagattg cccgtggatt tgatgtcgaa gatgatctcc
     3121 gcgcccggat tgcgttccag tacgtcggcc gcgaacagca tcagctggcg atccgggtag
     3181 atgatcctgc cgtcccgggc gactacgccc agacggtcgc cgtcgccgtc gaaggccagt
     3241 cccagttcgc tgccgttcgg gtgcccggac aagagatttg tgtcgtcgcg cagcgcttcg
     3301 atcaggtctg cgaggttgcg cgggtcggag gggtcgggat gatggttggg gaagttgccg
     3361 tccacctcgc agaacagttc ggtcacgtcg caacccagcg cgcgatacag tcttgcggcg
     3421 aaatcgcccg ccacgccgtt gccgcagtcc acgctgatct tcatcggacg cgcgagtctg
     3481 atgtcgctcg cgatgcgggc cagatattcc ggtgcgatgt cgtattgcga atagcggccc
     3541 gaaccctggg cgaggtcgcc ggtttcgatg cggctgcgca gacgctggat ggcctcgccg
     3601 tacagcgtct cgtccgccag caccatcttc aggccgttgt agtcgggcgg gttgtggctg
     3661 ccggtgatca tcaccgcgca gccggttcca agctggtagg cggcgaaata caccatcggt
     3721 gtggcgaccc tgccgacgtc gatcacgtcg atgccgcttt tccggatgcc gcgcgccaag
     3781 gctagtgcaa actcgggccc ggacagtcgc ccgtcgcggc cgatgacaat ggatttctgt
     3841 ttgcgtgcga tcgcctcgga gccgatcgcc tggccgatct gctcgacggt ttcggcggtc
     3901 agcgtgcggc ctacgatgcc gcgaatgtcg taggccttga atatttcggt ggagggaagt
     3961 tcgcgcagcg actcgtttgc cccctctccc gcaagcggga gagggttggg gagagggggc
     4021 agggaagtca tgccatcact ccgtcatcag cggctgaatt tcttgccaca cccggtccgg
     4081 ggtgagctgc atcatgcaat tgaaatgtcc cagcgggcac tcgcgtttga agcaggggct
     4141 gcagggcagg tcgagctgga cgacgcgtgc gcggccggac agcggcgggg tgaattgcgg
     4201 gctgctggag ccgaagaggg cgatcatcgg ccgctccagt gcggcagcca tatgcatcag
     4261 gccggaatcg ttgctgacca cgagcttggc gcaggacagc agcgcgatcg cgtcgcccag
     4321 atcggtgcgt ccgcacaggt tgtggcaggc gttgccgctc agttcgtcga tctcgtccgc
     4381 cacggcacgg tccttgttcg aaccgaatag ccagattgcg tagccttgcg tgctcaggcg
     4441 tttggccagt tcggcatagt aggcgggcgg ccagcgcttg gcggggccgt attcggcgcc
     4501 ggggcagaac gccgcgacgg ggcgatccag tgtgagcccg agtttattca gagcggcatc
     4561 gcgctgcgca gcggaaacct tcagcgccgg attggataag gggcgcggaa tctggccctg
     4621 cttgtcttcg gccagttgcg cgaagcgttc caccatcagc ggcaagcgtt gtttgtcgag
     4681 cttgcgcgca tcgttgagca ggccgtaacg catctcgccg acgaagccgg tgcgcaacgg
     4741 aatgcccgcg aagaacggca ccagtgcgga tttcagggaa ttcggggtga cgatggcctg
     4801 atcgtaacgc gcgttgcgca attgccggcc cagccggtaa cggctgccca attgcagcgc
     4861 gccgtgcgga aacggattga cgatgacttc gctgacctcg ggcatctggc gcagcaactt
     4921 ctcggtccat ggcggagcga gtatgtcgat gcggcagtcc ggatggcgtt gcatgaggcg
     4981 ttgcagcatc ggctgcatca gcatgcagtc gcccacccag ctgggtgcga tgaccaggat
     5041 cttatacatt cgattcctta gaacccctct cccgcaggcg ggaaaggggc tgggggaagg
     5101 ggcagggaat caatggccgc cgcgcggcaa ttcgcccttg aacttgtaca gcgtgccgca
     5161 atacgggcag cgcgcttcgc cgccgttctt gagcgggatg gataccctgg ggtgggcatc
     5221 ccagtggctc atgttcggca tcgggcaggt caacggcaga tcatgcgcgg tcacttcgat
     5281 gtaacgctgg gtgatggtct gattgctcat gatgactccc ttagacgtag ctcagccagt
     5341 cggcgtgttg cggatgcttg cccgcgacac agtcgaaata ggccttctgc aggcgctcgg
     5401 tgaccgggcc tcggcagccc gcgccgatgg tgcgctgatc cagttcgcgg atcggcgtga
     5461 cttcggcggc ggtgccggtg aagaaggctt cttcggcgca gtagatctcg tcgcgggtga
     5521 tgcgcttctc gatcacctcg atgcccatct cctttgccag cgtgatgatg gagtcgcgcg
     5581 tgatgccttc caggcaggag gtcaggtcag gggtgtacag cttgcccttc ttgacgatga
     5641 acaggttctc gcccgcgccc tcggccacgt agccgtccac gtccagcagc agcgcctcgt
     5701 cgtagttgtc gttggcaact tcctgatgcg cgaggatgga gttggcgtag gtcgtcaccg
     5761 acttggcgcg gcacatgttc acgttgacgt gatggcgggt aaagctggat gtcttgacgc
     5821 ggatgccttt ctgcatgcct tccgcgccca ggtaggcgcc ccacggccat gcggcgaccg
     5881 caacatgggt ggacagcgtg gtggcggcga tgcccatcgc ctcggagccg tagaaggcga
     5941 tcgggcggat gtagcaggac ttcagcttgt tgagacgaac cacttcgcgg tgcgcctcca
     6001 tgacggtttc cttgtcgtag ggcatcttca tctggaagat gtgcgcggag ttgaacaagc
     6061 ggtcggtgtg ctcctgcaga cggaagatcg ccgtgccttt ctcggcctcg taggcgcgca
     6121 gcccctcgaa tacgcccatg ccgtagtgca gggtatgggt cagtacatgg gtggtggcat
     6181 cgcgccatgg cacgatcttg ccgtcgtacc agataaagcc gtcgcggtca gacatcgaca
     6241 taatggggtt ccttaatgat gagcgattgc aaagtcagga ttctagcgtt tccgcgcgga
     6301 tttatgaagc gtcagtgtta aacccttgcg cgagtccggg tgttttctgc aaagccattt
     6361 cgggtatgat gaaagcgtct gtaccgtttg gaagtgcagg ctctcttacc aacagtgggc
     6421 agcgatggcc gaaaagttga cacaatctcc cgatatcacc gcaccggcgg gcaactcgca
     6481 agaagataaa ttgcgccggg agttgctgat cttcgcctgt gccttcatga attttgcggt
     6541 ggtgttgtgg ctggccatct actggctgat gggcttgccg ttctcgacca atatcccgct
     6601 cgcctatcaa ctggtgtcgc tcggttcgct ggtgtattac ctcagaagcc agaatttcag
     6661 ggccttccgt ttcattcagc tggcgctgtt cctgttcgtc cccttcatca tgcaatggag
     6721 catcggcagt tcggtcaccg ccagcggcgt gatgctctgg gccttgctgg ccccgatcgg
     6781 tgcgctggtg atctccggct ggcgcgattc ggtgccctgg ttcatcgcct acatcgtgat
     6841 gacggtggtg tcgggtttct tcgactatta cctcggctcc ggcggcgcgg gcggggtgcc
     6901 gatgaagacc atcggcttct tcttcgcact caatttcgcc gcgatgtctt ccatcatgta
     6961 ttttctggtg cgctatttcg tcgtggaaat ggacaagatc aggatgcagc tcgatcggca
     7021 gcaccagctg ctggccgaag agcagcagaa atccgaacgc gtgttgttca acgtgctgcc
     7081 cagcagtatc gcgcgccgcc ttaaggatca tgacgggctg atagccgacg gctatgccga
     7141 tgtgacggtg atgttcgccg acctggtgaa tttcacgcag ctgacggaat cgctctcgcc
     7201 cgaacagatg gtcgcgctgc tcaacacgat tttttccggc ttcgacgagc tgtgcgagaa
     7261 acacggcgtg gagaagatca agaccatagg cgacgcctac atggtggtgg gcggactgaa
     7321 tcgcgagcat gtcaattaca ccagcgacat cgccgacctg gcgctggaga tgcgcgaata
     7381 cgtggtcaac gatccggagc tggcccgcta ccggctgggc atccacaccg gtatcgccac
     7441 cggcccggtg gtcgccgggg tgatcggcac caagcgcttc atctacgacc tgtggggcga
     7501 cacggtgaac atcgccagcc gcctgaccga cgaggcggtg gagggcgtga tccaggtgga
     7561 ccgaaccacc tacaaccgca tccggcaaga ctacgccttc gagccgcccg ccacgatctt
     7621 cgtcaagggc aagggcgaga tggtgatgta ccggctgaat gcgcgtcttg acgctgccgg
     7681 ttcgcaatcc tgattcacgg gggcgtcgtc agaggcgctt tcccgtaggc ggacgcaata
     7741 gacaaactca gggccgcgcc cgcttcaccc ctaaactggc agggctgaga cctttgcatc
     7801 gggacacaat ctcattgacc atcgcagaat gaatggtcac aattcggcat cttatttggt
     7861 gcttttttga gttaaagaca tggagcagtt attggcgaac cgatcggtta gcatcaccga
     7921 gcttaagcgc agccccagcg ccatcattga gcaggcgggc gatgaagcgg tcgccgtgct
     7981 gaaccataat cgcccggcag cgtatttgat ccctgctgct atgtatatgc gtcagatggc
     8041 tgcgctggaa acggcgggtt tgcgtgaagc gattgcctct ggccgacgcg actcgcgtcc
     8101 ggccataccc gcagcgcaaa tgtttgccga gatggatgcc gcaattgatc aggtggtcac
     8161 tgaacaagcc agcgcgtaat gtccaagcca gcacaattgg agttcgccga atgggcgcgc
     8221 aaagatttgt tgggaatcgc ccgttacatt gctcgcgaca atccgcaacg cgcccgtagt
     8281 tttgtgagcg aactgcgcca gcaatgcgct ttgttggcag agcaacccgg gctgggtgtc
     8341 gcgaggccag acttggccgc agacctgcgt caacttccct atgagcggta tgtgatcttc
     8401 ttttctgaaa tcgattccgg tgtcctgata gagcgggtgc tgcacagcgc gcgcaatctt
     8461 actcagcagt tcgatgcgcc atttgagcct tcgtctgacg aacattagac taaggctcag
     8521 gttagtcgga accaaagtca aaaccggcaa gcgtggaggt gcagagttgt aggatgggtt
     8581 gagcgtagcg atacccatcg ttatcatgcc tcatgaccaa ctaccgccgc agcaacgtcg
     8641 ccggagcctg ttacttcttt acggtgaatc tggcagaccg tcaacaatcc ctgctgaccg
     8701 acaacatcgc attgctgcgc aacgctttcg aatatacccg cgagcgtcat cccttcgtca
     8761 ttgatgccat tgtgatattg ccggatcacc tgcacgcgat ctggacgctg cccgaaggcg
     8821 acagagattt tgcattgcgc tggagactga tcaagacggt gttctcacgc ggcttgcccc
     8881 tcggtgaacg tcggtcggat agtcggcagg aaaagggcga acgcggtata tggcagaggc
     8941 gatattggga gcatctcatt cgcgacgatg ccgattatgc gcgccatgtc gattacatcc
     9001 acatcaatcc gatcaagcac ggcttagcgt tacgagtagc ggactggccg cattcgtcgt
     9061 ttcatcgctt cgtacaggcg ggtgtattgc cggaggattg ggcgggtgat gcgggagctg
     9121 tctgggaatg cggcgagcgg agggggtgat gatgggtatc gctgcgctca acccatccta
     9181 cgcgagctgg gaatgcggcg agcggagggg gtgatgatgg gtagctctgc atagccatct
     9241 gactaggctg tcaaaatacg tcagccaagt cattggttat cgctgcgctc aacccatcct
     9301 acgcgggctg aaatgggttt ttctacagcg tcaacgtaaa gttgaggggc gctgcgcttt
     9361 tgcgcagtcc ctctcgactg ccgggttaga ccacactttg cggctcatgg catgagtaca
     9421 attgggccgc agtttacttc gtggttcacg gtgtctgctg gcagtacatg ttgaaaaagc
     9481 gtcaggaagg tttcgccttc cattgagcgc ggcgatacaa gattttttat caatggcacg
     9541 agtttgacgg cggcgttcac a
//
LOCUS       DASUIV010000018         4870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_934554, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000018 DASUIV010000000
VERSION     DASUIV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934554"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /gene="glnA"
                     /locus_tag="VFW59_00670"
     CDS             complement(<1..1027)
                     /gene="glnA"
                     /locus_tag="VFW59_00670"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX5362759.1"
                     /translation="MTANDVLKMIKDHDVKFVDLRFTDTRGKEQHVGVPAKYVGLDKF
                     EEGHAFDGSSIAGWKGIQASDMLLMPDPTTAYLDPFMDENTLVITCDVIEPTDGKGYD
                     RDPRSIAKRAEAYLKSTGIGDTAFFGPEPEFFIFDSVTWTVDMSGCTLKINSEEAAWA
                     TGEKFEGGNTGHRPMVKGGYFPVPPVDSLNDIRAAMCLALEDIGIEVEVHHHEVATAG
                     QCEIGTKFSTLVKRADQTQALKYIVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQSIW
                     KDGKNTFAGNGYAGLSETALYYIGGIIKHAKSLNAICNPGTNSYKRLVPHYEAPVKMA
                     YSAKNRSA"
     gene            complement(1210..2400)
                     /locus_tag="VFW59_00675"
     CDS             complement(1210..2400)
                     /locus_tag="VFW59_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5362760.1"
                     /translation="MKNHNHQYAIPDDGADFEPMNPERFAAAQKSTWVSIIINLVLTF
                     LQVLGGLFAHSQALVADGLHSLSDLLSDVLVLYANRHGNRHADANHPYGHARVETAAT
                     LILGTFLAVLGVVLLVAAAMRLQHPEALQAVNPWALGIGVVALIAKEGMFRYMLAVAK
                     RVRSQMLVANAWHARSDAASSLVVIVGVLGNLLGYTFFDLLAAAVVGVMIAHMGGKLA
                     LEAMAELIDTGLDAEEVEAIRNTLRNSPGVCGLHELRTRKMADNALVDAHIMVDPKIS
                     VSEGHYIAETARQAVLSAHHVLDVMVHIDPEDDRKVKPNAHLPDRAGLLDHLAAQLGD
                     PSIKDCRVVFHYLDGKVDAEIYLSAQQQSVEQVDALQAACNEMARGDEHFRSIQIHRS
                     SALN"
     gene            2484..2936
                     /locus_tag="VFW59_00680"
     CDS             2484..2936
                     /locus_tag="VFW59_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5362761.1"
                     /translation="MGKITAILQTAQQRAKELNLPYEGALLPKEAFEILQSAPGARLV
                     DVRSRAECDWVGRVPGAVETEWASYPGMVRNPDFVAQLEQSVDKESLVLFLCRTGQRS
                     HAAAALATEIGFADCYNILEGFEGDKNTGGQRRSTNGWCAAGLPWQQG"
     gene            complement(2965..3729)
                     /locus_tag="VFW59_00685"
     CDS             complement(2965..3729)
                     /locus_tag="VFW59_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5362762.1"
                     /translation="MRIITINLNGIRSATSKGFYEWLAQQDADVVCLQELKAQAADMT
                     EQMLNPLGYCGHFHYAEKKGYSGVGIYSRHKPERVIEGLGIAEFDSEGRYIEAQYGKL
                     SVVSLYLPSGSSGEERQAVKFNFMAAFMPHLRELRASGREVIVCGDWNIAHKEIDLKN
                     WRGNKKNSGFLPEERAWLTELFDEVGFVDVFRRVHPELEAYTWWSNRGQAWAKDVGWR
                     IDYQIATPGTAERAESASVYKAQRFSDHAPLTIDYS"
     gene            3799..4446
                     /gene="pyrE"
                     /locus_tag="VFW59_00690"
     CDS             3799..4446
                     /gene="pyrE"
                     /locus_tag="VFW59_00690"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5362763.1"
                     /translation="MSNFSQDFIRFAVQQNVLCFGEFQTKAGRMSPYFFNAGLFNDGT
                     SLRNLSQFYAQAILASEIPFDMLFGPAYKGIPLAAGTAIALSEQGRNVPYCFNRKEAK
                     DHGEGGTMVGAKLQGRVLIIDDVISAGTSVRESIELIRAAGAEPCGVVIALDRMERGQ
                     GELSAVQEVQRNYSIPVVHIAGVDDLLSYLQGEPEMVQNLSAVKSYRAQYGVKND"
     gene            4427..>4870
                     /locus_tag="VFW59_00695"
     CDS             4427..>4870
                     /locus_tag="VFW59_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5362764.1"
                     /translation="MELKMTDKTTIHSTQLSKNDNQVAGYKSKLLITLIAGLAFSLPV
                     NAKMYKWVDDKGVTHVGDTIPPEYANKDRTELNKSGRAIKKDEVLNAEERRAKDQLDT
                     QKRAADEAVLESRRHDKALLSTYSNSAEIDLARSRNMQQIDARVSG"
BASE COUNT         1091 a         1445 c         1331 g         1003 t
ORIGIN      
        1 aagcggaacg gttcttcgcc gagtaggcca tcttcaccgg cgcttcgtag tgaggaacca
       61 gacgcttgta ggagttggtg ccgggattgc agatcgcgtt cagcgacttg gcatgcttga
      121 tgataccgcc gatgtagtac agagcggtct cggacaggcc tgcgtagccg ttgcctgcga
      181 aggtgttctt gccgtctttc cagatcgact ggtgcacgtg catgccggaa ccgttgtcgc
      241 caacgatagg cttcggcatg aaagtggcgg tcttgccgta ctggtgcgca acgttatgca
      301 cgatgtactt caatgcctga gtctggtcgg cgcgcttcac cagcgtgctg aacttggtgc
      361 cgatttcgca ctggccggcg gtcgcaactt cgtggtgatg cacttcgact tcgataccga
      421 tgtcttccag cgccagacac atcgcggcgc ggatgtcgtt caggctgtcg accgggggaa
      481 ccgggaagta gccgcccttg accattggac ggtggccggt gttgccgcct tcaaacttct
      541 cgccagtggc ccatgccgct tcttcggagt tgatcttcag cgtgcagccc gacatatcaa
      601 cggtccaggt cacggagtcg aagatgaaga attcaggctc aggaccgaag aatgcagtgt
      661 cgccgatgcc ggtcgacttc agataggctt cagcgcgctt cgcgatggag cgcggatcgc
      721 ggtcgtagcc cttgccgtcg gttggctcga tcacgtcaca ggtgatgacc agtgtgtttt
      781 cgtccatgaa aggatcgaga tatgccgtcg taggatccgg catcagcaac atatcggaag
      841 cctggatacc cttccagcca gcaatggagg agccgtcgaa agcatggcct tcttcgaact
      901 tgtccaggcc cacatacttg gcaggcacgc ctacatgctg ctctttacca cgggtatcgg
      961 tgaaacggag atccacaaac ttgacgtcgt ggtctttaat catcttcaac acatcatttg
     1021 ccgtcatcga cataactctc tccaggatca aataaataca gcgaacaaaa ttaatgaaac
     1081 cagtaatttc agaatgcaga aagcgtgcca actactatcc gcatattagg ccagcattgt
     1141 gccataagcc cgcaacacgg cacagaaaat atttgcacca aaatcgggcg caaattgccc
     1201 gcaattgcac taatttagtg cggaactgcg gtgtatctga atggaacgaa aatgttcgtc
     1261 cccccgcgcc atttcattgc atgcggcctg caatgcatcg acctgctcga cggactgctg
     1321 ttgagccgaa agatagattt ccgcatcgac cttgccatcc aggtaatgga acaccacccg
     1381 acaatccttg atcgaggggt cgcccaattg agccgcgaga tgatccagca gaccggcgcg
     1441 atcgggcaga tgggcattcg gcttgacctt gcggtcatcc tccggatcga tatgcaccat
     1501 cacgtccagc acatggtggg ccgacagcac agcctggcgc gccgtttcgg cgatgtaatg
     1561 cccctcggag acgctgatct tgggatcgac catgatgtgc gcatccacca gggcgttatc
     1621 cgccatcttg cgcgtacgca attcgtgcaa cccgcagacg ccgggagaat tgcgcaatgt
     1681 attgcggatt gcctcgacct cttccgcatc cagcccggtg tcgatcagct cggccatcgc
     1741 ctccagcgcc agcttgccgc ccatgtgcgc aatcatcacc cccactaccg ctgccgccag
     1801 caaatcgaag aaggtataac caagcaggtt gcccagcacc ccgacgatga ccaccagcga
     1861 cgaagccgca tcggaacgcg cgtgccaggc gttggccacc agcatctgcg aacgcacccg
     1921 cttggctacc gccagcatgt aacggaacat gccttctttc gcaatcaaag cgacgacccc
     1981 gatgccgagc gcccacgggt tcaccgcctg caacgcctcg ggatgctgca ggcgcatcgc
     2041 tgccgccacc agcagcacca caccaagcac cgccaggaat gtccccagaa tgagcgtcgc
     2101 ggcagtttct acccgcgcat gcccataagg atgattggca tcggcatggc gattcccgtg
     2161 ccggttcgca tacagcacca gcacatcgga caacaagtca gacagcgaat gcagtccgtc
     2221 ggcaaccagg gcctgcgaat gcgcgaacag ccctcccaaa acctgaagga atgtcagcac
     2281 caggttgatg atgatgctca cccaggtgct tttttgtgcg gcggcgaatc gctccggatt
     2341 catgggttca aaatccgcgc cgtcatccgg gatcgcgtat tgatggttat gatttttcat
     2401 atcggttgcg ctagtatgtg tccacgtcgt gcagcataac atccgcacca attctgccaa
     2461 acctcgccat agaaagaacc atcatgggaa aaatcactgc gatcctgcaa accgcccaac
     2521 aacgagccaa ggaacttaat ctgccttacg aaggtgcgct gctgcctaaa gaagcgtttg
     2581 agatattgca atccgccccc ggcgcccggc tggtcgatgt ccgttcccgt gccgaatgcg
     2641 attgggtagg gcgagtgccc ggtgcggtcg agaccgagtg ggccagctac cctggcatgg
     2701 tgcgcaatcc agacttcgtg gcccagctgg aacagtcggt cgacaaagag tcgctggtgc
     2761 tctttctgtg ccgtaccggg caacgttccc atgcagcagc agccttggcg accgagatcg
     2821 gctttgccga ttgctacaat attctggaag gctttgaagg cgacaaaaac accggcggcc
     2881 agcgccgcag caccaatggc tggtgcgctg cagggctgcc ttggcagcag ggctaaccct
     2941 gcaagggcac ggcgcgccgc gcccctacga gtaatcgatc gttagcggcg cgtgatcaga
     3001 gaagcgctgc gccttgtaga cactggccga ctcggcgcgc tctgcggtac ccggcgtagc
     3061 aatctggtaa tcgatacgcc aacccacatc cttggcccat gcctgccccc ggtttgacca
     3121 ccaggtatag gcctcgagtt cgggatgcac ccggcggaac acgtcgacga aaccgacttc
     3181 atcgaacagt tcggtcagcc aggcgcgctc ctcgggcaaa aaacccgagt tcttcttgtt
     3241 gccgcgccag ttcttcaggt cgatttcttt atgcgcgatg ttccaatcgc cacagacgat
     3301 tacttcacgc ccactggcgc gcagctcgcg cagatgcggc atgaatgccg ccatgaagtt
     3361 gaatttcacc gcctgacgct cttcaccgct ggagccggat ggcagataca acgacacaac
     3421 gctcaattta ccgtactgtg cctcgatgta gcgcccctcg ctatcgaact cggcgatacc
     3481 cagcccctct atcacccgct cgggcttatg ccgggaatag attcccaccc cgctgtagcc
     3541 tttcttttct gcatagtgaa aatggccgca gtaacccaac gggttcaaca tctgttcggt
     3601 catgtccgcc gcctgggctt tgagttcctg caagcagacc acgtctgcat cctgttgcgc
     3661 cagccattcg taaaagcctt tgctggtcgc cgaacgaatc ccgttcaggt tgatggtaat
     3721 gatacgcatg aaagttttcg ggtgttgtcg ggcgcgacat tataatgacg tccgtcttct
     3781 ctacctacgg tgttccacat gtccaatttc agccaggatt tcatccgctt cgccgtacag
     3841 caaaacgtgc tgtgcttcgg cgaattccag accaaggcgg ggcgtatgtc gccatacttt
     3901 ttcaatgcgg gcctgtttaa cgatggcaca tcactgcgca atctgtcgca gttctatgcg
     3961 caggccatcc tcgcctcaga gattcccttc gacatgctgt tcggcccggc ctataaaggc
     4021 atccctctgg cggcaggaac cgcgatcgcc ctgtccgaac agggacgcaa tgtaccttat
     4081 tgctttaacc gcaaagaagc caaggatcac ggcgaaggcg gcacgatggt aggggcgaaa
     4141 ttgcagggcc gcgtgctgat catcgacgac gtgatctcgg caggaacctc agtacgagaa
     4201 tccatcgaat tgattcgcgc agcaggtgcc gagccctgtg gcgtggtcat cgcactggat
     4261 cgtatggagc gcggtcaagg cgagctttct gcggtgcaag aagttcagcg caactacagc
     4321 atcccggtag tgcacattgc cggcgtggat gacctgctca gctatttgca aggagagccc
     4381 gagatggtgc agaatttgtc ggcggtgaaa tcttaccgtg cccaatatgg agttaaaaat
     4441 gactgataaa actactatcc attccactca gttgtcaaaa aacgacaacc aagtggctgg
     4501 ttataaatca aaactgctta tcactctcat tgccgggttg gcattcagcc tgccggtcaa
     4561 cgccaagatg tataaatggg tggacgacaa gggcgtcacc catgtgggag acacgattcc
     4621 gccggaatat gccaacaagg atcgcaccga attgaacaag tcaggtcgcg ccatcaagaa
     4681 ggacgaggtg ctcaacgccg aagaacgccg ggcaaaggat cagctcgaca ctcaaaaacg
     4741 ggcagccgat gaagccgtat tggagtcaag gcgccacgac aaagcgctgc tgagcacata
     4801 cagcaattcg gccgagatcg acttggcgcg cagccgcaac atgcaacaga tcgatgcgcg
     4861 cgtcagcggc
//
LOCUS       DASUIV010000019         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_562672, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000019 DASUIV010000000
VERSION     DASUIV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562672"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1392
                     /locus_tag="VFW59_00700"
     CDS             1..1392
                     /locus_tag="VFW59_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5362765.1"
                     /translation="MSLMKKVGLVGLGLVAGVSISLHFAAIADKETLATPLPIEELRA
                     FSEVFGRIKSDYVEPVSDKKLINEAINGMLSGLDPHSAYLDPEAFKELQVGTQGEFGG
                     LGIEVGMEDGLVKVVSPIEDTPAFQAGIKSGDLIVKLDDTLVKGMTLNDAVKRMRGKP
                     GSKIVLTIIRKEQPKPMTFTLVRAVIKVQSVKSKLVDPGYGFVRVTQFQEHTGENLAK
                     ALEALVKQNKGQLKGLVLDLRNDPGGLLTGAVGVSAAFLPKDALVVYTEGRTEDAKMR
                     LTATPADYLRGNGKDDYLADLPASIKTVPMVVLVNGGSASASEIVAGALQDHKRAVIM
                     GTQTFGKGSVQTVLPLGNNTAIKLTTARYYTPGGRSIQAKGIVPDILVEDPSNLEQDN
                     AFRLREADLDRHLTNDKQPETNAPASGKAPAAPGKNGDAAKTAPAEFGAKNDYQLNQA
                     LNLLKGMQILHGK"
     gene            1403..1810
                     /locus_tag="VFW59_00705"
     CDS             1403..1810
                     /locus_tag="VFW59_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362766.1"
                     /translation="MINPFDLEKQHDIVFSAEPPGQLERALPILYGLPNCKVEIVGDH
                     PHTLRVTYNLHDYTLEGLEKALTEEGFQLDHSMLHSIGRKVIYYCEDTACHNMDIPVH
                     QTKQNERDVFIKSYDSESHGDHDDIPPELRDYK"
     gene            1832..2044
                     /locus_tag="VFW59_00710"
     CDS             1832..2044
                     /locus_tag="VFW59_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362767.1"
                     /translation="MSETSAPSLPNCLECRHYYVTWDVGFPYGCRAMDFKSRRRPQLD
                     VLASSGAPCLRFEPRLPPGATRHNGS"
     gene            2204..2719
                     /locus_tag="VFW59_00715"
     CDS             2204..2719
                     /locus_tag="VFW59_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HEX5362768.1"
                     /translation="MGLSWNKQLSIANTIVDSEHKHLISLTNQVERAMKTAMELRNDT
                     PLQQAFEQLENELQHHFRNEEKIAHAINFPFDQHLQAQHHMLMELRYLRAELLTKDCI
                     WTDAALQHFAQFLQEWMIEHITQADMPMKSTLQGYDYNFWPDWDDKSLAQPHIQRAQE
                     PSRSPLACAAS"
     gene            2914..>3388
                     /locus_tag="VFW59_00720"
     CDS             2914..>3388
                     /locus_tag="VFW59_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5362769.1"
                     /translation="MSDTPEDTLSTPADSGAIAREVKRRRTFAIISHPDAGKTTLTEK
                     LLLFGGAIQMAGTVKARKSGRHATSDWLEIEKQRGISVASSVMQFTYDDCVINLLDTP
                     GHQDFSEDTYRVLTAVDAAVMVVDAAKGVEAQTIKLLEVCRLRNTPIITFINKMDR"
BASE COUNT          756 a         1135 c          964 g          533 t
ORIGIN      
        1 atgagtctca tgaagaaagt cgggttggtc ggtctgggtc tggttgcagg cgtttccatc
       61 agcctgcatt ttgctgccat cgccgacaaa gagacgcttg caacgccgtt gcccatcgaa
      121 gagttgcgcg ccttctccga agtgttcggt cgcatcaaga gcgactatgt ggaacccgtt
      181 tccgacaaga aactgatcaa cgaagccatc aacggcatgc tctccggcct cgatccgcac
      241 tccgcctatc tcgatccgga ggccttcaag gaattgcagg tcggcaccca gggcgaattc
      301 ggcgggctgg gcatcgaagt cggcatggaa gacggcctgg tcaaggtcgt atcgccgatc
      361 gaggacaccc ccgcctttca ggctggcatc aagagcggcg acctgatcgt caaactggac
      421 gacacgctgg tcaagggcat gacgctgaac gacgcggtca aacgcatgcg cggaaaaccc
      481 ggcagcaaga tcgtgctgac gatcatccgc aaggagcagc ccaagccgat gaccttcacg
      541 ctggtacgcg cggtcatcaa ggtgcagagc gtcaaatcca aactggtcga ccccggctac
      601 ggtttcgtcc gcgtcaccca gttccaggaa cacaccggcg agaacctcgc caaggcgctg
      661 gaggccctcg tcaaacagaa caaggggcaa ctcaaggggc tggtgctgga tctgcgcaac
      721 gatccgggcg gcctgctgac cggcgccgtc ggcgtgtccg cagccttcct gccgaaggat
      781 gcgctggtgg tttacaccga gggtcgcacc gaagatgcca agatgcgcct gaccgccaca
      841 ccggccgact acctgcgcgg caacggcaag gacgactatc tggccgacct gcccgcatcg
      901 atcaagaccg tgcctatggt ggtgctggtc aatggcggct ccgcctcggc ttccgagatc
      961 gtcgccggcg cgctgcagga tcacaaacgc gcggtcatca tgggcacgca gaccttcggc
     1021 aagggctcgg tgcagaccgt gctgccgctg ggcaacaaca ccgcgatcaa gctcaccacc
     1081 gcgcgctact acacgccggg cggacgctcc attcaggcca agggcatcgt tccggacatt
     1141 ctggtggaag atccgtccaa tctcgaacag gacaatgcct tccgcctgcg cgaggccgat
     1201 ctggaccgcc acctgaccaa cgacaagcag ccggaaacga atgcgcccgc ctccggcaag
     1261 gcgcccgccg cgcccggcaa gaatggcgat gcggcgaaaa cggccccggc cgaattcggc
     1321 gcaaagaacg actatcagct caatcaggcg ctgaatctgc tgaaaggcat gcagatactg
     1381 cacggaaaat aatacgaccg acatgatcaa cccattcgat ctggaaaaac agcacgacat
     1441 cgtcttcagc gcagagccgc cggggcagct ggaacgcgcc ctgcccatcc tgtacggcct
     1501 gccgaattgc aaggtcgaaa tcgtcggcga tcacccgcac acgctgcggg tcacttacaa
     1561 cctgcacgac tacacgctgg aaggactgga aaaggcactg accgaagaag gcttccagct
     1621 cgatcacagc atgctgcaca gcataggccg caaggtcatt tactattgcg aggacaccgc
     1681 ctgtcacaac atggacatcc cggtgcacca gaccaagcag aacgaacgcg acgtattcat
     1741 caagtcctac gacagtgaat cgcacggcga ccacgacgac atcccgccgg agctgcgcga
     1801 ttacaagtag cctctagccc ccgccggaac aatgagcgag accagcgccc cctccctccc
     1861 caactgcctt gaatgccgcc actactacgt cacgtgggat gtgggctttc cctacggctg
     1921 ccgggcgatg gacttcaaga gcaggcggcg gccgcagctg gatgtgctgg cctcctccgg
     1981 cgccccctgc ctgcgcttcg aaccgcgcct gccgccgggc gcgactcgcc acaacggctc
     2041 ataaccctct catcctccac tgaggccggc tcagccaaac tccccgcccg ccccgccaaa
     2101 tacgagggcg aaatcggctg ccgccgatat tgcgcgcccc tctccttgtg cagaatgcct
     2161 caccacccgg acgcaagccg gatccaaccc tcaggagaac gcaatgggac tgtcatggaa
     2221 caaacaactg agcatcgcca acacgatcgt cgattccgaa cacaagcatc tgatcagcct
     2281 gaccaatcag gttgagcgcg cgatgaagac ggcgatggag ctgaggaacg acaccccgct
     2341 gcagcaggct ttcgagcaac tcgaaaatga actgcagcac cacttccgca acgaagaaaa
     2401 gatcgcgcac gcgatcaact tccccttcga tcagcacctg caggcgcagc atcacatgct
     2461 catggagctg cgttatctgc gcgccgagct gctgaccaag gactgcatct ggacggatgc
     2521 cgccctgcag cattttgccc agttcctgca ggagtggatg atcgaacaca tcacccaggc
     2581 ggatatgccc atgaaatcca cgctgcaagg ctatgactac aacttctggc ccgactggga
     2641 cgacaagtcc ctcgctcagc cgcacatcca gcgcgcccag gagccgagcc gatccccgct
     2701 cgcctgcgcc gcatcctgag cccgccgcgc atcggcacct tcgtgactgg cgcggcagcg
     2761 tcggaagccc gatttctgat accatgcgcg ccggttttaa caagtagcgc agcgcaccca
     2821 tagcctcacc ggccggttcg acaaggtctg cacgttctgc gtgcagacct ttcgccttca
     2881 agggcaccgc gttgcgcgca ggaaaagcaa gccatgagcg atacaccaga agacaccctt
     2941 agcacgcccg ccgacagcgg cgcgatcgca cgcgaagtca aacgccgccg caccttcgcg
     3001 atcatctcgc acccggacgc gggcaagacc acgctcaccg aaaaactgct gctgttcggc
     3061 ggcgcgatcc agatggcggg cacggtcaag gcgcgcaaga gcggccgcca cgcgacctca
     3121 gactggctgg agatcgagaa gcagcgcggc atctcggtgg ccagctcggt gatgcagttc
     3181 acctacgacg attgcgtgat caacctgctc gacacgcccg gtcaccagga tttttccgag
     3241 gacacctatc gcgtgctgac cgcggtggac gccgcggtga tggtggtgga tgcggccaag
     3301 ggcgtggaag cgcagaccat caagctgctc gaagtgtgcc gcctgcgcaa cacgcctatc
     3361 atcaccttca tcaacaagat ggaccgcg
//
LOCUS       DASUIV010000020         6761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_377123, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000020 DASUIV010000000
VERSION     DASUIV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377123"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFW59_00725"
     CDS             <1..256
                     /locus_tag="VFW59_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362770.1"
                     /translation="PEELVAVVNRALRKNLPERHANWDEFIRDLGNAAHKIRMSDYDL
                     DLYRGFSMSTQTVLSSYMSADRDFSRSGFSRSVMPGSFEA"
     gene            complement(260..1540)
                     /locus_tag="VFW59_00730"
     CDS             complement(260..1540)
                     /locus_tag="VFW59_00730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5362771.1"
                     /translation="MTSERTKGHPALIVGAGRGGSALLEMFMEDDLVRVVAMVDTNAE
                     APGLQLAKKYGIPTYTDPVAALQACKDYPDCIVYNLSHDDTIAEQASKVFGDKRVASG
                     PEVKLFWQMVTNLKQVKGELEKSQLQLQSVIHNVMDGIITTDESGAIQGFNPAAENIF
                     GYSQEEITGQNIRILMPESDRSGHDVHVQRYLKSGDSQLLGMRNREVTSVRKNGETFS
                     MEISISEMTLGGQKYFVAIVRDITERKKAEEKIAHLAHYDFLTDLPNRAMFMNTLGHS
                     MLLAKRNGYKVAALFLDLDGFKQVNDTLGHDAGDLLLQGVAKRLQEVIRASDTVARVG
                     GDEFTFVLNGIDSEEDVATIARKLIATLSEPFDLQGRQCHVGGSIGASLFPDSSGDMN
                     GLIKQADEAMYLAKQSGKNTYKFYREVLQARAGQ"
     gene            complement(1666..2919)
                     /locus_tag="VFW59_00735"
     CDS             complement(1666..2919)
                     /locus_tag="VFW59_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5362772.1"
                     /translation="MNTPPHDFHRVCSRTHVGNTGHVTAQFRDFSIASAFQPIFSLSH
                     RSPVGYEALCRASAADGSPVSPLALFGQVKDESDNVLLDRLCRAVHVQNFSVFSDKSW
                     IFLNVDPLVTVLGKSYGSFFKEMLEHSQIPPGQVVIEILEKSIYDESILSAAVNYYKN
                     LGCLVAIDDFGASHSNFDRIWNIQPDIVKFDRSIVVQAESNRAVRKALPNMVSLIQEL
                     GCITLMEGVETEQQALIAMDSNVDLVQGYYFGYPREQLVDAHEQDNTLAQLCDKHVEF
                     SAAARNRHKERMDRPMAEFNALLRQLKSGATDAVPLCGRLLALPDVMRMYFLDEAGYQ
                     IGENIVHESKPKYEDPRYRPLAEATEANWSRRYYFREAMLNPGEIQVTRPYRSLIGKN
                     PCVTLSALIDRPSGRFVACVDLDWP"
     gene            complement(3011..4495)
                     /gene="amt"
                     /locus_tag="VFW59_00740"
     CDS             complement(3011..4495)
                     /gene="amt"
                     /locus_tag="VFW59_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5362773.1"
                     /translation="MRKLFALFALLAVLCGLSAAAYAEDAVVVDAVSAVVEAVPAVAA
                     PVAAPVAAAPVPNKGDTAFMLTASLLVIMMSIPGLALFYGGLVRSKNMLSILMQVFSI
                     FALITVLWAIYGYSLAFTTGNAFIGGFDRLFLKGVFDPATGAVANAATFSKGVVIPEF
                     AFVVFQATFAAITVALIVGAFAERMKFSAVLLFSALWFTFAYLPIAHMVWFWAGPDAY
                     TDQAAADAANATAGWLFQKGALDFAGGTVVHINAAIAGLVGAFMVGKRIGYGKEAMAP
                     HNLVMTMIGASLLWVGWFGFNAGSALEAGGTATLAFANTLIATAAAVLTWLAAEWMLK
                     GKPSLLGAASGAVAGLVAITPACGWVGIGGGLVIGALSGVVCFWGVTGLKKLLGADDA
                     LDVFGIHGIGGILGALLTGVFNTWSMGGTGIVDYVNNTIVDTTIGGQLWIQAQAVLTT
                     IVWSGVVAFVCYKIVDLTIGLRVKEEDEREGLDTASHGERAYNV"
     gene            complement(4506..4844)
                     /locus_tag="VFW59_00745"
     CDS             complement(4506..4844)
                     /locus_tag="VFW59_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5362774.1"
                     /translation="MKMVTAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEAAIEAGMLDQVLEAIEKAAQTGKIGDGKIFVSDLEQVIRI
                     RTGESGAEAL"
     gene            complement(4891..5649)
                     /locus_tag="VFW59_00750"
     CDS             complement(4891..5649)
                     /locus_tag="VFW59_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX5362775.1"
                     /translation="MKTKLLNSLILAALVVPSVAMAEEASPHTVSANVGFTTDYIFRG
                     IAQTSHDPAVQGGIDYAHSSGFYAGVWGSNVSWIKDSGAVATGNVTMELDTYLGFRNS
                     FAEDFSYDVGLVRYNYLGTYTPAAGFAKADTDEVYGSIGYKWVTAKYSYGLGNFLTLP
                     GAQGTNYIELNASVPVADSGFTVAAHVGKQTFKPAANAALTYTDYKLGVTKDIGGYVV
                     GLAYTSTNAGPGWTYATGGNWGKAVTALSVTHSF"
     gene            5889..6125
                     /locus_tag="VFW59_00755"
     CDS             5889..6125
                     /locus_tag="VFW59_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX5362776.1"
                     /translation="MLNQKFFEDMSAKLNEVVANSPAKDFEKNARALLAQGFSKLDLV
                     TREEFDVQAQVLARAREQLTALEARVAELEAQRK"
     gene            6274..>6761
                     /locus_tag="VFW59_00760"
     CDS             6274..>6761
                     /locus_tag="VFW59_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX5362777.1"
                     /translation="MSLAVLYSRALSGMDAALVTVEVHLANGLPSFTIVGLPETEVKE
                     SKDRVRAALQNCQFDFPARRITVNLAPADLPKESGRFDLPIALGILAATGQIPADRLN
                     DYEFAGELALTGELRPIRGALAMTYRAVSDGRAFMLPQANAAEAALVENATVYPAHTL
                     LQV"
BASE COUNT         1452 a         2079 c         1853 g         1377 t
ORIGIN      
        1 gccggaagag ctggtcgccg tggtcaatcg cgccttgcgc aagaacctgc ccgagcggca
       61 cgccaactgg gacgagttca tccgcgacct cggcaatgcc gcccacaaga tacgcatgtc
      121 cgattacgac ctcgacctgt atcgcggctt cagcatgtct acccagaccg tgctatcgag
      181 ctatatgtcg gcggatcggg acttctcccg cagcggcttc tcgcgcagcg tcatgcccgg
      241 ctcgttcgaa gcataggcgt cattgacccg cacgggcctg caaaacttcg cgatagaact
      301 tgtaggtatt cttcccgctc tgcttcgcca gatacatcgc ctcgtcggct tgcttgatca
      361 gcccgttcat atccccggag gaatcgggga agagggaggc gccgatgctc cctcccacat
      421 ggcactggcg gccctgtaaa tcaaagggct ccgacagcgt tgcgatcaac tttctggcaa
      481 tggtcgcaac atcctcttca gaatcgatgc cgttcagcac gaaggtgaac tcgtcgcccc
      541 ctacccgcgc caccgtatcc gaagcccgga ttacctcctg caatctcttg gctacgccct
      601 gcaacaacag atcccccgcg tcgtggccca gagtgtcgtt aacctgcttg aagccatcca
      661 gatcgagaaa caatgccgcg accttatatc cattgcgttt tgccagaagc atcgaatgcc
      721 cgagcgtatt catgaacatc gcccgattgg gcagatcggt cagaaaatcg taatgggcca
      781 gatgggcgat cttttcttcg gcctttttgc gttcggtgat gtcgcggacg atggcgacga
      841 aatatttttg cccgcccaag gtcatttccg agatggatat ctccattgaa aatgtctcgc
      901 cattcttccg caccgaagtc acttcccgat tgcgcatgcc cagaagctgg ctgtcacccg
      961 atttcagata gcgctgaaca tggacgtcat gcccgctccg gtccgattcg ggcatcagga
     1021 tcctgatatt ctggcccgtg atctcttcct gcgagtagcc gaatatgttc tcggcggcgg
     1081 ggttgaatcc ctgaatcgcc ccggattcat cggtggtgat gatgccatcc atcacattgt
     1141 gaataaccga ctgcagttgc agctggcttt tttccagctc gcccttgacc tgtttcaggt
     1201 tggtgaccat ctgccagaac agcttcactt cggggccgct cgccacccgc ttgtcgccaa
     1261 agaccttgct cgcctgctcg gcgatggtat cgtcatgcga caggttataa acgatgcagt
     1321 cgggataatc cttgcaggcc tgcaaggccg ccaccgggtc ggtgtaggtt ggaatgccgt
     1381 atttttttgc cagttgcagt ccaggagcct cggcgttggt atcgaccatg gcgacgaccc
     1441 tgaccagatc gtcttccata aacatttcga gcaatgcgga acctccgcgt ccggctccaa
     1501 caatcagcgc cgggtgccct ttcgttcttt ctgatgtcat gcttactccc tcaattgcca
     1561 ctgtgagcaa tttcacattg tcgattctgg cacataggct atattcgttc caattgtttc
     1621 atccttatcc catctcggaa aattggcaca gcgccatcat ccttgtcatg gccagtccag
     1681 gtccacacag gcgacaaatc ttccgctcgg cctatcgatc agcgccgaaa gcgtaacgca
     1741 tggattcttt ccaatcagcg aacgataggg gcgagtcacc tgaatttctc cgggattcag
     1801 cattgcctcc ctgaaataat atcgtctcga ccaattcgct tcggtggcct cggctagcgg
     1861 ccggtatcgc ggatcctcat attttggctt gctctcatga acgatgttct caccgatctg
     1921 atatccggcc tcgtccaaga agtacatacg catcacatcg ggcaaggcca gcaacctgcc
     1981 gcacagcgga acagcatcgg tcgcacccga cttgagctgc cgcaacagcg cgttgaactc
     2041 ggccatcggc ctatccattc tctccttgtg acggtttctg gcggcggcag aaaactcgac
     2101 atgcttgtcg cacagttgcg ccagcgtgtt gtcctgctca tgcgcatcta ccagctgctc
     2161 gcgcgggtag ccgaagtaat aaccctgtac cagatcgaca ttcgagtcca tggcgatcaa
     2221 tgcctgctgc tcggtttcga cgccttccat cagggtaatg caacccagct cctgaatcag
     2281 actcaccata ttgggcagtg ccttgcggac ggcgcgattg ctctccgcct gcacgacgat
     2341 ggagcgatcg aatttaacga tgtccggctg gatgttccag atgcggtcga aattggaatg
     2401 cgaggcaccg aaatcgtcga tcgcgacgag gcaacccaga tttttgtagt aattgacggc
     2461 ggcagacaag atggactcgt cgtaaatact tttttccaga atctcgatga caacctggcc
     2521 cggtggaatc tggctgtgtt ccagcatctc cttaaagaaa gagccatagc ttttccccag
     2581 gacggtgacc agcggatcga cattcagaaa gatccacgac ttgtcggaaa acacggaaaa
     2641 attctgcaca tgcacggcgc ggcataaacg gtcgagcagc acgttatccg actcatcttt
     2701 cacctgcccg aacaaggcga gcggcgatac cggcgagccg tcggcagcgc ttgcccggca
     2761 cagcgcctcg tagccgacgg ggctacggtg cgacaaactg aatatgggtt gaaacgcact
     2821 ggcgatactg aagtcgcgaa actgtgcggt tacatgtccg gtgttgccga cgtgggtgcg
     2881 gctgcaaacc cggtggaaat catggggtgg tgtattcatg aggttccctt tttgcgtttt
     2941 gagctctaaa aaaagagcgc cttgcggcgc tctgaaagaa gcccggggag ggcgggcttc
     3001 aggaggaaaa ttaaacgttg taagcgcgtt cgccgtggct cgccgtatcc agaccttcgc
     3061 gttcgtcttc ttctttcaca cgcagaccga tagtcaggtc gacaatcttg tagcagacga
     3121 aagccaccac accggaccag acgatcgtgg tcagcacggc ctgcgcctgt atccacaatt
     3181 ggccgccgat ggtggtgtcg acgatggtgt tgttgacgta atcgacgatg ccggtgccgc
     3241 ccatgctcca ggtgttgaac acgccggtga gcaaggcacc caagatgccg ccgatgccgt
     3301 ggataccgaa cacgtccagc gcatcatccg cgccgagcag tttcttcagg ccggtcacac
     3361 cccagaagca caccacgccg gacagcgcac cgatcaccag accgccgccg atacccaccc
     3421 agccgcaggc aggagtgatc gcaaccagac cggcaaccgc accggaagcc gcgcccagca
     3481 ggctaggctt gcccttcagc atccattccg cagccagcca ggtcagcacc gcagcagcgg
     3541 tcgcgatcag ggtgttggcg aatgccagcg tagctgtacc gcccgcttcc agcgcagaac
     3601 ccgcgttgaa accgaaccag cccacccaca gcagcgatgc gccgatcatg gtcatcacca
     3661 gattgtgcgg agccatagcc tccttgccat aaccgatgcg cttgcccacc atgaaggcac
     3721 ccaccagacc tgcgatagcc gcgttgatgt gcaccacggt accgcccgcg aagtccagcg
     3781 cgcccttctg gaacaaccag cccgcagtcg cattggccgc atcagccgca gcttggtcgg
     3841 tgtaggcatc gggacccgcc cagaaccaaa ccatgtgagc gataggcagg taagcgaatg
     3901 tgaaccacag cgcggagaac agcagcactg cggaaaactt catgcgctcg gcgaatgcac
     3961 ccacgatcag cgccaccgtg atcgcggcga aggtggcctg gaacacgacg aatgcgaact
     4021 cgggaatcac cacgcccttg ctgaaggttg cggcattggc gacagcgccg gtagccggat
     4081 cgaagacgcc cttcaggaac agacgatcga agccgccgat aaaggcgttg ccggtcgtga
     4141 acgccagcga atagccgtag atcgcccaca gcacggtgat cagcgcgaag atgctgaaca
     4201 cctgcatcag gatagacagc atattcttgc tgcgcaccag accgccgtag aacaaagcca
     4261 gaccggggat agacatcatg atcaccagca gcgaggccgt cagcatgaag gccgtgtcgc
     4321 ccttgttggg aaccggtgcg gctgcgacag gagcggcgac aggggctgca acggcgggca
     4381 ccgcctcaac cacagccgag actgcgtcga caacgacggc atcttcggca taggccgctg
     4441 cggacaggcc gcacagcacc gccagcaatg caaaaagtgc aaataattta cgcatcatgt
     4501 tctccttaga gtgcttctgc gccggactcg ccggtgcgga tgcggatgac ttgttcaaga
     4561 tcggatacga aaatcttgcc gtcgccgatc ttgccggtct gggctgcttt ttcgatggct
     4621 tccagcacct gatccagcat gccggcttcg attgccgcct ccagctttac cttgggcagg
     4681 aaatcgacca cgtattccgc accgcgatac agctcggtgt gacccttttg gcgcccgaaa
     4741 cccttgacct cggtaacggt gatgccctgc acgccgatgg cggacaacgc ctcacgcact
     4801 tcgtcgagct tgaacggctt gataatggcg gtgaccattt tcatgactct ctccttgtat
     4861 aagggcgtga cgaatcacgc ccgggttcct ttagaacgag tgggtaacgg acaatgcagt
     4921 aacggccttg ccccagttgc cgccagtagc gtaagtccag cccggaccgg cattcgtgct
     4981 ggtataggcc aagccgacta cataaccgcc gatatccttg gtcacgccca gcttgtagtc
     5041 cgtgtaagtc agcgccgcat tggcagccgg cttgaatgtc tgcttgccga cgtgagccgc
     5101 tacggtaaag ccgctatcag ccaccggaac gctggcattc agctcgatgt agttcgtgcc
     5161 ctgtgcgcct ggcaaggtca ggaagttgcc caggccgtag gaatacttcg cagtcaccca
     5221 cttgtagccg atcgagccgt agacctcatc ggtgtctgcc ttggcaaaac cggcagccgg
     5281 tgtgtaagta cccaggtagt tatagcgaac gagacccacg tcgtagctga agtcttcggc
     5341 gaagctgttg cggaaaccca aataggtgtc gagttccatc gtgacgttgc cggtcgctac
     5401 cgcaccgctg tctttgatcc agctgacgtt cgagccccac acgccagcgt agaatccgct
     5461 gctgtgagca tagtcgatac cgccttgcac agccgggtcg tgcgaagtct gtgcgatacc
     5521 gcggaagata tagtcggtgg tgaaaccgac gtttgcactg acagtatgag gagacgcctc
     5581 ttcggccatt gcaacgcttg gtacaaccag tgctgccaag atcagggagt tcaacagttt
     5641 cgttttcatc attcttcctt tctaaggttc taaacgggat aacagtatcc acgcctccct
     5701 tacagcagaa tgcgtgccag acaatatact cctttaatta cagataatta caaacaaccc
     5761 ttgattccgg gatcagcacg atgcctgctt ctggtgcgcc caaaagccat gctgcaccca
     5821 ataggtgcag cgacacatcg gctaaattgc taaacttcgg ccaacatcaa ttaccggagg
     5881 acggaaccat gctgaaccag aaatttttcg aagacatgtc ggccaagctg aacgaagtcg
     5941 tcgccaacag cccagccaag gatttcgaga agaatgcgcg cgcgctgctg gcgcaaggct
     6001 tctccaaact cgacctggtg acgcgcgaag agttcgacgt gcaggctcag gtgctggcgc
     6061 gcgcgcgcga acagctcacc gcgctggaag cccgcgtcgc cgaactggaa gcgcagcgca
     6121 agtaatttcg atgctgcggg ctttcgtcat tcccgcgcat gcgggaatcc agcaaaacaa
     6181 atactccgcg cagcggaccg agccagatat tgtcccgcgt tgcggggctg ctttaactga
     6241 ctggattccc gcctacgcgg gaatgacgga ctgatgagcc tcgcggtcct ctatagccgc
     6301 gcactctccg gcatggatgc cgcactggtc accgtcgagg tgcacctggc caacggcctg
     6361 ccgagcttca ccatcgtcgg cctgcccgag accgaagtga aggaaagcaa ggatcgcgtg
     6421 cgggccgccc tgcagaactg ccagttcgat ttccccgccc gccgcatcac cgtcaatctc
     6481 gcgcccgcag acctgcccaa ggaaagcggg cgtttcgatc tgcccatcgc gctgggcatc
     6541 ctcgccgcca ccggccagat tcctgccgac cgcctgaacg attatgagtt tgccggggag
     6601 ctcgcgctga ctggcgagct gcgcccgata cgcggcgcgc tggcgatgac ctatcgtgcc
     6661 gtgagcgacg gccgcgcctt catgctgccg caggccaatg ctgccgaggc cgcgctggtc
     6721 gagaacgcca cggtatatcc ggcgcacacc ctgttgcagg t
//
LOCUS       DASUIV010000021         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_562830, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000021 DASUIV010000000
VERSION     DASUIV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562830"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFW59_00765"
     CDS             <1..862
                     /locus_tag="VFW59_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5362778.1"
                     /translation="RGADAVLKMILLNGYFHADPHPGNVIFLPEDRIAFIDFGMVGRL
                     SHRRRDEIVDLLAAMAQRDEHAMMGVLIGWTGSVPVDEEQFAEDIGEIFFQYDHVVLK
                     DLNMSQLINDLMALIRNHGILLPADMAMLFKALITLEGLGRQLEPQFQLVEHLTPFVR
                     KLIIERYRPEALLKRGRETLSDGFAMLASLPRDIIRIGRDIKHGKFRINLDLKRLEQF
                     GHQLDKSVNRLTMGIVTGCLIIGSSIVMTVDGGPTLFGLPVFGFLGFMVAFFNSLWLI
                     WSIWLAGKEF"
     gene            971..1303
                     /locus_tag="VFW59_00770"
     CDS             971..1303
                     /locus_tag="VFW59_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEX5362779.1"
                     /translation="MNAMLVGADTLGNIPGVLDNFGISINKHLSGRNSSHQRKVDRLP
                     AGTDLLILFTDFLGHNVMKHFRQLANEENIRFIACRRSVCSLKQSLECVGLSERCASC
                     PNACHRKH"
     gene            complement(1373..>2348)
                     /locus_tag="VFW59_00775"
     CDS             complement(1373..>2348)
                     /locus_tag="VFW59_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5362780.1"
                     /translation="NAAIEAARAGESGRGFAVVADEVRKLAERVRVATEEISGNIDGM
                     LNLVSHTQEATARINTDTAQALDVVNRSSQHFGRMMSDFENMASGLTEIAGTMELFAG
                     TNLQVNRNVSEIHGLSTVVSTHLAQSEDVSVKLSVAAEQVQELVSRFVIGEGELDAVI
                     GVARHARDNLAEMLERAQGEGLNIFDQRYQPIPGSNPAKFHTAYDDKLAPALQKIYDQ
                     VVQKTNGGRFCLLVDTNGYAPTHNSFYSKPLTGDLQQDLVGSRDKRIFNDPTGIKSAK
                     NTQPFLLQTYMRDTGEILSEIALPIRLAGRHWGAIRVGFDPSALLQAD"
BASE COUNT          434 a          747 c          706 g          461 t
ORIGIN      
        1 acgcggcgcc gacgcggtgc tgaagatgat cctgctcaac ggctatttcc acgccgaccc
       61 gcaccccggc aacgtcatct tcctgccgga agaccgcatc gccttcatcg acttcggcat
      121 ggttggccgc ctgtcgcacc gtcgccgcga cgaaatcgtc gacctgctgg cggcgatggc
      181 gcagcgcgac gagcacgcga tgatgggagt gctgatcggc tggaccggca gcgtgccggt
      241 ggacgaggag cagtttgccg aggacatcgg cgagattttc ttccagtacg atcacgtggt
      301 attgaaggat ctcaacatgt cgcagctgat caatgacctg atggcgctga tccgcaacca
      361 cggcatcctg ctgccggcgg acatggcgat gctgttcaag gcgctgatca cgctggaggg
      421 actgggccgg cagctggagc cgcaattcca gctggtggaa cacctcaccc cgttcgtgcg
      481 caagctgatc atcgagcgct accgtccgga ggcactgctc aagcgcggcc gcgaaacgct
      541 gagcgacggc tttgccatgc tggccagcct gccgcgcgac atcatccgca tcggccgcga
      601 catcaagcac ggcaaattcc gcatcaatct cgacctgaag cgactggagc agttcggtca
      661 ccagctggac aagagcgtca accggctgac gatgggcatc gtcaccggct gcctgatcat
      721 cggctcctcg atcgtgatga cggtggatgg cgggccgacc ctgttcggcc tgccagtgtt
      781 cggttttctc ggcttcatgg tggcgttctt caacagcctg tggctgatct ggtcgatctg
      841 gctggccggc aaggagttct aggctacttc tttggcataa atgcaacgct ggcgccggcc
      901 atgcaacttt gagttgcatt tgcaaatgag aataaatatc attgttaaaa atacagtgaa
      961 aggtctcatc atgaatgcaa tgctggtcgg cgccgataca ctgggcaata ttcctggcgt
     1021 actggacaac ttcggtatct ccatcaacaa gcacctcagc gggcgcaaca gctcgcacca
     1081 gcgcaaggtg gaccgcctgc cggcaggtac cgacctgctg atcctgttca ccgacttcct
     1141 cggtcacaac gtgatgaagc atttccgcca gctggccaac gaggagaaca tccgtttcat
     1201 cgcctgccgc cgttcggtgt gctcgctgaa acagtcgctg gagtgtgtcg gcctgtccga
     1261 gcgctgcgcc agctgtccca atgcctgcca ccgcaagcac taggcccaaa tcacgcggcc
     1321 aaacaaaacg cccggatcgt cacgatccgg gcgttttgtc atcggtggtg tatcagtccg
     1381 cctgcaacag cgccgatggg tcgaaaccga cgcggatcgc accccagtgc cggccggcca
     1441 gccggatcgg caacgcaatc tccgacagga tctcgccggt gtcgcgcata taggtctgca
     1501 acaggaaggg ctgggtgttc ttcgccgact tgatcccggt cggatcgttg aagatgcgct
     1561 tgtcgcggct gccgaccaga tcctgctgca ggtcgccggt cagcggcttg gaatagaagc
     1621 tgttgtgcgt cggcgcatag ccgttggtat cgaccagcag gcagaaacgg ccgccgttgg
     1681 tcttctgcac cacctggtcg tagatcttct gcagtgccgg ggccagcttg tcgtcgtagg
     1741 cggtatggaa tttcgccgga ttggagccgg gaatcggctg gtagcgctgg tcgaagatgt
     1801 tgagaccctc gccctgcgcc cgctccagca tttccgccag gttatcgcgg gcgtgacggg
     1861 caacgccgat caccgcatcc agctcgccct cgccgatcac gaagcgcgac accagctcct
     1921 gcacctgctc cgccgctacc gacagcttca ccgacacgtc ttccgactgc gccaggtgcg
     1981 tggacaccac cgtgctcagg ccgtggattt ccgagacatt gcggttgacc tgcaggttgg
     2041 tgccggcaaa cagctccatg gtgccggcga tttcggtcag gccgctggcc atgttttcaa
     2101 agtcgctcat catgcggccg aagtgctgcg aggaacgatt gaccacgtcc agcgcctgcg
     2161 cggtatcggt attgatgcgc gcggtcgcct cctgggtgtg cgacaccaga ttgagcatgc
     2221 cgtcgatatt gccggagatt tcctcggtcg ccacccgcac ccgctccgcc agcttgcgca
     2281 cctcgtcggc caccaccgca aaaccgcgac cggattcgcc ggcgcgcgcc gcctcgatcg
     2341 ccgcattc
//
LOCUS       DASUIV010000022         4810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1120948, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000022 DASUIV010000000
VERSION     DASUIV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120948"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..624
                     /locus_tag="VFW59_00780"
     CDS             31..624
                     /locus_tag="VFW59_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5362781.1"
                     /translation="MNIQEIAEQLERTGYIVLDKALPDELLAALFARCHDDGSERFRA
                     AHIGRGADKKQINSIRGDVIQWLDEADSTDNAYLTLMDRLRLGLNEQLYLGLFDYECH
                     YAIYGAGTGYAKHLDALNGKKNRILTTVLYLNEDWQPRDGGELVVFDPTGEAVIATVT
                     PTFGKMIIFLSESFPHEVLTSHDTRRSIAGWFRVSGS"
     gene            complement(661..1539)
                     /gene="fliD"
                     /locus_tag="VFW59_00785"
     CDS             complement(661..1539)
                     /gene="fliD"
                     /locus_tag="VFW59_00785"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEX5362782.1"
                     /translation="MNINPNLAASTLAHFKFETLDSLIGESAGFNSVFADALSAESSS
                     AAGNLLNLFIQGAAKSGPHAGRNMALADPESGYRMMSLINDKDLLYKAQYSELSRMGD
                     AVRTMQQAGQSLGGISEASSNGDIRAGLQGFIGQYNDWMQGFNPDMQNGGLLAGTQAA
                     QLSRYELEQSVKNRFNGADDGVHGLADLGINADPVNGSLSLDSARLAEMLASNRHGAV
                     DAIREFSANFAKSASLLNADNNFIPNQLGNLDRAIHYIAANNTAWRAEFGTGNAAKPV
                     GQTAQALAAYQRNFGA"
     gene            1884..3905
                     /locus_tag="VFW59_00790"
     CDS             1884..3905
                     /locus_tag="VFW59_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5362783.1"
                     /translation="MKHDEFKTLLDARDEADLRQRTQQLALDLGFDYYLYATGLKTEN
                     STFSFTKVISTYPAAWIEKYVAEGFAAIDPAVRHCMERRTPFVWSMEAFERAGAGIMF
                     AEAQRHGIVSGVSIPLYDVASGSIGGIGLACRNEICRGLEDVGQVVLFALHFQEAFSR
                     LSVPFLPETHGVLSRREGECLGWISDGLSAAQIAQRLALDEAEVEQLLGQVMQKLDAS
                     SLPQAVAHAFAGGLLTCVPGHPVRPVTDEKASLAASPHYWALSEIERKITQAEAENNG
                     FSVGIMDLDHFRRINNALGHGAGNVLLAEVAERLRFNLPPKAQVSGLEGDAFLLLIPE
                     PASEQVANRLLNCLRSPLTVMDRLFTITASMGMVRYPQDGESAHALLRCADISLYRAK
                     ELGGDRVEFFAREMSQQAERSATLEVELRAALAREELILHYQPLVDSRTGRIRGLEAL
                     TRWPSPVLGTVPPDQFIPIAEQSDLIRILDEWVFITACRQLKVWRELGYDDLFVAVNV
                     CPSRFLNPAIVGHIVSTLDAIGLPPECLEIEITERMLMDSAPVVRERLAELRSHGVRF
                     AIDDFGTGHSSLSYLSSLPVDTLKIDRSFVQNVTANSEQSVLVEAIVAMADKLGLHVV
                     AEGIEQESEMSFLTGCGCDLLQGYLLGHPEPPETITSRLQAQSQSTCQP"
     gene            3970..>4810
                     /locus_tag="VFW59_00795"
     CDS             3970..>4810
                     /locus_tag="VFW59_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362784.1"
                     /translation="MTRTHPRHLVVSISGHGFGHVAQTAPVLNALHLLLPQLRITVRS
                     AVPLAHLRTRIHAPFEHLQSCGDIGMVMSSALDVRVEDSRNAYRDFHADWETRVADEA
                     RLLRELGADLVFSNVGYLPLAGAQQAGIPNVALCSLNWSDIYRHYCGQDEIAAQIQSC
                     YANADAFLRATPGMAMDAPSTGLRTGLTNLVPIEPIAAIARNQRDELNRLLKLSTDEK
                     LVLVSMGGIAGRLPMERWPRVEGVRYLVQAGWRVTHPDVITFESVPVSFSELLASSDA
                     LLCK"
BASE COUNT          924 a         1526 c         1439 g          921 t
ORIGIN      
        1 caatactggc gagacgggat cgatttaccg atgaacatac aagaaattgc cgaacaactg
       61 gaacgcaccg gctacatcgt gctggacaag gccttgccgg atgagctgtt ggcggcgttg
      121 tttgcccgct gccacgacga tggctccgag cggtttcggg ccgcccatat cggcagaggc
      181 gcggacaaga aacagatcaa ctcgatacgc ggcgatgtca tccaatggct ggatgaagcc
      241 gacagcaccg ataacgccta tctgaccctg atggacaggc tacgcctggg gctgaacgag
      301 cagctttatc tggggctgtt cgattacgaa tgccactatg ccatctatgg cgcaggcacg
      361 ggctatgcca aacatctgga tgcgctcaac ggcaagaaga accggatact gaccaccgtg
      421 ctgtacctga atgaggactg gcagccccgc gatggcggcg agctggtggt gttcgatccc
      481 accggcgagg cggtcatcgc aacggtcacg cctaccttcg gcaagatgat catcttcctg
      541 agcgaatcgt tcccgcatga ggtgctcacc tcgcacgaca cccggcgcag catcgccgga
      601 tggtttcgtg tcagcggcag ttaagccccg aagaaataca ctccgacgca acgaggcgca
      661 tcaggcaccg aaattccgct ggtaagccgc cagcgcttgc gctgtctgtc cgaccggctt
      721 ggcggcgttg cccgtgccga attcggcgcg ccatgcggta ttgttggcgg cgatgtaatg
      781 gatggcgcga tcgagattgc cgagttgatt cgggatgaag ttattatcgg cgttgagcag
      841 gctggcggac ttggcgaagt tcgcgctgaa ttcccgtatc gcatccaccg cgccatgcct
      901 gttcgaagcc agcatctcag ccagccttgc gctgtcgagc gagagcgagc cgttaacggg
      961 atcggcattg atgcccagat cggccaggcc atgtacgccg tcatccgcac cgttaaacct
     1021 gtttttgaca ctctgctcca gctcgtaccg ggagagctgg gccgcctgcg ttccggccag
     1081 caagccgcca ttctgcatat cgggattgaa gccctgcatc cagtcgttgt actggccgat
     1141 gaaaccttgc aggccagccc ggatatcgcc gttgctgctg gcttcgctga tgccgcccag
     1201 actctgccct gcctgctgca tcgtgcggac ggcatcgccc atccggctca actccgagta
     1261 ctgcgccttg tagagcaggt ccttgtcgtt gatcagcgac atcatccggt agcctgactc
     1321 ggggtctgcc agcgccatgt tgcggcctgc atgcgggccg cttttcgccg cgccctgaat
     1381 gaacaggttc agcaggttgc ccgctgcact actcgattcc gcagacagcg catcggcaaa
     1441 gacgctgttg aaaccggctg actcgccgat cagcgagtcg agcgtctcga acttgaagtg
     1501 cgccagcgtc gaagcggcca gattgggatt gatattcatg aggattcctc ctgcgttccg
     1561 gaaatttccg attgccttac agtcgcatcc tctcacaaca gatgatggcc gcatccgtga
     1621 aatagcgggc tatatctgcc gcatatgctg ccttcaccga caacgctcag cggcccggac
     1681 tgtccgcgac agcgcgcgcc agacatcgca tcaacgatag caattttcat cgaatggttt
     1741 tctgctgcta gaatgtggca aaaaaaacat gtgcagccat aagggagtcg caaaatttac
     1801 tgtcatccgg atgcgcctgg cccactgtcg gcccctgcct taatttcctc atcgttgact
     1861 ctccgtcaac gacactgccg cgcatgaagc acgacgagtt caagacgctg ctggatgccc
     1921 gtgacgaggc cgatctgcgc caacggacgc aacagctcgc gcttgacctg ggcttcgact
     1981 attacctcta cgccaccggc ctgaagaccg agaacagcac gttcagtttc accaaggtca
     2041 tctccaccta cccggccgca tggatcgaaa aatatgtcgc cgagggcttt gccgcgatcg
     2101 atccggccgt gcgccactgc atggagcggc gcacgccgtt cgtctggagc atggaagcct
     2161 ttgagcgcgc cggagccgga atcatgtttg ccgaggcgca acgccacggc atcgtctcgg
     2221 gcgtctcgat cccgttgtac gacgtcgcca gcggcagtat cggcggcatc ggcctggcat
     2281 gccggaacga gatctgtcgc ggactggaag atgtcgggca agtcgtgctg ttcgcgctgc
     2341 atttccagga ggcgttttcc cggctgtcgg tgcctttcct gccggagacg catggcgtgc
     2401 tttcccgccg cgaaggggaa tgcctgggat ggatttccga tggcctgagc gccgcgcaga
     2461 tcgcacagcg cctggcgctg gacgaggcgg aagtcgaaca gctgctgggc caggtgatgc
     2521 agaaactgga tgcgagctcc ctgccccagg cggtggccca tgcctttgca ggcgggctgc
     2581 taacctgcgt ccccggtcat ccggtgcgtc cggtgacgga cgaaaaagcc tctctggcgg
     2641 cgtcgccgca ctactgggcg ctcagcgaga tagaacggaa aatcactcag gcagaggcgg
     2701 aaaacaacgg cttttccgtc ggcatcatgg atctcgatca tttcaggcgc atcaacaatg
     2761 cgctggggca tggcgcaggc aatgtgctgc tggcggaagt cgccgaacgc ctgcgtttca
     2821 acctgccgcc caaggcgcag gtatccggcc tcgaagggga tgccttcctg ctgctgatac
     2881 ccgagcccgc cagcgaacag gtcgccaacc gcctgctcaa ctgcctgcgt tcgccgctga
     2941 ccgtgatgga ccggctgttc acgatcaccg caagcatggg gatggtgcgc tatccgcaag
     3001 acggcgaatc ggctcacgca ctgttgcgtt gcgcggatat ttcgctgtac cgcgccaagg
     3061 aactcggcgg cgaccgcgtc gaattcttcg cacgggaaat gagccagcag gccgaacggt
     3121 cggccacgct ggaagtggaa ttgcgcgcag cgctcgcacg cgaagaactg atcctgcatt
     3181 accagccgct ggtagacagc cgcaccggcc gcattcgggg tcttgaagcc ctgacacgct
     3241 ggcccagtcc ggtgctgggc acggttccgc cggaccagtt catcccgatc gccgaacagt
     3301 ccgacctgat ccgcatcctc gacgaatggg tattcatcac ggcgtgccgc cagctcaagg
     3361 tctggcgcga gctggggtac gacgatctgt tcgtcgcggt gaatgtgtgt ccgtcacgct
     3421 tcctgaatcc cgccatcgtc ggacacatcg tcagcactct ggacgccatc gggctgccgc
     3481 ccgaatgcct ggaaatcgag atcaccgaac gcatgctgat ggacagcgcc cccgtcgtgc
     3541 gcgagcggct ggccgaactc cgcagccacg gggtacggtt cgcgatcgac gatttcggca
     3601 ccgggcattc ctcgctgtct tatctcagca gcctgcctgt ggacacgctc aaaatcgatc
     3661 gatcatttgt ccagaacgtc accgccaaca gcgaacagtc ggtgctggtc gaggcgatcg
     3721 tggcgatggc cgacaagctg gggctgcatg tcgtggccga gggaattgaa caggaaagcg
     3781 agatgtcctt cctgaccgga tgcggatgcg atctgctcca gggttacttg ctcggccacc
     3841 ccgaaccgcc cgagaccatc acctcccggc tgcaggctca atcacagtcg acctgccaac
     3901 cctgacccga cgattcgacg cataattcca gaccgccgcg cccataagtc cgcctgcaac
     3961 cgaaactccg tgacccgcac gcaccccaga cacctcgtcg tctcgatctc cggtcacggt
     4021 ttcggccatg tggcccagac cgcgcccgtc ctgaatgcgc tgcacctgct cttgccgcag
     4081 ctgcgcatca ccgtacgctc ggcggtgccg cttgcccatc tgcgcacgcg catccatgcg
     4141 ccgttcgaac atctgcaaag ctgcggcgac atcggcatgg tgatgtcctc ggcgctggac
     4201 gtgcgcgtcg aggacagccg taacgcttat cgcgattttc acgccgactg ggagacgcgt
     4261 gtcgcagacg aggcccggct gctgcgcgaa ctcggcgcag acctggtgtt ttccaacgtc
     4321 ggctatctgc cgctagccgg cgcgcagcag gcgggcattc cgaacgtggc actgtgttcg
     4381 ctgaactggt ctgacatcta ccggcattac tgcggccaag atgagatcgc cgcgcaaatc
     4441 cagtcctgct acgccaacgc ggacgccttt ctgcgcgcca cgccgggcat ggcgatggac
     4501 gctccttcga ccgggctcag gacaggcttg accaatctcg tcccaatcga gccgatcgcc
     4561 gcaatcgcac gcaatcagcg cgacgagttg aaccgccttc tgaaactatc gaccgatgag
     4621 aagctggtgc tggtgagcat gggcggcatc gcaggtcgcc tgcccatgga gcgctggccg
     4681 cgcgtagagg gtgtgcgcta tctggtacag gcaggctggc gggtgacaca tcccgacgtc
     4741 atcaccttcg aatcagtgcc cgtcagtttc agcgaactgc tcgccagcag cgatgcgctg
     4801 ctgtgcaagc
//
LOCUS       DASUIV010000023         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_935508, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000023 DASUIV010000000
VERSION     DASUIV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935508"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..527
                     /locus_tag="VFW59_00800"
     CDS             159..527
                     /locus_tag="VFW59_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX5362785.1"
                     /translation="MKLHLANLGDTKMFTSHGEGYVMVNRERYDSSIVVSAEEVREDW
                     KVSGFDELNETHFAYFLALKPEVLLLGTGAQQRFPHPRLYRALTEAGIGVECMTTPAA
                     CRTYNILVAEDRKVIAAILL"
     gene            complement(717..>3678)
                     /locus_tag="VFW59_00805"
     CDS             complement(717..>3678)
                     /locus_tag="VFW59_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5362786.1"
                     /translation="PDIELPVVTVTANLEGAAPVQMETEVSRKIENAVASLGMVKHVY
                     ANITDGSAIVSVEFDLEKNVSEAVEDVRDAISRIRSDLPSDMKEPIISKVNTAGRAIV
                     TFSVSSEHMDEQELSWFVDNDISKKLLAVQGVGKFTRIGGVDREVRVEIDPLKVQALN
                     VSLADISRQIRKIQQESSGGRADIGGAEQAVRTIGTVRTVEELAAMDIVLPDGRHYRL
                     DQLATVTDAKAERRSKAMLDGKPLIAFEITRTKGSSEVSVANAVRIAVAKLNAEHPGV
                     HIGEAFDSVQRVQENFEGSMHLLYEGALLAVLVVWWFLRDARSTFVSAAALPLSVIPT
                     FIGMQYLGFTLNMVTLLSLALVVGILVDDAIVEVENIARHLHMGKSPYQAAMQATSEI
                     GLAVVATTFTLVSVFLPTAFMSGIPGKFFKQFGWTAALAILASLLVARLLTPMMAAYL
                     LKPMPETKEQHGKLMQWYLRQVEWCLAHRLATSFMAVAFFAGSLALIPLLPSGFVPPA
                     DRAQTQVTIELQPGTTLEQTASAAEEARAILMKVPEVKQVFTVVGRGASGDLFSAGSE
                     SDVRKANLNVTLVHRSERKLKQSEVEVLLREKLQVLPGMRVTVGTGDNGEKLQLLLAG
                     EDPVVLAEAVHAVERDLRTIRGLGNITSTASLVRPEISIRPDFARMADLGVTAESVGE
                     VVRIATAGDYSAALAKLNLPQRQVPIRVQMPESARAQIEVLEQLPVHGKAGNVKLGNV
                     ADITVSSGAAQINRLDRSRNVAINVELNGRQLGDVFAEVGKLPSLQALPPGVRRGQSG
                     DAERMQELFGSFGLAMLTGVMCVYIVLVLLFKDFLQPLTILAALPFAFGGAFVALLVT
                     HSSFSMPSLIGLLMLMGIVTKNSILLVDYAIAARREHGMSRFDALMDACSKRARPIVM
                     TTIAMGAGMLPIAMGLGAEPSFRMPMATAVIGGLITSTLISLLVVPVVFSYVDDLQGK
                     VRRLLRRDEGAVTR"
BASE COUNT          701 a         1242 c         1054 g          681 t
ORIGIN      
        1 agtatggcaa gcccgccatg ggcttcccca tgaaacatag tcgctacgct tcttgtttca
       61 tgtgaaaggg cgggatttta cctgcgccgc ccctgcacag caaatgccaa accgaaaatg
      121 gctcacataa tcggccacat aattggaggg ttagtcccgt gaagttgcat ctggccaatc
      181 ttggcgacac caaaatgttc acctcgcacg gcgagggtta tgtcatggtc aaccgcgaac
      241 gctacgacag cagcatcgtg gtatcggcgg aagaagtgcg cgaagactgg aaagtctccg
      301 gcttcgacga attgaacgaa acgcatttcg cctacttcct ggcgctcaag ccggaagtgc
      361 tgctgctcgg caccggcgcc caacagcgct tcccccatcc gcgcctgtac cgcgcgctga
      421 ccgaagcggg catcggcgtg gaatgcatga ccaccccggc ggcctgccgc acctacaaca
      481 tcctggtcgc cgaagaccgc aaggtcatcg cggcgatctt gctgtaaagc ctgaaaacaa
      541 actcgtccac gaaaaacacg aaaggcacga aaaaaatcag ttgactactc ggactcggca
      601 atccactcac cgagtgatgc agtcaatggt ttcatgcatt tgaattcgtt cgtgttcttt
      661 cgtgtctttc gtgtttttcg tggacagatg ttttttctga atcaagctcc cggctatcac
      721 ctcgtcaccg caccctcatc cctgcgcagc aaccgtctca ccttgccctg caaatcgtcc
      781 acgtagctga acacgaccgg cacgacgagc aggctgatta gcgtggaagt aatcaggccg
      841 ccgatcaccg ctgtcgccat cggcatgcgg aagctgggtt ccgcgcccag ccccatggcg
      901 ataggcagca tgcccgcgcc catcgcgatg gtagtcatca cgatggggcg cgcgcgtttg
      961 ctgcacgcat ccatcagcgc gtcgaagcgg ctcatgccgt gctcgcgccg cgccgcaatg
     1021 gcgtagtcca ccagcaggat ggagttcttg gtgacgatac ccatcagcat cagcagcccg
     1081 atcagcgacg gcatcgaaaa gctgctgtgc gtgaccagca gcgccacgaa cgcgccgccg
     1141 aaggcgaacg gcagcgccgc caggatggtc aacggttgca ggaagtcctt gaacagcagc
     1201 accagcacga tatacacgca catcacgccg gtcagcatcg ccaacccgaa gctgccgaac
     1261 agctcctgca tgcgctcggc atcgcccgac tggccgcgcc tcacgcccgg cggcaacgct
     1321 tgcagactgg gcaacttgcc cacttcggcg aacacgtcgc ccagttgccg cccgttcaac
     1381 tcgacgttga tcgcgacatt gcggctgcgg tccagacggt tgatctgggc cgcgccgctg
     1441 ctcacggtga tgtcggcgac gttgccgagt ttcacattgc ccgccttgcc gtgtaccgga
     1501 agctgctcca gcacctcgat ctgcgcgcgc gccgactccg gcatctgcac gcggatcggc
     1561 acttgccgct gcggcaggtt gagcttggcc agcgcggcgc tgtagtcgcc tgccgtggcg
     1621 atgcgcacca cttcgccgac gctctccgcc gtcacgccga gatcggccat gcgcgcgaaa
     1681 tccggccgga tgctgatctc gggacgcacc aggctcgcgg tggaagtgat gttgcccagc
     1741 cccctgatcg tgcgcagatc gcgctcgacc gcatgcacgg cttcggccag cacgaccggg
     1801 tcttcgccgg ctagcagcaa ctggagcttt tcgccgttat cgcccgtgcc gaccgtcacg
     1861 cgcatgcccg gcagcacttg cagcttttcg cgcaacagca cctcgacctc ggattgcttg
     1921 agcttgcgct cggagcgatg caccagcgtc acgttcaggt tggccttgcg cacatcggat
     1981 tcgctgcccg ccgagaacag gtcgccgctc gcgccccgcc cgaccacagt gaatacctgc
     2041 ttcacttccg gcactttcat caggatggcc cgcgcctctt cggcggcgct ggccgtctgc
     2101 tccagcgtgg tgccgggctg tagctcgatg gtgacttgcg tctgcgcgcg atccgcaggc
     2161 ggcacgaagc cgctgggcag cagcgggatc aacgcgagcg aacctgcgaa gaacgcgacc
     2221 gccatgaacg aagtcgcgag tcgatgcgcc agacaccatt ccacctggcg cagataccac
     2281 tgcatcagct tgccgtgctg ctcttttgtc tcgggcatcg gtttgagcag gtaggccgcc
     2341 atcatcggcg tcagcaagcg cgccaccagc agcgaggcca gaatcgccag cgccgccgtc
     2401 cagccgaact gcttgaagaa tttgccgggt atgccgctca taaaagcggt aggcaggaac
     2461 accgatacca atgtgaaagt ggtcgccacc acggccagcc cgatctcgct ggtcgcctgc
     2521 atcgccgcct ggtacgggct cttgcccata tgcaggtggc gcgcgatgtt ctcgacctcg
     2581 acgatggcat cgtccaccag aatccccacc accagcgcga gcgacagcag cgtcaccatg
     2641 ttcagcgtga agccgagata ctgcatgccg ataaaggtgg gaatcaccga cagcggcaac
     2701 gccgccgccg agacaaaagt ggaacgggca tcgcgcagga accaccacac caccagcaca
     2761 gcgaggagcg cgccttcata cagcaggtgc atggagcctt cgaaattctc ctgcacgcgc
     2821 tggaccgaat cgaacgcttc gccgatatgc acgccgggat gctcggcatt cagcttcgcc
     2881 accgcgatcc gcaccgcatt tgccaccgaa acttcgctgc tgcctttggt gcgggtgatc
     2941 tcgaaggcaa tcagcggctt gccgtccagc atggctttgg agcgtcgctc ggccttggca
     3001 tccgtcaccg tcgccagttg atccagccgg tagtggcgac cgtccggcag cacgatgtcc
     3061 atcgcggcca gttcctccac cgtccgcacg gtgccgatgg tgcgcaccgc ctgctccgca
     3121 ccgccgatgt cggcacgacc gccggacgat tcctgctgga tcttgcggat ctgccgcgag
     3181 atgtcggcca gcgacacgtt cagcgcctgc accttgagcg gatcgatctc gacgcgcact
     3241 tccctgtcca ccccgccgat gcgggtgaac ttgcccacgc cctgcacggc gagcagtttt
     3301 ttggatatgt cgttgtccac aaaccacgac agctcctgct cgtccatgtg ctcggaggaa
     3361 acgctgaacg tgacgatcgc gcggcctgcc gtattcacct tggagatgat cggctctttc
     3421 atgtcgctgg gcaggtcgga gcggatgcgg cttatggcat cgcgcacatc ctccaccgcc
     3481 tcgctgacgt tcttttccag atcgaactcg accgagacga tggccgagcc gtcggtgatg
     3541 ttggcataca catgcttcac catgcccaga ctcgccaccg cgttctcgat cttgcgcgag
     3601 acttcggtct ccatctgcac cggtgcagcg ccttccagat tcgcggtcac cgtcaccacc
     3661 ggcaactcga tgtcgggc
//
LOCUS       DASUIV010000024         6852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_935694, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000024 DASUIV010000000
VERSION     DASUIV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935694"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VFW59_00810"
     CDS             complement(<1..1052)
                     /locus_tag="VFW59_00810"
                     /inference="COORDINATES: protein motif:HMM:NF022448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BcsE family c-di-GMP-binding protein"
                     /protein_id="HEX5362787.1"
                     /translation="MNEDAVPEYLLGIGNLPDVTGKMLAGRLYVVMHDADLGLEVVCR
                     TVVAASASGRVDWLAEVPSAYLGVVPQLGREVADCLREQTLRIFQSTSENPHGMCRRI
                     LKDLDLIGVAGGGLVIADGADRFIEEVSDEAWEGDLSAWQKWAERTGCAVLWMCPRRA
                     GQFGHEADLLRMAHRFSGMARLRKSGDELRWDVYYWFGNEGLMLDKSFRLNADDSGSC
                     WVEEHHSVPAGEAELIVDEDEVYIMKAALPAGRSTPSGWRVFDTVEQMSVALSSARAP
                     TVIFHHNPGGSFETLARAVFELRRAIGAYVKIVIKETGGRLRHSHEQLLLSLGANLTI
                     PGETGFVHMLSLLRSI"
     gene            1369..2133
                     /gene="bcsQ"
                     /locus_tag="VFW59_00815"
     CDS             1369..2133
                     /gene="bcsQ"
                     /locus_tag="VFW59_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090540 - bacterial cellulose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HEX5362788.1"
                     /translation="MKVVSVTGINGGVGATTVVAGLASMLSARKWQVIVLDLSPHNTL
                     RLHFGMPWDEGNGLVPQILSGKPWHEAAYLCENGVSFLPFGKCGEQDIATFNRQLRQE
                     PDWLSSHLSELEDSDETIILIDCPRTEGPLRSQAHALSDQILVVMEAGPLSYAALAAP
                     GTIFAATDAPKATYLLNSFNPTLTLDRDIAQLLRVEQGERLCPATIHRDEFVREALAS
                     KLSLDAYAPYSQAADDFTTLSIWLVAKFAHLELASA"
     gene            2130..4700
                     /gene="bcsA"
                     /locus_tag="VFW59_00820"
     CDS             2130..4700
                     /gene="bcsA"
                     /locus_tag="VFW59_00820"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF008558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HEX5362789.1"
                     /translation="MSMAAFPPLRQNFRALLRACGIDPDQSPARLLLSLLFILPPPKP
                     AGYYAKFFPHVRFDRLRPGDIIRIPLQLLWLLVIRNAPLQRRPLRINFWLTPYRRLRT
                     VISNGIERLTERLHLKSDTDIEHFQEISEAYAKHRYWDHPLVRYLAYAASALLSFLCI
                     TTPFSTTSQLIFVSLLWIIALIIRRVPGQVITLLLMVLSVTASTRYLWWRITSTLNWD
                     SAFDLSWGILLIMAEIYTWLILLFGYIQTAWPLKRPPVMLPADIATWPTVDVFIPTYN
                     EPLKVVKPTIHAAMGMDWPREKLNVHLLDDGRRAEFRELAQQLGVNYIARPTNEHAKA
                     GNLNYALTKTDSEFIVIFDCDHIPTRSFLQLCMGWFLRDPKLALVQTPHHFYSADPFE
                     RNLDVFREAPNEGELFYGLIQDGNDMWNATFFCGSCAILRRAPLEEIGGIAVETVTED
                     AHTAMKLQRRGYNSAYINIPQAAGLATESLSAHIGQRIRWARGMAQIFRLDNPFLGKG
                     LKWAQRLCYSNAMLHFFNGIPRLIFLTAPLAFLLFHAYVIYAPAISVALYVLPHMAHA
                     TITNSRTQGKYRNSFWAEIYETVLAWYIARPTFVALLNPHKGKFNVTAKGGAVDSEYF
                     DWSISLPYLILIGLNVTGVCAGIWQLSYGPSDEIPTLILNLFWVSYNLIVLGAAAAVA
                     LESRQVRVSHRVQMKLSAVLHLANGKRIHCDTADFSEGGVALVPAAIPDVKRDESVSV
                     SLWHGDEELAFPARIIAIVPPQLRLKWELQSQEQESALVQCTFARADAWVDWAKDRPA
                     DKPLESLGEVLSLGVLGYRRILEQFLPSWTPTMRRFNTVKAFMASFLPRSPHLQENHR
                     "
     gene            4697..>6852
                     /gene="bcsB"
                     /locus_tag="VFW59_00825"
     CDS             4697..>6852
                     /gene="bcsB"
                     /locus_tag="VFW59_00825"
                     /inference="COORDINATES: protein motif:HMM:NF008323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HEX5362790.1"
                     /translation="MKNLFTTLLCCLLSAHAAFAVARQSVDSPEEVTKQPMPLVVKRL
                     TLNQLSNRSAINLRGIESSDTLTLGGRRDELVTRAVLHLSYTYSPALIPGESHLKFLV
                     NDEVVHVLPVTKENAGRKVTQDLEIDPRLFSNLTRLKFEFIGHYTHDCEDPFHSSLWM
                     EINGASQLELTVRPLALKNDLALLPEPFFNARDFNNQLVIPFVFSATPDNDTLNAAGI
                     VSSWFGKLAAWRGARFPSKLDSLPPGHAIVFASNDARPGFLRDYPKITGPALEMITNP
                     ADERSKLLLILGRNGAEQKIAAQALVLGNAAWAGSHVSIKNIQQEPVRKPYDAPAWVR
                     MDRPMKFGELVASAQDLQVAGYAPQPIRIPLRLPPDLFTWRSRGVPIDLKYRYTPPLR
                     LSESRLRMSVNGELVKSFTLLPTGDAEKNRIRLPLIDDMLFGQSQELMLPSFKLGTRN
                     ELLFQFSTAAQKEGLCRDYFNENVRDMIDADSKIDFSEFPHYAEMPNLSYFATAGFPF
                     TKYADLSQTIVVLPDQPTAYDIETMLTLLGRMGESTGYPATQFGIAHANEHDRLKNAD
                     LLVIGASLKQGLLTQWADKLPVDIQGGALRNSQPKRAASFLFDWFNFETLPDPGIMAQ
                     QQFGQNGSLAAILGFESPLSAQRSVVAVVSTQPEGLTQTLDALERSNNNIQGSAVFIH
                     PDKVEGFLVGQTYFIGELPFWTSIWYPFANHPVLLA"
BASE COUNT         1447 a         2225 c         1781 g         1399 t
ORIGIN      
        1 tgtatggatc tcagcagact cagcatgtgt acaaaacccg tttcaccggg aatcgtcagg
       61 ttcgccccca gactcaacaa cagctgctcg tggctatgcc gcaggcggcc tcccgtctcc
      121 ttgatcacaa tcttgacgta agcgccgatg gctcgcctca actcgaacac cgcgcgcgcc
      181 agcgtttcaa aagagccgcc cggattgtga tgaaagatca cggtaggcgc acgggcagac
      241 gagagcgcca cgctcatctg ctcgacggta tcgaacaccc gccaaccgct tggagtggaa
      301 cggccagcgg gcaaggctgc cttcatgatg taaacctcgt cctcatcaac gatgagttcg
      361 gcttccccgg caggcacgct gtgatgttct tcgacccaac aactgccgct gtcgtcggca
      421 ttcaagcgga acgacttgtc cagcatcaga ccttcatttc cgaaccagta ataaacatcc
      481 caacgcaact cgtctcctga cttgcgcaaa cgcgccatgc cggagaaccg gtgtgccatc
      541 cgcagcaaat cggcttcatg tccaaattgg ccggcccgcc ggggacacat ccacaagacc
      601 gcacagccgg tacgttcggc ccatttctgc caggcagaca ggtcaccttc ccaagcttcg
      661 tcagagacct cctcgatgaa cctgtctgcg ccatcggcta tgaccagccc acctcctgcc
      721 acaccgatca agtccagatc cttcaatatc ctgcgacaca ttccatgggg attttccgac
      781 gtcgactgaa agattcgtaa cgtttgctca cgcaggcaat cggcgacctc gcgtcccagc
      841 tgaggcacga caccgagata tgccgacggc acttcggcga gccaatccac acgcccggaa
      901 gcactggctg cgaccacggt tcggcagacc acttccagtc ccaggtcggc atcatgcatc
      961 acgacataaa gccgcccggc aagcatcttg cctgtcacgt cgggcaaatt tccaattccg
     1021 agcagatatt ccggcacggc atcctcattc acaaaaatgt tcgagtattt tacctttgac
     1081 tcgcgccaaa aggtaaaatt accagagcgt tgagtagctc gcaccccata cggccccgtt
     1141 taaaaagtta atgaagccag tcagatcagt ctgattataa tttgtacatg catgcacact
     1201 ccgacatcgc cgaactcttc tcaaaactga gtgacgataa aaaacaatac cgggagatcg
     1261 ctcacacccg aaagcaggaa gaggccggct tacggtgggc gttgctgaac gaaattcacc
     1321 gttcaatctc cccaacggaa ccgggcaagc gcgaggagtc gcgttagcat gaaagtcgtt
     1381 tcggttacgg gaataaacgg cggcgtcggc gcaaccaccg tggtggcagg attggcatcg
     1441 atgctctccg cacgcaaatg gcaagtcatc gtgctcgatc tctctccgca caacacactc
     1501 aggctgcact tcggcatgcc atgggatgag ggtaacggtc tcgtccccca aatattgtcc
     1561 ggaaagccct ggcatgaggc tgcttacctt tgcgagaacg gcgtgagctt cctgcctttc
     1621 ggaaagtgcg gcgagcaaga catcgccacc tttaatcggc agctgcgaca agaaccggac
     1681 tggctgtcgt cgcatctgag cgaactcgag gatagcgacg aaacgatcat cctcatcgac
     1741 tgtccgcgca ccgaaggacc attgcggtcg caggcgcatg ctctttccga ccagatactc
     1801 gtcgtcatgg aggcaggccc cctctcctat gccgcgcttg ctgcccccgg cacgatcttc
     1861 gcggcaacgg atgcccccaa agcgacttac ctgctcaact cgttcaaccc cacgctgaca
     1921 ctggatcgag acatcgcgca attactgcgg gtcgagcaag gagaacgcct gtgtcccgcg
     1981 accatccacc gtgacgaatt cgtacgcgaa gcattggcca gcaagctcag tctcgatgcc
     2041 tatgcgcctt acagccaggc cgccgacgac ttcaccaccc tatccatctg gctggtcgcc
     2101 aagttcgctc atctggagct cgcgtcagca tgagcatggc cgcattccct ccattacggc
     2161 agaatttccg cgcactcttg cgggcatgcg gcatcgatcc ggaccagtcc ccggctcgcc
     2221 tgctgctgtc tctgctgttt attttgccgc cgcccaaacc ggccggctat tacgcgaaat
     2281 tctttccgca tgtccgcttt gatcggctgc ggccgggcga tatcatccgt attccgctgc
     2341 aactgctctg gctattggtg atccgcaacg ccccgctcca gcgtcgcccc ctgcgaataa
     2401 acttctggct gacgccctac cgccgactac gcaccgtcat aagcaacggg atcgaacgac
     2461 tgaccgagcg gctgcatctc aaaagcgata cggacatcga acactttcag gagatcagcg
     2521 aagcctatgc gaaacaccgc tactgggatc atccgctcgt ccgttacctg gcctatgccg
     2581 cttcggcact gctctccttt ctttgcatca ccaccccttt cagcacgact tcgcaactga
     2641 ttttcgtttc gctgctgtgg ataatcgcgt tgatcatacg gcgtgtcccc gggcaggtga
     2701 tcacactcct gctgatggtc ttgtcggtta ccgcctcgac tcgctatctg tggtggcgga
     2761 tcacctcaac cctgaactgg gacagcgcat tcgacctgag ctggggcatc ctgctgatca
     2821 tggccgagat ctacacctgg ctcattctgt tgttcggtta catccagacg gcatggccgc
     2881 tcaaacgccc gccggtgatg ctgcctgccg acatcgcgac atggcccacc gtggatgtat
     2941 tcatccccac ctacaacgag ccgctcaaag tcgtcaaacc caccattcat gcggccatgg
     3001 ggatggactg gccccgggaa aagctcaacg ttcacctcct cgatgatggc cgtcgcgcag
     3061 agtttcgcga gcttgcgcaa cagctgggcg tcaattacat cgcccgcccc accaacgaac
     3121 atgccaaggc gggcaacctc aactacgcct tgaccaagac cgacagcgaa tttatcgtca
     3181 ttttcgactg cgaccacatt ccgacccgtt ccttcctgca actgtgcatg ggctggttcc
     3241 tgcgcgatcc gaagctggcc ttggtgcaaa cgccgcatca tttctattcg gcggatccgt
     3301 tcgaacgcaa tctggatgta ttccgcgagg caccgaacga aggagagctg ttctacggtt
     3361 tgatccagga cggcaacgac atgtggaatg ccactttttt ctgcggctcc tgcgccattc
     3421 tgcgccgcgc tccattggaa gagattggcg gcatcgctgt tgagacggtg actgaagatg
     3481 ctcataccgc catgaaactg caacggcggg gatacaactc ggcctacatc aacatcccgc
     3541 aagcggcagg actggccacc gagagccttt ccgcccatat cggccaacgc atccgctggg
     3601 cgcgcggcat ggcgcagata ttccgcctgg acaacccctt cctgggcaag gggctgaaat
     3661 gggcgcagcg cctgtgttac agcaatgcca tgctgcactt cttcaacggc atcccgcgcc
     3721 tcatcttcct cacggcgccg ctcgccttcc tgctgttcca cgcctatgtg atctacgcgc
     3781 cggccatttc ggtcgcactt tatgtgctcc cccacatggc acatgccacc atcaccaatt
     3841 ccagaacgca gggaaaatat cgcaactcgt tctgggctga aatctacgaa accgtgctgg
     3901 cctggtacat cgcccgtccg actttcgtgg cactgctcaa tccgcacaaa ggaaagttca
     3961 acgtgacggc caagggcggg gccgttgaca gcgaatattt cgactggtcc atctccctgc
     4021 cctatctgat cctgatcggt ctgaatgtga cgggggtttg cgcgggcatc tggcaactca
     4081 gttacggccc gagcgacgag attccgaccc tgatcctcaa tctgttctgg gtgagctata
     4141 acctcatcgt cctcggcgct gccgcagcgg tcgcgctcga atcccgacaa gtcagggtat
     4201 cgcaccgcgt acagatgaaa ctctcggcag tgctgcatct cgccaatggc aaacgcattc
     4261 attgcgacac cgccgatttt tcggaagggg gcgtcgcgct cgtgccggca gccatacccg
     4321 acgttaagcg agatgaaagc gtctcggtct cgctctggca tggcgacgaa gagctggcct
     4381 tcccggcgcg catcatcgcc atcgtcccgc cgcaactgcg tttgaaatgg gagttgcaaa
     4441 gccaggagca ggagtccgct ctggtgcaat gcactttcgc ccgtgcagat gcctgggtcg
     4501 actgggccaa ggatcgcccg gccgacaaac cgctcgagag cctgggagag gtactctctc
     4561 tgggcgtgct gggttaccgc cgcatccttg agcaatttct ccccagctgg accccgacca
     4621 tgcgccggtt caataccgtc aaggccttca tggccagctt cttgccacgt tcaccccacc
     4681 ttcaggaaaa tcatcgatga agaatttgtt caccaccctg ctctgctgcc tgctgtccgc
     4741 ccacgccgcc ttcgcggtgg ctcgacagag cgtcgattcg cccgaagagg ttactaaaca
     4801 gcccatgccg ctggtggtga aacgcctcac gctgaatcag ctcagcaacc gcagcgcgat
     4861 caatctgcgt ggcatcgaga gcagcgacac cctcaccctc ggcgggcggc gcgacgaatt
     4921 ggttacccgc gcggtattgc acctgagcta cacctattcc cccgcgctga ttcccggcga
     4981 atcccatctg aagtttctgg tcaacgatga ggtcgtccat gtcttgcccg tgaccaaaga
     5041 aaatgcggga cgcaaagtca cacaggatct ggagatcgat ccgcgcctgt tcagcaatct
     5101 cacccgatta aaattcgagt tcatcggaca ttacactcat gattgcgaag atccgttcca
     5161 ctccagcctg tggatggaaa tcaacggcgc cagccagctg gagctgaccg tacgtccgct
     5221 ggcgctcaag aacgatctgg cgctgctgcc cgaaccattc ttcaatgcgc gtgacttcaa
     5281 taaccagctg gtgattccat tcgtcttttc tgcgacgcct gataacgaca cgctgaatgc
     5341 cgccggcatc gtgtcgtctt ggttcggcaa gctggccgca tggcgcggtg cgcgcttccc
     5401 ctcgaaactg gacagccttc cgcccggaca tgccatcgtc tttgccagca acgatgctcg
     5461 cccgggtttc ctgcgcgact atccaaaaat aaccggtccg gcgctcgaaa tgatcaccaa
     5521 cccggccgat gaaagatcga aattgctgct gattctcggc cgcaacggcg ctgaacagaa
     5581 gattgccgca caggccctgg tgctgggcaa tgcggcatgg gcaggcagtc acgtatccat
     5641 caagaacatc cagcaagagc ctgtccgcaa accttacgat gcccccgcct gggtgcgcat
     5701 ggaccggccc atgaaattcg gcgaactcgt cgcatcggcg caggatttgc aggtcgcagg
     5761 ctatgccccg cagcccattc gcattccgct gaggctcccg cccgatctct tcacctggcg
     5821 cagccgtggt gtgccgatcg acctgaaata ccgttatacc ccgccgctcc gtctgagcga
     5881 atctcgcctg cgcatgagcg tcaacggcga actggtcaag tcgttcaccc tgctaccgac
     5941 tggggatgcc gagaaaaacc gtatccgcct gccgctgatc gacgacatgt tgttcggcca
     6001 gtcacaggaa ttgatgctgc catcgttcaa gctcggcacg cgcaacgaat tgctgttcca
     6061 gttcagcact gcggcccaga aggaaggcct gtgccgggat tacttcaacg agaacgtgcg
     6121 tgacatgatc gatgcggact cgaagatcga tttttcggaa tttccgcact acgcggaaat
     6181 gcccaatctg agttacttcg ccacggcggg cttccctttc accaagtatg cggacctttc
     6241 gcaaacgatc gttgtcttgc cggatcagcc gaccgcctac gacatcgaaa ccatgctgac
     6301 cctgctcgga cgcatgggcg aatcgacggg ctacccggct acccagtttg ggatcgccca
     6361 cgccaatgaa cacgaccgcc tgaaaaatgc cgacctgctg gtgatcggcg cgtcactcaa
     6421 acaggggctg ctgactcaat gggcagacaa actgcccgtc gatatacaag gtggggcgct
     6481 gcgcaacagc cagcccaaac gggctgccag ctttctgttc gactggttca acttcgagac
     6541 gctccccgac cccggcatca tggcgcagca gcagttcggg cagaacggct cgctggcagc
     6601 catactggga ttcgaatcgc ccctctccgc gcagcgtagc gtcgtcgcgg ttgtctcgac
     6661 ccaacccgaa ggtctcacgc aaacgctgga tgccctcgag cgctcgaaca acaacattca
     6721 gggcagcgcc gtgttcatcc atcccgacaa ggtagaggga ttcctggttg gccagaccta
     6781 cttcatcggc gaactgccgt tctggacctc tatctggtat ccctttgcga accatcccgt
     6841 cttgctcgcc at
//
LOCUS       DASUIV010000025         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_749741, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000025 DASUIV010000000
VERSION     DASUIV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749741"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /gene="dacB"
                     /locus_tag="VFW59_00830"
     CDS             complement(<1..1100)
                     /gene="dacB"
                     /locus_tag="VFW59_00830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX5362791.1"
                     /translation="MRFLFFVLLFAAVAADAASLPDAVRQSLQQAGIPLEGVAVEVRE
                     VGARRPLISLNAARPMNPASTMKLLTTYVGLDLLGPAYTWKTEAYLDGELKDGVLDGD
                     LVLKGYGDPKFTVEQFWLWLAELRARGLRDIRGDLVLDRSFFALPAFDSGAFDNDPVR
                     AYNVGPDALLLNFNTLHLRYLPDGNSMKLISELPLDGVTLDNRLVPRSSGGCDGWDDT
                     ISVQQKGDSVVFQGGYPGECGERDHNLSVMPHTRYVEAVFRALWKELGGTLRGKQREG
                     VASANARLFATHRSEPLSSVIRDINKFSNNVMARQLFLTLGASPSSAATIPQSIKVMK
                     DWLAQKRLNFSELELENGAGLSRRERISAQHLA"
     gene            complement(1097..2521)
                     /locus_tag="VFW59_00835"
     CDS             complement(1097..2521)
                     /locus_tag="VFW59_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5362792.1"
                     /translation="MSHRTSSNATLLSRSVSAVWHPCSQMKHYESFPLVPIARGQGVW
                     LHDFDGNRYLDGVSSWWVNLFGHCNPRINAALVDQLGRLEHVMLAGFTHEPVVQLSER
                     LRELAPAGLGHCFYGSDGASATEIALKMSAHYWRNSGQPGKARFISLQNSYHGETLGA
                     LSVTDVALFRDAYGALIAQQATVPSPDWRCAAPGESAEQYALRCADALEAHLQQHHSE
                     LAALIIEPLVQGAAGMAMYHPAYLQRARELCTRYGVHLIADEIAVGMGRTGTMFACEQ
                     AKPLSLTLSPEGRGDKGSDVLPSPVYGRGVGGEGMTPDFLLISKGITGGYLPLSIAMT
                     TDEIYRAFYADETARGFLHSHSYTGNPLACRAALATLDIFAQDEVLNANRDKAAYLNR
                     IAQPLRDHPKVRNFRNTGMIWAFEVDAPHGDFARRCFALGLQKELLLRPMGNTVYFMP
                     PYVISEEEMRLLVTRTLEIVEELT"
     gene            2608..3519
                     /locus_tag="VFW59_00840"
     CDS             2608..3519
                     /locus_tag="VFW59_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX5362793.1"
                     /translation="MHILITGGTGLIGRRLCQALLAEGHELTVLSRKPDSVAGKCGAR
                     VHAMASLHDWHPSQTFDAVINLAGEPIVDERWSAKRKQLLWDSRVTLTENLVRRLAAA
                     ERKPTVLLSGSAVGYYGNGGDRMLDESSDAGEGYAAELCKAWEDAARVAESTGVRVCL
                     LRTGLVLSRDGGLLERMVPPFKLGLGTRVGNGKQWMSWVHIEDCIAILLCLLHDEQMR
                     GPYNVTAPQPVSNADFTKVLAGVLRRPAPFAAPAALLNMSMGESASLILEGQRVLPRK
                     LEVAHYRFQFSALREALEDLLDQTPAR"
     gene            3644..4897
                     /locus_tag="VFW59_00845"
     CDS             3644..4897
                     /locus_tag="VFW59_00845"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5362794.1"
                     /translation="MKRVDDFRLRFGKREIVPIMIGGMGVDISTAELALEAARLGGIG
                     HISDAMLPTVTDRLYDTSFVKEKLQQYKYNIDNADKSAVKFDLGHVAESTRLHVSKTM
                     EAKRGEGMVFLNCMEKLTMNAPRETLTVRLRSAMDAGIDGITLAAGLHLGSFAMIEDH
                     PRFRDVKLGIIVSSLRALQLFLRKNARTNRLPDYIVVEGPLAGGHLGFGLEDWMKYDL
                     RTITNEVLQYLRDEKIDIPVIPAGGIFTGSDAVSFLEEGSAAVQVATRFTVAEECGLP
                     KDVQQEYFKASEHDIEVNGVSPTGYPMRMLKNSPAIGSGIRPGCESYGYILDANGKCS
                     YIDSYNREVALHPGEKKISVMDKTCLCTHMRNFKLWTCGHYTYKLKDTTHKLADGSYQ
                     VLSAEHIFHDYQFSTDNQVALPPQK"
BASE COUNT          965 a         1612 c         1500 g          876 t
ORIGIN      
        1 gccagatgct gcgcgctgat gcgctcgcgg cgcgacagcc cggcaccgtt ctccaattcc
       61 agttcggaaa agttcagcct tttctgcgca agccaatctt tcatgacttt tatgctctgc
      121 ggtatcgttg ccgcagagga cggcgatgcc cccagcgtca ggaacaattg ccgcgccatg
      181 acgttgttgc tgaatttgtt gatgtcgcgg atcacgctgg agagcggctc ggagcgatgg
      241 gtcgcgaaca gccgtgcatt cgcgctcgcc acgccttcgc gctgcttgcc gcgcagagtg
      301 ccgcccagtt ccttccacag cgcgcggaac accgcctcga cataacgcgt atgcggcatc
      361 acgctgaggt tgtggtcgcg ctcgccgcat tcgcccggat agccgccctg gaacaccacg
      421 ctgtcgccct tctgctgcac gctgatcgta tcgtcccagc cgtcgcaacc gccggaggat
      481 cgcggcacca gccggttgtc cagcgtcacg ccatccagcg gcagttcgct gatcagcttc
      541 atgctgttgc cgtccggcag atagcgcagg tgcagcgtgt tgaagttcag cagcaacgca
      601 tctggaccga cgttgtaggc gcgcaccggg tcgttgtcga acgcgcccga gtcgaacgca
      661 ggcaatgcaa agaagctgcg atccagcacc agatcgcccc tgatgtcgcg caggccgcgc
      721 gcgcgcaact cggccagcca gagccagaac tgttcgacgg taaatttcgg atcgccgtag
      781 cccttcaaca ccagatcgcc gtccagcacg ccatccttga gctcgccgtc cagatacgcc
      841 tcggtcttcc aggtgtaggc ggggccgagc agatccagcc cgacataggt ggtgagcagc
      901 ttcatcgtcg aagccgggtt catcggccgc gcggcattca ggctgatcag cggcctgcgc
      961 gcgcccacct cgcgcacctc gacggcgacg ccctcgagcg gaatacccgc ctgctgcagc
     1021 gactgcctga cggcatcggg caagctggcc gcgtcggctg ccactgccgc gaacaacagt
     1081 acaaagaaca gaaacctcac gtcagctcct ctacaatttc cagcgtgcgc gtcaccagca
     1141 gccgcatctc ttcctcgctt atcacatagg gcggcatgaa atagaccgtg ttgcccatcg
     1201 ggcgcagcag cagttccttt tgcaagccga gcgcgaaaca gcgccgcgca aaatcgccat
     1261 gcggtgcgtc cacctcgaat gcccagatca tcccggtgtt gcgaaagttc cgtaccttcg
     1321 gatgatcgcg cagcggctgg gcaatgcggt tcaggtacgc cgccttgtcg cggttcgcat
     1381 tcagcacctc gtcctgcgcg aagatatcca gcgtggcgag cgcggcgcgg caggccagcg
     1441 gattgccggt ataggaatgc gaatgcagga aaccgcgcgc ggtctcgtcg gcgtagaacg
     1501 cccgataaat ctcgtcggtg gtcatcgcga tcgacagcgg cagataaccg ccggtgatgc
     1561 ccttggagat cagcagaaag tcgggggtca taccctctcc cccaacccct ctcccgtaaa
     1621 cgggcgaggg gagcacgtcg ctccccttat cccctcgccc ctccggggag agggttaggg
     1681 agaggggctt tgcctgctcg caggcgaaca tcgtgccggt gcggcccata cccacggcga
     1741 tctcgtcggc gatcaggtgc acgccatatc gcgtgcagag ttcgcgcgca cgttgcagat
     1801 aagccgggtg atacatcgcc atgcccgccg cgccctgcac cagcggttcg atgatcagtg
     1861 cggcgagttc tgaatggtgc tgctgcagat gggcttccag cgcatcggcg cagcgcagcg
     1921 cgtactgttc ggcgctctca cccggcgcgg cacagcgcca gtccgggctg ggcacggtgg
     1981 cctgctgcgc gatcagcgcg ccgtaggcat cgcgaaacaa cgccacgtcg gtcaccgaca
     2041 gcgcgcccag cgtctcgccg tggtagctgt tctgcaggct gatgaaccgg gccttgccgg
     2101 gctgtccgct gttgcgccag tagtgagcac tcatcttcag cgcgatctcg gtggccgatg
     2161 ccccgtccga accgtagaag cagtggccca atccggcagg cgcgagttcg cgcaggcgct
     2221 cggacaactg caccaccggc tcgtgggtga agcccgccag catcacatgc tccagccggc
     2281 ccagctgatc gaccagcgcc gcgttgatgc gcggattgca atgaccgaac aggttcaccc
     2341 accaggagct gacgccgtcc agatagcggt tcccgtcgaa atcatggagc cagaccccct
     2401 gcccgcgcgc gatcggcacc aacggaaagc tctcgtagtg cttcatctgc gaacagggat
     2461 gccagacggc ggaaacgctg cgcgacaata gggtggcgtt gctggaagtg cgatgcgaca
     2521 tgcgcgaaat cataacgttt tttgcgcctt gtcagcggga gaatgtttgg atagcctgac
     2581 aggcgtcaca accaagggag gcgaatcatg cacatcctca tcacgggggg caccggcttg
     2641 atcggcaggc ggctctgcca agcgctgctc gccgaaggcc acgaactgac ggtgctcagc
     2701 cgcaagccgg attccgtcgc gggcaagtgc ggcgccaggg tgcatgcgat ggcctcgctg
     2761 catgactggc atccttctca aaccttcgat gcggtgatca acctggccgg cgagcccatc
     2821 gtcgacgaac gctggagcgc caaacgcaag cagctcctgt gggacagccg ggtcacgctg
     2881 accgagaatc tggtgaggcg gctcgccgcc gccgaacgca agcccaccgt gctgctgagc
     2941 ggatcggcgg taggttatta cggcaatggc ggcgaccgca tgctggacga atcgtccgac
     3001 gcgggcgaag gctatgcggc agagctatgc aaggcctggg aggatgccgc acgcgtcgcc
     3061 gaaagcaccg gcgtgcgcgt ctgcctgctg cgcaccgggc tggtgctgag ccgtgacggc
     3121 gggctgctgg aacgcatggt cccgccattc aagctgggct tgggtacccg cgtcggcaat
     3181 ggcaaacaat ggatgagctg ggtacatatc gaagactgca tcgccatcct gctgtgcctg
     3241 ctgcacgacg aacagatgcg cggcccttac aacgtcaccg cgccgcaacc ggtcagcaat
     3301 gccgatttca ccaaagtcct ggcgggcgtg ctgcgccgcc ccgccccctt cgccgctccc
     3361 gccgcgctgt tgaacatgag catgggcgaa tctgccagcc tgatactgga agggcagcgc
     3421 gtgctgccgc gcaaactgga agtcgcgcac taccgcttcc agttcagtgc attgcgcgag
     3481 gcccttgagg atttactcga ccaaaccccg gctcgctgac agcccgcatc agccctgctt
     3541 gctttccgtc ccccccaatc tgaggcatac ttcgcacctt gagtaattat tatctctgcc
     3601 agttcaacct gaggttgacc atggcagttg gaggatggca gtaatgaaac gtgtggacga
     3661 tttccgtctg cgctttggca agcgcgagat agtaccgatc atgatcggcg gtatgggggt
     3721 ggacatctcc acggccgaac tggcattgga ggccgcacgt ctgggtggta tcgggcatat
     3781 ttcggacgcg atgctgccga ccgtgaccga ccgcctctac gacaccagct tcgtcaagga
     3841 aaagctccag cagtacaaat acaacatcga caacgccgac aagtcggcgg tcaagttcga
     3901 cctcggccat gtcgccgaat cgacccgcct gcatgtgagc aagaccatgg aagccaagcg
     3961 tggcgagggc atggtgttcc tcaactgcat ggaaaaactg accatgaacg cgccgcgcga
     4021 gaccctgacg gtgcgcctgc gcagcgcgat ggacgcgggc atcgacggca tcacactggc
     4081 cgccgggctg catctgggct cgttcgcgat gatcgaggat cacccgcgct tccgcgacgt
     4141 caagctgggc atcatcgtct cgtccttgcg cgcactgcaa ctgttcctgc gcaagaatgc
     4201 ccgcaccaac cgcctgcccg actacatcgt ggtggaaggg ccgctggcag gcggccatct
     4261 gggcttcggc ctggaagact ggatgaagta tgacctgcgc accatcacca acgaagtgct
     4321 gcaatatctg cgcgacgaaa agatcgacat tccggtcatt ccggctggcg gcatcttcac
     4381 cggcagcgat gcggtgagct tcctggaaga aggctccgcc gccgtccagg tcgcgacccg
     4441 cttcaccgta gccgaggaat gcggtctgcc gaaagacgtg cagcaggaat acttcaaggc
     4501 cagcgagcac gatatcgagg tcaacggcgt ttcgccgacc ggctatccga tgcgcatgct
     4561 gaagaacagt ccggccatcg gctccggcat ccgtccgggc tgcgaatcct acggctatat
     4621 tctggatgcc aacggcaagt gctcctatat cgattcctac aaccgcgagg tcgcgctgca
     4681 ccccggcgag aagaaaatat cggtcatgga caagacctgt ctgtgtaccc acatgcgcaa
     4741 cttcaagctg tggacctgcg gccactacac ctacaagctg aaagacacca cccacaaact
     4801 ggccgatggc agctatcagg tcctgtctgc ggaacacatc ttccacgact accagttcag
     4861 caccgacaat caggtcgccc tgcccccgca aaaataatca tgcgccccgc ccttgaaatt
     4921 gggcggggcc gcccctatta ttagcagtcg ctg
//
LOCUS       DASUIV010000026         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_750121, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000026 DASUIV010000000
VERSION     DASUIV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750121"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFW59_00850"
     CDS             <1..211
                     /locus_tag="VFW59_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6781 family protein"
                     /protein_id="HEX5362795.1"
                     /translation="LKHSGTRTGEQVREALKQMASGVQASSEVGRAGLSDAASTATER
                     LSHVASGILEALSDSLKRQSERLRS"
     gene            295..1974
                     /locus_tag="VFW59_00855"
     CDS             295..1974
                     /locus_tag="VFW59_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5362796.1"
                     /translation="MLWETLSVVRDLPRLHEIASVMIRYGGGDLVRALGISGALERAG
                     RVLHWQSTSEISQLEAPVRIRRALEELGPTFVKLGQLLATRVDMFPPHWIAEFEKLHS
                     QVPAVSYESLHPDLVAAIKGEPTEVFAEFNVVPLAAASIAQVHRATLKDGTPVVVKIR
                     RPGIEGVIRADLRILEHAAKLLESEVPDFRRYDPSQIVSQFRRSLNRELDLAKEARNI
                     DQFARDFADDPLVKIPLVYWEFTNDRVNVQEEIVGLAGVAPDKLRARGLDPKLLAARG
                     ADTVLRMVLEHGYFHADPHPGNILFLPENRIGVIDFGMVGMLTNPRRNQIVDLLHALV
                     RKDEQALLQVLLDWSGDSVLDEDRLAYDVTELLQSYDDLQLKDVKIGALLNDITALMR
                     DNNLLLPADLTLLFKTLITLEGLGQQLDPEFHMIDHVTPFVERVIQQRYTPQALLARG
                     RKSVRETLEVLADLPRDLRHLLRDVRRGRVKVDLDLKRLDQFGHQLNRASNRLTMGIL
                     TASLVVGSSIIMTVKGGPQLFGLPFFGLLGFLIAFFNSLWIIFSIWRSGKH"
     gene            complement(1984..>2176)
                     /locus_tag="VFW59_00860"
     CDS             complement(1984..>2176)
                     /locus_tag="VFW59_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5362797.1"
                     /translation="FARMMSDAGYSLVAEGVETDTILRKVESLGIAHVQGYLLGRPMS
                     LADVAASLATRDSGVTCAV"
BASE COUNT          412 a          689 c          669 g          406 t
ORIGIN      
        1 cttgaaacac agtggcactc gcaccggcga acaggtgcgc gaggcgctca agcagatggc
       61 gagtggcgtg caggccagca gcgaggtggg gcgcgcgggc ttgagcgatg ccgccagtac
      121 agccaccgag cggctgtcgc acgtcgcaag tggcattctg gaagccttgt ccgactcgct
      181 caagcgacag agcgaacgcc tgcgctcctg acgcctctcc aattggcagc gggaattcac
      241 ccgctgcgcc gcttgcggct aaactctcag cattattgat taaaaaaacc aacgatgctc
      301 tgggaaaccc tttcggtcgt gcgcgacctg ccgcgtttgc atgaaatcgc ctcggtgatg
      361 attcgttatg gcgggggcga cctggttcgc gccctcggca tcagcggcgc gctcgaacgc
      421 gccgggcggg tgctgcactg gcagagcacc agcgagatca gccagctcga ggcacccgta
      481 cgcatccgcc gcgcgctgga agagctgggg ccgaccttcg tcaagctggg tcaactgctg
      541 gcgacgcggg tcgacatgtt tccgccgcac tggattgcgg aattcgagaa gctgcacagc
      601 caggttcctg cggtctccta tgaaagtctg caccccgatc tggtcgccgc catcaagggc
      661 gaacccaccg aggtgtttgc cgaatttaac gtcgtgccgc tggccgcggc atccatcgcg
      721 caggttcacc gcgcaaccct caaggacggt acgccggtcg tcgtcaaaat ccgccgcccg
      781 ggtatcgagg gcgtgatccg ggccgacctg cgcatcctgg aacacgccgc caagttgctg
      841 gaaagcgaag tgccggactt ccggcgttac gacccgtcgc aaatcgtctc gcagttccgc
      901 cgctccctga atcgtgaact cgatctggcc aaggaggcgc gcaacatcga tcagttcgcg
      961 cgcgattttg ccgacgatcc actggtgaag attcctctgg tgtactggga attcaccaat
     1021 gaccgcgtca acgtgcagga agaaatcgtc ggcttggcag gcgttgctcc cgacaagctg
     1081 cgtgcccggg gactcgatcc caagctgctt gctgcacgcg gcgccgacac ggtgctgcgc
     1141 atggtgctgg aacacggcta ttttcatgcc gatccccacc ccggcaatat cctgttcctg
     1201 cccgaaaacc gcattggcgt gatcgacttc ggtatggtgg ggatgctcac caatccccgc
     1261 cgcaatcaga tcgttgacct gctgcacgcc ctggtgcgca aggacgaaca agcgctgctg
     1321 caggttctgc tcgactggag cggcgattcg gtgctggatg aggaccgcct ggcttacgac
     1381 gtgacggaac ttctgcaaag ctacgacgac ctgcagttga aggacgtgaa aattggcgcc
     1441 ctgctgaacg acatcaccgc cttgatgcgc gacaacaatc tgttgctgcc ggccgatctc
     1501 accctgttgt tcaagacgct tatcacgctg gaaggactgg ggcagcagct cgaccccgaa
     1561 ttccacatga tcgaccacgt gacgccattc gtggaacgcg tcatccagca gcgttacacg
     1621 ccgcaggcgt tgctcgcgcg cgggcgcaag agcgtgcgcg agacgctgga ggtgctggcc
     1681 gacctgccgc gcgacctgcg gcatctattg cgggatgtgc ggcggggccg ggtgaaggtc
     1741 gatctcgacc tgaaacggct ggaccagttc ggccatcagc tgaaccgtgc aagcaaccgg
     1801 ctgaccatgg gcatcctgac agcctcgctg gtcgtcggct ccagcatcat catgacggtg
     1861 aaaggcggcc cccagctatt cggcctgcca tttttcggct tgctgggatt cctgatcgcg
     1921 ttcttcaaca gcctgtggat catcttttcc atctggcgtt ctggcaagca ctgatgcccc
     1981 ggatcaaacg gcgcaggtga cgccgctatc ccgggtggcg aggctggcag ccacgtccgc
     2041 cagcgacatg ggccggccca gcaaataacc ctgcacatgg gcaatgccga ggctttcgac
     2101 cttgcgcaga atggtgtcgg tttccacacc ctcggccacc agtgaatagc ctgcgtcgct
     2161 catcatgcgg gcgaag
//
LOCUS       DASUIV010000027         5532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1215545, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000027 DASUIV010000000
VERSION     DASUIV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215545"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VFW59_00865"
     CDS             complement(<1..772)
                     /locus_tag="VFW59_00865"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5362798.1"
                     /translation="MTSHNQQLFDESQKIIPGGVNSPVRAFKSVGGTPLFFKRGKGAY
                     VWDADDKQYIDYVNSWGPMIVGHCHPEVVKAVQAAAAEGLGFGAPTASELEIAELLCK
                     ILPSLEMVRLVSSGTEATMTAIRLARGFTGRSRIIKFEGCYHGHSDGLLVKAGSGALT
                     FGQPSSSGVPAEIAALTTVLDYNDAAGLEKAFAEYGDEIAAVIVEPVAGNMNLILPKR
                     EFLDAMRKLCTKHGAVLILDEVMSGFRVGLQGAHGHFGI"
     gene            complement(769..1416)
                     /gene="thiE"
                     /locus_tag="VFW59_00870"
     CDS             complement(769..1416)
                     /gene="thiE"
                     /locus_tag="VFW59_00870"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5362799.1"
                     /translation="MVRTFQQTISGLYAITPDERDTAELLRKVRLALQGGVRVLQYRN
                     KLADASLRAEQAGALSKLTREFSVPLIVNDDAELARQAGADGVHLGGTDGSVAAARAL
                     LGSGSIIGVSCYNRLALAQDAVSQGADYVAFGSFFASTVKPDAVAATPDLLRHARAGL
                     TVPLVAIGGITVQNGTQLLEAGANALAVISAVFAAPDIAGAATDFSNLFEQGNQA"
     gene            complement(1509..2348)
                     /locus_tag="VFW59_00875"
     CDS             complement(1509..2348)
                     /locus_tag="VFW59_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEX5362800.1"
                     /translation="MSESFPLVMTFSATDPSGGAGLQADLLTISSMGCHPLSVVTAIT
                     VQDTSGVDDVLPIDPEWVVDQARAMLEDVPVAAFKIGLLGSVENIAAIAEVMADYPDI
                     PLVLDPVLASGRGDELTNDDMLDAMRELLIPQTTIITPNSIEARRLALDEDNEEDDPS
                     LEECAKRILRMGCEYVLITGTHEQTSKVVNTLYNERGVVRSDSWARLPGIYHGSGCTL
                     ASAIAALLAQDVSVPDAVKEAQEYTWQTLSAGFRPGMGQHIPDRLFWARGEEDEAQSE
                     RPN"
     gene            2446..2826
                     /gene="gloA"
                     /locus_tag="VFW59_00880"
     CDS             2446..2826
                     /gene="gloA"
                     /locus_tag="VFW59_00880"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX5362801.1"
                     /translation="MRILHTMLRVGDLEKSLAFYTAVLGMKLLRRTDYPEGKFTLAFV
                     GYGDEAHGAVIELTHNWGVDKYEIGNAFGHIAIEVEDAYATCDAIKSRGGKVVREAGP
                     MTHGSTVIAFVEDPDGYKIELIQR"
     gene            complement(2872..3324)
                     /locus_tag="VFW59_00885"
     CDS             complement(2872..3324)
                     /locus_tag="VFW59_00885"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX5362802.1"
                     /translation="MKKILAVFFIAMLAVSLVACQTKQQGGGVVGGVLGGVLGNQVGG
                     GSGRTAAIIAGTILGAYLGSEMGRYMDENDTRKAESAMEYNRDHQASTWHNPNTGAEV
                     TAMPTRTFKSADGENCREYQTTVTVSGKKEKAYGTACRQPDGSWKIVN"
     gene            3546..4796
                     /locus_tag="VFW59_00890"
     CDS             3546..4796
                     /locus_tag="VFW59_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5362803.1"
                     /translation="MTSSSKSILSIAILSALLTACGGGDKPAAGPGGPGAAMPPPEVD
                     VITVGTGSATLTQDLPGRLQAYRTAQVRARVEGVIEKRHFQEGSEVKAGASLFQIDAR
                     TYRAAAEATKADYAIARQTVERYKPLLEIKAVSQQEYDLASAKAKQAEAAMTRAEEDL
                     ENASVPAAISGHIGRALVTEGAFVGKGEATLLATIEQLDPIYANFTQSGGDLLRLQQA
                     IKAGKLKQSESAKVELLLEDGSVYPLPGKLFFTDMAVDPSTGSVSLRAEFPNPQRELL
                     PGMFVRIRFPQAVAENAIRVPQRAVQSGPQGQFVMVVGAENKATPVPVKTGGMAGTDF
                     VIADGLKGGEQVIVNGLQKARPGTPVKAVPWDGKAPQIAMPPAAKPADQKPSDQKPSE
                     QQSGDQKSSDQKSSDPKPAAPKRQ"
     gene            4809..>5532
                     /locus_tag="VFW59_00895"
     CDS             4809..>5532
                     /locus_tag="VFW59_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5362804.1"
                     /translation="MFAKFFIDRPIFAWVIALIILLGGALALKNLPVAQYPAVAPPAL
                     TVTVNYPGASAQVLEETAVALLEQEMNGIDNLLYMESASELGTGSITLTFVAGTNLDQ
                     ASVEAQNRIKRVEARLPEEVRRLGVTVAKTARNYVMFVALFSPDKSLNDVALGSYASA
                     NVVDSLRRVPGVGEVLLFGTEYSMRLWLKPEKLQNYNLSPADVAKAVRAQNSQLATGE
                     LGQLPSAAGQQLNAVIVTRSRLS"
BASE COUNT         1051 a         1790 c         1658 g         1033 t
ORIGIN      
        1 tgatgccgaa atgtccatgc gcgccctgca agccgacgcg gaaacccgac atcacctcgt
       61 ccagaatcag caccgcaccg tgtttggtgc agagcttgcg catcgcatcg aggaactcgc
      121 gcttcggcag gatcaaattc atgttgccgg ccacaggctc gacgatcacc gcagcgatct
      181 cgtcgccgta ttcggcaaag gctttttcca gaccggccgc atcgttgtaa tccagcaccg
      241 tagtcagcgc ggcgatctcg gcgggcacgc cggaagaact cggctggccg aacgtcagcg
      301 cgccggaacc cgccttgacc agcagaccgt cggaatgacc gtggtaacaa ccctcgaatt
      361 tgatgatgcg cgaacgtccg gtgaagccgc gcgccagacg gatcgcggtc atcgtcgctt
      421 cggtgccgga gctgaccaga cgcaccatct ccagcgacgg cagaatcttg cacagcagct
      481 ccgcaatttc cagctcgctc gcggtcggcg cgccgaagcc cagaccttcg gctgctgctg
      541 cctgcaccgc cttgaccacc tcgggatggc agtgaccgac gatcatcggc ccccaggaat
      601 tcacatagtc gatgtactgc ttgtcgtccg catcccacac ataggcgccc ttgccgcgct
      661 tgaagaacaa cggcgtgccg cccaccgact tgaacgcgcg caccggcgaa ttcacaccgc
      721 cggggatgat tttctgggat tcgtcgaata gttgctggtt gtgcgaagtc atgcttgatt
      781 accttgctcg aataaattgg aaaaatctgt tgctgcgcct gctatgtccg gtgccgcgaa
      841 tactgccgag ataaccgcca gcgcgttcgc gcccgcctcc agcaactgcg tgccgttctg
      901 cacggtgatg ccgcctatcg cgaccagcgg cacggtcaaa ccggcgcgtg cgtgccgcaa
      961 caagtcgggc gtagcggcca ccgcgtccgg ctttacagtc gaggcgaaga agctgccgaa
     1021 cgccacgtag tccgcccctt ggcttaccgc atcctgcgcc aaagccaagc gattataaca
     1081 agacacgccg atgatgctcc cgctgcccag caatgcgcgc gccgctgcga cgctgccgtc
     1141 cgtcccgccc agatgcacgc cgtccgcgcc tgcttgtctg gccagttccg catcgtcgtt
     1201 gacgatcagc ggcacggaaa attcgcgggt cagcttgctc aacgcaccgg cctgctccgc
     1261 gcgcaatgac gcgtcggcca gcttgttgcg gtactgcaac acccgcacac cgccttgcaa
     1321 ggccagccgc accttgcgca gcaactccgc cgtatcccgc tcgtccggcg tgattgcgta
     1381 taaaccgcta atggtctgtt ggaaggttcg taccacgtat ctgcttgcta ccctctcctc
     1441 aatcctctcc cgcatgcggg agaggaagcc aacgtccgcc ctaaggggcg ggttatttga
     1501 tcctgcctct aattcgggcg ctcagactgc gcctcatctt cttcgccgcg cgcccagaac
     1561 aatcggtccg gaatgtgctg tcccatgccg gggcggaatc ccgcgctcag tgtctgccat
     1621 gtgtattcct gcgcctcctt gaccgcatcg ggcacggaca cgtcttgcgc cagcaatgcg
     1681 gcaatcgcgg aggccagcgt gcagccggag ccgtgataga tgcccggcag gcgcgcccaa
     1741 ctgtcgctgc gcaccacgcc gcgctcgttg tacaaggtgt tgacgacctt ggaggtttgc
     1801 tcgtgcgtgc cggtaatcag tacatattcg caacccatgc gcaggatgcg tttggcgcat
     1861 tcctccaggc tcggatcgtc ttcctcgttg tcttcgtcca gcgcgagacg gcgcgcttcg
     1921 atgctgttcg gcgtgatgat ggtggtctgc ggaatcagca attcgcgcat cgcatccagc
     1981 atatcgtcgt tcgtcagttc gtcgccgcgc ccagatgcca gcaccgggtc gagcaccagc
     2041 ggaatgtccg gataatccgc catcacttcg gcaattgccg cgatattttc cacgctgccc
     2101 agcaagccga tcttgaatgc cgcgaccggc acgtcttcga gcatcgcgcg cgcctgatcc
     2161 accacccact ccggatcgat cggcagcaca tcgtccaccc cgctggtgtc ctgcacggtg
     2221 atcgccgtca ctaccgacag cggatgacac cccatgctgg agatagtcag cagatcggct
     2281 tgcaggcccg cgccgccact ggggtcggtt gcggaaaaag tcataactaa cgggaacgat
     2341 tcggacatga tgtgggcaat ggtaatatgc gcggctaatc gggcatttta ccctatatgt
     2401 ccgcgactac cgaacccaac tgcaattccc gacagagagg acccgatgcg cattctgcac
     2461 accatgctcc gcgtaggcga tctggaaaaa tcgctcgcct tctacaccgc cgtgctcggc
     2521 atgaaattgt tgcgccgcac cgactacccg gaaggcaagt tcacgctggc ctttgtcggc
     2581 tatggcgacg aggcgcatgg cgcggtgatc gagctgacgc acaactgggg cgtggacaag
     2641 tacgagattg gcaacgcctt cggccacatc gccatcgaag tggaagatgc ctatgccacc
     2701 tgcgatgcga tcaagtcgcg cggcggcaag gtggtgcgcg aagccgggcc gatgacgcac
     2761 ggcagcacgg taatcgcgtt tgtcgaagac ccggacggct acaagatcga gctgatccag
     2821 agatagttgt tttccgcttg gcggcgaaat gccgcatgcg tccgcactcc atcagttgac
     2881 gatcttccat gaaccgtccg gttggcggca ggcggttcca taggcctttt ccttcttgcc
     2941 gctgacggtg acggtggtct ggtattcgcg gcagttctcg ccgtcggcgg atttgaaggt
     3001 gcgggtcggc attgcggtaa cctccgcgcc ggtattgggg ttgtgccagg tactcgcctg
     3061 atggtcgcgg ttgtactcca tcgccgactc ggccttgcgc gtatcgtttt cgtccatgta
     3121 gcgccccatc tcgctgccca gatatgcgcc cagtatcgtt ccggcaatga tcgcggcggt
     3181 tcggcccgac cctccgccca cctggttccc cagcaccccg cccaacacgc cgcccacgac
     3241 cccgcctccc tgttgcttgg tctggcaagc aaccagcgac accgcaagca ttgcgatgaa
     3301 aaacacggcc agaatttttt tcatgatggc atctcctgta tatgaacgtc gtccggcccc
     3361 gagcatagag aatctgcgct cggcaataca tacagggaaa cctgcatttg tccggaatat
     3421 gcacctgcct tgcggcgcgg ttggcctgta tccggattgc atttcaaaca agcgtttgat
     3481 acgctcgttt ttttgcgccg catgccatca ccgggcatcc cttaaacaaa gcaggatcat
     3541 tcaccatgac ttcttcgagc aaatccatcc tttcaatcgc gatcctgtcc gcgctgctga
     3601 cagcttgtgg cggcggcgat aaaccggcag cggggcccgg cggcccgggc gcggcgatgc
     3661 cgccgcccga ggtggatgtg attacggtcg ggaccggcag cgcgacgctg acgcaggatt
     3721 tgccgggccg tttgcaggct taccgcaccg cgcaggtgcg cgcgcgggtc gaaggcgtga
     3781 ttgaaaagcg gcactttcag gaaggcagcg aagtgaaggc tggcgcatcg ctgttccaga
     3841 tcgacgcgcg cacttaccgc gctgcggctg aggctaccaa ggccgattac gcgatcgcgc
     3901 gccagacggt cgagcgctac aagccgctgc tcgaaatcaa ggcggtcagc cagcaggagt
     3961 acgatctcgc cagcgccaaa gccaagcagg ccgaggccgc gatgacgcgc gccgaggaag
     4021 acctggagaa cgccagcgtc cccgccgcga tctcgggcca catcggccgc gcgctggtga
     4081 ccgaaggcgc attcgtcggc aagggtgaag cgacgctgct ggcgaccatc gagcagcttg
     4141 atccgatcta cgccaatttc acgcagtcgg gcggcgatct gctgcgcctg caacaggcca
     4201 tcaaggcggg caagctgaag caatctgaat cggccaaggt cgaattgctg ctggaagacg
     4261 gcagcgtcta tccgttgccg ggcaagctgt tcttcaccga catggcggtc gatccgagca
     4321 ccgggtcggt gtcgctgcgc gccgaatttc ccaatcccca gcgcgagctg ctgccgggca
     4381 tgtttgtgcg catccgcttc ccgcaggccg tggctgaaaa cgcgatacgc gtgccgcaac
     4441 gcgccgtgca atccggcccg caggggcagt ttgtgatggt ggtgggcgcg gagaacaagg
     4501 ccacgcccgt gccggtgaaa accggcggca tggcgggcac tgacttcgtg attgccgacg
     4561 gcctcaaggg cggcgaacag gtgatcgtga acggcttgca gaaagcgcgt cccggcacgc
     4621 cggtcaaggc cgtgccgtgg gacggcaagg ccccgcagat cgcgatgccc ccggcggcaa
     4681 aaccagccga ccaaaagcca tctgaccaga agccatctga acagcaatct ggcgaccaga
     4741 agtcatctga ccagaaatca tctgacccga aacccgctgc cccaaaacgc cagtaaataa
     4801 ggcccattat gttcgctaaa ttttttattg atcggcccat cttcgcgtgg gtgatcgcgc
     4861 tcatcatcct gttgggcggc gcactggcat tgaaaaacct gccggttgcg cagtacccgg
     4921 cggttgcgcc gcccgcgctg accgttaccg tcaactatcc cggcgcttcg gcgcaggtgc
     4981 tcgaagaaac ggcggtcgcg ctgctcgagc aggaaatgaa cggcatcgac aatctgctct
     5041 acatggagtc cgcctccgag ctgggcaccg gcagcatcac gctgaccttt gtcgcgggca
     5101 cgaacctcga tcaggcctcg gtcgaggcgc agaaccgcat caagcgcgtc gaggcgcgtt
     5161 tgccggaaga ggtgcgccgc ctgggtgtga ccgtcgcgaa gaccgcgcgc aactacgtga
     5221 tgttcgtcgc gctgttctcg cccgacaaga gcctgaacga tgtggcgctg ggcagctatg
     5281 ccagcgccaa cgtggtagac agtttgcggc gcgtgccggg cgtgggcgag gtgctgttgt
     5341 tcggcaccga atactcgatg cgcctgtggc tcaagccgga aaagctgcaa aactataatc
     5401 tgtccccggc ggatgtcgcc aaggcggtgc gggcgcagaa ctcgcagctc gccaccggcg
     5461 aactcggcca gttgccgtcg gcggcggggc agcagctcaa cgcggtgatc gtcacccgca
     5521 gcaggctttc ca
//
LOCUS       DASUIV010000028         4754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_936451, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000028 DASUIV010000000
VERSION     DASUIV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936451"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFW59_00900"
     CDS             <1..807
                     /locus_tag="VFW59_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362805.1"
                     /translation="RTAGYHGPNIFSAAALKLIASASNGLMRRVNILADKSLLAAFVE
                     DTHNIEVRHVLAAMRDSAIKPPRTLPGKPLLWAGGTAAALLAGLTTWQLLKQTPAPAV
                     ALPTPQANVQADVQVAEVAAPPAATQPPITEPTAAPPARETIAAVPPPAQPAPEHPAP
                     ADPAPPRSSLFAQRLAAGQQLLAKRQHAASIQLFYSEENQPGKIEGFLKRAEGLGKLS
                     DIYLLPAKSGSKEGLRVLYGAYPSADAARNAIKDLPTRYQEAFATSIYIF"
     gene            978..1247
                     /locus_tag="VFW59_00905"
     CDS             978..1247
                     /locus_tag="VFW59_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEX5362806.1"
                     /translation="MPTISMFYGIVICLYFYDDERHKLPHIHARYQGQEASFSILDGA
                     RLSGEIPEAKARLVQAWIEIHRESLLADWELAVNGQTPFAIDPLR"
     gene            1256..1510
                     /locus_tag="VFW59_00910"
     CDS             1256..1510
                     /locus_tag="VFW59_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX5362807.1"
                     /translation="MESVVRVVPRNDFSLELWFDTGDHRLFDARPYLNRGVFTRLQDI
                     ALFKQAFIALDTVCWPGDLDIAPETLYDRSVPLDEPSHAA"
     gene            1629..3407
                     /locus_tag="VFW59_00915"
     CDS             1629..3407
                     /locus_tag="VFW59_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX5362808.1"
                     /translation="MWCSRNILHLCVIAATLAGCGTRPVQPSDKHILQQQDQPSAATA
                     IPQPLKHSITLPPPGPAAKVETYSVVVTNVDAQEILFALARDAKINLDIHPGIQGSVT
                     LNALDQTLPQILTRIAKQIDMRYELDNGNLIVMPDTPYLHSYKIDYVNMTRDSEGTMS
                     NVTQVGSGSQGNTAVAAGTGSNNSSISIKGTSKNRFWETLTRNVKDILHETDKILPEG
                     SSETSVQQVRAASSTGTGAQSATNRKATPRGGIENSPNPVNIEEGGMTVTRRSTFREA
                     AAVIDNPETGIITVRATARQHEKIQEFITQVMNSARRQVMIEATIVEVNLSDNYKQGI
                     NWSALIKAGGKQFKLVQSSAGLPTGVGNGMLGIITNPTAATITQQVSLLESFGTVKVL
                     SSPKLSVMNNQTAILRVVDNLVYFVINVTTTAPTATSSAFSTFATTPNTVPIGFTMSV
                     TPQINDSDTVQINLHPSISRLIGYVNDPNPSLATAGVVSRIPVTSTREMESILKIENN
                     QIAVMGGLMQDSINNLTDAVPGLSGIPLAGELFKQRNDTSTKTELVIFLRPVVIKDAS
                     IAGDFSAYRNTLPDRNFFQDPAHAQP"
     gene            3420..4448
                     /locus_tag="VFW59_00920"
     CDS             3420..4448
                     /locus_tag="VFW59_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5362809.1"
                     /translation="MSLLLDARKKSQLALSAQGDERIQTAPDNNNRSAGQNLFNAKSP
                     LNRIAGFGMQRNLLLALGGTALLLVGGAVYLWHLGSSSSTRPLQPNTASQPVAASPQP
                     ASAATPPAPVAALAAPADTKTRAPISPAAIAPTESAPLPLAPARKAIRIERQSAERID
                     PAPDNAYRAYRDGKLDEAQQLYRELLGKDAHNTGALLGLAAIAQQLGEETLAAQYYAD
                     VLRLDPRNATANAGMAALNADENSESRLKTLLGEQPDSAALHFALGNLYAGQARWGEA
                     QQAYFNASALEPDNAEFAFNLAVSLDHLGQSKLAAQQYRRALQLDGQNRAGFDHAQTE
                     QRLQALTR"
     gene            4455..>4754
                     /locus_tag="VFW59_00925"
     CDS             4455..>4754
                     /locus_tag="VFW59_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system protein E"
                     /protein_id="HEX5362810.1"
                     /translation="MPDSLQQPLGRILVDKGVISDDQLRIALQEQNKTHQPLGRLLVR
                     LGFLSEATIRDVLSENLGQESVDLSAVIIDPAALALIPKDVARRYQLLPLSLDPHS"
BASE COUNT         1056 a         1592 c         1344 g          762 t
ORIGIN      
        1 cgcacggctg gctatcatgg tccgaacatc ttctctgctg cggcgctcaa gctgatcgca
       61 agcgcctcca acggcctgat gcggcgcgtc aacattcttg ccgacaaatc gttgctggcc
      121 gcgtttgtcg aggacaccca caacatcgag gtgcgacatg tcctggcggc gatgcgggac
      181 agtgcgatca agccgccccg cacgctgccc ggcaagccgc tgctgtgggc gggtggcaca
      241 gcagccgcgc tgctggcggg actgacgacc tggcaactgc tgaagcaaac gccagcgcca
      301 gcggtcgccc tgccgacgcc ccaagcaaac gtacaggcag acgtacaggt ggcggaagtc
      361 gccgcgccgc ccgccgccac ccaaccgccc ataaccgagc cgaccgccgc tccgcctgcg
      421 cgtgaaacca tcgctgccgt accgcccccg gcgcagcctg cccctgagca ccccgcgcca
      481 gccgacccgg ccccgcccag aagcagcctg ttcgctcaac gtctcgccgc aggtcaacag
      541 ttgctcgcga aacgccagca cgcggccagc attcaactgt tctacagcga ggaaaaccag
      601 cccggcaaga tcgagggatt cctgaaacgc gcggaaggac tcggcaagct gtcggacatt
      661 tacctgctgc ccgccaagtc cggcagcaaa gaaggcctgc gcgtgctgta tggcgcctat
      721 ccatcggccg atgcggcacg caatgcaatc aaggacctgc caacgcgcta tcaggaagcc
      781 ttcgccactt ccatctacat cttctgactg ccgacaagtc aaaagtagtt gactctcgat
      841 tttcaggagc ccgcaaggaa acttgagagg gggcgttgaa atgtatccat gcggtaggta
      901 atggagtggc agagcgctat tgatttaaat agttgttgat tacaattgcg caacgccgtc
      961 agaaacagag gtttgccatg ccaaccatca gtatgtttta tggaattgtt atctgtctgt
     1021 atttttacga cgatgagcgc cataaactgc cccacattca tgcaaggtat caagggcaag
     1081 aggcatcgtt ctcaatcctt gatggggcgc gactcagtgg ggaaatccct gaggctaagg
     1141 caaggctggt acaggcgtgg attgaaatac atcgagagtc gttgttggcc gattgggagt
     1201 tggctgttaa tggtcaaact ccttttgcaa tcgatccgtt gcgttaggag ggaatgtgga
     1261 atccgttgtc cgtgttgtgc cgcgaaatga tttttcgctg gagttgtggt tcgataccgg
     1321 ggatcaccgt ttgtttgatg ctcgccctta cctgaatcgc ggcgttttta cccgcctgca
     1381 ggatattgcc ttgttcaagc aggccttcat cgctctggat acagtgtgct ggccgggcga
     1441 tctggatatt gctcccgaaa cactctacga tcgctcagtg ccgcttgacg aacccagcca
     1501 cgccgcgtga tcgggcgggt tgtagttgca cccaatatcc tgaaatgatt tccggcaaga
     1561 ttgccgccaa gtattgagcg gggcacgcaa agctgctagt ctcccgacac aaacgtgggg
     1621 ggaaggcaat gtggtgttca cgcaacattc tgcatctctg tgttatcgcc gcaacgctgg
     1681 ccggttgcgg cacccggcct gtccaaccgt cggacaagca catcctgcaa cagcaggacc
     1741 aaccctccgc cgcaaccgcc atcccgcaac cgctcaaaca cagcatcacg ctgccgccgc
     1801 ccggccccgc agccaaagtt gaaacctaca gcgtcgtcgt caccaatgta gacgcgcagg
     1861 aaatcctgtt cgcgctggcg cgcgacgcga aaatcaatct ggatattcat ccgggcatcc
     1921 agggaagcgt cacgctgaac gcgctcgacc agaccctgcc gcaaatcctc acgcgcatcg
     1981 ccaaacaaat cgacatgcgc tatgagctgg acaacggcaa cctgatcgtg atgcccgaca
     2041 ccccttacct gcacagctac aagatcgact acgtgaacat gacgcgcgac tcggaaggga
     2101 cgatgtccaa cgtcacgcag gtcggctccg gctcgcaggg caacacggcc gtcgcggccg
     2161 gcaccggcag caacaactca tccatcagca tcaagggcac atcgaagaac cgcttctggg
     2221 aaacgctgac ccggaacgtc aaggacatcc tgcacgagac ggacaaaatc ctgccggagg
     2281 gttccagcga aacctccgtt cagcaagtcc gggccgccag cagcaccggg acgggcgcgc
     2341 agtccgcgac caaccggaag gcgacgccga ggggcggcat cgaaaacagc cccaatccgg
     2401 tcaatatcga agaaggcggc atgaccgtca cgcggcgcag cacgttccga gaagccgcag
     2461 cggtcatcga caacccggaa accggcatca tcacggtgag agccaccgcg cgccagcacg
     2521 aaaaaatcca ggaattcatc acgcaggtaa tgaacagcgc gcgccgccaa gtgatgatag
     2581 aggcgaccat cgtcgaggtg aacctgagcg acaactacaa gcagggcatc aactggtcgg
     2641 cgctcatcaa ggcgggcggc aagcaattca agctggtgca aagcagcgcc ggcttgccca
     2701 ccggcgtcgg caacggcatg ctcggcatca tcaccaaccc taccgccgcg accatcaccc
     2761 agcaggtatc gctgctggaa tcgttcggca cggtcaaggt gctgtccagc cccaagctga
     2821 gcgtgatgaa caaccagacc gcgatactgc gcgtggtgga caatctggtg tacttcgtca
     2881 tcaatgtcac caccaccgcg ccgaccgcga ccagctcggc attcagcacg tttgcgacga
     2941 cgcccaacac cgtacccatc ggcttcacga tgagcgtgac tccgcaaatc aacgacagcg
     3001 acaccgtgca aatcaacttg cacccgtcca tttcgcggct gatcggttac gtcaacgacc
     3061 ccaacccgtc gctggccacc gcaggcgtcg tcagccgcat cccggtcacc tcgacgcgcg
     3121 aaatggaatc gatcctgaaa atcgagaaca accagatcgc cgtgatgggc gggctgatgc
     3181 aggactcgat caacaacctc accgacgccg tgcccggcct gtccggcatc ccgctggcgg
     3241 gcgagctgtt caaacagcgc aacgacacat ccaccaagac cgaactggtg atattcctgc
     3301 gcccggtggt catcaaggat gccagcatcg ccggagactt cagcgcatac cgcaacacgc
     3361 tgccggaccg gaatttcttc caggacccgg cgcacgccca accttaggaa gcgccgacca
     3421 tgagcctgct gctggatgcc cgcaagaaat ctcagctagc cctctcggcg caaggcgatg
     3481 agcgcatcca gacagcaccg gacaacaata accgcagcgc cggacaaaac ctgttcaacg
     3541 cgaaatcgcc gctgaaccgg attgccggtt tcggcatgca gcgcaatttg ctgctggcgc
     3601 tgggcggcac ggcactgttg ctggtcggcg gagccgtgta cctgtggcac ctcggctcat
     3661 ccagcagcac ccgcccgctg caaccgaaca ccgccagcca gccggtggcg gcaagcccgc
     3721 aacccgcgtc cgccgcaact ccgcccgccc ccgttgcggc gctggccgct cccgccgaca
     3781 ccaaaacgcg cgcccctatc tcacccgccg ccatcgcgcc aaccgaaagc gcaccgctgc
     3841 cgctcgcgcc agcgcgcaag gcgatacgca tcgaacggca atccgccgaa cgcatcgatc
     3901 ccgcgcccga caacgcctat cgggcatatc gcgacggcaa gctggacgag gcgcagcaac
     3961 tgtatcgcga gctgctcggc aaggacgcgc acaataccgg cgcgctgctc ggcctggcgg
     4021 ccatcgcgca acaactgggc gaggaaacat tggccgcaca atactatgcc gacgtgctgc
     4081 ggctcgatcc gcgcaacgcg acggccaatg ctggcatggc ggcgctgaac gccgacgaga
     4141 acagcgagag ccgcctgaaa accctgctcg gggagcagcc ggattccgcc gcgctgcatt
     4201 tcgcgctggg caacctgtat gccgggcaag cccgctgggg agaggcgcaa caggcctatt
     4261 tcaacgccag cgcgctggaa cccgacaacg cggaattcgc cttcaatctc gcggtcagcc
     4321 tcgaccatct cggtcagagc aagctggccg cacagcaata ccggcgcgcg ttgcagctcg
     4381 acggacaaaa ccgcgcgggc ttcgaccacg cccaaaccga acaacgcctg caagcgctga
     4441 cgcgctgaac cgccatgccg gacagcctcc aacaaccgct gggacgcata ctggtcgata
     4501 aaggcgtcat cagcgacgac cagttgcgca tcgcgctgca agaacagaac aaaacgcatc
     4561 agccgctggg ccgcctgctg gtccggctgg gctttttgtc cgaagccacg atacgcgacg
     4621 tgctgtcgga aaacctcggc caggaaagcg tggatctgtc cgccgtcatc atcgaccccg
     4681 ccgcgctggc gctgatcccc aaggacgtcg cgcgccgcta ccagttgctg ccgctgtcgc
     4741 tcgacccgca cagc
//
LOCUS       DASUIV010000029         8893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1029275, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000029 DASUIV010000000
VERSION     DASUIV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029275"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..131
                     /gene="rrf"
                     /locus_tag="VFW59_00930"
     rRNA            18..131
                     /gene="rrf"
                     /locus_tag="VFW59_00930"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            298..1497
                     /locus_tag="VFW59_00935"
     CDS             298..1497
                     /locus_tag="VFW59_00935"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5362811.1"
                     /translation="MSRQIQEAYIVAATRTPVGKAPRGMFRNTRPDDLLAHVLKSLLL
                     QCPNMEPSAVEDVIVGCAMPEAEQGMNVARIALLLAGLPYGVPGVTVNRFCSSGLQAI
                     ALAADRIRLGEAGVMIAGGVESMSMIPMMGNKVAMNSDIFAREENVAIAYGMGITAER
                     VAERWKVTREAQDAFSHESHRRALAAAASGAFQNEISPYEISEHQPDLAIHALRVANR
                     VVMHDEGPRADTSLEALSKLRPAFAKEGTVTAGNSSQMSDGAAAMLLCSEAALKRYNL
                     TPIGKFLGYSVAGVAPEIMGIGPKVAIPLALRQTGLHLQDMDWIELNEAFAAQALAVI
                     DDVGLDREKVNPLGGAIALGHPLGATGAIRAATLLHGLRRNQQKYGMVTMCIGTGMGA
                     AGIFEAL"
     gene            1558..2181
                     /locus_tag="VFW59_00940"
     CDS             1558..2181
                     /locus_tag="VFW59_00940"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX5362812.1"
                     /translation="MNGGIGSNDDWFVERIKRKMLMKKLIAILLLCVSMQTMAQELGG
                     AKFDDGVRVDNAPLVLNGVGLRSILFLKMYVAGLYLPEKMTNAEAVLANKGAKRIALH
                     VLNDTDSEHFLKGFRKGFEKNHNAQEMAELRDRMEAFVHMFDAVTKVKKGDVIALDWR
                     PENGTRIRLNESELGTIAGEDFYRALLSIWIGEKPVSSDLKKGLLGG"
     gene            2174..3412
                     /locus_tag="VFW59_00945"
     CDS             2174..3412
                     /locus_tag="VFW59_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362813.1"
                     /translation="MADWATRLNADCHAGLLDGDNLRRALRAEGEDFLSAVQADRPHL
                     FSGVAYFVSSAQMEAMQAVVAAVESVVTLSGWHDATWQKSAPAVRTSPFNLGVFFGYD
                     FHLNADGAHLIEINTNAGGAFLNALFIDSQREAVLPGECAAVTDCELAFVEMFRNEWR
                     LARGEMPLHCIAIVDEQPHQQYLYPEFVLAQRMFERAGIRALIADPSELMVRADGLYC
                     AEQKVDLVYNRLTDFTLGQHAILADAYRDDLAVLTPHPHAYVRYADKRNLALLTDAVA
                     LRAMGVADSVIATLMAGIPQTRLVQAQDHDQWWSERKQWFFKPAAGFGSKGSYSGAKL
                     TKRVFEEIMQGGYVAQRIAVPGERVVCLDGAEPQSLKSDVRCYVYDGRVQLLAARLYQ
                     GQTTNFRTPGGGFSPVRVVE"
     gene            3422..3811
                     /gene="crcB"
                     /locus_tag="VFW59_00950"
     CDS             3422..3811
                     /gene="crcB"
                     /locus_tag="VFW59_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX5362814.1"
                     /translation="MLIYSFLAIGIGAALGAWLRWGLGLWLNHAWPTLPLGTLSANLL
                     GGYLIGLAIAFSLQHPGLSPEWRLFVITGFLGGLTTFSTFSAETVTLLLRGQYLWGAG
                     IIAAHLGGSLLMTVMGIQTIKWLQGGQ"
     gene            3811..4116
                     /locus_tag="VFW59_00955"
     CDS             3811..4116
                     /locus_tag="VFW59_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX5362815.1"
                     /translation="MNSLIFYAEEKQTHAGMLFYEWLLKEARELGVHGGSVFRAIAGF
                     GRHDRLNEEPFFEPAGELAVKVEFILDDKLADQLLQRVKAQGMAVFYTRYVVQSGII"
     gene            4128..4919
                     /locus_tag="VFW59_00960"
     CDS             4128..4919
                     /locus_tag="VFW59_00960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5362816.1"
                     /translation="MKKIAIALLAILSVSAAQAAVQGKEVSYRSNGTVLKGYIAYDDA
                     VHGRRPAVLVVHEWWGHNDYARKRADMLAELGYTALAVDMYGDGKQAQHPDEAGKFAA
                     EVSKNMPVAEARFRAGMKLLRKQKTVDAKQFAAIGYCFGGGVVLHMARIGESLKGVAS
                     FHGSLGTEHPAQHGMVKARVLSFTGEDDPMIGADKVAVFKQEMEQAGANYRVVTYPGV
                     KHSFTNPAADELGKKFNLPLAYNAEADKDSWQQTTVFLREIFGGK"
     gene            5343..6485
                     /locus_tag="VFW59_00965"
     CDS             5343..6485
                     /locus_tag="VFW59_00965"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX5362817.1"
                     /translation="MLLSIYRYMPADGWDKPLDAELDSEHTLLICFGASNTDDLASGI
                     ADLGRVFPRSVWIGCSTAGEIFERSLEDGSLIVAVVKFNRTALHLASCEITDPNTSYQ
                     TGAYLAGSLSTPGLRGMFVLSDGLSVNGSELARGLSEHLPQNVVVTGGLAGDGDRFQK
                     TWVLVDRAPRSQHVVAVGLYGEYVGIGHGSRGGWDVLGPEREVTHAEGNVLYTLDGQP
                     ALALYKKYLGERAAGLPATGLLFPLAIRNELEEDGLTVRTILAVNEAENSITFAGDIP
                     QGGFVQLMRANFERLIDGAADAAKQIALGNYAGGPLLTVAISCVGRRLVLAQRIEEEI
                     EAVFDSLPVDGRLIGYYSYGELSPLSSGRCDLHNQTMTLTSFWENE"
     gene            6490..8544
                     /locus_tag="VFW59_00970"
     CDS             6490..8544
                     /locus_tag="VFW59_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5362818.1"
                     /translation="MHRLLARQLRKFGLDEQSPPDAASWSKFLAVIEQNYHDVDQDRY
                     TLERALAISSEEMHQLYRRQKSSYETRLRILFGTIQDMIWLKDPDGVYLACNPMFERF
                     FGASEAEIVGKTDYDFLNKELADFFREHDRLAMSKGSPSVNEEWVTFADDGHRALLES
                     VKTPMMDESGKLIGVLGIARDITRRKQEEEELTRYKDHLEEEVQQRTVDLVLARNAAE
                     SANRAKSVFLASMSHELRTPLNAILGFSSVMRKDPQLSQAQRENLDIINRSGEHLLTL
                     INDVLEVAKIEAGRVQVERAPFDLGHMVRDVTDMMLIRAQEKGLQLLLDQSSEFPRYI
                     KGDEARLRQVLINLVGNAVKFTRQGGVTVRFGLKPHIAQQRLVIEVEDSGPGISPEDQ
                     QRIFEPFVQVGEPSDQKGTGLGLTITRQFVQLMGGTISLESMPGKGSIFRIEFPVDKV
                     AAAEVPRAESTGKNEIIGLSPGQPEYRILIVEDQLENQLLLSKLMENVGLQIRVAENG
                     VRALELFQSWHPHLIWMDRRMPVMDGLETARHIRELPGGKEVKIIAVTASAFMEQRDE
                     MLNAGMDDFVRKPYRFNEIYECLGRQLGVQYIYADKSSTEEEKEEAMTAPMLAVLPPN
                     LRHELHDALESLESERITGVIQQVAPLDAKLHKSLCKLAANYNYPAILNALQALPPVK
                     TP"
     gene            8541..>8893
                     /locus_tag="VFW59_00975"
     CDS             8541..>8893
                     /locus_tag="VFW59_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5362819.1"
                     /translation="MNADKGKILAVDDTPESLRLLTDILTAEGYEVRSAISGELALRA
                     ATIHPPELVLLDIRMPVMDGYEVCRHLKAQAETHNVPVIFVSAAMETEDKVRGFELGA
                     VDFVTKPYQRDELLA"
BASE COUNT         1869 a         2132 c         2844 g         2048 t
ORIGIN      
        1 gaatgtcgaa cagtttgtct gacgaccata gcgagttggt cccactcctt cccatcccga
       61 acaggacagt gaaacgactt agcgccgatg atagtgtgga ttacccatgt gaaagtaggt
      121 catcgtcaga ctccttacaa agcaaacccc ctccagaaat ggtgggggtt tttgctttta
      181 taacgcctaa ctccgccctg aacgtggcta cctgtgcctc attcgggtat tctgcgtgcc
      241 agaaggtctc gggcagagcg cggggcgtat tcactaggtc agattgcaag gggcatcatg
      301 agtcgacaaa tccaggaagc ctacatcgtt gcagcgacgc gcacgcccgt gggtaaggcg
      361 ccgcgaggca tgtttcgcaa cacgcgtccg gatgatttgc tggcacatgt gttgaagagc
      421 ctgctgctgc aatgcccgaa tatggagccg agtgcggtgg aggatgtgat cgtcggctgt
      481 gccatgccgg aggcggaaca gggtatgaac gtcgcgcgca tagcgctgct tctggcgggc
      541 ctgccttatg gtgtgccggg ggtgaccgtg aaccgttttt gttcgtcggg gctgcaggca
      601 atagcgctgg ctgccgaccg cattcgtctc ggagaagcgg gcgtgatgat cgcgggcggg
      661 gtcgagagta tgagtatgat tccgatgatg ggtaacaagg tggccatgaa ctcggatatc
      721 tttgcgcgtg aggaaaatgt tgccattgcc tacgggatgg ggatcactgc ggagagggtg
      781 gcggaacgtt ggaaggttac acgcgaggct caggatgctt tttcccatga gagtcatcgt
      841 cgtgcattgg cggcagcagc ctccggcgct tttcaaaatg agattagtcc gtatgaaatc
      901 agtgagcatc agcctgactt ggctatacat gcactgcgcg tcgccaatcg cgtggtgatg
      961 cacgatgaag ggcctcgtgc cgatacttca ttggaggcat tgtcgaagtt gcgtccagcc
     1021 ttcgctaagg aaggaactgt gacggcaggc aacagctcgc agatgtcaga tggtgctgcg
     1081 gccatgctgc tatgcagcga ggctgcgctg aagcgctaca atctcacgcc tattggaaaa
     1141 tttctcggct actcagtcgc gggggtggcg cctgagataa tggggatcgg tccgaaagtg
     1201 gcgatcccgc ttgctttgcg ccagacgggt ttgcacttgc aggacatgga ttggatcgaa
     1261 ctcaatgagg cattcgccgc tcaagctctg gcggtgatag acgatgtcgg gctggaccgg
     1321 gagaaagtga atccgttagg tggcgcgatt gcgttaggtc atccacttgg cgcgacgggg
     1381 gcgattcgcg ctgccaccct gctgcatggt ctgcggcgga accaacagaa gtacggcatg
     1441 gttacgatgt gcattggcac aggtatgggg gccgccggaa tctttgaggc gctctagaga
     1501 agggttcgtt cgtgttgtcg gctgtgcttg aaggttggcg cgactgaacg gttgctgatg
     1561 aatgggggga tagggtctaa cgacgattgg tttgttgaac gaataaaacg aaagatgcta
     1621 atgaagaaat tgattgcgat tttgttgttg tgcgtatcga tgcagacgat ggcgcaggaa
     1681 ctgggggggg cgaagttcga cgatggggtg cgggtggata atgcgcctct ggtgctgaac
     1741 ggtgttggat tgcgcagtat tctgttcctt aagatgtatg tcgcaggttt atatttgccc
     1801 gagaagatga ccaatgcaga ggctgtactg gcgaacaagg gcgccaaacg tatcgcactg
     1861 catgtgttaa acgataccga cagcgaacac ttccttaagg gttttcgcaa agggttcgag
     1921 aaaaaccata atgcacagga aatggcggag ctgcgtgatc ggatggaagc tttcgtgcat
     1981 atgttcgatg ctgtcacgaa ggtgaagaag ggcgatgtga tcgccctcga ttggcggccg
     2041 gaaaatggca cccgtatccg cctgaatgaa tcggagctgg gcacgatcgc aggcgaggat
     2101 ttctaccgcg cgctgctttc aatctggatc ggcgagaagc ctgtgagcag cgatctgaag
     2161 aagggtttgc tgggtggctg attgggcgac gaggctgaat gccgattgcc atgctggatt
     2221 gctcgatggc gataatctga ggcgcgcgct acgcgcggag ggtgaagact tcctcagtgc
     2281 agtgcaggca gatcgccctc atcttttttc gggtgtggct tatttcgtct cttccgctca
     2341 gatggaagcg atgcaagcgg tggttgctgc agtcgagagc gtcgtgacgt tgtcaggctg
     2401 gcatgatgcg acgtggcaga agtccgcacc tgctgtgcgg acatcgcctt tcaatctcgg
     2461 tgtgttcttc ggctacgatt ttcacctcaa tgcagacggt gcccatttga tcgagatcaa
     2521 taccaatgca ggaggggcat ttcttaatgc gctgtttatc gacagccagc gtgaagcagt
     2581 gctgcctggc gagtgcgcgg cagtgactga ctgtgagctg gcgttcgtcg agatgtttcg
     2641 taacgagtgg cggctggctc gcggcgagat gccattgcat tgcattgcca tcgtcgatga
     2701 acagccgcat cagcagtacc tctatcccga gttcgtgctg gcacagcgca tgttcgaacg
     2761 tgcgggtatc cgtgcgctga tcgctgatcc atccgagttg atggtgcgtg cagatggctt
     2821 gtactgcgcg gagcagaaag ttgatctggt ttataaccgg ctcacggatt ttacgctggg
     2881 gcagcatgcc atactggccg atgcataccg cgatgatctg gcggtgttga cgccgcatcc
     2941 ccatgcttat gtgcgctatg cggataagcg caacctcgcg ttgcttaccg atgccgtggc
     3001 gttgcgtgcg atgggggtgg ccgattcggt catcgccaca ctgatggctg gcattcctca
     3061 gactcgtctg gtgcaggcgc aagatcacga tcagtggtgg tccgagcgca aacagtggtt
     3121 ttttaaacca gccgcaggct tcggcagcaa gggcagttac agcggtgcga agctgaccaa
     3181 gcgtgtgttc gaggagatta tgcagggtgg ctacgtcgcg caacgcatcg cggtaccggg
     3241 cgaacgcgtg gtgtgtctgg atggtgccga gccacaatcg ctcaagagtg atgtgcgctg
     3301 ttatgtctat gatgggcgcg tgcaattgct tgccgcgcgg ctgtatcagg ggcagaccac
     3361 caactttcgc acacccggag gcggcttctc acccgtgcgc gtggtcgagt agaatgaccg
     3421 catgttgatc tattcgtttc ttgcaatcgg aatcggcgcc gctctggggg cgtggttacg
     3481 ctggggcttg gggctgtggc tcaatcacgc ctggcccacg ttgccgctcg gtacgctgtc
     3541 ggccaatttg ctcggcggtt atttgatcgg actggcgatt gctttttctc ttcagcatcc
     3601 gggactctcg ccggaatgga ggctgttcgt catcaccggc ttcctcggtg gattgacgac
     3661 cttctcgacg ttctccgcag agacggtcac gctgttgttg cgtgggcagt atctgtgggg
     3721 tgcgggcatc attgcggccc atcttggcgg ctcgttgctg atgacggtca tgggaattca
     3781 aaccattaag tggttacagg gagggcagtg atgaatagct taattttcta tgcagaagag
     3841 aagcagaccc atgctggcat gttgttctac gaatggttgt tgaaagaggc tcgcgaactc
     3901 ggtgtgcatg gtggctcggt gtttcgagcc atcgccggat tcgggcgaca tgaccgattg
     3961 aacgaagagc catttttcga acccgcagga gaactggccg tgaaggtcga attcatcctc
     4021 gatgacaaac tcgcggatca gttgttgcaa cgcgtgaagg cgcagggtat ggcagtgttc
     4081 tacacgagat atgtggtgca gtccggaatc atttgagtgg aggaaatatg aaaaagatcg
     4141 cgattgcgtt actcgctatt ctgtctgttt cagcggcgca ggctgccgtg cagggcaagg
     4201 aagtaagcta ccgttcaaac ggcacggtac tcaagggcta tatcgcctat gacgatgcgg
     4261 ttcatggtcg ccgtccggca gttttggtgg tgcatgagtg gtgggggcac aacgactatg
     4321 cgcgcaaacg tgccgacatg ttggctgaac tgggctacac cgcgctggcc gtggatatgt
     4381 atggtgacgg caagcaggct caacatccgg atgaggcggg caagttcgca gccgaagtga
     4441 gcaagaatat gccggtggcc gaggcgcgat tcagggccgg catgaagctg ctgcggaaac
     4501 agaagacagt ggatgcgaaa cagttcgccg caatcggcta ttgctttggc ggcggggtgg
     4561 tgctgcacat ggcgcgtatc ggcgagagcc tgaagggcgt ggcgagtttt cacggctcgc
     4621 tgggaaccga gcatcccgcg cagcacggca tggtcaaggc gcgagtcctt tctttcaccg
     4681 gagaggacga tccgatgatc ggtgctgaca aagtggcggt cttcaaacag gaaatggagc
     4741 aggctggtgc gaactatcgt gtggtcactt atcccggtgt taaacatagc ttcaccaatc
     4801 ctgccgctga tgagttgggc aagaaattca atctgccatt ggcctataac gccgaggccg
     4861 acaaggattc ctggcagcag accactgtgt tcctgcgcga gatatttggc ggaaaataag
     4921 ttgcatttga tttgaatgat gacgccccgc tcaggggcgt ttttttgtct gaaatctctg
     4981 cgacggctga tatcggccac gcattttgaa aagtctgccg tatcgggagt gacgggtata
     5041 gcaagatgag ccccatccct tcgtcgtttg cctattcgca tcttcccgga atacgactag
     5101 aaaatagtct ttcccttata tttctttagg ctttgcttgg gcaaagttcg ttacgtagat
     5161 ctccgtcggt gtgaaacctg gttctgcgcg ttgcggcgaa gattacttct ctcccctgtc
     5221 gacaactcaa gggccgcatg tgctcagtca tgatttctgc tgggtcttgt gagcggtgtt
     5281 tgctggactt attgtcgatg cttgatatgt tgaacggaaa acttcgtgag agggaataag
     5341 ccatgttgct gtcaatctac cgatatatgc ctgctgacgg atgggataag ccgctggatg
     5401 cggaattgga ctcagaacac accttgttga tttgttttgg cgcatccaac accgacgatc
     5461 tggcatcagg cattgccgat ctgggcaggg tattcccccg ttcagtctgg atcggatgtt
     5521 cgactgctgg tgagattttc gagcgttcgc ttgaggatgg ttcgctgatt gtggccgttg
     5581 ttaaattcaa tcgtacggcg ttgcatctgg caagctgtga aattaccgac cccaatacct
     5641 cgtatcaaac cggggcttat ctggcaggat cgctgtcaac gcccggattg cgaggcatgt
     5701 tcgtattgtc cgatggtctg tcggtcaatg gttcggagct ggccagaggc ttgtcagagc
     5761 atctgccgca aaacgtggtg gtcacgggtg ggctggcggg tgatggggat cgctttcaga
     5821 aaacctgggt gctggtggac agggcacctc gttctcagca tgtcgtggcg gtgggtttgt
     5881 acggtgaata tgtgggcatt ggtcatggat cacgaggcgg atgggatgtg ttggggccgg
     5941 agcgcgaggt tactcacgcc gaggggaatg tgctctacac cctggatggt cagcccgccc
     6001 tggccctgta caagaaatat ctcggcgaac gcgcggcggg cctgccggcg accgggctgc
     6061 tgttcccgct tgcgattcgg aatgagctgg aagaggacgg tttgaccgtg cgcaccattc
     6121 tggccgtcaa cgaagcggag aactccatta ctttcgcggg tgatatcccg cagggcggat
     6181 tcgtacagtt gatgcgagct aactttgagc gcctgatcga tggggccgcc gatgccgcga
     6241 agcaaatcgc gctgggcaac tatgcgggcg ggccgctgct cacggtggcg attagctgtg
     6301 tcggacgccg gctggtactg gcccagcgca tcgaagaaga gattgaggcc gtgttcgata
     6361 gcttgccggt cgatggccga ctgattggtt attactccta cggtgaactg tcgcccctgt
     6421 ccagcgggcg ttgtgatctg cacaaccaga ccatgacgct gacctcattc tgggaaaatg
     6481 aataacgtca tgcatcggct attggctcga cagctgcgca aatttggact ggatgaacag
     6541 agtcctcccg atgcagctag ctggtcgaaa ttcttggccg tcatcgaaca gaactatcac
     6601 gatgtcgatc aggatcgcta tacgctggag cgggctctgg ccatttcatc tgaagagatg
     6661 catcagctgt atcggcgcca gaagtcttcc tatgaaacgc gactgcgtat cttgttcgga
     6721 accatacagg atatgatttg gttgaaagat cctgatggtg tttatcttgc ttgcaatccg
     6781 atgtttgaaa gatttttcgg tgcgagtgag gctgaaatcg tgggcaagac agactacgat
     6841 ttcctcaata aggagcttgc cgatttcttt cgagaacatg accggctcgc catgagcaag
     6901 gggagtccca gcgtcaacga ggaatgggtg acctttgccg atgacggcca tcgcgccttg
     6961 ctggaaagcg tcaagacgcc aatgatggat gaaagtggaa agctgatcgg ggtgctggga
     7021 attgcccggg atatcacaag gcgtaagcag gaagaagagg agctgacccg ctataaggat
     7081 catctggaag aagaggtcca gcaacgcacg gtagatctgg tgctggcgcg caatgcggcg
     7141 gagtcggcga atcgggccaa gagcgtgttt ctggcgagca tgagccatga gttacgaact
     7201 ccgctgaatg ccatactcgg cttctccagc gtgatgcgca aagacccgca gttgtcgcag
     7261 gcgcagcgcg agaatctcga tatcatcaac cgtagcggcg aacatctgct gaccctgatc
     7321 aacgatgtgc tggaagtagc caagatcgaa gcgggccgag ttcaggttga gcgtgctcct
     7381 ttcgaccttg gtcatatggt gcgcgatgtc accgatatga tgttgatccg cgcacaggag
     7441 aaagggctgc agctgttgct ggaccagtct tcggagtttc cgcgctatat caagggcgac
     7501 gaggcgcgtt tgcgtcaggt gctgatcaat ctggtcggca acgcggtgaa gttcacccgg
     7561 caggggggcg ttaccgtacg tttcgggctt aagcctcata tcgcgcagca acgcttggtg
     7621 atcgaggttg aggactccgg ccccggcatc tctccggaag accagcaaag aatcttcgag
     7681 ccattcgtgc aggtgggaga gccgagtgat cagaagggca cggggctggg gttgacgatc
     7741 acccggcagt ttgtgcagct gatggggggc acgatcagtc tcgagagcat gccgggcaag
     7801 ggttcgatct ttcgcatcga gtttccggtg gacaaggtcg ctgctgcgga agtccctcgg
     7861 gcggaaagca caggaaaaaa cgaaattatc ggcttgtccc ccggtcagcc tgagtaccgc
     7921 atcctgatcg ttgaggatca gttggagaat caattgctgc tcagcaagtt gatggaaaat
     7981 gtcggcctgc agatcagggt ggcggagaat ggcgtgcggg ctctggagct ctttcagagc
     8041 tggcatccgc acttgatctg gatggacagg cgcatgccgg tgatggacgg tctggaaacg
     8101 gccaggcata tccgcgaact gccgggtggc aaggaggtga aaatcatcgc ggtcacggcc
     8161 tcggctttta tggagcagcg cgacgaaatg ctgaatgcgg gcatggatga ttttgtgcgc
     8221 aagccttacc gtttcaatga aatctatgaa tgtctgggca ggcagctggg cgtgcaatat
     8281 atttacgccg ataagagcag cacagaagag gaaaaagagg aggcaatgac ggcgccgatg
     8341 ctggcggtgt tgccaccgaa tttgcgccac gaacttcacg atgcgctgga aagtctggag
     8401 agcgaacgga ttactggcgt gattcaacaa gtcgcccccc tggatgcgaa gttgcacaag
     8461 tcgctctgca agttggcggc aaattataac tatcccgcga tcctgaatgc tttgcaggcc
     8521 ctgccaccgg taaagacgcc atgaatgctg acaagggcaa gatactcgcg gtcgacgata
     8581 cgccggaatc attgaggtta ttgacggaca ttctgacggc agagggctat gaggtgcgtt
     8641 ccgcgattag cggtgagctg gccttgcgcg cggccacgat ccatccgcca gaattggtgt
     8701 tgctcgatat ccgtatgccg gtgatggacg gatacgaagt ttgccgacac ctcaaggctc
     8761 aggcggaaac gcacaatgtg ccggtcattt tcgtcagcgc ggcgatggaa acagaagaca
     8821 aggtgcgagg gttcgaactc ggtgctgtcg atttcgttac caagccctat cagcgtgacg
     8881 agctattggc cag
//
LOCUS       DASUIV010000030         9116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1029501, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000030 DASUIV010000000
VERSION     DASUIV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029501"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFW59_00980"
     CDS             complement(<1..236)
                     /locus_tag="VFW59_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX5362820.1"
                     /translation="MTERAGIEIDYCPQCRGVWLDRGELDKIIERSMGELAQIAPVMP
                     SSRPVQQPYDNNHGYHGKHHDGRHGYRGKKSLWQ"
     gene            complement(669..2111)
                     /locus_tag="VFW59_00985"
     CDS             complement(669..2111)
                     /locus_tag="VFW59_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX5362821.1"
                     /translation="MVSSIGELLVRESIISMAQLNEALSYQKQHGGRLGDSLSALKYI
                     SPSELKQFFNSIPPVPLKIDQTGLSETLLTDLLLKIAYFDGGTYSLGSMSKSMSLPMG
                     VVDELTELARNDSLIAIKSAAGYNRASHVFELTGQGRKRAGEALEQSQYAGPAPVPLK
                     FYSIMTAHQTVRQIEIDEPWVRHALSHMVVDEDLLDRLGPAFSSGRSIFMYGPPGTGK
                     SSITESLARALTTSIYIPYAIEVGGQIIRLFDPAMHIPVEEAGEEGPQLDLESTHKYD
                     PRWRLCRRPVVMVGGELTLQSLDLDFDPTTKFYEAPIHMKAANGFFILDDFGRQMVPP
                     RQLLNRWIVPLERGTDFLSLHTGKKFEIPFDQVTVFCTNLKPSELVDEAFLRRIRHKV
                     RIGYQTEEQFYEILRRICEQQGIVYTDAAARYLVVTYYRQVNRPLVGSHPRDLVEQII
                     DRARFMKKHPELTPETIDAAAENYFVDMDA"
     gene            complement(2276..3559)
                     /locus_tag="VFW59_00990"
     CDS             complement(2276..3559)
                     /locus_tag="VFW59_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5362822.1"
                     /translation="MKNRVKFLSAVSIIPLLSACASDSNTANREAWAIRPVYNVRHST
                     ETPTSYYQLGRYYQGQNRYQQALSAYQKALEIDGSFVEARNGMGVVFARQGRLPEAIE
                     QFSLAERLAPNAAHIRNNLGYALYLSRSYAAAASELERAVALEPSNQSARFNLALALD
                     KAGDTEQAVQVMAQAAKAQFSEKVPTVDAQRVPADALSLAPASAVTPPPTPAPMQVSA
                     LPKDRGVIAQAAPKPVALVESRSQSIPLAPSVYEQRVRADARVVPAAGSLQQISAQPK
                     EPDRVVVQAAYKPVPAVSVSPVLSARGLTEPQTLRAAAPTAKPYRIEVSNGNGVAGLA
                     RKVAGFLDGEGYSGARLTNQKTFKVASSQVQYRSGFREQAQSLVLTLPGQPGIAQVED
                     LRRDISIRVVLGKDVASNVASFEHGQDKVRLARQE"
     gene            complement(3581..4528)
                     /locus_tag="VFW59_00995"
     CDS             complement(3581..4528)
                     /locus_tag="VFW59_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5362823.1"
                     /translation="MTTAYYLIIVFVVVFGVALVILRQLAVRPLQKRLDGVAADSSDV
                     PLQQAAPTWIKRVVELSGPLARLTLPKEGWESSNLRIRFMNAGYRFPSAPVVFFAAKT
                     LLALIFPVMLALYFGLGGAEKIKPAMLLSLLIGVAALGYLLPNAWLMWRIRSRQLEIF
                     ESFPDALDLMTVCVEAGLALDAALVRVGEEMRFKSVILAEELHLVSLEMRAGSGRERA
                     LRNLALRTGVAEIDSLVALLVQSDRFGTSVADSLRIHSATLRTKRRLRAEEAAAKIAV
                     KLLFPLIFCIFPALMLVLLGPSYLTMFRVLFPALEGIQY"
     gene            complement(4557..5531)
                     /locus_tag="VFW59_01000"
     CDS             complement(4557..5531)
                     /locus_tag="VFW59_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5362824.1"
                     /translation="MDYLYYLFVILAFIAVVLFLEGSYLAWNAYKGPEAKRIEKRLRS
                     MSAEAQTNGDTSIIKKRLLAETPALDRLLLEIPRIHHLDRLLVQSGLTLNVASFLWLT
                     VMGASAGLIMGVFFGFPLFMVLAGGVALGALPLLYLLRAKQKRMASFEEQLPDALDLM
                     GRALRAGHAFSGALKLVGDEMPDPVATEFRTTFDELNYGVSLQNALQNLAVRAPSDDL
                     RYFVIAVLIQRESGGNLAELLDSISALIRERLKLIGTIRVFSAEGRLSAWILGILPFV
                     MAFVMSVLNPKLMSMLWTDPEGLKLIRIVFVMMIIGVFWMRRIIRIHV"
     gene            complement(5541..6899)
                     /locus_tag="VFW59_01005"
     CDS             complement(5541..6899)
                     /locus_tag="VFW59_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX5362825.1"
                     /translation="MSLRDRLENIKHDGTQMPVVTPQVTAAENRSYQELKSRIHQKLL
                     DRVDLSVMESLSQDRLQEELKVLVERLLTEETLAINDTERKNLVRDIQHEVLGLGPIE
                     TLLADPTVSDILANTYRQVFVERAGKLELTDVRFEDDTHLMKIIDKIVSGVGRRVDES
                     SPMVDARLRDGSRVNAIIPPLAIDGPILSIRRFSVTPLGMDDIIRGKSLTPEMAEIIG
                     GLVKAKVNLLISGGTGTGKTTLLNIMSGFIPRAERIITIEDAAELQLQQPHVVRLETR
                     PPNIEGKGEVAQRALVKNSLRMRPDRIILGEIRGAEALDMLQAMNTGHEGSMTTVHAN
                     SPRDSLTRLENMVGMAGLNLPTKAMRQQISSAITAIIHVSRLSDGKRKITSIQEITGM
                     EGDIVTMQDVFVFEQTGVASDGTVRGHFHATGIRPRFIDLLHTRGVNISDDIFDPTRL
                     YE"
     gene            complement(7025..8191)
                     /locus_tag="VFW59_01010"
     CDS             complement(7025..8191)
                     /locus_tag="VFW59_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5362826.1"
                     /translation="MKIAVLSPNKSQREGVVHILQEGEQLHVVLSFDGSEEQLEAVAD
                     QQLPDLIVIESAGDGQGAIAALGRVSQRHAGMSFIVLCEQVSAENLIQLMHLGVRDVL
                     PVPVARQALVEAVARVEQKHARTEMPLHKGKVLAFIACKGGSGATFLASNLGYVLATE
                     YGKRVALIDLNLQFGDASLFVSDKVPTITLSDVVGNISRLDASLFASSMVKVLPNFGV
                     LAAPENPAKAADVWPSHIEVLMRLAVAEYDYVILDIGRNLEAVSIKALDHADLIFPVL
                     QETLPFVRDAKRIVSTLQSLDYSKDKIHLIVNRYEKGGDIQLADVERTLGMKVMLTFP
                     NSYVAVSASVNQGVPILKMARADPVAKILLKLGHDLELGEEEKTGWFGRLFSRA"
     gene            8274..8654
                     /locus_tag="VFW59_01015"
     CDS             8274..8654
                     /locus_tag="VFW59_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362827.1"
                     /translation="MRNKPKLSVGITTVEGGKGIGGKGNQSSVPISGVKVALTLTLTK
                     PIQFCGVPTPSGFMQLLAFSSTLSGKSLPVKTTISALRKGSMDIFAVESSGAAGSDDS
                     PINPLEFIIVFQSLRQQVTDSLAT"
     gene            complement(8702..8809)
                     /locus_tag="VFW59_01020"
                     /pseudo
     CDS             complement(8702..8809)
                     /locus_tag="VFW59_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
     gene            complement(8812..>9116)
                     /locus_tag="VFW59_01025"
     CDS             complement(8812..>9116)
                     /locus_tag="VFW59_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362828.1"
                     /translation="ETVDVCNSRLGSVVTNARSMVATAATDSGVTDFDATKVEVRCDG
                     SICSSNQAVLSSIHVTVAIVDYGIRLGQWLPLSYSGGSAGYNLGIQPYTTMRYMGG"
BASE COUNT         2150 a         2738 c         2325 g         1903 t
ORIGIN      
        1 tcttgccaga gtgatttttt gccacgatag ccatggcgtc catcgtgatg tttgccatgg
       61 tagccgtgat tgttgtcata tggctgctgt accggacggg acgacggcat caccggtgcg
      121 atctgcgcca gctcgcccat gctacgttcg atgattttat ccagttcgcc acgatcgagc
      181 cagactcccc ggcactgtgg acaataatcg atttcgatcc ccgcgcgttc agtcataagc
      241 agatcgatgc tggggcaggc gggacatttc atgttaatcc tttttaaata gatggccaca
      301 ccggaatcgc cgctggtctg gacttggttt gaaaaatatt tgtctggaac gcatcatcca
      361 tcgggctaca gaaggctgcg atgtccgctg ttttttgcag ctttccggtc agtcgttcgg
      421 cgcagcctgt gccgcagtat caatactgcg ggagcgtgaa tgaagccgac tatttccgcc
      481 atgcccagat tctttgagcc acaggcatgc acggaattgc cgatgggaag actaacgggg
      541 caagcttaag cgtcgcttaa gtcaccttta atcacctatt gatatgcgcg gcacgatgcc
      601 gtttagttta agcgatggca tcgtcgccgc tgggcagagc cgagtggatc cgtgggagat
      661 gaaaacactc atgcatccat atcgacgaaa taattttcgg cggcggcatc aatggtttca
      721 ggcgtcaact cgggatgctt cttcataaac cgggcacggt ctatgatctg ttccaccaga
      781 tcgcggggat ggctccccac cagcggtctg ttgacttgcc ggtaataagt caccaccaga
      841 tagcgagcgg ccgcatcggt atagacgata ccctgctgct cgcaaatgcg tctgaggatc
      901 tcatagaact gctcttcggt ctggtagccg atgcgcacct tgtgacggat gcgacgcaga
      961 aatgcctcat ccaccaattc gctcggtttc agattggtgc aaaaaacagt gacttgatcg
     1021 aaggggattt cgaatttctt tccggtgtgc aatgaaagga agtccgtacc ccgctccagc
     1081 gggacgatcc aacggttcag caactgacgg ggcggtacca tttgccgacc aaagtcatcc
     1141 aggataaaga aaccattcgc ggctttcatg tggatggggg cttcataaaa tttggtcgta
     1201 ggatcaaaat ccagatccag cgattgcagc gtcaactccc cccccaccat gaccaccggg
     1261 cgacgacaca gacgccagcg tggatcgtat ttatgagtgg attccaaatc cagctgcggg
     1321 ccttcttcac ccgcttcttc aacggggatg tgcattgcag gatcgaacag ccggatgatt
     1381 tgcccgccaa cctcgatcgc atagggaatg tagatactgg tcgtcaatgc ccgcgccaga
     1441 ctctccgtga tgctggattt cccggtgccc ggtgggccat acataaaaat ggaacgcccc
     1501 gaactaaaag cggggccgag ccggtcaagg agatcttcat ccaccaccat atggctaagt
     1561 gcatggcgca cccaaggctc atcgatctcg atctggcgca cggtctggtg tgccgtcatg
     1621 atgctgtaga acttgagtgg cacaggggct ggcccggcat attgagactg ctccagagcc
     1681 tctcccgcac gtttccgccc ctgcccggtg agctcgaaga catgcgacgc acggttgtac
     1741 cctgcagcac tcttgatcgc gatcagacta tcgttccgcg ccaattccgt tagctcatcc
     1801 acaacgccca tcggcaggct catgctttta ctcatggagc ccagggaata agtaccgcca
     1861 tcgaaatagg cgatcttcag caacagatcc gtcaatagcg tttcactcaa tccggtctga
     1921 tcgatcttga gcggaaccgg aggaatcgaa ttaaagaact gcttcagttc actgggcgaa
     1981 atatacttca gcgccgacag tgaatcacct aagcgtcctc cgtgctgctt ctggtaggaa
     2041 agtgcttcat tgagttgcgc catggaaatg atgctttcgc gaaccagcag ttcaccaatg
     2101 ctagaaacca tacgccctcc ttcgataaaa gcgatttgac ctcaaactcg tgtggtcaaa
     2161 gacatctaat atgaaccgca acgccagctc ttgcagagtg gcaagaccga cgatgaacgc
     2221 gccctgtgaa acccgcccag accaaagacg aattcatcgg acaggctcag caagcttatt
     2281 cctggcgcgc cagacgaact ttgtcctgac catgctcaaa agaggcaacg ttgctagcga
     2341 catctttgcc caaaacgacc ctgatactga tgtccctgcg caaatcttca acctgcgcga
     2401 tgccgggctg tccaggcaag gtcagaacca gcgactgcgc ctgctcccga aagccggagc
     2461 gatactgcac ctgcgatgag gcgaccttaa aagttttttg attggtcaaa cgggcgccgg
     2521 aatacccttc cccatcgaga aagcctgcca cctttcttgc caaaccagcc actccgttgc
     2581 cattggagac ttcgattctg tacggtttag ctgtgggtgc ggcggctcgc aatgtctgcg
     2641 gttcagtgag gccgcgtgcc gacaagacag gcgagacact gacagctgga accggtttat
     2701 atgcagcctg aacgacaacc ctgtccggct ctttcggctg cgcagaaatt tgttggagcg
     2761 atccggctgc gggcactacg cgcgcgtccg cccgtacacg ctgttcatac acactgggcg
     2821 caagcggtat ggactgggag cggctttcca ccagagcaac cggcttcggt gcggcctgcg
     2881 caatcacgcc cctatccttc ggcaaggccg acacctgcat tggtgccgga gtgggaggag
     2941 gcgtaacggc tgaagcagga gcgagagaga gcgcatcagc cggcacccgc tgcgcatcca
     3001 ccgtcggcac cttctctgaa aactgcgcct tagcagcctg agccatgacc tgcacggcct
     3061 gctctgtgtc acccgccttg tccagcgcca gcgccagatt gaagcgcgcc gactggttgg
     3121 atggctccag cgcaaccgcc cgttccagtt cacttgccgc cgcagcataa gacctgctca
     3181 ggtacagggc ataacccaga ttgttgcgga tatgggcggc attcggtgcc agtcgctcgg
     3241 ccagactaaa ctgctcgatc gcctcaggca gtctaccctg cctggcgaag accacgccca
     3301 tcccgttgcg cgcctcgaca aacgagccgt cgatctccaa cgccttctgg taggcagaca
     3361 gcgcctgctg ataacgattc tgtccttggt agtaacgtcc cagttgataa taactggtcg
     3421 gcgtttctgt cgagtgtctg acgttataga ccggtctgat ggcccacgcc tcgcggttgg
     3481 cggtattgga atcagaagcg caagcgctca acaacggaat gatcgaaaca gccgagagaa
     3541 acttgactct gtttttcatg acacagctcc tttgagaatc ctaatactgt atcccctcta
     3601 gcgcggggaa caagacacgg aacatggtta gatacgatgg cccgagaagc accaacatca
     3661 atgcaggaaa aatacagaat ataagcggga acaataactt aaccgcaatc tttgcggccg
     3721 cttcttcggc ccgaagcctg cgcttggtgc gcagcgttgc ggaatgaatc cgcagcgaat
     3781 cagccacgct agtgccaaaa cgatcagact gaaccagcaa ggcaaccagc gaatcgattt
     3841 ctgcgactcc ggtgcgcaga gccagattgc gcagggctct ttcccgtcca ctgccagcgc
     3901 gcatctccag actgacgaga tgcaactctt ccgcgagaat cacgctcttg aaccgcattt
     3961 cctcgcccac ccgtacgagt gcagcatcca gcgccaaccc ggcttccacg cacaccgtca
     4021 tgagatccag cgcgtcgggg aagctctcga aaatttccag ctggcgactc ctgatacgcc
     4081 acatcagcca agcattaggc aagagatacc ctagtgccgc gaccccgatg agcaaggaca
     4141 acagcatcgc gggcttgatt ttttcggcac cacccaatcc aaaataaagc gccaacatca
     4201 ctggaaagat caaggccaac agtgtcttgg ccgcaaaaaa cacgacggga gcagaaggaa
     4261 aacgataacc cgcattcatg aaacgaatgc gcaggttgga actctcccac ccctccttcg
     4321 gcaaggtgag tctggcgagc ggcccgctca actcgacgac tcgtttgatc caggttggcg
     4381 cagcttgctg caacggcacg tcggaactat ccgccgcaac gccatccagc cgtttctgca
     4441 atggacgcac cgccaactgt cgcaggataa ccagcgcgac cccgaacacc accacgaaaa
     4501 cgatgatcag ataatatgcg gtagtcattt attgtccctc cccaaatgat ccaggttcat
     4561 acatggattc taataatgcg tcgcatccag aaaacgccaa taatcatcat cacaaacacg
     4621 attctgatca atttaagacc ttctggatct gtccataaca tgctcatcag cttgggatta
     4681 agcacgctca tcacaaaggc catcacgaac ggcaagatac ccagaatcca ggcagacaaa
     4741 cgcccctcgg cagagaacac ccgaatcgtc ccgatcagct tcagccgctc gcggatcagg
     4801 gcactgatac tgtccagcag ctccgccaga ttacccccgc tttcacgctg gatcagcacc
     4861 gcaatgacaa agtagcgcag gtcatcgcta ggcgcacgaa cggccaaatt ctgcaaggca
     4921 ttctggaggc tgacgccata attgagctcg tcgaaagtcg tgcgaaattc agttgccacc
     4981 ggatccggca tctcgtcccc caccaacttc aatgcacccg aaaaggcatg ccccgcccgc
     5041 aaagcccgcc ccatcaagtc gagcgcgtcg ggaagttgct cctcaaagga agccatgcgt
     5101 ttctgctttg cacgcagcag atacaacagg ggcagcgccc ccaatgccac gcctccggca
     5161 agaaccatga acagcggaaa tccaaaaaac acgcccataa tcaaacccgc cgatgccccc
     5221 atcacggtga gccagagaaa actggccaca ttgagcgtca atccggattg caccagcagc
     5281 ctgtccaggt ggtggatacg cggaatctcc agcagcaggc gatccaaggc cggcgtttcg
     5341 gcaagcagac gttttttgat aatggaggta tccccatttg tctgcgcctc ggctgacatg
     5401 gaacgcaagc gcttttcgat ccgcttcgcc tcggggcctt tgtaggcgtt ccaggcgagg
     5461 taacttcctt ccaggaacag cacgaccgcg atgaaggcga gaatgacgaa caaatagtag
     5521 agatagtcca tgctgggctc ctattcgtac aaccgggtgg ggtcgaaaat atcgtcggaa
     5581 atattgacgc cacgggtatg caatagatcg atgaacctgg gtcggatacc ggtggcgtgg
     5641 aaatggccac gcaccgttcc atcagaagcc acaccggtct gctcgaaaac aaacacatcc
     5701 tgcatggtaa cgatatcgcc ctccatgccg gtgatctcct gaatgctggt gatcttgcgc
     5761 ttgccatcgg atagccggga cacatgaatt atcgccgtaa tggcagaact gatttgctgg
     5821 cgcatcgcct tggtcggcaa attcaaccct gccatcccga ccatattttc cagacgggtt
     5881 aacgagtcac gcgggctatt ggcatgaacc gtggtcatcg aaccctcgtg accggtattc
     5941 atcgcctgga gcatatccag cgcctcggca ccgcgtatct cgccgaggat gatgcgatcc
     6001 gggcgcatgc gcaaactgtt tttcaccagg gcccgttgag cgacttcacc cttgccctcg
     6061 atgttgggcg gccgggtttc cagacgcacc acatggggct gctgcaactg gagttccgcc
     6121 gcatcctcga tggtgatgat gcgttcggca cggggaatga acccggacat gatgttcagc
     6181 agcgtggtct tgccggttcc cgttccgccg gaaatcaaca gattcacttt cgccttgacc
     6241 agccccccga tgatttccgc catctcaggc gtcaggctct tgccacggat aatgtcatcc
     6301 atgcccaacg gggtgacgga aaagcggcgg atggacagaa tcggcccgtc tatcgccagc
     6361 ggcggaatga tcgcattcac ccgcgagccg tcccgcagcc gtgcgtccac catcgggctg
     6421 gattcgtcga cgcgtcggcc gactcccgat acgatcttgt cgatgatctt catcaaatga
     6481 gtatcgtcct caaagcgcac atcggtcagc tccagtttac cggcgcgctc cacaaagacc
     6541 tgacgatagg tattggccag aatatccgaa acggtcggat ccgccagcag cgtctcgatc
     6601 ggccccaatc ccagcacctc gtgctgaata tcccgcacca gatttttgcg ttcggtgtca
     6661 ttgattgcca gcgtctcttc cgtaagcagg cgctccacca gcaccttgag ttcctcctgc
     6721 agcctgtcct gcgacagact ttccatgacg gacaggtcga cgcggtcaag cagcttttgg
     6781 tggatgcgcg atttcagctc ctgataggag cgattttcag ctgccgtcac ctgcggcgtc
     6841 accacgggca tctgcgtgcc gtcatgtttg atgttttcaa gtcgatctcg taatgacatg
     6901 gttcatcccc tccttcgttt ccggcgcatc aaaccgcctg cgtcggtaaa aaaaatcaca
     6961 atttaaaaat ccgggtaacg ctgcattgat tcaaatcaac cccacaaaaa acgaatccag
     7021 cccatcaagc gcgactgaac agacgtccaa accaacccgt tttctcttct tcgcccagtt
     7081 caaggtcatg cccgagtttg agcagtattt ttgcaaccgg gtcagcgcgg gccattttca
     7141 gaataggtac gccctgattg accgatgccg agaccgccac gtaactgttg ggaaaagtga
     7201 gcatgacctt catgcccaag gtgcgctcca catccgccaa ctggatgtcc cccccctttt
     7261 cgtaacggtt aacaatcagg tgaatcttgt ccttgctgta atccaaagat tgcagtgtgg
     7321 aaacgatgcg ttttgcgtcc cgaacaaagg gcaaggtttc ctgcaatacg ggaaagatca
     7381 gatcggcatg atcaagggcc ttgatgctca cggcctccag atttcgacct atgtccagga
     7441 tcacatagtc atattcggcc acggccaaac gcatcagcac ttcgatgtgc gatggccaga
     7501 catccgctgc cttggcagga ttttccggcg cggccagtac gccaaaattc ggcagcacct
     7561 tcaccatgct ggatgcgaat aaagaagcgt ccagacgaga aatattgccg accacatcgg
     7621 acagcgtaat cgtgggcacc ttgtcagaaa cgaacaagga tgcatcgccg aattgcagat
     7681 taagatcgat cagcgcgact cgcttgccgt attcggtcgc caatacatag cccagattgc
     7741 tggccagaaa agtggcgccg ctgccgccct tgcaggcgat gaaggccagc accttgcctt
     7801 tgtgcaaggg catctccgta cgcgcatgct tctgctcgac acgggcgacc gcctcgacca
     7861 atgcctgacg agcaaccgga accggcaaca catcgcgcac gccgagatgc atcaactgga
     7921 tcaaattttc cgccgaaact tgctcgcaca gtacgatgaa gctcatcccg gcatggcgct
     7981 gactcacccg ccccagtgca gcaatcgccc cctgtccatc gccagcgctt tcgatgacga
     8041 tcaggtctgg caactgctga tcggcgacgg cctccaattg ctcttcgctc ccatcgaagg
     8101 ataaaacaac gtgaagctgc tccccctcct gcaggatatg gacaaccccc tccctctggg
     8161 atttgttcgg tgataggacg gcgattttca cgttatgcct cgtttttatt tatctgtttc
     8221 gctacacatc agatcaactc cactcagtct gtggcaatca gacctcgcac ccagtgcgca
     8281 acaaacccaa actctcggtc ggcatcacca cggtcgaggg aggaaagggg ataggcggca
     8341 aaggaaacca gagcagcgtt cctatcagcg gagtgaaagt ggcattgacc ttaaccctga
     8401 caaaaccgat acagttctgc ggcgtaccca caccatcagg cttcatgcag ttactcgcat
     8461 tttcatccac gctgtccggc aagtcattgc ccgtcaaaac caccatatcg gctctgcgca
     8521 agggctctat ggatatattc gcagttgaaa gttccggggc cgcaggcagc gatgacagcc
     8581 ccatcaaccc tctggaattt atcatcgtat tccaatcgct ccgccagcag gttaccgact
     8641 ccctggcgac gtaacgcgtc acctcctgaa tggaattcca tacatagagt ccgcgcccaa
     8701 actcgacaat cccgaagagc aggccgaaga agataatggc aatcagcgca aactctaccg
     8761 tggcggcccc tcgctgtttt acactgaaat ccctcgcaaa aatcctcacg gtcagccccc
     8821 catataacgc atggtggtgt agggctggat accgaggtta tagccggcgc ttccaccgga
     8881 ataactcagt ggcaaccatt gccccaacct gatgccgtaa tcgacgatgg cgacggtcac
     8941 gtggatcgag ctcaacacgg cctggttgct cgaacagatg gaaccatcgc aacgcacctc
     9001 gactttcgtc gcatcgaaat cggtcacccc tgaatccgtg gcggccgttg ccaccatgct
     9061 tctggcattg gtcaccacgc tacccaatct gctgttgcag acatccaccg tttctc
//
LOCUS       DASUIV010000031         5520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_937062, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000031 DASUIV010000000
VERSION     DASUIV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937062"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1771
                     /locus_tag="VFW59_01030"
     CDS             260..1771
                     /locus_tag="VFW59_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HEX5362829.1"
                     /translation="MPSIVLATLNARYIHASLGLRYLAANMGELEADTEIMEFILQSR
                     PEEIVEALLAARPRIIGFGVYIWNVTEITRVVALLKCVAPQITVVLGGPEVSYECNEQ
                     EVVRLADYVITGWGDVSFPKLCRELLLQRPQAQKIIAGIQPKLAEIKLPYALYSDHDI
                     AHRTLYVEASRGCPFKCEFCLSSLDKTAQAFDLDLILDEMDKLYRRGARQFKFVDRTF
                     NLNIKTGARILEFFLERLDDKLFVHFEVIPDHLPEQLKALIAKFPPGSLQFEVGIQTL
                     NPEVQALISRKQDNAKTEENLRWLHQESHAHVHVDLIIGLPGEDVASIANGFDRLVAL
                     KQQEIQVGVLKRLRGTPIIRHTEAYAMRYTPYPPYTILATDRIDFATMQRLVRFARYW
                     DLVGNSGRFPQALELLLDANPFARFLAFSDWIYATTHQTHQIPLHKLFDLLYTGLTTK
                     LAVPIETARTALEQDFIQSGCKELPPFMKTEKDLPRKAKTGNLAKRQVRHLET"
     gene            complement(1819..3315)
                     /gene="lnt"
                     /locus_tag="VFW59_01035"
     CDS             complement(1819..3315)
                     /gene="lnt"
                     /locus_tag="VFW59_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX5362830.1"
                     /translation="MSQFRKLSLAAFAAGLLCVAGFAPFGWFPVPVLALAVLFALWAK
                     AATPRAAAWQGFAFGLGLFAGGISWIYVALHDYGEMPLPLAALATLLFAAFLALLPAL
                     AGYVQARVMPRSSSFVALVLSMPAAWVLVEWVRGMLFTGFPWLTLGYAHSDSPLAGYA
                     SLLGVYGVSLVAAASAGLLAMLWRERWNRRGQLALALLVVLWGCGAALRSIVWTQPQG
                     EPFSVALVQGNIAQDLKFNEDALVSTLETYRRLTLQSDARLIVLPETALPMLRHEVPG
                     QLVAQFAAHAQRNAGGVLIGSFVREGDAFHNSVFALTGEGREQRYHKQHLVPFGEFIP
                     LRLVLGWFINEVLNIPMGDLTSGGTHQPPLEVAGQRVAVNICYEDVFGEEIIRALPQA
                     TLLVNVTNDAWYGDSFAAEQHAQISRMRALETGRMMLRATNTGVTAIIGVDGRVLQQL
                     PQHHEGVLSGMAQGYAGSTPYVRWGNGAVMALLLGMLGVAWRRRTAMP"
     gene            3419..4150
                     /locus_tag="VFW59_01040"
     CDS             3419..4150
                     /locus_tag="VFW59_01040"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5362831.1"
                     /translation="MEKIRLSKRMSELGLCSRREADSYIEKGWVQVDGETVAELGSKV
                     YPHQQITLRTAAQNTQQQRVTILINKPMGYVSGQAEHDYKPASSLLDESSHWSEDESP
                     LKFHRSQLLGLAPAGRLDIDSTGLLVLTQDGRIAKRLIGEDSPVDKEYLVRVQGTLKD
                     GGLAQLCHGLKLDGEYLKPAKVTWQNEDQLRFVLREGKKRQIRRMCELVGLKVTGLKR
                     IRIGKVKLGNLPVGKWRYLGENEQF"
     gene            complement(4158..4997)
                     /locus_tag="VFW59_01045"
     CDS             complement(4158..4997)
                     /locus_tag="VFW59_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX5362832.1"
                     /translation="MDSSESSKPSLLERLSALLLREPEDREQLVRLLHSAYEHNLLDA
                     DALSMMEGVLQVSERQVREIMIPRAQMDVIDISVAPEQFIPYVIETAHSRFPVIDGDK
                     DNIIGILLAKDLLRYYAGEEFDVRDMLRPVVFVPESKRLNVLLREFRSNRNHIALVVN
                     EYGGVSGMVTIEDVLEQIVGDIEDEYDFDEDEDNIIPDDAGKWRVKADTEIDDFNEAL
                     GSEFSDEECDTVGGLVLKAFGQLPKHGDHIHIGELNFTVLRVDSRRLYSLLVERKKTD
                     APV"
     gene            complement(5006..5479)
                     /gene="ybeY"
                     /locus_tag="VFW59_01050"
     CDS             complement(5006..5479)
                     /gene="ybeY"
                     /locus_tag="VFW59_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5362833.1"
                     /translation="MRKTSRLAPPKLSLTVQYVSDAPNLPTRSQFRRWVKTALQRDVT
                     LTLRIVDEAEGQELNRGFRGKDYPTNVLTFVYDEDAAPGEMIYGDVVVCAPVVAREAR
                     EQGKDLLAHYAHMTIHALLHLQGFDHEIDAEAAEMEALETRLMLELRYPDPYQET"
BASE COUNT         1141 a         1772 c         1613 g          994 t
ORIGIN      
        1 tgtactcaag tctagcggaa cttggcttac gagcatggag gtgtgttacc taggaatgct
       61 cttgggccgt cttccgagag cagacattta aattgtctgt acatattttc caaggtgacc
      121 gctttggccg agttccagtc cgtcgttatt tcggtcgtgt agtgatagcg gtcttgccgc
      181 atgcatcgcg cggcctgctc gcaacacgag atttctggaa gcgctgtaac atgtcggcct
      241 ttcgatacac ctgacccgta tgccctccat cgtcctcgcc acgctcaacg cgcgctacat
      301 ccatgcctcg ctgggcttgc gctatctcgc cgccaacatg ggcgagctgg aagcagacac
      361 cgagatcatg gagttcatcc tgcagagccg gcccgaggag attgtcgagg ctttgctggc
      421 tgcccggccg cgcatcatcg gtttcggcgt atacatctgg aacgtgaccg agatcacccg
      481 cgtggtcgcc ttgctcaaat gcgtcgcccc gcaaatcacc gtggtgctgg gcgggcctga
      541 agtcagttac gaatgcaatg agcaggaggt cgtgcgcctg gccgattacg tgatcacggg
      601 ctggggagac gtcagctttc ccaaactttg ccgcgaactc ctgctgcagc gcccgcaggc
      661 gcagaagatc atcgccggca tccagcctaa gcttgccgag atcaagctgc cctatgcgct
      721 ttactcggat cacgacatcg cacaccgcac gctgtatgtg gaagcgtcgc gcggctgccc
      781 gttcaaatgc gaattctgcc tctcgtcatt ggacaagacg gcacaggcct tcgatctcga
      841 tctcatcctc gacgagatgg acaagctgta tcggcgcggc gcccgtcagt tcaaatttgt
      901 cgaccgcacc tttaacctca acatcaagac cggcgcgcgc attctggaat ttttcctcga
      961 acgcctggac gacaaactgt tcgtgcactt cgaggtcatc cccgatcacc tgccggagca
     1021 gctgaaagcg ctgatcgcca aattccctcc cggctcgctg caattcgagg tgggcatcca
     1081 gaccctgaac ccggaagtgc aggcgctgat ctcgcgcaaa caggacaacg ccaaaacaga
     1141 agagaacctg cgctggctgc atcaggaaag ccacgcgcat gtccacgtcg acctcatcat
     1201 cggcctgccc ggcgaggatg tggcgagcat cgccaacggc ttcgaccggc tggttgcgct
     1261 gaagcagcag gagatccagg tcggcgtgct caagcgcctg cgcggcacgc ccatcatccg
     1321 ccataccgag gcgtacgcca tgcgctacac cccctacccg ccctacacca tactcgccac
     1381 cgaccgcatc gactttgcca ccatgcaaag actggtgcgc tttgcccgct actgggatct
     1441 ggtgggcaac tccgggcgtt ttccgcaggc actggaactg ttgctggatg caaatccgtt
     1501 tgcacgcttc ctggctttct ccgactggat ctacgccacc acgcatcaga ctcatcagat
     1561 tccgctgcac aaactgtttg acctgctcta caccgggctg acgaccaagc ttgcagtgcc
     1621 gatagaaacg gcacgcacag cgctggagca ggattttatc cagtcaggct gcaaggagtt
     1681 gccgccgttc atgaaaaccg agaaggactt gccccgtaag gccaagacag gaaatctggc
     1741 gaaacgtcag gtgcggcacc tggagacctg atcgactccg attggcacag agcctgtcac
     1801 ccgcgattta ggagcccatc aaggcatggc agtgcgacga cgccaggcca cgcccagcat
     1861 ccccagcagc aacgccatca ccgccccgtt gccccagcgc acatagggcg tgctgcctgc
     1921 atagccctgc gccatccccg acaacacgcc ttcatgatgc tgcggcaact gctgcagcac
     1981 tcggccgtcc acgccgatga tcgcggtcac gccggtgttg gtggcgcgca acatcatgcg
     2041 gccggtctcc agtgcgcgca tgcgcgaaat ctgagcgtgc tgctcggcgg cgaaagagtc
     2101 gccgtaccac gcatcgttgg tgacgttcac cagcagcgtg gcctgcggca acgcgcggat
     2161 aatctcttcg ccgaacacgt cttcgtagca gatgttcacc gccacgcgct gaccggcgac
     2221 ttccagcgga ggctggtgcg tgccgccgct ggtcaggtcg cccatcggga tgttcagcac
     2281 ttcgttgatg aaccagccca gcaccaggcg cagggggatg aattcgccga acggcaccag
     2341 atgctgcttg tgataacgct gctcgcggcc ctccccggtc agggcgaaca cgctgttgtg
     2401 gaaggcatcg ccctcgcgca cgaaagagcc gatcagcacg ccgcccgcat tccgttgtgc
     2461 gtgggcggcg aactgtgcca ccagctggcc gggcacttcg tggcgcaaca tcggcaacgc
     2521 ggtctcgggc agcacgatca ggcgcgcatc gctttgcagt gtcaggcggc gataggtctc
     2581 cagcgtcgag accagcgcat cctcgttgaa cttgagatcc tgcgcgatgt tgccctgcac
     2641 cagcgccacg ctgaacggct cgccctgcgg ctgcgtccag acgatgctgc gcaacgccgc
     2701 gccgcagccc cacagcacaa cgagcagcgc cagggccagc tgtccgcgcc ggttccagcg
     2761 ctcgcgccac agcatggcca gcagccccgc gctcgccgcc gcgaccagcg atacgccgta
     2821 cacgccgagc agcgacgcat aaccggccaa cgggctgtcg ctatgcgcat aacctaacgt
     2881 cagccagggg aagccggtaa acagcatgcc gcgcacccac tccaccagca cccaggccgc
     2941 aggcatcgac agcaccagcg cgacgaaaga cgaggagcga ggcatgactc tggcctgcac
     3001 ataaccggcc agcgcgggaa gcagcgccag gaaggcggca aacaacaggg tcgccagcgc
     3061 agccaacggc agcggcatct cgccgtagtc gtgcagcgcg acgtagatcc agctgatgcc
     3121 gcccgcgaac aggccgagac cgaaggcgaa gccctgccat gccgcagcgc gcggcgtagc
     3181 ggctttggcc cacagcgcaa acagcacggc gagcgccagc accggcacag ggaaccagcc
     3241 aaatggcgca aaacccgcca cacacaatag ccctgccgcg aaggcagcaa gcgacaattt
     3301 tcgaaattgg gacatgaaac gactgatcac gaagaaggcg cgaagaataa ccgattccgt
     3361 ccggggcggc atttgtgctt aaatcgcgcc ctgatctctc gtgcacggca aaatacccat
     3421 ggaaaaaatc cgactctcaa aacgcatgtc cgaactcggc ctctgttcgc gccgcgaagc
     3481 cgacagttac atcgaaaaag gctgggtaca ggtggatggc gagaccgtag ccgaactcgg
     3541 cagcaaggtc tacccgcacc agcagatcac cctgcgcacc gccgcacaga acacccagca
     3601 gcagcgcgtc accatcctga tcaacaagcc tatgggctac gtgtcggggc aggccgagca
     3661 cgattacaaa cccgccagct cactgctgga tgagtcttcg cactggagcg aggacgagtc
     3721 gccgctgaaa ttccaccgca gccagctgct ggggctggcc ccggcaggcc gactggacat
     3781 cgactcgacc ggcctgctgg tgctgaccca ggacggacgc atcgccaaac ggttgatcgg
     3841 cgaagactcg ccggtggaca aggaatatct ggtgcgcgtg cagggcacgc tgaaagacgg
     3901 cggcctggcg cagctctgcc acggcctcaa gctggacggc gaatatctca aacccgcgaa
     3961 ggtgacctgg cagaacgagg atcagttgcg cttcgtgctg cgcgaaggca agaaacgcca
     4021 gatccgccgc atgtgcgaac tggtcggatt gaaggtgacc ggactgaagc gcatccgcat
     4081 cggcaaggtg aagctgggca atctgccggt aggaaagtgg cgctatctgg gcgagaacga
     4141 acagttctga ccttgcatta caccggcgcg tcggtcttct tgcgctccac cagcagggaa
     4201 tacagcctgc ggctatcgac gcgcaacacg gtgaagttca gttcgccgat atggatgtgg
     4261 tcgccgtgtt tgggtagctg accgaaagcc ttcagcacca gcccgcccac cgtatcgcat
     4321 tcctcgtcgc tgaattcgct gccgagcgcc tcgttgaaat cgtcgatctc ggtatcggcc
     4381 ttcacgcgcc atttgcccgc gtcgtccggg atgatgttgt cctcgtcctc gtcgaaatcg
     4441 tattcgtcct cgatgtcgcc gacgatctgc tccagcacgt cctcgatggt caccatgccg
     4501 gacacgccgc cgtactcgtt caccaccagc gcgatgtgat tgcggttgct gcggaactcg
     4561 cgcagcagca cgttgaggcg tttggattca ggcacgaaca ccaccggtcg cagcatgtcg
     4621 cgcacgtcga attcctcgcc cgcgtagtag cgcagcaggt ccttcgccag caggatgccg
     4681 atgatgttgt ccttgtcgcc gtcgatgacg gggaagcgcg agtgcgcggt ctcgatgacg
     4741 taggggatga actgttccgg cgccacactg atgtcgatga catccatctg cgcgcgcggg
     4801 atcatgatct cgcgcacctg gcgctcagac acctgcaaca cgccttccat catggatagc
     4861 gcatcggcat cgagcagatt gtgttcgtag gcggagtgca gcaagcggac gagttgctcg
     4921 cggtcttcgg gttcgcgcag caacagcgcg gaaaggcgtt ctaacagact gggtttacta
     4981 ctctcggatg aatccatttg gtgctttaag tttcctgata aggatcgggg tagcgcaatt
     5041 ctagcatcag ccgggtctcg agcgcttcca tctcggcggc ctcggcgtcg atctcgtggt
     5101 cgaagccttg cagatgcagc aatgcatgga tagtcatatg tgcgtaatga gcgagcaagt
     5161 ccttaccctg ctcacgcgcc tcacgcgcca ccacgggggc acagacaacg acatcgccat
     5221 aaatcatttc gccgggcgct gcgtcttcgt cgtaaacaaa ggtcaataca ttggtgggat
     5281 agtccttgcc gcgaaagcct cggttcagct cctgcccctc ggcctcgtcc acgatacgca
     5341 gcgtcagcgt cacatcgcgc tgcagtgcag tctttaccca acgacggaac tgcgaccgcg
     5401 taggcaggtt gggcgcatcg ctgacgtact gcacggtcag cgacaacttg ggcggcgcga
     5461 gcctggatgt ttttctcata gcattcatag aatggtcagc agtgcatgcg cagcggtgaa
//
LOCUS       DASUIV010000032         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_937069, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000032 DASUIV010000000
VERSION     DASUIV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937069"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1189
                     /locus_tag="VFW59_01055"
     CDS             164..1189
                     /locus_tag="VFW59_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5362834.1"
                     /translation="MKNVLIGGALLMGAVAVQAAEQLAVAPVQYREVAQTYSADGMVE
                     AVRQSTVSAQIGGRVKEILFDVGDRVARGQLILRIDEREASQALAGSQAQVLQAQANM
                     QNAKANYERAKQLFAQKFISQAGLDKAQADYKVALAQAAASEAGAGQAGIAHGYTSVM
                     APYGGVVASRLVEVGEMVMPGKPLMTGFDPSEMRVVVSVPQYKLADIGKHPEAAVEIP
                     TLKRWIDAASVTVQPLADARTHSTQVRVNLPANEPGIYPGMFVRTHFVVGKVKKLLVP
                     AGAVLRRSEVVAVYVVDDKGDAKLRQVRVGDMAADGFIEVLAGLNDGEKVALEPVKAG
                     MLGAKRK"
     gene            1192..>3890
                     /locus_tag="VFW59_01060"
     CDS             1192..>3890
                     /locus_tag="VFW59_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5362835.1"
                     /translation="MSMGISGRIAKLFLHSQMTPLLALVAVLLGLFAVAVTPREEEPQ
                     INVTMANVLIPYPGASAKDVAQTVATPAEQVLAQIAGVDHVYSVSQPGMAVITVQFKV
                     GEQHVPSLVKLYDVVHSHVDWLPTTLGVSQPIIKAKGIDDVPIVALTLWREQDAGGGI
                     ELTQVAHAIEAELKRVPGTREVNTLGASQRMVRVLLSPENLNAYQLTMQDVRAALQSA
                     NVSQNSGNLVQDNREVLVQTGSFLRDASEVRQLVIGVSDGKPVFLRDVAEIKDGADQP
                     GSYVWLGTGPAADDKQIKARGEFPAVTVAVTKKPGANAVEIADKLMQRVNELKGSVIP
                     DDVQVSVTRNYGETANDKAMKLIKKLLFATAAVVALVWFTMGRREALVVGVAVLLTLA
                     ATLFASWAYGFTLNRVSLFALIFSIGILVDDAIVVVENIHRRRALAQHQPLSEIIPEA
                     VDEVGGPTILATFTVIAALLPMAFVSGLMGPYMSPIPINASMGMLISLAIAFVITPWL
                     VYRFAGAQHEIVHNEEETGLNRFFRMRLGPFLNREHGKGARRKLWLGILAALMFAVSL
                     AVVKLVVLKMLPFDNKSEFQIVVDMPSGTALEQTNAVLHDIGDYLATVPEVTDYQAYA
                     GAASPINFNGLVRQYYLRSDAEQGDVQVNLRDKHNRSRSSHQIASSALEPIQKIAQRW
                     GAKIKIVEVPPGPPVMSPIVAEIYGPDYEGSRAVAGKVREQFGKTESIVGIDDSVSEA
                     APKLLLRVLQSKAALLGVAPHDIVDVIGMALTGQDVASLHDENAKYAPPLRIGLPAER
                     RSQIDEVLKLKVRARDGGLVPLSELVQVVPAERDYPIYHKDLLPVVYVFGDMAGKLDS
                     PLYGMFDINGKTSGAALEQGGVLESYFFKQPSDPYAGYALKWDG"
BASE COUNT          703 a         1078 c         1379 g          730 t
ORIGIN      
        1 cgcaaggatg ccaaccgggt gttctatcgc atcagcgatt tgcgcacgct caagctggtg
       61 gtgatgatgc gcgaggtgtt ctgcacggtt taggacattg tttttttatg tctattagga
      121 tattctaata gatgtaatta attattcgga ataaggtggc ctgatgaaaa atgtcctgat
      181 cggcggtgcg ttgttgatgg gagctgtcgc tgtgcaggcg gcagagcagc tcgccgtcgc
      241 cccggtgcaa tatcgcgagg ttgcgcagac ctatagcgcc gacggcatgg tcgaggcggt
      301 gcgtcagtcc acggtgtccg cgcagatcgg cggtcgcgtg aaggaaattc tgttcgatgt
      361 cggcgaccgt gtggcgcggg ggcagctgat tctgcgcatc gacgagcgcg aggcgtcgca
      421 ggcgctggcc ggaagtcagg ctcaggtatt gcaggcgcag gccaacatgc agaacgccaa
      481 ggctaactat gagcgcgcca agcaactgtt cgcacagaaa ttcatcagcc aggcaggact
      541 ggacaaggcg caggccgatt acaaggtggc gctggcacag gctgccgcca gcgaggctgg
      601 ggccgggcag gccggcatcg ctcatggcta cacctcggtc atggcgcctt atggcggcgt
      661 ggtcgcgtcg cgtctggtcg aggtgggcga gatggtgatg ccgggcaagc cgctaatgac
      721 cggattcgat ccctcagaga tgcgcgtggt ggtcagtgtg cctcaataca aattggccga
      781 tatcggcaag catcccgagg cggcggtgga gattcctacg ctgaagcgct ggatcgatgc
      841 ggcttcggtg acggtgcagc cgctggccga tgcgcgtacc catagcacgc aggtgagggt
      901 gaatctgccg gccaatgagc cgggcatcta tccgggcatg ttcgtgcgta cccatttcgt
      961 ggttggcaag gtgaagaagc tgctggttcc tgccggtgcg gtgctgcgcc gcagcgaggt
     1021 cgtggccgtg tatgtggttg acgacaaggg cgatgcgaag ctgcgccagg tgcgcgtcgg
     1081 cgacatggcc gcagacggct ttatcgaggt gctggccggg ctgaatgacg gcgagaaggt
     1141 ggcgctggag ccggtcaagg ccggcatgct cggcgcgaag aggaaataga tatgtccatg
     1201 ggaatctccg ggcgtatcgc caagctgttc ctgcattcgc agatgacgcc gctgttggcg
     1261 ctggtcgcgg tgctgctggg tctgttcgcc gtggcggtca cgccgcgcga ggaggagccg
     1321 cagatcaacg tgaccatggc gaatgtgctg attccctatc cgggggcatc ggccaaagac
     1381 gtggcgcaga ccgtggcgac gcccgccgag caggtgctgg cgcagatagc cggcgtggat
     1441 catgtgtatt cggtgtcgca gccgggcatg gcggtgatca cggtgcagtt caaggtgggc
     1501 gaacagcatg tgccttcgct ggtgaagctc tacgacgtgg tgcattccca cgtcgactgg
     1561 ttgccgacca cgctgggtgt atcccagccc atcatcaagg ccaagggtat cgacgacgtg
     1621 cctatcgtcg cgctgacgct gtggcgcgag caggacgcgg gcggcggtat cgaactgacg
     1681 caagtggccc atgccatcga ggccgaattg aagcgggtgc ccggcacgcg cgaggtgaat
     1741 acgctgggtg cctcgcagcg catggtgcgc gtgctgctga gcccggaaaa tctgaacgcc
     1801 tatcagctca cgatgcagga tgtgcgcgcc gcgttgcagt ccgccaatgt gtcgcagaac
     1861 tcgggaaatc tggtgcagga caaccgcgag gtgctggtgc agacgggcag cttcctgcgc
     1921 gatgccagcg aggtgcggca attggtgatc ggggtgtcgg acggcaagcc ggtgttcctg
     1981 cgcgatgtcg ccgagatcaa ggatggcgcg gatcagcccg gcagctatgt gtggctcggc
     2041 accggccccg ccgccgacga taagcagatc aaggccaggg gagagttccc cgccgtaacc
     2101 gtggcagtta ccaagaagcc gggtgcgaac gcggtcgaga tcgccgacaa gctgatgcag
     2161 cgcgtgaacg agctgaaggg cagcgtgatc ccggacgatg tgcaggtcag cgtgactcgc
     2221 aactatggcg agaccgccaa cgacaaggcg atgaagctga tcaagaagct gctgttcgcc
     2281 acggcggcgg tggtcgcgct ggtctggttc acgatggggc ggcgcgaggc gctggtggtg
     2341 ggggtggcgg tgttgctgac gctggcggcc acgctgttcg cctcctgggc ctacggcttt
     2401 acgctgaacc gggtgtcgct gttcgcgttg atcttctcca tcggcattct ggtggacgac
     2461 gccatcgtgg tggtggagaa catccaccgc cgccgcgcgc tggcgcagca tcagcccttg
     2521 tccgaaatca ttcccgaggc ggtggacgag gtcggcggcc cgaccatact cgcgactttc
     2581 acggtgatcg ccgcgctact gccgatggca ttcgtcagcg gactgatggg gccgtacatg
     2641 agcccgatcc cgatcaatgc cagcatgggc atgctgatct cgctggcgat cgccttcgtg
     2701 atcacgccct ggctggtcta tcgttttgcc ggtgcgcagc acgaaatcgt gcataacgaa
     2761 gaggagaccg ggctcaaccg tttcttccgc atgcgcctcg gcccgttcct gaatcgcgag
     2821 catggcaagg gcgcgcggcg caagctgtgg ctggggattc ttgcggcgct gatgttcgcc
     2881 gtgtcgctgg cggtggtgaa gctggtggtg ctgaagatgc tgccgttcga caacaagtcg
     2941 gaattccaga tcgtggtgga tatgcccagc ggtacggcgc tggagcagac caatgcggtg
     3001 ctgcacgata tcggcgacta tctggcgacc gtgccggagg tgaccgacta tcaggcctac
     3061 gcgggcgcgg cttcgccgat caacttcaac ggtctggtgc gccagtacta cctgcgcagc
     3121 gatgccgagc agggcgatgt tcaggtcaat ctgcgcgata agcacaatcg cagccgcagc
     3181 agtcaccaga tcgcctcgtc cgcgctggag ccgatccaga agatcgcgca gcgttggggg
     3241 gcaaagatca agatcgtgga agtgccgccc ggccctccgg tgatgtcgcc tatcgtggcg
     3301 gagatatatg gcccggacta tgagggctcg cgcgcggtgg ccggcaaggt gcgcgaacaa
     3361 ttcggcaaga ccgagagcat cgtcggtatc gacgacagcg tcagcgaggc cgcgcccaag
     3421 ctgttgttgc gcgtgctgca gagcaaggct gcattgctgg gcgtggcgcc gcacgacatc
     3481 gtggacgtga tcggtatggc gctgaccgga caggatgtcg cctcgctgca cgacgagaat
     3541 gcgaagtatg cgccaccgtt gcgcatcggc ctgcctgcgg agcggcgcag ccagatcgac
     3601 gaggtgctca agctcaaggt gcgcgcccgc gacggcgggc tggtaccgtt gtccgagctg
     3661 gtgcaggtgg tgcctgccga gcgcgactat ccgatctatc acaaggattt gttgccggtg
     3721 gtgtacgtgt tcggcgacat ggcgggcaag ctggacagcc cgctgtacgg catgttcgac
     3781 atcaacggca agaccagcgg cgcagcgctg gaacagggcg gtgtgctgga gagctatttc
     3841 ttcaagcagc cttccgatcc ctatgccggt tatgcgctga agtgggacgg
//
LOCUS       DASUIV010000033         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_472331, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000033 DASUIV010000000
VERSION     DASUIV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472331"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFW59_01065"
     CDS             complement(<1..1326)
                     /locus_tag="VFW59_01065"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5362836.1"
                     /translation="MLNTARATHAIAAAPHHLASQSALAVMRDGGNAIEAMVAAAATI
                     AVVYPHMNGIGGDSFWLIVPPGGAPIAIDACGQAASAATIDAYRSRGLSAIPVRGPLA
                     ANTVAGTVGGWHTALEVAAELGGKLPLARLLADAIGYAQEGVPVTVSQQNATRAKLPE
                     LAAQPGFSDTFLIDGALPLAGQRFKQPRMARTLSLLLRDGLDSFYRGSLAAEIAADLQ
                     TVDTLLTGSDLEKYHAQRKTPLQLAHSAGEVYNMPPPTQGAVSLAILGLLDRVGLSAV
                     EADSADHVHLVVEATKLAFQLRDQYITDPADMTVDPQALLAPAPLDALAANIRLDQAA
                     PWGSGRGPGDTIWMGTIDQSGLAVSFIQSIYHEFGSGVVLPATGINWQNRGASFSLDA
                     DKLLALKPGKKPFHTLNPAAARLKDGRTMVYGTMGGDGQPQTQAAVFTRH"
     gene            complement(1367..2065)
                     /locus_tag="VFW59_01070"
     CDS             complement(1367..2065)
                     /locus_tag="VFW59_01070"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5362837.1"
                     /translation="MNQPVPNTVGAAERLRGGRADAICKKIADEIVLGRFEPGSRLDE
                     VMLARLFSVSRTPVREALKQLAIQGLVVCRPNRGAVVAKMTPEQLDRMFEAIGELEAA
                     CARHAAIRMTETNRDHLCSLHAQCRVAMRAGDFERYDRLNQELHHMVILGSCNPVLIE
                     ITLSLRHRVSPFMRSQFRDVERMSASFEEHAVIVEALLAHDVTTAHREMRAHLFSARS
                     AWARMSSGWMNAPM"
     gene            complement(2062..3042)
                     /locus_tag="VFW59_01075"
     CDS             complement(2062..3042)
                     /locus_tag="VFW59_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5362838.1"
                     /translation="MSTPLVSVKDLTVEFHGSRCASALNQVSFDLQAGEVLGLLGESG
                     SGKSVTLRTLLKLHPQRSTRISGQIQIDGLDVLALQGRALETYRGGVASMVFQEPGLA
                     FDPVYTIGQQITEAIRAHESISATSARSQALQMLERVQIPQAKRRFDAYPHELSGGMR
                     QRAMIALALACKPKLLLADEPTTALDATVQIQILLLLRELQRETGMAVIFVTHDIGAA
                     VEVADRIAVMYAGRIVEENSVAGMVRGARHPYSAGLLASTVSSENRGQPLKAIPGTPP
                     DLAALPAGCSFAPRCAHSSAQCRVARPQVVNIGDTRLACWHPLLPLADAA"
     gene            complement(3039..>3576)
                     /locus_tag="VFW59_01080"
     CDS             complement(3039..>3576)
                     /locus_tag="VFW59_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5362839.1"
                     /translation="RQRVNIARALAFNPRLVILDEAVAALDKSVQAQVLNLLQELKAE
                     HQLTYLFISHDLHVVHYICDEVMVMYLGQVVEIGPVEQIYGHAAHPYTRALLSAVPSM
                     DPAHRTEQPPLTGDPPNPINPPAGCRFRERCPHAESVCAARSPALVPAAQLAGHLVAC
                     HMNDPRSGHTAAVMENAA"
BASE COUNT          690 a         1098 c         1106 g          682 t
ORIGIN      
        1 atgccgggtg aagactgctg cctgcgtttg cggttgaccg tcgccgccca tcgtgccata
       61 caccatggtg cggccatcct tgagacgcgc tgcggctgga ttcaatgtgt gaaatggttt
      121 ctttccaggt ttcagcgcca gcaatttgtc cgcatccaga ctgaacgacg cgccgcgatt
      181 ttgccagttg atgccagtgg caggcagtac aacgccgctc ccgaattcgt gataaatact
      241 ttgaataaag gacacagcca agccgctctg atcgatggtt cccatccaga ttgtgtcgcc
      301 tgggccacgg ccgctacccc acggtgcggc ctgatcgaga cggatgttcg ccgccagcgc
      361 atcgagcggc gccggcgcca gcagcgcctg tggatcgacc gtcatgtcgg ctgggtcggt
      421 gatgtactgg tcgcgtagtt gaaaggcgag cttggtcgct tcgaccacca gatgtacatg
      481 gtcggcgcta tcggcttcga ccgccgacaa gccgacgcga tcgagcaggc cgagaatcgc
      541 cagcgagacg gcaccttgtg ttggcggtgg catgttgtac acctcgcctg cggaatgcgc
      601 gagctgcaat ggtgtcttgc gttgcgcatg atatttttcc agatccgagc cggtgagcag
      661 ggtatcgacc gtctgcagat cggcggcgat ctcggctgcc agcgaaccac gataaaaact
      721 gtccaggcca tcgcgcagca acagcgataa ggtacgcgcc atgcgcggct gtttgaagcg
      781 ctggccggcg agcggcaatg caccatcgat caggaaggtg tcgctgaaac ccggttgcgc
      841 ggccaactcc ggcagtttgg cacgggtcgc attttgctga ctgacggtga ccggtacgcc
      901 ttcctgtgca tagccgattg catcggccag cagacgcgcc agcggcaact tgccgcccag
      961 ttccgcagcg acttcaagcg ccgtgtgcca gccgccgacg gtgcccgcta ccgtgttggc
     1021 agcgagcggc ccgcgcactg gaatcgctga caaaccccgg ctgcgatagg catcgatcgt
     1081 ggcggctgat gccgcctgcc cgcaggcgtc gatggcaatc ggcgcgccgc caggcggtac
     1141 gatgagccag aatgaatcgc cgccaatacc gttcatgtgc gggtagacca ccgcgatggt
     1201 cgcggctgct gcgaccatcg cttcaatcgc attgccaccg tcgcgcatca cggccagggc
     1261 cgactgggat gccagatggt gtggcgcggc cgcgatggcg tgggttgcgc gtgctgtgtt
     1321 caacatagat ttccttcggt tgagagatca ataagggaga taacgatcac attggcgcat
     1381 tcatccaacc cgacgacata cgcgcccagg cgcttcgggc cgaaaacaag tgcgcgcgca
     1441 tttcgcggtg tgcagtggtc acgtcatggg ccagcaatgc ctcgacgatg acagcatgct
     1501 cttcaaatga cgcgctcatg cgttcgacat cgcggaactg cgatcgcatg aatggcgaga
     1561 cccgatggcg caaactcaag gttatctcaa tcaggacagg attgcaggaa cccagaatga
     1621 ccatgtgatg caattcctga ttgaggcgat cgtagcgctc aaaatcgcca gcccgcatcg
     1681 cgacgcgaca ctgggcgtgt aagctgcata agtgatcacg attggtttcc gtcatgcgga
     1741 tcgccgcgtg acgggcacag gccgcttcga gttcgccaat ggcctcaaac atcctgtcga
     1801 gctgttcggg cgtcatcttt gccaccacgg caccacgatt gggtcgacag acgaccagtc
     1861 cctggatggc cagctgtttc agcgcttccc gcactggcgt gcgcgagacg ctgaacagcc
     1921 tggccaacat cacctcgtcg agccgcgagc cgggttcgaa gcggccgagc actatttcat
     1981 ccgcaatttt tttacaaatc gcatcggcgc gtccgccacg caggcgttcc gctgcaccca
     2041 ccgtgttcgg cacgggctga ttcatgccgc atccgccagc ggcagcagcg gatgccagca
     2101 ggcgagccgt gtgtcgccga tgttgaccac ctgcgggcgc gcaacgcggc actgcgcgct
     2161 ggaatgcgcg caacgcggcg cgaagctgca gccggccggc agcgcggcaa ggtcgggcgg
     2221 ggtaccgggg atcgccttca atggctggcc gcgattttcg ctgctaacgg tcgaggccag
     2281 caagccggcg ctgtagggat ggcgagcacc tcgcaccatg ccagccactg aattttcttc
     2341 aacgatacgg ccggcataca tcactgcaat ccgatcagcg acttcgacgg ctgcaccgat
     2401 atcgtgcgtg acaaaaataa cggccatgcc ggtctcacgc tgcagttcgc gcagcagcag
     2461 caatatctga atctgcaccg tcgcatccag cgcggtcgtt ggctcatcgg ccagcaacag
     2521 ttttggtttg caggccagcg cgagcgcgat catcgcgcgt tgtcgcatgc cgccggagag
     2581 ttcatgcgga taggcgtcaa agcgacgttt ggcttgcggt atctgtaccc gctccagcat
     2641 ttgcaatgcc tggctccggg cgctggttgc gctgattgat tcatgcgcac ggatcgcttc
     2701 ggtgatctgt tggccgatgg tgtacaccgg atcaaaggcc agacccggtt cctgaaatac
     2761 catcgaggcg acaccacccc tataggtttc cagagcccgt ccttgcagag ccagcacatc
     2821 caggccatcg atctgaatct gaccgctgat ccgggtgctg cgctgcggat gcagcttcag
     2881 caaagtgcgc aatgtgacgc tcttgcccga gcctgattcg cccagcaagc ccaatacttc
     2941 tccggcctgc aaatcgaagc tgacctgatt cagcgcgctg gcgcagcggc tgccatggaa
     3001 ttcgaccgtc agatctttca ccgacaccaa aggcgtactc atgcggcgtt ctccatgacg
     3061 gcggcggtat ggcctgagcg tggatcattc atgtggcagg cgaccaggtg gccagctagc
     3121 tgggcggcag gcaccagtgc cggcgaccgc gcggcgcaga ccgactcggc atgtggacaa
     3181 cgctcgcgaa agcggcaacc ggcgggcggg ttgatcgggt tcggcggatc gccggtcagc
     3241 ggtggctgtt cggtacggtg cgccgggtcc atcgagggca cggctgacag caatgcgcgg
     3301 gtgtagggat gcgccgcatg accgtaaatt tgttcgaccg gcccgatctc gaccacttgg
     3361 ccgagataca tcaccatcac ctcgtcgcaa atgtaatgca ctacatgcag gtcgtgcgaa
     3421 atgaacagat aggtcagctg atgttccgcc ttcagctcct gcagcaggtt gagcacctgc
     3481 gcttgcaccg acttgtccag tgccgcgacc gcctcatcca gaatcaccag gcgcggattg
     3541 aacgccagtg cgcgggcgat attgacgcgc tggcgc
//
LOCUS       DASUIV010000034         6878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_472392, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000034 DASUIV010000000
VERSION     DASUIV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472392"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /gene="lpdA"
                     /locus_tag="VFW59_01085"
     CDS             complement(<1..1480)
                     /gene="lpdA"
                     /locus_tag="VFW59_01085"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5362840.1"
                     /translation="MSQIVEIKVPDIGGFKDAAIIEIAVKVGEQIEAEQTLITLETDK
                     ATMDVPTPTAGVVKELKVKVGDKVSEGTLILMLESSAAVAAPASAPAPQTAPTAAPQA
                     AAPQAAPTPAAKVAKGDIHAEVVVLGAGPGGYTAAFRAADLGKQVVLIEKHASLGGVC
                     LNVGCIPSKALLHVAKVISEAEEVSHHGVTFAKPKIDIDAIRSWKESVITKLTGGLAG
                     LAKQRKVQVVRGTAKFTSPNSIAVQTEEGEKIVTFDNAIVAAGSSVARIPGFPYDDER
                     IIDSTGALALKDVPKRMLVIGGGIIGLEMATVYDALGAKISVVELMDQLMPGADKDMV
                     KPLHTRIAKRYEAIMLKTKVTKIEAQKSGLKVTFEGEQAPAEPQLYDRVLMAVGRRPN
                     GREINAEAAGLIVNERGFIPVDKQMRTNVPHIFAIGDIVGDPMLAHKAVHEGKVAAEN
                     IAGHKAFFEPLTIPSVAYTDPEIAWMGLTETQAKAQGIAYEKG"
     gene            complement(1506..2765)
                     /locus_tag="VFW59_01090"
     CDS             complement(1506..2765)
                     /locus_tag="VFW59_01090"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX5362841.1"
                     /translation="MEEIKQYMKTVGQQARAASRLMAQADTAAKNRALTAIAEAILND
                     SARLIAENAKDVAAAKANGLDAASVDRLTLTEKTVQGMAEGLRQIATLPDLIGSISDM
                     SFRPSGIQVGRMRVPLGVIGIIYESRPNVTADAAGLCIKSGNACILRGGSEAIHSNQA
                     IAACVHAGLKAAGLPETAVQVVTTTDRAAVGELITMKEYVDVIVPRGGKSLIARISDE
                     ARVPVIKHLDGICHVYIDDEADLTKAIRIADNAKTHRYGVCNAMETLLVNEKVAAQVL
                     PELSKIYLDKGVELRGDDAARAIVSQMTAATIDDWRTEYLAPILSVRVVANLDEAIDH
                     INTYSSQHTDSIVTENYTKAMRFLREVDSSSVMVNASTRFADGFEYGLGAEIGISTDK
                     IHARGPVGLEGLTSQKYIVLGSGQIRV"
     gene            2847..3650
                     /gene="trpC"
                     /locus_tag="VFW59_01095"
     CDS             2847..3650
                     /gene="trpC"
                     /locus_tag="VFW59_01095"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX5362842.1"
                     /translation="MSDILKKILAVKAQEVAAAKPVKPLPQLRAEAEQMPPARDFVGA
                     IRTMVEAGRSAVIAEIKKASPSKGVIREDFRPAEIAQSYAQHGAACLSVLTDEQFFQG
                     SAEYLQQARAACDLPVLRKDFMVDEYQIWQARAMGADAILLIAAALTLSQMKAFETLA
                     HKLGMAVLVEVHDEAELETALQLSTPLIGINNRNLRTFEVSLQTTLDLLVHIEESKKG
                     RGRIVVTESGIFTAQDVKLMRDHDVHGFLVGEAFMRADDPGAELAKVFS"
     gene            3662..4288
                     /gene="coq7"
                     /locus_tag="VFW59_01100"
     CDS             3662..4288
                     /gene="coq7"
                     /locus_tag="VFW59_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEX5362843.1"
                     /translation="MLNLDQVIVEFDKGLRTLFSQAHSARPHPDANVPDAPMDEAERK
                     HAAGLMRINHSGEVCAQALYQGQALTARDPAVQLKLQYAAQEETEHLAWTSKRVHELG
                     GHLSVLNPLWYAGSLTLGACAGLLGDKWNLGFLSETEHQVGGHLQSHLDELPAQDAKS
                     RAVVQQMYLDETQHSEMAKEMGGAELPEPVKLVMQATSKILTQAAYRI"
     gene            complement(4337..4684)
                     /locus_tag="VFW59_01105"
     CDS             complement(4337..4684)
                     /locus_tag="VFW59_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5362844.1"
                     /translation="MHTYTASLFSRRMKTARQLSGLSQMQLGIRAGIDASSASSRINQ
                     YERGKHAPDFLTVCNLAKVLCVPSAYFYAEEDALAELISLFGQMKAAERKLLLACADS
                     LAKEVAAAQGGEE"
     gene            4834..5106
                     /locus_tag="VFW59_01110"
     CDS             4834..5106
                     /locus_tag="VFW59_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362845.1"
                     /translation="MQEFAKFYDSLHVLVSDGEVKAAIREEFFRLGLVANVERFFGLF
                     GKDKRYFLVRFATTDDAIRTANQYKLRSFGFYGVLIELRGEHGSPQ"
     gene            complement(5306..5722)
                     /locus_tag="VFW59_01115"
     CDS             complement(5306..5722)
                     /locus_tag="VFW59_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5362846.1"
                     /translation="MKARIKWVEQVSFLGETESGHAVLMDGAPAAGGRNLGPRPMEML
                     LLGAGGCTSFDVIAILKKSRQAVTDCYVELEAERAETDPKVFTKIHMHFVVTGKDIKP
                     EAVEKAIKLSAEKYCSASIMLGATAAMTHDFEVIQE"
     gene            complement(5740..6495)
                     /locus_tag="VFW59_01120"
     CDS             complement(5740..6495)
                     /locus_tag="VFW59_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX5362847.1"
                     /translation="MSWFITNTLAALLLPPLNLLLLLALGLYLLYRRRKFAWPLIAAS
                     FALLWLAATPWVAEGGLHLLEAHTTPLDEQGADAIVILGGGTYFNAPEYGGQDTVNEY
                     ALLRLRYGAKLQRTTGKPILVTGGKPLGNTLSEAQQMKAVLEQDFRVPVRWTENASDN
                     TFENARNSYQMLQQQNIRKIYLVTHAWHMPRAAGVFRRAGFEVVEAPMAYTTRYRIDL
                     LAFLPRAEALNDSRIFMHELIGLLWYRVKSALS"
     gene            6568..>6878
                     /locus_tag="VFW59_01125"
     CDS             6568..>6878
                     /locus_tag="VFW59_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362848.1"
                     /translation="MSASCDDGGAMGGSVTGLEMRHRPSGENARFMGDGLPFDEYLRR
                     TTAMLRRVHAGRADPDKVVAGNAPFELHPVGEPQSGKPYRRGVLLTHGLSDSPYFMRH
                     LA"
BASE COUNT         1253 a         1926 c         2190 g         1509 t
ORIGIN      
        1 cacccttctc gtaggcgatg ccctgtgcct tcgcttgtgt ctcggtcaga cccatccatg
       61 cgatctcggg gtcggtgtaa gcgaccgacg ggatggtcag cggctcgaag aatgccttgt
      121 gtccggcgat gttctccgcc gcgaccttgc cctcatgcac ggccttgtgc gccagcatcg
      181 ggtcgccgac gatgtcgccg atcgcgaaga tgtgcggcac gttggtgcgc atctgtttgt
      241 cgacagggat gaagccgcgc tcgttgacga tcaggcctgc cgcttcggca ttgatctcgc
      301 gtccgttcgg acgacggccc acggccatca gcacgcggtc gtagagttgc ggttcggctg
      361 gtgcctgctc gccttcgaag gtgaccttga ggccgctctt ctgagcttcg atcttggtga
      421 ccttggtctt cagcatgatg gcttcgtaac gcttggcgat acgggtgtgc agcggcttga
      481 ccatgtcctt gtcggcaccg ggcatcaact gatccatcag ctcgaccacg gaaatcttcg
      541 ctcccagcgc gtcgtatacc gtcgccatct ccaggccgat gatgccgccg ccgatgacca
      601 gcatgcgctt gggcacatct ttcagcgcga gtgcgccggt cgagtcgatg atgcgctcat
      661 cgtcgtaagg aaagccgggg atgcgtgcga cgctggagcc cgccgcgacg atggcgttgt
      721 cgaaggtgac gatcttctcg ccttcctcgg tctgcaccgc gatagagttc ggcgaggtga
      781 atttcgccgt gccgcgcacc acctgcacct tgcgttgttt ggcgaggcct gcgagtccgc
      841 cggtgagctt ggtgatgacg ctttctttcc agctgcggat cgcgtcgatg tcgatcttcg
      901 gcttggcgaa ggtcacgccg tgatgcgaaa cttcctctgc ttcgctgatg accttggcga
      961 catgcagcag cgccttggag gggatgcagc cgacgttgag acacacgccg cccaaggagg
     1021 cgtgcttctc gatcagcacg acttgcttgc cgaggtcggc ggcgcggaat gcggcggtgt
     1081 atccgccggg gccggcgccg agcacgacga cttctgcgtg gatgtcaccc ttcgcgacct
     1141 tggcagccgg agtcggcgca gcttgcggtg cggcggcttg tggcgctgcg gttggtgcgg
     1201 tctgtggcgc cggtgcggaa gcgggcgccg cgacagcagc gctgctttcc agcatcagga
     1261 tcaatgtgcc ttcggagacc ttgtcgccga ctttgacttt cagttctttc acgacgccgg
     1321 cagtcggtgt ggggacgtcc atcgtcgcct tgtctgtttc gagcgtgatc agcgtctgtt
     1381 ccgcctcgat ctgctcgccg acttttaccg cgatctcgat gatggcggca tccttgaatc
     1441 cgccgatgtc aggaactttg atttctacga tttggctcat gctgttctct tttccttgaa
     1501 tgctgttaaa cgcggatctg gccgctgccc agtacgatgt atttctgcga ggtcagccct
     1561 tccaggccga ccgggccgcg cgcgtggatc ttgtcggtgg agatgccgat ctccgcgccc
     1621 aggccgtatt cgaagccgtc ggcaaagcgt gtcgaggcat tcaccatcac gctggaagaa
     1681 tccacttcgc gcaggaagcg catcgccttg gtgtagttct cggtgacgat gctgtcggtg
     1741 tgctgcgagc tgtaggtgtt gatgtggtct atggcctcgt ccagattcgc caccacgcgc
     1801 acgctcagga tgggcgcgag gtattcggtg cgccagtcat cgatggtggc ggctgtcatc
     1861 tgcgacacga tggcgcgcgc cgcgtcgtcg ccgcgcagtt ccacgccctt gtcgagatag
     1921 atcttgctca actcgggcag cacctgtgcg gcaacttttt cattcaccag caaggtctcc
     1981 atcgcgttgc acacgccgta acgatgagtc ttggcgttgt cggcgatgcg gatcgctttg
     2041 gtcaggtctg cttcgtcgtc gatatagaca tggcagatgc cgtcgaggtg tttgatgacg
     2101 ggtacacgtg cttcatcgga gatgcgcgcg atcaggctct tgccgccgcg tggcacgatc
     2161 acgtccacat attctttcat ggtgatgagt tcgcccacgg cggcgcggtc ggtggtggtc
     2221 accacttgca cggcagtttc gggcaagcct gccgctttga gtcccgcatg tacgcaggcg
     2281 gcgatggctt ggttggaatg gatggcctca gagccgccgc gcaatatgca ggcgttgccc
     2341 gatttgatgc acaggccggc ggcatccgcc gtcacgttcg ggcgcgattc gtagatgatg
     2401 ccgatcacgc ccagcggcac gcgcatccgg ccgacctgga tgccggaggg gcggaagctc
     2461 atgtcgctga tggaaccgat caaatcgggc agtgtggcga tttgccgcaa gccttcggcc
     2521 atgccttgga cggttttttc ggtgagcgtg aggcggtcca ccgaggcggc atccagaccg
     2581 ttggctttcg ctgcggcgac atccttggcg ttctcggcga tcagccttgc gctgtcgttc
     2641 aggatggcct ccgcgatggc ggtcagcgcg cggttcttgg cggcggtgtc ggcctgcgcc
     2701 atcaggcgcg aagcggcgcg ggcctgttgc ccgacagtct tcatgtattg cttgatctct
     2761 tccatggcat tctcagcgtt gcgtatggcg cgcattttac catcccgctt gcgatagaat
     2821 gtgcccctga tacacaagag accatcatgt ccgacattct gaaaaaaata ctcgcggtga
     2881 aagcgcagga agtggccgcc gcaaaacccg tcaagccgct gccgcagttg cgcgcagaag
     2941 ccgagcagat gccgcccgcg cgcgattttg tcggcgcgat acgaaccatg gtcgaagccg
     3001 gtcggtcggc tgtgatcgcc gaaatcaaga aggccagtcc gagcaagggc gtgatccgcg
     3061 aagacttccg tccggcggag atcgcgcaga gctatgcgca gcacggagcg gcctgcctgt
     3121 cggtgctgac cgacgagcag ttctttcagg gcagcgcgga atatctgcag caggcccgtg
     3181 cggcctgtga tttgccggtg ttgcgcaagg atttcatggt ggacgaatat cagatctggc
     3241 aggcgcgggc gatgggggcg gatgcgatcc tgctgatcgc ggcggcgctg acgctgagcc
     3301 agatgaaggc gttcgagacg ctcgcgcaca agctgggcat ggcggtgctg gtggaggtgc
     3361 atgatgaggc ggagctggag accgcgctgc aactgagcac gccgctgatc ggcatcaaca
     3421 accgcaatct gcgcaccttc gaggtcagtc tgcaaaccac gctggatttg ctggtgcata
     3481 tcgaagaatc caaaaagggc cgcgggcgca tcgtcgtcac cgagagcggc atcttcaccg
     3541 cgcaagatgt gaaactgatg cgcgatcatg atgtgcatgg ttttctggtc ggcgaggcat
     3601 tcatgcgcgc cgatgatccc ggtgcagaac tggccaaggt tttttcctga aggagcaaga
     3661 catgctgaat ctggatcagg tgatcgtcga atttgataaa ggcttgcgca cgctgttcag
     3721 tcaggcgcac agcgcgcgtc cccatcccga tgcgaacgtg cccgatgcgc cgatggacga
     3781 ggccgagcgc aagcatgctg cgggcctgat gcgcatcaat cacagcggcg aggtctgtgc
     3841 gcaggccctg tatcaggggc aggcgctgac ggcacgcgac ccggcggtgc agctgaaatt
     3901 acagtatgcg gctcaggaag agaccgagca tctggcctgg acatcaaagc gcgtgcatga
     3961 gctcggcggt catctgagcg tgctcaatcc gctgtggtat gccggttcgc tgacgctggg
     4021 ggcctgtgcg ggtttgctgg gcgacaagtg gaatctgggc ttcctgtcgg agaccgaaca
     4081 tcaggtcggc ggacatctgc aaagccatct ggatgagctg cccgcgcagg atgcgaagag
     4141 ccgtgccgtg gtgcagcaga tgtatctgga cgagacacag cactcggaga tggccaaaga
     4201 gatgggcggc gcagagctgc ccgagccggt gaagctggtg atgcaggcga cctcaaaaat
     4261 actgacgcaa gccgcctacc ggatttaggt ttcgattgaa accatttaac gggcggcgac
     4321 aagctgcccg ttttcgttat tcctccccac cctgtgctgc tgcaacttcc ttggcgagcg
     4381 agtcggcaca cgccagtagt aatttgcgtt ctgcagcttt catttgaccg aataacgaaa
     4441 tcagctcggc taaagcatct tcctcagcat aaaaatatgc ggacggaaca cacagcactt
     4501 ttgccagatt gcatacagtc aaaaagtcgg gcgcatgttt gccgcgttcg tactggttga
     4561 ttcttgatga ggccgaagat gcgtctatgc cggcacgtat tccaagctgc atttgggata
     4621 gtccggataa ctggcgcgcc gttttcatgc ggcgtgaaaa gagagacgca gtgtaagtat
     4681 gcataatgca tattgtattt ttcctacatg gatattgttg cttttttgat tgaccggaca
     4741 ctaaggaatg cttagtattt gcgcctctct gagagagagg tgcaggcagc tagtcgtatg
     4801 cgctcgtcag agcgtttttc tctggggaaa atcatgcagg aattcgcaaa gttttacgac
     4861 agcctccatg tgcttgtttc ggatggcgag gtcaaggctg cgatccggga agaatttttc
     4921 agactagggc tggttgccaa tgtagaacgc tttttcggac ttttcggaaa ggataaacgt
     4981 tatttcctcg tgagattcgc tacaaccgac gatgcgatca ggacggcgaa tcaatacaag
     5041 ctacgctcct tcgggttcta tggtgtgctt atcgagctga ggggcgagca tggttcaccg
     5101 cagtgaaaaa ttcacggtac agttcaggag cgagagagtg tgtgcagtaa ctgtccaggg
     5161 aggcgctgaa taggttctgt tgtcgttgcg ggcgacctac agggtattga cgcacagcgc
     5221 ctaccagatt tgaggtgctg gtaagtggtg agtcgggtgg ggccttgagc accgaaggtg
     5281 cgccccactt tgcactcact actccttatt cttgaatcac ctcaaaatca tgcgtcatcg
     5341 cggcggtcgc gcctagcatg atggatgccg agcagtattt ctccgccgat agcttgatcg
     5401 ccttctcgac cgcttcgggc ttgatgtctt tgcctgttac gacgaaatgc atgtggatct
     5461 tggtgaacac cttggggtcg gtctcggcgc gttccgcttc cagttccaca tagcaatcgg
     5521 tcaccgcctg acggctcttc ttcaggatcg cgatcacatc gaagctggtg cagccgcccg
     5581 cacccagcag cagcatctcc atcggacgcg ggccgagatt gcgtccgcct gcggccggtg
     5641 cgccgtccat caataccgcg tggccgcttt cggtttcacc cagaaacgag acttgttcta
     5701 cccatttgat gcgtgctttc atctgatgct cctgaatcgt tacgagagtg cggactttac
     5761 ccgataccag agcagtccga taagttcatg catgaaaatc ctgctgtcgt tcagagcttc
     5821 ggcgcgcggc agaaaggcga gcaggtctat tcggtagcgc gtggtatagg ccatcggtgc
     5881 ttcgacgact tcgaagcccg cgcggcgaaa caccccggcg gctcgcggca tgtgccaggc
     5941 atgggtcacc agatagatct tgcggatgtt ttgctgttgc agcatctggt aggagttgcg
     6001 cgcattctcg aaggtgttgt cggacgcatt ttcggtccag cgcaccggta cgcggaagtc
     6061 ctgttccagc acggccttca tctgctgcgc ttcggaaagc gtgttgccca ggggcttgcc
     6121 gccggtcacc agtatcggtt tgcctgtcgt gcgctgcagc ttcgcgccgt agcgcagacg
     6181 taacaacgca tattcattca ccgtatcctg cccgccatat tccggcgcgt tgaaataggt
     6241 gcctccgcca aggatcacga tcgcatcggc gccctgttcg tctaatggtg tggtgtgcgc
     6301 ttccagcaga tgcagtccgc cctcggcgac ccagggcgtg gcggcgagcc acagcagagc
     6361 gaacgaggcg gcgatcagcg gccaggcgaa cttgcggcgg cggtacagca gataaaggcc
     6421 gagtgcgagc aacagcagca gattcagcgg cggcaacagc agggcggcga gggtgttggt
     6481 gatgaaccag gacatggtta tgtgagatgc acgaactgcg cgcattatga catgggcagc
     6541 cgtctgtaat gggagatggc gggtagaatg agtgcatcgt gcgacgacgg gggagcgatg
     6601 ggcggcagcg tgacagggct ggagatgcgg catcggccgt ccggcgagaa tgctcgcttc
     6661 atgggcgacg gtttgccgtt cgacgaatat ctgcggcgca cgacggcgat gctgcggcgc
     6721 gtgcatgcag gcagggcaga tccggacaag gtcgtcgccg ggaatgcgcc gttcgagctg
     6781 catcccgtcg gagagccgca aagcggcaag ccttaccggc gcggtgtgct gctgacgcat
     6841 ggactgtccg attctcccta tttcatgcgc catctcgc
//
LOCUS       DASUIV010000035        16926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_379657, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000035 DASUIV010000000
VERSION     DASUIV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 16926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16926
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379657"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..4183
                     /locus_tag="VFW59_01130"
     CDS             29..4183
                     /locus_tag="VFW59_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HEX5362849.1"
                     /translation="MSMHKRLSPLALGLILNTAFAPVRAETAPVSSSVIVQSFRITGS
                     RAFSETELLKLVDHAVGRQLSRDELIKLADSITGFYRRHGFPRAHAIIPPQNFDRGIV
                     RFQVTTAATRTAGERTEPNRADVSLPPNSGSTDPVQILLEHAQWWVARDRADLAQESL
                     DKLFSIAPDHPSALSLQADIAIRHNRLKEAQAALTALRRAQADHPDIHRIDNLLHALV
                     QNKDQLRHARSLVKAGRYEEAVAIFNKLFPDGPPSDDLTLEYWQMVANTPKGWHLAHQ
                     GLIRLVKNNPDNLHYRLALAEHETSRLPIKRRALQIIVEMSKLPGYSQQARNAWRSAM
                     MKLGDTPASLPLLRHYLSAEPNDSAVREKQVLIVQAQARHNRLMADPDYRAGIEGLAL
                     LDKGELNAAEPLLQQALRARPADGDLIGGMGMLRLRQGRHSEARENFTLAARHTRGGS
                     RKWSSLVKVAKFWQLMQEARDSRKAGDDTLAENKLNAALQVKKNEPDALAALAGIQAD
                     RGQFDAATQTYRHALSIDPSNSEALAGLLTLYRRQGKELEAQQAIAQLSPTQRNALGP
                     TLRHMEASMLQEQAERLLANGQEREAIAVLERAVQADTDDPWLRYTLAKLHARHQQPG
                     KGQALFDELLARHPNDPDALYAMALFQSSQGRSEQVLATLASIPAADRSAKASRLWGA
                     SLERIVDAQVQAGHRNEAKRLLRDAENTASSDEEIQLSVAMAWAGIGEYREADRLFDA
                     LRSASPSVRWRLRHADYLAMKASPELHAELNTLTTMTTLSPDEKRELHALRESLALRT
                     ANTQLAAGEPDLAHQTLAPFLQDTPDHIPLLLAEAQAYRQQQKWPDAGADYAHILRLA
                     PDEADARRGLIETQLAAGNRTAALAQLDAWAGSDTPLSSRLQLVDLYLALDEADSAQE
                     LLDSLLKQQPNNPRVLNQAWQMAQHSGRLDEEIVYLQKTLAAKYHEQASVQAVVPAPQ
                     DPEAYRQIGFDELGSPKKIRRDWQEKKLAALIDRRSDWFSTAIDQRNRSGTPGTSLFH
                     STEIPLEYKTPWHSNDEVFFRTDMVRLDAGSVAATNTLFGSQLLCQPLCATNLLPQTT
                     SGMSFTAGYHRDDLRGDIGITPLNFAVTNIVGSLRRDGDIGQLSYSLEASRRPVTSSL
                     LSYAGTRDPRTGQVWGGVVATGGRLGISLDKGEALGFWSSLGLHNLTGRNVLSNQRFQ
                     LMAGGQWRIVNEENRLLSLGLTGMYWQHSRNAGEYTFGHGGYYSPQSYRSLSLPVSFG
                     ERYPRFSYVLRAAVSASQSQTQAADYYPTDPQLQARAVALTPTNFITPVYAGGPGTGT
                     GYSLKGAWEYQVDPKLFLGGLFSIDRSDNYAPNRALFYLRYSLDQAAAQPVYFQPLPI
                     EPSSQF"
     gene            complement(4276..4599)
                     /locus_tag="VFW59_01135"
     CDS             complement(4276..4599)
                     /locus_tag="VFW59_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362850.1"
                     /translation="MKMFILVPVLLLAACANNPQGGAMAGGAVGGATGAAIGYEIGGQ
                     GGAVLGGAIGGATGAAVGSNASQPTQPVESKAQPVYQKRGHDEEKHREHRRKGRDHED
                     DDEED"
     gene            complement(4625..4939)
                     /locus_tag="VFW59_01140"
     CDS             complement(4625..4939)
                     /locus_tag="VFW59_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362851.1"
                     /translation="MKKSLVMALFLTASVNAYAVDGGAVIGGALGGAAGAAVGYNLGG
                     QGGAILGGALGGATGAAVGTSRQAAPVQVAPAQAMEARDDEEGHHDHGRHRGHEKHHN
                     DD"
     gene            5098..5892
                     /gene="map"
                     /locus_tag="VFW59_01145"
     CDS             5098..5892
                     /gene="map"
                     /locus_tag="VFW59_01145"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5362852.1"
                     /translation="MAVSIKTPQEIEKMRIAGRLTSEVLDYITPFVKAGITTNELDKL
                     CHDYIVDMQQAIPAPLNYAPGGHTPYPKSICTSINHQICHGVPSDKVLKNGDIVNLDV
                     TVIKDGYHGDSSRMFLVGEPSIQARRLCETTWLALWRGIRMVKPGAHLGDIGHAIQSY
                     VEPLGYSVVREFCGHGIGSRFHEEPQVLHYGRPKTGIRLEAGMTFTIEPMINAGKKDI
                     KQLGDGWTVVTKDHSLSAQYEHTILVTETGFEVLTVSDGCPPPPAV"
     gene            5864..8419
                     /locus_tag="VFW59_01150"
     CDS             5864..8419
                     /locus_tag="VFW59_01150"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX5362853.1"
                     /translation="MAALPLPQSDGIAALRESLRGERAALEQAFLQHGKAARLLKAHS
                     RLIDGYLRRIWQQLAMPAELALVAVGGYGRGELFPKSDIDLLILLKDEPDETLQQKLQ
                     ELVGMLWDIGLEVGHSIRTIEQCLAESADITVQTNLLEARLLCGDARLFRQLNKAVQQ
                     HLDPRAFFLAKQQEQLKRHARFVDADFNLEPNLKESPGGLRDLQTVSWICRAAGLGTR
                     WSELAHSGLITAAEARQIARHDALLQTLRIRLHYLAKRREDRLLFDFQTALAEQMNIA
                     ASANRRASEHLMQHYYRTRVAVRQFNTILLQNLRDHIFRDTLPQHALNERFRVIGTLL
                     DIRDEQLFEHSPTAIFESFLLMEQHQELTDLSAKTLRALWRSQHRIDAAFRRDPLNKT
                     RFMEILRQPRGILHALRRMNQYGILGRYLPAFGRIVGQMQHDLFHVYTVDEHILMVVR
                     NLRRFALPEYAHEVPLCSKLMGEFARPDVLYVAGLFHDIAKGRGGDHSQLGKKDARAF
                     CKQHGLGNDDSNLVVWLVEQHLTLSATAQKQDLSDPDVIAAFAGNIRNDRYLVALYLL
                     TVADVRGTSPKVWNAWKGKLMEDLFHLARHYMSDGQSADRLGEVRRDSANQLNLYALS
                     PDRYQLLWAQLDDSYFLDHEAQEIAWHTRQLAFKVNSPEPVVKTRLSRAGEGLQVLVY
                     SPDRRSLFARICDFFARMNFTIVEAKIHTTRHGYALNSFQIMEATPGDQAYRNIMTYV
                     EHELTQQIVQAKPLTLSASGRISRQLKHFPIPTQVDIHPDNKGMLVLSLTAGDRPGLL
                     AHIAQILDQRGVRLHRAKINTLGSRAEDVFWISGAVLAQPEKIEALREALLKK"
     gene            complement(8456..12145)
                     /locus_tag="VFW59_01155"
     CDS             complement(8456..12145)
                     /locus_tag="VFW59_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362854.1"
                     /translation="MSNKPAAIRLVDKDNEKFIAETHAIQEARGRIEAETDADLSAQA
                     RARAEAHARAVAQARAHAEMELTIQIEAKSQDEAKLAEIVRIKLEAEKQAAAELQEKV
                     AAELRLQSESQARLRVEQQAHAELNQRLQHEQLARTAAEEKIRAERELALLAEQRHRV
                     DQDAKQYAEAEIQRMQAEIVELLARTEQAYAERQAAEQAQKRNAEEKLALEEQLRKAA
                     VKRTEMEAEELRLARNKIGVEQAELENIGKRVEAEQRAVQAAENRARMEQELARLAQE
                     KEATDSAIARAAEEKARREQETVSLMTSKLKAEQAAAEAADSRARAEALAVSEVEARI
                     AAHRDAEQTALARAESERAAADMSMQRKLVEEQTRQNVEARERAERQAYDAARQRAEA
                     EALARSEAERKLKAEQEATALSQAQAEADRQVQQQLEQANALLRQIEQETQERLNIET
                     KTAQAAAYKLQAEQDMTVLAQQRAEAELQATREAELALQAEARELEAVNQRAAAAQQA
                     QRLAEQNAATEASLLATIAARMEAEQCAIQKSEESLEAEMRARAQADETERLNTQALE
                     AAREREAAAVRARNIAKVRLEEEEKALKIEQEKIRIDALATAAAQQARRLTEQNLASE
                     AQLQATLEAHSAAEQRALDANNERLMIEARALEQAAETERLNMQLLETAREAEQAAVR
                     ADHLLKARLEQEARALEVEQEKIQAEERAIEAFGKQIAAAERAAQSAMKRAENMARMR
                     IEEERKVIEAEQERLRNEGQATVTLRKRREAEEQAAAAVAKRVEVETAFLMQQQQQAE
                     RERELLDQIEQRQAFAAEADLLMQARLEAEQLAAQAEQERVVAEQLATERANAIVESA
                     RRQQEECELRLSQSRERCAADEQRAVAAEQAQQQQEEQAASLAAQRIEMAEQARLLAE
                     QRERTEVVLLEQQQRHLELERELFEQVARRQALEDETGGLVQARLEAERMVLLAEQEK
                     VAAEQAAMERANQLIDAARKQREEIEQAVSQAIELNVAEEKRISLAEQDRLLSEQAML
                     EQLRERTLIAEQAAQCAVRRQEEEQKLLAEQQAKLEDERRVLIEIEARVAAEAQASAA
                     LRERETAEQRASAAAAEKAAALAEESRLARQRAEQDERDVISLRELNVMSLTKSLMSS
                     GERKFSRLTRMGMAAAAASIIGFALWPFSGREADSLQTAVRINPAITATKPEAVAAQD
                     GDGKQEPTAYPAFDGLKMSDRLGQK"
     gene            complement(12312..12950)
                     /locus_tag="VFW59_01160"
     CDS             complement(12312..12950)
                     /locus_tag="VFW59_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5362855.1"
                     /translation="MSFTEDCRNCPRLAGFLDQVRADHPDYHARPVSSFGAAGSRFLI
                     VGLAPGMHGANRTGRPFTGDHAGNLLYGTLHKYGFATRAEPLDASGNANRALSLQGCR
                     ITNAVRCLPPQNKPEPAEVRACNGYLGEELAALPQGAAVMALGAIAHQAVLMSLQLRK
                     SAHVFAHAARHELPGGLTLFDSYHCSRYNTQTRRLTTEMFEAVVADISRHVA"
     gene            complement(12950..15082)
                     /locus_tag="VFW59_01165"
     CDS             complement(12950..15082)
                     /locus_tag="VFW59_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362856.1"
                     /translation="MASSHTWNFFRAGGFDQVQIETGADMLALRELDQKLWAALSCPT
                     RGIEFDSRTLDLIDSDGDAQVRPNEVLEAIAWAGGMLKNADLLVAGTDHLLLSDIDET
                     SEAGRQLLASARHILKSLGKPEASEISLADMEDVEKLVASQEFNGDGVICAEQIADAG
                     LRATVEDIAACVGSSADMSGQTSITQDIAERFFVEATAYEAWYAQGERDAAVLFLGEN
                     TLAAAQAFHAVRDKIADYFMRCRLAAYDMRAAAPLSRAVEDYQRLAANSLSAGSVEVA
                     GFPLATVEAGKPLPLIEGINPAWQAQLETFRVQVVVPLQGHGETLSDGEWGKLCARFA
                     AYDAWQADKPAGCVEGLGIARLREIFNAQRKQQIEALISQDKEVEAEVKAIRSVEKLL
                     RYKRDLFTLVNNFVSFRNFYTGRAKATFQVGTLYLDGRSCELCVRVEDASKHAGFANM
                     SGACLVYCDCVRNGGKEKMSIVAAFTAGDSDFLMVGRNGIFYDRKGRDWNANIVHIVD
                     HPISIRQAFWSPYKKLAKTISEQLQKFAASKAGAVQGKMVNAAVASGKGIAESSPAAT
                     PPQPFDVGKFAGIFAAMGLAIGAIGGILASIVSGVLGLKFWQIPLALIGLMLLVSGPS
                     MVLAWFKLKKRNLGPILDASGWAINARAYINIPFGTSLTALPLLPKGANRSLVDPYAE
                     KKAVWPYYLLVAAALGALFGLWYTGFFG"
     gene            complement(15168..16019)
                     /locus_tag="VFW59_01170"
     CDS             complement(15168..16019)
                     /locus_tag="VFW59_01170"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX5362857.1"
                     /translation="MLSYRHAFHAGNHADVLKHFVEMQLLQYLGQKDKPFWYIDTHAG
                     AGCYALDSGYATQNAEYESGIARLWQRDDLPAPLAEYVALVKRINPDGQMSLYPGSPL
                     VAQQLLRGQDKMRLFELHPTDSEILQENFAGHGSDVLMQAADGFGALKALLPPPPRRA
                     LVLIDPPYEDKQDYQRVVSALREGLKRFANGVYAVWYPQLQRAEARELPEELKRLPVK
                     SWLHVALTVQTPSEDGFGMYGSGMFVLNPPWTLHGVLQEVMPYLVRQLGQDEGATYTL
                     EQRENLP"
     gene            complement(16127..>16926)
                     /locus_tag="VFW59_01175"
     CDS             complement(16127..>16926)
                     /locus_tag="VFW59_01175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX5362858.1"
                     /translation="LMLKITVADTLAQFVDYGFSNIATLGTNLAWVTIYAFAIQILCD
                     FWGYSTIALGSAYMLGMRLPINFNSPYSSTSLTEFWRRWHITLSSWLRDYLYIPLGGN
                     RNNKTQRNLIVTMTLAGLWHGASWNFVLWGLLNGLWLSAERLLRIGKDKISSEGFFLG
                     LAKKLLVFHGVCLLWVFFRAANLTDAMLYLKRLLMPPYDFSSSVPSTLSVIIILFVLF
                     QKRVGQYIVDERFLTLTLTRQLAMTAVITLLLLAYSQARLDFIYFIF"
BASE COUNT         3307 a         5700 c         4684 g         3235 t
ORIGIN      
        1 ctaagcaaat ggccaacggt tagccgcgat gagcatgcac aaaagactct cgccattggc
       61 gttggggctc atcctgaata cggccttcgc gcccgttcgg gcagagaccg caccggtctc
      121 ctcctccgtc atcgtccaga gcttccgcat cacgggttcg cgggcattct cggaaaccga
      181 gctgctgaag ctggtcgatc acgccgtcgg ccggcaactc agtcgcgacg aactcatcaa
      241 actggctgac tccatcaccg gattttatcg tcggcacggc ttcccgcgtg cccatgccat
      301 cattccgcca cagaattttg acaggggcat cgtacgattt caggtgacga ccgctgcgac
      361 ccggacggca ggcgagcgca ccgaaccgaa ccgagccgat gtatcgctgc cccccaattc
      421 cggcagcacc gatccggtcc agatattgct ggaacacgcg caatggtggg tcgccagaga
      481 tcgtgccgac cttgcgcagg agtcgctgga caaactgttc agcatcgccc cggatcatcc
      541 ctccgcactg tccttgcagg cggatatcgc aatccggcac aaccggctga aagaagcaca
      601 agcggccctg actgccctgc gacgcgcgca agcagatcac cccgacattc atcgcatcga
      661 caacctgctg cacgcactcg tccagaacaa ggatcaattg cgccacgcac gctcgctggt
      721 caaggcgggc cgctatgaag aagccgtcgc cattttcaac aaactgttcc cggatgggcc
      781 gcccagcgac gacctgacgc tggaatactg gcagatggtt gcgaatacgc ccaagggctg
      841 gcatctcgcc catcagggac tcatccggct ggtcaagaac aatccggaca atctgcacta
      901 ccggctggca ttggccgaac acgaaacttc acgcctgccc atcaagcgcc gggcgctgca
      961 gatcatcgtc gagatgagca aactgcccgg ctattcgcaa caggcacgca acgcgtggcg
     1021 cagcgcgatg atgaaacttg gcgacacccc cgccagcctc cccctgctgc gccactatct
     1081 gagcgccgaa ccgaacgact ctgcggtaag agaaaaacag gttctgatcg ttcaggcgca
     1141 agcgaggcac aaccggctga tggccgatcc cgattaccgc gccgggatcg agggcctcgc
     1201 cctgcttgac aagggtgaac tgaacgctgc cgagccgcta ctgcaacagg cattacgtgc
     1261 acgccccgca gacggcgact tgatcggcgg catgggcatg ctgcgtttgc gtcagggacg
     1321 gcacagcgag gcacgggaaa acttcactct ggcggcgcgc cataccagag gcggcagccg
     1381 gaagtggtcg tcactggtca aggtcgccaa attctggcaa ctgatgcaag aggccagaga
     1441 ttcgcgcaag gcaggagacg acacactggc cgagaacaag ctgaatgccg cgctgcaagt
     1501 aaagaaaaat gaacccgatg ccctggcggc tctcgccggc attcaggctg accggggtca
     1561 atttgatgca gccacccaga cctaccggca cgcgctgtcc atcgatccgt cgaatagcga
     1621 agcactggca ggattgctga ctctttaccg ccgacagggc aaggaacttg aggcacaaca
     1681 ggccatcgcg caactgtcac ccacccagcg caacgcgctg ggcccgacgc tcagacacat
     1741 ggaagcgagc atgctgcaag aacaggccga gcgcctgctg gcaaacggac aggaacgcga
     1801 agccatcgca gttctcgaac gagccgtaca ggcggatacc gacgacccgt ggctgcgcta
     1861 cacgctggcc aaactgcatg cccgtcatca gcagccgggc aaggggcagg ccctgttcga
     1921 cgaactgctc gcccgccatc cgaacgaccc cgatgcgctg tacgccatgg cgctattcca
     1981 atccagtcag ggccgttccg aacaggtatt ggccaccctg gcaagtattc cggcggcgga
     2041 tcgttccgcc aaagcctcgc gtttatgggg cgccagcctg gaacggatcg tggatgcaca
     2101 ggtgcaggcc gggcacagga acgaggctaa gcggctgttg cgggatgccg agaacaccgc
     2161 ctccagcgat gaggaaattc agctgtccgt cgcgatggct tgggccggta tcggcgagta
     2221 tcgggaagcc gaccgtttgt tcgatgcgct gcgaagcgcg tctccttccg ttcgctggcg
     2281 tttgcgccat gccgactatc tcgccatgaa ggcatccccg gagctgcacg ccgagctgaa
     2341 caccctcacc accatgacga ccctgtcgcc ggacgagaag cgcgaactgc acgctttacg
     2401 tgaatcgctg gccctgcgca cggccaacac gcagcttgct gcgggcgaac ccgacttggc
     2461 acaccagaca ctcgcgccct tcctgcaaga cacgcccgac cacatccccc tgctgctcgc
     2521 cgaggcccag gcctaccgcc aacagcagaa atggccggac gccggtgccg actacgcgca
     2581 tatattacgg ctggcgccgg acgaagcgga tgccagacgc gggctgatcg agacccaact
     2641 cgccgccgga aaccgcaccg ccgcactcgc gcaactggac gcatgggctg gcagcgacac
     2701 cccgctgtcc agccgtttgc aattggtcga tctctacctt gcgctggacg aggcggatag
     2761 cgcacaggag ctgctcgaca gcctgctcaa acaacagccg aacaatccgc gcgtgctcaa
     2821 tcaggcctgg caaatggccc agcacagcgg gcggctggac gaggagatcg tctacttgca
     2881 aaaaacgctc gctgcaaaat atcacgaaca agcgtcggtg caagccgtcg tgcccgcccc
     2941 gcaggatccg gaagcctacc ggcagattgg cttcgatgaa ttggggtcgc cgaaaaagat
     3001 ccggcgcgac tggcaggaaa agaaactggc cgcgctcatc gaccggcgca gcgactggtt
     3061 ttctacggcc atcgatcagc gcaaccgcag cggaacaccc ggtacctcgc tattccattc
     3121 gaccgaaata ccactcgaat acaagacgcc ctggcatagc aacgatgagg tgtttttcag
     3181 gacggatatg gtccgactgg acgccggcag cgtcgcggcg acgaacacgc tttttggcag
     3241 tcagctgctc tgccagccgc tatgcgccac aaacttgctg cctcaaacga ccagcggaat
     3301 gagttttacg gcgggctacc accgcgacga tctgcgcggg gacatcggca tcacaccgct
     3361 taatttcgcg gtcacgaaca tcgtcggcag cctgcgccgg gacggcgaca tcggccagtt
     3421 atcctactcg ctcgaggcct cgcgacgtcc ggtcaccagc agcctgctat cctatgccgg
     3481 aacacgcgac ccgaggacgg ggcaggtctg gggcggcgta gtcgcgaccg gtggacgcct
     3541 cggcatcagc ctcgacaaag gggaggcatt gggattctgg tcatcgctcg gactgcacaa
     3601 cctgaccggg cgcaacgtgt tgagcaacca gcgcttccag ctcatggctg gcggacagtg
     3661 gcgcatcgtc aatgaagaaa accggctgct ctcgctcggc ctgaccggga tgtactggca
     3721 gcacagccgc aatgcgggcg aatacacttt cggtcacggc ggctattaca gtccacaaag
     3781 ctaccgttcg ctgtcgctgc ccgtctcctt cggcgaacgc tacccgcgct tctcctatgt
     3841 gctgcgcgct gccgtctcgg cctcgcaatc gcaaacacag gcggcagact actatccgac
     3901 cgatccccag ttgcaagcca gggctgtcgc actcaccccg accaatttca tcacaccggt
     3961 ctatgcgggg ggaccgggaa caggaacggg ttattcgcta aaaggcgcat gggaatacca
     4021 ggttgatccg aaactctttc tcggcggact attctcgatc gaccgttccg acaattacgc
     4081 gccgaaccgg gcattgttct acctgcgcta ctcactggac caagctgctg ctcagccggt
     4141 ctacttccag ccgttgccca tcgagcctag ctcgcagttc taacgggctt ttgctcgggg
     4201 aagctgcaag tcttcgatcg ccagatgcaa aaaaggcacg tcaccgtgcc ttttttgttt
     4261 atgccgcgaa gcgcatcaat cctcttcgtc gtcgtcttcg tgatcgcgcc cttttctgcg
     4321 atgctcacgg tgcttctctt cgtcatgtcc acgcttctgg taaaccggct gcgccttgga
     4381 ctcgacaggc tgcgtcggct gactggcatt gctgccgacc gcagcacctg tagccccgcc
     4441 gatggcgcca ccgagtaccg cccctccctg ccccccgatc tcatagccga tggcagcgcc
     4501 ggtcgcaccg ccgactgcac ctccggccat cgcaccaccc tggggattgt tggcgcaagc
     4561 cgccaacaac aatactggaa ccagaataaa cattttcatg acagtactcc ttatgaacaa
     4621 gggatcaatc atcgttgtga tgcttctcat gcccccgatg gcggccgtga tcatgatgtc
     4681 cttcctcgtc gtctcgtgcc tccatcgcct gagctggcgc cacctgaacc ggtgcggctt
     4741 gcctgctggt gcccacggcc gctccggtcg caccaccgag cgcaccgccc agaatcgcgc
     4801 cgccctgacc acccagattg taaccaaccg ccgcaccggc tgcgccgccg agggctccac
     4861 cgatgaccgc accgccatcc acagcgtagg cattcacgct ggcagtaagg aacagggcca
     4921 tcaccaaaga ttttttcatc gtactctccg tagggaaatt ttcatgacat atcgcaccag
     4981 tccgtccggt gcgccagcag tgtaattcct aatcgtattt gtcggcaatc cgaatcgcgt
     5041 gatattctcg cctatcgtca aagcaacatc acccttatta aagcgctacc catagacatg
     5101 gctgtttcca tcaagacccc acaagagatc gagaaaatgc gtatcgccgg tcggctgacc
     5161 agcgaagtcc tcgactacat cacccctttc gtcaaggcgg gcattaccac caacgaactg
     5221 gacaagctct gccacgatta catcgtcgac atgcagcagg cgatccctgc cccgctgaac
     5281 tatgcccccg gcggacacac gccctatcct aagtccatct gcacctcgat caatcaccag
     5341 atctgccacg gcgtgccgag cgacaaggtg ctgaagaacg gcgacatcgt caatctggat
     5401 gtgacggtca tcaaggatgg ctaccacggc gacagcagcc gcatgtttct ggtcggcgaa
     5461 ccgtcgatac aggcccggcg cctgtgcgag accacctggc ttgcgctgtg gcgcggcatc
     5521 cgcatggtca agcccggcgc ccatctgggc gacatcggcc acgccattca gagctacgtc
     5581 gaaccgctgg gctacagcgt ggtgcgcgaa ttctgcggac acggtatcgg cagccgcttc
     5641 cacgaggaac cgcaggtgct gcactatggc cgtcccaaga ccggcatcag gctggaggca
     5701 ggcatgacct tcaccatcga accgatgatc aatgccggca agaaggacat caaacaactg
     5761 ggcgacggct ggacggtggt caccaaggat cacagcctgt cggcccaata cgaacacacc
     5821 atcctcgtca ccgaaaccgg cttcgaagta ctgaccgttt ccgatggctg ccctccccct
     5881 cccgcagtct gatggcatag ccgcgctgcg cgagtcgctg cgcggcgaac gtgcggcgct
     5941 cgaacaggca ttcctgcaac acgggaaagc cgcgcgcctg ctcaaggcgc acagcaggct
     6001 gatcgacggc tacctgcgcc gcatctggca acagttagcc atgcctgcag aactcgcgct
     6061 ggtcgcggta ggcggctacg ggcgtggcga actgttcccc aaatccgaca tcgatctgct
     6121 gatcctgctg aaagacgagc cggatgaaac cttgcagcag aagctgcagg aactggtcgg
     6181 catgctatgg gatatcggac tggaggtcgg acacagcatc cgcaccatcg aacaatgcct
     6241 ggccgaatcc gccgacatca cggtacagac caatttgctg gaagcgcgtc tgctgtgcgg
     6301 cgatgcgcgg ctgttccggc aattgaacaa ggcggtgcaa cagcatctgg atccgcgcgc
     6361 atttttcctc gccaagcagc aggaacagct caagcgccac gcacgctttg tcgatgccga
     6421 cttcaacctc gaacccaatc tcaaggagag ccccggcgga ttgcgcgacc tgcaaaccgt
     6481 cagctggatc tgccgcgctg cgggcctagg aacccgctgg agcgaactcg cccattccgg
     6541 actgatcacg gcagccgagg cacggcagat cgcgcgccac gatgcgctgc tccagacgct
     6601 gcgcatccgc ctgcactatc ttgccaaacg tcgggaggac cgcctgctgt tcgacttcca
     6661 gactgcgctg gccgaacaga tgaatatcgc cgcatcggct aaccgccgcg ccagcgaaca
     6721 cctgatgcaa cactattacc gcacccgggt cgcggtgcgc cagttcaaca ccatcctgct
     6781 gcaaaacctg cgcgaccata tcttccgcga cacgctgccg caacatgcgc tgaatgaacg
     6841 cttccgcgtc atcggcacgc tgctggatat ccgcgacgag caactgttcg aacatagccc
     6901 caccgcgatc ttcgaatcgt tcctgctgat ggaacagcac caggagctga ccgatctgag
     6961 cgccaagacg ctgcgtgcct tatggcgctc gcagcaccgg atagacgccg ccttccgccg
     7021 cgacccgctg aacaaaacac gcttcatgga aatactgcgc cagccgcgcg gcatcctgca
     7081 cgcgctgcgg cgcatgaacc agtacggcat tctgggccgc tacctgcccg ccttcgggcg
     7141 catcgtcggc cagatgcagc acgacctgtt ccacgtctat accgtggacg aacatatcct
     7201 gatggtggtg cgcaacctgc gccgcttcgc gctgccggaa tacgcgcatg aagttccgct
     7261 gtgcagcaaa ctgatgggcg aattcgctcg cccggatgtg ctctacgtcg ccggcctatt
     7321 ccacgacatc gccaagggac gcggcggtga ccattcgcag ctgggcaaga aagatgcgcg
     7381 agcattctgc aaacaacatg gcctcggcaa cgacgatagc aatctggtgg tctggctggt
     7441 cgaacagcat ctcaccttgt cggctaccgc ccagaagcag gatctgtcag accccgacgt
     7501 gatcgccgcc ttcgccggca acattcgcaa tgaccgctat ctggtcgcac tctacctgct
     7561 gaccgtggcc gatgtgcgcg gcaccagtcc caaggtgtgg aatgcctgga agggcaagct
     7621 gatggaggat ctgttccatc tcgcacggca ctacatgagc gacggccagt ccgccgaccg
     7681 gcttggcgaa gtgcgccgcg attcggccaa ccagttgaat ctctatgccc tctccccgga
     7741 ccgttatcag ctgctgtggg cgcaactcga tgacagctat tttctggatc acgaggcaca
     7801 ggagatcgcc tggcatacgc ggcaactggc gttcaaggtg aacagcccgg aacccgtggt
     7861 caagacccgg ctgagccgcg ccggagaagg actgcaggtg ctggtttaca gcccggaccg
     7921 gcgcagcctg tttgcccgca tctgcgattt ctttgcacgc atgaacttca ccatcgtcga
     7981 ggccaagatc cacaccacac gccatggtta cgcgctgaac agtttccaga tcatggaagc
     8041 cacacccggc gatcaggcct accgcaacat catgacctat gtcgagcacg agctgacgca
     8101 acagatcgtc caggccaaac cgctgaccct ctccgccagc gggcgcatca gtcgccagct
     8161 caagcacttc cccattccga cccaggtcga catccatccc gataacaagg gcatgctggt
     8221 gctatcgctg acggcagggg ataggcccgg actgctggcg cacatcgcac agatactcga
     8281 tcagcgcggc gtgcgcctgc accgggccaa gatcaacacc ttggggagtc gtgcggaaga
     8341 cgtgttctgg atcagcgggg cggtactcgc acagccggag aaaatcgaag cgttacgcga
     8401 agccttgctg aagaaataag gggcacttca atctaggcaa gggcatcgcg cgcccttact
     8461 tctgaccgag ccggtcactc atcttcaagc catcgaaagc cggataggct gtcggttcct
     8521 gcttcccgtc gccatcctga gccgctaccg cttccggctt ggtcgccgtg atggccggat
     8581 ttattcttac ggcagtttgc aggctatcgg cttcacgccc ggagaatggc cataacgcaa
     8641 aaccgatgat ggaagcagcg gccgccgcca tccccatcct cgtcaagcgc gaaaatttcc
     8701 gctcacccga actcatcagc gattttgtca gtgacatcac attcaactcg cgcaggctga
     8761 tgacatcccg ctcgtcctgc tcggcacgtt gtcgtgcgag cctgctttcc tcggcgagcg
     8821 cagccgcctt ctccgctgcc gcagcgctcg cgcgttgctc ggcggtttcg cgttcccgca
     8881 acgccgcact ggcttgtgct tcggctgcca ctctggcttc gatctcgatc aggacccgcc
     8941 gctcgtcttc cagcttggcc tgctgttccg ccagcagctt ctgctcttcc tcctggcggc
     9001 gcaccgcaca ctgcgccgcc tgttccgcga tcagcgtccg ttcgcgcagc tgttcgagca
     9061 tggcttgctc ggacaacaag cgatcctgct cagccagaga tatccgtttt tcttcggcga
     9121 cattcagctc gatcgcctgc gagacggcct gttcgatctc ttcccgctgc tttctggccg
     9181 catcgatgag ctgattggcc cgctccatcg cagcctgttc agccgccacc ttctcctgct
     9241 cggccaacaa caccatccgc tcggcctcga gacgcgcctg caccagacca ccggtctcat
     9301 cttccagtgc ctgccggcgc gcaacctgct cgaacaattc tcgctccagt tcaagatgac
     9361 gctgctgctg ttccagcaat acgacttcgg tccgttcgcg ctgctcggcg agcaggcgcg
     9421 cctgctcggc catttcgatt cgctgagccg ccagcgatgc ggcctgctct tcctgctgct
     9481 gctgtgcctg ctccgccgcc acggcacgct gctcgtcggc agcgcatcgt tcgcgggact
     9541 gggacaagcg caactcgcat tcttcctgct gtcgtctggc cgactcaacg atggcgttag
     9601 cgcgctccgt ggcgagctgc tccgctacaa ctcgctcctg ctctgcctgt gcggcgagct
     9661 gttcggcttc cagacgagcc tgcatcaaca gatccgcctc tgcggcgaaa gcctgacgct
     9721 gttcgatctg atcgagcaac tcgcgctcac gctccgcctg ctgctgctgt tgcatcagga
     9781 acgctgtctc gacctcaacg cgtttcgcca ccgcagcagc ggcctgctct tccgcctcac
     9841 gacgcttgcg cagcgtgacc gtagcctgcc cttcgttgcg caaacgttcc tgctcggcct
     9901 cgatgacttt acgctcttcc tcgatgcgca tcctggccat attttcggca cgcttcatgg
     9961 cggattgagc ggctcgctct gcggctgcga tctgcttgcc gaaagcctcg atggcccgct
    10021 cctccgcctg gattttctcc tgctcgacct cgagcgcgcg cgcctcctgt tccaatctgg
    10081 ccttgagaag atggtcggca cgaaccgcag cctgttccgc ttcacgcgcg gtctccagca
    10141 actgcatatt caaacgctcg gtctcggcgg cctgctcaag cgcgcgcgcc tcgatcatca
    10201 gacgctcgtt gtttgcgtcg agcgcacgct gttcggctgc gctgtgcgcc tcaagcgtgg
    10261 cctgcaactg cgcttccgag gccagattct gttcggtcaa gcgtctggcc tgctgagcgg
    10321 cagcggtggc aagggcatcg atgcgtatct tttcttgctc gatcttgagc gccttctctt
    10381 cttcttccag acgaaccttg gcgatatttc tggcccgtac cgcagccgcc tcgcgttcgc
    10441 gcgccgcttc cagagcctgc gtattgagtc gctcggtctc atcagcctgc gctctggcgc
    10501 gcatctcggc ttcgagactt tcctcgctct tctggatggc acactgctcg gcctccatgc
    10561 gcgccgctat ggtcgccagc agactcgctt ccgttgcggc attctgttcg gccaaacgtt
    10621 gcgcctgttg cgctgcggcg gcacgctgat tgacggcctc gagctccctc gcctcggcct
    10681 gcaacgcgag ctccgcctcg cgcgtcgcct gcaactcggc ctcggcgcgc tgttgcgcca
    10741 gcaccgtcat atcctgctcg gcctgtaatt tataagcggc ggcctgggcg gtcttggtct
    10801 cgatattcag ccgctcctgg gtctcctgct cgatctgacg caacagggca ttggcctgtt
    10861 ccagctgctg ctgcacctgc cgatcagcct ccgcctgagc ctgcgacaac gctgtcgcct
    10921 cctgctcggc cttgagcttg cgctcggctt cggagcgcgc caacgcctcc gcctcggcac
    10981 gctgcctggc cgcatcataa gcctgccgct ctgcccgctc gcgcgcttcg acattctgtc
    11041 gggtctgctc ctccaccagc ttgcgctgca tgctcatgtc tgccgcggcg cgctccgact
    11101 cggctcgcgc cagcgccgtc tgctcggcat cccgatgtgc cgcgatccgt gcttcgacct
    11161 cgctcaccgc cagcgcctcg gcgcgcgccc tcgagtctgc ggcttcagca gcagcctgct
    11221 ccgccttgag cttcgaagtc atcagcgaga cagtttcctg ctccctgcgc gccttctcct
    11281 ctgcggctcg cgcaatcgca ctgtcggtgg cttctttttc ctgagccagc cgggccagtt
    11341 cctgctccat ccgggcacgg ttctcggcgg cctgaacggc gcgctgctcg gcttcgacac
    11401 gcttgccgat gttctccagc tcagcctgtt caacgcctat cttgttgcgc gccagacgca
    11461 actcctcggc ttccatctcg gtacgcttga cggcagcctt gcgcaactgc tcttccagcg
    11521 ccagcttttc ttcggcgttg cgcttttgcg cctgctcggc agcctgacgc tcggcatagg
    11581 cctgctcggt acgtgccagc agctccacga tctcggcctg catgcgctgt atctcggctt
    11641 cggcatactg cttcgcgtcc tgatcgaccc gatgacgctg ctcggccagc agcgccagtt
    11701 cgcgctccgc acgaatcttt tcttcggcgg cggtacgggc aagctgctca tgctgcaggc
    11761 gctgattcaa ttcagcgtgc gcctgttgct ccacgcgcag ccgcgcctga ctctccgact
    11821 gcaggcgcaa ttcggcagcg actttttcct gtaattccgc agccgcctgc ttctcggctt
    11881 caagcttgat gcgcacgatc tcggcaagct tggcctcgtc ctgagacttc gcctcaatct
    11941 gaatggtcaa ctccatctcg gcatgggcac gcgcctgagc caccgcccgc gcatgggcct
    12001 ccgcgcgcgc gcgggcctga gcggaaagat ccgcatccgt ctcggcctcg atgcgcccac
    12061 gcgcctcctg gatggcgtga gtttcggcga tgaatttttc gttgtccttg tcgactaagc
    12121 ggatagctgc tggcttgttg gacatggcat tgctccctgt ggctgattga tacgtatttt
    12181 tccgctatcg gaaccctgcc aagataacag ccgggacggc gaatcaaacg tggaataagt
    12241 ctataaaacc gggttatttc aacccgccgt cgatcccgga actgaagaac ggttgcggca
    12301 caggcgaatc ctcatgccac atgcctcgat atatcggcga caaccgcctc gaacatctcc
    12361 gtagtcaggc gtctggtctg agtattatag cggctgcagt ggtagctatc gaacagcgtc
    12421 agcccccccg gcagctcatg ccgcgcagcg tgggcaaaga catgcgcact cttgcgcaac
    12481 tgcagtgaca tcagcaccgc ctgatgggca atcgcgccca gtgccatcac cgccgccccc
    12541 tgcggcaagg ccgccaactc ctcgcccaga taaccgttgc aggcacgtac ctcggcaggc
    12601 tccggcttat tctgcggcgg cagacagcgc accgcattgg tgatgcggca tccctgcagc
    12661 gacaatgccc gattggcgtt gcccgaggca tccaagggct cggcgcgcgt ggcgaaaccg
    12721 tatttgtgca gcgtgccgta cagcagatta cccgcatgat cgccggtaaa cggtcgcccg
    12781 gtacggttcg cgccatgcat accgggagcc agtccgacga tcagaaatcg gctgcctgcg
    12841 gcgccgaatg aactcaccgg ccgcgcatga tagtcgggat gatcggcccg aacctgatcc
    12901 agaaaacccg ccagacgcgg gcagttccgg caatcttcgg taaagctcat catccgaaga
    12961 acccggtata ccacagtccg aacaacgcac cgagcgccgc tgcgaccagc aggtaatagg
    13021 gccacaccgc cttcttctcg gcgtagggat cgaccagcga acggttcgcc cccttgggca
    13081 gaaggggcaa tgcggtcagg gaagtgccga atggaatgtt gatgtaggcc cgtgcgttga
    13141 tggcccagcc gctggcatcc agtatcgggc cgaggttgcg cttcttcagc ttgaaccatg
    13201 ccagcaccat ggacggaccc gacaccagca acatcagccc gatcagcgcc aacggaatct
    13261 gccagaactt caatcccagc actccgctta cgatcgaggc cagaatgccg cctatggccc
    13321 cgatggccaa tcccatcgcc gcgaagatac cggcgaactt gcccacgtcg aacggctggg
    13381 gcggcgttgc ggccggtgag gattcggcta tccccttgcc gcttgccact gcggcattga
    13441 ccatcttgcc ttgcacggcc cccgccttgg aggcggcgaa tttctgcaac tgctcgctga
    13501 tcgtcttggc gagcttcttg tagggcgacc agaaagcctg acggatgctg atcggatgat
    13561 ccacaatatg cacgatgttg gcgttccagt cccgtccctt gcgatcatag aaaataccgt
    13621 tgcgacccac catcagaaaa tccgaatctc cggccgtgaa agcggccacg atactcattt
    13681 tttccttgcc gccgttgcgc acacagtcac agtagaccag acaggcgccg ctcatgttgg
    13741 cgaatccggc atgcttgctc gcatcctcga ccctgacgca cagctcgcag ctgcgtccgt
    13801 ccagatacag cgtgccgacc tgaaaagtcg ccttggcacg gccggtatag aagttgcgga
    13861 acgacacgaa attgttgacc agtgtgaaca ggtcgcgctt gtaacgcagc agcttctcca
    13921 ccgagcggat cgccttgacc tccgcttcga cttccttgtc ctgactgatc agcgcctcga
    13981 tctgctgttt gcgctgcgca ttgaatatct cgcggagccg tgcgataccg agcccttcca
    14041 cgcagcccgc cggcttgtcg gcctgccacg catcgtaggc ggcgaagcgc gcgcacaact
    14101 tgccccattc gccatcgctc agcgtctcgc catgcccttg cagcggcacg accacctgta
    14161 cacgaaaggt ctccagttgc gcctgccagg ccggattaat cccctcgatc agcggcaacg
    14221 gcttgcccgc ttccacggtc gccaacggaa atcccgccac ctcgacactg cccgccgaca
    14281 gactgttggc ggcgagtcgc tggtaatctt cgacggcacg gctcaacggt gcggcggcac
    14341 gcatatcgta agcggccaga cggcagcgca tgaaatagtc ggctatcttg tcgcgcaccg
    14401 cgtggaaagc ctgcgccgca gccagcgtgt tctcgccaag gaagagcacc gccgcatcgc
    14461 gctcgccctg cgcataccag gcctcatagg ctgtcgcctc gacaaagaag cgctcggcaa
    14521 tgtcctgcgt gatgctggtc tgcccgctca tatctgcgga ggaaccgaca catgcggcga
    14581 tgtcttcgac cgtcgcgcgc aatccggcat cggcgatctg ctcggcgcag atcacgccgt
    14641 cgccgttgaa ttcctgactg gccaccagtt tttcaacgtc ttccatgtcc gccagcgaga
    14701 tttcgctcgc ctccggcttg cccagactct tcaggatatg ccgcgccgag gccagcaact
    14761 gcctgcccgc ctcgctcgtc tcgtcgatgt cggacaagag cagatgatcg gtgcccgcga
    14821 ccagcaggtc ggcatttttc agcatcccgc cagcccaggc gatggcttcc agcacctcgt
    14881 tgggacgcac ctgcgcatcg ccgtcgctgt cgatcagatc cagtgttctg ctatcaaact
    14941 cgatgccccg cgtcggacaa ctcagcgccg cccacagctt ctgatcgagt tcccgcaacg
    15001 ccagcatgtc cgcgccagtt tcgatctgca cctgatcgaa accgcccgcg cgaaagaaat
    15061 tccatgtatg agacgacgcc atgtggcctc cctgctgcaa tgaattgttg aacgatgcaa
    15121 ttatcccgaa aacggccctt ccacgagaac cattccagaa atatgcccta aggcaggttc
    15181 tccctctgct ccagcgtgta cgtcgcacct tcatcctgcc ccaactggcg caccagataa
    15241 ggcatcacct cctgtagcac gccgtgcagc gtccacggcg gattgagcac gaacatgccg
    15301 ctgccgtaca tgccgaaacc atcctcgctg ggtgtttgca cggtgagggc gacatgcagc
    15361 cagcttttca ccggcagccg cttgagctct tccggcaatt ccctggcctc cgcgcgctgc
    15421 aactgcggat accacaccgc atacaccccg ttggcaaaac gcttcaggcc ttcacgcaag
    15481 gccgatacca cgcgctggta atcctgcttg tcttcatagg gcggatcgat cagcaccagc
    15541 gcccggcgcg gtggcggcgg caacagcgcc ttcagcgcgc cgaagccgtc ggcggcctgc
    15601 atcaacacat ccgaaccatg cccggcgaaa ttttcctgca ggatctcgct gtcagtcgga
    15661 tgcaattcga acagccgcat cttgtcctgc ccgcgcaaca gctgttgcgc gaccagcggc
    15721 gaaccgggat acagcgacat ctgcccatcc gggttgatgc gcttcaccag cgccacatac
    15781 tcggccaaag gtgcaggcag atcgtcgcgc tgccacaacc gcgcgatgcc gctctcgtac
    15841 tcggcattct gcgtcgcata gccgctatcc agcgcataac accccgctcc ggcatgggta
    15901 tcgatatacc agaacggctt gtccttctgg ccaagatatt gcagcagctg catctcgacg
    15961 aaatgcttca gcacatcggc gtgattgccc gcatggaagg cgtgtcggta gctcagcatg
    16021 gcttgcggtt ctcctggttc atcaaacggt gattcatgct atctgccttt ctgctgcggg
    16081 atgctgcact cattgggata cctgttgtga agccacacgc accacatcag aatatgaaat
    16141 agatgaaatc gagtctggcc tgcgagtagg ccagcaataa caacgtgata acggccgtca
    16201 tcgccaactg cctggtcagg gtaagcgtca ggaaacgctc gtccacgata tattgcccta
    16261 cccttttctg gaacagcaca aacaggatga tgatcacgct gagcgtcgag ggaacactgc
    16321 tgctgaagtc gtacggcggc atcaacagac gcttcaggta aagcatcgca tcggtcagat
    16381 tcgccgcacg gaaaaacacc cagagcagac aaacgccgtg aaacaccaac agttttttcg
    16441 ccagaccgag aaagaaccct tccgaactga tcttgtcctt tccgatgcgc aggagtcgct
    16501 cggcagaaag ccaaagcccg ttcaacagtc cccacagcac aaagttccag ctcgcgccat
    16561 gccataggcc cgccagtgtc atggtgacaa tcaggttgcg ctgggttttg ttgttccggt
    16621 taccccccag tggaatataa agataatctc tcagccagga ggacaaggtg atgtgccagc
    16681 gccgccagaa ctcggttaag gaggttgagc tatagggcga gttgaaattg atcgggagtc
    16741 gcattcccag catataggcc gacccgagcg caatggtcga gtacccccaa aaatcgcaga
    16801 gaatctggat agcgaacgca tagatcgtca cccacgccaa attggttccc agcgttgcga
    16861 tattcgaaaa accataatcg acgaactgcg cgagcgtatc ggcgaccgtg atcttcaaca
    16921 tcaggc
//
LOCUS       DASUIV010000036         5956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_751645, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000036 DASUIV010000000
VERSION     DASUIV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751645"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..371
                     /gene="gatC"
                     /locus_tag="VFW59_01180"
     CDS             84..371
                     /gene="gatC"
                     /locus_tag="VFW59_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5362859.1"
                     /translation="MSLTTQDVQKVARLARLAMNEGEIEAARTQLSGIFTLIAEMQAV
                     DTSGIAPMSHAQDVAQRLRADNVTEANQREAFQAIAPQVEGGLYLVPKVIE"
     gene            375..1829
                     /gene="gatA"
                     /locus_tag="VFW59_01185"
     CDS             375..1829
                     /gene="gatA"
                     /locus_tag="VFW59_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5362860.1"
                     /translation="MLESSLQQQGDALRAKKISSVELTQLYLDRIAALNPRLNAYITL
                     NPEMTLAQAREADSRLAGGSTDALTGIPIAQKDIFCAKGWLTTCGSKMLHNFVSPYDA
                     HVIEQFNRAGAVNLGKTNMDEFAMGSSNETSHYGTVKNPWDTSRVPGGSSGGTAAAVA
                     ARLCAAATGTDTGGSIRQPAALCGISGIKPTYGTVSRYGMIAFASSLDQAGPMGRSAE
                     DLALLLNTMTGFDERDSTSLQREKEDYARDLDKPLNGLRIGLPKEFFAEGLGNDVALA
                     VEAAIAEYKKLGAVVVDISLPNTRLSIPVYYVLAPAEASSNLSRFDGVRYGYRAPEYS
                     DLADMYEKSRAQGFGEEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRLIAQDFTEAYKH
                     CDVIMGPTSPSTAFKLGEKGDDPVQMYLSDIYTIAVNLAGLPGMSIPCGFGANDMPVG
                     LQIIGNYFDEARMLNVAHQYQLATDWHSRAPQGL"
     gene            1848..3299
                     /gene="gatB"
                     /locus_tag="VFW59_01190"
     CDS             1848..3299
                     /gene="gatB"
                     /locus_tag="VFW59_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5362861.1"
                     /translation="MTWEIVIGLEVHTQLSTNTKIFSGASTAYGAEPNTQADAVSIAL
                     PGVLPVLNKGAVERAIKFGLATGSHIAQRSVFARKNYFYPDLPKGYQISQFDLPVVGQ
                     GALTIQVEPLSGNAKPYEKTVRITRAHLEEDAGKSLHGDYHGMTGIDLNRAGTPLLEI
                     VSEPDMCSAAEAVAYAKALHTLVRWIGICDGNMQEGSFRCDVNVSVRKPGAELGTRRE
                     IKNLNSFKFMQQAIDYEVQWQIDTIENGGKIQQATILFNPDTGETRAMRSKEDAHDYR
                     YFPDPDLLPLEISPEWIEQVRTTLPELPQTKQARYVNDFGLSAYDASILTASREMADF
                     FETALSAVPKDAKICANWIMGEVNAQLNRDGLDMAQCPISAQQLGGMLARIVDNTISN
                     SGAKEVFRTMWAEGGDADAIIEAKGLKQVSDSGAIEALVDEIIAANAEKVAEYRAGRD
                     KLFGFFVGLAMKASKGKANPAQLNDILKQKLAG"
     gene            complement(3341..3730)
                     /locus_tag="VFW59_01195"
     CDS             complement(3341..3730)
                     /locus_tag="VFW59_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX5362862.1"
                     /translation="MKNRILQCVAVVASLSFSALASAAALSPTVSFKSDVTPIIHDYC
                     LNCHEPGGKGYEKSGLDLRTYDSLMKGTRFGSVVKPGDSFTSILVQVVDGRVHASIKM
                     PYGMAGGLSKANIGVLKRWIDQGAKND"
     gene            complement(3750..4391)
                     /locus_tag="VFW59_01200"
     CDS             complement(3750..4391)
                     /locus_tag="VFW59_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX5362863.1"
                     /translation="MNKFITLLAFCSIATSALASEVKLETIKVASDVKTIERGVDALM
                     TDCHSCHSLKYIKYRDLIGLGIDKKKVDEWRGDLPLDAALMAQMSVDDAMQSFGKAPP
                     DLSLMTKARDGGANYVYSYLVGYYVTPEGVPGNHIFPETKMPDILGMSEAADEAQRTG
                     IKARAQDIVSFLAWAADPHEHERETLGYYVLAYLLVLTTLLYFVKNQVWSKLK"
     gene            complement(4388..5650)
                     /locus_tag="VFW59_01205"
     CDS             complement(4388..5650)
                     /locus_tag="VFW59_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX5362864.1"
                     /translation="MPGRIIKWIDHRFPLTSFVKHELTGYPTPRNLSYWWNFGFLAGF
                     VLMLQVATGIFLAMHYKADVALSFDSIQHIMRDVNYGWLLRYMHSTGASAFFLVIFIH
                     MARTLYYGSYRAPRELLWWTGQGLLLLLMGTAFMGYLLPWGQMSFWGATVITNLFRAT
                     PFIGDQVVVWLRGDYTVGDATLTRFFALHYLFPFLIIGGVIVHLVALHTVKSSNPSGI
                     DLADKDNIPFHPYFTTKDLYGLGVFLILYCVFVFFLPNSLIEPANNIPANPMQTPNHI
                     VPEWYFLPFYAILRSVPNMVGGVVAMGLSVMIFAFMPFLDRSRIPGGAHYRPMFRALF
                     YAFAIDMGVLGYIGYIPPTDQSILIGQVATFIYFTLFLLLPFLAKVEERWLIKRGLPP
                     EVMALMESEALEITKRKLKPRRRKGEVA"
     gene            complement(5660..>5956)
                     /gene="petA"
                     /locus_tag="VFW59_01210"
     CDS             complement(5660..>5956)
                     /gene="petA"
                     /locus_tag="VFW59_01210"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX5362865.1"
                     /translation="VFILHRTPEEIAAAGASNGGSDPQPDSQRVKNPEWLVVIGVCTH
                     MGCVPNKEGPGWTCHCHGSQYDDSGRVVRGPAPKNLEVPPYRFLAENKIVIGKA"
BASE COUNT         1305 a         1851 c         1731 g         1069 t
ORIGIN      
        1 atgatgtatc ccgtagtcaa agaggtgcgc tgataaaccc ggttatgata acctatctat
       61 cttgttccaa taaagctttt gccatgtctt taactaccca ggatgtacaa aaagtcgctc
      121 gcctcgccag attggcgatg aatgagggcg aaatcgaagc cgctcgcacc caattatcgg
      181 gaattttcac acttatcgcg gagatgcagg cggtcgacac ctcgggcatc gctccgatgt
      241 ctcacgctca ggatgtcgcc cagcgactgc gcgcggacaa cgtcaccgaa gccaatcagc
      301 gcgaggcttt ccaggctatc gctccgcagg tcgagggcgg cctttacctc gttccgaaag
      361 taatcgaata gatcatgctt gaatccagtt tgcagcaaca aggcgacgcg ctgcgtgcca
      421 aaaagatttc cagtgtcgag ctgacccagc tctaccttga ccgcatcgcc gcgctgaatc
      481 cgcgactcaa tgcctacatc acgctaaacc cggagatgac actggcacag gcacgagagg
      541 ccgactcccg tctggcaggc ggatcgaccg acgcgctgac cggcatcccc atcgcccaga
      601 aggacatctt ctgcgccaag ggctggctca ccacctgcgg ctcgaagatg ctgcacaact
      661 tcgtttcgcc ttacgacgcc catgtcatcg agcaattcaa ccgcgcgggc gcggtgaatc
      721 tcggcaagac caatatggac gagttcgcga tgggatcaag caacgagacc tcgcattacg
      781 gcacggtgaa gaatccctgg gacacatcgc gcgttcccgg aggcagctcg ggcggcaccg
      841 ccgctgcggt cgcggcaagg ctgtgcgcgg cggcgaccgg caccgacacc ggcggatcga
      901 tccgtcaacc cgccgcgctg tgcggcatct ccggcatcaa accgacctat ggcacggtgt
      961 cgcgctacgg catgatcgcc ttcgcctcca gcctcgatca ggcagggccg atgggacgca
     1021 gcgcggaaga tctggcgctg ctgctcaaca ccatgaccgg attcgacgaa cgcgattcca
     1081 cttcgctgca acgcgaaaaa gaagattacg cgcgcgacct cgacaagccg ctgaatggac
     1141 tgcgcatcgg tctgccgaaa gagttcttcg ccgaggggct gggcaacgat gtggcgctgg
     1201 ccgtggaagc ggccatcgcc gaatacaaga agctgggcgc tgtcgtggtc gacatcagcc
     1261 tgccgaacac ccggctgtcc attccggtct actacgtgct ggctccggca gaagcgtcga
     1321 gcaacctgtc gcgcttcgac ggcgtgcgct acggctatcg cgcgcccgag tacagcgacc
     1381 tcgccgacat gtacgagaag agccgcgctc agggtttcgg cgaagaagtg aagcgccgca
     1441 tcatgatagg cacctatgtg ctgtcgcacg gttactacga cgcctactat ctgcaggcgc
     1501 agaagatccg ccgcctgatc gcgcaggact tcaccgaagc ctacaagcat tgcgacgtca
     1561 tcatggggcc gacctcgccg tccaccgcct tcaaactcgg cgagaaaggc gacgatccgg
     1621 tgcagatgta tctgtccgac atctacacca tcgcggtgaa tctggcgggc ctgcccggca
     1681 tgtccatccc ctgcggcttc ggcgcgaacg acatgccggt cggcctgcag atcatcggca
     1741 actatttcga cgaggcgcgc atgctgaatg tggcgcacca atatcaactg gcgaccgact
     1801 ggcacagccg cgcgccgcaa ggcctgtaag ggccaaaagg gaacaacatg acttgggaaa
     1861 tcgtcatcgg gctggaagta cacacccagc tctcgaccaa cactaaaatc ttctccggcg
     1921 catcgaccgc ctatggcgca gagcccaaca cccaggcgga tgcggtgagc atcgcgctgc
     1981 ccggcgtgct gccggtactg aacaagggcg cggtcgaacg cgcgatcaag ttcggtctcg
     2041 ccaccggctc gcacatcgcg cagcgctcgg tattcgcgcg caagaactac ttttacccag
     2101 acctgccaaa gggctaccag atcagccagt tcgatctgcc ggtcgtcggt cagggcgcgc
     2161 tgaccattca ggtcgagccg ctgtcgggca acgccaagcc ttacgaaaaa accgtgcgca
     2221 tcacccgggc ccatctggaa gaggatgcgg gcaaatcgct gcacggcgac taccacggca
     2281 tgaccggcat cgacctgaac cgcgcgggca caccgctgct ggagatcgta tccgaaccgg
     2341 acatgtgttc cgccgccgaa gccgtcgcct acgccaaggc actgcacaca ctggtgcgct
     2401 ggatcggcat ctgcgacggc aacatgcagg aaggctcgtt ccgctgcgac gtcaacgtgt
     2461 cggtgcgcaa acccggcgcc gagctgggca cgcgccgcga gatcaagaac ctgaactcgt
     2521 tcaagttcat gcagcaggcc atcgactacg aggtgcaatg gcagatcgac accatcgaga
     2581 acggcggcaa gatccagcag gccaccatcc tgttcaaccc ggataccggc gagacgcgcg
     2641 cgatgcgcag caaggaagac gcgcacgact accgctactt ccccgatccc gatctgttgc
     2701 cgctggaaat ctctcccgag tggatagaac aggtgcgcac cacgctgccg gaactgccac
     2761 agaccaaaca ggcccgctat gtgaacgact tcggcctgtc cgcttacgat gccagcatcc
     2821 tgaccgcatc gcgcgagatg gcagacttct tcgagaccgc gctgtccgcc gtaccgaagg
     2881 acgccaagat ctgcgcaaac tggatcatgg gtgaagtgaa tgcccaactg aaccgcgacg
     2941 gactggacat ggcgcagtgc ccgatcagcg cccagcaact cggcggcatg cttgcgcgca
     3001 tcgtggacaa caccatatcc aactccggcg caaaggaagt gttccgcacc atgtgggcgg
     3061 agggcggcga cgcggatgcg atcatcgaag cgaagggctt gaagcaggtc tccgacagcg
     3121 gcgcgatcga agccctggtg gacgagatca tcgccgccaa tgccgagaag gtcgcggaat
     3181 accgagctgg ccgcgacaag ctgttcggct tcttcgtcgg tctggcgatg aaggccagca
     3241 agggcaaggc caatccggcc cagctgaacg acatcctgaa acaaaaactg gccggctagc
     3301 cgaagttaaa agagccgcag gagcggctct tttggcttga tcagtcgttc ttcgcgccct
     3361 gatctatcca tctcttcagc acaccgatgt tcgccttgga tagcccgcca gccatgccgt
     3421 aaggcatctt gatcgaggca tgcacccggc catcgaccac ctgcaccaga atactggtga
     3481 agctgtcgcc aggcttgacc acgctgccga aacgcgtccc cttcatcagg ctgtcgtaag
     3541 tgcgcagatc cagcccgctc ttctcgtagc ccttgccgcc gggctcatga cagttcagac
     3601 agtaatcgtg aatgataggc gtgacatcgc tcttgaagct gaccgtcggc gacaatgccg
     3661 ccgcactggc caaggcagaa aaggataacg atgcgacaac agcgacacat tgcaatattc
     3721 tgtttttcat agcgcgctcc ccgagaaatt catttcaact ttgaccagac ctgattcttg
     3781 acgaagtaga gcagcgtggt cagcaccagc aggtaggcga gcacgtaata acccagcgtc
     3841 tcgcgctcat gctcgtgcgg atcggcggcc caggccagga acgagacgat atcctgcgcc
     3901 cgcgccttga tcccggtgcg ttgcgcctca tcggccgcct cgctcatgcc gaggatatcc
     3961 ggcatcttgg tctcggggaa gatatgattg cccggcacac cttcgggggt gacgtaatag
     4021 cccaccagat aggaatacac atagttcgcg ccgccgtcgc gtgccttcgt catcagactc
     4081 agatccggcg gcgccttgcc gaaagactgc atggcatcgt ccacagacat ctgcgccatc
     4141 aatgcggcat cgagcggcag atcgccgcgc cattcgtcca ccttcttctt gtctatgccc
     4201 aggccgatca gatcgcggta tttgatgtac ttcaggctgt gacaggaatg gcagtcggtc
     4261 atcagcgcat ctacgccgcg ctctatcgtc ttcacatcgg aagcgacctt gatcgtttcc
     4321 agtttgactt ccgaggccag ggccgatgtg gcgatgctgc agaaagccaa cagggtgatg
     4381 aacttgttca tgcgacttct cctttgcggc gacgcggctt gagttttcgc ttcgtgatct
     4441 cgagcgcctc gctttccatc agcgccatca cttccggcgg caatccgcgt ttaatcagcc
     4501 atcgctcttc caccttggcc aggaacggca gcaacaggaa tagcgtgaaa tagataaagg
     4561 tcgcaacctg tccgatgagt atggactggt ctgtcggggg gatatagccg atgtatccca
     4621 gcacccccat gtcgatcgca aaggcgtaga acagcgccct gaacatcgga cggtaatgtg
     4681 cgccgccggg gatgcgcgaa cgatccagga acggcatgaa ggcgaagatc atgaccgaca
     4741 accccatcgc caccacgccg ccgaccatgt tcggcaccga acgcaggatc gcgtagaacg
     4801 gcaggaagta ccactccggc acgatgtggt tcggcgtctg catcgggtta gccgggatat
     4861 tgttggccgg ttcaatcagg ctgttgggca ggaagaacac gaacacgcag tacaggatca
     4921 ggaatacccc cagaccgtac agatccttgg tggtgaaata cggatggaac gggatgttgt
     4981 ccttgtccgc cagatcgatg ccgctgggat tgctgctctt cacggtatgc aaggccacca
     5041 gatgcacgat caccccgccg atgatcagga acgggaacag atagtgcagc gcgaagaagc
     5101 gggtcagtgt ggcatcgccc acggtgtaat cgccacgcag ccagaccacc acctgatcgc
     5161 cgatgaaggg cgtggcgcgg aacaggttgg tgatgaccgt cgccccccag aacgacatct
     5221 gcccccaggg cagcaggtag cccatgaaag ccgtccccat cagcagcaac agcaagccct
     5281 gtccggtcca ccacagcagt tcgcgcggcg cgcggtagga gccgtaatac agcgtgcgcg
     5341 ccatgtggat gaagatgacc aggaagaatg ccgaggcacc ggtcgagtgc atgtaacgca
     5401 gcagccagcc gtagttcaca tcgcgcatga tgtgctggat cgagtcgaac gagagcgcga
     5461 catcggcctt gtaatgcatc gcgagaaaaa tgccggtggc gacctgcagc atcagcacga
     5521 acccggcgag gaagccgaaa ttccaccagt aactgaggtt gcgcggagtg gggtagccgg
     5581 tcagttcatg cttgacgaag ctggtgagcg gaaaacgatg gtctatccat ttaatgatgc
     5641 gtcctggcat gatcttctcc tatgcctttc cgatgacgat tttgttttcc gccagaaagc
     5701 ggtacggagg gacttccaga ttcttcgggg ccgggccgcg cacgacgcga ccgctgtcgt
     5761 cgtattggct gccatgacag tgacaggtcc agcccgggcc ttccttgttc ggcacgcatc
     5821 ccatgtgggt gcagacgccg atgaccacca gccactccgg attcttcact cgctggctgt
     5881 ccggctgcgg atccgatccg ccattggaag cgcctgctgc ggcgatctct tccggcgtac
     5941 ggtgcagaat gaatac
//
LOCUS       DASUIV010000037        12219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1123687, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000037 DASUIV010000000
VERSION     DASUIV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123687"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFW59_01215"
     CDS             <1..622
                     /locus_tag="VFW59_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5362866.1"
                     /translation="SGAGAQNMRELLKQMGEAHRVAQGFLADPTSAILDIDREVAGTL
                     RDESFPTAHFGVPLAGSLIPWIDKDLGNGQSKEEWKGGAETNKILGRESHPTPIDGLC
                     VRIGAMRCHSLALTIKLKKDVPLEEIEALIKGANPWVKFVPNQRELSVRELTPVAVTG
                     TLDVAVGRVRKMELGPEYLSAFVCGDQLLWGAAEPLRRMLRILLAR"
     gene            781..3399
                     /locus_tag="VFW59_01220"
     CDS             781..3399
                     /locus_tag="VFW59_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEX5362867.1"
                     /translation="MRKSLLKLLGFVVALALSTTVSAMGLGGINVQSALGQPLKADIE
                     LVSLNKAEKASLVARLASADAYKGAGLEFPYGNKFKFQVESRADGSSYLKASSAQPVN
                     DPFVSLLVEVTWSSGRLLREYTFLLDPPGYVPEQPAPAEVVVVAPAAPAEAVAAVEPA
                     AAAPAVEPAVSNEQAAEALKKQAETMRAEAPVDQKALAKSTVAVQESKPAAKSLATLD
                     VTVQRGDTLNMIAAQNKPVDVSLERMLVALYRANAQQFDNKNMNRIKTGKILRMPEQD
                     ELHKLSQSEAVREIRAQAADWNAYRQKLAGAASVSTQPQESQQVSAGKISSSVTDKTP
                     VAKDSAKEVLKLSKGEAPGDKAAGAGKSMSAQDKKNAAQEEEIAKAKAMKEEQSRLAL
                     LEQNVKDMQRLAELKSAAAALAQPASAASEVAPVSAVAAASEVVAASAPVAQPKPKVV
                     APKVVAPQPTLVDEILGEPLYQAAGAAVLLALGGLAYMLNRRKKKPAAAFAAGEEEIG
                     DSTGRMAMPQESSPDTGDFTTLASNEASESRHSDDVDPISEADLFLNFGRDAQAEEVL
                     KEALRNTPDDHRIHLKLLGIYANRVDVKSFSAIALLLKDSGDEAAWQQAASMGRKLEP
                     NNPLYGGDAHIEDADSATMQTPAMSVAPEEASSVDFDLGEAGKTMVLSPEDMPEMEAV
                     PEKQPEMLPESTMDFDLTSTSPSMVAPEMDFDVTSTNPSMHALAQEAEESAEAGLPNL
                     DDLIFDVTSTHPAMPALQPEEPEAKASADEDGMEFTLDFPITEKSEAAVQPEAAEMGL
                     SGISLNLDDIGMPEDSGSEGKDERWHEVATKLDLARAYQEMGDQTGAREILEEVMRDG
                     DEAQREAAQSLIDQLI"
     gene            3473..4102
                     /locus_tag="VFW59_01225"
     CDS             3473..4102
                     /locus_tag="VFW59_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HEX5362868.1"
                     /translation="MKLHPATQILAWCLLVVLLQFLTLAPLLFAAGTILMWAVFVSGR
                     KFAQLLRRTRWVMLTLLLIYAYSTPGQALIEPLGLLSPSREGLLDGLSQLSRLLAALA
                     GLAMLLDRLHRMQLISGLYTLFAPLQWLSLPCFKGLRERFAVRLALTLHYAEVTMLRG
                     KRGWQDTLNGLFESHDEATQHMELPLYRFGLADALLLCASSALLWWGIR"
     gene            4099..4887
                     /gene="truA"
                     /locus_tag="VFW59_01230"
     CDS             4099..4887
                     /gene="truA"
                     /locus_tag="VFW59_01230"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX5362869.1"
                     /translation="MRIALGVEYDGSSFSGWQSQPDRPTVQDALQAALSQIAGETIAV
                     TAAGRTDTGVHGLEQVVHFDVQADRPLSAWLRGVNALLPKSVAVCWAHAVPDEFHARF
                     SAHGRSYRYLLLNRAMRPAIQAGKVGWFHAPLDVAAMQAAARHLLGEHDFSAFRAAEC
                     QAKSPVKTLTQLDIRREGDMIVFDVSAGAFLHHMVRNIVGCLVYVGKGKYPPDWLAGV
                     LESRERRLAAPTFSPDGLYLRRIQYEAKWGLPQMENGMILGVSL"
     gene            4884..5522
                     /locus_tag="VFW59_01235"
     CDS             4884..5522
                     /locus_tag="VFW59_01235"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5362870.1"
                     /translation="MTRAKICGITNEEDALAVAASGADAIGFVFYPRSPRYVAIAKAA
                     QLVAALPPFVTTVGLFVDADEAFVREVLASVPLDVLQFHGDESPDYCVQFGRPYLKAI
                     RVKAGVDLIQCAAHFCTAQALLLDAHVQGVPGGTGTTFDWGLIPQHLPLPVILSGGLD
                     VENVASAIRQVRPYAVDVSSGVEAGKGINTQKGIKDAAKIAAFMHEVNKSNV"
     gene            5515..6711
                     /gene="trpB"
                     /locus_tag="VFW59_01240"
     CDS             5515..6711
                     /gene="trpB"
                     /locus_tag="VFW59_01240"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5362871.1"
                     /translation="MYNYPDSTGHFGQFGGIYMAETLIPAVEELRTQYLRFRDDPEFK
                     VEFAYELKHYVGRPSPVYHAKRLSESLGGAQIYLKREDLNHTGAHKINNAIGQALLAK
                     RMGKKRVIAETGAGMHGVATATVAARFGMECVVYMGAEDIQRQAPNVFRMKLLGATVV
                     PVESGSRTLKDACNEAIRDWVTNVESTFYIFGTAAGPHPYPMMVRDFQCVIGNEAKVQ
                     MPEMIGRQPDAVIACVGGGSNAIGLFHPYIEEKDVQIIGVEAGGHGIASGQHAAPLTA
                     NAKVGVLHGNKVYLIQDENGQIIETHSISAGLDYPGVGPEHCLLKDIGRAQYVAIDDD
                     EAIAAFDALCRFEGIIPALESSHALAHAMKIAPSMSKDKVLLVNLSGRGDKDMNTVAK
                     LKGITL"
     gene            6708..7508
                     /gene="trpA"
                     /locus_tag="VFW59_01245"
     CDS             6708..7508
                     /gene="trpA"
                     /locus_tag="VFW59_01245"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5362872.1"
                     /translation="MNRIDTAFGKLKQQQRRALIPFITAGDPSPQLTVALMHGLVAAG
                     ADVIELGVPFSDPMADGPVIQRASERALKQGMSLRGVLAMVAEFRQQDAATPVVLMGY
                     GNPIEAMGWEVFARRCEEVGVDGVLTVDFPPEESHEAFEHLQRHHIAPIFLLAPTTNE
                     ARIEQVAKLARGYVYYVSLKGVTGAGNLDLDAIEQKMPQLRKHIKLPIGVGFGIRDAA
                     TALAVAKLCDGVVVGSRIVQEIENSTEQNVVANVGKLVKELRQAIDQA"
     gene            7545..8426
                     /gene="accD"
                     /locus_tag="VFW59_01250"
     CDS             7545..8426
                     /gene="accD"
                     /locus_tag="VFW59_01250"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX5362873.1"
                     /translation="MSWFQKLLPPKIKRRDGDAKKTVPEGLWSKCPSCQAVLYHSDLE
                     QNQSVCPKCSHHHRITARTRLDLLLDSEGRFEIGAEVLPIDTLKFKDQKKYSDRLTAS
                     KKTTGEDDALVVMQGSIHAVPVVVAAFEFSFMGGSMGSVVGERFVRGVQVALEQKCPF
                     VCFAASGGARMQEGLLSLMQMAKTCAALTQLNEDKLPFISVLTDPTMGGVSASFAFMG
                     DVVIAEPGALIGFAGARVIQQTVRETLPDGFQRAEFLLEHGAVDMIIDRREMKNQLAT
                     LITLLTKQAAVTEIEAA"
     gene            8492..9757
                     /gene="folC"
                     /locus_tag="VFW59_01255"
     CDS             8492..9757
                     /gene="folC"
                     /locus_tag="VFW59_01255"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX5362874.1"
                     /translation="MPKTLADWLSYLESLHPKTIALGLERVAQVKQRLNLQPDFPVIV
                     VGGTNGKGSVCAMLEAVLHAAGYKVGCYTSPHLLHYNERVRIGKQQASDTELCASFER
                     IEQARADTALTYFEFGTLAAMQCFIEHGVDVAILEVGLGGRLDAVNAFDADCAVVTSV
                     DIDHTDYLGESREQIAYEKAGIFRKGRVSICADSDVPPALREHAQQIGAELWCIGKEF
                     GFRSHQGQWDYFGKCGSRTALPHPALRGAFQLHNASAVLAALDALKERLPVSMEAVRR
                     GLVEVQLAGRFQFVPGRPTLILDVAHNPHAARSLAQNLANLPPAKTFAVFGMLKDKDM
                     AGVVQALEPCIDCWLVAGLAVPRGASAAELAQVLKAGGVHGTIQTFENITQALQHACN
                     AAGENDRIAAFGSFYTVAEAMQARELSVI"
     gene            9779..10435
                     /locus_tag="VFW59_01260"
     CDS             9779..10435
                     /locus_tag="VFW59_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5362875.1"
                     /translation="MAKELTDDELNLRRKARRRLIGAIALTLAVVVILPMVLESQPKP
                     SGQDIELRIPAPDKVGEFVSGAVVSEVAVAPVLAASEVAAPTPVAEPVVEPVPVAQEA
                     RVEAAKVQPVVEKAPEPKVTVGKSAAAQSATASAKSADKPASKAGYVAQVGAFSSAAT
                     AKQEVDKLKKWGFSKAYTEKIGDKTRVRVGPYEDRDKADKARQLIEKHGMHPVVVGIK
                     "
     gene            10432..10923
                     /locus_tag="VFW59_01265"
     CDS             10432..10923
                     /locus_tag="VFW59_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX5362876.1"
                     /translation="MTGFDYTLIAILVASLLLGLWRGLVYESLSLAGWPIAFVLSKLF
                     ASRLAPMMPGDATMRMVLAYIVVFVAALLLWSVLVWLVSRLMKAVGLGGLDRLLGGVF
                     GLVRGVLVVLALVWLAGLTSMPEQSFWRNAQTSRAAEDVALWGKVWLPENVSQRIHYR
                     VRS"
     gene            10933..>12219
                     /gene="purF"
                     /locus_tag="VFW59_01270"
     CDS             10933..>12219
                     /gene="purF"
                     /locus_tag="VFW59_01270"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5362877.1"
                     /translation="MCGILGVVSQIPANQLLYDGLQVLQHRGQDAAGIATLEGRTFHL
                     HKGNGMVRDVFRTRNMRALKGNAGIAHVRYPTAGSAVDHNEAQPFYVNSPFGIVLGHN
                     GNLTNTPELQKQLFMDDLRHVNTNSDSEVLLNVLAHELQSKSKGLRLDIPTIFSAVSG
                     VHRRCRGAYAVVAMIAGYGLLAFRDIYGIRPLVIGVNETPEGNEYLVASESVALDTLG
                     FKFLRDIAPGEAVFVDFDGNLHSQQCSDQARLHSCIFEYVYFARPDSVIDGVSVYESR
                     LYMGEYLAEKLKRTWSEVDIDVVIPIPDSSRPSALQLANHLDIPFREGFVKNRYIGRT
                     FIMPGQAMRKKSVRQKLNAIGVEFKGKNVLLVDDSIVRGTTSREIVQMARDAGALKVY
                     FASAAPPVRFPNVYGIDMPSRTELIATGRSDEEICNE"
BASE COUNT         2383 a         3389 c         3999 g         2448 t
ORIGIN      
        1 ctccggcgct ggcgcgcaga atatgcgtga actgcttaag caaatgggcg aggcacatcg
       61 cgtggcgcag ggttttctcg ccgacccgac ttccgcgatt ctcgacatcg atcgcgaagt
      121 agcaggcact ttgcgcgacg agagttttcc gacggcgcat ttcggcgtgc cgctggccgg
      181 ctccctgatt ccgtggatcg acaaggactt gggtaacggc cagagcaagg aagaatggaa
      241 gggcggggcg gagaccaaca agatactcgg ccgcgaaagc catccgacgc cgattgacgg
      301 cttgtgtgtg cgcatcggcg cgatgcgttg tcacagcttg gcgctgacga tcaagctgaa
      361 aaaggacgtg cctctggagg agattgaagc gcttatcaag ggcgctaatc cctgggtcaa
      421 gttcgtgccg aatcagcgtg agttgtctgt tcgtgagctg acgcccgttg ctgtcaccgg
      481 aacgctggat gtggcggtcg gccgtgtgcg caagatggag ttggggccgg aatatctgag
      541 tgcgttcgtt tgcggagatc agttgctgtg gggcgctgcg gagcctttgc ggcgcatgtt
      601 gcgcatcttg ctggcccgat aagcagaccg cagaaattct gattcagctg taactgaatt
      661 gaatgaggtt gtgaaaaata ccttaattaa gaggtattgt gaatttgaat tatcagcttg
      721 tgctgataac tctatgatgt tattttactt gtccagaata aaaaaggtga gggtggtcgc
      781 gtgcgtaaat cactactgaa gctgctcggg tttgttgtcg ctctggcact gtccacaacg
      841 gtctcggcaa tggggctggg cggaataaat gtgcagtctg cattgggaca gccgcttaag
      901 gccgacatcg aattggtgtc gctgaacaag gccgaaaaag ccagtctcgt ggcgcgtttg
      961 gcgtcggcag atgcctataa gggcgcaggg ctggaatttc cctacggtaa taagttcaaa
     1021 tttcaggttg agagccgcgc cgatggtagt tcctacctca aggcaagcag cgcgcagccg
     1081 gtcaatgatc cctttgtaag cttgctggtt gaggtgactt ggtcatccgg caggctgttg
     1141 cgcgaataca ccttcctgct cgaccctccc ggttatgtgc ctgagcagcc tgctcctgcg
     1201 gaagtcgtcg tcgttgcgcc tgcggctccg gcagaggccg tagctgcggt tgaaccggcg
     1261 gctgccgctc ctgcggttga gccagctgtc tcgaatgaac aggctgccga agcgctgaaa
     1321 aagcaagctg aaaccatgcg ggcagaagcg cctgtcgatc agaaggcgct ggccaagtct
     1381 accgttgccg tgcaggagtc caagccggca gccaagagcc ttgctacgct cgatgtcacc
     1441 gttcaacggg gcgatacgct gaacatgatt gccgcgcaaa acaagccggt ggatgtcagt
     1501 ctggagcgca tgttggtcgc actgtatcgt gccaatgccc agcagttcga taacaagaac
     1561 atgaaccgta tcaagaccgg caagattctg cgtatgccgg agcaagacga gttgcacaaa
     1621 ttgtcgcaat ccgaagccgt cagggagatt cgtgctcaag ctgcggactg gaatgcttat
     1681 cgtcagaaat tggcgggcgc agcatcggtc agcacccagc ctcaggagtc ccagcaggtt
     1741 tccgccggca agatttccag ctcggtcaca gacaagacgc ccgttgcgaa agactcggcc
     1801 aaagaagtat tgaagttgtc caagggcgag gcgcccggcg acaaggctgc gggtgcaggc
     1861 aaatccatga gtgcgcagga taagaagaat gccgcgcagg aagaggagat tgccaaggcc
     1921 aaggcgatga aagaagagca gtcccgtttg gcgttgctgg aacagaacgt caaggatatg
     1981 cagcgtctgg ctgagttgaa gtctgctgcc gccgcactgg ctcagccggc tagcgccgct
     2041 tccgaagtgg ctcctgtttc tgcggttgct gccgctagcg aggtcgtggc tgcatctgca
     2101 cccgtagcac agcctaaacc caaggtcgtt gcgcctaagg tggttgcgcc ccagccgact
     2161 ttggtggacg agattcttgg cgagccgctt tatcaggccg ccggtgctgc ggtgttgctg
     2221 gcgctgggcg gcttggctta tatgctgaat cgtcgcaaga agaaaccggc agcggcgttt
     2281 gctgcgggtg aagaagagat cggtgattcg acaggccgga tggctatgcc gcaggagtct
     2341 tctccggaca cgggggattt caccactctg gcgagtaacg aggccagcga gagccggcat
     2401 tcggatgacg tggatcccat cagcgaagcg gatctgttcc tgaacttcgg gcgcgatgcc
     2461 caggcggaag aggtgctgaa ggaagcgttg cgcaacacgc ctgacgatca tcgcatccat
     2521 ctcaagttgc tcggcatcta cgccaatcgc gtggatgtga agtcgttctc ggccatcgcg
     2581 ctgctgttga aggattccgg tgatgaggct gcctggcagc aggccgcctc gatggggcgc
     2641 aagctggaac cgaataatcc gttgtatggt ggcgatgcgc atattgaaga tgcggacagt
     2701 gcgaccatgc agacgccggc gatgagtgtc gcgcctgaag aggcatcgtc ggtggacttc
     2761 gatctgggcg aagctggcaa gaccatggtc ctgtcgcctg aagatatgcc ggagatggaa
     2821 gccgtgccag agaagcagcc ggagatgctg ccagaatcga cgatggactt cgatctgacc
     2881 tcgaccagcc catccatggt cgcacccgag atggacttcg atgtgacctc gaccaacccc
     2941 tccatgcatg cgctggcgca ggaagcagag gagtcggctg aggctggatt gccaaatctg
     3001 gacgacctga tttttgacgt aacctcgact catccagcca tgcctgcact gcagcctgaa
     3061 gagccagaag cgaaggcatc ggctgatgag gacggtatgg agttcacgct ggatttcccg
     3121 ataacggaaa aatcggaagc agcggttcaa cccgaggccg ctgaaatggg gctatctggc
     3181 atcagtctga atctggacga tatcggcatg cctgaagatt ccggttcgga aggtaaggac
     3241 gagcgctggc acgaagttgc gaccaagctc gatctggccc gtgcctatca ggagatgggc
     3301 gatcagactg gcgcacggga aattcttgaa gaggtaatgc gtgatggcga tgaagctcag
     3361 cgtgaagcgg ctcaatctct gatcgatcag cttatctaat catgcgtttc attttgacgg
     3421 cagctcggtg tagggctgcc gttttctttt tgtccggcgc ggagttctct gcgtgaaact
     3481 tcatcccgct actcaaatac tcgcctggtg cctgctggtg gtgctgttgc agttcttgac
     3541 gcttgccccg ttgctgttcg cggcgggcac gatcctgatg tgggccgtat tcgtgtccgg
     3601 acgcaagttc gcgcaattgc tgcgccgaac ccgctgggtc atgctgacac tcttgctgat
     3661 ctacgcctat agcacacccg gtcaggcgct gatcgagccg cttggtttgc tcagcccgag
     3721 tcgtgaaggg ctgctcgatg ggctgtcaca actgagtcgt ctgttggcgg cattggcggg
     3781 gctggcgatg ctgctggacc gtctgcaccg catgcaattg atctctgggc tctatacctt
     3841 gtttgcaccg ttgcagtggc tgtccttgcc gtgtttcaag gggctgcgtg aacgcttcgc
     3901 ggtgcgtctg gcgttgacgc tgcattatgc cgaggtcacg atgctgcgcg gcaagcgcgg
     3961 ctggcaggac acgttgaatg gcttgttcga atcgcatgac gaagcgacgc agcacatgga
     4021 attgccgctg taccgtttcg gtctggctga tgccttgctg ttgtgcgcca gttcggcact
     4081 gttgtggtgg gggatacgat gaggatagcc ctgggggttg aatatgacgg cagttcgttt
     4141 tccggctggc agagtcagcc tgacaggccg acggtgcagg atgcgttgca ggccgcactg
     4201 agccagatcg caggtgaaac catcgccgtc accgccgccg gacgcaccga taccggcgta
     4261 catggactcg agcaagtggt tcatttcgac gttcaggcag atcgcccgct ttcggcctgg
     4321 ctgcgcggtg tgaatgcact gctgccgaag agcgtcgcgg tgtgctgggc gcatgcggtg
     4381 ccggatgagt ttcatgcgcg tttctctgcg catggccgca gctaccgcta cctgttgctg
     4441 aaccgtgcga tgcgtcccgc gatacaggcc ggcaaggtcg gctggttcca tgcgccgctg
     4501 gatgtggcgg cgatgcaggc ggcggcgcgg catctgctgg gcgagcacga cttcagcgcc
     4561 ttccgtgcgg cggagtgtca ggccaagtct cccgtcaaga cgctgacgca gctggatatc
     4621 cgccgggagg gcgacatgat cgtgttcgac gtgagcgcgg gtgccttcct gcaccacatg
     4681 gtgcgcaata tcgtcggctg tctggtctac gtcggcaagg gtaagtatcc gccggactgg
     4741 ctggccgggg tgctggaatc ccgcgagcgc cgcctcgccg caccgacctt ctctcccgat
     4801 ggcttatact tgcgccgcat tcaatacgaa gcaaaatggg gcttgccgca gatggagaac
     4861 ggaatgatat tgggagtgtc gctgtgacgc gcgcgaagat ttgcggtatc accaacgagg
     4921 aagatgccct tgccgtcgcc gccagcggtg cggatgccat cggttttgtg ttctatccgc
     4981 gcagcccgcg ctatgtcgcc atcgcgaagg cggcgcagct ggttgccgca ttgccgccgt
     5041 tcgtcaccac cgtcgggctg ttcgtcgatg cggatgaggc attcgtccgc gaggtgctgg
     5101 cctctgtgcc gctggacgtg ctgcaattcc acggcgacga atcgcccgac tattgcgttc
     5161 aattcggccg cccttatctc aaagcgatcc gcgtcaaagc cggggtggat ttgatacaat
     5221 gcgcggcgca tttttgcacc gcccaggcgt tgttgctgga tgcccatgtg caaggggtgc
     5281 cgggtggaac cggaacgacc ttcgactggg gcctgatccc gcagcatttg ccgttgccgg
     5341 tgatcctgtc cggcgggctg gatgtggaaa atgtcgcgtc ggccatccgg caagtgcgcc
     5401 cctatgcggt ggatgtatcc agcggcgtag aggccggtaa aggaatcaat acccagaagg
     5461 gcatcaagga tgcggcaaaa atcgcggcat tcatgcatga ggtaaacaag agcaatgtat
     5521 aactatcccg atagcaccgg ccactttggc cagttcggcg gcatctacat ggccgaaacg
     5581 ctgatcccgg cagtggaaga gttgcgcacg cagtatctgc gcttccgcga cgatcccgaa
     5641 ttcaaggtcg agtttgccta tgagctgaaa cactacgtcg ggcgtcccag cccggtctac
     5701 catgccaagc gtctgtcgga aagcctgggc ggcgcgcaga tctatctgaa gcgcgaagac
     5761 ctgaaccaca ccggcgcgca caagatcaac aacgccatcg gccaggcctt gctcgccaag
     5821 cgcatgggca agaagcgcgt catcgccgaa accggcgcgg gcatgcacgg tgtggcgacg
     5881 gctaccgtgg cggcgcgctt cggcatggag tgcgtggtgt acatgggcgc ggaagacatc
     5941 cagcgtcagg cgccgaacgt gttccgcatg aagctgctgg gcgcgacggt ggtgccggtg
     6001 gaaagcggtt cgcgcaccct gaaagatgcc tgcaacgaag cgatccgcga ctgggtcacc
     6061 aacgtcgaga gcaccttcta tattttcggc acggcggcag gcccgcatcc ttacccgatg
     6121 atggtgcgcg acttccagtg cgtgatcggc aacgaagcca aagtgcagat gccggaaatg
     6181 atcgggcgtc agccggatgc ggtgatcgcc tgcgtcggcg gcggctccaa cgccatcggc
     6241 ctgttccatc cctatatcga agaaaaagac gtgcagatca tcggcgtgga agcgggcggt
     6301 cacggtattg ccagtgggca gcatgccgcg ccgctgaccg cgaacgccaa ggtcggcgtg
     6361 ctgcacggca acaaggtgta cctgatccag gacgagaacg gccagatcat cgagacccac
     6421 tccatctccg ccgggctgga ttatcccggc gtcggtcccg agcattgcct gctgaaggac
     6481 atcgggcgcg cgcagtatgt cgcgatcgac gacgacgaag ccatcgcagc gttcgatgcg
     6541 ctgtgccgtt tcgagggcat catccccgcg ctggaatcca gccatgcgct ggcgcatgcg
     6601 atgaagatcg caccgtccat gagcaaagac aaagtgctgc tggtgaacct ctccggtcgc
     6661 ggcgacaagg atatgaatac cgtcgcgaag ttgaagggga tcacgctatg aaccgcatcg
     6721 atacggcctt cggtaaactc aaacaacaac agcgccgcgc gctgatccct ttcatcactg
     6781 cgggcgatcc gtcgccgcag ctcaccgtgg cgctgatgca cgggctggtg gcagccgggg
     6841 cggacgtgat cgaattgggc gtgccgttct ccgacccgat ggcggacggt ccggtgatcc
     6901 agcgcgcctc cgagcgcgcg ctcaaacaag gcatgtcgct gcgcggcgtg ctggcgatgg
     6961 tggcggaatt ccgccagcag gatgcggcga ccccggtggt gctgatggga tacggcaatc
     7021 cgatcgaagc gatgggctgg gaagtgtttg cccggcgttg cgaagaggtc ggtgtggacg
     7081 gcgtgctgac ggtggatttt ccgccggaag agagtcatga ggcgttcgaa catctgcagc
     7141 gccatcatat cgcgcccatc ttcctgctgg ccccgacgac caacgaggcg cgtatcgagc
     7201 aagtcgccaa gctggcgcgc ggctatgtgt attacgtttc gctcaagggc gtgaccggag
     7261 cgggcaatct ggatctggac gccatcgaac aaaagatgcc gcaactgcgc aagcacatca
     7321 agctgcctat cggcgtggga ttcggtatac gcgatgctgc gaccgcgttg gcggtggcga
     7381 agctgtgcga cggcgtggtg gtcggcagcc gtatcgtgca ggaaatcgaa aattcgaccg
     7441 aacagaatgt cgtcgccaac gtcggcaagc tggtcaagga attacggcag gcgatcgatc
     7501 aggcgtaaac gcctttttga aaaagaattt gaggagaaga aattatgagc tggttccaga
     7561 aactgttgcc cccgaagatc aagcgccgtg acggcgatgc caagaagacc gtgccggaag
     7621 gcctgtggag caagtgcccg agctgtcagg cggtgctgta ccactccgat ctggagcaga
     7681 atcagagcgt ctgcccgaag tgcagccatc atcaccgcat caccgcgcgc acccgtctcg
     7741 atctgctgct cgacagcgaa ggccgtttcg agatcggcgc agaagtcctg cccatcgata
     7801 cgctcaaatt caaggatcag aaaaaatatt ccgaccgtct gaccgcgtcc aagaagacga
     7861 ccggcgagga cgatgcgctg gtcgtgatgc agggcagcat ccatgccgtg ccggtggtcg
     7921 tcgcggcatt cgagttcagc ttcatgggcg gctcgatggg ctcggtggtc ggcgagcgtt
     7981 tcgtgcgcgg cgtgcaggtt gcgctggagc agaagtgtcc gttcgtctgc ttcgccgcca
     8041 gcggcggtgc gcgcatgcag gaaggcctgt tgtcgctgat gcagatggcc aagacctgtg
     8101 ccgcgctgac gcaattgaat gaagataagc tgccgttcat ctcggtgttg accgacccga
     8161 ccatgggcgg cgtgtccgcc agcttcgcct tcatgggcga cgtggtgatc gccgaacccg
     8221 gcgcgctgat cggcttcgcc ggcgcccgcg tgatccagca gaccgtgcgc gagaccctgc
     8281 cggatggctt ccagcgtgcc gagttcctgc tggaacacgg cgcggtggac atgatcatcg
     8341 accgccgcga gatgaagaac cagctggcga cgctgatcac cttgctgacc aagcaggctg
     8401 cggtgacgga gatcgaggcc gcttgatcta tagcaaagcg taggcagtgc tgcacaccgc
     8461 atagtgcgtg cagcattgtc tgcaaaatta aatgcccaag acattagccg actggctctc
     8521 ctacctcgaa tccctgcacc ccaaaaccat cgcgctcggc ctggagcgcg tggcgcaggt
     8581 caaacaacgt ctcaatctcc aacccgattt tccggtcatc gtcgtcggcg gcaccaacgg
     8641 caagggctcg gtgtgcgcga tgctcgaggc cgtgctgcat gcggcgggtt acaaggtcgg
     8701 ctgctatacc tcgccgcatc tgctgcatta caacgagagg gtacgtatcg gtaagcagca
     8761 ggccagcgat accgagttgt gcgcatcgtt cgagcggatc gaacaggccc gtgccgatac
     8821 tgccttgacc tatttcgagt tcggcacgct ggccgcgatg cagtgcttta tcgagcacgg
     8881 ggtggatgtg gctatcctcg aagtgggctt aggcggcagg ctggatgcgg tgaacgcgtt
     8941 cgatgccgac tgcgcggtgg tgaccagcgt ggacatcgac cacaccgatt atctgggcga
     9001 gtcgcgcgag cagatcgcct acgagaaggc gggcatcttc cgcaaggggc gcgtgtcgat
     9061 ctgcgccgac agcgacgtgc cgccagcgtt gcgggagcac gcgcaacaga ttggcgcgga
     9121 gctgtggtgc atcggcaagg aattcggttt cagatcgcat caggggcaat gggattattt
     9181 cggcaagtgc ggctcgcgca cggcgctgcc gcatccggca ttgcgcggcg cgttccagtt
     9241 gcacaatgcc agcgcggtgc tggcggcgct ggatgcgctg aaagagcgcc tgccggtgag
     9301 catggaggcg gtgcggcgcg gcctggtcga ggtgcagctg gccgggcgtt tccagttcgt
     9361 gccgggcagg ccgacactga ttctggatgt cgcgcataac ccgcatgcgg cgcgttcgct
     9421 ggcgcagaat ctggccaatc tgcctcccgc caagactttt gcggtgttcg gcatgctcaa
     9481 ggacaaggac atggcgggcg tggtgcaggc gctggagccc tgtatcgact gctggctggt
     9541 ggccggactg gccgtgccgc gcggggcgag tgcggcggaa ctggctcagg tgctgaaagc
     9601 cggcggtgtg cacgggacga ttcaaacctt cgagaatatt acgcaagcat tgcagcatgc
     9661 ctgtaacgcg gcaggcgaaa atgatagaat cgcggccttc ggatcgttct ataccgtggc
     9721 ggaagcaatg caagcccgcg agctttccgt tatctaattg agcaagcagg atcaaggcat
     9781 ggccaaagag ctgacagacg acgaattgaa cctgagacgc aaggcacgcc gccgcctgat
     9841 cggcgcgatc gcactgacgc tggcggtggt ggtgatcctg cccatggtgc tggagagtca
     9901 gcccaagccc tccgggcagg atatcgagtt gcgcattcct gcgccggata aggtgggcga
     9961 attcgtgtcg ggtgcggtgg tctccgaggt agcggtcgcg cctgtcctgg ccgcgtccga
    10021 ggtcgcagcc ccgactcctg ttgcagagcc agtcgttgag ccggtgcccg tcgcgcagga
    10081 agctcgagtc gaagccgcca aggtgcagcc tgtcgttgaa aaggctcccg aacctaaagt
    10141 caccgtcggc aaatctgccg ccgctcaatc cgcgaccgct tcggccaaga gtgcggacaa
    10201 gcccgccagt aaggcaggct atgtcgcaca ggtcggcgcg ttctccagcg cggccacggc
    10261 gaaacaggaa gtggacaagc tgaaaaaatg gggtttttcc aaggcttata ccgagaagat
    10321 cggcgacaag acccgtgtgc gtgtcggccc ttacgaagat cgcgacaagg ctgacaaggc
    10381 gcgccagctg atcgagaagc acgggatgca tccggtcgtc gtcgggatca aatgaccggt
    10441 ttcgattaca cgctcatcgc cattctggtg gcgtcgttgt tgctgggatt gtggcgcggg
    10501 ctggtatatg aatcgttgtc gttggccgga tggccgatcg cgtttgtgtt gagcaagctg
    10561 ttcgcatcca ggctcgcgcc gatgatgccg ggcgatgcga ccatgcggat ggtgctggct
    10621 tatatcgtgg tcttcgtcgc tgcgctgttg ttgtggagcg tgctggtgtg gctggtttcc
    10681 cggttgatga aggcggttgg gttgggtggg ctggacaggc tgctgggcgg cgtgttcggc
    10741 ttggtgcggg gtgtgctggt cgtgctggcg ctggtgtggc tggccggatt gaccagcatg
    10801 ccggagcagt cgttctggcg caacgcgcag accagccggg cggcggaaga tgtcgcgttg
    10861 tggggcaagg tctggctgcc ggaaaatgtt tcgcagcgca tacattacag agttcgtagt
    10921 taaggaaaaa tcatgtgtgg cattcttggt gttgtttcgc aaattcccgc taaccagctg
    10981 ttgtatgacg gcctgcaagt attgcagcat cgcggtcagg atgctgccgg catcgccacg
    11041 ctggaggggc ggactttcca tctgcacaag ggcaacggca tggtgcgcga cgtgttccgc
    11101 actcgcaaca tgcgtgcgct caagggcaac gcgggtatcg cgcatgtgcg ctatccgacc
    11161 gccggttctg cggtggatca caacgaggcg cagcccttct atgtgaattc gccgttcggc
    11221 atcgtgctcg gccataacgg caatctgacc aataccccgg aattgcagaa gcagttgttc
    11281 atggacgatc tgcgtcacgt caataccaac tccgattcgg aggtgttgct caacgtgctg
    11341 gcccatgagt tgcaatccaa atccaagggt ctgcgcctcg acatcccgac cattttctct
    11401 gccgtatccg gcgtacatag acgttgccgt ggcgcctatg cggtggtggc gatgatcgca
    11461 ggttacggtc tgctggcttt ccgcgacatc tacggcatcc gtccgctggt gatcggggtg
    11521 aacgagactc ctgaggggaa cgaatatctg gttgcttccg agagcgtcgc gctggatacg
    11581 ctgggcttca agttcctgcg cgatatcgca cccggcgagg cggtgttcgt ggacttcgat
    11641 ggcaatctgc acagccagca gtgcagcgac caggccaggc tgcattcctg catcttcgaa
    11701 tatgtgtatt tcgcgcgtcc cgactcggtg atcgacgggg tgtcggtgta tgagagccgc
    11761 ctgtacatgg gcgagtatct ggccgagaaa ctcaagcgca cctggagcga ggtggatatc
    11821 gacgtggtga tccccatccc ggactccagc cgcccgagcg ccttgcaact ggcgaaccat
    11881 ctggacatcc cgttccgcga aggctttgtg aagaaccgct acatcggtcg caccttcatc
    11941 atgccggggc aggcgatgcg caagaagtcg gtgcgccaga aactcaacgc gatcggcgtc
    12001 gagttcaagg gcaagaacgt gctgctggtg gacgactcca ttgtgcgcgg caccaccagc
    12061 cgcgagatcg tgcagatggc acgcgatgcg ggcgcgctga aggtgtattt cgcttccgcc
    12121 gcaccgccgg tgcgtttccc caatgtatac ggcatcgaca tgccgagccg cacggagttg
    12181 atcgccaccg gtcgttccga cgaagagatc tgtaacgag
//
LOCUS       DASUIV010000038         5018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_659361, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000038 DASUIV010000000
VERSION     DASUIV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659361"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1330
                     /locus_tag="VFW59_01275"
     CDS             245..1330
                     /locus_tag="VFW59_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362878.1"
                     /translation="MPAEWRELLACWVRRGGNSRWETLLKDAGTANLTLADTLREWLL
                     RQGWASVIEERKHGDWWPQHVELLNLPQLRAALGISDKAQDAQRWQTLREELRASCGD
                     NLAPALLALDELPAHRALARHDLIAALQRWQDAQQSGTRRDFALAARDDTKGLSESEW
                     NWLEQTLDLAEFRIERHTPLLLVAAPLTLAMPHGRLELAACPDFAALTPSSVQAASSA
                     SGTVVRWQLVENRTSFERTAKKREADTGVIWLPGFPPTWWRNAVGHLLDLAPAPAHIA
                     CDPDPAGIAIALKAAELWRERGIDWQPWKMAASDLAALRARKLLTEADRLQIAALQQE
                     PALPAPLAELLDWMLEHGEKGEQEGYL"
     gene            1395..2897
                     /gene="lysS"
                     /locus_tag="VFW59_01280"
     CDS             1395..2897
                     /gene="lysS"
                     /locus_tag="VFW59_01280"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5362879.1"
                     /translation="MTTEQTTPQQDDNQIIAERRHKLAEIRKAGVAFPNDFERKNLAA
                     DLHAQFGEKTHDELEAAQIPVAVAGRMMLKRVMGKASFATIQDMNGKMQLFISNGDTG
                     EEAHNAFKHYDLGDILGATGVLFKTKTGELSVRVTQVRLLTKSLRPLPEKFHGLTDQE
                     QKYRQRYLDLITNEDARDVFKKRTRIIQAIRDFFVRHDYLEVETPMMHSIPGGASAKP
                     FETHHNALDLQMFLRIAPELYLKRLVVGGFEKVFEINRNFRNEGLSTRHNPEFTMLEF
                     YEAYRDYKYLMDFTEKLLREVAQKVLGTTVISYQGKELDLGKPFHRLTMVQAIQKYHP
                     QFTDAQLNDREFVIAELQDLKAKYKPEDGIGGLQLSLFEELAETQLFEPTFIIDYPVE
                     VSPLARSSDKNPAITERFELFVVGRELANGFSELNDAEDQAARFDAQVAAKDAGDEEA
                     MYKDNDYVRALEYGLPPTAGQGIGIDRLVMLLTDSASIRDVILFPQMRPE"
     gene            2991..3656
                     /locus_tag="VFW59_01285"
     CDS             2991..3656
                     /locus_tag="VFW59_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5362880.1"
                     /translation="MIDNDTQSRLLQQYPMLRELPAPEQEALLAAANVMQIPAGAVIF
                     DENQPCMGFPLLLSGSIRVIKAAANGRELQLYRIVPGESCILTSSCLLGHTPYQARGI
                     AEQPLEMVLLPASFFHALLNKHEAFRSYVFHLFSDRLTDLMQLVSAVAFQKLDQRLAK
                     LLIDKPSPIHATHQALADELGSAREMVSRLLKGFAEHGWVRLGREQIDIVDAAALKKF
                     SEL"
     gene            3745..3939
                     /locus_tag="VFW59_01290"
     CDS             3745..3939
                     /locus_tag="VFW59_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX5362881.1"
                     /translation="MKLNVGGIDRILRIVVGLALIALAATGTVGMWGWIGVVPLLTGI
                     FKFCPAYTLLGMNTCPMDKK"
     gene            complement(4008..4850)
                     /locus_tag="VFW59_01295"
     CDS             complement(4008..4850)
                     /locus_tag="VFW59_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5362882.1"
                     /translation="MIEFLIILLVPVAIVLWQGIRIVPQGEEWVVERLGRFSSILSPG
                     LSIIIPFIDKVSYKVTTKDIILDVPQQEVITRDNAVILANAIAFIKVSDLQRAVYGIE
                     NFHEAMRNMVQTSLRSIIGGMDLNQALSSRDRIKAELKAAIADEAVDWGLTVKSVEIQ
                     DIKPSPNMQKAMEMQAAAERERVALVTRSEGEKQAIILNAEARLEAARKDAEAQKVAA
                     EASAESMRLIAESVKENNNSATFLLGDRYIQSIQKLSASENSKVVLLPGDIIAAVKNL
                     VAPK"
BASE COUNT         1072 a         1590 c         1442 g          914 t
ORIGIN      
        1 gattgctggc ccctaaaagg ggcctaaccg caatcggtga aagtcaaaag ccaaccccac
       61 tgctggggtg aggcgcactc ccggaaatgt caaacttcgg gaatcacccc ggcatagccg
      121 ggggtttacc atcatcaatt acatgaacac acttgattca attcaaatcg aagcattgga
      181 caagcgcgcc gtctgccata agcggcggcg cacggcgcgg ggtgctccgc aagcattgga
      241 caccttgccc gcagaatggc gcgaactgct ggcatgctgg gtcaggcgcg gcggcaacag
      301 ccgctgggaa acgctgttga aagatgcggg cacggccaac ctgacacttg ccgacacgct
      361 gcgcgaatgg ctgctgcgcc aaggctgggc cagcgtgatc gaagaacgca aacatggcga
      421 ctggtggcca caacacgtag aactgctgaa cctgccgcaa ctgcgcgccg ccctcggcat
      481 cagcgacaaa gcgcaagacg cgcaacgctg gcaaacgctg cgcgaggaac tgcgggcaag
      541 ctgcggcgac aacctcgccc ctgccctgct cgcactggat gaactgcccg cgcatcgcgc
      601 cctcgcccgc catgacctga tcgccgcctt gcagcgctgg caggacgccc aacaaagcgg
      661 cacgcgccgc gactttgcgc tggctgcgcg ggacgacacc aagggcttga gcgagtcgga
      721 atggaactgg ctggaacaga cgctcgacct ggccgaattt cgcatcgaac gccacacgcc
      781 gttgctgctg gtcgcggccc cgctgacgct ggccatgccg cacgggcgac tcgaactggc
      841 cgcttgccca gatttcgccg cgctcacgcc ctcctccgtt caagccgcca gcagcgcatc
      901 cggcacggtc gtccgctggc aactggtgga gaaccgcacc agcttcgagc gcactgccaa
      961 aaaacgcgag gcggacaccg gagtgatctg gctgcccggt ttcccgccaa cttggtggcg
     1021 caacgcggtc ggacacttgc tcgacctggc tcccgccccg gcgcacatcg cttgcgaccc
     1081 agaccctgcg ggcatcgcca tcgcgctcaa ggccgcagag ctgtggcgcg agcgcggcat
     1141 cgattggcaa ccgtggaaaa tggcagccag cgatctcgct gcactgcgtg cgcgcaaact
     1201 gctgaccgag gcagacagac tccagatcgc ggcgctgcaa caggaacccg cgctgcccgc
     1261 cccgctcgcc gaactcctcg actggatgct cgaacatggc gaaaaagggg aacaggaagg
     1321 ctacctgtag cccggaaatg accgcccctg tggtatattc gcgagctgcc aaaaaattct
     1381 tttttgatac catcatgacg actgaacaaa ccactcccca gcaagacgac aaccagatca
     1441 tcgcggaacg ccgccacaaa cttgccgaga tacgcaaagc gggcgtggcc ttccccaacg
     1501 acttcgaacg caagaatctg gcggccgatc tgcatgcgca gttcggcgag aagacgcacg
     1561 acgaactcga agccgcgcag attccggtcg cggtggccgg gcgcatgatg ctgaagcgcg
     1621 tgatgggcaa ggccagcttc gccaccattc aggacatgaa cggcaagatg cagttgttca
     1681 tcagcaatgg cgataccggc gaggaagcgc acaacgcgtt caagcattac gacctcggcg
     1741 acatcctcgg cgcgaccgga gtgctgttca aaaccaagac cggcgagctt tccgtgcgcg
     1801 tcacgcaggt gcgcctgctg accaagtcgc tgcgtccgct gccggaaaaa ttccacggcc
     1861 tgaccgacca ggaacagaaa taccgccagc gttacctcga cctcatcacc aacgaggatg
     1921 cgcgcgatgt gttcaagaag cgcacccgga tcatccaggc gatacgcgac ttcttcgtgc
     1981 gccacgacta tctggaagtg gaaaccccga tgatgcattc catccccggc ggcgcatcgg
     2041 ccaagccttt cgagacgcac cacaacgcgc tggacttgca gatgttcctg cgcatcgcgc
     2101 ccgagctgta cctgaagcgt ctggtcgtgg gcggcttcga gaaggtgttc gagatcaacc
     2161 gcaacttccg caacgagggg ctttccacgc gccacaaccc ggaattcacg atgctggaat
     2221 tctacgaggc gtaccgcgac tacaagtacc tgatggactt caccgaaaag ctgctgcgcg
     2281 aagtcgcgca aaaggtgctg ggcacgaccg tcatcagcta tcagggcaag gagctggatc
     2341 tgggcaagcc gttccaccgt ctgaccatgg tgcaggcgat ccagaaatat catccgcaat
     2401 tcaccgatgc gcagttgaac gaccgcgaat tcgtgatcgc cgaactgcaa gacctgaagg
     2461 ccaagtacaa gccggaagac ggcatcggcg gcctgcaatt gtcgctgttc gaagaactgg
     2521 ccgagacgca attgttcgag ccgaccttca tcatcgacta cccggtcgaa gtctcgccgc
     2581 tggcgcgcag cagcgacaag aacccggcca tcaccgaacg cttcgagctg ttcgtcgtgg
     2641 gccgcgaact ggcgaacggc ttctccgagt tgaacgacgc ggaagaccag gccgcgcgct
     2701 tcgatgcgca agtcgccgcg aaagatgcag gcgatgaaga agccatgtac aaggacaacg
     2761 actacgtgcg cgcgctggaa tacggcctgc cgccgaccgc aggacagggt atcggcatcg
     2821 accgtctggt gatgctgctg accgacagcg ccagcatccg cgacgtgatc ttgttcccgc
     2881 agatgcgccc ggaatagtga actcccctcg cccgcttgcg ggagagaggc tgtgggagag
     2941 ggtaacgcga tgaagcgctt tatcctcaag aactcaactc aatcatcaat atgatcgaca
     3001 acgacaccca atcccgcctg ctccagcagt atccgatgct gcgcgagctt cccgccccgg
     3061 agcaggaagc tttgctggcg gccgcgaatg tgatgcagat tccggctggc gcggtgatat
     3121 tcgacgagaa ccagccgtgc atgggttttc cgctgctgct gtccggcagc atccgcgtca
     3181 tcaaggccgc agcgaacggg cgcgagttgc agttgtaccg catcgttccc ggcgagagct
     3241 gcatcctgac cagcagttgc ctgctcggcc acacgcccta tcaggcgcgc ggcatcgccg
     3301 aacagccgct ggaaatggtg ttgctgcccg ccagtttttt tcatgcgctg ctcaacaagc
     3361 atgaagcgtt tcgcagctac gtgttccacc tgttttccga ccgcctcacc gacctgatgc
     3421 aactggtctc cgccgtcgcc ttccagaagc tcgaccagcg cctcgccaaa ctgctgatcg
     3481 acaaacccag cccgatccac gccacccacc aagccctggc cgacgaactc ggcagcgcgc
     3541 gcgagatggt cagccggttg ctgaaaggct ttgccgaaca cggttgggtg agactgggac
     3601 gcgagcagat cgacatcgtc gatgcggcag ccctcaagaa attctccgag ctctgacgtt
     3661 gctgttgtgt gatttttatc acagacagac aactccgcct cccctattct gagcgctcct
     3721 taaaaccaat ctctggagct gaacatgaaa ctcaacgtag gcggtataga tcgtattttg
     3781 cgcatcgtgg tcggtctcgc tctgatcgcg ctggcagcca caggtacagt cggcatgtgg
     3841 ggctggatag gcgtagtgcc gctgctgacc ggcatcttca agttctgccc ggcttacacg
     3901 ctgctcggca tgaacacctg cccgatggac aagaaataag cgtttagctg tcacccgcaa
     3961 cgcccgatag actgaacaag tcatcgggcg ttgtgctttt gggcgtgcta tttcggagca
     4021 accagattct tgacggcggc gatgatgtcg ccgggcagca gcaccacttt gctgttctcc
     4081 gaagcggaaa gtttctggat ggactggatg taacggtcgc ccagcaggaa ggtcgccgaa
     4141 ttgttgtttt ccttgaccga ttcggcgatc aggcgcatcg actcggcgct cgcttcggcg
     4201 gcaactttct gcgcttcggc atccttgcgg gcggcttcca gacgcgcttc ggcgttcagg
     4261 atgatcgcct gcttctcgcc ttccgagcgc gtcaccagcg cgacgcgttc gcgttcggct
     4321 gcggcctgca tctccatcgc cttctgcatg ttcggcgaag gcttgatgtc ctgaatttcc
     4381 accgacttga cggtcaggcc ccaatcgacc gcctcgtcgg cgatggccgc tttgagttcc
     4441 gccttgatcc ggtcgcgcga cgacagcgcc tggttcagat ccatgccgcc gatgatggag
     4501 cgcaggctgg tctgcaccat gttgcgcatc gcctcgtgga aattctcgat gccgtaaacc
     4561 gcgcgctgca aatcgctgac cttgatgaac gcaatcgcgt tcgccagtat caccgcgtta
     4621 tcgcgggtga tcacctcctg ctgcggcacg tccagaatga tgtccttggt ggtgaccttg
     4681 tagctcacct tgtcgataaa cgggatgatg atgctgaggc cgggactgag aatggagctg
     4741 aaccgcccca gccgctccac cacccactcc tcgccttgcg gcacgatacg tatgccctgc
     4801 cacagcacga tggcgacagg aaccagcagt attatcaaaa attcgatcat gtttttctcc
     4861 tcgataaata cttttaaaaa tcaaaacacc tctgtcgtca ttccggcgaa ggcccactac
     4921 tgtccggaat aaattttgtt gacccgatgg aagtgttgca gtgcatggtt gtatgggata
     4981 gaacccagag cgccaactca acaaccgaga ctgcaaca
//
LOCUS       DASUIV010000039         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_380906, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000039 DASUIV010000000
VERSION     DASUIV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380906"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFW59_01300"
     CDS             <1..520
                     /locus_tag="VFW59_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5362883.1"
                     /translation="VNKWDGLDAHQRDTVKSDIERKLHFLSFAKLHFISALNGSGVDK
                     VLKSVEEAYAAAMIKLPTPKLTRALLTALEKQQPPKGSRFRPKMRYAHQGGSNPPLII
                     VHGSGLDDVPASYQRYLERAFCEMFQLQGTPLKIQFNSSKNPFEGKKRVLTEAELHRA
                     HRARIISRKRWG"
     gene            complement(538..1377)
                     /locus_tag="VFW59_01305"
     CDS             complement(538..1377)
                     /locus_tag="VFW59_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5362884.1"
                     /translation="MIWETAKNTVVLGATLYGALLLALFLLQDRLVYFPNQPGRDYEA
                     TPALLNLPFESVALTSSDGVKLAAWYVPAENARGAVLYAHGNAGNISHRLDAIRQFRD
                     MGLSVFIFDYRGYGQSTGKPSEEGTYRDAEAAWQHLTAQRGIAPAHIVIFGESLGAAV
                     AAHLAARHQPGTLILASAFTSMPELAADLYPFIPARWLTRFHYDTRLHLNDVHAPVLI
                     AHSREDEIIPFRHGKALYDAAHEPRQFLELRGGHNDLFYANTEIFAREVGRFLARHLT
                     PQR"
     gene            1538..2941
                     /gene="leuC"
                     /locus_tag="VFW59_01310"
     CDS             1538..2941
                     /gene="leuC"
                     /locus_tag="VFW59_01310"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5362885.1"
                     /translation="MSAQTLYDKLWNSHVVRQDADGTALIYIDRQLVHEVTSPQAFEG
                     LKLAQRKPWRTASMLATPDHNVPTTNRSGGISDPISRLQVETLDANCAEFGVVEFKMN
                     DLRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVMATQCLV
                     AKKSRAMLVKVEGALGHGVTAKDIALAVIGKIGTAGGTGYAIEFAGSAIRALSMEGRM
                     TLCNMAIEAGARAGMIAADDITIDYVKGRPFAPKADQWDKAVAYWRTLHSDEGAAFDA
                     VVELDAAAIRPQVTWGTSPEMVVSVDGCVPDPASVSDAVKRGDMERALVYMGLSANMP
                     ITDIRIDKVFIGSCTNGRIEDMRAAAAVAKGKKVAANVKLAMVVPGSGLVKAQAEKEG
                     LDKILVAAGFEWRDPGCSMCLAMNDDRLEPGERCASTSNRNFEGRQGQGGRTHLVSPE
                     MAAAAAIAGHFVDVSKL"
     gene            2953..3225
                     /locus_tag="VFW59_01315"
     CDS             2953..3225
                     /locus_tag="VFW59_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362886.1"
                     /translation="MKKISMLLLMLIAVAGNVASAAEEGVVDKAGHGIQKGGEAAGRG
                     IEKGADATGEALKKGGEATVKGVKKAGKWVGKKLQKGGEKLEKASE"
     gene            3225..>3738
                     /gene="leuD"
                     /locus_tag="VFW59_01320"
     CDS             3225..>3738
                     /gene="leuD"
                     /locus_tag="VFW59_01320"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5362887.1"
                     /translation="MRKFSLLDGLVVPLDRANVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDCSKRPVNPDFVLNQPRYQGAQILLARENFGCGSSREHAPWALEDY
                     GFRVIIAPSFADIFFNNCFKNGLLPIKLDADKVDALFKAVEANVGYKLKVDLEQQTIT
                     LPDGTTYQFDV"
BASE COUNT          757 a         1037 c         1257 g          687 t
ORIGIN      
        1 ggtgaacaag tgggacgggc tggatgcgca tcagcgcgat acggtcaaga gtgacatcga
       61 acgcaagctg cacttcctca gcttcgccaa gctgcacttc atctctgcac tgaacggcag
      121 cggcgtggac aaggtgctga agtcggtgga agaggcctat gcggcggcga tgatcaagtt
      181 gccgacgccg aagctgactc gcgcgctgtt gactgcgctt gagaagcagc aaccgcctaa
      241 gggctcgcgc ttccgtccca agatgcgcta tgcccaccag ggtggcagca atccgccgct
      301 catcatcgtg catggcagcg gtctggacga cgtgccggcc agctatcagc gctatctgga
      361 acgcgccttc tgcgagatgt tccagctgca gggcacgccg ctgaagatcc agttcaactc
      421 cagcaagaat ccgttcgagg gcaagaagcg ggttctgacc gaggcggaac tgcaccgcgc
      481 gcaccgcgca cgcatcatca gccgcaagcg ctggggctag acgtcccggg ccagcggtca
      541 gcgttgcggc gtcaagtgcc gcgctaaaaa acgccctacc tcccgcgcga atatttccgt
      601 gttcgcataa aacaaatcgt tgtgtccgcc gcgcagttcc agaaactgac gcggctcatg
      661 cgcggcatcg tagagcgcct tgccgtggcg gaaggggatg atctcgtcct cgcggctgtg
      721 cgcaatcagt acgggcgcat gtacgtcatt caggtgcagt ctggtgtcgt aatggaagcg
      781 ggtgagccag cgtgcgggaa tgaagggata gagatcggcg gccagttccg gcatcgaggt
      841 gaaggcggat gcgagtatca gcgtgcccgg ctgatgtcgt gcagccagat gagccgcgac
      901 cgcagcgccc agcgattcgc cgaatatcac gatgtgcgcg ggggcgattc cccgttgtgc
      961 ggtgagatgt tgccaggcgg cttcggcatc gcgataagtg ccttcttcgc tcggtttccc
     1021 tgtgctctgg ccgtagcctc ggtagtcgaa gatgaacacc gataacccca tgtcacggaa
     1081 ttgccggatg gcatccaggc ggtgcgagat gttgcccgca ttgccgtggg catagagcac
     1141 cgcgccgcgc gcattttctg cgggtacata ccatgcagcc agcttgacgc cgtcgctgga
     1201 tgtcagcgcg acagattcga atggcaggtt gagcagggct ggcgtggcct cgtagtcgcg
     1261 gccaggctgg ttcgggaagt agacgagacg atcctgcagc aggaatagcg ccaacagcag
     1321 cgcgccatac agtgtggcgc cgaggacgac ggtgtttttc gcagtttccc agatcatgcc
     1381 ggtcagaagt cggaagcggg aacagactat cgcataattt ttcccatcgc aggttgccct
     1441 gcagcgatct gccgatttgt ccgctggctg ccaagccagc gcgggttatg gcataatcgc
     1501 gcgctcttga aacgagcatt gggcaagcaa tacgattatg agcgcgcaaa ctctttacga
     1561 caaactctgg aattcccacg tggtgcggca ggatgccgac ggcaccgcgc tgatctacat
     1621 cgaccgtcaa ctggtgcacg aagtgaccag cccgcaggcc ttcgagggct tgaagctggc
     1681 gcagcgcaag ccctggcgca ccgcctcgat gctggcgacg ccggatcaca acgttcctac
     1741 taccaaccgc agcggcggca tcagcgatcc gatctcgcgt ctgcaagtgg agacgctgga
     1801 tgcgaactgt gccgagttcg gcgtggtgga gttcaagatg aacgatctgc gtcagggcat
     1861 cgtgcatgtg atcggccccg agcagggcgc gaccctgccc ggcatgacgg tggtgtgcgg
     1921 cgattcgcat accagcacgc acggcgcatt tgccgcactc gcgcacggca tcggcacctc
     1981 cgaggtcgag catgtgatgg cgacgcagtg cctggtcgcg aagaaatcca gagcaatgct
     2041 ggtcaaggtc gaaggcgcgc tgggtcacgg tgtgaccgcg aaggacatcg cgctggcggt
     2101 gatcggcaag atcggtacgg cgggcggcac cggttatgcc atcgaattcg ccggcagcgc
     2161 gatccgcgcg ttgtcgatgg aaggccgcat gacgctgtgc aacatggcga tcgaggctgg
     2221 cgcgcgcgcg ggcatgatcg cggcggacga tatcaccatc gattacgtca agggtcgccc
     2281 gttcgcgccc aaggccgatc agtgggacaa agccgtggcg tactggcgca ctctgcattc
     2341 ggacgaaggc gccgcattcg atgcggtggt cgagctggat gctgccgcta tccgtccgca
     2401 agtgacttgg ggcacgtcgc cggagatggt ggtatctgtc gacggttgtg ttcccgatcc
     2461 cgcctctgtc tccgacgcgg tgaagcgcgg cgacatggag cgcgcgctgg tttacatggg
     2521 gctgtcggcg aacatgccga tcaccgatat caggatcgac aaggtattca tcggttcctg
     2581 caccaacggg cgcatcgaag acatgcgcgc ggcggcggca gtggccaagg gcaagaaggt
     2641 cgctgcgaat gtgaagctgg cgatggtcgt gcccggttcc ggtctggtga aggcgcaggc
     2701 ggaaaaagaa ggtctggaca agattctggt cgcggccggt tttgagtggc gcgatcccgg
     2761 ctgttcgatg tgtctggcga tgaacgatga tcggctggag ccgggcgaac gctgtgcctc
     2821 gacctctaat cgaaacttcg aggggcggca ggggcagggc ggtcgtacgc atctggtgag
     2881 tccggagatg gcggctgctg ctgccatcgc agggcatttc gtggatgtca gcaagctgtg
     2941 aggagttcaa cggtgaaaaa aatttcgatg ctgttgttga tgctgatcgc agttgccggg
     3001 aatgttgcat cggccgctga agaaggcgtg gtggacaagg cgggtcacgg catccagaaa
     3061 ggtggcgagg ctgccgggcg gggtatcgaa aaaggggccg atgcgacggg ggaagcgctt
     3121 aagaagggtg gcgaggcaac cgtcaagggc gtgaagaagg ctggcaaatg ggtcggcaaa
     3181 aagctgcaaa aaggcggcga aaaattagag aaggcgagcg agtgatgcgt aagttctcat
     3241 tgctggatgg gctggtcgtg ccgttggatc gagccaacgt agacaccgat gcaatcatcc
     3301 cgaaacaatt tctgaagtcg atcaaacgtt ccggcttcgg cccgaatgca ttcgacgagt
     3361 ggcgttatct cgatcacggc gagccgggaa tggactgcag caagcgtccg gtcaatccgg
     3421 acttcgtgct gaaccagccg cgctatcagg gtgcgcagat tctgctggcg cgcgaaaact
     3481 tcggctgcgg ctcctcgcgc gaacatgcgc cctgggcgct ggaagactac ggcttccgcg
     3541 tgatcatcgc gccgagcttc gccgacatct tcttcaacaa ctgcttcaag aacggcctgc
     3601 tgccgatcaa actggatgcg gacaaggtcg atgcgctgtt caaggcggtg gaagccaatg
     3661 tcggctacaa gctgaaggtc gatctggagc agcagaccat cacgttgccg gacggcacga
     3721 cataccagtt cgacgtgg
//
LOCUS       DASUIV010000040         5861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_566969, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000040 DASUIV010000000
VERSION     DASUIV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566969"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFW59_01325"
     CDS             <1..930
                     /locus_tag="VFW59_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5362888.1"
                     /translation="FARSSPMQKLRIVKALQKKGEIVTVTGDGVNDAPALKNADMGVA
                     MGRSGTEVAKEAANMVLMDDNFATIVNAVEEGRTVYCNIKKFIAYILTHLVPEMLPFI
                     AFIALGAPLALTVVLILSIDLGTDLLPALGLGREPPEHDVMKQPPRRRDERLLSWPLL
                     TMSYGIVGMLQAAAGFFAFFVVLHQGGWVWGSALPVSGLLYKTAVTSFFAAVVICQAA
                     NVLICRTRRQSLLSAGIFANKLIWAGIAVELALVAAISHVAFLQPFFGTAPIGWFEVS
                     LGLPFAAAILLGDEFRRWLIRRDNRFVQRWLCW"
     gene            1115..2458
                     /locus_tag="VFW59_01330"
     CDS             1115..2458
                     /locus_tag="VFW59_01330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5362889.1"
                     /translation="MNTPLTAPASRVSGCDVLVIGGGPAGSTAAALLAERGYRVTLLE
                     KAHHPRFHIGESLLPANLPLLEKLGVAEAVKAIGMEKWGAEFVSPWHEHRQSFEFADA
                     MDKSMPMAYQVRRAEFDEILIRNASRKNARVVEGCQVQEVDFLPDDAGALVQARHDDG
                     SLETLHARFVLDASGRDTFLGNRFKAKQRNKKHNSTAIYAHFSGAERNPGKAAGSITI
                     FWFEHGWFWFIPLIDGTTSVGAVTWPYYLKTRKEKPLAQFFQETIAMCAPLSERLRHA
                     QLSSPVEATGNFSYVCDRTHGSNYLLLGDAYAFIDPVFSSGVMLAMQSAFVGADTVDT
                     CLRHPQQAAAALKKFDRTMRHGPKLFSWFIYRVTNPTMRNMFMAPRNIFRVQEALLSV
                     LAGDVYGKTPIHGPLRIFKGLYYASSLFNFRRTLKSWRMRKHNIRVVAETAVLES"
     gene            2458..3522
                     /locus_tag="VFW59_01335"
     CDS             2458..3522
                     /locus_tag="VFW59_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362890.1"
                     /translation="MRDRSSQLHDASLSVAACLDRPESWQRGVLGALCFSVDAAESPL
                     LADLGMPCLRVPMPRLDAVDSSCEVWHGSGRLTSGQCGDIRYRHDEEMLFGVIVLPET
                     LFSAEPDSTPLQQATESAYRQIFALLDSLHFPYLFRFWNYIADINACSFGLERYRQFN
                     RGRQDAFLAHGRDVLGNVPAACALGFARQNSAQGELTIAFLAGRVAPLNIENPRQISA
                     YQYPQQYGPRSPTFSRASLVRMGQDEVLFVSGTASIVGHATLHARDVVAQTRETMANI
                     EAVLGEANRRAARFELADLSYRVYLRHPADLAQIRAELVRCVGGEINAVYLQADVCRE
                     DLLMEIEATAVHPFVTGERG"
     gene            3525..4322
                     /locus_tag="VFW59_01340"
     CDS             3525..4322
                     /locus_tag="VFW59_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5362891.1"
                     /translation="MRVVRVLLNGYDYLVLYLGLIWLGMLCLAWTPVALVIGPLLPER
                     QGRSLGRYVITMAFRFYLACLGASRRCSFDLTALDALRDEPSLILAPNHPCLLDAVMV
                     ISRLPNVACVLKADLMNNVFLGAGARLARYIRNDPVRRMVQQAMGDFESGSHLLLFPE
                     GTRTTSSPVNPFKGSIGLIAHRAQVPVQTILIETDSAYLSKGWPLFRKPVMPIHYRIR
                     LGRRFDPPQHTQRFMDELQHYFAHELVQGSAFYPTNALSTQADRTPP"
     gene            4319..5083
                     /locus_tag="VFW59_01345"
     CDS             4319..5083
                     /locus_tag="VFW59_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5362892.1"
                     /translation="MTATSTTHLVLIPSYNPGAKVYETVRAARQHWAPVWVVVDGSTD
                     GSAEGLLAMAAQDDGLRVMVLPRNQGKGAAVLHGLEQATAAGFSHVLTMDSDGQHPPG
                     LIPAFMAESLRQPSAMILGVPVFDASAPSLRVKGRRVSNWWANLETLWAGIGDSLFGF
                     RVYPVEPLRQVMRRQPWMRHFDFDPEAVVRLCWRGVRPVNLLAPVRYYLAEEGGVSHF
                     RYLRDNLLLTWMHTRLFFGFVLRLPVLLLRRARGRS"
     gene            complement(5099..>5861)
                     /locus_tag="VFW59_01350"
     CDS             complement(5099..>5861)
                     /locus_tag="VFW59_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5362893.1"
                     /translation="AVTALPILILLMEKLEILRLPIGQRILRYASLDDIAIWGVLALI
                     LMDWERVGKQLGFLLAFGIASYLFRKLMRWMPERDRWFAALIWLTVCGFGADWSGLHF
                     MVGAFIAGAVMDSEWFDQKQMDLLRHHVFLLLMPVFFLSTGLRTNWAVGGSAVFIVAA
                     ILLFASVSGKLIGIHAAGKILNWKPGEASIIGWLLQTKALIMIVFVNVLLDKGIITSE
                     TFTALLLMAVASTMLTVPMASPKLARMKEIIFRSK"
BASE COUNT         1060 a         1757 c         1827 g         1217 t
ORIGIN      
        1 ttcgcgcgct cgtcgccgat gcagaagctg cgcatcgtca aggcgctgca gaaaaaagga
       61 gagatcgtca cggtcaccgg cgatggcgtc aacgatgcac cggcattgaa gaatgcggac
      121 atgggggtgg cgatggggcg atccggcacc gaggtggcga aggaggcagc taatatggtg
      181 ttgatggacg acaacttcgc caccatcgtc aacgccgtcg aagaaggtcg taccgtttat
      241 tgcaacatca agaaattcat cgcctatatt ctgacccact tggtccccga gatgctgccc
      301 ttcatcgcgt tcatcgctct gggcgcgccc ttggcgctga cggtcgtgct gatactgagc
      361 atcgatctgg gtaccgatct gttgccggct ctggggctgg gacgcgaacc gcccgagcat
      421 gacgtgatga aacagccgcc gcgcaggcgc gatgaaaggc tgctcagctg gccgctgctc
      481 accatgagtt acggcatcgt cggcatgctg caggcggcag ccggattctt cgccttcttc
      541 gtcgtgctgc atcagggcgg ctgggtctgg ggcagcgcat tgcctgtgtc gggcctgctc
      601 tacaagaccg ccgtgacttc gttcttcgcc gcagtggtca tctgtcaggc agccaatgtg
      661 ctgatctgcc gcacccggcg gcagagcctc ctgtctgcag ggatattcgc caataaactg
      721 atctgggccg ggatcgcggt cgagctcgcg ctggtggccg cgatttccca tgttgcattt
      781 ttgcaaccct tcttcggaac ggcaccgata ggctggttcg aagtctcgct cggtctgcct
      841 tttgccgccg cgattctgct gggcgacgaa ttcaggcgct ggctgatcag gcgggacaac
      901 cgttttgtgc aacgctggtt atgctggtag cgcgcgatac gccggtgtcg agcgggtttt
      961 aaatcggccg gtatcgtctg cacgagcatg acggccgtcg ttgcggctgg aggaataacc
     1021 gggtgagcct gtggcgcgac ccataaatca atcatgatcg tcggatggat acgatacaat
     1081 ttggattgat gaaaaacagg ccagagctca agccatgaat acacccctta ccgcaccggc
     1141 ctctcgggtt tcgggttgtg acgtactggt gatcggcggc ggtccggcag gctcgaccgc
     1201 cgccgcgttg ctggcagagc gcggctatcg cgtgacgctg ctggagaaag cgcaccatcc
     1261 gcgttttcac atcggtgaat cgctgctccc cgccaatctg ccgctgctgg aaaagctggg
     1321 cgtggccgag gccgtcaagg ccatcggtat ggaaaaatgg ggcgcggaat tcgtctcgcc
     1381 ctggcatgag cacagacagt cgttcgagtt cgccgatgcc atggacaaat ccatgcccat
     1441 ggcctatcag gtgcgccgcg ccgagttcga cgagatcctg atccgtaacg ccagccgcaa
     1501 gaatgcccga gtggtggaag gctgccaggt tcaggaagtc gacttcctgc ccgacgatgc
     1561 cggtgcgctg gtgcaggcgc gtcacgatga cggcagtctc gaaacgctgc atgcgcgttt
     1621 cgtgctggat gcgtcggggc gcgacacctt tctgggcaat cgcttcaaag ccaaacagcg
     1681 caacaagaag cacaacagca ccgcgatcta tgcccacttc tccggggccg agcgcaaccc
     1741 gggcaaggct gcgggcagca tcacgatctt ctggttcgag catggctggt tctggttcat
     1801 tccgctgatc gacggcacga ccagcgtcgg cgcggtgacc tggccgtatt acctgaagac
     1861 gcgcaaggaa aaaccgctcg cccagttttt tcaggaaacc atcgccatgt gcgcgccgtt
     1921 gagcgagcgg ctgcggcatg cgcaactgag ttcgccggtg gaagcgaccg gcaatttctc
     1981 ctatgtctgc gaccgcaccc acggcagcaa ctatctgctg ctgggcgatg cctatgcgtt
     2041 catcgatccg gtgttctctt ccggcgtgat gctggccatg cagagcgcct ttgtcggagc
     2101 cgataccgtg gacacctgtt tgcgccatcc gcagcaggcg gcggccgcct tgaaaaaatt
     2161 cgaccgcact atgcggcatg gcccgaagct gttttcttgg ttcatctatc gagtcaccaa
     2221 tcccaccatg cgcaacatgt tcatggcgcc gcgcaatatt ttccgcgtac aggaggcgtt
     2281 gctgtcggtg ctggccggag acgtgtatgg caaaacgccg atccatggcc cgctgcggat
     2341 attcaagggg ctgtattacg cgtcgtcctt gttcaatttc aggcgcacct tgaagtcctg
     2401 gcggatgcgt aaacacaata tccgggtcgt ggctgaaacg gctgtgctgg agagctgatg
     2461 cgcgacaggt catctcagct gcatgacgcc agtctgtctg ttgccgcgtg tctggatcgg
     2521 ccggaatcgt ggcagcgcgg cgtgctcggc gcgctgtgct tttctgtcga tgccgccgag
     2581 agcccgctgc tagccgacct ggggatgccc tgtctgcgcg tccccatgcc gcgactggat
     2641 gccgtcgatt caagctgcga agtctggcat ggcagcggaa ggttgacctc gggacagtgc
     2701 ggcgatatcc gttaccgtca tgacgaggag atgctgttcg gcgtgatcgt cctgcctgaa
     2761 acgctgtttt cggccgagcc tgacagcacg ccgctccagc aggccaccga gtcggcctat
     2821 cgtcagatat tcgccttgct cgattcactg cacttcccgt acctgtttcg tttctggaat
     2881 tacatcgccg atatcaatgc ctgcagtttc ggtctggagc gctatcgcca gttcaatcgg
     2941 gggcggcagg atgctttcct cgcgcacgga cgcgatgtgc tggggaacgt ccccgccgct
     3001 tgcgcgctgg gtttcgctcg gcaaaattcg gcccagggcg agttgaccat cgcctttctg
     3061 gccggacgcg tggcgccgct gaacatcgaa aatccgcgcc agatcagcgc ctatcaatat
     3121 ccgcaacaat acggcccgcg cagcccgacg ttttcccgcg ccagtctggt gcgcatgggg
     3181 caggacgagg tgttgttcgt ctccggcacc gccagtatcg tcggtcacgc caccttgcat
     3241 gctcgcgatg tcgtggcgca aacccgcgag accatggcca acatcgaagc cgtgctgggc
     3301 gaggcgaatc gtcgggcagc ccggttcgaa ctggccgatc tgtcttaccg ggtctacctg
     3361 cgtcatcccg ccgatctggc gcagattcgc gccgagctcg tccgctgcgt gggcggtgaa
     3421 atcaatgcgg tctatctgca ggccgatgtg tgccgcgagg atctgctgat ggaaatcgaa
     3481 gccacggcgg tacatccgtt cgtgaccggc gagcggggct gaggatgcgc gtcgtgcgtg
     3541 tattgctgaa cggctacgat tatctggtgc tgtatctcgg cctgatctgg ctggggatgc
     3601 tgtgtctggc gtggacgccg gtcgcgctgg tcatcggccc gttgctgccc gagcggcagg
     3661 ggcgctcgct cgggcgctac gtcatcacga tggctttccg gttctatctg gcctgtctgg
     3721 gcgcatcgcg ccgctgcagc tttgacctga cggcgctgga tgcgttgcgc gacgagccgt
     3781 cgcttattct cgcgcccaac catccttgtc tgctggatgc ggtgatggtg atctcccgcc
     3841 tgccgaatgt cgcctgtgtg cttaaggccg atctgatgaa caacgtcttt ctcggcgcgg
     3901 gtgcccggct ggcgcgctat atccgcaatg atccggtgcg gcgcatggtg cagcaggcta
     3961 tgggcgattt cgaatctggc agccacctgt tgctgttccc ggaagggacc cgcaccacct
     4021 ctagtcctgt caatccgttc aagggcagca tcggcctgat cgcgcatcgc gcgcaggtgc
     4081 cggtgcagac gatcctgatc gagaccgact cggcttatct gagcaagggc tggccgctgt
     4141 tccgcaagcc ggttatgcct atccattacc ggatacgtct gggacgccgc ttcgatccgc
     4201 cgcaacatac ccagcgcttc atggatgaat tgcaacatta cttcgcgcac gagctggttc
     4261 aaggctcggc gttctatccg accaatgccc tgtccacgca ggctgaccgt acgccgccat
     4321 gaccgccacc tcgaccacgc atctcgtcct catccccagc tataaccccg gagccaaggt
     4381 ctatgagacc gtgcgtgccg caaggcaaca ctgggccccg gtgtgggtgg tggtggatgg
     4441 cagcaccgac ggcagcgccg aaggcctgct ggcaatggcc gcacaagacg acggtctgcg
     4501 cgtgatggtc ctgcctcgca accagggcaa gggcgcggcg gtgctgcatg gactggaaca
     4561 ggcgactgcg gcgggattca gccatgtgtt gaccatggac tcggacggcc agcatcctcc
     4621 cgggctgatc cccgcattca tggcggaatc cctgcgccag ccgtccgcga tgatcctggg
     4681 cgtgccggta ttcgatgcga gtgcgcccag cctgcgggtg aaagggcggc gcgtctccaa
     4741 ctggtgggcc aatctggaaa cgctgtgggc gggcataggc gactcgttgt tcggctttcg
     4801 cgtgtatccg gtcgagccct tgcggcaggt gatgcgccgc cagccgtgga tgcgccactt
     4861 cgacttcgat ccggaggccg tggtgcgcct gtgctggcgc ggtgtcaggc cggtcaatct
     4921 gctcgccccg gtgcgctact accttgcgga agagggcggc gtgtcgcatt ttcgctacct
     4981 gcgcgataac ctgctgttga cctggatgca cacgcggctg tttttcggct ttgtgctgcg
     5041 cttgcccgtt ttgctgctac gacgggcgcg gggacgatcc tgaatcaacc ggcctgcatc
     5101 actttgacct gaaaataatt tctttcatgc gagccagctt cggtgaagcc ataggcactg
     5161 tcagcatggt gctggcgaca gccatcagca gcagcgcggt gaaggtttcg ctggtgataa
     5221 tgcctttgtc caatagcaca ttgacgaaga caatcatgat cagtgctttg gtttgcaaca
     5281 gccaaccgat gatggaggct tcgcccggct tccagttgag tatttttccg gcggcgtgaa
     5341 tgccgatcaa tttccccgag acggaagcga acagcaagat tgcggcgacg atgaacaccg
     5401 ccgagccgcc cacagcccaa ttggtgcgca gaccggtact gagaaagaac accggcatga
     5461 gcagcagaaa cacatggtga cgcagcaagt ccatttgttt ctggtcgaac cattcgctgt
     5521 ccatcaccgc accggcaatg aatgcgccga ccatgaagtg caagccggac cagtcggcac
     5581 cgaatccgca tacggtcagc cagattagcg cggcaaacca gcggtcgcgt tccggcatcc
     5641 agcgcatgag tttgcggaag agataactgg cgataccgaa agccagcaaa aatccgagtt
     5701 gtttgccgac gcgttcccag tccatcagga tcagcgcgag cacgccccag atggcgatgt
     5761 cgtccagact ggcataacgc aggatgcgct gaccgatggg tagtctcagg atttccagtt
     5821 tttccatcag caagatcaga atcggcagcg cggtgaccgc g
//
LOCUS       DASUIV010000041         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_102399, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000041 DASUIV010000000
VERSION     DASUIV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102399"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..>3298)
                     /locus_tag="VFW59_01355"
     CDS             complement(112..>3298)
                     /locus_tag="VFW59_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5362894.1"
                     /translation="ELEWGMVVKVDVDEVLEPIARQRTLMIEALAVCLLFAALTAYYL
                     GRRISRPIRALAQVVDQVSAGRLDSRADENAPGELGVFAAAFNRMTVNLQELYRTLED
                     RIDERTRELNATNEQLQEEVHERMQSEITLANSNNELLLYKRFMDASGNAMAMTSLDG
                     LLTYGNPALFEMFGLAEDQIAGCIGTCNLHEHFRPEDAARMQAEGAREGSWSGEVEMT
                     NRMGRHFEAQVERFVVKDSIGEVYALAYVIVDISLRRQADLLMQRYKVVLETAQDGYW
                     VTDMQGNVLEANAAYARMSGYSIDELTTMHVSQLDVWDGPEAVRARVEKIVAEGSGRF
                     ESRHRRKDGGEIDIEVSVTHEPESQNLFVFCHDITERKAAQLEVQHQRDLLDEAQRLG
                     QLGSWELNLVSGELYWSDEIYRLFELDPAQFEPSYENFLNVIHPDDRDKVNQAYAQSL
                     IDRQSYDVEHRLLMPDGRIKWVREHCTSDFDEAGKALRSVGAVQDITASKYAEDQLRI
                     AAATFETHEAIMITDADANILRVNRAFEETTGYSAEEVQGKNPRILSSGHQDKDFYAE
                     MWQELSGSGTWSGEMWDRRKDGQIYPKWLTITAVRNEQGTITEYVAIFSDITERKQAE
                     EEIRNLAFYDALTKLPNRRLLLDRFHQALALSVRSQAYGAVLFLDMDRFKVLNDTLGH
                     DYGDLMLIEVAERITNCVRDVDTVARLGGDEFVVLIEETGRELDETSRRIAVIAEKIR
                     WSLAQPYRLKDSVHHSSPSIGVSLYRGDEQSVDTLLKQADMAMYRAKESGRNTVRFFD
                     PDMQHAVETRATLETDLRLAIANRELHLYYQIQVDKDFRPLGVEALARWLHPTRGMVP
                     PGQFIPIAEESSLILDIGAWVLETACRQLALWAHSKVTRELGISVNVSAAQFRQPDFV
                     EQIAALLREHKISPARLKLELTESVVMNDVADVVVKMHALKALGVKLSMDDFGTGYSS
                     LSYLKQLPLDQIKIDQSFVRDIATDSNDAVMVRTIIDLAQNFGLDVVAEGVETEVQHR
                     FLRSHGCPAYQGYLFSKPVPVEALEKSLGGL"
BASE COUNT          642 a         1104 c          866 g          686 t
ORIGIN      
        1 atgctgcggc gatgatgaaa atcgcagtca ggcatgtcac ctccatcaac aaaggatatt
       61 gtaggggcac ggcgtgccgt gccccctcag cccattaaat ccgccgtgcc cctacagccc
      121 gcccagcgac ttctctaacg cctcgaccgg aaccggcttg ctgaacagat agccctgata
      181 ggccgggcaa ccgtgcgacc tcaggaacct gtgctgcact tcggtttcca ccccttctgc
      241 gacgacatcc agaccgaagt tctgcgccag atcgatgatg gtgcgcacca tcacggcatc
      301 gttgctgtcg gtggcgatat cgcgcacgaa gctctggtcg atcttgatct ggtcgagtgg
      361 cagctgcttc agataggaca gcgaagagta accggtgccg aaatcatcca tcgacaactt
      421 gaccccgagc gccttcaacg catgcatctt caccaccaca tcggcgacat cgttcatcac
      481 cacgctctcg gtcagctcca gcttcagccg tgccggactg atcttgtgct cgcgcaacag
      541 cgcagcgatc tgttcgacga aatcaggctg acggaactgc gctgcactga cattgaccga
      601 gatgccaagc tcgcgcgtga ccttggaatg agcccataag gcaagctggc ggcaggcggt
      661 ctccagcacc caggcgccga tatccaggat cagcgaactc tcctcggcaa tcggaatgaa
      721 ttgtcccggc ggcaccatgc cgcgcgtcgg gtgcagccag cgcgccagcg cctcgacgcc
      781 gagcggacga aaatccttgt ccacttgtat ctggtaatac aggtgcagct cccgattggc
      841 gatcgccagt cgcagatcgg tctctagcgt ggcacgcgtc tccacggcgt gctgcatgtc
      901 aggatcgaag aaacggaccg tattgcggcc ggattccttg gcgcgataca tcgccatatc
      961 cgcctgcttg agcagcgtat cgaccgattg ctcatcgccc cggtacagac tcacgccgat
     1021 gctgggcgaa ctgtgatgca cgctatcctt caaccggtac ggttgcgcca acgaccacct
     1081 gatcttctcg gcgatcacgg cgatcctgcg cgaagtctcg tcgagctcgc ggcccgtctc
     1141 ttcgatcagc accacgaact cgtccccgcc cagtctggcg acggtatcca catcccgcac
     1201 acaattggtg atgcgctcgg ccacttcgat cagcatcagg tcgccataat cgtgccccag
     1261 cgtatcgttc aacaccttga atcgatccat gtcgaggaac agcacggcgc cgtaggcctg
     1321 actgcgcacc gacaacgcca gcgcctgatg gaagcgatcc agcaacaggc ggcgattagg
     1381 caatttggtc agtgcatcat agaacgccag attgcggatc tcctcctcgg cctgtttgcg
     1441 ttcggttata tcgctgaaga tcgccacgta ttcggtaatc gtgccttgtt cgttcctgac
     1501 cgccgtgatg gtcagccact ttggatagat ctgcccatcc ttgcgccggt cccacatctc
     1561 tccggaccag gttccgcttc ccgacaattc ctgccacatc tcggcataga aatccttgtc
     1621 ctgatgcccc gagctcagga tgcgcggatt cttgccctgc acctcttccg cgctgtagcc
     1681 ggtggtctcc tcgaaggcgc gattgacccg caggatgttg gcgtcggcat cggtgatcat
     1741 gattgcctcg tgcgtctcga aggtcgccgc cgcgatgcgc aactgatcct cggcatattt
     1801 actcgcggtg atatcctgca ccgcgccgac cgagcgcagc gccttgcccg cctcatcgaa
     1861 atcgctggtg cagtgttcac gcacccactt gatgcgccca tccggcatca acaggcgatg
     1921 ctccacatcg taagactgcc tgtcgatcaa tgactgcgcg tacgcctgat tgaccttgtc
     1981 ccggtcatcc gggtggatca cattcaggaa gttctcgtag gacggctcga actgcgccgg
     2041 gtccagctcg aagaggcggt agatctcatc cgaccagtac aactcgccgc tcaccagatt
     2101 cagttcccag ctgcccagct gtcccagccg ttgcgcctca tcgagcagat cgcgctgatg
     2161 ctgcacctcg agctgtgctg ccttgcgctc ggtgatgtca tggcagaaga cgaacagatt
     2221 ctgcgattcg ggctcgtggg tcaccgagac ttcgatgtcg atttctcccc catccttgcg
     2281 acgatggcgc gattcgaagc gtccggaacc ttctgcgacg attttctcca cacgggcgcg
     2341 cacggcctcc ggcccatccc atacatccag ctgactgaca tgcatcgtcg tcaattcatc
     2401 gatcgagtag ccggacatcc gcgcataggc tgcattcgcc tcaagcacat tgccctgcat
     2461 atcggtcacc cagtaaccat cctgcgcggt ttccagcacc accttatagc gctgcatcag
     2521 caggtcggcc tgtctgcgca aggagatgtc gacgatgaca taggccaacg cgtaaacctc
     2581 tccgatcgag tccttcacaa cgaacctttc gacctgcgcc tcgaaatgcc gtcccatgcg
     2641 gttcgtcatc tcgacctcgc cactccagct gccctcgcga gcgccttccg cctgcatccg
     2701 cgcggcatcc tctggcctga aatgttcatg gagattgcat gttccgatgc agcctgcgat
     2761 ctgatcctcg gccaatccga acatttcgaa cagtgcagga ttgccatagg tcaacaaacc
     2821 gtcgagcgaa gtcatcgcca tcgcgttacc ggatgcgtcc atgaaacgct tgtacagcaa
     2881 caactcgtta ttgctgttcg ccagcgtgat ctcggactgc atacgctcgt gaacttcttc
     2941 ctgcaactgc tcgttggtcg cgttcagttc gcgggtgcgt tcgtcgatac ggtcctccag
     3001 cgtccggtac agctcctgaa ggttgaccgt catccggttg aatgccgcag caaacacgcc
     3061 cagttcgccg ggcgcattct cgtccgcgcg actgtccagc ctgcccgcag acacctgatc
     3121 caccacctgc gccaaggccc tgatcggcct gctgatgcgg cgaccaaggt aataggcggt
     3181 cagtgcggca aacaacaggc agaccgccag cgcctcgatc atcagcgtcc gctgccgggc
     3241 gatcggctcg agcacctcgt ccacatccac cttgacgacc atcccccact ccagttcc
//
LOCUS       DASUIV010000042        16951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1125136, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000042 DASUIV010000000
VERSION     DASUIV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 16951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16951
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125136"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFW59_01360"
     CDS             complement(<1..276)
                     /locus_tag="VFW59_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362895.1"
                     /translation="MNQTIGAGIITYEADIWKTADTLRGCAIKESDWPGYMMPFFALM
                     LVESRVRRLRTEKIAEYQEATGEKFDEGNTEHITWLDMSVKAAGKGYH"
     gene            complement(337..2361)
                     /gene="uvrB"
                     /locus_tag="VFW59_01365"
     CDS             complement(337..2361)
                     /gene="uvrB"
                     /locus_tag="VFW59_01365"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX5362896.1"
                     /translation="MSVITFPSSPFLLHQPFEPAGDQPAAIAKLVEGLEDGLAYQTLL
                     GVTGSGKTFTMANVIARTGRPAIIMAPNKTLAAQLYSEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDVFIEKDSSVNEQIEQMRLSATKALLERPDAVIVATVSSIYGIGDPVDY
                     HGMILHLRHNERIPQRDVIKRLTEMQYERNDVDFTRGTFRVRGDVIDIFPAESSEHAL
                     RLSMFDDEVEGLSLFDPLTGHMLQKVPRYTVYPSSHYVTPRSTVLRAIETIKLELAER
                     IAFYQRENKLVEAQRIEQRTRHDLEMLNEIGFCKGIENYSRHLSGRPAGAPPPTLLDY
                     LPPDALMFLDESHVMIPQVGGMYKGDRARKENLVNYGFRLPSAMDNRPLRFDEFEKVM
                     HQAIFVSATPAVYEAEHAGQVVEQVVRPTGLIDPVIEVRPATNQVDDLMSEVRLRVAQ
                     GERVLVTTLTKRMSEQLTDYFTEHGIKVRYLHSDIETVERVEIIRDLRLGMFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARHLNGKALLYADRITDSM
                     RRAIDETDRRRKKQQAHNEAHGITPIGVQKRIKDIIEGMYEPDAARSQLKAAQAQAKY
                     LAMSEKEVAKEIARLEKEMMQAAKNLEFERAAELRDQLRKLKEAVFLSPL"
     gene            2504..3838
                     /locus_tag="VFW59_01370"
     CDS             2504..3838
                     /locus_tag="VFW59_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5362897.1"
                     /translation="MSTFADLKLAPEILRALTESGYTSPTPIQEQAIPLVLEGNDLMA
                     GAQTGTGKTAAFALPMLQKLLPHASPSTSPARHPVRALILVPTRELAIQVEENVKAYA
                     KHTRLRSLALFGGVDIKTQTPHLKNGVEILVATPGRLLDHIEQKTLQLNTVQMLVLDE
                     ADRMLDMGFMPALKRILALLPRQRQSLMFSATFSDEIKKLSEEFLIYPTLIEVARSNA
                     SAENIAQKVYLVEQENKHALLTQLLRGDDAKQVIVFTKTKLTASRLAHQLQREGVAAD
                     AIHGDKSQTERMQALEAFKQGSVTVLIATDVAARGLDIDSLPMVINYEIPHAAEDYVH
                     RIGRTGRAGASGTAISLVSPEEERYLLDIEKLIKKEIQRETLPELAKPQRAPRPEQTH
                     EYHRPAAKKPSHDAWFDKPYVPNITAQTVDAKPEEALNKPKTQIPALFRRPG"
     gene            3887..4219
                     /locus_tag="VFW59_01375"
     CDS             3887..4219
                     /locus_tag="VFW59_01375"
                     /inference="COORDINATES: protein motif:HMM:NF018644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEX5362898.1"
                     /translation="MATLILFNPLPSAVLWFYLITSSIAFLAYALDKSAARNRQWRIR
                     ESTLHLLGLIGGWPGAFAAQRLLRHKSGKPSFQILFWITVALNCGALGWIGLSPDAGT
                     LRALLDAA"
     gene            4312..5505
                     /locus_tag="VFW59_01380"
     CDS             4312..5505
                     /locus_tag="VFW59_01380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5362899.1"
                     /translation="MSLSTRVQAIKPSPTLAVAARAAKLKAEGRDIIGLGTGEPDFDT
                     PQHIKDAAIGAINKGFTKYTAVGGTPSLKQAIIAKLKRDNGLDYTAKQILVSCGGKQS
                     FFNLVLATIDPGDEVIITAPYWVSYPDIVIIAEGKPVIVQADIAQGFKLTPAQLAAAI
                     TPKTKMVVINSPSNPSGAMYTLDDLKALGEVLRKHPNILIATDDMYEHIQLGDDKFCN
                     ILNACPDLYPRTMVLNGVSKAYAMTGWRIGYAAGPENIITAMENVQSQSTSNPTSISQ
                     VAAEAALNGDQNCLAPMIKAFRERHVFVVNELNKIPGVKCLMAGGAFYAFPDARGAIS
                     TLHKQGRIKEATDIALSEYLLAEAGVAVVPGSAFGSEGYIRLSFATSMENLKNALERI
                     SKALS"
     gene            5795..6655
                     /locus_tag="VFW59_01385"
     CDS             5795..6655
                     /locus_tag="VFW59_01385"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5362900.1"
                     /translation="MTTQQKDIASRVLAALESGQTELPTLPDIALKLRDLLQDSDVAV
                     NRIVSLLSTDPVISMHVIRAANSAALFRGEPVFNLHDAVLKLGNQMLHSMIMNITLNK
                     LFQARSPLIDRTLKQLWEHSHAVAANSYVLAREQAHLRPDTAMLIGLVHGIGALPLYL
                     YADRHYPQIESATLDSLVGEHAAAITPRLLKNWSFPEELVEVVASQSAQKELDGADRA
                     SYSDVLAIAVLQADDPEATLSRRDMLAAERLGYYAADCRNFQPNHREQLASVKNLLRV
                     DVARSGKLQK"
     gene            complement(6671..10387)
                     /locus_tag="VFW59_01390"
     CDS             complement(6671..10387)
                     /locus_tag="VFW59_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362901.1"
                     /translation="MDNKSIFVKTDKGEDEIRSRTPHLSGDIKRALLMVDGKASLDEL
                     SKRAAPSMREMLQPLFVELESAGFIQNKAKVGKPHEAHKSANAPASGHAPKLSVPAGM
                     VVPAKKPVSTNLEELDFTAAYRVPSPAVLAAEAAKADAERLKKEIEIRAREEAEANAR
                     KEIEAAKQKAQQEAEAARIRAEQEAAKVRKEAELVKMKAQQDAEAARLKAEQEAAKAR
                     EEAEAAKRKAATEARAREQAEHQAREQAEAARLKAEQEAAQVRAELAAAKAQAEADAR
                     AHAEAEARARKEIEIAKLKAQEEAEAARLKAERAAAKAHEEAEQAKRQAAAAQAKVAA
                     EAKAREEAEYKAREQAEAARQKAEQEAAQAREEAELAKRQAAEIQAKIEAEARMRQEI
                     EAAKLKAQQEAEAARIKAEEDAEKARAEAAEQKAQAEAARLKAEQDAEKVRAEAAEQK
                     ARAEAERIKAEQDAAKMRAETEAANLKARQEAEAVRIKAQQEAEALRVKAEQEAEANA
                     PARKFEGGTAKRASSVSVTVLFFDVVGYTKQPVNKQIKIKKQFNQLVSDCLGTLEEAE
                     RIILDTGDGAAIGFLHHPEDALQVARQFREAVTAHRHTDYPELNVRTGIHLGPINIVK
                     DMNGQSNMVGDGINDAQRVMSFAGVDQIFISRPYYDFISRLSDEYANLFQSRGIKQDK
                     HGREHAVYELVDISEADVIAPVSQPVTAPIKLEPFVFAAPEIGTAPIQPVTPEEEHTQ
                     TSTLLGDAMAGAESKLPETNSGQVPPAPTKVEQAPPAKEEPKPVAKAHMPTEEEVATI
                     AAAQAKVWSEAEQRAAKASEERAAQAAQPVPPVEVPKVKARRKPIPWGKVAVGLCVLM
                     VVAVFVVPLVLPMQERAAKLEQMLATQFKQPVHIGKMSGRLLPTPRLELSDVSIGETK
                     QIRMKSARMDFSLSALFSSTKAINTLGLEGVQVDGAVLQQLPPWLQQLAGNAQYPVAQ
                     VELSQVSLETEGLKLSGIGGLVSFDAAGKFVQAKLHANEDKYALEINAAPQDKLKVTI
                     SVRNSAPPLLAGWVFEDLRANGELTRDELIISDLDASTMDGRLLGDARLSWGAGWHAT
                     GNLVAKAITLQKISNTLSGDMDGSARFRMQADSLGKLPDSSVLEGSFVATKGIINGFD
                     LVETARLRSKDSQPGGRSHFDELRGELTVDKGRYRFSQLRMTAGVLSASGTVDMEKQQ
                     LSGRITAELALRTGMGSVGLQVGGTVDNPSLHAR"
     gene            complement(10428..12143)
                     /locus_tag="VFW59_01395"
     CDS             complement(10428..12143)
                     /locus_tag="VFW59_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX5362902.1"
                     /translation="MKWFKSALVGVFALLMLAMLTVYLTPLDVYVPEVEQTLSARLQM
                     PVSVGSLRAAIFPWPHLELRDALIGGQEGIAVRSIMVSPNLSDLLAGQLVMHVELRDG
                     AAYFAPLRKLLEVLSHAPLVEQGPRLGEMWLSGVILLFPEMAVGPLEGKIEFTRTGAL
                     QRAWFALDEKKLTAVLTPLQNMGIAFQVRAKEWMSPPPVRLPLDILQLDGVFTGQGII
                     AQQFSVSSRGMHVAGSGNLNFSNGWKIHASVNQAEIQLDQLMALLGKPVGLSGVLSGK
                     GKLDGKASDWKLLAEELSFVGDLHAVNVKARIAESFGQPLAFDQIQSHVVLGPRQMEM
                     SGLTAVLYGGVLTGGMSIKHDNEMLNGQVSVHEINMSPLVKALTNEVLFSGKLDSEAK
                     FSMSLDRLDRFPENLMLTGNFHLRDGVLSKVDLVQAVSSQSKAEAKGGVTKFDDLTGT
                     INVNASGYHFRAIKISSGSLKADGRVDVAPTLQLNGVLDVDVKGTAGLVSMPVVVSGT
                     LNDPVVRVSSSVWAGAAMGTAILGPGLGTALGVKVGGFLNKLFGSHRDKNDAKEAYPN
                     KGEAK"
     gene            12205..12280
                     /locus_tag="VFW59_01400"
     tRNA            12205..12280
                     /locus_tag="VFW59_01400"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12238..12240,aa:Asn,seq:gtt)
     gene            12670..12831
                     /locus_tag="VFW59_01405"
     CDS             12670..12831
                     /locus_tag="VFW59_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362903.1"
                     /translation="MLKVFLTIDPQTLRGLYDDEAKLLGIFQSMSEAEDALGDMLNFC
                     TIHSESPNQ"
     gene            13314..13526
                     /locus_tag="VFW59_01410"
     CDS             13314..13526
                     /locus_tag="VFW59_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX5362904.1"
                     /translation="MQQFQLNGEFIELNQLLKMVGLCDSGGAGKALVAEGLVSVDGRV
                     ELRKTAKIRAGSVVSLGDVSISVLAA"
     gene            13854..14045
                     /locus_tag="VFW59_01415"
     CDS             13854..14045
                     /locus_tag="VFW59_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEX5362905.1"
                     /translation="MKYRHYKGGIYEIVCEARLEADPDVIMVVYKSEDGLIWTRPRDV
                     FFELVQHEGNTVQRFAPIN"
     gene            complement(14083..16044)
                     /locus_tag="VFW59_01420"
     CDS             complement(14083..16044)
                     /locus_tag="VFW59_01420"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX5362906.1"
                     /translation="MVSVQHSHSALANHQDADSWLDAVAAHFSADETALIRRAYERAA
                     SLYEGKAGPSGTPLLPHALGSAAILVHLNMDAEAIAAALLHAVPDCLDEWKEKLEADF
                     AAPVLGLVEGVARMEQIRQLGAGQGIGKADAARQLESLRKMLLAMVQDIRVVLIKLAE
                     RTQTLRELSGADEQLRRGIAQETQRIFAPLANRLGVWQLKWELEDLSVRYLEPELYKR
                     VAKLLDERRVDRERYIAELLALLERELARADIRAEVSGRPKHIYSIVNKMKRKQLDFD
                     ELYDVRAVRVLVDDVESCYAVLGLVHDLWQPVPGEFDDYIAHPKSNDYRSLHTAVVGP
                     RGLNVEVQIRTHEMHQHSELGVAAHWRYKEGGKSDAGFDEKIAWLRQILKWKSELAEL
                     SGQFREGLIQDRVYVLTPQGRIIDLPKGATPVDFAYALHTDLGHRTRGAKVNGSIVPL
                     GYPLQNGQRVEILTTKLGAPSRDWLSPALGYLQTSSARAKVRHWFRQQGLDDSVAQGR
                     ALLERELQRLGAVGINQEKIAQRLRFNKTEELLIALGRGKVTLRRASLAIQQEMPSRS
                     AEAVQHAAPVRSVVRQASPEGVLVAGVGNLMIRLAKCCTPSSPDAIVGYVTRDRGITI
                     HRHDCPFMRRVPDDRRDRVLDAQWGKLSP"
     gene            complement(16065..>16951)
                     /locus_tag="VFW59_01425"
     CDS             complement(16065..>16951)
                     /locus_tag="VFW59_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5362907.1"
                     /translation="RIDNFLFRHLKGVPKSHVYRILRAGEVRVNKKRVPQTYRLQLGD
                     LLRIPPIRVAEKAEAEFVPAAEFPILYEDDALLAINKPSGVAVHGGSGVSFGVIEQLR
                     RARPQAKLLELVHRLDRETSGVLLVAKKRTALTGMHEIMREGNSDKRYFALVLGQWKN
                     ARQHVKLPLHKFDTPQGEKRVMVREDGQQAHTIFSLQQSWDEFSLLEAELKTGRTHQI
                     RVHLSHLGFPIAGDDKYGDFARNRALMKQGLKRMFLHAHSISFNHPLTGEPLQIEAPL
                     PPDLQSFIARLDAGTVSL"
BASE COUNT         3406 a         5529 c         4554 g         3462 t
ORIGIN      
        1 atggtagccc ttgcctgcgg ctttgacgga catgtccagc caggtgatat gttcggtgtt
       61 tccctcgtcg aacttttcgc cagtcgcttc ttggtattcc gcaatctttt cggttctgag
      121 tctgcggacg cgcgactcga ccagcatcag cgcgaagaac ggcatcatgt agccgggcca
      181 gtcactctcc ttgatcgcgc agccgcgcag cgtgtcggca gtcttccaga tgtccgcttc
      241 gtacgtgatg atgcctgcgc ctatggtttg attcatttaa tgccttcaaa aaaacgtata
      301 gcccaatgcg gaagcattgc tagcagcatc ctgtgctcac agcggcgaga ggaacaccgc
      361 ctccttcaat tttctcagct gatcgcgcaa ttcggcggca cgctcgaact ccaggttctt
      421 cgctgcttgc atcatctcct tctccagccg ggcgatttcc ttggcgactt ccttctcgct
      481 catcgccaga tacttggcct gagcctgcgc ggccttgagc tggctgcgcg cggcatccgg
      541 ctcgtacatg ccttcgatga tgtccttgat gcgcttctgc acccctatcg gcgtgatgcc
      601 gtgcgcctcg ttgtgcgcct gctgcttctt acggcggcgg tcggtctcgt cgatcgcgcg
      661 gcgcatcgag tcggtgatgc ggtcggcata cagcaaggcc ttgccgttca gatggcgcgc
      721 ggcgcggccg atggtctgga tcagcgagcg ttccgaacgc aggaaacctt ccttgtcggc
      781 atcgaggatc gccaccagcg acacctcggg aatatccagt ccctcgcgca acaggttgat
      841 gccgaccagc acgtcgaaca tgcccagacg caggtcgcgg atgatctcga cgcgctcgac
      901 ggtctcgatg tcggagtgca gatagcgcac cttgatgccg tgctcggtga aatagtcggt
      961 caactgttcc gacatgcgtt tggtcagcgt ggtgaccagc acccgttcgc cctgtgcgac
     1021 gcgcagcctc acctcggaca tcaggtcgtc cacctgattc gtcgcgggac gcacctcgat
     1081 caccggatcg atcagcccgg tcggacgcac cacctgctcc accacctgcc cggcgtgctc
     1141 ggcctcgtaa accgcaggcg tggcggagac gaagatcgcc tgatgcatca ccttctcgaa
     1201 ttcgtcgaaa cgcaacggac ggttgtccat cgcggaaggc agccggaaac cgtaattgac
     1261 cagattctcc ttgcgcgcgc ggtcgccctt gtacatgccg ccgacctgcg gaatcatcac
     1321 atggctctcg tccagaaaca tcagcgcgtc cggcggcaga tagtccagca gcgtcggcgg
     1381 cggcgcgcct gccgggcgcc ccgacagatg ccgcgagtag ttctcgatgc ccttgcagaa
     1441 accgatctcg ttgagcatct ccagatcgtg gcgggtgcgc tgctcgatgc gctgcgcctc
     1501 caccagtttg ttctcgcgct gatagaacgc gatgcgctcg gccagctcca gcttgatggt
     1561 ctcgatggcg cgcaacaccg tgctgcgcgg cgtaacgtaa tggctggagg gatagacggt
     1621 atagcgcggc accttctgca gcatgtggcc ggtgagcgga tcgaacagcg acagtccttc
     1681 cacctcgtcg tcgaacatcg acaggcgcag cgcatgctcg ctgctctccg ccgggaagat
     1741 gtcgatcacg tcgccgcgca cgcggaaggt gccgcgcgtg aagtcgacat cgttgcgctc
     1801 gtactgcatc tccgtcagac gcttgatcac gtcgcgctgc gggatgcgct cgttgtggcg
     1861 cagatgcagg atcatgccgt ggtaatccac cggatcgccg atgccgtaga tcgacgatac
     1921 cgtcgccacg atcaccgcat cgggccgctc cagcagcgcc ttggtcgccg acagccgcat
     1981 ctgttcgatc tgctcgttga cgctggaatc cttctcgatg aacacatcgc gcgacggcac
     2041 ataagcctcg ggctgatagt agtcatagta ggagacgaaa tactcgaccg cattctcggg
     2101 gaagaattcg cgcatctcgg aatacagctg cgccgccagc gtcttgttcg gcgccatgat
     2161 gatggccggt cgcccggtgc gcgcgatgac gttcgccatc gtgaaggtct tgcccgaccc
     2221 ggtcaccccg agcagcgtct ggtacgccag cccgtcctct agaccctcga ccagctttgc
     2281 gatggccgca ggctgatccc ctgccggttc gaacggctgg tgcaggagga agggactgct
     2341 gggaaaagtg ataacgctca tattcaaccg cgcattgtac gggtaaagag gcgattgcca
     2401 aaccgcagca caggatacgc aatcgttggc cgccaaccgg aagacccacc cggcgtataa
     2461 tggcgcccaa ccccatgatt gatatcccgg aagagagctg acattgagta catttgccga
     2521 tctgaaactc gcgcctgaaa tactcagagc cctcaccgag tccggttaca cctcccccac
     2581 ccccattcag gagcaagcca ttccactggt gctggaaggg aacgacctga tggccggtgc
     2641 gcagaccgga accggcaaga ctgccgcatt cgcattgccc atgctgcaga agctgctgcc
     2701 gcatgccagc cccagcacct cgccggcacg ccatccggtg cgcgccctca ttctggtgcc
     2761 gacgcgggaa ctggcgatcc aggtcgaaga gaacgtcaag gcctacgcga aacataccag
     2821 gctgcgttcg ctggcgctgt tcggcggcgt ggacatcaag acccagacgc cccatctgaa
     2881 gaacggcgtg gagatactgg tggcgacgcc ggggcgcctg ctggatcaca tcgagcagaa
     2941 gacgctgcaa ttgaacacgg tgcagatgct ggtgctggac gaggccgacc gcatgctgga
     3001 catgggcttc atgcccgcgt tgaagcgcat cctcgcgctg ctgcccagac agcgccagag
     3061 cctgatgttc tccgcgacct tctcggacga gatcaagaag ctgtcggaag aattcctgat
     3121 ctacccgacg ctgatcgaag tcgcgcgcag caatgcctcc gccgagaaca ttgcgcagaa
     3181 ggtctatctg gtcgagcagg aaaacaagca tgccctgctc acgcagttgt tgcgcggcga
     3241 cgacgccaaa caggtcatcg tgttcaccaa gaccaagctc accgccagcc gtctggccca
     3301 ccagttgcag cgcgaaggcg ttgccgccga tgccatccac ggcgacaaga gccagacgga
     3361 acgcatgcag gcgctggaag ctttcaagca gggcagtgtc accgtgctga tcgccaccga
     3421 cgtggccgcg cgcggcctgg acatcgacag cctgccgatg gtgatcaatt acgagattcc
     3481 gcatgccgcc gaagactacg tgcaccgcat cggccgaacc ggccgcgccg gcgcctccgg
     3541 caccgcgatc tcgctggtct ccccggaaga agaacgctat ctgctggata tcgaaaaact
     3601 gatcaagaaa gagatccagc gggaaacctt gcccgaactg gcgaagccac agcgcgcgcc
     3661 ccgccccgaa cagacccacg aataccaccg accggcagcg aaaaagccca gccacgacgc
     3721 atggttcgac aaaccctatg tgcccaacat caccgcgcag acagtcgatg cgaaacccga
     3781 ggaagcgctg aacaaaccga agacgcagat cccggcactg ttccgcagac cgggctgatc
     3841 gccggaaacc ccgaacgatc atgatgccgc ccgcttcctc gctctggtgg cgacgttgat
     3901 tcttttcaac cccttaccgt ctgcggttct ctggttctat ctcatcacca gcagcattgc
     3961 cttcctcgcc tatgcactcg acaagtcggc cgcgagaaac cgtcagtggc gcatccggga
     4021 gagcaccttg cacctgctcg gattgatcgg cggctggccc ggcgcattcg ccgcccaaag
     4081 gttgctgcgt cacaaatccg gcaaaccgtc ttttcaaatc ctgttctgga tcaccgtcgc
     4141 tctgaactgc ggcgccctcg gctggatagg tttgtccccg gatgccggaa cgcttcgcgc
     4201 cttgcttgat gcagcgtgag gactcagcga aattccccgc tgttccccga ttcactcttt
     4261 ccccggcata ggtataattc gccccaacgt ccaacccaat caggaaaaat cgtgtctctc
     4321 tccactcgcg ttcaagccat caagccttcc cctactcttg ccgttgccgc tcgtgccgcg
     4381 aaactgaaag cagaaggccg tgacatcatt ggactgggca ccggcgagcc ggatttcgat
     4441 acgccgcagc acatcaagga tgccgcgatc ggcgcgatca acaagggatt caccaagtac
     4501 accgccgtcg gcggcacgcc ctccctgaaa caggccatca tcgccaagct caagcgcgac
     4561 aatggtctgg actacaccgc gaagcagatt ctggtttcct gcggtggcaa gcagagcttc
     4621 ttcaatctgg tgctggccac catcgatccc ggcgacgagg tgatcatcac cgccccctac
     4681 tgggtctcct atccggacat cgtgatcatc gccgagggca aaccggtgat cgtgcaggcc
     4741 gacatcgcgc agggtttcaa gctgaccccg gcgcaactgg cagcggcgat cacgccgaag
     4801 acgaagatgg tcgtcatcaa cagcccgagc aatccgtcag gcgcgatgta tacgctggac
     4861 gacctgaagg cgctgggcga ggtgctgcgc aagcatccga acatcctcat cgccaccgac
     4921 gacatgtacg aacatatcca gttaggcgac gacaagttct gcaacattct gaacgcctgc
     4981 cctgaccttt atccgcgcac catggtgctg aacggcgtgt cgaaggcata cgcgatgacc
     5041 ggctggcgca ttggctacgc agccgggccg gaaaacatca tcaccgcgat ggagaacgtg
     5101 cagtcgcaga gcacctccaa cccgacttcg atctcgcagg tggccgccga agccgcgctg
     5161 aacggcgacc agaactgcct cgctccgatg atcaaggcgt ttcgcgagcg tcacgtgttc
     5221 gtggtcaacg agctgaacaa gattcccggc gtgaagtgtc tgatggcggg cggcgcgttc
     5281 tacgccttcc ccgatgcgcg cggtgccatt tccacgctgc acaagcaggg gcggatcaag
     5341 gaagcgaccg atatcgcgct atccgaatat ctgctggcag aagccggcgt tgcggtcgtg
     5401 cccggctcgg ccttcggcag cgaaggctat atccgcctgt cgttcgccac ctcaatggaa
     5461 aacctcaaga acgcacttga gcgcatatcg aaagcgttat cctgaggttt cggtgaagcc
     5521 acaatctcaa gaatgccctt gagcgcatct cgaaagtgct tgcttgagat ttcggcgaag
     5581 gctgagctac ccgtagcttc gaccaccgat ccggaacagg attgccctga cccaatcctc
     5641 gtgaagcgcc tgcggacaga cacggctgtc cgcaggcgcg cataggccga attacctata
     5701 tctttcggcc tatagtttta tgctcgatca actggtctaa tctgtgcctc gcggacatcg
     5761 gcgaatgact cgtcccctcc gccatgagca cactatgact acccagcaaa aagatatcgc
     5821 ctcccgcgta cttgcggcac tggagagcgg acaaaccgag ctacccaccc tcccggatat
     5881 cgcactcaaa ttgagagacc tgctgcagga ctccgatgtc gcggtgaatc ggatcgtctc
     5941 cctgctgtcc acggatccgg tcatctccat gcatgtcata cgcgccgcca acagtgccgc
     6001 attattccgt ggcgaacccg tcttcaacct gcacgatgcc gtcctcaaac tgggtaacca
     6061 gatgctgcac agcatgatca tgaacatcac actgaacaag ctgtttcaag ccaggagtcc
     6121 gctcatcgac aggacgctga aacaactgtg ggagcacagc cacgctgtcg cggcgaacag
     6181 ctatgtactg gcacgcgaac aggcccacct gagacctgat accgccatgc tgatcggact
     6241 ggtgcatggc atcggcgcat tgccgctgta cctctatgct gaccgccatt atccgcagat
     6301 cgaatccgcg acactggaca gtctggtggg cgaacatgcg gccgccatca caccgcgctt
     6361 gctgaaaaac tggagtttcc cggaggagtt ggtcgaggtc gttgccagcc aatcggccca
     6421 aaaagaactg gacggtgctg accgtgcaag ctattccgat gtgctggcca tagccgtcct
     6481 gcaagccgat gatccggaag cgacgctctc ccggagagac atgctggccg cagaacggct
     6541 aggttactac gcagcagact gccggaactt tcagcctaac cacagggaac aactcgcctc
     6601 cgtgaaaaac ctgttgcgcg tcgatgtggc aaggtcaggc aagctccaga aatagatgcc
     6661 ccctacccga tcaacgggca tgcaacgaag ggttatcgac ggttccgccg acctgaagtc
     6721 ccacagaccc catgccggta cgcagcgcca gctccgccgt gatgcgtccg gacagttgct
     6781 gtttttccat gtccacggtg cccgaagcgc tcagcacccc ggcggtcatc ctgagctgac
     6841 tgaaacgata acggccctta tcgacggtca gctcgcctct caattcgtcg aaatgactgc
     6901 gccctcccgg ctggctgtcc ttgctgcgca agcgagcagt ctcgaccaga tcgaaaccgt
     6961 tgatgatacc tttcgtggca acaaaggaac cctccagaac ggatgaatcc ggcaatttcc
     7021 ccagactgtc cgcctgcatc cggaaacgcg ccgagccgtc catgtcgcca ctcaaggtgt
     7081 tgctgatctt ctgcaacgtg atcgccttgg cgaccagatt acccgtggca tgccagcccg
     7141 cgccccagga cagacgcgca tctcccagca agcggccatc catcgtactg gcatccagat
     7201 cgctgatgat cagttcatcg cgggtcagtt cgccattcgc cctgagatcc tcgaagaccc
     7261 agcccgccag caacggcggc gcactgttgc gcaccgagat cgtcaccttc agcttgtcct
     7321 gcggcgcggc gttgatctcc agcgcatatt tgtcctcgtt ggcgtgcaac ttcgcctgca
     7381 cgaacttgcc cgcagcatcg aaactcacta gcccgccgat gccggagagt ttcagcccct
     7441 ctgtttccag actgacttga ctcagctcga cttgggccac cggatactgc gcattgcctg
     7501 ccaactgttg cagccaaggg ggcagttgtt gcagcaccgc accgtctacc tgcacaccct
     7561 ccagccccag cgtattgatc gctttggtcg aagaaaacaa tgcggacagg gaaaaatcca
     7621 tccgcgccga tttcatccgg atctgcttgg tctcgccgat cgacacgtca ctcaactcca
     7681 gacgcggcgt tggcaacaat ctgccggaca tcttgccaat atggacaggt tgcttgaact
     7741 gcgtggccag catctgctcc agcttggcgg cgcgctcctg catcggcaat accagtggca
     7801 cgacaaacac ggcgaccacc atcagcacgc acagtcccac agcgaccttg ccccatggaa
     7861 tgggcttgcg tctggcttta actttcggca cttcgaccgg gggaacaggt tgtgccgcct
     7921 gggcggcccg ctcttcgctc gccttggctg cgcgctgctc cgcctccgac cacaccttgg
     7981 cctgtgccgc cgcgatcgtc gcaacctctt cctcggtcgg catatgcgcc ttcgcaacgg
     8041 gcttcggctc ctccttggca gggggcgcct gctctacctt ggtcggagcg ggcggcacct
     8101 gtccagaatt ggtttcgggc agtttgctct ccgcgccggc catggcatcg ccgagcaacg
     8161 tgctggtctg cgtatgctcc tcttcgggcg tcaccggctg aatcggggca gttccgattt
     8221 cgggggcggc gaagacgaag ggttccagct tgataggggc ggtcaccggc tggctgacag
     8281 gagcgatcac atcggcctct gaaatatcca ccagttcata gacggcatgt tcgcgtccat
     8341 gtttgtcctg ttttatcccg cgcgactgga acaggttggc gtactcatcg ctcagtctcg
     8401 agatgaagtc atagtagggg cgggaaatga atatctgatc taccccggca aaactcatca
     8461 cgcgttgcgc gtcgttgatg ccgtcgccaa ccatgttgct ctgaccgttc atgtccttga
     8521 cgatattgat cggtcccaga tgaatgccgg ttcgtacatt cagctccggg taatccgtgt
     8581 gccggtgggc cgtcaccgcc tcccggaact ggcgtgccac ctgcagcgca tcctcggggt
     8641 gatgcagaaa tccgattgcg gccccgtccc ccgtatccag aatgatgcgt tcggcctctt
     8701 ccagcgtccc cagacaatcc gagaccagct gattgaactg cttcttgatc ttgatctgct
     8761 tgttgaccgg ctgcttggta tagccgacca catcgaagaa caataccgtc acgctcacgc
     8821 tgctcgcccg cttcgcggtt ccaccctcga atttccgggc aggcgcgttc gcctccgctt
     8881 cctgctccgc cttgacgcgc aacgcttctg cttcctgctg agccttgatg cgtaccgcct
     8941 cggcctcttg ccgcgccttc agattggcgg cttctgtctc tgcgcgcatc ttcgctgcat
     9001 cttgttccgc cttgatgcgt tctgcctcgg ctcgcgcctt ctgttctgcc gcttctgccc
     9061 gaaccttctc cgcatcctgc tcagccttga ggcgtgcagc ctcggcttgc gccttctgtt
     9121 cggccgcttc tgcgcgcgcc ttctccgcgt cctcctcagc cttgatgcgt gcagcctcgg
     9181 cttcctgctg agccttcaat ttcgcggctt cgatttcctg acgcatcctg gcttccgcct
     9241 cgatcttcgc ctgaatctcc gccgcctgac gcttggccag ttccgcttct tcccgcgcct
     9301 gagccgcttc ttgttcggcc ttctgtcgcg cagcctcggc ctgttctctg gccttgtact
     9361 cggcctcttc tcgcgccttg gcctccgccg ccactttagc ctgggctgcg gctgcctgac
     9421 gctttgcctg ttcagcctcc tcgtgcgcct ttgccgccgc ccgctctgcc ttgaggcgag
     9481 ccgcttccgc ctcctcctga gctttcagct tcgcgatctc aatttccttg cgggcacggg
     9541 cttccgcctc ggcatgcgca cgcgcatctg cttcagcctg cgccttcgca gccgccaact
     9601 cggcacgaac ctgtgcggcc tcctgctccg ccttcaaacg cgcggcttct gcctgctccc
     9661 gggcttgatg ttccgcctgt tcacgcgcac gcgcctcagt tgcagccttc cgcttggcag
     9721 cctccgcttc ctcacgcgcc ttggcggctt cctgctcggc cttgaggcgt gcggcctcgg
     9781 catcctgctg cgccttcatc ttcaccagtt cggcttcctt gcgcaccttg gccgcctcct
     9841 gctctgccct gatgcgcgcc gcttcggctt cttgctgcgc tttctgttta gccgcctcga
     9901 tttctttgcg cgcattggct tcagcctctt cgcgcgccct tatttcaatc tcctttttca
     9961 gtctttcggc atcggccttg gccgcctcgg cagccaatac ggccggagaa gggacacggt
    10021 atgctgccgt gaaatccagc tcttcgagat tggtgctcac aggcttcttg gcaggaacca
    10081 ccatgcctgc cggaacagat agcttgggag catgaccaga tgcaggggca ttagcagact
    10141 tatgcgcctc atgaggcttg cccactttgg ccttgttctg gataaaacca gcactttcca
    10201 gctcgacgaa caacggctgc aacatctcgc gcatactcgg cgcggcacgc ttggaaagct
    10261 cgtccaaaga cgccttgcca tcgaccatta acagtgcacg cttaatatct ccggacagat
    10321 gcggcgttct gctgcgaatc tcgtcctcgc ccttgtctgt tttgacgaat atgcttttgt
    10381 tatccatgtt tcatctgact aataatcaat tcgattggtg atctgtatca cttggcctcc
    10441 cccttattgg ggtacgcctc cttggcatcg ttcttatcac gatgactgcc aaacaacttg
    10501 ttcaggaaac cgccgacctt gacgcccaat gccgtgccca gaccgggccc cagaatcgcg
    10561 gtccccatcg cggctccggc ccagacggag ctgctgaccc tgacgaccgg atcgttcaag
    10621 gtacccgaaa ccaccacggg catactcacc agtccagcgg tacctttgac atcaacatcc
    10681 agcacgccat tgagctgcaa tgtcggtgcg acatcaaccc ggccatcggc tttcaaggag
    10741 ccggacgata ttttgatcgc cctgaagtga taaccggaag cattcacgtt tatcgtccca
    10801 gtcaggtcgt caaatttggt cacgcccccc ttggcctcgg ccttactctg gctgctcacg
    10861 gcctgcacca gatcgacctt gctcaatacc ccgtcgcgca aatgaaaatt cccagtcagc
    10921 atcagattct ccgggaagcg gtcgagtctg tccagactca tcgaaaactt tgcctcgcta
    10981 tccaattttc cagaaaatag cacctcattg gtcagcgcct tcaccaacgg actcatattg
    11041 atttcatgca ccgagacctg cccattcagc atctcattgt cgtgcttgat actcatgccg
    11101 ccggtgagta caccgccata caaaacagcc gtcagtccgc tcatctccat ctgccgaggc
    11161 cccagcacga catgtgactg gatctgatcg aatgccaacg gctgcccgaa actctcggca
    11221 atgcgcgcct tgacattgac ggcatgcaaa tcgccaacga agctcaattc ttcggccagc
    11281 aatttccagt cactggcctt tccatccagc tttcccttgc cggacagcac accggacaac
    11341 ccgacaggct tgcccagcaa agccatcaat tgatccagtt gaatctcggc ttgattcacc
    11401 gaggcatgta tcttccatcc atttgaaaaa ttcagattac cggaacctgc cacatgcatg
    11461 ccacgcgagc taaccgagaa ttgctgcgca atgatcccct gcccggtaaa aacgccgtcc
    11521 agctgcaata tgtctagcgg caaccttacc ggcgggggcg acatccattc tttggctcgc
    11581 acctgaaatg cgatccccat attctgcaaa ggagtcagaa cggccgttaa ttttttctcg
    11641 tctaatgcga accacgcccg ctgcaacgcg cctgtgcggg tgaactcgat cttcccctcc
    11701 aatggcccga ccgccatctc cgggaaaagc aaaatcacgc cggacaacca catctcgccc
    11761 agccttggcc cctgctctac aagcggcgca tgcgacaaca cttccagcaa cttcctgaga
    11821 ggagcgaagt acgcggcccc atcccgcaac tcgacatgca tcaccaattg tccagccaac
    11881 aagtccgaca aattcggcga caccatgatc gaacggaccg caatcccttc ctgacctccg
    11941 attagcgcat cgcgcaactc gagatgaggc caggggaaaa tggccgcgcg caaactcccg
    12001 acgctgaccg gcatctgcaa tcgcgcactc agcgtttgct ccacctccgg cacatacaca
    12061 tcgagcggcg tcaggtatac cgtcagcatc gcgagcatca acaatgcgaa cactccgacc
    12121 agagcagact taaaccattt cataaaaagt tcaatctgct cttgacctgt ctggatgaaa
    12181 tcactatcat gcgcacctct tcattccctg atagctcagt cggtagagcg acggactgtt
    12241 aatccgcagg tccctggttc gagtccaggt cggggagcca aatagaataa gccttgcagc
    12301 aatgcagggc tttttcgttt ccaactgttt tttatcgcta ttctcaccac attcacgctg
    12361 tatttttgat ggcgacaccg caccattttt atgaaagagg tgcattcaaa aaacgctttc
    12421 gccaagatca aggatgttca ccactcaatt tattgtgtac tgcctaagct gataccgaac
    12481 gagatatgaa acttcatgac ccgttcaaag cggcggcaga catatggcag acattgtaat
    12541 gcttgggaca ctatatatgg gagaccccag aggggtgatg caaggaacgg cgaagattca
    12601 ctcatccgta tcgcagccat cccaaagatt tcccatgaaa cgacgataca acattgaaaa
    12661 gagggcttca tgctgaaagt atttctcacc atagacccgc agacactgcg cggactctac
    12721 gacgacgaag cgaaattgct cggaatcttc cagtccatga gcgaagccga agatgcactc
    12781 ggcgatatgc tcaacttctg caccatccac tccgaaagcc ccaatcagta gcacccaaag
    12841 tgccttcccc gctgtggacg acaccgttcc catcccgccc cgaccgggga gctcaggcgg
    12901 ccatggtgac cgccactcat tgcagaaaat aaactaaagc tttccgactt tctgccgaaa
    12961 atgctgtcag ttcatattta attcgcagga cagagaggat ggccagcatg cttcaaaata
    13021 caagtcatga catcgaacgc cagttagcgg cttgccttac tagcgacgcc accatgggga
    13081 gccgccacaa aacggcaagt aacgaagaag aggccagaga caggatgatt gcggcccgac
    13141 gggaacaaca acgtctcact atcctgcgta gattaaccac atccgggatg ttgtgtcctg
    13201 cgtgaatggc cgatgtggtt gctgattttt tccgcactca tagccgccgc acaaccggca
    13261 atgcgcattc aacggcaatc attcatgaca gctaaaaata cagcccctat tccatgcaac
    13321 aatttcaact aaacggcgag tttatcgagc ttaaccaact gctcaagatg gtcggcctat
    13381 gcgacagcgg cggcgcgggc aaagcgctgg tagcagaagg ccttgtttcc gtcgatggca
    13441 gagtggagtt gcggaaaacg gccaagatac gcgcgggctc agtggtatcg ctgggggacg
    13501 tgagcatcag cgtgcttgcc gcgtagctgc ataacggcag agttggccgc cttcatgtcc
    13561 tgcacaagcc tgcagaactt ccccacaatt accgtggatg ctcctgtgga gcagatgtgg
    13621 ataaccagaa aataacctgc cgagacagcc ctgtacaacg ggcgattaaa ggttgaactc
    13681 gccccctgtg ccggaaccat ccattggcag ggaatatcag cctcgggact cacgctagtg
    13741 gcagaaccgt ggatgcacta cactctccat gcaagcgcag taacctttaa cgtatttcaa
    13801 cccgcgatag attcagttca tcccgctctc atcactgaca ggcgaaaaac atcatgaaat
    13861 atcggcacta caagggtggc atctacgaaa tagtttgcga ggccagactc gaggcagatc
    13921 ccgatgtcat catggtcgtt tacaaatctg aagacggctt gatctggacg cgcccccgcg
    13981 acgttttctt tgagctggtg cagcacgaag gaaataccgt acagcggttc gccccgatta
    14041 attaaggatg catggtcatt ggccgactcc cgatccgccg catcaaggac tgagcttacc
    14101 ccactgagca tccagcacgc ggtcgcgccg gtcatccggc acgcgccgca taaacggaca
    14161 atcgtgccgg tggatggtga taccccggtc gcgggtgaca taaccgacga tggcatccgg
    14221 cgatgacggc gtacagcact tcgccaggcg tatcatcaga ttccccactc ccgccaccag
    14281 caccccctcc ggcgaagcct gacgaacgac gcttctgacg ggtgcagcat gctgcaccgc
    14341 ctctgcagac cgggatggca tctcttgctg aatggcaagg gatgcgcgcc gcaacgtgac
    14401 cttgccgcgc ccgagggcga tcagcaactc ctctgtcttg ttgaagcgca agcgctgcgc
    14461 aattttttcc tgattgatgc cgaccgcccc cagccgctgc aactcgcgct ccagcagcgc
    14521 ccttccctgc gcgacgctgt catccagccc ctgctgacgg aaccagtggc gcaccttcgc
    14581 ccgcgcgctt gaggtctgca aatagccgag cgcggggcta agccagtcgc ggctgggtgc
    14641 gcccagcttg gtggtcaata tctccacacg ctgaccgttc tgcagcgggt aaccgagcgg
    14701 gacgatgctg ccgttcacct tggcaccgcg cgtgcggtgc cccagatcgg tatgcaaggc
    14761 gtaggcgaaa tccaccggcg tcgccccttt gggcagatcg atgatacggc cctgcggcgt
    14821 gagcacatag acccggtcct gtatcagccc ctccctgaac tggccggaaa gttcggccag
    14881 ctcggacttc catttcagaa tctggcgcag ccaggcgatc ttctcgtcga agcccgcatc
    14941 agacttgccg ccttccttgt agcgccaatg cgcggcgacc cccagctcgg aatgctggtg
    15001 catctcgtgc gtgcgtatct gcacctcgac attcaacccg cgagggccga ccacggcggt
    15061 gtgcagcgaa cggtagtcgt tgctcttggg gtgggcgatg taatcgtcga actcgccggg
    15121 cacaggctgc cacaggtcgt gcaccagtcc gagcaccgca tagcagctct cgacatcgtc
    15181 gaccagcaca cgcaccgcgc gcacatcgta gagttcgtcg aaatccagct gtttgcgctt
    15241 catcttgttg acgatgctgt agatatgctt gggacgcccg ctgacctcgg cgcgaatatc
    15301 ggcccgcgcc aactcccgct ccagcagggc cagcagttcg gcgatatagc gctcgcgatc
    15361 cacgcgacgc tcgtccagca gcttggcgac ccgcttataa agctccggct ccagataacg
    15421 caccgaaaga tcttccagct cccacttgag ttgccatacc cccagccggt tcgccagcgg
    15481 cgcaaagatg cgctgcgtct cctgcgcgat tcctcgccgc agttgttcgt ctgcaccgga
    15541 caattcacgc agggtctggg tgcgctcggc cagcttgatc agcaccacac ggatgtcctg
    15601 caccatcgcc agcagcatct tgcgcaggct ctccagctgc cgtgccgcat cggccttgcc
    15661 gatcccctgc cctgcgccca gctgacggat ctgctccatg cgtgcgacgc cttcgaccag
    15721 tccgagtacc ggcgcggcaa aatcggcctc cagcttctcc ttccactcgt ccagacagtc
    15781 cggcaccgca tgcagcagag cggcggcaat cgcctcggca tccatattca ggtgaaccag
    15841 aatcgcggcg ctccccaatg catgcggcaa caatggcgtg ccggacgggc cggctttccc
    15901 ctcatacagc gatgcggcgc gctcataggc gcgacggatc agcgccgtct cgtccgcaga
    15961 aaagtgcgcc gccacggcat ccagccagga atctgcatcc tgatggttgg caagtgcgga
    16021 atggctatgc tgaacggaga ccatgaatat ggatgcgctg accgctacaa agacaccgtt
    16081 ccggcatcga gtcttgcgat gaaactctgc aaatccggcg gcagcggcgc ttcgatctgc
    16141 aacggttcgc cggtgagcgg atgattgaac gaaatcgaat gcgcgtgcag gaacatgcgc
    16201 ttcagcccct gcttcatcaa ggcccggttg cgcgcaaagt caccgtactt gtcgtcaccg
    16261 gcgataggaa accccagatg ggacagatgc acgcggatct ggtgggtgcg ccccgtcttg
    16321 agctcggctt cgagcaagct gaattcgtcc cagctctgtt gcaggctgaa gatggtatgg
    16381 gcctgctgac cgtcctcgcg caccatcacc cgcttctcgc cctggggggt atcgaacttg
    16441 tgcaacggca gtttgacgtg ctggcgcgcg ttcttccatt gccccagcac cagcgcaaaa
    16501 tagcgtttgt cgctgttgcc ctcgcgcatg atctcgtgca ttccggtcag cgccgtacgc
    16561 ttcttcgcga ccagcagcac accggaagtc tctcgatcca gacgatgcac cagttccagc
    16621 agcttggcct gagggcgcgc gcggcgcaat tgttcgatga cgccgaagct caccccgctg
    16681 ccgccatgca ccgccacccc ggagggcttg ttgatcgcca gcagcgcgtc gtcctcgtac
    16741 aggatgggga attccgccgc cggcacgaat tcggcctccg ccttttccgc gacccggatc
    16801 ggcggaatgc gcagcaggtc gcccaattgc agacgatagg tctggggcac gcgcttcttg
    16861 ttcacccgca cctcgcccgc acgcaagatg cggtagacat ggctcttcgg cacgcctttc
    16921 aggtgacgga acaggaagtt gtcgatgcgc t
//
LOCUS       DASUIV010000043        15043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_753087, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000043 DASUIV010000000
VERSION     DASUIV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 15043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15043
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753087"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..575)
                     /gene="grpE"
                     /locus_tag="VFW59_01430"
     CDS             complement(21..575)
                     /gene="grpE"
                     /locus_tag="VFW59_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5362908.1"
                     /translation="MEQKETNPQIEQQQVAEGVEAAAEGTTETMPSLEEMLQAAERKA
                     QEHYDAWMYAKAEGENIRRRAADDVSKAQKFAVERFSGEMLAVMDSLQAGLAVQTENV
                     ESFKSGMELTLKQLSSVFEKFHISEINPMGEKLDPHKHQAIGMLESDEPANTVVSVMQ
                     KGYSLNERVLRPAMVMVAKAKDPG"
     gene            692..1855
                     /locus_tag="VFW59_01435"
     CDS             692..1855
                     /locus_tag="VFW59_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5362909.1"
                     /translation="MPTTKNYHWRIAGFYFFYYAFVGMFAPYWSLYLQSLHFSVIEIG
                     ILMSMQPVMRMLAPNVWGWLADHTGRRLTVIKIAATLSALFYLGVFLTTSFWGMMLVL
                     VLMNFFWSASMPLVEATTLSYLGKHTAHYGRIRSWGSVGFIVAVVGLGYAFDYIAIAW
                     LLWAGLVCEIGILLFARQIPPTEVVAHHTDNQSIRQVVLQPRVLALFGACFMMSVAHG
                     PYYTFYSIYLVEHGYAKSAVGGLWALGVICEIGVFFLMPALVRRFGYVHILLASFAAA
                     VVRFMMIGWAVDVVVLLLLAQVLHALTFGSYHAASVGLVHEFFRGRHQSKGQALFGSV
                     TYGAGGMLGSLASGPIWQHYGASMLYSFSALMGVLGLLLMAWMLGMDGERRPG"
     gene            1928..2161
                     /gene="hfq"
                     /locus_tag="VFW59_01440"
     CDS             1928..2161
                     /gene="hfq"
                     /locus_tag="VFW59_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX5362910.1"
                     /translation="MSSKGQQLQDPFLNTLRKEHVQVAIYLVNGIKLQGHIDSFDQYV
                     VLLKNTVTQMVYKHAISTIVPARAVNIVYDAAE"
     gene            2161..3351
                     /gene="hflX"
                     /locus_tag="VFW59_01445"
     CDS             2161..3351
                     /gene="hflX"
                     /locus_tag="VFW59_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5362911.1"
                     /translation="MQHQSAGSEIAVLVSLDLGEPDYAESLEELRLLAESAGVKTLAI
                     IEGKRQRPDSALFAGSGKVQEVADLLAAHEEVGVVIFNHDLSPAQMRNLTAKLERRVI
                     DRTMLILDIFAQRAQSHEGKVQVELAQLRYLATRLVGMNMDMGQQKFAVGARGPGETQ
                     LELDRRKLDKRVHLLKDRLAKLKRHRQVQRKSRNRGDVLSVSIVGYTNAGKSTLFNAL
                     THAKVYAADQLFATLDTTTRRIYLAAADDGIAAEDVDHTQAYAAPGQVVLSDTVGFIR
                     HLPHSLVAAFRSTLEETIQADLLLHVVDANSANRDAQIAEVNKVLEEIDAQHIPQILV
                     LNKIDLLDVQPGIERDEYGRIDTIRLSARTGAGLDDLRAALVEIRDAEPVEAPAQEWH
                     PLNN"
     gene            3363..4550
                     /gene="hflK"
                     /locus_tag="VFW59_01450"
     CDS             3363..4550
                     /gene="hflK"
                     /locus_tag="VFW59_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX5362912.1"
                     /translation="MGLNDPQWGNKNSGGGNGGPPDLEELLRKLNEKVAALMGGKKRD
                     SSGGSAPSQGFGGSIGLILIIVALVWVGSGFYIVNEGQRGVVLRFGKFVEATQPGPRW
                     HLPFPVEAVEVVNLSQVRTVEVGYRENVKNKMLKESLMLTDDENIVDIQFAVQYFLKD
                     PSDYLFNNRMPDENVRQAAETAIREVVGKSKMDFVLYEGREQVAAGATKLMQEILDRY
                     KSGIVISKLTMQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQAYANDVIPRASGAAAR
                     LLQEAEGYKQSVIANAEGDASRFKQILVEYEKAPGVTRERMFLDMQQQVLTSVSKVLV
                     DQKNGSSLLYLPLDKLIESTKAAGDATGVPVVAPAPTAQSSDNNASQRARDSLLSRDR
                     EAR"
     gene            4551..5429
                     /gene="hflC"
                     /locus_tag="VFW59_01455"
     CDS             4551..5429
                     /gene="hflC"
                     /locus_tag="VFW59_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX5362913.1"
                     /translation="MDKRVSNILVGAVGALILLGLSVFVVDQRQNAIVFQLGEVVSVK
                     TEPGLYFKVPLMQNVRYFDSRILTLDTVDPERFITAEKKNVLVDSYVKWRIVDVKQYY
                     VSVGGDEVRANTRLLQTVNSSMREEFGKRTIHEVVSGERDKIMEVLRTKADADARKIG
                     VEVLDVRLKRVDFPAEISGSVYSRMDAERKRVANELRASGAAEGEKIKADADKQREVI
                     LAEAYREAQKTKGEGDAKASALYAAAFGRNPEFYSFYRSLEAYKQSFKSKSDVMVLDP
                     SSAFFKYLKSSGKSGK"
     gene            5445..5630
                     /locus_tag="VFW59_01460"
     CDS             5445..5630
                     /locus_tag="VFW59_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX5362914.1"
                     /translation="MLDFWLMALGLMLVLEGMMPFLFPSAWRETLRRLVQFQDGQLRF
                     LGLTLMLSGLLLIYWIK"
     gene            5631..6791
                     /locus_tag="VFW59_01465"
     CDS             5631..6791
                     /locus_tag="VFW59_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX5362915.1"
                     /translation="MPNWLLPEYIQDMLPEEAWRVEAMRAQFLELMRKNGYQLVAPPL
                     LEYAESLLLDGSEDMDLRSFKLVDQLSGRTLALRADITPQVARVDAHLLNRQGVARLC
                     YAGSVLHTQPVGLTRTRELLQIGAELYGHSGPESDLEIQQLMLQTLAALGIEHVHLDL
                     GHVGVFRALVRHAGLDKRLENQLFAALQSKDSATLQELVQPLDTVVREALLVLPTLYG
                     DCDQVLARARRSLPALPEIAAALDALQVTCAKLQPLVARVGIDLADLRGYHYHSGMVF
                     AAYHEGSHDAIALGGRYDDLGKSFGRARAATGFSMDLRQLYGLLSPQALPPGICAPYF
                     DDAALRDKIAQLRAAGEAVVVDLLGDAALRRELQCDRELVSRDGVWQVVALG"
     gene            6935..8245
                     /locus_tag="VFW59_01470"
     CDS             6935..8245
                     /locus_tag="VFW59_01470"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5362916.1"
                     /translation="MAKNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTL
                     VIGGKKTVLHLIPSGILRDHVMCYIGNGVVLSPQALLKEMDELQKAGIDVYSRLKVSE
                     ACPLILPYHEAIDKARELAKGDAKIGTTGRGIGPAYEDKVARRAIRLQDIFFRERFAA
                     KLGEVLDYHNFVLKNYFQAETVDFQKVLDETLALGERIKPLVMDVPRALYDANQADNN
                     LLFEGAQGALLDIDHGTYPYVTSSNCISGAACAGAGVGPQMLNYVLGITKAYTTRVGS
                     GPFPTELYDAVDKRDPIGEGLARRGHEFGSTTGRARRCGWFDAAALKRSIQINGVSGL
                     CITKLDVMDGMEVVRLGVGYRINGVESDILPAGADALVDCQPVYEEMPGWSESTVGIR
                     RYEDLPQAARNYLERIALVCGVPVDMVSTGPDRDETIVLRHPFA"
     gene            complement(8406..8490)
                     /locus_tag="VFW59_01475"
     tRNA            complement(8406..8490)
                     /locus_tag="VFW59_01475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8454..8456),aa:Leu,seq:cag)
     gene            8534..10792
                     /gene="rnr"
                     /locus_tag="VFW59_01480"
     CDS             8534..10792
                     /gene="rnr"
                     /locus_tag="VFW59_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX5362917.1"
                     /translation="MKKKNNLRLQDPFLDREREQYEHPLPSREYILQILADQGGPVDQ
                     DELLHLLHIELHEEDLFSRRLRAMERDGQIMRNRKRAICVADKLDLVKGKVQGHPDGF
                     GFLVPEDGSPDMFLSEKEMHKVLHGDVVMARQSGVDRRGRPEGKIIEVLERGNSRVVG
                     RLHEEHGIWFVVAENQRISQDILVAQGETGGAKAGQVVILEIIKHPDKHAQPIGRIVQ
                     VLGNYADPGMEIEIALRKHDLPYEFPHDVEKQAKAIPPVVVAEDWAGREDIRNLPLVT
                     IDGETARDFDDAVYCAPEKGGFRLVVAIADVSHYVQTNDALDREAILRGNSVYFPRRV
                     IPMLPEELSNGLCSLNPHVDRMCMVCDMHVGNDGEIGKYRFYPSVMYSHARLTYTQVA
                     EMLGQPDGELAKSNADILPHLQHLYELFQKLLHAREKRGAIDFETVETQMIFNDQGKI
                     ERIVPVVRNDAHKLIEECMLAANVCAADYLHEHKHTVLYRIHQGPTPEKLVALREFFK
                     EFGLDLGGGDEPQASDYSKLLKKIKGRPDAGLLQTVMLRSLRQAKYEPENIGHFGLGY
                     EAYTHFTSPIRRYPDLLVHRAIKAVLHGKKYTPGMKWEELGAHCSMTERRADDATRDV
                     EAWLKCFYMRDHLGSVFTGTVSSVTGFGMFVSLDDLYVEGLVHISELGKDYYHFDAAK
                     HQLLGERTGNRYRLGDRVQVRVVKVDMESTKVDFVLHDAAPAVESEDEPKKSDAKKSG
                     PKKSSGGKKKHG"
     gene            10785..11528
                     /gene="rlmB"
                     /locus_tag="VFW59_01485"
     CDS             10785..11528
                     /gene="rlmB"
                     /locus_tag="VFW59_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5362918.1"
                     /translation="MADLRLIYGFHAVTSRIRQNPDGVQEIYLQAQRQDPRMRDLIKL
                     AETNGVRIIPVDAKRLDGMAGNERHQGVAARVDAAQRVQHLDDVLDTLTEPPLLLVLD
                     GVQDPHNLGACLRSADAFGVHAVIAPKDRAVGINATVEKVACGAAETVPFITVTNLAR
                     TLRELQERDIWVVGAAGEAEQDLHGFKHKGGLAWVLGAEGEGLRRLTRETCDELVRIP
                     MLGSVESLNVSVSAGICLFEARRQRQGGV"
     gene            11630..12025
                     /locus_tag="VFW59_01490"
     CDS             11630..12025
                     /locus_tag="VFW59_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362919.1"
                     /translation="MKKKKKSLRVPVTRGLKDIYAMDMHTAYQAACMGQFNVDAFGRL
                     AAAISVIRSALEQNRTRIPDAVETMDAAIATLLAVRARGDETGRWEIAEAERPSVLAG
                     IGMAEQSIGTLDVALLAQTADDLLASISG"
     gene            complement(12022..12588)
                     /gene="amrA"
                     /locus_tag="VFW59_01495"
     CDS             complement(12022..12588)
                     /gene="amrA"
                     /locus_tag="VFW59_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX5362920.1"
                     /translation="MSDPAFDQRRGKILLDIARGSIAEALGAPHLAVPQEDWLQQPGA
                     TFVTLTQHGELRGCIGTLEAHRPLAEDVYHNARAAAFEDPRFMPLKREELGYTRVEVS
                     LLSPPQPMTVQDEADALAQLRPGVDGLVFEYGRYRSTFLPQVWEQLPLPRQFMAHLKR
                     KAGLPEDFWSDDVRLSRYTVTKWSEPRA"
     gene            complement(12581..13381)
                     /gene="amrB"
                     /locus_tag="VFW59_01500"
     CDS             complement(12581..13381)
                     /gene="amrB"
                     /locus_tag="VFW59_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX5362921.1"
                     /translation="MSSVRPPAVAGIFYPADPRQLAHDLGELLNEATPHDLIPKALIA
                     PHAGYIYSGAIAASAYATLLPVADRIRRVILLGPTHRVAIRGLALPGTDAFDTPLGRI
                     TVDAAAARAIADLPQVSISPLVHAQEHSLEVHLPFLQSVLKDFTLLPLAVGMATAEEV
                     AEVLERLWGGEETLIVISSDLSHYLPYTTARHVDKQTVQSMLQLQQPLSHEQACGATP
                     INGLILAAHRHRLTPHLLDLRNSGDTAGSRDQVVGYAALAFTAGDRDE"
     gene            13515..14597
                     /locus_tag="VFW59_01505"
     CDS             13515..14597
                     /locus_tag="VFW59_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5362922.1"
                     /translation="MNTVQLSVVIPVYNEEQGLQALFGRLYPALDKLGIRYEVVFVND
                     GSRDRSAAILREQFQLRPDVTRVVLFNGNFGQHMAIMAGFEHSRGQRVVTLDADLQNP
                     PEDIGVLLAKMDEGHDYVGSIRRQRNDSAWRHYASRAMNRLRERITRIKMTDQGCMFR
                     AYDRNIIDAVNSCHEVNTFIPALAYTFARNPAEVVVGHEERAAGESKYSLYSLIRLNF
                     DLMTGFSLVPLQWFSMAGMLISLFSGLFFVLLVLRRLVIGPEAEGLFTLFALMFFMIG
                     IALFGIGLLGEYVGRIYQQVRHRPRYLVEAVLEIEDRGSRIAERGLGSAEKTAVAGEP
                     DVAEPRKRKAKPVEPVHPSLFSDTEE"
     gene            14594..>15043
                     /locus_tag="VFW59_01510"
     CDS             14594..>15043
                     /locus_tag="VFW59_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX5362923.1"
                     /translation="MSRAVVFAYHNVGYRCLSVLLAQGVDVALVVTHRDNPKETIWFE
                     SVQKLAELHGIPTITPDNPNTPDVTEQIAALQPDFFFSFYYREMLKRELLEIPKRGAL
                     NMHGSLLPKYRGRVPVNWAIICGETETGATLHYMTEKPDNGDIVAQQA"
BASE COUNT         2792 a         4280 c         5011 g         2960 t
ORIGIN      
        1 attcttttgg ttcgggtcgt ttagcccggg tctttcgcct ttgcaaccat caccatggcg
       61 gggcgcagca cgcgttcatt caggctgtaa cccttctgca tcacgctgac cacggtgttg
      121 gcgggttcgt cgctttccag catgccgatg gcctgatgct tgtgcggatc cagcttctcg
      181 cccatcgggt tgatctcgct gatgtggaac ttctcgaaca cgctggaaag ctgcttcagc
      241 gtcagttcca tgccgctctt gaagctctcc acattctcgg tctgtaccgc aagaccggcc
      301 tgcaggctgt ccatcaccgc cagcatttcg ccggaaaagc gctcgacggc gaatttctgt
      361 gccttgctca catcgtcggc ggcgcggcgg cggatgttct cgccctcggc cttggcatac
      421 atccaggcat cgtaatgttc ctgcgccttg cgctcggcag cctgcaacat ctcctccagg
      481 ctgggcatgg tctcggtcgt accctcggct gcggcctcaa cgccctcggc gacctgttgt
      541 tgttcgattt gcggattggt ttctttctgt tccatgcgac tctcctgtta gcgacccgtg
      601 cttagttggg gcgaggcgca gcttttcaag agcaatcgca aaatattttt tgcccatgag
      661 ataatgcgcg ccgtccagaa gcggctcttt catgcctacc accaagaact atcactggcg
      721 catcgccgga ttctattttt tctactacgc cttcgtgggg atgttcgcgc cctactggag
      781 tctgtatctg caatcgctgc atttttccgt catcgagatc ggcatcctga tgtcgatgca
      841 gccggtgatg cgcatgctgg cacccaatgt ctggggttgg ctggcggatc ataccgggcg
      901 gcgcctgacg gtgatcaaga tcgcggcgac gctgagtgcg ctgttctatc tgggtgtgtt
      961 cttgaccacc agcttctggg ggatgatgct ggtgctggtg ctgatgaact tcttctggag
     1021 cgcctccatg ccgctggtcg aggcgaccac gctgagctat ctgggcaagc acaccgcgca
     1081 ctacgggcgc atccgttcct gggggtcggt cggcttcatc gtcgcggtgg tggggttggg
     1141 ctatgccttc gattacatcg cgatcgcctg gctgctctgg gccgggctgg tgtgcgagat
     1201 cggcattctg ttgtttgcgc gccagattcc gcccaccgag gtggtggcgc atcacaccga
     1261 caatcagtct atccgccagg tcgtgttgca gccgagagtg ctggcgctgt tcggcgcctg
     1321 cttcatgatg tcggtcgcac acgggcctta ctacaccttt tattccatct atctggtcga
     1381 gcacggctac gccaagagcg cggtgggcgg tttgtgggcg ctgggggtga tctgcgagat
     1441 cggtgtgttc ttcctgatgc ccgcgctggt gcggcgtttc ggctatgtgc atatcctgct
     1501 ggccagcttc gcggcggcgg tggtgcgctt catgatgatc ggctgggccg tggatgtggt
     1561 ggtgctgttg ctgctggcgc aggtgctgca cgcgctgacc ttcggctcct accatgccgc
     1621 atcggtgggg ctggtgcatg agttcttccg tggtcgccac cagtccaagg ggcaggcgtt
     1681 gttcggcagt gtgacttacg gggccggcgg catgctgggc agtctggcga gcggtccgat
     1741 ctggcagcat tacggcgcca gcatgctgta ttcgttcagc gcgctgatgg gcgtgttggg
     1801 gttgctgctg atggcctgga tgctggggat ggacggtgag cggcggccgg ggtgaagtga
     1861 ggcggttcac agccgccccg gaatccagta aagtggctac ccgttcctaa ccgataagag
     1921 caataacatg agttcaaaag gacaacaact gcaagatccg tttctgaata cgctgcgcaa
     1981 ggagcatgtt caggtggcca tctatctggt gaatggcatc aagctgcaag gccatatcga
     2041 ctcgttcgat cagtatgtcg tgctgctgaa gaacacggtc acgcaaatgg tttacaagca
     2101 tgccatttcc accatcgttc cggcacgcgc ggtcaacatc gtttacgacg ctgcagagta
     2161 atgcagcatc aatccgctgg ttctgaaatc gccgtattag tcagcctcga tctgggcgaa
     2221 ccggattatg ccgagagcct ggaagagttg cgcctgctgg ccgagtccgc aggtgtgaag
     2281 acgctggcga tcatagaggg taaacgtcag cgtcccgatt ctgcattgtt tgccggtagc
     2341 ggcaaggtgc aggaggtcgc ggatctgctc gccgcgcatg aagaggtcgg ggtcgtcatc
     2401 ttcaatcacg atctgtctcc cgcgcagatg cgcaacctca ccgccaagct ggagcggcgc
     2461 gtgatcgacc gcaccatgct gattctggac atcttcgcgc aacgcgcgca aagccacgag
     2521 ggcaaggtgc aggtggagct ggcacagctc aggtacctcg ccacccgtct ggtcggcatg
     2581 aatatggaca tggggcagca aaaattcgcg gtgggcgcgc gcggtcccgg cgagacccag
     2641 ctggagctgg acaggcgcaa gctggacaag cgcgtccatc tgttgaagga caggctggcc
     2701 aagttgaagc gccatcgtca ggtgcagcgc aaatcgcgca atcgcggcga tgtgctgtcg
     2761 gtatccatcg tcggctatac caacgcggga aagtcgacgc tgttcaatgc gcttacccac
     2821 gccaaagtct atgcggcaga tcagctgttc gccacgctgg acacgaccac aaggcgcatc
     2881 tatctggcgg cagcggatga cgggattgcg gctgaagatg tcgatcacac gcaggcctat
     2941 gccgcgccgg ggcaggtcgt gctgtccgat accgtcggct tcatccgtca cctgccgcat
     3001 tcgctggttg ccgcgttccg cagcacgctg gaagagacga tacaggccga tctgttgctg
     3061 catgtggtgg atgcgaacag cgccaatcgc gacgcccaga tcgccgaggt gaacaaggtg
     3121 ctggaggaga tcgacgcgca gcatattccg cagattctgg tgctcaacaa gatcgatttg
     3181 ctggacgtgc agccgggtat cgagcgggac gagtatggta gaatcgatac cattcgcttg
     3241 agtgccagaa cgggcgcggg attggacgat ttgcgcgccg cgctggtgga aattcgtgat
     3301 gccgagccgg tcgaagcgcc ggcgcaggaa tggcatccgc taaataattg attgggtgag
     3361 ttatgggact gaatgacccg caatggggta acaagaatag cggcggcggc aacggcggac
     3421 cgcccgacct ggaagagttg ctgcgcaagc tcaacgaaaa ggtcgctgcc ttgatgggtg
     3481 gcaagaagcg ggactcctcg ggcggtagcg caccgagtca ggggttcggc ggcagcatcg
     3541 gtctgatcct gatcatcgtc gcgctggtat gggtgggcag cggcttctat atcgtcaacg
     3601 aggggcagcg cggcgtggtg ttgcgcttcg gcaagtttgt cgaggcgacc cagccgggcc
     3661 cgcgttggca tctgccgttc ccggtcgagg ccgtggaagt ggtcaacctc agtcaggttc
     3721 gtaccgtgga agtcggctat cgcgagaacg tcaagaacaa gatgctgaaa gagtcgctga
     3781 tgctgaccga cgacgagaat atcgtcgaca tccagtttgc ggtgcagtac ttcctgaaag
     3841 accccagcga ctatctgttc aataaccgca tgccggacga gaacgtgcgt caggctgcgg
     3901 aaaccgcgat tcgcgaagtg gtcggcaaga gcaagatgga cttcgtgctg tacgaggggc
     3961 gcgagcaggt ggcggctggc gcgaccaagc tgatgcagga gattctggat cgttacaagt
     4021 ccggtatcgt gatcagcaag ctgaccatgc agaacgccca gccgccggaa caggtgcagg
     4081 ccgcgttcga cgatgcggtg aaggcgggtc aggatcgcga gcgccagaag aacgaaggcc
     4141 aggcttatgc gaacgatgtg attccgcgcg ccagcggcgc cgctgcgcgt ctgttgcagg
     4201 aagcggaagg ttacaagcag agcgtgatcg ccaatgccga gggtgatgcc agccgcttca
     4261 agcagattct ggtcgaatac gagaaggccc ccggtgtgac ccgcgagcgt atgttcctgg
     4321 acatgcagca gcaggtgttg accagcgtca gcaaggtgct ggtggaccag aagaacggca
     4381 gcagtctgct gtatctgccg ctggacaagc tgatcgagag taccaaggct gccggagatg
     4441 cgaccggcgt gcccgtcgtt gcgcctgcgc cgactgccca gtccagcgat aacaatgcat
     4501 cacagcgcgc gcgcgattcg ctgctgagcc gtgatcggga ggcccgctaa atggataaga
     4561 gagtcagtaa catcttggtc ggcgcggttg gtgcgctgat cctgttgggt ttgagcgtgt
     4621 ttgtggtgga tcagcgccag aatgcgatcg tgttccagct gggcgaagtg gtcagcgtca
     4681 agaccgagcc gggcttgtat ttcaaagtgc ccttgatgca gaacgtgcgc tatttcgatt
     4741 cgcgcattct gacgctggat acggtcgatc cggagcgttt catcaccgcc gagaagaaga
     4801 acgtgctggt ggattcctac gtcaagtggc gcatcgtgga cgtgaagcaa tactacgtca
     4861 gcgtgggcgg cgatgaagtg cgcgccaaca ctcgtttgtt gcagacggtc aattccagca
     4921 tgcgcgagga gttcggcaag cgcaccatcc acgaggtggt gtcgggcgag cgcgacaaga
     4981 tcatggaggt gttgcgcacc aaggcggatg ccgatgcgcg caagatcggc gtcgaagtgc
     5041 tggacgtgcg tttgaagcgc gtcgacttcc cggcggagat cagcggatcg gtctatagcc
     5101 gcatggatgc cgagcgcaag cgggtcgcca acgagttgcg cgcttccggt gcggccgagg
     5161 gcgagaagat caaggccgat gccgacaagc agcgcgaggt gattctggca gaagcctatc
     5221 gcgaagcgca gaagaccaag ggcgagggcg atgccaaggc ttccgcgtta tatgcggcgg
     5281 cattcggacg caatcccgag ttctactcct tctaccgcag tctggaagct tacaaacaga
     5341 gcttcaagag caagagcgac gtgatggtgc tggatccgag ttcggcgttc ttcaagtatc
     5401 tgaagagttc gggcaaatcg ggcaagtgat gcccgcatcg catcgtgctt gatttttggt
     5461 tgatggcgct gggtttgatg ctggtgctcg aaggcatgat gccgttcctg tttccctccg
     5521 cctggcgcga gactttgcgc aggctggttc agtttcagga cggccagttg cgctttcttg
     5581 gtctgacgct gatgctgagt ggcctgttgt tgatttactg gattaaataa atgccgaatt
     5641 ggttgttgcc ggaatacata caagacatgt tgcccgagga agcgtggcgc gtcgaagcga
     5701 tgcgtgcgca attcctcgaa ttgatgcgca agaacggcta tcagctggtc gctccgccgc
     5761 tgctggaata tgccgaatcg ctgctgctcg atggcagcga agacatggat ctgcgctcct
     5821 tcaaactggt tgaccagttg agcgggcgta cgctggcctt gcgtgcggac atcacgccgc
     5881 aggtggcgcg ggtggatgcg catctgctta accgtcaggg cgtggcgcgc ctgtgctatg
     5941 cgggcagcgt gctgcatacc cagccggtcg gattgacccg cacgcgcgag ttgttgcaga
     6001 tcggtgccga gttgtacggg cacagcggcc ccgaaagcga tctggagatt cagcagttga
     6061 tgctgcaaac tctggctgcg ctgggcatcg agcatgtgca tctcgatctg gggcatgtcg
     6121 gcgtgttccg cgcgctggtg cgtcacgccg gactcgacaa gcggcttgaa aatcagctgt
     6181 tcgccgcgct gcagagcaag gacagtgcga ccctgcagga actggtgcag ccgctggata
     6241 ccgtcgtgcg cgaggcgttg ctggtcctgc cgacgctgta cggcgattgt gatcaggtac
     6301 tggcgcgggc gcgccggtcg ctgccagcgc tgcccgagat cgccgccgcg ctggatgcgt
     6361 tgcaagtgac ctgcgccaaa ttgcagccgc tggtcgcacg cgtcggcatc gatctggccg
     6421 acctgcgcgg ttaccactat cacagcggca tggtgtttgc ggcctaccat gagggcagtc
     6481 acgatgccat cgcgctgggc gggcgctatg acgatctggg caagagcttc ggtcgcgcgc
     6541 gcgcggcgac cggcttcagc atggatctgc gccagttgta cggcctgctg tcgccgcaag
     6601 cgttgccgcc gggaatatgc gcgccttatt tcgacgatgc cgctttgcgc gacaaaatcg
     6661 cccagttgcg tgcggcgggc gaggcggtgg tggtggattt gctcggcgat gccgcattgc
     6721 gccgcgaact gcagtgcgat cgcgagctgg tgtcgcgtga cggtgtgtgg caagtcgtcg
     6781 cgttgggtta agactcgcag ggttattttt attctccttg ttcccgctgg cgggatcacc
     6841 agcgacttgg cggctttgca gccgggtcga atggctggtc gttttaccgg agggaagggg
     6901 agagggattc gattgataga cgggaagtat tgcaatggct aagaacgtag tagtaatcgg
     6961 tacccagtgg ggtgatgaag gcaagggcaa gatcgtcgac tggttgaccg atcatgcgca
     7021 gggcgtggtg cgctttcagg gcggtcacaa tgccgggcac acgctggtca tcggcggcaa
     7081 gaaaacggtg ctgcacctga tcccttccgg catcctgcgc gatcacgtga tgtgctacat
     7141 cggcaacggt gtggtgctgt ctccgcaggc gctgctcaag gaaatggacg agttgcagaa
     7201 ggccggcatc gatgtgtaca gccggttgaa ggtctccgag gcctgcccgt tgatcctgcc
     7261 ttaccatgag gcgatcgaca aggcgcgcga gctggccaag ggcgatgcca agatcggtac
     7321 caccggtcgc ggtatcggtc cggcctatga agacaaggtc gcgcgtcgcg ccatccgcct
     7381 gcaggacatc ttcttccgtg aacgcttcgc cgcgaagctg ggcgaggtgc tggattatca
     7441 caatttcgtg ctgaagaatt atttccaggc cgagacggtc gatttccaga aggttctgga
     7501 cgagacgctg gcgctgggcg aacgcatcaa gccgctggtg atggatgtgc cgcgcgcgct
     7561 gtatgacgcc aatcaggcgg ataacaatct gctgttcgag ggcgcccagg gcgcgttgct
     7621 ggacatcgat cacggcacct atccctacgt cacctcaagc aactgcatct cgggtgcggc
     7681 ctgcgcgggt gcaggggtgg ggccgcagat gctcaactac gtgctgggca tcaccaaggc
     7741 ttacaccacg cgcgtcggtt cggggccgtt cccaaccgag ctgtatgacg ccgtggacaa
     7801 gcgcgatccg atcggcgagg ggttggccag gcgcggccat gaattcggct ccacgaccgg
     7861 tcgtgcgcgc cgctgtggct ggttcgacgc ggcagcattg aagcgttcga tccagatcaa
     7921 cggggtgtcc ggcctgtgca tcaccaagct ggacgtgatg gacggcatgg aggtggtgcg
     7981 tctcggcgtc ggctaccgca tcaacggggt ggagagcgac atcctgccgg cgggcgccga
     8041 tgcactggtg gattgccagc cggtgtatga agaaatgccg ggctggagcg agagtaccgt
     8101 cgggatccgt cgttacgagg atctgcccca ggccgcgcgc aactatctgg agcgcatcgc
     8161 tctggtctgt ggcgtgccgg tggatatggt ttcgaccggt ccggaccgcg acgagaccat
     8221 cgtgctgcgt catccgttcg cttgagctac ccagtagctg ccgcgatcgc tgcgccgtgt
     8281 ctggttcttt gagtctttgt ggtatgaagt gtgtgtcacg catcgcgatg tctgcggtgc
     8341 gtgttttatt tttatgtgtt tcgcaagacg tgaaaaaggc ccgcattgct gcgagccttt
     8401 gtcgttggtg cccagaagag gactcgaacc tccacacctt gcggcacacg gacctgaacc
     8461 gtgcgcgtct accaattccg ccatctgggc ttgttgaagg cgcgcaattt aatagttaaa
     8521 ggacttgttg tcaatgaaaa agaaaaataa tttacgtttg caggatccat tcctcgatcg
     8581 cgagcgcgaa caatacgaac atccgctgcc tagccgcgag tacattttgc agattctggc
     8641 cgaccagggc ggcccggtag accaggatga gctgctgcat ctgctgcata tcgaactgca
     8701 cgaggaagac ctgttctccc gccgtctgcg cgcgatggag cgcgacggac agatcatgcg
     8761 caaccgcaaa cgggcgatct gcgtggcgga caagctcgat ctggtcaagg gcaaggtgca
     8821 gggccatccg gacggcttcg gcttcctggt gccggaagac ggcagcccgg acatgttcct
     8881 cagcgagaag gagatgcaca aggtgctgca cggcgacgtc gtgatggcaa gacaaagcgg
     8941 cgtcgaccgg cgcggccgcc ccgagggcaa gatcatcgag gtgctggagc gcggcaacag
     9001 ccgcgtggtg ggccgtctgc acgaggagca cggcatctgg ttcgtggtcg ccgagaacca
     9061 gcgcatcagc caggacatcc tggttgccca gggcgagacc ggcggcgcca aggccggtca
     9121 ggtggtcatc ctcgaaatca tcaagcatcc ggacaagcac gcgcagccga tcggccgcat
     9181 cgtgcaggtg ctgggcaatt atgccgatcc cggcatggag atcgagatcg cgctgcgcaa
     9241 gcatgacttg ccttacgagt ttccccatga cgtggagaag caggccaagg cgattcctcc
     9301 cgtggtcgtg gctgaagact gggccggacg cgaggacatc cgcaacctgc cgctggtcac
     9361 catcgacggc gagaccgcgc gcgacttcga cgatgcggtg tattgcgcgc cggagaaggg
     9421 cggtttccgt ctggtggtgg cgatcgccga cgtcagccat tatgtgcaga ccaacgatgc
     9481 gctggacagg gaagcgatcc tgcgcggcaa ctcggtgtac ttcccgcgcc gcgtgatccc
     9541 gatgctgccg gaggagctgt ccaacggcct gtgttcgctg aacccccatg tcgaccgcat
     9601 gtgtatggtg tgcgacatgc acgtcggcaa cgatggcgag atcggcaaat accggttcta
     9661 tccctcggtg atgtattcgc atgcgcgtct gacctacact caggtcgccg agatgctggg
     9721 gcagccggac ggcgaactgg ccaagagcaa tgccgacatc ctgccgcatc tgcaacacct
     9781 gtacgagctg ttccagaagc tgctgcatgc gcgcgagaaa cgcggcgcga tcgacttcga
     9841 aacggtcgag acgcagatga tcttcaacga tcagggcaag atcgagcgca tcgtgccggt
     9901 ggtgcgcaac gatgcgcaca agctgatcga ggagtgcatg ctggcggcca acgtgtgtgc
     9961 ggcggactat ctgcacgagc acaagcacac cgtgctgtac cgcatccatc agggcccgac
    10021 gccggagaaa ctggtggcgt tgcgcgagtt cttcaaggaa ttcggtctgg atctgggcgg
    10081 cggcgacgag ccgcaggcct ccgattactc caagctgctg aagaagatca agggtcgtcc
    10141 cgatgcgggc ttgttgcaga cggtgatgct gcgctcgctg cgtcaggcca agtatgagcc
    10201 ggagaacatc ggccacttcg gtctgggcta cgaggcctac acccacttca cctcgccgat
    10261 ccgccgctat ccggacctgc tggtgcatcg cgccatcaag gccgtgttgc atggcaagaa
    10321 atacacgccg gggatgaagt gggaagagct gggcgcacat tgctcgatga cagagcgtcg
    10381 tgccgacgac gccacgcgcg atgtcgaggc atggttgaag tgtttctaca tgcgcgacca
    10441 tctgggcagc gtgttcaccg gcaccgtgtc ctcggtcacc ggcttcggca tgttcgtgtc
    10501 gctggacgat ctgtatgtcg aaggtctggt gcatatctcc gagctgggca aggactacta
    10561 ccacttcgac gccgccaagc accagctgct gggcgagcgc accggcaacc gctatcgtct
    10621 gggcgaccgc gtgcaggtac gcgtggtgaa ggtcgatatg gaaagcacca aggtcgattt
    10681 cgtgctgcac gatgctgctc cagcagtgga gagcgaggat gagccgaaga aatccgacgc
    10741 gaagaaatcc ggccctaaaa aatcctccgg agggaagaag aagcatggct gatttgcgtc
    10801 tgatctacgg cttccatgcc gtcacctcgc gtatccgcca gaatccggac ggcgtgcagg
    10861 aaatctatct gcaggcgcag cgtcaagatc cgcgcatgcg cgacctgatc aagctggccg
    10921 agaccaatgg cgtgcgcatc attccggtgg acgccaagcg tctcgacggc atggcgggca
    10981 acgagcgtca tcagggcgtg gcggcaaggg tcgatgcggc gcagcgcgtg cagcatctgg
    11041 acgacgtgct ggacacgctg accgagccgc cgttgctgct ggtgctggac ggcgtgcagg
    11101 acccgcacaa cctcggcgcc tgtctgcgca gcgcggacgc gttcggcgtg catgcggtga
    11161 tcgcgcccaa ggatcgcgcg gtcgggatca atgcgaccgt cgagaaggtc gcctgcggcg
    11221 cggccgagac cgttcctttc atcaccgtca ccaatctggc ccgcaccctg cgcgagttgc
    11281 aggagcgcga catctgggtg gtgggggcgg caggcgaggc cgagcaggat ctgcacggct
    11341 tcaaacacaa gggcgggctg gcctgggtgc tgggcgcgga aggcgagggc ctgcgccgcc
    11401 tgacgcgcga gacctgcgac gagctggtgc gcatcccgat gctgggcagc gtggaaagcc
    11461 tgaacgtctc ggtcagcgcc ggcatctgtc tgttcgaggc gcgccgccag cgacagggtg
    11521 gggtgtgagc cccgggtacc cgtcggcgta ctcctcgcgg gtgcaagcgg gcgagctgaa
    11581 gctgagtcag ttcgactcgt cgatcacata gccagaatca ggaaagccga tgaagaagaa
    11641 aaaaaagtcg ttacgcgttc cggtcacccg tggcctgaag gatatctatg cgatggacat
    11701 gcataccgcc tatcaggccg cctgcatggg gcaattcaac gtggacgcct tcgggcggct
    11761 ggcggcggcg atctcggtga tccgctcggc gctcgagcag aatcgcacgc gcattcccga
    11821 tgctgttgag acgatggatg cggccatcgc gacgctgctg gcggtgcgtg cgcggggcga
    11881 tgaaaccggc cggtgggaaa tcgccgaggc ggagcgtccg tcggtgctgg ccggaatcgg
    11941 aatggccgag cagagcatcg gcacgctgga cgtcgcgctg ctggcacaaa cggccgacga
    12001 tctgctggcc tctatctccg gctaagctcg cggttcgctc catttggtca cggtatagcg
    12061 tgacagcctc acgtcatccg accagaaatc ctccggcaag ccggccttgc gtttcagatg
    12121 cgccatgaac tggcgcggca gaggcagctg ttcccatacc tgcggcagga aggtgctgcg
    12181 gtagcggccg tattcgaaga ccaggccgtc cacgccgggg cgcagctgcg ccagggcgtc
    12241 ggcctcgtcc tgcacggtca tcggctgcgg cggcgagagc agcgagacct ccacccgcgt
    12301 gtagcctaac tcttcccgtt tcaacggcat gaagcgcgga tcctcgaagg cggcggcgcg
    12361 ggcgttgtga tagacatcct ccgccagcgg acggtgggct tccagcgtgc cgatgcagcc
    12421 gcgcaactcg ccgtgctgcg tcaatgtgac gaaggtcgct cccggttgct gcagccagtc
    12481 ttcctgcggc acggccagat gcggcgcacc cagcgcttcc gcaatcgagc cgcgcgcgat
    12541 gtcgagcagg atcttgccgc gtcgttggtc gaatgccggg tcactcatcc cggtctcctg
    12601 cggtgaaggc gagggcggca tagcccacca cctgatcgcg cgatcccgcc gtatcgccgg
    12661 agttgcgcag gtcgagcaga tgcggggtga ggcggtgccg gtgcgccgca aggatcaggc
    12721 cgttgatcgg cgtggcgccg caggcctgtt cgtgcgagag cggttgttgc agttgcagca
    12781 tggattgcac ggtctgttta tccacgtgcc gggcggtggt gtagggcagg taatgcgaca
    12841 ggtcggagct gatcacgatc agcgtctcct cgccgcccca tagtctctcc agcacctcgg
    12901 cgacttcttc cgccgtcgcc atgccgacgg ccagcggcaa cagggtgaag tccttcagaa
    12961 cggattgcag aaagggcaga tgcacctcca gagaatgctc ctgcgcatgg accagcggac
    13021 tgatgcttac ctgcggcagg tccgcgatgg cccgagccgc tgccgcgtcc accgtgatcc
    13081 gtcccagcgg ggtatcgaag gcgtcggtgc cgggcagcgc caggccgcgt atcgcgacgc
    13141 ggtgggtggg gcctagcagt atcacgcggc ggatgcggtc ggcgacgggg agcaaggtgg
    13201 cataggcgct cgccgcgatg gcgccggagt agatgtagcc ggcgtgcggt gcgatcagcg
    13261 ccttgggaat cagatcatga ggcgtcgcct cgttcaacag ctcgcccaga tcatgggcga
    13321 gctgccgggg atcggcgggg tagaaaattc cggcgacggc gggggggcgg acggaagaca
    13381 tggcgttctc ctatcttgcg aggtgcataa catgagggat tatggcgaaa gaatcaagtg
    13441 gcggcggcag ggcgcaccgt tcgcctgccg cacagatttc gcgaacccgg tcccgtgcgg
    13501 tagaatgctg cgccatgaat accgttcaac tttccgtcgt cattcccgtc tataacgagg
    13561 agcagggcct gcaggctttg ttcggccgcc tgtacccggc gctggacaag ctcggcatcc
    13621 gttacgaggt cgtgttcgtc aacgacggca gccgtgaccg ttccgccgcg attctgcgcg
    13681 agcagttcca gctgcgtccc gatgtgacgc gcgtggtgct gttcaacggc aacttcggcc
    13741 agcacatggc gatcatggcc ggcttcgaac acagccgagg gcagcgcgtg gtgacgctgg
    13801 atgccgactt gcagaatccc ccggaagata tcggcgtgct gctggcgaag atggacgagg
    13861 ggcacgacta tgttggctcg atccgccgcc agcgcaacga cagcgcctgg cgtcactatg
    13921 cgtcgcgcgc gatgaatcgg ctgcgcgagc gcatcacccg catcaagatg accgatcagg
    13981 gctgtatgtt ccgcgcctac gatcgcaaca tcatcgatgc ggtcaattcc tgccacgagg
    14041 tgaatacctt catcccggcg ctggcctaca ccttcgcgcg caatcccgcc gaggtggtgg
    14101 tggggcacga ggagcgcgcg gcgggcgaat cgaaatattc cctgtacagc ctgatccgtc
    14161 tgaacttcga cctgatgacc ggcttctcgc tggtgccgct gcaatggttc tccatggcgg
    14221 gcatgctgat ctcgctgttc tccggcctgt tcttcgtgct gctggtgttg cgccgtctgg
    14281 tgataggtcc cgaggcggaa ggcctgttca cgctgttcgc gctgatgttc ttcatgatcg
    14341 gcatcgcgct gttcggcatc ggcctgctgg gcgaatacgt cgggcgcatc tatcagcaag
    14401 tgcgccaccg tccccggtat ctggtggaag cggtgcttga aatcgaggat cgaggatcga
    14461 ggatcgcgga gcgaggattg gggagcgcgg aaaaaacagc ggttgcggga gagcctgatg
    14521 tggcggaacc cagaaagaga aaagccaagc ctgtcgagcc ggtgcatccc tcgttgttct
    14581 cggataccga ggaatgagcc gggcagtcgt cttcgcctac cataacgtgg gctaccgctg
    14641 cctgagcgtc ttgctcgcgc agggcgtgga tgtcgcattg gtcgtgacgc atcgcgacaa
    14701 tcccaaagag accatctggt tcgagagcgt gcagaagctg gcggagttgc acggcattcc
    14761 gaccatcacg ccggacaatc cgaacacccc cgacgtgacc gaacaaatcg ccgccttgca
    14821 gcccgatttt ttcttctcgt tctactaccg cgagatgctc aagcgcgagt tgctggagat
    14881 tcccaagcgg ggcgcgctga acatgcacgg ttcgctgttg ccgaaatacc gtggccgcgt
    14941 gccggtgaat tgggcgataa tctgcggcga gaccgagacc ggcgcgacgc tgcactacat
    15001 gaccgaaaag ccggacaacg gcgacatcgt ggcgcagcag gcc
//
LOCUS       DASUIV010000044         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1033418, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000044 DASUIV010000000
VERSION     DASUIV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033418"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(481..1211)
                     /gene="istB"
                     /locus_tag="VFW59_01515"
                     /pseudo
     CDS             complement(481..1211)
                     /gene="istB"
                     /locus_tag="VFW59_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
     gene            complement(1211..2710)
                     /gene="istA"
                     /locus_tag="VFW59_01520"
     CDS             complement(1211..2710)
                     /gene="istA"
                     /locus_tag="VFW59_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX5362924.1"
                     /translation="MIGVELIGQIRRAFFEQRRAIKEISRELRVSRTTVRKVVRSDRT
                     AFKYAREVQPSPKLGEWIGPLTEIVEAEARLPKRERRSTQRLFEELRGRGYDGAHDSV
                     HRFVKEWREERARVPVQAFVPMSFAPGEAYQFDWSHETITLQGLPLMVKAAHMKLSHS
                     RMPFVRTYFRETQELVFDAHDRAFAFYGGACRRGIYDNMKTAVEAIFIGKARQYNRRF
                     LQMCSHHLIEPVACSPASGWEKGQVENQVGNLRDQLFRPKPRVSSLAELNGWLEDQCV
                     AYAKRTRHPEFKDRTIWEVFEEERPSLMERRAPFDGFVEKAVRATTTCLITADHNRYS
                     VDARAAGRMVLVRCHAERLVVLLDEAVVADHVRSFKRDQVIYDPWHYLPVLLKKPGAL
                     RNGAPFKDWGLPPALAQVRQRLKHHPDADRQFIKVLGGVLDHGLDAVEAACAEALSAG
                     IANGDVILTVLARQRQPPPVPSITTPHALRLKIEPVADCGRYDSLRKVA"
BASE COUNT          547 a          972 c          914 g          582 t
ORIGIN      
        1 actttgtacg gcgcacttag aaagactggt ttgagtttgc ggttggccaa agcgcttagg
       61 cgaggaaagt cacaaaccgc tggtcagtgc aacggccacc gcggcttgtt ctcgcgtttt
      121 gcggccctgg gtttaactgg aataccggat cggttctttt tctgcgaccg aacggattgc
      181 gccggactag cttccgctct gttgtgaatg ggggtcgtaa ccaaggtctg ccagtccgcg
      241 ccgacctgct ttgcttccag cttcaggacc ccagcctgtc aattcgcttc caaccgtgac
      301 ccccttttcg cgtccaatcg tgacccctgt gggacgacag gaatgggctt atccccgtag
      361 tgcacaggag ggacccgcgc gcgtcgcggg agcgcctcac gcacgcggac gcggggcgcg
      421 tgggaggtcc ctgtgtaccc acgcgggata agcccgggtg ggggtctggg ggagggtttt
      481 tcagctgcgg tttttgaagc gccagctggt gttgccggtc tcgatgatgt cgcagtgatg
      541 ggtcaggcga tcgagcatgg cggtggtcat cttggcgtcg ccgaacacct gcggccaatc
      601 ggcgaaggcg aggttggtgg tgatcagcag cgatgtgttt tcgtagagct tgctgatcag
      661 atggaacagc aactgaccgc ccgattggct gaacggcaga tagccgagtt cgtcgatgac
      721 gatgagatcg tggcgcagca gtttctcggc cagcctgccg ctcttgcccg cagcctcctc
      781 ctgctcgagc tggttgacga gatcgacgag gttgaagaac cgtcccctgg cgcgagcgcg
      841 cggatcacgg cacaagcgac ggcaatgcag agatgggttt tgccggtccc ggtgccgccg
      901 atgaagatgg cgttgcgctt ggcatcgagg aacatgccgg tcgccagctc acgaacctgc
      961 ccctgatcga gcggcgtgtc ggcgaagacg aagctgtcga gatccttcaa caccgggaac
     1021 ctggcgccgc tgatccggta gctgatcgac cgggcctgac gatgggtgtg ctcggcacgg
     1081 atcaggctgg caatcagcgg atagatctca tcgcgccggg tcaggccctt gccggcgatc
     1141 tcgtcgaagc tggcgcgcat gccatagagc ttgagctcgc tcatggcgtc gagaatctca
     1201 tggcgttcca ttaggcgacc ttcctgaggc tgtcgtagcg gccacaatcg gccaccggtt
     1261 cgatcttgag ccgcagggca tgcggcgtgg tgatgctggg caccggcggc ggctggcgtt
     1321 gccgggcaag cacggtgagg atcacgtcgc cattggcgat gcccgccgat aacgcttccg
     1381 cgcaggccgc ctcgacggca tcgagcccat gatcgagcac gccgcccagc accttgatga
     1441 actgccggtc ggcgtcggga tggtgcttga gccgctggcg cacctgcgcc agggccggcg
     1501 gcagacccca gtccttgaac ggcgctccat tgcgcaaagc gccgggtttc ttcagcaaaa
     1561 ccgggaggta atgccacgga tcgtaaatca cctggtcgcg cttgaagctg cggacatggt
     1621 cggccaccac tgcttcgtcg agcagcacga cgaggcgttc agcatggcag cgcaccagca
     1681 ccatccgacc ggcggcgcgg gcatcgacgc tgtagcgatt atgatccgcc gtgatcaggc
     1741 aggtcgtggt cgcccgcacc gccttctcga caaagccgtc gaacggggcc cgccgttcca
     1801 tcaagctcgg gcgctcttcc tcgaagacct cccagatcgt ccggtccttg aactcaggat
     1861 gccttgtgcg cttggcgtag gcgacgcatt ggtcttccag ccagccattc agttcggcca
     1921 ggctgctgac ccgtggcttg ggccggaaca gctgatcacg caggttgccg acctggttct
     1981 cgacctgacc cttctcccaa cccgaggccg ggctgcaggc caccggctcg atcagatgat
     2041 gcgagcacat ctgcaggaac cgccgattgt actgccgcgc cttgccgatg aagatcgcct
     2101 cgaccgccgt cttcatgttg tcatagatgc cgcgccgaca ggcaccgcca taaaaggcaa
     2161 acgccctgtc gtgggcatcg aacaccagtt cctgcgtctc gcggaagtag gtccgcacaa
     2221 acggcatccg gctgtgcgac agcttcatgt gcgccgcctt gaccatcagc ggcaagcctt
     2281 gcagcgtgat cgtctcgtgg ctccagtcga actgataggc ttcccctggc gcaaagctca
     2341 tcggcacaaa cgcctgtacc ggaacgcgcg ctcgttcctc ccgccattcc ttcacgaacc
     2401 gatggacgct gtcgtgcgca ccgtcatagc cgcgaccgcg caactcctcg aacagccgct
     2461 gcgtcgaccg ccgctcacgc ttcggcagcc gcgcttccgc ctccacgatc tccgtcagtg
     2521 gtcctatcca ctcacccagc ttcggcgacg gctgaacctc acgtgcgtac ttgaacgcgg
     2581 tcctgtcgga gcgcaccacc ttgcgcaccg tcgtgcgcga cactcgtaac tcccgcgaga
     2641 tctccttgat cgctcggcgc tgctcgaaaa acgcccgccg tatctgcccg ataagctcca
     2701 cgccaatcac ccccgccagc ccttcccaga gccgacatag gctcaaaagg aagcgacact
     2761 ggcatagcca ccagggggtc agaattggac gcgaaaacca ccccgcaggg ggtcactatt
     2821 gcgcgcgatt tcacagcttt gcccataaag ttgactgtgc aaacggcccg cttcgaccaa
     2881 accacgaatg cgagcagcaa tcctcttcca ctctcctgcg tgtgtcattt gagatgtcct
     2941 tatctgttca gcgcacgata gttcggatga ccatcggtgc gctctcccgc acgccaacca
     3001 aaacagtttg gcata
//
LOCUS       DASUIV010000045         3808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1312451, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000045 DASUIV010000000
VERSION     DASUIV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312451"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2221
                     /gene="purL"
                     /locus_tag="VFW59_01525"
                     /gene_synonym="purI"
     CDS             <1..2221
                     /gene="purL"
                     /locus_tag="VFW59_01525"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX5362925.1"
                     /translation="LAIAPERLDEFRAMCERERCPFAVVGTAIDEDQIVVHDKEFKND
                     PVDMPLSVLLGKPPKMTRHVTRETPRLSSFDCSGVPLAEAVERVLRLPSVANKTFLIS
                     IGDRTVGGMTARDQMVGPWQVPVADVAVTLMGYNTNRGEAFAIGERTPLALINAPASG
                     RMAVGEALTNIAAARIEKIGDIKLSANWMAAAGHHGEDAALFDTVRAVGMELCPQLGI
                     GIPVGKDSMSMKTTWKEGKKSKAVTAPLSLIVSAFAPSPDARHTLTPQLVADLDTTLL
                     LIDLGQGKNRMGGSALAQVYKQVGDVAPDVDDAALLKTFFELIQSLNAEDKLLAYHDR
                     SDGGAFVTLCEMAFAAHIGLDIQLEELHGDTLRALFNEELGAVVQVRNRDLAHIQQLA
                     REAGLKSVQKIATLNETGMIEVLRGTEKRYSENAAQLQRIWSETTYQMQKLRDNPACA
                     QAEFDRLLDVKDPGLHAKLTFDLNENVSPALLRNRPKIAILREQGVNGQVEMAAAFDR
                     AGFQAVDVHMSDVISGRVKLEDFKGFAACGGFSYGDVLGAGEGWAKSILLNPRARDEF
                     AAFFQRSDTFALGVCNGCQMMSNLHEIIPGADNWAHFSRNQSEQFEARFVMVEIQPSP
                     SIFFHGMEGSRMPIVVAHGEGYADFGNAKKLKGAQELVTMRYVDNTGKPTETYPLNPN
                     GSPQGITGLTTPDGRFSIMMPHPERVFRAVQNSWYPGEWQENGAWLRMFQNARQWVG"
     gene            2296..3612
                     /locus_tag="VFW59_01530"
     CDS             2296..3612
                     /locus_tag="VFW59_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362926.1"
                     /translation="MTKPVSLRDIPQSNIFRKGDVFVLFGELFGRGYANGLVNEARKA
                     GMTVIGVTVGRRDENNALRALNEEELATAEANLGGRIINVPLMAGFDLDAPAGTPTPT
                     DLISDMTLKSWQEDKLDWAQVERCREAGVKRFKDSVAQVMAQLDGMIPDGSNAFFAHT
                     MAGGIPKVKVFLAIANRIYKGRGERFMSSRALLDSDLGKLCLMNFDEVTANTFQYLIE
                     GSAAIRARLEKTGGQVRYTAYGYHGTEILIDGKYQWQTYTNYTQGYAKMRLENVAKDA
                     WAKGIKATVFNCPEIRTNSSDIFVGVELSLFPLLRALKQEGGSAWADAQWQACRDTLQ
                     EGNSLEGLLQKIDDYNASSVMEKYRNFEAWPMDNSVELADLMIGTSEEITQMHKDRKA
                     LVTDVLSALVLEGTGPLMFFEASQPAGPVLWLNHDVIAKQLNRIHG"
BASE COUNT          761 a         1173 c         1251 g          623 t
ORIGIN      
        1 gctggcgatc gcgccggagc gtctcgacga gttccgcgcg atgtgcgagc gcgagcgctg
       61 cccgttcgcg gtggtgggaa ccgcgatcga cgaagaccag atcgtggtgc acgacaagga
      121 attcaagaat gacccggtcg atatgccgct gtcggtgctg ctcggcaagc cgcccaagat
      181 gacccgtcat gtgacgcgcg agacgccgcg cctgtcctcg ttcgattgct cgggcgtgcc
      241 gctggctgaa gcggtcgagc gcgtgctgcg tttaccgtcg gtcgcgaaca aaaccttttt
      301 gatcagcatc ggcgaccgca ccgtgggcgg catgaccgca cgcgaccaga tggtcggccc
      361 gtggcaggtg ccggtggccg acgtggccgt cacgctgatg ggctacaaca ccaaccgagg
      421 cgaggccttc gccatcggcg agcgcacccc gctggcgctg atcaacgcgc cggcctcggg
      481 ccgcatggcg gtgggcgagg cgctcaccaa catcgccgcc gcgcgcatcg agaagatcgg
      541 cgacatcaag ctgtcggcga actggatggc ggcagccgga caccacggcg aagacgccgc
      601 gctgttcgac accgtgcgcg cggtgggcat ggagctgtgc ccgcagctgg gtatcggcat
      661 cccggtcggc aaggattcga tgtcgatgaa gaccacctgg aaagagggca agaagagcaa
      721 ggccgtcacc gcgccgctgt cgctgatcgt ctccgcgttc gcgcccagcc cggatgcgcg
      781 ccacacgctc acgccgcaac tggttgccga tctggacacc acgctgctgc tgatcgatct
      841 gggacagggc aagaaccgca tgggcggctc ggcgctggcg caggtgtaca agcaggtggg
      901 cgatgtcgcc ccggacgtgg acgatgccgc gctgctgaag accttcttcg aactgatcca
      961 gagcctgaac gcggaagaca agctgctggc ctatcacgac cgttccgacg gcggtgcctt
     1021 cgtcacgctg tgcgagatgg cgttcgccgc gcatatcggt ctggacatcc agctggaaga
     1081 gctgcacggc gacacgctgc gcgcgctgtt caacgaagag ctcggtgcgg tggtgcaggt
     1141 gcgcaaccgc gatctggcgc atatccagca actggcgcgc gaggccggcc tgaagagcgt
     1201 gcagaagatc gccacgctca acgagaccgg catgatcgaa gtgctgcgcg gcaccgagaa
     1261 acgctactcc gagaatgcgg cgcagctgca acgcatctgg tcggagacca cctaccagat
     1321 gcagaagctg cgcgacaatc cggcctgcgc acaagccgag ttcgaccgtc tgctcgacgt
     1381 gaaggacccc ggcctgcatg cgaagctgac ctttgatctg aacgagaacg tctcgcccgc
     1441 gctgctgcgc aaccggccga agattgcgat cctgcgcgag cagggcgtga acggccaggt
     1501 ggagatggct gcggcattcg atcgcgccgg attccaggcg gtggacgtgc acatgagcga
     1561 tgtcatcagc ggtcgcgtca aactggaaga cttcaagggc ttcgcggcct gcggcggctt
     1621 ctcctatggc gacgtgctgg gggcgggcga gggctgggcg aaatccatcc tgctcaaccc
     1681 gagagcgcgc gacgagttcg ccgcgttctt ccagcgctcc gacaccttcg cgctgggcgt
     1741 gtgcaacggc tgccagatga tgagcaacct gcacgagatc atccccggtg cggacaactg
     1801 ggcgcacttc tcgcgcaacc agtccgagca gttcgaggcg cgcttcgtga tggtggagat
     1861 ccagccatcg ccgtccatct tcttccacgg catggaaggc agccgcatgc ctatcgtcgt
     1921 cgcgcacggc gaaggctatg ccgatttcgg caatgcgaag aaattgaagg gcgcgcagga
     1981 actggtgacg atgcgctatg tcgataacac cggcaaacct accgagacct atccgctcaa
     2041 cccgaacggt tcgccgcagg gcatcaccgg gctgaccacg ccggacggcc gcttcagcat
     2101 catgatgccg catcccgagc gggtgttccg cgcggtgcag aactcgtggt atccgggcga
     2161 gtggcaggag aacggcgcat ggctgcgcat gttccagaac gcaaggcagt gggtggggtg
     2221 attggattca tcaccggtag ccggtgagca ggcaacagac agttaagacc aatatcaata
     2281 atcggagagt gttacatgac caagccagtt tcgttgaggg atattccgca gagcaacatc
     2341 ttccgcaagg gcgacgtatt cgttctgttc ggcgaactct ttggccgggg ctacgccaac
     2401 ggtctggtga acgaggcacg caaggcgggg atgaccgtca tcggcgtcac cgtcggacgc
     2461 cgcgacgaga acaacgcgtt gcgcgcgctg aatgaagaag agctggcgac agccgaagcc
     2521 aatctgggcg gacgcatcat caacgtgccg ctgatggccg gtttcgatct ggacgcgccc
     2581 gcaggcacgc cgaccccgac cgacctgatc tcggacatga cgctgaagag ctggcaggaa
     2641 gacaagctcg actgggcgca ggtcgagcgc tgccgcgaag ccggcgtcaa gcgcttcaag
     2701 gactcggtcg cccaggtgat ggcccagctc gacggcatga tcccggacgg cagcaacgcc
     2761 ttcttcgctc acaccatggc cggcggcatc cccaaggtga aggtgttcct cgcgatcgcc
     2821 aaccgcatct acaagggacg cggcgaacgc ttcatgtcct ctcgcgcgct gctcgacagc
     2881 gacctgggca agctgtgcct gatgaacttc gacgaagtca ccgcgaacac cttccagtat
     2941 ctgatcgaag gcagcgcggc gatccgcgcg cgtctggaga agaccggcgg gcaggtgcgc
     3001 tacaccgcct acggctatca cggcaccgag atcctgatcg acggcaagta ccagtggcag
     3061 acctacacca actacaccca gggctacgcc aagatgcgtc tggagaatgt tgccaaggac
     3121 gcctgggcga agggcatcaa ggccaccgtg ttcaactgcc ccgagatccg caccaactcc
     3181 tccgacatct tcgtcggcgt cgagctctcg ctgttcccgc tgctcagggc gctcaagcag
     3241 gaaggcggca gcgcctgggc cgatgcgcag tggcaggcct gccgcgacac gctgcaggag
     3301 ggcaactcgc tggaggggct gctgcagaag atcgacgact acaacgccag cagcgtgatg
     3361 gagaagtacc gcaacttcga agcctggccg atggacaact ccgtggagct ggccgacctg
     3421 atgatcggca cctcggaaga gatcacccag atgcacaagg accgcaaggc gctggtgacc
     3481 gacgtgctca gcgcgctggt gctggaaggc accggcccgc tgatgttctt cgaagcctcc
     3541 cagcctgcag gcccggtgct gtggctgaac cacgacgtga tcgcgaagca gctgaaccgg
     3601 atacacgggt aaggcgtagc gtgagttgaa aaaggcgacc cgagggtcgc cttttttatt
     3661 gttgagggtc tagggtcaga ctgaaccagt tttcactggg tcaatacaat ctgttcagtg
     3721 accattggtg aaagctaacg tctgacataa ccggcgctgc gcggcttcat cgcgcagcgt
     3781 ctgagttgat ggatgggttg ggcttatc
//
LOCUS       DASUIV010000046         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_569474, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000046 DASUIV010000000
VERSION     DASUIV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569474"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1612
                     /locus_tag="VFW59_01535"
     CDS             80..1612
                     /locus_tag="VFW59_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5362927.1"
                     /translation="MSAPTTAPAYRIIPIIPALLPGAMEGKGPFVASDKINEAIGFPG
                     TLVDDWHDKAIAKMGELLGKYRSLKVYMDACVHCGACSDKCHYFIGTGDPKNMPVARQ
                     DLMRSVYRRYFTLPGKLFPKLVGARDLTREVLDEWYNYFHQCSECRRCSVFCPYGIDT
                     AEVTMAAREILDSVGYGQKYSNEIIGKVHKIGNNLGLPGPALLDTLEGLEEDVKDATG
                     IDVRFPIDVKGAEVLLITPSADFFAEPHIDSLIGYAKVFHASGTSWTLSSMASEAGNF
                     GMFIGNYEQLRKIALRIREAALELGVKRIVVGECGHAWRVAYSFWNTLSGVGDGASEV
                     DRFAQMLQKQLDHRYKQPTHICEFTWDLIERNALTFDKSANDKHIITFHDSCNVARGS
                     RMGDFAGGQFEIPRNIIKAVANNFVEMDPRTTREQTFCCGGGGGLLTDDLIDLRIKGA
                     LPRMEVLQQVKDEHGVNFMATICAICKAQFTKVLPVYGFDRRMVGGVHQLVSNAIILG
                     DK"
     gene            1636..>2174
                     /locus_tag="VFW59_01540"
     CDS             1636..>2174
                     /locus_tag="VFW59_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEX5362928.1"
                     /translation="MSATTATPQPITFRRYKDGDTKVRRLSDKIFQASHSYKCPTYIQ
                     STPPCQGSCPAGEDIRGYLAIVRGTEKVPNGADGKPVMPWQEYAWRRLTEANPFPSVM
                     GRVCPAPCETGCNRNEVEDHVGINSVEHFLGEYAIANNLKFNKPAVASSGKKVAILGG
                     GPAGLSCAYQLTLKGHEVT"
BASE COUNT          458 a          638 c          656 g          422 t
ORIGIN      
        1 ctcccacccg caatcaaacg gataacccgc gcgagaaacg ccatatttcc gcctgggcta
       61 aagatctgga gtcagataaa tgagtgcacc gactaccgct ccggcctatc gcatcatccc
      121 catcatcccg gccctgctgc ccggagcgat ggaagggaag ggcccgttcg tcgccagcga
      181 caagataaac gaggcgatcg gtttcccggg aacgttggtt gatgactggc acgacaaggc
      241 aatcgccaag atgggcgagc tgctgggcaa gtaccgttcg ctgaaggtgt acatggatgc
      301 ctgtgtgcat tgcggcgcct gctccgacaa gtgccattac ttcatcggta cgggtgatcc
      361 gaagaatatg ccggtggcgc gtcaggacct gatgcgcagc gtgtatcgcc gctacttcac
      421 gctgcccggc aagctttttc cgaagctggt cggcgcgcgc gatctgacgc gcgaagtgct
      481 ggacgagtgg tacaactatt tccaccagtg ttccgaatgc cgccgctgct cggtgttttg
      541 tccctacggt atcgataccg ccgaagtgac catggcggcg cgcgagatcc tcgattccgt
      601 tggctacggc cagaaatatt ccaacgagat catcggcaaa gtacacaaga tcggcaacaa
      661 tctcggcctg cccggccctg cgctgctcga tacgctggaa ggtctggaag aagacgtgaa
      721 ggacgcgacc ggcatcgatg tgcgtttccc gatcgacgtg aagggtgcgg aagtgctgct
      781 gatcacgccg tccgccgact tcttcgccga gccgcatatc gacagtctga tcggctatgc
      841 caaggtgttt catgccagcg gtaccagctg gacgctgtcg tcgatggctt ccgaagcggg
      901 taacttcggc atgttcatcg gcaactacga gcagctgcgc aagatcgcgc tgcgtatccg
      961 cgaggccgcg ctggagctcg gcgtgaagcg catcgtggtg ggcgagtgcg gccatgcctg
     1021 gcgcgtggct tacagcttct ggaacacctt gagcggtgtg ggcgacggcg ccagtgaagt
     1081 ggaccgtttc gcgcagatgc tgcagaagca gctcgaccac cgttacaagc agccgaccca
     1141 tatctgcgaa ttcacctggg atctgatcga gcgcaacgcg ctgaccttcg acaagtcggc
     1201 caacgacaag cacatcatca cgttccacga ttcctgcaac gtcgcgcgcg gttcgcgcat
     1261 gggcgacttc gccggcggtc agttcgagat tccgcgcaac atcatcaagg cggtcgcaaa
     1321 caactttgtc gagatggatc cgagaacgac gcgtgagcag accttctgct gcggcggcgg
     1381 cggcggtctg ctgaccgacg atctgatcga tctgcgtatc aagggcgcat tgccgcgcat
     1441 ggaagtgctg cagcaggtga aggacgagca cggcgtcaac ttcatggcga ccatctgcgc
     1501 catctgcaag gcgcaattca ccaaggtgct gccggtctac ggattcgata gaagaatggt
     1561 tggcggcgta catcagctgg tcagcaacgc gatcatactg ggcgataagt aataattttc
     1621 tggaggtagg acgatatgtc agcaacaact gcaaccccgc aaccaattac tttccgtcgt
     1681 tacaaggatg gcgataccaa ggtgcgccgc ttgagcgaca agatttttca ggcgagtcac
     1741 tcgtacaagt gcccgaccta tatccagtcc acgccgcctt gccagggcag ctgcccggcg
     1801 ggtgaagata tacgcggtta tctggctatc gtgcgcggca ccgagaaggt gccgaacggt
     1861 gcagacggca agccggtcat gccttggcag gaatatgcat ggcgtcgcct gaccgaggcc
     1921 aacccgttcc cgtctgtgat gggccgcgtc tgcccggctc cctgcgagac tggctgcaac
     1981 cgcaacgaag tggaagacca tgtaggcatc aattcggtcg agcatttcct cggcgaatat
     2041 gcgatcgcca acaatctgaa attcaacaag cctgcggttg cctcttccgg caagaaggtc
     2101 gcgatcctcg gcggcggccc ggccggtctg tcctgcgcct atcagctgac gctgaagggt
     2161 cacgaagtca ccat
//
LOCUS       DASUIV010000047         5577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_11838, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000047 DASUIV010000000
VERSION     DASUIV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11838"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VFW59_01545"
     CDS             complement(<1..1357)
                     /locus_tag="VFW59_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX5362929.1"
                     /translation="MQLNFDNRFVRELPGDDGGGLSRQTPGVCWARAKPEPVGQPRLL
                     AYASEVSTLLNLTDADVHREDFLAAFSGNALLPGMVHYATRYGGHQFGQWAGQLGDGR
                     AIGLGEVVNRAGERWELQLKGAGPTSYSRRADGRAVLRSSLREFLCSEAMHHLGVPST
                     RALCLIGTGEQVMRDMLYSGDVRPEQGAIVCRVAPSFIRFGNFEMLASRNELELLRRL
                     VDFTIDRDFPMLEGAAPERFAGWFRLVCARTASLMVEWLRVGFVHGVMNTDNMSILGL
                     TLDYGPYGWIDNWDPGWTPNTTDAAGRRYCFAQQPAMARWNLERLADALMAVAPSCAV
                     LEEGLAHFDATFQSGLSAMLAAKFGLLAWQDEDGELVETLFELMQRAEIDMTEFFRAL
                     AEIDLDDPRLETLHAAFYRQALYLEHGPAIRQWLQRYAARLSTQADSREQRRARMNRV
                     NP"
     gene            complement(1341..1706)
                     /locus_tag="VFW59_01550"
     CDS             complement(1341..1706)
                     /locus_tag="VFW59_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362930.1"
                     /translation="MFVLRCFIIRRKSLFLTRWKGVQIVFNLFGEKPEHPLYDLAEAK
                     RLLAELPQDDPHKALEDIVFWLETIKSVPGFRKLTRLFGEGTTSRWDSSGWAWPLASH
                     VDHGMDATRKRIISDAAEL"
     gene            2208..2435
                     /locus_tag="VFW59_01555"
     CDS             2208..2435
                     /locus_tag="VFW59_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362931.1"
                     /translation="MRPKTLLLFVRITVSSRRQAKTGTLTMRSLDLPYQLSLESKTPP
                     IICFHVTLHRCNRIKPLTSKAGYYETTGDAS"
     gene            complement(2682..3236)
                     /gene="efp"
                     /locus_tag="VFW59_01560"
     CDS             complement(2682..3236)
                     /gene="efp"
                     /locus_tag="VFW59_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5362932.1"
                     /translation="MKTAQELRAGNVINVSGDPMVILKAEYSRSGRNGSVVKMKMKNL
                     LTEAAKETVYAADDKFEQIILDRKECTYSYFADPMYVFMDEEYNQYEIEAESMGDTIN
                     YLEDGMPCEVVFYNEKAISVELPNTIVREVVYTEPSVRGDTSGKVMKPAKISTGFELP
                     VAAFIEIGDKIEIDTRTNEFKKRA"
     gene            complement(3239..4489)
                     /gene="earP"
                     /locus_tag="VFW59_01565"
     CDS             complement(3239..4489)
                     /gene="earP"
                     /locus_tag="VFW59_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEX5362933.1"
                     /translation="MRISCDIFCNVIDNYGDIGVCWRLARQLANEYDLPVRLWVDDLA
                     SFAKLCPEADAALDSQQCRGVAVRRWCVPFPAVRPAGLVIEAFGCKLPQVYVEEMAAQ
                     EQKPVWINLEYLSAEDWVHGCHKLPSPHPGLPLTKYFFFPGFTRQTGGLLLEHDLLER
                     RDAFENDVAQQHAFWQSIGMDMPDADMLKVSLFGYENDALDRLFDSWVAGAQRVLCLV
                     PEGRILPQVGQFFADVAPRAGSHYECGNLHVRVLPFVEQERYDELLWACDVNFVRGED
                     SCVRAQWAGKPFVWQIYPQHDAVHWDKLQAFLNLYSAPLGAAASQALQDLWRAWNGEA
                     GAEVLADSPDPLQAGSAASISPGQAASTDSGQAWLEFIAVRGELGVRAQGWARELAKN
                     NLALNLLDFSRGIGRMRAFEIEGQ"
     gene            4663..5004
                     /locus_tag="VFW59_01570"
     CDS             4663..5004
                     /locus_tag="VFW59_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362934.1"
                     /translation="MVINSATVSASYSPASVSEVTRTNAQPSVDKVQAAQAQQPSTVV
                     KLSTQGQTLSLSDRPGSTGESTESRVKEGGETSSVQFQEGEGRAAAPAPQAAPAAASP
                     AIAAYAQVAAQ"
     gene            complement(5082..>5577)
                     /locus_tag="VFW59_01575"
     CDS             complement(5082..>5577)
                     /locus_tag="VFW59_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5362935.1"
                     /translation="VIRILTEQLKERHGREDFTLISQEQALEVLSSVLDILTFAVGAL
                     GGISLLVGAVGILTIMTMAVTERTAEIGLLRALGARERQILILFLGEAILLSALGGLF
                     GLALGIGIAQGAHLLFPALPVHTPWLYAVIAEITAITIGLAAGVAPAMRAAKLDPVEA
                     LHAE"
BASE COUNT         1204 a         1802 c         1524 g         1047 t
ORIGIN      
        1 ggggattgac ccggttcatg cgcgcacgac gctgctcgcg actatcggcc tgcgtgctca
       61 ggcgggccgc atagcgctgc aaccattgcc gtattgccgg gccgtgttcg aggtagagcg
      121 cttgccggta aaaggcggca tgcagggttt ccagcctggg atcgtccaga tcgatttccg
      181 ccagcgcccg gaagaactcg gtcatatcga tttcggcgcg ctgcatcagc tcgaagaggg
      241 tctccaccag ttcgccgtcc tcgtcctgcc atgccagcaa accgaacttc gccgccagca
      301 tggcggacag accggactgg aatgtggcgt cgaaatgagc cagcccttcc tcgagcaccg
      361 cgcaagatgg ggcgacggcc atgagcgcgt cggccagccg ctcaaggttc cagcgcgcca
      421 tcgccggctg ctgggcgaag caatagcgcc gccccgccgc atcggtggtg ttgggtgtcc
      481 agcccggatc ccagttgtct atccagccat aaggcccgta gtccagcgtc aggccgagaa
      541 tggacatgtt gtcggtgttc atcaccccgt gcacaaagcc gacacgcagc cactccacca
      601 tcaggctggc cgttcttgcg cagaccagcc ggaaccagcc ggcgaagcgc tcgggcgcgg
      661 caccctccag cattggaaaa tcgcggtcta tggtgaagtc gaccagccgc cgcaacagct
      721 ccagctcatt ccgcgaagcc agcatttcga aattgccgaa acggatgaag gatggcgcga
      781 cccggcaaac gatcgcgccc tgctcgggcc ggacatcgcc gctgtataac atgtcgcgca
      841 tgacctgctc gccggtgccg atcagacaga gcgcccgcgt gctgggcacg ccgaggtgat
      901 gcatcgcctc gctgcacagg aactcccgga gggatgagcg caacaccgcg cggccatccg
      961 cccggcgcga ataggacgtg gggcccgccc ccttgagctg cagctcccag cgctcgcctg
     1021 cccggttgac cacctcgccc agcccgatgg cgcggccgtc gcccagttgc cccgcccact
     1081 gcccgaactg gtgtccgcca tagcgggtcg cgtagtgaac catgcccggc agcaaggcat
     1141 tgccggaaaa ggccgcaaga aaatcctccc tatgcacgtc ggcatcggta aggttcaata
     1201 gagtggatac ctcgcttgca taggccagca acctcggttg cccgacgggc tcaggcttgg
     1261 cccttgccca gcacacaccg ggcgtctggc gtgacagccc gcccccatcg tcaccgggca
     1321 attcgcgcac gaagcgatta tcaaagttca gctgcatcag agattatccg tttacgggta
     1381 gcatccatcc catggtcaac gtggctagcc aggggccacg cccagccact cgaatcccac
     1441 ctgctcgtag tcccctcacc gaatagccgc gtcagctttc ggaaaccggg cacactcttg
     1501 atcgtttcaa gccaaaagac gatatcttcc agcgccttgt gcggatcgtc ctgcggcagc
     1561 tcggccaaca ggcgcttggc ctctgccaga tcgtacaggg gatgttccgg tttctcgcca
     1621 aagagattga acacgatctg cacacctttc caccgggtta agaacagcga cttgcgccgg
     1681 atgataaagc agcgcaatac aaacaaaaag gccaaccttc cggttggcct tgttatcgaa
     1741 cggtggtgat acggtgttat tcagacatga tcacgtcgag cgtgccgcct catcatcggc
     1801 ctcacaataa cagtcgtgac gccagcggca tgcctcttcc ttttttttgt cgcaattttc
     1861 agcactcggt gtggcatagc agggaacatc gccattctcg ccctgtattt gcctgatcag
     1921 gtcggcctgt atgctaaaac gtcgctcctt ttcctgttcc atttcgcccc ccatctgtag
     1981 tcaccacgat gaattcggca gctagccgag gttcccaaaa ccacgtgctg agcatcctaa
     2041 caagctgcgg gggaaaatag aatagaccga aatatctagt cattatgtct aatgcataac
     2101 gatgaatccg aaagcgaatc tcgctgctag tcagcctcaa gcgccagcga ctgcctgact
     2161 caactctcct gactcatatc cgctgcagcg gcctcatggt gacaatcatg agaccaaaga
     2221 cattgctgct ctttgtacgc atcacagttt ccagccgtcg gcaggccaaa acagggactc
     2281 tcaccatgcg aagcctggat ctgccgtatc agctctccct ggaatctaaa acaccgccca
     2341 ttatctgctt tcatgtcacc ctccatcgct gcaatcgcat caagccactc accagcaagg
     2401 ctggatatta cgaaaccaca ggcgatgcat cctaacaaca cggggatgaa ttaaaaatat
     2461 gcctaaatat ctaggccgtt cggtctaaga gctttccgac agcgcaaaca aaaaggccaa
     2521 cctcgcggtt ggccttttgc taatccctct cctcaatcct ctggtggaac tactaaccaa
     2581 tcgactaaga ccgcaagcgg aaaaatcgct ggttatcccg caagcgggag aggaagcaat
     2641 cgactcttct cataatgaga aggggattgt tgaatccttg cttaagcgcg cttcttgaac
     2701 tcgttggtgc gggtgtcgat ttcgatcttg tcgccgattt cgatgaatgc cgcgacaggc
     2761 agctcgaaac cggtgctgat cttcgccggc ttcatcacct tgccggaagt gtcgccgcgt
     2821 accgatggct cggtgtaaac gacttcacgc acgatggtgt tcggcagttc gacggaaatc
     2881 gctttctcgt tgtagaacac cacttcgcaa ggcatgccgt cttccaggta gttgatcgtg
     2941 tcgcccatgc tctctgcttc gatctcgtac tggttgtatt cttcgtccat gaaaacatac
     3001 atcggatcgg cgaagtagga ataggtgcac tccttgcgat ccagaatgat ctgttcgaac
     3061 ttgtcgtcgg ctgcgtacac ggtttctttg gcggcttcgg tcagcagatt cttcatcttc
     3121 atcttgacca ccgaaccgtt acgaccggaa cggctgtatt ctgccttcag aataaccatc
     3181 ggatcgccac tcacattgat gacgttacct gcgcggagtt cctgtgctgt tttcatgatt
     3241 actgcccctc aatttcgaag gcgcgcattc taccgattcc cctggaaaaa tccagcaaat
     3301 tcaacgccag attgtttttc gccaattccc gcgcccagcc ttgcgcgcga acccccaact
     3361 caccacggac cgcgatgaat tcgagccagg cctgtcctga gtctgtcgaa gcggcctgtc
     3421 ctgggcttat cgaagcggct gagcctgcct gcagcgggtc aggcgaatcg gccaacactt
     3481 ctgccccggc ctcgccattc cacgcccgcc acaagtcttg cagcgcctga ctggccgccg
     3541 caccgagcgg cgctgaatac agattcaaaa aagcctgcag cttgtcccag tgcaccgcgt
     3601 cgtgctgagg atagatctgc cacacgaagg gcttgcctgc ccactgcgca cgcacgcaag
     3661 aatcctcacc gcgcacgaaa ttgacatcgc aagcccacaa caattcgtca tagcgctcct
     3721 gctcgacgaa cggcagcacg cgcacatgca ggttaccgca ttcatagtgg ctgccggcac
     3781 gcggtgcgac atcggcgaag aactgcccga cctggggcag gatgcgccct tccggcacca
     3841 gacacagcac gcgctgcgca cccgcgaccc agctgtcgaa cagtctgtcc agcgcatcgt
     3901 tctcataacc gaacaacgac accttgagca tatccgcatc gggcatatcc atgccgatgg
     3961 attgccagaa agcatgctgt tgcgcgacat cgttctcgaa cgcatcgcgt cgctccagca
     4021 gatcgtgttc cagcaacaat ccgccggtct gccgggtgaa accggggaag aagaaatact
     4081 tggtcaaggg caagccgggg tgaggcgaag gcagcttgtg gcaaccatgc acccagtctt
     4141 cggcggaaag atattcgagg ttgatccaca ccggcttttg ctcctgagcc gccatctcct
     4201 cgacatagac ctgcggcaac ttgcagccga acgcctcgat caccagtccg gcaggccgaa
     4261 cagcgggaaa tggaacgcac cagcgccgca ccgccacccc ccgacattgc tggctatcca
     4321 gcgccgcatc ggcctcgggg cagagcttgg cgaagctagc cagatcgtcc acccacagcc
     4381 gcaccggcag gtcatattcg ttcgccaact gccgcgccag ccgccagcac acgccgatat
     4441 cgccgtagtt gtcgatgaca ttacaaaaga tgtcgcatga aatccgcact gcatcccctc
     4501 aggccgcaag cggcgctgta gccctccgca ctgcatgcca tgcaaaattg atctgaacag
     4561 ggccgaattg ccagcccgat aaaattgtaa gacaatcacg cctgcgttcg cctgaattct
     4621 ggcccgtgac gcaaaaccat catacgcata gcgagagata aaatggtcat caacagcgca
     4681 accgtcagtg ccagttactc acccgcctcg gtctccgagg tcacccgaac gaatgcgcag
     4741 ccgagcgtcg acaaggtaca ggccgcgcag gcccaacagc cctccaccgt cgtcaagctc
     4801 tcgacacagg gacagacctt gagcctgagc gaccgtccgg gatcgacggg tgaaagcacc
     4861 gaatccaggg tcaaggaagg tggcgaaaca tccagcgttc aattccagga aggcgaaggc
     4921 cgagcagccg cgccagcccc ccaagcggcg ccagccgccg cgtcaccggc aatcgccgcc
     4981 tacgcgcagg tcgcagccca gtagacctca ccgcgcctcg gcatgaggcg cggctccccc
     5041 gattcaaacc acaaccagta ggggcacggc gcgccgtgcc cctactccgc atgcagcgcc
     5101 tcgaccgggt cgagtttggc ggcgcgcatc gccggggcca cgcctgcggc caggccgatg
     5161 gtaatggcgg tgatttcggc gatgacggcg tacagccagg gggtatgcac cggcagcgca
     5221 ggaaacaaca ggtgtgcgcc ctgcgcgatg ccgatgccca gcgcaagacc gaacagtccg
     5281 cccagcgccg agagcaggat cgcctcgccc aggaacagta tcaatatctg ccgttcgcgc
     5341 gcccccagag cgcgcagcag accgatctcg gcggtgcgtt cggtgacggc catggtcatg
     5401 atggtgagga tgccgaccgc gccgaccaag agcgagatgc cgcccagcgc gccgacggcg
     5461 aaggtgagga tgtcgagcac ggagctcaat acttccagcg cctgttcctg agagatgagg
     5521 gtgaagtctt cgcggccgtg gcgttccttg agctgttcgg tgaggatgcg gatgacg
//
LOCUS       DASUIV010000048        13658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1221124, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000048 DASUIV010000000
VERSION     DASUIV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 13658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13658
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221124"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..502
                     /locus_tag="VFW59_01580"
     CDS             53..502
                     /locus_tag="VFW59_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5362936.1"
                     /translation="MLTQDANPFDILLVEDEPADANLVRSALKTNKVLCTLHHEMDGV
                     DALAFLRRQGERYKDAVMPDLILLDLNMPRMNGKEFLAEIKKDPELSVIPVVVLTTSD
                     VERDVVASYKLGAAGYITKPVDMEQFIDAIRQLDNYWFVLARLPKKS"
     gene            595..2136
                     /locus_tag="VFW59_01585"
     CDS             595..2136
                     /locus_tag="VFW59_01585"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX5362937.1"
                     /translation="MQFQPGATLRDILISGGFAVRSACGGDGSCGQCQVRVAESGSIP
                     FTASERLRLSGQQLSEGVRLSCQLLPDTNLHVSLEKPVAQMSWRSLRDDEYSSLALPG
                     TPAQKMLRYGIAIDLGTTHIRLTLWDISNGERIAGLAGLNPQGSYGADVLTRLMEAAR
                     SSERARELGAVVQDAIAEALAGIAAQAGLALADVGEVWVVGNTAMLSLLAGKHYEQLL
                     QPENWTRRIDCQPDDPGFLRNLWGVSPVAAIHLVTPLGGFVGSDLLAGVIATRLIEQP
                     AGSLLIDFGTNSEMALWDGQTLHVTAAAGGPAFEGSGISCGMPGEAGAICRLSQAGEG
                     FSIQVLGDGAPRGLCGSGLVDAVAWLLRNGKLDKVGRFTDRDSEGFVLHEGEHRIVLK
                     RGDIDVFQRAKAAIGAGVRWLCQQAGMSLGELHQVYACGAFGRLLDVRCAQQIGLLPP
                     VAPEAVKLEGNSALAGCEALLLSDGGTTALDAVLAVSKVYNLAEDVGFESLFVENLYL
                     QSMQE"
     gene            2193..3884
                     /locus_tag="VFW59_01590"
     CDS             2193..3884
                     /locus_tag="VFW59_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5362938.1"
                     /translation="MLDEVIRNYNEAVMDTDRTRALQVVADAVAQGVTPEDIVFKVVI
                     PGMDMMVKALSEGFDTNLAQHFMTSQIAADVTEKMLLLFKTPPQIVGTVVIGTAAGDL
                     HTLGKRIVIGCLKAQMIDVTDLGVNVPAERFVDEAVARGAQVIGVSAMMVHTARGENG
                     ALKVRKILKERGLENKIKLVVGGAPFRYDSGLYKMVQADAWAENGVSALRVIMDCIEE
                     VKTITSMDRLVAAANGKILDHIPVFCNLLDQGAREMGMSIEEYFSNGENVAEAQLRML
                     KKFGHDNVWSLFYVGKEAELFGCHKIKFAKDGPPNVEDYIIQSYEDIANLKVPDDIST
                     HPAFAETAKCLAILKREVGGKVPICAYLTASMSLPALLMSMEKWMELLMIGDPAVRDM
                     LLEKCSDFFRKEIEAYRKAGADVLVYADPYGSTDIIPMRLFKELSLKWIKRDLEPGGL
                     DGVVYYVGGARLNTVADEVIREIGISVFYPGPLDDITESMRIIDGRALCAGVINDIKL
                     LDWTPDEVRAEVKRIVQGGLSVGNKLFFGTVVMPYGIPDENIQTMIEAAKEYGRL"
     gene            3889..4509
                     /locus_tag="VFW59_01595"
     CDS             3889..4509
                     /locus_tag="VFW59_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEX5362939.1"
                     /translation="MPNENCLLLGCGILRNEVNFLIGKNGWPLDTDWLDSSLHVNFEK
                     LAHALQTGILRNRGRDIVVFYGCCHPRMDKILDTAHTFRTEGQNCVEMLLGREKFMEE
                     LTGGAFFLLEDWALRWDEAIGKTFGDNPAVVREIFQLSNKSLLCLRTPCSGNFELAAN
                     EVGEKIGLPVRWLDVGLDQLEAVLRQAVQRKLAGTAHPKQHMNREP"
     gene            4511..6427
                     /locus_tag="VFW59_01600"
     CDS             4511..6427
                     /locus_tag="VFW59_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5362940.1"
                     /translation="MLDSLPSMTLIPHGYCISWQPGLIAALVIGNLAIALAYFTIPLV
                     ILKFIRQRSDIDFKHLHWLFAGFIVTCGVTHLLHVVELWYPVYYLEAAMDMLTALVSI
                     IAAVVLWRILPVIVSLPSSNQLRAANEELRQVGTELQDREVQLRNLGDSLPDSFLYQY
                     TLENDRPRFLYISSGVERLNGIKAEVVMQDAMALLGQIAPEQRDRYAETQTASQRDMS
                     NFAMDLHMLRADGEWRWIQVKSRPRRNNNDRVVWDGIATDITDRHLFESEINRLAQAI
                     EQNPTGILITDHQGALEYMNAACTRISGYQFAEAYAKNRTPREIISSEMSDAEYGAVQ
                     TQLQSGRTWSGVLRNRRKDGSIYWEQMTVSPIYSNEGKLASYLYLRSDVTEQRNAEAA
                     LTLRSTALERAHADLTRFADVSAHHLMEPTRRLASYAQQLRARSSALPEACHDEEMLS
                     SLAYIEQDAARLRSLVRDVQLYLAAGTPRDEVRMEDSNAVLNALKLRMAEQLESRHVS
                     LEFGALPQAMLDRPRLTDLFTVLLDNALRHGQPVYPDVAAQIRITGEREDGLSRFRVM
                     DNGGGIPVEYLERVFEIFERLSVTNSGGGTGIGLSIARRIVESRHGNIWIENLPEGGA
                     MVVFELPDGADHAE"
     gene            6417..6866
                     /locus_tag="VFW59_01605"
     CDS             6417..6866
                     /locus_tag="VFW59_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5362941.1"
                     /translation="MLNEGSNAFDILLVEDEPADALLVRTAIKQCKVLCNVSHAIDGV
                     EALDFLRRKGQRYRNAPRPDLILLDLNMPRMNGREFLAEVKADENLASIPVVILTTSE
                     VERDIETAYQLQASGFITKPVDMEQFSDAMCQLVTYWLTLTRLPKKG"
     gene            6870..8933
                     /locus_tag="VFW59_01610"
     CDS             6870..8933
                     /locus_tag="VFW59_01610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5362942.1"
                     /translation="MTIDPDCRILLVEDDESDAHLLRQSLLRAASFSFDIVWVKSLAD
                     ARRQLSEHAHDVVLLDLTLPDSSGLDTVHAGRLAAGALPLIVLTGHDDTEFALQALEA
                     GAQDYLVKGDFQEGELIRAIRYAISRAKLEQRLYEAEERWRFALEGAGDGVWDWTIQT
                     DKVAFSPRLKEMLFSSEAEVKEHLDEWKHRVHPDDQARVMADLQTYLEGKSAAYLNEH
                     RLRCSDDAWKWMLHRGMVVSRTPEGKPLRMIGTFTDIDRRKRAEEALQRSEALLKKTQ
                     QISQVGGWEYDVATQHVRWTDETYRIHELTSDFDPGEVSVNIKFYSPLDQVVLDRAFK
                     KVVEQGDAYDLELQLIGAKGTRKWVRTTGQAERVDGKITRVFGNIMDITKSKQAEELM
                     RLSSTVFQTVDEAILVSDVDNRIITVNPAFTKVTGYTAEEVIGENPRILSSGKHGEAF
                     YRVLWDQLSRDGSWEGEMWNRRKNGEIYVEWTSIKQVRDAHGKLTHYVAAFSDITTRK
                     ANEESIRHQAQYDALTDLPNRILLFDRLGQAIAQAKRDKTHLALMYIDLDKFKPINDN
                     LGHAIGDQLLQDVAYRMQDCVRAMDTVARIGGDEFVVLLPVVENEQDALRVAEKIRVA
                     LNQPFGIAGESLRISSSTGVVIYPEHGTSENELTGNADIAMYFAKESGRDNVKLFKKA
                     MRSAQ"
     gene            complement(8981..9055)
                     /locus_tag="VFW59_01615"
     tRNA            complement(8981..9055)
                     /locus_tag="VFW59_01615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9020..9022),aa:Arg,seq:cct)
     gene            9166..9636
                     /locus_tag="VFW59_01620"
     CDS             9166..9636
                     /locus_tag="VFW59_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362943.1"
                     /translation="MQARINLFFAWFFIPQTLAMGWVAASGRIVLELLGTDTREGDIP
                     GRMVGALLLLGAIYLVLHFRGSLPPEGKSDGNGYRFGHRAVLLGNVLAALLFVFQFVA
                     PLIDSYNTHLILDNFTTAFGYWAMACWVVGFSFLYQSAQLKQSTPSEQSAPSRK"
     gene            9646..11547
                     /locus_tag="VFW59_01625"
     CDS             9646..11547
                     /locus_tag="VFW59_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5362944.1"
                     /translation="MPFITLDKASLAFGHVALLDHADFQLDEGERVGLIGRNGGGKSS
                     MMRVLAGQGNLDDGLVWRAPTARICYVSQEPVLDADDSVFDAVAKGLGKLQKLLHDYH
                     HVSHQLSEPDADFDKLLEEMQVLQTQLEAQNGWSVQARIETAIAKLDLDPERRVGDLS
                     GGQRKRVALAQALVAEPDVLILDEPTNHLDFASIEWLEGLLNDFRGSVLFVTHDRRFL
                     DNVTTRIVELDRGKLASFPGNFSAYQALKERMLADEAVMNAKFDKVLAQEEVWIRQGV
                     KARRTRNEGRVLRLEQLRRERAARRDKVGKVEMTVETGERSGKLVAELTDVSKSFGGR
                     KIVDNFSCRIQRGDKIGLLGPNGAGKSTLLKMILGDLEPDSGTVKLGTKISVAYFDQL
                     REQLDEEATLADTISQGADFVEIGGQRKHVISYLGDFLFAPERARSPVKSCSGGERNR
                     LLLARLFTRPANVLVLDEPTNDLDIETLELLEELLANYDGTLFLVSHDRAFLDNVVTQ
                     VIAFEGDGKLMEYVGGYEDWVRVQKFQAAQQATKPVAAVKQPVQAAPVEKPKQSKLSY
                     KDARELEELPKRIEVLEQEQIDIAAHFADGSLFRDDPKRAKQLQARSEEIEAEVLAAM
                     ARWEELEQR"
     gene            11732..12934
                     /locus_tag="VFW59_01630"
     CDS             11732..12934
                     /locus_tag="VFW59_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5362945.1"
                     /translation="MEELKTANDVLFVLLGAIMVLAMHAGFAFLELGTVRKKNQVNAL
                     VKIISDLAISTLAYFFIGYGIAYGVSFFVGAEELAQRNGYDLVKFFFLMTFAAAIPAI
                     VSGGIAERSRFNPQLAATFMLVGFVYPFFEGIAWNHRFGIQDWLHATFGAEFHDFAGS
                     VVVHAVGGWIALAAVLLLGARRGRYNKEGRISAHPPSSIPFLALGAWILTVGWFGFNV
                     MSAQNISGISGLVAVNSLMAMVGGTLTALWFGKNDPGFVHNGPLAGLVAVCAGSDLMH
                     PIGALVVGGVAGALFVVLFTQTQNRWKIDDVLGVWPLHGLCGAWGGIAAGIFGAKALG
                     GIGGVSLMSQLAGTLLGVSIAFIGGYVVYGALKKLVGIRLEPEDEFNGADLSIHKISA
                     TPERESGW"
     gene            complement(13113..>13658)
                     /locus_tag="VFW59_01635"
     CDS             complement(13113..>13658)
                     /locus_tag="VFW59_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="HEX5362946.1"
                     /translation="KDLWEMHKLRNQVFKEQMGWDVPVMSGMEIDGYDAINPHYLMIR
                     DTLDGVRGCLRILPTEGPYMLKDTFPELLYGQKAPEDKKTWELSRFAIRSDDSRGFGF
                     SGMTLDALREIVRFGDQMGIERYVLVTTISIERMMRRSGFAITRFGPPMRIGVETTVA
                     IDFDIGEQTHEALFGKMLAAA"
BASE COUNT         2762 a         3575 c         4410 g         2911 t
ORIGIN      
        1 gtgtatctcg atacgatcat tgtgcgcacg ccgaacccgg agagtaggaa atatgttgac
       61 tcaagatgcg aatcctttcg atattctgct ggtagaagac gaacccgcag acgccaatct
      121 ggttcgttcc gcactcaaga cgaacaaggt gctctgtacc ttgcatcacg agatggacgg
      181 ggtggatgcg ctggctttcc tgcgccgcca aggggagcgt tacaaggatg cggtgatgcc
      241 ggatctgatc ctgctggatc tcaatatgcc gcgcatgaac ggcaaggagt ttctggccga
      301 gatcaagaag gatcccgagc tgtcggtgat cccggtggtg gtgttgacca cctcggatgt
      361 ggagcgcgat gtggtggcct cctacaaact gggggcggcg ggctatatca ccaagccggt
      421 ggatatggag cagttcatcg atgccatacg ccaactcgat aactactggt ttgtcttggc
      481 gcgtttgccc aagaagtcct gatatcggcg tcgaagttat ttcctcgaat cagtttggac
      541 gcaccttctc ccccgcatga tctctccgtc ctctcccccg agggagcggc gctgttgcaa
      601 tttcagcctg gcgcgacctt gcgcgatatc ctgatttccg gcggttttgc ggtgcgttcg
      661 gcttgcgggg gggacggcag ctgcggccaa tgccaggtgc gcgtggcgga aagcggcagc
      721 attcctttca ccgccagcga acgtttgcgg ctgtccggcc agcagttgag cgagggcgtg
      781 cgcctttcct gccagctgct gcccgatacg aatctgcatg tgtcgctcga aaaaccggtg
      841 gcgcagatgt cctggcgctc attgcgggat gacgagtaca gcagtctggc gttacccggc
      901 accccggcgc aaaaaatgct gcgttacggc atcgccatcg atctcggtac cacgcacatc
      961 aggctgacgc tctgggacat ctcgaacgga gagcgcatcg cggggcttgc tggcctcaat
     1021 ccgcaaggaa gttatggcgc cgatgtgctg acccggctga tggaggccgc tcgctccagc
     1081 gagcgcgcgc gcgagctcgg tgccgtggtg caagacgcga tcgcggaagc gctggccggt
     1141 atcgccgcgc aagcgggact cgcattggct gacgtgggcg aggtctgggt ggtcgggaat
     1201 acggcgatgc tcagcctgtt ggcgggcaag cattacgagc agttgctgca accggagaat
     1261 tggacgcggc gcatcgattg ccagcccgac gatcccggct ttctgcgcaa cctgtgggga
     1321 gtgagtcccg ttgccgccat ccacttggta accccgctgg gcgggtttgt cggctcggat
     1381 ttgctggcgg gcgtgatcgc cacgcgtctg atcgaacagc ctgcgggctc cctgctgatc
     1441 gatttcggca cgaattccga gatggcgctg tgggacgggc agacactgca tgtgactgcc
     1501 gcagcgggag gccccgcctt tgaaggcagt ggcatcagtt gcggcatgcc gggagaggcg
     1561 ggggcgatct gtcgcctgag tcaggccgga gagggtttct ccatacaggt gctgggcgac
     1621 ggcgcaccgc gaggactgtg cggttcaggg ctggtggatg ccgtggcatg gttgctgcgc
     1681 aatggcaagc tggacaaggt ggggcgattc accgaccggg acagcgaggg tttcgtgctg
     1741 catgagggcg agcatcgcat cgttttgaag cgcggtgata tcgatgtgtt ccagcgtgcc
     1801 aaggccgcca tcggagccgg cgttcgttgg ctatgccagc aggccggtat gagcctgggc
     1861 gagttgcatc aggtttatgc ctgcggtgca ttcggacgcc tgctggatgt gcgctgcgcc
     1921 cagcagatcg gattgctgcc gccggttgcg ccggaagcgg tgaagctgga agggaatagt
     1981 gcgctggctg gatgtgaagc gctgttgctt tcggacgggg gaacaacggc gctggatgcg
     2041 gtactggctg tcagcaaggt ttataacttg gccgaggatg tcggcttcga gtcacttttc
     2101 gtggaaaacc tttatctgca atccatgcag gaatagaatt caaccgttgc accgaacata
     2161 taagcatccg ccctgctcaa aggagttgca tcatgctgga tgaggtcatc aggaattata
     2221 acgaggccgt gatggatacg gatcggacgc gggccttgca agtcgtggcg gatgccgtag
     2281 cgcagggtgt cacgcccgag gacatcgtgt tcaaggtggt cattcccggc atggacatga
     2341 tggtcaaggc cctgagcgag ggcttcgaca ccaatctcgc gcagcacttc atgacgtcgc
     2401 agatcgcggc ggatgtcacc gaaaaaatgt tgctcttgtt caaaacgccg cctcagatcg
     2461 tcggtaccgt ggtcatcggt acggcggcag gggatctgca caccctgggt aaacgcatcg
     2521 tcatcggctg cctgaaggcg cagatgatcg acgtgaccga cctcggcgtg aatgtcccgg
     2581 cggaaagatt cgtggatgag gccgtggcgc ggggcgccca ggtaatcggg gtgtcggcga
     2641 tgatggtgca taccgcgcgc ggcgagaacg gcgcgctcaa ggtgcgcaag atacttaaag
     2701 aacgcggact ggagaacaag atcaaactgg tggtgggcgg cgcgccgttc cgttatgact
     2761 ccgggttata caagatggtg caggcggatg cctgggccga gaacggcgtc tctgcattga
     2821 gggtgatcat ggactgcatc gaagaagtca aaaccatcac cagcatggac cggctggttg
     2881 ccgcagccaa cggcaagata ctcgaccaca tcccggtgtt ctgcaatctg ctcgatcagg
     2941 gcgcgcgcga gatgggcatg tccatcgagg aatatttttc caacggcgag aacgtggccg
     3001 aagcccagct caggatgctg aagaagttcg gccacgacaa tgtctggagc ctgttctatg
     3061 tgggcaagga ggccgagctg ttcggctgcc acaaaatcaa gtttgccaaa gacggcccgc
     3121 cgaacgtgga ggactacatc atccagtcct acgaggacat cgccaacctg aaagtgccgg
     3181 acgatatcag tacccatccc gcctttgccg agacggcgaa atgcctggcg atactgaagc
     3241 gcgaggtggg gggcaaggta cccatctgcg cgtatctcac cgcatccatg tcgctccccg
     3301 ccttgttgat gagcatggag aagtggatgg agctgctgat gattggcgat ccggcggtgc
     3361 gcgacatgct gctggaaaaa tgctccgatt tcttccgcaa ggagatcgag gcttaccgca
     3421 aggcgggcgc ggatgtgctg gtgtatgccg acccttacgg ctcgaccgat atcattccga
     3481 tgaggctgtt caaggagctg tcgctcaaat ggatcaaacg cgatctggaa ccgggcggat
     3541 tggacggcgt ggtctattac gtgggcggtg cgcggctgaa caccgtggcc gacgaggtga
     3601 tacgcgagat cggcatctcg gtgttttatc ccggcccgct ggacgacatc accgagtcca
     3661 tgcgcatcat cgatggccgg gcgctgtgcg ccggagttat caacgatatc aagttgctgg
     3721 actggacccc ggacgaggtg cgcgccgaag tgaagcgcat cgtgcagggc ggtttgagcg
     3781 tgggaaacaa gttgttcttc gggacggtgg tgatgcctta cggtatcccg gatgagaaca
     3841 tccagaccat gatcgaggcg gccaaggaat acgggcggtt gtagcaatat gccaaatgaa
     3901 aattgcctgc tgctcggctg cggaattttg cggaacgagg tcaactttct catcgggaag
     3961 aacggttggc cgttggatac cgattggctg gattcgtcgc tgcatgtcaa tttcgaaaag
     4021 ctggcgcatg cattgcagac cgggatcctg cgcaacaggg ggcgcgacat tgtggtgttt
     4081 tacggctgtt gccatccgcg tatggacaag atactggaca cggcgcatac ctttcgcacc
     4141 gaaggacaga actgtgtgga gatgctgttg ggcagggaaa agttcatgga agagctgacc
     4201 ggcggtgcat tcttcctgct cgaggattgg gcgctgcgct gggatgaggc catcggcaag
     4261 accttcggcg acaaccccgc tgtggtgcgc gagatttttc agttgtcgaa taaatcgttg
     4321 ctctgcctgc gcacgccctg ctccggcaac ttcgaattgg cggcgaacga ggtgggagaa
     4381 aaaatcgggc tgccggtgcg ctggctggat gtcggtctcg atcaactgga ggcggtgttg
     4441 cgtcaggcgg tgcagcgcaa gcttgccggc acggctcacc cgaaacagca catgaacagg
     4501 gaaccttgaa atgctggaca gcttgccttc gatgacgctc attccccacg gttattgcat
     4561 ttcctggcaa ccggggttga tcgccgcgct ggtgatcggc aaccttgcta tcgcgctggc
     4621 gtattttacg atcccgctgg tcatcctgaa attcatccgg cagcggtctg atatcgactt
     4681 caagcatctg cactggctgt ttgccgggtt tatcgtgacc tgcggtgtga cgcatttgtt
     4741 gcatgtggtg gagctctggt atccggtcta ttaccttgaa gcggcgatgg acatgttgac
     4801 ggcgctggtt tccattatcg ctgcggtggt gttgtggagg attttgccgg tcatcgtgag
     4861 tctgcccagt tcgaatcaat tgcgggcggc gaacgaggag ttgcggcagg tcgggacgga
     4921 gttgcaggac agggaggtgc agctgcgcaa tctgggagac agtctgccgg acagttttct
     4981 ctaccagtac acgctggaaa atgaccggcc cagatttctt tacatcagct ccggcgttga
     5041 gcggctgaac ggaatcaagg ctgaagtcgt gatgcaggat gcaatggcct tgctggggca
     5101 gatcgcgccg gaacagcgcg atcgttatgc cgagacgcag accgccagcc aacgggatat
     5161 gagcaatttc gcgatggatc tgcacatgct tcgagccgat ggagaatggc gctggataca
     5221 ggtgaagtcg cgtccacgca gaaataacaa tgaccgggtg gtctgggacg gaatcgcgac
     5281 ggatatcacg gatcgacatt tgttcgaatc ggagatcaat cgactggccc aggccatcga
     5341 acagaatccg accgggattc tgatcaccga tcatcaaggg gcgctggagt atatgaatgc
     5401 ggcctgcacc cgtatcagcg gctaccagtt tgccgaggcc tatgccaaga accggacacc
     5461 gcgcgagatt atttccagtg aaatgagcga tgcggaatat ggcgcggtac aaacgcagct
     5521 gcagtccggg cgaacatgga gcggtgtgtt gcgcaaccgt cgtaaggacg gctcaatcta
     5581 ctgggagcag atgaccgttt caccgatcta cagcaatgaa ggcaagctgg cgagttacct
     5641 gtatctgcgt tcggatgtca ccgagcaacg gaatgccgaa gcggctttga cactccgcag
     5701 taccgcattg gagcgggccc atgccgatct tacgcggttc gccgatgtct cggctcatca
     5761 cctgatggag ccgacacggc gtctggccag ttatgcgcag caactacggg cgcgatcctc
     5821 ggctctgccc gaggcctgtc atgacgagga gatgctttct tccctggcct acatcgagca
     5881 agatgccgcg cgcctgcgta gcctggtgcg cgatgttcag ctctatctgg ccgcaggcac
     5941 acctcgtgac gaagtcagga tggaggacag caatgccgta ttgaatgcat tgaagctgcg
     6001 tatggccgag caactcgagt cgcggcatgt cagtctggag ttcggggcgt tgcctcaggc
     6061 catgctggat cgtcccaggt tgacggattt gtttacggtg ttgctggata acgccctgcg
     6121 ccacgggcaa cccgtctatc ccgatgtggc ggcacaaatc cggatcaccg gtgagcgcga
     6181 ggacggtttg agccgctttc gggtgatgga taacgggggc ggtatccccg tggagtatct
     6241 ggagcgcgtg ttcgaaatat tcgagcgcct gagcgtcacc aacagcggtg gaggaacggg
     6301 tatcggttta tccattgccc gccgcatcgt ggaaagcagg catggcaaca tctggatcga
     6361 gaacctcccg gagggcgggg cgatggttgt gttcgagttg cctgatggag cagatcatgc
     6421 tgaatgaagg atcgaatgcg ttcgatatcc tgctggtgga agatgaaccg gcggatgcgc
     6481 tgctggtgcg taccgcaatc aagcaatgca aggtcttgtg caatgtgtcc catgcgatcg
     6541 acggggtcga ggcgctcgat tttctccggc gcaaggggca acgctaccgc aatgctcccc
     6601 ggccggattt gatcttgctc gacctgaaca tgccgaggat gaatggacgt gaattccttg
     6661 cggaggtcaa ggcggatgaa aatctggcaa gcatccccgt ggtgattctg acgacatcgg
     6721 aggtggaacg agatattgag accgcctacc agcttcaggc gtcagggttt atcaccaagc
     6781 cggtcgatat ggaacagttc agcgacgcca tgtgccagtt ggtgacctat tggctaacct
     6841 tgacgcgcct gccaaagaaa ggttgattca tgacgattga tccggattgc agaatactgc
     6901 tggtggaaga cgacgaaagc gatgcgcact tgttgcgcca gtcgttgctg cgggcggcca
     6961 gtttttcttt cgatatcgtc tgggtgaaga gcctggccga tgcgcggcga caattgagcg
     7021 aacatgcaca cgatgtcgtg ctgctcgatt tgaccctgcc ggattcgagc gggctggata
     7081 cggttcatgc cggtcgtttg gcggcggggg cgttgcctct catcgtgttg accggacatg
     7141 acgacaccga gttcgcattg caggcgcttg aggctggcgc tcaggattat ctggtcaaag
     7201 gcgattttca ggaaggcgag ctgatacgtg cgatacgcta tgcgatcagc cgggccaagc
     7261 ttgagcagcg tttgtacgag gcggaagagc gttggcgttt cgcgctggaa ggtgccggag
     7321 atggcgtgtg ggactggacc attcagactg acaaggtcgc gttttctccg cgactgaaag
     7381 agatgctctt cagcagcgag gctgaggtta aagagcatct tgacgaatgg aagcatcgcg
     7441 tgcatcccga tgatcaggcg cgcgtgatgg cggatttgca gacttatctg gagggtaaat
     7501 ctgccgccta tctgaatgaa catcgtctgc gctgcagcga cgacgcatgg aaatggatgt
     7561 tgcaccgcgg tatggtggtg agccgcactc cggagggtaa gccgctgcgc atgatcggca
     7621 cgttcaccga cattgaccgg cgcaagcggg cagaagaggc gctgcaaaga agtgaggcgc
     7681 tgttgaaaaa aacccagcag atcagtcagg tcggggggtg ggaatatgat gtggcgacac
     7741 agcatgtgcg ctggacggac gagacctatc gcatccatga attgacgtcc gatttcgatc
     7801 cgggcgaagt ttcggtcaac atcaagttct attctccctt ggatcaagtc gtgcttgacc
     7861 gtgctttcaa gaaggtcgtc gaacaggggg atgcatacga cctggagttg cagctgattg
     7921 gcgccaaagg aacccggaag tgggtcagaa cgacaggtca ggccgagcgt gtcgatggca
     7981 agattacgcg cgtcttcggc aacatcatgg atatcaccaa gagcaagcaa gctgaagaat
     8041 tgatgcggct ttccagcacg gtttttcaaa cggtcgatga agcgatcctg gtcagcgatg
     8101 tggataatcg catcatcacg gtcaatcccg ctttcaccaa ggtgaccgga tataccgcag
     8161 aagaagtgat cggtgaaaat ccgcgcatcc tgtcgagcgg gaagcacggt gaggcgtttt
     8221 accgcgtgct gtgggaccag ctcagtcgtg acggaagctg ggaaggggaa atgtggaacc
     8281 gccgcaagaa tggcgagatt tatgtcgaat ggacatccat caaacaagtg cgcgatgccc
     8341 acgggaagtt gacccactac gtggcggctt tttccgacat tacgacacgc aaggcaaacg
     8401 aggagagcat ccgccatcag gcgcagtacg atgcgctcac cgatctgccc aaccggatat
     8461 tgctattcga cagactggga caggcgatcg cgcaggccaa acgcgacaag actcaccttg
     8521 cgctgatgta catcgacctc gataaattca agccgattaa cgacaatctg ggccatgcga
     8581 tcggcgatca gttgctgcag gacgtggctt atcgtatgca ggattgtgtg cgtgcgatgg
     8641 atacggtggc acgtatcggc ggggatgaat tcgtcgtgct gctgccggtc gtggagaatg
     8701 agcaagacgc gttgagggtg gcggaaaaga tccgcgtcgc cctcaatcag ccgttcggga
     8761 tcgccggaga gagtttgagg atttcgtcca gcacgggcgt cgtcatttat cccgagcacg
     8821 gaaccagtga aaacgaactg accggcaatg ccgatatcgc catgtatttc gccaaggaaa
     8881 gcgggcggga taacgtgaag ttattcaaga aagcaatgcg ctcagcccaa tagcgatctg
     8941 gcttcggtgt gggcgaggac gcggctcgct tcgatattct tggtgtcctc gagtggaatc
     9001 gaaccactaa ttgaccctta ggaggggccg gttatatcca tttaactacg aggacgggac
     9061 gttttagaac gcaggggcgc attctagcgg aaatgcggcg caatcagaaa cctgttggct
     9121 ggtatcatgc gcgccacggt agaaattgtg aaaaggaaac tcgaaatgca ggctcgtatc
     9181 aatctatttt ttgcgtggtt ttttattcca cagacgctgg caatgggctg ggtagcggca
     9241 tccggccgca tcgtgctgga gctgctgggc accgacaccc gcgagggcga tattccagga
     9301 cgcatggtgg gcgcactgct gttgctgggg gcgatttatc tggtgctgca ttttcgcggc
     9361 tcgttgccac ccgaaggcaa gtcggacggt aacggctatc gtttcgggca tcgcgccgtg
     9421 ttgctgggga atgtgctggc tgcgctgctg ttcgtgttcc agtttgtcgc gccattgatc
     9481 gatagctaca acacccacct gattctggat aatttcacca ccgcctttgg atactgggcc
     9541 atggcctgtt gggtcgtggg tttctctttc ctctatcagt ctgcgcaact caagcagtcc
     9601 acaccgtccg agcaatctgc accgtctaga aagtagcctt cacccatgcc attcattact
     9661 ctggataaag ccagtctcgc cttcgggcac gtcgcattgc tcgatcatgc cgattttcaa
     9721 ctcgacgagg gggagcgcgt cggcctgatc gggcgcaatg gtggtggcaa gtcgagcatg
     9781 atgcgggtgc tggcaggtca gggcaacctc gacgacggac tggtgtggcg tgccccgacc
     9841 gcgcgcatct gctacgtcag tcaggagccc gtactggacg ccgatgactc ggtgttcgat
     9901 gcggtagcca aggggctggg caagctgcaa aagctgctgc acgattatca ccatgtctcg
     9961 caccagctct ccgaaccgga tgcggacttc gataagctgc tcgaggaaat gcaggtattg
    10021 cagacgcaac tcgaggcgca gaatggctgg tcggtgcagg cgcgcatcga gacggccatc
    10081 gccaagctgg atctggatcc cgagcggcgc gttggcgacc tgtccggcgg acagcgcaag
    10141 cgcgtagcac tggcgcaggc actggtggcc gagccggatg tgctgattct ggacgagccg
    10201 accaaccatc tggatttcgc ctccatcgaa tggctggaag gcctgctcaa cgacttcagg
    10261 ggatcggtgc tgttcgtcac ccacgaccgt cgctttctgg acaacgtgac cacgcgcatc
    10321 gtggaactgg atcgcggcaa gctggcgagc ttccccggca acttctcggc ctatcaggcg
    10381 ctcaaggagc gcatgctggc ggacgaggcg gtgatgaacg ccaagttcga caaggtgctg
    10441 gcgcaggaag aggtgtggat acgccagggg gtgaaggcac gccgtacccg caacgaaggc
    10501 cgtgtgctgc gtctggagca gttgcgccgc gagcgtgcag cgcgtcgcga caaggtcggc
    10561 aaggtcgaga tgaccgtgga gaccggcgag cgctcaggca agctggtggc cgagctgacc
    10621 gatgtgagca agtcgttcgg tggacgcaag atcgttgata acttctcctg ccgcattcag
    10681 cgcggggaca agatcggcct gctgggcccg aacggtgcgg gcaagagcac cttgctgaag
    10741 atgatcctcg gcgatctgga gccggacagc ggtaccgtca agctcggcac caagatatcg
    10801 gtggcctatt tcgaccagtt gcgcgagcag ctggacgagg aggcgacgct ggccgacacc
    10861 attagtcagg gcgctgattt tgtcgagatc ggcggtcaac ggaagcacgt catcagctat
    10921 ctgggggact ttctgttcgc gccggagcgc gcacgttcgc cggtgaagag ctgctcgggt
    10981 ggcgagcgca accgcctgct gctggcgcgg ctgttcaccc gcccggccaa cgtactggtg
    11041 ctggacgagc cgaccaacga cctcgacatc gagacgctgg aattgctgga agagctgctg
    11101 gcgaattacg acggcacgct gtttctggtc agtcatgatc gcgccttcct ggacaacgtt
    11161 gtgacacagg tgatcgcctt cgagggtgac ggcaagctga tggaatatgt cggcggttat
    11221 gaagactggg tgcgggtgca gaagtttcag gctgcacagc aggcgaccaa gcctgtcgcg
    11281 gcagttaagc aacccgtgca ggccgcacca gtcgagaagc cgaagcaaag caagctcagt
    11341 tacaaggacg cgcgcgaact ggaagagctg cccaagcgta tcgaggtgct ggagcaagag
    11401 cagatcgata tcgccgcgca cttcgccgac ggtagcctct tccgcgacga tcccaagcgc
    11461 gccaagcagt tgcaggcgcg cagcgaagag atcgaggcgg aagtgttggc cgcgatggcg
    11521 cgatgggaag agctggagca gcgctaagcg gaggtcgcgg tgcaccttgt tggtgcgccg
    11581 cgctgtccga ttacgggaat tgcgcaccaa tttgagtctt tgggggcggg tcgttcacta
    11641 cgcatgtgct cgcatccttt aattttcagt tacttggatt tttggcacac ttgttgcgta
    11701 tataggcgga tttgcaccaa aggagaaccc agtggaagag cttaaaacgg ccaatgatgt
    11761 tttatttgta ctgttaggtg ccattatggt gctggcgatg cacgccggtt ttgccttttt
    11821 ggaactgggc acggtgcgca agaaaaatca ggtcaatgca ctggtgaaga tcatcagtga
    11881 cttggctatc tcgaccctcg cttacttttt catcggctac ggtatcgcct acggtgtgag
    11941 ctttttcgtc ggtgcggaag agttggcgca gcgcaatggc tacgatctgg tgaagttctt
    12001 ttttctgatg acttttgctg cagcgattcc cgccatcgtt tccggcggca ttgccgagcg
    12061 ctctcgcttc aatccgcagc tggcggcgac cttcatgctg gtcggcttcg tctatccctt
    12121 cttcgagggt atcgcgtgga atcatcgctt tggcatacag gactggttgc atgccacttt
    12181 cggcgcagag tttcacgact tcgccggttc agtggtcgtg catgcggtag gcggctggat
    12241 cgcattggcg gccgtgttgc tgctcggtgc acgccgtggc cgctataaca aggaaggccg
    12301 catctccgcc catccgccct ccagcatccc gtttctggcg ctgggcgcgt ggatactgac
    12361 tgtcggctgg ttcggcttca acgtgatgag tgcacagaac atcagcggca tcagtgggct
    12421 ggttgcggtc aattcactga tggcgatggt cggtggcaca ctcaccgcac tgtggttcgg
    12481 caagaacgat ccgggcttcg tgcataacgg ccccttggct gggctggtcg cggtgtgcgc
    12541 cggttccgat ttgatgcacc cgattggtgc gttggtggtc ggtggcgttg cgggggcgtt
    12601 gttcgtggtg ttgttcacgc agacccagaa ccgctggaag atcgatgatg tgctgggggt
    12661 ctggccactg cacggtctgt gcggtgcttg gggcggcatc gctgcgggca tcttcggtgc
    12721 caaggcattg ggtggtatag gcggtgtcag cttgatgtcg cagctcgccg gcactttgct
    12781 gggcgtgagc attgccttca tcggcgggta tgtagtttac ggtgcgctca agaaactggt
    12841 cggcattcgc ctcgaaccgg aagatgaatt caacggtgcg gacttgagca tccacaagat
    12901 cagtgccacc ccggagcgtg aatcgggttg gtaagcatcc ctcttcgtcg ataggaaagg
    12961 gcaagccgta atggcttgcc ctttctgttt tgtggctgag taactttaat tcttcgtggc
    13021 ttcgatcggg tcaccgtgtg tgattcaatt cattcgatag tctgggtttc aatgcggatg
    13081 cgcaccactc gagcgagtgg tgcgcatggt gcttaagctg ccgccagcat cttgccgaac
    13141 agggcctcat gtgtctgctc gccgatgtcg aagtcgatcg caacggtcgt ttccacgccg
    13201 atacgcatag gcggcccgaa gcgggtgatc gcaaatcctg agcgtcgcat catgcgttcg
    13261 atggaaatgg tcgtgaccag gacatagcgt tcgattccca tctggtcgcc gaatctgacg
    13321 atttcgcgca atgcatcgag ggtcatgccg gagaatccga atcctcgcga gtcatccgac
    13381 ctgatagcga agcggcttaa ctcccatgtc ttcttgtctt ccggagcctt ctgtccatat
    13441 agtaattccg ggaaggtgtc cttcagcata tagggcccct cggttggcag gatgcgcagg
    13501 cagccccgca cgccatcgag cgtgtcgcga atcatcaggt aatgcgggtt gatggcatcg
    13561 taaccgtcga tttccatgcc gctcatgacg ggcacatccc agcccatctg ttccttgaaa
    13621 acctggttgc gcagcttgtg catctcccac aagtcttt
//
LOCUS       DASUIV010000049         6246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_941436, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000049 DASUIV010000000
VERSION     DASUIV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941436"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFW59_01640"
     CDS             <1..649
                     /locus_tag="VFW59_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5362947.1"
                     /translation="MDGYDVCRRLKSSAATVNIPVIFLTARLDEADEMKGLALGAIDY
                     ITKPINPNILLARVRNHLLLKKLVDFLLDQNEQDTAERIARSLKDISGEIGPEGLQQL
                     AEKLNLSIKSNRLHEEYRGQPAQPVLIAQSVQKIKAEQHKTIMVNQQKLKGVCDRLEE
                     LLRNDDAEALNVMDANAELLDIAYPDHSRKIDNSIRSFDFKAALVALRGATGTSA"
     gene            664..1821
                     /locus_tag="VFW59_01645"
     CDS             664..1821
                     /locus_tag="VFW59_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HEX5362948.1"
                     /translation="MDELETARRPTILVVDDTPDNLTLMSSLLKEHYKVKTANGGEKA
                     LKIAASTPPPDLILLDIMMPGMDGYEVCKRLKLNPATMNIPVIFLTAKSEVEDEKQGL
                     ELGAVDYITKPISPSIVMARLKNHLALKAMSDFLRDRNEFLELEVAKRTREVMAIQDV
                     TILAMGSLAETRDTETGNHIRRTQFYVKALAEKLRGHPRFAWFLTEANIKLLFKSAPL
                     HDLGKVGIPDRILLKPGRFEPHEFEIMKTHTTLGYEAIVHAEKALGFDVDFLSFAKTI
                     ALSHQEKWDGSGYPQGLTGDDIPIAARLMAVADVYDALISRRVYKDGMPHEQAVLIMI
                     DGRGTHFDADMFDAFIDIQEEFRAIAGRFVDSNVDMEKKKDYLSQAGATTP"
     gene            1818..3425
                     /locus_tag="VFW59_01650"
     CDS             1818..3425
                     /locus_tag="VFW59_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5362949.1"
                     /translation="MIDKQIAFLVVDDFEPMRKVTVGQLRTMGATSIATAGNGAEALR
                     MLQNRRIDIVLSDWNMPVMTGLELLKAVRSDPKLRHLPFIMITAEAERQRIEEAIACG
                     VSDLLVKPYTSQRLHTHVEKALTVQFPIRLPAGVGTISSRAAIAMVGSTPPTDHAEPE
                     HLTILVVDDMADNLLLLSQLLKDEYRVRIAHTGEKALEICQSNDPPDLVLLDIMMPGI
                     DGFEVARRMREHPTSEAIPVIFVTSMTDDDARLKAMGLGAVDFVTKPINPEILKPRVR
                     NFMRYVELRRQLQANYDNMLELARLREDVEHIMRHDIKGPLAGVVGLVQALAEDSSMN
                     YQQVWQLHMAEETTMQVIDMINLSSELFKIETGRFKLDAKRVKIADILRRIVEISRTT
                     FSGMNVSFSSEPDMAADSIAPDALGDAMLCYSLFQNLVKNACEAAPEGSRVDITLNDE
                     TPLRITIRNQGAVPAEIRERFFDKFVTHGKQGGTGLGTYSAKLLTEAQHGTIALDVSD
                     TEDQTTITVTLPRYLEVAATSVQKTSP"
     gene            3422..5023
                     /locus_tag="VFW59_01655"
     CDS             3422..5023
                     /locus_tag="VFW59_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5362950.1"
                     /translation="MTDTAKSPTILIVDDSPENLTLIGNLLKNKYQVRVASDGPNALR
                     IATSGDAPDLILLDVMMPGMDGYEVCKRLKSNPETMDIPVIFISARTEVEDETKGLEL
                     GAIDYIIKPVSPPIVLARVKNHLALREKSIELERARAVAEKANLAKSDFLSSMSHELR
                     SPLNAILGFAQLMGSDTPPPTASQKASIAHILQAGWHLLKLINEILDLAKVESGQIDM
                     SEEPVSLAEVMLECKNMIEPLAQLRGIKLSLPIFESPRFVRADRTRLKQVLINLISNA
                     IKYNSALGTITVSCTENSPGRIRISIKDSGAGLSAEQLTQLFQAFNRLGQEEGEEEGT
                     GIGLVVAKRLVEMMGGTIGAESTVGEGSVFWFELLAADEPRLEMPARETAPMSGARAP
                     IDSPAKTVLYVEDNPANMRLVEHILARHPHIRLLNAVNGNNGIEIARAVRPDAILMDI
                     NLPGISGLEALKILREDPTTMHIPVIAVSANAMPLAIERGLEAGFFRYVTKPIQIDTF
                     MDALNVALESAQAVPEPTNPVTDFD"
     gene            5016..5984
                     /locus_tag="VFW59_01660"
     CDS             5016..5984
                     /locus_tag="VFW59_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5362951.1"
                     /translation="MISSSDILNAGILIVDDEEPNVILLERMLHGAGYLSVSSTMNAQ
                     KVGELYRKNRYDLILLDIRMPGMDGFKVIEALKKIETDDFPAVLVITAEPGHKERALQ
                     AGAKDFVSKPFELVEVLTRVHNLLEARLLQKEIADHKEDNKALSNVIQRNIRKIILVR
                     KKAIREQGSQDRLANAMTSFAGSMAFFYVHIAWFLIWFVLNTGHLGITPFDPYPYGFL
                     TMVVSLEAIFLATFVLISQNLLARETERLTDLGLHTGLLTEHELTRVLQMLSAIQDKI
                     GISNEANRDLADADLEMETNLEDVLAEIERLQQRDKAATLHGRKSG"
BASE COUNT         1505 a         1818 c         1746 g         1177 t
ORIGIN      
        1 catggacgga tacgatgtct gccgacgact caagtcatcc gctgcaaccg tgaatattcc
       61 ggtgattttt cttaccgccc ggcttgacga ggcggacgag atgaaaggac tggcactggg
      121 cgccatcgac tacatcacca agccgatcaa tccgaacatt cttctggctc gcgtaagaaa
      181 ccatctgctt ctaaaaaaac tggtcgactt cctgctggat cagaacgagc aggatacggc
      241 cgaacggatc gcccgctcgc tcaaggatat ttccggcgaa atcggccccg agggtcttca
      301 gcaattggct gaaaaactca acctgtcgat caaatcgaac cggttgcacg aggaatacag
      361 gggacagccc gcccagcctg ttctcattgc gcagagtgtt cagaaaataa aagcagaaca
      421 gcacaaaacc atcatggtta atcagcaaaa gctcaagggg gtatgcgatc gcctggagga
      481 attgctccgc aatgatgatg cggaagccct taacgtgatg gacgccaatg cggagctgct
      541 tgatatcgcc tatcccgacc attcccgcaa gatagacaac agcatccggt ctttcgattt
      601 caaggcggct ctcgttgcat tgagaggcgc gaccggaaca tcggcctgac aaggagcgct
      661 cccatggatg aactcgagac agctagaaga ccgacgatcc tggtggtgga tgacaccccg
      721 gacaacctga cgctgatgag cagcctgctc aaggagcatt acaaggtaaa aacggccaac
      781 ggcggagaga aggcgctgaa gatcgcggcc tcgacgcccc cgccggatct gatcttgctc
      841 gacatcatga tgcctggcat ggatggctac gaggtgtgca aacgactcaa actcaatccc
      901 gcgacgatga atattccggt catttttctc accgccaaga gtgaagtcga ggacgaaaaa
      961 caaggactgg agctcggcgc ggtcgattac atcaccaagc ccatcagccc ctccatcgtg
     1021 atggcgcgcc tgaagaacca tcttgcactc aaggcgatgt ccgacttcct gcgcgatcgg
     1081 aacgagttcc ttgaactcga ggtggccaag cgcacgcgcg aagtcatggc gattcaggat
     1141 gtcaccattc tcgccatggg ctcgctggcc gagacgcggg atacggaaac cggcaaccat
     1201 atccgccgca cacagttcta cgtcaaggcg ctggccgaaa agttgcgggg tcatccgcgc
     1261 tttgcctggt tcctgacgga agccaacatc aagctgttat tcaagtcggc accgttgcat
     1321 gacctcggca aggtgggcat cccggaccgc atcctgctca agccgggccg cttcgaaccc
     1381 catgaattcg agatcatgaa gacgcatacg acactgggat acgaggcgat cgtgcatgcc
     1441 gaaaaggcgc tgggtttcga tgtcgatttc ctctcattcg ccaagacgat cgccctgtcg
     1501 catcaggaaa aatgggatgg cagcggctac ccgcagggac tgaccggcga cgacatcccg
     1561 atcgcggcgc gcctgatggc ggttgccgat gtctacgacg cgctgatcag tcgtcgcgta
     1621 tacaaggacg gcatgccgca tgagcaagca gtgctgatca tgatcgatgg caggggcacg
     1681 catttcgacg ccgacatgtt cgatgccttc atcgacatcc aggaagagtt tcgtgccatc
     1741 gccgggcgtt ttgtcgattc aaatgtggat atggagaaaa agaaagacta cctgtctcag
     1801 gccggtgcga cgacaccatg atcgacaagc agatcgcctt tctcgtcgtc gacgatttcg
     1861 agccgatgcg caaagtgacg gtggggcaac tgcgcaccat gggcgccacc agcatcgcga
     1921 cggcgggaaa cggtgcggag gccctgcgca tgctgcaaaa ccgccggatt gatatcgtgc
     1981 tttccgactg gaatatgccg gtcatgacgg ggctggaact actcaaagcg gtgcgttccg
     2041 atccaaaact ccggcacctg cccttcatca tgatcaccgc cgaagcagaa cgccagcgta
     2101 tcgaggaagc cattgcctgc ggcgtcagcg atctgctggt caaaccctac acttcgcaac
     2161 gtcttcatac ccatgtggag aaagcgctga ccgtacagtt cccgatcaga cttcctgctg
     2221 gagtgggaac catctcaagt cgcgcggcga tcgcgatggt tggctccacg ccgccgacag
     2281 accacgcaga gccagaacac ctgacgattc tggttgtcga cgacatggca gacaatctgt
     2341 tgctgctctc gcagctactc aaggacgaat atcgcgtgcg catcgcgcat accggcgaga
     2401 aagcgctgga gatatgccag tcaaacgatc cgcccgatct ggttctgctc gatatcatga
     2461 tgccgggcat agacggcttc gaagtcgccc ggcgcatgcg cgaacacccg acctccgaag
     2521 cgattccggt gatcttcgtc acctcgatga cggatgacga tgcgcggctg aaggccatgg
     2581 ggctgggtgc ggttgatttc gtcaccaaac ccatcaaccc ggagatactc aaaccgaggg
     2641 tgcgtaactt tatgcgctac gtcgaattgc gccggcaact gcaggccaac tacgacaaca
     2701 tgctggagct ggcacgactg cgcgaggatg tcgaacacat catgcgccat gacatcaaag
     2761 gaccgctggc cggcgtggtt ggactggtgc aggcgctggc ggaagacagc tcgatgaact
     2821 accagcaagt ctggcagttg cacatggccg aagaaaccac gatgcaggtg atcgacatga
     2881 tcaatctgtc ttccgaactt ttcaagatcg aaaccggacg cttcaaactc gatgccaagc
     2941 gggtcaaaat agccgacatc ctgcgccgca ttgtcgaaat ttcacgcacg actttctccg
     3001 ggatgaatgt ctccttctcc agcgagcctg acatggcggc cgacagcatc gctccggatg
     3061 cactgggcga cgccatgctc tgctattccc tgttccagaa tctcgtcaag aatgcctgcg
     3121 aagcggcgcc agaaggcagc cgcgtggata tcacgctgaa tgacgaaacg ccgttgcgca
     3181 tcacgatccg gaatcaggga gcggtaccgg cagaaatacg cgagcgattc ttcgacaagt
     3241 tcgtcacgca cggcaagcag ggcgggaccg gcctgggaac ctattcggcc aaattgctca
     3301 ccgaggcgca acacggaacg atcgcgctgg acgtttccga caccgaggat cagaccacga
     3361 tcaccgtaac gctgccgcgt tacctggaag tcgccgcgac atccgtccaa aagacatcac
     3421 catgaccgat actgcaaaat cccccacgat actgatcgtc gacgacagcc cggagaacct
     3481 gacgctgatc ggcaatctgc tcaagaacaa ataccaggtg agggtcgcca gcgacgggcc
     3541 gaatgcgctg aggatagcga catccggcga tgcgccagac ttgattctgc tcgatgtcat
     3601 gatgcccggc atggacggct acgaggtatg caaacggctc aaaagcaacc ccgaaacgat
     3661 ggacatcccg gtgatcttca tttccgcaag aaccgaagtc gaggacgaaa ctaaaggact
     3721 ggaactgggc gcaatcgact acatcatcaa accggtcagt ccgcccatcg tgctggcacg
     3781 cgtgaagaat catcttgccc tgcgggaaaa gagcatcgag cttgaacgcg cccgcgcggt
     3841 ggcggaaaag gccaatctgg caaaatctga cttcctctcc agcatgagtc atgagctgcg
     3901 cagtccgctc aatgccattc tcggcttcgc ccagctgatg ggttccgata cgcccccccc
     3961 gacggcaagc cagaaagcca gcattgccca tattcttcag gcgggctggc atctgttgaa
     4021 actgattaac gaaatcctcg atcttgccaa agtcgagtct ggacagatcg atatgtctga
     4081 ggaaccggtg tcgctcgccg aagtgatgct cgaatgcaag aacatgatcg aaccgctggc
     4141 acaactgcgc gggatcaaat tgagcctgcc catctttgag tcaccccgct ttgtccgggc
     4201 ggatcgaacc cggttgaaac aggtattgat caacctgatc tcgaacgcga tcaaatacaa
     4261 ctcggcgctg ggaacgataa cggtgagctg cacggagaac tcgccgggac gcatccggat
     4321 cagcatcaaa gacagcggcg cgggcttatc tgccgaacaa ttgacgcagc tctttcaggc
     4381 attcaaccgg ctggggcagg aggaaggaga ggaggaagga acgggcatcg gactggtggt
     4441 ggccaaacga ctggtcgaaa tgatgggggg caccatcggc gcggaaagca ccgtcggcga
     4501 aggcagcgtg ttctggttcg aactccttgc ggccgacgaa ccgcgtcttg aaatgcctgc
     4561 aagagaaacg gctcccatga gcggtgctcg cgcgccgatt gattcccccg ccaaaaccgt
     4621 gctttatgtg gaagacaatc cggcgaatat gcggctggtc gagcatatcc tcgcccgtca
     4681 cccgcacatc cggctgctca atgcggtgaa tggcaataac ggtatcgaga tcgcgcgggc
     4741 cgtcaggccg gatgcgattc tgatggacat caacctgccg ggcatcagcg gcctcgaagc
     4801 gctgaaaata ttgcgcgaag acccgaccac gatgcatatt cccgtcatcg cggtcagcgc
     4861 caatgcgatg ccacttgcga tagaacgggg cctggaagca ggattcttcc gctatgtgac
     4921 caagcccata cagatcgata cattcatgga tgcgctcaat gtggcgctgg agtctgcgca
     4981 ggccgttccg gaaccaacga atccggtaac cgactttgat tagctcatcc gacatcctga
     5041 acgcgggcat cctgatcgtc gacgacgagg agcccaatgt gattctgctg gagcgcatgc
     5101 tgcacggcgc cggttacctt tccgtctcat ccaccatgaa cgcacagaag gtcggcgagc
     5161 tctaccgcaa gaaccgctat gacctgatcc tgctggatat caggatgccc ggcatggacg
     5221 gattcaaggt gatagaagcc ctgaaaaaaa tcgagacaga cgattttcct gcggtactgg
     5281 ttatcaccgc cgaacccggc cacaaggaac gtgcgttgca agccggcgcg aaagacttcg
     5341 tcagcaagcc ctttgaactg gtcgaggtgc tgacccgggt tcacaacctg ctggaagccc
     5401 gcctgctcca gaaagagatt gccgaccaca aggaagacaa caaggcgctg tccaacgtca
     5461 tccagcgcaa cattcgcaag atcatcctcg tgcgcaaaaa agccatccgc gaacagggat
     5521 cacaggatcg cctcgccaat gccatgactt catttgcggg cagcatggcc ttcttttatg
     5581 tgcatattgc gtggtttctt atctggttcg tgctcaatac cggccatctc ggcatcacgc
     5641 cattcgaccc ctacccttac ggattcctga ccatggttgt gtcgctggaa gccatattcc
     5701 tggcgacatt cgtactgatc agccagaacc tgctggccag ggagaccgag cgcctgaccg
     5761 atctgggctt gcataccggc ctgctgaccg agcatgagct gaccagagtg ctgcaaatgc
     5821 tgagcgccat tcaagacaag atcggcatca gcaatgaagc gaaccgcgac cttgccgatg
     5881 ccgacctgga aatggaaacc aatctggaag atgtactggc cgaaatcgag cgtcttcaac
     5941 aacgcgacaa ggccgccacc ctgcacggca gaaaatcggg gtagcgcggg gatatcctta
     6001 ttactcgtca ttcgggcccc tttcgtcccg aagcctgctt taaccgtttc agctgccgag
     6061 ctaaagatgg cagagcaagg tggcatgacg atgcccgatc ggtttactgg aaacgcaact
     6121 cgaattcgcc tgaaccgcgt tccatcccga tcaggcgcaa cagcggattg agttgctgcc
     6181 ggtgctctgg tgggacacgg gcgatggcga taaattcggg tctgtcccca ttcatccatg
     6241 cgccat
//
LOCUS       DASUIV010000050         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_12602, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000050 DASUIV010000000
VERSION     DASUIV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12602"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VFW59_01665"
     CDS             <1..1126
                     /locus_tag="VFW59_01665"
                     /inference="COORDINATES: protein motif:HMM:NF039793.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362952.1"
                     /translation="HPFPPLGGIHISTRRGSESVKNDFLARLEQLGLDRYVMSQDRAG
                     ADGEIVIFQLREHIADDLTPNHDTTRLCQKIDLDTGNDPLDLEKEILLAMLASPAPFP
                     FPSHAELSSSVRMRKNIVAAARKTLLSFDTCEAERPESHWTYVADCGFTLLPKKDLIT
                     ALTKATQPDESGRRYSFSCYRASEYIILLGIAQELKSCNPELLDRLQDQCGKRAILSG
                     EFHEVFLREYGSMSEPLPPKYWVPGDRTWFRNPDDHSSDVTGYEGSWVIYLGDGLFSN
                     FWKENEPYSLTTKCIEIFHWRNATYRDQAGELKIDEAVVEQRVRSSLQDPAEVERILD
                     IMLQLREPQGVYVDGGCIDTSREYPRWVCSGTSDLALPEQ"
     gene            complement(1203..3395)
                     /locus_tag="VFW59_01670"
     CDS             complement(1203..3395)
                     /locus_tag="VFW59_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5362953.1"
                     /translation="MKLPKPEAGDFWGGLAAMLVALPSAIAFGVTIYSPLGSSFAAQG
                     ALAGILGVVALGLVAPAMGGTNRLISAPCAPAAAVLSAFAIEFMRGGGSAGAALLLIS
                     LLGLLAGLLQMLFGTIRLGQLIKYMPYPVVSGYLSGVGLIIIGSQVPIFLGTAKGTHF
                     WASLAVPERWQWQSMVVGAVTIAVMLTASRVTRAVPAAILALLAGMLAYFGLGLADPT
                     LLALEGNPFVVGPLGGSGGSFTDAMTDRWRAMGDVGIDQIRSIAVPALTLAVLLSIDT
                     LKTCMVLDALTHSRHNSNRELIGQGLGNVASAMIGGIAGAGQMGATLVNISSGAVSRL
                     SGVFEGVLALVAFLVLGALISWVPVAGLAAILIVVGVRMIDRHSLQFLKQRATMLDFA
                     VIAAVVATALTVSLIAASGTGIVLAVVLFIREQIGGTVVRRKLSCSEVFSKRVRPQGE
                     MEILVARGGRGVVVELQGSLFFGTTSQLYSALEPELKTRDYLILDMRRVQSVDVTAAH
                     MLDQAKDMLAERQGFLVLSQLPQNLPSGQDMQQYFDQVGLVRPENPVRVFGELDEALE
                     WVENRILDDVICELGEQEALNLNEIELFKGRKAETLAALESCMEKRSYRAGEKIFQLG
                     DTGDELFLIRKGAVRIMLPISERQTHHLGTFGRGAFFGEMAFLDGDMRSADAVAFSDT
                     DLYVLSRKTFDSLAEEHKKLALGLMEGLASVLASRLRYTNAELRVLES"
     gene            complement(3730..3806)
                     /locus_tag="VFW59_01675"
     tRNA            complement(3730..3806)
                     /locus_tag="VFW59_01675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3770..3772),aa:Arg,seq:ccg)
     gene            complement(3926..>5011)
                     /locus_tag="VFW59_01680"
     CDS             complement(3926..>5011)
                     /locus_tag="VFW59_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX5362954.1"
                     /translation="LFLGTGAAMLVGADKIASIDAGGNMAGPLLAQFLGGGEQSYMGN
                     FMLAFVAAVAFATIVAVVAGLVLAAASAMAHDIYVGVIRGEKATPKEQVVAARVSSII
                     VGVIAITVGILAKGQNVAHLVALAFAVAASSNLPAVFLTLYWKKCNTTGIILGMVVGS
                     LTAIGLVMVSPNMTYPKAVIAGANKVLNGEPAQPAKEAVPAVAASSFVCELFALDGCV
                     KSEPAKAASPEKPAKPGAAEKLAGLNVKLQSLEASLATVADPAAKTAAEADLVKVKKD
                     IAATEKAKAKAEDDLKKFEGQTTSIMGLEKPFFLLKNPGLISIPLGFLLVILGSLFTR
                     DKRAEEMWDELYVRQNTGINAEAASAH"
BASE COUNT         1097 a         1597 c         1428 g          889 t
ORIGIN      
        1 ccacccattt ccgccactcg gtggcatcca tatttcaacc agacggggaa gtgagtcggt
       61 gaagaacgat tttctggccc ggcttgaaca actcggtctt gaccggtatg tcatgtcgca
      121 agacagggcc ggagcggatg gcgaaatcgt catctttcag ctgcgcgagc atatcgccga
      181 cgacttgaca cccaaccacg atacgacacg gctctgccag aagattgatc tcgacaccgg
      241 aaacgatccc ctcgatctcg aaaaggaaat cctgcttgcc atgcttgcaa gtccggctcc
      301 cttcccgttt ccgagtcatg ccgaactatc atcgtctgtg cgcatgagga aaaacatcgt
      361 ggctgcggca cgcaagaccc tgctgtcttt tgacacctgc gaggcggagc gccccgagtc
      421 tcactggacc tatgtcgccg actgcggatt caccctgctg cccaagaagg atctgatcac
      481 ggcccttacc aaggcgactc agcccgacga atcaggcagg cgctattcct tctcctgtta
      541 ccgcgcgtcc gagtacatca ttctgctcgg catcgcacag gagctgaaga gctgcaaccc
      601 ggaactgctg gacaggctgc aggatcaatg cggaaagcgc gccattctgt cgggcgaatt
      661 ccacgaagtg ttcctgcgcg agtacggctc catgagcgag ccattaccgc cgaaatactg
      721 ggtgccgggt gatcgcacct ggttccgcaa cccggacgat cattcttcag acgtgacagg
      781 ctatgagggc tcctgggtga tctacctcgg cgacggtctg tttagcaact tctggaaaga
      841 gaatgagcct tactcgctga caacgaagtg catcgagatt tttcattggc ggaatgcgac
      901 gtaccgggat caggccggcg aactgaaaat cgacgaggcc gtcgttgaac agcgggtacg
      961 atcttcgctg caagacccgg ccgaggtcga gcgcatcctc gatatcatgt tgcagttacg
     1021 cgaaccacaa ggcgtctatg tcgatggcgg ctgcatcgac acctcgcgcg agtatccccg
     1081 ctgggtgtgt tctgggacat ccgatctggc gctgcccgag cagtgaaggc attccgaaga
     1141 tccgcacaca gctctcgcag actcggcgta tgcacatcgg caggtgcgcg cccagacagg
     1201 aatcaactct ccaacacacg caattccgcg ttcgtataac gcaagcggct ggcgagcacg
     1261 ctagccagcc cctccatcag acccagcgcg agcttcttgt gctcttcggc cagcgagtcg
     1321 aaagtcttgc gcgaaagcac ataaagatcg gtatcggaaa acgccacggc gtcggcggaa
     1381 cgcatatcgc catcgagaaa agccatttcg ccaaagaaag cgccccggcc gaatgtgccc
     1441 agatgatgag tctgccgctc ggagatcggg agcatgatgc gcaccgcccc cttgcgaatc
     1501 aggaacagtt catccccggt atcgccaagc tggaagatct tctcacccgc ccgataggaa
     1561 cgtttttcca tgcacgactc cagcgcggca agcgtttccg ccttgcgccc cttgaacaac
     1621 tcgatctcat tgagattgag cgcctcttgt tcgccgagct cgcagatcac atcatcgagg
     1681 atgcggttct cgacccattc aagcgcctcg tcgagttcgc cgaacacgcg caccggattc
     1741 tccgggcgca ccagaccgac ctgatcgaaa tactgctgca tatcctgccc cgaaggcagg
     1801 ttctgaggaa gctggcttaa aacgaggaaa ccctggcgct cggcgagcat gtccttggcc
     1861 tgatccagca tgtgggcggc ggtgacatcg acggactgca cgcgacgcat gtcgagaatg
     1921 aggtaatccc gcgttttcaa ctccggctcc agcgcagaat aaagctgact ggtggtgccg
     1981 aagaacaggc tgccctggag ctcgacgacg acaccgcgcc cgccgcgcgc gacaagaatc
     2041 tccatctccc cttgaggtcg cacccgcttg gaaaacacct cgctgcatga aagcttgcgg
     2101 cgcaccacgg taccgccgat ctgctcgcgg atgaacagca ccacagccag cacgataccg
     2161 gtgcccgatg ccgcaatcag gctcacggtc aatgcggtag cgacgacggc agcgatgacg
     2221 gcgaagtcga gcatcgtggc gcgctgcttg aggaactgga ggctgtggcg gtcgatcatg
     2281 cgcacaccga cgacaatcag gatcgcggcg agtcccgcca ccggaaccca ggatatcagc
     2341 gcacccagca ccaggaaggc gaccagcgcc agcacccctt caaagacacc cgacaaccgg
     2401 ctcaccgcac cgctggagat gttgaccagc gtcgccccca tctgacccgc cccggcaata
     2461 ccgcctatca tcgccgaagc gacattgccg aggccctggc cgatcagctc gcggtttgaa
     2521 ttgtggcgcg agtgggtcag cgcatccagc accatgcagg tcttcaacgt atcgatggaa
     2581 agcagcaccg ccagcgtcag cgcggggacg gcaatgctcc tgatctggtc gatgccgaca
     2641 tcacccatgg cgcgccagcg atccgtcatg gcatcggtga aactgccgcc gctgccgccg
     2701 agcggaccga cgacgaaggg attgccttcc agcgcaagca gcgtcggatc cgccagaccc
     2761 agcccgaaat aggcgagcat gcccgccaac agcgcgagta tcgcggcggg caccgccctt
     2821 gtgacccgtg acgcggtgag catcacggct atggtcaccg caccgacgac catgctctgc
     2881 cattgccacc gctccggcac tgcaagcgac gcccagaaat gggtaccttt ggccgtaccg
     2941 aggaagatgg gcacctggct gccgataatg atgaggccca cgccggacag ataaccgctc
     3001 accaccggat agggcatata tttgatcaac tgcccgagac ggatcgtgcc gaagagcatc
     3061 tgcaacaagc ctgccagaag ccccagcagc gagatcagca gcaacgcggc cccggcactc
     3121 cctccgccac gcatgaactc gatggcaaag gcagacagaa ccgccgctgc gggagcgcaa
     3181 ggagcggaga tgagccgatt ggtgcccccc atcgccggcg cgacaagacc gagcgcgacg
     3241 acacccaata tccccgccag cgcaccctgt gccgcgaaag aactaccgag cggagaatag
     3301 atcgtgacac cgaaggcgat agccgagggc agggccacca gcatcgcggc cagcccgccc
     3361 cagaaatccc ccgcttcagg cttaggcagc ttcatggctc agcccccgat aaacagttgc
     3421 cttatcctaa actcatatac cggcttacac aacgcgaact gagcgcgagt tgacgaaccg
     3481 tcaagatagg agtccaaaac ggcacgggtg ggactatccg agacgcggct ccggccgcat
     3541 ttctcaaagc ctcctggcat gcttgcacgc aaaggaggaa atcgagcctg tccgactttt
     3601 gcattgcgca aatttccgcc tcgactcgtt ttcaatatcg cacaagtgtg cgtcaaaaat
     3661 caccggggat ttatctgacc ggatagaaaa aaggcttact tcccttgaga aataagcctt
     3721 ttctggatgt ggtgcgctcg gcgggagtcg aacccacgac ccttggcttc ggaaaccaat
     3781 actctatcca gctgagctac gagcgcatat gcggcgcgca gtatagcctt tttaccccgc
     3841 gccgcgcgac tggaatgctt gatcggcgcg cataaaaaaa ccccgccggt aagggcgggg
     3901 ttgataaggt acagccgatg ctgcgttagt gagcggaagc cgcctcggcg ttgatgccgg
     3961 tgttctggcg tacatagagc tcgtcccaca tctcttccgc acgcttgtcg cgggtgaaca
     4021 acgaacccag gatcaccagc aggaatccca gcgggatcga gatcaggccg gggttcttca
     4081 acaggaagaa gggtttttcc agacccatga tgctggtggt ctgaccttcg aacttcttca
     4141 ggtcatcctc cgccttggcc ttggccttct cggtggcagc gatgtccttc ttcaccttga
     4201 cgagatcggc ttcagccgct gttttagcag ccggatcggc cactgttgcc agcgaggctt
     4261 ccagcgattg cagcttcaca ttcaaaccgg ccagcttttc ggcagcgccg ggtttcgccg
     4321 gtttttccgg cgaggcggcc ttggcgggct cggacttgac gcagccgtcg agcgcgaaca
     4381 attcgcacac gaagctggaa gccgcgactg cgggcaccgc ctctttcgcg ggttgggcag
     4441 gctcgccgtt caggacttta ttcgcaccgg cgatcaccgc tttcggataa gtcatgttgg
     4501 gcgaaaccat caccagaccg atggcggtca atgaaccgac caccatgccg aggatgatgc
     4561 cggtggtgtt gcacttcttc cagtacagcg tcaggaacac cgcaggcagg ttgctcgatg
     4621 ccgcgaccgc gaaggccagc gccaccagat gcgcaacgtt ctgacccttg gccagaatgc
     4681 ccacggtgat ggcgatcacg ccgacgatga tggaggaaac gcgcgctgca accacctgct
     4741 ccttcggtgt ggctttttcg ccacggatca cgccgacgta gatgtcatgg gccatcgcgg
     4801 aggctgccgc cagcaccaga ccggcgacca ccgccacgat ggtggcgaat gcgaccgccg
     4861 ccacgaacgc cagcatgaag ttacccatgt acgattgctc gccgccgccc aggaactgcg
     4921 ccagcaacgg gcctgccatg ttgccgccgg catcgatgga ggcgatcttg tcggcaccga
     4981 ccagcatcgc agcaccggta ccgaggaaca g
//
LOCUS       DASUIV010000051         6821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1221657, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000051 DASUIV010000000
VERSION     DASUIV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221657"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFW59_01685"
     CDS             <1..567
                     /locus_tag="VFW59_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5362955.1"
                     /translation="WHCAIHAYVLMTNHVHLLLQPGEAVADLGCFMKALSARTTRYFN
                     RQEGRSGTLWESRYKSSPVQTEQYLLACCRYIELNPVRARMVGHPGEYRWSSYTARMG
                     GVGHEWLDPDPMYQALGDDETTRRSRYLKFLEEAIPSGEWELIRESVKRGQLTGNTRF
                     AEAVEQIIGRRIARRGPGRPPKDEPEVG"
     gene            678..938
                     /locus_tag="VFW59_01690"
     CDS             678..938
                     /locus_tag="VFW59_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5362956.1"
                     /translation="MEAVTYSHTRQHLAEIMNKVGDDRAPVLITRQSGNPVVMMSLQD
                     FNALEETAYLMRSPKNARRLMESIEQLAMQGGQQRELLPDAD"
     gene            928..1182
                     /locus_tag="VFW59_01695"
     CDS             928..1182
                     /locus_tag="VFW59_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEX5362957.1"
                     /translation="MPIKFSEQAWEDYLFWQAKDRSTLKRINTLLRDVQRSPFEGIGK
                     PEPLKYNLAGFWSRRIDEEHRLVYAVQDDTILVAQCRYHY"
     gene            1334..2152
                     /locus_tag="VFW59_01700"
     CDS             1334..2152
                     /locus_tag="VFW59_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5362958.1"
                     /translation="MDHTVENLVEVKDLHFAYGPHKVLKGINLTIPRGKIVAILGSSG
                     CGKTTLLRHFGGQLRPSSGSVKFMGQEVHELGNDELYKMRLKMGMMFQVSGLFTDLSV
                     YDNLAFPMREHTDLSEEMIRDLVLMKLNAVGLRNARNLKPSELSGGMERRVALARAIA
                     TDPALIIYDEPFAGLDPISLNTIANLIRRLNDALGVTSVVVTYDLSESLKIVDYVYFI
                     HDGVVVAEGEASEMLNSQDPFVRQFVHAEPDGPVAFQYPSEPYAQELNISIAAA"
     gene            complement(2248..3888)
                     /gene="pgi"
                     /locus_tag="VFW59_01705"
     CDS             complement(2248..3888)
                     /gene="pgi"
                     /locus_tag="VFW59_01705"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX5362959.1"
                     /translation="MSKLTQSPAWQGLKAHHQVIGPLSMRQMFRDDPARFERFSVRLD
                     DLLFDYSKNRITAETMSLLLALAEQAALPAHVEQMFGGAKINATEHRAALHTALRNRS
                     DRPVLVDGRDVMPDVRRVLGLMRRFSEAVRNGEHRGHSGKQITDIVNIGIGGSDLGPL
                     MVCEALKPYGRANLRAHFVSNVDGTHLAETLKKLDAETTLFVVSSKTFTTQETMTNAR
                     SARAWLVDKLGDETAVAEHFAAVSTNLDATAQFGINPHNVFEFWDWVGGRYSLWSAIG
                     LPIALFIGMDKFEALLDGAYAMDEHFRHAPPDQNIPVLMGMLGIWYGNFFGAGANAVF
                     PYDQYLHRFPAYLQQLDMESNGKRVDRDGNPVDYDTGMVVWGEPGTNGQHAFYQLIHQ
                     GTRMIPADFLAPMRSQNPIGEHHAILLANCFAQTEALMVGKNEMEARAELEAQGLQGE
                     ALEALLPHKVFPGNKPTNTLLFDRLDPKTLGKLIALYEHKVFVQSVVWNINPFDQWGV
                     ELGKQLAGKILPELRGEGSASPHDASTSGLISHYQKQH"
     gene            complement(3914..6076)
                     /gene="glgB"
                     /locus_tag="VFW59_01710"
     CDS             complement(3914..6076)
                     /gene="glgB"
                     /locus_tag="VFW59_01710"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX5362960.1"
                     /translation="MNTPFDLLLQARHYDPFGLLGLHREGDEWVIRVYQPHATGVELL
                     NGTCNTALKAIHPGGVFEWRDKTEPSRPYRLRMHYGSATHETFDPYQFPPHLSPSDLY
                     LFSEGRLHQSYQMLGSHSVEIDGVKGVRFAVWAPNAERVSVVGEFNGWDGRVHPMRSH
                     GSNGVWELFLPEISSHALYRYEIRNRATGNILTKTDPYAQGYELRPGTAALAAPPNQH
                     RWQDAAWLKQRGEWDWLHGAINIYEVHAGSWKRHPDGRFYSYRELATDLVPYVQSMGY
                     THIELMPVSEHPLDESWGYQATGYFAPTSRFGSPDDLRHLIDTCHQADIGVILDWVPA
                     HFPQDNWALARFDGTALFEHEDPRLGFHQDWGTHIFNYGRNEVKSFLMSSAHYWLSEF
                     HFDGLRVDAVASMLYLDYSRKAGEWIPNRYGGRENLDAVDFLREMNIMVHEEFPGALT
                     MAEESTAWPGVSRPTYLGGLGFSIKWNMGWMNDTLSYFHHDPVHRRYHHDQLTFGQIY
                     AYSENFVLPFSHDEVVHGKGSLLSKMPGDAWQKFANLRLLMAFQMTWPGKKLNFMGNE
                     IAQGREWQSKWELEWWQLGQEFHRGVQNLTHDLNELYRQLPALHDIDFQQDGFSWLDC
                     NDAEKSVLSYLRHARDGSSAVIALNLTPIPREHYRIGLPKQASYREVLNSDSQFYAGS
                     NMGNAGLIHAEPIAWMGQPCSAVISLPPLAGVILVPEA"
     gene            6166..>6821
                     /locus_tag="VFW59_01715"
     CDS             6166..>6821
                     /locus_tag="VFW59_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5362961.1"
                     /translation="MNAELGYLQKKYHLAAGDTSPRFISRITKNTYAMVLAGGRGSRL
                     HQLTDWRAKPAVPFGGKFRIVDFVLSNCVNSGIRRIGVATQYKSHSLIRHIQRGWSFL
                     NGQFGEFLDILPAQQRVDEEHWYQGTADAVYQNLDILREHNPEFVLILAGDHVYKMDY
                     GKLLAFHVATKADMSVACLEVPIADASAFGVMGVDEHGRVVQFDEKPANPATIPGKPD
                     "
BASE COUNT         1428 a         1876 c         2084 g         1433 t
ORIGIN      
        1 tggcactgcg ccatccatgc ctacgtgttg atgactaacc acgtgcacct gctattgcag
       61 cctggcgaag cagtggcaga tttggggtgc ttcatgaaag cgctatcggc gcgcactacg
      121 cgttacttca atcggcagga aggccgcagt ggcacgctgt gggagagccg ctataaatcc
      181 agccccgtgc aaacagagca atatctgctg gcctgctgtc gttatatcga acttaaccct
      241 gtccgtgcgc ggatggttgg tcatcccggc gaataccgct ggtcaagcta taccgcacgg
      301 atggggggag tggggcacga atggctagac cccgatccga tgtatcaggc gctgggcgac
      361 gacgaaacaa ctcgacggag caggtacctg aagtttctcg aagaagcgat tccatccggc
      421 gaatgggagt tgatccgcga atccgtgaag cgcggccaat tgacagggaa tactcgattt
      481 gccgaggcgg tcgagcagat catcggtcgc cgaatagcgc gtcgcggacc gggcagaccg
      541 cccaaggatg agcctgaggt aggatgataa aagtgagcgg ggaagtggaa aaataaatct
      601 gtcccctttt ttggaatctg gagttgaatt tgctggctca ttgtactatt atttgtacaa
      661 atccgtagga gagggacatg gaagcagtta catacagcca cacccgccag catctggcag
      721 agatcatgaa caaggttgga gatgatcgtg ccccggtgct gatcactcgc cagtccggaa
      781 accctgttgt catgatgtca cttcaagact tcaatgcact ggaggaaacg gcatatttga
      841 tgcgtagccc gaagaatgcc agacgactca tggagtcaat cgagcagcta gcgatgcaag
      901 gcgggcagca aagagaactg ttacctgatg ccgattaagt tctctgaaca ggcttgggaa
      961 gactacctgt tctggcaggc gaaagatcgt tctaccctga agcgaatcaa tacgctattg
     1021 agagatgttc aacgttcgcc ttttgaagga ataggcaagc cagagccttt aaaatataac
     1081 cttgcgggtt tttggtcacg gcgcatcgac gaagagcata ggcttgttta tgcagtacaa
     1141 gacgacacaa ttctggtggc ccagtgccgt taccattatt gatgtttgag tcacagatga
     1201 cgggcttctg caaattaaaa gaggcagctt cgcattattt ccataaccct caagccgcat
     1261 ccccaagcaa tcccgccgct ttcctttatt tccgcgcgcc gacatgtgac aatcgcgtca
     1321 ggaggcatga acattggatc acactgtaga aaatctggtt gaggtcaagg accttcattt
     1381 cgcgtatggc ccgcataagg tgctcaaggg gatcaatctc accataccgc gcggcaagat
     1441 cgtcgcgatc ctgggcagca gcggctgcgg caagaccacg ctgctgcgcc attttggcgg
     1501 gcagttgcgt ccgtcgagcg gcagcgtgaa gttcatgggg caggaggtac acgagctcgg
     1561 taacgacgag ttgtacaaga tgcgtctgaa aatgggcatg atgtttcagg tgagcggctt
     1621 gttcacggat ctgtcggtct atgacaatct ggctttcccg atgcgcgagc acaccgatct
     1681 gtcggaggag atgatccgcg atctggtgtt gatgaagctg aatgcggtgg gcctgcgcaa
     1741 tgcccgcaac ctgaagccta gcgagctttc gggaggcatg gagcggcgcg tggcgctggc
     1801 ccgggcgatc gccaccgacc ctgcattgat catttacgac gaaccctttg ccggtctgga
     1861 tccgatttcg ctcaatacca tcgcgaacct gatccggcga ctgaatgatg cgctcggcgt
     1921 gacttcggtg gtggtgacct acgatctgtc ggagtcgctc aagatcgtcg attacgtgta
     1981 tttcatccat gacggcgtcg tcgtggccga gggggaggcc tcggagatgc tgaattcgca
     2041 agaccccttt gtccgccagt ttgtccacgc cgagccggat ggcccggttg cgttccaata
     2101 ccccagcgag ccgtatgctc aggagctgaa catcagtatt gccgctgcct gacaaacaaa
     2161 aagcgcttct cgatcatgga gaagcgcttc tttgttaaag ggaagtgacg ggcggcgcgt
     2221 agcgggattc tgccgccccc gtcagtgtta atgctgcttt tggtaatgcg aaatcagccc
     2281 gctggtggag gcatcatgcg gtgatgccga gccctctccg cgcagttccg gcagaatttt
     2341 cccggccagt tgcttgccca gttccacgcc ccattgatcg aaggggttga tgttccagac
     2401 cacgctttgc acgaagacct tgtgttcata cagggcgatc agcttgccca gcgtcttggg
     2461 atcgagccgg tcgaacagca gggtattggt cggtttgttg ccggggaaga ccttgtgcgg
     2521 cagcagcgct tccagtgcct cgccttgcag gccctgtgcc tccagttcgg ctcgcgcctc
     2581 catttcgttc ttgccgacca tcagcgcttc ggtctgggcg aagcagttgg cgagcaggat
     2641 ggcgtggtgt tcgccgatgg ggttctggct gcgcatcggt gccagaaagt ccgccgggat
     2701 catccgcgta ccctgatgga tgagctgata gaaggcgtgc tggccgttgg tgccgggttc
     2761 gccccagacc accatgccgg tgtcgtaatc caccggattg ccgtcgcgat cgacgcgctt
     2821 gccgttgctc tccatgtcca gctgttgcag ataggcgggg aagcggtgca gatactggtc
     2881 gtaggggaac acggcattcg cgcccgcgcc gaagaaattg ccgtaccaga tacccagcat
     2941 gcccatcagc accgggatgt tctggtccgg cggcgcatga cgaaaatgtt cgtccatcgc
     3001 ataggcgcca tccagcagcg cttcgaactt gtccatgccg atgaacaagg cgatgggcag
     3061 gccgatggca gaccacagcg agtagcgtcc gccgacccag tcccagaatt cgaacacgtt
     3121 gtgcgggttg atgccgaact gggcggtggc atccagattg gtagagacgg cggcgaagtg
     3181 ttctgccacc gccgtttcgt cgcccagctt gtcgaccagc caggcacgcg ccgaacgcgc
     3241 attggtcatg gtctcctggg tggtgaaggt cttggagctg accacaaaga gtgtcgtttc
     3301 ggcatccagc tttttcagcg tttcggccag atgggtgccg tccacgttgg agacgaaatg
     3361 cgcgcgcaga ttcgctctgc cgtagggctt gagcgcctcg cataccatca gcgggccgag
     3421 atcggagccg ccgatgccga tgttgacgat gtcggtgatc tgcttgccgc tatggccgcg
     3481 atgttcgccg ttgcgcaccg cctcggaaaa gcgccgcatc agtcccagca cgcggcgcac
     3541 gtccggcatc acatcccggc catcgaccag caccggacgg tcggagcggt tgcgcagcgc
     3601 ggtgtgcaat gcggcacggt gttcggtggc attgatcttg gcgccgccga acatctgttc
     3661 gacatgggcg ggcagggcgg cctgttcggc gagtgccagc agcaggctca tggtctccgc
     3721 cgtgatgcgg ttcttggaat agtcgaacag caggtcatcg agtcgaacgg agaaccgttc
     3781 gaaacgcgcc ggatcatcgc ggaacatctg gcgcatggat agcggcccga taacttgatg
     3841 gtgtgccttg agtccttgcc aggcgggcga ttgagtgagt ttcgacataa tggtcaattc
     3901 aaatcaataa cagttaagct tccggtacga gtatgacccc cgccagtggc gggagcgaaa
     3961 tgacggcgga gcagggctgt cccatccacg caatcggctc ggcatggatc aggcccgcat
     4021 tccccatgtt gctgcccgca taaaactgcg aatcgctgtt cagtacttca cggtagctgg
     4081 cctgcttggg caggccgatg cggtaatgct cgcgcggtat gggagtcagg ttcagggcga
     4141 tcacggcact cgatccatcg cgcgcatggc gcagatagga caacaccgac ttctcggcat
     4201 cgttgcagtc tagccagctg aagccatcct gctggaaatc gatatcgtgc aaagcgggca
     4261 actggcgata cagctcgttg agatcatggg tcagattctg cacgccgcga tgaaattcct
     4321 gtccgagctg ccaccattcc agctcccact tggattgcca ctcgcgtccc tgggcgatct
     4381 cgttgcccat gaaattgagt tttttgcccg gccaggtcat ctgaaaggcc atcagcaaac
     4441 gcaggttggc gaacttctgc caggcgtcgc cgggcatctt ggacagcaac gagcctttgc
     4501 cgtgcaccac ttcgtcgtgc gagaagggca gcacgaagtt ctcgctgtag gcgtagatct
     4561 gtccgaaggt gagctggtcg tggtggtagc ggcgatgcac cggatcatgg tggaaatagc
     4621 tcagcgtgtc gttcatccag cccatgttcc acttgatcga gaagcccagt ccgccgaggt
     4681 aagtgggacg ggacacgccc ggccaggcgg tggattcttc cgccatggtc agtgcgccgg
     4741 ggaattcctc atgcaccatg atgttcattt cgcgcaggaa atccaccgca tcgaggttct
     4801 cgcgtccgcc gtaacggttg gggatccatt cgcccgcctt gcgcgaatag tccagataca
     4861 gcatggaggc gaccgcatcc acgcgcagac cgtcgaagtg gaactcggac agccagtaat
     4921 gggcgctgga catcaggaag ctcttcacct cgttgcgccc gtaattgaag atgtgggtac
     4981 cccagtcctg atggaaaccg aggcgcggat cttcgtgctc gaacaacgcc gtgccgtcga
     5041 agcgtgccag cgcccagtta tcctgaggga agtgcgccgg cacccagtcg aggatcacgc
     5101 cgatgtcggc ctgatgacag gtgtcgatca gatggcgcaa atcgtctggt gaaccgaatc
     5161 ggctggtcgg cgcgaaataa ccggtggcct gataacccca agactcgtcg agcggatgct
     5221 cggaaaccgg catgagttcg atatgggtat agcccatgct ttggacgtaa ggcaccagat
     5281 cggtcgccag ttcgcggtaa ctatagaagc ggccatccgg atgacgcttc caagagccgg
     5341 catgcacctc gtagatattg atcgcaccgt gcagccagtc ccattcgccg cgctgtttca
     5401 gccaggcggc atcctgccag cggtgctgat tgggcggggc ggcgagggct gcggtgccgg
     5461 ggcgcagttc atagccctgc gcgtagggat cggttttggt caggatattg ccggtggcgc
     5521 gattgcgaat ttcgtagcgg tacagcgcat gcgacgagat ttccgggagg aacagttccc
     5581 agacaccgtt ggaaccgtgg gagcgcatcg gatgcacgcg accatcccag ccattgaact
     5641 cgcctaccac gctgacgcgc tcggcattgg gtgcccagac tgcgaagcgc acgcctttta
     5701 cgccatcgat ctcgacgctg tgcgagccaa gcatctggta gctttgatgc aatcgtccct
     5761 cgctgaacaa atacaggtcg gagggcgaca ggtgcggcgg gaactggtag ggatcgaagg
     5821 tttcgtgagt cgcgctgccg tagtgcatgc gcagacggta ggggcgggag ggttcggttt
     5881 tgtcgcgcca ctcgaacact ccgcccgggt gtatggcttt gagagccgta ttgcaggttc
     5941 cattcaacag ttcgacgccg gtcgcatgtg gctggtagac ccgaatgacc cattcgtcgc
     6001 cctcacgatg aagacccaac agcccgaaag gatcgtaatg gcgcgcttgc aacagtaaat
     6061 cgaatggcgt attcacatca aagcccccgc tgttctagtt gtgtcgtgag ccgattgtaa
     6121 acgataatgg aagcggcaag caggccaaat agacggagac gcaacatgaa tgctgaactg
     6181 ggttatctgc aaaagaagta ccacctcgcg gctggcgata cctcgccacg tttcatcagc
     6241 agaatcacta aaaataccta tgccatggtg ttggcgggcg gtcgcggcag ccgtctgcat
     6301 caactgaccg attggcgtgc caagcctgcc gttcccttcg gcggaaaatt tcgtatcgtc
     6361 gatttcgtgc tgtcgaattg cgttaactca ggcatccgac gcatcggtgt ggccacacaa
     6421 tacaaatccc atagcctgat ccgtcatatt cagcgcggct ggtcattcct gaatggtcaa
     6481 tttggcgaat tcctcgacat cctgcctgcg caacagcgtg tggatgagga gcattggtat
     6541 caggggacgg cggatgcggt ctaccagaat ctcgacatct tgcgcgagca caatccggag
     6601 ttcgtgctga ttctggcagg cgatcatgtc tacaagatgg actatggcaa gctgctggcc
     6661 tttcatgtgg ccaccaaggc cgacatgagc gtagcttgcc ttgaggtgcc gatcgccgat
     6721 gcaagcgcct tcggcgtcat gggggtggat gaacatggtc gcgtggtgca gtttgacgag
     6781 aaaccggcga acccggcaac cattccgggc aaaccggaca g
//
LOCUS       DASUIV010000052         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_663628, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000052 DASUIV010000000
VERSION     DASUIV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663628"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VFW59_01720"
     CDS             complement(<1..1105)
                     /locus_tag="VFW59_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168387.1"
                     /note="catalyze the formation of cyanophycin which may act
                     to store excess nitrogen; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5362962.1"
                     /translation="MEVSRIRALRGPNLWSRHTAIEAIVSCTGADCSIGDMPGFEDRL
                     RASFPEIGYLQPAGHEEGISMAHVLEVAALSLQKQAGCLVTFSCTVQTVESGVYQVVI
                     EYGEEAVGRLAFEIAQALCSSAVEDTPFDLDQALTRLRELDEEERLGPSTGSIVQAAL
                     ACGIPYRRLTSGSMVQFGWGSQQRRIQAAETDGTSAIAQSIAQDKELTKKLLDAAGVP
                     IPFGRPVLDEDDAWAAAEEIGLPVVVKPQDGNQGKGVTVNIVSRDHLKIAYAAASAIS
                     ELIMVERYLPGHDFRLLVVGGKVVAAARRDPPQVIGDGVHNVRELVEIVNSDPRRSEG
                     HATALTKIRLDEIALARLSIQGLSADSIPPKGSR"
     gene            complement(1184..>3089)
                     /gene="cphA"
                     /locus_tag="VFW59_01725"
     CDS             complement(1184..>3089)
                     /gene="cphA"
                     /locus_tag="VFW59_01725"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5362963.1"
                     /translation="VTIELQNLAGMQTGFGKARETSTRGVYKVVVRSRHATVSRACLE
                     AARDLVMAAIEDRPFDVPATIVRLRDIADSYCLGPSTACIVDAASERKIPSIRLNEGN
                     LVQLGYGARQHRIWTAETDQTSAIGESISSDKDLTKTLLQACGVPVPEGRLVDSPEDA
                     WEAAEEIGVPVVVKPRDGNHGRGVTTELMTREDVASAYKLALGEGSGVLVERFVRGNE
                     HRLLVVGGRVVAAARGESASVLGDGRSTVTELINDQINTDPRRGTTDADPLNVILLDD
                     DAAVRFEILRQGFKPDSIPAKGKQVLIQRNGNVAFDVTDLVHPTIAAAAELAARVVGL
                     DIAGVDLVVEDVSRPFVEQRGAIVEVNAGPGLLMHLKPADGEARPVGQAIVNSLYAAD
                     ESGRIPIVGITGTHGKTTVARLVARLLHLSGKYVGLTCADGLYLDQRQVEKGDRVSWA
                     SAHKILLNRSVDAAVFVNSARSILSEGVAYDRCQVGVVTNIIGAEGLDKFYVHDPEQV
                     YNVMRTQVDIVLPDGVAVLNAHDPLVLKMAELCDGSVILFGLDPEIPAVTAHRDQGGR
                     AVFIRYGRLVMATGRDEVTLVEVASIPLLKSGTPPFQTENVLAAVGAAWALGLSQELI
                     RAGIETFKVD"
BASE COUNT          647 a          981 c          823 g          638 t
ORIGIN      
        1 cgcggctgcc tttaggcgga atcgaatctg ccgatagccc ctgaatcgac aaacgtgcga
       61 gagcgatctc gtccaggcga attttggtca gcgccgtcgc atgtccctca ctgcgccgcg
      121 gatcactgtt tacaatttca accagttcgc gcacgttgtg aactccatcg ccaatcactt
      181 gcggcgggtc gcggcgtgcg gcggcaacga ccttgccacc gaccaccagc aggcggaaat
      241 cgtgcccggg caaatagcgc tccaccataa tcaactcgct gatcgccgag gccgccgcgt
      301 aggcaatctt gagatgatcg cggctgacaa tattgacggt cactccttta ccctggttgc
      361 cgtcctgcgg cttaaccacc accggcaggc cgatctcttc tgctgcagcc caggcatcat
      421 cttcatccag cacggggcgt ccgaacggaa taggaacgcc ggcagcatcg agcaattttt
      481 tggtcagctc tttatcctgg gcgatggact gcgcaatcgc gctggtgcca tctgtttcgg
      541 cagcctgaat gcggcgctgc tggctgcccc agccaaactg caccatgctg ccggaagtca
      601 gacgccgata ggggatgccg caggcaagcg ctgcctgcac aatggagccc gtgctgggtc
      661 ccagacgttc ctcttcatcc agctcgcgca aacgggtaag cgcctggtcg agatcaaacg
      721 gcgtatcttc tactgccgag ctgcaaagcg cttgcgcgat ttcaaacgcc agccggccca
      781 ccgcttcttc accatattcg attaccactt gatacacgcc ggactcaacc gtctgcacgg
      841 tgcagctgaa cgtaaccaga cagcccgcct gtttctgcag gctgagtgca gcgacttcca
      901 gcacatgggc cattgaaatg ccctcttcat gaccggccgg ttgcaagtag ccaatttccg
      961 gaaagctcgc gcgcaggcga tcttcaaaac cgggcatgtc gccgatggaa caatctgctc
     1021 cggtgcagga cacgatggct tcaattgcag tatgtctgct ccagagattt gggccgcgta
     1081 gcgctcgaat acgtgatact tccatcagca atttttcctg tattgcacgg ctggtatttc
     1141 cgccgtgagt tagctaaata atcagattaa ttggaaaatc ggcctagtcc actttaaatg
     1201 tttcgatgcc ggcccgtatc agttcctgcg acagcccgag tgcccaggcc gcgccaacag
     1261 cagccagcac attctcggtc tggaagggcg gcgtgccgct cttcagcagt ggaatggatg
     1321 ccacttcgac cagcgtcacc tcatccctgc ctgtggccat gaccaggcgg ccatagcgga
     1381 taaacacggc gcgcccaccc tgatcgcggt gtgcagtaac agcaggaatt tccggatcca
     1441 gcccaaacaa gatcacgctg ccgtcacaca actcggccat ttttaacacc agcgggtcat
     1501 gcgcattgag cacagccacg ccatcgggca gcacaatatc aacctgcgtg cgcatcacgt
     1561 tgtaaacttg ctcgggatca tgaacataga atttatcgag gccttcggca ccgatgatat
     1621 tggtcaccac gccgacctgg cagcgatcgt aggctacccc ctcagaaaga atggagcgcg
     1681 cgctgttgac aaataccgcc gcatcgaccg agcggttgag taaaattttg tgtgcggatg
     1741 cccaggaaac acggtcgcct ttttcaacct ggcgctgatc gagataaagc ccgtcggcac
     1801 aggtcaggcc cacatatttt ccgctgagat gaagcagtcg cgccaccagc cgcgcaaccg
     1861 tcgtttttcc gtgcgttccg gtgatgccaa caatgggaat gcggccactc tcgtctgcag
     1921 catagagact gttaacgatc gcctgtccga cagggcgcgc ttcgccatct gcgggcttca
     1981 gatgcatcag caggccgggg cccgcattca cttcgacgat tgcaccgcgc tgctcaacga
     2041 atgggcgcga cacatcctcg acgaccagat ccactcccgc aatatccagt ccgacaactc
     2101 gcgctgccag ttctgcggct gcggcaatag tgggatgaac caggtcggtc acgtcgaatg
     2161 cgacattgcc attgcgctga atcagcactt gcttgccctt ggctggtatc gagtccggtt
     2221 taaaaccctg gcgcaggatt tcgaatctga ccgcagcatc gtcatccaga agaatcacat
     2281 tcagcggatc ggcatcggtc gtgccgcgtc ttgggtcagt attaatctgg tcatttatca
     2341 gctctgtaac agtcgagcgt ccatctccca gcacgcttgc cgactcgcca cgggctgcgg
     2401 ccaccacgcg tcctcccacc accagcaggc ggtgttcatt gccgcgcacg aagcgctcaa
     2461 cgagcacgcc actgccctca cccagggcca gcttataagc ggatgctaca tcctcgcgtg
     2521 tcatcaattc tgtcgttaca ccgcgaccgt ggttgccatc acgcggcttc acgacgaccg
     2581 gaacgccgat ctcttcagcg gcctcccacg catcttcagg gctatcaacc agacgaccct
     2641 ccggaaccgg cacaccgcag gcctgcagca gggttttggt gaggtccttg tcgctggata
     2701 tgctctcgcc aatcgcgctg gtctggtcag tctcggccgt ccagatgcgg tgttggcgtg
     2761 cgccataacc caactgcacc agattgcctt cattcagacg gatagacgga atttttcgct
     2821 cggatgcagc atcaacaatg catgcggtac tcgggcccag acagtaagag tcggcgatat
     2881 ctcgcaggcg aacaatcgtc gccggcacat caaaaggccg atcctcgatc gccgccatca
     2941 ccagatcacg ggcagcctcc aggcatgcgc ggctcactgt tgcgtgacgt gagcgcacca
     3001 caactttgta taccccgcgt gtggatgtct cgcgtgcctt gccaaagccg gtctgcattc
     3061 cagccaggtt ttgcagctcg atcgtgaca
//
LOCUS       DASUIV010000053         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_12889, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000053 DASUIV010000000
VERSION     DASUIV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12889"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..652
                     /locus_tag="VFW59_01730"
     CDS             209..652
                     /locus_tag="VFW59_01730"
                     /inference="COORDINATES: protein motif:HMM:NF020480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity protein Tdi1 domain-containing
                     protein"
                     /protein_id="HEX5362964.1"
                     /translation="MSIGWASLTCTPSEESVQQLAVSWGWLIREPFVPVLFTALGDVF
                     FQPDSGGIWWLNTGTAEVTRVADSVDQFRELLGTEIVDEWFLPDLIEQLHAAGKVPDD
                     GECYTYVTLPIFAEGKYEVDNLNPVPAYEHFMLTGDIHCEVLSRP"
     gene            788..1276
                     /locus_tag="VFW59_01735"
     CDS             788..1276
                     /locus_tag="VFW59_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362965.1"
                     /translation="MIETATLITLCMLFLIFFRPGKTPPLENPLVIERAGKYQMTLAP
                     QLNLAQGFVEEIAKRVAMSPASSAPQYFAVHDKQVTAHGFDFYLLAVTQRNGMLYFQA
                     ARPSSKDIAEQLPVISGFADQTLTRFPDEGEHDALLDRALCDAVREVAHMRGIGIGQL
                     TV"
     gene            1325..1909
                     /locus_tag="VFW59_01740"
     CDS             1325..1909
                     /locus_tag="VFW59_01740"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEX5362966.1"
                     /translation="MQLDDSGLDLIFRAARTHSVWQDKPVGDALLRQVYDLMKWAPTS
                     ANCSPARIVFVRSAQAKQRLLPAIAPGNEEKTRTAPVTAIVAQDYEFYEKLPTLFPHA
                     DARSWFAGNQPVIDTTAFRNGTLQGAYLILAARALGLDAGPMSGFDNEKVDREFFAGT
                     KIKSNFLINLGYGDASKLYPRSPRLDFDEAAQIL"
     gene            complement(1913..>3176)
                     /locus_tag="VFW59_01745"
     CDS             complement(1913..>3176)
                     /locus_tag="VFW59_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5362967.1"
                     /translation="CVGMLVRAHLVTTDLGLLAGCAMMLYGFSLSQEYAVRAGFWIGT
                     GVGIGFMTKGFIAPVLFVFIAAALPLLFDAWRVRRYFSSLAVAALSALPWLTVWPLLL
                     FQHAPHLFVEWAWTQNISHWLQYAQHGPTLESLYFLENLPWLAWPALPLAIWVVWQSR
                     RRLAMRDDLQLPLVAFGVMLVTLSLIPNIQEVFALPMLLPLTLLATASLSALRRGAAN
                     ALDWFGLMTFALLAIAMWWGWAGLLLDNDAKITHWLKDYQPGFEPEFHAVPFWIAVAA
                     SVLWLVLVWRVGRSMRRAVTNWAAGITLIWVLAMTLWLPWLDSGKSYRSMVVSLKHAM
                     PKHYRCVGYDYLGDSQRAMLHYFGDIVTHEDRKERCDLRIIQGNRLSKPLLDETRWKK
                     IWEGSRKGDKYEHFRLYQRIATERNAAK"
BASE COUNT          633 a          915 c         1004 g          624 t
ORIGIN      
        1 gcacccattc atcaggggtt gtgggcggta cacaagacgg gcgtgctgcc caggttgctg
       61 aatctggcgg cgggtcgcga acccttggaa cgcatcagca ttgttcaggt tgggatgccg
      121 tcagaatgag ctcgccgtca ggtgcatgtg ccctttgtgc tcagcgcagg attttcaccc
      181 cgtaaaactc aattaaggta ggtcactcat gtccataggc tgggcttcac tcacttgcac
      241 accatccgaa gagtcggttc aacagctcgc ggtgtcctgg ggctggttga tccgcgaacc
      301 tttcgtgcct gtgctcttta ccgcgctcgg cgatgtgttc tttcagcccg actcgggcgg
      361 catctggtgg ctcaacaccg gtacggcaga ggtgacgcga gtcgccgatt cggtcgatca
      421 attccgcgag ttgctgggta cggaaatcgt cgacgagtgg tttttgcctg atctgatcga
      481 acaattgcat gcggcaggaa aagtgcccga cgacggcgag tgctatacct atgtgacgct
      541 tcccatcttt gcggagggca agtatgaagt cgacaatctc aatcccgttc cggcctatga
      601 gcacttcatg cttacggggg atattcattg cgaagtcctc tcgcgtccct aaccagcaac
      661 ttgccatcgt tgttcgatgg cttagttgaa tggctagttg ttcttccatt ggcaggtaaa
      721 gagtgaatcc gatgttgtgt agttaagccg gttcattgac ggcaatgttt aaacgggaaa
      781 gacactcatg atagaaaccg cgacgctgat tacgctctgc atgctgttcc tgatcttctt
      841 ccggcccggc aagacgccgc cgctggagaa tccgctggta atcgaacgcg cggggaagta
      901 tcagatgacc ttggcccctc agctcaatct ggcacagggt tttgtcgagg agatcgcgaa
      961 gcgggtcgca atgagtcctg ccagcagtgc gccgcaatat tttgcggtgc atgataagca
     1021 ggtcaccgcg catggtttcg atttctatct gctggccgtg acccagcgca acggcatgct
     1081 gtactttcag gcggccaggc catcttccaa ggatattgcg gagcagctgc ccgtcatcag
     1141 cggatttgcc gaccagacgt tgacgcgttt cccggacgag ggggagcatg acgccttgct
     1201 ggatcgggcc ttgtgtgatg cggtgcggga agtggcgcat atgcgcggca tcgggatcgg
     1261 gcagttgacg gtatagtgtg acgagcgaca ttcatcgaac gcagtgctca acaaggagat
     1321 aaccatgcaa ctcgacgaca gcggtctgga tctgattttt cgcgcggctc gcacgcatag
     1381 cgtgtggcag gacaagccgg tcggcgatgc gctattgcgg caggtctacg atctgatgaa
     1441 gtgggcgccg accagcgcca attgcagtcc ggcgcgcatc gtgttcgtgc gcagcgcaca
     1501 ggcgaagcag cgtctgttgc ctgcgatcgc gcccggcaac gaagagaaga cccgcaccgc
     1561 acccgtgacg gcgatcgtcg cgcaggacta cgagttctac gagaagctgc cgacactgtt
     1621 tccccatgcc gatgcgcgtt cctggttcgc aggcaaccag ccggtgatcg ataccacggc
     1681 gtttcgcaac ggcacgctgc aaggcgctta tctgatactg gccgcgcgcg cactcggact
     1741 cgatgccggg ccgatgtcgg gattcgataa cgagaaggtg gatcgggagt ttttcgcggg
     1801 cacgaagatc aaatcgaact tcctgatcaa tctcggttac ggcgatgcca gcaagctgta
     1861 cccgcgcagt ccgcgcctcg atttcgacga ggccgcgcag attctgtaac gcttatttgg
     1921 ctgcgttccg ctccgtggcg atgcgctgat acaggcggaa gtgttcgtac ttgtcgccct
     1981 tgcggctgcc ttcccagatc tttttccagc gggtttcatc cagcaggggt ttggatagcc
     2041 tgttgccctg aatgatgcgc agatcgcagc gctccttgcg atcctcgtgg gtgacgatgt
     2101 cgccgaagta atgcagcatc gcacgctggc tgtcgcccag gtagtcatag ccgacgcagc
     2161 ggtagtgctt gggcatggcg tgtttcagcg agacgaccat gctgcggtag ctcttgccgc
     2221 tgtccagcca gggtagccac agcgtcatgg cgagcaccca gatgagggtg atccccgcag
     2281 cccagttggt gacggcgcgg cgcatcgagc ggccgacgcg ccagaccagc accagccaca
     2341 gcacgctcgc cgcaacggcg atccagaatg gtacggcgtg gaactccggc tcgaagcccg
     2401 gctggtagtc cttcagccag tgcgtgatct tggcatcgtt gtccagcaac aggccggccc
     2461 agccccacca catcgcgata gcgagcagcg caaaggtcat caggccgaac cagtccagcg
     2521 cgttggcagc gccccgtctc aatgccgaca gcgaggcggt ggcgagcaag gtgaggggga
     2581 gcagcatcgg cagcgcgaac acctcctgaa tgttgggtat caggctgagg gtgaccagca
     2641 tcacgccgaa ggcgaccagc ggcagttgca gatcgtcgcg catcgccaga cggcggcgcg
     2701 attgccacac gacccagatc gctagcggca gcgcaggcca tgccagccat ggcaggttct
     2761 ccagaaagta gagcgattcc agcgtggggc cgtgttgcgc gtactgcagc cagtgactga
     2821 tgttctgtgt ccacgcccac tcgacgaaca ggtggggggc gtgctggaac agcagcagtg
     2881 gccagaccgt gagccagggc agcgcggaga gcgcggccac ggcgaggctg gagaaatatc
     2941 ggcgtacgcg ccaggcgtcg aacagcagag gcagggccgc agcgatgaag acgaacagca
     3001 cgggagcgat gaagcccttg gtcatgaaac cgatgcccac tccggtgccg atccagaaac
     3061 ccgcgcgtac cgcatattcc tggctcagcg agaagccgta tagcatcatc gcgcagcccg
     3121 ccagcaggcc gaggtcggtg gtgaccagat gggcgcgcac cagcatgccg acgcag
//
LOCUS       DASUIV010000054        16918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_848739, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000054 DASUIV010000000
VERSION     DASUIV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 16918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16918
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848739"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFW59_01750"
     CDS             complement(<1..596)
                     /locus_tag="VFW59_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX5362968.1"
                     /translation="MNTTVDAVMNPDSFAAMFEESLTRKEMRAGELITAQVVRIDHNM
                     VVVNAGLKSESLIPVEEFLSDKGEMEVAAGDFVTVAIESLENGYGETKLSREKAKRLA
                     AWHDLELAMEEGKVVEGFVSGKVKGGMTAMVNGIRAFLPGSLVDIRPVKDTTPYENKL
                     MELKVIKLDRKRNNVVVSRRAVLEETMGADREAIMENL"
     gene            complement(671..2623)
                     /locus_tag="VFW59_01755"
     CDS             complement(671..2623)
                     /locus_tag="VFW59_01755"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF008816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HEX5362969.1"
                     /translation="MTSENFLDLPPLMSARGTVTLPGSKSISNRVLLLAALSEGVTEV
                     RDVLLSDDTERMLEGLQKLGVGVEKLDTHVFRVTGCGGNFPIKEAELFLGNAGTAFRP
                     LTAALALSGGHYKLSGVARMHERPIGDLVDALRQLGADIRYLGNDGFPPLEIFPAKLA
                     GDTVQVRGDVSSQFLTGLLMALPLTGQTVKVEVVGELISKPYIEITLAMMARFGVKVE
                     RKDWQSFVVFGGQRYKSPGVIFVEGDASSASYFLAAGAIGGGPVRIEGVGSDSIQGDV
                     RFADALALMGARIAQGPNWMEAQAPASGRLRAIDLDCNHIPDAAMTLAVAALFADGTT
                     TLRNIASWRVKETDRIAAMAAELRKVGATVEEGADFIRVTPPKSQILNPESGIDTYDD
                     HRMAMCFSLVAFGGAGVRINDPGCVAKTFPEYFARFNEVVQAVPVIAIDGPSASGKGT
                     VAGRVATTLGFHYLDSGALYRLLALAAFRDGIALDDEAALTRLAGKIEIRFEGSDVWL
                     DGVPVGDELRSEETGGYASQVAALPQVRAALLDKQRSFRRAPGLVTDGRDMASVVFPD
                     AATKIFLTASAEARADRRYKQLKEKGMGANIAALLQDIRARDERDTQRSAAPLQQAPG
                     ASLLDTTSLNIEQAVQEVLNRYQANN"
     gene            complement(2801..3181)
                     /gene="acpS"
                     /locus_tag="VFW59_01760"
     CDS             complement(2801..3181)
                     /gene="acpS"
                     /locus_tag="VFW59_01760"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX5362970.1"
                     /translation="MIHGIGTDIVEFSRIEQIWARHGQRFAERILSARELPELDGHAA
                     PARLLAKRFAAKEAFAKAVGSGLRHPVSLQRISITHDGLGRPVLCFDEMLRIHLAQLG
                     INGHHLSISDERGMIVAFVVLESV"
     gene            complement(3206..3925)
                     /gene="pdxJ"
                     /locus_tag="VFW59_01765"
     CDS             complement(3206..3925)
                     /gene="pdxJ"
                     /locus_tag="VFW59_01765"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX5362971.1"
                     /translation="MKLGLNIDHVATLRQARGARYPNVVRAALICEEAGADAITLHLR
                     EDRRHIQDRDVEVLRDMLQTRMNLECAVTDEMIKLALRTRPHDICLVPERREELTTEG
                     GLDVVRYFDAVKSATERCLDAGIRVSLFIDPDMKQIDAARSIGAPVIELHTGSYAEAD
                     SVPERAQELERIRAATAHAHAQGLQVNAGHGLHYHNVQDIVAIPNIVELNIGHAIIAE
                     SLFIGLDAAVRKMKALLAEHS"
     gene            complement(3922..4650)
                     /gene="recO"
                     /locus_tag="VFW59_01770"
     CDS             complement(3922..4650)
                     /gene="recO"
                     /locus_tag="VFW59_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5362972.1"
                     /translation="MTAANRNKQQDEPAFVLHSYPFRETSLILDVFSRQHGRLAIMAR
                     GARRPRSALRGLLMNFQPLLLSWFGKGEVRTLHSAEWQGGQPYLQGTALMCGFYLNEL
                     LINLLARDDPHEQLFDYYRATLYRLAHESDHAATLRCFEKHMLQELGYALPLTHEVRS
                     GEPICAEVCYRYLVERGPVPDEGESEAGLTIAGKTLLDMAADEYIDATTVQQSKQLMR
                     MLLNHYLGGKTLYTRELIKDLQKL"
     gene            complement(4647..5573)
                     /gene="era"
                     /locus_tag="VFW59_01775"
     CDS             complement(4647..5573)
                     /gene="era"
                     /locus_tag="VFW59_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5362973.1"
                     /translation="MTEQDPSSGDAESTAETFRSGFVAIVGRPNVGKSTLLNHLIGQK
                     VSITSRKAQTTRHRIHGIRTDKNAQYVFVDTPGFQTKHLNALNRGMNRVVTSSLRDVQ
                     VVVFVLEALRYDDRDREVLRLLPDNRPVLLVINKIDEVPDKGQLFSFAERVAADFKFA
                     AIVPVSAKQGTKVEELVEALYRYMPEGEPIYDENDITDRSERFLAAEMLREKVFRFTG
                     EELPYSVSVVIEQFKMEGKLRRISAAILVDKEVHKAMLIGKKGEKLKEIATQARLDME
                     KLFDGKVFLEVFIKVRSGWADDARVLRQLGYE"
     gene            complement(5570..6253)
                     /gene="rnc"
                     /locus_tag="VFW59_01780"
     CDS             complement(5570..6253)
                     /gene="rnc"
                     /locus_tag="VFW59_01780"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5362974.1"
                     /translation="MASRDRRTLSKQLGHVFAQPQLLERALTHRSSAPEHNERLEFLG
                     DSVLGCVIAKHLYLSYPQLSEGDLTRLRSNLIKEETLAGLAQQLELGSYLQLGEGERK
                     SGGFRRPSMLADAMEALFGAVLLDGGFEAAEKVVLSLFVPYLAQVDVQTLGKDAKTLL
                     QEYLQARRIALPAYSIVEVQGDAHAQSFRVECAIPALKITTHGEGSSRRNAEQQAALS
                     AYQQLGKKS"
     gene            complement(6243..6614)
                     /locus_tag="VFW59_01785"
     CDS             complement(6243..6614)
                     /locus_tag="VFW59_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEX5362975.1"
                     /translation="MNTAMPAQQRGLTFFGFIFGAFLVVLLSITGLKLIPAYMQNETI
                     KNLFVTVAHDPEMQGANVGSIKMSYIRRASVEDIRAITPDDIEISKDNGVLKLSASYA
                     VKIPLVANISLYLEFNPSSGK"
     gene            complement(6627..7430)
                     /gene="lepB"
                     /locus_tag="VFW59_01790"
     CDS             complement(6627..7430)
                     /gene="lepB"
                     /locus_tag="VFW59_01790"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5362976.1"
                     /translation="MDFALILFLLLLVTGSVWLLDRFALAGRRADGVAEPWWVEYAKS
                     FFPVILVVFFIRSFLVEPFKIPSGSMIPTLQVGDFILVNKFNYGIRLPIINQKIVQIN
                     DPQRGDVMVFHYPENPSLDYIKRVVGLPGDTVEYRDKRLWVNGVEQIQKSDGDYNYVE
                     SGLSFVHTEKRIETLGEHKHAVLVNPEMPTLHLSSVAEFKGREGCSYDDEMVRCKVPE
                     GQYFMLGDNRDNSRDSRYWGFVPDHQIVGKAFFIWMNFSDLKRIGLSIN"
     gene            complement(7439..9232)
                     /gene="lepA"
                     /locus_tag="VFW59_01795"
     CDS             complement(7439..9232)
                     /gene="lepA"
                     /locus_tag="VFW59_01795"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX5362977.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRIIHLCGGLSDREMEAQVLDSM
                     DIERERGITIKAQTAALSYKARDGQVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLGVEVVPVLNKIDLPSADPDNAIAEIEDVIGIDAHDAVH
                     CSAKTGLGVEDVLESLIVKVPAPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLK
                     PKEKILFMATGAQHLTEHIGVFTPKAQDRESLSAGQVGFVIAGIKELKDAKVGDTVTL
                     AGRPASSALPGFKEVQPQVFAGLFPVESNQYEALRDSLEKLKLNDAALMYEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVLRDGTVLMVDNPSKLPD
                     PSKIEEIREPIVTVNLYMPQEYVGSVITLCTQKRGVQIDMNYHGKQVKLIYEMPMGEI
                     VLDFFDRLKSVSRGYASMDYEFKEYRPADVVKVDMLINGDKVDALAVIVHRANSQYRG
                     REVAAKMRELIPRQMYDVAIQATIGSNIIARENVKAMRKNVLAKCYGGDISRKKKLLE
                     KQKAGKKRMKQVGNVEIPQEAFLAILRVEDK"
     gene            complement(9315..10718)
                     /locus_tag="VFW59_01800"
     CDS             complement(9315..10718)
                     /locus_tag="VFW59_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX5362978.1"
                     /translation="MLRKFFVLLAMGLMLSPAVQARELPDFTDLVERQGPAVVNISTT
                     QIIRNAPGFPGLQEGDPFFEFFRRFAPPMPQGQAPREQESQSLGSGFIISADGYIMTN
                     AHVVDHAEKITVRLTDKREYSAKVIGADRRTDVALLKIEAAGLPQVSLGSPDKLKVGE
                     WVVAIGSPFGFDSSVTAGIVSAKGRSLPQDNFVPFIQTDVAINPGNSGGPLFNMNGEV
                     VGINSQIYTRSGGSMGLSFAIPIDVATQVTDQLRTTGKVTRGRIGVTIQELTRELADS
                     FGLSKPNGALISSVEKNGPADKAGIEASDVILKFDGKAVNSSADLPRLVAATKPGSKA
                     VMELWRKGASRSVTVVIAEMPEESRLAQAVKKLEDDVAEMISRLGIAVSELSREQLQE
                     LDVVGGLLVEQVKGQAAHAAGLQRGDLLLAVGNMPIRSLAQFNDILKQVPKGRNVALL
                     VRRGDAASYVAIRLDEK"
     gene            complement(10759..11199)
                     /locus_tag="VFW59_01805"
     CDS             complement(10759..11199)
                     /locus_tag="VFW59_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HEX5362979.1"
                     /translation="MTLETRAIVVRIDGPLAFVESAQASGCGQCKSTGCGAGKLSGLL
                     CREPRQFQVDNAIGAAVGDEVVVSVADGAVLRGIGLVYLLPLSLLVAGAACGNAIATL
                     DEQRDGYAAAGAALGLVLGFIVSRWLDKRQSRQLPRIVRQEREG"
     gene            complement(11196..12089)
                     /locus_tag="VFW59_01810"
     CDS             complement(11196..12089)
                     /locus_tag="VFW59_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX5362980.1"
                     /translation="MDWLKTVAFAGHQTDYSGVFIYQYDNRVETSRITHVVEADGEYE
                     KLECMDGPKREIIRHHGHIWEYSNHKMVYVGQQGRGRFPSLLPEQMSALNLNYQAKQV
                     GVERIAGFDAQVILFQPKDQLRYNHKLWVHTDSGLLLKTVVLGDKNQPVEQYAFTQLQ
                     IGGGIDRSWIKSAPLASATTGGIAGDASPAGNGVPVNSGWVVDALPEGFKKTMEIQRP
                     MRGRHAPVVQLVFSDGLSAISVFIEPNDKDEDDSAGLSSRGAMNLYHKVADGHLFTVV
                     GEVPGRTVMQVIDSLRYNGTR"
     gene            complement(12167..12661)
                     /locus_tag="VFW59_01815"
     CDS             complement(12167..12661)
                     /locus_tag="VFW59_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEX5362981.1"
                     /translation="MKQNISTLMDGELFEDEAGDLFGQVRRDANARKDWEIYHLIGDV
                     LRQPEHIHCDLSAKVRERMQDEPVVLAPRVRASKQKVRAFALSAAASLLAVGVVSWMS
                     LQISPEAAPQLAMQQGGLRPASAQIQPKANDYLMAHQEFSPSADMSGGASYLRTVSYR
                     PEGK"
     gene            complement(12664..13263)
                     /gene="rpoE"
                     /locus_tag="VFW59_01820"
     CDS             complement(12664..13263)
                     /gene="rpoE"
                     /locus_tag="VFW59_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEX5362982.1"
                     /translation="MADREVDQLLVERVQRGDKHAFDLLVSKYQRRLGRLISRFVRDP
                     AEAEDVTQDAFIKAYRALPGFRGESAFYTWLYRIGINTAKNHLLANKRRAPTSTLFDT
                     DDAESFEEASLLHEVSTPENELMSKQVVDVVQASLQQLPEDLRSALTLREIEGLSYEE
                     IASVMNCPIGTVRSRIFRAREAVAENLRPLLETSKGNRW"
     gene            13472..15052
                     /gene="nadB"
                     /locus_tag="VFW59_01825"
     CDS             13472..15052
                     /gene="nadB"
                     /locus_tag="VFW59_01825"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX5362983.1"
                     /translation="MLKFDTLIIGSGLAGLTLALKLADHQRVGLITKKSLQDGASSWA
                     QGGIAAVLSENDSIDAHIQDTLIAGAGLCDEAATRHVVEHSKAAIDWLIAQGVPFTRD
                     DTSNMGYHLTREGGHSQRRIIHAADATGHAVQETLSARVLRHPNITVLENHMSVDLIT
                     GKNLGQTDNRCYGAYALNCLSDEVITVVAQNTILATGGSGKVYLYTTNPDTATGDGIA
                     MAWRAGCRIANMEFIQFHPTCLYHPHAKSFLISEAVRGEGGILKLPDGTRFMPAHDER
                     AELAPRDVVARAIDYEMKKHGLDCVYLDISHQSVEFLQEHFPTIYARCLKLGINISRE
                     PIPVVPAAHYTCGGVMTDLHARTDVEGLYAVGETAYSGLHGANRLASNSLLECLVFAD
                     AAIRDILDKPHLTPPPLPAWDESRVTDADEQIVISHNWAELRHFMWDYVGIVRTNKRL
                     QRAQHRIQLLQDEINEYYTNFHISPDLLELRNLVMNAELIVQSALLRHESRGLHFSKD
                     YPETLPNPQPTILTPPPR"
     gene            complement(15049..15450)
                     /locus_tag="VFW59_01830"
     CDS             complement(15049..15450)
                     /locus_tag="VFW59_01830"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEX5362984.1"
                     /translation="MAIHPSPRFVFLLLFSHSLAAGAAYVTVMPSAFKIVIFPLLLLS
                     VLYHLARDALVLLPDSWCGLSVKQGEVSVLRRDAGVMIGNVVDGTFVSPYFVLLRLRI
                     EGRRWLVSRVIFPDALERDVFRDLCVRLRLG"
     gene            complement(15467..16693)
                     /locus_tag="VFW59_01835"
     CDS             complement(15467..16693)
                     /locus_tag="VFW59_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5362985.1"
                     /translation="MISQLGRYEVLGELGQGAMGVVYKAKDPLIDRIVAIKTINLGLA
                     LDEKEEYEGRFYQEAKAAGRLNHPNIVTIYDVGKSGDVAYIAMEFLQGRELRDIMNDG
                     ERLPVEQVLEIVAQVALGLAYAHEHEIVHRDVKPSNIMVVRDGHVKITDFGIARMASA
                     AVRTQTGMVLGSPKYMSPEQVMGKNIDQRSDIFSLGVMLYEMLTGQAPFSGENVNAIM
                     YQTLNAAPPPPSQVNPSAPEMIDFIVAKALAKSVEDRYQNAKDFAADLRACRDTLPRS
                     GQPIDVSKRPAGGEKKLPDAISVTGGRHVEAEEVGAASTAKLSQAFDSGAATMKLAAM
                     TASSEDVDELSKTLKMARPAIGAINRAAPPPVNKAAKLPPVAAHQDREVSRSGGGLLL
                     AIIVLALLGLIVFFVI"
BASE COUNT         3457 a         5522 c         4594 g         3345 t
ORIGIN      
        1 ttgaggtttt ccatgatcgc ttcacgatca gcgcccatgg tctcttccag tactgcacga
       61 cgggaaacca cgacgttgtt gcgcttgcga tccagcttga tgaccttcag ctccatcagc
      121 ttgttttcgt atggagtagt gtccttgacc ggacggatgt caaccagtga acccggaagg
      181 aacgcgcgga ttccgttaac catcgcagtc ataccgccct tgaccttgcc gctgacgaag
      241 ccttcaacga ccttgccttc ttccatggcc agttccagat cgtgccatgc agccagacgc
      301 ttggccttct cgcgcgacag ttttgtctcg ccatagccgt tctccagcga ttcgatcgca
      361 acggtgacga agtcacctgc agcaacttcc atttcgcctt tgtcgctgag gaattcctca
      421 acagggatca agctttcgga cttcagtcct gcattcacaa ccaccatgtt gtgatcgata
      481 cgcacgactt gtgcggtgat cagttcgcca gcgcgcattt ccttgcgcgt caagctttct
      541 tcaaacatag cggcaaaact atcgggattc attacagcgt ctacagttgt gttcatcgga
      601 tacacctgga aaatactctg tttacacaga gggttagtta atgatcccgg aggcggagag
      661 ccaacggggc ctagttattt gcctgatagc gattcaagac ttcctgcacc gcctgctcga
      721 tgttcagaga ggtcgtatcc agcaagctcg cgcccggcgc ctgttgcagc ggagccgcgc
      781 tgcgttgagt atctcgctcg tcgcgtgccc ggatatcctg caaaagggcg gcgatattag
      841 cacccatccc cttctctttc aactgtttat agcgacggtc ggcgcgcgcc tccgcgctgg
      901 cggtcaaaaa tatcttggtc gccgcgtccg gaaacaccac cgaagccatg tcgcgcccat
      961 cggtcaccag ccccggcgcg cgacggaagg agcgctgctt gtccagcaac gcggcgcgca
     1021 cctgcggcaa agccgccacc tgagaggcgt aaccgcccgt ctcctcggaa cgcaactcat
     1081 cgcccaccgg cacgccgtcc agccacacat cgctgccttc gaagcggatc tcgatcttcc
     1141 ctgccagccg cgtcagcgca gcctcgtcgt ccagcgcgat gccatcgcga aacgctgcca
     1201 gtgccagcaa acggtacagc gcaccgctat ccagataatg gaagccgagt gtcgtggcga
     1261 cgcgtccggc caccgtcccc ttgcccgaag cggaaggccc gtcgatggcg atcaccggca
     1321 cggcctgcac aacctcattg aagcgcgcga aatattccgg gaaggtcttc gcaacacagc
     1381 ccggatcgtt gatgcgcaca cccgcaccgc caaacgccac cagcgagaag cacatcgcca
     1441 tgcggtggtc gtcgtaggtg tcgataccgg attcaggatt gaggatttgg gatttggggg
     1501 gcgtgacacg aataaagtcc gcgccctctt ccaccgtcgc acccaccttg cgcaactcgg
     1561 ccgccatcgc cgcgatgcgg tcggtttcct tgacgcgcca gctcgcgatg ttacgcagcg
     1621 tggtcgtacc gtcggcgaac agcgcggcca ccgccagcgt catcgcggca tcggggatgt
     1681 ggttgcaatc gagatcgata gcccgcaacc tgccgctcgc gggcgcttgc gcctccatcc
     1741 agttcggccc ctgcgcgatg cgcgcgccca tcagcgccag cgcatcggca aagcgcacgt
     1801 caccctgaat gctgtcgctg ccaacgccct cgatgcgcac cggcccgccg cctatcgcgc
     1861 ctgctgcgag aaaatacgag gccgacgaag catcgccctc gacgaagatg acaccgggac
     1921 tcttgtagcg ctgcccgccg aacaccacaa aactctgcca gtctttacgc tctaccttga
     1981 ccccgaagcg agccatcatt gccagggtga tctcgatgta gggtttggag atcagctccc
     2041 cgaccacctc gaccttgacc gtctgaccag tcagcggcaa tgccattaac aaaccagtca
     2101 ggaactggct ggacacatcg ccgcgcacct gcaccgtatc tcccgccagt ttcgccggaa
     2161 agatctccag cggcggaaaa ccgtcgttgc ccagataacg aatgtctgcg ccgagctggc
     2221 gcaaagcatc caccagatcg ccgatcggcc gctcgtgcat gcgcgccacg cccgacagct
     2281 tgtaatgacc accggacaac gccagcgccg ccgtcagcgg acggaacgcc gtgcccgcat
     2341 tgccgagaaa cagctcggcc tctttgattg gaaaattccc gccgcagccg gtcacgcgga
     2401 acacatgggt atccaacttc tccacgccga cacccagctt ctgcaagcct tcaagcatgc
     2461 gctcggtgtc gtccgacagc agcacgtcgc gcacctcggt cacgccttcg gacaacgccg
     2521 ccagcagcag cacgcgattg gaaatgctct tcgagccggg cagcgtcacc gtgccgcgcg
     2581 cgctcatcag aggaggaaga tcgaggaagt tttcggatgt catcggaagt ggaccctgca
     2641 acggactgca ctaaaaattc gagggcggca tgataccgca aagcatggct gcagcctaag
     2701 agggcagcgc cctgccccac ggacaagagg gggcagtcag gtggagagga agcaactcta
     2761 gccacgctca agtactgccg agcccctgcg tcaagacggc ctacacactc tccagcacca
     2821 cgaacgcgac gatcatgccg cgttcgtcgc tgatcgacag gtgatgtccg ttgatgccca
     2881 gctgggcaag atggatgcgc agcatttcgt cgaaacacaa caccggacgt cccagaccat
     2941 cgtgcgtgat gctgatgcgc tgcaagctga ccggatgacg caggccgctg ccaaccgcct
     3001 tggcgaaggc ttccttggcg gcgaagcgtt tggccaacaa acgtgccggt gcggcatgcc
     3061 catccagctc cggcaattcg cgtgcgctca gaatacgttc ggcaaagcgc tgaccatgac
     3121 gcgcccatat ctgttcgatg cgggagaatt cgacgatgtc ggttccgatg ccgtggatca
     3181 tgacaagcct accgccgcgt gcgttttatg aatgttcggc cagcagcgcc ttcatcttgc
     3241 gcactgccgc atccagcccg atgaacagcg actcggcgat gatggcgtgg ccgatgttca
     3301 gctcgacgat gttgggaatg gcaacgatgt cctgcacgtt gtgataatgc aggccgtgac
     3361 cggcattgac ctgcaggccc tgcgcatggg cgtgggccgt cgcagcgcgg atgcgctcca
     3421 attcctgtgc gcgctcaggc acgctgtcgg cctcggcgta gctgccggtg tgcagttcga
     3481 tcaccggtgc gccgatgctg cgcgccgcgt cgatctgctt catgtccggg tcgatgaaaa
     3541 gcgacacgcg gatacccgca tcaaggcaac gctcggtggc cgacttgacc gcatcgaagt
     3601 agcgcaccac gtccagtccg ccttcggtgg tcagctcctc gcgccgttcc ggcaccagac
     3661 agatgtcgtg cggccgggtt cgcagcgcca gcttgatcat ctcgtcggtc accgcgcatt
     3721 ccagattcat gcgcgtttgc agcatgtcgc gcaacacttc cacgtcgcga tcctgaatat
     3781 ggcggcgatc ttcgcgcagg tgcagcgtaa tggcatccgc gcccgcctct tcgcagatca
     3841 gcgcggcgcg caccacgttg ggataccgcg caccccgcgc ttggcgcagc gtggcgacat
     3901 ggtcgatgtt cagtccgagt ttcatagctt ctgcaaatcc ttgatcagtt ctcttgtgta
     3961 cagtgtcttg ccgccgagat aatggttcag cagcatgcgc atcagttgct tgctctgctg
     4021 caccgtggtc gcatcgatgt attcatccgc agccatgtcc agcagggtct tgcccgcgat
     4081 cgtcagaccg gcttccgact cgccttcgtc cggcaccggg ccgcgctcga cgaggtaacg
     4141 atagcagact tccgcgcaga ttggttcgcc gctacgcacc tcgtgcgtca gtggcagcgc
     4201 ataacccagc tcctgcaaca tgtgcttctc gaaacagcgc agcgtggcgg catgatcgct
     4261 ttcgtgcgcc agacggtaga gtgtggcgcg gtagtagtcg aacagttgct cgtgcggatc
     4321 gtcgcgcgcc agcagattga tcagcagctc gttcagatag aagccgcaca tcagcgccgt
     4381 gccctgcaga tagggctggc cgccctgcca ttccgcgctg tgcagcgtgc gcacctcgcc
     4441 cttgccgaac caggaaagca acagcggctg aaagttcatc agcaggccgc gtagcgccga
     4501 tctcggccgt ctggctccgc gcgccatgat tgccagcctg ccgtgctgac ggctgaatac
     4561 gtcgaggatc agactggttt cgcgaaacgg atagctgtgc agcacgaagg ccggttcgtc
     4621 ctgctgctta ttccggttcg ctgcggtcat tcgtaaccca gctgacgcaa cacgcgtgca
     4681 tcgtcggccc agccgctgcg caccttgatg aacacttcga ggaacacctt gccgtcgaac
     4741 agtttttcca tgtccagacg cgcctgcgtg gcgatctctt tcagcttctc gcccttcttg
     4801 ccgatcagca tcgccttgtg cacctccttg tccaccagga tcgcggcact gatgcggcgc
     4861 agcttgcctt ccatcttgaa ttgttcgatc accacgctga ccgaatacgg cagctcttca
     4921 ccggtgaaac ggaacacctt ctcgcgcaac atctctgcgg caagaaaacg ttcgctgcgg
     4981 tcggtgatgt cgttctcgtc gtagatcggc tcgccctcgg gcatataacg gtacaaggcc
     5041 tcgaccagct cctccacctt ggtgccctgc ttggcactga ccggaacgat cgcggcgaac
     5101 ttgaagtccg cagccacgcg ctcggcaaac gagaacagct gccccttgtc cggcacttcg
     5161 tcgatcttgt tgatcaccag cagcaccgga cggttatccg gcagcagcct gagcacctcg
     5221 cggtcgcggt cgtcgtagcg caacgcctcc agcacgaaca ccaccacctg cacatcgcgc
     5281 agactgctgg tgacgacgcg gttcatgccg cggttcagcg cgttcaggtg cttggtctga
     5341 aagcccggcg tatcgacgaa cacatattgc gcattcttgt cggtacggat gccatggatg
     5401 cgatggcgcg tggtctgcgc cttgcgcgag gtgatgctga ccttctgccc gatcaaatga
     5461 ttgagcagcg tggacttgcc cacattgggc cgtccgacga tggcgacgaa gccgctgcgg
     5521 aatgtttcag ctgtgctctc ggcgtcgccg cttgatggat cttgttcggt catgattttt
     5581 ttcccagttg ttggtaggcg gacagtgccg cctgttgttc ggcgttgcgg cgactgctgc
     5641 cctcgccatg cgtggttatt ttcagcgccg gaatggcgca ctccacccgg aaggattggg
     5701 catgcgcatc gccctgcacc tcgacgatac tgtaagcagg cagcgcaatg cggcgagcct
     5761 gcagatattc ctgcagcagg gtcttggcat ccttgcccag cgtttgcaca tccacctgtg
     5821 ccagataggg cacgaacagt gaaagcacca ccttctcggc ggcctcgaaa ccgccgtcca
     5881 gcaacaccgc cccgaacagc gcctccatcg catcggccag catggaaggg cgacgaaatc
     5941 caccgctctt gcgctcgcct tcacccagct gcagataact gccgagttcg agttgctgcg
     6001 ccagtccggc caaggtctct tccttgatca gattggaacg caagcgggtg agatcccctt
     6061 cgctcagttg cggataactg aggtacaggt gcttggcgat gacgcatccc agcacgctgt
     6121 cgcccagaaa ttccagccgt tcattgtgct ccggcgcaga actgcgatga gtcagcgcgc
     6181 gctccagcaa ctggggctgc gcaaagacat gccccaactg cttgctgagc gtccgtctgt
     6241 ccctacttgc cactggatgg attgaattcg aggtacaggc tgatgttggc gaccagcggt
     6301 atcttgacgg cataactggc gctcagcttc aacactccgt tgtccttgct gatttcgatg
     6361 tcgtcggggg tgatcgcccg gatatcctcg accgaggcgc gacggatata agacatcttg
     6421 atcgagccca cattggcccc ctgcatctcc ggatcgtggg caacggtaac gaacagattc
     6481 ttgatcgttt cgttctgcat ataggccggg atcagcttca gcccggtgat gctcaagagc
     6541 acgacgagga acgcgccgaa gataaacccg aaaaaggtca aaccccgctg ttgcgccggc
     6601 attgctgtat tcatcttgtt tccccctcag ttaatagaca gaccgatccg tttcagatcg
     6661 gagaaattca tccagataaa aaacgccttg ccgacgatct gatggtccgg cacaaaaccc
     6721 cagtaacggc tgtcgcggct attgtcgcgg ttatctccga gcatgaagta ctggccctca
     6781 gggaccttgc aacgcaccat ctcatcgtcg tagctgcatc cctctcgccc cttgaactcg
     6841 gcgaccgaac tcagatgcaa ggtcggcatc tcgggattga ccagcaccgc gtgcttgtgt
     6901 tcacccaggg tctcgatgcg tttctcggtg tgcacgaagc tcagcccgga ctcgacgtaa
     6961 ttgtaatcgc catcggactt ttgaatctgt tcgacgccat tgacccacag ccgcttgtcg
     7021 cggtattcga ccgtatcgcc tggcaatccg accacgcgct tgatgtagtc cagcgaagga
     7081 ttttccggat agtggaacac catcacgtcg ccgcgctgag gatcgttgat ctgcacgatc
     7141 ttctggttga tgataggcag gcgaatgccg taattgaatt tattcaccag aatgaagtcg
     7201 cccacctgca gggtgggaat catcgatccg gacgggatct tgaacggttc gaccagaaac
     7261 gagcggataa agaacaccac cagtatcacc gggaagaagc tcttggcgta ttccacccac
     7321 cacggctcgg caactccgtc ggcgcgcctg cccgccagcg caaaacgatc cagcaaccac
     7381 acgctgccgg tcaccagcag cagcaagaac aatatcagcg cgaaatccat cgttacccct
     7441 atttatcttc gacgcgcagg atggcgagga aggcttcctg cggaatctcc acgttgccca
     7501 cctgcttcat gcgcttcttg ccggccttct gtttctccag cagcttcttc ttgcgcgaaa
     7561 tgtcgccgcc gtagcacttg gccagcacgt tcttgcgcat cgccttgacg ttttcgcgcg
     7621 cgataatgtt cgaaccgatg gtcgcctgga tcgccacgtc gtacatctgg cgggggatca
     7681 gctcgcgcat cttggccgcc acttcacgcc ctcggtactg gctgttcgca cggtgtacga
     7741 tcaccgccag cgcatcgacc ttgtcgccgt tgatcagcat gtccaccttg accacgtcgg
     7801 ccgggcgata ctccttgaac tcgtaatcca tcgaggcata gccgcgcgat acggacttca
     7861 gcctgtcgaa gaaatccagc acgatctcgc ccatcggcat ctcgtagatc agcttcacct
     7921 gcttgccgtg atagttcata tcgatctgta caccgcgctt ctgcgtgcat agcgtgatca
     7981 ccgagccgac gtattcctgc ggcatgtaca ggttcaccgt gacgatgggc tcgcgtattt
     8041 cctcgatctt cgacgggtcc ggcagcttgg acgggttatc caccatcagc accgtaccgt
     8101 cgcgcagcac cacctcgtag accaccgtcg gcgcggtggt gatgagatcc atgtcgaact
     8161 cgcgctccag ccgctcctgc acgatctcca tgtgcagcag gccgaggaag ccgcagcgga
     8221 agccgaagcc cagcgcctgc gagacttccg gctcgtacat cagggccgca tcgttcagtt
     8281 tcagcttctc cagcgaatcg cgcagcgcct cgtactggtt ggattcgacc gggaacaggc
     8341 cggcaaacac ctgcggctgg acttccttga aaccgggcaa tgccgatgac gcgggcctgc
     8401 ccgccagcgt gacggtatcg ccgaccttgg catctttcag ttccttgata ccggcaatca
     8461 cgaatccgac ctgcccggca gacagcgatt cgcgatcctg agccttgggc gtgaacacgc
     8521 cgatatgctc ggtcagatgt tgcgcgccgg tggccataaa caggatcttc tccttgggct
     8581 tgagcgtgcc gttgacgatg cgcaccagca tcaccacgcc gacatagttg tcgaaccacg
     8641 agtcgatgat cagcgcctgc aagggggcat ccggatcgcc tttgggagcg ggcaccttga
     8701 cgatcagcga ctccagcaca tcttccacgc cgagtccggt cttggcggaa caatgcaccg
     8761 catcgtgcgc gtcgatgccg atcacatcct cgatctcggc gatggcgttg tcgggatcgg
     8821 cggacggcag atcgatcttg ttcagcaccg gcaccacttc cacgccgaga tcgagcgcgg
     8881 tatagcagtt agccacggtc tgcgcctcca ccccctgcga ggcatccacg accagcagcg
     8941 cgccctcgca ggcggacagt gagcgcgata cctcgtagga gaagtccaca tgtcccgggg
     9001 tgtcgatcag attcaggttg tagacctgac catcgcgcgc cttgtagctg agtgccgcag
     9061 tctgcgcctt gatggtgatg ccgcgttcgc gctcgatgtc catcgagtcg agcacctgcg
     9121 cctccatttc acgatcggac aaaccgccgc atagatgaat gatgcgatcc gccagcgtgg
     9181 atttgccgtg gtcgatatga gcgatgatgg aaaagttacg gatatgttgc atggaggtcc
     9241 agataaatgc ctaaatcggc tgattcgcaa agcgcggatt ctagccgatt taggcgatgc
     9301 ccgcaggggc gcgattactt ctcgtcgagc ctgatcgcca cataagaggc cgcatcgccg
     9361 cgccgcacca gcagcgcgac gttacgcccc ttgggcacct gcttgagaat atcgttgaac
     9421 tgggccagcg agcgtatcgg catattgcct acggccagca acaagtcacc gcgttgcaat
     9481 ccagccgcat gcgcagcctg ccccttgacc tgttccacca gcaacccgcc gacaacgtcc
     9541 agctcctgca actgctccct gctcagctcg ctgaccgcga tccccagacg cgagatcatc
     9601 tcggctacat cgtcttccag cttcttcacc gcctgcgcca gtcggctttc ctccggcatc
     9661 tccgcgatca ccacggtcac cgagcgggac gcgcccttgc gccacagctc catgacggcc
     9721 ttgctgcccg gcttggtcgc ggccaccagt cgcggcaagt cagcggaact gtttaccgcc
     9781 ttgccgtcga atttgagaat cacatcgctg gcctcgatac ccgccttgtc tgcgggacca
     9841 ttcttttcaa cagaactgat caacgcacca ttgggcttgc tcaagccaaa ggagtcggcc
     9901 aattcccgcg tcagctcctg tatcgtcacg ccgatgcgac cgcgcgtcac cttgccggta
     9961 gtccgcaatt gatcggtcac ctgagtcgcc acgtcgatcg gtatcgcgaa ggacaagccc
    10021 atcgaaccgc cggaacgggt ataaatctgc gagttgatgc cgaccacttc gccgttcatg
    10081 ttgaacagcg gaccgcctga attgcccgga ttgatcgcca catcggtctg gatgaaaggc
    10141 acgaagttgt cttgcggcaa agagcgcccc ttggcactga cgatacccgc cgtcacgctg
    10201 ctatcgaaac cgaacggcga accgatggca accacccatt cccccacctt caatttatcc
    10261 gggctgccca gactcacctg cggcagaccg gccgcctcga tcttcaacag cgcgacatcg
    10321 gtgcgccggt cggcgccgat caccttggcg ctatattcgc gcttgtcggt cagccgcacg
    10381 gtgatctttt cggcatggtc caccacatgc gcattggtca tgatgtaacc gtcggcgctg
    10441 atgatgaagc cggaacccag cgactgcgat tcctgctcgc gcggtgcctg tccttgcggc
    10501 atgggggggg caaaacggcg aaagaattcg aagaacggat cgccttcctg cagacccgga
    10561 aatcctggcg cattgcggat gatttgcgtg gtgctgatat tgacgacggc agggccctgc
    10621 ctttcgacga gatcggtaaa atccggcaat tcgcgcgcct gcaccgcagg tgacagcatc
    10681 agccccatag ccaacaagac gaaaaacttt ctcagcatgc gttcctcctc ataatgaaaa
    10741 aattgacacc tgcgacacct agccctcgcg ctcctgccgc acgatgcgcg gcagctgacg
    10801 ggattgccgc ttatccagcc accgggacac gataaatccg agcaccaacc ccagcgcagc
    10861 gcctgccgca gcataaccgt cgcgctgctc gtccagagtc gcgatcgcgt taccgcacgc
    10921 cgcgcccgcc accagcagcg acagcggcaa cagatagacc aagccgatgc cgcgcagcac
    10981 ggcgccgtcc gcaaccgaca ccacgacctc atccccgact gccgcgccga tcgcattgtc
    11041 cacctggaat tgacgcggct cacggcaaag caagccggac agcttgcctg caccgcaccc
    11101 cgtacttttg cattgtccgc aaccgctggc ctgagcactc tcgacgaacg caagcggccc
    11161 atcgattctc accacgatag cgcgagtctc cagcgtcatc gcgtaccgtt gtaacgcaac
    11221 gaatcgatca cctgcatcac ggtacgtccc ggcacctctc cgaccacggt gaacagatgt
    11281 ccgtccgcaa ccttgtggta gagattcatc gccccgcgac tggacaatcc ggcactgtcg
    11341 tcctcgtcct tgtcattcgg ctcgataaag acagagatcg cgctcagccc atcggaaaac
    11401 accagctgca ccaccggggc gtgcctgcca cgcatgggac gctgaatctc catggtcttc
    11461 ttgaagcctt ccggcaatgc gtcgaccacc caaccgctgt tcacaggcac gccgttgccc
    11521 gctggcgagg catcgcccgc gatgcctccc gtcgtcgcac tggctaacgg cgcggatttg
    11581 atccaggaac gatcgatgcc ccccccaatt tgcaattggg taaaagcgta ttgttcaacg
    11641 ggttgatttt tatcccccag caccacggtc ttcagcaaca aaccggagtc cgtatgcacc
    11701 caaagcttgt ggttgtaacg caactgatcc ttgggctgaa acagaatgac ctgagcatcg
    11761 aaccccgcga tccgctccac cccgacctgt ttggcttggt aattcagatt cagcgcagac
    11821 atctgctccg gcaacagcga ggggaatcga ccgcggcctt gctgcccgac atacaccatc
    11881 ttgtgattgc tgtattccca gatatgcccg tgatgacgga tgatttcccg cttcggaccg
    11941 tccatgcact cgagcttttc gtattctccg tccgcctcga ccacatgagt gatacgcgag
    12001 gtctctaccc ggttgtcata ttggtagatg aacacaccgc tgtaatcggt ctggtgaccg
    12061 gcaaaggcca ccgttttgag ccagtccatg ctgacggctc cgccctccgc ccgggcattg
    12121 cccatggaca gcaacaaaca gaggactgca acacgcccct tcatcgtcac ttgccctcgg
    12181 gacggtagga aacggtacgg agataagaag caccgccgct catatctgca ctgggcgaga
    12241 attcctgatg cgccatcaga taatcattgg ccttgggctg tatctgcgca ctcgcaggac
    12301 gcaagccgcc ctgctgcatg gccagttgcg gcgccgcctc aggactgatc tgcagcgaca
    12361 tccaggacac cacgccgacc gccagcaacg atgcggcggc agacaaggca aaggcgcgca
    12421 ccttctgctt ggaggcccgc acgcgcggcg ccagcaccac aggctcatcc tgcatacgtt
    12481 cacgcacctt cgcgctcagg tcgcaatgga tgtgttcggg ttggcgcaac acatcaccga
    12541 tcaggtgata aatttcccag tccttgcgtg cattggcgtc gcgtctgact tggccgaaca
    12601 aatcccctgc ctcatcttca aacaactcgc catccatcaa agtcgaaata ttctgtttca
    12661 tatctaccac ctgttacctt tgctggtttc taacaacgga cgcagatttt ctgccactgc
    12721 ctcccgcgcc ctgaaaattc tcgatctcac cgtgccgatc ggacaattca tcacactggc
    12781 gatctcttcg taactcaatc cttcgatctc tcgcaacgtc agcgcactgc gcaaatcctc
    12841 cggcaactgc tgcaacgagg cctgcaccac atcgaccacc tgcttgctca tgagttcatt
    12901 ctccggcgtg ctcacctcgt gcagcaggct ggcctcctcg aacgactccg catcatcggt
    12961 atcgaacagc gtgctggtcg gcgcacggcg cttgttcgcc agcagatgat tcttcgccgt
    13021 attgatgccg atacgataca gccaggtata gaacgcgctt tcgccgcgaa aacccggcag
    13081 agcgcgatac gccttgatga acgcatcctg cgtcacatcc tcggcctcgg ccgggtcgcg
    13141 cacgaaccgc gaaatcagcc gcccgagtct gcgctggtat ttggaaacca gcagatcgaa
    13201 ggcatgctta tcgccgcgct gaacgcgttc caccaacaat tgatcgactt cccgatccgc
    13261 catccctgaa ttattccgat tattattttg actgccgcct gcatcgcggg gcgccagtat
    13321 aaccgctccg ctttgcccgc gtcagcatga cggactatgc gaagaatgcg acccggcaag
    13381 accaatttag ttccatgaca agcaaacgac cgctgccaga ggccactctt ttgctatagt
    13441 tccaactcgc aaccctactc aggacaagca agtgctgaaa ttcgacacgt tgatcatcgg
    13501 cagcggcctc gccgggctga cgctagccct caaactggcc gatcaccagc gcgtcgggct
    13561 aatcacgaag aaatccctgc aggacggcgc cagcagctgg gcacaggggg gaatcgccgc
    13621 cgtgctgtcc gagaacgaca gcatcgatgc gcatattcag gacacgctca tcgcaggtgc
    13681 cggattgtgc gacgaggctg ccacacggca tgtagtcgaa cacagcaagg ccgccatcga
    13741 ctggctgatc gcacagggcg tgccgttcac ccgcgacgat accagcaaca tgggatacca
    13801 cctcacccgc gaaggcgggc acagccagcg ccgcatcatc cacgcggccg acgcgaccgg
    13861 tcacgcggtt caggaaacac tgtccgcccg cgtactgcgc catccgaaca tcaccgtgct
    13921 ggaaaaccac atgtcggtcg acctgatcac cggcaagaat ctgggacaga ccgacaatcg
    13981 ctgttatggt gcctatgcgc tgaactgcct gagcgacgag gtcatcaccg tcgtcgcgca
    14041 aaacaccata ctggcgacgg gaggcagcgg caaggtttac ctctacacca ccaacccgga
    14101 caccgcaacc ggtgacggta tcgcgatggc ctggcgcgcc ggctgtcgca tcgccaatat
    14161 ggaattcatt cagttccacc cgacttgctt gtatcacccg catgccaagt ccttcctgat
    14221 cagtgaagcg gtgcgtggcg aaggcggcat attgaaactc ccggacggca cgcgcttcat
    14281 gcccgcccac gacgaacgcg ccgaactcgc gccacgcgat gtggtcgccc gggccatcga
    14341 ctacgagatg aaaaagcacg gtctggactg cgtctatctg gacatttcac accagtcggt
    14401 cgaattcctg caggaacact tcccgaccat ctacgcgcgc tgcctcaagc tgggcatcaa
    14461 tatcagccgg gagccgattc cggtcgtgcc ggcagcgcac tatacctgcg gaggggtcat
    14521 gaccgacctg catgctcgca ccgacgtaga gggcctgtat gccgtagggg aaacggccta
    14581 cagcggcctg catggcgcca accgcttggc cagtaactcg ctgctggaat gcctggtatt
    14641 cgccgacgcg gcgatacgcg atattctgga caaaccgcat ctcacgccac cgcccctgcc
    14701 cgcctgggac gagagccgcg tcaccgacgc ggacgagcag atcgtcatct cgcacaactg
    14761 ggcggaactg cgtcacttca tgtgggatta cgtcggcatc gtgcgcacca acaagcgtct
    14821 gcagcgagca cagcaccgca ttcaattgct gcaggacgag atcaacgagt actacaccaa
    14881 ttttcacatc agcccggatc tgctggagtt gcgcaatctg gtgatgaacg ccgaactgat
    14941 cgtgcaaagc gccctgctgc gccatgaaag ccgcggcctg catttcagca aggattatcc
    15001 agagaccctg cccaatccac agccgaccat cctcacccca ccgccccgct aacccagtct
    15061 cagacggaca cacaggtcgc gaaagacatc gcgctccagc gcatccggga agatgacgcg
    15121 cgataccagc cagcgcctcc cctctatcct gaggcgcaac aacacgaaat aagggctgac
    15181 gaaggttcca tccacgacat ttccgatcat gacgccagcg tcccggcgaa ggactgaaac
    15241 ctcgccctgc ttgacggaca acccacacca ggagtcaggc agaagcacga gggcgtcacg
    15301 cgccagatgg tagagaacgc tgagcagcaa cagcgggaat atcacgatct tgaacgcaga
    15361 gggcatgacc gtgacatacg ccgctccggc tgcaagcgaa tgcgaaaaca acagtaagaa
    15421 gacgaagcgc ggcgaaggat ggatcgccat ttgctccgcc ggggaatcag atcacgaaga
    15481 aaacaatcag gcccagcaac gccagcacga tgatggccag cagcagcccg ccaccggatc
    15541 gcgagacctc cctgtcctga tgcgcggcaa caggaggcaa cttggcggcc ttgtttaccg
    15601 ggggaggcgc cgcccgatta attgccccga tggcgggtcg agccattttc aaggtcttgc
    15661 tcaactcgtc cacatcctct gagcttgcgg tcatggcggc aagcttcatc gttgcggcac
    15721 cggaatcaaa agcctgcgac agcttcgccg tcgaggcggc tcccacttct tcggcttcga
    15781 cgtgcctgcc gccggttacc gagatcgcat ccggcaattt cttttcgcct cccgccggtc
    15841 tcttggacac gtctatcggc tgaccggaac gaggcaaggt atccctgcag gcccgcaaat
    15901 cggcagcaaa atccttggcg ttctggtagc gatcctccac cgacttcgcc agcgccttgg
    15961 ccacgatgaa atcgatcatc tcgggagcgg acggattgac ctgactcggc ggcggcgggg
    16021 ccgcattcaa ggtctggtac atgatggcgt tgacattctc gccactgaat ggcgcctgcc
    16081 cagtcagcat ttcatagagc atcacgccca gcgagaaaat atccgagcgt tggtcgatgt
    16141 tcttacccat cacttgctcg ggcgacatat atttcgggga tcccagcacc atgccggtct
    16201 gggttcgcac cgcagcggaa gccatacgcg cgataccgaa gtcggtgatc ttcacatgac
    16261 cgtcgcgcac caccatgata tttgagggtt tgacatcgcg atggacgatc tcatgttcgt
    16321 gggcataggc caagccgagc gcgacctgcg cgacgatttc cagcacctgt tcaaccggca
    16381 gccgctcgcc atcgttcatg atgtcgcgca attcgcgccc ttgcaggaac tccatcgcga
    16441 tgtaggcaac atcgccactc ttgcccacat cgtagattgt gacgatgttc ggatgattca
    16501 gccgtccggc ggccttggct tcctgataga agcggccctc gtactcttcc ttttcatcca
    16561 gcgccagacc caagttaatg gtcttgatcg cgacgatacg gtcaatcagc gggtctttgg
    16621 ccttatagac cacgcccatc gcgccctgac caagctcgcc cagcacctca tatcgcccca
    16681 actggctaat catggcactc gtccgaaaaa ttcttcatta tgcggaaccc aagtttgatg
    16741 catctgctca ctacctgcag aaacccggct tatagacacg cgccgtttta cccgattcga
    16801 catcaacttt ctgcgtataa ataccaccgc tcgagtgact cagaatcagg ctatgctgcc
    16861 ctggcgaaac gctcaaagtc atgacgccgc catctgtcat cttgcccttt tgcgtacc
//
LOCUS       DASUIV010000055         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_13710, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000055 DASUIV010000000
VERSION     DASUIV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13710"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFW59_01840"
     CDS             <1..374
                     /locus_tag="VFW59_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5362986.1"
                     /translation="IATKEFIGEKGVLKAIKAVRVEWKDGKMSEIAGSEFELKADLVF
                     LAMGFTNPLQQVLDAFGVEKDARGNVKADTEDYRTSVDKVFAAGDMRRGQSLVVWAIR
                     EGRQCARAVDEFLMGSSVLPR"
     gene            441..1505
                     /gene="hemE"
                     /locus_tag="VFW59_01845"
     CDS             441..1505
                     /gene="hemE"
                     /locus_tag="VFW59_01845"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX5362987.1"
                     /translation="MNFKLKNDTFLRALLRQPTEYTPMWMMRQAGRYLPEYRETRKQA
                     GSFLGLCKNPDYATEVTLQPLERFPLDAAILFSDILTVPDAMGLGLYFSEGEGPKFER
                     PLKTEADIMALRVPDIGKDLRYVTDAVTQIRRALDGSVPLIGFSGSPFTLSCYMVEGG
                     GSDDYAKVKTLMYNEPKLMHHILEVTTQAVIAYLNAQIEAGAQAVMIFDSWGGVLSHA
                     AFHEFSLAYTQRIIDGLIKEKDGVRIPSIVFTKGGGLWIENIANTGCDAVGLDWTMDI
                     GIARQKVGHKVALQGNMDPAVLFASPEAIYKEVDRILGSYGYGSGHVFNLGHGISQHT
                     NPEHAAALVAAVHELSRKYH"
     gene            complement(1587..2462)
                     /gene="ubiA"
                     /locus_tag="VFW59_01850"
     CDS             complement(1587..2462)
                     /gene="ubiA"
                     /locus_tag="VFW59_01850"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX5362988.1"
                     /translation="MTRPTSPTERLTFYIHLTRLHRPIGILLLLWPTLWGLWIAGEGH
                     PAWHIVLIFALGTTLMRSAGCAVNDYADRHIDKHVKRTKDRPLTSGKVSERETLWLAV
                     ALTLAAFLLILPLHPLTWLLSFPAAFLAASYPFTKRFFAIPQAYLGIAFGFGIPMAFA
                     ATLGHVPAVAWVLLLANVFWAIAYDTEYAMVDRDDDVHLGIHSSALLFGKYDVAAVMT
                     CYAITLGLLALAGQMVSLGWMYDAGLAMAAGIALYHYTLIRNRNRERCFAAFLHNNWI
                     GAAVFAGLFANYALR"
     gene            2618..3004
                     /locus_tag="VFW59_01855"
     CDS             2618..3004
                     /locus_tag="VFW59_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5362989.1"
                     /translation="MQQLTPKQAYEFLQHNPEAIFVDVRSEMEHMFVGNPKGSILIPW
                     VDGPEWDINPHFVSQVRKAASQNRPVVLICRSGRRSEDAGLALEKDGFTEVYNVTHGF
                     EGDLDDSHHRNSHNGWRFDGLPWGQT"
     gene            3527..>3783
                     /locus_tag="VFW59_01860"
     CDS             3527..>3783
                     /locus_tag="VFW59_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362990.1"
                     /translation="MKEFSIDLAAEQIADQRTKEYFQEVLGSFINGNYRSAVVMLWSV
                     VVADFVYKLQALRDLYQDPTAISILASIEEKQQANPTSPEW"
BASE COUNT          794 a         1064 c         1178 g          747 t
ORIGIN      
        1 cgatcgccac caaggagttc atcggcgaga agggcgtgct caaggccatc aaggcggtgc
       61 gcgtcgagtg gaaggacggc aagatgagcg agatcgcggg cagcgagttc gagctgaagg
      121 ccgatctggt gttcctcgcg atgggcttca ccaatccgct gcagcaggtg ctggacgcgt
      181 tcggcgtgga gaaggatgct cgcggcaacg tcaaggccga taccgaggac taccgcacca
      241 gcgtggataa ggtattcgct gctggcgaca tgcgtcgcgg ccagtcgctg gtggtctggg
      301 caatccgcga aggtcgccag tgtgcccgtg ccgtagacga gttcctgatg ggctcgtcgg
      361 ttctgccgcg ctagtcgaca agggcgggcc cgttcatata acgggtccgt ccgtataaaa
      421 aaacaaatca gggaaacaaa atgaatttca agttgaagaa cgataccttt ctgcgtgcct
      481 tgctgcgcca gcctaccgaa tataccccga tgtggatgat gcgtcaggcc ggccgctatc
      541 tgccggaata ccgcgagacc cgcaagcagg cgggcagttt cctcggcctg tgcaagaacc
      601 ccgactatgc gaccgaagtg acgctgcagc cgctggagcg cttcccgctc gatgctgcga
      661 tcctgttctc cgacattctc accgtgccgg atgcgatggg tctgggtctg tacttctccg
      721 aaggcgaagg cccgaagttt gagcgtccgc tgaagacgga agccgacatc atggcgctgc
      781 gcgtgccgga tattggcaag gatctgcgct acgtcaccga tgcggtgacc cagatccgcc
      841 gtgcgctcga cggcagcgtg ccgctgatcg gattctccgg cagcccgttc accctgtcct
      901 gctacatggt cgaaggcggc ggcagcgacg attacgccaa ggtgaagacc ctgatgtaca
      961 acgagcccaa gctgatgcac cacattctgg aagtgaccac gcaagccgtg atcgcctatc
     1021 tgaatgcgca gatcgaagcg ggcgcacagg ccgtgatgat tttcgattca tggggcggcg
     1081 tgctgtcgca tgcggcattc catgaattct cgctggctta cacccaacgc atcatcgatg
     1141 gcctgatcaa ggaaaaagac ggcgtgcgca tcccgtccat cgtgttcacc aagggcggcg
     1201 gtctgtggat cgagaacatc gccaacaccg gttgcgacgc ggtcggtctg gactggacca
     1261 tggacatcgg catcgcgcgt cagaaggtcg gtcacaaggt tgcgctgcag ggcaatatgg
     1321 atccggccgt gctgtttgcc tcgccggaag ccatctacaa agaggtcgat cgcatcctcg
     1381 gcagctacgg ctacggcagc ggtcacgtgt tcaatctggg tcatgggatc tcgcagcaca
     1441 ccaatccgga gcatgcggcg gcgctggtcg cggcggtgca cgagctgagc cgcaagtacc
     1501 actagcagct ttatcgctga aacgaaaaag ccgccggatg ctctccggcg gctttttgta
     1561 tttcgtggac aggtgagcgg tggggtttat cgcagcgcgt agttggcgaa cagtccggcg
     1621 aataccgcag cgcctatcca gttgttgtgc aggaacgcgg caaagcagcg ttcgcggttc
     1681 ctgtttctga tcagtgtgta gtgatagagc gcgatgcctg ctgccatcgc taaacctgcg
     1741 tcatacatcc agcccaggct caccatctgt ccggccaatg ccagcaggcc cagcgtgatt
     1801 gcgtaacagg tcatcaccgc cgccacgtcg tattttccga acaatagcgc cgaggaatgg
     1861 atgccgagat gcacatcgtc gtccctgtcg accatcgcgt attcggtgtc gtaggcgatg
     1921 gcccagaaca cgttggcgag cagcagcacc caggcgaccg caggcacgtg gcccagcgtc
     1981 gcggcgaagg ccatcgggat gccgaagccg aaggcgatgc cgagataggc ctgcggaatg
     2041 gcgaagaagc gcttggtaaa cgggtagctg gcggcgagaa aggcggcagg gaaggacagc
     2101 agccaggtca gcggatgcag cggcaggatc agcaggaaag ctgccagtgt cagcgcgacg
     2161 gccagccaca gcgtctcgcg ttcgctgacc ttgccgctgg tcagcggcct gtccttggtg
     2221 cgcttgacgt gcttgtcgat gtggcggtcg gcatagtcgt tgacggcgca accggcggag
     2281 cgcatcagcg tggtgccgag tgcgaagatc agcacgatat gccaggccgg gtgaccctct
     2341 cctgcgatcc acaggcccca cagcgtcggc cacagcagca gcaggattcc gatagggcgg
     2401 tgcaggcggg tcagatggat gtagaaggtc aggcgttcag tcggtgaagt cgggcgggtc
     2461 atcgggtgct tgtgtttgta cgcaggcagg caggatatgt cggcggcgca taaaattcaa
     2521 tgaatgaagg tgcgggctca acacaggctg gacggacaac gcgctggggc actatcatcg
     2581 cgtccgctcg ccagacaggg cgaattgagg agacaacgtg cagcaactga cacccaagca
     2641 ggcttacgaa ttcctgcaac acaatcccga agcgatcttc gtggatgtgc gcagcgagat
     2701 ggaacacatg ttcgtcggca atccgaaggg ttcgatcttg attccctggg tggacgggcc
     2761 ggaatgggac atcaatccgc acttcgtctc gcaggtaaga aaggcggcca gccagaatcg
     2821 cccggtcgtg ctgatctgcc ggtccggtcg gcgttccgag gatgccgggc tggcattgga
     2881 aaaagacggg tttaccgagg tctacaatgt gacccatggt ttcgaaggcg acctcgacga
     2941 tagccatcac cgcaattcgc acaacggctg gcggttcgac ggattgccgt ggggacagac
     3001 ctaagcctac tgcgcgacac gattgcggcg ttgcgcggtg ctcggaatcc tcatgtacat
     3061 tgagtacatt ccggttcctg cgctccgtgc gccttgcact cgagccgctc gctacggctt
     3121 accttttatg atcggttaaa aacaaaaccg tcgggggtag cccgacagct agttactttc
     3181 ttttgcttcg ccaaaagaaa gtaaccaaag aaaaggcgac cccggttcgc cgcccgcttc
     3241 gcgggttccc tgcgttgctc ggccggtcag gcggctgcgg aactcgcgct acgcgctcag
     3301 acagtcctcg ccgaaatccc ctgaccgacc tccgctactc ggcggctctc aggggaaatt
     3361 gaaaaacaag tggtgtgggc gcagcgcgcc ctcgttattt ttttgagtaa gcaatggcgc
     3421 aaaccgccat cgctcacagg tcgcctatat atttcgttat atggttttcc ggctggattg
     3481 ctgttagggt tcgctccact ttactagaca tcaccacgag catcctatga aagagttctc
     3541 gattgatctt gcggctgaac agatcgctga ccagcgaacc aaggaatatt ttcaggaggt
     3601 actgggctca tttattaacg gaaactacag atccgccgtt gtcatgcttt ggtctgttgt
     3661 ggtcgcagat ttcgtttaca agttgcaggc attgcgcgat ctctatcaag atcccactgc
     3721 gatttcaatt ctagcctcaa ttgaagagaa gcagcaagcg aatccgacga gtcccgagtg
     3781 gga
//
LOCUS       DASUIV010000056         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_479279, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000056 DASUIV010000000
VERSION     DASUIV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479279"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFW59_01865"
     CDS             complement(<1..418)
                     /locus_tag="VFW59_01865"
                     /inference="COORDINATES: protein motif:HMM:NF020794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEX5362991.1"
                     /translation="MAKHKGMTSEQFLQHLQSHAESFAAAVATNEGDWIIKGFIDVYQ
                     RIYTISVDTKIVSKVLELLLFPMFVEFGKQHGLEVELSPHQNFYPDLTFVDKKTGNKF
                     AVDIKSTYRVDDANVNGMTLGAFTGYFRNRKSRKNTL"
     gene            complement(421..1734)
                     /locus_tag="VFW59_01870"
     CDS             complement(421..1734)
                     /locus_tag="VFW59_01870"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5362992.1"
                     /translation="MKPINVGLLGIGTVGGGTFTVLARNAEEITRRAGRPIRIVAVAD
                     KNVELAKQVTGGACRVTDDAFSVVSDPEVDIVIELIGGYGVAKELVLKAIANGKHVVT
                     ANKALLATHGNEIFKAAQDKGVMVAFEAAVAGGIPIIKAVREGLTANRIEWIAGIING
                     TTNFILSEMRDKGLSFDTVLKEAQRLGYAEADPTFDIEGVDAAHKITILASLAFGIPM
                     QFDKAYIEGISRLDAIDIKYAEQLGYRIKLLGITKRTDEGVELRVHPTLIPTKRLIAN
                     VEGAMNAVLVQGDAVGATLYYGKGAGAEPTASAVIADLVDVTRMATSDPENRVPHLAF
                     QPNAMADLKILPMDDVITSYYLRLRVQDKPGVLADITRILADEQISIDAVIQKEPGEG
                     ETQADLIMLTHQTREKRIVAAIAKIEALGVVAGKVTKLRMEELGK"
     gene            complement(1827..3065)
                     /locus_tag="VFW59_01875"
     CDS             complement(1827..3065)
                     /locus_tag="VFW59_01875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5362993.1"
                     /translation="MKPILKSTKLSNVCYDIRGPVMERAKQMEEEGQRIIKLNIGNLA
                     PFGFEAPEEIQQDVILNLPNSSGYSDSKGMFAARKAIMHYCQGKNIKGVGLDDIYIGN
                     GASELIVMAMQGLLNTGDEVLVPAPDYPLWTAAVTLAGGTPRHYICDEASEWMPDLAD
                     MRSKITPNTRAIVVINPNNPTGALYSDEMLMEIIQIAREHQLIIFADEIYDKMLYDGA
                     THTSIASLADDVLFATLNGLSKNYRACGYRAGWMVLSGEKKHAQDYINGLTILASMRL
                     CSNVPGQFAIQTALGGYQSINDLVAPTGRLCKQRDLAYELITAIPGVTCVKPKAGLYL
                     FPRFDPKIYPIVNDQQFILELLEAEKVLVVQGSGFNWIHPDHIRIVFLPIADDLADAM
                     GRIARFLENYRKQHGTEISR"
     gene            3224..3604
                     /locus_tag="VFW59_01880"
     CDS             3224..3604
                     /locus_tag="VFW59_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX5362994.1"
                     /translation="MKLHLTRLDDTKMFTAHAPDHVTVNGERFDTSIVVRAAEVRGDW
                     NVAAFDALSEADFDYFLALKPEVLLFGTGTRQRFAHPRLYRALTDARISVEFMDTPAA
                     CRTYNILVAEDRNVVAAILVEDPA"
     gene            3601..>3828
                     /locus_tag="VFW59_01885"
     CDS             3601..>3828
                     /locus_tag="VFW59_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5362995.1"
                     /translation="MNHTLNNVITQAEQVILGKPRQIRMALACLLARGHLLIEDLPGV
                     GKTTLAQVLARTLGLQFQRVQFTSDLLPADIL"
BASE COUNT          703 a         1180 c         1112 g          833 t
ORIGIN      
        1 atagcgtatt ttttctgctc ttgcgattac ggaagtatcc agtaaatgcg cctagtgtca
       61 tgccgttcac gttcgcatca tctactcgat aggtactttt tatatcgaca gcgaatttat
      121 ttcccgtctt cttgtcgaca aacgttaggt ctgggtaaaa attttgatgc ggtgacaatt
      181 cgacttccaa accatgctgc ttaccgaact caacgaacat tggaaaaagc agtagctcaa
      241 gaaccttgga aacaatttta gtatcaacag aaatggtata gattcgctga tatacgtcga
      301 tgaacccttt gatgatccag tcaccctcat tggtcgctac tgcggcagca aaggattcgg
      361 catgagattg caaatgttgc aaaaattgct ctgatgtcat gcccttatgt tttgccatga
      421 ttacttaccc aactcttcca tccgcagctt ggtcaccttg cccgccacca cgcccagcgc
      481 ctcgatcttc gcgatcgccg caacgatgcg cttctcgcgg gtctggtggg tcagcatgat
      541 caggtcggcc tgggtctcgc cttcgcccgg ttccttctgg atcaccgcgt cgatggagat
      601 ctgctcgtcg gccaggatgc gggtgatgtc ggccagcacg cccggcttgt cctgcacgcg
      661 caagcgcagg tagtagctgg tgatgacatc gtccatcggc aagatcttca ggtcggccat
      721 cgcgttcggc tggaaggcca gatgcggcac gcggttctcc ggatcggaag tcgccatgcg
      781 cgtgacgtcc accagatcgg cgatcacagc actcgccgtc ggctcagcgc ccgcgccctt
      841 gccgtaataa agcgtcgcgc cgaccgcatc gccctgcacc agcaccgcgt tcatcgcgcc
      901 ttccacattg gcgatcaggc gcttggtcgg aatcagggtc ggatgcacgc gcagctcgac
      961 gccctcatcg gtgcgcttgg taatgcccag cagcttgatc cggtagccca gttgttcggc
     1021 gtatttgatg tcgatcgcgt ccagcctgga gatgccttcg atataggcct tgtcgaactg
     1081 catcgggatg ccgaacgcca gcgaggcgag gatggtgatc ttgtgcgccg catccacgcc
     1141 ttcgatgtcg aaggtcggat cggcctcggc gtagcccaga cgctgcgcct ctttaagcac
     1201 cgtgtcgaag gacagcccct tgtcgcgcat ctcggacagg atgaagttgg tcgtgccgtt
     1261 gatgatgccc gcgatccatt cgatgcggtt cgcggtcaga ccttcgcgca ccgccttgat
     1321 gatggggatg ccgcccgcta ccgccgcctc gaacgcgacc atcacgccct tgtcctgcgc
     1381 ggccttgaag atctcgttgc cgtgggtcgc cagcagcgcc ttgttcgcgg tgaccacgtg
     1441 cttgccgttg gcgatggcct tgagcaccag ctctttcgcc acgccgtagc cgccgatcag
     1501 ctcgatgacg atatcgactt ccgggtcgga aaccaccgaa aacgcgtcgt cggtcacgcg
     1561 gcatgcgccg ccggtgacct gcttcgcgag ttccacgttc ttgtcggcga ccgccacgat
     1621 gcggatcggg cgaccggcgc gacgcgtgat ttcttccgcg ttgcgagcga gcacggtgaa
     1681 agttccgccg ccgaccgtgc cgataccgag tagtcctacg ttgattggtt tcataaaaca
     1741 gttcctagtc gttagtggtt agtcgttagt ctcgtgtcgt taatcgctag ctggcattgc
     1801 ccgctacacg ttttattaac tagcggctaa cggctgattt cagtgccgtg ttgcttgcgg
     1861 taattttcca ggaagcgtgc gatgcgtccc atcgcgtcgg ccaggtcgtc ggcgatcggc
     1921 aggaatacga tgcggatgtg gtccggatgt atccagttga aaccgctgcc ctgcaccacc
     1981 agcaccttct cggcctccag caattcgagg atgaactgct gatcgttcac aatcggatag
     2041 atcttcggat cgaaacgcgg gaacagatac aggcccgcct tcggcttgac gcaggtcacg
     2101 ccgggaatcg cggtgatcag ttcgtaggcc agatcgcgct gcttgcacag acgcccggtc
     2161 ggcgcgacca gatcgttaat gctctggtag ccgcccagcg cggtctggat cgcgaactgt
     2221 cccggcacgt tggagcacag acgcatcgag gcgaggatcg tcaggccgtt gatgtaatcc
     2281 tgcgcgtgtt tcttctcgcc ggagagcacc atccagccgg cgcgataacc gcaggcgcgg
     2341 tagttcttcg acagaccgtt aagcgtggcg aacagcacgt cgtcggccag cgaggcaatc
     2401 gaggtgtgcg tcgcaccgtc gtacagcatc ttgtcgtaga tctcgtcggc gaagatgatc
     2461 agctggtgtt cgcgcgcgat ctggatgatc tccatcagca tctcgtccga atacagcgcg
     2521 ccggtcgggt tgttcggatt gatgaccacg atggcgcggg tgttcggcgt gatcttgctg
     2581 cgcatgtcgg ccagatcggg catccattcg ctggcctcgt cgcagatgta atggcgcggc
     2641 gtgcccccgg ccagcgtgac cgccgctgtc cacagcggat agtcgggcgc gggcaccagc
     2701 acttcgtcgc cggtattgag caggccctgc atcgccatca cgatcagctc ggacgcgccg
     2761 ttgccgatgt agatgtcgtc cagccccacg cccttgatgt tcttgccctg acagtagtgc
     2821 atgatcgcct tgcgcgccgc gaacatgccc ttggaatcgg aatatccgga ggaattcggc
     2881 aggttcagga tcacgtcctg ctggatctcc tcgggcgcct caaagccgaa cggggcgagg
     2941 ttgccgatgt tcagcttgat gatgcgctga ccttcttcct ccatctgctt ggcgcgttcc
     3001 atcaccgggc cgcgaatgtc gtagcacacg ttggagagct tggtcgattt caggatgggt
     3061 ttcacgtttg ctcactcaca attgtttcgg gctcaatttg tccagggcat cgcgcgtccg
     3121 ctcagtgata gaatccggcg caaccacgca attaccgcaa ggggcgggat tttacctgcg
     3181 cagctccctt gcggcaaatg ccaaccgcaa atggagaatg gccttgaagt tgcatctgac
     3241 ccgtcttgac gacaccaaaa tgttcaccgc gcatgcgcct gaccacgtga cggtcaacgg
     3301 cgaacgcttc gacaccagca tcgtggtgcg cgccgcagag gttcgcggcg actggaacgt
     3361 cgccgctttc gacgcgctga gcgaagccga tttcgactat ttcctcgcgc tcaagcccga
     3421 agtcctgctg ttcggcaccg gcacccggca acgcttcgcc cacccgcgcc tgtaccgcgc
     3481 gctcaccgac gcgcgcatct ccgtcgaatt catggacacc ccggccgcct gccgcaccta
     3541 caacattctg gtggcggaag accgcaacgt ggtggcggca atactggtcg aggacccagc
     3601 atgaaccata ccctgaacaa cgtcatcacc caggccgagc aggtcatcct cggcaagccg
     3661 cgccagattc gcatggcgct ggcctgcctg ctggcacgcg gccatttgct gatcgaagac
     3721 ctgccgggcg tgggcaagac cacgctggcg caggtgctgg cgcgcacgct ggggttgcag
     3781 ttccagcgcg tgcagttcac cagcgacctg ctgcccgccg acatcctc
//
LOCUS       DASUIV010000057         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_107317, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000057 DASUIV010000000
VERSION     DASUIV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107317"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFW59_01890"
     CDS             <1..197
                     /locus_tag="VFW59_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5362996.1"
                     /translation="FTWQDKKGNQLPVCTGDFDFGCSCFDFATLRKQPTIPPSRLTAT
                     PLPFKFHTLEFQSTINPLRR"
     gene            200..802
                     /locus_tag="VFW59_01895"
     CDS             200..802
                     /locus_tag="VFW59_01895"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5362997.1"
                     /translation="MAVLVGKAAPDFNAVAVMGNNEINESFNLSKHISGKYAVVFFYP
                     LDFTFVCPSELIAFDHRLDEFKKRNVEVIGVSIDSQFSHFAWKNTPVNNGGIGQVRYP
                     LVADIKHEICRAYDVEAEGGVAYRGSFLIDRAGVVRHQVVNDLPLGRNIDEMLRMVDA
                     LQFTEEHGEVCPAGWSKGKAGMNASTEGVAKYLANHASEL"
     gene            939..2435
                     /locus_tag="VFW59_01900"
     CDS             939..2435
                     /locus_tag="VFW59_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX5362998.1"
                     /translation="MLIWFVTLYLLVSVGIGLYASTRVHNSRDFVVAGRNLPLPVVTA
                     TVFATWFGAETVLGISATFVQEGLGGVVADPFGASMCLIIAGLFFAPRLYRMNLLTIG
                     DYYRIRYNRAVELIMTLCIMLSYLGWVSAQVVALGLVFNLVSGGAVTQETGMIIGISI
                     VIAYTTFGGMWSVALLDFVQMTIIMAALLLIAVLIGGDAGGFANVVGHAQAAGKLQLL
                     PSGGMEVWVPFVGAALTMMLGSIPQQDVFQRMTSAKDEKTAVRGSVLGGVLYFAFAFI
                     PMFLAYAATLIDPGFFGNLIETDSQMVLPSLILNHTPLIAQVLFFGALLSAIMSTASA
                     TLLAPSVMFTENILKHFVFKHMSDRQMLRTIRIVLLSFGGLVLWFALNSQSSIFHMVE
                     DAYKVTLVGAFVPLAFGLFWKRANNQGAVLAIVFGLSAWLLMETLHPDTYWPPQLVGL
                     LLSVTGMLAGSLLPSVIGKPLPQEPEHAYLHHHAASQTGHVPHRHSDR"
     gene            2507..3193
                     /gene="phoB"
                     /locus_tag="VFW59_01905"
     CDS             2507..3193
                     /gene="phoB"
                     /locus_tag="VFW59_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX5362999.1"
                     /translation="MTAKILIVEDEPAIQELLAFNVMQAGFQALRAEDADSLWQQIRD
                     HRPDLILLDWMLPNTSGVILAKQLRADARTKDIPIIMLTARGEERDKVTGLESGADDY
                     ITKPFSPRELMARIRAVLRRRAPQASDDSVNAGGLELLPSAHRVSANGTNIELGPTEF
                     RLLHFFMTHAERVYSRTQLLDQVWGTQVFVEERTVDVHIRRLRAALEPLKMDGLIQTV
                     RGSGYRFSAN"
     gene            3242..>3602
                     /locus_tag="VFW59_01910"
     CDS             3242..>3602
                     /locus_tag="VFW59_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3329 domain-containing protein"
                     /protein_id="HEX5363000.1"
                     /translation="MQDFWFRVSQPVLYGSLFAAILWGIFSATVALSFLLLVLLVVMV
                     YRARQLYKLGLWLNNPLPETIPEADGLWDEVFSLLYKMVKQHKQTKQELAAELQHIEQ
                     ATAALPEGVAILNEANRI"
BASE COUNT          666 a         1089 c         1089 g          758 t
ORIGIN      
        1 cttttacttg gcaagacaag aaaggtaacc agttgccggt ctgcaccggc gattttgact
       61 ttggttgttc atgcttcgac ttcgctacgc tgagaaaaca accaaccatt ccaccttccc
      121 ggttgacagc aacgccgctg ccctttaaat tccataccct agaatttcag tcaaccatca
      181 accctttaag gagataaata tggcagttct cgtcggtaaa gccgcacccg atttcaacgc
      241 cgttgccgtc atgggcaaca acgagatcaa cgaaagcttc aacttgagca agcacatcag
      301 cggcaagtac gccgtggtgt tcttctatcc gctggacttc accttcgtgt gcccttcgga
      361 actgatcgct ttcgatcatc gtctggacga gttcaagaag cgcaacgtcg aagtgatcgg
      421 cgtgtccatc gactcgcagt tctcccactt cgcctggaag aacacccccg tcaacaacgg
      481 cggtatcggt caggtgcgtt accctctggt cgccgacatc aagcacgaga tctgccgtgc
      541 ttatgacgtc gaagcagaag gcggcgtggc ttatcgcggc tccttcctga tcgaccgcgc
      601 cggcgtagtg cgccatcagg tggtgaacga cctgccgctg ggccgcaaca tcgacgagat
      661 gctgcgcatg gtcgacgcgc tgcaattcac cgaagagcac ggcgaagtgt gcccggctgg
      721 ctggagcaaa ggcaaggctg gcatgaacgc ctccaccgaa ggcgtcgcga agtatctggc
      781 aaaccacgcc agcgaactgt aagcatcgcg ttcacgcgcc tccctcgcgg aggcggcaga
      841 actcaggaag ccacccacgg gtggcttctt tattggttga gattcggcct atcgctgcta
      901 gaatccgcga aatctttttt gctgagagac cgttcgtcat gctgatctgg ttcgtcaccc
      961 tgtatttgct ggtttcggtc ggcatcggcc tgtatgcctc cacgcgcgtt cacaattcca
     1021 gagacttcgt ggtggccggg cgcaacttgc cgctgcctgt cgtgacggcc accgtcttcg
     1081 ccacctggtt cggcgcggag accgtgctgg gcatatccgc gacctttgtg caggaaggcc
     1141 tgggcggtgt ggtggccgac ccgttcggcg ccagcatgtg cctgatcatc gccgggctgt
     1201 tttttgcacc gcgcctgtac cggatgaatc tgctcaccat cggcgactac taccggatcc
     1261 gctataaccg cgcggtcgaa ctgatcatga cgctgtgcat catgctgtcc tatctcggct
     1321 gggtgtctgc gcaggtggtg gcgctgggac tggtgttcaa cctggtcagc ggcggtgcgg
     1381 tgacgcagga gacgggcatg atcatcggca tctccatcgt gatcgcctac accaccttcg
     1441 gcggcatgtg gtcggtggcg ctgctggact tcgtgcagat gaccatcatc atggcggcgc
     1501 tgttgctgat cgcggtgctg atcggtggcg atgcgggcgg cttcgcgaac gtggtcggcc
     1561 atgcgcaggc ggcaggcaag ctgcaactgc tcccgagcgg cggcatggag gtgtgggtgc
     1621 ccttcgtcgg cgcggcgctg accatgatgc tgggctccat tccgcagcag gacgtgttcc
     1681 agcgcatgac ttccgctaaa gacgagaaga ctgcggtgcg cggctcggtg ctgggcggcg
     1741 tgctgtattt cgcattcgcg ttcattccga tgttcctggc ctatgcggcg accctgatcg
     1801 accccggctt cttcggcaac ctgatcgaaa cggattcgca gatggtgctg cccagcctga
     1861 ttttgaacca cacgccgctg atcgcgcagg tgctgttctt cggcgcgctg ctgtctgcca
     1921 tcatgagcac cgcctcggcg accctgctcg ccccgtcggt gatgttcacc gagaacatcc
     1981 tcaagcattt cgtcttcaaa cacatgagcg accgccagat gctgcgcacc atccgcatcg
     2041 tcctgctgtc cttcggcgga ctggtgctgt ggttcgcctt gaattcgcag tcgagcatct
     2101 tccacatggt cgaggatgcc tacaaggtca ccctggtcgg cgccttcgtg ccgctggcat
     2161 tcggcctgtt ctggaagcgc gccaataatc agggcgcggt actggctatc gtgttcggcc
     2221 tgagcgcctg gctgctgatg gagacgctgc acccggacac ctactggccg ccgcaactgg
     2281 tcggcctgct gctgagcgtg accggcatgc tggcgggttc gctgctgccg agcgtgatcg
     2341 gcaagccgct gccgcaggaa ccggaacacg cttatctgca tcatcatgca gccagccaga
     2401 ccgggcacgt tccccatcgc cactccgacc gctagggccg catgtcacac ccttgtcaca
     2461 aaatcgtcat acccttgcaa ccgagacatg agacagagga atggtaatga ctgcaaagat
     2521 tttgatcgtg gaggacgaac cggcgattca ggaactgctg gcgttcaatg tcatgcaggc
     2581 gggatttcag gcgctgcggg ccgaggatgc cgatagcctg tggcagcaga tccgcgatca
     2641 tcgccccgac ctgatcctgc tggactggat gctgcccaat accagcggtg tgattctggc
     2701 gaaacagctg cgcgccgatg cgcgcaccaa ggacatcccc atcatcatgc tgaccgcgcg
     2761 cggcgaggaa cgcgacaagg tgacggggct ggaatccggc gccgacgatt acatcaccaa
     2821 gcccttttca ccgcgcgaac tgatggcacg tatccgtgcg gtgttgcgcc gccgtgcgcc
     2881 tcaggcgtcg gatgattcgg tgaatgcggg cggactggag ctgctgccaa gcgcgcatcg
     2941 ggtgagcgcg aacggcacga acatcgagct cgggccgacc gaattcaggc tgctgcattt
     3001 tttcatgacc catgccgagc gcgtgtatag tcgcacgcaa ttgctcgacc aggtgtgggg
     3061 cacgcaggtg ttcgtcgagg agcgcacggt cgatgtccat attcgccgcc tgcgcgccgc
     3121 gctggaaccg ctgaaaatgg atggtctgat ccagacggta cgcggcagcg gttaccgttt
     3181 ttcggccaat taaaatcggc atccgggaca aagcccgggt cgtctagtca gggagacagt
     3241 tttgcaggac ttctggtttc gggtaagtca gcccgtgctg tacggcagcc tcttcgccgc
     3301 catcctctgg gggatattct ctgccacggt cgcgttgtcc tttctgctgc tggtgttgct
     3361 ggtggtcatg gtctatcgtg cgcggcaact ctacaagctg gggctgtggc tgaacaatcc
     3421 gctccccgaa accatcccgg aagcggatgg gctgtgggac gaagtctttt ccctgctcta
     3481 caagatggtc aagcagcaca aacagaccaa gcaggagctg gcggccgaac tgcagcacat
     3541 cgaacaggcg accgccgccc tgccggaggg ggtggcgatc ctgaacgagg ccaatcgcat
     3601 cg
//
LOCUS       DASUIV010000058         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1224262, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000058 DASUIV010000000
VERSION     DASUIV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224262"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..462)
                     /locus_tag="VFW59_01915"
     CDS             complement(232..462)
                     /locus_tag="VFW59_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HEX5363001.1"
                     /translation="MNPEHLQLLLTRVMPYGKYKGRVIADLPGNYLNWFAREGFPSGE
                     LGSLLALMHELDHNGLSHLLDPLRTSRKIQSR"
     gene            complement(545..1228)
                     /locus_tag="VFW59_01920"
     CDS             complement(545..1228)
                     /locus_tag="VFW59_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5363002.1"
                     /translation="MRIRLMLADDHELVRSGIIKLLKTSLNIEVVAESSCREELFDTL
                     RAVEVDVLLLGMVMPPIKGADMIARIKSDHPDLRVLVLSMFDDILIALRAMKAGASGY
                     ITTYGNPQELFGAVHKVAVTGRYLNPEMAEKLAFVSTVSGGRELELLLSDRERQIMSR
                     IVEGKCIKTIANELSISDKTVSTHKAHILAKLGVHNVPDLVRYVMKNESPIQYPHLAV
                     DHGNGALQY"
     gene            complement(1402..1875)
                     /locus_tag="VFW59_01925"
     CDS             complement(1402..1875)
                     /locus_tag="VFW59_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HEX5363003.1"
                     /translation="MSDSLNFSLSRDTYGRLVLATADGAVHVGVVAVRAFPIAAPDQG
                     ISLLDAEGHELAWIEHLSKLSEETRALLEQELASREFMPEIRRIKEVSSFATPSTWQV
                     ETDRGDTRFVLKGEEDIRRLSHTTLLIADSHGIQFLIGDLAALDRASRKLLDRFL"
     gene            complement(1872..>2629)
                     /locus_tag="VFW59_01930"
     CDS             complement(1872..>2629)
                     /locus_tag="VFW59_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5363004.1"
                     /translation="GFRYGNRSVIRGVDLDIAPGEMIGLVGHSGSGKSTLVNLICRFY
                     DVSEGAIRIDGVDIRSLPVAEYRRHIGLVLQEPFLFFGTIAENIAYGKPEATRAEIVA
                     AARAAHAHEFILRLPQGYDSLVGERGQGLSGGERQRISIARALLIDPRILILDEATSS
                     VDSTTEKEIQKALDNLVQGRTTIAIAHRLSTLRKADRLVVMDRGQVVEVGNHDALMAR
                     QGAYFQLYQAQARNVDREDEVPESGATPKEHAA"
BASE COUNT          559 a          807 c          717 g          546 t
ORIGIN      
        1 aaaccgcgta gtggtggccg gaaccacaaa gtcaaaaact tctttgcggc gtaggccgaa
       61 aagtttttgg ccttgcggtg aaggctaacc tgcttgcagg ttaagcagcc cgacggactg
      121 cggccgtagc cacaaagtaa agaatttctt tgcggcgtag atcgaggttg cggaagtagt
      181 taatgcgcca acaaaagacc tgcccggcct gatcgccggg caggtctttt tttatcgcga
      241 ctgaattttt ctgcttgttc gcaacggatc gagcagatgc gagagtccgt tgtgatccag
      301 ctcatgcatc agcgccagca ggctgccaag ctcgcccgac ggaaatccct cgcgagcgaa
      361 ccagttgaga tagtttcccg gcagatcggc gatcacacgc cccttgtact ttccataagg
      421 catgacgcgg gtaagcagta gctgcagatg ttcgggattc atcaaaaatc gatctatgaa
      481 gagggaaaag ctgtcatggc atgaaatatc cccccccaag ccagacgctt accccctgcc
      541 taacttaata ctgcagcgcc ccattgccgt gatcgacagc caagtgcgga tattgaatgg
      601 gcgactcatt cttcatcacg tacctcacca gatcagggac attatgcaca ccgagcttgg
      661 ccaatatatg tgccttgtgc gtgctaacgg tcttgtcact gatagacagc tcattggcaa
      721 tcgtcttgat gcacttccct tcgactatcc gcgacataat ctgccgctcc cggtcggaaa
      781 ggagcaactc gagttcgcgc ccaccggaga cggtcgagac aaaagccagt ttttcagcca
      841 tctcgggatt gaggtatctc ccggtcaccg caactttatg cacggctccg aacaactctt
      901 gcggattgcc gtacgtcgta atgtagccag aggcaccagc cttcatcgca cgcagtgcaa
      961 tcagaatatc gtcaaacata ctgagcacta acacccgcag atcgggatga tcgcttttga
     1021 ttcgagcaat catatcggcc cccttgatgg gcggcatgac catacccagc aacagcacat
     1081 caacctcgac cgcacgcaac gtgtcaaaca actcttcgcg acaagaggac tccgcaacca
     1141 cctctatatt taaacttgtt ttcaacaact taattatccc ggatcgaacc aactcatggt
     1201 cgtccgcaag catcagacgt attctcaagt cttcccctcg ttttgcattt tttattcgtt
     1261 ccgtccaaac acactttacg caaatctcat ttgcgcatcg cgccctttca tcggatcgat
     1321 tctatactgt ccccggtaaa atttacaatt gttcgctctg cggcattggc gccgccgagg
     1381 cccgccgctg agcgcggcga attacaggaa gcgatccagc agcttgcggc tggcgcgatc
     1441 cagtgcggcg aggtcgccga tcaggaactg gatgccatgg ctgtcggcga tcagcaaggt
     1501 ggtgtggctg agacgacgga tgtcttcttc gcccttaagc acgaagcggg tgtcgccccg
     1561 gtcggtctcg acctgccagg tactgggcgt cgcgaaactc gacacctcct tgatgcggcg
     1621 tatttcgggc atgaattcgc ggctcgccag ctcctgctcc agcagcgccc gcgtctcttc
     1681 cgacaatttg gacagatgtt cgatccaggc cagttcgtgc ccttccgcat cgagcagcga
     1741 gatgccctga tccggcgcgg cgatcgggaa ggcgcgcacc gcgaccacgc cgacatgaac
     1801 cgcgccgtcc gccgtggcca gaaccagccg tccataggta tcgcgcgaca acgagaaatt
     1861 cagtgagtca ctcatgctgc atgctccttt ggcgtagcgc cggattccgg gacttcgtct
     1921 tcacgatcca cattgcgcgc ctgcgcctga tagagctgga agtatgcgcc ctgccttgcc
     1981 atcagggcgt cgtgattgcc gacctcgacc acctgcccgc gatccatcac caccagccgg
     2041 tctgccttgc gcaaagtcga cagccggtgc gcgatcgcga tggtggtacg tccctgaacc
     2101 aggttgtcga gcgccttctg gatttccttc tcggtggtgg agtcgaccga ggaggtggcc
     2161 tcgtcgagga tcaggatgcg cgggtcgatc agcagcgcac gcgcgatcga gatgcgctgg
     2221 cgctcgccgc cggacaagcc ctgcccgcgc tcgccgacca gcgagtcata gccctgcggc
     2281 aggcgcagga tgaattcgtg ggcatgggcc gcgcgtgcgg cggcaacaat ctcggcgcga
     2341 gtcgcctccg gcttgccata ggcaatgttc tcggcgatgg tgccgaagaa caggaagggt
     2401 tcctgcagca ccagcccgat gtggcggcgg tattccgcca ccggcaaaga acggatgtcc
     2461 acgccatcga tgcggatcgc accctcgctc acgtcgtaaa aacggcagat cagattgacc
     2521 agcgtgctct tgcccgagcc gctgtgcccg accagcccga tcatctcgcc cggcgcgata
     2581 tccagatcga cgccgcgtat caccgagcgg ttgccgtagc ggaagccga
//
LOCUS       DASUIV010000059        11641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_202111, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000059 DASUIV010000000
VERSION     DASUIV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202111"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFW59_01935"
     CDS             complement(<1..952)
                     /locus_tag="VFW59_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5363005.1"
                     /translation="MKKQFTAILLTLGFSMHSLLAADYSSELDYFQELPVVLTASRLL
                     QPLSEAPNAMTVIDRKMIVASGFRTIPELFKQVPGMYVGYYKGNQPFVSYHGSPDQYA
                     RRMQVMIDGRSVYMPPMSMVDWTTLPITVEDIERIEVIRGPAAASHGANSTQGVISII
                     TRDADAVNGKQLSVTRGDKGVNDVAAHFGKRGEAFDYRMTLAYTADNGYDNLSAIPGS
                     VPRAALPAAGLLNNSFDNNQARLLNYRAGYHPNGVDSFDLQFGFNHDIKNVGWTDSPA
                     NPVHDLISNSGFLQLGWIRSLENAAELKVQYHHTRHDQHEA"
     gene            complement(1069..1145)
                     /locus_tag="VFW59_01940"
     tRNA            complement(1069..1145)
                     /locus_tag="VFW59_01940"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1109..1111),aa:Asp,seq:gtc)
     gene            complement(1158..3047)
                     /locus_tag="VFW59_01945"
     CDS             complement(1158..3047)
                     /locus_tag="VFW59_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX5363006.1"
                     /translation="MFDFVQEKKRLVQIVLAIIILPFAFWGVDSYNRSGNSAETVATV
                     NGAKITRQEFDEALGQQQERLRQMLGGTFDAAMFDKPEMKRAVLENLVEQRLLIERAK
                     ASGLAVTDQQVAQVIAGVEAFQQDGKFDKARYESALAGRKMTPLTFEARLREDLIGQQ
                     MHDVFAQNGFAANDVVDNVVRLNEQQRTISVATIGFESFLARAEISDAAVQDYYQQNQ
                     KAFHVPEQVRVEYVKFSADDLQAKEQVQEDVLRRYYDEHQQEFGTPEERRAAHILITA
                     AVTAPQADQDAAKARAEQLLQQVRQNPARFAELAKANSQDTGSAVNGGDLGFFGHGMM
                     VKPFEDAAYALKPGEISGVVKSDFGYHIIKLLEIKSAQVQPYSVVRDGISSKLRQQKA
                     TDRFAEQAERFSNTVYEQSDTLKPAAELVGAKIQQSDWLVKDAKAAGPWTDKMLQAVF
                     SDEAIKNKRNTAAIEVAPNTLVAARILEHKPATVQPLDEVQQGIRQKLMHQQALKLAE
                     EQGKATLVKLQQGDKSALGWGAVQSITRAQQGALDAGLVRQVFQANSDKFPQYVGAVT
                     GQGYVLVRIDGVKQGETPDEMKRMRYAQQLRQLTGEEMFQAYQADAKKHADIKLKLQE
                     AASAK"
     gene            complement(3121..3197)
                     /locus_tag="VFW59_01950"
     tRNA            complement(3121..3197)
                     /locus_tag="VFW59_01950"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3161..3163),aa:Asp,seq:gtc)
     gene            complement(3234..3309)
                     /locus_tag="VFW59_01955"
     tRNA            complement(3234..3309)
                     /locus_tag="VFW59_01955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3274..3276),aa:Val,seq:tac)
     gene            complement(3329..3601)
                     /locus_tag="VFW59_01960"
     CDS             complement(3329..3601)
                     /locus_tag="VFW59_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5363007.1"
                     /translation="MNKSDLIDAIATSADISKASAGRALDAAIESIKKALKKGDTVSL
                     VGFGTFKVGARAARTGRNPRTGEEIKIKAAKIPKFSAGKGLKDAVN"
     gene            complement(3745..6171)
                     /gene="lon"
                     /locus_tag="VFW59_01965"
     CDS             complement(3745..6171)
                     /gene="lon"
                     /locus_tag="VFW59_01965"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5363008.1"
                     /translation="MLEQDTPNTTQADLHPLLPLRDVVVFPHMVIPLFVGRAKSIKAL
                     EAAMDAGKGIVLVAQKSAAKDDPSKDDLYGIGSMANILQMLKLPDGTVKVLVEGTQRV
                     NVLHVYDTDSHFDAEVEIVEAEDGTSSESEAMRRALIAQFDQYVKLNKKIPPEILTSL
                     AGIDDAGRLSDTIAAHLPLKLEQKQEVLEIFGVRKRLEHLLGLLEAEIDILQVEKRIR
                     GRVKRQMEKSQREYYLNEQVKAIQKELGEGEEGGDLDELEKKIKAAQMPKEARAKAES
                     ELKKLRMMSPMSAEATVVRNYIDVLVGLPWKKKTKISAELKKAESVLEEDHYGLDKVK
                     ERILEYLAVQQRVDKLKAPILCLVGPPGVGKTSLGQSIARATNRKFVRMSLGGVRDES
                     EIRGHRRTYIGSMPGKILQNMSKVGVKNPLFLLDEVDKMGMDMRGDPSSALLEVLDPE
                     QNGTFVDHYVEVEYDLSDVMFVATANTLNIPEPLLDRMEIIHVSSYMEDEKVNIATRY
                     LVPKALKNNGLKPEDISISESALRDIVRYYVREAGVRGMEREISKVCRKVVKALALKA
                     SDKKVTINARNLEKYLGVRRYSYGIAEKVNQVGQVTGLAWTEVGGELLTIETAVLPGK
                     GKTTTTGKLGEVMQESIQAALSVVRSRAKRLGIDEEFYQKTDLHIHLPEGAIPKDGPS
                     AGIGMCTAMVSALTGIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAQRGGIKVVLIP
                     EENTKDLAEIPDNIKNKLDIHPVKWIDQVLELALERKPEPLVEPPEKIAVAELAVDGK
                     PVDPVVATKH"
     gene            complement(6274..7524)
                     /gene="clpX"
                     /locus_tag="VFW59_01970"
     CDS             complement(6274..7524)
                     /gene="clpX"
                     /locus_tag="VFW59_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5363009.1"
                     /translation="MSSDKKSGDKLLYCSFCGKSQHEVRKLIAGPSVFVCDECITLCN
                     DIMREENQTEVIGADKTDLPVPKDICDALGEYVIGQERAKRILSVAVYNHYKRLKSDS
                     RDGVELSKSNILLVGPTGSGKTLLAQTLARLLDVPFVMADATTLTEAGYVGEDVENII
                     QKLLQACDYNVEKAQRGIIYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTKA
                     SIPPQGGRKHPNTEFLQVDTTNILFICGGAFDGLEKVIRSRSKKASIGFGATISSNDE
                     RKTGETLREVEPEDLVKFGLIPEFVGRLPVVATLEELDEAALVRILTEPKNALTKQYQ
                     KLFDMEGVELEFREGVLAAVARRALARKAGARGLRSILEHALLDVMFDLPSMDNVEKV
                     VLDDAGVDGEIKPIIIYADNHKAA"
     gene            complement(7573..8181)
                     /gene="clpP"
                     /locus_tag="VFW59_01975"
     CDS             complement(7573..8181)
                     /gene="clpP"
                     /locus_tag="VFW59_01975"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX5363010.1"
                     /translation="MGRIEPQNIGLVPMVVETSGRGERAYDIYSRLLKERVVFLVGEV
                     NDYSANLIVAQMLFLESENPDKDIHFYINSPGGSVTAGMAIYDTMQFIKPHVSTLCIG
                     QAASMGAFLLTAGEKGKRFCLPNSRVMIHQPLGGFRGQASDIEIHAREILYLKQKLNQ
                     MLAHHTGQPVETIERDTDRDNFLSAEDAVKYGLVDQVLDKRP"
     gene            complement(8186..9484)
                     /gene="tig"
                     /locus_tag="VFW59_01980"
     CDS             complement(8186..9484)
                     /gene="tig"
                     /locus_tag="VFW59_01980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5363011.1"
                     /translation="MSSVETVSALERRLNASIPQQAIRTQVTARLKNIGRTAKIAGFR
                     PGKIPANVLEQHFGAQARQEALGDALQESFAEAAESNKLKVAGYPQFEIKTNDLNADQ
                     IEYSATFEVYPEVVLGDVAGETVERAVYEVGQADVENTIATLRKQRATFELADRAAQA
                     DDNVRIDFVGKLNGEVFQGGEAKDYPFVLGQGRMLPEFEAAITGMKAGETKSFNMTFP
                     ETYHGKDVAGKEVTFTITLNRVEAPKLPEVDAEFARSIGIEDGDVNKLESDILSNMKR
                     EVTRRLNARNKEAAMDALLKVAQFDVPKALVGWEAQNLMQQTVRDMESRGMQMKGMKL
                     PPELFADRAIKRVKLGLILADLVEKHGLSATPERVRALVDDYAQSYDQPEDVVRWYYA
                     DPARLQEVENLALEENVVAWVMGQVKTQDKAIAFNELMGN"
     gene            complement(9521..9605)
                     /locus_tag="VFW59_01985"
     tRNA            complement(9521..9605)
                     /locus_tag="VFW59_01985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9569..9571),aa:Leu,seq:tag)
     gene            complement(9681..>11641)
                     /locus_tag="VFW59_01990"
     CDS             complement(9681..>11641)
                     /locus_tag="VFW59_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5363012.1"
                     /translation="VIAQQVLGHFLDTLVDESLHSVMIALLLSAIFYYFVTRPLASLS
                     ERLRKLHPGNMTESTLQVPDGHDADEIGMVVSTMNRLIEELRYSEELLRADADQLRLH
                     AGIFKNSHDAITITDMNGNIEAVNPAFSTITGYSSDEVVGKNPRFLQSGRQTQAFYAA
                     MWNSLLSNGFWTGEVWNRRKDGSFYAAWLSISALRNAAGKVEHYIGVTSDNTDYKEAQ
                     ERIRKMAFYDQLTGLPNRSLLRDRVDRLFAQMRRGEKSFAIMFIDLDNFKRVNDSLGH
                     HMGDLLLQEVAHRLGHCVREVDTVSRQGGDEFVVLLPECDSDCAQAIAGRILDALSRP
                     HHLEGNELVATPSIGISMFPRDARDFETLSKHADTALYRVKGEGRAGFQFFMPEMNIA
                     AHKQLTVENQLRKAVESGGFTLHYQPQIDREQGKIIGMEALIRWDDPVLGSVPPDEFI
                     PITEENGLIVPIGTWVLHEACAQNMRWQAMGLPPVPVSVNVSAVQMRQPNFVQVVADA
                     LEQSGLKPELLELELTERVAMADVERSVQIMHQLNALGVGLFIDDFGTGYSSLAYLKR
                     FPLKKLKIDKSFVRDLETNADDRTIANSIILLAHGLNLSVVAEGVENVAQLDILNEQG
                     CDAVQGYYFSRALPADEFTEFFRQAQGG"
BASE COUNT         2500 a         3637 c         2832 g         2672 t
ORIGIN      
        1 aggcttcatg ctgatcatgc cgagtgtgat ggtattgcac cttcagctcc gcagcattct
       61 ccagtgacct tatccagccc agttgcagaa aaccggaatt ggaaatcagg tcgtgaaccg
      121 ggttcgctgg gctgtcagtc cagccgacat ttttaatatc atggttaaag ccaaactgaa
      181 gatcgaagct atcgacaccg ttgggatgat aaccggcgcg ataattcagc agtcgcgcct
      241 gattgttgtc aaaactgtta ttgagtagcc ccgctgcggg aagtgctgca cgaggaacgc
      301 tgccggggat tgcgctcaaa ttgtcgtaac cgttgtcggc cgtgtaagcg agcgtcatac
      361 gataatcgaa cgcctctcct cgcttgccaa aatgggcagc gacatcgttg acgcccttat
      421 cgccacgcgt gaccgaaagt tgcttaccat tcacagcatc agcatcccgc gtaatgatgc
      481 taatcacccc ctgcgtcgaa ttcgcgccat gcgatgctgc cgccgggccg cgtatcacct
      541 cgatgcgttc aatatcctca acggtgattg gcaaggtagt ccaatccacc atgctcatcg
      601 gcggcatata cacgctgcga ccatcgatca tcacctgcat gcgtctagca tactgatccg
      661 gcgaaccatg gtaggagaca aaaggctgat tacccttgta gtagccgaca tacatacccg
      721 gaacctgctt gaacaattca ggaatcgttc gaaatccgga tgccacgatc atcttgcgat
      781 cgatcaccgt catcgcattc ggcgcttctg acagcggttg caaaagacgc gaggctgtca
      841 gcacaacggg cagctcctga aaatagtcca gctcacttga atagtcggca gccaggagac
      901 tatgcattga aaaacctagc gttagcagga tcgccgtaaa ttgcttcttc atgttcatac
      961 ccaatatgaa tagacgggga atattaacat caattaaagc ggcgaacagc cctgcagcct
     1021 cggcgaacga caggcgataa aaaaggcgaa ctttcgttcg ccttgtgttg gcggagtgga
     1081 cgggactcga acccgcgacc cccggcgtga caggccggta ttctaaccaa ctgaactacc
     1141 actccataaa ctcttcccta cttggcactc gcagcttctt gcagcttcag cttgatatcc
     1201 gcatgctttt tggcatcagc ctgataggcc tggaacatct cttcacccgt aagctgacgc
     1261 aattgctggg cataacgcat gcgcttcatc tcgtcgggag tctccccctg cttgacacca
     1321 tcgatgcgca ccagcacgta gccttgacca gtcaccgcac cgacatactg cgggaactta
     1381 tcgctattgg cctgaaatac ctgacgcacc aaaccggcat ccagcgcgcc ctgctgcgcg
     1441 cgggtaatgc tctgaaccgc accccagcca agagcagact tgtcgccctg ctgcagtttc
     1501 accagcgtgg ctttgccctg ctcttcagca agtttcagcg cctgctggtg catcagcttc
     1561 tggcgtatgc cctgctgcac ctcatccaaa ggctgcaccg tcgcgggttt atgttccaga
     1621 atccgcgccg cgactaaagt attgggtgcg acttcgatgg ctgcggtgtt gcgtttattc
     1681 tttatcgcct catcgctgaa taccgcctgc aacatcttgt cggtccacgg cccggcagct
     1741 ttcgcatcct tgaccagcca atcgctctgc tgaatctttg caccgaccaa ctcggcagcc
     1801 ggtttcaagg tatcgctctg ctcataaacc gtattgctga accgctctgc ctgctcggca
     1861 aacctatccg ttgctttttg ctgacgcagc ttgctgctga tgccatcacg caccacgctg
     1921 tagggctgta cctgggcgga cttgatctca agcaacttga tgatgtgata gccgaaatcg
     1981 gatttcacca cgccgctgat ctcgccgggc ttgagcgcgt aggcagcgtc ctcaaacggt
     2041 ttgaccatca tgccatgacc gaagaaaccg aggtcgccgc cattcacggc cgaacccgta
     2101 tcctgcgagt tggccttggc caactccgca aaacgggcag gattctgcct gacctgctgc
     2161 aataattgct cggcacgagc cttggccgca tcctgatcgg cctgaggtgc agtgacggca
     2221 gccgtgatca gaatgtgtgc ggcgcggcgc tcctcgggcg tgccgaattc ctgctgatgc
     2281 tcatcgtaat aacggcgcaa cacatcctct tgcacctgct ctttcgcctg caaatcgtcg
     2341 gccgaaaatt tcacatactc gacgcgaacc tgctctggga catgaaacgc cttctgattc
     2401 tgctgatagt agtcctgcac tgcggcatcg ctaatttcag cgcgtgcgag gaaagactcg
     2461 aagccgattg ttgccacgct aatagtgcgc tgctgctcgt tcagacgaac gacattgtcc
     2521 accacatcat tggcagcaaa accgttctgc gcaaagacat catgcatctg ctgtccgatc
     2581 aggtcttcgc gcaggcgggc ctcaaaggtg agcggcgtca tcttgcggcc tgccagcgcc
     2641 gattcataac gagctttatc gaattttccg tcctgctgga aggcttcaac ccccgcaatc
     2701 acctgcgcca cctgctggtc agtcaccgca agccccgaag cctttgcgcg ttcgatcagc
     2761 agacgttgtt caacaagatt ttccaacacg gcccgcttca tctccggctt gtcgaacatc
     2821 gccgcatcaa aggttccgcc caacatctgg cgcaaacgct cctgctgctg ccccaacgcc
     2881 tcatcgaatt cctgacgagt aatttttgca ccattgaccg tagcgaccgt ctccgccgaa
     2941 ttgccggagc ggttatatga atcgaccccc cagaatgcaa acggcagaat gatgatggcc
     3001 agcactattt gtaccagtcg ttttttctct tggacaaaat caaacatagt gataggactc
     3061 gattattttt tctggcaaac gccagatatg aaaaaaggcg aactttcgtt cgccttaatt
     3121 tggcggagtg gacgggactc gaacccgcga cccccggcgt gacaggccgg tattctaacc
     3181 aactgaacta ccactccata aactattaca actcaacttc tactacaaaa tactggtggg
     3241 tgctgacggg gtcgaaccgc cgacattcgc cttgtaaggg cgacgctcta ccaactgagc
     3301 taagcacccc ttgaccaact aaagaacgct agtttacagc atctttcaaa cctttgccag
     3361 cagaaaattt aggaatttta gctgctttga ttttaatttc ttcaccggtg cgcggattgc
     3421 ggcctgtacg tgcggcgcgt gcaccgactt tgaatgtacc gaaacctacc aggctcacgg
     3481 tgtcgccttt tttcagcgcc ttcttgatgg attcaatagc cgcatcgagc gcgcgacctg
     3541 cagacgcttt ggaaatatcg gcagatgttg caattgcatc gatcagatca gatttgttca
     3601 cgttgctccc ctttgctcat tatttttaaa aacatgacac cacgctgcag cgcgctttat
     3661 atcaaggctg cgcgaggttg gtcaagcgat tactggaaat atttttttga tttcccctct
     3721 cgattcgaga ggggattttc cgctctagtg cttggtcgcg acaaccggat cgaccggctt
     3781 gccatccacc gccaattcag caactgcgat tttttcaggc ggctcaacca acggctccgg
     3841 cttacgctcc agcgccaatt ccagcacctg atctatccat ttcacaggat ggatgtccag
     3901 cttgtttttt atgttgtccg gaatctcggc caagtccttg gtgttctctt cggggatcag
     3961 cacgaccttg atgccgccgc gctgtgctgc caacagcttc tccttcaaac cgccgatcgg
     4021 caatacctcg ccacgcaacg taatctcacc cgtcatggcg acatcggctc gcaccggaat
     4081 gcccgtcaac gcagagacca ttgcagtgca catgccgata cccgcgcttg gaccatcttt
     4141 agggatagcc ccttcaggca agtgaatatg caaatccgtc ttctgataga actcttcatc
     4201 tatccccaaa cgcttggcac ggctacgcac cacactcaag gccgcctgaa tcgactcctg
     4261 cattacctcg cccagcttgc ctgtcgtcgt ggtctttccc tttccaggca gaacagcggt
     4321 ctcgatagtc aacagctcac cgccgacttc ggtccaggcc aaaccggtta cctgaccaac
     4381 ctgattgacc ttctctgcta ttccataact gtaacgacgc acccccagat atttttcgag
     4441 attacgcgca ttaatcgtga ccttcttgtc actcgccttc agcgccagcg ccttcaccac
     4501 cttacgacag accttggaaa tctcgcgctc cataccgcgc acgcccgctt ctcgcacgta
     4561 ataacgcacg atgtcgcgca atgcactttc cgagatgctg atgtcttccg gcttgagacc
     4621 attattcttg agcgccttgg gcaccagata acgagtcgcg atattgacct tctcatcttc
     4681 catataactg gaaacatgga tgatctccat acgatcgagc aacggctcag ggatatttaa
     4741 agtattcgcc gtcgccacga acatcacgtc ggaaagatcg tattcgacct cgacgtaatg
     4801 atcgacaaaa gtgccgttct gctccggatc gagcacttcc agcagcgcag aggatggatc
     4861 accccgcatg tccatgccca tcttgtccac ttcgtcgagc aggaacagcg gattcttcac
     4921 gccgaccttg ctcatgttct gcagaatttt gcccggcatg gaaccgatgt aagtgcggcg
     4981 gtgaccgcga atctcggatt cgtcgcgcac cccgcccagc gacatgcgca cgaacttgcg
     5041 gttcgtagca cgcgcgatgg actgtcccag cgaggtctta cccacgcctg gaggccccac
     5101 cagacacaag atgggcgcct tcagcttatc cactcgctgc tgcacagcca gatactcaag
     5161 gatgcgttcc ttgaccttat ccagaccgta atgatcttcc tccagtaccg actcggcctt
     5221 cttcaactcg gcgctgatct tggtcttctt cttccaaggc aggccgacca gcacatcgat
     5281 atagttccgc accacggtgg cttcagccga catcggcgac atcatgcgca gcttcttgag
     5341 ctccgactca gccttggctc gcgcctcttt aggcatctgt gctgccttga tctttttctc
     5401 aagctcgtcg agatccccgc cctcttcgcc ttcgcccagt tctttctgaa tcgccttgac
     5461 ctgctcgttc aggtaatact cgcgctgact cttctccatc tggcgcttta cgcgaccacg
     5521 gatacgtttt tcgacctgca gaatgtcgat ctcggcctcc agcaagccca gcagatgctc
     5581 cagacgcttg cgcacgccga agatctccag cacttcctgc ttttgctcca gcttgagcgg
     5641 caaatgagcg gcaattgtat cggacagacg acccgcatca tcgatgccgg ccagcgaggt
     5701 cagtatctcg ggcgggatct tcttgttgag cttgacgtac tggtcgaact gggcgatcaa
     5761 cgcgcggcgc atcgcctcag actcgctgct tgtgccatct tcggcctcaa cgatctccac
     5821 ctcggcatcg aaatggctgt ccgtgtcata cacgtgcaac acattcacgc gctgcgtgcc
     5881 ttccaccagc accttcacgg tgccatccgg caacttgagc atttgcagga tgttcgccat
     5941 gctgccgatg ccgtacagat cgtccttgct cggatcatcc ttggccgccg atttttgcgc
     6001 gaccagcacg atgcccttgc ctgcatccat ggcagcctca agcgccttga tggatttagc
     6061 tcgacccaca aacaacggga tgaccatatg cggaaacacc accacgtcgc gcaacggcaa
     6121 cagtggatgc aaatccgctt gagtagtatt gggggtatct tgttctaaca tggcagcaac
     6181 ctatacgtta actgaggggc tagatgggga cgaccgtgaa aaattcaagc cgccccaccg
     6241 ggacaagata acaaagagcg agggctgcct gaatcaggcg gccttgtgat tatccgcata
     6301 gatgatgatc ggtttgattt cgccatccac accggcgtcg tccagcacca ccttttcgac
     6361 attatccatg gacggcagat cgaacatcac atcgagcaga gcatgctcca gaatagagcg
     6421 cagaccacgg gcaccagcct tgcgggccaa ggcacgacgg gccaccgcag ccagcacacc
     6481 ctcgcggaat tccagctcca caccttccat atcaaacagc ttttgatact gcttggtcaa
     6541 ggcgtttttg ggttcggtca ggatgcgcac caaagcggcc tcatccagct cttccagcgt
     6601 cgccacaacc ggcaagcgcc cgacaaactc ggggatcagg ccaaatttaa ccaagtcctc
     6661 cggctccacc tcgcgcagag tctcacccgt cttgcgctcg tcgttgctgc tgatggtcgc
     6721 accgaaaccg atgctggcct tcttggagcg gctacgaatc accttctcca atccatcgaa
     6781 ggccccgccg cagatgaaca gaatattggt ggtatccacc tgcaaaaact cggtgttcgg
     6841 atgcttgcga ccaccctgcg gcggaatcga tgccttggta ccttcgatca gcttgagcag
     6901 cgcctgttgc acgccctcgc cggacacatc tcgcgtaatc gacgggttat cagacttgcg
     6961 tgaaatcttg tcgatctcgt cgatgtagat gataccgcgc tgcgccttct ccacgttgta
     7021 atcgcaggcc tgcaacagct tctgaatgat gttctcgacg tcctcgccga cataaccagc
     7081 ctcggtcaac gtagtcgcat ctgccatcac gaacggcaca tccagcagac gcgccagcgt
     7141 ttgtgccagc aatgtcttgc ccgaacctgt cggtcctacc agcaggatat tgctcttgga
     7201 aagctccacg ccatccctgc tgtcgctctt gagacgcttg tagtggttat acaccgccac
     7261 cgacaggatg cgtttggccc gctcctgtcc gatcacgtac tcgcccagtg catcgcaaat
     7321 atccttcggc accggcaaat ctgtcttgtc tgcgccgatg acctcggtct gattctcctc
     7381 gcgcatgatg tcattacaca gcgtgatgca ctcatcgcac acgaacaccg acgggcccgc
     7441 aatcaacttg cgcacctcat gctgactctt gccacagaac gaacagtaga gcagcttatc
     7501 gcctgacttc ttatcgctgg acattcttcc ccataccttc catcaaatta attgctatgc
     7561 ctgccgcttc gcttacgggc gcttatccag cacctgatcc accaagccgt atttaaccgc
     7621 atcttcagcg ctcaggaagt tatcacgatc ggtatcgcgt tctatcgttt ccactggctg
     7681 accagtgtga tgagccaaca tctggttgag cttctgcttc aaatacagga tctcgcgcgc
     7741 atgaatctcg atatccgaag cctggccacg gaaaccgccc agtggctgat gaatcatcac
     7801 gcgggagttc ggcagacaga accgcttacc cttctcgccc gccgtcaaca ggaacgcgcc
     7861 catgctggcc gcctggccga tacacagcgt actgacgtgc ggcttgatga attgcatggt
     7921 gtcgtagatt gccatgcctg cagtcaccga gccgcctggg gagttaatat agaaatggat
     7981 gtccttgtcc gggttctccg attcaaggaa cagcatctgc gccacaatca ggtttgcgct
     8041 gtagtcgttc acctcgccca ccagaaacac cacgcgctcc ttgagcaggc gggagtaaat
     8101 gtcatacgcc cgctcaccac gcccgctggt ctcgacgacc atcgggacca atccgatatt
     8161 ttgtggctct atgcgaccca tcgcgttagt tccccatcaa ttcgttgaag gcaatcgcct
     8221 tgtcttgcgt cttcacctgc cccatcaccc aggctacgac attctcttcc agtgccagat
     8281 tctcgacttc ctgcagacgt gccggatctg cgtagtacca gcgtaccacg tcttcgggct
     8341 ggtcgtagct ttgcgcgtag tcatccacca gcgcgcgcac acgctccggc gtcgcactca
     8401 aaccgtgctt ctcgaccaag tcagccagaa tcagccccag cttgacgcgc ttgatcgcac
     8461 gatcggcgaa caactccggc ggcaacttca tgcccttcat ctgcatgccg cgtgactcca
     8521 tgtcacgaac ggtctgctgc atcagattct gggcttccca accgaccagc gccttgggca
     8581 cgtcgaactg cgcaactttc agcagcgcat ccatcgccgc ttccttgttg cgcgcattca
     8641 gacgacgtgt aacttcgcgc ttcatgttgc tgaggatatc gctctccagc ttgttcacgt
     8701 cgccatcctc gataccgatg gaacgcgcga attccgcgtc cacttccggc agcttgggcg
     8761 cctcgacgcg attcagcgtg atggtgaacg tcacctcttt accggccaca tccttgccat
     8821 gataggtttc cgggaaggtc atattgaaag acttggtctc gccagccttc atgccggtga
     8881 tcgcagcctc gaactcgggc aacatgcgcc cctgccccag tacgaacggg taatccttgg
     8941 cctcaccgcc ctggaacact tcaccattca gcttgcccac aaaatcgata cgcacattgt
     9001 catccgcctg tgctgcgcga tcggccaatt caaacgtggc acgctgcttg cgcaaggttg
     9061 caatggtgtt ctccacatcg gcctgaccca cttcgtaaac cgcacgctcc acggtctcgc
     9121 cagcgacatc gcccagcacc acttccggat agacttcaaa agtcgcactg tactcgatct
     9181 gatcggcatt caagtcgttg gttttgatct cgaattgcgg atagcccgcc actttcagct
     9241 tgttgctctc ggcggcttcg gcgaacgact cctgcaatgc atcgcccagg gcttcctgac
     9301 gcgcctgagc accgaagtgc tgctccagca cattggctgg aatcttgcca ggacgaaaac
     9361 cggcgatctt tgcagtacgc ccaatatttt tcagacgcgc agtgacctgg gtgcgaatgg
     9421 cttgttgcgg aatggatgca ttaagacgac gctcgagtgc gctcacggtt tctacgctgg
     9481 acatactgta tttccttgag attaaagttg aatttgttgc tggtgcgaaa gaagagactc
     9541 gaactcttac gccttgcggc gctggaacct aaatccagtg cgtctaccaa ttccgccact
     9601 ttcgcgctga catgcttcca gcttgggggc gaattatagc atcactccac cagctttcac
     9661 gcggtagcga ttccaaagat tcacccgccc tgcgcttggc ggaaaaattc cgtgaactcg
     9721 tccgccggca gcgcccggga aaagtaatac ccctgcaccg cgtcgcaacc ttgctcgttc
     9781 aggatatcga gctgtgcgac attttccacc ccttcggcca caaccgacag gttcaggcca
     9841 tgcgccaaca ggatgatcga gttggcaatg gtgcggtcat cggcattggt ctcaagatcg
     9901 cgcacgaacg acttgtcgat cttgagtttc ttcaacggaa agcgtttcag gtaggccagg
     9961 ctggagtaac ccgtgccgaa atcatcgata aacaacccga cgcccaacgc gtttagctga
    10021 tgcatgatct gcacagaacg ctccacgtcg gccatggcga cccgttcggt cagttccagc
    10081 tcaagcaact cgggcttcaa tccgctttgc tccagcgcat cggcgacgac ctgcacaaaa
    10141 ttcggctggc gcatctgcac cgcagaaacg ttcactgaaa ccggcacggg aggcagcccc
    10201 attgcctgcc agcgcatgtt ctgagcacag gcctcatgca atacccaggt cccgatcggc
    10261 acgatcagcc cgttttcctc ggtgatcggg atgaattcgt ccggtggcac cgaacctaac
    10321 accgggtcgt cccatcgaat caacgcctcc ataccgatga tcttgccctg ttcccggtcg
    10381 atctgcggct ggtagtgcag cgtaaatccg ccgctctcca ccgccttgcg caactggttt
    10441 tcaaccgtca gttgcttgtg tgccgctatg ttcatttccg gcataaagaa ctggaatccg
    10501 gcccgcccct ccccttttac ccggtagagc gcggtatccg catgcttgct caatgtttcg
    10561 aaatcccgcg catcccgagg gaacatgctg attccaatgc tcggtgtggc gaccagttca
    10621 ttcccctcca gatgatgggg gcgcgaaagc gcatcgagta tccgccccgc gatcgcctgc
    10681 gcacagtcgc tatcgcactc cggcaacaac accacgaact cgtcgccacc ttgccgcgat
    10741 acggtatcga cctcgcgcac gcagtgcccc aggcgatgcg ccacttcctg aagcagcaga
    10801 tcgcccatgt gatggccgag cgaatcattg acccgcttga agttatcgag atcgatgaac
    10861 atgatggcaa aggatttctc gccgcgacgc atctgagcga acagccgatc cacgcgatcg
    10921 cgcagcaggc tgcgattcgg caaccccgtc aactggtcgt agaacgccat tttacgaatg
    10981 cgttcctggg cctctttgta atccgtgttg tcgctggtga caccgatgta gtgttcgacc
    11041 ttccccgccg cattgcgcag ggcgctgatc gacaaccagg cggcatagaa gcttccgtcc
    11101 ttgcgacgat tccacacctc gccagtccag aatccgtttg acagcaaact gttccacatc
    11161 gcggcgtaaa acgcctgagt ttgccgaccg gattgcaaaa agcgcggatt cttgccgacc
    11221 acttcatcgg aggagtatcc ggtgatcgtg ctgaatgcag ggttgaccgc ctcgatattg
    11281 ccattcatat cggtgatggt gatggcatca tgactattct tgaaaattcc ggcatgcaat
    11341 cgcagctggt cggcatcggc gcgcaacaat tcctcggaat agcgcaattc ctctattaac
    11401 cggttcatcg tgctcacgac catgccgatc tcgtccgcat cgtgcccgtc aggcacctgc
    11461 aaggtcgatt ccgtcatatt gcccggatgc aatttgcgca gccgttcgga caaactggcc
    11521 agtggccgcg tcacaaaata atagaagatg gctgacagca gcagcgcgat catcacgctg
    11581 tgcaaggact cgtccaccag cgtatccaga aagtgaccca atacctgctg cgcaatcacc
    11641 t
//
LOCUS       DASUIV010000060        29598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1317326, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000060 DASUIV010000000
VERSION     DASUIV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 29598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29598
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317326"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFW59_01995"
     CDS             <1..641
                     /locus_tag="VFW59_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HEX5363013.1"
                     /translation="LGMCPLLAVSTSVVNGASLGFATAVVMALSNASISPIRNFVPNE
                     IRIPVFILIIAVLVTVVQYLMNAYMYGLYVLLGIFIPLIVTNCIVLARVEAFASKNPV
                     LPAALDGLAMGLGLTAVLGTLGGIRELFGHGTLFSGIELVFGEAAKSWVITVVPDYHG
                     FLLAVLPPGAFITLGLLIAMRNWLTLRAAQKTNGAAASIAPIEGGCSTTHTA"
     gene            638..1270
                     /gene="nth"
                     /locus_tag="VFW59_02000"
     CDS             638..1270
                     /gene="nth"
                     /locus_tag="VFW59_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5363014.1"
                     /translation="MNAAKRREIFLRLQAANPHPVTELEYHTPFELLIAVMLSAQATD
                     ISVNAATRQLYPVANTPQAILDLGEAGLRGYIQRIGLYQTKAKHVIQTCRLLLECHAG
                     EVPQAREELEALPGVGRKTANVILNTAFGQPTIAVDTHIFRLANRIGLAPGKTVLAVE
                     QKLLKVIPDEFKHDAHHWLILHGRYICQARKPKCGQCIIHDLCEYKDKQD"
     gene            1332..1754
                     /locus_tag="VFW59_02005"
     CDS             1332..1754
                     /locus_tag="VFW59_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEX5363015.1"
                     /translation="MLFNPSRDEARNFLIESWRKRRANELLTPLEDLAAQLIAKHPEH
                     HAVFEDGQLDRDYLPEHGATNPFLHLMMHLTIEEQISIDQPPGIRGHFARLTRKFESE
                     HEAQHAMMECLTEMIWQAQRDRTQPDAAIYLDCLVRQQ"
     gene            1834..4902
                     /locus_tag="VFW59_02010"
     CDS             1834..4902
                     /locus_tag="VFW59_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5363016.1"
                     /translation="MLQNFLPAALIILALGGVFFEFESAEDLRTLIDHEQDHVHAGVE
                     TANRTLLTLKRDTQNLAHSTTLGEAILQPSRTSRLEQDMLAFISSKVVYYKARWIDEN
                     GQERARVDIQNGKAVIVPSSRTENKATRDYFAQTIKLSDGATYLSPLDLEIEHGAIEQ
                     PLRPTLRAATPLFDAAGRKHGLAVVSYSAQDLFRRIQEISATEHSSWMLLNEQGYWLR
                     SGDPAQEFGFMLPHRASMAEQHPEIWDRIKAAPVGNFIDADGGLWLFETIYPHRVVND
                     QGTQDDGEASYWKMVKHFDAAELGGMQQYRRTQTLILVSFVMALALAISFRLSRSQID
                     KEDRDIDLHHLLGELNQQQFALDQHAIVAVADLNGTINYVNDKFCAISGYRREELIGQ
                     NHRIVKSGQHSNAFYREMSEAIKQGKVWNGETCNRAKDGSLYWVKNTFVPLLSARSYI
                     AISTDITAQKAVEEKLRIASIAFETHEAIVVTDAQARIVSVNRAFEQLTGYSAEEALG
                     QNPRILQSGRHDKEFYTEMWATLLVKGVWSGEMWDQRKDGSAYPKWLTITSVRNDKNE
                     ITNYVANFMDISERKRAEDEIQRLAFYDTLTELPNRRLLMDRLIQSLLNSERSGAYGA
                     LLFMDMDNFKVLNDTQGHDVGDMLLIEVANRLRGCVRESDTVARLGGDEFVVILQGLG
                     NSEILAANQAEDLAEKIVESLSKPYFLAAHEHHSSVSIGVGLFLGRGTKVDELLKRAD
                     TAMYQAKSSGRNAVRFFESAMQNAVESRAILEKQLRQALSRNELQLYYQIQVNQERRV
                     VGAEALLRWINEEQGFISPAQFIPLAEESGLILPIGLWVLDTACQQLKQWQDSPHARH
                     LQLAVNVSARQFRQPKFVEQVKNLLDKYAIDPSRLKLELTESVVLADVDETVQKMLAL
                     KQFGVQFSMDDFGTGYSSLSYLKQLPLDQIKIDQSFVRDIVVDKSDAVIVKTIIDMSI
                     NFNLEVIAEGVETEEQHEILRQNGCHAFQGYLFSKPIPIGEFEQLISRNA"
     gene            complement(4964..5422)
                     /locus_tag="VFW59_02015"
     CDS             complement(4964..5422)
                     /locus_tag="VFW59_02015"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEX5363017.1"
                     /translation="MNVLTRRLPGHIMARSEYSLVSLTFQGGIMVEQKAKAAVVKKTA
                     AVTKSKSAAKEAPVAKKVAAKKVDSVEKKIATSAVKKDAAAKKTAAPAVEKKAPAKKS
                     AASKAKPSPEERYRMVEMAAYFIAERHGFQGNSTEHWAAAEREIAEMLGS"
     gene            5491..6567
                     /locus_tag="VFW59_02020"
     CDS             5491..6567
                     /locus_tag="VFW59_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HEX5363018.1"
                     /translation="MKHLKSEYPIALALAVLGLGFALEHSAAEQGGAILWGLLLVILA
                     AVIGVAFRIAHHAEVLAIRFGEPYGTLILTLAAVSVEVVILVVLLSGEPNPTLARDTV
                     FAAVMFDINGILGLAAIVGGLKHGSSKYNVDSANSFIAMLLVAIGIGMFIPDFVPEEK
                     WQVYSVFSIGIMAIMYLAFLRLQTVEHRKFFEYSAETDEEAHDETHSPNALHVGVMLA
                     AIVLVGLLSEVLSVLLDAGLAGSSLPKAIPAVLVALISASPEILTALKAAQQDRMQTT
                     VNIALGASLATVLLTLPVIEAIALLSGERIEMALTPVQAGMLLLTFLTVMNNLHDGET
                     NAIEGISHFALFAAFIALVTIGLI"
     gene            6567..7034
                     /locus_tag="VFW59_02025"
     CDS             6567..7034
                     /locus_tag="VFW59_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363019.1"
                     /translation="MFALETWELLSYVVTVIGLPLAIAVFVFEQRKERDNEEEEVYQL
                     LSDNYQEFLKIALTNPDLRLFATEESSVRTPEQSERMFIIFSMLISLFERAYLLLYED
                     GMNEKQLRRWRSWEDYMGEWCDRTDFRAALPALLRGEDPEFAAYIQRLASRHS"
     gene            complement(7025..8137)
                     /locus_tag="VFW59_02030"
     CDS             complement(7025..8137)
                     /locus_tag="VFW59_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5363020.1"
                     /translation="MILILDANAGEQSPEYRQLLAHLATLQGIQTRVHVERGVEQNLT
                     EIYLIGDTKALDIGEMQSLPCVERVVRVSEEYRILGRHKDEQRPTHFDYNGVRFGQDT
                     LHVFAGLCAVDNREHVEIMMKAVRDAGQVCTRMGAYKPRTSPYAFQGHGKNCLPYVFE
                     LAGKYGIKVIAMEITHESHLDEIRAALEQTGNPTGVMLQIGTRNTQNFELLKIVGRQT
                     EMPIMLKRGFGITLDESLNAAEYLASEGNRKVIFGLRGMKTNMGDPHRNFVDFAHVPV
                     VKRLTRMPVCIDPSHSVGSRAASPDGILDMMHVTAQGVLAGANMVLVDFHPAPPKALV
                     DGPQAMLLKELPYFLEDVQIAREAYLKRVALRQRQE"
     gene            complement(8150..8728)
                     /gene="slmA"
                     /locus_tag="VFW59_02035"
     CDS             complement(8150..8728)
                     /gene="slmA"
                     /locus_tag="VFW59_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEX5363021.1"
                     /translation="MASKPGERKLQILQTVAHMLEQPKGEKVTTAALAARLELSEAAL
                     YRHFASKAQMYEGLIEFIEQTVFGLINRITQDEKSGLQQVESIVSLLLGFAQKNRGMT
                     RVLIGDALVNEDERLQLRINQLLDRVEATLKQSLRIAATQGEMGEAVDVGAHANLLVC
                     YVIGRWQQFAKSGFTRDPLAQWQAQSAILLQK"
     gene            complement(8736..9380)
                     /locus_tag="VFW59_02040"
     CDS             complement(8736..9380)
                     /locus_tag="VFW59_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX5363022.1"
                     /translation="MGGIVWIFDLDNTLHDASPHIFPHINRSMTAYLQQHLQLDEDEA
                     NALRMDYWQRYGATLSGMMLHHGTDPDHFLWHTHQFPELPQMVLREPRLRHVLKNLPG
                     KKMVFSNAPQHYAHAVLRLLRVDDLFDDVMAVEQTRYRPKPDSFGFLRLLRRHRIRAA
                     QCVMVEDSLENLQAAKRLGMGTVWVNAGSRGAPCVDVKIRDVMQLPRALHRLNR"
     gene            complement(9380..10264)
                     /gene="argB"
                     /locus_tag="VFW59_02045"
     CDS             complement(9380..10264)
                     /gene="argB"
                     /locus_tag="VFW59_02045"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5363023.1"
                     /translation="MSLTPSAAEKKARTLSEAMPYIQRFFDKTIVVKYGGNAMTDPML
                     QDSFARDVVLLKLVGMNPVVVHGGGPQINDLLNRVGKQGTFIQGMRVTDEETMDVVEM
                     VLGKVNKDIVNLINRHGGKAVGLTGQDGAFIRAEKMMLESKDEPGKMLDIGLVGEITK
                     IDPAIITFLDSGDFIPVIAPIGVAADGETLNINADVVAGKLAEVLKAEKLVLLTNTPG
                     VLDKAGNLLSGLTPKNIDDLVADGTLSGGMLPKISSALDAARSGVKSVHIIDGRVEHA
                     LLLEILTDEGVGTLIKSK"
     gene            complement(10428..10910)
                     /locus_tag="VFW59_02050"
     CDS             complement(10428..10910)
                     /locus_tag="VFW59_02050"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX5363024.1"
                     /translation="MISLYRFGGVLAGLLLSASLYAADVQVEGAWSRATAPGQDTAMV
                     DMSLTSKQDATLVGLSSPACKGVELHSMTHEEGMMKMREVKELALPAGKRVSLGESGY
                     HLMLIGLNAPLKAGEAVPLTLTVKLANKRIVKVEASAEVRPLLASAGQDGAHMHHHQH
                     "
     gene            11163..12488
                     /locus_tag="VFW59_02055"
     CDS             11163..12488
                     /locus_tag="VFW59_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5363025.1"
                     /translation="MNHIPFAAQTGHSHLRRLVWLRTITVAAQCAALVLVYRFLKMEL
                     QWSPMITAIVALSALNLATWLRLKYHSKPVSAVELFTQLCADVLALSVLLYFSGGSTN
                     PFISLYLLPLVIAAATLPHGYTWAMTALTTACYTLLMKYYVPLPMHHEMTGNMSGMEG
                     MDHDMSAMNVSDLPQESIFNLHVFGMWLGFVISAVVIAYFVVKMAHEVRERDAALARV
                     REETLRNERIVALGTQAAGAAHELGTPLSTLAVVIGELKREPSMLPEWRADLDILDGQ
                     VQACKRILDKLLAHAQDHETAQTLEQFLAETLSEWQLLRPTAHYRYQAPSAHSAPHLH
                     IDPALRAALLNLLNNAADASPDEIDIEAHWTAENFMLSIHDHGLGLTPEAIARAGSAF
                     FTTKEEGRGLGLFLANATVERMGGKVRLFNREVGGATTEIILPLQTRRS"
     gene            12485..13033
                     /locus_tag="VFW59_02060"
     CDS             12485..13033
                     /locus_tag="VFW59_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5363026.1"
                     /translation="MSTLPNEQASLLLVDDDPTFCRVLAGALEKRGYSVCIAHSVEQA
                     IPLAEANPPEFAVVDLKMSGVPGLTLVKALHELDPNTRIVMLTGYASIATAVEAIKLG
                     ATQYLAKPANADEIVAAFSHVPDDSAPLRTQPTLIGNLEWEHIQRVLNEHDGNISATA
                     RALNMHRRTLQRKLGKPPSHPG"
     gene            13130..13999
                     /locus_tag="VFW59_02065"
     CDS             13130..13999
                     /locus_tag="VFW59_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5363027.1"
                     /translation="MTDDDTRARLKHSLANLESLPAMPSIAQKLLALPLDTEEGEMEL
                     LSLIEQDPQISAKLIGLANSPIMGVMRKVSTVHDAAMLLGLTRVKSVSIGIATMSDFS
                     RLPASRNFKPQDLWLHCMITAVVMRTIAAAMPARIRPPEDQIYLAGLLHDIGFMALHH
                     LDHELSNELHRQLHLQPERPVLEIELETLGITHCYIGSQIARHWNLPKEIGEVLGYHH
                     PPYVDEMPEQNLLARLVSVAEKLQPDFGISEHTGDEVHAHEWAELGIELSAVEEIGIQ
                     ANELAIQATQIAI"
     gene            complement(14031..14456)
                     /locus_tag="VFW59_02070"
     CDS             complement(14031..14456)
                     /locus_tag="VFW59_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HEX5363028.1"
                     /translation="MALLAEVKEFFGLSATQSVSYRDLNLLGAAEAHILWKARLGHHI
                     EGNVREPLESVLVGQGGFCQLGCWITGPELEPFRELPDFVLLSEAHQQFHHIGTLIIE
                     KIKAGERAAAREIFRNEYNQALRAIIQALTGISRHVQAK"
     gene            14651..15787
                     /locus_tag="VFW59_02075"
     CDS             14651..15787
                     /locus_tag="VFW59_02075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5363029.1"
                     /translation="MRSVLLFAGLLLSSAVFSAPAPIPVAPQLAAKAYLLYDYTSNQI
                     LVNKNGDTRMEPASLTKLMTAYLAFDALKHGTLSSDQTLTVPATALRAKGGESRMLLK
                     AGQEVTVDELLRGLIVQSGNDAAITLAVNIAGSEAGFVDMMNKEAKRLGMNNTHFTNA
                     IGLPDAQHYSSASDLALLTAAVVHDYPQHQQLFALREYSFNNVTQANRNRLLWMDPFV
                     DGMKTGHTETAGYCLIGSAKRNDRRLISVLLGADTDNLRSVESQKLLNFGFQNFDAVQ
                     LYKKDQPATQLRVWKGTERQVAVGFRNDLFLTIPKGKLAQLKASVETRQPILAPISSG
                     QQLGTLKLSIAGQPYAEYPLIALDAVPLANVLSRGWDSIRLFFQ"
     gene            15797..16651
                     /locus_tag="VFW59_02080"
     CDS             15797..16651
                     /locus_tag="VFW59_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEX5363030.1"
                     /translation="MTIYLNGAFMPVEEAKISVLDRGFIFGDGVYEVIPVYSRRAFRL
                     REHLRRLQHSLDGIKLANPHSEEDWITIVEQIIERNPGEDQYLYLHITRGAAKRDHAF
                     PNPPVAPTVFVLSSLLAQPSAEQLSSGISCITATDNRWLRCDIKAISLLPNVLLRQMA
                     VDAGCAETVLIRDDSFLTEGAASNIFVVKNGALLAPPKDNLMLPGITYDVILELAAAN
                     GIPCEVRRVMKAELFSADELLLTSSTKEVLAITRLDGQRVGSGKPGAMFARLHQLYQD
                     FKHNVMRK"
     gene            16648..16932
                     /locus_tag="VFW59_02085"
     CDS             16648..16932
                     /locus_tag="VFW59_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEX5363031.1"
                     /translation="MNDTVKNELTHSLIEYPCDFPLKIFGQQQAGFAQAVLEVVTKHD
                     PGFLASSMEMRSSKNARYVSLTCTIRATSREQLDAVYQELCDHPMVVMVL"
     gene            16932..17600
                     /gene="lipB"
                     /locus_tag="VFW59_02090"
     CDS             16932..17600
                     /gene="lipB"
                     /locus_tag="VFW59_02090"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX5363032.1"
                     /translation="MASGELEAGSGKHPATPAIHALGMVDYQPTWEAMKRFTAERTEE
                     TRDEIWLVQHPPTYTQGLAGKPEHLLNPTAIPVVKIDRGGQITYHGPGQILAYLLLDM
                     RRWKIGVRELVRLMEQAVIDLLGEYGVAAHSREEAPGVYVNGAKIASLGLKIKNGYCY
                     HGLALNVDMDLSPFLNINPCGYAGLRVTQASELGVAAPINELQAELAQNLIHGLQQHL
                     ARKP"
     gene            complement(17597..18202)
                     /locus_tag="VFW59_02095"
     CDS             complement(17597..18202)
                     /locus_tag="VFW59_02095"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEX5363033.1"
                     /translation="MKTITLALTGASGLPYGMRLLECLLKAGVRVQLVYSQAAQIVAK
                     QEMDIVLPSRPQDAEKLLAERVGEFSGELRVYGRDDWYAPMASGSNPGDAMVVCPCTM
                     ATLGKIACGIGDDLIARAADVMLKERRPLVLVPRETPFSAIHLENMLRLSHAGAVILP
                     PSPGFYHHPQSVQDMVDFVVARILDHLGVAHSLQQRWGGED"
     gene            complement(18199..18681)
                     /locus_tag="VFW59_02100"
     CDS             complement(18199..18681)
                     /locus_tag="VFW59_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEX5363034.1"
                     /translation="MAAVALGLTVLESAIPSPLPGVKPGLANIVTLIVLARYGWRVAV
                     WVSLLRVLAGSLLFGNFLAPGFFLSLSGALCSLLVLAFARQLPSRWFGVVSHSIYAAF
                     AHVAGQMAVVYLWLIPHAGIAYLIPIFATAALVFGTVNGLIAARFMDNDAERSPESAA
                     "
     gene            complement(18812..19210)
                     /locus_tag="VFW59_02105"
     CDS             complement(18812..19210)
                     /locus_tag="VFW59_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEX5363035.1"
                     /translation="MSSHEAVRAAILPHLKPGDWLTLLFGSLCVVLLMRELWSGDLAD
                     KAIIRSGGKIFREVPLSRDQQIEVPGPLGVSIVSIRQHKARISSDPSPRQYCVRQGWL
                     AQSGEIAICLPNEVSVELTGSRKKYDSLNY"
     gene            complement(19197..20033)
                     /gene="rapZ"
                     /locus_tag="VFW59_02110"
     CDS             complement(19197..20033)
                     /gene="rapZ"
                     /locus_tag="VFW59_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX5363036.1"
                     /translation="MQLILISGLSGSGKSVALKTLEDAGFYCVDNLPAELLPNLIDQL
                     LPTGYRNIAVSIDVRNAHSVQVLPSLMLQLMQKELAVHLLYLDARTDTLVKRFSETRR
                     LHPLNDGVKTLPECVAHERELLAGIGGIGHHIDTSDLNANALRAWIKRFIPLDRARLT
                     LLFQSFGFKHGIPLDADLVFDVRCLPNPHYDERLRPLTGRDAAVADFLEASEQAQKMY
                     ADIRDFVANWLPCYIADSRSYLTVAIGCTGGQHRSVYLAERLARHFEGQQQVLVRHRE
                     LA"
     gene            complement(20045..20983)
                     /gene="hprK"
                     /locus_tag="VFW59_02115"
     CDS             complement(20045..20983)
                     /gene="hprK"
                     /locus_tag="VFW59_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX5363037.1"
                     /translation="MSEVNVRQLFEDKQARLELSRVAGADGNDRIISADEVNASNKGL
                     IGHLNLIHPNWVQVLSETELNYLRGLSASELEAAFRQIEQSSPTCLIVAGAHDIPAEL
                     LDFANRTHIPLFRSSKPSVHLMWLIRHYLAKELAESTTRHGVFLDVLGVGVMITGESA
                     VGKSELGLELITRGNGLVADDIVELHRIAPDTLEGRCPELLRDFLEVRGLGVLNIRTM
                     FGETAVRRKKSLKLIVHLHRPPGGDLSQVERLPINASYQEILGVHVSTVNIPVVAGRN
                     LSVLVEAAARNFVLQQRGIDSMQEFILRHDQLLQND"
     gene            complement(20980..21441)
                     /gene="ptsN"
                     /locus_tag="VFW59_02120"
     CDS             complement(20980..21441)
                     /gene="ptsN"
                     /locus_tag="VFW59_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEX5363038.1"
                     /translation="MNLISKILAPDNILLDTESTSKKRVFERAGLLFENNQQIARSQV
                     FDSLFAREKLGSTGLGHGVAIPHGRIAKLRDATAAFVKTSHPIPFDAPDGEPVNLIFV
                     LLVPERATDQHLQILGELAQMFSDSQFRNRLLACEDEPSIHQLFYDWNGSL"
     gene            complement(21488..21811)
                     /gene="raiA"
                     /locus_tag="VFW59_02125"
     CDS             complement(21488..21811)
                     /gene="raiA"
                     /locus_tag="VFW59_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5363039.1"
                     /translation="MNINLTGRHLEITPAIREYANGKFGKIKRHFDNVIDVNIILSVE
                     KLKQKAEATVHISGHDVFVECEDENLYAAIDTLVDKLDRNVLKHKEKLAARRHDDSGK
                     PAAAE"
     gene            complement(21828..23267)
                     /locus_tag="VFW59_02130"
     CDS             complement(21828..23267)
                     /locus_tag="VFW59_02130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX5363040.1"
                     /translation="MKHALQLRTSQQLTLTPQLQQAIRLLQLSTQDVHQEIERMLDEN
                     PMLELADDYVPAMSPVSDVRSEAISDQPEAGTSEDSGEERLGAEDFALSAADWEGRER
                     GSSADDEDEGRPEQAAEQISLREHLHEQLATGSLEDRDRIVVGLLIEAVDENGYLAQD
                     LVELAELLPAELDITLDDLETALVQLQHLDAPGLGARNLAECLTLQLKALPEDTPQRD
                     LAIHLASQYLDILAAHDFAKLKKLLHCSDDELRLAQHLIVGMNPKPGAEFDHRVADYV
                     VPDVIVEKHQNKWRARLNAEAMPKLRVNQVYAHILQQRDEGNGSQLATRLQEAKWLIK
                     NLQQRFETIQRVAQAIVERQEKFFEHGEIGMRPLVLREIAEELELHESTISRSTTQKF
                     MLTPRGIYEFKHFFGSGLVTESGGTCSSAAIRELIRQLVSAENTRTPLTDSRMSEILA
                     QQGIVVARRTIAKYREALHILPVNLRKSL"
     gene            complement(23300..24022)
                     /gene="lptB"
                     /locus_tag="VFW59_02135"
     CDS             complement(23300..24022)
                     /gene="lptB"
                     /locus_tag="VFW59_02135"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX5363041.1"
                     /translation="MSELRAVGLKKKYGSRTVVHDASLTVRGGEVVGLLGPNGAGKTT
                     SFYMIVGLVAADGGEIFIDDQNITHLPIHRRARLGLGYLPQEASIFRRLSVAQNIQAV
                     LELQGLADEALQNRLDELLEDLHIDHIRDNQAVSLSGGERRRVEIARALATDPRFILL
                     DEPFAGVDPIAVLDIQKIIHFLKERGIGVLITDHNVRETLGICDRAYIINAGAVMAEG
                     SPDEIIYNEGVRKVYLGEHFKL"
     gene            complement(24019..24591)
                     /gene="lptA"
                     /locus_tag="VFW59_02140"
     CDS             complement(24019..24591)
                     /gene="lptA"
                     /locus_tag="VFW59_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX5363042.1"
                     /translation="MSHARLLLLGLLLSQPCLAERADRDKPVHIEADQVLVEDAQKVD
                     TFTGNVQMTQGTMLIRGDKIVVTEDAEGFQHATAYGQTASFRQKREGLDEYVEGYGER
                     IEHDTRAETVDFYVRARVVRKLDEVRGEHITYSQKTEVFRVNSGAVAGGPPKRVRAIL
                     QPKPREGAASSVPEALPIAPSDTLAPAEAE"
     gene            complement(24578..25153)
                     /gene="lptC"
                     /locus_tag="VFW59_02145"
     CDS             complement(24578..25153)
                     /gene="lptC"
                     /locus_tag="VFW59_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX5363043.1"
                     /translation="MTFYSRARYWLPLLPLLGILGVTYWLDQQTHLEPETAGGGMRHD
                     PDAIMENFSSVKMNDQGVPRFIMSAKKMQHYPDDDSSALEAPRMASLSATLPTVHLVA
                     QRGIVSGSGNEVFLHDEVEVLREASATRGELTLQTDYLRVVPDRDWADTDHAVTIFDE
                     RNVVHAVGLEMDNKARTLKLLSQVRSVHEPR"
     gene            complement(25150..25668)
                     /locus_tag="VFW59_02150"
     CDS             complement(25150..25668)
                     /locus_tag="VFW59_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX5363044.1"
                     /translation="MLLSRAKLIRLMTFDVDGVMTDGGLFLTDSGDEMKRFNSLDGHG
                     IKMLRASGVEIAIITGRTSRCVELRAQNLGIEHVYQGVEHKLDAMVDLLNRLKLSRDA
                     AAYMGDDVVDLCVMRHVGLSISVPESPQVVRNHSDYVTQRSGGHGAVREACELIMSAQ
                     GTLDAQLAPYLK"
     gene            complement(25698..26684)
                     /locus_tag="VFW59_02155"
     CDS             complement(25698..26684)
                     /locus_tag="VFW59_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX5363045.1"
                     /translation="MNKSLSAAPRALDLAREVLSIEAAAIQSLVTRLDETFLRALDVI
                     LRCEGRVIVSGMGKSGHIARKIAATMSSTGTPAYFVHPGEASHGDLGMITEEDVIIAL
                     SYSGESQELMTIVPVIKRQGAKLISMTGNPDSSLAMAADVHLNAAVDKEACPMGLAPT
                     ASTTAALALGDALAVALLDAKGFGAEDFARSHPGGSLGRRLLTHVRDIMRSGERLPMV
                     GEAAMLGEALLEISRKGVGMTAIVDAGQHVLGIYTDGDLRRTLEKKLDFGSTPVRSVM
                     SAKPRCIGPDALAVEAVQMMEQYNISQMLVVDEQHKLVGALNMHDLLHAKVI"
     gene            26825..28792
                     /locus_tag="VFW59_02160"
     CDS             26825..28792
                     /locus_tag="VFW59_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX5363046.1"
                     /translation="MESTLSLVLILLATAVMVVVLCRILHLPVMLGYIAVGILIGPHA
                     LAWIPDAMGTQHLAEFGVVFLMFSIGLEFSLTRLLAMKRTVFGLGGAQVLVTILLTMA
                     IAALFKLDWRTGLALGGVLAMSSTAIVSKMLVERAELNLPYGQQMMGILLFQDLAVVP
                     LLIIIPALASNPDDLPATLAFATLKAIGVLLVLLVFGQRIMRSWFHVVARQKSSELFM
                     LNVLLITLGLAFFTELAGLSLALGAFVAGMLISETEYRYQVEEDIKPFRDVLLGLFFV
                     TIGMKLNLGAVVADFGWILLTMTGLILLKALVVALLARTFEGDWGVALRSAIGLAQAG
                     EFGFVLLTLADAHLLNDATMQIVLAAMLLSMLAAPFLIQHGEAIARRFSASEWTNRAM
                     QMHQIAVQSMGRSAHIIVCGYGRSGGALASFLKGENLHFIALDLDSRRVRQAVTDGES
                     VVYGDAAKREVLMAAGLMRAKALVITYSDKHSALAILRHTKELRPDMPVIVRTHDDSH
                     VEELKAAGAAEVVAEVLEGSVMLASQALLMAGIPLNRVIRRVQESRARRYASFSGYLR
                     TVRDDVGEITESQQSRFLSVLLKEDSTAVGKQLGSIGLDALNVEVNAVRRHNLRGSQP
                     SPDMVMQAGDVLVLFGLLEALQLAEVKLHQG"
     gene            complement(28773..29207)
                     /locus_tag="VFW59_02165"
     CDS             complement(28773..29207)
                     /locus_tag="VFW59_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5363047.1"
                     /translation="MDASRRGRPLTLGLRNIIFSYARSAEMPNRNLVRASIPQHRKMP
                     PVKNETAPTTAPLKGNWLCYLLRCADDTLYCGITNDLSKRLAAHNAGTAAKYTRARGP
                     VALVYVEPCDDRSAASKRELAIKRLTRAEKLLLIAALSPDAA"
     gene            29281..>29598
                     /locus_tag="VFW59_02170"
     CDS             29281..>29598
                     /locus_tag="VFW59_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5363048.1"
                     /translation="MSVETPARACGIDFGTSNSTAGWLRPGQSPLLALEDGKITLPSV
                     IFFNADEDTVSVGRAALNEYMEGYEGRLMRALKSLLGSSLMEGQTEVQGRPLPYKALL
                     AQFI"
BASE COUNT         5976 a         9567 c         8363 g         5692 t
ORIGIN      
        1 tgctcggcat gtgcccgttg ctggccgtca gcacctcggt ggtcaacggc gccagcctcg
       61 gtttcgccac cgccgtggtg atggcgctca gcaatgccag catctccccg atccgcaact
      121 tcgtcccgaa cgagatccgc atcccggtct tcatcctgat catcgccgtg ctggtcaccg
      181 tggtgcagta tctgatgaac gcctacatgt acggcctgta cgtgctgctg ggcatcttca
      241 tcccgctgat cgtgaccaac tgcatcgtgc tggcacgggt cgaagccttc gcctcgaaga
      301 acccggtctt gcccgccgcg ctggacggcc tcgcgatggg actgggcctc accgccgtgc
      361 tcggcacgct gggcggcatc cgcgaactgt tcggccacgg cacgctgttc tccggcatcg
      421 agctggtgtt cggcgaagcc gccaagtcat gggtgatcac cgtggtgccg gactatcacg
      481 gcttcctgct cgcagtgctg ccgcccggcg ccttcatcac gctgggactg ctgatcgcca
      541 tgcgcaactg gctgaccctg cgcgccgcac aaaagacaaa cggcgcggcg gccagcatcg
      601 ccccgatcga aggcggctgc tcgaccactc acacggcatg aacgccgcga agcgtcgcga
      661 gatattcctg cgcctgcagg ccgcgaatcc gcaccctgtc accgagctgg agtaccacac
      721 cccattcgag ctgctgatcg ccgtgatgct ctcggctcag gccaccgaca tcagcgtcaa
      781 cgccgccacc cggcaactct atccggtggc gaatacgccg caagcgatac tcgatctggg
      841 cgaagcggga ttgcgcggat acatccagcg catcggcctg taccagacca aggccaagca
      901 cgtgatccag acctgccgcc tgctgctgga atgccacgcg ggcgaagtgc cgcaggcccg
      961 cgaggaactg gaagccctgc ccggcgtggg acgcaagacc gccaacgtga tactcaacac
     1021 cgccttcggc cagccgacca tcgcggtaga cacgcatatc ttccggcttg ccaaccgcat
     1081 cggcctggcc cccggaaaga ccgtgctggc ggtcgagcag aagctgctca aggtgatacc
     1141 ggatgaattc aaacacgatg cccatcactg gctgatcctg cacggacgct acatctgtca
     1201 ggcgcgcaag ccgaagtgcg gccagtgcat catccacgac ctgtgcgaat acaaggacaa
     1261 gcaggattga ggagcgagga ttaagtcaaa acagtgagcc gcaattcatc ctatatttac
     1321 gaaagaacac gatgctattc aatccctcgc gcgacgaggc acgcaacttc ctgatcgaat
     1381 cctggcgcaa gcgccgggcc aacgaattgc tgacgccgct ggaagatctg gccgcgcagc
     1441 tgatcgccaa gcaccccgag caccatgccg tattcgaaga tggccagctc gaccgcgact
     1501 acctgccgga acatggcgcg accaatccct tcctgcacct gatgatgcat ctgaccatcg
     1561 aggagcagat ctccatcgac cagccgccgg gcatacgcgg tcatttcgcg cgcctgacgc
     1621 gcaaattcga atcggaacac gaggcccagc acgccatgat ggaatgcctg accgagatga
     1681 tctggcaggc gcagcgcgat cgcacccagc cggacgcggc aatttatctg gattgcctcg
     1741 tcaggcagca ataaagggta aaatacccat cctgatgttg cgatacacag ccatcgaata
     1801 agaaacaccg tgcaccccaa acttccaaaa cgcttgctac aaaatttcct gcccgccgcg
     1861 ctgatcatcc tggcgctggg cggcgtcttt ttcgagttcg aatcggccga agacctcaga
     1921 accctgatcg accatgaaca ggatcatgtg catgcgggtg tggaaaccgc caaccgcaca
     1981 ctgctcacgc tcaaacgcga cacccagaat ctggcgcact cgaccacgct cggcgaagca
     2041 atcttgcagc cgagtcgcac ttcccgactc gagcaggaca tgctcgcttt catttcgagc
     2101 aaagtcgtct actacaaggc acgctggatc gacgagaacg gtcaggagcg cgcccgggta
     2161 gacatccaga acggcaaggc tgtcatcgtc ccgagcagtc gcacagagaa caaagccaca
     2221 cgcgactact ttgcgcagac gattaagctc agcgacggcg caacctatct ttctcctctg
     2281 gacctggaga tcgagcatgg cgccatcgaa cagccgttgc gtcccacgct acgcgccgcc
     2341 accccgctgt tcgatgctgc cggccgcaag cacggcctcg ccgtcgtctc ctactcggcc
     2401 caagacctgt tcaggcgcat ccaggaaatt tccgcgaccg agcactcttc gtggatgctg
     2461 ctcaacgaac agggttactg gctaagaagc ggcgatccgg cacaggaatt cggtttcatg
     2521 ctgccgcatc gtgccagcat ggcggaacag caccccgaga tatgggatcg catcaaggcc
     2581 gcgccagtcg gcaacttcat cgacgcagat ggcggcctgt ggctattcga aacgatttac
     2641 ccccatcgag tcgtcaacga tcaggggacg caggatgatg gcgaggcaag ctactggaaa
     2701 atggtcaaac acttcgatgc ggcagaactc ggcggcatgc aacagtatcg acgcacacag
     2761 accttgattc tggtcagctt cgtcatggca ctggccctcg ccatcagttt ccgcctgagc
     2821 cgctcgcaga tcgacaagga agaccgggac atcgacctgc atcatctgct cggcgagctc
     2881 aaccagcagc agttcgcact cgatcaacac gcgatcgtcg cggtcgcaga tctgaatggg
     2941 acgatcaact atgtcaacga caagttctgc gccatcagcg gctaccgcag ggaggaactg
     3001 atcggacaga accatcgcat cgtcaagtca ggacaacaca gtaacgcgtt ctatcgcgag
     3061 atgtccgaag ccatcaagca gggcaaggta tggaatggcg aaacctgcaa ccgcgcgaag
     3121 gatggcagcc tgtactgggt gaagaacacc ttcgtgccct tgctcagcgc tcgctcctat
     3181 atcgccatca gcaccgacat caccgcccag aaggccgtcg aagaaaaact gcgcatcgcc
     3241 tccatcgcct tcgagaccca cgaggccatc gtcgtcaccg atgcacaggc acgtatcgtc
     3301 agcgtcaacc gggcctttga acaactgacc ggctacagcg ccgaagaggc cctcggccag
     3361 aatccgcgca ttctgcagtc cggccgccat gacaaggagt tttacacgga aatgtgggcg
     3421 accctgctag tgaagggggt ctggtccggc gagatgtggg atcagcgcaa ggacggctcc
     3481 gcctatccga agtggctgac catcacctcc gtgcgcaacg acaagaatga aatcaccaac
     3541 tatgtggcga acttcatgga tatcagcgag cgcaagcgcg ccgaggatga aattcagcgg
     3601 ctcgccttct acgacaccct gaccgaactg ccgaaccgcc gcctgttgat ggacaggctg
     3661 atccagtcgc tgttgaacag cgaacggagc ggagcctacg gcgcgctgct gttcatggac
     3721 atggacaact tcaaggtgct gaacgacacc cagggacacg atgtcggcga catgctgctc
     3781 atcgaggttg cgaaccgcct caggggctgt gtacgcgaaa gcgacaccgt ggcgcgcctc
     3841 ggcggcgacg aattcgtggt cattctgcag gggctgggaa acagcgagat cctggccgcc
     3901 aatcaggccg aagatcttgc cgaaaagatc gtcgagtcgc tcagcaaacc ctatttcctt
     3961 gcggcgcacg aacaccatag cagcgtgagt atcggcgtcg gcctgtttct gggacgcggc
     4021 accaaggtcg acgaactgct caagcgcgcg gataccgcca tgtatcaggc caagtcatcc
     4081 ggacgcaatg cggtacgctt cttcgaatcc gccatgcaga atgcagtcga atcgcgcgcc
     4141 atcctcgaaa aacagctccg tcaggccttg tccaggaacg agttgcaact ctattaccag
     4201 atccaggtta atcaggagcg gcgagtcgtc ggcgccgagg cgctgttgcg ctggatcaat
     4261 gaggaacaag gtttcatctc gcccgcccag ttcatcccgc tggccgaaga aagcggcctg
     4321 atcctgccga tcggcctatg ggtactggat accgcctgcc agcaactgaa gcagtggcag
     4381 gattcgcctc acgcccgcca tctgcagctg gcggtgaatg tgagtgcgcg gcaattccgt
     4441 cagcctaaat ttgtcgagca ggttaaaaac ctgctcgaca aatacgccat cgatccttct
     4501 cgcctcaagc tcgagttgac agaaagcgtc gtgctggccg atgtggacga gacggtgcag
     4561 aaaatgctcg cattgaaaca gtttggcgtg caattctcga tggacgattt cggcaccggc
     4621 tactcatcgc tgagctacct gaaacaattg cctttggatc agatcaagat cgaccagtcg
     4681 ttcgtccgcg acatcgtcgt ggacaagagc gatgcggtca tcgtcaagac catcatcgac
     4741 atgtcgatca acttcaatct ggaagtgatt gcagaagggg tcgagaccga agaacaacac
     4801 gagatactgc ggcagaacgg ctgccatgcc ttccagggtt acctgttcag caagccgatc
     4861 ccgatcggag aattcgaaca gctcatctcc cgtaacgcat gagtccaaag cgataaatca
     4921 tgtcgcgcaa tagaccgcgc cagacagatt cttcggcagc agactaactt cccagcatct
     4981 ccgcgatctc gcgctcggct gccgcccaat gctccgtcga gttgccctga aaaccatggc
     5041 gctcggcgat gaaataagcc gccatctcca ccatccggta acgctcttca ggagagggct
     5101 tcgccttgga cgctgccgac ttcttggctg gcgccttctt ctcaacagca ggcgctgcgg
     5161 tcttcttcgc tgcggcgtcc ttctttaccg ctgacgttgc aatctttttt tccactgaat
     5221 ccaccttctt tgctgctact ttcttcgcaa ccggtgcctc ttttgcagcg ctcttcgact
     5281 tggttaccgc agccgttttt ttgactacag ccgcctttgc tttttgctct accatgatgc
     5341 ctccttggaa ggtaagacta acgagtgaat attcactgcg ggccatgata tggcccggca
     5401 gacggcgggt caacacattc acggagtctc gtcttgacgt tgcaaggctc agcggctagt
     5461 gtgcatgcac gctattcacc gcaaacgcaa atgaaacacc tgaaatccga atacccgatc
     5521 gccctcgccc tcgccgtact ggggctgggt tttgcgctcg aacacagcgc cgccgaacag
     5581 ggcggcgcca tcctgtgggg cctgctgctg gtcattctcg ccgccgtgat cggcgtggcg
     5641 ttccgcatcg cccaccacgc cgaagtgctg gccatacgct ttggcgaacc ctatggcacg
     5701 ctgatcctga ccctcgccgc cgtctccgtg gaggtggtga tactggtggt gctgctgagc
     5761 ggcgaaccca acccgacgct ggcgcgcgat acggtattcg ccgccgtgat gttcgacatc
     5821 aacggcatac tcggtctggc agccatcgtc ggcggcctga aacacggcag cagcaaatac
     5881 aacgtggact cggccaattc gttcatcgcc atgctgctgg tcgccatcgg catcggcatg
     5941 ttcatccccg acttcgtgcc ggaagaaaaa tggcaggtct actcggtatt ttccatcggc
     6001 atcatggcaa tcatgtatct ggccttcctg cgcctgcaga ccgtggagca tcgcaagttt
     6061 ttcgaatact ccgccgagac cgatgaagag gcgcacgacg agacgcacag cccgaatgcg
     6121 ctgcatgtcg gcgtgatgct ggccgcgatc gtactggtcg gcctgctgtc cgaagtgttg
     6181 tcggtgctgc tggatgcggg cctcgccggc agcagtctgc ccaaggccat ccccgccgtg
     6241 ctggtggcgc tgatctccgc cagcccggag attctcaccg cactcaaggc cgcacaacag
     6301 gatcgcatgc agaccacggt caacatcgcg ctgggcgcgt cgctggccac cgtgctgctc
     6361 acgctgccgg tgatcgaggc catcgccctg ctcagcggcg aacgcatcga gatggcgctc
     6421 acaccggttc aggcgggcat gctgctgctg accttcctca ccgtgatgaa caacctgcac
     6481 gacggcgaga ccaacgccat cgagggcatc agccacttcg cgctgttcgc cgccttcatc
     6541 gcgctggtga cgataggctt gatctgatgt tcgcgctgga aacctgggag ctgctgagtt
     6601 atgtcgtgac cgtgatcggc ctgccgctgg cgatcgcggt gttcgtcttc gaacagcgca
     6661 aggagcgcga caacgaagag gaagaggtct atcagctgct gtccgacaac tatcaggagt
     6721 tcctcaagat cgcgctgacc aatcccgacc tgcgcctgtt tgccacggaa gaatcctccg
     6781 tacgcacgcc ggaacagagc gagcgcatgt tcatcatctt cagcatgctg atctcgctgt
     6841 tcgagcgcgc ctacctgctg ctctacgaag acggcatgaa cgagaagcaa ttgcgccgct
     6901 ggcgctcgtg ggaagactac atgggcgaat ggtgcgaccg cacggatttc cgcgccgcgc
     6961 tgcccgcctt attgcgcggg gaagacccgg agttcgcggc ttacatccag cgtctggcgt
     7021 cgcgtcattc ctgacgctgc cgcaaggcga cgcgcttgag gtaagcctcg cgcgcgatct
     7081 gcacatcttc cagaaagtac ggcagctcct tcagcagcat cgcctgcggg ccgtccacca
     7141 gcgccttcgg gggagccgga tgaaagtcca ccagcaccat gttggctccg gccagcacgc
     7201 cctgggctgt gacatgcatc atgtctagga tgccgtccgg cgaggcggcg cgggaaccaa
     7261 ccgaatgcga ggggtcgatg cacaccggca tgcgcgtcag gcgtttgacc accggcacat
     7321 gggcgaaatc gacgaagttg cggtgcggat cgcccatgtt ggtcttcatg ccgcgcagcc
     7381 cgaagatcac cttgcggttg ccctcggaag caagatactc cgccgcattc agcgactcgt
     7441 ccagcgtgat gccgaagccg cgcttgagca tgatgggcat ctcggtctga cggccgacga
     7501 tcttcagcag ctcgaagttc tgcgtgttgc gcgtgccgat ctgcagcatc acgccggtcg
     7561 ggttgcccgt ctgttcgagc gcagcgcgga tctcgtccag atgggattca tgggtgatct
     7621 ccatcgcgat caccttgatg ccgtactttc cggccagctc gaacacatag ggcagacaat
     7681 tcttgccgtg cccctgaaac gcatagggac tggtgcgcgg cttgtaggcg cccatgcggg
     7741 tgcacacctg cccggcgtca cgcacggcct tcatcatgat ctcgacatgc tcgcgattgt
     7801 ctaccgcaca cagccccgcg aacacatgca gcgtgtcctg accgaaacgc acgccgttgt
     7861 aatcgaaatg ggtcggacgc tgctcgtcct tatgccgccc gaggatgcgg tactcctcgg
     7921 agacgcgcac cacccgctcc acgcaaggca ggctctgcat ctcgccgata tccagcgcct
     7981 tggtgtcgcc gatcagatag atctcggtca gattctgctc gacaccccgc tcgacatgca
     8041 cgcgagtctg gatgccctgc agggtcgcca gatgcgccag cagctgccgg tattcgggcg
     8101 actgctcgcc cgcgttggca tcgagaatca gaatcatttt cttctccgct tatttctgca
     8161 gcagaatcgc ggactgcgcc tgccactgcg ccagcggatc gcgcgtgaag ccgctcttgg
     8221 cgaactgctg ccagcgcccg atcacgtagc acaccagcag gttcgcatgg gcgccgacat
     8281 ccaccgcctc gcccatctcg ccctgcgtgg cggcgatgcg cagcgactgc ttgagcgtcg
     8341 cctcgacgcg gtccagcagc tggttgatac ggagctgcaa acgctcgtcc tcgttcacca
     8401 gcgcatcgcc gatcagcacc cgcgtcatgc cgcgattctt ctgcgcgaag ccgagcagca
     8461 gcgacacgat ggactcgacc tgctgcaagc cgctcttctc gtcctgggtg atccggttga
     8521 tcaggccgaa cacggtctgc tcgatgaact cgatcagccc ctcgtacatc tgggccttgc
     8581 tggcgaagtg gcggtacagc gccgcctcgg aaagctcaag tcgcgccgcc aacgctgcgg
     8641 tggtgacctt ctcgcccttg ggctgctcca gcatgtgcgc caccgtctgc agtatctgta
     8701 attttctttc gcccggtttg cttgccatat tcacctcacc tgtttaaacg atgcagtgcc
     8761 ctgggcagct gcatcacgtc tcgtatcttc acatccacgc agggcgcgcc ccggctgccc
     8821 gcattcaccc acaccgtgcc cattcccagg cgtttggccg cctgcagatt ctccaggctg
     8881 tcttccacca tcacacactg cgccgccctg atgcgatgac gccgcagcag acgcaggaag
     8941 ccgaaactgt ccggcttggg acgatagcgt gtctgctcca ccgccatcac gtcgtcaaac
     9001 agatcgtcca cccgcaacag ccgcaacacc gcatgcgcat aatgctgcgg ggcattggaa
     9061 aacaccatct tcttgcccgg caaattcttc agcacatgcc gcaggcgcgg ttcgcgcaac
     9121 accatctgcg gcagttccgg aaactggtgc gtgtgccaca ggaagtgatc gggatcggta
     9181 ccgtgatgca gcatcatgcc gctcagggtc gcgccgtaac gctgccaata atccatccgc
     9241 aacgcattcg cctcatcctc atccagttgc agatgctgct gcaaataggc ggtcatgctg
     9301 cggttgatat gcggaaaaat atgcggcgac gcatcgtgca gcgtgttgtc cagatcgaaa
     9361 atccagacga ttccgcccat cacttgctct tgatcagcgt gcccacgcct tcgtcggtca
     9421 gaatctccag caacagcgcg tgctccacgc gcccgtcgat gatatgcacc gacttcacgc
     9481 cgctgcgcgc ggcatccagc gccgaactga tcttgggcag catgccgccg gacagggtgc
     9541 cgtcggccac cagatcgtcg atattcttcg gcgtcaggcc ggagagcaga ttgcccgcct
     9601 tgtccagcac gcccggcgta ttggtcagca gcaccagctt ctccgccttc agcacttcgg
     9661 ccagcttgcc ggccaccaca tcggcgttga tgttcagcgt ctcgccgtcg gccgcgacgc
     9721 cgattggcgc gatcaccgga atgaagtcgc cggaatccag aaaggtgatg atggcaggat
     9781 cgatcttggt gatctcgccg accagcccga tgtccagcat cttgcccggc tcgtccttgc
     9841 tctccagcat catcttctcg gcgcggatga atgcaccgtc ctgcccggtc agtcccaccg
     9901 ccttgccgcc gtgccggttg atcaggttga cgatatcctt gttgaccttg cccagcacca
     9961 tctcgaccac atccatggtc tcttcgtcgg tgacgcgcat cccctgtatg aaggtgccct
    10021 gcttgccgac ccggttcagc agatcgttga tctgcggtcc gccgccatgc accaccaccg
    10081 gattcatccc caccagcttg agcagcacca cgtcgcgcgc aaaactatcc tgcaacatgg
    10141 gatcggtcat ggcattgccg ccgtatttca ccacgatggt cttgtcgaaa aagcgctgga
    10201 tatagggcat cgcctcggac agcgtgcggg ctttcttctc ggcggcagag ggcgtaagag
    10261 acatgaattt tcctcggaat ttaaaggggc agcaggcttt tccccctgag ggtgaaagct
    10321 cacagaattt ggcgcgaatt ctacaccaga aagcaaacag gcggcatacg ccgcctgttt
    10381 ttcgggaaga gcaaacactc actggtgttg ccggttccgc tgctgactca gtgttgatgg
    10441 tggtgcatgt gagcgccatc ctgcccggca ctcgccagca gtggccgcac ttctgcgctg
    10501 gcctcgactt tgacgatgcg tttgtttgcc aattttacgg tcagcgtcaa cggcaccgcc
    10561 tcacccgcct tgagcggcgc attcagaccg atcaacatca ggtgataacc gctctcgccc
    10621 agactgacac gcttcccggc aggcaatgcc agctccttga cctcgcgcat tttcatcatg
    10681 ccctcttcat gggtcatgct gtgcaactcg acgcccttgc aggccgggct ggacaggccg
    10741 accagcgtcg catcctgctt gctggtgagg gacatatcca ccatcgccgt atcctgcccg
    10801 ggtgcggtgg cgcgcgacca cgcaccttcc acctgcacat ccgccgcata aagactggcg
    10861 ctcagcaaca gccctgccaa caccccaccg aaacggtaca aactgatcat gatattttcc
    10921 atcgataata ttttggagct gccgattata ccctcccccg gctcagggtt acgcgcacaa
    10981 aatgccaagg gagcgcacct ctgtcatgtc aactgggagg agacactgca tataaaggtc
    11041 atgtcgggat cgagactcgg ggccccctcg gcaaagccga ttattggaca aaagacccgc
    11101 tgcgtgaatg cggcattttg ccgcacccct gcaccgccaa caacccggta aaatcacgca
    11161 ccatgaacca catccccttc gccgcccaga ccggtcacag tcacttgcgt cgcctcgtat
    11221 ggctgcgcac gatcacggtg gcagcgcagt gcgccgcact cgtgctggta taccgcttcc
    11281 tgaaaatgga gctgcaatgg tcgcccatga tcaccgccat cgtggcgctc tccgcactga
    11341 atctggctac ctggctgcgt ctgaaatacc acagcaaacc ggtttccgcc gtggaactgt
    11401 tcacgcaact ttgcgccgat gtgctggcgc tgtcggtgct gctctacttc agcggcggct
    11461 cgaccaatcc cttcatctcg ctgtatctgc tgccgctggt catcgctgcg gccacgctgc
    11521 cgcacggcta cacctgggcc atgaccgccc tcaccaccgc ctgttacacc ctgctgatga
    11581 agtactacgt gccgctgcca atgcatcacg agatgaccgg caacatgtcc ggcatggaag
    11641 gcatggacca cgacatgagc gcaatgaacg tgagcgacct gccgcaggag agcatattca
    11701 acctgcatgt gttcggcatg tggctgggct tcgtcatcag cgcggtggtc atagcctact
    11761 tcgtggtgaa gatggcgcac gaggtgcgcg aacgcgatgc cgcgctggcc agagtgcgtg
    11821 aagaaacctt gcgcaacgaa cgcatcgtgg cactcggcac ccaggcggcg ggggccgcac
    11881 acgaactggg cacacctctt tctacactag ctgtggtgat cggcgaactg aaacgcgagc
    11941 cgtccatgct gcccgaatgg cgcgctgatc tggacatcct cgacggacag gtacaggcgt
    12001 gcaaacgcat cctcgacaaa ttgctcgccc atgcccagga tcacgaaacc gcacaaacgc
    12061 tcgaacaatt cctcgccgaa accctgagcg aatggcagct gttacgcccc accgcacatt
    12121 accgttatca ggcgcccagc gcgcactctg ccccgcatct gcacatcgac ccggcattgc
    12181 gcgccgcgtt gctcaatctg ctcaacaatg ccgccgatgc ctctccggat gagatcgata
    12241 tcgaagccca ttggaccgca gagaatttca tgctgagcat tcacgaccat ggcctggggc
    12301 tcacaccaga ggccatcgcc cgggcgggct cggcattttt taccaccaag gaagaggggc
    12361 gcggactcgg gcttttcctc gccaacgcca ccgtggaaag gatgggcggc aaggtacgcc
    12421 tgttcaaccg cgaggtcggc ggcgccacca ccgagatcat cctgcccttg cagacacgca
    12481 gatcatgagc accctcccca acgagcaggc ctcgctgctg ctggtagacg atgaccccac
    12541 cttttgccgg gtgctggctg gcgcactgga aaaacgcggc tacagtgtct gcatagccca
    12601 tagcgtggag caagccatcc cgctggcaga ggccaacccg ccggaattcg cggtagtgga
    12661 tctgaagatg agcggcgtgc cgggactgac gctggtcaag gccctgcacg aactcgatcc
    12721 gaacacgcgt atcgtgatgc tcaccggcta cgccagcatc gccaccgccg tcgaagccat
    12781 caaactcggt gcgacccaat atctggcgaa accagccaac gccgacgaaa tcgtcgcggc
    12841 cttcagtcat gttcccgacg acagcgcccc gctcaggacg caacccacgc tgatcggcaa
    12901 tctggaatgg gaacatatcc agcgcgtgct gaacgaacac gatggcaaca tctccgccac
    12961 cgctcgcgca ctcaatatgc atcgccgcac cctgcagcga aaactgggaa aacctccctc
    13021 acatcccgga taattagccc tcgcggattc cgccattctc tcgtatagtt accctttggc
    13081 ggcaatcagc cacagattta gatcaacagc aacacacgag aaacatccga tgaccgacga
    13141 cgatacccgt gcaagactca aacactccct tgccaatctt gaatccctgc ccgcgatgcc
    13201 cagcatcgcc cagaaattgc tcgcacttcc tctggataca gaagaaggcg aaatggagtt
    13261 gctcagtctg attgaacagg atccacagat ttccgcgaaa ctgatcggtc tggcgaactc
    13321 gcccatcatg ggggtgatgc gcaaggtatc gacggtacac gatgccgcca tgctgctcgg
    13381 tctgacccgc gtcaagtcgg tatccatcgg catcgccacg atgtccgact tttccaggct
    13441 tcctgcaagc cggaacttca aaccccagga tctgtggctg cactgcatga tcaccgccgt
    13501 ggtgatgcgc accatcgccg ccgccatgcc agcccgcatt cgcccgcccg aagatcaaat
    13561 ctatctggcc ggtttgctgc acgacatcgg gttcatggca ctgcaccacc tcgaccacga
    13621 gctgagcaat gagctacatc gccagctcca cctgcaaccc gaacgcccgg tactggaaat
    13681 cgaactcgaa acgctgggga tcacgcattg ctacatcggc tcgcagatcg cgcgtcactg
    13741 gaacctgccg aaagaaatcg gcgaggtgct gggataccac caccctccct atgtcgatga
    13801 aatgccggag cagaatctgc tggccagact ggtcagcgta gccgagaaac ttcagcctga
    13861 cttcggcatc tccgaacaca ccggagacga agttcacgca catgaatggg ccgaattggg
    13921 catcgagttg tcggcggtag aagagatcgg catccaggcc aacgaactcg cgatacaggc
    13981 aacgcaaatc gccatctagt ggcgagagca tcgccccgga tggcttcgga ctatttcgct
    14041 tgcacgtgcc tgctgatgcc ggtcagcgct tgaatgatgg cacgcaaggc ctgattgtat
    14101 tcgttccgga atatctcgcg ggcggcggcg cgttcgccgg cctttatctt ttcgatgatc
    14161 aacgtgccga tatgatgaaa ctgctgatga gcctcactta acagcacaaa atccggcaac
    14221 tcccggaagg gttccagctc gggtccggtg atccagcacc ccagctgaca aaaaccgccc
    14281 tgccccacca gtacactttc cagcggttcg cggacattcc cctcgatatg gtgtcccaga
    14341 cgcgccttcc acaggatgtg ggcctcagcc gcaccgagca gattaagatc gcgatacgat
    14401 acggattgcg tagcactcaa cccgaaaaat tcctttacct ctgccagcaa tgccatttca
    14461 acccgccctc cgttgcaatc acagcaggtt tataaccggg ccggggcgct ataacaatag
    14521 acctgaatat ataggcattc cggcctaatt ccgccggctc ccgattaaat tccgcgagaa
    14581 atatctgcct cgctatcacc gcatgacatg cacggactat aattgccgcc ccaatgaaaa
    14641 cctgctgacc atgagatctg ttctgctatt cgccggcctg ctgctatcct ccgccgtctt
    14701 ctcagcaccg gcgccaatac ccgtagcgcc gcaactggcg gcgaaagcct atctgctcta
    14761 cgactacacc agcaaccaga ttctggtgaa caagaacggc gatacgcgca tggagcctgc
    14821 ctcgctgacc aagctgatga ccgcctacct ggctttcgac gcgctcaagc acggcaccct
    14881 gtcatcggat cagaccctga cggttccggc caccgcgcta cgcgccaagg gcggcgaatc
    14941 gcgcatgctg ctcaaggcag gacaggaagt caccgtggat gagttattgc gcggactgat
    15001 cgtgcaatca ggcaatgacg ccgccatcac actggccgtg aacatcgcgg gcagcgaagc
    15061 gggtttcgtc gacatgatga acaaagaggc caagcgtctg gggatgaaca acacccactt
    15121 caccaacgcg atcgggctgc cggatgccca gcactacagc agcgcatccg acctggcgct
    15181 gctgaccgcc gccgtcgtgc acgactatcc ccagcatcag cagctgttcg cgctgcgcga
    15241 atacagcttc aacaatgtca cccaggccaa ccgcaatcgc ctgctgtgga tggatccttt
    15301 cgtcgacggc atgaagaccg ggcacaccga gactgcggga tactgcctga tcggctcggc
    15361 caagcgcaac gaccggcgtc tgatctcggt gctgctcggt gccgatacgg acaacctgcg
    15421 ctccgtcgag agccagaaac tgctgaactt cggcttccag aatttcgatg ccgtgcagtt
    15481 atacaagaag gatcagccag ccacccagct gcgcgtatgg aaaggaaccg agcgtcaggt
    15541 cgcagtgggg tttcgcaacg acctgttcct gaccattccc aaggggaagc tcgcccagct
    15601 caaggcatcg gtggaaacgc gccagcccat cctcgctccg atcagcagtg gccaacaact
    15661 cggcacactc aagctgtcca ttgccggcca accctatgcc gagtatccgc tgatcgcgct
    15721 ggacgccgta ccgctggcca atgtgctgtc gcgcggctgg gacagcatcc gcctgttctt
    15781 tcaataggag agcgacatga ccatctatct gaacggcgca ttcatgcccg tcgaagaagc
    15841 gaagatatcc gtgctggatc gcggcttcat cttcggcgat ggtgtgtacg aggtcattcc
    15901 ggtttattcc cgccgcgcct tccgcttgcg cgagcacctg aggcggctcc agcacagtct
    15961 ggacggcatc aagcttgcca atccgcatag cgaggaagac tggatcacca tcgtcgaaca
    16021 gatcatcgag cgcaacccgg gcgaagatca gtatctctac ctgcacatca cgcgcggcgc
    16081 ggcgaagcgc gatcatgcct tccccaaccc gcctgttgca ccgacggtgt tcgtgctgag
    16141 cagcctgctc gcacagccat ctgccgagca gctgagcagc ggcatcagct gcatcaccgc
    16201 aaccgacaac cgctggctgc gctgcgacat caaggccata tcgctgctgc ccaatgtgct
    16261 gctgcgtcag atggccgtgg atgcgggctg cgccgagacc gttctgatac gcgacgacag
    16321 cttcctgacc gaaggtgcgg ccagcaacat cttcgtggtc aagaacggcg cgctgctcgc
    16381 accacccaag gacaacctga tgctgcccgg catcacctac gatgtgatcc tcgaactcgc
    16441 tgccgccaac ggcatcccct gcgaagtgcg acgcgtgatg aaggcagaat tgttcagcgc
    16501 cgacgagttg ctgctcacct cctccaccaa agaggtgctg gcgatcaccc ggctcgacgg
    16561 acagagggtc ggcagcggca aaccgggagc gatgttcgcc cggctgcacc agctctatca
    16621 ggacttcaaa cacaatgtga tgcgcaaatg aacgataccg tcaaaaacga actcacccac
    16681 agcctgatcg aatacccctg cgactttccg ctcaagatat tcggccagca acaggcgggc
    16741 ttcgcacagg ccgtgctgga agtggtcacc aaacacgatc cgggatttct ggcatccagc
    16801 atggagatgc gctccagcaa gaacgcgcgc tacgtcagcc tgacctgcac catccgcgcc
    16861 acctcgcgcg aacaactcga tgcggtctat caggagctgt gcgaccaccc gatggtcgtg
    16921 atggtgctgt gatggcgagc ggagagttgg aagcggggag cgggaaacac cccgccacgc
    16981 ccgccatcca tgcgctcgga atggtcgact atcagccgac ctgggaggcg atgaaacgct
    17041 tcaccgccga acgcaccgaa gaaactcgcg acgagatctg gctggtccag caccctccca
    17101 cttacaccca gggtctggct ggcaaaccgg aacatctgct caatccgacc gcgattcccg
    17161 tggtcaagat cgatcgcggc ggccagatca cctatcacgg cccggggcag attcttgcct
    17221 acctgctgct ggatatgcgc cgctggaaga tcggagtgcg cgaactggtg cgactgatgg
    17281 aacaggcggt gatcgacctg ctcggcgagt acggcgtcgc cgcgcacagc cgcgaagaag
    17341 cgcccggcgt ctacgtcaac ggcgccaaaa tcgcttcgct gggattgaag atcaagaacg
    17401 gctattgcta ccacggtctc gcactgaacg tcgatatgga cctgtcgccg ttcctcaaca
    17461 tcaatccctg cggctacgcg ggcctgcgcg tcacacaggc atccgagctg ggcgtcgccg
    17521 ccccgatcaa cgagctacag gccgaactgg cacagaatct gatccacgga ctccagcagc
    17581 atctcgcccg caagccttag tcttccccgc cccagcgctg ttgcaggctg tgcgccaccc
    17641 ccagatgatc gaggatgcgc gccacgacga aatccaccat atcctgcacg ctctgcgggt
    17701 gatgatagaa acccggactg ggcggcagga tgaccgcacc ggcgtgcgac agccgcaaca
    17761 tattctccag atggatcgct gaaaacggtg tctcgcgcgg caccagcacg agcgggcgtc
    17821 tctccttgag catcacgtcg gcggcacggg caatcagatc atcgccgata ccgcaggcaa
    17881 tcttgcccag cgtcgccatg gtgcagggac agaccaccat cgcgtcaccc ggattggaac
    17941 cggacgccat gggcgcgtac cagtcgtctc gtccatatac ccgcagctcg ccgctgaatt
    18001 ctccgacgcg ttccgccaac agcttctccg catcctgagg ccggctgggc aacacgatat
    18061 ccatctcctg cttggcgacg atctgtgcgg cctgagaata aaccagctgc acgcgcacgc
    18121 cagctttaag caggcattcc agcaggcgca tgccataggg cagaccggac gctccggtca
    18181 aggcgagggt gatggttttc atgcggcaga ttccggtgaa cgttcggcgt cattatccat
    18241 gaatcgcgcc gcgatgagtc cgttcaccgt gccgaacacc agcgctgcgg tagcgaagat
    18301 cgggatcagg taggcgatgc ccgcgtgcgg aatcagccac aggtacacca ccgccatctg
    18361 cccggccaca tgggcgaagg cggcatagat gctatggctg acgacaccga accagcgcga
    18421 tggtagctgc cgcgcgaagg ccagcaccag cagactgcac agcgcgccgg acagactgag
    18481 aaagaatccg ggagccagaa agttgccgaa caacagactg cccgccagta cgcgcagcaa
    18541 cgatacccac acggcgaccc gccagccata gcgcgccagc acgatcagcg taacgatgtt
    18601 ggccagtccc ggcttcacgc ccggcagcgg cgacgggatc gcgctctcca gcacggtcag
    18661 gcccagtgcg actgccgcca tgcgcgcgac gtgatgatct gcagcggtgg cgttcagcag
    18721 gattgaggat tgaggatctt gtgaggatcg agttaaaagc ggcgcggcga tttctcctcg
    18781 ctccccgctc ctggcttcta gcttctgctt ttcagtaatt gagactgtca tatttcttcc
    18841 ggctgccggt caattccacg ctcacctcat tcggcaggca gatcgcaatc tccccggact
    18901 gcgccagcca gccttgccgc acgcagtatt ggcggggact gggatcggag ctgatgcgcg
    18961 ccttgtgctg ccgaatgctg acgatactca cgcccaacgg cccggggact tcgatctgct
    19021 gatcgcgcga caacggcact tcgcgaaata ttttcccgcc gctgcgaatg atggctttgt
    19081 cggcaaggtc gccgctccat aattcgcgca tcaacaatac gacgcaaaga ctgccgaaca
    19141 atagcgtcag ccagtcaccg ggcttgaggt gcggcagaat ggccgcacga acagcttcat
    19201 gcgagctcac ggtggcgcac cagcacctgc tgctgcccct cgaagtggcg tgccagcctt
    19261 tcggcaagat agaccgagcg atgctgcccg cccgtgcatc cgatggcgac ggtgaggtaa
    19321 ctgcggctgt ctgcgatgta acagggcagc cagttggcga cgaagtcccg gatatcggcg
    19381 tacatcttct gggcctgctc gctggcctcg aggaagtcag ccacggcggc atcgcgtccg
    19441 gtcagcggac gcagcctctc gtcgtaatgc ggattgggca ggcagcgcac atcgaacacc
    19501 agatcggcgt ccagcggaat gccgtgcttg aaaccgaacg actggaacaa caaggtcagc
    19561 cgcgcgcgat ccagcgggat gaaccgtttg atccaggcac gtagcgcatt ggcattcagg
    19621 tccgaggtgt cgatgtggtg accgatgccg ccgatgccgg ccagcagttc gcgttcgtga
    19681 gccacgcatt ccggcagcgt cttcaccccg tcgttgagcg ggtgcaggcg gcgcgtctcg
    19741 gagaagcgct tcaccagcgt atcggtgcgc gcatccagat acagcagatg cacggccagt
    19801 tccttctgca tgagttgcag catcagcgat ggcagcacct gcacgctgtg cgcattgcgc
    19861 acatcgatgc tcaccgcgat gttcctgtaa cccgtcggca gcaactgatc gatcagattc
    19921 ggcaacagct cggcaggcaa gttgtccacg caatagaagc cggcatcctc cagcgtcttg
    19981 agcgcgacgc tcttgccgga gccggacaga ccgctgatca gaatgagttg catctcgacc
    20041 ggatctaatc gttttgcagc aattgatcgt gccgcaggat gaactcctgc atgctgtcga
    20101 taccacgctg ctgcaacacg aaattgcgcg cggccgcctc gaccagcacc gacaggttgc
    20161 gtccggcgac caccggaatg ttcaccgtac tgacgtgcac tccgaggatc tcctgatagc
    20221 tggcattgat aggcagtcgc tcgacctgag acaggtcgcc gccgggcggg cggtgcagat
    20281 gcacgatcag cttcaggctt ttcttgcgtc gcaccgcggt ctcgccgaac atggtgcgaa
    20341 tgttcagcac ccccagtccg cgcacctcga ggaagtcgcg caacagctcg ggacagcggc
    20401 cttccagcgt gtccggcgcg atgcggtgca actcgacgat atcgtcggcg accagaccgt
    20461 tgccgcgcgt gatgagctcc agccccagct cgctcttgcc caccgcgctc tcgccggtga
    20521 tcatcacccc gacgccgagc acatcgagaa acacgccgtg acgcgtggtg gactcggcca
    20581 gttccttggc aagatagtgg cgtatcagcc acatcagatg cacgctgggc ttggatgagc
    20641 ggaacagcgg gatgtgcgtg cggttggcga aatccagcaa ttccgccggg atgtcgtgcg
    20701 cccccgccac gatcagacag gtcggactgc tctgctcgat ctggcgaaat gccgcttcga
    20761 gttcgctggc gctcaatccg cgcaggtaat tcagttcggt ctcgctcagc acctgcaccc
    20821 aattcggatg aatcagattg aggtggccga tcagtccctt gttggaggca ttgacctcgt
    20881 cggcgctgat gatgcggtcg ttgccatccg ccccggccac acggctgagt tccagccggg
    20941 cctgcttgtc ttcgaacagt tggcggacgt tgacttcact catagcgaac cattccagtc
    21001 gtagaacagt tgatggatgc tgggctcatc ttcgcaggcc agcaggcgat tgcgaaattg
    21061 cgagtcgctg aacatctgcg ccaactcacc gaggatctgc agatgctggt cggtagcccg
    21121 ctccggcacc agcagcacga agatcaggtt caccggctcg ccgtccggcg catcgaaggg
    21181 aataggatga ctggttttaa cgaaggccgc agtcgcatcg cgcagcttgg cgatacgtcc
    21241 gtgcggaatc gccacgccgt gccccaggcc ggtcgagccc agcttttcac gcgcgaacag
    21301 gctatcgaac acctgactgc gtgcgatttg ctgattgttc tcgaacagca gccctgcgcg
    21361 ctcgaacacc cgcttcttgc tggtggactc cgtatccagc aggatattgt cgggagccag
    21421 gattttactg atcaggttca tgggaatttt ggaattgaaa atgggcggta agaccgccca
    21481 ttgttgatta ctctgccgca gcaggtttgc cggaatcgtc atggcgacgt gccgccagtt
    21541 tttccttgtg cttcaacaca ttgcgatcga gcttgtccac cagcgtgtcg attgcggcgt
    21601 acagattttc atcttcgcat tccacgaaca catcgtggcc gctgatatgc acggtcgctt
    21661 cggctttctg tttcagtttc tccaccgaaa ggatgatgtt cacgtcgatc acattgtcga
    21721 aatgacgttt gatcttgccg aacttgccgt tcgcatattc gcggatggcg ggagtgattt
    21781 ccagatggcg tccggtgaga ttgatgttca tgatgcgctc ctttgtttta gagtgattta
    21841 cggagattca ccgggaggat atgtaacgct tcccggtact ttgctatggt acgccgggcg
    21901 actacgatgc cctgctgtgc caagatttct gacatacggc tgtcggtaag cggtgtgcgg
    21961 gtattttccg cactgaccag ttgcctgatc agttcgcgta tcgctgccga agaacaggtt
    22021 cccccgctct ccgtgaccag accgctgcca aagaaatgtt taaattcgta gatgccgcgc
    22081 ggcgtcagca tgaacttctg cgtggtgctg cgcgaaatgg tggactcgtg caactcgagc
    22141 tcctcggcga tctcgcgcag taccagcggg cgcataccga tctcgccgtg ctcgaagaac
    22201 ttctcctgac gctccacgat cgcctgtgcc acacgctgga tggtctcgaa acgctgctgc
    22261 agattcttga tcagccactt ggcctcctgc aagcgcgtcg ccagttgcga gccgttaccc
    22321 tcgtcgcgct gctggaggat atgggcatag acctgattga cgcgcagctt cggcatggcc
    22381 tcggcattca accgggcgcg ccatttgttc tggtgcttct cgacgatcac atccggcacg
    22441 acatagtccg cgacgcgatg gtcgaactcg gcaccaggtt taggattcat gccgacgatc
    22501 agatgctggg ccaagcgcaa ctcgtcgtcc gagcaatgca gtaatttttt aagttttgcg
    22561 aaatcgtgtg cggcgagtat gtccagatac tggctggcca gatggatcgc cagatcgcgc
    22621 tgcggcgtat cttccggcaa cgccttaagc tgcaatgtca gacactcggc cagattacgc
    22681 gcccccagcc ctggagcatc cagatgctgc aactgaacca gtgcggtctc aagatcatcc
    22741 agtgtgatat ccagctccgc tggcaacagc tcggccagct ccaccagatc ctgggcgaga
    22801 tagccgttct cgtccaccgc ctcgatcagc aggccgacca cgatgcggtc cctgtcctcc
    22861 agcgagccgg tggccagttg ctcatgcagg tgttcgcgca gactgatctg ttcggcggct
    22921 tgctcgggac gcccctcgtc ctcgtcgtcg gcgctgcttc cccgttcgcg cccctcccag
    22981 tctgcagcac tgagcgcgaa atcctccgcg cccaaccgct cttcgcctga atcttcggag
    23041 gtgcctgctt caggctggtc actgatggcc tcgctacgca cgtcggaaac aggcgacatg
    23101 gcgggcacat aatcgtcggc caattccagc atggggttct cgtccagcat gcgctcgatc
    23161 tcctgatgca catcctgcgt ggaaagctgc agcagccgga tagcctgctg caactgcggg
    23221 gtaagcgtca gctgctggga agttctgagt tggagagcgt gtttcattgc ctgttctggt
    23281 atccgggagc cattctgcgt tacagcttga aatgctcacc cagatatact tttctcacgc
    23341 cctcattata gatgatttca tccgggctgc cctccgccat cacggctccg gcattgatga
    23401 tgtaggcacg atcgcaaatc cccagtgttt cccggacgtt gtgatcggta atcaacacac
    23461 cgatgccgcg ctccttgagg aaatggatga tcttctggat atccagcacg gcgatcggat
    23521 ccactccggc aaagggctcg tcgagcagaa tgaagcgggg gtccgttgcc agcgcccgcg
    23581 cgatctcgac gcgtcgtcgc tcgccgcccg acaggctgac agcctgattg tcgcgtatat
    23641 ggtcgatatg caggtcttcc agcaactcgt ccagccgatt ctgcaaggcc tcatcggcca
    23701 gcccctgtaa ctccagcacg gcctgaatgt tttgcgccac gctcagacgg cggaagatcg
    23761 acgcctcctg cggcagataa cccagcccga ggcgcgcgcg gcgatggatg ggcagatggg
    23821 tgatgttctg gtcgtcgatg aatatctcgc cgccatcggc ggcaaccagc cccacgatca
    23881 tatagaagga agtcgtctta cccgccccgt tcggccccag cagaccgacc acctcgccgc
    23941 cgcgcaccgt cagcgacgca tcatgcacca cggtgcgtga accatatttt ttcttcagtc
    24001 cgactgcacg caattcgctc attccgcctc cgcaggtgca agggtatcac tgggcgcgat
    24061 aggcagcgcc tcgggcacgg acgaagccgc accctctctg ggtttgggct gcaaaatcgc
    24121 tctcacccgc ttcggcggcc cgccggcgac agcgccgcta ttgacccgga acacctcggt
    24181 cttctggcta taggtgatgt gctcaccgcg aacttcgtcc agtttgcgca ccaccctcgc
    24241 cctcacgtaa aaatccacgg tttcggcccg cgtgtcgtgc tcgatacgct cgccatagcc
    24301 ttccacatat tcatccagcc cctcccgctt ctgccggaag ctggccgtct gcccgtaggc
    24361 cgtggcatgc tgaaacccct ccgcatcctc ggtcaccacg atcttgtcgc cgcgtatcag
    24421 catcgtcccc tgggtcatct gtacgttacc cgtgaaggtg tccaccttct gcgcatcctc
    24481 caccagcacc tgatcggcct cgatgtgcac cggcttgtcg cgatcggccc gttccgccag
    24541 gcagggctgg gatagcagca gcccgagcaa caacagtcta gcgtggctca tggacactcc
    24601 tgacctgaga cagcaatttc aaggttcgtg ctttgttatc catttccagt cccaccgcgt
    24661 gaacgacatt acgctcatca aaaatagtga ccgcatgatc cgtgtccgcc cagtcccgat
    24721 ccggaacgac acgcagataa tcggtctgca gggtcagctc gccgcgcgtc gcactcgcct
    24781 cgcgcagcac ctcgacctcg tcatgcagaa acacctcgtt gccactgccg gagacgatgc
    24841 cgcgctgcgc aaccagatga accgtcggaa gcgtcgcgga aagcgaagcc atacgaggtg
    24901 cctccagcgc agagctatcg tcatccggat aatgctgcat cttcttggcc gacatgatga
    24961 agcggggcac cccctgatcg ttcatcttga ccgatgaaaa attttccatg atggcatcgg
    25021 gatcgtggcg catcccgcca ccggccgtct ccggctccag atgcgtctgt tgatccagcc
    25081 agtaggtcac gcccagaatt ccgagcagcg gcaagagcgg cagccagtaa cgggcgcgcg
    25141 agtagaaggt catttcagat aaggtgcaag ctgggcgtcc agcgtaccct gtgccgacat
    25201 gatcaattcg caggcttcgc gcaccgcacc atggccgccg ctgcgctggg tgacataatc
    25261 ggaatggttg cgcaccacct gcggcgattc cggcacactg atcgacaggc cgacgtgccg
    25321 catcacacac aggtccacca catcgtcgcc catataggcc gccgcatcgc gcgacaactt
    25381 gagcctgttg agcagatcga ccatcgcatc cagcttgtgc tccaccccct gatacacatg
    25441 ctcgataccc agattctgcg cgcgcaattc gacgcaacgc gaagtgcgcc cggtgatgat
    25501 ggcgatctcc accccgctgg cacgcaacat cttgatgcca tgcccgtcca gcgaattgaa
    25561 gcgtttcatc tcgtcgccgg agtcggtcag gaacaacccg ccgtcggtca tcaccccgtc
    25621 gacatcgaag gtcatcaaac gtatcaattt tgcgcgactc aacagcatgg attccccccc
    25681 gttattttca gctggaccta gatcaccttg gcatgcaaca ggtcgtgcat attcagcgcg
    25741 ccgaccaact tgtgctgttc atccaccacc aacatctggc tgatgttgta ctgctccatc
    25801 atctgcacgg cctcgacggc cagcgcatca ggcccgatac agcgcggctt ggcagacatc
    25861 accgagcgca ccggggtgct gccgaaatcc agtttctttt ccagcgtgcg gcgcagatcg
    25921 ccgtcggtat agatgcccag cacatgctgc ccggcgtcca cgatagcggt catgcccacg
    25981 cccttccggg agatctccag caacgcctcg cccagcatcg ccgcctcgcc caccatcggc
    26041 aggcgctcac cgctgcgcat gatgtcgcgc acatgggtga gcaggcgccg tcccagactg
    26101 ccgccgggat gcgagcgcgc aaagtcctcg gcaccgaagc ccttggcgtc cagcagcgcc
    26161 accgccagcg catctcccag cgccagtgcc gccgtggtgc tggctgtcgg cgcaagcccc
    26221 atcgggcagg cctctttgtc cactgccgca ttgagatgca cgtccgccgc catagccaga
    26281 ctggaatccg gattgccggt catgctgatc agcttcgccc cctggcgctt gatgaccggc
    26341 acgatggtca tcagctcctg gctctcgccc gaataagaca gcgcgatgat gacgtcctcc
    26401 tcggtgatca tgccgaggtc gccgtgactg gcctcgcccg gatgcacgaa gtaggccggc
    26461 gtaccggtgc tcgacatggt cgcggcgatc ttgcgcgcga tgtgtccgga cttgcccatg
    26521 ccgctgacga tgacacgccc ctcgcaacgc aggatgacat ccagcgcacg caaaaaggtc
    26581 tcgtcgagac gggtgaccag cgactgtatg gcggcggctt cgatactcag cacttcacgc
    26641 gcaagatcga gtgcgcgcgg cgcagcggaa agggatttat tcatggcgcg cattatagca
    26701 agggtggtgg aatcaaggca cttttgccac aaagaatgca cgccctcccg cagcacatca
    26761 ggaaaatttt gcaaacgacc cgacttacgg catcatcctg agaaagtcga ctcactcgaa
    26821 gcaaatggaa agcacactct ctctcgtcct gatcctgctc gccaccgccg tcatggtggt
    26881 ggtgctgtgc cgcatcctgc atctgccggt gatgctgggc tatatcgcgg tcggcatcct
    26941 gatcggcccg catgcgctgg cgtggatacc cgacgcgatg ggaacgcagc atctggccga
    27001 gttcggcgta gtgttcctga tgttcagcat cggactggag ttcagcctga cgcggctatt
    27061 ggcgatgaaa cgcacggtgt tcggcctggg tggcgcacag gtgctcgtca ccatcctgct
    27121 gacgatggcg atcgcggcat tgttcaaact ggactggcgc accggtctgg cgctgggcgg
    27181 cgtgctggcg atgtcgtcca ccgcgatcgt cagcaagatg ctggtggagc gcgccgagct
    27241 gaatctgccc tacggccagc agatgatggg catcctgctg tttcaggatc tcgccgtcgt
    27301 accgctgctg atcatcatcc cggcgctggc ctccaatccc gacgacctgc ccgccacact
    27361 ggctttcgcc accctcaagg ccatcggcgt gctgctggtg ctgctggtat tcggacagcg
    27421 catcatgcgc tcctggttcc atgtcgtggc gcggcagaaa tcctccgagc tgttcatgct
    27481 caacgtgctg ctgatcacgc tggggctggc cttcttcacc gaactggccg gactctcgct
    27541 ggcactgggc gccttcgtcg cgggcatgct gatctcggag accgaatacc gctaccaggt
    27601 ggaggaagac atcaagccgt tccgcgacgt gctgctcggc ctgttcttcg tcaccatcgg
    27661 catgaagctc aatctgggcg cggtcgttgc cgacttcggc tggatactgc tgacgatgac
    27721 gggactgatc ctgctcaaag cgctggtcgt ggcgctgctg gcgcgcacat tcgaaggcga
    27781 ctggggcgtg gcgctgcgca gcgcgatcgg cctggcgcag gcgggcgaat tcggcttcgt
    27841 gttgctgacg ctggcggatg cccacctgct caacgatgcg accatgcaga tcgtgctggc
    27901 ggcgatgctg ctgtccatgc tcgcggcacc gtttttgatc cagcacggcg aggccatcgc
    27961 acggcgcttc tccgcatccg aatggaccaa ccgcgcgatg cagatgcacc agatcgccgt
    28021 gcagagcatg ggccgcagcg cccatatcat cgtctgcggc tatggccgca gcggcggcgc
    28081 actggccagc ttcctgaaag gcgagaacct gcatttcatc gccctcgatc tggattcgcg
    28141 acgcgtacgc caggcggtca ccgatggcga aagcgtggtg tatggcgatg cggcaaaacg
    28201 cgaagtgctg atggccgccg gcctgatgcg cgccaaagca ctggtgatca cctacagcga
    28261 caaacactcc gcgctggcga tcctgagaca caccaaagaa ctgcgcccgg acatgccggt
    28321 gatcgtgcgc acccatgacg actcccatgt ggaagaactg aaagcggccg gtgcggcaga
    28381 ggtcgtcgcc gaggtactgg aaggcagcgt gatgctcgcc tcgcaggccc tgctgatggc
    28441 gggcatcccg ctcaaccgcg tgatccgccg tgtgcaggaa agccgcgcac gccgctacgc
    28501 atcgttcagc ggctatctgc gcaccgtccg cgatgatgtc ggcgaaatca ccgaaagcca
    28561 gcaatcgcgc ttcctcagcg tgctgctcaa ggaagactcg actgccgtgg gcaagcaact
    28621 gggcagcatc ggtctggatg cactgaatgt cgaagtcaac gcggtgcgcc gccacaacct
    28681 gcgcggctca caacccagcc cggacatggt catgcaggcc ggcgacgtgc tggtgctgtt
    28741 cggtttgctg gaagcgttgc aactggccga ggtcaagctg catcagggct aagcgcagca
    28801 atcagcagca gcttctccgc ccgcgtcagc cgcttgatcg ccagctcgcg cttcgaggcg
    28861 gcagaccggt cgtcgcaggg ttccacatac accagcgcta ccggcccgcg cgcccgcgtg
    28921 tacttcgccg ccgtcccggc attgtgcgcc gcgaggcgct tggatagatc gttggtgatg
    28981 ccgcaataga gggtgtcatc ggcgcagcgc agcaggtagc acagccagtt gcctttaagc
    29041 ggcgcggtcg tcggagcggt ttcgtttttc acgggcggca ttttacgatg ttgcgggatg
    29101 ctcgcccgga ctaagttgcg gttcggcatt tccgccgagc gggcatacga aaaaataata
    29161 tttcgcaaac ccagcgtcaa tggtcggcca cggcgggatg catccattcg acttttcccc
    29221 gcagtagcgg tagcatcgcc gccctttttt atcggcccgg cttcaggcca tctttcgccc
    29281 atgtctgttg aaactcccgc ccgcgcctgc ggcatcgatt tcggtacctc caattccacg
    29341 gcgggctggc tgcgtcccgg ccagtcgccc ttgctggccc ttgaagacgg caagatcact
    29401 ttgccttcgg taatcttttt caatgccgac gaagacaccg tcagcgtcgg ccgggccgcg
    29461 ctcaacgaat atatggaagg ctacgaaggc cgcctgatgc gcgccttgaa gagtctcctg
    29521 ggcagcagcc tgatggaagg ccagaccgag gttcaagggc ggccgcttcc ctataaggca
    29581 ttgctggcgc aattcatc
//
LOCUS       DASUIV010000061         9734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_16535, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000061 DASUIV010000000
VERSION     DASUIV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16535"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFW59_02175"
     CDS             complement(<1..536)
                     /locus_tag="VFW59_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5363049.1"
                     /translation="MTILLWIVIASIAGGVLSVLGAALFAFNSHMQRYLGAMVSYAIG
                     ALLGAVFLDILPEAITLSTNVAALSATVLFGIMLFFILEKLVLWRHCHHEHCEAHESP
                     ADAHSHDHGRTGMMITVGDTFHNFVDGVIIAAAFLTDVHLGMVTALAIIAHEIPQEVG
                     DFVILLHSGYSKLRAFQL"
     gene            complement(602..1336)
                     /gene="pgeF"
                     /locus_tag="VFW59_02180"
     CDS             complement(602..1336)
                     /gene="pgeF"
                     /locus_tag="VFW59_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX5363050.1"
                     /translation="MRLLEHCIVPDWPAPANVKTLQTTRHGGFSAAPYDSLNLGAHVG
                     DAPLVVARNRMLLNSLLPSEPVWLEQVHGTAVANADRAGCLIQADACIARQRASVCVV
                     MTADCLPVLLCDEQGTVVGAAHAGWKGLAAGVIDATVQAMAVEPGDLMAWLGPAIGPQ
                     AFEVGGEVRDAFIARHPAAAAAFVSAQPGKWFADIYALARLRLNALGVTRIYGGDRCT
                     YSEREQFYSYRRDGVTGRMGSFIWLV"
     gene            complement(1323..2225)
                     /gene="rluD"
                     /locus_tag="VFW59_02185"
     CDS             complement(1323..2225)
                     /gene="rluD"
                     /locus_tag="VFW59_02185"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEX5363051.1"
                     /translation="MRFDQVLAKLLPEYSRSRLQEWIAAGGATLDGAPAIAKQKVWGG
                     EAIAVSPQLNPAEQPYLAEDIALDIVYEDDALLVINKPVGLVVHPGSGNWEGTLLNAL
                     LHHAPQLEGVPRAGIVHRLDKDTSGLLVVAKTLTAQTNLVRQLQARSVKREYLALAWG
                     ELRRAGRVDEPIGRHPTQRVKMAVVEDGKPAVTHYQIEERFPSCTLLRCRLETGRTHQ
                     IRVHLAFEGHPLVGDSVYMKGVQKCAPELRELLHGFPRQALHATRLALEHPETGEMME
                     WEAPLPEDMQQLLQQLRAASHASA"
     gene            2308..3102
                     /locus_tag="VFW59_02190"
     CDS             2308..3102
                     /locus_tag="VFW59_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5363052.1"
                     /translation="MRHSLAVFLLLTLTACSLFEPLPTGTEPTALLSAEELYKQGMDE
                     LNDSNYSKAAELFEKLQSRYPYGHYAQQALLEAAYAYYRHEEPDAAISAADRFIKQYP
                     NNPNVDYAYYLKGLVYFNGEIGVLKALGGQDPTERDPKAAQDAFATFKNLVTRFPDSK
                     YAPDARLRMQYLTNSLAQYELHVARYYLRRGAYIAAVNRAQNVVTEYPNSPATRDALE
                     IMAQGYDAMGMTDLRDDVRRVLAKNSGPAPAADIVIVKKSWWQFWK"
     gene            3099..3644
                     /locus_tag="VFW59_02195"
     CDS             3099..3644
                     /locus_tag="VFW59_02195"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5363053.1"
                     /translation="MNFCPSCGAAVELRCPDDDNRPRHICTSCNTIHYQNPNLVIGSI
                     PEWEDKILLCRRAIEPRHGLWTLPGGFMENGESTTEAAIRETLEEAEARIEIGDLYSM
                     YSLPYLNQVHLLFRARLLDLNFGPGPESLEVRLFAEDEIPWDEIAFRPIKYSLEHYFS
                     DRRKGHFGFHIGALDRPAPIV"
     gene            3780..4631
                     /locus_tag="VFW59_02200"
     CDS             3780..4631
                     /locus_tag="VFW59_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX5363054.1"
                     /translation="MVSLAVALATVALTSGCATHANNPADPLESFNRGMYQVNDTLDK
                     AIAKPVAQGYDAVMPLPGKIMVSNFFSNLDDILVTANDVLQLKLRQGFSDGMRVVVNS
                     SIGVGGLFDFASMRLEKHNEDFGQTLGYWGVGPGPYIVLPILGPSTLRDGAGLYVDGQ
                     PSRLRRVSHIPTRNQLYATKLISRRAELLKQEKVLDEAVIDRYAYIRDAYLLRRQSLV
                     YDGNPPRPEYDEYEEEDYIEPAKPAAPSSQQKRVPAVHSGLSVEIQQPVLIPLEHRAS
                     AETTQYR"
     gene            complement(4720..5601)
                     /gene="htpX"
                     /locus_tag="VFW59_02205"
     CDS             complement(4720..5601)
                     /gene="htpX"
                     /locus_tag="VFW59_02205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEX5363055.1"
                     /translation="MKRIFLFIMTNLAVLFVINITLRLLGVDRWVNETGAINFGSLLV
                     LSAVIGFSGSIVSLLMSKWSAKRMVGAQVIENPSDPTERWLVETVRRQAGAAGIGMPE
                     VAIYDAPDVNAFATGWNRNAALVAVSTGLLHNMSRDEIEAVLAHEISHVANGDMVTLA
                     LIQGVVNTFVIFLAKLFGYFVDRVLLKNDGRDGPGIGAFVAEIAAQLVLGVLASVIVM
                     WFSRQREYRADAGGASLAGREKMIAALQRLKMNHEQASLPEQMAASGISGGKGMMRLF
                     MTHPPLDERIEALRSAR"
     gene            5880..6236
                     /locus_tag="VFW59_02210"
     CDS             5880..6236
                     /locus_tag="VFW59_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5363056.1"
                     /translation="MKYLAKYILLTICLLSTSINAIAAPRTLLRSASRIEFAIKEMGV
                     PATGEFSRVEARLNMDPARMEASGAELRIDTGSLITGNNECNLGDIVNNDILAKTLLT
                     LAANEPRAPPSQTKNQ"
     gene            6233..6937
                     /locus_tag="VFW59_02215"
     CDS             6233..6937
                     /locus_tag="VFW59_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5363057.1"
                     /translation="MTSHPTITTNQTTILRAENLALLFDDGQTRALNGLNVEINEGEF
                     VAIVGPSGCGKSSLLNLIGTLDIPTSGEIYFRSRPYSAIRDLSLFRRQNIGFIFQSFY
                     LLPTLSALDNVLVPTIGCPGAAQEHEERARSLLSRLGLDKRLDHFPGKLSGGERQRVA
                     IARALINDPDIILADEPTGSLDSANATQVLELIDGVRKEKELTVVMVTHDRSVSARAD
                     RIIRMRDGQIESEAQP"
     gene            6934..8061
                     /locus_tag="VFW59_02220"
     CDS             6934..8061
                     /locus_tag="VFW59_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5363058.1"
                     /translation="MNLFRFALDNLYRRPARTGLTVSAISLGIAAVVALTSIAWGFEA
                     SWQKANDARGTDLIVTRIASENTMPSPFAAEQVGKTLLTFPHVKEVVGLLSEMLTISE
                     DAPPVFVFGWAHGSYLWDHLKLRDGRWANSDAEAVVVVGSLASELLHKKIGDALEIEG
                     RAFQVVGIFESSAMIENGAVLMTLTQAQQITDKPGKVNILNIKLDSNTSETDIEAIKA
                     QVRKDLPGFIAITSGELVQKNAVVRISKAMSNATVLIASLVGALVVFNTMLMSINERT
                     REIGILLALGWQRRTIIKLIISESMILTLLGGTFGVLLGVAITWGLEHLELMRGKIDA
                     VFSTPFLFSVLGLSVLLGICGGLYPAIKAARLLPSNALRQE"
     gene            8058..8825
                     /locus_tag="VFW59_02225"
     CDS             8058..8825
                     /locus_tag="VFW59_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363059.1"
                     /translation="MKHIALRLAATLLLAAFAQPGAAADAAALAEAMQQARYSDGFTV
                     RMNIASIQPNGRRAMPFKLAVIGQIDAKRQRLLIKGISPDMVRNRFVVAERNADGRIR
                     AIGYTEPSAADIGKVDPTAPLFDSGLVLWDMLAPWWEWPRQNMGETEQIAGRSCTLVR
                     SQTDENFSPIREVISCVDEKARLSLRTQLFDRRHTLIRTISVKQTMRKDSGALAAKKL
                     TITDHAVTEVEVYSGDEHYQVTADTFAQFDAYRAAEK"
     gene            8834..9619
                     /locus_tag="VFW59_02230"
     CDS             8834..9619
                     /locus_tag="VFW59_02230"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5363060.1"
                     /translation="MQHLEHLTTSEDLSGTWCNANAVTSSIMEAVSLVTPVLEKFFIR
                     TVNEGLSERTSLALRERCLTFIREEADHSRVHKKFNTSVLHYLGQTPPGLARLEALLD
                     GTRNRLSLPSRLLLAAALEHFTAVLSKVYLAQEGRLDFQSAFAQALFAHHAREELAHR
                     SVVFDLWLTLGTSGRFKRSLTVFTILLVGITYIAVAVPWILHRKTGKLSATLSSLLSF
                     AVKNRSNIPSYSPLAELFSFVRRSYHPDHLVDDRATPVGQHRA"
BASE COUNT         2074 a         3193 c         2668 g         1799 t
ORIGIN      
        1 ttcagttgaa aagccctgag cttgctgtaa cccgaatgca gcaggatgac gaaatcgcct
       61 acctcctgcg gaatctcgtg cgcgatgatc gccagcgcgg tgaccatgcc cagatgcacg
      121 tcagtcagga aggccgccgc gatgatgacg ccgtccacga agttgtggaa ggtatcgccc
      181 accgtgatca tcatgccggt acggccgtga tcgtggctgt gtgcatccgc aggcgactca
      241 tgcgcctcgc aatgctcgtg gtgacagtgc cgccacagca ccagtttttc cagaatgaag
      301 aacagcatga tgccgaacag caccgttgcg ctcagcgcgg ccacattggt ggagagtgtg
      361 atcgcctcgg gcaggatgtc gaggaacacc gcacccaaca gtgcgccgat ggcatagctc
      421 accatcgcgc cgagatagcg ctgcatgtgg gagttaaacg caaacagcgc cgcacccagc
      481 acgctcaaca cgccgccggc gatgctggcg atgacgatcc aaagcagaat ggtcatggaa
      541 ttcattcgat caatcagggg cgcggattat agccgtttgg cgcacaaatc gctgacgcgc
      601 ctcacaccag ccagatgaag ctgcccatgc ggccggtcac cccatcgcgc cgataagagt
      661 aaaactgctc acgctcgctg taggtgcagc ggtcaccccc gtagattcgc gtgacgccga
      721 gcgcattcag gcgcagccgt gccagcgcgt agatgtcggc gaaccactta cccggttgag
      781 cggaaacgaa agccgcagct gcggcgggat gccgggcgat gaatgcgtca cgcacctcgc
      841 ccccaacctc aaaggcctgc ggcccgatgg cggggcccag ccaggccatc agatcgcccg
      901 gctcgaccgc catcgcctgc acagtcgcgt cgatcactcc cgccgccaat cccttccatc
      961 cggcgtgcgc cgcaccgacc accgtaccct gctcatcgca caacaacacg ggcagacagt
     1021 cggccgtcat caccacgcac accgatgctc gctggcgcgc aatacaggca tcggcctgaa
     1081 tcaggcaacc cgccctgtcc gcattcgcca ccgccgtgcc gtgcacctgc tccagccaca
     1141 ccggttcgct gggcagcaga ctattgagca gcattcggtt acgcgccacg accagcggcg
     1201 catcgccgac atgggcgcca agattcaggc tgtcgtaggg tgcggcgctg aaaccgccgt
     1261 gacgcgtggt ctgcagcgtc ttgacgttgg cgggcgcagg ccagtcagga acgatgcaat
     1321 gttcaagcag acgcatggct tgctgcgcgt agctgttgca acagttgctg catatcttca
     1381 ggcagcgggg cctcccattc catcatctcg ccagtctccg ggtgctccag tgccagacgg
     1441 gtggcatgca gggcctggcg ggggaaaccg tgcagcagtt cgcgaagttc aggggcacat
     1501 ttctgcacgc ccttcatgta gacgctgtcg ccgaccagcg gatgcccttc aaaggcgaga
     1561 tgcacacgga tctgatgggt gcgcccggtc tccaggcgac agcgcagcaa cgtgcagctc
     1621 ggaaagcgct cttcgatctg gtaatgcgtg acggcaggct tgccgtcctc caccaccgcc
     1681 atcttgacgc gctgggtggg gtgacgaccg atcggctcgt ccacacgacc cgcgcgccgc
     1741 aactcgcccc aggccagcgc aaggtattcg cgtttgacgc tgcgcgcctg caactggcgc
     1801 accagattag tctgcgcggt cagggtcttg gccaccacca gcagaccgct ggtgtccttg
     1861 tccagccgat gcacgatacc ggcgcgcggc acgccttcca gctgcggtgc atgatgcagc
     1921 aaggcgttga gcagcgtgcc ttcccagttg ccgctgcccg gatgtaccac cagcccgacg
     1981 ggcttgttga tcaccagcaa cgcgtcgtct tcgtagacaa tatccagtgc gatatcttcg
     2041 gccagatagg gctgctcggc aggattcaat tgcggcgata ccgcgatggc ttcgccgccc
     2101 cacaccttct gtttggcgat ggcaggcgcc ccatcgagcg tggcgccccc cgctgcgatc
     2161 cattcctgca ggcggctgcg cgaatattcc ggcaacagct tggcgagtac ctgatcgaaa
     2221 cgcatgccgc cacaatcgtt cggcacgaca aaatggaggg cgggtgcgtt taagttataa
     2281 tcgtgcaaat ttttttcgga ttcagtcatg cgccatagtt tagccgtatt cctgctgtta
     2341 acgttaactg cttgcagcct gttcgagccg ttgccgacag gaacggagcc aacagccctg
     2401 ttatccgccg aagaacttta caagcaaggc atggacgagc tgaacgacag caactacagc
     2461 aaggctgccg agttgttcga aaagctgcag tcgcgctacc cctatggcca ctacgcacag
     2521 caggcgctgc tggaagcggc ctacgcctac taccggcacg aagagcctga tgcagcgatt
     2581 tccgccgcag accgcttcat caagcaatac cccaacaacc ccaatgtcga ctatgcctat
     2641 tacctgaagg gcctggtcta tttcaacggt gagatcggcg tgctcaaggc gctgggcggg
     2701 caagacccca ccgagcgcga tccgaaagca gcacaggatg cattcgccac gttcaagaat
     2761 ctggtgacgc gtttccccga cagcaaatat gcgcccgatg cccgcttgcg catgcagtac
     2821 ctgacgaact cgttagccca gtacgaactg cacgtcgccc gctattacct gcgccgtggc
     2881 gcctacatcg ccgcagtcaa ccgcgcccag aatgttgtta ccgaatatcc gaacagcccc
     2941 gccacgcgcg atgcgctgga gatcatggcg cagggatacg atgcgatggg catgaccgac
     3001 ttgcgcgacg acgtccgacg cgtgctggcg aaaaacagcg gcccggcacc tgccgccgat
     3061 atcgtcatcg tgaaaaaatc ctggtggcaa ttctggaaat aaatttctgc ccatcctgcg
     3121 gcgcggcggt cgagctgcgc tgcccggacg acgacaatcg tccgcgccac atctgcacct
     3181 cgtgcaacac catccattac cagaacccca atctggtgat cggctccatt ccggaatggg
     3241 aagacaagat cctgctgtgt cgccgcgcga tcgaaccgcg ccacggctta tggaccctgc
     3301 ccggggggtt catggaaaac ggcgagtcga ccaccgaagc cgccatccgt gaaacgctgg
     3361 aagaagccga ggcccgtatc gagatcggcg acctgtactc gatgtacagc ctgccttacc
     3421 tcaatcaggt gcatctgcta ttccgcgccc ggctgcttga tcttaacttc ggaccggggc
     3481 cagaaagtct cgaagtcaga ctcttcgccg aagatgaaat cccctgggac gaaatcgcct
     3541 tccgcccgat caaatacagc ctggaacact acttttccga tcgtcgcaaa gggcatttcg
     3601 gcttccacat cggcgcattg gatcgccccg cccccattgt ctgaaggaaa ctccgggcag
     3661 ttttgccgaa tcccgctccc ctcgctataa tcagcgtacc catttgcacc gacaaaaaaa
     3721 tgaactcatc ccacgtcatg ccggttaaac aaccagccat tcgatcaggc cgccacaaga
     3781 tggtatcgct cgctgtcgcg cttgccaccg tcgcgctgac cagcggctgc gcaacccatg
     3841 caaacaaccc agcggatccg ctggagtcgt tcaatcgcgg catgtatcag gtcaatgaca
     3901 cgctggacaa ggccatcgcc aaacccgtcg cacagggcta cgacgccgtc atgccgttac
     3961 ccggcaaaat catggtgagt aacttcttct ccaatctgga cgacatcctc gtcaccgcca
     4021 acgatgtgct gcagctgaaa ctgcgtcagg gcttctcaga tgggatgcgc gtcgtggtca
     4081 actccagtat cggcgtcggc ggactgttcg atttcgcctc gatgcgactg gaaaaacaca
     4141 atgaggattt cggtcagacc ctgggctact ggggcgtcgg ccccggcccc tatatcgtcc
     4201 tgcccatcct cggcccaagc accctgcgcg acggtgcggg gctatacgta gacggtcaac
     4261 ccagcaggct gcgccgcgtc agccacatac cgacgcgcaa ccaactgtat gcgaccaagc
     4321 tcatcagccg acgtgccgag ttgctcaaac aggaaaaggt gctggatgag gcggtgatcg
     4381 accgctacgc ctacatccga gacgcctatc tgctgcgccg ccagagtctg gtttacgacg
     4441 gcaatccgcc gcgcccggag tacgacgaat acgaagaaga ggactacatt gaacccgcaa
     4501 agccagccgc accgtcttct caacagaaac gcgtgcctgc tgtccattcc ggcctttctg
     4561 tcgagataca gcagcccgtg ttgattccac tcgaacatcg ggccagtgcc gaaacgactc
     4621 aatatcgata gtctgcgaca gacggaagcc aacggcatcc gctaacccgc ccaacaaaaa
     4681 ggcccgcgac tgcgggcctt tttcattcca acgaacgcct taacgggctg aacgcaacgc
     4741 ctcgatgcgc tcgtccagcg gcgggtgcgt catgaacaag cgcatcatgc ccttgccacc
     4801 ggagataccc gaagctgcca tctgctccgg caagcttgcc tgctcgtggt tcatcttcag
     4861 gcgctgtaac gccgcgatca tcttctcgcg cccggccaga ctggcgccgc ccgcatcggc
     4921 acggtactcg cgctgacgcg agaaccacat cacgatcacg ctagccagca cacccaacac
     4981 caattgcgcg gcgatctcgg ccacaaaagc gccgatgccg gggccatctc tcccatcatt
     5041 cttcaacaac acgcgatcaa cgaaatagcc gaacagcttg gcaaggaaga tcacaaaggt
     5101 attgaccacg ccctgaatca gcgccagcgt caccatgtcg ccattggcca catgactgat
     5161 ttcatgcgcc agcactgcct cgatctcatc gcggctcatg ttgtgcagca gccctgtgct
     5221 cacggccacc agcgcggcat tgcgattcca gccggtggca aaggcgttca catccggcgc
     5281 atcataaatc gccacctccg gcataccgat accggcagca ccagcctgac gccgcaccgt
     5341 ctccaccagc cagcgctcgg ttggatcgct cggattctcg atgacctgcg caccgaccat
     5401 gcgtttggcc gaccacttcg acatcagcag cgaaacgata gagccggaga aaccgatcac
     5461 cgccgacaac accaacaggg aaccgaagtt tatcgcgcca gtctcgttca cccaacgatc
     5521 cactccaagc aggcgcagcg tgatattgat cacaaacagc accgcaaggt tggtcataat
     5581 gaacaggaaa atacgtttca tacaatctcc atcaaaaatt ttgctctcgg tttaacagca
     5641 tggaacttgg taccagtccg ccagtctcat ctcgcctgca atgagcgcag ggacatacta
     5701 gcgacgtctg acatggggga tgcgtaaaag tttttcaagt ccggacattt atttgatgcc
     5761 acagcacgaa aaaccgttaa tccagtcgtc gctacccttt atcgcaacct cattgactat
     5821 ttgtttatcc cacttaaaat tcgcgcgccc aaattaccag cctgagacca gcaccgacag
     5881 tgaaatacct cgccaaatat atcctgctga caatctgcct gctctccacg tcgatcaacg
     5941 ccattgctgc cccgcgcacc ctgctccgca gcgccagccg gattgaattc gccataaaag
     6001 agatgggggt tccggcaacc ggcgaattca gccgcgtcga ggcaagactc aatatggacc
     6061 cggccagaat ggaggcatcc ggcgccgaat tgcgcatcga caccggctcg ctgatcaccg
     6121 gaaataatga atgtaatctg ggtgatatcg tgaacaatga tattctagcc aagactctgc
     6181 tcacactggc ggcaaacgag ccacgagccc ctccgtcaca gacgaaaaac caatgacatc
     6241 tcatccgacc ataacgacca atcagacgac catccttcgc gccgagaatc ttgcgctgct
     6301 gttcgatgac ggccagaccc gtgcactgaa cgggctgaac gtcgagatca acgaaggcga
     6361 gttcgtcgcc atcgtgggcc ccagcggttg cgggaaatcc agcctgctca acctgatcgg
     6421 cacgctcgac atcccgacct cgggtgaaat ctatttccgc tcccggccct attcggcgat
     6481 ccgcgacctg tcgctgttcc gtcgccagaa catcggtttt attttccagt cgttctacct
     6541 gctgcctaca ctatcggcgc tcgacaacgt gctggttccc accatcggct gccccggtgc
     6601 ggctcaggag catgaagagc gagcccgctc cctgctgtcg cgactcggtc tggacaagcg
     6661 tctggatcac tttccgggca agctgtcggg cggcgaacgc cagcgcgtcg ccatcgcgcg
     6721 cgcgctgatc aacgatcccg acatcatcct agcggacgag cctaccggca gcctggatag
     6781 cgccaatgcc actcaggtac tggagttgat cgacggcgtc cgcaaggaga aggaattgac
     6841 cgtggtcatg gtcacccatg accgcagcgt atccgcccgc gccgaccgca tcatccgcat
     6901 gcgcgacggc cagatcgaat cggaggcaca accatgaacc tgttccgctt cgcgctcgac
     6961 aacctctatc gccgcccggc ccgcaccggc ctgacggttt ccgcgatctc gctcggcatc
     7021 gctgcagtcg tggcgctgac cagcatcgcc tggggctttg aagccagctg gcaaaaagcc
     7081 aacgatgccc gtggcaccga tctgatcgtg acccgcatcg ccagcgagaa caccatgccc
     7141 tcgccgtttg ccgccgagca ggtcggcaag accctgctga ccttcccgca tgtgaaagag
     7201 gtcgtcggcc tgctcagcga aatgctgacc atcagcgaag atgctccgcc ggtgttcgta
     7261 ttcggctggg cgcacggcag ctacctgtgg gatcacctca aactgcgcga cggccgctgg
     7321 gctaacagcg atgccgaagc ggtggtcgtg gtcggctcgc tggcctccga gctgctgcac
     7381 aagaaaatcg gcgacgcgct cgaaatcgag ggccgcgcct ttcaagtggt cggcatattc
     7441 gagagttcgg ccatgatcga gaacggcgcg gtgctgatga ccctgactca ggctcagcag
     7501 atcaccgaca agcccggcaa ggtcaacatc ctgaacatca agctagatag caataccagc
     7561 gagaccgaca tcgaagccat caaggcgcag gtgcgcaaag acctgcccgg cttcatcgcc
     7621 atcacctcgg gcgagctggt gcagaagaac gccgtggtgc gcatctccaa ggccatgagc
     7681 aatgccaccg tcctgatcgc cagcctggtc ggcgcactgg tggtgttcaa caccatgctc
     7741 atgagcatca acgagcgcac ccgcgagatc ggcatcctgc ttgccttggg ctggcagcgc
     7801 cgcaccatca tcaaactgat catcagcgaa tccatgatcc tcaccctgct gggcggcact
     7861 ttcggcgtcc tgctcggtgt cgcgatcacc tggggactgg agcatctgga gctgatgcgc
     7921 ggcaagatcg acgcggtgtt ctccacgcca ttcctgtttt cggtactcgg cctgtccgtc
     7981 cttctgggaa tctgcggcgg cctctatccg gccatcaagg cagcgcggtt gctgccttcc
     8041 aatgcgctca gacaagaatg aaacacatcg ccctgaggct cgccgccacc ctgctgctgg
     8101 ccgcatttgc gcagccgggt gcggcagccg acgctgccgc gcttgccgaa gcgatgcagc
     8161 aggcgcgtta ctccgacgga tttacggtgc ggatgaacat cgcgagcatt cagccgaatg
     8221 ggcgtcgcgc catgccattc aagctcgccg tcatcggtca gattgatgca aagcggcaac
     8281 gcctgctgat caaaggcatc tcaccggaca tggtgcgcaa ccgctttgtt gttgcagaac
     8341 ggaatgcgga tggccgtatc cgggcgatcg gctacaccga accgtcggcg gccgacatcg
     8401 gaaaggtcga tccgaccgca ccgctgttcg attccggcct ggtgctctgg gacatgctcg
     8461 ccccctggtg ggagtggcct cggcaaaaca tgggggaaac ggaacagatc gccggacgca
     8521 gctgcaccct cgtccgctcc caaaccgatg agaacttttc gcccatccgc gaggtcataa
     8581 gctgcgtgga tgaaaaggcg agactgtcat tgcgcaccca gttatttgac cgccgccata
     8641 ccctgatccg cacgattagc gtgaagcaga ccatgcgcaa ggattcgggg gcgctggcgg
     8701 ccaaaaagct gactatcaca gaccatgcag taaccgaggt cgaggtctac agcggcgacg
     8761 agcactacca agtcaccgcc gacaccttcg cccagttcga cgcctacagg gcggccgaga
     8821 agtagaggaa aaaatgcaac atctggaaca tctgacaacg agcgaagact tatccggcac
     8881 ctggtgcaac gccaatgccg tgacctccag catcatggag gcggtcagtc tcgtcacgcc
     8941 ggtgctggag aagtttttca tccgcaccgt caacgaaggc ctgtccgaac gaacctcgct
     9001 tgcgctcaga gagcgttgcc tgactttcat ccgcgaagag gccgaccatt cgcgagtaca
     9061 caagaagttc aatacttcgg tgctccacta tctggggcag acgccccccg gcctggcaag
     9121 gctcgaggcc ctgctggatg gcacgcgcaa ccgcctgtcg ctccccagtc gcctgttact
     9181 cgccgcagca ctggagcatt tcaccgcagt attgtccaaa gtctatctgg cacaggaggg
     9241 gcgtctggat tttcagtcgg cctttgcgca ggcgctgttc gcccatcacg cccgcgaaga
     9301 gcttgcccat cgttccgtcg tattcgacct gtggctcact ctgggcacca gcggtcgttt
     9361 taaacgctcg ctgacggtgt ttacgatcct gctggtgggc atcacttaca tcgccgtcgc
     9421 cgtaccctgg atactgcatc gcaagactgg caagttgagt gccacgctat cctcgctcct
     9481 cagcttcgcc gtgaagaatc ggtcgaacat cccgtcctac tcgccgctgg cggagctgtt
     9541 ctccttcgtc cgtcgcagct atcatccaga ccatctggtt gacgaccgcg caacccctgt
     9601 cgggcaacac cgcgcgtgaa cacccgcaca actatcacgc tgcacgggtt ctcgtcgccc
     9661 tgctgatctt cgccgccttc ccggtctgtc tatgagcgct cctgtggttc agcatgcagc
     9721 cattgcccga cctg
//
LOCUS       DASUIV010000062        10264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1038849, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000062 DASUIV010000000
VERSION     DASUIV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038849"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..422
                     /locus_tag="VFW59_02235"
     CDS             24..422
                     /locus_tag="VFW59_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEX5363061.1"
                     /translation="MSDLSTRPYLIRAIYEWCEDSGLTPYLAVRVDEFTEVPPAYIKD
                     GEIVLNMSADAVRNLELGNEYITCSGRFGGAPFNIIVPVGAVIGIFAKETGQGLVFQG
                     SDALPPGSSPVGGETEAKPKPNKPQLKIVK"
     gene            complement(486..1346)
                     /gene="rpoH"
                     /locus_tag="VFW59_02240"
     CDS             complement(486..1346)
                     /gene="rpoH"
                     /locus_tag="VFW59_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX5363062.1"
                     /translation="MNAVMNFPMPSAVGSLESYIQAVNRFPILSQEEEIALATRLRNE
                     HDLDAARQLVLSHLRVVVSVARGYAGYGLPQADLIQEGNIGLMKAVKRYDPDRGVRLV
                     SFALHWIRAEIHEFVVRNWRMVKIATTKAQRKLFFNLRSLKQGLAPLKPQQITEIAQQ
                     LNVSEHDVVEMEARFAGHEVALDPSGSDEDDSYAPIQYLADDADHEPSRILESAEQEH
                     LQTSGLRNALEGLDERSRRIVEARWLTEGESATLHELAAEFNVSAERIRQIEQKALGK
                     MHACLALKAS"
     gene            complement(1533..2441)
                     /gene="ftsX"
                     /locus_tag="VFW59_02245"
     CDS             complement(1533..2441)
                     /gene="ftsX"
                     /locus_tag="VFW59_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX5363063.1"
                     /translation="MNQAFAQHLAVLRAVLRRMFASKVGGLLNILVIGITLSLPSGLY
                     LLLQNTQALVEQLSGSPQVSLFLTLDTQPDDIADLRKQLMANDAIGSIEFVARDQALE
                     QLKQSTGLSNVIGSLEKNPLPDAFIVSPRKGDPQTLNALRDELAQLPKVEQAQLDSAW
                     AYKLEALLKFGRMGVLILASLLSLALIAVTFNTIRLQILTQRDEIEVSRLIGATNGFI
                     RRPFLYFGALQGLLGGLMAWLIVATSLLLLNQPLRELSQLYASQFMLNPLNLGDSLSL
                     LLFSLYLGWLGAWLSVTRHLSQIEPC"
     gene            complement(2438..3130)
                     /gene="ftsE"
                     /locus_tag="VFW59_02250"
     CDS             complement(2438..3130)
                     /gene="ftsE"
                     /locus_tag="VFW59_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX5363064.1"
                     /translation="MISFNQVYKRYPGGYEALKNVSFDINEGEMVFLTGHSGAGKSTL
                     LKLIAAIERPNSGIVLVNNQNVGALKGGALPYLRRNLGILFQDHKLLFDRNVFDNVLL
                     PLQICGFDHRESSKRVRAALDKVGLLKREKSNPIALSGGEQQRLCIARAIVNRPSILL
                     ADEPTGNLDAAYAQEIMSIFKSFHQVGVTLLISTHDESVLQDSGVRRLALRQGELLAD
                     ESAGHSTGSERT"
     gene            complement(3127..4104)
                     /gene="ftsY"
                     /locus_tag="VFW59_02255"
     CDS             complement(3127..4104)
                     /gene="ftsY"
                     /locus_tag="VFW59_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX5363065.1"
                     /translation="MFSFLKKNSAESSNAKTDGGWVARLRSGLARTGNQLTDLFGRGG
                     KIDDELYEELETILVTSDVGMTATNALLADLRLRVREQRLTEAAELKEALAHCMLKLL
                     KPLAQPLQAQIFKSNEPHRPFVIMMCGVNGAGKTTSIGKLAKYLQGQGLSVLLAAGDT
                     FRAAAREQLMTWGERNNVTVIAQQSGDAAAVIFDAVQAAQARKIDVVLADTAGRLTTQ
                     AHLMEEIKKVKRVIAKVLPDAPHEVLLVLDANTGQNALSQVKAFDEALGVTGLILTKL
                     DGTAKGGVIAAIAKAHPIPVRFIGVGEGLDDLRPFVAEDFVTALLGLEA"
     gene            4178..5533
                     /locus_tag="VFW59_02260"
     CDS             4178..5533
                     /locus_tag="VFW59_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5363066.1"
                     /translation="MRIYLLGIALLFSVAAQAEVFERTLGNGLKVIVKEDHRAPVVVQ
                     QIWYKTGSMDERTGVTGIAHLLEHLMFKGTAAVPAGEFSRRIAAAGGRENAFTSYDYT
                     AYFQQLHKSSLPLAMQLESDRMRNLTLSPAEFAKELKVVMEERRWRTDDDAHALLQET
                     MMAAIYQQHPYQHPVIGWMSDLESLSVGDARAWYDRWYAPNNATLIVAGDVQADAVFA
                     LAQRYYGAIPRRAMPRVRNFTEQKQVGIKRLVVKAPAELPHLVMSFHAPVLRDPAKDS
                     QPYALEILAGVLDGNESARLNKHLVREQQVASAVGGGYDSFARGPGLFTLEATPSEGK
                     TVVDVEAALRREISQLVQEGISEEELRRVKAQVMASEVYKLDSLFYQAMQIGQLESIG
                     LGHRAIPLMLEKLQAVTAEQVRQVAEEFLLDDNLTVAVLDPQPLSGKPKRPPQGASHV
                     R"
     gene            5523..6851
                     /locus_tag="VFW59_02265"
     CDS             5523..6851
                     /locus_tag="VFW59_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5363067.1"
                     /translation="MYVKTIFLAALCCIGSSAFATPGIQHWQSASGAKVLFVENHDIP
                     MLDVAVSFPAGSSFDVADKSGVAALTQSLLDLGAEGLTEDDIARGMADIGAQFGGSFD
                     QDRSSMSLRTLSTPAEREQALDIMARVLQRPLFRGEILAREKARVIAALKEAETKPES
                     IASKAFDKAVFGAHPYGLPTSGEVVSVEKLFVQDLRDFYASHYQARTAVVAIMGDVSR
                     AQAEVIAQQLTAQLPTGAAPAPLPAVAHMAASEQRIAHPASQSHILLGAPGMARNDPD
                     YFPLYVGNYILGGGGFVSRLMNEVREKRGLAYSVYSYFMPMKQPGAFQIGLQTKKEQA
                     DQALQLVRQTLSEFIANGPTEKELVAAKQNIVGGFPLRIDSNRKILDYLSVIGFYDLP
                     LTYLKDFTRQIEQVTVAQIRAAFARHVDPQAMATVIVGAPEPELKAGAAK"
     gene            6855..7400
                     /gene="rsmD"
                     /locus_tag="VFW59_02270"
     CDS             6855..7400
                     /gene="rsmD"
                     /locus_tag="VFW59_02270"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX5363068.1"
                     /translation="MAKVNKVRIIGGDLRSRVISFPDAEGLRPTPDRVRETLFNWLGQ
                     TLYGRSCLDLFAGSGALGFEAASRGAERVTMVEQNPPVARALRDNLKSLAVANVFVHV
                     QDGLEFALRDKQRYDVIFLDPPFGSDYLPKLLEIMPQRLNEHGVLYVESGAALDVPPQ
                     WQVKKSGKAGQVHYQLMGLAE"
     gene            7397..7867
                     /gene="coaD"
                     /locus_tag="VFW59_02275"
     CDS             7397..7867
                     /gene="coaD"
                     /locus_tag="VFW59_02275"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5363069.1"
                     /translation="MIKVVYPGTFDPITRGHEDVVRRAAGLFGEVIVAVAESRAHTLF
                     TLEERVAMAREVFADFTNVRVEGFNGLLMNFVQAHDARVVLRGLRAVSDFEYEFQLAG
                     MNRNLYPEVETLFLTPAEQYTFISATMVREVARFGGDVGKFVSPLVATRLQQKN"
     gene            7892..8146
                     /locus_tag="VFW59_02280"
     CDS             7892..8146
                     /locus_tag="VFW59_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX5363070.1"
                     /translation="MALMITDECINCDVCEPECPNDAISQGDEIYVIDPNKCTECVGH
                     YDTPQCVEVCPVDCIPKDPAHVESEAQLRVKYDRLMAAKG"
     gene            8301..8534
                     /locus_tag="VFW59_02285"
     CDS             8301..8534
                     /locus_tag="VFW59_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX5363071.1"
                     /translation="MPIYAYACSSCGSQQDVMQKMSDAPLTTCPQCGKETFAKQLTAA
                     GFQLKGNGYYVTDFKDKPKSTPCASSGSCPMAS"
     gene            8534..9154
                     /locus_tag="VFW59_02290"
     CDS             8534..9154
                     /locus_tag="VFW59_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX5363072.1"
                     /translation="MKKYLITGLLVWVPLGITAWVLNLTIGTMDQTLLLLPEQWHPDR
                     VLGIHIPGLGIILTLVVVLVTGLLIRNVFGQRLWDASERAMLHVPFVGSIYKGVKQVS
                     DTLLSGNGNSFRKVLLVRYPHPQAWSLAFQTNVPREVSSLFDDEYVAVFIPTTPSPVN
                     GFYFYVRRADTIELDMTVDVALRTIVSMGVVASPEIVHAAQNIKQD"
     gene            9176..>10264
                     /gene="aspS"
                     /locus_tag="VFW59_02295"
     CDS             9176..>10264
                     /gene="aspS"
                     /locus_tag="VFW59_02295"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5363073.1"
                     /translation="MRTHYCGLLNTDQLDQTVSICGWAHRRRDHGGVIFIDLRDREGL
                     AQVVCDPDRAEMFKIAESVRSEFVLRITGKVRRRPAGTTNSNITSGEIEILCHEIEVL
                     NSAVTPPFQLDDENLSENVRLTHRVIDLRRPQMQKNMMLRYKVAMAFRRFLDSRGFID
                     IETPMLTKSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQMLMVAGFDRYYQVTKCFR
                     DEDLRADRQPEFTQVDIETSFLNETQITGLMEEMIRSIFKETMDVDLPNPFPRMSYAE
                     AMARFGSDKPDLRVTLELTEVTDAVKDVAFKVFAGVANSETGRVAALRVPGGAAFTRG
                     EIDEYTKFVGIYGAKGLAYIKVNDVTQLN"
BASE COUNT         1985 a         2927 c         3290 g         2062 t
ORIGIN      
        1 tccgaaaggg cgatgcgtaa ataatgagcg atttgtcgac gcgcccctat ctgattcgtg
       61 ccatttacga atggtgcgag gatagtgggt tgactcccta tctggctgtc cgtgtcgatg
      121 aattcaccga ggtgccccct gcctatatca aggacggcga aatcgttctt aatatgagtg
      181 ccgatgccgt gcgtaatctt gagttgggca acgagtacat tacctgcagc ggacgtttcg
      241 gtggcgctcc ttttaatatc atcgtgccgg tcggggctgt catcggcatc ttcgccaagg
      301 agacggggca agggctggtg tttcagggca gcgatgcgct gccaccagga tcgtcgcctg
      361 tcggcggcga gactgaagcc aagcctaagc cgaacaagcc gcagttaaag atcgtgaagt
      421 gagttgttcg caggaaacaa aaaagccgca gactagtctg cggctttttt gtttggtctt
      481 cagcctcagg aggctttcag cgccaggcat gcatgcatct tgcccagcgc cttctgctcg
      541 atctggcgga tacgttcggc agaaacgttg aattctgcgg ccagttcgtg cagtgtcgcc
      601 gactcaccct cggtcagcca gcgagcttca acgatgcggc gactgcgttc gtccagccct
      661 tccagtgcat ttcgcaagcc cgatgtctgc agatgttctt gctcggccga ttccaggatg
      721 cgcgacggtt cgtgatcggc atcgtcggcc agatactgga tcggagcata gctgtcgtct
      781 tcgtcgctgc cggaaggatc cagagcaact tcatggccgg caaaacgcgc ctccatctcc
      841 accacgtcgt gttcgctgac attgagctgt tgcgcaatct cggtgatctg ttgcggcttg
      901 agcggggcga gcccctgttt caggctgcgc aggttgaaga acagcttgcg ctgcgccttg
      961 gtcgtggcga tcttgaccat gcgccagttg cgtactacga attcgtgaat ctcggcgcgt
     1021 atccagtgca gcgcgaacga aaccagccgc acgccccgat ccgggtcgta gcgttttacc
     1081 gccttcatca ggccgatgtt tccctcctga atcagatcgg cttgaggcaa cccataaccc
     1141 gcataaccac gcgcaacgct gaccaccacg cgcagatgcg acagcaccag ctgacgcgca
     1201 gcgtccagat cgtgttcgtt gcgcaaccgg gtcgccagcg cgatttcctc ttcctgagac
     1261 aggataggga agcggttcac cgcctggata taactctcaa gactgccaac agcagacggc
     1321 attggaaaat tcataacagc attcatgaca tttcctcctt cgcaggaatt ctagcactcg
     1381 cgcgatgaga gtgccaagaa agtgatgagt tccctaacgg acatggcaac tttgtcatcc
     1441 ctgatgatga acttgctacg cccgtttccc cctatccaac tttcccccgc atgcaggaga
     1501 aagggcaaac gaatcgctgc gcgagttttg cgttaacagg gttcgatctg ggacaggtgg
     1561 cgtgtgaccg acaaccatgc tcccagccac ccaaggtaga gcgagaacag cagcagcgac
     1621 aggctgtcgc caagattgag cgggttcagc atgaactgac tggcatagag ttgcgagagc
     1681 tcgcgcagag gctgattgag caacagcagg ctggtggcaa cgatcagcca ggccatcagg
     1741 ccgccgagca ggccttgcag ggcgccgaaa tacaggaagg ggcgcctgat aaaaccgtta
     1801 gtcgcgccga tcagccggga cacctcgatt tcgtcgcgct gggtaaggat ttgcaggcgg
     1861 atcgtgttaa aggtcacggc gatcagcgca agactgagca ggctggcaag gatcagcact
     1921 cccatgcggc caaatttaag caaggcctcc agcttgtaag cccaggctga atcgagttgg
     1981 gcctgttcta ccttgggaag ctgcgccagt tcatcgcgca atgcattgag tgtctgcggg
     2041 tctccctttc tgggcgagac gatgaaggcg tcgggcagcg ggtttttttc caggcttccg
     2101 atcacgttgg acaggccggt gctctgtttc aactgttcca gagcctgatc cctggcgacg
     2161 aattctatgg agccgatggc gtcgttcgcc atcagctgtt tgcgcagatc ggctatgtcg
     2221 tccggttgcg tatccagcgt caggaacagg ctgacctgcg gtgagccgga aagctgttcg
     2281 accagcgcct gagtattctg cagcaacaga tacaggcccg agggcaggct gagcgtgatg
     2341 ccgatcacca gaatgttgag caggccccct actttggagg cgaacatgcg gcgcagcacg
     2401 gcgcgcagca cggcaagatg ttgggcgaag gcctgattca tgttcgctcc gagcctgtcg
     2461 aatgacccgc agattcatcg gcgagcagct ccccttgtct cagcgcgagc ctgcgtacgc
     2521 cactgtcttg caatacgctt tcgtcgtggg tcgagatcag cagggtcacg ccgacctgat
     2581 ggaaagactt gaagatagac atgatctcct gcgcgtaggc ggcatcgaga ttgccggtcg
     2641 gttcgtcggc aagcaggatg gaagggcggt tgacgatggc acgggcgatg cacaggcgct
     2701 gttgttcgcc gccggacagc gcgatcggat tggacttttc gcgtttcagc agtccgacct
     2761 tgtccagcgc ggcacgcacc cgcttgctgc tctcacgatg gtcgaagccg cagatttgca
     2821 gtggcagcag cacgttgtcg aatacgttgc ggtcgaacag cagtttgtga tcctggaaca
     2881 ggatgccgag gttgcggcgc agatagggca atgcgccgcc cttgagcgcg ccgacattct
     2941 ggttgttcac cagcacgatg ccggagttag ggcgttcgat ggcggcgatg agcttgagca
     3001 gggtgctctt gcctgcaccg gaatgaccgg tgaggaaaac catctcgcct tcgttgatgt
     3061 cgaacgacac gttcttcagc gcctcatagc cgccggggta gcgcttgtat acctgattga
     3121 agctgatcac gcttccagtc ccagcaaggc ggtcacgaag tcttccgcga cgaacgggcg
     3181 caggtcatcc agcccctcgc ccacgccgat gaaccgcacc ggaatcggat gggccttggc
     3241 gatcgcggcg atcacaccgc ccttggcggt tccatccagc ttggtcagga tcaggccggt
     3301 gacgccgagc gcctcgtcga aggccttgac ctgagagagc gcgttctggc cggtgttggc
     3361 gtccagcacc agcagcactt catggggagc gtcgggcagt accttggcga tcacgcgttt
     3421 gaccttcttg atctcttcca tcaggtgagc ctgtgtcgtc aagcgtccgg cggtgtcggc
     3481 cagcaccacg tcgatcttgc gcgcctgtgc ggcctgcacc gcgtcgaata tcacggcggc
     3541 ggcatcgccg ctctgttgcg cgatcacggt gacgttgttg cgttcgcccc aggtcatcag
     3601 ctgttcgcgc gccgccgcgc ggaaggtgtc gcctgccgcg agcagcaccg acaggccctg
     3661 gccttgcagg tatttcgcga gcttgccgat ggaggtggtc ttgcccgcac cgttcacgcc
     3721 gcacatcatg atgacgaaag gcctgtgcgg ctcgttggat ttgaaaatct gggcctgtag
     3781 aggttgcgcc agcggcttga gcagcttgag catgcagtga gcgagcgctt ccttgagctc
     3841 ggcggcttcg gtgaggcgct gctcccgtac ccgaaggcgc aggtcggcga gcagtgcgtt
     3901 ggtcgcggtc atgccgacat ccgaggtgac caggatggtc tccagttcct cgtacaactc
     3961 gtcgtcgatc ttgccgccac ggccgaacag gtcggtgagc tggttgccgg tgcgcgccag
     4021 cccggacctc aggcgcgcga cccagccacc gtccgttttg gcgttcgatg attcggctga
     4081 attctttttt agaaagctaa acatttaaaa ggggcgggaa aatatcgcat aatgcgcgcg
     4141 gcattttatg ccgattacac cttctggggc taggaacatg agaatttatt tgctgggaat
     4201 cgcgttgctg ttcagcgtcg ccgcgcaggc ggaagtgttc gagcgcacgc tggggaacgg
     4261 tctgaaggtc atcgtcaagg aagatcaccg cgcgccggtg gtggtccagc agatctggta
     4321 caagaccggc agtatggacg agcgtaccgg cgtgaccggc atcgcgcacc tgctggagca
     4381 tctgatgttc aagggaacgg cagcggttcc tgccggcgag ttttcccggc gtatcgctgc
     4441 ggcgggtggt cgcgagaatg ccttcaccag ctacgattac accgcctatt tccagcaatt
     4501 gcacaagtcc agtctgccgc tggcgatgca actggagtcc gaccgcatgc gcaatctgac
     4561 gctgtcgccg gcagagttcg ccaaggagct caaggtggtg atggaagagc gccgctggcg
     4621 caccgacgac gacgcccatg cgctgttgca ggaaaccatg atggcggcga tctaccagca
     4681 gcatccttac cagcatccgg tgatcggctg gatgagcgat ctggaatcgc tatcggtggg
     4741 cgatgccaga gcctggtacg accgctggta tgcgccgaac aacgccacgc tgatcgtggc
     4801 cggcgacgtg caggccgacg cggtgtttgc actggcgcag cgttattacg gggcgatccc
     4861 gagacgtgcg atgccacggg tgcgaaattt caccgaacag aaacaggtcg gcatcaagcg
     4921 tctggtggtg aaggctcccg ccgagttgcc gcatctggtg atgagttttc atgcgcctgt
     4981 gctgcgcgat ccggcaaagg acagccagcc ctatgcgctg gagattctgg cgggcgtgct
     5041 ggacgggaac gaatcggcgc gtctgaacaa gcatctggtc cgcgagcagc aggttgccag
     5101 tgcggtgggg ggcggttatg actcgttcgc gcgcggcccg ggcctgttca cgctggaggc
     5161 aacgcccagc gaaggcaaga cggtggtcga tgtcgaggcc gcgctgcggc gcgagatatc
     5221 gcaattggta caggaaggca tcagcgaaga agagttgcgg cgcgtcaagg cccaggtcat
     5281 ggcgagcgag gtctacaagc tcgactcgct gttttatcag gcgatgcaga tcggtcagct
     5341 ggagagcatc gggctgggtc accgcgccat tcccctgatg ctggaaaaac tgcaggccgt
     5401 cacggcggag caggtgcgcc aggtcgccga ggaatttttg ctggacgaca atctgaccgt
     5461 cgcggtgctc gatccgcagc cgctttccgg caaaccgaaa cgtccgccac aaggagcatc
     5521 gcatgtacgt taagaccatt tttctggcgg cgctgtgctg catcggcagc agcgcgtttg
     5581 ccacccccgg tattcagcac tggcagagcg ccagcggcgc gaaggtgctg ttcgtggaga
     5641 accacgacat tccgatgctg gatgtggcgg tgagctttcc ggcgggtagc agcttcgatg
     5701 ttgccgacaa gtcgggcgtg gcggcgctga cccagagcct gctcgatctg ggcgcggagg
     5761 ggttgaccga ggacgatatc gcgcgcggca tggccgatat cggcgcgcag ttcggcggca
     5821 gcttcgatca ggaccgttcc agtatgtcgc tgcgcaccct gagcaccccg gccgaacgcg
     5881 agcaggcgct ggacatcatg gcgcgcgtgc tgcaacgacc gctgtttcgc ggcgagatac
     5941 tggcgcgcga gaaggcgcgc gtgatcgccg cgctgaaaga agccgagacc aagcccgaaa
     6001 gcatcgccag caaggcgttc gacaaggcgg tgttcggtgc gcatccctat ggcttgccga
     6061 cctccggtga ggtcgtgagt gtggagaagc tgttcgtgca ggatctgcgc gatttctacg
     6121 cgtcgcatta tcaggcccgt acagccgtgg tcgccatcat gggtgatgtg tcccgcgcgc
     6181 aggccgaggt gattgcgcag cagcttacgg cacagttgcc gacgggtgcg gcgcccgctc
     6241 ccttgccggc ggtggcgcac atggccgcga gtgagcagcg catcgcccat cccgccagcc
     6301 agagtcatat attgctcggc gcgccgggca tggcacgcaa cgatcccgat tactttccgc
     6361 tgtatgtcgg caactatatt ctcggcggcg gcggtttcgt gtcgcggttg atgaacgagg
     6421 tgcgcgagaa gcgcggtctg gcctacagcg tatacagcta cttcatgccg atgaagcaac
     6481 ccggcgcgtt tcagatcggc ctgcagacca agaaagagca ggccgatcag gcgctgcagc
     6541 tggtgcgcca gaccttgagc gagttcatcg ccaacgggcc gaccgagaag gagctggtcg
     6601 ctgccaagca gaacatcgtc ggcggtttcc cgctgaggat cgacagcaac cgcaagatac
     6661 tcgattacct cagcgtgatc ggtttctacg acctgccgtt gacctatctg aaagatttta
     6721 cgcgccagat cgagcaggtt acggtggcgc agattcgcgc tgcctttgcg cgtcacgtgg
     6781 atccgcaggc aatggccacg gtgatcgtcg gtgctccgga gcccgagttg aaagccgggg
     6841 cggcgaagta agcgatggcg aaggtaaaca aggttcgtat catcggcggc gatctgcgca
     6901 gccgcgtcat tagcttcccg gatgcggagg ggttgcgtcc cacaccggat cgcgtgcgcg
     6961 agacgctgtt caactggctg gggcaaacct tgtacgggcg cagctgtctc gatctgttcg
     7021 cgggcagcgg cgcgctgggc ttcgaggccg cttcgcgcgg ggcggagcgg gtgacgatgg
     7081 tggagcagaa tccgcccgtt gcgcgcgctt tgcgggataa ccttaagtcg ctagcggttg
     7141 ctaacgtttt cgttcacgtt caggatgggc tagaattcgc ccttcgcgat aaacagcgct
     7201 acgacgtgat ctttctggat ccgccgttcg gcagcgatta tttgccgaag ctgctggaga
     7261 tcatgccgca gcggttgaac gagcatggcg tgctgtacgt cgagtccggt gcggcgctgg
     7321 atgtgccgcc gcagtggcag gttaagaagt ccggcaaggc gggacaggtg cattaccaac
     7381 tgatggggct ggctgaatga tcaaggtggt atatccgggt acgttcgatc ccatcacgcg
     7441 cggacacgag gatgtggtgc gccgcgcggc agggctgttc ggtgaagtca tcgtcgcggt
     7501 ggcggagagc agggcgcata ccctgttcac gctggaggag cgggtggcaa tggcgcgcga
     7561 ggtgtttgcc gattttacca acgtgcgcgt ggaaggtttc aacggcctgc tgatgaattt
     7621 tgtgcaggcg catgatgcgc gcgtggtgtt gcgcggcctg cgggcggtgt cggacttcga
     7681 gtacgagttc cagctggcgg gcatgaaccg caacctgtac ccggaggtcg agacgctgtt
     7741 cctgacgccg gccgagcagt acactttcat ctcggcgacc atggtgcgcg aggtcgcgcg
     7801 cttcggcggg gatgtcggca agttcgtttc gccgctggtg gcgacaaggt tgcagcaaaa
     7861 aaattagaac gattttaatg aggagagaaa catggcactg atgattaccg acgaatgtat
     7921 caactgcgac gtgtgcgagc ccgagtgccc gaacgatgcg atttcgcagg gtgacgagat
     7981 ttatgtgatc gacccgaaca agtgtaccga atgcgtgggt cactatgaca ccccgcagtg
     8041 cgtggaagtc tgcccggtgg actgtatccc caaggatccc gcccacgtag agagcgaggc
     8101 gcagttgcgc gtcaagtatg atcgcctgat ggcggccaag ggctgattat cgcgctgcac
     8161 gacaagccgc acagcttggc tgtgcggctt gtgcttttcc ggggggtgat tcggcagggg
     8221 gttgggcttc gcccactcac ggctggacgc atccctaatg tataatgcgc gcctttggaa
     8281 ttgcccggta ggagagagaa atgccgattt atgcctatgc ctgttccagc tgcggttcgc
     8341 agcaggatgt gatgcagaaa atgagcgatg cgccgctcac cacttgtccg cagtgcggca
     8401 aggagacttt tgccaagcag ttgaccgcag ctggttttca gttgaagggc aacggttact
     8461 atgtcaccga cttcaaggat aagcccaaga gtacgccctg cgccagttcg ggttcttgcc
     8521 cgatggcaag ttgatgaaaa aatacctcat caccgggctg ctggtctggg ttccgctcgg
     8581 catcaccgcc tgggtgctga acctgaccat cggtacgatg gatcagaccc tgctgctgtt
     8641 gcctgagcaa tggcatccgg acagggtgct gggcatacat attcccggtc tgggcatcat
     8701 cctgacgctg gtcgtggtgc tggtcaccgg cctgctgatc cgcaacgtgt tcggtcagcg
     8761 gctgtgggat gcttccgaac gggcgatgct gcatgtgccc ttcgtcggca gcatctacaa
     8821 gggcgtgaag caggtcagcg acacgctgct gtcgggtaac ggcaactcgt tccgcaaggt
     8881 gctgctggtg cgttatccgc atcctcaggc atggtcgtta gccttccaga ccaatgtgcc
     8941 gagagaggtg agcagcctct tcgacgacga atatgtggcg gtgttcattc ccaccacgcc
     9001 cagcccggtg aacggttttt atttttatgt gcgccgcgcc gacaccatcg agctggacat
     9061 gaccgtggat gtggcgttgc gcaccatcgt gtcgatgggc gtggtggcct cgcccgagat
     9121 cgtgcatgcg gcacagaaca tcaagcaaga ctaactttta ccaagaaatc gaatcatgcg
     9181 aactcattac tgcggactac tcaacaccga ccaactcgac cagaccgtca gcatctgcgg
     9241 ctgggcgcac cgccgccgcg accacggcgg ggtgatcttc atcgacctgc gcgaccgcga
     9301 aggcctggcg caggtggtgt gcgatccgga tcgtgccgaa atgttcaaga tcgccgaatc
     9361 ggtgcgcagc gaattcgtgt tgcgcatcac aggcaaggtt cgccgccgtc cggcgggcac
     9421 gaccaattcg aatatcacca gcggcgagat cgagatcctg tgccacgaga tcgaggtgct
     9481 gaacagcgcg gtgacgccgc cgttccagct ggacgacgag aacctgtccg agaacgtgcg
     9541 cctgacccat cgcgtgatcg acctgcgccg tccgcagatg cagaagaaca tgatgctgcg
     9601 ctacaaggtg gcgatggcgt tccgccgttt cctcgacagc cgaggtttca tcgacatcga
     9661 gacgccgatg ctgaccaagt ccacgccgga aggagcgcgc gactatctgg tgccgtcccg
     9721 cgtgcatccg ggcgaattct ttgcactgcc gcagtcgccg cagctgttca agcagatgct
     9781 gatggtggcc ggcttcgacc gttactatca ggtcaccaaa tgtttccgcg acgaagacct
     9841 gcgcgccgac cgccagccgg aattcaccca ggtggatatc gagacctcgt tcctgaacga
     9901 gacgcagatc accgggctga tggaagagat gatccgcagc atcttcaagg aaaccatgga
     9961 tgtagatctg cccaacccgt tcccgcgcat gagctatgcc gaggcgatgg cgcgcttcgg
    10021 ttcggacaag cccgacctgc gcgtgacgct ggaactgacc gaagtcaccg atgcggtgaa
    10081 ggatgtcgcg ttcaaggtgt tcgccggtgt ggcgaattcc gagaccggcc gcgtggcagc
    10141 cttgcgcgtg ccgggcggcg cggcattcac ccgtggcgag atcgacgagt acaccaagtt
    10201 cgtcggcatc tacggcgcga aggggctggc ctacatcaag gtcaacgacg tcacgcaact
    10261 gaac
//
LOCUS       DASUIV010000063         9118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1038869, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000063 DASUIV010000000
VERSION     DASUIV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038869"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFW59_02300"
     CDS             <1..282
                     /locus_tag="VFW59_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEX5363074.1"
                     /translation="GNGSGFGGGNRSSAFNGNGNNGGAGFHHSAPDSRHSHAGHKEGQ
                     SHTGFAGQAQPRSSERSFGNANRAPSSGAPRRSGGGDRPAGGQGRTNKW"
     gene            509..1846
                     /locus_tag="VFW59_02305"
     CDS             509..1846
                     /locus_tag="VFW59_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5363075.1"
                     /translation="MPNIAEIAESQLDDPAYLKAITELGDSVPLVTTQNLNSSTGIKL
                     VNAGTRFNSSLYNKLLQHKLVPTLDQSLAIEDAVSNNSLAAELPTILAEEGWLQPMLA
                     TMLNHEIFEQILTKLPLHPTAAFKLTVMRERHPGLFRRSICLTLICIYLGIRCQMDSN
                     KLVQLATAALLHDIGMLHIDPALLAEGYTLNNAERRHLYAHPLTAWMILKKCTDYSPA
                     VLDAVLQHHERLDGSGYPQGLAGNNISLNGQIIAVAEIITSRNGNMTSAFDKMRLEAM
                     LKLNSRRYGRRLIGYLDVFYRSDEAVPACTDAYKQLIRNQLLCTQSAFSEWEKLWDAH
                     YQGYATFDSINRRMLNLKMEVLDAGLAPLDIDESLDGIEDSPRACFEMHILLDEVIWQ
                     LQSVLDEIRRRWPDINGEGPASCAIHILNWINATEQNIKSNKLTNPAGKSRGK"
     gene            1875..4721
                     /locus_tag="VFW59_02310"
     CDS             1875..4721
                     /locus_tag="VFW59_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5363076.1"
                     /translation="MKLEQARRLTSRFSNWFSALHPRNSLVAQISYAMALVSIVLSVA
                     LSYYAASLSRQQIEQELGRNFVHRAQNIVDVLDRGMFERYREVQIVATLSDIRNPAVS
                     IDQKRVVLEKLQQTFDAYAWVGICDLQGKGIVGTGKYLEGKDLSKRPWCKQGRDKPYV
                     GDVHDALLLSKILPNPSGENFYLVDVAAPVIDKSGALQGVLCGHIYWKWAEELLDSKK
                     NESFEILLLSRDGLVLTGPEQNRSELKKLAPDSWSRIINGKPGQDTLLDRWSNGKQYL
                     VGYAHDAGYRDYPGLGWIALVRQDAATAFAPARTLQLRILLAGVMLGIFFIFFGAWMA
                     RRIATPISRITAAAEKIAAGEFEYDAPPADGDGEVAHLSASIHTMVSNLTREILDRKK
                     AEEQLRLSAAVFANNSEAIVITDAHNDIVRVNDAFTRISGYSADEVIGLNPRIFSSGN
                     MDEKFYQKMWATLLENNGWTGEIWNKRKNGEIYPEWMILSLVRDEAGNITNHIAIYSD
                     ITVRKQEEEQIQFLANHDVLTSLPNRTLLTDRLEQALAFAERNKNKVAVLFIDLDHFK
                     NINDSLGHDVGDNLLRQAALRISACFRRTDTIARLGGDEFVAVLPEISSDNEVISLVE
                     KMLHAFGEKFMVNEHQLSITPSIGISLFPDDGLKPVTLLRNADIAMYRAKAQGRNTLQ
                     FYRPEMTAHATERLQLEMQLRQAIDNGELFLHYQPQVDLNSGDMIGMESLIRWKHPQQ
                     GMISPARFIPIAEESGLILEIGTWVLREACMQGRIWQKQGYKVVPIAVNVSGVQFKRG
                     QITEIVHNILDETGFDPHLLDLEITESVLMASGEASLDIMNSLKALGLTLSLDDFGTG
                     YSSLSRLKSLPLDYLKVDQSFVRDIATDPNDAAIVRAILSMSHEMNLKVIAEGVETTE
                     QLEFLRSLKCPTFQGYLFSRPIPPEEIELYLKTA"
     gene            complement(4804..6000)
                     /locus_tag="VFW59_02315"
     CDS             complement(4804..6000)
                     /locus_tag="VFW59_02315"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5363077.1"
                     /translation="MYIHDLNCGWIDHPFARNRFLIDDTAVFNKIIETGVTELYIDTE
                     LGLDVRATGLMDSEGSSAELEAGAEIVPPVCEQSTFVPKIIANLSPVGKFNHAKAVCS
                     NASKLMQEIMRDVRLGNQVDLKQCEPMIDGIVESMYSFPSALLPLAQIKTRDEYTFQH
                     SVSVAALAVAFGRVLDMPHDEIRQLAMGGLLHDVGKAQVPGRVLNKPGKLDEAEFALM
                     KSHVVNTAQLIKEVKGISTIAFNAAAQHHERYDGTGYPNGLKGDEISLHGQMLAIVDV
                     YDAITSIRVYHKGMPPTEALKKMYEWSGAHFNPKLVQAFIKGIGIYPAGSLVRMESDK
                     LGIVREVVPDRLLQPIVQLIYDCKKSSHMSPETVDLAASHDKIKSHESFEKWGIDQAR
                     WATAHI"
     gene            complement(6119..6472)
                     /locus_tag="VFW59_02320"
     CDS             complement(6119..6472)
                     /locus_tag="VFW59_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363078.1"
                     /translation="MQLPELKLPRLPSYLVSLFQLVTMLSSESLCRTDLIVAMTGGRI
                     KLKRVEEIVGLNENQSFSIQVEMIRQMQSENGQVACYATPATVGCSKKQSECSWRHDC
                     FHDAEEAGERRNKEA"
     gene            complement(6738..7142)
                     /locus_tag="VFW59_02325"
     CDS             complement(6738..7142)
                     /locus_tag="VFW59_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5363079.1"
                     /translation="MAFGNYSDDNMMSEINVTPLVDVMLVLLVVFIITAPLLAPQALK
                     INLPRTDSVQQSDKPQQVSLAVDAQGNLELDNRHVSDAGLAELLHQRATDPQFQLQIE
                     ADNAVPYGRIAELMALAQHAGVAKLSFITLAK"
     gene            complement(7147..7803)
                     /locus_tag="VFW59_02330"
     CDS             complement(7147..7803)
                     /locus_tag="VFW59_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5363080.1"
                     /translation="MTHLFSSDSPIVAGTLLLLLLLSVATWSIALFKLRRQVQDRKRN
                     RLFLGAFWAARDWQAAAQMAGNGEGDVARLAQSGFAELKALDADRQDLMHTGAPQEVL
                     ERQLRQQVQNLQRYHERGLAELATIGSTAPFVGLFGTVWGIMHALQNIGQSGSASLDV
                     VAGPIGEALVATAIGIATALPAVLAYNYFLRNLRVRITELDNFAHDFMRLALKHHFKG
                     "
     gene            complement(7800..8471)
                     /locus_tag="VFW59_02335"
     CDS             complement(7800..8471)
                     /locus_tag="VFW59_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX5363081.1"
                     /translation="MTAVAMNRTDPFPAAALSERVIVIALVVAVHLGLASAWMMQPER
                     ARVAVSEMSVSVAIRQAEVAQAQAQPEPVPPKPRPHSERSEKPVPKQTEQKAPGAVAQ
                     QETPVQAAPPAQAAAPSVAAAPVFDTVPDYRASYLNNPRPSYPMVARRMGWEGRVVLN
                     VEVRSAGDCGEVSVFQSSGHEVLDNAALRTVKSWHFVPASRAGHPVTQWFKVPIQFSL
                     KENEV"
     gene            complement(8543..>9118)
                     /locus_tag="VFW59_02340"
     CDS             complement(8543..>9118)
                     /locus_tag="VFW59_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5363082.1"
                     /translation="TACTATCLPFMGTWVLGRNGTHRDALLDTALFAGGRIAAYATLG
                     AVAGGAGMWLSAMLRGGTGNAVIGMASILAGMWLLRPAQAHRPCAIVRTAGRTPPLLL
                     GFSLSLTPCAPLASLLAVCAATGNMGMGMANGVAFGLGAAFTPLLLLLPLICMFGKRL
                     RENRDWLARWVRWGAAAVLILLGLRRLLLAL"
BASE COUNT         2069 a         2775 c         2471 g         1803 t
ORIGIN      
        1 ggcaatggca gcggcttcgg cggcggtaat cgcagctcgg ccttcaatgg caacggcaac
       61 aacggcggtg caggttttca tcacagtgct ccggacagcc gtcatagcca cgcaggccac
      121 aaggaaggtc agagccacac cggtttcgcc ggacaagctc agccacgcag ctcggagcgc
      181 agcttcggca atgccaatcg cgcaccaagc agcggcgctc cgcgccgtag cggcggtggc
      241 gaccgtccag cgggtggaca gggccgcacg aataagtggt aagtatccag cggtaagaga
      301 atgaacaaag gcacgcctag gcgtgccttt gtttttttaa gggatcacaa ccttgcctga
      361 cgactcgtct tgctcgcaca tgatggactt tccggcatac aaaaaagccg atggatggat
      421 caaaaatcgc cagaccgttc ccacggaaac agcaacaagt ggtaaggtag catctgacga
      481 gaacgaagca tctgagcacc actgcaccat gcccaacata gccgaaattg ccgagagtca
      541 actggatgat ccggcctatc tgaaagccat caccgaactg ggagacagcg ttcctctcgt
      601 taccacccag aatctcaatt catcgaccgg aatcaaactg gtcaatgccg gaactcgctt
      661 caacagttcg ctctataaca aactcttgca gcacaagctg gtaccgacac tggatcaatc
      721 gctggccatc gaggacgcgg tcagcaacaa cagtctggcc gcagagctgc caacgatact
      781 cgctgaagaa ggatggctgc agcccatgct ggcaaccatg ttgaatcacg agatattcga
      841 acagatcctg accaagctgc cgctccatcc aaccgccgcc ttcaaactga cggtgatgcg
      901 agaaaggcat ccggggctgt tccggcgcag catctgcctc accttgattt gcatctatct
      961 gggcatccgt tgccagatgg attccaataa actggtccag ctcgccaccg cagctctgct
     1021 gcatgacatc ggcatgctgc acattgaccc agcgcttctg gcagagggtt acacgctaaa
     1081 caatgcggag cgtcgccacc tgtacgccca cccgctgacc gcctggatga tactgaaaaa
     1141 atgtaccgac tattcaccgg cggtcctcga tgcggtactg caacatcacg aacgtctgga
     1201 cggcagcggt tatccgcaag gactggcagg caacaacatc agcctgaacg gccaaatcat
     1261 cgcggtcgcc gaaatcatca ccagccgcaa tggcaacatg acctcggcat tcgacaagat
     1321 gcgcctggaa gccatgctaa agctcaattc cagacgctat ggacgccggc tcatcggcta
     1381 tttggatgtg ttttaccgca gcgacgaggc ggttcccgct tgcaccgatg cttacaagca
     1441 actcatcagg aaccaattgc tttgcacgca gtctgccttt tccgaatggg aaaagttgtg
     1501 ggatgcccac tatcaggggt atgccacttt tgactccatc aaccgacgca tgctcaacct
     1561 gaaaatggaa gtgctggatg ccgggctcgc cccactggat atcgacgaga gcctggatgg
     1621 gatcgaagac agtccacgcg cctgtttcga gatgcacatc ctgctggatg aggtgatatg
     1681 gcaattgcaa agcgtactgg atgagataag acggcgctgg cccgacatca acggcgaggg
     1741 cccggcttcc tgcgccatcc acatcctgaa ctggataaat gcaacagaac agaacatcaa
     1801 gtcgaataaa ctcacgaatc cagcaggcaa gagccgagga aagtgacgcc cctcgaccca
     1861 agagatcgca caacatgaaa cttgaacaag cccgacgatt gaccagcaga ttttcgaact
     1921 ggtttagcgc gcttcacccc cgcaatagcc tggttgccca gatcagctat gccatggcac
     1981 tggtatccat cgttctttcc gtggcgctca gctattacgc cgcgagcctc agtcgtcaac
     2041 agatcgaaca ggaactgggc cgcaactttg tacatcgcgc ccagaacatt gtggatgtgt
     2101 tggaccgagg catgttcgaa cgctaccgag aagtgcagat cgtcgcgaca ttgagcgaca
     2161 tacgcaatcc cgccgtcagc atcgatcaaa agcgtgtcgt gctggaaaag ctacagcaga
     2221 cctttgacgc ctatgcctgg gtgggaattt gcgacttgca gggcaagggg atagtcggca
     2281 caggcaaata tctcgaaggc aaggatctgt cgaaacgccc ctggtgcaaa caagggcgag
     2341 acaaacccta cgtcggcgat gtgcatgacg cgctgctact gtccaagata ttgcccaacc
     2401 caagcggtga gaacttctat ctggttgacg tcgcagcccc ggtcatcgac aaatccggtg
     2461 cgctgcaagg tgtgctgtgc gggcacattt actggaagtg ggccgaggaa ttgctcgaca
     2521 gcaagaagaa tgagtcattt gaaatcctgt tgctcagccg ggatggtctg gtattgaccg
     2581 ggccagagca aaacagaagc gaattaaaaa aactggcgcc tgactcctgg agccgcatca
     2641 tcaacggcaa acccggccaa gacaccctgc tggatcgatg gagcaacggc aagcaatatc
     2701 tggtcggcta tgcgcacgat gcgggctatc gcgactatcc gggactggga tggatcgcgc
     2761 tagtccgtca ggatgccgcc accgctttcg ctccggcacg caccctgcag ctgcgtatcc
     2821 tgcttgccgg cgtgatgctg ggcatcttct tcatcttctt cggagcctgg atggcaagac
     2881 gcatcgccac accgatctcg cgcatcacgg cggcggcgga aaaaattgcg gcgggcgaat
     2941 tcgaatatga cgcgcctcct gccgatggcg atggcgaagt cgcccatctg tccgcctcga
     3001 tacacaccat ggtgagcaat ctgacccgtg agatccttga ccgaaaaaaa gccgaggaac
     3061 aattgcgcct gtcggctgcg gtcttcgcca ataactcaga ggcgatcgtc atcaccgacg
     3121 cgcacaacga catcgtacgc gtcaacgatg ccttcacccg aatctcagga tatagcgcgg
     3181 atgaggtcat cggcctgaat ccccgcatct tctcttccgg caatatggac gagaagttct
     3241 accagaaaat gtgggccacc ttactggaga acaatgggtg gaccggcgag atatggaaca
     3301 aacgcaagaa tggcgaaatc tatccggagt ggatgatact gagcctggtg cgcgatgagg
     3361 ctggcaacat caccaatcac atcgccattt actcggacat caccgtacgc aaacaagaag
     3421 aggagcagat tcagtttctc gccaatcacg atgtgctgac tagcctgcct aaccgcaccc
     3481 tgctgaccga tcgtctcgaa caggcgctgg catttgccga acgcaacaag aacaaggtcg
     3541 ccgtactgtt tatcgacctc gatcacttca agaacatcaa cgattcgctc ggacacgacg
     3601 tcggcgacaa tctgctacgg caagcagccc tgcgcatcag cgcctgcttc cgtcgcaccg
     3661 ataccatcgc ccggctgggc ggcgacgagt tcgtcgccgt gctgccggag atcagctcgg
     3721 acaacgaagt gatatccctc gtcgaaaaaa tgctccatgc attcggcgag aagttcatgg
     3781 tcaacgaaca tcaactctcc atcaccccca gcatcggcat cagcctgttt ccggatgacg
     3841 gcctcaaacc ggtcaccctg ttgcgcaacg ccgatatcgc gatgtatcgg gcgaaagcac
     3901 aggggcggaa cacacttcag ttctaccgcc ccgagatgac ggcccatgcg accgagcgcc
     3961 tgcaactgga aatgcaattg cgccaggcca tcgacaacgg cgaactgttc ctgcactacc
     4021 aaccccaggt cgacctgaat agcggcgaca tgatcggtat ggaatcgctg attcgctgga
     4081 agcatccgca acagggcatg atctctcctg cacgctttat tccgattgcc gaggagagcg
     4141 gtctgattct ggagatcggc acatgggtgc tgcgcgaagc ctgcatgcag ggtcgcatct
     4201 ggcaaaagca aggctacaag gtcgttccga ttgcagtgaa cgtctccggc gtgcaattca
     4261 agcgcggcca gatcaccgag atagtgcaca acatcctgga tgagaccggt tttgacccac
     4321 atctgctcga cctggagatc actgaaagtg tgctgatggc ttccggcgaa gccagcctcg
     4381 acatcatgaa ttcgctcaag gcactgggcc tgacgctgtc gttagacgat ttcggcacag
     4441 gctattcctc gttgagccgc ctgaaatcac taccgctcga ctatctcaag gtcgatcaat
     4501 ccttcgtgcg cgatatcgcc accgacccca atgatgccgc catcgtacgt gccatattga
     4561 gcatgtcgca cgagatgaat ctgaaagtca tcgccgaagg cgtggagacc acggaacagc
     4621 tggaattcct gcgcagcctg aaatgcccga ccttccaggg ctacctgttc agtcgcccta
     4681 tcccacccga ggaaatcgag ttgtatctca aaacagcctg aactgtttca gatagcccgc
     4741 tctcctgaaa cgatgtcaga cggatacagc atggtgtgga gccatgctga tccgaagacc
     4801 tgctcagata tgcgccgttg cccatctcgc ctggtctatg ccccactttt cgaaggactc
     4861 atgagatttg atcttgtcat gcgacgcggc aagatcgacc gtctcaggac tcatgtggct
     4921 ggatttcttg cagtcgtaaa tcaattgcac gataggctgc aacaacctgt ccggcacgac
     4981 ctcgcgcacg attcccagct tatcgctctc catgcgcacc agtgatcccg ccggataaat
     5041 cccgatgccc ttgatgaagg cctgcaccag cttgggatta aagtgcgcgc cgctccactc
     5101 gtacattttc ttcaatgcct cggtcggcgg catgcccttg tgatagaccc gaatcgaggt
     5161 gatcgcgtca tagacatcga cgatagccag catttgacca tgcaggctga tctcatcgcc
     5221 tttcaatcca ttggggtaac ccgttccatc gtaacgctca tggtgttgtg cggccgcatt
     5281 gaaagcgatc gtactgatgc ctttgacctc cttgatcaac tgggccgtgt tcaccacatg
     5341 acttttcatc agcgcaaatt ccgcttcatc cagtttgccc ggcttgttca atacgcgccc
     5401 aggcacctga gccttgccta cgtcatggag caatcccccc atagccagct gcctgatttc
     5461 atcgtgcggc atatccaata cgcgcccgaa ggcgacagct aacgccgcga ccgaaaccga
     5521 atgctggaag gtgtattcgt cacgcgtctt gatctgagcc agcggcaaaa gtgcggaagg
     5581 gaagctgtac atcgactcga cgatgccgtc gatcatcggc tcacattgtt tcaaatcaac
     5641 ctgattccca agccgcacat cgcgcatgat ttcctgcatc agcttactgg cgttggagca
     5701 tacggctttg gcgtgattga acttaccaac aggcgacaaa ttggcgatga tcttcggcac
     5761 gaacgtggat tgctcgcaga caggcggcac aatctctgca cctgcctcga gctcggctga
     5821 acttccttcg ctatccatca acccggtcgc cctcacatcc agtcccagtt ccgtgtcgat
     5881 ataaagctcc gtcacacccg tctcgatgat cttgttaaag acggctgtat cgtcaatcaa
     5941 gaaccggttg cgagcgaatg ggtgatctat ccagccgcag ttaagatcat ggatgtacat
     6001 accaacaatg agttcgccga tggggatttt ttttatcatt tccgctcggc catttttact
     6061 gccgacgagg ccagcatcat catgcgcagt tgaccagggt ggcgatcagg caagttcatc
     6121 aggcttcttt gttccgacgc tcgcccgcct cttcggcatc gtgaaaacag tcatggcgcc
     6181 atgagcattc ggattgtttc ttgctgcacc caaccgtggc tggcgtggca tagcaggcga
     6241 cttgtccatt ttcagactgc atctggcgga tcatttcaac ttgaatgctg aacgattgat
     6301 tttcatttaa tcccacgatt tcctcgaccc gtttaagttt aattcgtccc cctgtcatag
     6361 cgacaatgag gtctgttctg caaagcgact cgcttgatag catcgtgacc aattgaaaca
     6421 gggacacaag gtatgagggc aggcgcggca gcttgagttc tggcagttgc aatcaatcgc
     6481 agactggcag ccccggtcga gatcggataa aaccatccac cccgttacgc ccttgtcttt
     6541 caacaaatat gccgtctggg tagagtattc aaatccatcg tggtaaacaa tatgcctaaa
     6601 tatatagaca tttaggccta ggacgagcct tagttcggct gacgggctgc ggcactgaaa
     6661 gcgctcctac cgcagaaaac aggcgatctg cggaggctgt tttttgactc ggggcaagat
     6721 ccgcctcaag gttgaatcta ttttgccagc gtgatgaacg atagcttggc gacaccggcg
     6781 tgctgcgcca acgccatcag ttcggcgata cgcccgtaag gcacggcgtt atcggcttcg
     6841 atctgcaact ggaattgcgg gtcggtggct cgttggtgca gcagctcggc cagccctgcg
     6901 tcgctgacat gcctgttatc cagctccaga ttgccctgcg catccaccgc caggcttacc
     6961 tgttgcggct tgtcgctctg ctgcaccgag tcggtcctgg gcagattgat cttgagggcc
     7021 tgcggagcga gcaagggtgc ggtgatgatg aacaccacca gcagcaccag catcacatcc
     7081 accagcggcg tgacgttgat ctcgctcatc atgttgtcgt cggaataatt gccgaaggcc
     7141 atcgccctac cccttgaagt gatgtttgag cgccagacgc atgaagtcgt gcgcgaagtt
     7201 gtccagctcg gtgatgcgta ccctcaggtt gcgcaaaaaa tagttgtagg ccagcacggc
     7261 aggcaaggcg gttgcgatgc cgatcgcggt cgcaaccagc gcctcgccga tggggccggc
     7321 gaccacatcc agcgaggcgg agccgctctg gccgatgttc tgcaaagcat gcatgatgcc
     7381 ccacaccgtg ccgaacaggc cgacgaaggg cgccgtcgaa ccgatggtgg ccagttcggc
     7441 cagaccgcgc tcgtgataac gctggaggtt ctgcacctgc tgacgcaatt ggcgctccag
     7501 cacttcctgc ggcgcaccgg tgtgcatcag atcctgacga tccgcgtcca gcgccttgag
     7561 ctcggcgaaa ccggactgtg ccagccgcgc cacgtccccc tctccgtttc cggccatctg
     7621 cgccgcagcc tgccagtcgc gcgcggccca gaaggcgccc agaaaaagac ggttacgctt
     7681 gcggtcctgt acttgccgcc gcaacttgaa cagcgcgatg gaccaggttg ccaccgacag
     7741 cagcagcaac agcagcagcg taccggcaac gataggggaa tcgctcgaaa acagatgggt
     7801 catacttcgt tttccttcag tgaaaattgt atcggaacct tgaaccattg cgtgacggga
     7861 tgcccagccc ggctcgccgg aacgaagtgc cagctcttta cggtgcgcaa tgcggcattg
     7921 tccagcacct catgcccgct gctctggaac acgctgactt cgccgcaatc ccctgccgat
     7981 cgcacctcga cgttcagcac caccctgccc tcccatccca tgcggcgtgc gaccatcgga
     8041 taggacggtc gcggattgtt gagatagctg gcccgatagt ccggcaccgt atcaaacaca
     8101 ggcgctgccg cgaccgacgg cgcggcagcc tgagctggtg gcgcagcctg gaccggcgtc
     8161 tcctgctgcg cgacggctcc cggggctttc tgctcggtct gtttcgggac cggtttttcg
     8221 ctgcgctcgc tatgcggccg tggtttcggc gggaccggtt cgggctgcgc ctgggcttgc
     8281 gccacctcgg cctgccggat cgcgaccgac accgacatct cgctgaccgc aacgcgggcc
     8341 cgttcgggct gcatcatcca tgccgatgcc aaaccaaggt gcaccgccac aaccagcgcg
     8401 atgacaatca cccgttcgct taaagcggca gcgggaaatg ggtcggtgcg gttcatagcg
     8461 acagcggtca tgcaaattct cgacgaggcg aagtgcgcat tctaatagct gttggctatc
     8521 cgcgctgacc cgatgcgccg cttcacagcg ccagcaagag gcggcgcagt cccagcagga
     8581 tcagcactgc cgccgcgccc cagcgcaccc agcgcgcgag ccagtcgcgg ttctcccgca
     8641 agcgcttgcc gaacatgcag atcagcggca gcagcaagag cagcggcgtg aacgccgcgc
     8701 ccagcccgaa cgccacccca ttcgccatcc ccatgcccat attgcccgtc gcagcacaga
     8761 ccgccagcag cgaagcgagc ggcgcacagg gcgtcaggct caacgagaaa cccagcaaca
     8821 acggtggcgt tctccctgca gtgcgcacga tggcgcaggg cctgtgtgcc tgcgccggac
     8881 gcaacagcca catccccgcc aggatcgatg ccataccgat caccgcattg ccggtgccgc
     8941 cgcgcagcat tgccgacagc cacattccgg ctccgcccgc caccgcaccc agcgtcgcat
     9001 aggctgcgat gcggccgcct gcgaacagcg ccgtgtcaag caacgcatcc cgatgggtgc
     9061 cgttgcgccc cagcacccag gtacccatga acggcaggca ggtagccgta caggcggt
//
LOCUS       DASUIV010000064         8546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_573998, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000064 DASUIV010000000
VERSION     DASUIV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573998"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1274)
                     /gene="cysM"
                     /locus_tag="VFW59_02345"
     CDS             complement(387..1274)
                     /gene="cysM"
                     /locus_tag="VFW59_02345"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEX5363083.1"
                     /translation="MYQTLEDFVGNTPLVRLKRIPGDTSNVILAKLEGNNPAGSVKDR
                     PALAMIARAEARGDIKPGDTLIEATSGNTGIALAMAAAMRGYKMILVMPENQSVERRQ
                     TMRAFGADLVLTAKEGSMELARDTAEQLRDAGRGIILDQFGNPDNPLAHYEGTAPEIW
                     RDTGGRITHFVSSMGTTGTLMGCARFFKEQNPAIQTIGVQPEEGAQIPGIRKWPEAYL
                     PKIYSPKNVDRLEYVSQSDAEEMTRRLASEEGIFCGISSGGAMSVALRISRQVENAVI
                     VSIVCDRGDRYLSTGVFPG"
     gene            complement(1371..2348)
                     /gene="cysK"
                     /locus_tag="VFW59_02350"
     CDS             complement(1371..2348)
                     /gene="cysK"
                     /locus_tag="VFW59_02350"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5363084.1"
                     /translation="MTNWYADNSLTIGHTPLVRLNRVTDGVNATVLAKIEGRNPAYSV
                     KCRIGAAMIWDAEKRGLLGAGKELVEPTSGNTGIALAFVAAARGIPITLTMPETMSTE
                     RRKLLLALGATLILTEGAKGMSGAIAKATEIAESDPSRYVLLQQFKNPANPAIHESTT
                     GPEIWNDTDGKVDIFVAGVGTGGTITGVTRYLKQTRGRAIRSVAVEPSSSPILTQKRA
                     GQELKPSPHKIQGIGAGFVPDILDLSLIDDIETVSNEDAVLYAQRLGREEGILAGISS
                     GAAVAAALRVARRPESAGKIIVVVLPDSSERYLSSVLFEGMFDSAGMPI"
     gene            complement(2502..3500)
                     /gene="rfaD"
                     /locus_tag="VFW59_02355"
     CDS             complement(2502..3500)
                     /gene="rfaD"
                     /locus_tag="VFW59_02355"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX5363085.1"
                     /translation="MYYIVTGAAGLIGSNLVKALNERGENNIIAVDNFKRADKFKNLV
                     DCDIADYLDKKEFLKRLQDGFFDGVVNAVFHEGACSDTMESDGHYMMENNYQYSLELL
                     NHCQNEEVPFLYASSASVYGGGSVFKESRENEAPLNVYAYSKFLFDQIVRRRWSKRTA
                     QIIGLRYFNVYGPREQHKGRMASVAFHFFNQYRAEGKVKLFEGCDGYANGGQLRDFVS
                     IEDVVKVNMFFLDNTHKSGIFNLGTGQAQSFNDVAVATLNTLREAEGKPALSHAELQQ
                     QGLLEYIPFPDALRGKYQSYTQADIGALRSVGYAEPFLTVEQGVSRYVQQLLKTAG"
     gene            complement(3544..4485)
                     /gene="rfaE1"
                     /locus_tag="VFW59_02360"
     CDS             complement(3544..4485)
                     /gene="rfaE1"
                     /locus_tag="VFW59_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX5363086.1"
                     /translation="MSVLPRFEGAKVLVVGDVMLDRYWFGDVARISPEAPVPVLKVTR
                     VEERPGGAANVARNIASLGAQVTLLSVVGADEAGDCLQRLLTEQGQVHALLQRDETIS
                     TTIKLRAVARQQQLLRIDFETPPSHEVLNAKLADFRKRLPDADVVVLSDYGKGGLTHI
                     AEMIRLARAAGKPVLVDPKGDDYARYRGASMLTPNRSEFREVAGNWKSEEDLTARAEA
                     LRRELELDALLVTRSEDGMSLYREGSVLHEPTQTREVFDVSGAGDTVIATLAVMLASG
                     AELPEAMRIANRAAGIVVGKLGTAVVTREEIVQDMGR"
     gene            complement(4485..5183)
                     /gene="pyrF"
                     /locus_tag="VFW59_02365"
     CDS             complement(4485..5183)
                     /gene="pyrF"
                     /locus_tag="VFW59_02365"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5363087.1"
                     /translation="MNDPKIIIALDYPQAAPALELADRLDPKLCRLKVGKELFTATGP
                     ALLEQLMRRGFDIFLDLKFHDIPNTTAQACKAAAGLGVWMVNVHALGGRRMLEAAREA
                     IANSAQQPKLIAVTMLTSMAQDDLADLGIAATPAEMVLRLATLARDSGLDGVVCSAQE
                     AALLRERCGSEFCLVTPGIRPAQASLDDQSRVMTPQEALKAGSSYLVIGRPITRAADP
                     LQALLAINQEIGAA"
     gene            complement(5198..6373)
                     /gene="lapB"
                     /locus_tag="VFW59_02370"
     CDS             complement(5198..6373)
                     /gene="lapB"
                     /locus_tag="VFW59_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEX5363088.1"
                     /translation="MEFEPWMLLIFPLFFGMGWLAARIDIKDLLSESKALPHSYFQGL
                     NFLLNEQQDKAIEAFIEVVKVDPQTVELHFALGSLFRRRGEVDRALRMHHNLLDRADL
                     DTDKRQHAIFELAQDYLKAGIFDRAESLFGELEKSSYAKPALEFLLELFQKEHDWLKA
                     IDVAQRLTQVSGQSYSKHASFFYCELANEDLAAGDAEAAREHLLQALSTCPNAVRASM
                     MLGDMALVDGHLEEAIAVWKHIEVQDAQYLPLVAERLLNAFRSLDRETEGIALLRDYL
                     DKYHSLDLMNVLFDGVLKIEGSSAAYQLVRSELQRNPSLLGLDKLLEARLLEVPMDRR
                     ADVELVKDLIHKRTRNLATYHCSHCGFKARQFYWHCPACQSWDSYLPRRTEETGIPL"
     gene            complement(6396..6692)
                     /locus_tag="VFW59_02375"
     CDS             complement(6396..6692)
                     /locus_tag="VFW59_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX5363089.1"
                     /translation="MHYLNWFLRAVLFVLLLGFAVKNDQPVSLRYFFGYEWQSSLVVV
                     LLLFFAAGAAIGVLAMLPNVLQQRREIARLKREIRVKNTIAGVGETQQTPIQPS"
     gene            complement(6784..7071)
                     /locus_tag="VFW59_02380"
     CDS             complement(6784..7071)
                     /locus_tag="VFW59_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX5363090.1"
                     /translation="MTRSDLIAKLAELHPQLLAKDAELAVKVILDTLSSTLGRGGRVE
                     IRGFGSFGLNYRPPRQGRNPKTGDKVKVPAKYVPHFKAGKELRERVCEPKA"
     gene            complement(7086..>8546)
                     /gene="rpsA"
                     /locus_tag="VFW59_02385"
     CDS             complement(7086..>8546)
                     /gene="rpsA"
                     /locus_tag="VFW59_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX5363091.1"
                     /translation="NGYGETKLSREKAKRLAAWHDLELAMEEGKVVEGFVSGKVKGGL
                     TAMVNGIRAFLPGSLVDIRPVKDTTPYENKLMELKVIKLDRKRNNVVVSRRAVLEETM
                     GADREAIMENLKEGAIVKGVVKNITDYGAFVDLGGIDGLLHITDLAWRRVKHPSEVLN
                     VGDEVEAKILKFDQEKNRVSLGIKQMGDDPWNGLARRYPQGTRMFGKVTNLTDYGSFV
                     EIEQGIEGLVHVSEMDWTNKNVHPSKVVSLGDEVEVMILEIDEQRRRISLGMKQCQSN
                     PWDDFAANHKKGDKVRGAIKSITDFGVFIGLDGGIDGLVHLSDLSWNQPGEEAVRNYK
                     KGDEIEAVVLAIDVERERISLGIKQMEGDPFGGFVTGNEKGAVVTGVVKSLDAKGATV
                     MLDGDVEAYLKASEVSADRVEDIRNHLKEGDSVTAVIINVDRKNRGINLSIKALNKAE
                     ETAAMQKFSAENTGTAGTTNLGALLKAKMSGSKTEA"
BASE COUNT         1740 a         2714 c         2267 g         1825 t
ORIGIN      
        1 ttcccaaaaa ccaagtacct acactcgtcg gttgtttgtt ttaactttta aagagcggct
       61 gccgtagcag cgaagaggtg cgcattatag agactaaaat cttactgtca actttaattt
      121 cacactcttt tcatctgcca ctatagcgag gagaacgccg tcatttactg acagttaagc
      181 gaaattaaat gcagttcact ttgccttcac cccgccacag cgaagagggg cgcattatag
      241 ggagccagac ttttctgtca acaaaatatg gaggtttatt tcctgcccac ccgcccggcg
      301 atttccgaaa gccatgtttt tgccgttatg tcccatgatt ccatgcaaaa ttcccgactc
      361 agatcatttg catcagcttt tctgcatcat cctggaaata cccccgtgct cagatagcga
      421 tcgccacggt cgcacacgat cgagacgatg accgcattct cgacctgccg ggatatccgc
      481 aatgcgacac tcatcgcccc gccggatgaa attccacaga agataccctc ctcgcttgcc
      541 agtcgccgcg tcatttcttc cgcatctgac tggctcacgt attccagcct gtccacattc
      601 ttcggactgt atatcttcgg cagataggct tcgggccact tacggatacc tggaatttgc
      661 gcgccctctt ccggctgcac cccgattgtc tggatagccg gattctgctc cttgaagaag
      721 cgcgcacagc ccatcagtgt gccggtcgta cccatgctgc tgacgaagtg agtgatgcgc
      781 cccccggtgt cgcgccatat ctccggcgca gtgccttcat agtgcgcgag cggattatcc
      841 ggattgccga actggtcgag gatgatgccg cgccctgcat cgcgcagttg ctctgccgtg
      901 tcgcgcgcca gctccatgct gccctccttt gcggtcagca ccagatccgc cccgaatgcg
      961 cgcatggtct ggcggcgctc cacgctctga ttctccggca tcaccaatat catcttgtag
     1021 ccgcgcatcg ccgccgccat cgccagcgcg atgccggtgt tacccgaggt cgcctcgatc
     1081 agcgtgtcgc ccggcttgat gtcgccgcgc gcttcggctc gggcaatcat agccagcgcg
     1141 ggcctgtcct tcaccgaccc cgcaggattg tttccctcca gtttggcaag gatcacgttg
     1201 gaggtatcgc cgggaatacg tttaaggcgc accagaggtg tattgccgac aaagtcttcg
     1261 agagtttggt acatcgctca accttaaaat ttcagaactg cgccatgata actggcattc
     1321 aaacagaaca gggagcctca gctccccgtt ctgtttacgc cgcgcttacg ctagatcggc
     1381 atgccggccg aatcgaacat gccctcgaac agcaccgagc tcaggtagcg ttcgctggag
     1441 tcaggcaaca cgaccacgat gatcttgcca gcactctccg ggcgcctggc cacccgcagc
     1501 gcggccgcta ccgccgcacc ggacgagatt cccgcgagta tgccctcttc cctgcccaga
     1561 cgttgcgcat acaacaccgc atcttcattg ctgacggttt cgatatcgtc gatcagcgac
     1621 aggtcaagaa tatccggcac gaagcccgcc ccgatgccct gtatcttgtg cggcgagggt
     1681 ttcagttcct gcccggcgcg tttctgggtc aggatagggc tggacgacgg ctcgactgcg
     1741 accgagcgta tcgcgcgccc cctggtctgt ttcaaatagc gcgtaacgcc ggtgatggtg
     1801 ccgcccgtgc cgacaccggc gacgaatatg tccaccttgc cgtcggtatc gttccatatc
     1861 tccggcccgg tggtgctttc gtggatcgcc gggttggccg gattcttgaa ctgctgcagc
     1921 aacacgtaac gagagggatc ggactcggcg atctcggtgg ccttcgcgat cgcgccgctc
     1981 atgcccttgg caccttcggt caggatcagc gtcgcgccca gcgcgagcaa cagcttgcgc
     2041 cgctcggtgc tcatcgtctc gggcatggtc agcgtaatgg gaatgccgcg tgccgccgcg
     2101 acgaacgcaa gtgcgatgcc ggtgttgccg gaagtcggct cgaccagttc cttgcccgca
     2161 ccgagcagac cgcgcttctc ggcatcccag atcatcgccg cgccgatacg gcacttcacc
     2221 gagtaagccg ggttgcggcc ttcaatcttg gccagcacgg tggcgttcac gccgtccgtc
     2281 acacgattca gacgcaccag cggcgtgtgt ccgatagtca gcgaattgtc tgcataccag
     2341 ttagtcatgc tctagtcctt tcgagagtga tcgtcagttc ggaggcaact ataggggaca
     2401 acccttattc cgcaaaatgc tgatttgtta tttcgttatg caatttaagc agatataaaa
     2461 aagcggcaga catcgagtct gccgccggaa aagacttttc cttatcctgc cgttttcagc
     2521 aactgctgca catagcgcga cacgccctgt tccaccgtca ggaagggctc ggcatagccg
     2581 acgctgcgca atgcgccgat atccgcctgg gtgtagctct gatacttgcc acgcaatgca
     2641 tcgggaaacg gaatatattc cagcagcccc tgctgttgca gctcggcatg gctcaatgcg
     2701 ggcttgcctt cagcctcgcg caaggtgttg agcgtggcga cagccacatc gttgaagctc
     2761 tgcgcctgcc cggtgccgag gttgaaaatg ccggacttgt gcgtattgtc gaggaagaac
     2821 atgttgacct tgaccacatc ctctatcgag acgaagtcgc gcaactgacc gccgttggca
     2881 taaccgtcgc agccttcgaa cagcttcacc ttgccttcgg cgcgatactg gttgaagaag
     2941 tgaaaagcca ccgaagccat gcgtcccttg tgctgctcgc gcgggccata cacgttgaaa
     3001 taacgcaggc cgatgatctg cgcggtgcgc ttgctccagc gacggcgcac gatctggtcg
     3061 aacagaaact tggagtaggc atatacgttg agcggcgcct cgttctcgcg gctctccttg
     3121 aacaccgaac cgccgccata taccgaggcc gacgaggcgt agaggaacgg gacctcttcg
     3181 ttctggcagt ggttcaacag ctccagcgag tactggtagt tgttctccat catgtagtgc
     3241 ccgtcgctct ccatcgtgtc cgaacaggca ccctcatgga ataccgcatt caccacaccg
     3301 tcgaagaagc cgtcctgcag acgcttcaga aactctttct tgtccagata gtccgcgatg
     3361 tcgcaatcca ccagattctt gaacttgtcg gcgcgcttga agttatccac cgcgatgatg
     3421 ttgttttcgc cgcgctcgtt gagcgccttg accaagttag aaccgattaa cccggctgcg
     3481 ccggtaacga tgtagtacat gttttctcct ttaatgaacg taggggcacg gcgcgccgtg
     3541 cccctaccgc cccatatcct gaacgatctc ttcgcgggtc accaccgccg tacccagctt
     3601 gccgactacg ataccggccg cgcggttagc gatgcgcatc gcctccggca actcagcgcc
     3661 ggaggccagc atcaccgcca gcgtggcgat cacggtgtca cccgcgccgc tgacgtcgaa
     3721 cacttcgcgt gtctgcgtcg gctcgtgcag cacgctgcct tcgcgataca gactcatgcc
     3781 gtcttcgctg cgcgtgacca gcagcgcgtc cagttccagt tcgcggcgca aggcctcggc
     3841 gcgcgcggtg agatcttcct cactcttcca gttgcctgcc acctcgcgaa actcgctgcg
     3901 gttcggcgtg agcatcgaag cgccgcgata acgcgcgtaa tcgtccccct tgggatcgac
     3961 cagcaccggc tttcctgccg cgcgcgccag ccggatcatc tcggcgatat gggtcagccc
     4021 tcccttgccg taatcggaca gcaccacgac atcggcatcc ggcaagcgct tgcggaagtc
     4081 cgccagtttc gcgttcagca cctcgtggct gggcggcgtc tcgaagtcga tgcgcaacaa
     4141 ctgctgctgg cgcgcgaccg cgcgcaactt gatcgtggtg gaaatagtct catcgcgctg
     4201 caatagcgca tgcacctgcc cttgctcggt cagcagccgt tgcagacaat cgcccgcttc
     4261 gtccgcaccg accaccgaca gcaacgtgac ctgcgcaccc agcgaggcga tgttgcgcgc
     4321 cacgttggcc gcaccgccgg ggcgctcctc aacgcgagtc accttcaaca cgggcacggg
     4381 cgcttccggc gagatacgtg cgacatcgcc gaaccagtaa cgatccagca tcacatcgcc
     4441 aaccaccaac accttcgccc cctcaaacct tggtaataca ctcatcacgc agctccgatt
     4501 tcctgattga tcgccaacaa tgcctgcagc ggatcggcgg ccctggtgat ggggcgaccg
     4561 atcaccagat agctggagcc cgccttgagc gcttcctgcg gcgtcatcac gcgcgactgg
     4621 tcgtccaggc tggcctgtgc cggacggata cccggggtca ccagacaaaa ctcgcttcca
     4681 cagcgctcgc gcagcagtgc cgcttcctgc gccgagcaca ccacaccgtc cagtccgctg
     4741 tcacgtgcga gcgtggccaa gcgcaacacc atctcggcag gcgtggccgc gatgccgaga
     4801 tcggcgagat cgtcctgcgc catgctggtg agcatggtca ccgcgatcaa cttcggctgc
     4861 tgcgcactgt tggcgatcgc ctcgcgtgcc gcttccagca tccggcgtcc gcccagcgca
     4921 tgcacgttca ccatccacac ccccaggccg gcggcagcct tgcaggcctg cgcggtagtg
     4981 ttggggatgt cgtggaattt caggtcgaga aagatatcga aaccgcgccg catcagctgc
     5041 tccagcaaag ccgggccggt ggcggtaaac agctccttgc ccaccttcag acggcacaac
     5101 tttggatcaa gtcggtcggc cagctccagc gccggggctg cctgaggata gtccagcgcg
     5161 atgataattt tggggtcgtt catgctcttg gatacgttca taaaggtatt cctgtttctt
     5221 cggtacggcg cggcaagtaa ctgtcccacg actggcaggc cgggcaatgc cagtagaatt
     5281 gacgcgcctt gaagccgcaa tggctgcaat gataggtcgc cagattgcgc gtgcgtttat
     5341 gaatcaggtc tttcaccagt tcgacatcgg cacgcctgtc catcggcact tccagcaacc
     5401 gcgcctcaag caacttgtcc agcccaagca gtgaaggatt gcgctgcaat tcgctgcgca
     5461 ccagctgata ggccgcagac gagccctcga tcttcagcac accgtcgaac agcacattca
     5521 tcagatccag cgagtggtat ttgtccagat aatcgcgcag cagggcgatg ccttcggtct
     5581 cgcgatcgag actgcgaaac gcattcagca gccgttccgc gaccagcggc agatattgcg
     5641 catcctgcac ttcgatgtgc ttccagacgg cgatggcctc ttcaagatgc ccatcgacca
     5701 gagccatatc gccaagcatc atgctggcgc gcaccgcatt cggacaggtc gagagcgcct
     5761 gcaacaaatg ttcgcgcgcc gcttcggcat cacccgccgc caaatcctcg ttcgccagtt
     5821 cgcaatagaa aaacgaagca tgcttgctgt aggactgacc ggacacttgc gtcagacgtt
     5881 gcgcaacgtc gatggccttg agccagtcat gctctttctg gaacagttcc agcaaaaatt
     5941 ccagcgccgg tttcgcatag gaactcttct ccagttcacc gaacagactc tccgcacgat
     6001 cgaagatgcc cgccttcaag taatcttgag cgagctcgaa gatggcatgc tgacgtttgt
     6061 cggtgtccag atcggcacgg tccagcaagt tatggtgcat gcgcaaggcc cgatccactt
     6121 cgccgcgacg gcggaacagg ctgcccagcg caaagtgcaa ctcgacggtc tgcgggtcga
     6181 ccttgaccac ttcgatgaat gcctcgatgg ccttgtcctg ctgctcgttg agcaggaagt
     6241 tcagaccctg aaaatacgaa tgcggtagcg ccttcgattc cgagagcaga tccttgatat
     6301 caatacgagc cgccagccat cccattccga agaacaaagg gaagatcagc agcatccacg
     6361 gttcgaattc cataatcgtc agaggtgtag cggcatcagg aaggttggat cggagtctgc
     6421 tgcgtctcac ccacaccggc aatcgtgttc ttaacgcgga tctcgcgctt cagacgcgca
     6481 atctcacggc gctgttgcaa tacattgggc agcatcgcga gcacacctat cgcagctccc
     6541 gcagcgaaga acagcaacaa tacaacgacc agcgaagact gccactcata gccaaaaaaa
     6601 tagcgcaacg acaccggctg atcgttcttg accgcaaaac caagcaacaa gacgaacaat
     6661 accgcacgca aaaaccaatt gagataatgc atagtttcca ccctgaacaa gcgccgcaac
     6721 gatagcataa aaaatggcgg ccataccttg cgatatgccg ccattcagat tactgcaagc
     6781 caatcaagcc ttaggttcgc agacccgctc tctcaactcc ttgcccgcct tgaagtgagg
     6841 cacatacttg gccggcactt tcaccttgtc gccagtcttg ggattgcgcc cttgacgcgg
     6901 cggacggtaa ttcagaccga agctgccaaa accgcgaatc tcgacacgcc ccccacgacc
     6961 gagcgtggaa gagagcgtat ccagaatcac cttgacggca agttcggcgt ccttggccag
     7021 cagctgcgga tgcagctcgg ccagtttcgc aatgagatca gaacgggtca tgcagtttcc
     7081 ttaaatcagg cttcagtctt gctaccgctc atcttggcct tcagcaaagc acccagattc
     7141 gtggtgccgg cagtgcccgt gttttcagcg gagaatttct gcatcgctgc agtttcttca
     7201 gccttgttca gcgccttgat cgacaggttg ataccgcgat tcttgcgatc cacgttgatg
     7261 atgactgcag tgacgctgtc gccttctttc aggtggttgc ggatatcttc gacgcggtca
     7321 gcggaaactt cggatgcctt caggtaggct tctacatcgc cgtccagcat cacagttgcg
     7381 cccttggcat ccagtgattt aaccacaccg gtcaccaccg cacccttttc attgccggtc
     7441 acgaaaccac cgaacggatc accttccatc tgcttgatac cgagggagat gcgctcacgc
     7501 tcgacatcga tcgccagaac aacggcttcg atctcgtcac ccttcttgta gttgcgcaca
     7561 gcctcttcgc caggctggtt ccacgacaga tcggacagat gcaccagacc gtcgataccg
     7621 ccgtccaggc cgataaacac gccgaagtcg gtgatggatt tgatcgcacc acggactttg
     7681 tcgcccttct tgtggttcgc tgcgaagtca tcccaaggat tggactggca ctgtttcatg
     7741 cccagcgaga tacggcgacg ctgctcgtcg atttccagga tcatcacttc tacttcgtcg
     7801 cccagagaga cgactttgga aggatgcacg ttcttgttgg tccagtccat ttcggaaacg
     7861 tgaaccagac cttcgatgcc ctgctcgatc tcaacgaacg aaccgtagtc ggtcaggttg
     7921 gtcaccttgc cgaacatgcg tgtaccttgt gggtaacgac gtgccagacc attccatgga
     7981 tcgtcgccca tctgcttgat acccagcgag acgcgattct tctcctggtc gaatttcagg
     8041 atcttggctt cgacttcgtc gcctacattc agcacctcgg atgggtgctt gacgcgacgc
     8101 catgccagat cggtgatgtg caacagaccg tcgataccgc ccaggtcaac gaacgcgccg
     8161 tagtcggtga tgttcttgac cacgcccttg acgatcgcgc cttccttgag gttttccatg
     8221 atcgcttcac gatcagcgcc catggtctct tccagtactg cacgacggga aaccacgacg
     8281 ttgttgcgct tgcgatccag cttgatgacc ttcagctcca tcagcttgtt ttcgtatgga
     8341 gtggtgtcct tgaccggacg gatgtcaacc agcgaacccg gcaggaacgc gcggatgccg
     8401 ttaaccatcg ctgtcagacc gcccttgacc ttgccgctaa cgaagccttc aacgaccttg
     8461 ccttcttcca tggccagttc cagatcgtgc catgcagcca gacgcttggc cttctcgcgc
     8521 gacagttttg tctcgccata gccgtt
//
LOCUS       DASUIV010000065         9086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_574159, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000065 DASUIV010000000
VERSION     DASUIV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574159"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..1372
                     /gene="tsaD"
                     /locus_tag="VFW59_02390"
     CDS             362..1372
                     /gene="tsaD"
                     /locus_tag="VFW59_02390"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5363092.1"
                     /translation="MITLGIESSCDETGIALYQMGHGLLAHALHTQIAMHSEYGGVVP
                     ELASRDHVQRVIPLVRQTLHEANISLEQVDAIAYTQGPGLGGALLVGASVANALAYAL
                     DIPTIGIHHLEGHLLSPLLSDPAPEFPFVALLVSGGHTQLMRVDGVGRYTLLGETLDD
                     AAGEAFDKSAKLLGLGYPGGPALAKLASQGNADRFKLPRPMLHSGDLDFSFSGLKTAV
                     LTLTKQHELDDQTRADIACATQEAIVDVLALKARAALAQSGLSQLVVAGGVGANRLLR
                     ERLSADIGKRGGEVFYPDLEFCTDNGAMIAFAGALRLAQQQGRKDYSFDVKPRWDLQQ
                     IA"
     gene            complement(1389..1991)
                     /gene="plsY"
                     /locus_tag="VFW59_02395"
     CDS             complement(1389..1991)
                     /gene="plsY"
                     /locus_tag="VFW59_02395"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX5363093.1"
                     /translation="MLTIAFVFAAYLLGSVSFAVLMSKAFGLADPRTYGSGNPGATNV
                     LRSGKKAAAALTLLGDAAKGWVAVVLAMSVAPHDAEGLLLIALVALAVFLGHLFPIFL
                     RFKGGKGVATALGVLLALNLWVGLGALVTWLVMALVFRLSSLSALVAAIAAPIYAIVL
                     GLPREWVLAAGIMSLLLIWRHKSNIQNLLTGKESKIGGKK"
     gene            2065..2421
                     /gene="folB"
                     /locus_tag="VFW59_02400"
     CDS             2065..2421
                     /gene="folB"
                     /locus_tag="VFW59_02400"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5363094.1"
                     /translation="MDIIFLRELKVETLIGVYEWEKRIPQTLQIDLDIALPSSRACAT
                     DDIADALNYADIVRHIQDVLASRHFHLLEALAEHIAQILLNDFKAPWAKVSVAKLQAI
                     RGSKMVGICIERGQAK"
     gene            complement(2463..3029)
                     /locus_tag="VFW59_02405"
     CDS             complement(2463..3029)
                     /locus_tag="VFW59_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363095.1"
                     /translation="MRFDQNPYNQILTASPSHGGDDFAAAESEVLLNESAAEVDVDRI
                     IASINSSQVEVEYIPGRCFTAIFHGAELLPFNKLLNLDHRTGELHRYRAACLKAAERA
                     VYAAEQLFECRRPELSERISVSLYRAGVKAFDNDELIAAYKLLIDGFREVGLLKENDP
                     SHISSFDCLLQRCGVNEAVSIGITFRRA"
     gene            complement(3150..3746)
                     /locus_tag="VFW59_02410"
     CDS             complement(3150..3746)
                     /locus_tag="VFW59_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363096.1"
                     /translation="MMALSDIVGLSGMAFALAVLIVPLVAARVTGRAVLLYIVVCMLL
                     ALSPFDGLSVAGYLRGFFGDLSVASLLLLVLALRRQVSGAEAEGRMGLLALIALAALA
                     FYPFALGIGLFDPYRLGFGHFGFLAALLLVALLAWMRQQVLIALYIALAVLAWAIGWG
                     ESNNLWDYLLDPWVAFYALGALLKRSVRLLRGAPVSAR"
     gene            complement(3743..5029)
                     /locus_tag="VFW59_02415"
     CDS             complement(3743..5029)
                     /locus_tag="VFW59_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5363097.1"
                     /translation="MFDRRVLFGIILIAFAAAFYKAANVPAVAAFQYRPAVVANVAAA
                     RFDSRFASSRFQTQVHAASLIELKDRSLRGFWFSGSREGAADVTINSALFDPVTGEWG
                     AEQSIASRDSTQQGLHRYIGKVGNPVPMRAADGRLWLFYVTVSLGGWAGSSITAITST
                     DEGVTWSEPRRLISSPFMNISTLVKAAPFLYADGTIGLPVYHEFISKFAEILRLDQSG
                     KVIDKQRLAAGGQGTLQPVVLVRSAQQALVLTRYSGNESPHRAKMMTTVDGGAHWSAA
                     VQSPLSNPDAALAALVLADGRLLAVLNDQQSGRDNLALMLSADGGVSWRRVAVLENET
                     ATSVQDEAACLSRVAALVKQSDARAGVSAVSLERYVDSAKAKVRAGDACSFEFSYPYL
                     MQASGGDFHLLYTWNRVFIKHLSFNQAWLEQQETQP"
     gene            complement(5029..6744)
                     /locus_tag="VFW59_02420"
     CDS             complement(5029..6744)
                     /locus_tag="VFW59_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX5363098.1"
                     /translation="MKTSRLIWYVLAMLLAVFTYFYELGSPNIPKNGDEFPYTHITRL
                     TAASGHLLPLQSELDHMRNTKPPLLFWQGIASTHWGSAWSWWQLRYPSVIYTLLTALL
                     VFLLAKRLAQQQKIEGDASPMETGFIAALTFLAFFSTYRFGRPFLVNPAEVFWFFLPF
                     CTLLYRRAFAFESPLFVPLLLGVEIGIGLLYKSFALVVPVGFALAWWYLHMRDYRLMA
                     FLLKDSWKVALTVTVALAMFGLWFLFDPNPQAVWQEFVVGENAGKFDPHGAGYFATLL
                     WGGSSIWALLLGYPINAGLLAFPVAAVFLVAWRRRTLLSNAERMLWIWLFALFLVFSV
                     PSQRSARYLLEAMPALAVLCALNWQRIGRPFFVASLLLSGAVLALLIFLSVRLQHEVG
                     DLYSILYWLLLLLTGGLILFGLFVARHTRMVMNGAALLVMLSLAAFLSPFDGAVGNFS
                     AEAQRYAKGKEVWTPCNFRAVDEGYRFLLPGARVHGYMAVPSPSVAELSANYGLFAVQ
                     LPLHQQCVGCRVVGQRLEIRGRHSPEEIRRIFSGEVFQQLFVQELLVEAPNAAPAQPP
                     KEGCR"
     gene            complement(6748..7239)
                     /locus_tag="VFW59_02425"
     CDS             complement(6748..7239)
                     /locus_tag="VFW59_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX5363099.1"
                     /translation="MRLGLNLRITLPSLLRRIFSRRFLKFGTVGASGTVVNIGVLYFA
                     QEHIFNAVQQPEIRLNLSLVLAIFFATLNNFFWNRLWTWSDRVQHHHRPWLAQFGQYT
                     LACWLSIALQLVFTNLLAPHFYYQIANLIAIGLTSVLNFVLNDIWTFGRVKLLVKSDK
                     DAG"
     gene            complement(7236..8456)
                     /locus_tag="VFW59_02430"
     CDS             complement(7236..8456)
                     /locus_tag="VFW59_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5363100.1"
                     /translation="MNIKFQRAVDRLAGVPICALLSVFERARRMLAGTAQRTAQPKRI
                     LVILLSEMGSLVLAQPMFAKLKQQYPDASLHILMFAKNREVLDLLGVMPPEHVITISD
                     KSLGGFVSDAWNALLTLRRLKCDVVIDCELFSRVSSLFAYFSGASLHVGFHPHTQEGL
                     YRGSFINRPVLYNPYRHLSQQFLTMVDAIASSGVPLAKGEAAELSAPPLLQFSDGELQ
                     QVGDGLHADFPVLAGKRLVLVYPDGGILPIRAWPPEYYKQMCAALLQEGYAVALIGLP
                     DAKPLAQQIVAHCAHPHCIDLTGYTKSVRHLLAIFNHASLLITNDGGPGQFAALTRLP
                     TLVFFGPETPALYSPLAPNIHCLYTALSCSPCLTAYNHRNSPCDGDNQCLKRITPDRV
                     LHKVRELLQGGAGA"
     gene            complement(8555..>9086)
                     /locus_tag="VFW59_02435"
     CDS             complement(8555..>9086)
                     /locus_tag="VFW59_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX5363101.1"
                     /translation="LAAPDVRTPLGLRDRTMFEVLYATGLRVSELVTLRITQVSMDMG
                     VVRVMGKGSKERLVPLGEEALDWLRRYMTDGRAVLLSGKVSDALFVTARGEGMTRQMF
                     WYLIKKHAKAGGLHKSLSPHTLRHAFATHLLNHGADLRVVQMLLGHADISTTQIYTHV
                     ARERLKRLHAQHHPRG"
BASE COUNT         2122 a         2936 c         2479 g         1549 t
ORIGIN      
        1 ttcccaaaaa ccaagtacct acactcgtcg gttgtttgtt ttaactttta aagagcggct
       61 gccgtagcag cgaagaggtg cgcattatag agactaaaat cttactgtca actttaattt
      121 cacactcttt tcatctgcca ccatggcggg caaaacgtca gcatactcct gatttaaaaa
      181 caagtttttc ttcaaacctg cctctcgttt caaccccgcc gcagcgaaga ggtgcgcatt
      241 atagggagtc agattttccc gtcaacacct ttctgtcaca tttctgtaat taattcgcag
      301 ccgccctact caatctcgtt attcactaca atgcagcctt gtctgaaagg ctgaaatgtc
      361 attgattaca cttggaattg aatcttcctg cgatgaaacc ggcatcgcgc tctatcaaat
      421 ggggcacggc ttgcttgcgc acgcactgca cacgcagatc gccatgcaca gcgaatatgg
      481 cggcgtggta cccgaactcg cctctcgcga ccacgtacag cgcgttatcc ctttggtgcg
      541 tcagacactg catgaagcca acatttcgct ggagcaggtc gacgctatcg cctacaccca
      601 agggccgggg ttaggtggcg cgctcctggt cggcgccagc gtggccaatg ccctggccta
      661 tgcgctcgac atccccacca tcggcatcca ccatcttgaa ggccatctgc tctccccctt
      721 gttatccgat cctgcaccgg agtttccctt cgtcgcatta ttggtctcgg gcggccatac
      781 ccagttgatg cgcgtagacg gcgtcggacg ctacacctta ttgggggaga cgctggacga
      841 tgccgccggc gaggccttcg acaagagcgc caagctgctc ggcctcggct atcccggcgg
      901 cccagcactg gcgaagctgg cctcacaagg taatgccgat cgattcaagc tgccgcgccc
      961 gatgctgcat agcggcgatc tggatttcag cttcagcgga ctgaagacgg cagtactgac
     1021 cctgaccaag caacatgaac tcgacgacca gacccgcgcc gatatcgcct gcgcaaccca
     1081 agaggctatc gtagacgtgc tggcactcaa ggccagagcc gcgctggcgc aaagcggact
     1141 gagtcaattg gtggttgcag gcggagtagg ggctaacaga ctgttgcgtg agcgactgag
     1201 cgcggacatc ggcaaacgcg gcggcgaggt gttctatccc gacttggaat tctgcaccga
     1261 taacggcgcg atgatcgcct ttgccggtgc gctgaggctt gcgcagcaac aaggccgcaa
     1321 ggattacagc ttcgatgtaa agccgcgctg ggacttgcag cagatcgcct gatccaggcc
     1381 cggcagattt acttcttgcc gccgatcttg ctttccttcc ccgtcagcag gttctgaata
     1441 ttgctcttgt gccgccagat caacagcagc gacatgatcc cagctgccag cacccactca
     1501 cgcggcagcc ccagcacaat cgcgtagatc ggtgccgcaa tggcggccac cagcgccgac
     1561 agcgaggaca ggcggaacac cagcgccatc accagccaag ttaccaaggc acccaagccc
     1621 acccagagat tcagtgccag caacacaccc agcgccgtcg ccaccccctt gccgcccttg
     1681 aagcgcagga atatcgggaa caggtgaccg agaaacaccg ccagcgccac caatgcgatc
     1741 agcagcagcc cctcggcatc gtgcggcgca accgacatcg ccagcacgac cgcgacccaa
     1801 cccttggccg catcgcccag cagcgtcagc gccgccgccg ctttcttgcc gctacgcaac
     1861 acattggtcg cgccgggatt gccggaaccg taggtgcgcg gatcggccag accgaaggcc
     1921 ttgctcatca acaccgcaaa agaaaccgag cccagcagat aggcagcaaa cacaaaagct
     1981 atggtcaaca tcggtaatgc cttagaattc aacgggaggc gaattctacg cgaatcgcca
     2041 cttgctcacc aaacatcgaa aatcatggat atcatttttc tgcgcgaact caaggtcgaa
     2101 accctcatcg gcgtctacga atgggagaag cgcatcccgc agaccctgca aatcgatctg
     2161 gacattgccc tgcccagcag ccgcgcctgc gcaaccgacg acatagccga cgcactcaac
     2221 tacgccgaca tcgtgcgcca catccaggat gtcctcgcca gccgccactt tcacctgctc
     2281 gaagccttgg ccgaacacat cgcccagatc ctgctcaacg acttcaaagc cccctgggcc
     2341 aaggtcagcg tcgccaagct acaggcgata cgcggcagca agatggtggg gatctgtatc
     2401 gaacgcggcc aggcaaaata accattacca cggagaattg cagggcaagc cgacaacatc
     2461 cgctaagccc gccggaaagt gatccctatc gataccgctt cgttgactcc acagcgctgc
     2521 agcagacaat cgaaagatga gatgtgcgat gggtcatttt ccttcagcag acccacctcc
     2581 ctgaatccgt ctatcagcaa cttataggcc gcaatcaatt catcgttgtc gaacgccttt
     2641 acacctgcgc ggtagagcga aacggatata cgctccgaca attccggccg ccgacattcg
     2701 aagagctgct ctgccgcata gacagcgcgc tcggcagcct ttagacaagc ggcacgataa
     2761 cgatgcaact ctccggtgcg atgatccaga ttcagcaact tgttgaaggg caacagctcc
     2821 gcaccatgaa agatggcggt aaaacaacga cccgggatgt actcgacttc gacctgactg
     2881 gaattgatgc ttgcgatgat cctgtcgaca tcgacttcag cagccgactc attcagcagg
     2941 acctctgatt cagcggcggc gaaatcatcg ccgccatgtg acgggctggc tgtcagtatt
     3001 tgattgtaag gattctgatc aaatcgcatg atgtccgtgt tgaattgact aggcaatgtc
     3061 ctggctaata ctcaatcttg ttcacccggt gagttgccgc caccttacac ccttggggcc
     3121 atattgcaag acggataccc cctgccagat caacgtgcag ataccggcgc tccacgcaat
     3181 aaccgcacac tccgcttgag caacgccccc agcgcataga acgccaccca gggatcgagc
     3241 aggtaatccc acagattatt cgactcgccc caaccaatcg cccatgccag caccgccagc
     3301 gcaatataca gcgcgatcaa tacttgctgc cgcatccatg ccagcagcgc aacgagcaat
     3361 aaagccgcga ggaagccgaa atgaccgaaa ccgagccgat aaggatcgaa cagcccgatg
     3421 cccagcgcga agggatagaa cgccagtgcg gcgagcgcga tcaatgccaa caagcccatc
     3481 cgcccctcgg cttcagcgcc cgacacctgc cgccgcaatg ccagcaccag caacagcagc
     3541 gaggcgacac tcaagtcacc gaagaaaccg cgcagatatc cggccaccga caatccatcg
     3601 aacggcgaca acgccagcag catgcagacg acgatatata gcaaaacggc acggcccgtt
     3661 acgcgcgcgg cgacgagcgg cacgatcagc acggccagcg caaaagccat tccagacagg
     3721 ccaacgatat cgctcaaggc catcatggct gggtctcctg ctgctcaagc caggcctgat
     3781 tgaagctcag atgcttgatg aacacgcggt tccaggtgta cagcagatgg aagtcgcccc
     3841 cgctcgcctg catcagatag ggataggaga actcgaaact gcatgcgtca cccgcgcgca
     3901 ccttggcctt ggcagagtcg acatagcgtt cgagcgacac tgcggacacc cccgctctgg
     3961 catcgctctg cttgaccaag gctgccacgc ggctcaggca tgcggcctcg tcctgcacgc
     4021 tcgtggcggt ttcattttcc agcacagcca ccctgcgcca ggacacaccg ccatccgccg
     4081 ataacatcaa cgcaagattg tcccgtccgg attgctgatc gttcaacacc gccagcagcc
     4141 gcccatccgc caacaccagc gcagcaagag ccgcatcggg attggatagc ggcgattgca
     4201 cggccgcgct ccagtgcgca ccgccatcga cagtagtcat catcttggcg cgatgcggcg
     4261 attcgttgcc ggagtagcga gtgagcacca acgcctgctg cgcgctccgc accagcacca
     4321 ccggctgcaa ggtgccctgc cctcccgccg ccagccgttg cttgtcgatc accttgcccg
     4381 attgatcgag acgcaggatt tcggcgaatt tgctgatgaa ctcgtgatac accggcagcc
     4441 ctatcgtgcc atcggcatag aggaaaggcg cggccttcac cagcgtactg atgttcatga
     4501 aaggcgagct gatcaatcga cgcggctcgc tccaggtcac accttcatcc gtcgaggtga
     4561 tcgcggtgat ggaactgccc gcccaaccgc ccagcgacac cgtgacatag aacagccaca
     4621 gcctgccgtc tgcggcacgc atcggcaccg ggttgccgac cttgccgatg taacgatgca
     4681 acccctgctg cgtgctgtcg cgactggcta tggattgctc cgccccccac tctccggtca
     4741 ccgggtcgaa caatgcgctg ttgatggtca catccgccgc accctcgcgg ctaccggaga
     4801 accagaagcc gcgcaggctt cggtctttca gctcgatgag cgaggccgca tgaacctggg
     4861 tctggaatcg tgacgaggcg aagcggctat cgaaacgcgc cgccgcaacg ttcgcgacga
     4921 cagccggacg gtactggaac gcagcgacag caggaacgtt cgccgccttg tagaacgcgg
     4981 cggcgaaggc gatcaggatg atgccgaaca gaacgcggcg atcaaacact agcggcaccc
     5041 ctccttggga ggttgtgcgg gcgcggcatt cggcgcctcg accagcaact cctgaacgaa
     5101 cagctgctga aacacttcac cgctgaagat gcgcctgatc tcttccgggc tatggcgacc
     5161 gcgtatctcc agccgctgcc cgaccaccct gcagccgacg cattgctgat gcagcggcaa
     5221 ctgcaccgcg aacagcccgt agttcgccga cagctcggcg acgctcggcg atggcaccgc
     5281 catgtagcca tgcacccgcg cacccggcaa caggaagcgg tagccctcat ccacagcgcg
     5341 gaagttgcac ggcgtccata cctctttgcc tttggcgtag cgctgcgctt ccgcgctgaa
     5401 gttgcccacc gccccatcga aggggctcag gaatgcggcc agactcaaca tcaccagcag
     5461 ggcagcccca ttcatcacca tgcgcgtgtg ccgtgccacg aacaggccga acaggatcag
     5521 cccacccgtc aacagcaaca acagccaata gaggatggaa tacaaatcgc ccacttcatg
     5581 ctgcaggcgg acagacagaa atatcagcag ggccagcacg gctcccgaca gcaacagact
     5641 cgccacgaaa aagggacggc cgatgcgctg ccagttgagc gcgcacaaca ccgccagcgc
     5701 gggcatggcc tccagcagat agcgcgccga acgctggctg ggcacgctga acacgaggaa
     5761 cagcgcaaac agccatatcc acaacattct ttcggcgttg ctcagcagcg tgcggcgccg
     5821 ccaggccacg aggaacaccg cagcgaccgg gaacgccaac aaccccgcat tgatcgggta
     5881 gccgagcagc aatgcccaga tgctgctgcc gccccacaac agcgtggcga aatatccggc
     5941 accatgagga tcgaacttgc cggcattctc gccgacgacg aattcctgcc agactgcctg
     6001 tggattggga tcgaacaaga accacaatcc gaacatcgcc agcgccaccg tcaccgtgag
     6061 cgcgaccttc caggaatcct tgagcaggaa cgccatcagg cggtagtcgc gcatgtgcag
     6121 ataccaccat gccagcgcaa aaccgacagg cacgacgagc gcgaacgatt tatacagcag
     6181 accgatgccg atctcgacac ccagcagcag cggcacgaac aggggcgact cgaaggcgaa
     6241 tgcgcggcgg tacaacaacg tacagaatgg caggaagaac cagaacacct cggcgggatt
     6301 gaccagaaat ggccgtccga agcgataggt gctgaagaag gcgaggaagg tgagtgccgc
     6361 gatgaagccc gtctccatcg gtgaggcatc gccctcgatc ttttgctgct gcgccagcct
     6421 tttcgccagc agaaacacca gcaacgccgt cagcaacgtg tagatcacac tcggataacg
     6481 caactgccac cagctccacg ccgagcccca gtgcgtcgag gcgatcccct gccagaacaa
     6541 cagcggcggc ttggtgttgc gcatgtgatc cagctcggat tgcagcggca acagatgccc
     6601 gctcgccgcc gtcagccggg tgatatgcgt gtagggaaat tcgtcaccgt ttttcggaat
     6661 attcggactg ccgagctcat agaaataagt gaacaccgcc agcaacatgg ccaggacata
     6721 ccagatcagg cgggaggtct tcatcgttca gcccgcatcc ttatccgact tgaccagcaa
     6781 cttgacgcgc ccgaaggtcc agatatcgtt cagcacaaaa ttcagcacac tggtcagccc
     6841 gatggcgatc aggttggcga tctggtagta gaagtgcggc gccagcagat tggtgaatac
     6901 cagttgcagc gcgatgctca gccagcaggc cagcgtgtat tgcccgaact gggccagcca
     6961 gggacgatga tgatgctgta cgcgatccga ccaggtccac agccggttcc agaaaaaatt
     7021 attgagcgtg gcgaagaaga tcgccagcac cagcgacagg ttcaggcgaa tctcgggctg
     7081 ctgcaccgca ttgaagatat gttcttgcgc gaaatacagc acgccgatat tgaccaccgt
     7141 gccggaggca cccaccgtac cgaatttcaa aaagcgccgc gagaatatcc ggcgcaacag
     7201 cgaaggcagc gtgatgcgca gattcagccc caatctcatg cgccggcgcc tccctgcaac
     7261 agttcgcgca ccttgtgcaa cacccggtcg ggcgtgatcc gtttcagaca ctgattgtca
     7321 ccgtcgcagg gagagttgcg atgattgtag gcggtcaggc acggcgaaca ggacagcgcc
     7381 gtgtacagac aatggatgtt cggcgccaac ggactataca gcgcaggcgt ctccggaccg
     7441 aagaacacca gcgtcggcaa tcgcgtcaat gcggcgaact gtcccggccc cccgtcgttg
     7501 gtgatcaaca gggatgcatg gttgaagatc gccagcagat gccgcaccga tttggtgtaa
     7561 ccggtgagat cgatgcaatg cggatgcgca caatgcgcca cgatctgttg cgccagcggc
     7621 ttggcatccg gcaacccgat cagggccacg gcataacctt cctgcaacag cgctgcgcac
     7681 atctgcttgt agtattcagg cggccaggcc cgtatcggca ggatgccacc gtcgggatag
     7741 accagcacca gacgcttgcc cgccagcaca ggaaagtcgg catgcaatcc gtcacccacc
     7801 tgctgcaact cgccatcgga aaattgcagc aacggcggcg cagacaactc ggcggcctcg
     7861 cccttggcga gcggcacgcc gcttgaagca atcgcatcga ccatggtcag aaactgttgc
     7921 gacaaatgcc ggtaagggtt gtacaacacc ggacggttaa tgaacgagcc gcgatacagc
     7981 ccctcctgcg tatgcggatg gaaacccaca tgcagggatg cgccggaaaa ataggcgaag
     8041 aggctgctga cccgcgagaa caattcgcaa tcgatcacca catcgcattt gagcctgcgc
     8101 aaggtgagca acgcattcca cgcatcggac acaaaaccgc ccagcgattt gtcgctgatg
     8161 gtgatcacat gctccggcgg catcacaccc agcagatcga gcacttcgcg attcttggcg
     8221 aacatcagga tatgcaagga cgcgtccgga tactgctgct tcaattttgc gaacatcggt
     8281 tgcgccagca ccagactccc catctcggac aacaggatga ccaagatgcg cttcggctgt
     8341 gccgtgcgct gagccgttcc ggccagcatg cgacgtgcgc gctcaaacac tgacagcagc
     8401 gcacagatag gcacacccgc cagacggtca acggctcgtt ggaatttgat attcaatgta
     8461 gtgcgatccg gttatcgaaa aacgccggat gcatgaggaa tagcatccgg cgcggcagga
     8521 ttatcggtta aagcgcggca ttgaacaagc cgcgctaacc gcgcggatga tgttgagcat
     8581 gcaaccgctt cagacgctcg cgcgcgacat gggtgtaaat ctgcgtggta gagatatccg
     8641 catgccccag cagcatctgc accacgcgca ggtccgcgcc gtgattgagc agatgcgtgg
     8701 cgaaggcgtg gcgcaacgta tgcggcgaca gcgacttgtg caagcccccc gccttggcgt
     8761 gcttcttgat cagataccag aacatctgcc gcgtcatacc ctccccgcgc gcggtgacga
     8821 acagcgcgtc gctgaccttg ccggacagca gcaccgcacg gccatccgtc atatagcggc
     8881 gcagccagtc cagcgcctct tcgcccagcg gcaccaggcg ctccttgctg cccttgccca
     8941 tcacgcgcac cacgcccata tccatgctca cctgcgtgat gcgtagtgtc accagctccg
     9001 acacgcgcag accagtggcg tacagcacct cgaacatggt gcggtcgcgc agacccaatg
     9061 gcgtccgcac atccggcgca gccagc
//
LOCUS       DASUIV010000066         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1318403, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000066 DASUIV010000000
VERSION     DASUIV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318403"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFW59_02440"
     CDS             <1..778
                     /locus_tag="VFW59_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5363102.1"
                     /translation="HFADYRFDMAARSVYELVWNTYCDWYVELAKVQLQDRGLSIEVA
                     EAQQRATRRTLVRVLETILRLAHPIIPFITEELWQSVAPLAGRTGESIMLQAYPKADA
                     SKIDETAIAQVAALQEMITACRSLRSEMNLSPAARVPLIAAGDAATLQALAPYLSGLA
                     KLSEVEIVAELPEGDAAIAIVGNFKLMLKVEIDVAAERVRLDKEIARLSGEIIKAEAK
                     LGNESFVARAPAAVVEQERKRMEEFGATLVQLQAQRKKLG"
     gene            complement(931..1158)
                     /locus_tag="VFW59_02445"
     CDS             complement(931..1158)
                     /locus_tag="VFW59_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX5363103.1"
                     /translation="MNFDVELDVRQLACPLPILRAKKSLAGMSSGQVLKIVATDKGSP
                     NDFIDFCKKTGNELRASTEADDEFTFLIRRR"
     gene            complement(1158..1472)
                     /locus_tag="VFW59_02450"
     CDS             complement(1158..1472)
                     /locus_tag="VFW59_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5363104.1"
                     /translation="MADFKNITVDELQVMLAQGALRLVDVRTDTEVAHGKIPQGESMP
                     LHLIPMRINEMDKAATTVFYCQMGGRSAQAAAFAAGNGFANVYNLQGGITAWARAGLP
                     IA"
     gene            1594..3030
                     /locus_tag="VFW59_02455"
     CDS             1594..3030
                     /locus_tag="VFW59_02455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5363105.1"
                     /translation="MKKLTLLLLLTLSHQTLGEGLPDLGDTSQLVLTPQQERQIGQQS
                     MLQIRAGKQYFNDSEINDYLNQLGYRLVENSAEPGLGFEFFMLDDYNINAFALPGGFI
                     GVNSGLLLTSQSESELASVLSHEIAHVTQHHLARMIAGTKNDSLASMAAVAIAILAAR
                     SNSQASQAAIAGAQAHAIQNRLDYTRTHEQEADRIGFETLLKSGFNTHAMPEFLNRMQ
                     TATRLLEGNMPGYLRTHPITSERIADIENRAQKQPYRLIPDSLDFQLVRTKLIARQKT
                     AKDAIGYFSDALGPQKHGNPVAQRYGLVISLLRNNEIERAAQELLVLRKQTRSDIAAR
                     RNAMIETLTGRVKRAAPDNTDALSFYRTAVQDFPQHRALIYDYAELLIDANQADQAAK
                     LLAEQIIRHPSDTTLYELLARSHAKQGNRFEQHQAQAYGYAWQGKLFPAIEQLELAKQ
                     AGGSFYQLSVIESDLRELREMAEANAKR"
     gene            complement(3041..>3344)
                     /locus_tag="VFW59_02460"
     CDS             complement(3041..>3344)
                     /locus_tag="VFW59_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5363106.1"
                     /translation="EAGQYELALTDYRHVIELMPDSPAAYTNMGSIYLRAQQWQKAVE
                     SFDKAIEIMKSAGKEFDSGKPYFVRAIASEQLGNHQQAREDYRVSCRIEFLGCDKL"
BASE COUNT          765 a         1061 c          905 g          613 t
ORIGIN      
        1 acacttcgcc gactaccgtt tcgacatggc tgcacgttcg gtctatgagc tggtctggaa
       61 cacctactgc gactggtatg tggaactggc caaggtgcaa ctacaggatc gaggattgag
      121 tattgaggtt gccgaggcac agcagcgcgc cacgcgacgc accttggtgc gcgtgctgga
      181 gaccatcctg cgccttgctc acccgatcat ccccttcatc accgaagagc tgtggcaatc
      241 ggtcgcaccg ctggcaggca ggacaggcga gagcatcatg ctgcaggcct atcccaaggc
      301 ggatgcaagc aagatcgacg agaccgccat cgctcaggtc gcagcattgc aagagatgat
      361 caccgcctgc cgcagcctgc gcagcgagat gaacctgtcg ccggctgctc gtgtgccgct
      421 gatcgcggcg ggtgatgccg ccacgcttca ggcactcgcg ccctacctca gcggtttggc
      481 caaactcagc gaagtcgaga tcgtcgccga actgccggaa ggcgatgcgg cgatcgccat
      541 agtcggcaac ttcaaactga tgctgaaggt agagatcgac gtcgcggcag agcgcgtgcg
      601 tctggacaag gagattgcgc gcctgtccgg cgaaatcatc aaggcagaag ccaagctcgg
      661 caacgaaagc ttcgtcgccc gcgcacccgc agccgtagta gaacaggaac gcaaacgcat
      721 ggaagagttt ggcgcaacgc tggtgcagtt acaggcacag cgcaaaaagc tgggttaatg
      781 caaaaagcag tcatccgcag attgcgcaga tttccgaccg ttatatgtaa aggaaggctc
      841 accgagcagg tgacctcaca ttggttttgt ttaatcggcg aaaatctgtg taatctgcgg
      901 acgagacttc ggtttttcgg ttgaacgccc ctagcggcgg cggatcagga acgtgaattc
      961 atcgtccgct tcggtcgagg cccgcaactc gttgcctgtt ttcttgcaga aatcgatgaa
     1021 atcattcggc gagcccttgt cggtcgcgac gattttcagc acctgtccgc tgctcatgcc
     1081 tgccagcgat ttcttggcgc gcaggatagg caacggacag gccagttggc gcacatccag
     1141 ttcaacgtcg aagttcatca cgcgatcggc agacctgccc gcgcccaggc ggtgatgccg
     1201 ccctgcaggt tgtacacatt cgcaaagccg ttccctgcgg caaaggcggc agcctgcgcc
     1261 gagcgcccgc ccatctggca ataaaagacc gtggtcgcgg ctttgtccat ctcgttgatc
     1321 cgcatcggaa tgaggtgcag cggcatggac tcgccctgcg ggatcttgcc gtgagccacc
     1381 tcagtatcgg tgcgcacatc caccagacgc aaagcaccct gcgccagcat cacctgcaat
     1441 tcgtcgaccg taatgttttt aaaatctgcc atatttccgt tccttgtttc cgaagggcgg
     1501 cgattctaac gcaaatcggc tccgcaacct atgcctcacc catgctgccc gacatgaaag
     1561 gaacgttcag caaaagcggt tatcatatcg accatgaaga aattaacctt gctgctgttg
     1621 ctgaccctct cacaccaaac cttgggcgag gggctgcccg acctggggga cacctcgcaa
     1681 ctcgtgctca caccgcagca agagcgccag atcggccagc agagcatgtt gcaaatccgc
     1741 gccggcaaac agtatttcaa cgattcagaa atcaacgact acctcaacca gctcggctac
     1801 aggctggtcg agaacagcgc cgagcccgga ctgggcttcg agttcttcat gctcgacgat
     1861 tacaacatca acgccttcgc cctgcccggc ggtttcatcg gggtaaacag cggcctgctg
     1921 ctcacctcgc agagcgaatc ggaattggcc tccgtgctga gccacgagat cgctcacgtc
     1981 acccagcacc atctggcgcg catgatcgcg ggcacgaaaa acgacagcct ggcctcgatg
     2041 gcagcagtcg cgatcgccat cctcgccgcg cgcagcaact cgcaagcatc gcaggctgcc
     2101 atcgccggcg ctcaggctca cgccatccag aaccgactgg actacacccg cacgcacgaa
     2161 caggaagcgg accgcatcgg cttcgagacc ctgctcaagt ccggtttcaa cacccatgcc
     2221 atgccggaat ttctgaaccg gatgcagacc gccacccggt tgctggaagg caatatgccg
     2281 ggttatctgc gcacccaccc gatcaccagc gaacgcatcg ccgacatcga gaatcgcgca
     2341 cagaaacagc cctaccgttt gataccagac agcctcgact tccagctggt gcgcaccaaa
     2401 ctcatcgccc gccagaagac cgcgaaggat gccatcggct acttcagcga tgcgctcggc
     2461 ccccagaaac acggcaaccc ggtcgctcag cgctatggac tggtgatatc gctactgcgc
     2521 aacaacgaga tcgagcgagc cgcgcaggaa ttgctagtgt tacgcaaaca gacccgaagc
     2581 gacattgcgg cgcggcgcaa cgccatgatc gagacgctca ccggacgggt aaaacgcgcc
     2641 gcaccagaca ataccgacgc gctgagcttc taccgcacgg ccgtgcagga tttcccgcaa
     2701 caccgcgccc tgatctacga ctatgccgaa ctgctgatcg atgccaatca ggccgaccaa
     2761 gccgcaaaat tgctagccga acaaatcatc cgccatccca gcgacaccac gctgtatgaa
     2821 ctgctggcgc gcagccatgc caaacagggc aatcgcttcg aacaacatca ggcgcaggcc
     2881 tacggctacg cctggcaagg caagctgttc cccgctatcg aacaactgga actagccaaa
     2941 caggccggcg gcagcttcta ccagctctcc gtgatcgaga gcgacttgcg cgaactgcgc
     3001 gagatggcgg aagcgaacgc caaacgttaa aatgccccgg tcacagctta tcgcatccca
     3061 gaaactcaat ccggcaactg acccgatagt cctctctggc ctgttggtgg ttgcctagct
     3121 gttcagacgc gatggcccgc acaaaatagg gcttgccgct atcgaattct ttgccggccg
     3181 acttcatgat ttcgatagcc ttatcgaaac tttcgacagc cttctgccat tgttgcgccc
     3241 gaagatatat cgagcccata ttggtatacg ctgcggggga atctggcatc aattcgatca
     3301 catgcctgta atcggtcagc gccagctcat attgaccggc ctcc
//
LOCUS       DASUIV010000067        17232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1225777, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000067 DASUIV010000000
VERSION     DASUIV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225777"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFW59_02465"
     CDS             complement(<1..849)
                     /locus_tag="VFW59_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5363107.1"
                     /translation="MSNIQSVLNETRVFPPSDEFVKQANVSGMASYQALCAEAERDYQ
                     GFWGRLARENIAWKQPFEKILNDSNAPFYKWFEDGQLNVSHNCLDRHLATQPEKTAII
                     FEADDGTVSKVSYRELHGRVSQFANGLKSRGIKKGDRVVIYLPMSIEAIVAMQACARI
                     GAIHSVVFGGFSSKSLHERIVDAQACAVITADAQMRGGKVMPLKTIVDEALAQEGCDC
                     VRNVIVYRRAGSSVDWNDERDIWWHDLFNAQPTHCEPEWVDAEHPLFILYTSGSTGKP
                     KGVQHSS"
     gene            1052..2707
                     /locus_tag="VFW59_02470"
     CDS             1052..2707
                     /locus_tag="VFW59_02470"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5363108.1"
                     /translation="MTKYIFITGGVVSSLGKGIAAASLAAILESRGLKVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFISAKMRKANNFTTGQIYDSVIRKE
                     RRGEYLGKTVQVIPHITNEIQAFVERGAAASHDGKADVAIVEIGGTVGDIESLPFLEA
                     ARQMGLRMSRNQVAYVHLTLVPYIATAGELKTKPTQHSVQKLREIGIFPTALVCRADR
                     PIPDDERSKISLFANVREEGVISMWDVDSIYKVPQMLHEQGLDRIVCEELALNAPPAD
                     LSVWAKLVTALENPQHQITIGMVGKYVDLTESYKSLIEALRHAGIHTSTKVNIEYLDS
                     EDIETSGADVLARYDAILVPGGFGKRGTEGKIAAIRYARENKVPYLGICLGMQLAVIE
                     YARHVAGMTDANSTEFNLESEHPVVALITEWLDRDGKVATRSADSDLGGTMRLGSQRC
                     PIKPGTLAQRIYGDEVNERHRHRYEVNNHYVPALEQAGLIIAARTPSEDLPEIMELPQ
                     SLHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALLSQSKKVAV"
     gene            2704..3555
                     /gene="kdsA"
                     /locus_tag="VFW59_02475"
     CDS             2704..3555
                     /gene="kdsA"
                     /locus_tag="VFW59_02475"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX5363109.1"
                     /translation="MKLCHFEAGLDQPLFLIAGPCVIESEQMAIDTAGALKEICSELN
                     LNFIYKSSYDKANRSSGKSFRGFGLDAGLKILGEVKSQLGLSVLTDVHSIEEIAPVAA
                     VVDVLQTPAFLCRQTDFIHAVAKCGRPVNIKKGQFLSPWDMQNVVEKAREASGADNIM
                     VCERGASFGYNNLVSDMRSLAVMRNTGCPVVFDATHSVQLPGGQGNVSGGQREFVPVL
                     ARAAVAAGVSGLFMETHPNPSQALSDGPNAFPLAHLKALLKTLMVLDATVKQQGLIEE
                     KIDLRNV"
     gene            3580..4863
                     /gene="eno"
                     /locus_tag="VFW59_02480"
     CDS             3580..4863
                     /gene="eno"
                     /locus_tag="VFW59_02480"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5363110.1"
                     /translation="MSAIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     TKEAVELRDGDKKRYLGKGVLKAVEYVNTEIAEAIIGLDASEQAFIDQTMIDLDGTEN
                     KSRLGANAILAVSIAVAKAAAEESGLPLYRYFGGAARMEMPVPMMNVINGGAHATGGG
                     DMQEFMIVPVGAQSFREALRCGAEVFHALKDVLHHKGFATTVGDEGGFAPALGSNEGA
                     LKVIVEAIEAAGYVPGADVAIAMDPASSEFYKDGQYHLKADGLVLNSAQMVDMYATWV
                     DKYPIISIEDGMSEHDWDGWKLLTERLGKNVQLVGDDLFVTNTKILREGIKKGVANSI
                     LIKVNQIGTLTETFAAIEMAKRAGYTAVMSHRSGETEDSTIADLAVGTNVMQIKTGSL
                     SRSDRMAKYNQLLRIEEDLGDCASYPGREAYYQLG"
     gene            4863..5171
                     /gene="ftsB"
                     /locus_tag="VFW59_02485"
     CDS             4863..5171
                     /gene="ftsB"
                     /locus_tag="VFW59_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEX5363111.1"
                     /translation="MRAVTLILAVLLLLLQYPLWLGKGSWLKVWDLNRQVTEQQQVNQ
                     QTQTRNAMLDAEVLDLKQGTEAIEERARSELGMIKRDEVFYQFIGKQPAAVSAVAAGR
                     "
     gene            5194..7026
                     /gene="recQ"
                     /locus_tag="VFW59_02490"
     CDS             5194..7026
                     /gene="recQ"
                     /locus_tag="VFW59_02490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX5363112.1"
                     /translation="MATPQQILHDVFGYASFRGAQQAIVEHVVAGGDALVLMPTGGGK
                     SLCYQIPSLLRPGIGIVVSPLIALMQDQVDALKQLGVSAAFLNSSLDADAAREVYRQI
                     NRGELKLLYVAPERLMTEGFLSLLERLDEENGIALFAIDEAHCVSQWGHDFRPEYRAL
                     TVLHERFPSVPRIALTATADAPTRREIVERLSLEQAEQFVSSFDRPNIRYRVALKNNA
                     RQQLQAFLENEHPNDAGIVYCLSRKKVEETAAWLKEQGWDALPYHAGLDAATRNKNQR
                     RFLREEGVIMVATVAFGMGIDKPNVRFVAHLDLPKSMEGYYQETGRAGRDGQPANAWM
                     AYGLGDVVSMRQMLLSGDAPEERKRVELQKLDALLGFCESTDCRHQTILRYFGEEHPG
                     GCAQCDNCLEPVDTWDATQAAQMALSCVYRTGQRFGVAHLIDVLLGKETPKVVQFNHR
                     ELSTFGIGKELTQQQWSSVYRQLVAAGYINVDMEAYGGLKLSEAARPVLKGLQEVWLR
                     RDADPVKRKSLRAEGKAKSREPFAGASDDVLWLALKAKRTELAREQGVPPYVIFHDST
                     LLEIHNHRPHTLDEMGKISGVGQAKLAKYGDAFLQVVEDVANGV"
     gene            7136..7840
                     /locus_tag="VFW59_02495"
     CDS             7136..7840
                     /locus_tag="VFW59_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5363113.1"
                     /translation="MGAQWKARHKEVAANAKGKIFGKLAKEIMVAAKSGADPDLNSRL
                     RLVVEQAKKASMPKETLDRAIKKGAGLLDGGAQLERLVYEGFAPHRVPVIVECLSDNI
                     NRTMSSMRVLFRKGQLGAEGSVSWDFSYVGMIEATPNSKESDPELAAIEAGAQDFEPS
                     EEGATLFITDVTDLDAVSKALPEQGFTVISAQLGYRPKNPVTLASDAEREEVEAFLEA
                     MDGDDDVQNVYVGLAG"
     gene            7840..8496
                     /locus_tag="VFW59_02500"
     CDS             7840..8496
                     /locus_tag="VFW59_02500"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEX5363114.1"
                     /translation="MEKEFWLTRWERAEIGFHQDEVNPYLCQYWPELDRHPGCEVFVP
                     LCGKSLDMLWLRQQGATVLGVELSSIAVQGFFAENGYAPQHLCSEKFDCCEADGIRIL
                     CGDFFDLNREDLAQVTTVYDRASLVALPPDMRERYARHLVSILPAGTQILLVTFDYPQ
                     SEMSGPPFAVTPDEVDALYRDHAEIRLLAQWDVMAQNPRFEKRGVTRLQENIFLLTLN
                     "
     gene            8579..8959
                     /gene="gloA"
                     /locus_tag="VFW59_02505"
     CDS             8579..8959
                     /gene="gloA"
                     /locus_tag="VFW59_02505"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX5363115.1"
                     /translation="MRILHTMIRVGDLDRSIDFYTQVLGMKLLRRKDYPEGKFTLAFV
                     GYGEESSQAVIELTHNWDTGSYELGTGFGHVAIEVGDVYAACEEIKRRGGAVVREAGP
                     MKGGTTVIAFVRDPDGYMIELIGA"
     gene            complement(8986..9741)
                     /locus_tag="VFW59_02510"
     CDS             complement(8986..9741)
                     /locus_tag="VFW59_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEX5363116.1"
                     /translation="MTTPVRLAKRLAALVPCSRREAELYIAGGWVMVDGQVVEEPQFM
                     VAEQRVELHPDANLTPVEPVTILLHQPPDGRAPLPNPLPPAGEGANESLRELRITADT
                     HWADDPSGIHLLKQHFLHLTQCTPLERNAGGLLVFTQDWRVTRKLTDDAATVEQEYVV
                     EVAGELSPDGLKLLNHGLTFNGKPLPPIKVSWQNETRLRFALKGVQPGAIAHMCDKVG
                     LQVLSMKRIRIGGVPMSKLPPGQWRYLMPQERF"
     gene            9815..10039
                     /locus_tag="VFW59_02515"
     CDS             9815..10039
                     /locus_tag="VFW59_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HEX5363117.1"
                     /translation="MDANNKPVSLPDGITLEAALVYLFERMGWESMAAAVPIRCFSHD
                     PSIKSSLKFLRRTPWARAKVEQLYLQKIDR"
     gene            complement(10307..11161)
                     /gene="folD"
                     /locus_tag="VFW59_02520"
     CDS             complement(10307..11161)
                     /gene="folD"
                     /locus_tag="VFW59_02520"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX5363118.1"
                     /translation="MTARIIDGKAIAQEVRAEWKVRADALKARGITPGLAVIIVGEDP
                     ASKVYVANKVKACAELGLHSEHIALSADTSEADLLAKIAELNADPKIHGLLVQLPVPK
                     HIDGDKVLNAISPDKDVDGFHPMNVGALATGNMRFAPCTPYGSLILLQKSGVTIEGKH
                     AVVVGRSNIVGKPMALLLLQHNATVTICTSKTVDLAKFTRDADILVVATGKPKMITGD
                     MIKPGAAVIDVGINRMADGKLCGDVDFDSAKEVAGWITPVPGGVGPMTITMLVANTVQ
                     AAERVAGK"
     gene            complement(11181..11720)
                     /gene="ppa"
                     /locus_tag="VFW59_02525"
     CDS             complement(11181..11720)
                     /gene="ppa"
                     /locus_tag="VFW59_02525"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5363119.1"
                     /translation="MSLLNVRPGKKLPHECNVIIEIPKHADPVKYEVDKDAGVLVVDR
                     FIGSLMRYPANYGFIPETIADDGDPVDVLVVTPFPIVHGAIIACRPVGVLMMSDEGGD
                     DAKIIAVPTDKLTPLYRDVKTYSDLPEITLEQIKHFFELYKALEPGKWVKIRGWEGVE
                     AAHKEITNGLAAYQASLNK"
     gene            11839..13032
                     /locus_tag="VFW59_02530"
     CDS             11839..13032
                     /locus_tag="VFW59_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5363120.1"
                     /translation="MATQNKLIGKMVIGQSGGPTAVINQSLVGAVLAARGQANITGIL
                     GARHGIAGIMQEDFIDLTTQSVEQLELVATTPAAALGSVRLKPGRAECEKVFEVFKKN
                     DVRYFFYIGGNDSAETAHIIAEMAKEANYDFCTVHIPKTIDNDLKVTDHCPGYASAAR
                     FVALAFMGDDRDNRALAGVKVNVVMGRSAGFLTAASALARQAEGDGPHLIYLPERVFD
                     VAQFKQDVRDAMAKYGRCVVAASEGISDKDGNPISTSGEKDSHGNVQLSGSGALGDTL
                     AALVKEAYAGQKVRVRADTFGYLQRSFPTIVSEIDAREARAVGDYAVKHATGTGLPGS
                     VAIRRLSSKPYASECFITPLSSVAREATKMKDEYINAAGNDVTQAWLDYVAPLVGELP
                     KMGRL"
     gene            complement(13172..13519)
                     /locus_tag="VFW59_02535"
     CDS             complement(13172..13519)
                     /locus_tag="VFW59_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX5363121.1"
                     /translation="MITIEQTEHLVNAAVIGEFTLADFKKFEEESLHKLKSTGPINLL
                     FDLRGMVDYTIDVAWEEIKFFNREHNHDFNKIAVVTDDQWITWHAWLSRLFVDANTRA
                     FADYDEALAWTQE"
     gene            complement(13522..14382)
                     /locus_tag="VFW59_02540"
     CDS             complement(13522..14382)
                     /locus_tag="VFW59_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5363122.1"
                     /translation="MHKQAGSLWMLLAGFLFGCMGVFVKLGATHFSHIELVFYRSFFG
                     LLLVYAILRQQRVSLATKHWQPHLWRGLSGTVALALFFYCITMLPLATAVTLNYTAPL
                     FLTIFTMLVFKDRFNLPLTCTIALGFCGVVLLLHPTLERDQLWLGLLGLISGFLAGIA
                     YLNVKQLGMQGEPATRIVFYFSLIASIGSGAWMLFDEVHAVTAQGLLILLGLGGTATL
                     AQLAMTRAYQVGKTLVVGSMAYSTILFASLFGMLLWNETLTASSWLGMALIVASGVLS
                     LRLSPKHQET"
     gene            complement(14375..14875)
                     /locus_tag="VFW59_02545"
     CDS             complement(14375..14875)
                     /locus_tag="VFW59_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5363123.1"
                     /translation="MHLHPLRHMTPTFLIENISSRPDLLDSICDWHRREWGEEWAEQV
                     RQSTNKDRIPTVYVAIENGQPIGTAMLVNDDMTTHPEISPWLGGVYVKPEHRGRGIAT
                     ALSRHAMHEAARLGVSRLWLYTVSARPLYESLGWEYQREEDYLGERATIMKCDLNRLA
                     DIARRA"
     gene            complement(14848..15708)
                     /locus_tag="VFW59_02550"
     CDS             complement(14848..15708)
                     /locus_tag="VFW59_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363124.1"
                     /translation="MNDLNLYAERLKIHGNFNMTTGAMHKNINTTYWRIGRFLLRHIW
                     MLGVVLALGILFFLYKLLLLGTLFLSASPASVNNDWNAIGSAGDWTSALATIAGFLFL
                     GYQLRREQQVLEVQTSAQVYGSGIDVLKLFIENHELRPYFYDCKPSPEKFPDDGAWDR
                     VMTACEIMCDQFESFYSSQNSLNNEVSAIWVRYMCGIYLTSPSLRYFLVQEGYRYDDD
                     FIGVFNCNLHGISTRTRERIENLARETRADTESSNRFVLRRHDLTLEALAELQSRQTN
                     EQCTCTRCAT"
     gene            15902..16840
                     /locus_tag="VFW59_02555"
     CDS             15902..16840
                     /locus_tag="VFW59_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEX5363125.1"
                     /translation="MSLFALIAALLLEQLHPLSARKYLYGWLTGYANFYQLHFNAGEL
                     RHGKFAWLFAVLPLLFGTILLYWLLYRVHPFLAWAFNVLVLYLTMGFRQFSHYFTDIH
                     KALRDGELERARGLLTSWRGEPAHELNAEEVARVTIEEAMLASHRNVFGVIVWFVLFS
                     VLGLGGAAGALLYRMGQFLRARWGDEEDDDLELFGNFSRKAFHVLEWLPIRLTAMTFA
                     IVGDFEDTVYCWRTQAASWPDGDDGILLASGAGALGVRLGMPIPQGGLPFDRPELGVG
                     EDADADFMQSTIGLVWRSVVFWLLLLLLLTLASLLG"
     gene            16908..>17232
                     /locus_tag="VFW59_02560"
     CDS             16908..>17232
                     /locus_tag="VFW59_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5363126.1"
                     /translation="MSRESIVGKLKSALDNLERLPAMPAIAHKLLALPLDTDAGEAQL
                     IVLIEQDPQLSAKILGLANSPVMGVGRKVAGIKDAAMLLGFKRLKSVAIGIATMSRFT
                     NQPAVK"
BASE COUNT         3398 a         4866 c         5452 g         3516 t
ORIGIN      
        1 gctggaatgt tgcacgccct tgggcttgcc cgtggagccg gaggtataga ggatgaacag
       61 cggatgttcc gcatccaccc attccggttc gcaatgcgtg ggctgtgcat tgaacaggtc
      121 gtgccaccag atgtcgcgtt catcgttcca gtcgacgctg gagccggcac ggcgataaac
      181 gatgacgttc cggacacagt cgcacccttc ctgtgccagc gcctcgtcca cgatggtttt
      241 cagcggcatc accttgccgc cgcgcatctg cgcatcggcg gtgatgacgg cgcaagcctg
      301 ggcgtcgacg atgcgctcat gcaggctctt ggaggagaag ccgccgaata ccaccgaatg
      361 gatcgcgccg atgcgggcgc aggcctgcat cgccacgatg gcctcgatgc tcatcggcag
      421 gtagatcacg acgcggtcgc ccttcttgat gccgcgcgac ttcaggccgt tggcaaactg
      481 gctgacgcgt ccgtgcagtt cgcgatagga gactttgctg actgtgccat cgtccgcctc
      541 gaagatgatc gcggtttttt ccggctgggt ggcgagatgg cgatccagac agttgtggga
      601 gacattcaat tggccatctt cgaaccactt gtagaacggg gcattgctgt cgttcagtat
      661 cttttcgaaa ggttgtttcc aggcgatgtt ttctcgtgcc agtcgccccc agaatccctg
      721 atagtcgcgc tcggcttcgg cgcacagcgc ttgatagctt gccatgcccg agacgttggc
      781 ttgtttgacg aactcgtccg aaggcgggaa aacgcgggtt tcattcagaa cagattggat
      841 attggacatg gcgcgctcct tggctggcga ttgtgattgg gctgacgatt gcgatcaggc
      901 tggttgggct gacccgactc agggccagca actttagttt tttccctgcg cactgtcaac
      961 gaatacgccg cccgaagtca atgggaatat cctgattaag tagggcatta cgattttgat
     1021 cgggaggggt tttgcggtat catcgcgctc catgaccaaa tacatattta ttaccggcgg
     1081 cgttgtctct tccttaggga aggggatcgc cgccgcttca cttgcggcca tcctcgagtc
     1141 gcgcgggctc aaggtgacga tgcttaagct cgatccctat atcaacgtcg atccgggcac
     1201 gatgagtccg ttccagcacg gcgaggtgtt cgtcaccgaa gacggcgcgg agaccgatct
     1261 ggatctgggg cattacgagc gtttcatctc cgccaagatg cgcaaggcca acaacttcac
     1321 cacaggccag atatacgaca gcgtgatccg caaggagcgg cgcggcgagt atctgggcaa
     1381 gacggtgcag gtgattccgc acattaccaa cgagattcag gccttcgtcg agcgcggcgc
     1441 tgccgcatcg catgatggca aggcagacgt ggcgatcgtc gaaatcggcg gtacggtcgg
     1501 cgacatcgaa tcgctgccct tcctcgaggc cgcgcgccag atggggttgc gcatgagccg
     1561 caaccaggtt gcctatgtgc acctgacgct ggtgccctac atcgctaccg ccggcgaact
     1621 gaagaccaag ccgacccagc acagcgtaca gaagctgcgc gagatcggca tcttcccgac
     1681 cgcgctggtg tgccgtgccg atcgccctat tcccgatgat gagcgctcca agatttccct
     1741 gttcgccaat gtgcgcgaag agggggtgat ctcgatgtgg gacgtggaca gcatctacaa
     1801 ggtgccgcag atgttgcacg agcaggggct ggatcgcatt gtctgcgaag agctggcgtt
     1861 gaatgcgccg cctgccgacc tgtcggtgtg ggcgaagctg gtgaccgcgc tggagaaccc
     1921 gcagcatcag atcaccatcg gcatggtcgg caaatatgtc gacctgaccg agtcctacaa
     1981 gtcgttgatc gaggcgttgc gccatgcggg catccatacc tcgaccaagg tcaatatcga
     2041 atatctggac tcggaagaca tcgagacttc gggggcggac gttctggctc gctacgatgc
     2101 gatcctggtg ccgggcggat ttggcaagcg cggtaccgaa ggcaagatcg ccgcgatccg
     2161 ctatgcgcgt gagaacaagg tgccctattt gggcatctgt ctcggcatgc aattggctgt
     2221 gatcgagtat gcgcgccacg tcgcaggcat gaccgacgcg aacagcaccg aattcaatct
     2281 ggaatccgag catccggtcg tcgcactgat caccgagtgg ctggatcgcg atggcaaggt
     2341 ggcgacacgc agcgccgatt ccgatctggg cggcaccatg cgtctcggtt cgcagcgttg
     2401 cccgatcaag ccaggcacgc tggcgcaacg catctacggc gacgaggtga acgaacgtca
     2461 ccgtcatcgt tacgaggtga acaaccatta cgttcccgcg ctggaacagg ccgggctgat
     2521 catcgctgca cgcacgccgt ccgaggatct gcctgagatc atggagttgc cgcagtcctt
     2581 gcacccgtgg ttcgtcggcg tacagttcca tcccgagttc acctcgacgc cgcgcgatgg
     2641 tcatccgctg ttcaaggcct atgtcgaggc cgcattgttg tctcagtcca agaaggtggc
     2701 ggtatgaagc tgtgccattt cgaggcgggg ctggatcagc ccttgttcct gatcgcgggg
     2761 ccctgcgtga tcgaatcgga acagatggcg atcgataccg ccggagcgct gaaagaaatc
     2821 tgtagcgagt tgaacctgaa tttcatctac aagtcctctt acgacaaagc caaccgcagt
     2881 tccggcaagt cgttccgagg gttcggactg gatgcggggt tgaagatact cggcgaagtg
     2941 aagtcgcagc tggggttgtc ggtattgacc gatgtgcaca gcatcgagga gatcgcgccg
     3001 gtcgctgcgg tggtggatgt gttgcagacg cccgccttcc tgtgccgtca gacggacttc
     3061 atccatgcgg tcgcgaaatg cggtcgcccg gtgaacatca agaaggggca gttcctgtcg
     3121 ccctgggaca tgcagaacgt cgtggagaag gcgcgcgagg ccagcggcgc cgacaatatc
     3181 atggtgtgcg agcggggcgc ctcgttcggc tacaacaatc tggtgtcgga tatgcgttcg
     3241 ctggcggtga tgcgcaacac cggttgtccg gtggtgttcg atgcgacgca ttcggtgcag
     3301 ttgccgggcg ggcagggcaa tgtttccggc ggtcagcgcg agttcgtccc cgtgctggct
     3361 cgggcggcgg tggcggcggg tgtgtccggc ctgttcatgg agacccatcc gaacccgtcc
     3421 caggcgctgt cggacggccc gaatgccttt ccgctggccc atctcaaggc gctgttgaag
     3481 acgctgatgg tgctggatgc aacggtcaag cagcagggat tgatcgaaga aaaaattgat
     3541 ttaagaaacg tttagataac gcatacaagg aagaaaaaga tgagtgcaat tgttgatgtg
     3601 atcgcccgtg aaattctcga ttcccgtggt aacccgaccg tagaagcgga cgtgttgctc
     3661 gagtccggcg tgatggggcg tgccgccgtg ccgtccggcg cttccaccgg taccaaagag
     3721 gccgtggaat tgcgcgatgg cgacaagaaa cgctatctgg gcaagggtgt attgaaggcg
     3781 gtggaatatg tgaacaccga gatcgccgaa gcgatcatcg gtctggacgc ttccgagcag
     3841 gccttcatcg accagaccat gattgatctg gacggcaccg agaacaaatc ccgtctgggc
     3901 gcgaacgcga ttctggcggt gtccatcgca gtcgccaagg cggctgccga ggagtcgggc
     3961 ctgccgttgt accgctactt tggcggtgcg gcgcgcatgg agatgcccgt gccgatgatg
     4021 aacgtcatca acggcggcgc gcatgcgacc ggtggcggcg acatgcaaga gttcatgatc
     4081 gtgcccgttg gcgcacagag cttccgtgaa gccttgcgtt gcggcgccga ggtgtttcac
     4141 gccctgaagg atgtgttgca tcacaagggt ttcgcgacga cggtcggcga cgaaggcggt
     4201 ttcgcgccag cgctgggttc taacgaaggc gctttgaagg tcatcgtgga agcgatcgaa
     4261 gcggccggtt atgtgccggg tgcggacgtg gcgatcgcca tggatcccgc ttcctcggaa
     4321 ttctacaagg acggtcaata ccatctgaag gctgacggtc tggtgctgaa ctcggcgcaa
     4381 atggtcgata tgtacgcgac ctgggtggac aagtatccga tcatctctat cgaagacggc
     4441 atgagcgaac atgactggga cggctggaaa ctgctgaccg agcgcctggg caagaacgtg
     4501 caactggtcg gtgacgactt gttcgtgacc aacaccaaga tcctgcgcga aggcatcaag
     4561 aagggtgtcg ccaattccat cctgatcaag gtcaaccaga tcggtacctt gaccgagact
     4621 ttcgccgcca tcgagatggc caagcgcgcc ggttacaccg cagtgatgtc gcaccgctcc
     4681 ggcgagaccg aagactccac catcgccgat ctggcggtgg gcaccaacgt gatgcagatc
     4741 aagaccggtt cgctgtcgcg ctccgatcgc atggcgaaat acaaccagct gctgcgtatc
     4801 gaagaagacc tgggcgattg cgcatcctac ccagggcgtg aggcctacta ccagcttggc
     4861 tgatgcgtgc cgtcacactg atattggccg tcctcttgct cctgttacag tacccgctgt
     4921 ggctgggcaa gggtagctgg ttgaaggtgt gggacctgaa ccgtcaagtc accgaacagc
     4981 agcaagtcaa tcagcagacc cagacacgca acgcgatgct ggatgccgag gtgctcgatc
     5041 tcaagcaggg caccgaagcc atcgaggaac gcgcccgcag cgagctgggc atgatcaaac
     5101 gcgacgaagt gttctatcag ttcatcggca aacagcctgc ggcggtctcc gccgtggccg
     5161 ctggccgcta gccgtaccat tccgacggca tccttggcca ctccacaaca aatcctccat
     5221 gacgttttcg gctatgcatc ctttcgcggt gcgcagcagg ctatcgtcga gcatgtcgta
     5281 gcgggcggcg atgcgctggt gctgatgccc accggcggcg gcaagtcgtt gtgttaccag
     5341 attccttcct tgttgcgtcc gggcatcggt atcgtggtgt cgccgctgat cgcactgatg
     5401 caggatcagg tcgatgcgct caaacagctc ggtgtgtcgg ccgccttcct gaactccagt
     5461 ctggatgccg atgcggcacg cgaggtgtac cggcagatca atcgcggcga gctgaagctg
     5521 ctgtatgtcg cgcccgagcg gctgatgacc gaaggctttc tgagtctgct ggagcgtctg
     5581 gatgaggaga acggcatagc gctgttcgct atcgacgagg cgcactgcgt gtcgcaatgg
     5641 gggcacgatt tccgtcccga atatcgtgcg ctgacggtgt tgcacgagcg ttttccttct
     5701 gtgccgcgca tcgcgctcac cgccactgcc gatgccccga cgcggcgcga gatcgtcgag
     5761 cgtctgtcgc ttgagcaggc ggagcaattc gtctccagct tcgaccgtcc caatatccgt
     5821 taccgcgtcg cgctgaagaa caatgcgcgc cagcagttgc aggcctttct tgaaaacgag
     5881 catcccaacg acgcgggcat cgtgtattgc ctgtcgcgca agaaggtcga ggagaccgct
     5941 gcctggctca aagagcaggg ttgggatgcg ctgccttacc atgccggact ggatgcggcg
     6001 acgcgcaaca agaatcagcg cagattcctg cgcgaagaag gcgtcatcat ggttgccacc
     6061 gtcgcgttcg gcatgggcat cgacaagccc aacgtgcgct tcgtcgccca cctcgatctg
     6121 cccaagagca tggaaggcta ttatcaggaa acggggcgtg ccgggcgcga tggacagccc
     6181 gcgaatgcgt ggatggccta tggtctgggc gatgtggtgt ccatgcgtca gatgttgttg
     6241 tcgggcgatg cgcccgaaga gcgcaagcgt gtcgagctgc agaagctcga tgccttgctg
     6301 ggcttttgcg agtccaccga ctgccgccat cagaccatcc tgcgctattt cggcgaagaa
     6361 catccgggcg gttgcgctca gtgcgacaat tgcctcgagc cggtcgacac ctgggatgcg
     6421 acgcaggccg cgcagatggc gctgtcctgc gtctatcgca ccggccagcg cttcggcgtc
     6481 gcgcatctga tcgatgtgct gctgggcaag gaaacgccca aggttgtgca gttcaaccat
     6541 cgcgaactga gcaccttcgg tatcggcaag gagctgacgc agcagcaatg gagcagcgtc
     6601 taccgtcagc tggtcgccgc cggatacatc aacgtggaca tggaagccta cggcgggctg
     6661 aaattatccg aggccgcacg ccccgtgctg aaaggtttgc aggaagtgtg gctgcgccgc
     6721 gatgccgacc cggtcaaacg caagtcgctg cgtgccgaag gcaaagcgaa gtcacgcgaa
     6781 cctttcgccg gtgcgagcga tgatgtcctg tggctcgcgc tcaaggccaa acgcacggaa
     6841 ctggcgcgcg aacagggcgt gcctccctac gtcatcttcc acgacagcac gctgctggaa
     6901 atccataacc atcgcccgca cacgctggac gagatgggca agatcagcgg cgtgggacag
     6961 gccaagctgg caaagtatgg cgatgccttc ctgcaggtgg tggaggatgt cgcgaatggc
     7021 gtgtaggtgc cgggcaaagc agtgcgccgg gattgatgca tcccggtgct tgaggcaaga
     7081 tgcgggctcg gcgtttttta aatgaatcgt gtaatcatca aagaagagaa tagctatggg
     7141 tgctcagtgg aaagccagac ataaagaagt cgcagcgaat gcgaaaggca aaatattcgg
     7201 caagcttgcc aaagagatca tggtcgccgc aaaaagcggg gccgatccgg atttgaattc
     7261 gcgtctgcgt ctggtggtcg aacaggcgaa aaaggcttcc atgcccaaag aaacgctgga
     7321 tcgcgccatc aaaaagggcg caggtctgct agacggcggt gcgcaactgg aacggctggt
     7381 ctatgagggc tttgcgccgc accgtgtccc cgtcatcgtc gagtgcctgt ccgacaatat
     7441 caaccgcacc atgtccagca tgcgcgtgct gttccgcaag gggcagctcg gtgcggaagg
     7501 ctccgtctcg tgggacttca gctatgtcgg catgatcgaa gcaacaccga actccaaaga
     7561 gtccgacccg gaactggctg ccatcgaagc gggcgcacaa gattttgagc cttccgaaga
     7621 aggcgcgacc ctgttcatca ccgatgtcac cgatctggat gcggtaagca aggcattgcc
     7681 ggagcagggt tttactgtca tttccgcaca gctgggttat cgcccgaaga atcctgtcac
     7741 cctcgccagc gatgccgaac gcgaagaagt ggaagcgttc ctggaggcga tggacgggga
     7801 tgacgacgtg cagaacgtct atgtgggcct ggcagggtaa tggaaaagga attttggctg
     7861 acgcgctggg agcgggccga gatcggtttt catcaggacg aggtcaatcc ctacttgtgt
     7921 cagtactggc cggagctgga tcggcacccg ggctgcgagg tgttcgtgcc gctgtgcggc
     7981 aagagtctgg acatgctgtg gctgcggcaa caaggtgcca ccgtactggg ggtggagctg
     8041 agttccattg cggtgcaggg tttcttcgcg gaaaacggct acgccccgca gcatctctgc
     8101 agtgagaagt tcgattgctg cgaggccgat ggcatccgca tcctgtgcgg cgacttcttc
     8161 gacctgaaca gggaagacct ggcgcaggta acgacggtat acgaccgcgc ctcgctggtc
     8221 gccttgccgc cggatatgcg cgagcgttac gcgcgccatc tggtgagcat cctgcccgcc
     8281 ggtacgcaaa tcctgctcgt caccttcgac taccctcaat ccgaaatgtc gggccctccg
     8341 tttgcggtta cgcccgacga agtcgatgcg ctctatcgcg atcacgccga gatacgcctg
     8401 ctggcgcagt gggatgtcat ggcacaaaat ccgcgcttcg agaaacgcgg cgtgacacgc
     8461 ttgcaggaaa atatcttcct gctgacgctg aactagggtc gttggagcaa acgacaagtt
     8521 gcatgcctga gatgggtcgg ggatgcagga atattttttg ctatcaggga gaaacaccgt
     8581 gcgcatactt cacaccatga tccgggtcgg cgatctggat cgttccatcg atttctatac
     8641 ccaggtgctg ggcatgaagc tgctgcgccg caaggattat cccgagggca agttcacgct
     8701 ggccttcgtc ggttacggcg aggagagctc gcaggcggtg atcgaactga cccataactg
     8761 ggataccggc agctatgaac tgggcacggg tttcggccat gtcgccatcg aagtcgggga
     8821 tgtctacgcc gcctgcgagg agatcaagcg tcgcggcggg gcggtggtgc gcgaggcggg
     8881 gccgatgaag ggcgggacga cggtcatcgc attcgtgcgt gatccggatg gttacatgat
     8941 cgaactcatc ggcgcctgag ggctagaacc gctcgccgct gcagtctaga agcgttcctg
     9001 aggcatcagg taacgccatt gtccgggcgg taacttggac atggggacgc cgccgatgcg
     9061 gatgcgtttc atggatagca cctgcaaccc gaccttgtcg cacatatgtg cgatagcgcc
     9121 gggctgcacg cccttgaggg cgaagcgcaa gcgggtctcg ttctgccagc tgaccttgat
     9181 cgggggcagc ggttttccgt tgaacgtgag cccgtggttg agcagcttga gtccatcggg
     9241 tgacagttcg cccgcgactt ccacgacata ctcctgttcc accgtggcgg cgtcatcggt
     9301 cagtttgcgc gtcacacgcc agtcctgagt gaataccagc aaaccgcccg cattccgctc
     9361 cagcggtgtg cactgtgtca ggtggaggaa gtgctgtttg agcaggtgga tgccggacgg
     9421 gtcgtcggcc cagtgcgtgt ctgcagtaat acgaagttcg cgtagcgatt cgtttgctcc
     9481 ctctcccgct ggcgggagag ggttggggag aggggcgcgg ccgtcaggcg gctgatgcaa
     9541 caggatggtc accggttcga ccggggtgag gttggcatcg ggatgcagtt cgacccgttg
     9601 ttcagccacc atgaattgcg gctcttcgac gacctggcca tccaccatga cccagccgcc
     9661 cgcgatatac agctcggctt cgcggcgcga gcagggcacc agggcagcca gacgtttggc
     9721 gagacggacg ggggtggtca taagcgttgt gttctgaaaa ctgaatgctg cattgtaaac
     9781 gctcaggcct gactatcccc ggtagaatcc ggttatggac gccaataata aacctgtaag
     9841 tctgccggac gggatcaccc tcgaagcggc cctcgtctat ctcttcgagc ggatgggctg
     9901 ggagagcatg gccgcagccg tgccgatacg ctgtttcagc cacgatccca gcatcaaatc
     9961 cagtttgaag ttcctgcgcc gcacgccctg ggcgcgagcc aaggtggagc agttgtattt
    10021 gcagaaaatc gaccgctagc caggtttcat tttcgaagcg caaaaaaatg cccccagtcc
    10081 tgtggagtgg gggcggaaga tcccggccat taagaagggt gccgggatta cacgttcagg
    10141 gaggagaaac gtgttttggc ttgcgccaat gcagacatag aggggcagtt tccggaatag
    10201 ttccagaaag attgaaattt ttttcattcg aatgaaaaag ccccgtattc cggggctctg
    10261 aagtgaaaag ggcggccagc cgcccgatcc gtttgcgtga agcgagttat ttcccggcta
    10321 cgcgctccgc cgcctgcacg gtgttcgcca ccagcatcgt gatggtcatc gggccgacgc
    10381 cgccgggtac cggagtgatc cagcccgcaa cttccttggc cgagtcgaaa tccacgtcgc
    10441 cgcacagctt gccgtccgcc atgcggttga tgcccacgtc gatcaccgct gcccccggct
    10501 tgatcatgtc gccggtgatc atcttcggct tgccggtggc caccaccaga atgtcggcgt
    10561 cgcgggtgaa tttggcgaga tcgacggtct tcgaggtgca gatggtcacg gtggcattgt
    10621 gctgcaacag cagcagcgcc atcggcttgc cgacgatgtt gctgcggccg accaccacgg
    10681 catgcttgcc ctcgatggtc acgccgctct tctgcagcag gatcagcgaa ccgtaagggg
    10741 tgcagggcgc gaagcgcatg ttgccggtgg ccagcgcgcc tacgttcatc gggtggaagc
    10801 cgtccacgtc cttgtccggg ctgatcgcgt tcagcacctt gtcgccgtcg atgtgcttgg
    10861 gcaccggcag ctgcaccagc aggccgtgga tcttcgggtc ggcattcagc tcggcgatct
    10921 tcgccagcaa gtccgcctcg gaagtgtcgg ccgacagggc gatgtgctcg gaatgcaggc
    10981 ccagctccgc acaggccttc accttgttgg cgacgtacac cttggaggcc gggtcttcgc
    11041 cgacgatgat gactgccagc cccggcgtga tgccgcgtgc cttcagtgca tcggcacgca
    11101 ccttccattc ggcgcggact tcctgcgcga ttgctttgcc gtcgatgata cgtgcggtca
    11161 tgttcgcctc ctggtatggg ttacttgttt aaggaagcct gataggccgc gagaccattg
    11221 gtgatttcct tgtgggcggc ttccacccct tcccagccac ggatcttgac ccacttgccg
    11281 ggttccagcg ccttgtacag ttcgaagaag tgcttgatct gctccagcgt gatctccggc
    11341 aggtcgctgt aggttttgac gtcgcggtac agcggcgtca gtttgtcggt tgggaccgcg
    11401 atgatcttgg cgtcgtcgcc gccttcgtcg gacatcatca gcacgccgac cggacggcag
    11461 gcgatgatcg cgccgtgcac gatagggaag ggggtaacca ccagtacgtc caccggatcg
    11521 ccgtcgtcgg cgatggtctc ggggatgaag ccatagttgg ccgggtagcg catcagcgag
    11581 ccgatgaaac gatcgaccac cagcacgccc gcatccttgt ctacttcgta cttgaccggg
    11641 tcggcgtgtt tagggatctc gatgatgaca ttgcattcat gtggcagctt cttgccggga
    11701 cggacgttta acaggctcat gcgacttcct taaagttaat cagataaaga ctacgcaatt
    11761 ataagcgatg ccggacgatg ctgctaagcg cattaggttt agaatggttc tccttcactc
    11821 atttcaggag atggtgccat ggcgacgcag aacaaattga tcggaaaaat ggtcatcgga
    11881 caatcgggcg gccccaccgc cgtcatcaat cagtcgctgg tcggcgcggt gctggccgca
    11941 cgcggacagg ccaatatcac cggcatcctc ggcgcgcgtc acggcattgc gggcatcatg
    12001 caggaggact tcatcgacct caccacccag agcgtcgaac aactggaact ggtcgcgacc
    12061 acgccggcgg cggcgctggg ttcggtgcgc ctcaagcccg gccgcgccga gtgcgagaag
    12121 gtgttcgagg tgttcaaaaa aaacgacgtg cgttattttt tctacatcgg cggcaacgat
    12181 tctgccgaga ccgctcatat catcgccgag atggctaaag aagctaacta cgatttctgc
    12241 acggtgcata tccccaagac catcgacaat gatctgaagg tcaccgacca ttgccccggc
    12301 tacgcctccg ccgcgcgctt cgtcgcgctg gccttcatgg gcgacgaccg cgacaaccgc
    12361 gccttggcgg gtgtgaaggt caacgtggtg atggggcgca gcgccggatt cctgacggcg
    12421 gcgtccgcct tggcgcgtca agccgagggc gacgggccgc atctgatcta tctgccggaa
    12481 cgggtgttcg atgtggcgca gttcaagcag gatgtgcgcg acgcgatggc gaaatacggg
    12541 cgctgcgtgg tcgcggcatc cgaaggcatc tccgacaagg acggcaaccc gatctcgacc
    12601 agcggtgaaa aagattcaca cggcaacgtt cagttgtcgg ggtcgggtgc gctgggcgat
    12661 acgctcgccg cgctggtaaa agaggcctac gcgggacaga aggtgcgggt acgagccgac
    12721 accttcggct atctgcaacg ctccttcccg accatcgtct cggagattga tgccagggaa
    12781 gcgcgcgcag tgggcgacta tgcggtgaag catgccaccg gcaccggact gcccggctcg
    12841 gtggcgatac gccgcctctc cagcaagcct tatgccagcg aatgtttcat cacgccgctg
    12901 tcctcggtcg cgcgcgaggc gaccaagatg aaggacgaat acatcaatgc ggcaggcaac
    12961 gacgtgacgc aggcgtggct ggattacgtc gcgccgctgg tgggtgagtt gccgaagatg
    13021 ggacgcctgt agaaacgaaa caaatcaatc agtccacgaa aagcacgaaa gacacaaaaa
    13081 aatcaaaaga tactttctcc agttcaagaa atatcctgag gtttttattt cgtgagtttc
    13141 gtgtctttcg tggacatctg ttttttctgt cttattcctg agtccaggcc agcgcttcgt
    13201 cgtagtcggc aaacgcgcgg gtgttcgcat ccacgaacag gcgggacagc caggcatgcc
    13261 aggtgatcca ctggtcgtcg gtgacgacgg cgatcttgtt gaagtcgtga ttgtgctcgc
    13321 ggttgaagaa tttgatctcc tcccaggcga cgtcgatggt gtagtcgacc atgccgcgca
    13381 ggtcgaacag caggttgatc gggccggtcg atttcaattt atgcagcgac tcttcttcaa
    13441 attttttgaa gtcggcgagg gtgaattcgc cgatgacggc ggcgttgacg aggtgctcgg
    13501 tctgttcgat ggtgatcatg gttatgtttc ctgatgcttg ggtgacaggc gcaagcttag
    13561 cacaccgctg gcgacgatga gcgccatgcc tagccaactg ctggccgtta aagtttcatt
    13621 ccacagcaac atgccgaaca ggctggcaaa cagaatggtg ctgtaggcca tgctgccgac
    13681 gaccagcgtc ttgccgacct gataggcgcg ggtcatggcg agctgggcga gggtggccgt
    13741 gccgcccaag cccagcaaaa tgagcaagcc ttgcgctgtg acggcatgca cctcgtcgaa
    13801 cagcatccag gcgccgctgc cgatggaggc gatcaggctg aagtagaaca cgatgcgcgt
    13861 cgccggttcg ccctgcatcc cgagttgttt gacgttgaga taggcgatgc cggcgaggaa
    13921 gccggaaatc agaccgagca gtcccagcca caactggtcg cgttccagcg tcgggtgcag
    13981 caacagcacc acgccgcaga agcccagtgc gatggtgcag gtcagcggca gattgaaacg
    14041 atccttgaac accagcatcg tgaagatggt gaggaacagc ggtgcggtgt aattgagcgt
    14101 gaccgccgtg gcgagcggca acatggtgat gcagtagaag aacagcgcca gagccacggt
    14161 gcccgataga cctcgccaca aatgcggttg ccagtgtttt gtggcgaggc tcacgcgttg
    14221 ttgacgcagg atcgcataga ccagcagcag gccgaagaac gagcggtaga acaccagttc
    14281 gatatgggaa aaatgcgtcg cccccagctt gacgaacacg cccatgcagc cgaacaggaa
    14341 tccggccaac agcatccaga ggctgccagc ttgcttatgc acggcgagcg atatcggcca
    14401 gcctgttcag atcgcatttc atgatcgtgg cccgctcgcc cagataatcc tcttcccgct
    14461 ggtattccca tcccaggctt tcgtagaggg ggcgggctga gacggtgtac agccataatc
    14521 ggctcacacc cagtcgtgcg gcttcatgca tcgcgtgtcg ggatagtgcg gtggcgatgc
    14581 cgcgcccacg gtgttccggt ttgacatata cgccgcccag ccacgggctg atctccgggt
    14641 gggtggtcat gtcgtcgttc accagcatgg ccgtgccgat cggctggccg ttttcgatgg
    14701 cgacatagac cgtcgggatg cggtccttgt tggtcgactg acgcacctgc tccgcccact
    14761 cctcgcccca ttcccgccgg tgccagtcgc atatgctgtc gagcagatcc ggacggctgg
    14821 agatgttttc gatcaggaac gtcggcgtca tgtggcgcaa cgggtgcaag tgcattgttc
    14881 attcgtctgc ctggactgca gctctgccaa tgcctccaga gtgaggtcat ggcggcgcag
    14941 gacgaagcgg ttcgaggact ccgtgtctgc gcgggtctcg cgggcaaggt tttcgattcg
    15001 ttccctggtt cgggtcgaga tgccatgcag gttgcagttg aagacaccga tgaaatcatc
    15061 gtcgtacctg tagccctcct ggacgaggaa atagcgcagc gagggactgg tcagatagat
    15121 gccgcacatg tagcgaaccc atatggcgct gacttcgtta ttgagggagt tctgcgatga
    15181 gtagaagctc tcgaattgat cgcacatgat ctcgcaggcc gtcatcaccc ggtcccatgc
    15241 gccatcgtca gggaactttt ccggcgacgg tttgcagtcg tagaagtagg ggcgcaactc
    15301 atggttttcg atgaacagct tcaggacgtc gatgcccgat ccgtagacct gtgcactggt
    15361 ctgtacttcc agcacttgtt gctcccgccg gagttgatag cccagaaaca ggaagccggc
    15421 aatcgtcgcc agcgcgctgg tccaatcgcc agcactgccg atggcgttcc agtcgttgtt
    15481 cacggaagcg ggggaggcgg aaaggaacag tgtgcccagc agcagcagct tgtagaggaa
    15541 gaagagtatg cccagcgcta gaacaacccc gagcatccag atatggcgaa gtaaaaatcg
    15601 cccgatccgc caataagtcg tattgatatt tttgtgcatt gcgccagtcg tcatgttgaa
    15661 attgccgtgg atcttcagtc gctccgcgta caagtttaaa tcattcatgc ctgtttcaac
    15721 attagatcga tgacggattc gggttaatgg ctgccggata tcggcgctcg aacagtcaag
    15781 caggtctaag cccctgagat tctccccgtc aggtgcgcag gctgcctatg ctgagggttg
    15841 catcagtcgt cacgttgtgt gctatctttc ccgccaccta aaccgatgag cttttgatgt
    15901 catgagcctg tttgccctga tcgcggcact gttgctggaa caactgcacc cgctctctgc
    15961 gcggaaatat ctgtatggct ggctgaccgg ctatgccaat ttttatcagc tccatttcaa
    16021 tgcgggcgag ctgcgccacg gcaagttcgc ctggctgttc gccgtgctgc cattgctgtt
    16081 cggcacgatc ctgctgtact ggctgttgta ccgcgtccat cccttcttag cctgggcgtt
    16141 caacgtgctg gtgctgtatc tcaccatggg ctttcgccag ttcagccact acttcaccga
    16201 catccacaag gcattgcgcg acggagagct ggagcgggcg cgcggtctgc tgacctcgtg
    16261 gcgcggtgaa ccggcgcacg aactgaatgc ggaagaggtg gcgcgtgtca ccatcgaaga
    16321 ggcgatgctg gcctcgcacc gcaacgtgtt cggtgtgatc gtctggttcg tgttgttcag
    16381 cgtgctgggg ctgggcggtg cggccggcgc cttgctgtat cgcatggggc agtttctgcg
    16441 ggcgcgctgg ggcgacgagg aagacgacga tctggagctg ttcggcaatt tctcgcgcaa
    16501 ggcgttccat gtgctggaat ggctgcctat ccgtttgacg gcgatgacct tcgccatcgt
    16561 cggcgacttc gaagataccg tgtattgctg gcgcactcag gcggcaagct ggccggatgg
    16621 ggatgacggc atcctgctgg ccagcggtgc gggtgccttg ggggttcgcc tcggcatgcc
    16681 gatcccgcag ggggggctgc cgttcgaccg gccggaactg ggcgtgggtg aggatgccga
    16741 tgccgacttc atgcaaagca ccatcgggct ggtgtggcgc tcggtggtgt tctggctgtt
    16801 gctgttgctg ctgctgacgc tggcgagcct gctgggttga gcaggcccga gaccatgatt
    16861 cattccctca tcccgatcaa gcatcccccg gttcgtcagg cagaaaaatg agcagagaaa
    16921 gcatcgtcgg caagctgaaa tcggcgttgg acaatcttga acggttgccc gcgatgccgg
    16981 ctatcgcaca caagttgctg gcgctgccgc tggataccga tgccggcgaa gcgcagctga
    17041 tcgttctgat cgaacaggat ccgcagctgt ccgccaaaat tctcggcctg gcgaattctc
    17101 ccgtcatggg ggtaggccgc aaggtggccg gcatcaagga tgcggcgatg ctgctcggtt
    17161 ttaaacggtt gaagtcggtg gctatcggca tcgcgaccat gtcgagattc accaatcagc
    17221 ctgcggtgaa ga
//
LOCUS       DASUIV010000068         4082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_946090, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000068 DASUIV010000000
VERSION     DASUIV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946090"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /gene="nifB"
                     /locus_tag="VFW59_02565"
     CDS             complement(<1..818)
                     /gene="nifB"
                     /locus_tag="VFW59_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HEX5363127.1"
                     /translation="MELPVLNNTASESPKGGCSAGSCGSTDDQMNHLPEHIRQKVFNH
                     PCYSEEAHHYFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELHHPVQAVKKVL
                     AVAATIPQMTVLGIAGPGDPLANPERTFETFRMLSEQAPDIKLCVSTNGLTLPEHVEE
                     LSRYNIDHVTITINCVDPDVGAHIYPWIVWKNKRIKGREAAAILIEQQQKGLEMLVAK
                     GVLVKVNSVMIPGVNDKHLEEVSKIVKAKGAFLHNVMPLISEAEHGTYYGLTGQR"
     gene            1151..2503
                     /locus_tag="VFW59_02570"
     CDS             1151..2503
                     /locus_tag="VFW59_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5363128.1"
                     /translation="MKHHLDWSSYETAGQGDAYAGIPATGGDFAKAVAVCISDKQCQR
                     KPKGVMCPSFRATDDVAHSPGGRVQVLKAALNGELGEDAFAGAPLAEALELCVGCKGC
                     KRECANQVDMAAIKIEYQAQRNARLGVPLRERLVAGLPAWLRHRRALRSLIRWRNRAP
                     WLAKLGERWLGISARRQLPEPAAVPYALSVAASEASDREIVLFVDTFAMHFEPQIAEA
                     AHEVLTAAGYHVTILRAAADDEPERALCCGRTYLSLGQVDAARGEARRLQQALRPALA
                     AGTPIVGLEPSCILSLRDDHLKLGLGDEAIALSKQVYLFEEFIAREHDRKRFILALKP
                     ITRGRTLVHGHCHQKAVGAMKSLRKVLRLIPEFDFEFIEASCCGMAGSFGLEAEHADI
                     SMRMAELDLLPALRAEPEATILANGFSCRHQIREGLARQPVHVALLLREALDKGAQPC
                     "
     gene            2497..2847
                     /locus_tag="VFW59_02575"
     CDS             2497..2847
                     /locus_tag="VFW59_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363129.1"
                     /translation="MLTGAMLGLMEQLGLDILTLTEALDEAEFFTSRLTRAQTLKILG
                     SMARTAAELPAGTREILPLIDWGAWAVLNKALANPSQHPLLIWVAIKELTPVTVQQLA
                     DYKRTQPQLFAMVI"
     gene            complement(2933..3157)
                     /locus_tag="VFW59_02580"
     CDS             complement(2933..3157)
                     /locus_tag="VFW59_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363130.1"
                     /translation="MNTFTPCKGKTACQDDGASCLTCGRSFAEIEQTRALIDALADFA
                     CVQGYENIGEFADYVADKVVNKVKHRRSIA"
     gene            complement(3154..3462)
                     /locus_tag="VFW59_02585"
     CDS             complement(3154..3462)
                     /locus_tag="VFW59_02585"
                     /inference="COORDINATES: protein motif:HMM:NF039672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6156 family protein"
                     /protein_id="HEX5363131.1"
                     /translation="MTSTTVENRGAPRFFVTYTGIKLPFRLIDELQASAVENRNTYFR
                     GYFDARQRITGFDKLAYGEVELTHRYSYHDNGQLQQAEINDIDGETTVLEFDADGTPA
                     "
     gene            complement(3468..3686)
                     /locus_tag="VFW59_02590"
     CDS             complement(3468..3686)
                     /locus_tag="VFW59_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HEX5363132.1"
                     /translation="MAIKDIKAFADKARSDAELKEKLAACQKVRELLTLAKENGFSFI
                     EDEMYPPNEPQFTADQLSERLAKALLRA"
     gene            complement(3829..>4082)
                     /locus_tag="VFW59_02595"
     CDS             complement(3829..>4082)
                     /locus_tag="VFW59_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5363133.1"
                     /translation="NRLPLEQLKIDQTFIRNIGHDKNTRIMVRTIINIANNFGLQIVA
                     EGVETDEQLAFLRQYGCNRYQGYLFGKPVPAEEFESLCH"
BASE COUNT          736 a         1173 c         1305 g          868 t
ORIGIN      
        1 cgctggccgg tgagaccgta gtaggtgccg tgctcggctt ccgagatcag cggcatcacg
       61 ttgtgcagga acgcgccctt ggctttgacg atcttggaga cttcttccag gtgcttgtcg
      121 ttcacgccgg ggatcatcac cgagttcact ttcaccagta cgcctttggc gaccagcatc
      181 tccagcccct tctgttgttg ctcgatgagg atggcagccg cctcgcgacc cttgatgcgc
      241 ttgttcttcc agacgatcca tggatagata tgcgcaccga cgtcgggatc gacgcagttg
      301 atggtgatgg tcacgtggtc gatgttgtac ctggacagtt cttccacatg ctcgggcagg
      361 gtcaggccgt tggtcgatac gcacagcttg atgtccggtg cctgctcgga cagcatgcgg
      421 aacgtctcga aggtgcgttc cgggttggcg agcggatcgc ctgggccggc gatgcccagc
      481 accgtcatct gcgggatcgt cgcggcaacg gccagcacct tcttcaccgc ctgcacggga
      541 tggtgcagtt cggacaccac gcccggacgc gactcgttgg cgcaatcgta cttgcggttg
      601 cagtagttgc actggatgtt gcaggccggt gcaacggcca cgtgcatgcg tgcaaagtag
      661 tgatgcgctt cttcggagta gcaggggtgg ttgaacacct tctggcggat gtgctccggc
      721 aggtggttca tctggtcgtc ggtcgagccg cacgacccgg cggaacatcc gcccttgggc
      781 gactcgctcg ctgtgttgtt caagactggc agttccacgt tttgtcctcc ggtaaggtct
      841 gccagaacat gagcaaacag cgtgcccgcc tggtaatttt cgactaagtc attgttttaa
      901 tttggatggc gtgttttcct ctttctcaac tgttgcaaac gacacatgac agcggacagg
      961 gcttgtggcg aagccgacaa aaccgggaga cgcgttgttc tgcggggaag ggtagggcga
     1021 tccatggcag ggttcgctgg ccgtatcagc cttgccatca atgagttatg gatcatgagc
     1081 ggcggctatt cctgactggc actctatttg cactctgtca tcagcatctg cagtcggagc
     1141 gcattcgtac atgaaacatc atcttgattg gtcgtcctat gaaacggcag ggcagggcga
     1201 cgcctatgcc ggcatccccg cgacgggcgg cgacttcgcc aaggccgtcg cggtgtgcat
     1261 cagcgacaaa caatgtcagc gcaaacccaa gggtgtgatg tgtccgagct ttcgtgctac
     1321 cgatgacgtc gcacattcgc cgggcggcag agtgcaggtg ctcaaggccg cgctcaacgg
     1381 cgagcttggg gaggacgcct tcgccggagc gccactcgcc gaggcgctgg aactgtgtgt
     1441 cggttgcaag ggctgtaagc gcgagtgcgc caaccaggtc gacatggccg cgatcaagat
     1501 cgagtatcag gcgcaacgca atgcgcggct gggcgtgccg ttgcgcgagc gactggtggc
     1561 cggattgccg gcgtggctac ggcaccgcag ggccttgcgc agcctgatca ggtggcgcaa
     1621 tcgcgcgccc tggctggcga agctcggcga acgctggctg ggtatctcgg ccaggcgtca
     1681 gttgcccgaa cccgctgcgg ttccctatgc cttgtccgtc gctgcgagtg aggcgagcga
     1741 cagggagatc gtcctgttcg tcgatacctt cgcgatgcac ttcgagccgc agatcgccga
     1801 ggccgcccac gaggtgctga ctgcggcggg ctatcacgtc accatcctgc gggctgctgc
     1861 agacgatgaa cccgaacgcg ccttgtgttg cggccgcact tacctttctt tagggcaggt
     1921 cgatgcggcg cgaggcgagg cccgacgtct gcaacaggcg ttgcgtccgg cactggctgc
     1981 gggcacgccc atcgtcggac tcgaaccctc gtgcatcctg tccttgcgcg acgaccacct
     2041 gaaactcggt ctgggggatg aggccatcgc cctgtccaag caggtctatc tgttcgagga
     2101 gttcatcgct cgcgaacacg accgcaagcg cttcattctg gcactcaaac ccatcacgcg
     2161 cggcaggaca ctggtgcacg gacactgcca ccagaaggcc gtcggcgcga tgaagtcgct
     2221 gcgcaaggta ctgcgcctga tccccgaatt cgatttcgaa ttcatcgagg ccagttgctg
     2281 cggcatggcc ggaagtttcg ggctggaagc cgagcatgcc gacatctcga tgcggatggc
     2341 cgaactcgac ctgttgcccg cgttgcgcgc tgaacctgag gcgaccatcc tcgccaacgg
     2401 tttctcctgc cgtcaccaga tccgtgaagg gctggcgcgg caacccgtac acgtcgcgct
     2461 gctgctgcgc gaggcgctgg acaagggcgc gcagccgtgc tgaccggtgc gatgctgggt
     2521 ctgatggagc aactcgggct ggatatcctg acgctgacgg aagcgctcga tgaagcggag
     2581 ttcttcacca gccgcctgac gcgtgctcag accttgaaaa ttctgggcag catggcacgg
     2641 accgcagctg agctgcccgc cggaacacgt gagattctgc cgctgatcga ctggggcgcc
     2701 tgggcggtat tgaacaaggc gctggcgaac ccgtcgcagc accccttgct gatatgggtc
     2761 gcgatcaagg aactgacgcc ggtgaccgtg cagcaactgg ccgactacaa gcgcacacag
     2821 ccgcagttgt tcgctatggt gatctgagcg gccagcgata tcgaacggtc aacgagcggg
     2881 aggaaaaagc gagacgccgg gcatatgcaa gatgcgcagc agttccatgc ggtcaggcga
     2941 tagaccgccg gtgtttcacc ttattcacca ccttgtcggc aacatagtca gcgaactcgc
     3001 cgatattctc gtagccttgg acgcaggcga agtcggccag tgcatcgatc aatgcgcggg
     3061 tctgttcgat ctcggcgaag ctgcgtccgc aggtcaggca gctggcgcca tcgtcctggc
     3121 aggccgtctt gcctttgcag ggagtgaagg tgttcatgcc ggggtaccgt ccgcgtcgaa
     3181 ttcaagtacg gtggtttcgc catcgatgtc gttgatctcc gcctgctgga gttgaccgtt
     3241 gtcgtgatag ctgtagcggt gtgtcagttc gacttcgcca taggccagtt tgtcaaaacc
     3301 ggtgatgcgt tgccgtgcat cgaaatagcc acgaaagtag gtgttgcggt tctcgacggc
     3361 gctggcctgc agttcgtcga tgagacggaa cggcagcttg atgccggtat aggtgacgaa
     3421 aaaccggggg gccccccggt tctccacggt ggtggaagtc atgacgatca ggctcgcagc
     3481 agggctttgg caagccgctc ggaaagctga tcggcggtga attgcggctc gttgggtggg
     3541 tacatctcat cctcgatgaa gctgaacccg ttctcttttg ccagcgtgag cagttcgcgc
     3601 accttctggc acgcggccag tttttccttc agttcagcgt cgctccttgc cttgtcggcg
     3661 aaggccttga tgtctttgat agccatgtgt atctctctct ttcagaattg aacaaagccg
     3721 ggtcgtagaa cgatccgaca gcttgccggg ctggatatcc agtccggcag acagacgagc
     3781 aagttgcatg cctgagttgt gagcggcgat gttcgagtgc caccgccgtc aatggcagag
     3841 actttcgaac tcttccgcag ggaccggttt cccgaacaga taaccctgat agcggttgca
     3901 tccgtactgg cgcaggaagg caagctgctc gtccgtctcg accccttcgg caacgatctg
     3961 caggccgaag ttgttggcga tgttgatgat ggtgcgcacc atgatgcggg tgtttttgtc
     4021 gtgaccgatg ttgcggatga aggtctggtc gatcttgagt tgctccagtg gcaatcggtt
     4081 ca
//
LOCUS       DASUIV010000069         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1226892, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000069 DASUIV010000000
VERSION     DASUIV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226892"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="VFW59_02600"
     CDS             <1..2147
                     /locus_tag="VFW59_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5363134.1"
                     /translation="EPRNVTVYFSVCPGYEETLAKVIVSLTDITERKRAEEAQQRLNR
                     ELRAISDCNQTLMRADNEQSLLDSICRIICDEAGYRMAWVGFAENDAAKTVRPVAWAG
                     AEEGYLELGGFTWADTELGQGPCGIAIRSGNAACIEDFISDPQVAPWRDNALQRDYRS
                     SIALPLKDESANTFGVLNIYSDQPNTFTADEKRLLEELAGDLAFGIAALRTRTERKLV
                     EEELRRYRDQLEDTVQQRTAELLLARNAADAANKAKSLFLANMSHELRTPLNAILGFS
                     GMMRRDPQLTDSQRENLDIINRSGEHLLTLINDVLEIAKIEAGRLQLEVAPLDLGGMV
                     RDVTEMMQLRAQEKGLRLQIDQTSEFPRFIRGDEARLRQILVNLVGNAVKFTAQGGVT
                     IRLGIKNNARQHLLIEVADTGPGISPEDQKRLFEPFVQLTEGTAQHGTGLGLSITRQF
                     VQLMGGNIAIDSAPGKGARFRVDLPLELASAADMLELENRTHGEIAGLAPGQPRYRIL
                     IAEDQHENQLLLARLMSDIGLEVKVAEDGVKCIQIFRDWHPDLIWMDKLMPVMDGVEA
                     TRRIRQLPDGQKVKIVAVTASAFKEQLPELLDAGMNECVSKPYRFNEIYDSLARQLDV
                     KYIYQSGETKELPPVALTPDKLLVLPVDLRLELQHALENLDSERIAASIHRVGEIDAE
                     LGRTLSRLAEYFDYPAILNALAEAALSKGGTP"
     gene            2144..>3147
                     /locus_tag="VFW59_02605"
     CDS             2144..>3147
                     /locus_tag="VFW59_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5363135.1"
                     /translation="MTNKGNILAVDDSPASLKLLTDLLKEDGYEVRSAISGELALRAA
                     SVLPPELVLLDVSMPGLDGFEVCRRLKEQEQTRDVPVIFVSALSETVEKVKGFELGAV
                     DFVTKPYQRDELLARVRTHLELYRLRHHLESLVEERTTELRESEKKLRINLLDSVTAL
                     AAIVEMRDPYTAGHQRRVAQLAVAIAKELNLADHMIEGIHLAGVVHDVGKIQVPAEIL
                     SKPGRLTELEYRLIQEHVQSGYEILKTIEFPWPIAQILRQHHERLDGSGYPQGLVGEQ
                     ILPEAKIMAVADVVESMISHRPYRPGLGLDAALAEIEANRDKLFDPAAVNACIKLFRE
                     R"
BASE COUNT          700 a          982 c          956 g          509 t
ORIGIN      
        1 gcgagccgcg caacgtgacc gtatatttct cggtttgccc gggttatgag gagacacttg
       61 caaaagtcat cgtgtccctg accgacatca ccgaacgcaa gcgagccgaa gaggcgcagc
      121 agcggctgaa ccgcgaactg cgcgccatca gcgattgcaa ccagaccctg atgcgcgccg
      181 ataacgaaca gtccctgctc gactcgatct gccgcatcat ctgcgacgag gccggctacc
      241 gcatggcctg ggtggggttc gccgaaaacg atgcggcaaa aaccgtacgt ccggtcgctt
      301 gggcaggcgc ggaagaaggg tatctcgaac tcggcgggtt cacttgggcc gacacggaac
      361 ttgggcaagg cccctgcggg atcgccatcc gcagcgggaa tgcggcctgc atcgaagact
      421 tcatatccga tccgcaggtc gctccctggc gggacaacgc cttgcagcgc gactaccgct
      481 ccagcatcgc tctcccgctc aaggatgaaa gtgcaaacac gttcggcgtg ctcaacatct
      541 attccgacca gcccaacact ttcacggcgg acgagaagcg gctgctggaa gagctggcgg
      601 gcgatctggc cttcggcatt gccgccctgc gcacccgcac cgagcgcaag ctggtggaag
      661 aggaactccg ccgctacagg gatcaactgg aagatacggt gcagcaacgc acggcggaac
      721 tgctgctggc gcgcaatgcg gccgatgcgg cgaacaaggc caagagcctg ttcctcgcca
      781 atatgagcca cgaactgcgc accccgctga acgcgattct cggcttctcc ggcatgatgc
      841 gccgcgaccc gcaattgacc gacagccagc gcgaaaatct cgacatcatc aaccgcagcg
      901 gcgaacacct gctgaccctc atcaacgatg tgctggagat cgccaagatc gaagcgggcc
      961 gcttgcagct cgaagtcgcg ccgctcgatc tcggcggcat ggtgcgggat gtcaccgaga
     1021 tgatgcagtt gcgtgcgcag gaaaaaggct tgcggctcca gatcgaccag acctccgagt
     1081 tcccgcgctt catcaggggc gacgaggcgc ggctgcgcca gattctggtc aacctggtcg
     1141 gcaacgcggt gaaattcacc gcgcagggcg gcgtgaccat ccgcttgggc ataaagaaca
     1201 atgcacggca acacctgctg attgaagtcg ccgacaccgg ccccggcatt agccccgaag
     1261 accagaagcg gcttttcgag ccattcgtgc agttgacgga gggaactgcg cagcacggca
     1321 ccggcctggg actcagcatt acccgccagt tcgtgcaact gatgggcgga aacatagcca
     1381 tcgacagcgc gccgggcaag ggggcgcggt tccgggtcga tctgccgctg gagctggcca
     1441 gcgcagccga tatgctcgag ctggaaaacc ggacgcacgg cgagattgcg ggactcgccc
     1501 ccggccaacc gcgctaccgt atcctgattg ccgaagatca gcacgagaac caactgctgc
     1561 tcgcccggct gatgtccgac atcggcctcg aagtgaaggt tgcggaagac ggtgtcaaat
     1621 gcatacaaat tttccgcgac tggcatccgg acttgatctg gatggacaag ctcatgccgg
     1681 ttatggacgg cgtggaggca acccggcgca tccgccaact gcccgacggg cagaaggtca
     1741 agatcgttgc ggtgacggcc tccgcgttca aggaacaact gccggaattg ctcgatgcgg
     1801 gaatgaacga atgcgtgagc aagccctacc gcttcaacga aatctacgac agcctggccc
     1861 ggcaactgga cgtaaaatac atctaccaat ccggcgaaac caaagagctc cctcccgtcg
     1921 ctctgacgcc agacaagctg ctggtattgc cggtcgatct gcgattggaa ctccagcatg
     1981 cgctggaaaa tctggacagc gaacgcatcg cggcgagcat tcaccgggtc ggcgaaatcg
     2041 atgcggaact gggacgcacg ctgtcccgtc tcgccgaata tttcgactac ccggccatcc
     2101 tgaatgcgct ggccgaggca gccctctcga aaggtggcac accatgacaa acaagggaaa
     2161 catactcgcc gtcgatgatt cgccggcatc gctcaagctg ttgacggacc tcctgaaaga
     2221 ggatggctat gaagtgcgct ccgccatcag cggcgaattg gcgttgcggg ccgcaagcgt
     2281 cctgccgccg gaactggtgc tgctggatgt cagcatgccc ggtctggacg gcttcgaggt
     2341 ctgccgccgc ttgaaagaac aagagcagac ccgcgacgtg ccggtgatct tcgtcagcgc
     2401 gctgtcggaa accgtggaga aggtgaaagg cttcgaactg ggcgcggtgg acttcgtcac
     2461 caagccgtac cagcgcgacg aattgctggc cagagtgcgc acccatctgg agttgtatcg
     2521 cctgcgccac catctggaga gtctggtgga agagcgcacg accgaactgc gggaaagcga
     2581 aaaaaaactg cggatcaacc tgctcgactc agtcaccgcg cttgccgcca tcgtcgaaat
     2641 gcgcgacccc tataccgcag gccatcaacg ccgcgtcgcg caactcgccg tcgccatcgc
     2701 caaagaactg aatctggccg atcacatgat agaggggata catctggcgg gggtcgtaca
     2761 cgatgtcggc aaaattcagg ttccggcgga aatcctcagc aagccgggac ggctcaccga
     2821 actggaatac cggctcatcc aggaacacgt ccagagcggc tacgagatcc tgaaaaccat
     2881 cgaattcccg tggcctatcg cgcaaatcct gcggcagcac cacgaacgcc tggatggttc
     2941 gggatacccg caagggctgg ttggcgagca aatcctgccg gaggccaaaa tcatggcggt
     3001 agccgacgtg gtggaatcga tgatctccca ccgcccctac cgcccgggac tggggctaga
     3061 cgcggcgctg gcggaaatcg aagccaaccg ggacaagctg ttcgaccccg ccgccgtcaa
     3121 tgcctgcatc aagctgttcc gcgaacg
//
LOCUS       DASUIV010000070         7312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_110725, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000070 DASUIV010000000
VERSION     DASUIV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110725"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VFW59_02610"
     CDS             complement(<1..1444)
                     /locus_tag="VFW59_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX5363136.1"
                     /translation="MPAFINTNVASLNAQRNLNASQSALQTSLQRLSSGLRINSAKDD
                     AAGLAISDRMTAQIRGLDQARRNANDGVSLAQTGEGALAGASDMLQRIRELAVQSANA
                     TNSASDRAALQSEAGLLVSELDRVASSTQFNGQNILDGTAGTSIFQVGANANQTINAT
                     TANFRTANYGNFRIGASVANTSNGAGDLTVGSTVGTLPSSATAGTIGRVAAAGSFALD
                     GAAGSAVINYAIGDSAKTVAAAVNAQTGATGVTASARTEIDITTMTAGSSYSLAVTSD
                     NAVATTVSFSVSANNADGLAAAIKAFNDVSSSTGVTAMLNTAGNGLTLTNATGNDISI
                     LSNNASALNVGAVAIAAAGNSAVATGQITLDSDKSFGVSSATAATDFINATTGAAAQL
                     QKVNQIDISSVAGAARTISLVDAALANVSSQRSAFGALQSRFSNAITSLQTTSENLSA
                     ARSRIQDTDFAAETAQLTRNQILQQAGTAML"
     gene            1523..1705
                     /locus_tag="VFW59_02615"
     CDS             1523..1705
                     /locus_tag="VFW59_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363137.1"
                     /translation="MMSIISVSSSFKKPNTDGNPCHFPLLRFHLSVVPASKTGHPVIG
                     REALEIGADSAGVPVK"
     gene            complement(1722..3380)
                     /locus_tag="VFW59_02620"
     CDS             complement(1722..3380)
                     /locus_tag="VFW59_02620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5363138.1"
                     /translation="MSKQAANPSELARETLIKLAAHKTPPTPDNYARIYAEISGQPAP
                     QPSGEQAPSQQEGTSTIPWGGLIGELLRQLDTPHKGLTLTRKKEGLNAVLRNKKFASD
                     PTMLFEKINGLMRLWSESPTAPATTELDSNLDAIPGAAQTPVIPTAPSAPANSDMIGQ
                     LGELLAQALESLFSALPEAIDEIPGIAQQVRAIKTPEQLAALTKQLRQFWIRVELHAN
                     DKSKIHDGLIRLLRLLVENIGEMVEDEEWLHGQVSILQDIIANPIDRRSIADAERGLR
                     DAIIKQSLLKQSLTEAKSTLRILMTNFIDRLGEISSSTGDYHQKIEGYSKKIGQSKNL
                     ANLSHLLDDIMRDTRVIQASALRSHEELLNTRKQVQESETKIRQLEQELNQVSELVRE
                     DQLTGTLNRRGLDAAFEQDAAQSDRAQSPLCVALLDIDNFKRLNDTKGHQAGDLALVH
                     LTGVIKDTLRPSDSVARYGGEEFIILLPDTELLEAAATIERLQRELTKRYFLNENERI
                     LITFSAGVTQRTPQEAQDEVIGRADKAMYQAKQTGKNRVVIAET"
     gene            complement(3426..4067)
                     /locus_tag="VFW59_02625"
     CDS             complement(3426..4067)
                     /locus_tag="VFW59_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5363139.1"
                     /translation="MIDILVVDDHALIRKGLIMLLEENPEFHIKGEAETGMQAINMVR
                     EQHFDLVLLDISLADKHGIDVLKQLKSEQPEIKIIVLSMYPEDQYGVRALKAGAMGYI
                     NKQSAPEKLVGAIHQVISGKKYISPALAEQLLSNLIGESQEQLHQSLSNREYQTLCLM
                     ASGKSLTDISEIMVVSPKTVSVYRARMLEKMGFNNNAEAVHYAIAHDLVEAKK"
     gene            complement(4064..5188)
                     /locus_tag="VFW59_02630"
     CDS             complement(4064..5188)
                     /locus_tag="VFW59_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5363140.1"
                     /translation="MQKAAPNPPTSPSPDKLQGFIASLPGMACQIELQEDGQFTFPYV
                     SEGCQPLLGIAASELQADTTRLLDLIHAEDAPALLESMRTSAQTRCFWNWEGRITLPD
                     GETKWISLGATPQDTQGGAPRWEGIVLNTTQSKLGELEVKRVQQQLLELSSHIQDAKE
                     QERLRIAREVHDEIGSLLTAMRMDLSWLIQRLPKDNPTLTDKAKTIEELVNRAITSAS
                     HLAHRLRPGFLDCFGIIAAIEIEAQEFSKRSGIPCTIIKSQESIELPEAYTITLFRVC
                     QETLNNILKHAQATQVQIEVVKGADHLELIISDDGVGFSEAARDKPHSFGLRGIHERV
                     AHLGGTVKIASTLGLGTQIAVFVPLPEETPQQPLFGGTSI"
     gene            5426..5899
                     /gene="fliW"
                     /locus_tag="VFW59_02635"
     CDS             5426..5899
                     /gene="fliW"
                     /locus_tag="VFW59_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HEX5363141.1"
                     /translation="MADIKVGTRFGELSVDPFQVITFPRGIPGFENSTKWTLFHELDE
                     QGGWASGVVVYLQSLDDGDIALPLTDPTLFGFNFDLVLSDSEIAELQLEDPNDVLVLT
                     TLSLKDPAASKTQRPSMADMYANISAPILINTKSRIGMQKALIARESKVDFHPAA"
     gene            5974..>7312
                     /locus_tag="VFW59_02640"
     CDS             5974..>7312
                     /locus_tag="VFW59_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5363142.1"
                     /translation="MIVPEEVLRHATTFYEAGRLDEAEELCLGVLLTEPEHAAANHYL
                     GLIALQRGLAERALPYLQMAWEVDPSAGAHWLTLAECLLALGRFEDALNLVKEASKRG
                     LDAPQVQQLRLLAKRGLDRTAPSAAVVDEMLALFRAGRNAELESRVSPLLEQYPGWVF
                     GWRALGAAMGMLGRDAEPVLRRAMALAPQDASTHNNLGVVLRERGRQAESLICLRKAV
                     QLSPKYAEAHYNLGTVLQDMSRYDEAIVCFRQAIRIKPDYFEAQAELSNSLSALGRFA
                     EALKILERLPRSRPDHPKALSNLLFNLTHRGDIDADTLFAEHCRFGAQFDAPLQAFAN
                     ARDHERRLRVGIVSGDLHLHAVANFIEPVLLHLRHSAQLSLHAYYNHVQEDEVTQRLK
                     GYFAQWDSVFGLTDEELAQKIRADGIDILIDLSGHTARNRLPLFARKPAPIQAS"
BASE COUNT         1281 a         1950 c         2394 g         1687 t
ORIGIN      
        1 ccaacatcgc agtaccggct tgttgcagga tctggttgcg ggtcagctgt gcagtttcgg
       61 cagcgaagtc ggtatcctga atccggctac gcgcagcgct caagttctcc gaagtggtct
      121 ggaggctggt gatcgcgttg ctgaagcggg actgcaaggc accgaaggcg gaacgctgcg
      181 aactgacgtt tgccagcgcg gcatcgacca gcgagatggt gcgggcggca ccggcgacgc
      241 tggagatgtc gatctggttg accttctgca actgagcggc agcacctgta gtcgcattga
      301 tgaagtcggt agcggccgtt gcagagctca caccgaatga tttgtccgag tcaagggtga
      361 tctggccggt ggccacagcg ctgttgccag ccgcagcaat cgcgaccgct cccacgttca
      421 gtgcggacgc attgttgctg agaatggaga tgtcgttacc agtcgcattg gtcagtgtga
      481 gaccgttgcc ggcggtgttc agcatcgcgg tgacgccggt gctggacgac acatcgttga
      541 atgccttgat tgcggcagcc aggccatcgg cattgtttgc actgacgctg aaggagaccg
      601 tagtggccac cgcattgtcg gaggtaacgg ccagactgta ggacgagcct gctgtcatgg
      661 tggttatgtc gatctcggtc cgggccgagg cggtgacgcc ggtcgcgccg gtctgtgcat
      721 tgaccgcggc ggccacggtc ttggcgctgt cgccaatagc gtagttgatg acggcagaac
      781 cggccgcacc atccagagcg aaggagccag cggcggcaac gcgacctatc gttcctgccg
      841 tggctgagga gggcagcgta ccgacggtgc tgccgacggt caggtcaccc gcaccgttgc
      901 tggtgttggc gacgctggcg ccgatacgga agttgccgta gttggcggtg cggaagttgg
      961 ccgtggtggc gttaatggtc tggttggcgt tcgcgccgac ctggaagatc gaggtgccgg
     1021 cggtgccgtc gaggatgttc tgaccgttga actgcgtgct tgacgcgacg cgatccagct
     1081 cggagaccag caggcctgcc tcggattgca gcgcggcacg gtcactggcg ctgttggtgg
     1141 cgttggcgga ttgcacggcc agttcgcgga tacgctgcag catgtcggaa gcacctgcca
     1201 acgcaccttc accggtctgc gccagcgaaa cgccgtcgtt ggcgttgcgg cgtgcctgat
     1261 cgaggccgcg gatctgtgcg gtcatccggt cggagatcgc caggccagcc gcatcgtcct
     1321 tggcgctgtt gatgcgcaag ccggaagaca ggcgctgcaa ggaggtctgc agggcgctct
     1381 gtgaggcatt caggttgcgt tgtgcattca gcgatgcaac gttggtattg ataaatgctg
     1441 gcatttcgat cctcctaagg taaggtcgtt tcccggtggt tggcttttga actgttgggt
     1501 tcgatagggc caaccagacg acatgatgag cattatcagc gtctcgtcaa gttttaaaaa
     1561 gccgaatacc gatggaaacc catgccattt tccgctcttg cggtttcatc tttcggttgt
     1621 gcccgcatca aaaacgggtc atcccgtcat tggtcgagag gcgctcgaga ttggcgcgga
     1681 ctcggcaggc gtgccggtga aatgaggtta ggcgcgcacc atcaggtttc tgctatgacc
     1741 acgcggttct tgccggtttg tttggcctga tacatggctt tgtcggcgcg accgatcacc
     1801 tcgtcctgag cttcttgcgg agtgcgttgc gttactccgg cgctaaaggt gatcaggatg
     1861 cgttcgttct cattcagaaa gtagcgtttg gtgagttcgc gttgcagccg ttcgatggtc
     1921 gcggcggctt ccaataactc ggtgtcgggc agcaggatga tgaactcttc gccgccatat
     1981 cgggcaacgg agtcgctggg gcgcagggtg tccttgatga cgccggttag gtggacgagc
     2041 gccagatcgc cagcctgatg tcctttggtg tcgttgaggc gcttgaagtt gtcgatgtcc
     2101 agcagggcga cgcagagcgg gctctgggcg cgatcggatt gggctgcatc ctgttcgaag
     2161 gcggcatcga gtccgcgacg attgagtgtt ccggtgagct ggtcctcgcg taccagttcg
     2221 ctgacctgat tgagttcctg ttccaattgc ctgatcttgg tctcggattc ctgaacctgc
     2281 ttgcgggtat tgagcagctc ttcgtgcgaa cgcagggcgc tggcctggat gacgcgcgtg
     2341 tcccgcatga tgtcatccag cagatggctc aggttggcga ggttcttgga ctggccgatc
     2401 ttcttgctat aaccctcgat cttctggtgg taatcgcccg tgctgctgga gatctcgccc
     2461 agacggtcga tgaagttggt catcagaatg cgcaatgtgc tcttggcttc agtcaggctc
     2521 tgcttgagca ggctctgctt gatgatggca tcgcgcaggc cgcgttcggc atcggcgatg
     2581 ctgcgtcggt cgatcgggtt ggcgatgatg tcttgcagga tggaaacttg tccgtgcagc
     2641 cattcttcgt cctcgaccat ctcgccgatg ttttcaacca gcagtcgcag caggcgtatc
     2701 aagccatcgt gaatcttgct cttgtcgttc gcatgcagct ctaccctgat ccagaattgg
     2761 cgtagctgtt tggtcagtgc ggccagttgc tcgggagtct tgatggcgcg cacctgttga
     2821 gcgatgccag gtatttcatc gatggcctcg ggcagggcac tgaacaggct ttccagcgct
     2881 tgtgccagca actcgccaag ctgcccgatc atgtcgctat tggccggggc agatggcgcg
     2941 gtcggaataa ctggagtctg ggcggcgcct ggtatggcat ccagattgga atcaagttcg
     3001 gttgtggcgg gggcggttgg cgattccgac cacaagcgca tcagtccatt gatcttttca
     3061 aacagcatcg tcggatcgga cgcgaacttt ttgtttctta atacggcgtt cagcccttcc
     3121 tttttgcggg tgagggtcag ccccttgtgc ggagtgtcaa gttggcgcaa caactcaccg
     3181 atcaacccgc cccaagggat ggtggacgtg ccttcctgtt gagatggtgc ctgttcgccg
     3241 ctgggctgag gcgcaggctg gccgctgatc tcggcgtaga tgcgggcgta attgtcgggt
     3301 gtaggtggcg tcttgtgcgc ggccagcttg atcagcgtct cgcgggcgag ttcggagggg
     3361 ttggcagctt gcttgctcat ggttctatct cgatagcatg gcgtaggtcc gggcgaactc
     3421 tctggttatt ttttggcttc caccagatcg tgcgcgatgg cgtagtgtac ggcttcggca
     3481 ttgttgttga aacccatttt ttccagcatg cgggcgcggt agacgctcac ggtcttgggg
     3541 ctgactacca tgatctcgga gatgtcggtc agggattttc cggaggccat caggcacagg
     3601 gtctgatatt cgcggttaga caggctctga tgcaactgtt cctgcgattc accgatcagg
     3661 ttgctcagca gttgttcggc cagcgccggg ctgatgtatt tttttccgct gatcacctga
     3721 tggatggcgc cgaccagttt ttccggcgcg ctctgcttgt tgatgtaacc catcgcgcca
     3781 gccttgaggg cgcgtacgcc gtactggtct tccggataca tgctcagcac gatgattttg
     3841 atctcgggct gttcggattt gagctgtttg agcacgtcga tgccatgctt gtccgccagc
     3901 gaaatgtcga gcagcaccag atcgaagtgc tgttcgcgca ccatgttgat ggcctgcatg
     3961 ccggtttccg cttcgccctt gatgtggaac tcggggttct cttcaagcag catgatcaga
     4021 cccttgcgga ttaacgcgtg gtcgtctacc accagaatgt cgatcatatt gaggttccgc
     4081 cgaaaagtgg ctgttgtggg gtttcttccg gcaacggcac gaagaccgcg atttgcgtcc
     4141 ccagtcccag cgtgctggct attttgacgg tgccgcccag atgtgcgacg cgttcgtgga
     4201 tgccgcgcag cccgaaggag tgcggtttgt cgcgggcggc ttcgctgaag cctacgccgt
     4261 cgtcggagat gatcagctcg aggtggtctg cgcccttgac gacttcgatc tgtacctgcg
     4321 tggcctgcgc atgcttcagg atgttgttca gggtttcctg acagacgcgg aacagcgtga
     4381 tggtataggc ctcgggcagt tcgatgcttt cctgcgactt gatgatggtg cagggaatgc
     4441 cgctgcgttt gctgaattcc tgcgcctcga tctcgatcgc ggcgatgatg ccgaagcagt
     4501 ccagaaagcc gggacgcaga cggtgtgcca gatggctggc cgaggtgatc gcgcggttga
     4561 ccagttcttc tatggtcttg gccttgtcgg tcagcgtggg attgtccttg ggcaggcgct
     4621 ggatcagcca ggacagatcc atgcgcatcg cggtgagcag gctgccgatt tcgtcatgaa
     4681 cctcgcgcgc gatacgcagg cgctcctgtt ccttggcatc ctgaatgtgc gaggaaagct
     4741 ccagcaattg ctgctgtacg cgtttaacct cgagttcgcc cagcttgctc tgggtggtgt
     4801 tgagcacgat gccttcccag cgcggggcgc caccctgagt gtcttgcggc gtcgcgccca
     4861 gactgatcca tttggtctca ccgtccggca gcgtgatgcg gccttcccag ttccagaagc
     4921 agcgggtctg agccgaagtc ctcatgctct ccagcaacgc gggcgcatct tcggcatgga
     4981 taaggtcaag gagtcgcgtc gtgtcggcct gtaactcgct ggcagcgatg cccagcaacg
     5041 gctggcagcc ctcgctgaca taaggaaaag tgaactgtcc gtcttcctgc agctcgatct
     5101 ggcatgccat gccgggcagg ctggcgatga agccctggag tttgtccggg gatggactgg
     5161 tcggcggatt gggggcggct ttttgcatcg gggcagtcag gatgaatggt ctgttcaata
     5221 tcgattaagg gagaggttca agtcgcgctg cgccccggcg ggtatgacgc tctgggtatt
     5281 attgccgcgc ctctgacggg aaaggttact acaccctggc tgccggcagc aacattagtt
     5341 gccttggccg acaaaaatct ttacatggcg ggcagattgc tggagtattt gcgcggtttc
     5401 agaaaatcaa caacaagggt ggattatggc ggacatcaag gtaggcacaa gatttggcga
     5461 gttgagcgta gacccttttc aggttatcac tttccccaga gggataccgg ggtttgaaaa
     5521 tagcacgaag tggacattgt ttcatgagct tgacgagcaa gggggctggg cgagcggcgt
     5581 ggtggtctat cttcagtcgc tggatgatgg ggacatcgca ttgccgttga cggacccgac
     5641 cctgttcggc ttcaacttcg atctggtgtt gtctgacagc gagatcgccg aactccagct
     5701 cgaagatccg aacgatgtgc tggtgctgac gacgctgtcg ctcaaagacc ctgccgcgag
     5761 caagactcaa cgtccgtcga tggcagatat gtatgccaac atctctgcac ctattctcat
     5821 caataccaag tctcgcatcg gcatgcagaa ggcattgatc gcccgcgagt ccaaggtgga
     5881 ttttcacccc gcagcctgat ttattcatga ttcggatagg cgctgcgcag tgacgggcag
     5941 cgtctggtca catcggtttg cccgaggagt tcgatgatag tcccggaaga agtgttgcgg
     6001 cacgcgacca cgttttatga agccggtcgt ctggacgagg cagaagagtt gtgtctgggc
     6061 gtcctgctga cagagcccga gcatgcggcc gcgaatcatt atcttggttt gatcgcgttg
     6121 caacgagggc tggcggagcg tgccttgccg tatctgcaga tggcctggga ggtcgatcct
     6181 tcagcggggg cgcattggct gacgctggct gagtgcctgt tagcgctggg gcgcttcgaa
     6241 gacgcactga atctggtcaa ggaggcaagc aagcgcgggc tggatgcgcc gcaggtgcag
     6301 cagcttaggc tgctcgccaa gcgcgggctg gatcgcacgg cgccatccgc tgcggtggtc
     6361 gacgagatgt tggcgctgtt tcgggccgga cgcaatgcgg agctggagtc gcgggtgtca
     6421 ccgttgctcg aacagtatcc cggctgggtc tttggctggc gtgcgctggg cgcagccatg
     6481 gggatgctgg ggcgggatgc cgagccggtg ttgcggcgcg ccatggcgct tgcaccgcag
     6541 gacgccagta ctcacaacaa tctgggggtg gtattgcgtg agcgggggcg acaggcagag
     6601 agtctgatct gtttgcgcaa ggccgtgcag ctgagtccga aatacgccga ggcgcattac
     6661 aacctgggaa ccgtgctgca ggatatgagc cggtacgacg aggcgatagt ctgtttccgc
     6721 caggcgataa ggatcaagcc ggattatttc gaggctcagg ctgagttgag taattccctg
     6781 tctgctctgg ggcgcttcgc cgaggcgcta aaaatactgg aacgattgcc gaggtccagg
     6841 ccggatcatc caaaggcgct cagcaacctg ttgtttaacc tgacccaccg tggcgacatt
     6901 gacgcagaca cgctgtttgc cgagcactgc cgcttcgggg cgcagttcga cgcgccattg
     6961 caggcattcg ccaatgcgcg agaccacgag cgccgtctgc gggtcggcat cgtgtcgggc
     7021 gatctgcatc tccacgcggt cgcaaatttc atcgagccgg tattgctgca tctgagacat
     7081 agcgcgcaat tgtcgctgca tgcctattac aaccatgtgc aggaagacga ggtcacgcag
     7141 cgcttgaagg gatactttgc gcagtgggat tcggtgttcg gtctgacgga cgaggaactg
     7201 gcgcagaaga tacgtgcgga cggcatcgat atattgatcg atctctccgg ccacacggct
     7261 cgcaaccggc tgccgttgtt cgcgcgcaag cccgcaccga ttcaggcgag ct
//
LOCUS       DASUIV010000071        21048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_853420, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000071 DASUIV010000000
VERSION     DASUIV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 21048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21048
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853420"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VFW59_02645"
     CDS             <1..1043
                     /locus_tag="VFW59_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5363143.1"
                     /translation="RIALCVLLAGLAGCSAVSSTYKDAKHTVGDWFGSGADNTLTAEA
                     SKKPSGNATQLKYAATLRVSKYVDQRKVGNPRWLGVLTSRVKGISGRELIMDQDVATI
                     ATAAIKRQFDGEGFQVLEGSAADKALFEVSGVIKEMTLNVKERDDINIVIETTLKEVS
                     TGKVVWSGMVVEKSDRFAGVAGNDKEAVVAYMNWGLRTVNKKTVEAITTALMATRPEL
                     FNLTPGSKPIPGVTVFVAPTAASVAPAAAPVVNPYGMPGYGVPANAPVPASSYAPHAS
                     ATAGLLLVNTDPQRAKVYLDGVYYGLSPLRLEMEPGVHAISVKLEGYKMVTEKVSVRK
                     GDNTELELHLER"
     gene            complement(1118..1573)
                     /gene="dut"
                     /locus_tag="VFW59_02650"
     CDS             complement(1118..1573)
                     /gene="dut"
                     /locus_tag="VFW59_02650"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX5363144.1"
                     /translation="MKHLSVDVKILDQRLRENMPAYATGGSAGIDLRACIDQNIVIQP
                     KQCELIPSGIAIHLDNPGYAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGQVFVSLWN
                     RSQLPFTLTPMERMAQLVIVPVMQAKFNIVEEFPLSERGSGGFGSTGKH"
     gene            complement(1570..2760)
                     /gene="coaBC"
                     /locus_tag="VFW59_02655"
     CDS             complement(1570..2760)
                     /gene="coaBC"
                     /locus_tag="VFW59_02655"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5363145.1"
                     /translation="MEQASAKRILLGITGGIAAYKAAELVRLLKKQGFEVQVAMTGAA
                     TQFITPTTMQALSGLPVLLNQWQDESGMTHIHASRTADAIVIAPATADFIAKLAHGLA
                     DDLLSTLCLARDCPLIVAPAMNKQMWSNPATQRNVQQLVSDGIALLGPASGSQACGEE
                     GMGRMLEAAELAEEIIASFQPKLLEGVKLLMTAGPTYEAIDAVRGITNRSSGKMGYAI
                     ARAAREMGAQVTLVSGPVTLTPPIGIERIGVNSAQEMYDAVNRRAGEADIFIGVAAVA
                     DYRIAHPAEQKIKKSSAPMTLELIANPDILADVARLPNPPFCVGFAAESERLAEYAEH
                     KRRAKKLPLLVGNLAQQAIGSDDNELMLFDDEGCHPLPRSDKLTLARLLMQHIAKRYK
                     GANP"
     gene            2968..3642
                     /gene="radC"
                     /locus_tag="VFW59_02660"
     CDS             2968..3642
                     /gene="radC"
                     /locus_tag="VFW59_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX5363146.1"
                     /translation="MSITDWPPGERPREKLLQRGASSLSDAELLAIFLRTGVTGQSAV
                     DLARELLQRFGCLTRLFAATEDEFCAIHGMGQAKYVQLQAVLEMSRRALQEELQRGDA
                     LSSPRAVRDYLQLLLGARQQEVFLVLFLDTQHRVIAFEELFHGTLGQTSVYPREVVKR
                     ALSHNAAAVIFAHNHPSGVAEPSQSDRLLTDALTQALGLVDVRVLDHFVVAVGQTLSF
                     AERGLI"
     gene            3743..5266
                     /locus_tag="VFW59_02665"
     CDS             3743..5266
                     /locus_tag="VFW59_02665"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5363147.1"
                     /translation="MRFGRAAGLVLLMLASGGVDAQQVGSKTKKLHPVAKKTAVSEAA
                     TLRAQREAEARAKTELAAQQRAEAEVRKQAEIEAREKPLRDADELIKSGKPGDAYALL
                     EPLEFERSGEVRFDYLIGIAALDSGKPDKATLAFERVLAVDPNFAGARLDMARAYYQL
                     GDLARAKVEFETVLKAEPPEAARATIQKYLAAIDTRLHALDTRVTAYVEGTIGHDTNV
                     NNATAQTQIAVPALGNMVFVLNPSNQKTADSYLGLNGGLNVSHPVNETVSLYAGADVR
                     QRSNSVLTRFDSLSLDGRAGGVFKLGEQDSLRAGILGGQYNLNGQHNRDSLGVNGEWN
                     HVFSPANQMSVFLQQMAYRFVDAAMKPEDFDQSVLGAGWRHVLHDGKSAIFSSLFYGD
                     ERDVAPVSPSNLAGGRTDGGKQFIGLRVGGQAALSETIDVFANAGGIDGNYARTNVLF
                     QVKRHDVLTDMTIGSNWHWDKLWTLRPQLSWSRNQSNISLYGYDRIDASVTVRRDFN"
     gene            5300..6898
                     /locus_tag="VFW59_02670"
     CDS             5300..6898
                     /locus_tag="VFW59_02670"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX5363148.1"
                     /translation="MNKQRWSLMLPLILTGWCVVTDVWSAQAGRVQFVHGEVRVSDQA
                     GRVQPLQKGDAINEGDTLISASNASAQIKMMDGGFVAVRPDTRLKFDSFKFSGKAGEP
                     ESSFFSLFKGGFRAITGLIGRLNKQDYRITTPSATIGIRGTDHETVVVVPDSPLVLAG
                     QAASGTYNKVNAGETSLTTDKGSINVLPNQMGFAGGQNQMPKIQPINLNLFTVAPSPA
                     PGAAMEGGDADAARESAVVDNTAVAAAGDATTVAAAGSATVLPASVAPPPVMVTLTQN
                     LLPPFPTQLAGGLGFFYQDTAGGVWTTGGGGVAQVTPSASGGLGSFATSATFTSCIAN
                     CNYSIPFQSGSLGTASLLDQGSNILAGNLHWGRWFGQGATVVGLPAGDVLFNGNLTYI
                     GGDIPTMPISGAATYLPVGGTSPVDSAGNAGKFIGANVSVNFTNLSIAVSNMQLAFGG
                     NTYTMAGAGTFLSSGVIPSVPLAGSCAGTCTVGTAMNGDYAGSFTGVNAAGLALVYHV
                     ASSGLATNAVPVFEIMGSQAFIKQ"
     gene            complement(6973..8343)
                     /gene="purB"
                     /locus_tag="VFW59_02675"
     CDS             complement(6973..8343)
                     /gene="purB"
                     /locus_tag="VFW59_02675"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5363149.1"
                     /translation="MTLSALTALSPLDGRYAGKVDALRGYFSEFGLIRFRVLIEIEWL
                     KALSAQADIPEIAQFSAATIAQLDALNANFSEADATAIKTIEKTTNHDVKAVEYWLRE
                     KLGANPETAGVLEFIHFACTSEDINNLSHALMLKGAREAAMLPALQGVIARLKDLAHQ
                     LADVPMLCRTHGQTATPSTLGKEMANVVYRLQRAEQRLADVEILGKINGAVGNYNAHL
                     SAYPEVDWEAFAKRFVEARGITFNPYTIQIEPHDYMAELYDAYARINTILVDLDRDIW
                     GYISLGFFKQKVIKGEVGSSTMPHKVNPIDFENSEGNLGMANALLRHLSEKLPISRWQ
                     RDLTDSTVLRNMGVALGYTLLGYESLLKGLNKLEANPQRVADDLNNSWEVLAEPIQTV
                     MRRYNIENAYDKLKDLTRGKGGINRESLQAFIQTLDIPAAEKQRLLQLSPDTYIGKAA
                     ELAKRI"
     gene            complement(8510..10039)
                     /locus_tag="VFW59_02680"
     CDS             complement(8510..10039)
                     /locus_tag="VFW59_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEX5363150.1"
                     /translation="MQTATDEKFEAFIWDNKFNTNLDVVDSQHHKLVELINQLGSISA
                     HQTSTAALSAILTELANYTKYHFDTEEKMMADLGVDPVHQNMHIKAHQHFTAQVQVAA
                     QILLSRSDVSGQIVAPLLRYLTNWLVQHILGSDKRMAQEILALQAGSSHEEAIRKATN
                     FMSEAANVLLEALNEMYGKLGDKTLEVIQKNQELLAEREALHVLNEQLEQRVVQRTAD
                     VVQANQQLLANNLELKQLNEKLASTQTQLLQSEKMASIGQLAAGVAHEINNPVGFVNS
                     NLGTLTKYIASLFKVIDAYEAEEAKSNAKSYPEVAQLKKDVDYPFLAEDIPSLLSESR
                     DGLARVTRIVQDLKDFSHIDESVWQYANLEQGMDSTLNVVSNEIKYKAEVVREYAGLP
                     EIECMPSQINQVFLNLLLNATQAIDVKGVITVRTGTLGEEVWVEVADTGKGIPPENLN
                     RIFDPFFTTKPIGKGTGLGLSLSYGIVQKHNGRIEVESEPGKGTTFRVWLPVRQQTGN
                     R"
     gene            complement(10174..11442)
                     /locus_tag="VFW59_02685"
     CDS             complement(10174..11442)
                     /locus_tag="VFW59_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5363151.1"
                     /translation="MTMETEIAPPATGRILLVDDEASILAALRRLLRTRGYEIFSANS
                     GAEGLLILERESIDVVISDMRMPEMTGAQFLEHVFSRWPDVKRILLTGYSDVESTVAA
                     VNRGKIWRYIPKPWDDEDLLLTVQQALAHRQLMDENRHLVTELKALNTTLEKRVDERT
                     AQLQQALKTMRQSFVNTVHVFSNMMDLRGGALAGHSRRVAEHARKVAKQLGMEETAAQ
                     DTFLAALLHDIGKLGLPDAAMEQPFNALGSELRAEVMKHPAKGEMLLMPIPQLAGAAA
                     LIRDHHEQFDGHGYPSGKSGLDIPLGARILAAVNDYDALQLGLLATRKLTPSEALHYL
                     VENSGKRYDPDVVDAFSAVLTETHPDTFAEVTLRPASLKLGLRLSRDLLHREGYLLLP
                     KDHIITAADINQLMRLEATEFHPLTLYVVK"
     gene            complement(11571..12623)
                     /locus_tag="VFW59_02690"
     CDS             complement(11571..12623)
                     /locus_tag="VFW59_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5363152.1"
                     /translation="MPVNLSPEIVRLLLVLTVTLVAHFIAKRVLRHAGQAALRTSNIW
                     DDSLVEAARRPLPMLIWLIGGSFVLHLIHRQTGEQLLDYLAPTRDIGFVICIAWFLFK
                     LIRELANNVVAARAQSGEPVDRTTVDGLSKVSRIIVLVLSALAIVQTLGFSISGALAF
                     GGVGGIAVGFAAKDLLANFFGGLMIHLDRPFNIGETIRSPDKQIEGKIEHIGWRQTSI
                     RASNMALIYVPNSLFTSIVVENPSRMSHRRIREIIGLRYDDMDKVSRIAEDIRAMLRD
                     HAEIDAEAGMIVALDQLADSSLNLLVQAFSKATEGERFHAAKQEILLNIAHIVARHGA
                     EIAFPTRTLHLHQTPA"
     gene            complement(12639..13727)
                     /gene="mnmA"
                     /locus_tag="VFW59_02695"
     CDS             complement(12639..13727)
                     /gene="mnmA"
                     /locus_tag="VFW59_02695"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5363153.1"
                     /translation="MKSNPQSRRVVVGMSGGVDSSVTALLLKQQGYDVVGLFMKNWED
                     DDNSEYCSSRQDLLDAVSVADTIGIPIEAVNFAKEYKDRVFSYFLREYESGRTPNPDI
                     LCNSEIKFKAFLDHAIQLGADSIATGHYARVREVDGLFQLLKAADASKDQSYFLHRLN
                     QAQLSKAMFPLGDMLKSEVRAIAEQHGPSNYAKKDSTGICFIGERPFREFLNRYLPAQ
                     PGEMRTPEGKVVGRHMGLSFYTIGQRQGLGIGGGKDSTGEPWFVAAKDMANNRLIVVQ
                     GHDHPALLNPHLEALDMHWIAGHAPDLTRAFAAKTRYRQTDAPCRIAIGNDNRCSFDF
                     DEAQWAVTPGQSVVAYDGDICLGGGIIE"
     gene            complement(13779..14225)
                     /locus_tag="VFW59_02700"
     CDS             complement(13779..14225)
                     /locus_tag="VFW59_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5363154.1"
                     /translation="MIWKPNVTVAAVIERDGRFLLVEEETEQGLRFNQPAGHWEPNET
                     LAQGAAREVLEESAWHFTPQHLIGIYRWHSDISNVTYLRFAFTGAITAHEPDRALDDG
                     IVRAAWMTIDEVRATQERHRSPLILRCIEDYLAGKRYPLDLLVHYD"
     gene            complement(14290..14376)
                     /locus_tag="VFW59_02705"
     tRNA            complement(14290..14376)
                     /locus_tag="VFW59_02705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14340..14342),aa:Leu,seq:caa)
     gene            14478..15503
                     /gene="queA"
                     /locus_tag="VFW59_02710"
     CDS             14478..15503
                     /gene="queA"
                     /locus_tag="VFW59_02710"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX5363155.1"
                     /translation="MRTEEFDFDLPERLIAQHPPERRGASRLLYAHDGQLEDCVFADL
                     LRLVGPNDLLVLNDTRVIKARLFGEKETGGKIEVLVERVLDEHEVWAQVRASKSPKAG
                     TRLLLADSLMVEVLGREGEFFHLRFLADEPVLDLLERHGRLPLPPYITHAAEAADDER
                     YQTVFAREAGAVAAPTAGLHFSDAMLQALRDKGVSIAHVTLHVGAGTFQPVRVEQIHE
                     HSMHSERYEIPQATVAAIAATRAAGGRVISVGTTSLRALESAAAAGELKAGSGETRIF
                     ITPGYRFRVVDVLLTNFHLPRSTLLMLVSAFGGMAQMRAAYRHAVAQEYRFFSYGDAM
                     LIERLRA"
     gene            15556..15975
                     /locus_tag="VFW59_02715"
     CDS             15556..15975
                     /locus_tag="VFW59_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5363156.1"
                     /translation="MEPYVLWFLLALGLFIAEMMTSTFYMMVLSIAMCVGGVAALMDM
                     SPAVQLGLGAMASIVGVVLLSRWKARQPAAPQDDQPDIGQPVRVLFWREDGTARVHYR
                     GADWDAEPESADMPQAGSCYIKALHGSILILSQHKPQ"
     gene            15992..16900
                     /locus_tag="VFW59_02720"
     CDS             15992..16900
                     /locus_tag="VFW59_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEX5363157.1"
                     /translation="MEIALFILIGAFIFIIKALKVVPQQNAWVVERLGRFHVTLTPGL
                     SIVVPFVDRVAYRHSLKEIPRDVPSQICITKDNTQLQVDGILYFQVTDPRLASYGTSD
                     YVFAITQLAQTTLRSVIGKMELDKTFEERDDINRAVVAALDEAASSWGVKVLRYEIKD
                     LTPPKEILHAMQAQITAEREKRALIAASEGRKQEQVNIATGEREAAILRSEGNKQAAI
                     NNAQGEAEALKAVADGAAQAIRMVAEAIEAPGGMNAVNLKVAEKYVEAFGNVAKEGNT
                     LILPANLADMGGMVAAAMSIVKAQKG"
     gene            16905..18008
                     /gene="tgt"
                     /locus_tag="VFW59_02725"
     CDS             16905..18008
                     /gene="tgt"
                     /locus_tag="VFW59_02725"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5363158.1"
                     /translation="MEFSLHKRDGLARRGTLQLAHGKVETPAFMPVGTYGTVKAMSPV
                     ELHDIDAHIVLGNTFHLWLRPGLEVIAAHGGLHQFMGWDGPILTDSGGFQVFSLGALR
                     KITEEGVKFQSPVNGDKCFLSPEESMRIQKVLNSDIVMIFDECTPYPASERDAADSMR
                     LSLRWAARSKAAHEGNPNALFGIVQGGMYENLRDESMGELVKIGFDGYAIGGLSVGEP
                     KEDMLRILKHTAPQLPQDKPRYLMGVGTPEDIVAAVTQGIDMFDCVMPTRNARNGMMF
                     TRHGDIKIKNAQYRLDTRPLDEDCQCYTCRNFSRSYLHHLFRLGEILGARLNTIHNLH
                     YYQDLMRDIRASIEAGEFDEFVAKFHRMRAGKA"
     gene            18069..18395
                     /gene="yajC"
                     /locus_tag="VFW59_02730"
     CDS             18069..18395
                     /gene="yajC"
                     /locus_tag="VFW59_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX5363159.1"
                     /translation="MFISNAYAETAAAGSQGGIMEFLPLIALLAVFYFLVLRPQSKRA
                     KEHKALVEALQRGDEVVTVGGQVGSVSKVFEEYVAVEVAENVEVTVQKSAIQNVLPKG
                     TIKSIK"
     gene            18468..20276
                     /gene="secD"
                     /locus_tag="VFW59_02735"
     CDS             18468..20276
                     /gene="secD"
                     /locus_tag="VFW59_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX5363160.1"
                     /translation="MNRYPLWKYLLILAVLLAGLIYTLPNFYGESPAVQVSPLRASLK
                     ADAALLERVTGILREAGLKPEVVALDGNSVKARFGGTDEQIKARDLLIAKLGEDYMAA
                     LNLLSRSPSWLARFASPMYLGLDLRGGVHFLLQVDMKAALDKALEAASSDIRSALREQ
                     NIAYSGVARDGNVMVVRFRDAEARGKGEAEIKQRFNDYDLSAKEEGSDFLLRATLKPE
                     AERRIQESAVQQNLLTLRNRVNELGVAEPVIQQQGLDRVVVQLPGVQDTARAKDILGR
                     TATLEVRMVDDEHQITPGAALPFGTELFKDREGNQLLVKKQVILTGERISDAQPGFDS
                     RNNEPAVHINLDNVGARKFKDATRENVGKRMAIILFEKGRGEVVTAPVIREEIGGGRV
                     QISGQMNTVEAQDTSLLLRAGALAAPMEIVEERTIGPSLGAENIQRGFDSTKIGFIMI
                     AVFMGVYYLMFGAISVLALGANLLLLVAMLSMLQATLTLPGMAGIALTLGMAIDANVL
                     INERIREELRNGVTPQAAIHAGYDRAFSTILDSNITTLIAGVTLFMFGSGPVKGFAVV
                     LCLGILTSMFSAMLVSRALVNLIYGRKARLNKVAIG"
     gene            20382..>21048
                     /gene="secF"
                     /locus_tag="VFW59_02740"
     CDS             20382..>21048
                     /gene="secF"
                     /locus_tag="VFW59_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5363161.1"
                     /translation="MELFKIKRDIPFMSYGKYTTTISLLTFIVAVFFLSTKGLNFGVD
                     FTGGTVMEVHYAQPADIPKVREQLAATGQPEALVQTFGTSQDVLIQIPLKQNLTGAKL
                     SEQVIAGLRAQDATVEMRRVEFVGPQVGRDLVENGALALLLVSLGIVGYLWLRFEWKF
                     GLSALIANLHDVVIILGFFAFFQWEFSLSVLAAVLAVLGYSVNESVVVFDRIRENFRS
                     MRKA"
BASE COUNT         3969 a         5943 c         6735 g         4401 t
ORIGIN      
        1 ggcgtatcgc gttgtgtgtg ttgctggccg ggcttgcggg ctgttcggcc gtgagttcta
       61 cctataagga tgccaagcat acggtgggtg actggtttgg tagcggtgcc gacaatacgc
      121 taactgcgga agcgagcaaa aagccatcgg ggaatgcgac tcagctcaaa tatgccgcga
      181 cactgcgagt cagcaagtat gtcgatcagc gcaaggtcgg caatccgcgt tggctggggg
      241 tgctcacttc ccgtgtgaaa ggtatcagcg gtcgcgagtt gatcatggat caggatgtcg
      301 cgaccatcgc aacggctgca atcaagcgtc aattcgatgg cgagggattc caggtgctgg
      361 agggcagtgc ggccgacaaa gctttgtttg aagtctccgg tgtcatcaag gaaatgacgt
      421 tgaacgtcaa agagcgcgat gacataaaca tcgtgatcga gacgacgctg aaagaggtgt
      481 ccaccggtaa ggtagtctgg tcgggcatgg tggtcgagaa gagcgatcgt ttcgcaggcg
      541 tggccgggaa cgataaggaa gcggtcgttg cctatatgaa ctgggggttg cgtaccgtca
      601 acaagaaaac cgtagaagca atcacgacgg cgttgatggc gacccgtccg gagttgttca
      661 atctgacgcc gggcagcaag cctattccgg gggtgacggt ctttgttgcg ccaacggctg
      721 cgtccgtggc gccagcagcg gctcctgtcg ttaatcctta cggcatgcct ggatacggag
      781 tgccagccaa tgcgcccgtt ccggcctcgt cctatgcgcc gcatgccagc gccacggcgg
      841 gtttgttgct ggtgaacacc gatccgcagc gcgccaaggt gtatctggat ggcgtgtatt
      901 acgggctgtc tccgctgcgt ctggaaatgg agccgggtgt gcatgcgatc agcgtcaagc
      961 tggaaggcta caagatggtc accgagaagg tctcggttcg caagggtgac aacaccgagc
     1021 tggagttgca tctcgaacgt tgatgatgca ttcgccacca taaaaaagcc cgcatcagcg
     1081 ggcttttttg catttgcagg gccgaacccc tgcaatatca gtgtttgccc gtgctgccga
     1141 atccgccgct gccgcgttcg ctcaacggga attcttcgac gatgttgaac ttggcctgca
     1201 tcaccggcac gatcaccagt tgtgccatgc gctccatcgg ggtcagcgtg aaaggcagct
     1261 ggctgcgatt ccatagcgag acgaagacct gcccctgata gtcggagtcg atcagtccga
     1321 ccagattgcc gagcacgatg ccatgtttgt ggcctagccc cgagcgcggc aggatcatcg
     1381 cggcatagcc gggattgtcc agatggatcg cgatgccgct ggggatcagt tcgcactgct
     1441 tgggctggat cacgatgttc tgatcgatgc aggcacgcag gtcgattccg gcggagcctc
     1501 ccgttgcgta ggcgggcatg ttttcacgca ggcgttgatc gagaatcttg acatctaccg
     1561 agaggtgttt catgggttcg ctcctttgta tcgttttgca atgtgttgca tcaatagccg
     1621 tgccagcgtc agcttgtcgc tgcgcggcaa cggatgacaa ccctcgtcgt cgaacagcat
     1681 caactcgttg tcgtcgctgc ctatcgcttg ttgcgcgagg ttgccgacta acagcggaag
     1741 tttcttggcg cggcgtttgt gctcggcgta ttcggcgagc ctctcgcttt cggcagcgaa
     1801 gcccacgcag aacggcggat tgggcaggcg ggcgacatcg gcgaggatgt ccgggttggc
     1861 gatcagctcc agcgtcatcg gcgcgctgga ttttttgatc ttctgttcgg cggggtgggc
     1921 gatgcggtaa tcagccaccg ccgcgacgcc gatgaagatg tccgcctcgc ctgcgcggcg
     1981 attcacggca tcgtacattt cctgtgcgct gttgacgccg attcgctcta taccgatggg
     2041 tggcgtcagt gtgaccgggc cggacaccag tgtcacctgc gcgcccatct cgcgggcggc
     2101 tcgcgcaatc gcatagccca tcttgccgga gctgcggttg gtgatgccgc gcaccgcgtc
     2161 gatcgcctcg taggtgggac cggcggtcat cagcagcttc acgccttcga gcagcttggg
     2221 ctggaaactg gcgatgattt cctcggccag ttccgccgcc tccagcatcc gccccatgcc
     2281 ctcttcgccg caggcctgcg atccgctggc cggtcccagc agcgcaatac cgtcggagac
     2341 aagctgttgc acattgcgct gcgtggccgg attgctccac atctgtttgt tcatcgccgg
     2401 tgcgacgatc agcgggcaat cgcgcgccag gcacagcgtg gagagcaggt cgtcagcgag
     2461 gccgtgggcg agcttggcga tgaaatccgc cgtagcaggc gcaatcacga tcgcgtccgc
     2521 cgtgcggctg gcatggatgt gagtcatgcc ggattcgtcc tgccattggt ttagcagcac
     2581 tggcaggccg gacagcgcct gcatcgtggt cggtgtaatg aactgtgtcg cggctcctgt
     2641 catcgccacc tgcacctcga agccctgctt cttcagcagg cgcaccagtt ccgccgcctt
     2701 gtaggccgcg atgccgccgg tgatgccgag caggatgcgt tttgccgatg cttgttccat
     2761 aatgctgcgc atcttacaag aaaatcacta cgcgctggct gttggcttca cgttcgttcc
     2821 ctcaatcaga caggaggcga agcatgagca tcaccgattg gccctctgga gaacgtccac
     2881 gggacaacac gtcgcgtagc ggcttgttgc ggcgtaggct aaccttcagg ttatgccgta
     2941 gccaaaagct atgcgggagg tgcaagcgtg agcatcactg actggcctcc cggagaacgt
     3001 ccacgggaga agcttctgca gcgcggtgca tcctcgcttt ccgatgccga actgctggcg
     3061 atctttctgc gcaccggtgt gaccggccag agcgcggtcg atctggcgcg cgagttgctc
     3121 cagcgcttcg gctgcctgac gcgcttgttc gccgcgaccg aggacgagtt ctgcgcgatc
     3181 cacggcatgg ggcaggcgaa atatgtgcag ctgcaggcgg tgctggagat gtcgcggcgc
     3241 gcgttgcagg aagagttgca gcgcggcgat gcattgagtt caccgcgtgc ggtgcgcgac
     3301 tatttgcaac tgttgctcgg agcgcggcag caggaggttt ttctggtgct gttcctcgat
     3361 acccagcatc gcgtgatcgc gttcgaggag ttattccacg gcacgttggg ccaaaccagc
     3421 gtctatccgc gcgaggtggt gaagcgcgcg ctgtcgcaca atgcggctgc cgtgatcttc
     3481 gcgcacaacc atccctccgg cgtcgccgaa cccagccagt ccgaccgatt gctgaccgat
     3541 gcgctcacgc aggctctggg gctggtggac gtgcgggtgc tggatcactt tgtggtggcg
     3601 gtcgggcaga ctctgtcgtt tgcggagcgc gggctgattt gacgataggg agcatcaaat
     3661 acggggggct atcggcatca cctgattggc aggtcgtctg gcggggcgta acatgagcaa
     3721 ataaattaca agggaggatg ttatgagatt cggacgtgct gcaggtctgg tgttattgat
     3781 gctggcatca ggtggggttg atgcacagca agtcggcagc aaaaccaaaa aactccatcc
     3841 cgttgcaaaa aaaacagccg tttcggaagc ggctacgctg cgggcacagc gcgaggcgga
     3901 ggcgcgagcc aagactgaac tcgccgcgca gcaacgagcc gaggcggaag tgcggaagca
     3961 ggctgaaatc gaggcgcgcg agaagccgct gcgcgatgcc gatgaactca tcaagagtgg
     4021 caagcccggc gacgcctatg ccttgctgga acctctggag ttcgagcgtt ccggcgaggt
     4081 gcgtttcgac tatctgatcg gtatcgcggc gctggacagc ggcaagccgg acaaggcgac
     4141 gctcgcattc gagcgggtgt tggcggtcga tcccaacttc gccggcgcgc gcttggacat
     4201 ggcgcgcgct tattatcagc tgggcgatct ggctcgcgcc aaggtcgagt tcgaaacggt
     4261 gctaaaggcc gagccgcctg aggccgcgcg cgcaaccatt caaaaatatc tggcggccat
     4321 cgatacccgg ttgcacgcac tggatacgcg cgtgaccgct tatgtcgaag gcacgatagg
     4381 gcatgatacg aatgtcaaca acgccaccgc acagacgcag atcgccgtgc cagcgctggg
     4441 caacatggtc tttgtcctga atccgagcaa ccagaagacg gcagattcct atctcggttt
     4501 gaatggcggc ctgaatgtgt cgcatcccgt gaatgaaacg gtatcgctgt atgcaggggc
     4561 ggatgttcgt cagcgcagca attcggtact gacccgattc gattcgctca gcctcgatgg
     4621 acgtgccggc ggggtgttca agctgggcga acaggatagt ctgcgtgcgg gcatattggg
     4681 cgggcaatat aacctgaacg gccagcataa ccgcgacagt ctgggcgtga atggcgagtg
     4741 gaaccatgtg ttcagtccgg cgaaccagat gtcggtgttc cttcagcaaa tggcttaccg
     4801 ctttgtcgat gcggcgatga agccggaaga tttcgaccag agtgtgttgg gtgccggttg
     4861 gcggcatgtg ttgcacgatg gcaagtcggc gattttcagc agcctgtttt acggggatga
     4921 gcgcgatgtc gctccggtct cgccgagcaa tcttgccggt gggcggaccg acggcggcaa
     4981 acaattcatc gggctgcggg tgggtgggca ggccgcgctg tccgagacca tcgatgtctt
     5041 cgccaatgcg ggcgggatag atggcaacta cgccagaacc aatgtcctgt ttcaggtgaa
     5101 gcggcacgat gtgttgacgg atatgacgat cggttcaaac tggcactggg acaagctctg
     5161 gacgctgcgt ccgcagcttt cctggtctcg caatcagtcg aacatttcgc tctatgggta
     5221 tgaccggatt gacgcttcgg tgacggtgcg gcgcgatttt aattgagccg tggcgcggat
     5281 cgactttgag gagaagaata tgaacaagca aagatggtct ctgatgctgc ccttgattct
     5341 gacgggatgg tgtgtcgtaa cggatgtctg gtcggcgcag gcggggcggg tgcagttcgt
     5401 tcatggcgag gtgagggtaa gtgaccaggc tgggcgggta cagccgctgc agaaaggcga
     5461 tgcgatcaac gagggcgaca ccttgatctc ggcatccaac gcctcggcgc aaatcaagat
     5521 gatggacgga ggattcgtcg cagtgcgtcc cgacacccgg ctcaagttcg acagtttcaa
     5581 gttcagcggt aaggcggggg agccggaaag cagctttttc tcgctgttca aggggggctt
     5641 ccgcgcgatc accggcctga tcggccggct gaataaacag gactatcgca tcaccacgcc
     5701 gagtgccacc atcggcattc gcggcaccga ccatgagacc gtggtggtgg tgccggatag
     5761 tccgctggtg ctggcggggc aggctgcgtc gggcacttat aacaaggtga acgccggcga
     5821 gaccagcctc accacggaca agggttcgat caatgtgttg ccgaaccaga tggggtttgc
     5881 cggcggtcag aaccagatgc cgaagataca gccgatcaac ctgaatctgt ttaccgtcgc
     5941 gccgtcacct gctccaggag ccgccatgga gggcggtgac gcggacgcgg cccgggaatc
     6001 ggcggtcgtg gacaatacgg cggtcgcggc ggcaggggat gcgactaccg ttgcggcagc
     6061 gggtagtgcg accgtgttgc ccgccagtgt ggcgcctcct ccagtgatgg taaccctcac
     6121 gcaaaactta ttgccgccgt tccccaccca gcttgcgggc ggcctcggtt ttttctatca
     6181 agacactgcg ggcggtgtct ggaccacagg cggcggcggc gtggcccagg tgacaccgag
     6241 cgcttccggg gggctgggca gttttgcgac cagcgccaca ttcacttcct gtatcgccaa
     6301 ctgcaactat agtatcccct tccagagcgg cagtctgggc acggcgtcgc tgctggatca
     6361 gggctcgaac atactggctg gcaatctgca ttgggggcgc tggttcggtc agggcgcgac
     6421 cgttgtaggc ttgcctgccg gagacgtgct ctttaacggt aatttgactt atatcggggg
     6481 tgacattcct accatgccaa tcagcggggc ggcgacctat ctgcctgtcg gcggtacctc
     6541 gccggtggat tcggcaggca atgccgggaa attcatcggg gcgaacgtca gcgtgaactt
     6601 cacgaatctg agcatcgcgg tcagcaatat gcagctagcg ttcggcggga acacctatac
     6661 catggccggg gcgggaacgt tcctgtccag cggggtcatt cccagcgttc cgttggcggg
     6721 aagctgtgcc ggaacttgta cggttggaac cgcaatgaat ggagattatg cggggtcttt
     6781 caccggggtg aatgccgccg gtttggcctt ggtctaccat gtggctagca gcggcctggc
     6841 gaccaatgct gttccggtat tcgaaatcat ggggtcgcaa gctttcatca agcaatgacg
     6901 ccagagtcga tccgctaacg aacaagcccg cgaaagcggg cttgttcgtt tcaggcgttt
     6961 acaggttgag gcctagatgc gtttggccag ttccgcagcc ttgccgatgt aggtgtcggg
     7021 cgacagttgc agcaggcgct gcttctcggc ggcggggatg tccagcgtct ggatgaaggc
     7081 ttgcagcgac tcgcggttga tgccgccctt gccgcgcgtc aggtctttca gtttgtcgta
     7141 ggcgttctcg atgttgtaac ggcgcatcac ggtctggatc ggttcggcca gcacttccca
     7201 gctgttgttc aggtcgtccg ccacgcgctg cgggttggct tccagcttgt tcagcccctt
     7261 gagcagcgac tcgtagccga gcagcgtgta gcccagcgcc acgcccatgt tgcgcagcac
     7321 ggtggagtcg gtcaggtcgc gctgccagcg cgagatcggc agtttctctg acaggtggcg
     7381 cagcagcgcg ttggccatgc cgaggttgcc ctccgagttc tcgaagtcga tcggattgac
     7441 cttgtgcggc atggtcgagg agccgacttc gcccttgatg actttctgct tgaagaagcc
     7501 cagcgagata tagccccaga tgtcgcggtc gaggtcgacc agaatggtgt tgatgcgagc
     7561 gtaggcgtcg tacagttccg ccatgtaatc gtgcggctcg atctggatgg tgtagggatt
     7621 gaaggtgatg ccgcgcgcct cgacgaagcg cttggcgaag gcttcccagt ccacttccgg
     7681 gtaggccgac agatgcgcgt tgtagttgcc gaccgcgccg ttgatcttgc cgagaatctc
     7741 cacatcggcg agacgctgtt cggcgcgctg caggcggtac accacgttcg ccatttcctt
     7801 gcccagcgtg ctgggggtgg cggtctggcc gtgggtgcgg cacagcatcg gcacgtcggc
     7861 gagctggtgc gcaagatcct tcagtcttgc gatcacgcct tgcagtgccg gcagcatcgc
     7921 cgcctcgcgc gcacccttga gcatcagcgc atgggacagg ttgttgatgt cttccgaggt
     7981 gcaggcgaaa tggatgaact ccagcacacc agccgtttcc gggttggcgc ccagtttctc
     8041 gcgcaaccag tattccacgg ccttcacgtc gtgattggtg gtcttctcga tggtcttgat
     8101 cgcggtggca tcggcctcgc tgaagttcgc gttgagcgca tccagctgcg cgatggtcgc
     8161 ggcggagaat tgtgcgatct cgggaatatc ggcctgcgcg ctgagcgcct tgagccattc
     8221 gatctcgatc agcacgcgga agcggatcag gccgaattcg ctgaaatagc cgcgcagggc
     8281 atcgaccttg ccggcgtagc ggccatcgag aggggaaagg gcggtgaggg cagaaagagt
     8341 catggcaaat acgaatgaat tttggaatgc cgctatttta cccttgccga agcttcatgg
     8401 cagcgctttc aagcttgttt gtgatgcgaa tacctcagag tgccttccgg catcgcagaa
     8461 cgccatgcaa tcgccagaaa atgagtctgc tgcgagcccc ggcgtgggct tatcggttgc
     8521 cagtttgctg ccgtaccggc agccataccc tgaaggtggt gcccttgcca ggttcgcttt
     8581 cgacctcgat gcgcccgttg tgtttctgga cgataccgta cgatagcgac aagcctaaac
     8641 cggtgccttt gccgatgggc ttggtcgtga agaaggggtc gaagatacgg ttgagatttt
     8701 cgggggggat gcctttcccc gtgtcggcca cttccaccca gacttcttct ccgagggttc
     8761 ccgtgcgaac cgtgatgacc cccttcacgt cgatggcctg agtcgcattc agcagcagat
     8821 tcaaaaatac ctgattgatc tgtgacggca tgcattcaat ctcgggcagc ccggcatatt
     8881 ccctgaccac ctccgccttg tatttgatct cgttcgagac gacgttgagc gtgctgtcca
     8941 tgccctgctc gaggttggca tattgccaga cagattcgtc tatatgagaa aagtctttca
     9001 agtcctgaac gatgcgcgtg acccgcgcca gtccatcccg cgactcactc aacaggctgg
     9061 ggatatcctc cgcgagaaag ggatagtcca cgtccttttt gagctgcgct acttccggat
     9121 agctcttggc gttgctcttg gcttcttctg cttcatatgc atcaatcacc ttgaacaggc
     9181 ttgcaatgta tttagtcagc gtccccagat tggaattgac gaagcccacg gggttgttga
     9241 tctcgtgggc gaccccggcc gccagttgac cgatagacgc catcttttcc gattgcagca
     9301 actgggtctg ggtggaggcg agcttttcgt tgagttgttt cagctcaagg ttgttggcaa
     9361 gcagctgctg gttggcttgc acgacatctg cggtgcgctg caccacccgt tgctccagct
     9421 gttcattcaa tacatgtaac gcctcgcgtt ccgccagcag ctcctgattc ttctgaatca
     9481 cctccagggt cttgtcgcca agcttgccat acatttcgtt caaggcttcg agcagcacgt
     9541 tcgccgcttc gctcatgaaa ttagtcgctt tgcgaatggc ctcctcgtgg ctggaacccg
     9601 cctgcaatgc cagtatctcc tgagccatgc gtttgtccga gcccaggata tgctgcacca
     9661 gccagttggt cagatacctc aataacggtg ccacgatctg gccggaaaca tcgctacgac
     9721 tcaacagaat ctgcgcggcg acttgcacct gagcggtgaa gtgctgatgc gccttgatat
     9781 gcatgttctg atgaacggga tctaccccga gatccgccat cattttttcc tcggtatcga
     9841 aatggtattt cgtgtagttc gccagttcgg tcaggatggc gctgagcgct gcggtgctgg
     9901 tctggtgtgc gctgatggaa cccagctggt tgatgagttc caccagtttg tggtgctggc
     9961 tatccaccac atcaaggttg gtgttgaact tgttgtccca gatgaaagcc tcgaattttt
    10021 cgtcagtcgc cgtttgcata tcgcttccct tcaatacctg aaaagtatct cttcagacag
    10081 ttcatctgct gcttatcccc gctgcatctt ccagtccacg tgcatgcacc tgggaaaggg
    10141 catcgaagcg accccattaa aattccacgc gcttcacttt acaacgtaaa gggtgagggg
    10201 atgaaactcc gtcgcttcaa ggcgcatcaa ctgattaata tcggcggccg taatgatgtg
    10261 atctttgggg agcagcagat aaccttcccg gtgcagtaaa tcccgcgata acctgaggcc
    10321 cagcttcaat gaggccggac ggagggtgac ttccgcgaag gtgtccggat gcgtttcggt
    10381 gagaacggcg ctaaaagcat caaccacatc gggatcgtaa cgcttcccgc tattttccac
    10441 gaggtaatgc agcgcctccg acggcgtcag cttgcgcgtc gcgagcaaac ccagttggag
    10501 cgcgtcatag tcgttgaccg ccgccagaat acgcgccccc agagggatat caaggccgct
    10561 ttttccgctg ggatagccat ggccgtcgaa ctgctcgtga tgatcgcgaa tcagggctgc
    10621 tgcgcccgcc agttgtggga tcggcatcag cagcatctca cccttggcag gatgcttcat
    10681 cacttctgca cgcagctcgg atcccagcgc attgaaaggc tgctccatcg cggcatcggg
    10741 caatccgagt ttgccgatgt catgcaacaa cgcggcaaga aaggtgtcct gcgccgccgt
    10801 ctcttccatg cccagctgtt ttgccacctt gcgcgcatgc tctgcaaccc ggcgcgaatg
    10861 gcccgccagt gcgccgcctc gcagatccat catgttcgag aaaacatgca cggtattgac
    10921 gaagctttgt cgcatcgtct tgagcgcctg ttgcaactgt gcggtacgct catccacccg
    10981 cttttcaagc gtcgtgttga gagccttcag ctccgtcacc agatgccggt tctcatccat
    11041 cagttggcgg tgagccagtg cctgctggac cgtcagcagc agatcctcgt catcccatgg
    11101 cttggggata tagcgccaga tctttccccg gttaactgcg gcgaccgtgg attcgacatc
    11161 ggaatagccg gtgagcagga tgcgtttgac atccggccag cgtgagaaaa catgctcgag
    11221 gaactgtgcg cccgtcatct ccggcatgcg catatcgctg atgacgacat cgatcgattc
    11281 gcgttcgagt atcagcaatc cttccgcacc tgaatttgcg ctgaagatct catatccccg
    11341 cgtccgcaag agccgacgca acgcagccag aatggaagcc tcgtcatcga ccagcaatat
    11401 ccgtcccgta gcggggggcg caatttcagt ttccatcgtc atggcgagat tgctcctgtc
    11461 gggcgtaagg ttgatcccgt cccgtatttt agacgcatcg gccgcagatc atggcgccat
    11521 cttgaaaaac agcagaagcg atcccgcttc gtcaaaattg acgtgggtcg tcaggccggt
    11581 gtctggtgca gatgcaaggt gcgtgtcggg aaggcgatct cggcgccatg tcgggcgacg
    11641 atatgggcga tgttcagcaa tatctcctgc ttggccgcat ggaagcgctc gccctcggtg
    11701 gccttgctga atgcctgaac cagcaggttg agcgatgaat cggccaattg atccagcgcg
    11761 acgatcatcc ccgcttcggc atcgatttcc gcatggtcgc gcagcatggc tctgatgtct
    11821 tcggcgatgc ggctcacctt gtccatatcg tcatagcgca ggccgatgat ttcgcggata
    11881 cggcggtgcg acatgcgcga cggattctcc acgacgatgg aggtgaacag cgagttggga
    11941 acgtagatca gcgccatgtt gctcgcgcgg atgctggttt gccgccagcc gatgtgttcg
    12001 atcttgcctt cgatctgctt gtccggcgaa cgtatcgttt cgccgatatt gaaggggcgg
    12061 tccagatgga tcatcaggcc gccgaagaag ttcgccagca agtccttggc ggcaaagccg
    12121 acggcgatgc cgcccacgcc gccgaaagcc agcgcgccgg agatgctgaa gcccagcgtc
    12181 tgcacgatgg ccaatgccga aagcaccagt acgatgatgc gggatacctt gctcaggccg
    12241 tccacggtgg tgcggtctac tggctcacct gattgcgcac gggcggcgac gacattgttg
    12301 gccagttcgc gtatcagttt gaacaggaac caggcgatgc agatcacgaa tccgatatcg
    12361 cgggtcgggg cgagatagtc gagcaattgc tcgccggtct ggcggtggat caggtgcagc
    12421 acaaacgagc cgccgatcag ccagatcagc atgggtagcg gtctgcgcgc cgcctcgacc
    12481 agcgagtcat cccagatgtt gctcgtgcgc agggcggcct ggccggcatg ccgcaacacg
    12541 cgtttcgcaa tgaagtgggc cacaagggtg accgtgagca ccagcagcaa acggacgatt
    12601 tcaggactca ggttgaccgg cattttttct cctgaagatt attcgatgat gccgccgccc
    12661 agacaaatgt ccccgtcgta tgccaccacc gactggcccg gcgtgaccgc ccattgcgcc
    12721 tcgtcgaagt cgaagctgca gcggttgtca ttcccgatcg cgatgcggca gggcgcgtcg
    12781 gtctggcgat agcgggtctt ggccgcaaaa gcgcgcgtca gatcgggagc gtgacccgcg
    12841 atccagtgca tgtccagcgc ctccagatgc gggttcagca gggcggggtg gtcgtgcccc
    12901 tgcaccacga tcaggcgatt gttggccatg tctttggcgg cgacgaacca gggctcgccg
    12961 gtcgagtctt tgccgccgcc gatgcccagg ccctgacgct ggccgatggt gtagaacgac
    13021 agccccatgt ggcggccgac gaccttgccc tccggcgtgc gcatttcgcc cggttgagcg
    13081 ggcaggtagc ggttgaggaa ttcccggaac gggcgctcgc cgatgaagca gatgcctgtg
    13141 ctgtctttct tggcatagtt ggacgggccg tgctgttcgg cgatggcgcg cacctcggac
    13201 ttcagcatgt cgccgagcgg gaacatcgcc tttgacagct gcgcctgatt cagccggtgc
    13261 agaaaatagc tctgatcctt gctggcatcc gcagccttga gcagctgaaa caggccatcc
    13321 acttcgcgca cccgcgcata gtggccggtg gcgatactgt ccgcaccgag ctggatcgcg
    13381 tggtcgagga aggccttgaa cttgatctcg gagttgcaca ggatgtccgg gttgggcgtg
    13441 cgcccggatt cgtattcgcg caggaaatag ctgaacacgc ggtccttgta ttccttggcg
    13501 aaattcaccg cctcgatcgg aatgccgatg gtgtcggcga ccgagaccgc atccagcaga
    13561 tcctgacgcg acgagcagta ttcgctgttg tcgtcgtctt cccagttctt catgaacagg
    13621 ccgaccacat catagccctg ctgtttgagc agcaacgcgg tgaccgagga atcgaccccg
    13681 ccggacatgc cgaccacgac gcgccgggat tgaggattgc ttttcacgat agcctcctcg
    13741 cccgcgtgcg ggagaggatt gaggagaggg aatgccgttt agtcatagtg caccagcagg
    13801 tcgagcgggt agcgtttgcc tgcgaggtag tcctcgatgc agcgcaggat cagcgggctg
    13861 cgatggcgtt cttgcgtggc gcggacttcg tctatggtca tccacgctgc gcggacgatg
    13921 ccgtcgtcca gcgcgcgatc cggctcgtgg gcggtgatcg cgccggtgaa ggcgaagcgc
    13981 agataggtga cgttcgaaat atcggaatgc cagcgataga tgccgatcag gtgttgcggt
    14041 gtgaagtgcc aggcagactc ttccagcacc tcgcgcgcgg cgccctgagc cagtgtttcg
    14101 ttcggttccc agtgcccggc gggctggttg aagcgcagcc cctgctcggt ttcttcttcc
    14161 accagcagga aacgcccatc tcgttcgatg actgcggcga cggtgacgtt cggtttccag
    14221 atcatgtcag atcccagaaa gtaggtcagt aaagaaaaaa ggcaggaatt aatcctgcct
    14281 ttcatttgtt ggtgccgggg gggggaatcg aacccccacg ccctctcggg caccggattt
    14341 tgagtccggc acgtctacca gttccatcac cccggcttac tttgcgctac agctgttgca
    14401 aagagcggcg aattatccat gaaacgatgg gcttcatcaa ctacaatcgc aggctcgaaa
    14461 aaattgcatc attgcccatg cgtaccgaag aattcgattt tgacctgccg gaacggctga
    14521 tcgcccagca tccgcccgag cggcgcggcg ccagccgcct gttgtatgcc catgacgggc
    14581 aactagaaga ttgtgtgttt gccgatctgt tgcgtctggt cgggccgaac gatctgctgg
    14641 tgctgaacga cacgcgcgtg atcaaggcgc gcctgttcgg cgaaaaggaa acgggcggca
    14701 agatcgaggt gctggtcgag cgcgtgctgg acgagcacga ggtatgggcg caggtgcgcg
    14761 ccagcaagtc gcccaaggcc ggcacccggc tgctattggc tgactccttg atggtcgagg
    14821 tgctggggcg cgagggcgaa ttctttcatc tgcgctttct ggccgatgag ccggtgctgg
    14881 atctgctgga acgtcacggc cgtctgccgt tgccgcccta tatcacccat gcggcggaag
    14941 ccgcagacga cgagcgttac cagaccgtgt ttgcgcgcga ggccggcgcg gtagcggctc
    15001 ccactgcggg gctgcatttc agtgatgcga tgttgcaggc cctgcgcgac aagggcgtga
    15061 gcatcgcgca tgtcacgctg catgtcggag cgggcacttt tcagccggtg cgtgtggagc
    15121 agatccatga gcacagcatg cacagcgagc gttatgaaat tccgcaggca acggtggccg
    15181 ccatcgcggc gacgcgtgcg gcgggcgggc gggtgatctc tgtggggacc accagcctgc
    15241 gcgcgctgga gagtgcggcc gctgcgggtg aattgaaagc cggctcgggc gagacgcgta
    15301 tcttcatcac ccccggctat cgattccggg tggtggatgt gctgctgacc aacttccatc
    15361 tgccgaggtc cacgctgctg atgctggtga gcgcgttcgg cggcatggcg caaatgcggg
    15421 cggcctatcg ccatgcggtg gcgcaggagt atcgtttctt cagctacggc gatgcgatgc
    15481 tgatcgagcg gctgcgggcc tgaagcgggt gcatgaatct gttagcatgg ccctcaccta
    15541 attcaaggag gcgacatgga accttacgtt ctctggtttt tgctggcgct ggggctgttc
    15601 atcgcagaaa tgatgaccag cacgttttat atgatggtgc tgtccatcgc catgtgcgtc
    15661 ggcggtgtgg cggcgctgat ggacatgagt ccggcggtgc agctggggct cggtgcgatg
    15721 gcgagtatcg tcggtgtggt gctgttgtcg cgctggaagg ctcggcaacc tgcggcgccg
    15781 caagacgacc agcccgatat cggtcagccg gtgcgggtgc tgttctggcg tgaggacggt
    15841 acggcgcgcg tgcattatcg cggcgcggac tgggatgcgg agcccgagag cgccgacatg
    15901 ccgcaggctg gctcgtgcta catcaaggcg ctgcatggat cgatattgat cctgtcgcag
    15961 cacaagccgc aataacgcaa aggagagaga catggaaatc gcactgttta tattgatcgg
    16021 cgccttcatc ttcatcatca aggcgctcaa ggtggtgccg cagcagaacg cctgggtggt
    16081 cgagcgcctg gggcgctttc acgttacgct gacgccgggg ttgagcatcg tggtgccgtt
    16141 tgtcgatcgc gtggcctaca ggcactcgct caaagagatt ccgcgagatg tgcccagcca
    16201 gatctgcatc accaaggaca acacccagct gcaggtggac ggtattctgt actttcaggt
    16261 gacggatccc aggctggcgt cctacggtac cagtgactat gtcttcgcca tcacccaatt
    16321 ggcccagacc acgctgcgct cggtgatcgg caagatggag ctggacaaga ccttcgagga
    16381 acgagacgac atcaaccgtg ccgtggtcgc ggcgctggac gaagcggctt ccagctgggg
    16441 ggtgaaggtg ttgcgttacg agatcaagga tctgacgccg cccaaggaaa tcctgcacgc
    16501 catgcaggcg cagatcaccg ccgagcgcga gaagcgcgcc ttgatcgcgg cttccgaggg
    16561 ccgcaagcag gaacaggtca acatcgccac cggtgagcgc gaagccgcga ttctgcgttc
    16621 ggagggcaac aagcaggccg ccatcaacaa tgcccagggc gaggccgaag cgctcaaggc
    16681 ggtggccgac ggtgccgctc aggcgatccg tatggtcgcc gaagctatcg aggcacccgg
    16741 cggcatgaac gcggtcaatc tcaaggtcgc ggaaaaatac gtcgaggcct tcggcaatgt
    16801 cgccaaggaa ggcaataccc tcattctccc cgccaatctc gccgacatgg gcggcatggt
    16861 ggcggcggcg atgagcatcg tgaaagcaca aaaaggttag ttctatggag ttcagtttgc
    16921 acaaacgcga cggtcttgcc cgtcgcggta ccttacagct tgcccatggc aaggtcgaaa
    16981 cccccgcgtt catgccggtg ggaacctacg gcacggtgaa ggcgatgtcg ccggtcgaat
    17041 tgcacgacat cgatgcacat atcgtgctgg gcaacacctt tcatctgtgg ctgcgtcccg
    17101 ggctggaggt gatagcggcg cacggcggcc tgcatcagtt catggggtgg gacggcccga
    17161 tactcaccga ttccggcggc tttcaggtgt tcagtctggg cgcgttgcgc aagattaccg
    17221 aggagggtgt gaagttccag tcgccggtga atggcgacaa gtgcttcctg tcgccggaag
    17281 aatccatgcg catccagaag gtgctgaatt ccgacatcgt gatgatcttc gacgagtgca
    17341 cgccttatcc tgcgagcgag cgcgatgcgg cggattcgat gcgactgtcc ttgcgctggg
    17401 cggcgcgctc caaggctgcg cacgagggca atccgaatgc gctgttcggc atcgtgcagg
    17461 gggggatgta cgagaacctg cgcgacgagt cgatgggcga gctggtgaag atcggtttcg
    17521 acggctatgc catcggcggg ctgtcggtgg gcgaacccaa ggaggacatg ctgcgcatcc
    17581 tcaagcacac cgcgccgcaa ctgccgcagg acaagccgcg ctatctgatg ggcgtcggca
    17641 cgccggagga catcgtggct gcggtgacgc agggcatcga catgttcgat tgcgtgatgc
    17701 cgacgcgcaa cgcgcgcaac ggcatgatgt ttacccgtca tggcgacatc aagatcaaga
    17761 acgcgcagta ccgtctggac acgcgcccgc tggacgagga ttgccagtgc tacacctgcc
    17821 gcaatttttc ccgatcctat ctgcatcatc tgttccggct cggcgagatc ctcggcgcgc
    17881 gcctgaacac tatccataac ctgcactact atcaggatct gatgcgcgac attcgcgctt
    17941 ccatcgaggc gggggagttc gatgaattcg tggcgaagtt tcaccggatg cgcgccggca
    18001 aggcatgagc gggtgcatcc ttcatgttag aatgcgcgcc ttttgattat tctctcggag
    18061 actaaacgat gtttattagt aacgcttacg ctgaaactgc cgctgccgga tcacagggcg
    18121 gcatcatgga atttctgccg ctgatcgcgc tgctcgcggt gttctatttc ctggtgctgc
    18181 gcccgcagag caagcgcgcc aaggaacaca aggcactggt cgaagcgctg cagcgcggcg
    18241 atgaggtcgt taccgtgggc ggtcaggtcg gttcggtcag caaggtgttc gaggaatatg
    18301 tcgccgtgga agtcgccgag aatgtcgagg tgacggtgca gaaatccgcc atccagaacg
    18361 tattgcccaa gggcacgatc aagtccatca agtaattcaa tcccaacgcg tggaaggccg
    18421 tcgtaccggc gacttatcgg tcgtgcgacg gtctttatga ggtcatcatg aatcgctatc
    18481 ccctgtggaa atatctgctg atcctggccg tgttactggc tggcttgatc tataccctgc
    18541 caaactttta cggtgaatcg cccgcagtgc aggtgtcgcc gttgcgagcc agcctgaagg
    18601 ccgatgccgc gctgctggaa cgcgtgacgg gcatactccg ggaagcgggg ctgaagcccg
    18661 aagtggttgc gctggacggc aacagcgtca aggcgcgttt cggcggcacg gacgagcaga
    18721 tcaaggccag ggacttgttg atcgccaagt tgggcgagga ttacatggct gcgctcaatc
    18781 tgctatcgcg ttcgcccagc tggctagccc gctttgcttc gccgatgtat ctgggcctgg
    18841 acttgcgcgg cggcgtgcat ttcctgctgc aggtggatat gaaggctgcg ctggacaagg
    18901 cgctggaggc tgcgtccagc gatatccgca gcgcgctgcg cgagcagaat atcgcttact
    18961 cgggcgtggc tcgcgacggc aatgtgatgg tggtgcgttt ccgcgacgcc gaggcgcgcg
    19021 gcaagggcga ggccgagatc aagcagcgtt tcaatgatta cgatctgtcc gccaaggaag
    19081 aagggagcga cttcctgttg cgcgccacgc tgaagccgga ggcagagcgg cgcattcagg
    19141 agtccgccgt gcagcagaat ctgctgaccc tgcgcaatcg cgtcaacgaa ctcggtgtgg
    19201 ccgagccggt gatccagcag cagggtctgg atcgcgtggt ggtgcagttg cctggcgtgc
    19261 aggacaccgc gcgcgccaag gatattctcg gccgcacggc gacactggaa gtgcgcatgg
    19321 tggatgatga acaccagatc acgccgggtg cggcgctgcc gttcggcacc gaactgttca
    19381 aggatagaga gggtaatcag ctgctggtga agaagcaggt gatcctcacc ggcgagcgta
    19441 tcagcgatgc gcaacccggt ttcgacagcc gtaacaatga acccgccgta cacatcaatc
    19501 tggacaatgt gggggcacgc aagttcaagg acgcgacgcg cgagaacgtg ggcaagcgca
    19561 tggcgatcat cctgttcgag aagggccgtg gcgaggtcgt caccgccccg gtgatccgcg
    19621 aagagatcgg cggcgggcgc gtgcagatca gcggccagat gaatacggtc gaggcgcagg
    19681 acacctcgct gttgctgcgt gccggcgcac tggccgcccc gatggagatc gtcgaagagc
    19741 gcaccatcgg cccgagtctg ggtgccgaga atatccagcg cggcttcgat tccaccaaga
    19801 tcggtttcat catgatcgcg gtcttcatgg gcgtctatta cctgatgttc ggtgccattt
    19861 cggtgctggc gctgggtgcc aatctgctgt tgctggtggc gatgctgtcc atgctgcagg
    19921 ccacgcttac cctgccgggc atggcgggta tcgcgctgac gctgggtatg gcgatcgatg
    19981 ccaacgtgct gatcaacgaa cgcatccgtg aggaactgcg caatggcgtg acgccgcagg
    20041 cggcgatcca tgccggttat gaccgggcct tcagcaccat cctcgactcc aatatcacca
    20101 cgctgattgc gggcgtgacg ctgttcatgt tcgggtccgg cccggtgaaa ggttttgcgg
    20161 tggtgttgtg tctgggtatc ctgacctcga tgttcagcgc gatgctggta tcgcgtgcgc
    20221 tggtgaatct gatctacggc cgtaaggcac gcttgaacaa ggtcgcaatc ggctgaccct
    20281 ctccctaacc ctctcccgct cgcgggagag gggacgtcgg ctctctctcc ccagcgggga
    20341 gagagttgga gagaggggga aattaatggg gaacaaataa catggaatta ttcaagatca
    20401 aacgcgacat cccgttcatg agctacggga agtacaccac cacgatttcg ctgctgacgt
    20461 tcatcgtcgc ggtgttcttt ctctcgacca aggggctgaa tttcggcgtg gatttcaccg
    20521 gcggtacggt gatggaagtg cattacgccc agccggccga catccccaag gtgcgcgaac
    20581 agcttgctgc gacaggccag ccggaggcgt tggtgcagac cttcggcacc agccaggatg
    20641 tgctgatcca gataccgctc aagcagaatc tcaccggagc gaagctctcc gagcaggtga
    20701 tcgccgggtt gcgcgcacag gatgcaacgg tcgagatgcg ccgcgtcgag ttcgtcgggc
    20761 cgcaggtcgg acgcgatctg gtagagaacg gcgcgctggc gctgctgctg gtctctctgg
    20821 gtatcgtcgg ttatctgtgg ctgcgcttcg agtggaagtt cggcttgtcg gcgctgatcg
    20881 ccaacctgca tgacgtggtc atcatcctcg gcttcttcgc cttcttccag tgggagttct
    20941 cgctgtcggt actggcggcg gtgctggcgg tgctgggcta ttcggtgaac gaatcggtgg
    21001 tggtgttcga tcgtatccgc gagaacttcc gctccatgcg caaggctg
//
LOCUS       DASUIV010000072         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_390075, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000072 DASUIV010000000
VERSION     DASUIV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390075"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..206
                     /gene="rrf"
                     /locus_tag="VFW59_02745"
     rRNA            93..206
                     /gene="rrf"
                     /locus_tag="VFW59_02745"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            329..868
                     /gene="msrA"
                     /locus_tag="VFW59_02750"
     CDS             329..868
                     /gene="msrA"
                     /locus_tag="VFW59_02750"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5363162.1"
                     /translation="MAEATFAGGCFWCLEAVFSQLSGVDRVTSGYSGGFNANPSYEQV
                     CSGTTGHAEVVRIYFDPDQISYRQLLEVFFATHDPTTLNRQGADVGTQYRSAIFSHSP
                     EQTAEARALVERLNEAFPPHSPVVTEIVDAPEFFPAEAYHQRYFATNPGQGYCSFVIN
                     PKVASFRRQFAALLRSPDQ"
     gene            complement(849..1685)
                     /locus_tag="VFW59_02755"
     CDS             complement(849..1685)
                     /locus_tag="VFW59_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5363163.1"
                     /translation="MSHFLADDGVKIHVKVSGEGPPIVMLHGWTSSHQEWFPFLHELH
                     KRHSVYRWDARGHGGHSLGNGMPPTAERMARDLANLVEHYGLEKVVAVGHSMGALTLW
                     QYIRDYGTGRLAKLCFIDQSPKLLTEPGWDHGIYGNFGQDKAGELMTWLEEDFAEGVL
                     KLTAFGLNERAKEKYLAGASGWERSRNALRAQNPSPLIACWASLTAADYRDVLQRIDV
                     PSMLVYGSQSNFYRTETAHYVANQIPDAVLHIYEGTDHSPHQWQRERFTRELMDFIDP
                     DS"
     gene            1749..2699
                     /locus_tag="VFW59_02760"
     CDS             1749..2699
                     /locus_tag="VFW59_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5363164.1"
                     /translation="MFDWRDYGNGIVAFDAGYVRPILAAIHVVVEAGRVAFIDTGSND
                     ALPNALAALKKLGLDSAAVDYIILTHIHLDHAGGAGSMMAAFPNARLVVHPRGARHMA
                     EPARLVAGVTQVYGEDYVRRVYGEILPIPAERIIEAPDGHVVSLAGRELLCLDTPGHA
                     RHHICIVDRKTGGIFSGDMFGLSYREHDVEGRPFIFPTTTPTQFEPEAMHASIDRLLS
                     FKPEAIYLTHYSRVGNVAQLGADLHRRLDAMVACALKVRGSGAERHDALKVALTDYLL
                     AELRAHGCPLLQENVLEIWETDLELNAQGLEVWLDSRPST"
     gene            complement(2696..>3438)
                     /gene="corA"
                     /locus_tag="VFW59_02765"
     CDS             complement(2696..>3438)
                     /gene="corA"
                     /locus_tag="VFW59_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX5363165.1"
                     /translation="NYLYVVLHRYEITTAPLSLVQDQISLIIGPDYLLSFQERQSRTF
                     EPVRQRLRSDRGNIRSAGTDMLAYSLLDSVVDSYFAVVEQLDDYAEKLEIEILGHPGP
                     AVLENIHQFKRCVSQLRRNLYPLRELLGSLDREHAHFFAPEIQLYLRDVYDHTVHIIE
                     SLEDLRELATGLLDVHLTTVSHRVNLEVRMLTVVATIFMPATLIAGIFGMNFHQMPWL
                     ERPSGFYYATGLMVMIACIMLVLFWRRR"
BASE COUNT          738 a          929 c         1003 g          768 t
ORIGIN      
        1 agcactgcag caactcatac cgcagtcaca acaacaacct tcctccagtt ttgcgtttgc
       61 cgctctcaac agcgacaaac gaacaagtta cgcttggcga ccatagcgtt tcggacccac
      121 cccttccctt cccgaacggg accgtgaaac gaaaccgcgc cgatgatatt gcacattgcg
      181 tgtgagaaag taggtcatcg ccaggctacc ccataaaccc cccgcagcta catagctccg
      241 gggggttttg ctttgcagac gtagaacgct taaaacagaa gctacttcgc tatactgagt
      301 gctcaaatga tttttgagcg gaaagaacat ggcggaggct acatttgcgg gtgggtgttt
      361 ctggtgcctt gaggctgttt tttcgcagct ttcgggtgtc gaccgcgtaa cctcgggata
      421 cagcggggga ttcaatgcaa acccgagtta tgaacaagtt tgctcgggca ccacggggca
      481 tgccgaggtc gtcagaatct actttgatcc agaccagatt tcgtatcgcc aactgctgga
      541 agtcttcttt gcaacccatg acccgacgac gctgaatcgc caaggggcgg atgtcggaac
      601 gcaatatcgt tcggcgattt tttctcattc cccggaacag actgctgaag cgcgagcgct
      661 ggtggagcgt ttgaacgagg cctttccacc gcattcgcca gtggtaaccg agattgtcga
      721 tgcgccggaa tttttccccg ccgaggctta tcaccagcga tattttgcga ccaatcccgg
      781 gcaaggctat tgttcctttg taataaatcc aaaggtcgcc tccttcaggc gtcaattcgc
      841 tgcactactc aggagtccgg atcaatgaag tccatcaact cgcgggtgaa gcgttcacgt
      901 tgccattgat gcggggagtg atcggtgcct tcatagatgt gaagcaccgc atccggaatt
      961 tgattggcga cgtagtgcgc cgtttcagtc cgataaaagt tgctctggct cccataaacg
     1021 agcattgacg gcacatcaat tcgttgcagg acatcgcggt aatctgcagc agtcagactt
     1081 gcccagcagg cgatcagagg ggatggattc tgggcgcgca gtgcattgcg cgaacgttcc
     1141 cagccgctgg caccggcgag atatttttcc ttagcgcgtt cgttgagtcc gaaagcggtc
     1201 aatttaagta cgccttcggc gaaatcctct tcgagccaag tcatcagttc tcctgctttg
     1261 tcctgcccga aattcccgta gattccatgg tcccaaccgg gttctgtcag caatttcggt
     1321 gactgatcaa taaagcagag cttggccagg cgtccggtac cgtagtccct gatgtattgc
     1381 caaagcgtca aggcacccat cgagtgcccg acggccacta ccttttcgag gccgtagtgc
     1441 tccactaggt tggcaaggtc tctggccatt ctttcggcgg taggaggcat gccatttccc
     1501 aatgagtggc ccccatgtcc gcgggcatcc cagcgatata cgctgtggcg tttgtggagt
     1561 tcgtgcagga atggaaacca ctcctgatgg ctggatgtcc agccatgcaa catgacaatc
     1621 ggggggcctt ccccggaaac cttgacatga attttgacgc cgtcgtcggc aaggaagtga
     1681 gacatggtcg ctgatatcct tgtgaagggc gaagtataat ccgcgcttca atacggatgg
     1741 gtacggaaat gttcgactgg agagattacg gcaatggcat cgtcgccttc gatgcgggct
     1801 atgttcggcc gattctggcg gccattcatg ttgtggtcga ggctgggcgg gttgcgttca
     1861 tcgataccgg cagcaacgat gcgctgccga acgcgctggc agccttgaaa aagctggggc
     1921 tggatagcgc tgcggtcgac tacatcatcc tgacccatat ccatctcgac catgccggcg
     1981 gggccggcag catgatggcg gcgtttccca atgccagact ggttgtccat ccgcgcggtg
     2041 cccggcatat ggccgagcca gcccggctgg ttgcgggcgt tacccaagtc tatggcgaag
     2101 attacgtacg gcgggtttat ggcgagattt tgccgattcc tgcggaaaga atcattgagg
     2161 cgccggatgg ccatgtcgtg tcgctggccg gtcgtgaact gctgtgtctc gatacgccgg
     2221 ggcatgcccg acaccatatt tgtatcgtcg atcgcaagac gggagggatt ttcagcggcg
     2281 acatgttcgg gctctcctac agggagcatg acgtggaagg gcgccccttc atctttccga
     2341 cgaccacgcc gacccagttc gaaccggagg ccatgcatgc ttcgatcgac cgcctgcttt
     2401 cgttcaaacc agaagcgatc tacctgacgc actacagtcg ggtcggcaac gtggcgcagt
     2461 tgggtgccga cctgcatcgc cggctggatg cgatggtggc ctgcgcgctg aaggttcgcg
     2521 gatctggtgc tgagcggcat gacgcactga aggtggcgct aaccgattat ctgctggctg
     2581 agttgcgcgc acatggttgt ccgttgctac aagaaaatgt tctggagata tgggaaactg
     2641 atctcgaact caacgcccaa ggactcgagg tatggctgga tagtcgcccg tcaacttaac
     2701 ggcggcgcca gaacaatacc agcatgatgc aagcgatcat taccatcagg ccggtggcat
     2761 agtagaaccc gctcgggcgc tcgagccagg gcatctgatg gaaattcatc ccgaagatgc
     2821 cggcaatcag cgtcgctggc atgaagatgg tggcgaccac cgtcagcatc cggacttcca
     2881 gattgacgcg atgactgacc gtcgtcaggt gaacatcgag cagtcccgtt gcgagctcgc
     2941 ggaggtcttc gagcgattcg atgatatgaa cggtgtggtc atacacatcg cgcagataga
     3001 gctggatttc cggcgcgaaa aagtgcgcat gctcgcgatc caggctgccg agaagttcgc
     3061 gtaacggata cagattgcgg cgcagttggg atacacagcg tttgaactgg tggatgtttt
     3121 cgagaactgc cggccccggg tgtccgagaa tttcgatttc gagcttctcg gcataatcgt
     3181 ccagctgttc gaccaccgcg aaatagctat cgaccaccga atcgagcagc gaatatgcaa
     3241 gcatgtcggt gccggcactg cggatattcc cccggtctga ccgcaggcgc tggcggacag
     3301 gttcgaaagt tcgtgactgg cgctcctgaa aactgaggag atagtctggc ccgatgatta
     3361 ggctgatctg gtcctgaacc aggctaagcg gcgcagtcgt gatctcgtag cggtgcagaa
     3421 cgacatagag atagttgt
//
LOCUS       DASUIV010000073         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_761585, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000073 DASUIV010000000
VERSION     DASUIV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761585"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..2460
                     /locus_tag="VFW59_02770"
     CDS             109..2460
                     /locus_tag="VFW59_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEX5363166.1"
                     /translation="MVVFAVCFTLGVWWLQQQPSLPGFVWAWALLALPFVLFIPQRAL
                     WLRLLRGGLIALLACGLGFYHAAWQAEQRLAVSLPEAWQGRDIAVTGVVAELPRSHER
                     GQRFRFDVERVLTPEAHVPDHVYLSTYSDNKSPPLVVKAGERWRFTLRLKQPHGSSNP
                     GGFDFEGWMLERNLRATGYVHPKGDNARLDARADGFGYRIETWREAVRDKFSATLGDA
                     PYAGILSALAIGDQDSIPPQQWQVFTRTGVNHLMSISGLHITMLSGLGFSLAYWLWRR
                     STRLTLWLPARKAAALFAVLIALGYALLAGFGVPAQRTVYMVAAVAAALWLNRNFSSG
                     QILALALLGVLIPDPWAVLSAGFWLSFGAVALILYVTSHRIGRPSLWQEYATVQWAMT
                     VGLTPLLIALFQQVSLVSPVANALAIPLVSLIVVPLVLLGAVLPTEVPLWLAHIAMDG
                     TMHFLEWLNALPQAVWIQHAPPAWSIVMAMLGALWLLLPRGFPARWPGALLILPMFLN
                     TPAPPADKTLRLIVFDVGQGLAVAAQTRNHALLYDSGPDFSGEADSGNRILVPALRAL
                     GVERLDGLMLSHDDLDHIGGSASVLQAMPVGWVSSSLPDGHPLFAQPGLVRHCTDGQS
                     WEWDGVRFELLHPGAEEDELGRTSDNELSCVLRIGIGDQHVLLAGDIEKGGERRLIRE
                     HPDALHATLLVAPHHGSKTSSSQDFIAATLPDYTVFTVGYRNRFGHPRAETLQRYADS
                     GSEILRSDEDGAILVEMDARGLHLERYRQTHRRYWTHQPPGKE"
     gene            2520..2975
                     /locus_tag="VFW59_02775"
     CDS             2520..2975
                     /locus_tag="VFW59_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX5363167.1"
                     /translation="MKYIPRHLATLATVAWVGGLWAIGYLAVPILFHAQPDRQLAGML
                     AGQMFGALGYLGMVCGAYLLAYRLATTGRAALRQKLFWAIAAMLLITLLSQFCFQPLM
                     AQLKAQALPLEVTQSEFSGRFRMWHGISSIAYLLQSLLGAALAVKSFRE"
     gene            3053..3679
                     /locus_tag="VFW59_02780"
     CDS             3053..3679
                     /locus_tag="VFW59_02780"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEX5363168.1"
                     /translation="MTTRKTSTKSKAELSKDKVVKIGQAAAQLHRLLKELGHAKGLER
                     SASTMPDARARLSFIDQTMHEASEKTISAVENSLPLTKSTRKSCAKLSKKLSASEDLA
                     HHALVQETISELGEIAASEDAIHHDLMQIMEAQEFRDVAGQMVNKILGAAVEIESILL
                     DILREYAPNHDDSLISHEGLTSGPSVKSGANDVSGQDDVDDLLASMGF"
     gene            complement(3762..>4919)
                     /locus_tag="VFW59_02785"
     CDS             complement(3762..>4919)
                     /locus_tag="VFW59_02785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5363169.1"
                     /translation="PQGRGATGTAIRSGQIQVINASNPGFRLWKELAQEYNLQSMLAI
                     PLLIRGEVYGAFTLYSRQLEAFSESTRDLLVGIASRISVALEAAMDQQQIRLLGSALA
                     AAGNGVMITNPRGIIQWVNPAFARLSGYSKEELVGQTPRVLNSGQQTPEYYRHMWATI
                     SRGETWSNEMVERAKGGGIYTVSQTITPMLDENGGITHFIAIHEDITVQKLSQERIRY
                     MAHFDALTNLPNRVLFYDRLKQALSMARRNQGGLALLFLDLDGFKAVNDSMGHQTGDL
                     LLKEVAERLQQCVRESDTVARLGGDEFTVILNEAHEHHDVAMVAEKIIEVIAQPFDLE
                     GREARIGVSIGIARYSEEANSEDELVKHADQAMYDAKSAGKNTYRFGPAAL"
BASE COUNT          878 a         1652 c         1457 g          932 t
ORIGIN      
        1 agcggcccta gccgcgatac tccggtcaat ccaattgccc atcgcggctc aagccgctcc
       61 cacaaaacgc ctctgagttt tccatgcgac tttgcgctaa tctagcgcat ggtcgtcttc
      121 gccgtttgct tcacgctggg agtgtggtgg ctgcaacaac agccgtcact gcccgggttc
      181 gtctgggcat gggcgctgct cgcgctgccg ttcgtactgt tcatcccgca gcgagccctc
      241 tggttgcgcc tgctgcgcgg cggtctgatc gccctgctcg cctgcggcct gggtttctat
      301 cacgccgcct ggcaagccga gcaacgactc gccgtcagcc tgcctgaggc atggcaggga
      361 cgcgacatcg ccgtgaccgg cgtggtggcc gaactgccgc gcagccacga acgcgggcaa
      421 cgcttccgtt tcgatgtcga gcgtgtgctc acgccggaag ctcatgtgcc cgaccatgtc
      481 tatctcagca cctattccga caacaaatca cccccgctcg tcgtcaaggc gggagagcgc
      541 tggcgcttca cgctgcgcct caagcagccg catggctcct ccaatccggg gggcttcgat
      601 ttcgagggct ggatgctgga acggaatctg cgcgccaccg gctacgtgca ccccaagggc
      661 gacaacgcaa ggctggatgc gcgggccgac ggtttcggct accgcatcga gacctggcgc
      721 gaggcggtgc gcgacaagtt ttccgccacg ctgggcgatg cgccctatgc cggaatattg
      781 agcgcactgg cgataggcga tcaggacagc atcccgccac aacaatggca ggtgttcacg
      841 cgcaccgggg tgaaccacct gatgagcatc tccggcctgc acatcaccat gctctccggt
      901 ctcggcttct cgctcgccta ctggctgtgg cgccgcagca cgcggctcac gctatggctc
      961 cctgcgcgca aagccgccgc gctatttgcg gtactgatcg cgctcggcta cgcactgctg
     1021 gccggtttcg gcgtaccggc gcagcgcacc gtgtacatgg tcgcggcggt ggccgccgcg
     1081 ctgtggctga accgcaactt ctcctccggg cagatactcg ccctcgcgct gctgggcgtg
     1141 ctgatccccg acccgtgggc tgtgctctcg gcgggcttct ggctgtcgtt cggcgcggtc
     1201 gcgctgatcc tgtatgtgac gtcccaccgc atcggacgcc cgtccctgtg gcaggaatat
     1261 gccacggtgc aatgggccat gaccgtcggc ctcaccccgc tgttgatcgc gctgttccag
     1321 caggtgtcgc tggtctcgcc cgttgccaat gcactcgcga tcccgctggt gagtctgatc
     1381 gtggtgccgc tcgttttgct cggcgcggtg ctaccgaccg aagtgccgct gtggctggca
     1441 catatcgcga tggacggcac catgcacttc ctcgaatggc tgaatgcgct gccgcaggcg
     1501 gtatggatac agcatgcgcc gcccgcctgg agcatcgtca tggcaatgct gggtgcgctc
     1561 tggctgctgt tgccgcgcgg cttcccggca cgctggccag gcgcactgct gatcctgccg
     1621 atgttcctga acaccccggc gccaccggcg gacaagacct tgcgcctgat cgtgttcgat
     1681 gtcggacagg ggctggcggt cgccgcccaa acgcgcaacc acgcgctgct ctacgacagc
     1741 gggcccgatt tctccggcga ggccgacagc ggcaaccgca tcctcgtgcc cgcgctgcgc
     1801 gcactcggcg tcgaacggct ggatggcctg atgctcagcc atgacgatct cgatcacatc
     1861 ggcggcagcg cctcggtgct gcaggcgatg cccgtcggct gggtctcctc ctctctgccc
     1921 gacggccacc cgctattcgc tcagcccggg ctcgttcggc actgcaccga cgggcagagc
     1981 tgggaatggg acggcgtgcg cttcgagctg ttgcatccgg gcgcggaaga agacgagctc
     2041 ggcagaacat ccgacaacga gctgagctgc gtgctgcgca tcggcatcgg cgaccagcac
     2101 gtgttgctgg caggcgacat cgagaagggc ggcgaacgga gactgatccg tgaacatccc
     2161 gacgcgctgc acgccacgct gctggtcgca ccgcaccacg gcagcaagac ctcatccagc
     2221 caggacttca ttgccgccac cctgcccgat tacaccgtat tcaccgtcgg ctatcgcaac
     2281 cgcttcggcc acccgcgcgc agagacgctg caacgctacg ccgacagcgg ctcggaaata
     2341 ctgcgctcgg acgaggacgg cgcgatcctg gtcgagatgg atgctcgtgg attgcatctg
     2401 gagcgctacc gccagacgca tagacgctac tggacgcatc agccgccggg caaggaatag
     2461 cccgccatca cgggttccga attggtatca ttcacccttg ttttttcaac atcggcgcca
     2521 tgaaatacat tccccgtcat ctcgctacgc tggccaccgt cgcctgggtc ggcggcctat
     2581 gggccatcgg ctatctcgcc gtgcccatcc tgttccatgc ccaacccgac cggcaactgg
     2641 ccggcatgct ggccgggcag atgttcggcg cgctgggcta tctgggcatg gtgtgcggcg
     2701 cctatctgct ggcttatcgc ctggcgacaa ccggccgggc cgcgctccgg caaaagctgt
     2761 tctgggccat cgccgcgatg ctgctgatca cgctgctgtc gcaattttgc ttccagccgc
     2821 tgatggctca actgaaggca caggctctgc cgctggaggt gacgcaaagc gaattttctg
     2881 gccgcttcag gatgtggcac ggcatctcca gcatcgccta tctgctgcaa agcctgctgg
     2941 gcgcggcact ggccgtcaaa tcgttccggg aataagccat gacgaagcaa ctgttcgcca
     3001 cactgcccgc gactttgtta tcatttcgaa ccgattttga gaggactgac cgatgaccac
     3061 gcgcaagacc agcaccaaat ccaaagccga gctctcgaaa gacaaagtgg taaagatcgg
     3121 ccaggccgca gcgcaactgc accgcctgct gaaagaactg ggccacgcca aaggactgga
     3181 acgcagcgca tccaccatgc cggatgcgcg cgcccgtttg agcttcatcg atcagaccat
     3241 gcacgaagcc tcggagaaga ccatcagcgc ggtggagaat tcgctgccgc tcaccaaatc
     3301 cacgcgcaag agctgtgcca aattaagcaa gaaactgtcg gcgtcggaag atctcgcaca
     3361 tcacgcgctg gtgcaggaaa cgatctcgga actgggcgag atcgccgcct ccgaagatgc
     3421 gatccaccac gacctgatgc agatcatgga agcgcaggaa ttccgcgatg tcgcgggtca
     3481 gatggtcaac aagattctgg gcgcggcggt agagatcgag agcatcctgc tcgacatcct
     3541 gcgcgaatat gcccccaatc acgatgattc gctgatctcg cacgaaggcc tcacctccgg
     3601 cccctccgtc aagagcggcg ccaacgacgt cagcgggcag gacgatgtgg acgatctgct
     3661 cgcttcgatg ggattctgaa gccggtaccg atgaatgaaa aaaccgcccg atcgggcggt
     3721 tttttcatgg ctgccagata ggcggcgatc cgcaccgcac actacaacgc ggcgggacca
     3781 aaacggtagg tattcttgcc cgccgacttt gcatcgtaca tcgcctgatc ggcatgtttc
     3841 accagctcgt cctcgctgtt ggcctcttcc gaataacgcg cgatgccgat gctgacaccg
     3901 atgcgcgcct cacgcccctc cagatcgaag ggctgcgcga tcacctcgat gatcttctcg
     3961 gcgaccatcg cgacatcgtg atgctcgtgc gcctcattga gaatgacggt gaattcatcg
     4021 ccgcccagac gcgccaccgt atcgctctcg cgcacacact gctgcaggcg ctccgccacc
     4081 tccttgagca acagatctcc ggtctgatgc cccatggaat cgttcaccgc cttgaagccg
     4141 tccaggtcga ggaacagcaa cgccagccca ccctgattgc gccgggccat cgacaacgcc
     4201 tgcttcaggc ggtcatagaa cagcacgcga ttcggcagat tggtcagcgc atcgaaatgc
     4261 gccatgtaac ggatgcgttc ctgcgacagt ttctgaaccg tgatgtcctc gtgaatggcg
     4321 atgaaatgag tgatgccgcc gttctcgtcc agcatcggcg tgatggtctg cgaaaccgtg
     4381 tagatgcccc ctcccttggc tcgctctacc atctcgttgc tccaggtctc ccccctggag
     4441 atcgtcgccc acatgtgccg gtaatactct ggcgtctgct gccccgaatt cagcactctc
     4501 ggggtctgcc cgaccagctc ttccttgctg taaccgctca gccttgcgaa ggccggattg
     4561 acccactgga tgatgccgcg cggattggtg atcatcacgc cgttgcccgc cgccgccaac
     4621 gcggaaccca gcagccggat ctgctgctga tccattgcgg cctcgagcgc cacgctgatg
     4681 cgcgaggcga taccgaccag cagatccctc gtactctcgc tgaaagcctc cagttgtctg
     4741 gaatacaggg tgaaggcgcc atagacctcg ccgcgtatca gcagcggaat cgccagcatg
     4801 gactgcaggt tatattcctg cgccagctcc ttccacaacc tgaaacccgg attggacgcg
     4861 ttgatgacct gtatctggcc gctgcgtatc gccgttccgg tggcgcctcg tccctgcgg
//
LOCUS       DASUIV010000074         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_947314, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000074 DASUIV010000000
VERSION     DASUIV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947314"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2312
                     /locus_tag="VFW59_02790"
     CDS             <1..2312
                     /locus_tag="VFW59_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5363170.1"
                     /translation="AEIGTHDLALALERELVGQIQSEEMALLSVQDELYRQRARGRVD
                     AAALNAYMERVHSRLPEIDAIRIADERGMLVYGTDVRSADKISLSDRPHFIKLREEAN
                     AGLVISRPQISRVNNKAVIVLARRLEHQDGSFGGMVFATVPLANLTSIFSALNVGERG
                     VVAMRDDELALVASFPLHAMAIDGKSAAAELQLRLQAGQAFGTYRTQDDKIERTVSFR
                     KVGDYPFFVIVGRASGDYLADTNQTVKVILGLLVLFFLVTLLLANMAYRSWLRQLRLN
                     QSLLESETRLDELFENMSSGVAVYRVSADGETFSFSGLNRAAEKFDNSLREDLLGKNV
                     VDVFPGIVELGLLEVFKRVWKTGIPERYPLSFYHDGRISGWRDNFVYKLPSGEIVAIY
                     DDVTERMQAQLALEELNRDFVSLLENTSDFIYFKDKDSRIRFCSQSMALITGHRNWHE
                     LAGKHDQEIFPPETAKLYSEEELPIFRDGVPLLNKTDPYFDAQGNRRWVNANKWPVFD
                     SDGKTVIGLFGISRDITERQKIEDALQRSNADLERFAYSVSHDMRQPLRAVSGHLQLL
                     QRSLKDKLDEDERMNLDFALEGARRMDSMIVSLLDYSRVGRKTESKQWIESRASVDEA
                     LHFLAPLIAESGADVSLNGEWPKVFASRDEMTRLFQNLVGNAIKFRESGQSPVVEVDS
                     SLHDDSWRVSVRDHGVGIDPQQIDRLFQFFSRLQSRVRFDGTGMGLALCRRIVEHHNG
                     RIWVESEGVGCGSVFIFELPLKTMEASAA"
     gene            2309..4198
                     /locus_tag="VFW59_02795"
     CDS             2309..4198
                     /locus_tag="VFW59_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5363171.1"
                     /translation="MNESGVWGNELSVARALTWRYVIALSLVATLSTAAWISLHLVIT
                     EQKSTAAVVNVSGRQRMLSQRTALFSNLLLTSSKSERAIIRGKLKDAINLMERSHHGL
                     LHGDAEMGLPETMSHSVHALYFDGANPLNDQVEAYVDAVKSLLQTDDDALTPDNALLQ
                     YITRTAPGTLLSALDRMVSQYQSEGEASVGRLQKAETAFWLITLLLLMLEALLIFHPF
                     VTHVRSIIGRLQSVTGQLRSHQDQLEEQVKKRTQDLENRGRELAESEEKFRLISTAAQ
                     DAIVIIGTQEQVVYWNPAAEKIFGYKAEEVMGQNMHDLLAPLPQRDLAHQGFSGFQHS
                     GTGALVGKTFEVNALRKSGVEFAAELSISAIELQGSWHALGIIRDITERKAIELHLQE
                     KTQALLRSNTDLEQFAYSVSHDMRQPLRAVAGHLQLLQLNLKDKLDEENRENLAFALS
                     GARRMDSMIVSLLEYSRVGRKTDAKQWIQSRMVLDEALEFLASSIRQTNAEIQINGAW
                     PLVFASSDELLRLFQNLIGNALHYHESEQVPRIEIDSGLHARTWRVSVRDHGIGIDPR
                     QIDRLFQFFSRLQLRERFDGTGMGLALCKRIVEHHDGRIWVESEGEGRGSTFIFELEL
                     PEHEA"
BASE COUNT          902 a         1107 c         1340 g          912 t
ORIGIN      
        1 aggccgagat cggaactcac gatctggcgc tggcactgga aagagaactg gtaggccaga
       61 tccagtcgga agagatggcg ctgctcagtg tgcaggacga actgtaccgg cagcgggcgc
      121 gcggcagggt cgacgccgca gcactcaatg cctatatgga gcgggtgcat tcgcgtttgc
      181 ccgagatcga tgccattcgc atcgccgacg agcgcgggat gctggtgtac ggcacggatg
      241 tccggtcggc cgataaaata tcgctgtccg accgccctca cttcatcaag ttgcgagaag
      301 aggccaatgc ggggctggtg atttccagac cgcagatcag ccgcgtgaat aataaggccg
      361 tcatcgtgct ggcgcgccgc ctggagcatc aggatggatc attcggcggc atggtatttg
      421 ccaccgttcc gctggcgaat ctgaccagca tcttttcggc tctgaatgtg ggcgagcgcg
      481 gcgtggtggc gatgcgcgat gacgagttgg cgctggtcgc aagttttccc ttgcacgcga
      541 tggcgataga cggcaagtct gcggcggctg agttgcagct gcggttgcaa gcggggcaag
      601 ccttcggtac ttaccgcact caggacgaca agatcgagcg cacggtctcc tttcgcaagg
      661 taggggatta tccgttcttt gtcatcgtgg gacgagccag cggtgattat ctggccgata
      721 ccaatcagac cgtgaaggtg atcctggggc tgctggtgtt gttttttctc gtcacgctgc
      781 tgctggcgaa catggcctat cgatcatggt tgcggcagct caggttgaat caatccctgc
      841 tggaaagcga aacccgtctc gacgaactgt tcgagaacat gagcagcggc gtcgccgttt
      901 atcgggtatc tgcggacggg gagacctttt cgttcagcgg gttgaatcgc gcggcggaaa
      961 aattcgacaa ttccctgcgt gaagatttgc tcggcaagaa tgtggtcgac gtgtttcccg
     1021 gtatcgtcga gctgggtttg ctggaggtgt tcaagcgggt gtggaaaacc ggaattccgg
     1081 agcgctaccc cctgtccttt taccacgacg ggcgcatctc cggctggcgg gataattttg
     1141 tatacaagct gcccagcggc gagatcgtgg cgatctacga cgatgtcacc gagcgcatgc
     1201 aggcgcagct ggcactggaa gagctgaacc gggatttcgt cagcttgctg gagaatacct
     1261 ccgactttat ctacttcaag gacaaggaca gccgtatccg tttctgcagt cagtcgatgg
     1321 cgctgataac cgggcatcgg aactggcatg aactggcagg caagcatgac caggaaatct
     1381 tcccgccgga gaccgcaaag ttatattccg aagaagagtt gccgatattc cgcgatggtg
     1441 tgccgctgtt gaacaagacc gatccctatt tcgatgcgca gggtaaccgg cgctgggtca
     1501 atgccaacaa atggccggtg tttgatagcg atggaaaaac ggtgatcggc ttgttcggta
     1561 tcagccgcga catcaccgaa aggcagaaga tcgaggatgc attgcaacgc tcgaacgccg
     1621 atctcgagcg cttcgcctat agcgtttcgc acgacatgcg ccaacccttg agggcggtca
     1681 gcgggcattt gcagttattg caacgcagcc tgaaggacaa gctcgacgaa gacgagcgca
     1741 tgaatctgga tttcgccctg gagggcgcgc gccggatgga ttcgatgatc gtctcgttgc
     1801 tggattattc gcgcgtcggg cgcaagaccg agtccaagca atggatagag agccgggctt
     1861 ctgtcgatga ggcgctgcat ttcctcgcac ccctcatcgc cgagtccggc gccgatgtga
     1921 gcttgaacgg tgaatggccg aaagtctttg ccagtcgcga cgagatgacg cggttgttcc
     1981 agaatctggt cggcaatgcc atcaagttcc gcgaatcggg gcagtcaccc gtggtcgaag
     2041 tcgattcctc gctgcatgac gactcatggc gggtgagtgt gcgggatcac ggcgtgggta
     2101 tcgatccgca acaaatagac cgcctgtttc agtttttcag ccggttgcag tcgcgagtgc
     2161 gcttcgatgg gacgggcatg gggctggcat tatgccgccg catcgtcgag catcataacg
     2221 gccgcatctg ggtagaatcg gagggggtgg ggtgtggcag cgtattcatt tttgaactgc
     2281 cgctcaaaac aatggaggcc agcgcagcat gaacgaatcc ggcgtatggg ggaatgaact
     2341 gagcgttgca cgcgcactga cctggcgtta tgtcatcgcc ttgtccttgg tggcgacgct
     2401 ttcaacggct gcctggatca gcctgcatct ggtcattacg gagcagaaga gcactgcggc
     2461 cgtggtcaat gtgagcggtc ggcaacgcat gcttagccag cgaaccgcat tgttttccaa
     2521 tttgttgctg accagttcca agtcggagcg ggcgatcatt cgcggcaagc tgaaagatgc
     2581 gatcaacctg atggagcgct cgcatcatgg cctgctgcac ggcgatgccg aaatgggatt
     2641 gccggagacc atgtcccact cggttcatgc gctgtatttt gacggtgcca atccgctcaa
     2701 cgatcaggtt gaagcttatg tcgatgcggt caagagcttg ctgcagacgg acgatgacgc
     2761 gctgacgccc gacaacgcgc tgttgcaata catcacccgg acggcgccgg gcaccttgct
     2821 ttcggcactt gaccggatgg tcagccaata ccaatccgag ggggaagcct ccgtcggacg
     2881 cttgcaaaaa gccgaaacgg cattctggct gattaccctg ttgctgttga tgctcgaagc
     2941 cctgctgatc tttcacccct tcgtcactca cgtccgatcc atcatcggca ggttgcaatc
     3001 ggtgacgggg cagttgcgtt cgcatcagga ccagctggaa gagcaggtca agaaacgcac
     3061 gcaggatctg gaaaaccgtg gcagggagct ggccgagagc gaagagaaat ttcgcctgat
     3121 cagtacggct gcacaggatg cgattgtcat catcggtacg caagagcagg tagtctattg
     3181 gaatcctgcc gcagagaaga tattcggcta caaggcagag gaggtgatgg ggcagaatat
     3241 gcatgatttg ctcgcccccc ttccgcaaag agacttggcg catcagggct tcagcgggtt
     3301 tcagcattcc ggcacagggg cgctggtcgg caagaccttt gaagttaacg cgctgcgcaa
     3361 gagcggcgtg gagttcgctg ccgaactttc catctctgcc atcgagttac agggtagctg
     3421 gcatgcgctg ggtatcattc gggatatcac cgagcggaaa gcgatcgaac tgcatttgca
     3481 ggaaaagaca caggcattgt tgcgttccaa caccgatctt gagcagtttg cctacagcgt
     3541 gtcgcatgac atgcgtcagc ccttgcgtgc cgttgccggt catttgcagc tgttgcagct
     3601 gaacttaaaa gacaagctgg atgaggaaaa tcgggagaac ctcgcattcg ccttgtctgg
     3661 cgcgaggcgt atggactcga tgatcgtctc gttgctggag tattcgcgcg tcggacgcaa
     3721 gacggatgcc aaacagtgga tacagagccg gatggtgctg gatgaggcgc tcgaatttct
     3781 cgcgtcctcg atacgccaga ccaacgcgga aatccagatc aatggcgcat ggccattggt
     3841 gtttgccagc agcgatgaat tgctgcgtct gttccagaac ctgatcggga atgcgctgca
     3901 ctatcacgaa tcggagcagg tgccgcgcat cgaaattgac tcgggcctcc atgcacggac
     3961 ctggcgtgtc agcgtgcgtg accatgggat aggaatcgat ccgcgtcaga tcgatcgtct
     4021 gttccagttt ttcagccgct tgcaactgcg ggagcgtttt gatgggacgg gcatggggtt
     4081 ggcattgtgc aaacgcatcg tcgagcatca cgatggccgt atctgggtgg aatcggaagg
     4141 ggaagggcgg ggcagcacat tcatctttga gctggagttg cccgaacatg aggcctgaca
     4201 gagccgaact tgccgcgaac aaagcgtgac agcatggtaa tatttaccgg cgtgtgtctc
     4261 g
//
LOCUS       DASUIV010000075         5129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1040947, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000075 DASUIV010000000
VERSION     DASUIV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040947"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFW59_02800"
     CDS             <1..226
                     /locus_tag="VFW59_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363172.1"
                     /translation="RNVAAYLSNGPVPAAFLVLAVLSVLSSLLPNQPPTVFLASLTVE
                     LTEEQPARADNNTHNAIRRDNEFISLPSGI"
     gene            complement(233..826)
                     /locus_tag="VFW59_02805"
     CDS             complement(233..826)
                     /locus_tag="VFW59_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5363173.1"
                     /translation="MIKEYSPLQSTPLKTGSSFVRPVQALPEKVKLSDAATDVMTDLS
                     KVSVVSVRAKTAMDKANAKMIKYGVRMLLVLDDNEQVVGLLTAADVMGEKPMRFLQNM
                     GGTHDDIMVRDIMTTQRELEVLKVEDVRKATVGQIVSTLKKANRQHALVVAEAADGRQ
                     SVCGLFSITQIARQLGAQVQSFELARTIAEIEAVIAH"
     gene            complement(861..1379)
                     /locus_tag="VFW59_02810"
     CDS             complement(861..1379)
                     /locus_tag="VFW59_02810"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX5363174.1"
                     /translation="MIDRDGYRPNVGIIITNDRNQVFWGKRVRQHAWQFPQGGIQHGE
                     TPEQAMYRELHEEVGLLPEHVKILGRTREWMRYDVPQSWSKRESRGGYRGQKQIWFLL
                     RLTGRDCDVCLRASEHPEFDAWRWNDYWLDINAVIEFKREVYTLALKELVKYLPERRY
                     KSGFVQQISRSC"
     gene            1522..3231
                     /locus_tag="VFW59_02815"
     CDS             1522..3231
                     /locus_tag="VFW59_02815"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5363175.1"
                     /translation="MRVSQFFLSTLKEAPSEAELPSHKLMMRAGYIKKLASGLYTWMP
                     LGLRVLRKVENIVREEMNKSGGIELLMPAVQPAELWQETGRWAVFGPQMLKITDRHNN
                     SFCFGPTHEEVITDIARREIRSYRQLPVNFYQIQTKFRDEVRPRFGVMRAREFMMKDA
                     YSFHADFASLEQTYGVMYDTYSRIFTRLGLKFRAVAADTGAIGGSGSHEFHVLADAGE
                     DALAYCPDSDYAANVELAEAVVPTTARAAATQSLQNVDTPKQTTCEQVAELLGIPLQR
                     TVKLLALIADGTLQILLLRGDHSLNEVKASKLPGLTDFRFANDEEIRAFFNCPAGFLG
                     PVGIDRTRVRVIADRSVAVMSDFVVGANLPKFHTTGVNWGRDLPEPDLVADIRNVVSG
                     DTSPDGKGTLEICRGIEVGHIFQLRTKYAEALNATFLDAQGKTQIMEMGCYGIGVSRI
                     VAAAIEQNFDERGIALPAAMAPFQLVIVPIGMGKSEAVRSAVEQLYADCQAAGIEVLL
                     DDRDERPGVMFADMELIGIPQRIVVGDRGLKEGNVEYQGRQDAAAQVIPLQSVLKLVE
                     SKA"
     gene            3228..3950
                     /locus_tag="VFW59_02820"
     CDS             3228..3950
                     /locus_tag="VFW59_02820"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5363176.1"
                     /translation="MKTGYGVQNTGHSEGAKASLRFVARVPLNPVSRILYSASLAFAL
                     CLPLDASAGAQQEEALAASVRAVLQHSVSDLAPPKLAFASKSEADRWLGEMSPRLQKR
                     IPDAEARVDFLSTVHYEATRAGLDPQLVLGLIEVESGFKKYAVSEVGARGYMQVMPFW
                     VKNIGARDQNLFHLRTNLRYGCTILRHYLDIERGSLYRALGRYNGSLGRPEYPNLVRA
                     AWRNHWSLPQQTVSNDNSTRPL"
     gene            complement(3964..4431)
                     /locus_tag="VFW59_02825"
     CDS             complement(3964..4431)
                     /locus_tag="VFW59_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5363177.1"
                     /translation="MTEWWSRLSAGLLLAVSMSVCAADFELKDISGKSHRLADYRGKW
                     VLVNFWATWCPPCLNEMPELVSLHSAHKDHDLVVIGVALDSTRASVVEFAAKENISYP
                     LVLGNHRMAAQIGEVEVLPTSFLYAPNGKLVASQSGEVTRESIESYIRDKRSN"
     gene            complement(4460..5035)
                     /locus_tag="VFW59_02830"
     CDS             complement(4460..5035)
                     /locus_tag="VFW59_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEX5363178.1"
                     /translation="MGFLEKLFEGALWNSRFMVLTAVVGSLLAGIAIFYMATVDVVNL
                     FGHALHYADSSLTVEARKELHDATVSHIVEVVDGYLLATVMMIFSLGMYELFISDIDQ
                     AHGTKSSSKILVINSLDDLKSRLAKVILMIMIVTLFEEALNMHLETPLDLVYLGAAIA
                     LISLALYFTHASDPAGARAAEDGDAPEKHGH"
BASE COUNT         1135 a         1606 c         1362 g         1026 t
ORIGIN      
        1 tcgcaatgtc gcggcgtact tgagcaacgg gccagtcccc gccgcctttc tggtgttggc
       61 ggtgctaagt gtgctgtcat cactgctgcc gaaccagcca cccaccgtat ttttagcatc
      121 attgacggta gaactgaccg aagaacagcc ggcaagagca gacaacaaca cacacaacgc
      181 gatacgccgc gacaacgaat tcatcagctt accctcggga atttgactgc gattaatggg
      241 caatcactgc ctcgatctcg gcgatggtgc gtgccaactc gaaactttgc acctgcgctc
      301 ccaactggcg agcgatttgc gtgatggaga acaagccaca aacagactgt ctgccatccg
      361 ccgcttcagc cacgaccagc gcatgctggc gattcgcctt cttcagggtg gaaacgatct
      421 gaccgaccgt cgctttgcgc acatcttcaa ctttcagcac ttccagctca cgctgcgtgg
      481 tcatgatgtc gcgcaccatg atgtcgtcat gcgtcccgcc catgttctgc aggaaacgca
      541 tgggcttttc acccatcaca tcggcagcag tcaacaggcc gaccacctgc tcgttatcgt
      601 ccagcaccag cagcatacgc acgccgtatt tgatcatctt ggcattggcc ttgtccatcg
      661 cagtcttggc acgcacgctg accacagaga ccttgctcaa atcggtcatc acgtcggttg
      721 ccgcatcgct caacttaact ttctcgggca gagcctgaac cggacgcacg aaggaagaac
      781 ctgtcttcaa tggcgtgctt tgcaatggac tgtattcttt aatcatcact tcactcctta
      841 acaatctaaa taaacaatta tcaacacgat ctggaaattt gctgcacgaa cccgctcttg
      901 tacctgcgtt ccggcaaata tttcaccaat tccttcagcg ccagcgtata cacctcacgc
      961 ttgaattcga tcacggcatt gatatccagc cagtaatcgt tccagcgcca agcatcgaat
     1021 tcgggatgct cggatgctcg cagacagaca tcacaatcac gaccggtcag gcgcaacaga
     1081 aaccatatct gcttctggcc gcgatagccg ccgcgcgatt cgcgcttgct ccaactctgc
     1141 ggaacatcgt agcgcatcca ttcgcgggta cgaccgagta tcttcacatg ctctggcagc
     1201 agaccaactt cctcgtgcaa ctcgcggtac atcgcctgct ccggtgtctc gccgtgctgt
     1261 ataccgcctt ggggaaactg ccacgcatgc tgtcgcaccc gcttgcccca gaacacctga
     1321 ttcctgtcgt tcgtgataat gatgccgaca ttcgggcggt agccgtctcg gtctatcatt
     1381 tgctgctcta tccggttaat ttcaacgccg ggattctttc acatttcccc actatttgaa
     1441 agcgaatcag ccgcaatcgt accgaatagc gcgcattccc tgtaaaatcg cggcctcgtt
     1501 ttttatccgg atcgtcacat catgcgcgtt tcacaatttt tcctctccac cctcaaggaa
     1561 gccccttctg aagccgagtt gcccagccac aagctgatga tgcgtgccgg ttatatcaaa
     1621 aagctggcca gcggcctgta tacctggatg ccgttggggc tgcgcgtact gcgcaaggtg
     1681 gagaacatcg tgcgcgagga gatgaacaag agcggcggta tcgagttgct gatgcccgcc
     1741 gtgcaacccg ccgaattgtg gcaggagacc ggacgctggg ccgtgttcgg cccgcagatg
     1801 ctgaagatca cggacaggca caacaacagc ttctgcttcg gccccaccca cgaggaagtg
     1861 atcaccgaca tcgcacggcg cgagattcgc agctaccgcc agttgccggt caacttctac
     1921 cagatccaga ccaaattccg cgacgaggta cgtccacgtt tcggcgtgat gcgcgcacgc
     1981 gaattcatga tgaaggacgc ctattcgttc catgccgact tcgccagcct ggagcaaacc
     2041 tacggcgtga tgtacgacac ctacagccgc atctttaccc gtctgggcct gaagttccgc
     2101 gccgtggcgg cggacaccgg cgcgatcggc ggttccggct cgcacgagtt tcacgtgctg
     2161 gccgatgcgg gcgaagatgc actggcctac tgcccggatt cagactatgc ggcaaacgtg
     2221 gaactggccg aggccgttgt gcccaccacc gcgcgcgccg ctgcaaccca gtccctgcaa
     2281 aatgtagata cgccgaaaca gaccacctgc gaacaggtcg ccgaactgct gggcatcccg
     2341 ttgcagcgta ccgtcaagct gctggcgctg atcgcggatg gcacgctgca aatcctgctg
     2401 ctgcgcggcg accacagcct gaacgaagtc aaagccagca aactgccggg actgaccgac
     2461 ttccgatttg caaatgatga agaaatccgc gcattcttca attgtccggc gggcttcctc
     2521 ggcccggtcg gtatcgaccg cacccgggtt cgcgtcattg ccgaccgctc cgtcgccgtc
     2581 atgagcgact tcgtggtggg cgcgaacctg cccaagttcc ataccaccgg ggtgaactgg
     2641 ggtcgcgacc tgccggagcc cgatctggtc gccgacatcc gcaacgtcgt ctcaggcgac
     2701 acctccccgg acggcaaagg tacgctggaa atctgtcgcg gcatcgaagt cggccatatc
     2761 ttccagctgc gcaccaaata cgccgaggcg ctgaacgcca ccttcctcga tgcacagggc
     2821 aagacacaga tcatggaaat gggctgctat ggcatcggcg tgtcgcgcat cgtggcggcg
     2881 gccatcgagc agaacttcga cgagcgcggc atcgcattgc cggccgcgat ggcgccattc
     2941 caactggtca tcgtgccgat cgggatgggc aagagcgagg ccgtgcgcag cgccgtcgag
     3001 cagttgtacg cggactgcca ggccgccggc atcgaagtgc tgctggatga tcgcgacgaa
     3061 cgtcccggcg tgatgtttgc cgacatggag ctgatcggca tcccgcaacg catcgtcgtc
     3121 ggcgaccgtg gactgaaaga aggcaacgtg gaatatcagg gccgacaaga tgccgcagcg
     3181 caggttatcc ccttgcaaag cgtactgaaa ctcgtagaat caaaggcatg aaaacaggat
     3241 atggggtgca gaacacagga cacagcgagg gagcgaaggc gtcccttcgc ttcgttgcgc
     3301 gcgtccccct gaaccccgta tcccgcatcc tgtattccgc atccttggca ttcgccttgt
     3361 gtctgccgct cgacgcatcg gctggcgcgc agcaggaaga ggcgctggct gccagcgtac
     3421 gcgccgtgtt gcaacacagc gtgtccgatc tggccccccc caagctggcc ttcgccagca
     3481 aatccgaagc cgataggtgg ctaggcgaga tgtcacctcg tttgcagaag cgcataccgg
     3541 atgctgaggc gcgtgtggat tttttgagca cggtgcatta cgaggcgacc cgcgcgggac
     3601 tggatccgca actggtgctc gggctcatcg aagtggaaag cggcttcaag aaatatgccg
     3661 tatccgaggt cggtgctcgc ggctacatgc aggtgatgcc attctgggta aaaaacatcg
     3721 gcgcccgcga tcaaaacctg ttccatctgc gcaccaatct gcgctacggc tgcaccatcc
     3781 tgcgccacta cctggacatc gagagaggga gcttgtatcg cgcactggga cgctacaacg
     3841 gcagcctggg gcgtcctgaa tatcccaatc tggtacgcgc agcatggcgc aaccattggt
     3901 cgctgccgca acaaaccgtg agcaacgaca actcgactcg cccgctttaa gccatgcgag
     3961 cggtcagttc gagcgcttgt ccctgatgta agactcgatg ctctcgcgag tcacttctcc
     4021 cgactggctg gcaaccaact tcccgttcgg cgcataaaga aaactggtag gcaagacttc
     4081 cacttcgccg atctgcgccg ccatccggtg gttacccaga acaagcgggt agctgatatt
     4141 ttctttggcg gcgaactcga cgaccgatgc acgcgtagag tccaacgcca cgccgatcac
     4201 caccagatca tggtccttgt gcgcgctgtg cagtgacacg agttcaggca tttcattcag
     4261 acaaggcgga caccaggtcg cccagaaatt caccagcacc catttgccgc gatagtccgc
     4321 cagacgatgg gacttaccgg agatatcctt cagctcgaag tccgcagcgc aaaccgacat
     4381 ggagacggcc agcaacagac cggccgacaa gcggctccac cattcagtca tgactgcggc
     4441 ttaatgaccg tgcttcgact cagtgaccgt gtttctctgg cgcatcacca tcctctgcag
     4501 cgcgggctcc ggccgggtca gatgcgtgag taaaatacag cgccagcgag atcagcgcga
     4561 tggcagcgcc cagatagacc agatccagcg gcgtctcgag atgcatattc agcgcctcct
     4621 cgaacagcgt gacgatcatg atcatcagga tcaccttggc cagacgcgac ttcaggtcgt
     4681 ccagactatt gatcaccaga atcttgctgg aagatttcgt accgtgcgcc tgatcgatat
     4741 cgctgatgaa cagttcatac atacccagcg aaaagatcat catcaccgtc gccagcagat
     4801 aaccgtccac cacttccacg atatgcgaca ccgtggcgtc atgcagctcc ttgcgcgctt
     4861 cgacagtcag actggagtcg gcataatgca gcgcatgtcc gaacaggttc accacatcca
     4921 cggtcgccat atagaagatc gcgataccgg ccagcagact acccaccact gccgtcaata
     4981 ccatgaagcg gctattccac aacgcgccct cgaataattt ttccaaaaaa cccatgtcgt
     5041 actcctaaca ataaaaacgt tgcgcattaa aacgtaaatg ctccgcttgg tgcaagctca
     5101 tcaggcagcg cttcgcgcaa atagttcct
//
LOCUS       DASUIV010000076         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_111434, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000076 DASUIV010000000
VERSION     DASUIV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111434"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFW59_02835"
     CDS             <1..350
                     /locus_tag="VFW59_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX5363179.1"
                     /translation="GPDVPLHFSAFHPDYKMMDTPPTPPETLGRARKIAMAAGLHYVY
                     TGNVHNREGDTTFCPSCKHAVIVRDWYDIEQYRLTPDGHCPDCGAQIAGHYETFTGAF
                     GQRRIPIDMRDMA"
     gene            complement(453..1223)
                     /gene="djlA"
                     /locus_tag="VFW59_02840"
     CDS             complement(453..1223)
                     /gene="djlA"
                     /locus_tag="VFW59_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HEX5363180.1"
                     /translation="MFKLIGIIAGYWLFGFIGAFLGFIGGSFIDRLRAYGSGGMNPLR
                     NAIRQAVFIETLFISMGKLAKVDGHVSQEEIEHVEQFMQKLGMTADHRQQAIVLFKKG
                     ADPEFDIEPTYKRFMSVCGQTRNLKEVLLAYLIVMALADGKFHPAEEALLTDIARHLG
                     FGEAEFKKMLEMVLNQSHFGGQANAAVALDDAYKALGVSKDSTDAEIKRAYRKLMSQY
                     HPDKLMGQGMPEDMIAMATEQAKEIQLAYDLVKKKREM"
     gene            complement(1771..>3268)
                     /locus_tag="VFW59_02845"
     CDS             complement(1771..>3268)
                     /locus_tag="VFW59_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5363181.1"
                     /translation="GYGAPARAAQLLNGLGFATDSLERPVTSFSGGWRMRLNLAQALM
                     CPSDLLLLDEPTNHLDLEAILWLEQYLSRYPGSILLVSHDREFLNATVNRIAHVHDCV
                     IDNYAGDYDDFERARAERIAQQNQAFQTQQAKIAHLEDFVRRFRAKATKAKQAQSRVK
                     ALERLTRIAPAHVADGHFELEIEAPERSPDLLMRADKMGFGYGDKQLFQNVDLLLRAG
                     ARIALLGPNGAGKSTLIKLLVGELAPTSGKLELTPEVRIGYFAQHQLENLDSNATPLQ
                     HMERVAPKETTLALRTFLGRFGLAGNEEDRPVATFSGGEKSRLALALLAWQRPHLLLL
                     DEPTNHLDLDMRDALTLALEEYTGAVVIVSHDRSLIRAVADELWLVADGKAQLFDGDL
                     EDYKSWIEARRPVETAQVQKVKPVAAAPKPNRKALLSKQGKLDLALSKAQSELGDINR
                     QLGDPDTYSSCSSEFIAELNAKRVVLEAKVAELEESWLELEMSLEAAV"
BASE COUNT          643 a         1043 c          941 g          641 t
ORIGIN      
        1 tgggaccgga tgtaccgctg cacttttccg cattccaccc cgactacaag atgatggaca
       61 ccccgcccac gcccccggag acgctgggcc gggcaaggaa gatcgcgatg gctgccgggc
      121 tgcactacgt ctacaccggc aacgtgcaca accgcgaggg cgacaccacc ttctgcccgt
      181 cctgcaagca cgccgtgatc gtgcgcgact ggtatgacat cgaacaatac cgcctcactc
      241 cggacggaca ctgcccggac tgcggcgcac agatcgccgg acactacgag acattcaccg
      301 gcgcattcgg tcagcgccgc atcccgatcg acatgcggga catggcctaa gcgactacac
      361 ctccccgcaa tagaaatggg ccaggtcgca atcgacccgg cccattaaat tttcagcgca
      421 tccgaagcga ttcgggctgc ggtctcgtcg acctacatct cccgcttctt cttgaccaaa
      481 tcgtaggcca gctggatttc cttggcctgt tcggtcgcca tggcgatcat gtcttccggc
      541 atcccctgcc ccatcagctt gtccggatga tattggctca tcagcttgcg ataggcgcgc
      601 ttgatctctg cgtcggtact atccttgctc acgccgagcg ccttgtatgc atcgtccagc
      661 gcaacggcag cgttagcctg cccaccgaag tgcgactggt tcaacaccat ctccagcatc
      721 ttcttgaact cggcctcacc aaagccgagg tgacgtgcaa tatcggtgag cagcgcctct
      781 tccgccggat ggaattttcc gtccgccagc gccatcacga tcagataggc cagcagcact
      841 tccttcaggt tacgggtctg accgcaaacg gacatgaaac gcttataggt cggctcgata
      901 tcgaactctg gatcggcgcc cttcttgaac aggacgatgg cctgctgacg atggtcggcg
      961 gtcatgccca gcttctgcat gaactgctcg acatgctcga tctcttcctg cgagacatga
     1021 ccgtcgacct tggccagttt ccccatggag atgaacaggg tttcgatgaa gaccgcctga
     1081 cgaatcgcat tcctgagcgg attcatcccg cccgaaccgt aggctcgcag acgatcgatg
     1141 aaagagccgc cgatgaagcc gaggaaggca ccgatgaaac cgaacagcca ataacccgcg
     1201 atgatgccga tgagcttgaa caagagaact ccagaataaa aaggcggcga ctgaccgcga
     1261 taaggacgcg aattatagcc acgaagattg gtacgcgcat cgccgaatcc aatcgccttc
     1321 tctgcggcgc gccttgggcc aactctggct atttcctgcg gccgggatct accccgtcga
     1381 ggtgtcgagc gcgcgggatg cgatcggtac tgggctacgc acttgaaata gccgaatgtt
     1441 tcactctgac atagcgcgtt tttgtgattt ttttcgattg gaaattatgc taaattttca
     1501 ccgggctgaa tttctcaccc gctgattaac caatctggag gagacctgaa tgaacatttt
     1561 aactaccata ctgaccgtta tcggaatcgc cgcagtgatt ggcatcatcg tggcgaccat
     1621 caccgaaaaa atgagaaaag actgaagtca gcgggcctgg ttcaaaccgg accaggcccg
     1681 cgctctactc cgatcaagac gacatcccgc aggacgggat cgaacaccag ccaactatct
     1741 gtcagataac cactcacaga ctgcgatgag ctacaccgca gcctccagac tcatctccag
     1801 ctccagccag ctctcttcca gttccgccac cttggcttcg agaacgaccc gtttggcatt
     1861 caactcggcg atgaattcgc tgctgcaact gctgtaggta tccggatcgc ccagctggcg
     1921 gttgatatcg cccagttcgg attgcgcctt gctcagcgcc aagtcgagct taccctgttt
     1981 ggacagcagc gccttgcggt tcggcttggg cgcggcagcg accggtttca ccttctgcac
     2041 ctgtgccgtc tccaccggac gacgcgcctc tatccagctc ttgtagtctt ccagatcgcc
     2101 gtcgaacagc tgagccttgc cgtccgccac cagccacaac tcgtcggcca ccgcacggat
     2161 cagactgcgg tcgtgcgaga cgatcaccac cgcaccggtg tattcctcca gcgccagcgt
     2221 cagcgcatcg cgcatgtcca gatcgaggtg gttggtcggc tcgtcgagca gcagcagatg
     2281 cggcctttgc caggccagca gcgccagcgc caggcggctc ttctcgccgc cggagaaggt
     2341 cgccaccgga cggtcctcct cgttgcccgc cagcccgaat cggccgagga aggtgcgcag
     2401 cgccagcgtg gtctccttgg gtgcgacccg ttccatatgc tgcaagggcg tggcgttgct
     2461 gtcgaggttc tccagctgat gctgggcgaa gtagccgatg cgcacctccg gggttagctc
     2521 cagcttgccc gaggtcgggg cgagttcgcc caccagcagc ttgatcagcg tggacttgcc
     2581 cgcaccgttc ggccccagca aggcgatgcg cgcaccggcg cgcagcagca ggtcgacatt
     2641 ctggaacagc tgcttgtcgc catagccgaa acccatcttg tccgcgcgca tcagcagatc
     2701 ggggctgcgc tccggcgctt cgatctccag ctcgaaatgg ccgtcggcca catgggccgg
     2761 ggcaatgcgg gtgagccgtt ccagcgcctt gacgcggctc tgcgcctgct tggccttggt
     2821 ggccttggcg cggaagcggc gcacgaaatc ttccagatgc gcgatcttcg cctgctgcgt
     2881 ctgaaacgcc tgattctgct gggcgatgcg ctcggcgcgg gcgcgctcga agtcgtcgta
     2941 gtcgcccgca taattgtcga tcacgcaatc gtgcacatgg gcgatgcggt tgacggtggc
     3001 gttgaggaat tcgcggtcat gcgagaccag cagaatgctg ccggggtagc gcgacagata
     3061 ttgttcgagc cagagtatcg cttccagatc gaggtggttg gtgggctcgt cgagcaggag
     3121 caggtcggac gggcacatca gggcctgggc caggttcagg cgcatgcgcc agccgccgga
     3181 gaagctggtc accggacgct ccagcgaatc ggtggcgaag cccagcccat tcagcagttg
     3241 cgcggcgcgc gctggggcac catagccg
//
LOCUS       DASUIV010000077         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_205077, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000077 DASUIV010000000
VERSION     DASUIV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205077"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="VFW59_02850"
     CDS             <1..1490
                     /locus_tag="VFW59_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5363182.1"
                     /translation="CRIAGLEVVRLVNEPTAASLAYGLDRLGQELRIAVIDLGGGTLD
                     VTIMEFGKGVFEVKATSGDTQLGGTDMNQRLFEHLAERFQMSTGVDVRQDQKAAARLI
                     EAAETAKIELSTSVTTHLSLPYLASVNGEPRHLELDLNRTELERLVRPVIERCRGPVE
                     QALHDAGITPKDVDRVVFVGGPTRMPVVRSYFEEMFGHKAEMGVDPMECVAAGAAVQA
                     GVLAGEVSGIVLVDVTPLTLGVETLGGIATPLIARNTPVPIKRSEIFTTAADMQTSVT
                     IHVFQGERPMAGDNTSLGEFNLDGLPPAPRGIPKIEVTFDIDSNGILSVSAKDTASGK
                     TQSISISGSTRLSEDTKKRMVEDAERYADADKKRREDAEKLNTADAGCYEAEKMLTNY
                     ADKLTDELKKRIETALRETKEALLKKDVTLATERAEALKKILQEAGAVLYAQSGQASK
                     GGPYAETRWEGPEPAPNVGAATGEPRASGSGPRGKVVDAEYKENR"
     gene            1574..1936
                     /locus_tag="VFW59_02855"
     CDS             1574..1936
                     /locus_tag="VFW59_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363183.1"
                     /translation="MMKRRLILSVLAAALAIPASFAIAADTAKTMTQEQSYGSQLMTP
                     EERAEHQARMRAAKTAEEREQIRKEHHERMTARAKERGVTLPDEPPARGGRMMPGGGM
                     GPGGGMGPDGGMSGGRNR"
BASE COUNT          480 a          674 c          694 g          327 t
ORIGIN      
        1 cctgccgcat cgccggcctt gaggtcgtgc ggctggtcaa cgaacccacc gccgcatcgc
       61 tggcctacgg cctcgaccgc ctcgggcagg agttgcgcat cgccgtgatc gacctgggcg
      121 gcggtacgct ggacgtgacc atcatggaat tcggcaaggg cgtgttcgag gtcaaggcga
      181 ccagcggcga cacccagctc ggcggcaccg atatgaacca gcgcctcttt gagcatctcg
      241 ccgaacgttt ccagatgtcc actggcgtgg atgtccgtca ggatcaaaag gcggccgcgc
      301 gactgatcga ggcggctgaa acggccaaga tcgaactctc caccagcgtc accacccacc
      361 tcagcctgcc ctatcttgcc tccgtcaacg gcgagccccg ccatctggaa ctggacctca
      421 accgcaccga acttgagcga ctggtgcgcc cggtgatcga acgctgccgt ggcccggtcg
      481 agcaggcgct gcacgacgcg ggcatcacgc ccaaggatgt ggatagagtg gtgttcgtcg
      541 gcggcccgac gcgcatgccg gtggtgcgca gctacttcga ggagatgttc gggcacaagg
      601 ccgagatggg agtggacccg atggaatgcg tggctgccgg agccgcagtc caggccgggg
      661 tgctggcagg cgaagtcagc ggcatcgtgc tggtagatgt gacgccgctg accctcggcg
      721 tcgagacgct gggcggcatc gcaacgccct tgatcgcgcg caacacaccg gtgccgatca
      781 agcgcagcga gatcttcacg accgccgccg acatgcagac ttccgtcacc atccatgtct
      841 tccagggcga gcgcccgatg gcgggcgaca acaccagcct gggcgaattc aatctcgacg
      901 gcctgccgcc cgccccgcgc ggtatcccca aaatcgaagt gaccttcgac atcgattcca
      961 acggcatcct gagcgtatcg gccaaggaca ccgccagcgg caagacccag tccatcagca
     1021 tcagcggttc cacaagactg tcggaagaca ccaagaaacg catggtggag gacgccgagc
     1081 gttatgccga tgcggacaag aagcggcgtg aggatgcgga gaaactcaat accgccgatg
     1141 ctggctgcta tgaggcggaa aagatgctga ccaactacgc cgacaagctc accgacgagt
     1201 tgaaaaaacg catcgagact gcattgcgcg agaccaaaga agcgctgctg aagaaggatg
     1261 tcacgctcgc caccgagcga gcagaggcgc tgaagaaaat cctgcaggag gccggtgcgg
     1321 ttctctatgc ccagtccggt caggcatcga aaggcggccc ctatgcggaa acacgctggg
     1381 aaggccccga acctgcaccc aatgtgggcg ctgcgaccgg cgagccgcga gcaagcggct
     1441 ccggccctcg cggcaaggtc gtcgatgccg agtacaagga aaaccgctga tttgccatca
     1501 cctaaaccgc tgctgaagag tggtcgtggc gggaaccaca ggtcatacaa cctgcattga
     1561 caaggagtcc gagatgatga aacgtcgatt gattttatcc gttctggctg ctgcactggc
     1621 catccctgcc agcttcgcga ttgccgccga cactgccaaa accatgactc aggagcagag
     1681 ctacggcagc cagttgatga cgccagagga acgcgccgaa caccaggcca ggatgcgtgc
     1741 ggcgaaaact gccgaggagc gggagcaaat ccgcaaggag catcacgagc gcatgacggc
     1801 gcgcgcgaag gagcgtggcg ttacccttcc cgatgagcct ccggccagag gcggcagaat
     1861 gatgcccggc ggcgggatgg ggccgggtgg tgggatgggg ccggatggcg gcatgagtgg
     1921 ggggcgtaat cgctgagcga tgtctgacag cctggatgga tgctcccgct cggagcatcc
     1981 gaaaagggat cacgaagaaa aacccgaaaa tactctgtac cggccatcat gaccccacct
     2041 cttccgctga aagcgcacaa actgcaccaa cccagtgatc cgaagcaatt cggcttccag
     2101 actaccgacg agctggagga tctgggcgag atcatcggcc agatgcgtgc gatggaggcg
     2161 atacgcttcg gcacc
//
LOCUS       DASUIV010000078         9553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1041118, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000078 DASUIV010000000
VERSION     DASUIV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041118"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFW59_02860"
     CDS             complement(<1..494)
                     /locus_tag="VFW59_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5363184.1"
                     /translation="MLHLTLRQLQVFEKVASHLSYSRAAEALYLSQPAVSMQIKQLEE
                     NIGLPLFEQMGKKIYLTEAGREVLHYSRNILHQLNEMESVFADMKGLGQGTLILSVVN
                     TANYFTPQLLADFCRRYPEINVTLQVANRDAVLQQLADNHTDLAIMGQPPEGLDLVAE
                     SFMD"
     gene            651..2030
                     /locus_tag="VFW59_02865"
     CDS             651..2030
                     /locus_tag="VFW59_02865"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HEX5363185.1"
                     /translation="MDQSNRYSDLSLKEEDLIKGGNHVLTAYIMKPKAGHGFLEVAAH
                     VAAESSTGTNVEVSTTDAFTKGVDALVYEINEETGLVKIAYPVDLFDINIIDGRAMIA
                     SFLTLTIGNNQGMGDCEYLKMHDFYMPPKYLRLFDGPNVNIVDMWRSLGRPVENGGYI
                     AGTIIKPKLGLRPEPFAKAAYQFWLGGDFIKNDEPQGNQPFCPLKKVIPLVVDAMNRA
                     QDETGEAKMFSANITADDPAEMIARAEFILDAFGFNAPRVAFLVDGYVAGPTAVTVAR
                     RNFPAQFLHFHRAGHGAVTSPQSKRGYTAFVHCKMTRLMGASGMHVGTMGYGKMEGES
                     SDKLISFMLDDDVAQGPYFKQEWNGMKATSSIISGGMNALRLPGFFANLGHGNVINTA
                     GGGAYGHIDSPAAGAKSLRQAYECWKAGADPIEYAKEHKEFARAFESFPKDADKLFPG
                     WREKLGVHK"
     gene            2206..3009
                     /locus_tag="VFW59_02870"
     CDS             2206..3009
                     /locus_tag="VFW59_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEX5363186.1"
                     /translation="MSNIDQYKVHKEPFYQAQSDEVVLYAAAYAARLPVMVKGPTGCG
                     KSRFVEYMAWKLNKPLITVACNEDMTASDLVGRYLLDANGTRWLDGPLTTAARIGAIC
                     YLDEIVEARQDTTVVIHPLTDHRRTLPLDKKGELIEAHPDFQLVISYNPGYQSLMKDL
                     KQSTKQRFVGFDFDYPAASVEAEILGKETGIEAGLAAQLVKIGHAARNLKGHGLDEGI
                     STRLLVYAATLIKGGVEPKAACRMALVRPITDDADIRDTLDHAIEATFA"
     gene            3014..5359
                     /locus_tag="VFW59_02875"
     CDS             3014..5359
                     /locus_tag="VFW59_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5363187.1"
                     /translation="MTTETVEIPEDLQAYWAQLSCGFARVGLIFPDCMREALAVLSKD
                     GVNAYIENARFLGKMGRGAEPILIFLEEWPSVAKVLGEEVLPAVMDFVRKMTRSPNSK
                     AIAPFLQSLPTAARRLQSAQQFADYLTILEDCMERTTGSVHGIHQTFPSPGLPELFKQ
                     MPVLLSQLGLNGLKNWVDYGVRYYHDHPERQVEYFSLQSADSRAVLQRERMGTLLVDN
                     ERKLNMYLRCLWQDDDHLVPYSEVFDELRKPMPYFDSLGIRLPDAYSDYVLPSPASGQ
                     VAGYLPYDGRESNRLPLEEIVISGIDRYRAALAHIAAHRRWTRPVVADNISPFQRMAV
                     EFFEDTRVEYLAMKEYPGLRHLFMALHPVPIEDACDPDKQSAVRHRLAMVSRAMLDAS
                     CAYRNSDVCEIVERFRNIMAEGDSSTSEMVDLAISFIARTRRQADLSADVYFDNTEVS
                     YRDDNRHMWVFIEEGDEEDAFDKPEAPPQEELKGLPPRHYPEWDYNSQSYRPDWVSVY
                     EGLHPSGNAADIDRLLDKHAGLAKRLKKLLDLLKPQDKVRIRYQEEGSELDLDVAIRS
                     LIDYKSGAQPDPRINMSHRNDGRNIAVMVLLDLSESLNEKAAGSGQTILELSQEAVSL
                     LGWAVEKLGDPFAIAGFHSNTRHDVRYMHIKGYSEKWGDEVKGRLAGMAASYSTRMGA
                     AMRHAAHYLGQQQADKKLMLILTDGEPADIDSKDGRILIEDARQAVKELDRDGIYAYC
                     INLDPKADEYVSDIFGKQYTIVDKVEQLPAKLPELFISLTK"
     gene            5428..6429
                     /locus_tag="VFW59_02880"
     CDS             5428..6429
                     /locus_tag="VFW59_02880"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX5363188.1"
                     /translation="MSKSLIQFIIEEQRAVPGASGDFTGLISDIVSACKQIAHDVNKG
                     ALIGVLGSAGSENVQGETQKKLDVITNEIFIKSNEWAGHLAAMASEEMDDIYPIPTKY
                     PKGKYLLTFDPLDGSSNIDVNISVGTIFSILRCPEGVTNPTAEDFLQPGTKQVCAGYA
                     LYGPNTMLVITTGNGVNGFTLDRDVGDFFLTHPGMSIPADSLEFAINASNERFWEAPV
                     QRYVKECIAGKTGPRGVNFNMRWVASMVAEVHRILTRGGVFMYPKDTKDPTKAGKLRL
                     LYEANPMSFIVEQAGGVSSTGYERIMELKPNGLHQRVPVILGSKNEVERLISYHKEA"
     gene            6548..7039
                     /gene="purE"
                     /locus_tag="VFW59_02885"
     CDS             6548..7039
                     /gene="purE"
                     /locus_tag="VFW59_02885"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5363189.1"
                     /translation="MSKPLVSIVMGSANDWEIMQQAARALQDFGVAFDARVISAHRSP
                     DLLFDYIKEMSDQGVQCFIAGAGGAAHLAGVIAGKTTLPVLGVPIPSKYLKGMDSLLS
                     IVQMPKGIPVATFAIGEAGSANAGLFAISMLALNDAVLAQKLTDYRKKQAEQIAATTL
                     PAL"
     gene            7054..7938
                     /locus_tag="VFW59_02890"
     CDS             7054..7938
                     /locus_tag="VFW59_02890"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5363190.1"
                     /translation="MAALHESNLTSLPLLHRGKVRDLYEVDAEHLLIVQTDRLSAFDV
                     ILPDPIPGKGAVLTAVSNFWFDKLKEVIPNHLSGIDPESVVKTEEERAQVRGRAFVTK
                     KLKPLPIEAIVRGYLVGSGWKDYQKTGSVCGIALPAGLREAQKLPQPLFTPSTKAAVG
                     DHDENISFVQAVALLGEARAHEVRDATLALYSQAADYAATRGIIIADTKFEFGVDDAG
                     KLYLIDEALTPDSSRFWPADQYQVGSNPPSFDKQFVRDWLETSGWNKQAPAPRIPQDV
                     LQKTADKYREAQQRLIGT"
     gene            7935..8570
                     /locus_tag="VFW59_02895"
     CDS             7935..8570
                     /locus_tag="VFW59_02895"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5363191.1"
                     /translation="MTSPHDLIEGFHRFREQHFADDDALYRQLIQDGQTPKILVVACC
                     DSRVDPALVLNCAPGDLFVIRNVANLVPPAESRSGGRHGTSAALEYGVHTLGVEHIIV
                     LGHAHCGGIRSLVQTGGANNPASFIDDWMRLVEDARVSVERDMPGESLEKRTQACEQR
                     AILGSLDNLMTFSWVCERVEQGTLTLHGWYFDMENGRLLGYNPATRLFETL"
     gene            complement(8571..9179)
                     /locus_tag="VFW59_02900"
     CDS             complement(8571..9179)
                     /locus_tag="VFW59_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5363192.1"
                     /translation="MHNSKLIIALIAGLAFSLPVNAKMYKWVDDKGVTHVGDTIPPEY
                     ADKDRTELNKSGRAIKKDEVLNAEERRAKDQLDNQKRAADEAVLESRRHDKALLSTYS
                     NSAEIDLARSRNMQQIDARVSGMASQVSIVNDRLLGLQKEAEGRTKAGKKIPQSLQED
                     LQETQERLRKLKEDHEKAKADKAAMEARYDADKARYKELTGK"
     gene            complement(9172..>9553)
                     /locus_tag="VFW59_02905"
     CDS             complement(9172..>9553)
                     /locus_tag="VFW59_02905"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5363193.1"
                     /translation="RNVPYCFNRKEAKDHGEGGTMVGAKLQGRVLIIDDVISAGTSVR
                     ESIELIRAAGAEPCGVVIALDRMERGQGELSAVQEVQRNYGIPVVHIAGVDDLLSYLQ
                     GEPEMVQNLSAVKSYRAQYGVKNA"
BASE COUNT         1959 a         2660 c         2868 g         2066 t
ORIGIN      
        1 ttgtccatga acgattcggc gacaagatcg agaccctctg gaggctgacc catgatggcc
       61 agatcggtgt ggttgtcggc cagttgttgc agcacggcgt cacggttggc aacttgcagt
      121 gtgacgttga tctctggata gcgccgacag aagtccgcga gcagttgcgg ggtgaagtag
      181 ttggcggtgt tgaccaccga caggatcagc gtgccctgcc ctagcccctt catgtcggca
      241 aacaccgact ccatctcgtt gagttggtgc aggatattgc ggctgtagtg cagcacttcg
      301 cgtccggcct cggtcaggta aatcttttta cccatctgtt cgaacaaggg caggccgatg
      361 ttctcttcca gttgcttgat ctgcatggac accgcaggct gcgaaagata tagtgcttcg
      421 gcagcgcgcg agtagctcag gtggctggcc accttttcga acacttgtaa ttgacgcagc
      481 gtgagatgga gcatgggcgg acgaaatatc agggtgttgc gattataaca aaccataagc
      541 gccccttatg gtttgaataa aaataattga ctatctctta tgtgacgccg tctctatagt
      601 caccaccgtt gcagataaaa accacttcta aacacactgg agagaaattc atggatcaat
      661 ccaatcgtta ttccgatctg agcctgaaag aggaagatct gatcaagggc ggtaaccacg
      721 ttcttaccgc ttacatcatg aagccgaaag ctggtcatgg cttcctggaa gtggctgcgc
      781 acgtcgctgc cgagtcttcc accggcacca acgttgaagt ttccactacc gatgcattca
      841 ccaagggtgt agatgcgctg gtatacgaaa tcaacgaaga gaccggtctg gtgaagatcg
      901 cttacccggt tgacctgttc gatatcaaca tcatcgacgg ccgcgcgatg attgcctcgt
      961 tcctgacgct gaccatcggt aacaaccagg gtatgggcga ttgcgaatac ctgaagatgc
     1021 atgacttcta catgccgcct aagtacctgc gcctgtttga tggtcctaac gtcaacatcg
     1081 tcgatatgtg gcgctcgctg ggtcgtccgg ttgaaaacgg cggctacatc gccggcacca
     1141 tcatcaagcc taagctgggc ctgcgtccag agccgtttgc gaaggctgct taccagttct
     1201 ggctgggcgg cgacttcatc aagaacgacg agcctcaagg caaccagccg ttctgcccgc
     1261 tgaagaaggt gatcccgctg gtcgtggacg cgatgaaccg cgctcaggac gagactggcg
     1321 aagcgaagat gttctccgcc aacatcactg ccgatgatcc tgctgaaatg atcgcccgcg
     1381 ccgagttcat cctcgatgcg ttcggcttca acgcaccgcg cgtcgccttc ctggtggacg
     1441 gctatgtggc tggcccaacc gctgtgactg ttgctcgccg caatttcccg gcacagttcc
     1501 tgcacttcca ccgcgcaggt cacggtgcag tgacttcgcc gcagtccaag cgtggctaca
     1561 ccgcattcgt acactgcaag atgactcgtc tgatgggtgc gtccggtatg cacgtcggca
     1621 ccatgggtta cggcaagatg gaaggcgagt cttccgacaa gctgatctcc ttcatgctgg
     1681 atgacgatgt cgcacagggt ccatacttca agcaggagtg gaatggtatg aaggctacca
     1741 gctccatcat ctccggcggc atgaacgcgc tgcgtctgcc aggcttcttc gcgaacctcg
     1801 gccacggcaa cgtgatcaac accgcaggcg gcggcgctta cggtcacatc gacagcccgg
     1861 ctgcaggtgc gaagtcgttg cgtcaggcgt acgagtgctg gaaagccggt gccgatccga
     1921 tcgaatacgc aaaggagcac aaggaatttg cccgcgcatt cgaatccttc ccgaaggatg
     1981 cggacaagct gttcccgggc tggcgcgaaa agctgggcgt acacaagtaa gctcggcggt
     2041 agctacgcaa cgaaaaggcg cccccactcg ttgggggcgt ttttttgact ccggccgacc
     2101 gcaagcggcc ttaaaatgcc aaggcatgtt caccgggtac aagagcctgc gtcgcaacaa
     2161 gccgttgtgc ggcgtgttgt ccgcgaatac agagaggtta tgaagatgag caatattgac
     2221 caatacaagg tacacaagga accgttctat caggcgcagt ccgatgaggt cgtgctgtat
     2281 gcggcggcct atgccgcccg cctgccggtg atggtgaagg gtccaaccgg ctgcggcaag
     2341 tcgcgcttcg tcgaatatat ggcgtggaaa ctcaacaagc cgctgatcac cgtggcttgt
     2401 aacgaggaca tgactgcatc cgatctggtc ggacgttatc tgcttgacgc caacggcaca
     2461 cgctggctcg acggcccgct gaccacggcg gcgcgtatcg gggcgatctg ctatctggac
     2521 gagatcgtcg aggcgcgtca ggacaccacc gtggtgatcc acccgctgac cgaccatcgc
     2581 cgcaccttgc cgttggacaa gaagggcgag ctgatcgagg cgcatccgga cttccagctg
     2641 gtgatttcat acaaccccgg ttaccagagc ctgatgaagg atctgaagca gtcgaccaag
     2701 cagcgcttcg tcgggttcga tttcgactac ccggcggcaa gcgtcgaggc ggaaattctg
     2761 ggcaaggaga ccggcatcga ggccgggttg gcggcgcagc tggtgaagat cggtcatgcg
     2821 gcgcgcaatc tcaaggggca tggcctggat gaaggtatct cgacccgttt gctggtatat
     2881 gccgcgaccc tgatcaaggg tggcgtcgag ccgaaggcgg cttgccggat ggcattggtg
     2941 cgtccaatca ccgacgatgc cgacatccgc gacacgttgg atcacgccat cgaggcgacc
     3001 ttcgcctagc gccatgacca ccgagacggt cgaaatcccg gaagacttgc aagcttactg
     3061 ggcgcagttg agctgcggat ttgcgcgcgt cggtctgata ttcccggact gcatgcgcga
     3121 ggcgctggcg gtgctgtcga aggacggcgt gaatgcctat atcgagaatg cgcgtttcct
     3181 cggcaagatg gggcgcggcg cggagcccat tctgatcttc ctggaggaat ggccctcggt
     3241 cgccaaggtg ttgggcgagg aagtgttgcc tgcagtgatg gatttcgtgc gcaagatgac
     3301 ccggtcgccc aacagcaagg ccatcgcgcc gttcctgcag tcgctgccga cggctgcgcg
     3361 gcgtttgcaa tcggcgcagc agttcgccga ttacctgacc attctggagg attgcatgga
     3421 gcgcaccacc ggctcggtgc atggcatcca ccagaccttc cccagtcccg gcctgccgga
     3481 gttgttcaag cagatgccgg tgctgctcag ccagctcggg ctgaatgggc tgaaaaactg
     3541 ggtcgattac ggcgtgcgct attaccacga ccatcccgag cggcaggtcg aatacttcag
     3601 tctgcagtcg gccgacagtc gtgcggtgct gcagcgcgaa cgcatgggca cgctgctggt
     3661 ggataacgag cgcaagctga atatgtatct gcgctgcctg tggcaggacg acgatcatct
     3721 ggtgccctat tccgaggtgt tcgacgagtt gcgcaagccg atgccctatt tcgactcgct
     3781 cggcatccgt ctgccggacg cctatagcga ttatgttctc ccctcccccg caagcgggca
     3841 agtcgctggt tatctcccat atgatgggag agagagtaat cgtcttcctt tggaagaaat
     3901 tgttatttct ggaatagatc gctaccgcgc cgcgctggcg catatcgccg cgcatcgccg
     3961 ctggacccgg ccggtggtcg ccgacaacat cagcccgttc cagcgcatgg cggtggagtt
     4021 tttcgaggac acccgcgtcg aatatctggc gatgaaggaa tatccgggtc tgcgccatct
     4081 gttcatggcg ctgcatccgg tgccgataga ggatgcctgc gatccggaca agcagtcggc
     4141 ggtgcgccat cgtctggcga tggtgtcgcg cgcgatgctc gatgcttcat gcgcttaccg
     4201 caattccgac gtgtgcgaga tcgtcgagcg tttccgcaac atcatggccg aaggcgattc
     4261 gtcgacctcc gagatggtgg atctggcgat ctcgttcatc gcgcgcactc gccgccaggc
     4321 cgatctgtcg gccgacgtgt atttcgacaa caccgaggtc agctaccgcg acgacaaccg
     4381 ccacatgtgg gtgttcatcg aggaaggcga cgaggaggat gccttcgaca agccggaagc
     4441 gccgccgcag gaagagctca agggcctgcc gccgcgacat tatccggaat gggactacaa
     4501 cagccaaagc taccgcccgg attgggtcag cgtgtatgag ggcttgcatc cttccggcaa
     4561 cgcggccgat atcgatcgtt tgttggataa acatgccggt ctggccaagc gcctgaagaa
     4621 gctgctcgat ctgctcaagc cgcaggacaa ggtgcgcatc cgctatcagg aagaaggcag
     4681 cgagctggat ctggacgtcg cgatccgttc gctgatcgac tacaagagcg gcgcacaacc
     4741 cgatccgcgc atcaacatga gccatcgtaa cgatgggcgc aacatcgcgg tgatggtgct
     4801 gctggatttg tccgagtcgc ttaacgaaaa agcggcaggc agcggtcaga ctattttgga
     4861 gttaagccag gaggcggtgt cgctgttggg ctgggcggtg gaaaagctgg gcgatccttt
     4921 cgccatcgcc gggttccatt ccaacacccg tcacgatgtg cgttacatgc atatcaaggg
     4981 ctacagcgaa aaatggggtg atgaggtgaa ggggcggctg gcagggatgg cggctagtta
     5041 ttccacgcgc atgggggcgg cgatgcggca tgccgcccac tatctggggc aacagcaggc
     5101 agacaagaag ctgatgctga ttctgaccga cggcgagcct gccgacatcg acagcaaaga
     5161 tggacggatc ctgattgagg atgcgcgtca ggcagtaaaa gaactggatc gggatggcat
     5221 ctacgcctat tgcatcaatc tcgatcccaa ggcggatgaa tatgtgtcgg acatctttgg
     5281 caagcaatac acgatcgtcg acaaggtgga gcaactgccc gcgaaattgc ccgaattgtt
     5341 catttcgctg accaaatagg cgtagagagc cgggcggaaa acctgtaaaa tccggcggtt
     5401 gataattttt aatgtgagga gagcagcatg agcaagagtt tgattcagtt cattatcgaa
     5461 gaacaacgcg cggttcctgg cgcttccggt gatttcaccg gtctgatcag cgacatcgtt
     5521 tctgcttgca agcagatcgc tcatgacgtg aacaagggtg cgctgattgg cgtgctgggc
     5581 agcgccggca gcgaaaacgt gcagggtgag acccagaaga aactggacgt catcaccaac
     5641 gagattttca tcaagtccaa tgagtgggcc ggtcatctgg ctgcgatggc ttccgaagag
     5701 atggatgata tctatccgat cccaaccaag tacccaaagg gcaagtacct gttgaccttc
     5761 gatccgctgg atggctcttc caacatcgac gtgaacatct ccgttggcac catcttctcg
     5821 atcctgcgct gccctgaagg cgtgaccaac ccgaccgcag aagactttct gcagccaggc
     5881 accaagcagg tctgcgccgg ttatgcgctg tacggcccga ataccatgct ggtgattacg
     5941 accggtaatg gcgtgaacgg ctttacgctg gatcgcgatg ttggtgactt cttcctgact
     6001 cacccaggca tgagcattcc tgccgattcg ctggaatttg cgatcaacgc atccaacgag
     6061 cgcttctggg aagcgccggt gcagcgttac gttaaagaat gcatcgccgg caagacaggc
     6121 ccgcgcggcg taaatttcaa catgcgttgg gttgcctcga tggtggcgga agtgcatcgt
     6181 atcctgaccc gtggcggcgt gttcatgtac cccaaggaca ccaaagaccc aaccaaggcc
     6241 ggcaagctgc gtctgttgta cgaagctaac ccgatgtcct tcatcgtcga gcaggcgggc
     6301 ggtgtatcct ccaccggtta cgagcgcatc atggaactca agccgaacgg tttgcatcag
     6361 cgcgtgccag tgattctcgg ttcgaagaac gaagtcgagc gactgatcag ctatcacaag
     6421 gaagcgtaat aactgcaacg cccccgtgcg cgggggcgtt gtttcgtccg caggaattaa
     6481 ctattgctct tctcgtttgt ctcctctccc gcttgcggga gaggattgag gagagggaag
     6541 gaatatcatg agcaaaccac ttgtttccat cgtgatgggt agtgccaacg actgggagat
     6601 catgcaacag gcggcgcgtg ccctgcagga ttttggtgtg gctttcgatg cgcgtgtgat
     6661 ttcggcgcac cgctcgcccg atctgctgtt tgactacatc aaggaaatgt ccgatcaagg
     6721 cgtgcaatgc ttcatcgcgg gcgcaggcgg cgcggctcat ctggctggtg tgatagcagg
     6781 caagacgacg ttgccggtat tgggcgtgcc tatcccctcg aagtatctga agggaatgga
     6841 ttcgctgctg tctatcgtgc agatgcccaa gggtattccc gttgcgactt tcgccattgg
     6901 tgaagccggt tcggccaatg ccgggctgtt tgccatatcc atgttggcgc tgaacgatgc
     6961 cgtcttggca cagaagctga ccgactatcg caagaagcag gcggagcaga tcgccgcgac
     7021 caccttgccc gccctgtaat agagaggttc ccgatggccg cattacatga atccaacctg
     7081 accagcctgc cgctgttgca ccgaggcaag gtgcgcgacc tttacgaagt ggacgcagaa
     7141 catctgctga tcgtgcagac cgatcgtttg tcggcgtttg acgtgatcct gcccgatccc
     7201 attccgggca agggcgcggt gctgactgcc gtgtcgaact tctggttcga caagctgaaa
     7261 gaggttattc ccaatcatct ttccggaatc gatccggaat cggtggtcaa gacggaggaa
     7321 gagcgtgctc aggtgcgcgg tcgagccttt gtgaccaaga agcttaagcc gttgccgatc
     7381 gaggccatcg tgcgcggtta tctggtgggt tccggctgga aggactacca gaagaccggg
     7441 tcagtatgcg gcattgcatt gccagccggt ttgcgggaag cgcaaaagct gccgcagccg
     7501 ttgttcaccc cgtctaccaa ggcggcggtg ggtgatcatg acgagaacat ctcgtttgtg
     7561 caggcagtgg cattgctcgg cgaggcgcgc gcccatgagg tgcgtgacgc gactttggct
     7621 ttgtatagcc aggcggcgga ctatgcggcg accagaggca tcatcatcgc ggataccaag
     7681 ttcgagtttg gtgtggatga tgcaggcaag ttgtatctga tcgacgaggc attgacgccc
     7741 gattcatcgc gcttctggcc tgccgatcaa tatcaggttg gcagcaaccc gcccagtttc
     7801 gacaagcaat tcgtgcgcga ttggctggag acctcgggct ggaataaaca ggcacccgct
     7861 ccgcgtattc ctcaggacgt gttgcagaaa accgccgaca agtaccgtga agcacagcaa
     7921 cgtttgatcg gaacatgacc agcccgcacg acctgatcga gggttttcat cgttttcgcg
     7981 agcagcattt cgcggatgac gatgcgttgt atcgtcagtt gattcaggac gggcagacgc
     8041 ccaagatact ggtggtcgcc tgttgcgatt ctcgtgtaga tccggcgctg gtgctgaact
     8101 gcgcgcccgg tgatttgttc gtgatccgca acgtggcgaa tctggtgcct cctgccgaga
     8161 gtcgcagcgg cggtcggcac ggcactagcg cggcgctgga atatggtgtg cacaccctgg
     8221 gtgtcgagca catcatcgtg ctgggacatg cgcattgcgg cggcatccgc tcgctggtgc
     8281 agaccggcgg ggccaacaat ccggcctcgt tcatcgatga ctggatgcgt ctggtcgaag
     8341 atgcgcgagt gagcgtggag cgcgacatgc caggcgagtc gctggaaaag cggacgcagg
     8401 cctgcgagca gcgcgcgatt ctgggttcgc tcgacaacct gatgaccttt tcctgggtat
     8461 gcgagcgggt cgagcaaggt acgctaacct tgcacggctg gtacttcgat atggagaacg
     8521 ggcggctgct gggttacaac ccggccaccc gtttgttcga gacgctgtaa ttacttgccg
     8581 gtcagttcct tgtagcgcgc tttgtcggca tcgtaacgcg cttccattgc cgccttgtcg
     8641 gctttggctt tttcgtgatc ttctttcagt tttctcaggc gttcttgtgt ttcctgcagg
     8701 tcttcctgca acgattgagg aatcttttta ccggccttgg tacggccttc ggcttccttc
     8761 tgcagaccca gcagccggtc attgacgatg ctcacctgag aggccatgcc gctgacgcgc
     8821 gcatcgatct gttgcatgtt gcggctgcgc gccaagtcga tctcggccga attgctgtat
     8881 gtgctcagca gcgccttgtc gtggcgcctt gactccaata cggcttcatc ggctgcccgt
     8941 ttttgattgt cgagctgatc ctttgctctg cgttcttcgg cgttgagcac ctcgtccttc
     9001 ttgatggcgc gacctgactt gttcaattcg gtgcgatcct tgtcggcata ttccggcgga
     9061 atcgtgtctc ccacatgggt gacgcccttg tcgtctaccc atttatacat cttggcgttg
     9121 actggcaggc tgaatgccaa cccggcaatg agagcgataa tcaatttcga attatgcatt
     9181 tttgactcca tattgggcac ggtaagactt cacggccgac aaattctgca ccatctcggg
     9241 ctctccttgc aaataactga gcaggtcatc cacgccggca atgtgcacta ccgggatgcc
     9301 gtagttgcgc tgaacttctt gcaccgcaga aagctcgcct tgaccgcgct ccatgcgatc
     9361 cagtgcgatg accacgccac agggctcggc acctgctgcg cgaatcaatt cgatggattc
     9421 tcgtaccgag gttcccgccg agatcacgtc gtcgatgatc agcacgcggc cctgcaattt
     9481 cgcccctacc atcgtgccgc cttcgccgtg atccttggct tctttgcggt taaagcaata
     9541 aggtacattg cgt
//
LOCUS       DASUIV010000079         6994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_391013, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000079 DASUIV010000000
VERSION     DASUIV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391013"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VFW59_02910"
     CDS             <1..1283
                     /locus_tag="VFW59_02910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5363194.1"
                     /translation="NYANQPYDLVLLERARAVAEQMKLGSGQENLDFKPNLPNGEMLG
                     MPDRVFYTVSDKDGKKIVGNANLSRPLAYRGKKPGPVFSNSEHDGEKTRMVSLVYPGP
                     PGAKLYQLHMSETIHQRQALIRGILANIVIPQLLLLLIAGGALWYALKHGLRPLERLR
                     QDVARRQRDDLSRLDETRAPAEVRPLIAAVNDLLERLKQVMQSQKRFVADAAHQLRTP
                     FAGLKTQAELALRENEIERKQQALEHILTSARQSTHLVNQLLALARNEPGGQGADSFA
                     PLDLNHLAQECTVRWVALALEKNIDLGFEGIPVQAPVRGDADSLTEMLGNLIDNAIRY
                     TPEGGHITVSVDYQRGGAILQVEDNGPGIPPQHRERVFERFYRILGSGQSGSGLGLAI
                     VAEVAKRHGATIALGEGKNGAGTLISIRFPHHGS"
     gene            complement(1296..3716)
                     /locus_tag="VFW59_02915"
     CDS             complement(1296..3716)
                     /locus_tag="VFW59_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5363195.1"
                     /translation="MQQLVDVLPIAIFIKDADSKFLLMNSACEEQWGMRFSDLRGTDA
                     SQFFPPDQMEWFLAKDREIFAGGSQVDFEETFWNATLKQNRIGHTFKKPVYDSSGKPL
                     YLICITIDVTDARKIDQDLRLSEEKLRAMFDMSPLGMIRNSMDGTFVEANNALLNIVG
                     YSLEELNRLSYWDLTPESYSEQEAEQLESLRVHARYGPYEKEYINSSGQRVPVRLNGV
                     QITGSDGEKFIWSIVEDVTERRRNEEQLRSSNQRFEAVLQAVPDLMFELDRNGRYLNV
                     WGIRGNLLAAPENQLIGRTVEEMLPLIAAQEVLAAISEADEKGLSHGRSIRLQLPEGD
                     CWFELSVAKRPADSLQDAGFIVLSRDITERKSAEQQIYQLAFFDPLTHLPNRRLLMDR
                     LHQAFSVSARNGQHGAIMFLDLDNFKMLNDSRGHEFGDLLLIEVSRRLASCVREGDTV
                     ARLGGDEFVVVLETLSINMDEAATQAEAVAEKIQAALNQPYQIKEYSHHSTSSIGIVL
                     FRGHQENLDNLLKHADTAMYQAKTAGRNAIRFFDPDMQAVLEARAELESELRQALEKQ
                     QFRLHYQIQVDSKHRSLGAEALIRWEHPERGLISPAQFIPLAEESGIIEAIGLWVLGT
                     AAAQLAAWRQNPLTRNLTLAVNVSARQFRQPGFLAQVRQVLLESGANPFQLKLELTES
                     AVLERIEESISRMRELRALGIRFSVDDFGTGYSSLAYLTQLPIDQLKIDQSFVRNIGV
                     KPGDAVIAQTIIGMANNLGMEVIAEGVETEAQRSFLEQHGCSAYQGYLFSKPVPLAEF
                     EKQLSSYN"
     gene            3987..4979
                     /locus_tag="VFW59_02920"
     CDS             3987..4979
                     /locus_tag="VFW59_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5363196.1"
                     /translation="MKKLSRRQFIASSAGLALALAIPGCGWLADKPIAIAAHVWPGYE
                     PMFLARSEGWLDAEQVRLVETTSATGSLQALADGKVDGAALTLDEVLRGRAMGLPLSV
                     VMVFDVSAGADVLIARPGIKKLADLKGRRIGYEPGALGALMLAEALRAAHLTQNDVKP
                     VPFTIDKQHEAWMNDQVDALITYEPVSSQLLSQGAVTLFDSRRIPNTIIDVLAVRGDI
                     LDQRHAAAIRHLLSAHFRALDHLNRNPQDAAFRMASHMNLPASDVLPAFKGLVLPDAD
                     NNRRLLTGAAPELRETASKVSHVMVETKLLPRDDDMKGLIHAEFLPSPTAESHP"
     gene            4976..>6994
                     /locus_tag="VFW59_02925"
     CDS             4976..>6994
                     /locus_tag="VFW59_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5363197.1"
                     /translation="MNLPGSSRPESIRGLAVILALLAVLMSAALPVSPAHAAEPVKIG
                     VLAFRPKPQTLAQWQPLAQILKKAIPERDFVIEALTNQEMDMAVASRQLDFVLTNPSH
                     YVLLSHHSGLSSPLATLAEDARGQAVSVFGGVIFSRADSAGINSLADLKGKSIAALGT
                     ESMGGYQMQAYELKRQGIRPSQEIRLLSVGAPQDNVVNEVLAGHAEAGFVRTGVLEAL
                     SREGKLDMAQLKIIAPQNLPGFPAQVSTRLYPEWPFAALPHIDDNLARRVAAALLVLE
                     QNSAASKSLGIHGFVIPADYTPVEDMMRELRMPPFDLAPEFTLNDVWNQYRWQVIAAL
                     AACGLIAFLLARLLWTNRRLDAEHRTVLEQKQQLQESEYRWKFALEGSGDGLWDWNLT
                     DNTVFYSRRWKEMLGYREDEIGDSLGEWDKRVHPDDKTATLQTLQAHLDGKSPMYVSE
                     HRVHSKDGDWKWILDRGVVVSRDKDGKPLRMIGTHTDVTDRHQMEEQVRQLALYDSLT
                     KLPNRRLLNERLSHVMAASKRSGCYCALMFLDLDNFKPLNDTHGHAVGDLLLVEVAIR
                     LKNCVRAMDTVARFGGDEFVVMLGELVADRNESTAQARIVAEKIRLALAEPYSLTVAH
                     ENQADTRVDHHCTTSIGAVVFIDHEATQDDILKWADAAMYRAKKAGRNS"
BASE COUNT         1462 a         2158 c         2048 g         1326 t
ORIGIN      
        1 tcaactacgc caaccagccc tacgatctgg tgctgctgga acgcgcgcgc gcggtggcgg
       61 agcaaatgaa gctgggcagc ggtcaggaga atctggattt caaacccaac ttgccgaacg
      121 gcgaaatgct cggcatgccg gaccgggtgt tctacaccgt gagcgacaag gacgggaaga
      181 agatcgtcgg caacgccaac ctgtcccgcc cgctggccta tcgcggcaag aagccggggc
      241 cggtgttcag caacagcgag cacgacggcg aaaaaacccg catggtcagt ctcgtctacc
      301 ccggcccgcc gggcgcgaag ctgtaccagt tgcacatgtc ggagaccatc caccagcgcc
      361 aggcgctgat acgcggcatt ctcgccaaca tcgtgatccc gcagttattg ctgctgctga
      421 tcgccggggg ggcgctgtgg tacgcgctga agcacggcct gcgtccgctg gaacgcctgc
      481 gtcaggacgt ggcgcggcgg cagcgcgacg acctgagccg actggatgag acccgcgcac
      541 cggcggaggt gcgcccgctg atcgcggcgg tcaacgatct gctggagcgg ctcaagcaag
      601 tcatgcagtc gcagaagcga ttcgtcgccg atgcggcgca ccagttgcgc accccgttcg
      661 cgggcctgaa aacacaggcc gaactggcgt tgcgcgagaa cgagatcgaa cgcaagcagc
      721 aggcgctcga acacatcctc accagcgccc ggcaaagcac ccatctggta aaccagttgc
      781 tggcgctggc gcgcaacgag ccgggcggtc aaggcgccga cagcttcgcc ccgctcgacc
      841 tcaatcatct ggcacaggaa tgtacggtgc gctgggtggc ccttgcgctg gaaaaaaaca
      901 tcgacctcgg cttcgagggc atcccggtgc aagcgccggt gcgcggcgac gcggacagcc
      961 tgacggaaat gctcggcaac ctgatcgaca acgcgatccg ctacacgccg gaaggcggac
     1021 acatcacggt cagcgtcgat taccagcgcg gcggcgcgat actgcaagtg gaagacaacg
     1081 gtccgggcat cccgccgcaa caccgcgagc gcgtgttcga acgtttctac cgcatcctcg
     1141 gcagcggaca aagcggcagc ggcctcggcc tcgccatcgt cgccgaggtc gcgaaacggc
     1201 acggcgcgac catcgcgcta ggcgaaggaa aaaacggtgc aggcacgctc atcagcatcc
     1261 gcttccccca tcacggcagt taagcctgcc ggattttagt tgtagctcga caactgtttt
     1321 tcgaactccg ccagcggcac tggcttgctg aacagatacc cctgatatgc cgaacacccg
     1381 tgctgctcca ggaatgaacg ctgcgcctcg gtttccacgc cttccgcgat gacttccatg
     1441 ccgaggttgt tggccatgcc gatgatggtt tgggcgatga ctgcatcgcc gggctttacg
     1501 ccaatatttc gtacgaatga ctggtcgatc ttcagctgat cgatcggcag ttgcgtcaaa
     1561 tacgccagcg atgaatagcc cgtgccgaaa tcatctacgg agaaacggat gcccaacgcc
     1621 cgcagttcgc gcattctgga aatggactcc tcgattcttt ccagcactgc actttcggtc
     1681 aactccagct tcaactgaaa tggatttgcg ccactctcaa gcagaacttg ccgcacctgg
     1741 gcaaggaagc cgggttggcg gaactgcctg gcgctaacgt taacagccag cgtcaaattg
     1801 cgcgtcagcg ggttctgacg ccaggccgcg agttgtgcgg ccgcagttcc aagcacccac
     1861 agacctatcg cctcgatgat cccgctttct tccgccagcg gaatgaactg ggcgggagat
     1921 atcagaccgc gttccggatg ttcccaacgg atcaacgctt cagctcccag cgaccggtgc
     1981 ttgctgtcca cctggatttg atagtgcagg cggaattgct gtttttcgag ggcctgacgc
     2041 aattcgcttt cgagttcggc gcgcgcctcc agcacggcct gcatatccgg atcaaagaaa
     2101 cggatggcat tgcgcccggc cgtcttggct tgatacatgg cggtgtcggc atgcttgagc
     2161 aaattatcca gattttcctg atgccccctg aacagtacga tgccgatact ggacgtgctg
     2221 tgatgcgaat attctttgat ctggtacggc tgattcaggg ccgcctgtat tttttctgcg
     2281 accgcttcgg cctgggtggc tgcttcatcc atattgatgc tgagcgtttc cagcacgacc
     2341 acgaattcat cgccgccaag gcgggccacc gtgtcgccct cgcgcacgca agacgctaaa
     2401 cgcctggaca cctcgatgag cagcaaatca ccgaattcat gccccctgct gtcgttgagc
     2461 attttgaagt tgtccagatc gaggaacatg atcgcgccat gctgtccatt gcgggcgctt
     2521 acagaaaagg cctggtgcag gcgatccatc agcaaacgcc ggttcggcag gtgcgtgagc
     2581 ggatcgaaga aagccagctg gtaaatttgc tgctcggcgc tcttgcgctc ggtaatatcg
     2641 cgggacagca cgatgaatcc cgcatcctga agcgagtcag ccggacgctt ggcgacagac
     2701 aactcgaacc agcaatctcc ttccggcaac tggagcctga tagaccgtcc gtgcgataag
     2761 cccttttcat ctgcttccga gattgcggca agcacctcct gggcagcaat taacggaagc
     2821 atctcttcga cggtgcgccc gatcagttga ttttccggtg ctgcgagcaa attgccccgg
     2881 ataccccata cgttcagata ccgtccgttg cgatccagct caaacatcaa atcaggcaca
     2941 gcctgcaata cggcttcgaa gcgttgattg ctgctcctga gctgttcttc gtttcgacgg
     3001 cgttcggtga catcttcaac gatggaccag atgaattttt cgccgtcgct ccccgttatc
     3061 tgaacgccat tcaggcgaac cgggacgcgt tgaccgctgc tgttgatgta ttccttttcg
     3121 taaggtccat acctggcatg cactctgaga gattccagtt gctcagcctc ctgttccgaa
     3181 tagctttccg gcgtgaggtc ccagtaactg agacgattaa gctcctccag tgaatagccg
     3241 acaatattca gcagcgcgtt gttggcttcg acaaaggttc catccatgga atttcggatc
     3301 atgcccaacg gagacatatc aaacattgcg cggagctttt cttcgctcaa gcgcaaatcc
     3361 tgatcaatct ttctggcatc ggtgacatcg atggttatgc agatcagata cagcggcttt
     3421 ccggacgagt cataaacagg cttcttgaag gtatggccaa tacgattttg cttgagcgtc
     3481 gcattccaga atgtttcttc aaaatcgacc tggctgccac ccgcaaatat ctccctgtcc
     3541 ttggcgagga accactccat ctggtctggc ggaaaaaatt ggctggcatc ggttccgcgc
     3601 aggtctgaaa aacgcattcc ccattgttct tcgcaagcgc tgttcatcag caggaacttg
     3661 ctgtcggcat cttttatgaa aatggcgatg ggcaaaacat cgaccaattg ctgcattgct
     3721 tttacatctt caagtatttg aggctgcatg aatatcctta atcttgcggg tgccaccgcc
     3781 gtgacaacgg gagctctcca ccagatctgt tacgacaatg attgcaaagc gcacgagtaa
     3841 gcaggggcaa cgataccacg acgaagctcc ggcaaactac ctcgacacat tgcatcccga
     3901 tatcgactat cgagccaaaa aatggttggc aacgggaacg ccagttgaat gtatccctgt
     3961 gcctcattga gcggtatcat aaaagcatga agaagctctc tcgccgtcaa ttcattgcat
     4021 cctcagccgg tttggctctc gcgcttgcca tccccggttg cgggtggctg gccgacaagc
     4081 ccatcgccat cgcagcgcat gtctggccgg gctacgagcc gatgttcctg gcgcgcagcg
     4141 aaggctggct ggatgccgaa caggtgcggc tggtcgaaac gacatcggcc accggctcgc
     4201 tgcaagccct ggccgacggc aaggtggacg gcgccgcgct gacgctcgat gaagtgttgc
     4261 ggggccgggc gatggggctg ccgctgtcgg tggtgatggt gttcgacgtt tcggccggag
     4321 ccgatgtatt gatcgcccgc cccggcatca aaaaactcgc ggacctcaag ggccgccgca
     4381 tcggttatga acccggtgcg ctgggcgcgc tgatgctggc ggaagcattg cgggcagccc
     4441 atctgacgca gaacgatgtg aaaccggttc cattcaccat cgacaagcaa cacgaagcct
     4501 ggatgaacga tcaggtggac gccctcatca cctacgagcc ggtatccagc cagttgctgt
     4561 cgcaaggcgc ggtcacgctg ttcgacagca ggcgcattcc caacaccatc atcgacgtgc
     4621 tggcggtgcg cggcgacata ctcgaccagc gccatgctgc ggccatccgc cacctgctca
     4681 gcgcgcattt ccgcgcgctg gatcacctga accgcaaccc gcaggatgcc gcattccgca
     4741 tggcaagcca catgaacctg cccgcttccg atgtgctgcc cgcattcaag gggctggtgc
     4801 tgccggatgc cgacaacaat cgccgcctgc tgacgggcgc tgcgccggaa ttgcgggaaa
     4861 ctgcaagcaa agtctcccat gtcatggtcg aaacgaaatt gctgccgcgc gacgacgaca
     4921 tgaaggggct gatacatgcc gaattcctgc cgtcgccaac cgcagagagc cacccatgaa
     4981 cctgcccgga tcgagccgcc cagagtcgat ccggggcctt gcggttattc tggcgctgct
     5041 ggccgtgctg atgtcggcgg cattgccggt ctcaccggcg catgccgccg agccggtgaa
     5101 aatcggcgtg ctggcgttcc gccccaagcc gcaaacgctg gcacagtggc aaccgctggc
     5161 gcaaatcctg aagaaagcca tccccgaacg ggatttcgtc atcgaggcgc tcaccaacca
     5221 ggaaatggat atggcggtcg ccagccgcca actggatttc gtgctgacca accccagcca
     5281 ttacgtgctg ctgtcgcacc atagcggcct gtcctcgccg cttgccacgc tcgctgaaga
     5341 tgcgcgaggc caggccgtca gcgtattcgg cggcgtcatc ttcagccggg cggatagcgc
     5401 cggcatcaat tcgctggctg acctgaaggg caaatccatc gccgcgctcg gcaccgaatc
     5461 gatgggcggt taccagatgc aggcgtatga gctgaagcgg caaggcatcc gtccgtcgca
     5521 ggaaatcagg ctgctcagcg tcggcgcgcc gcaggacaat gtggtgaacg aagtgctggc
     5581 aggccatgcc gaggccggtt ttgtgcgcac cggcgtgctg gaagccttgt cgcgcgaagg
     5641 caagctggat atggcgcagc tcaagatcat cgccccccag aatctgccgg gcttcccggc
     5701 gcaggtttcc acccggctct accccgaatg gccgtttgct gcgctgccgc atatcgacga
     5761 caaccttgcc cggcgcgtcg cggcggcact gctggtgctg gaacaaaact ccgccgccag
     5821 caagtcattg ggcatacacg gcttcgtcat tcccgccgac tacacgccgg tggaggacat
     5881 gatgcgcgaa ctgcgcatgc cgcccttcga tctggcgccg gaatttacgc tgaacgacgt
     5941 gtggaaccaa taccgctggc aggtgattgc cgcgctggct gcctgcggat tgatcgcgtt
     6001 tctgttggcg cgcttgctgt ggaccaaccg caggctggac gccgaacacc gcaccgtgct
     6061 ggagcaaaaa cagcagttgc aggagagcga ataccgctgg aaatttgcgc tcgaaggctc
     6121 cggcgacggc ctctgggact ggaacctcac ggacaacacc gtgttttact cgcggcgctg
     6181 gaaagagatg ctgggctatc gggaggatga aatcggcgac agtctcggcg agtgggacaa
     6241 acgcgtccat cccgacgaca aaaccgcaac gctgcaaacc ttgcaggccc atctggacgg
     6301 caagtccccc atgtatgtca gcgagcatcg cgtccacagc aaggacggcg actggaaatg
     6361 gatactcgac cggggcgtgg tggtcagccg cgacaaagac ggcaaacccc tgcgcatgat
     6421 aggcacgcac accgatgtca ccgaccgcca ccagatggaa gagcaggtgc gccaactggc
     6481 gctctacgac tcgctcacca aattgccgaa ccgtcgcctg ctcaacgagc gcctgagcca
     6541 cgtcatggca gccagcaagc gcagcggctg ctactgcgcg ctgatgttcc tcgatctgga
     6601 caacttcaag ccgctcaacg atacgcacgg gcatgcggtc ggcgacctgc tgctggtcga
     6661 ggtggcgatc cggctgaaaa attgcgtgcg cgcgatggac acggtggcgc ggttcggcgg
     6721 cgacgagttt gtcgtgatgc tgggcgaact ggttgcggac cgaaacgaat ccaccgcgca
     6781 ggcccgcatc gtcgcggaga aaatccgtct cgcgctggcc gagccctact ccctgaccgt
     6841 cgcgcacgaa aatcaggccg acacccgcgt cgatcatcac tgcacgacca gcatcggcgc
     6901 ggtcgtgttc atcgaccacg aagcgactca ggacgacatc ctgaaatggg ccgatgcggc
     6961 gatgtaccgg gccaaaaaag cgggccgcaa ttcg
//
LOCUS       DASUIV010000080         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1041381, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000080 DASUIV010000000
VERSION     DASUIV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041381"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1460
                     /locus_tag="VFW59_02930"
     CDS             288..1460
                     /locus_tag="VFW59_02930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5363198.1"
                     /translation="MSTELFDQITPENRAEVEAHCVRTLFERARQATRLAPLGTLFIF
                     WVVYATASLPLAIGWVIVNSLPDALTFVLTSRLLKKRPAGADIASSHRIQIMLRMIQG
                     LCWGSAVVVFHIPDDPTAFNDIAILAVLTAVTATNVVSMAPSFRTLAGFCLSTLLVPI
                     LYSLWLGDAIHIKIAVALILLLLVEIRFGWDAYRQFSGGVHQLVLNLKIRNQLEMRNV
                     ELDELNQKLRVIAIHDQLTGLYNRHFMVDQIERQRELFARHGNICSIVLFDIDHFKQI
                     NDRYGHAVGDDVLVAFSRRVEVLLRQGDALGRYGGEEFLLLLPMTDLNAALHLAERIR
                     CILADSPVILKSEEITVTASFGVAQLEPGEDADNWLIRADQALYRAKEHGRNCAMT"
     gene            1601..>2060
                     /locus_tag="VFW59_02935"
     CDS             1601..>2060
                     /locus_tag="VFW59_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5363199.1"
                     /translation="MSAVADYKLVRNSTVGSELTEEKARLLAAKMGVRHLKDGEFLVK
                     EGETDQTLFVLAAGRLSVISKDSDGVEQTVYTMKEGECAGTRAFVEQSPRRATLRAVG
                     DTTVYTLTPSDFESLLDSHPRVAFKVMRALFRVTHANLSRMNQESRELTNY"
BASE COUNT          445 a          595 c          593 g          427 t
ORIGIN      
        1 tggagcgtaa tggcttggat gttttggttt agccgatgtc gacgatacca atagagacag
       61 tcaccacaga aaatcgctcc attgcggggg atgcaaagtt gagtgcgcct gtgcgttctg
      121 gtgacactgc aaacaatggg ggcagattta tttgttttgg ttgaaaaata aatctgcccc
      181 ttttttctat ttacactgca aacaatgggg acagatttat ttgttttggt tgaaaaataa
      241 actagtcccc ttttttctat ttaaattttc tacacccaga atcgccgatg tccacagaac
      301 tattcgatca gatcacccct gaaaaccgtg ccgaggtcga ggcgcattgc gtgagaacct
      361 tgttcgagcg tgcgcgtcag gcgacgcggc tcgcgccgct ggggacgctg ttcattttct
      421 gggtcgtgta cgccacggcg agtctgccgc tcgccatcgg ctgggtgatc gtcaactcgt
      481 tgcccgatgc gctgacgttt gtacttactt cgcgtttgct gaagaagcgg ccagctggtg
      541 cagacattgc cagcagtcac cggatacaga tcatgctgcg catgatacag ggactgtgct
      601 ggggctcagc agtggtggtc ttccacatcc cggacgatcc gaccgcgttc aacgacatcg
      661 ccatcctcgc cgtgctgacc gcagtcaccg cgaccaacgt ggtcagcatg gcgccctcgt
      721 tccgcacgct ggcgggcttc tgcctatcca cgctgctcgt ccccatcctc tattcgctct
      781 ggctgggcga tgcgatccac atcaagatcg cggtcgccct gatcctcctg ctgctggtcg
      841 agatacgctt cggctgggac gcttaccgcc agttctccgg cggcgtgcat cagctggtgc
      901 tgaacctgaa gattcgcaat caactggaga tgcgcaacgt cgaactcgac gaactcaacc
      961 agaagctcag ggtcatcgcc atccacgacc agctcaccgg gctgtacaac cgccatttca
     1021 tggtggacca gatcgagcgg cagcgcgagc tgtttgcgcg ccatggcaac atctgcagca
     1081 tcgtgctgtt cgacatcgac catttcaagc agatcaacga ccgttacggc catgcggtgg
     1141 gcgacgacgt gctcgtggct ttcagccgcc gcgtcgaggt gttgttgcga cagggtgatg
     1201 cgctcggccg ctacggcggc gaagagttcc tgctgttact gccgatgacc gaccttaacg
     1261 cggcgctaca tctcgccgaa cgcatacgct gcatcctggc ggacagcccg gtgatcctca
     1321 agtccgagga aatcaccgtc accgcctcgt tcggcgtcgc ccagctcgaa cccggcgaag
     1381 acgcggacaa ctggctgata cgcgcagacc aggccctgta ccgggcgaaa gagcatggac
     1441 gaaattgcgc gatgacctaa cgatgcccgg catgcagaca aacagtggat tgccgggtat
     1501 aaatggcgac ctatataaaa aaccaaatgt cccggcatcg ccgagttggc tgaattgaaa
     1561 tgccgcactg tttcaccgac aaacccaaag gagttaagca atgagcgcag tcgcagacta
     1621 caaactggtt cgcaattcaa cggtgggctc cgagctgacg gaagagaagg ccaggctgct
     1681 cgcggcgaag atgggggtac gccatttgaa agacggcgaa ttcctcgtga aggaaggcga
     1741 gaccgatcag accctgttcg ttctggccgc aggcaggctc tccgtcatca gcaaggattc
     1801 cgacggtgtg gaacaaacgg tctacaccat gaaggagggc gagtgcgccg gcacccgagc
     1861 cttcgtcgag cagtcaccgc gcagggcgac gctacgggcg gtgggcgata ccacggtcta
     1921 cacgctgacc ccgtccgact tcgaatcatt gctggactca cacccgagag tggccttcaa
     1981 ggtgatgcgc gcgctgttcc gcgtgaccca cgcgaatctg tcgcggatga atcaggagag
     2041 ccgggagctg acgaattaca
//
LOCUS       DASUIV010000081         5297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_19155, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000081 DASUIV010000000
VERSION     DASUIV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19155"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /locus_tag="VFW59_02940"
     CDS             complement(<1..1958)
                     /locus_tag="VFW59_02940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5363200.1"
                     /translation="MHNLPSLLDEIGIDAEEIALRQDFLSMDERDNALLRNMHATLEG
                     QRGRLTEVFYEHLLRFPEIRPLLGDDAKLARLKSAQMKYFSQLTEGCYDQSYVENRLH
                     IGVVHQRVGLTPKWYMSAYCKYLNEVVPLIMAQYADAPEQGLDACRALVKVIFFDMGL
                     ALDTYFHAEKKELRVARSYTEQIVSNMPIGFIVLDAHGQIRLVNRAVLHMFELDDDEA
                     WRNRSLGDYLGSSDLDTKVSQVVSSRKHCNEFGFERKIDNKQRAFLADISLAQMGDEH
                     VVLLMIQDITRRKQSEEAVHRLAFYDSLTQLPNRRLLQERLRQSMSTSARSGMHGALM
                     FIDMDNFKTLNDTQGHDVGDLLLKEVAHRLMRNVREGDTVARLGGDEFVVALESLSAN
                     SHEAANHSETIAEKIRAELNQPYRLNGIEHHSSPSIGISLFRGHQNSLDEVLKQADLA
                     MYQAKASGRNMVCFFDPAMQTEMELRAELEKDLRASLRLNQLMLYYQMQVDDSGRIVS
                     AEALIRWKHPQRGMVSPAQFIPLAEETGMILPIGDWVIEQACAQLKIWEMDPVMRKIQ
                     LSVNVSPRQLSQPYFVEQVTEAIEKSGIRASQLKLELTESFILKDVEEAIEKMKELHW
                     IGVHFAMDDFGTGYSSLAYLNRLPLEQL"
     gene            complement(1961..3511)
                     /gene="nifA"
                     /locus_tag="VFW59_02945"
     CDS             complement(1961..3511)
                     /gene="nifA"
                     /locus_tag="VFW59_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HEX5363201.1"
                     /translation="MPNSMETCLEQNNEQLCAELKTLYQVSRVLSHSLNFKQTLDGVL
                     HALHEGMELQRGMVSLIDAVSGELQISLVYGVSDSVTEEVSYLPGEGVLGGILKSGDS
                     IAVECIMSEPRFLSRLGIYDPRGAFVGVPIRIGKKVVGVLAAQPAPSVAKRLSEYQRF
                     LEMVANLIGQSVRLSQEVAQEKMEITEERDVLRRAVRGQHGFDNIIGHTQPMRRIFEQ
                     VRLVAKWDTTVLIRGETGTGKELIANAIHYNSPRARGSFVKLNCAALPESLLESELFG
                     HEKGAFTGAVAQRKGRFEQAEGGSVFLDEIGEISASFQAKLLRVLQEGELERVGGTRT
                     IKVDVRVIAATHRDLEVEVEKGRFREDLYYRLNVMPLYLPPLRERMEDIPDIARFLVE
                     KVGTRQGRELTLTDTALRILMHHDWPGNVRELENCLERAAVMTEGLVIDRDAILLTGI
                     DEKISVSRGAIQTMDLDDPNLDEREKVIAALEQAGWVQAKAARLLDMTPRQIAYRIQT
                     LNIKVKQI"
     gene            complement(3504..5081)
                     /gene="nifL"
                     /locus_tag="VFW59_02950"
     CDS             complement(3504..5081)
                     /gene="nifL"
                     /locus_tag="VFW59_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HEX5363202.1"
                     /translation="MAKPPAPQASNKPAPDNHVPPHIFRQAVDQAALAISITDARANI
                     LYANAAFLRTTGYEAEEIVGHNESILSYRVTPKLVYETLWAQIQRQRPWNGLLVNKRK
                     DGSRYLADVTITPVVNDEGLTSHYLGMQRDVTEVHRLERQVQNQKLLIESVVDAAQVA
                     IVLLDEHEQVLLDNQEYKKLIVDLGKEPAVRLLAALRAQLGETFSKAYTQHRNIAARE
                     VCIELPNRATRWFSCAVSWFEEQDVGADAFYEPLRRHYLLLTVQDISELKQQQEALRI
                     NGLRALLSEQERIQGLRETLAGAIYQLEGPLNMLSATANMLERRLIQTSAAGTQNGAE
                     QISVSALEEAILKSREALETLRACMPEPGDAPLQQVDLNQLLSDVLKLMTASLLGAGI
                     VVEWLPSAAPAMIAGHSAQLSNLFKQLVVNAIEAVNERRGGKRELRIVCNHHGDHLEV
                     FVEDSGPAIADELRYKVFQPFFTTKGATGKHLGLGLAMAQEVVIRHGGTIDIDPGFQP
                     GCRLRVQLPCTTGGSHA"
BASE COUNT          940 a         1548 c         1598 g         1211 t
ORIGIN      
        1 ttgagttgct ccagtggcaa tcggttcaga taagccagcg aggagtagcc ggtgccgaaa
       61 tcatccatgg cgaaatgcac ccctatccaa tgcagttcct tcatcttttc gatggcttct
      121 tcgacatctt tcaggatgaa actttcggtc agttccagct tgagttggga ggcgcggata
      181 ccgctctttt caatggcttc cgtcacctgc tccacgaagt agggctggct caactgtctc
      241 gggctgacgt tgaccgagag ctgtatcttg cgcatgaccg ggtccatctc ccatatcttc
      301 aattgggcgc aagcctgctc gatgacccag tcgccgatcg gcaggatcat gccggtctct
      361 tctgccagag ggatgaactg agcgggcgag accatgccgc gttgcgggtg tttccagcgg
      421 atcagtgcct cggcgctgac gatgcggccc gagtcatcca cctgcatctg gtagtagagc
      481 atgagctggt tcaggcgcag ggaggcacgg aggtcctttt ccagttctgc gcggagctcc
      541 atctctgtct gcatcgccgg atcgaagaag cacaccatgt tgcgcccgga agccttggcc
      601 tgatacatgg cgaggtcggc ctgtttcagc acctcgtcca gactgttctg atggccgcga
      661 aacaggctga tgccgatgct gggcgaacta tggtgctcga tgccgttgag ccggtagggc
      721 tggttgagct cggcgcggat cttctcggcg atggtctcgg aatgattcgc cgcttcatgg
      781 gagtttgcgc tcaatgattc gagggcgacc acgaactcat ccccgcccag ccgcgcgacc
      841 gtatcgcctt cgcgtacgtt tcgcatcagg cgatgggcga cctctttcag cagcaggtcg
      901 ccgacgtcgt gaccctgcgt gtcgttgagc gtcttgaaat tgtccatgtc gatgaacatc
      961 agagccccgt gcatgccgct gcgcgcgctg gtggacatgc tctgtctcag gcgttcttgc
     1021 agcaggcgac ggttgggtag ctgggtgagc gagtcgtaga aggcgaggcg atgcaccgct
     1081 tcctcggact gtttacggcg cgtgatgtct tgaatcatca gcaggacgac atgttcatcg
     1141 cccatctgtg ccagagagat gtcggcaaga aaggcgcgct gcttgttatc gatctttcgt
     1201 tcaaacccga attcgttgca atgtttgcgg ctgctgacga cttgactgac cttggtgtcc
     1261 agatcacttg atccgagata atcgccaagc gacctgtttc gccatgcttc gtcatcgtcc
     1321 agttcaaaca tatgcagaac agccctgttc accagtctga tctggccatg agcatccaga
     1381 acgatgaagc caatcggcat gttgctgacg atctgctcgg tgtagctgcg agccacacgc
     1441 agttccttct tttcggcgtg gaagtaggtg tccagcgcca gtcccatgtc gaagaagatg
     1501 accttgacca gagcccggca tgcatccagc ccttgttccg gtgcgtcggc gtattgcgcc
     1561 atgattagcg ggacgacctc gttgaggtat ttgcagtagg cgctcatgta ccacttcggg
     1621 gtcagtccga cgcgctggtg tacgacgccg atatgcaggc ggttctccac ataggactgg
     1681 tcgtagcatc cttcagtcag ttgactgaag tacttcatct gggcgctctt cagtcgtgcc
     1741 agttttgcgt cgtcacccag cagcgggcgt atctcgggga aacgcaacag gtgctcgtaa
     1801 aagacctcgg tcaatctccc gcgctgacct tccagcgtgg catgcatatt tctcagcagc
     1861 gcgttatcgc gttcgtccat cgacaggaag tcctgacgca gagcgatctc ttcggcatcg
     1921 atgccgatct cgtccagcag ggacggtaag ttgtgcatgt tcaaatctgt ttcaccttga
     1981 tgttcagggt ctggatgcgg taagcgatct ggcgtggcgt catgtcgagc agacgcgcgg
     2041 ccttggcttg cacccagcct gcctgctcca gcgcggcgat gaccttttcg cgttcgtcca
     2101 ggttggggtc gtccaggtcc atggtctgga tggcgccccg gctcaccgag atcttttcat
     2161 cgatgccggt gagcaggatg gcgtcgcggt cgatcacgag accttcggtc atcacggcgg
     2221 cgcgttccag acagttctcc agttcgcgca cgttgccggg ccagtcgtga tgcatcagga
     2281 tgcgcagcgc cgtatcggtc agcgtcagtt cgcggccttg acgcgtgccg accttctcga
     2341 ccaggaagcg cgcgatatcc gggatgtctt ccatgcgttc gcgcaggggg ggcagataca
     2401 gcggcatcac attcaggcgg tagtacaggt cttcgcggaa gcggcctttc tctacctcga
     2461 cctccaggtc gcggtgggtc gcggcgatca cgcgcacgtc aacttttatc gtgcgggtgc
     2521 cgcccacgcg ctccagttca ccttcctgca gcacgcgcag cagcttggcc tggaacgagg
     2581 cagatatctc gccgatctcg tcgaggaaca ctgaaccgcc ttcggcctgt tcgaagcgac
     2641 ccttgcgttg tgcgaccgca ccggtgaacg cacctttctc atggccgaac agttcggatt
     2701 ccagcaggct ctctggcagc gcggcgcagt tgagtttgac gaagctgccg cgcgcccgcg
     2761 gtgagttgta gtggatggcg ttggcgatca gttccttgcc ggtgccggtc tcgccgcgga
     2821 tcagcacggt ggtatcccat ttcgcgacca gcctcacttg ttcgaagatg cggcgcatcg
     2881 gctgggtgtg gccgatgatg ttgtcgaagc cgtgctgtcc gcgcacggca cggcgcagca
     2941 catcgcgttc ttcggtgatc tccatctttt cctgtgcgac ttcctgcgac aggcgcacgc
     3001 tctgtccgat caggttggcg accatctcca ggaaacgctg gtattcgctg agccgtttcg
     3061 ccacgctggg agccggttgt gcggcaagta cacccaccac cttcttgccg atgcggatcg
     3121 gcacgccgac gaaggcgccg cgcgggtcgt agatgccgag acgcgaaagg aagcggggtt
     3181 cgctcatgat gcactcgacc gcgatgctgt cgccgctctt cagaatgccg ccgagcacgc
     3241 cttcacccgg cagatagctg acttcctcgg tgacgctgtc ggatacgccg tagaccagcg
     3301 atatctgcaa ttcgccgctc accgcgtcga tcaggctgac catgccgcgc tgcaactcca
     3361 tgccttcgtg tagagcatgc agcacgccat ccagtgtctg cttgaaattg agcgaatgcg
     3421 acaagacgcg gctgacctga tatagcgtct tcagctcggc gcagagctgc tcgttattct
     3481 gttctagaca ggtttccatt gagttaggca tgtgagcctc ctgttgtaca tggcagttgc
     3541 acgcgcaaac ggcaacctgg ctggaagccc ggatcgatat cgatggtgcc accgtgacgg
     3601 atcaccactt cctgagccat ggctaggccc aggccgagat gttttccggt agcgcccttg
     3661 gtcgtgaaga agggctggaa tactttgtag cgcagctcat cggcgatggc cggtccgctg
     3721 tcttcgacga acacttcgag gtgatcgcca tgatggttgc agacgatgcg cagttcgcgc
     3781 ttgccgccgc gccgttcgtt cacggcttcg atagcattga cgacgagctg cttgaacaga
     3841 ttggacagct gagccgagtg tcccgcgatc atcgccgggg ctgctgaagg cagccattcc
     3901 accacgatgc cagcgcctag taaggatgca gtcatgagct tgagcacatc ggacagcagc
     3961 tgattgagat cgacctgttg caagggagcg tcgcccggtt ccggcatgca ggcacgcagc
     4021 gtctccagtg cttcgcggct cttcaggatg gcttcctcca gtgcgctgac cgaaatctgc
     4081 tcggccccgt tctgggtgcc cgcagcggaa gtctggatca ggcggcgctc cagcatgttc
     4141 gcggtggcgc tgagcatgtt gagcgggcct tccagttgat agatcgcgcc ggccagtgtc
     4201 tcgcgcaatc cctgaatgcg ttcctgttcg gaaagcagcg cgcgcagccc gttgatgcgt
     4261 agtgcctcct gttgttgctt gagctcgctg atgtcctgca cggtgagcag cagatagtgg
     4321 cggcgcaacg gctcatagaa cgcatcggcg ccaacatctt gctcttcgaa ccatgacacc
     4381 gcacaggaga accagcgcgt ggcgcggttc ggtagttcga tgcagacctc gcgtgcggcg
     4441 atgttgcgat gttgcgtgta ggctttgctg aaagtctcgc ccagttgggc acgcaaggcg
     4501 gcgagcagtc tcactgcggg ttctttgccc agatcgacta tcagtttctt gtactcctga
     4561 ttgtccagca gcacttgctc atgctcatcc agcagcacga tggcgacctg tgccgcatcg
     4621 accaccgatt cgatcagcaa cttctggttc tgcacctgcc gttccagccg gtgcacttcg
     4681 gtcacgtcgc gttgcatgcc cagatagtgc gaggtcagac cttcatcgtt caccaccggg
     4741 gtgatggtga cgtcggccag ataacggctg ccgtccttgc gcttgttgac cagcaagccg
     4801 ttccaaggac gctgacgctg aatctgcgcc cacagggttt catacaccag cttgggcgtc
     4861 acacggtatg acaggataga ttcgttgtgt cccacgatct cctccgcttc atagcccgtg
     4921 gtacgcagga aggcagcatt ggcatacagg atattggcgc gtgcatcggt gatggaaatt
     4981 gcaagcgctg cctgatccac ggcctgacgg aagatgtgcg gaggcacatg attgtccggg
     5041 gcgggtttgt tcgatgcttg cggtgcagga ggcttggcca tgatggttcc atttcatgca
     5101 gatgcactct gttcagcaat atctgtgccc aaggttgctc gcctaggcgc gatgcctgcg
     5161 atgacggggg atttgtcggg tttgcgacat ccctgtcggc tatcgctcga ctgtgacaca
     5221 ggattttctg cctcaatttg gtgcgtgcac ctgtcaacag gccgagcgcg tgcaactgtg
     5281 tcggtcgccc gttctct
//
LOCUS       DASUIV010000082        11334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_762869, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000082 DASUIV010000000
VERSION     DASUIV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762869"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFW59_02955"
     CDS             complement(<1..453)
                     /locus_tag="VFW59_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5363203.1"
                     /translation="MYFIKPTDPYPVTPAKAVLLGLLILIWLGMGLVGHDPWKPDEIS
                     SFGIVYSMLQSGDWLVPTLLGDPFMDKPPLYYWTAALLAQLLEPLLPLHDGARLASGL
                     YVGLTLLFIGLAGRKLYGENRGWAAAIILIGCIGLLVRAHQMITDLALL"
     gene            complement(502..1923)
                     /locus_tag="VFW59_02960"
     CDS             complement(502..1923)
                     /locus_tag="VFW59_02960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5363204.1"
                     /translation="MRILIVAFALTLLSGCAQNPVTGRTDFVMMSENQEVAVGRQNDV
                     QVKQQYKVYESKALQDYVNHVGQKIAKKSHRPGLEYHFTVLDSPEINAFALPGGYVYI
                     TRGIMAYLNSEAELAAVLGHEIGHVTARHGVRQQSAAQATNIGLNVLSIFVPEVGSTV
                     GQNLSNIVSGALLSGYGRDHELEADRLGAEYLARTDYDPQAIIKVIGVLKNQELKDAE
                     LAKQEGREPRRYHGLFATHPDNDTRLQQAVGEAEKNAAEHAHFEGRGEYLSATDKLTF
                     GDDNEQGVVRNNHFYHGELGIALAFPAAWQVHNLPTSLVAVSPQGEATIQMKLDQKPS
                     GTPVEYANRLLGSGAKAQALEVNGLSAATFELPNTMGGVVYHNKNAYILQGQAKTKGS
                     LSPYRDEIFGIVRSFHPLTPEERKLVKPLDIRVITARTGDTYAKLAQGSPLGKSAESY
                     LRLINAQYPDGEPQAGQAIKIVE"
     gene            complement(2075..2290)
                     /gene="rpmE"
                     /locus_tag="VFW59_02965"
     CDS             complement(2075..2290)
                     /gene="rpmE"
                     /locus_tag="VFW59_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX5363205.1"
                     /translation="MKADIHPNYQELTVTCSCGNSFKTKSVIGKPTLGVEVCSECHPF
                     YTGTQKIVDTAGRIDKFRQKYGTKAAA"
     gene            2496..3749
                     /locus_tag="VFW59_02970"
     CDS             2496..3749
                     /locus_tag="VFW59_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5363206.1"
                     /translation="MSSLSSRDYRTLSLAALGGALEFYDFIIFVFFAVVIGQLFFPPD
                     MPDWLRQLQTFGIFAAGYLARPLGGIVMAHFGDLLGRKRMFMLSIFLMSVPTLLIGLL
                     PTYAVIGIWAPLALLVLRILQGAAIGGEVPGAWVFVSEHVPQRHVGFACGTLTAGLTG
                     GILLGSLVATAVNRIYSPADLLAEGWRVPFLIGGVFGLVSVWLRQWLHETPVFKEMQM
                     RQELADELPLKTVIRAHRPAVILTMLLTWLLSAAIVVMILMTPTLVQKLYAVPAATAL
                     QANSIATLFLSLGCMMFGWLADRYGAGRVMLAGCAMLGFSSLLFYKQMAVAPEHIYEL
                     YALCGFCVGVIGVVPSVAVKAFPPVVRFSGLSFSYNVAYAVFGGLTPVAVSMLLPLDP
                     LAPAHYVAVLSGVGMMVGAYLWRRG"
     gene            3854..4822
                     /gene="ispB"
                     /locus_tag="VFW59_02975"
     CDS             3854..4822
                     /gene="ispB"
                     /locus_tag="VFW59_02975"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEX5363207.1"
                     /translation="MFLDNLKQLLAADMAAVDAVIRARLHSEVALVNQVGEYIINSGG
                     KRLRPALVVLSAEAFGYQGKFQHDLAAVVEFIHTATLLHDDVVDESELRRGRETANAL
                     FGNAASVLVGDFLYSRAFQMMVDVGEMRVMQTLADATNVIAEGEVLQLLNCNDADVDI
                     PSYMRVIHYKTAKLFEAAMRLGAILGKASAAEEEAAARYGMHLGTAFQLIDDVLDYSA
                     EEAQTGKHLGDDLAEGKPTLPLIYAMQHGTPEQAAVVRSAIEQGDVGKFAEVLEIIHA
                     SGALEFTRQQALSEAEAACAQISALADSKYKQCLLELAHFAATRQF"
     gene            4839..4915
                     /locus_tag="VFW59_02980"
     tRNA            4839..4915
                     /locus_tag="VFW59_02980"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4873..4875,aa:Pro,seq:cgg)
     gene            complement(5033..6436)
                     /locus_tag="VFW59_02985"
     CDS             complement(5033..6436)
                     /locus_tag="VFW59_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5363208.1"
                     /translation="MNDAELNDCNQPSSYLASLNPATHKTNQTYRYWDVGHCEAALEK
                     THHAQQAWQQTPFSQRAEILRNVALHLLARRDRYATLITEEMGKPIREARAEIEKCAG
                     VCDYYVQHAEDFLRDEALASDATKSYVSHHPLGTVLAVMPWNFPFWQVFRAAVPALMA
                     GNALLLKHAPNVPQCAIALEEIFRRGGLPDGVFSNLMIPVDQVAEVIASPYVHAVTFT
                     GSEAAGRKVAECAGRHLKKCVLELGGSDPFIVLHDADMELTINMAVASRFLNCGQSCI
                     AAKRFIVVPEIADEFLSGLKAKVAGLKLGDPMNELTEIGPMARPDLRSVLHSQIADSI
                     AQGAVAVTGCHPVERDGFFYQPSILDRVTPKTRAYHEELFGPVACIIRATDEQDALRI
                     ANETRFGLGSSIWSKDSARAERLASEIQAGCTFINGMVKSDPGLPFGGVKASGYGREL
                     SRLGILEFVYAKTLWVR"
     gene            complement(6470..7126)
                     /locus_tag="VFW59_02990"
     CDS             complement(6470..7126)
                     /locus_tag="VFW59_02990"
                     /inference="COORDINATES: protein motif:HMM:NF042673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6629 family protein"
                     /protein_id="HEX5363209.1"
                     /translation="MCFSASASLIAGVLLSAIGVATISKTQHKNEIPLAAIPLLFGIQ
                     QFIEGFLWLSFRFEIPQLNSVMTYAFTFFSHVLWPAFVPFAILMVETVPWRRKIIWVF
                     QITGAAVGLYLLYYIVRHSISSEIHGHVIYVFPHFHKPLVLMLYVAATCAGAFFSSHK
                     ILNLFGVMSLFLFVVAYWFYKVAFFSVWCFFAAILSAIIYLHFKSSHARKPEVRLHGA
                     "
     gene            complement(7216..7401)
                     /locus_tag="VFW59_02995"
     CDS             complement(7216..7401)
                     /locus_tag="VFW59_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX5363210.1"
                     /translation="MNKDQVKGTVKDIAGKVQEEAGKLVGSKEQQVKGLGKQIAGKAE
                     KALGDAKEIIKSADKHS"
     gene            complement(7639..8910)
                     /locus_tag="VFW59_03000"
     CDS             complement(7639..8910)
                     /locus_tag="VFW59_03000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX5363211.1"
                     /translation="MAHVVIMGAGVGGMPAAYEMREKLDKSHKVTVVSNSENFHFVPS
                     NPWVGVKWRNRKQIEFPVRSYLERKGINFLSTGVKRVHPDQNQLELNDGAMLAYDYLV
                     IATGPKLAFDEVEGLGPHGGHTYSVCHIDHAMKSHDEWDDFCKNPGPIVVGAVQGASC
                     FGPAYEYAFIMETDLRKRKIRDKVPMTFVTSEPYIGHLGLGGVGDSKGILESGMRDKH
                     IKWICNAKVTKIEDGKMYVTEHDDMGQEKKKHELPFNYSMMLPAFKGVDAVFGIEGLT
                     NPRGFITVDKHQRNAKYKNIFAVGVCVAIPPVEPTPIPVGTPKTGYMIESMVTATVYN
                     IHADLVNEPADHEATWNAVCLADFGESGVAFVAIPQIPPRNVNWFSEGKWVHLAKIAF
                     EKYFIRKMKKGTSEPFYEGSIMKMLGIEKLK"
     gene            complement(9166..9846)
                     /locus_tag="VFW59_03005"
     CDS             complement(9166..9846)
                     /locus_tag="VFW59_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5363212.1"
                     /translation="MTDKLKVVLVDDQGLCRRGLSELLSNCYGFSVLGATGSIEELRT
                     LLDQKPDLLVLDLRMKPLDGVSLLEQLRREGYTTPAVMLTMSDSEADLSRAIRAGVRG
                     YLLKDMAPDDVVDAIRRVAAGELVVAPTMTIKMIELLRGDQPDQEPKSSLKLLTERER
                     EILQLLARGESNKAIAQELSISYDTVKQHVRHILTKLNLSSRVKAAVLFAKEQQGMPH
                     NDAPPPSC"
     gene            complement(9839..11074)
                     /locus_tag="VFW59_03010"
     CDS             complement(9839..11074)
                     /locus_tag="VFW59_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5363213.1"
                     /translation="MHKRTIPITHSPPSSFSWVRDPDFTPPDLDGGVDTCLTHEFAPP
                     RAGELRALLKTFLESIVGTVNATAGVVRLFSPDGRTLRIIGSAGLSAELQEEAENFVE
                     LDCEANDNATFGHVIHASDISTCDSRQSCRYASCRFQSLVAAPLESPNSPGIPLGILT
                     IFFDVPRESASQVMNTIAAFAEMMGAAIEHTRINRATSRMERLSARQEIANDVHDSLA
                     QTLTYARMRVSLLLEAIRNGNETAATQYAQDLDEALEIGQKSTRELISDFRCDMGPGG
                     LLAVLNEATALFRKHNDIELEYHNRLVDLELPLEHEIQISHIVREALTNIARHSGATH
                     ARLFVDANYGYYIFTIEDNGSGAATFTQVEGHYGVMIMRERAHRIGGDIRVDSTPNLG
                     TRVQLFFPEPSRNWRATND"
BASE COUNT         2068 a         3174 c         3518 g         2574 t
ORIGIN      
        1 gagcagcgcc agatcggtga tcatctggtg ggcgcgcacc agcaggccga tgcagccgat
       61 caggatgatc gccgccgccc agccgcgatt ctcgccatat agtttgcgtc cggcgaggcc
      121 gatgaacagc agcgtcagtc cgacgtagag gccgctggcg aggcgcgcgc cgtcgtgcag
      181 tggcagcagc ggctccagca gttgggcgag cagcgcggcg gtccagtagt acagcggcgg
      241 cttgtccatg aagggatcgc cgagcagcgt cggtaccagc cagtcaccgc tttgcagcat
      301 ggaatacacg atgccgaagc tgctgatctc atccggcttc catggatcgt gaccgaccag
      361 tcccatgccg agccagatca ggatcagcag gcccagcagc accgccttgg caggggtgac
      421 cggatagggg tcggtaggct tgatgaaata cattaaatcc tgctgtcccg ctcgctacgt
      481 tttttgttct gcatcatgcc gctattccac gatcttgatg gcttgtcccg cttgcggttc
      541 gccgtccgga tactgcgcat tgatcaggcg cagatagctt tctgccgact tgcccagcgg
      601 cgagccttgc gccaacttcg cataggtgtc gccggtgcgg gccgtgatga cgcggatatc
      661 gagcggcttg accagtttgc gctcctccgg ggtgagggga tggaagctgc gcacgatgcc
      721 gaatatctcg tcgcggtagg ggctcaggct gcccttggtc ttggcttgtc cctgcaggat
      781 gtaagcattc ttgttgtggt agaccacccc gcccatggtg ttgggtaact cgaatgtcgc
      841 ggctgacaat ccgttcactt ctagcgcctg ggctttcgcg ccggaaccca gcaggcggtt
      901 cgcgtattcg accggggtgc cgctcggttt ctgatccagt ttcatctgga tcgtcgcctc
      961 gccctgcggg ctgacggcga ccagcgaagt cggcaggttg tgtacctgcc aggcagccgg
     1021 gaaagccagc gcgataccga gttcgccgtg gtagaagtgg ttgttgcgta ccacgccctg
     1081 ttcgttgtcg tcgccgaagg tgagcttgtc ggtggcgctc aggtattcgc cgcgcccttc
     1141 gaagtgagcg tgttcggcag cgttcttttc cgcttcgccc accgcctgtt gcaatcgcgt
     1201 gtcgttgtcc gggtgggtgg cgaacaggcc gtggtagcgg cgcggctcac ggccttcctg
     1261 cttggccagt tcggcatctt tcagttcctg gttcttcagc acgccgatga ccttgatgat
     1321 ggcttgcggg tcatagtcgg tgcgcgccag atattcggcg ccgaggcggt cggcttccag
     1381 ttcgtgatcg cgtccgtagc ctgacagcag ggcgccgctg acgatattgc tgagattctg
     1441 gcctactgtc gatcccactt cgggtacgaa gatcgacagg acgttcaggc cgatgttggt
     1501 ggcctgcgcg gcgctctgct ggcgcacgcc gtggcgcgcg gtgacgtgac cgatctcatg
     1561 gcccagcacg gcggccagtt cggcctcgct gttgaggtag gccatgatgc cgcgcgtgat
     1621 gtagacgtag ccgccgggca gggcaaaggc gttgatctcg ggcgagtcga gtacggtgaa
     1681 gtgatattcc agaccgggac gatggctctt cttggcgatc ttctggccga catggttgac
     1741 gtaatcctgc agggccttcg attcgtagac cttgtattgc tgtttgacct gaacgtcgtt
     1801 ctggcgaccg acggcgactt cctgattctc cgacatcatc acgaaatcgg ttcttccggt
     1861 gaccggattc tgcgcgcagc cggacagtag tgtgagagca aaggcaacga ttaggatacg
     1921 catggcaatt cctgttcaga tcgaggcaaa gggggatagg cgcagcaaat aaaaaaggca
     1981 gcaaacgctg cctttagata aatccgccga gcgaagttcc cgatggaact cctgccaccc
     2041 ggccatccga tcagaacggt cgggcggctg ttgattacgc agcagccttg gtgccgtatt
     2101 tctggcggaa tttatcgata cggccagcgg tgtcgacgat cttctgggtg ccggtgtaga
     2161 acgggtgaca ctcggagcat acctcgacgc ccagtgtcgg cttgccgatc acggacttgg
     2221 tcttgaagct attgccacag ctgcaagtta cggtgagttc ctgataattc gggtggatgt
     2281 ctgctttcat ttttctttcc ttgcgagtag ttttgtgcgg cgggtgtacg cactaatgat
     2341 ggtgcataag ggggcgcatt atccataaaa atagggggtt gggcaaattt ttgtacggcg
     2401 ctcccgccgc agtgccgggc tgctgtcgcg ccgggctggc cctgatggcc ggcatcacct
     2461 aaactcccgt tctcaccacc cgaagcctag catccatgtc cagtctgagc agcagagatt
     2521 atcgcaccct gtcgttggcc gccctgggcg gcgcgctgga gttctacgac ttcatcatct
     2581 ttgtgttctt tgccgtcgtc atcggccagt tgttcttccc gccggacatg ccggactggc
     2641 tgcgccagtt gcagactttc ggcatcttcg cggcgggcta cctggcccgt ccgctgggcg
     2701 gtatcgtgat ggcgcacttc ggcgatctgc tgggacgcaa gcgcatgttc atgctgagca
     2761 tcttcctgat gtcggtgccg acgctgctca tcggtctgtt gcctacctat gccgtcatcg
     2821 gcatctgggc gccgctggcc ctgctggtct tgcgcatcct gcaaggggcg gccatcggtg
     2881 gcgaggtgcc gggtgcctgg gtgttcgtct ccgagcatgt gccgcagcgc catgtcggtt
     2941 tcgcctgcgg cacgctgacg gcggggctga ctggcggcat cctgctcggc tcgctggtgg
     3001 cgacggccgt caaccggatt tatagcccgg cagatttgtt ggcggaggga tggcgtgtgc
     3061 ccttcctcat cggcggcgta ttcggattgg tgtcggtctg gttgcgccag tggttgcatg
     3121 aaacaccggt gttcaaagag atgcagatgc gtcaggaact ggccgacgag ctgccgctga
     3181 agacggtgat acgcgcacat cgcccagccg tgatcctgac gatgctgctg acctggctgc
     3241 tctccgctgc gatcgtggtg atgatcttga tgacgccgac gctggtgcag aagctgtatg
     3301 ccgttcctgc cgccaccgcg ctgcaggcca acagcatcgc gacgctgttt ctgagtctgg
     3361 gctgcatgat gttcggctgg ctggcagacc gctacggtgc gggacgggtg atgctggcag
     3421 gatgcgcgat gctgggattc agctccctgc tgttctacaa gcagatggcc gtcgcgcccg
     3481 agcatatcta tgaactgtat gctttgtgcg gtttttgcgt cggcgtgatc ggcgtggtgc
     3541 cgagcgtcgc ggtcaaggcg tttccgcccg tggtgcgctt ctccggattg tctttttcct
     3601 acaatgtcgc ctacgccgta ttcggcggac tcacgccggt cgcggtgagc atgctgctgc
     3661 cgctcgatcc gctggctccc gcccattacg tggcggtgtt gagcggggtg ggaatgatgg
     3721 tgggtgctta cctgtggcgt cgcggctagt gcgccgtgtc ggcctgtgcg acaatgcagc
     3781 ccgcaaaccg gcatggacgc acctggtgcg ttgctccgta tccctttaac tcttaagaat
     3841 ctcagggcag gacgtgtttc tggataacct caagcaattg ctcgcggccg acatggccgc
     3901 agtggacgcg gtgatacgcg cccgtctgca ttccgaagtg gcattggtca atcaggtcgg
     3961 cgaatacatc atcaacagcg gcggcaagcg tttgcgtccc gcgctggtgg tgctgtccgc
     4021 cgaggcgttc ggttatcaag gcaagttcca gcacgatctg gccgccgtgg tcgaattcat
     4081 ccacaccgcg acgctgctgc atgacgacgt ggtggatgaa tccgagttgc gtcgcgggcg
     4141 cgaaaccgcc aatgcgctgt tcggcaatgc cgccagcgta ctggtcggcg acttcctgta
     4201 ttcgcgtgcg ttccagatga tggtcgatgt gggcgagatg cgcgtgatgc agacgctggc
     4261 ggatgccacc aatgtgatcg ccgagggcga ggtgttgcaa ctgctcaact gcaacgatgc
     4321 ggatgtcgat atccccagct acatgcgcgt cattcattac aagaccgcca agctgttcga
     4381 ggcggcgatg cgtctgggcg cgattctggg caaggcatcc gccgcagaag aagaggcggc
     4441 cgcgcgttac ggcatgcatc tgggtaccgc gttccagttg atcgacgatg tgctggacta
     4501 ttccgccgaa gaggcgcaga ccggcaagca tctgggggac gatctggccg aaggcaaacc
     4561 cacgttgccg ctgatctacg cgatgcagca cggcacgccg gaacaggccg ccgtggtgcg
     4621 ctccgcgatc gagcagggcg atgtgggcaa gttcgccgag gtgctggaga tcattcacgc
     4681 cagcggcgcg ctggagttca cccgccagca ggccttgagc gaagccgagg cggcatgcgc
     4741 gcaaatttct gcgctggccg attcaaaata taagcagtgt ttgttagaat tggcgcactt
     4801 cgccgcgacg cggcaattct agttgttcct gtttcactcg gagtgtggct cagcctggta
     4861 gagcactgcg ttcgggacgc aggggtcgga ggttcgaatc ctctcactcc gaccaatcag
     4921 tctattggtt aggtcgtttt atccatctcg gctttacacc cccagcctgt aaaaatgagc
     4981 ctctgattgt caggagcgtg tacaacctga cgacccaaga tttacgttgg cgctatcgca
     5041 cccacagggt ttttgcatag acgaattcaa gtatccccag tctggatagc tcgcgtccgt
     5101 agcccgacgc tttcactccg ccaaacggca agcccggatc ggatttgacc atgccgttga
     5161 tgaaggtgca gcctgcctga atttctgatg ccagccgttc cgcgcgtgcc gagtctttgc
     5221 tccagatcga cgacccaagc ccgaagcggg tttcgtttgc gatgcgtagc gcatcttgct
     5281 cgtctgtcgc gcgaatgatg caggcaaccg ggccgaacag ctcttcgtgg taagcgcggg
     5341 ttttcggcgt gacgcgatcc agaatggagg gctgatagaa gaacccgtcc cgttcgacag
     5401 gatgacatcc ggtaacggcc accgcgcctt gcgcgatgga gtcggcgatc tggctgtgca
     5461 atacgctgcg caaatcggga cgcgccatcg ggccgatctc ggtgagctcg ttcatggggt
     5521 cgcccagttt cagtccggcg acttttgcct tcaatccgct caggaattca tccgcgatct
     5581 cagggacgac gatgaagcgt ttggcggcga tgcaggattg gccgcaattg agaaagcgcg
     5641 aagccacggc catgttgatc gtgagttcca tatccgcatc gtgcagcacg atgaacggat
     5701 ccgatccgcc cagttccagc acgcatttct taagatgtcg gcccgcgcat tccgccactt
     5761 tgcgacccgc agcctccgag ccggtgaacg tgacggcatg tacatagggg ctggcgatta
     5821 cctcggccac ctgatcgacg gggatcatca ggttgctaaa cacgccgtcc ggaagaccgc
     5881 cgcgacggaa aatctcttcc agtgcgatgg cgcattgcgg gacgttcggc gcgtgtttca
     5941 gtaacagtgc attgcccgcc atcagtgccg gaaccgcagc acgaaatacc tgccagaagg
     6001 gaaagttcca gggcatgacg gccagcacgg tgccgagcgg atggtggctg acatagcttt
     6061 ttgttgcgtc cgacgccaac gcttcgtcgc gtaaaaaatc ttccgcgtgc tgcacatagt
     6121 agtcacacac cccggcgcac ttctctattt ctgcgcgcgc ctcgcgaatg ggtttgccca
     6181 tctcttcggt gatcagcgtt gcatagcggt cgcgtcgtgc gagcagatga agcgccacgt
     6241 tccgtaatat ttccgcccgc tgcgagaacg gagtctgttg ccaggcctgc tgggcgtggt
     6301 gagttttttc cagcgcagcc tcgcaatgac cgacatccca gtagcggtag gtttggttcg
     6361 ttttatgggt cgccggattg agactggcca gataggatga cggttggttg caatcgttta
     6421 attctgcatc attcattgag tgcgactccc gatgattcga cgcccggtct catgcgccgt
     6481 gaagtctcac ttctggcttg cgcgcatggc tcgatttgaa gtgcagataa atgatcgcgc
     6541 tcagtatcgc cgcgaaaaag caccatacgg aaaagaaggc gactttatag aaccagtatg
     6601 cgaccacaaa caggaacagc gacatgacgc caaacaggtt gagtattttg tggctggaaa
     6661 aaaaggcccc ggcacaggtg gccgccacat agagcatcaa taccagcggt ttgtggaaat
     6721 ggggaaatac gtaaatgaca tgtccatgga tttcggagct gatcgagtgc cggacgatgt
     6781 agtaaagcag atacaggccg actgcggctc cggtgatctg gaacacccag attatctttc
     6841 ttcgccaagg gacggtttcc accatgagaa tcgcaaatgg cacgaatgcc ggccagagta
     6901 catgcgaaaa aaaagtgaac gcataggtca tgacggaatt aagctgcggg atttcgaacc
     6961 ggaacgacag ccacagaaaa ccctcgatga attgctgtat cccgaacaat agtgggatcg
     7021 cggccagcgg tatttcattt ttgtgctgtg tcttgctgat ggtcgccaca ccgattgcgg
     7081 ataataagac tccggcgata aggctggcgg atgctgaaaa gcacatcgtg cttgatccct
     7141 tcttgagatt tgagcgtttg tgcggcctgc cggataaaac aatggccgcg tatagcagcc
     7201 atttttatgt ccgtttcagg aatgcttgtc ggcgctcttg atgatttcct tggcatcgcc
     7261 cagtgccttt tcagccttgc ccgctatttg cttgcccaga cctttgactt gctgttcttt
     7321 gctgcctacc agtttgccag cctcttcctg aactttgccg gcgatgtctt ttacggtgcc
     7381 tttgacttga tctttgttca tttgcattcc cctttaattg ctgcgattgc cgcgaacggt
     7441 aaggattgaa gaatgagcga aaagcatggg gaaaactgtg cgctaacgaa ccgaataaac
     7501 aatcggcagt ttcgactgag cttgaatctt aagcagcgtg ccgtcgcgat gccgtttccg
     7561 ggacgaatcc ggcagcaaat aaaaagggcc gttgtcagca tccgatgcag acaacggccc
     7621 cgctgtgttg cgcctcgctt acttcagttt ttcgatgccc agcatcttca tgatgctgcc
     7681 ttcatagaac ggctcggaag tgcctttctt catcttgcga atgaagtact tctcgaaagc
     7741 gatcttggcc agatgcaccc acttgccttc ggagaaccag ttcacgttgc gcggcgggat
     7801 ctgcgggatc gcgacgaagg ctacgccgct ctcgccgaag tcggccagac agaccgcgtt
     7861 ccaggtcgct tcgtggtcgg ccggttcgtt aaccagatcg gcatggatgt tgtataccgt
     7921 ggcggtcacc atggactcga tcatgtagcc ggtcttcggt gtgccgaccg ggatcggagt
     7981 cggttcgacc ggagggatgg cgacgcagac gccgacagcg aagatgttct tgtacttcgc
     8041 gttgcgctgg tgcttgtcga cggtgatgaa gccgcgcgga ttggtcagcc cttcgatgcc
     8101 gaacaccgca tccacaccct tgaaggcggg cagcatcatg ctgtagttga acggcagttc
     8161 gtgcttcttc ttctcctgcc ccatgtcgtc atgttcggtg acatacatct tgccatcctc
     8221 gatcttggtg accttggcat tgcagatcca cttgatgtgt ttgtcgcgca tgccggattc
     8281 gaggatgcct ttggagtcgc ccacgccgcc caggccgagg tggccgatgt agggctcgga
     8341 ggtgacgaag gtcatcggca ccttgtcgcg gatcttgcgt ttgcgcagat cggtttccat
     8401 gatgaaggcg tactcgtagg cggggccgaa gcaggatgcg ccctgtaccg cgccgaccac
     8461 gatggggccg gggttcttgc agaaatcatc ccactcgtcg tgcgatttca tcgcatggtc
     8521 gatatggcag accgagtagg tatggccgcc gtgcgggccg agcccctcga cttcgtcaaa
     8581 ggccagtttc ggtccggtgg cgatcaccag ataatcgtaa gcgagcattg cgccgtcatt
     8641 cagttcgagc tgattctggt cgggatggac gcgtttgacg ccggtggaga ggaagttgat
     8701 gcccttgcgc tccagatagc tgcgcaccgg gaattcgatc tgtttgcggt tgcgccattt
     8761 cacgccgacc caggggttgg aggggacgaa gtggaagttc tcgctgttcg agacgaccgt
     8821 caccttgtgg ctcttgtcca gtttctcccg catttcgtaa gctgccggca tgccgccgac
     8881 acctgctccc atgattacaa catgtgccat gttttcccct ccgttccggt tttgttgaga
     8941 agtatcgctc cgagtttgtc tttgcaagcc actgccgacg gtcatttgga gctttcttgg
     9001 tgcgcgcgtt gcgtgccgtc accaatgccc tattaggctt aatcgctcgc cggagtgtct
     9061 atagccacat tgggttagtg ggatataacc ccaatggtga gaggtgcgca tgaggtggaa
     9121 aagactgtcc tgaatgcgct tcaggatagg cctggttcga gtgctttagc agcttggagg
     9181 tggggcatca ttgtgcggca tgccttgttg ctccttggca aacagcaccg cagccttgac
     9241 gcgtgaggac aggttgagtt tggtcagaat gtggcgcaca tgctgcttga cggtgtcgta
     9301 gctgatcgac agctcctgcg cgatggcctt gttgctctcg ccgcgcgcca gcagttgcag
     9361 gatttcgcgc tcgcgttcgg tgagcagctt aagcgagctc ttgggttcct ggtcgggctg
     9421 gtcgccgcgc agcagctcga tcatcttgat ggtcatcgtg ggggcgacca ccagctcgcc
     9481 ggcggcgacg cggcggatgg catcgaccac atcgtccggt gccatgtcct tgagcaggta
     9541 gccgcgtacg ccggcacgga tggctcggga caggtcggcc tcggagtcgc tcatggtgag
     9601 catcacggcg ggagtggtgt agccttcgcg gcgcagctgt tccagcagcg agacgccatc
     9661 cagcggtttc atgcgcagat ccagcaccag cagatcgggc ttctgatcca gcagcgtgcg
     9721 cagctcttct atgctgccgg tcgcgccgag tacgctgaaa ccgtagcaat tggacagcaa
     9781 ttcgctcagt ccgcgtcggc acaggccctg atcgtcgacc agcaccactt ttaatttatc
     9841 agtcattcgt tgctctccag ttccgtgagg gttcggggaa aaatagctgc acccgggtgc
     9901 ccagattcgg ggtgctatcc accctgatgt cgccgccgat gcgatgcgcc cgttcgcgca
     9961 tgatcatcac tccgtaatgc ccctcgacct gagtaaaggt ggctgcaccg ctgccgttgt
    10021 cttcgatggt gaagatgtag tagccatagt ttgcgtcgac gaacagtctt gcatgagtcg
    10081 caccggaatg gcgggcgata ttggtcagcg cttcgcgcac gatatgcgat atctgaatct
    10141 cgtgctccag cggcagctcc aggtcgacca gccggttgtg atactcgagc tcgatgtcat
    10201 tgtgttttct gaacagggcc gtggcctcgt tcaggaccgc cagcaggccg ccgggcccca
    10261 tgtcgcagcg gaagtccgag atcagctctc gcgtgctctt ctggccgatc tccagcgcct
    10321 cgtccagatc ctgcgcatat tgggtggcgg cggtttcatt gccattgcgt atcgcttcga
    10381 gcagcaggct gacgcgcatg cgcgcatagg tcagcgtctg tgccagcgaa tcgtggacgt
    10441 cgttggcgat ctcctgacgc gcggacagcc gctccatgcg gctggtcgcg cgattgatac
    10501 gggtatgttc gatggcggca cccatcatct cggcgaaggc cgcgatggtg ttcatgacct
    10561 gactggccga ttcgcgcggc acgtcgaaga atatcgtcag gatgcccagc gggatgccgg
    10621 gggaattggg tgactcgagg ggggcggcga ccagcgactg gaaacggcaa ctggcgtaac
    10681 ggcagctctg gcgcgagtcg caggtgctga tatccgaggc gtggatgacg tggccgaagg
    10741 tggcgttgtc gttggcttcg cagtccagtt cgacgaaatt ttcggcctct tcctgcaact
    10801 cggccgacag tcctgccgag ccgatgatgc gcagggtgcg tccgtcggga gagaacagtc
    10861 gcactacgcc cgcagtcgca ttgaccgtgc cgacgatgct ttccaggaag gtcttcagca
    10921 gcgcgcgcaa ctcgccggca cggggaggcg cgaactcgtg ggtcagacag gtatccactc
    10981 cgccatccag atcgggaggc gtaaagtcgg gatcacgtac ccaggagaag ctggacgggg
    11041 gtgaatgggt tatgggaatc gttcttttgt gcattaattt tcattaattc ggcgggaggt
    11101 tccgcgagaa acgttcagca gagtattctt atttaccaag attatcaaca gattaaattg
    11161 ccgcgagggg cggtcacccc atcggggtta tgtgccgtct cacccgatgt gggtatagac
    11221 gtcgggcaag cgattgcgcc taaatggcga ggttttaaac gctcgcagcg tgggctttcc
    11281 gcgctattgc tgcgatatgc aaattcaacg acgacagatt atgccatcgc ccat
//
LOCUS       DASUIV010000083         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_484841, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000083 DASUIV010000000
VERSION     DASUIV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484841"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3009
                     /gene="carB"
                     /locus_tag="VFW59_03015"
     CDS             <1..3009
                     /gene="carB"
                     /locus_tag="VFW59_03015"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5363214.1"
                     /translation="WKMVEKIIAKERPDAILPTMGGQTALNCALDLAKHGVLEKYNVE
                     MIGASREAIDMAEDREKFKQAMTRIGLASARSAIAHSMEEALQVQQVMGYPTIIRPSF
                     TMGGSGGGIAYNREEFMEICERGLEASPTKELLIEESLLGWKEYEMEVVRDRNDNCII
                     ICSIENLDPMGVHTGDSITVAPAQTLTDKEYQILRDASLAVLREIGVETGGSNVQFSI
                     NPEDGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELQNDITGGATPA
                     SFEPTIDYVVTKIPRFAFEKFPQANDRLTTQMKSVGEVMAIGRTFQESFQKALRGLEV
                     GVNGLDTKFSDREAICAELGNPGPDRIWAVSDAFRIGMSVEEVFNVSKIDPWFLVQIE
                     DLIRQEQSLTGRTLESLNADDLRGLKRSGFADARLAFLLDTDDAAVRAYRHALGVRPV
                     FKRVDTCAAEFSTDTAYMYSTYEEECESQPTNNKKIMVLGGGPNRIGQGIEFDYCCVH
                     AALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEVVAVEKPIGVIVQY
                     GGQTPLKLAHGLEKNGVPIIGTTPDMIDCAEDRARFQAMLRKLELKQPPNRIASNVAD
                     AVAGAGEIGYPLVMRPSNVLGGRGMEIIHAQPDLERYMREAEELSKTYPERMPILLDR
                     FLNDAIEVDVDAVSDGKHVIIGGIMEHIEQAGVHSGDSACSLPPYSLSVALQDELRRQ
                     TIAMAKELNVVGLMNVQFAIQGETVYVLEVNPRASRTVPYVSKATGVPLAKIAARCMA
                     GQSLAQQGVTREVIPPYYSVKEAVFPFIKFPGVDTILGPEMKSTGEVMGVGETFAEAF
                     VKSQLAASVKLPKDGKVFISVRDADKAGAVEVARSLVELGFTILATRGTAVAITAANV
                     PVTVVNKVAEGRPHIVDMIKNGEVSLIINTVDSKPSAMRDSYSIRHAALQGRVTYYTT
                     LAGARAACVGMQHLTDLQVYDVQTQHKRLA"
     gene            3023..3499
                     /gene="greA"
                     /locus_tag="VFW59_03020"
     CDS             3023..3499
                     /gene="greA"
                     /locus_tag="VFW59_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5363215.1"
                     /translation="MSKIPLTLVGAELLRQELHNLKTVERPSVINAISEARAQGDLSE
                     NAEYEAAKERQSFVEGRIVELEFKLGSAQVIDPKTLNTDGRCVFGTTVVLEDVNNGDV
                     VTYQIVGDDEADIRKRKISISSPIARALIGKSSGDIAEVKAPGGVREYEVVEVQYV"
     gene            complement(3656..4006)
                     /locus_tag="VFW59_03025"
     CDS             complement(3656..4006)
                     /locus_tag="VFW59_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEX5363216.1"
                     /translation="MLSLTVAERLALKGRAHALNPTVMIGNAGLTESVLKEIALNLKA
                     HELIKIRVMAERPQREAIMTDICTQLNAAPVQHIGKILVIYLPNPEANQIELKKMPRH
                     KGKKPLTKKQLGSR"
     gene            4043..>4179
                     /locus_tag="VFW59_03030"
     CDS             4043..>4179
                     /locus_tag="VFW59_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEX5363217.1"
                     /translation="MKPSKTSKQWMREHLSDPFVQMAQKEGYRSRAAYKLLEIDAKDH
                     LL"
BASE COUNT          852 a         1201 c         1334 g          792 t
ORIGIN      
        1 tggaagatgg tcgagaagat catcgccaag gagcgtccgg atgcgatcct gccgaccatg
       61 ggcgggcaga ccgcgctgaa ctgcgcgctc gatctggcca agcacggcgt gctggaaaaa
      121 tacaacgtcg agatgatcgg cgcgtcgcgc gaggccatcg acatggccga agaccgcgag
      181 aagttcaagc aggcgatgac ccgcatcggt ctggcttcgg cgcgttccgc gatcgcgcac
      241 agcatggagg aggcgctgca ggtgcagcag gtgatgggtt atccgaccat catccgtccg
      301 tccttcacca tgggcggctc cggcggcggc atcgcctaca accgcgaaga gttcatggag
      361 atctgcgagc gcggtctgga ggcttctcct accaaggagc tgctgatcga ggaatcgctg
      421 ctcggctgga aagaatacga gatggaagtc gtgcgcgacc gcaacgacaa ctgcatcatc
      481 atctgctcca tcgaaaacct cgacccgatg ggcgtgcata ccggcgactc gatcaccgta
      541 gcacccgcgc agaccctgac cgacaaggaa taccagatcc tgcgtgacgc ctcgctggct
      601 gtgctgcgcg agatcggcgt ggagaccggc ggttccaacg tgcagttctc catcaacccg
      661 gaagacgggc gcatggtggt catcgagatg aatccgcgcg tgtcgcgttc ctcggcgctg
      721 gcctccaagg cgaccggctt cccgatcgcg aaagtcgcgg ccaaactggc cgtcggttac
      781 acgctggacg agttgcagaa cgacatcacc ggcggcgcga ccccggcttc gttcgagccg
      841 accatcgatt acgtggtgac caagatcccg cgtttcgcct tcgagaaatt cccgcaggcc
      901 aacgaccgcc tgaccacgca gatgaagtcg gtgggcgagg tgatggcgat cggccgcacc
      961 ttccaggagt cgttccagaa agccttgcgc ggactggaag tcggcgtgaa cggactggac
     1021 acgaaattca gcgaccgcga ggcgatctgc gccgagctcg gcaatcccgg ccctgaccgt
     1081 atctgggcgg tgagcgatgc gttccgcatc ggcatgagcg tcgaggaagt attcaacgtc
     1141 agcaagatcg atccgtggtt cctggtgcag atcgaggatc tgatccgtca ggagcaatcg
     1201 ctgaccggcc gcacgctgga aagtctgaac gccgatgacc tgcgcggctt gaagcgcagc
     1261 ggtttcgccg atgcgcgtct ggccttcctg ctggacaccg acgatgcggc ggtgcgtgcc
     1321 tatcgtcatg cgctgggcgt gcgtccggtg ttcaagcgcg tggatacctg cgccgccgag
     1381 ttttcgaccg acacggccta tatgtattcg acctacgagg aagagtgcga gtcgcagccg
     1441 accaacaaca agaagatcat ggtgctgggc ggcggtccga accgcatcgg ccagggtatc
     1501 gagttcgact attgctgcgt gcatgccgca ctggcgatgc gcgaggacgg ctacgagacc
     1561 atcatggtca actgcaatcc cgagaccgta tcgaccgact atgacacctc cgaccgcctg
     1621 tatttcgagc cgctgacgct ggaagacgtg ctggaagtcg tcgcggtcga gaagcctatc
     1681 ggcgtgatcg tgcagtacgg cggtcagacc ccgctgaagc tggctcacgg actggagaag
     1741 aacggcgtgc ccatcatcgg caccacgccg gacatgatcg actgcgcgga agatcgcgcg
     1801 cgtttccagg ccatgttgcg caagctcgaa ttgaagcagc cgccgaatcg cattgcgagc
     1861 aacgtggccg atgcggtcgc cggtgcaggc gagatcggct atccgctggt gatgcgccct
     1921 tccaacgtgc tgggcggtcg cggcatggag atcatccatg cgcagccgga tctggaacgc
     1981 tacatgcgcg aggccgaaga gctctccaag acttatcccg agcgcatgcc tatcctgctc
     2041 gaccgcttcc tgaacgatgc gatcgaagtg gatgtggacg cggtgtccga cggtaagcac
     2101 gtgatcatcg gcggcatcat ggaacatatc gagcaggcgg gcgtgcactc cggcgactcg
     2161 gcctgttcgc tgcctccgta ttcgctgtca gttgcgctgc aggacgagct gcgccgccag
     2221 accatcgcga tggccaagga gctgaacgtg gtcggcctga tgaacgtgca gttcgcgatt
     2281 cagggcgaga ccgtctatgt gctggaagtg aatccgcgcg cctcgcgcac cgtgccttat
     2341 gtgtccaaag ccaccggcgt gccgctggcg aagatcgccg cacgctgcat ggcggggcag
     2401 tctctggcgc agcagggcgt gacccgtgaa gtgatcccgc cttactactc ggtgaaggaa
     2461 gcggtattcc ccttcatcaa gttccccggc gtggacacca ttctcgggcc cgagatgaaa
     2521 tcgaccggcg aagtgatggg cgtgggcgag acctttgccg aagccttcgt caagtcgcaa
     2581 ctggcggcca gcgtgaagct gccgaaggac ggcaaggtat tcatcagcgt gcgcgatgcg
     2641 gacaaggccg gcgcggtcga agtggcccgc tcgctggtcg agctgggctt caccattctg
     2701 gcgacgcgcg gaacggcggt ggcgattacc gccgcgaacg tgccggtcac cgtggtcaac
     2761 aaggtggccg agggtcgtcc gcatatcgtg gacatgatca agaacggcga agtcagcctg
     2821 atcatcaaca ccgtggacag caagccttcc gcgatgcgcg attcgtattc gatccgtcac
     2881 gccgccctgc aggggcgagt gacctactac accacgctgg ccggcgcacg cgccgcctgc
     2941 gtcggtatgc aacatcttac cgatctgcaa gtgtacgatg tgcagactca acataaacga
     3001 ttggcttgaa ggaagcatta gcatgagcaa gattccattg acactcgtcg gcgcggagct
     3061 gttgcgccag gagttacata atctcaagac ggtagaacgt ccttcggtga tcaatgcgat
     3121 ctcggaagcg cgcgcgcagg gtgacctctc cgagaatgcc gagtatgaag cggccaagga
     3181 gcgccagtcg tttgtcgaag gccgtatcgt cgagctggaa ttcaagctgg gcagtgcgca
     3241 ggtgatcgat cccaagacgc tcaataccga cggtcgttgc gtgttcggta cgaccgtggt
     3301 gctggaagat gtgaacaacg gcgacgtggt gacctatcag atcgtcggcg acgacgaagc
     3361 ggatatccgc aagcgcaaga tttcgatcag ttcaccgatt gcacgtgcac tgatcggcaa
     3421 gagcagcggt gacattgccg aggtgaaggc gcccggcggc gtgcgcgaat acgaagtggt
     3481 ggaagtgcag tacgtctgaa gcggttagtg gtaagtaggg agcgggaaaa cccgactgac
     3541 tactgaggca cgaataacca gcgacttggc gagcgttttt gttcgcttag tcggatggct
     3601 agtagtttca tcagtgccgc gccactccct gctttccggt cggacgcagt ccgacctagc
     3661 ggctaccgag ttgtttcttg gtcagcggtt tcttgccctt gtggcgcggc atcttcttga
     3721 gttcgatctg attcgcttcc ggattgggca gatagatcac cagaatcttt ccgatatgct
     3781 ggaccggcgc ggcgttcagt tgagtgcaga tgtcggtcat gatggcctcg cgttgcgggc
     3841 gttccgccat gacgcggatc ttgatgagtt cgtgggcctt caggttcagt gcgatttctt
     3901 tcagcaccga ctcggtcaga ccggcgttgc ctatcatcac ggttggattc agggcgtggg
     3961 cgcggccctt cagggcgagg cgttctgcga cagtaagcga taacatgatg acactccaaa
     4021 tttaaggtac ggattctaaa cgatgaagcc ttctaaaacc agcaaacaat ggatgcgtga
     4081 gcatctgagc gacccttttg tgcaaatggc gcaaaaagag ggttaccgtt cgcgtgccgc
     4141 ctacaaatta ctggagatcg atgccaagga ccatctgct
//
LOCUS       DASUIV010000084        20317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1135454, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000084 DASUIV010000000
VERSION     DASUIV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 20317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20317
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135454"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1379)
                     /locus_tag="VFW59_03035"
     CDS             complement(69..1379)
                     /locus_tag="VFW59_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX5363218.1"
                     /translation="MSFKQVVCWAVAGTLGSLSGGASASGFALLEQNASGMGNAYAGG
                     AAVADDASTIFFNPAGLSRLSGKQLVVAAHAIRPSAKFGNTGSTAAGFQTLGGNGGDA
                     GSWVGVPNAYFAMELSPKMKAGVGLNAPFGLQTQYDANWAGRFQAIKSSIQTVNLSPS
                     FSYEITDAVSIGAGLNYQRISGELTSSTNYSAAAFSVGGAGALAAIGGAGVEGLSTMT
                     GSDSAWGYNLGALFKVGEQTRVGVAYRSRIKYNLTGTVVFANRPAALAAAIPDGPVTL
                     GVTLPESFSASVFHQIDDKWDVMADATMTGWSSFQQLKVLRTNGATVLNVPENWRDTW
                     RIAVGANHHYSEQWTSRMGLSYDQSPVPDAFRTARIPDADRFEIALGGQYKSGRNSAI
                     DFGYAHLFVNNANIADRQGLAKGNLVGSYQNSADILSIQYTQNF"
     gene            complement(1437..1844)
                     /locus_tag="VFW59_03040"
     CDS             complement(1437..1844)
                     /locus_tag="VFW59_03040"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5363219.1"
                     /translation="MDEDQLITTTLPHREPTIRVVAMPSDANYTGDVFGGWLMGQVDI
                     AGSIPALHRAKGRVATVAVNSFVFKQPIFVGDIVSFYAHIVKVGRTSITVNVEVYSQR
                     DPERPTCVKVTEATLTYVAVDEARKPRVVPEES"
     gene            complement(1846..3645)
                     /locus_tag="VFW59_03045"
     CDS             complement(1846..3645)
                     /locus_tag="VFW59_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5363220.1"
                     /translation="MQVQQQDDVITPQQAVTLHGLFLERVRRTPDKVAYRHFIDGAWR
                     DYTWREMRDEVGRWQVGLARLGLQRGDRVAIMLRNCPYWMMFDQAAMSLGLVVVPLYT
                     VDRPDNLAYIVNDADVKVMLFENAEQWRVLNTVRDQLGGVVRFVSIESIPNSEPRLVA
                     MADFLSTAARLQSAPPCRSDELASIVYTSGTTGKPKGVMLSHNNMLSNAYATLGILAV
                     HGDDLFLSFLPLSHTFERTLGYYLPVMAGATVAFARSIPLLSEDLKIIRPTLLISVPR
                     IYERIHAAIRTKLDESSALKRKLFHLAVKVGWARFEHAQGRGPWMVSFLLWPVLQKWV
                     AQKILDRMGGRLRAAISGGAALSPEISHMFVGLGLPVLQGYGLTETSPVISGNHLENN
                     FPDSVGRPTPDLQVRLGEQRELLVKGPNVMMGYWNNPEATRAIIDADGWLNTGDVARI
                     SETGHIYLTGRTKEIIVMSNGEKIPPSDMEIAILHDPLFDQVMIFGEAHPYLVALAVV
                     NPDGWQHFAQEIGIRADMPEALSDSRVETRVLKRIARNLRGFPGYAKVNRVLLLREPW
                     TIENGLLTPTLKIKRADVVQRYAAQIKELYEGH"
     gene            complement(3657..4490)
                     /locus_tag="VFW59_03050"
     CDS             complement(3657..4490)
                     /locus_tag="VFW59_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5363221.1"
                     /translation="MSSLRNKVILVTGASRGIGREIALRCARDGAKVVITGKTIAPHP
                     KLPGTIHTVAAEVEAAGGQALALQLDVRDEQAIHDAVARTVEYFGAIDVLVNNASAIS
                     LSGTLDTPAKKLDLMWDVNMRATFLMSQACIPYLEQADNPHILNLSPPLNMDAQWFAP
                     HLAYTLSKYGMSMCALGMAREFAAEGKNGIGVNCLWPRTSIATAAIEFNFPAEVLRAS
                     RKPAIVADAAWQILTRHARDCSGNFFIDEEVLREAGVTDFGGYAVSPGAVLHRDLFLD
                     E"
     gene            complement(4487..6925)
                     /locus_tag="VFW59_03055"
     CDS             complement(4487..6925)
                     /locus_tag="VFW59_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5363222.1"
                     /translation="MLLLTILLALAILTILAFFRASTVSWILAMMVIVPVIAIQSRIS
                     DTALQGVYVLLILFIAIFGIASLRRAVFSGNVLKLFRKILPPISATEQEAIDAGTVWW
                     DGELFSGDPDWDGLLEFPQCALSEEEQSFLDNEVEQLCAMLNDWDITHNRADLPPEVW
                     QFLKIKGFFGMIIPKQYGGREFSAQAHSAVVAKVASRSGSAAVTVMVPNSLGPAELLL
                     HYGTDEQKEKYLRRLAYGLEVPCFALTGPFAGSDAGAIPDDGVVCRGDFDGQKDVLGI
                     RLNWEKRYITLAPVATLLGLAFKLHDPERLLGGEVERGITLALIPTNLPGVKIGRRHA
                     PLNSAFLNGPTQGRDVFIPMEYIIGGTECVGQGWRMLMECLAAGRAISLPASSVGVMK
                     QAARTSGAYARVRKQFKLPIGRFEGVEEPLARIGAHTYMVDAARQFTALAVDMGEKPS
                     VISAVIKYHATERGRIVINDAMDVHGGKGICMGPDNYLARNYQQAPIGITVEGANILT
                     RSMIIFGQGAIRAHPYVLKEIAAAHEPDRDLAQHQFDEALFAHISFAMSNAARSLVFG
                     VTGGIGIYAPPGAETRRYYQQLTRFSAAFALASDVAMGVLGGSLKRHEKLSGRLGDVL
                     SLMYLCSATLKRFEDDGRPDADLPLLHWAMQDAIYRMQQALDGVVQNFPHAAIRWLLG
                     ALIFPVGLRLSPPSDQLGHEIATLLMQPGEARDRLTAGIYVSGDEQDAVGALEAALTS
                     TLLCEPLQAELEAARKAGKLQARDELARIDEARDIGLIDAEQAALLERDYGLRRKAIM
                     VDDFSPAELRAWHA"
     gene            complement(7027..8385)
                     /gene="radA"
                     /locus_tag="VFW59_03060"
     CDS             complement(7027..8385)
                     /gene="radA"
                     /locus_tag="VFW59_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5363223.1"
                     /translation="MAKAKTIYSCTECGGQTPKWQGQCPHCMEWNTLVESVAETVTKG
                     TNRFSALAASGKVQMLSEVEAAEVPRTPTGIEEFDRVLGGGLVSGGVVLIGGDPGIGK
                     STLLLQVLAHLSKQQKTLYVSGEESAQQIALRAKRLSLDAQGLRLLPEIQLEKIQAAI
                     AADKPDVVVIDSIQTVYSEQLTSAPGSVAQVRECAAQLTRIAKSQNITIILVGHVTKE
                     GALAGPRVLEHIVDTVLYFEGDTHSSFRLIRAFKNRFGAVNELGVFAMTEKGLREVSN
                     PSALFLSQHGTQVAGSCVMVTQEGTRPLLVEIQALLDEAHSPNVRRLSLGLEQNRLAM
                     LLAVLHRHAGIACFDQDVFINAVGGVKITEPGADLAVILAMVSSLRNKPLPEKLVVFG
                     EVGLAGEVRPVQRGQERLKEAAKLGFTHAVIPKANAPKHNIPGMEIIAVERVEQAVER
                     MR"
     gene            complement(8506..9066)
                     /locus_tag="VFW59_03065"
     CDS             complement(8506..9066)
                     /locus_tag="VFW59_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HEX5363224.1"
                     /translation="MMQTKFKAALLVAGLLASGAALAEESFFQWASSISREKEVKPVD
                     NQIYKEECGACHFAYPPGLLPGKSWEKLLTETALSEHFGDDASLDADSLKEIRDYALE
                     NAADKSYYKRSRKIALATREGDAPLRITEVRYIKRKHHDIPEKMIKGNKDVKSLSFCN
                     ACHTKAGEGNFDNDTVKIPNYPDWKD"
     gene            complement(9078..9482)
                     /locus_tag="VFW59_03070"
     CDS             complement(9078..9482)
                     /locus_tag="VFW59_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HEX5363225.1"
                     /translation="MKKRYTSMALLGALFIASTAGATPATDELFARYKADGASNFDGE
                     RGAKNWNKKVKGEDGETMSCATCHGDDLSKTGKHHKTSKVIQPMAPSANAERFTEVKK
                     IEKWFKRNCNDAWGRECTPQEKGDFLKFLLSK"
     gene            complement(9497..10114)
                     /locus_tag="VFW59_03075"
     CDS             complement(9497..10114)
                     /locus_tag="VFW59_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363226.1"
                     /translation="MEMRAVKFIGVAVLCLAALSDRAGADELPTLSDAYVRDYESFKL
                     AASDSEVLVKPEPATEIPAPKAAEFEPPMFTGSKMHQYLGIATVTAAALTFATHFHPC
                     EGPTCGPQPPRQTNGTHAKLGKATALLAAATVASGLISHWDDFHKEDGISDPDNLHVL
                     LGVTGAALMAYAINKSANSSTTPTSHAAMAELGALSMIVAIKLTW"
     gene            complement(10095..10727)
                     /locus_tag="VFW59_03080"
     CDS             complement(10095..10727)
                     /locus_tag="VFW59_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5363227.1"
                     /translation="MQQTDKNRIEGGKTVKVWDLFIRFFHWTLVLGFATAYVSGEYHY
                     SEIHTLTGYALCLLVLARVYWGFKGSQYARFRSFIFSIGETRAYLRSMIAGHPRHYFG
                     HNPAGALMVFALLATVASLLVTGLMTLSVIDFEGPLLFLANLVSDETSYAIRSLHEFL
                     PTVGLVLVALHLMGVVSGSIQHKENLVRAMVTGKKELPSNQNEGHGNEGC"
     gene            complement(10925..11383)
                     /locus_tag="VFW59_03085"
     CDS             complement(10925..11383)
                     /locus_tag="VFW59_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEX5363228.1"
                     /translation="MQIWVDADACPKVIKEILFRAAERRQIPLTLVANKLLHCPPSGV
                     IRAMQVPAGFDVADSRIVELTEAGDLVITADIPLAALVIAKGGHALNPRGELYTQDTI
                     QERLTMRNFMENLRSSGVETGGPSTLSQSDRQAFANQLDKFLLKQAKTGA"
     gene            complement(11443..12630)
                     /locus_tag="VFW59_03090"
     CDS             complement(11443..12630)
                     /locus_tag="VFW59_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5363229.1"
                     /translation="MPKIAKDALLQIPRGVWVLGFVSLLMDVSSEMIHSLLPLFMVTV
                     LGASALTVGVIEGIAESTALLVKVFSGALSDYLGRRKGLALFGYALGAFTKPMFALAS
                     SMGMVLGARFLDRVGKGVRGAPRDALIADITPAAIRGAAFGLRQSLDTVGAFLGPLLA
                     VGLMLLWANDFRAVFWVAVIPGLLAVALLLFGVHEPERTQAHQPVNPVSRENLRRLGA
                     AYWWVVGIGAVFTLARFSEAFLVLRAAQGGIAVALVPLVMVAMNVIYAASAYPFGKLS
                     DRMSHTKLLALGLAVLIAADLVLATDDHWVTVLAGVALWGIHMGITQGLLARMVADVT
                     PGDLRGTAYGFFNLMSGLAMLLASVLAGLLWDRLGAAFTFYAGAAICAIAAIGLLWRP
                     VGR"
     gene            complement(12614..13015)
                     /locus_tag="VFW59_03095"
     CDS             complement(12614..13015)
                     /locus_tag="VFW59_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363230.1"
                     /translation="MKPLLAWFVLLAAAVINGGLRDFTYGRQVSELLAHQISCVSGIA
                     LFAVVIHQYVRRWPPVSAREAWYIGLFWMLLTVAFEFLFFHYVAGHPWPVLLANYDLS
                     EGRLWPLILLWVLVAPYFFYRLSGKKHAQDR"
     gene            complement(13012..13548)
                     /locus_tag="VFW59_03100"
     CDS             complement(13012..13548)
                     /locus_tag="VFW59_03100"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5363231.1"
                     /translation="MTKKSFPLSKVYRLLEPGPVLLVTTAHKGRANIMTQSWHTMMEF
                     EPPLVGCVISGRNFSFSALKKTKECVLSIPTVELAKQVIGVGNCSGERVDKFKKFKLT
                     QQPASMVAAPLIAECYANLECRVVDTRMVNKYNFFVLEVVKAWIAPDRKNPRTLHHQG
                     KGVFRVSGDTIKLPSKMK"
     gene            complement(13721..14014)
                     /locus_tag="VFW59_03105"
     CDS             complement(13721..14014)
                     /locus_tag="VFW59_03105"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5363232.1"
                     /translation="MQQRVKKQLEWSVRSGHDLLAIDAHISSDNSVAAEQVTDYLIAQ
                     AELLTRFPLLGRIPKPGAPRELVFTRYPYNLIYRVTRTKIRIVRVIHQSRLYP"
     gene            complement(13986..14234)
                     /locus_tag="VFW59_03110"
     CDS             complement(13986..14234)
                     /locus_tag="VFW59_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363233.1"
                     /translation="MLNELKLPRTLAKRLEKVAAVAHLNPESILKTALADRLDYLEWK
                     EKAIAEGQADLDNGNVVTTAQIRESLAKQRATKSKKAA"
     gene            14413..16350
                     /locus_tag="VFW59_03115"
     CDS             14413..16350
                     /locus_tag="VFW59_03115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX5363234.1"
                     /translation="MSIEVERFFSEQSPLVTEVASFRPRAQQREMALAVAEAIRDNAI
                     LVAEAGTGTGKTFAYLVPALLNGGKVIISTGTKNLQDQLFQKDLPMVRDALRAPVSVA
                     LLKGRSNYVCHYHLARAESDGQFKTREDVRHLGKIVKYAKVSDSGDKSGLADVPENAP
                     IWMQVTSTRDNCLGQECPNHKECFVLKARTEAMKADIVVVNHHLFFADVMLRDEGVAE
                     LLPACNTVIFDEAHQLPETASLFFGENISTSHLLELAIDSRIEAAASAKDFAPLPKAC
                     DELDKAARDLRLVFKKEGRMPAIATQDFREFAPSLKVLGEKLTQLSGLLEKQAERSEG
                     LENCWQRAQALSQQLAKWQKDEEPDQVRWLEIFHHSLQLNTTPLSIAEIFEKQIGGNA
                     RAWIFTSATLAVKQDFSHYLGEMGLAKARTACWDSPFDYGKQGLLYVPQNLPEPNSEG
                     YTEAVVQAALPLIEASRGRAFLLFTSLRAMQRAHEILLAEFDRRGWTYPLLLQGEGSR
                     NELLSRFRDHGNAVLLGSQSFWEGVDVRGEALSLVIIDKLPFAPPDDPVLAARLDQLK
                     KQGRNAFMEYQLPRAIITLKQGAGRLIRDENDRGVLMICDPRIITKQYGKRVWQSLPP
                     MKRTRDEAVAVAFFTTPAVQD"
     gene            complement(16347..16544)
                     /locus_tag="VFW59_03120"
     CDS             complement(16347..16544)
                     /locus_tag="VFW59_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HEX5363235.1"
                     /translation="MNDSNSPVETEKVACAVCRKEIPLSEAKTPEAVADYVAHFCGLD
                     CYEQWKQQSVKAGHRGDKTGS"
     gene            16753..17208
                     /locus_tag="VFW59_03125"
     CDS             16753..17208
                     /locus_tag="VFW59_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX5363236.1"
                     /translation="MNNKLKNLMIAAGIIASFSLNALAADATPAPSMPDHAQHLERMK
                     TLTPEQRAKEQEEMQQKMQSLTPEQRAEHRKAMREQWEKMSPEDRKAMHDAMHEDMQG
                     MSPDECKVRHEAMDKNGMTPQQFGHGNGQHCNVKPPKGPCPYDKTKPTK"
     gene            17257..18162
                     /locus_tag="VFW59_03130"
     CDS             17257..18162
                     /locus_tag="VFW59_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5363237.1"
                     /translation="MNEAMLKLVHGRNDLYPHALEKKFLRIFNRIVELWDTPQMDAYF
                     TELMMDTRGGSRQGFPKEVVAEIYQLSRIHERSQQRAPVQPSDPWAEIEIGKRRAIEE
                     LGYECTPHDFLKAVESQNKKALAAYLSAGLPVDTRDERGWTPLMISAFNGNEEMAELL
                     IRSGADVQIEDHAGYGPMHWAAFNGYRRVVRLLSSQRGNVNARSHHGWTPLLQAATRG
                     HLDVCIALISSGANVNLTSNDGWSPLHKACANGHIEVVQLLLSQGADRNAHYQDSVTP
                     LALAIKNKREDIVALLTSGVIADKR"
     gene            complement(18159..19478)
                     /gene="rimO"
                     /locus_tag="VFW59_03135"
     CDS             complement(18159..19478)
                     /gene="rimO"
                     /locus_tag="VFW59_03135"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX5363238.1"
                     /translation="MQKHTPPKVGFVSLGCPKATSDSEQILTRLRAEGYLISGSYQDA
                     DLVVVNTCGFIDSAVEESLDAIGEALAENGRVIVTGCLGAKGDVVQQAHPKVLAVTGP
                     HAADEVMEAVHQHLPKPHDPLTDLVPAQGIRLTPRHFAYVKISEGCNHSCTFCIIPSL
                     RGDLVSRPVGEVMQEARHLVDAGVKELLVISQDTSAYGVDVKYRTGFWGGRPLRTRMT
                     ELAVALGELGVWVRLHYVYPYPHVDEVIPLMAEGKILPYLDIPFQHASPRILKLMKRP
                     GNSDGVLERIKRWREICPELTLRSTFIVGFPGETEAEFEELLEFLKEAQLDRVGAFAY
                     SPVEGAAANELPDHVPPEVQQERLERLMLLQEQISTERLKRKIGKTITVLVDEVDEEG
                     AVARSSADAPEIDGLVYIEDGQQLQVGDLVEVKVTDSDEHDLWAELV"
     gene            complement(19518..20297)
                     /locus_tag="VFW59_03140"
     CDS             complement(19518..20297)
                     /locus_tag="VFW59_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5363239.1"
                     /translation="MTILLWIVIASIAGGVLSVLGAALFAFNSHMQRYLGAMVSYAIG
                     ALLGAVFLDILPEAITLSTNVAALSATVLFGIMLFFILEKLVLWRHCHHEHCEAHESP
                     AVAHSHDHGRTGLMITVGDTFHNFVDGIIIAAAFLTDVHLGMVTALAIIAHEIPQEVG
                     DFVILLHSGYSKLRAFQLNLISSFASVAGGVLGYFTLQTMEAWIPTLLALAAASMLYI
                     AVADLIPGLHKRTRLRDTLQQVLLIGSGVATIYIMSLFVQA"
BASE COUNT         4093 a         6672 c         5873 g         3679 t
ORIGIN      
        1 aaaaattttc ggacatcgga ctctccaaat gaacagggag ggtaatcacc ctccccgcaa
       61 ggtacgactc agaagttttg cgtgtactgg atgctcagga tatccgcgct gttctgatag
      121 ctgccgacca ggttgccctt ggcgagcccc tgacggtccg cgatattggc gttattgacg
      181 aacaggtggg catagccgaa atcgatggcg ctgttgcggc cagacttgta ctgcccgccc
      241 agcgcgatct cgaaacggtc cgcatccggg atgcgcgcgg tgcggaacgc atcgggcaca
      301 gggctttggt cataggaaag tcccatccgc gaagtccact gctcgctgta gtgatggtta
      361 gcgcctaccg cgatacgcca ggtgtcgcgc cagttctccg gcacgttcag caccgtggcg
      421 ccgttggtgc gcagcacctt gagttgctgg aacgagctcc agccggtcat ggtcgcatcg
      481 gccatcacgt cccacttgtc gtcgatctgg tggaacacgc tggccgaaaa ggattccggc
      541 agcgtgacgc ccagcgtgac cgggccatcc gggatggcgg cggcaagcgc ggcgggacgg
      601 ttggcaaaga caaccgtccc ggtcaggttg tatttgatcc gcgagcgata ggcaacacca
      661 acgcgcgtct gctcgccgac cttgaacagt gcacccaggt tatagcccca tgcactgtcg
      721 ctgccggtca tggtgctcag cccttccacc ccggcaccgc cgatagctgc cagcgcgccg
      781 gctccgccca ccgaaaacgc cgccgcactg taattggtgg agctggtcag ctcgccgctg
      841 attcgctggt aattcagacc cgcgccgatg ctcaccgcat cggtgatttc ataagaaaac
      901 gaggggctca gattaacggt ttgaatactg gatttgatcg cctgaaaacg ccccgcccag
      961 ttcgcgtcgt actgagtctg cagaccgaac ggcgcattca ggccaacgcc tgccttcatc
     1021 ttcggactga gctccatcgc gaaataggca ttgggcaccc ccacccaact acccgcatcg
     1081 ccgccgtttc cgccgagggt ctgaaacccg gccgcagtcg agcccgtgtt gccgaacttc
     1141 gccgaggggc gaatcgcatg tgccgccacg accagctgct tgccggaaag acgcgagagc
     1201 ccagccggat tgaagaagat ggtggaggcg tcatccgcca cggccgcacc gcccgcatag
     1261 gcgttgccca tgccgcttgc gttctgctcc agcaacgcga accccgaagc cgatgcgcct
     1321 ccggacagtg accccaaagt gcccgcaacc gcccagcaca ctacttgttt gaacgacatg
     1381 attctctctc cgattgatat taacgagccc ggcccaagaa tcgccgggcg atacacttac
     1441 gattcttcgg gtacgacacg cggcttgcgc gcctcgtcca ccgccacata ggtcagcgtc
     1501 gcctcggtga ccttgacgca ggtcggacgc tccgggtcgc gctgcgaata cacctcgacg
     1561 ttgacggtga tcgaggtgcg cccgaccttg acgatatgtg catagaaact cacgatgtcg
     1621 ccgacgaaaa tgggttgctt gaacacgaag gagttcaccg ccaccgtcgc cacccggcct
     1681 ttggcccggt gcagcgccgg gatgctgccg gcgatgtcca cctgccccat cagccagccg
     1741 ccgaacacgt cgcccgtgta attggcatcg gaaggcatcg ccaccacgcg tatggtgggc
     1801 tcgcggtgcg gcaaagtggt cgtgatcagc tggtcttcat ccatcctagt gcccctcgta
     1861 gagttccttg atctgtgcgg cataacgctg caccacatcg gcgcgcttga tcttcagcgt
     1921 cggtgtcagc aggccgttct cgatcgtcca tggctcgcgc aacagcagca cgcggttcac
     1981 cttggcatag ccgggaaaac cgcgcaggtt gcgcgcgatg cgcttcaaca cccgggtctc
     2041 gacccggctg tcgctcaacg cctcgggcat gtcggcgcgt atgccgatct cctgggcgaa
     2101 atgctgccag ccatccgggt tgaccacggc cagcgcgacc agatagggat gcgcctcgcc
     2161 gaatatcatc acctgatcga acaggggatc atgcaggatg gcgatctcca tgtcggaggg
     2221 cggtatcttc tcgccgttcg acatcacgat gatctccttg gtgcgcccgg tgaggtagat
     2281 atgccccgtc tccgagatac gcgccacatc gccggtattc aaccagccgt cggcatcgat
     2341 tatcgcccgc gtcgcctcgg ggttgttcca gtagcccatc atcacgttcg gccccttcac
     2401 cagcagttcc ctctgctcgc ccaggcgcac ctgaaggtca ggggtaggcc gcccgacgct
     2461 atccggaaaa ttgttctcca gatggttacc cgaaatcacc gggctggtct cggtcagacc
     2521 ataaccctgt aacaccggca accccaggcc aacgaacatg tgcgatattt ccggcgacag
     2581 tgcagccccg ccgctgatcg ccgcacgcag cctgcccccc atgcggtcga ggatcttctg
     2641 cgccacccac ttttgcagca ccggccacaa caggaaagaa accatccacg gcccgcgccc
     2701 ctgcgcatgt tcgaaacgag cccagccgac cttgaccgcc agatggaaca gcttgcgctt
     2761 gagcgccgac gactcgtcaa gcttggtgcg tatcgccgca tggatgcgct cgtagatacg
     2821 cggcaccgag atcagcagcg tcggacggat gatcttcagg tcttccgata gcagcgggat
     2881 cgagcgcgcg aacgccacgg tggcacccgc catcaccggc aagtaatagc ccagcgtgcg
     2941 ctcgaaggtg tgcgacagcg gcaggaacga caggaacaga tcgtcgccat gcacagcgag
     3001 tatccccagc gtcgcatagg cgttgctcag catgttgttg tgactgagca tcacgccctt
     3061 gggtttcccg gtggtacccg aggtgtagac gatactcgcc agctcgtcgc tgcggcatgg
     3121 cggtgcagac tgcaaacgcg ccgctgtcga caggaaatcg gccatcgcaa ccagtcgcgg
     3181 ctcgctgttc ggtatcgatt cgatgctgac gaagcgcacg acgccgccca gctggtcgcg
     3241 cacggtgttc agcacccgcc actgttcggc attctcgaac agcatcacct tcacgtcggc
     3301 atcgttgacg atgtaggcga ggttatccgg acgatccacg gtgtacagcg gcaccaccac
     3361 cagccccagc gacatcgcgg cctgatcgaa catcatccag taaggacaat tgcgcagcat
     3421 gatcgccacg cggtcgccgc gctgcaatcc gagtctcgcc agccccacct gccagcgccc
     3481 cacctcgtcg cgcatctcgc gccaggtgta gtcgcgccac gcgccatcga tgaaatgacg
     3541 ataggcaacc ttgtcaggcg tgcggcgcac acgttcgaga aacaatccgt gcaaggtcac
     3601 cgcctgctgc ggtgtgatca catcatcttg ttgttgcacc tgcatcctat tccctgtcat
     3661 tcatccaaaa ataaatcgcg gtgcaagacc gcgcccggcg aaaccgcata gcccccgaaa
     3721 tcggtcacgc ccgcctcgcg cagcacctct tcgtcgatga agaagttgcc gctgcagtcg
     3781 cgggcatgac gcgtcagaat ctgccaggcc gcatcggcca cgatggcagg cttgcgcgag
     3841 gcgcgcagca cttctgcggg aaagttgaat tcgatcgcgg cggtcgcaat gctggtgcgc
     3901 ggccacagac aattgacccc gatcccattt ttaccctccg ccgcaaactc gcgcgccatt
     3961 cccagcgcgc acatgctcat gccgtacttc gacagggtat aggccagatg cggcgcaaac
     4021 cactgcgcat ccatgttgag cggcggcgac agattgagga tatgcggatt gtccgcctgc
     4081 tcgagataag ggatacaggc ctgcgacatc agaaaggtgg cgcgcatgtt cacatcccac
     4141 atcagatcga gctttttcgc gggggtgtcc agcgtgccgc tcaggctgat cgcactggcg
     4201 ttattcacca gcacgtcgat cgcaccgaaa tattccaccg tacgcgcgac cgcatcatgg
     4261 atcgcctgtt cgtcgcgcac gtcgagctgt agcgccagcg cctgcccgcc cgccgcctcc
     4321 acttcggcgg ccactgtgtg tatcgtgccc ggcaacttgg gatgcggcgc gatagtcttg
     4381 ccggtgatga ccaccttcgc gccgtcgcgt gcgcagcgca acgcgatctc ccgtccgatg
     4441 ccgcgcgagg caccggtgac aagaatcacc ttgttgcgca aggaactcat gcgtgccagg
     4501 cgcgcaattc cgcaggagag aagtcatcca ccatgatggc cttgcggcgc agcccgtagt
     4561 cacgctccag cagcgcggcc tgctccgcat cgatcagacc gatgtcgcgc gcctcgtcga
     4621 tgcgcgccaa ttcatcccgc gcctgcaact tgccggcctt gcgcgccgcc tccagttcag
     4681 cctgcaacgg ttcgcacagc aaggtgctgg tcaacgccgc ctccagcgca ccgaccgcat
     4741 cctgctcgtc gccggataca tagattcctg cggtcagacg gtcgcgcgcc tcgcccggct
     4801 gcatcagcag ggtagcaatc tcgtgtccga gttgatcgga tggcggcgac aggcgcagcc
     4861 ccaccgggaa gatcagcgcc cccagcaacc agcgtatcgc ggcatggggg aagttctgca
     4921 ccacgccgtc cagcgcctgc tgcatgcggt agatcgcatc ctgcatcgcc cagtgcaaca
     4981 gcggcaggtc ggcatcggga cggccgtcat cctcgaaacg tttcagcgtc gcggagcaca
     5041 ggtacatcag gctcagcaca tcgcccagcc gcccggacag cttctcgtgc cgcttcagcg
     5101 agccgcccag cacccccatc gccacgtcgg aggccagcgc gaaggcggcg gagaagcgcg
     5161 tcagctgctg ataatagcgg cgcgtctcgg cccccggcgg tgcgtagatg ccgatcccgc
     5221 cggtgacacc gaacaccaga ctgcgcgccg cgttgctcat cgcgaagctg atgtgggcga
     5281 acaacgcctc atcgaactgg tgttgcgcca gatcccggtc cggctcatgg gctgccgcga
     5341 tttccttcag cacataggga tgggcgcgga tcgcgccctg accgaagatg atcatgctgc
     5401 gcgtcaggat gttcgcgcct tcgaccgtaa tgccgatcgg cgcctgctgg taattgcgcg
     5461 ccagataatt gtccggcccc atgcagatgc ccttgccgcc atgcacatcc atcgcgtcgt
     5521 tgatgacgat gcggccgcgc tcggtcgcgt gatacttgat gacggcagaa atcaccgacg
     5581 gcttctcgcc catgtcgacc gccaatgcgg tgaactggcg cgccgcatcc accatgtagg
     5641 tgtgcgcacc gatgcgtgcc agcggctcct ccacgccctc gaagcggccg ataggcaact
     5701 tgaactgttt gcgcacgcgt gcgtaagcgc cgctggtacg ggcagcctgc ttcatcacgc
     5761 cgacgctgct ggcaggcaac gaaatcgcac gccccgccgc cagacattcc atcagcatgc
     5821 gccagccctg gcccacgcac tccgtgccgc cgatgatgta ctccatcgga atgaacacgt
     5881 cacggccctg cgtcgggccg ttcaggaagg ccgaattcag cggtgcatgg cggcgcccga
     5941 tcttcacacc gggcaaattg gtcggaatca gcgccagcgt aatgccgcgc tcgacctcgc
     6001 cgcccagcaa gcgctcgggg tcgtgcaact tgaacgccag accgagcagc gtcgccaccg
     6061 gcgccagcgt gatatagcgc ttctcccagt tcaggcggat gcccagcaca tccttctgac
     6121 cgtcgaaatc gccacggcac accacgccat catcggggat ggcgccggca tcggaaccgg
     6181 cgaatgggcc ggtgagtgca aaacacggca cctccagtcc ataggccaga cggcgcagat
     6241 atttttcctt ctgctcgtcc gtgccgtaat gcagcagcaa ctcggcgggc cccagcgaat
     6301 tcggcaccat caccgtcacc gccgccgaac cgctgcgcga agcgactttc gccacgaccg
     6361 cagaatgcgc ctgcgcggag aactcgcgcc cgccgtactg cttcgggatg atcatgccga
     6421 agaagccctt gatcttgagg aactgccaga cttcgggcgg cagatcggcg cggttatggg
     6481 tgatatccca atcgttcagc atggcgcaca gttgctcgac ctcgttatcg agaaagctct
     6541 gctcctcctc gctcagcgcg cactggggaa actccagcag cccatcccag tccggatcgc
     6601 cgctgaacag ttcgccatcc caccacaccg tgcctgcatc gatcgcctcc tgctcggtgg
     6661 cggagatcgg cggcagtatc ttgcggaaca atttgaggac attgccgctg aacacggccc
     6721 ggcgtaacga ggcaataccg aagatcgcga tgaacaggat caacaggacg tagaccccct
     6781 gcaaggccgt gtccgagatg cgcgactgga tcgcgatcac cggcacgatc accatcatcg
     6841 cgagtatcca gctgacggtg gaggcgcgga aaaatgccag gatagtgagg atggccaatg
     6901 ccagcaggat ggtcaacaac aacatattcg aactccctcg atttattgtt tttcttgatg
     6961 cgtcaccgca ctttaacgcg atacatcaaa ccgcacaaca acgcaggggc acggcgtgcc
     7021 gtgcccctac cgcatccgct ccaccgcctg ctcgacgcgc tccaccgcga tgatctccat
     7081 gccgggaata ttatgcttgg gcgcattggc cttggggatg acggcatggg tgaaaccgag
     7141 cttggcggct tccttcaggc gctcctgtcc gcgctgcacc ggacgcacct cgcccgccag
     7201 cccgacctcg ccgaacacca ccagcttttc cggcagcggc ttgttgcgca ggctggagac
     7261 catcgcgagg atcaccgcca gatcggcgcc cggctcggtg atcttcacgc cgcccaccgc
     7321 attgatgaac acatcctgat cgaagcaggc gatgcccgca tggcggtgca gcaccgccag
     7381 cagcatcgcc agccggttct gctccagccc cagcgacagc cgccgcacgt tgggcgagtg
     7441 cgcctcgtcg agcagcgcct ggatctccac cagcagcggt cttgtgcctt cctgcgtcac
     7501 catcacgcag gaacccgcga cctgcgtacc gtgctgcgac aggaacagcg ccgacgggtt
     7561 gctgacttcg cgcaggccct tctcggtcat cgcgaacacg ccgagttcgt tcaccgcacc
     7621 gaagcggttc ttgaacgcgc ggatcaggcg gaagctggaa tgggtgtcgc cctcgaaata
     7681 cagcaccgtg tcgacgatgt gctccagcac gcgcggcccg gccagcgccc cttccttggt
     7741 gacgtgaccg accagaatga tggtgatgtt ctggctcttg gcgatgcggg tgagctgcgc
     7801 ggcgcattcg cgcacctgcg cgaccgagcc gggcgcggaa gtcagctgct cggaatacac
     7861 cgtctggatc gagtcgatca ccaccacgtc gggcttgtct gcggcgatgg ccgcctgaat
     7921 cttttccagc tggatctccg gcagcaggcg caggccctgc gcatccagcg acagccgctt
     7981 ggcgcgcagc gcgatctgct gcgcggattc ctcgccgctg acatacagcg tcttctgttg
     8041 tttggacaga tgcgccagca cctgcagcag cagcgtggac ttgccgatgc ccggatcgcc
     8101 gccgatcagc accacgccgc ccgacaccag cccgccgccc agcaccctgt cgaattcctc
     8161 gatgccggtc ggagtgcgcg gcacttccgc cgcctcgacc tcggacagca tctgaacctt
     8221 gccgctcgcg gccagcgcgc tgaaacggtt ggtgcccttg gtcaccgtct cggccaccga
     8281 ttcgaccagc gtgttccact ccatgcagtg cgggcactgc ccctgccact tgggcgtctg
     8341 cccgccgcac tcggtgcagc tataaatcgt ttttgccttg gccatgacca cctctgaaac
     8401 taaggctgca aggataacgc agttgcccat accagagtag caagcaggcc tgtatcgcaa
     8461 ataaaaaggg cagcccgggc tgcccttgat gcggagatac gccggtcaat ctttccagtc
     8521 cggataattc gggatcttga cggtgtcgtt atcgaagttc ccctctcccg ccttggtgtg
     8581 acaggcattg cagaaactca acgatttcac gtccttgttg cccttgatca tcttctccgg
     8641 gatatcgtga tgcttgcgct tgatgtaacg cacctcggtg atgcgcaacg gcgcatcgcc
     8701 ctcacgcgtc gccagcgcga tcttgcgcga acgcttgtag taagacttgt ccgccgcatt
     8761 ctcgagcgcg taatcgcgga tctctttcag cgaatcggca tccagcgatg cgtcgtcgcc
     8821 gaaatgttcg ctcagcgccg tctcggtcag cagcttttcc catgacttgc ccggcaacag
     8881 gccgggcgga taggcgaaat ggcaggcccc gcactcctcc ttgtaaatct ggttgtcgac
     8941 cggtttcact tccttctcgc gcgatatact gctcgcccac tgaaagaagc tttcctcggc
     9001 cagcgcagcg ccgcttgcca acagtcctgc caccagcagg gccgccttga attttgtttg
     9061 catcatgttt gccaccctta tttgctcaac aggaacttga ggaaatcgcc cttttcctgc
     9121 ggcgtgcatt cgcggcccca tgcgtcgttg caattgcgct tgaaccactt ctcgatcttc
     9181 ttcacctcgg tgaaacgctc ggcattcgca ctcggcgcca tcggctggat gaccttgctg
     9241 gtcttgtgat gcttgccagt cttgctcagg tcgtcgccgt ggcatgtggc gcaggacatc
     9301 gtctcgccgt cttcgccctt gaccttcttg ttccagttct tggcgccgcg ctcgccgtcg
     9361 aagttgctcg cgccgtcggc tttgtaacgc gcgaacaact cgtcggtcgc cggtgtcgcc
     9421 ccggcggtcg atgcgatgaa caacgcgcca agcaaagcca tcgatgtgta ccgttttttc
     9481 atgttcaagc tctccattac caggtcaatt tgattgcaac gatcatgctc agcgcgccca
     9541 gctccgccat cgccgcatgg ctggtcggtg tcgtcgaaga attcgcggat ttgttgatcg
     9601 cataggccat cagggccgcg ccggtcacgc ccagcagcac gtgcagatta tcgggatcgg
     9661 agataccgtc ttccttatga aaatcgtccc agtgcgaaat cagaccggag gcgaccgtcg
     9721 ccgctgccag cagcgccgtc gccttgccta actttgcgtg cgtgccattg gtctggcgcg
     9781 gtggctgggg cccgcaggtc ggaccttcgc agggatggaa atgggtggcg aaagtcagcg
     9841 cggcggccgt cacggtcgca atgcccagat actggtgcat cttgctgccg gtaaacatcg
     9901 gcggctcgaa ctcggccgct ttcggcgccg gaatttcagt cgcaggctct ggcttgacca
     9961 gcacctcgct gtcgctcgcg gccagcttga acgactcata gtccctgaca taggcgtctg
    10021 acagggtcgg cagctcgtcc gccccggccc ggtccgacag cgccgccaga cacagaacgg
    10081 cgaccccgat aaatttaaca gccctcattt ccatgtccct cgttttgatt gcttggcaat
    10141 tctttcttgc cggtgaccat cgcccgcacc agattctcct tgtgctggat gctgccggac
    10201 accacgccca tcaggtgcaa cgcgaccagc accagcccga ccgtaggcag gaactcgtgc
    10261 agcgaacgga tggcatagct ggtctcgtcg ctcaccaggt tcgccagaaa cagcagcggc
    10321 ccttcgaagt cgatcaccga cagggtcatc aacccggtca ccagcagcga cgccaccgtc
    10381 gccagcaggg cgaacaccat cagcgccccc gccggattat ggccaaagta atgtctgggg
    10441 tggcccgcga tcatcgaacg cagataggct cgcgtctcgc cgatggaaaa gatgaaggaa
    10501 cggaaacggg cgtactggct gcccttgaat ccccagtaca ctcgcgccag caccagcaga
    10561 cacagcgcat aaccggtcag cgtatgaatc tcggagtaat ggtattcccc cgaaacatag
    10621 gccgtcgcga accccagcac cagcgtccaa tggaaaaagc ggatgaacag atcccagacc
    10681 ttcacggtct tgccaccctc gatgcggttc ttgtcagtct gctgcatgta gcacccaaat
    10741 taacgatcca gcgacgcaca tgactccggc caggcgcatc acaaacacgc ttgtgaccat
    10801 gccaaaaaaa ttagttcgat aagccggaca gattattccg cccgacagag gtcgcgcatt
    10861 ctacccggaa atttgcgctt ttcttcaaac cggcagcggc gcacggcccg ccgctgggtg
    10921 acggtcaggc cccggtttta gcctgcttca gcagaaactt gtcgagctga ttcgcaaagg
    10981 cctgacggtc gctctggctg agcgtcgacg gcccgccagt ctccacgccg ctgctgcgca
    11041 agttctccat gaagttgcgc atggtcagcc gctcctgaat ggtgtcttgg gtatacagct
    11101 cgccgcgcgg attcagcgca tggccgccct tcgcgatcac caaggctgcc agcggaatat
    11161 cggcggtgat caccagatcg cccgcctcgg tcaattcgac gatcctgctg tccgccacat
    11221 cgaaccccgc cggcacctgc atcgccctta tcacgccgga gggagggcaa tgcagcagct
    11281 tgttcgccac cagcgtcagc gggatctggc gccgctccgc cgcacggaac aggatctcct
    11341 tgatgacctt gggacaggca tcggcatcca cccaaatctg catgacctct ctcctgaaat
    11401 ttggccgcca tcataccctc ggtttgcgtc ggccaagcct gctcaacgac cgaccggacg
    11461 ccagagcagc ccgattgccg cgatcgcgca gatcgccgcc ccggcataga aggtgaacgc
    11521 ggcacccagc ctgtcccaca gcagcccggc cagcacgctg gccagcagca tcgccagccc
    11581 gctcatcaaa ttgaagaatc cgtaggccgt gccgcgcagg tcgccgggcg tcacgtcggc
    11641 caccatccgc gccagcaagc cctgcgtgat gcccatgtgg atgccccaca gcgcgacgcc
    11701 tgccagcacc gtgacccagt ggtcgtcggt cgccagcacc agatcggcgg cgatcagcac
    11761 cgccagcccc agcgccagca gtttggtgtg gctcatgcgg tcggagagtt ttccaaaggg
    11821 ataggccgag gccgcgtaga tcacgttcat cgccaccatc accagcggca ccagcgcgac
    11881 cgcaatgccg ccctgtgcgg cgcgcagcac cagaaaggcc tcgctgaaac gcgccagcgt
    11941 gaacaccgcc ccgatgccga ccacccacca ataggcggca ccgagacgcc tcaggttctc
    12001 gcggctgacc ggattgaccg gctgatgggc ctgcgttctc tccggctcat gcacgccgaa
    12061 cagcagcagc gccaccgcaa gcaatcccgg gatcaccgcc acccagaaca ccgcgcgaaa
    12121 atcgttggcc cacagcaaca tcaaccccac cgccagcaac ggcccgagga acgcgcccac
    12181 ggtatccaga gactggcgca gaccgaatgc cgcaccgcgt atcgccgcag gcgtgatgtc
    12241 ggcgatcagc gcatcgcgcg gcgcgccgcg cacgcccttg cccacccggt ccagaaaacg
    12301 cgcgcccagc accataccca tcgacgacgc cagcgcgaac atcggcttgg tgaacgcccc
    12361 caacgcatag ccgaacagcg ccaacccctt gcgccgtccc agatagtcgc tcagcgcacc
    12421 ggagaacacc ttcaccagca gcgccgtgga ttcggcgatg ccctcgatca cccccaccgt
    12481 caacgcgctc gccccgagca ccgtgaccat gaacaagggc agcaggctgt ggatcatttc
    12541 ggaggagaca tccatcagca ggctgacaaa tcccagcacc cacacgccac ggggtatctg
    12601 gagcagcgcg tctttagcga tcttgggcat gttttttacc ggaaaggcga tagaagaaat
    12661 aaggcgcgac cagcacccac aacaggatca gcggccacaa ccgcccttca gacagatcgt
    12721 agttcgccag cagcaccggc caggggtggc ctgcgacata atggaaaaac aggaactcga
    12781 atgccaccgt gagcagcatc cagaacaagc cgatgtacca cgcctcccgt gccgaaacag
    12841 gcggccagcg gcgcacatac tgatggatca ccacggcgaa cagcgcgatg ccgctcacgc
    12901 aggaaatctg atgcgcgagc aactcggaaa cctgcctgcc ataggtaaag tcgcgtagcc
    12961 cgccgttgat cacggcagcg gccagcagca cgaaccaggc aagcaggggc ttcatttcat
    13021 cttcgagggc agcttgatgg tatcgcccga taccctgaac acccccttgc cctgatggtg
    13081 cagcgtgcgc gggttcttcc gatcgggagc gatccacgcc ttgaccacct ccagcacgaa
    13141 gaagttgtac ttgttcacca tgcgggtgtc gaccactctg cattcgagat tcgcgtagca
    13201 ctcggctatc agcggcgcag ccaccataga ggcaggctgc tgcgtcagct tgaacttctt
    13261 gaatttgtcc accctctcgc ccgagcaatt gccgacgccg atgacctgct tcgccagctc
    13321 gaccgtcggg atgctcagca cgcattcctt agtcttcttc agcgccgaaa aactgaagtt
    13381 tcgcccgctg atcacgcaac ccaccagcgg cggctcgaac tccatcatcg tgtgccatga
    13441 ctgggtcatg atgttcgcgc ggcccttgtg ggcggtggtc accagcaaga ccgggccggg
    13501 ttcgagcagg cggtagactt tcgataacgg gaaagatttt ttggtcatca ccgcatatcc
    13561 tttcgcagaa gagatcaaat ctttatacca cccaccttgg tgaggttttg taggttgggt
    13621 ggagcttagc gatacccaac aaaacatttc aatcacatcc agaatgatgg gtattgttac
    13681 gctcaaccca tcctaccgcg ctgaagagcc atataacccg ctaaggataa agccgcgact
    13741 ggtggataac cctgacaatt ctgatcttag tgcgggtgac gcgatatatc aggttgtagg
    13801 gataacgggt gaagaccaat tcgcgcggag cgcccggctt gggtatccgc cccaatagag
    13861 gaaaacgggt aagaagttcg gcctgagcga tgaggtaatc tgttacttgc tcggcggcga
    13921 cagagttgtc ggatgaaatg tgggcatcta tcgccagcag gtcgtgcccg gaacggaccg
    13981 accattcaag ctgctttttt actctttgtt gcacgttgtt tggcgagcga ttcgcgaatc
    14041 tgtgcggtgg tgactacgtt gccgttgtcc aagtcggcct gcccttcggc tatggccttt
    14101 tccttccatt ccagatagtc gagcctgtcg gcaagcgccg ttttcaggat ggattcggga
    14161 ttgagatggg ctactgccgc gaccttttcc agccgcttgg cgagagttct gggcaatttc
    14221 aattcattca gcatggcgat ccctcgtgaa gtgcacctga ttctacagaa atatcgagta
    14281 catgacactg cggctgggca cccgacttac cggatcgaac aacatctttt gcgacactcg
    14341 cgtaaaatcc ctgccgcact atagtcccac ccatatccga aatccaccgc cctgaaagtc
    14401 cagatagctc ccgtgtccat cgaagtcgaa cgtttctttt ccgagcagag tccgcttgtc
    14461 accgaggtgg cgtcgttccg tccgcgcgcg cagcagcgcg agatggcgct ggcggtggcc
    14521 gaggcgatac gcgacaacgc gatcctggtg gcggaggccg gaacgggcac cggcaagacc
    14581 ttcgcctatc tggtgcccgc gctgctcaac ggcggcaagg tgatcatctc gaccggcacc
    14641 aagaacctgc aggaccagct gttccagaaa gacctgccca tggtgcgcga tgctttgcgc
    14701 gcgccggtgt cggtggcgct gctcaagggc cgctcgaact atgtgtgcca ctaccatctg
    14761 gctcgcgccg agtcggacgg ccagttcaag acgcgcgagg acgtgcgcca tctgggcaag
    14821 atcgtgaaat acgccaaggt cagcgattcc ggcgacaaga gcgggctggc cgacgtgccg
    14881 gagaacgcgc cgatctggat gcaggtgacc tcgacgcgcg acaactgcct cgggcaggaa
    14941 tgcccgaacc acaaggaatg cttcgtgctc aaggcgcgca ccgaggcgat gaaggcggac
    15001 atcgtggtgg taaaccatca tctgttcttc gccgacgtga tgctgcgcga cgagggcgtg
    15061 gcggagctgc tgcccgcctg caacacggtg atcttcgacg aggcgcacca gctgccggag
    15121 accgccagcc tgttcttcgg cgagaacatc tcgacttcgc atctgctgga actcgcgatc
    15181 gactcgcgca tcgaggccgc cgcctcggcc aaggacttcg cgccgctgcc caaggcctgc
    15241 gacgagctgg acaaggccgc gcgcgacctg cggctggtgt tcaagaaaga aggccgcatg
    15301 ccggccatcg cgacacagga tttccgcgag ttcgcgccct cactgaaagt cctcggcgag
    15361 aaactaaccc agctgtccgg cctgctggag aaacaggccg agcgcagcga ggggctggag
    15421 aactgctggc aacgcgcaca ggcgctatcg cagcaactgg cgaaatggca gaaggacgag
    15481 gagccggacc aggtgcgctg gctggagata ttccatcact cgctgcaact gaacaccacg
    15541 ccgctgtcga tcgccgagat cttcgagaag cagatcggcg gcaacgcgcg cgcctggata
    15601 ttcacctcgg cgacgctggc agtgaaacag gacttttcac actacttggg cgagatgggg
    15661 ctggcgaagg ccaggaccgc ctgctgggat agcccgttcg actacggtaa acagggcttg
    15721 ctgtatgtgc cgcagaacct gcccgagccg aacagcgaag gctacaccga ggcggtggta
    15781 caggccgcgc tgccgctgat cgaggccagc cgtggacgcg ctttcctgct gtttaccagc
    15841 ctgcgcgcga tgcagcgcgc ccacgaaatc ctgctggccg aattcgaccg gcgcggctgg
    15901 acctatccgc tgctgttgca gggcgaaggc tcgcgcaatg agctgttgag ccgcttccgc
    15961 gaccacggca acgcggtgct gctgggcagc cagtcgttct gggaaggggt ggacgtgcgc
    16021 ggcgaggcgc tgtcgctggt gatcattgac aagctgccct tcgcgccgcc ggacgacccg
    16081 gtgctggcgg cgcgtctgga tcagctgaag aaacaggggc gcaacgcctt catggaatac
    16141 cagttgccgc gcgcgatcat cacgctgaaa cagggtgcgg gacgattgat ccgcgacgag
    16201 aacgaccgcg gcgtgctgat gatctgcgac ccgcgcatca tcaccaagca atacggcaag
    16261 cgcgtgtggc agagcctgcc gccgatgaag cgcacccgcg acgaggcggt cgcggtggca
    16321 ttcttcacga cgccagcagt ccaggactag ctgccggtct tgtcgccccg atgcccggcc
    16381 ttaacgctct gctgtttcca ctgctcgtag cagtccagac cgcagaaatg cgcgacataa
    16441 tcggcgaccg cctccggcgt cttggcttcg gacagcggaa tctccttgcg gcagacagcg
    16501 caggcgactt tctcggtctc tacggggcta tttgagtcgt tcatgatgca cctcctgaga
    16561 agcttgaaca cggcaccaca ctattccgat ttgcgccgca cagcaagcat tgcggtctac
    16621 actgaccgcc ttgatgtccg gcataatttg agccgcatct gattctgtaa taaattgccg
    16681 cctgaattga acgaaagacc tgctgccccg gtctcacccg ggctcattgc aaccaccacc
    16741 gaggataacc ccatgaacaa caagttaaag aacctgatga tcgccgccgg catcatcgcc
    16801 tccttcagcc tgaatgcact ggctgcggat gccactcccg ccccgagcat gccggatcac
    16861 gcgcaacatc tggagcgcat gaagaccctg acacccgaac agcgagccaa ggaacaggag
    16921 gagatgcagc agaagatgca gagcctgaca ccggagcagc gtgccgaaca ccgcaaagcc
    16981 atgcgcgaac agtgggagaa gatgtcaccc gaagaccgca aggccatgca cgacgcgatg
    17041 cacgaggata tgcagggcat gtcgcccgat gaatgcaagg tgcgccatga ggccatggac
    17101 aagaacggca tgacgcccca gcaattcggt cacggcaacg ggcaacactg caatgtgaag
    17161 ccgcccaaag gcccttgccc ctatgacaag acaaagccga ccaaatagaa ccgccccggt
    17221 tgaatccaag ccccttactc gacattctgg atgcccatga atgaagcgat gctgaaattg
    17281 gtgcatggca ggaacgacct ctacccgcac gcgctggaga aaaagttcct gcgcatcttt
    17341 aaccggatcg tcgagctgtg ggatacaccg cagatggatg cctatttcac cgagctgatg
    17401 atggacacgc gcggcggctc tcgtcagggc tttcccaagg aggtcgtcgc cgagatctac
    17461 cagctgagcc ggatacatga acgttcgcaa cagcgggcgc ccgtgcagcc aagcgaccct
    17521 tgggcggaaa tcgagatcgg caagcgacgg gccattgaag agttaggcta cgaatgcacg
    17581 ccgcatgatt ttctgaaagc ggtggaaagc cagaacaaaa aggctctcgc cgcctatctc
    17641 agcgccggac tcccggtaga cacgcgcgac gagcgcggct ggacgccgct gatgatctcc
    17701 gccttcaatg gcaatgagga gatggccgag ctgttgatac gcagcggcgc cgatgtgcag
    17761 atcgaagacc atgccggtta cggaccgatg cactgggcgg cattcaatgg ctaccgcagg
    17821 gtggtcaggt tgttatccag ccagcgcggc aacgtgaatg cacgcagtca ccatggctgg
    17881 acgccgctgc tgcaagccgc cacacgcgga catctggatg tctgcatcgc gctgatatcc
    17941 agcggtgcca acgtcaatct gaccagcaac gacggttgga gcccgctgca caaggcctgt
    18001 gccaacgggc atattgaagt ggtgcaactg ctgctgtccc agggcgcgga tcgcaacgcc
    18061 cattatcagg atagcgtcac gccgctcgcc ctcgcgatca agaacaagcg cgaggacatc
    18121 gtggcgctgc tgaccagcgg cgtcatcgcc gacaagcgct agaccagctc cgcccacagg
    18181 tcgtgctcgt ccgagtcggt gacctttact tccaccagat cgccgacctg caactgctga
    18241 ccatcttcga tatagaccag accgtcgatc tccggcgcat cggctgaact gcgcgcgaca
    18301 gccccctcct cgtccacctc atccaccagc acggtgatgg tcttgccaat tttacgcttg
    18361 aggcgttccg tactgatctg ttcctgcaac agcatcagac gctccaaacg ttcctgctgc
    18421 acttcgggcg gcacatgatc cggcagttcg ttggcggccg caccttccac cggcgaatag
    18481 gcgaatgcgc cgacgcgatc cagttgcgcc tccttcagga attccagcag ctcctcgaac
    18541 tcggcctcgg tctcgccggg gaagccgacg atgaaagtgc tgcgcagcgt cagctccgga
    18601 catatctcgc gccagcgctt gatgcgttcc agcaccccgt cgctgttgcc cggacgcttc
    18661 atcagcttga ggatgcgcgg gctggcgtgc tgaaacggaa tgtcgagata gggcaatatc
    18721 ttgccctcgg ccatcagcgg aatcacctcg tctacatgcg gataggggta aacgtaatgc
    18781 aggcgcaccc acacacccaa ttcgcccagc gccacggcca gttcggtcat gcgcgtgcgc
    18841 agcggcctgc cgccccagaa accggtgcgg tatttcacgt cgacgccgta ggccgaggtg
    18901 tcctgcgaga tcaccagcag ctccttcacg cccgcgtcga ccagatgcct ggcctcctgc
    18961 atcacctcgc cgaccggacg gctgaccaga tcgccgcgca ggctggggat gatgcagaag
    19021 gtgcagctgt ggttgcagcc ctcggaaatc ttcacatagg caaaatgcct gggcgtcagg
    19081 cggatgccct gcgcgggcac cagatcggtg agcgggtcgt gcggcttggg caagtgctga
    19141 tgcaccgcct ccatgacctc atcggcggcg tgcgggccgg tcaccgccag caccttggga
    19201 tgcgcctgct gcaccacatc gcccttcgcg cccagacaac cggtgacgat gacacggccg
    19261 ttctcggcca gcgcctcgcc gatcgcgtcc agcgactctt ccaccgcact gtcgatgaaa
    19321 ccgcaggtat tcaccaccac cagatctgca tcctgataac tgcccgaaat cagataaccc
    19381 tccgcgcgca ggcgcgtgag aatctgttcg gaatccgagg tcgccttggg acaacccagg
    19441 gagacgaagc cgactttagg gggtgtgtgt ttttgcatcg ttaaatcttt ccgaatacgc
    19501 cgttaagcgc aagtaaatca ggcctgaacg aacaggctca tgatgtagat cgtcgccacg
    19561 cctgagccga tcaacagaac ctgctgcaag gtgtcgcgca gccgggtgcg cttgtgcagc
    19621 cccggaatca ggtcggctac ggcgatgtaa agcatgctcg ccgctgccag cgccagcagc
    19681 gtgggaatcc acgcctccat ggtctgcagg gtgaaatagc ccagtacgcc gcccgccacc
    19741 gaagcgaagc tggaaatcag attcagttga aaagccctga gcttgctgta acccgaatgc
    19801 agcaggatga cgaaatcgcc tacctcctgc ggaatctcgt gcgcgatgat cgccagcgcg
    19861 gtgaccatgc ccagatgcac gtcagtcagg aaggccgcag cgatgatgat gccatctaca
    19921 aaattgtgaa aggtatcgcc caccgtgatc atcaagccgg tacgaccgtg atcgtggctg
    19981 tgcgcaaccg caggcgactc atgcgcctcg caatgctcgt ggtgacagtg ccgccacagc
    20041 accagtttct ccagaatgaa gaacagcatg atgccgaaca gcaccgttgc gctcagcgcg
    20101 gccacattgg tggagagtgt gatcgcctcg ggcaggatgt cgaggaacac cgcacccaac
    20161 aatgcgccaa tggcatagct caccatcgca ccgagatagc gctgcatgtg ggagttaaac
    20221 gcaaacagcg ccgcacccag cacgctcaac acgccgccgg cgatgctggc gatgacgatc
    20281 caaagcagaa tggtcatgga attcattcga tcaatca
//
LOCUS       DASUIV010000085         8752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_112826, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000085 DASUIV010000000
VERSION     DASUIV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8752
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112826"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..771)
                     /locus_tag="VFW59_03145"
     CDS             complement(268..771)
                     /locus_tag="VFW59_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5363240.1"
                     /translation="MTTLSRLHIDIEVRVQTILGERSDWLCGKGCDSCCRQLANVPQL
                     TQAEWDLLREGLADLAPERLQEIRRNMAALSSQRSRPYTCPMLDISTGSCPVYAQRPV
                     ACRTYGFYVQRDQGLYCSDIESRVADGSLADVVWGNHDVIDQRLAGLGEVRALTDWFE
                     RWNVKNE"
     gene            complement(1170..1451)
                     /locus_tag="VFW59_03150"
     CDS             complement(1170..1451)
                     /locus_tag="VFW59_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX5363241.1"
                     /translation="MTRADLIEQLAQKYPQLMLKDVDLAVRLILDEMSLTLSKGDRIE
                     VRGFGSFGINYRPPRNGRNPKTGMAVPVPAKYAPHFKPGKELRERVDRG"
     gene            1792..2349
                     /locus_tag="VFW59_03155"
     CDS             1792..2349
                     /locus_tag="VFW59_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363242.1"
                     /translation="MIFDSYEQKGLLFNMNFKEANGNYAAYRGDLVLELGEVGDALGH
                     RKPPVATIKNTIVLSENDKIKLYVGSLDELALLPKVLDYYQADFAADVLLILFVVNIN
                     KPMVIETGGFSIAAIGMQEGLIWNELIDLAALDKGDFKGQSASEKIITVYKALSDFKP
                     KGDKVSFDEAMSRTVELKRAGRGPV"
     gene            2417..2959
                     /locus_tag="VFW59_03160"
     CDS             2417..2959
                     /locus_tag="VFW59_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEX5363243.1"
                     /translation="MSRIGLFFSSSTGNTRRIAKAIKKRFDDDTMADALNVNKATPEL
                     FASYSHLILGTSTLGAGALPGLSTDCLGGGWEEFLPKIEKLDFSGKTIALFGLGNQDK
                     YPDEFVDGIGILYEFFTARGAQVVGFWPADNYDFISSRALLDGEFVGLALDQENQKLL
                     TEARVEQWLKLIAPAFGLAP"
     gene            complement(2997..3323)
                     /locus_tag="VFW59_03165"
     CDS             complement(2997..3323)
                     /locus_tag="VFW59_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363244.1"
                     /translation="MPEQTAMPFDVGSRQHRVFGKQVRLQFAEDFVLRLTAAEASSLS
                     FALVAVRDGISPEREIYMSPIGSDHEFVGTVCDSGMKIAIGKDVLALDWVGVGKLAGA
                     LAAAIE"
     gene            complement(3316..3780)
                     /locus_tag="VFW59_03170"
     CDS             complement(3316..3780)
                     /locus_tag="VFW59_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5363245.1"
                     /translation="MNDNELRCWLLSEGFGISYPQQIDIDQTVGNDTTPLMHATRTGN
                     VEVVHALLARGADPALMNADGNGALWFACFANSEACATALIAAGAPLDSQNVNGATAL
                     IYCASSGKTPLVRLLLDAGANPGLSTLDDFTALELASNIRCMRMLREVAVHA"
     gene            complement(3796..5322)
                     /locus_tag="VFW59_03175"
     CDS             complement(3796..5322)
                     /locus_tag="VFW59_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5363246.1"
                     /translation="MGETDVARVLSYHARSKHALDRYAAGPGTLDWDAQPEAFRDWAG
                     TQQTALPREVRVADISWGALAVSRPPAPLDAAHLGSLLRLCVGLTAWKEYGASRWSLR
                     AHPSSGNLHPTETWLIAAGAGDIADGLYHYQNRNHALERRAWGGEADAQGVWLGFSSI
                     HWREAWKYGERAFRYCQLDMGHVLAAVSYAAALHGWKPCLLNLEPSALAHGLGLDREA
                     DFDGVEGEEAEVVVALTSDDPLPMSWQHWEGLPSLLDPRPLYRWPVIDEVATATRGRG
                     PDALVAAAVAPRAADEIGAARVILKRRSAQAFDGESVMPQALFGRMLASLMPGGSPVW
                     ELWPHAARVHPVLLVHRVEGMAPGLYALPRSEAARGALQAAFREAFSWQPADSGLPLF
                     QLVAARAGKTARTLSCHQDIASQCAVTFMMVAEFAAPVAADPAAYRHLHWEAGMLGHV
                     ITLEAEAAGWRGTGIGCFFDDADHEVLGLKDGQFQVIYHYAVGMALDDPRISMLPAYE
                     "
     gene            complement(5323..5592)
                     /locus_tag="VFW59_03180"
     CDS             complement(5323..5592)
                     /locus_tag="VFW59_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HEX5363247.1"
                     /translation="MSRFQMGDLVFAAQDLYNEVVEESGESGIPGVAPDALLAVTGTR
                     GVVVNIGHAQEMPNEEIYLVRFETDAEGTLAEPIGCLSEELSEAA"
     gene            complement(5597..5959)
                     /locus_tag="VFW59_03185"
     CDS             complement(5597..5959)
                     /locus_tag="VFW59_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363248.1"
                     /translation="MRSGQLPAELAAMAGRAVLALQQEAKRYSFPAPQIGLEALQFTR
                     VIDPGNQLPGYEGVWRNARNDRCGTLTINSDGSFYAEYDLFCPHPSDKRWFVEMVTAW
                     GGGDVLRCEAQLIPSLEE"
     gene            complement(6017..6340)
                     /locus_tag="VFW59_03190"
     CDS             complement(6017..6340)
                     /locus_tag="VFW59_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX5363249.1"
                     /translation="MPKPERHVFVCSQNRPAGHPRGSCTQNGCAEVVDEFMKQWMQRQ
                     CFAQVMVTPSGCIGPCSEGPNVLIYPEGVMYSHVTKEDVGEIFDSHLLGDKVVERLKT
                     PADKW"
     gene            complement(6343..6633)
                     /gene="fdxB"
                     /locus_tag="VFW59_03195"
     CDS             complement(6343..6633)
                     /gene="fdxB"
                     /locus_tag="VFW59_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HEX5363250.1"
                     /translation="MANITGITRGGTPYEPTFVTELNAANCIGCGRCYKVCPRTVFEL
                     VERSEDDENYDEDEDNSMVMTLANALDCIGCGSCGRVCPKQCHTHQAMPAGA"
     gene            complement(6636..6848)
                     /locus_tag="VFW59_03200"
     CDS             complement(6636..6848)
                     /locus_tag="VFW59_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HEX5363251.1"
                     /translation="MTEEELKTLDKEVKRLKRISSEWASQLHDLVEERLPAGYQEIPG
                     IAQSTYDACRAWEEANIKLMAAQKET"
     gene            complement(6936..7394)
                     /locus_tag="VFW59_03205"
     CDS             complement(6936..7394)
                     /locus_tag="VFW59_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HEX5363252.1"
                     /translation="MTQAAEQDLLSTDFIKEMVKQMRAIDSYGTYDGWPADRILAPYV
                     VTKEQRREIPVIGDPDEIMLARIKAFYNAISSLIEKECGLMAVPSVNITHEGFGRALI
                     TVGKLVVMDKTLRDVHRYGYESLSKMKDEGDKLLSVALALIGKYPEVAGL"
     gene            complement(7391..7828)
                     /locus_tag="VFW59_03210"
     CDS             complement(7391..7828)
                     /locus_tag="VFW59_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HEX5363253.1"
                     /translation="MISTSARVISVADGTARLDPGMQSGCGGCKSRSSCGVSGLGKYF
                     SGNRKAIEVKCDASIRAGDELQLSMSEGDLLKAGLLAYLLPSVLALVVAGIASSLGLG
                     DAGAVFGAGIGFAAGFLLVRLSGWVPRMNVQHKNENFSKGETP"
     gene            complement(7825..8238)
                     /locus_tag="VFW59_03215"
     CDS             complement(7825..8238)
                     /locus_tag="VFW59_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX5363254.1"
                     /translation="MTVKIGFATSDRRAVNQHFGAAGAFAIYEVGEHETRLVEVAEFI
                     ETAMDGHEGKLAAKVELLGDCAAVYCNAAGASAIQKLLAGGIQPMRVDEGTPIEELLL
                     GLQASLLNEPPLWLAKHLKKQSGGDKFADDEEWQE"
     gene            complement(8249..>8752)
                     /locus_tag="VFW59_03220"
     CDS             complement(8249..>8752)
                     /locus_tag="VFW59_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX5363255.1"
                     /translation="AQLQDAMVDSHFMLGFARVAIAADADLLYGYSRLVTEMGGEVVA
                     AVAPARANILKDVIAEQVAIGDLEDLEIAAKKNGAQLVISNSHAVETARRLAVPLLRA
                     GFPQYDLLGGYQRLWVGYRGTRQTLFDLANLLVEHGQHEQEAYVSIYQRQTKEANHAT
                     SAASQTH"
BASE COUNT         1754 a         2818 c         2420 g         1760 t
ORIGIN      
        1 aaaagaaaaa gtgttatttc cgttgagaaa taacactttt tgaatatatg gtgcgcccga
       61 agagatttga actcctgccc ccttggtttg caagcaactt ttcaaacgca gctatattga
      121 ttttatttat cccgtgagag actggccgtt gcatccatgc cctatagcgc actacgcagc
      181 acgactgtgt cccgcaaaga aatgcctatg agagcgagca gggagttaat gcacgacgtc
      241 ctcacttacg cgaaagctat ttcccaatta ctcatttttc acgttccaac gctcgaacca
      301 gtcggtcaag gcacggactt cgccaaggcc ggcgagccgc tgatcgataa catcgtgatt
      361 accccacacc acgtctgcca aggagccatc ggccacgcgg gattcgatat cggaacagta
      421 aagtccctgg tcgcgctgaa catagaagcc gtaggtgcgg cacgccaccg gacgctgcgc
      481 atagacagga caggagccgg tagaaatatc cagcatagga caagtgtagg gtcgcgaccg
      541 ctgactgctc aacgcggcca tgttcctgcg gatttcctga agtcgttccg gcgcgaggtc
      601 tgccaggccc tcacgcaaca agtcccattc agcttgcgtt agttgaggca cgttggcgag
      661 ctgccgacag caactatcgc agcccttccc acacaaccag tcggaacgtt ctcccagaat
      721 ggtttgtacg cgcacctcaa tatcgatatg tagccgggat agcgtggtca tagttgttca
      781 cttaaattgt gccggccagc cttacctcgt tagaggcgtc taatcgagtc tgcgggggcg
      841 agtcccgcaa tgacgttgct gaaaagaaaa atcgcctagc atttcacgcc aagcatttga
      901 ttttagtgca aaatatggtg caccagaaga gattcgaact cctgccccct tggttcgtag
      961 ttcgggactt tgatagcaac caattgttta tatttgattt attaacaaac cccacatccc
     1021 cttcaagcac tgtacttcag tgttctctac aaccctgttc cttcaaagtt ccttcagaat
     1081 taaacaaaaa ctggttttta tctaccatat gatgcgatta ataagcgttc gcgtgggatg
     1141 tacaaagcca ttttgagttg gaatggcaat cagcccctat ccaccctctc ccgtaactct
     1201 ttaccaggct tgaagtgcgg cgcatatttg gcaggaaccg gtacggccat acctgtcttc
     1261 gggttgcgcc cattccgtgg cggccgatag ttgatgccaa aactgccaaa gccacgtacc
     1321 tcgattcgat cccccttact caaggttagg ctcatttcat cgagaatcag gcgaacggcc
     1381 aggtcgacgt ctttgagcat gagttgaggg tacttctgag caagctgttc aatgaggtct
     1441 gcgcgggtca tgaagaaaag ccagttgatc tggcttgtat tgattatgca aacaaggcgt
     1501 taaggcttcg aattataccg acatgagccc catcaggaat accgaatgaa ctttctattc
     1561 aataggcact ctatgcttac gttggcgaaa gccatacaca cgtctcctcc ctgaacgtgt
     1621 atctcgactc tctgttgaga ctgtttgccc ctgagccata ggcttcaggg gcattttttc
     1681 tgatccaatc tgatcccggc ctgttcccga catcgagaat catccttccg ttggctggta
     1741 tggatattgc tgaataggag gctccatcaa tcaaaaacga cgaggagcat catgatattc
     1801 gattcttacg agcaaaaagg cctgctgttc aacatgaatt tcaaagaggc caacggcaac
     1861 tacgcggcct atcgcggcga tctggtgctg gagctcggcg aagtcggcga cgcgctcggt
     1921 caccgcaagc cgccggtcgc gaccatcaag aacaccatcg tcctgtcgga aaacgacaag
     1981 atcaaactgt acgtcggcag cctggacgag ctcgcgttgc tgcccaaggt gctggactac
     2041 taccaggccg atttcgccgc agacgtgctg ctaatcctgt tcgtcgtgaa catcaacaag
     2101 ccgatggtga tcgaaaccgg cggcttctcg atcgccgcga tcggcatgca ggaaggccta
     2161 atctggaacg agctgatcga tctggctgcg ctggacaagg gcgacttcaa gggccagtcc
     2221 gcctcggaaa aaatcatcac ggtgtacaag gcactgagcg acttcaagcc caagggcgac
     2281 aaggtcagct tcgacgaagc gatgtcacgc acggtcgaac tgaagcgcgc aggacgcggc
     2341 ccggtctaag tcagcagccc tatccgctgc cgattgtcgg aaacattaca atcggcagcg
     2401 tcttcgaggt gtcaccatgt cacgcatcgg attatttttc tccagcagca ccggaaacac
     2461 ccgccgtatc gccaaggcga tcaaaaagcg tttcgacgac gacacgatgg ccgacgcgtt
     2521 gaacgtcaac aaggcgacgc cggagctgtt cgcaagctac agccacctga tactgggcac
     2581 gtctacgcta ggcgcaggcg cgctgcccgg actctcgacc gactgcctgg gtggcggctg
     2641 ggaagagttc ctgccgaaaa tagagaagct tgatttcagc ggcaagacca tcgcgctgtt
     2701 cgggctcggc aaccaggaca aatacccgga cgagttcgtc gacggaatcg gcatactcta
     2761 cgaattcttc accgcaaggg gcgcgcaagt ggtcggcttc tggcccgccg acaactacga
     2821 cttcatctca tccagggccc tgctcgacgg cgagttcgtc ggcctggccc ttgatcagga
     2881 gaaccagaag ctgctcacag aggcgcgggt ggagcagtgg ttgaagctga tcgcacccgc
     2941 gttcggactg gcgccttaac ccgctatcct caaggaaggc atcaaccgcc cagacgctat
     3001 tcgatcgcgg cggccagcgc accggccagt ttcccgacac ccacccagtc cagcgccagc
     3061 acgtccttgc cgatggcgat cttcatgccg ctgtcgcaca ccgtcccgac aaactcgtga
     3121 tcgctgccta tcgggctcat gtagatctcg cgctccgggc tgatgccatc gcgcaccgcg
     3181 accagcgcga acgaaaggct ggatgcctct gccgcagtca gcctcagcac gaagtcttcg
     3241 gcgaactgca ggcgcacctg tttgccgaag acccggtgct gcctgctgcc cacatcaaag
     3301 ggcatggccg tttgctcagg catgcacggc gacctccctg agcatcctca tgcagcggat
     3361 attcgaggcc aactccagtg cggtgaaatc gtccagcgta ctcaagcctg gattggcgcc
     3421 cgcatccagc agcaggcgca ccagcggcgt cttgccggat gaggcgcagt agatcagcgc
     3481 ggtcgcaccg ttcacgttct ggctgtcgag cggcgctccg gccgcgatca gtgcggtcgc
     3541 acaggcctcg ctgttcgcga aacaggcgaa ccacagcgcg ccattgccat ccgcattcat
     3601 cagcgccgga tcggcgccgc gcgccagcag cgcatgcacc acctcgacat ttccggtccg
     3661 ggtcgcatgc atcaggggtg tggtgtcatt gccgacggtt tggtcgatat cgatctgctg
     3721 cggatatgag ataccgaatc cctcgctcaa cagccagcac ctgagttcgt tgtcattcat
     3781 ctcaacccct cttcatcatt cgtaggcggg caacatcgag atgcgcggat cgtccagcgc
     3841 catcccgact gcgtagtggt agatcacctg gaactgccca tccttcaacc ccagcacctc
     3901 atgatccgca tcgtcgaaga aacagccgat gccggtgccg cgccatccgg cggcctcggc
     3961 ctccagcgtg atcacatggc ccagcatgcc cgcctcccag tgcaggtggc gataggcggc
     4021 cggatcggcg gcgacgggtg cggcgaactc ggccaccatc atgaacgtga ccgcgcattg
     4081 cgaagcgata tcctggtggc aggacagcgt gcgggccgtc ttgcccgcgc gcgcggcgac
     4141 cagttgaaac aggggtaaac cgctatccgc aggctgccag ctgaacgcct cgcggaatgc
     4201 ggcctgcagc gcgccccggg cggcctccga gcgcggcagc gcatacaggc cgggggccat
     4261 gccttcgacc ctatgcacca gcagcaccgg atgcacccgc gccgcatgcg gccacagctc
     4321 ccagaccggc gagccgccgg gcatcaggct ggcgagcatc cgcccgaaca gggcttgcgg
     4381 catcaccgac tcgccgtcga aggcctgcgc gctgcgccgc ttcaggatga cccgggccgc
     4441 gccgatctcg tccgccgccc gtggcgcaac cgccgccgcg acgagcgcat ccggccccct
     4501 gccgcgcgtc gcggtggcca cctcgtcgat gacgggccat cggtacagcg ggcgcggatc
     4561 gagcaggctg ggcagacctt cccagtgctg ccagctcatc ggcaacggat catccgacgt
     4621 cagcgcgacg accacctccg cctcctcgcc ttccacaccg tcgaaatccg cctcgcgatc
     4681 cagccccagc ccatgtgcca gcgcggaggg ttccagattc agcaggcacg gcttccagcc
     4741 gtgcagggcc gccgcatagc tgactgcggc caacacatga cccatgtcga gctgacagta
     4801 acggaaagcg cgctccccgt atttccatgc ctcgcgccag tgtatggaac tgaaacccag
     4861 ccacacgccc tgcgcatcgg cctcgccgcc ccaggcgcgc cgctccagcg cgtgattccg
     4921 attctggtag tgatacaggc cgtcggcgat atcccctgcc ccggctgcga tcagccaggt
     4981 ctcggtggga tgcagattgc cggacgaagg atgggcgcgc agcgaccagc gcgaagcgcc
     5041 gtattctttc catgcggtca ggccgacgca caggcgcaac agggaaccga gatgagcggc
     5101 atccagcggt gcgggaggac gggatacagc cagcgctccc cacgagatgt ccgctacccg
     5161 aacttcgcgc ggcaaggcgg tctgctgcgt ccccgcccag tcacggaaag cttctggctg
     5221 ggcatcccaa tccagcgtgc cgggcccggc cgcatagcgg tccagcgcat gtttgctgcg
     5281 cgcgtgataa ctcagcacgc gagcgacatc ggtctcgccc atctaggcgg cttcgctcaa
     5341 ctcttcgctc aggcaaccga tcggctcggc cagcgtaccc tcggcgtccg tctcgaaacg
     5401 caccaggtag atctcctcgt taggcatctc ctgcgcatgg ccgatattca cgacgacgcc
     5461 gcgcgtacct gtcacggcca gcaaggcatc gggagcaacg ccggggatgc cgctctcgcc
     5521 gctttcctcg acgacctcgt tgtacaggtc ctgcgcggcg aataccaggt cacccatttg
     5581 aaatctgctc atcgctctac tcctccagac tcggaatcaa ttgtgcctcg caacgcagta
     5641 cgtccccgcc gccccacgcg gtgaccatct cgacgaacca gcgcttgtcg ctcggatgcg
     5701 ggcagaacag atcgtactcg gcatagaagc tgccgtcgct gttgatcgtc agcgtcccgc
     5761 agcggtcgtt gcgcgcattg cgccacactc cttcgtaacc gggaagctgg ttgcccggat
     5821 cgatgacccg ggtgaactgc aatgcctcca gcccgatctg cggcgcggga aagctgtaac
     5881 gcttcgcctc ctgttgcagc gcgagcacgg cccgcccggc catcgcggcc aattcggcag
     5941 gcagctggcc agacctcatc ccggctccgt tccgacagtc agacaacgcg acatcaccga
     6001 tgatacgaag ctggaattac cacttgtccg ccggcgtctt caggcgctcg accaccttgt
     6061 cgcccagcag gtgagaatcg aatatctcgc cgacgtcttc cttcgtgaca tggctgtaca
     6121 tcacgccctc gggatagatc agcacattcg gcccctcact gcacgggccg atgcaaccgc
     6181 tcggcgtcac catcacctgg gcgaagcact ggcgctgcat ccactgcttc atgaactcgt
     6241 ccaccacttc ggcgcagccg ttctgggtgc aggaaccgcg cggatggcca gcaggccggt
     6301 tctgggaaca aacgaatacg tgtctttctg gttttggcat gattacgctc ctgctggcat
     6361 ggcttgatgg gtgtggcact gcttcggaca gacgcgcccg cacgagccgc agccgatgca
     6421 gtcgagcgca ttggcgagcg tcatcaccat gctgttgtct tcgtcctcgt cgtagttctc
     6481 gtcgtcctcg ctgcgttcga ccagctcgaa cacggtgcgc gggcagacct tgtagcagcg
     6541 gccgcagccg atgcagttgg ccgcattcag ttcggtgacg aaggtcggct cgtaaggcgt
     6601 gccgcctctt gtgattcctg tgatgttagc catgactatg tttccttctg tgcggccatt
     6661 aatttgatat tcgcctcttc ccaagcccga caggcgtcgt aggtggattg cgcgatgccg
     6721 ggaatctcct ggtatccggc cggcaagcgt tcctcgacca gatcgtgcag ttgcgatgcc
     6781 cattcgctgg aaatgcgctt gagccgcttc acttccttgt cgagtgtttt cagttcttct
     6841 tcggtcattt ttaactcctg gtaagtagta agtggtgagt ggtgtgtggc gctgcttcaa
     6901 ctacttacca cttcctactt accacttacc aatctttaca gcccggccac ttccggatac
     6961 ttgccgatca gagccagtgc gacagacaac agcttgtcgc cctcgtcctt catcttggac
     7021 aggctctcgt aaccgtagcg gtgcacgtcg cgcagcgtct tgtccatgac caccagcttg
     7081 ccgacggtga tcagcgcacg gccgaatccc tcgtgggtaa tgttgacgct gggcaccgcc
     7141 atcaggccgc actctttttc gatgagtgaa gaaatcgcgt tataaaaagc cttgatgcgc
     7201 gccagcatga tctcgtccgg atcgccgatc acggggatct cgcggcgctg ctccttggtc
     7261 accacatatg gtgcaagaat acggtcggca ggccagccgt cgtaggtgcc gtaactgtcg
     7321 atggcgcgca tttgcttgac catctctttg atgaaatcgg tggacaacaa atcctgttct
     7381 gctgcttgag tcatggtgtt tctcctttag aaaagttttc gttcttgtgt tgcacattca
     7441 tgcgcggcac ccaaccggac agccgcacca gcaaaaatcc cgccgcaaat ccgatacccg
     7501 cgccaaatac cgcaccggca tcgcccaagc ccagcgagga cgcaatgccc gccacgacca
     7561 gcgccaacac gctgggcaac agataggcca gcagcccggc cttgagcaga tcgccttcgc
     7621 tcatgctcaa ctgcaactcg tcgcccgcgc ggatgcttgc gtcgcacttc acttcgatgg
     7681 ccttgcggtt gccggaaaaa tatttcccca agcccgacac cccgcaactg ctgcgcgact
     7741 tgcagccgcc gcagccggac tgcatacccg gatcgagccg tgctgtgcca tcggcaacgg
     7801 atatcactct tgctgaagtc gaaatcattc ttgccactcc tcgtcatccg cgaacttgtc
     7861 gccgcccgac tgcttcttca aatgtttcgc cagccatagc ggcggctcgt tgagcagact
     7921 ggcctgcagg cccagcagca gctcttcgat cggcgtgccc tcgtccacac gcatcggctg
     7981 tatgccgccg gccagcagtt tttgtatcgc cgaggcaccc gccgcattgc agtacaccgc
     8041 cgcacaatcg cccagcagct cgaccttcgc ggccagcttc ccctcgtgtc cgtccatcgc
     8101 tgtctcgatg aattcggcca cttcgaccag ccgagtctcg tgctcgccga cctcgtagat
     8161 ggcgaacgcc ccggccgcgc cgaagtgctg attgaccgcg cggcggtcgc tggtcgcaaa
     8221 acctatcttc acggtcatgt tcgcatcctc aatgggtttg actagccgca gatgtcgcat
     8281 ggttagcttc tttggtttgg cgttgataga ttgaaacgta cgcttcctgc tcatgctgcc
     8341 cgtgctccac cagcaggttc gccagatcga acagcgtctg tctcgtgccg cgatagccga
     8401 cccacaggcg ctgatagccg ccgagcagat cgtattgcgg gaagcctgcg cgcagcagcg
     8461 gcacagcgag acggcgcgcg gtctccaccg catgcgagtt gctgatgacc agttgcgcac
     8521 cgttcttctt cgcggcgatc tccaggtctt ccaggtcgcc gatcgcgacc tgctccgcaa
     8581 tcacgtcctt caggatgttg gcgcgcgccg gggcgactgc ggcaaccacc tcgccgccca
     8641 tctcggtcac cagccgcgag tagccgtaga gcaggtcggc atcggcggcg atcgccacgc
     8701 gcgcgaagcc cagcatgaaa tggctgtcca ccatcgcgtc ctgaagctgt gc
//
LOCUS       DASUIV010000086         7388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1229560, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000086 DASUIV010000000
VERSION     DASUIV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229560"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..714)
                     /gene="ruvA"
                     /locus_tag="VFW59_03225"
     CDS             complement(130..714)
                     /gene="ruvA"
                     /locus_tag="VFW59_03225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5363256.1"
                     /translation="MIGRLTGILIEKNPPQILLDVQGVAYEVDVPMSTFYNLPVLNER
                     VMLHTQLIVREDAHLLYGFASLDERAAFRQLLKITGVGAKMALSVLSGLSLSDLAAAV
                     ATKDAARLTRIPGVGKKTAERLLLELQGKFVTAALAGNAAANITASANDDIVNALQAL
                     GYNEKESDWAAKQLPKDVDVSGGIRQALKLLSKS"
     gene            complement(711..1661)
                     /locus_tag="VFW59_03230"
     CDS             complement(711..1661)
                     /locus_tag="VFW59_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX5363257.1"
                     /translation="MTQPLEPTLEVLRNPVARYVAATRPAFLSASLMACCIGLAIARH
                     DGLVINAELAVVTLLFALMAQAGTNVLNDYYDALNGTDAQNVERIFPFTGGSRFIQNG
                     VLTQGQTRNFGFALMAAVAAAGLWLMAHSAAQLFYIGLAGLFIGWAYSATPFKLNSRG
                     WGELCVAAGFLLIAVGTDFVQRREFATAPFIAGLSYALLVTNLLYINQFPDRRADTAA
                     GKLHWVARLEVGQARWGYALIVALAYLWLLAAVALGALPVWALLTLLALPLSIKAARQ
                     LLQHAAQPQQLADAIKLTIAAMLAHGALLSLALILSKGNT"
     gene            complement(1658..2215)
                     /gene="ruvC"
                     /locus_tag="VFW59_03235"
     CDS             complement(1658..2215)
                     /gene="ruvC"
                     /locus_tag="VFW59_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5363258.1"
                     /translation="MPSSILNPQSSIRILGIDPGLRITGFGVIDKEGQQLHYVASGCI
                     KTPDGELPGRLRVILDSLREVIELHRPHLASVEKVFVNVNPQSTLLLGQARGAAICAA
                     VLADLPVAEYTALQVKQAVVGNGHADKTQVQAMVVRLLKLSGTPSPDAADALACAICH
                     AHGGMGGMATLAATKGFRVRGGRMV"
     gene            complement(2261..2986)
                     /locus_tag="VFW59_03240"
     CDS             complement(2261..2986)
                     /locus_tag="VFW59_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5363259.1"
                     /translation="MAGHSKWANIQHRKGKQDAKRGKVFTRLIKEITVAARMGGGDAG
                     ANPRLRLAIEKARENNMPKDTVENAIKRGSGQLEGVNYEELRYEGYGINGAAVMVDCM
                     TDNKTRTVGDVRHAFSKHGGNLGTDGSVAFLFNHCGQMIFAPGTDENGLMEVALDAGA
                     EDITTNDDGSIEVITAPYEFVNVKQALEAAGYKPEFGEVIMKPANEVVFTGDDAVKMQ
                     KLLDALENLDDVQEVYTTAVIED"
     gene            3228..4697
                     /locus_tag="VFW59_03245"
     CDS             3228..4697
                     /locus_tag="VFW59_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX5363260.1"
                     /translation="MNEPLLIAKNDQHELCLLPQMANRHGLITGATGTGKTVTLQTLA
                     ESFSRIGVPVFLSDIKGDLSGLSKAGGGNAKVAARVQQLGLSDFTHRAYPVTFWDVFG
                     KMGHPLRATVSDMGPLLLGRMLNLNEVQQGVLALVFKIADDNGLLLLDLKDLRAMVQH
                     VGENAGDFTTEYGNISTASVGAIQRGLLQIETQGGDKLFGEPMLNIEDLMQTDANGLG
                     MINILAADQLMTSPKVYSTMLLWLLAELFENLPEAGDLAKPKMVFFFDEAHLLFTDAP
                     AVLLDKNEQVVRLIRSKGVGVYFVTQNPADVPDKVLGQLGNRVQHALRAFSPRDQKAV
                     KAAAETMRDNPELDEAAAITELGVGEALVSCLDEHGRPGIVERAFVVPPQAQIGPIDT
                     AERSAVIAGSLLAGHYEKAVDRESAYEILKGRTAEKQAAASTAPRGESGGVGGMLGGG
                     SRREGAVEALAKSAARAIGSEVGRQIIRGVLGSLLGGRR"
     gene            4733..5341
                     /locus_tag="VFW59_03250"
     CDS             4733..5341
                     /locus_tag="VFW59_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5363261.1"
                     /translation="MKTILVANPKGGSGKSTLSVNIAGYLANRGQHVAMLDLDRQQSS
                     ALWLSMRSDELPPIHSLDPKKKDDRAYDWLVIDSPAGLHGKNLAHALRLANKIIVPVA
                     PSLFDLQASQDFLRLLGEEKAGRKGKCHIGVVGMRMDGRTRAAQALEHFLSSLDLPVL
                     AYLRETQVYVNAAFEGKSLFDLPPYLAERELEQWTYLLDWLE"
     gene            complement(5342..6286)
                     /locus_tag="VFW59_03255"
     CDS             complement(5342..6286)
                     /locus_tag="VFW59_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5363262.1"
                     /translation="MASKMVPFSEEVNSLFANSDANEVWDKAASIILLINPDCDLAPM
                     RAVFDDMVALFHGEYPAYAPVKTLYHDLAHTLHVFLCAVRLMHGVHVSGTPLRDEDVV
                     LVMTAALMHDIGYAQASNEEAGTGAQYTLCHVSRGIEFMHNYLAAKGWPADLATRIEP
                     LIRATDPSFPFAQIAFADEHQRLLGQLVATADLVGQMADRTYLEKLLFLYLEFKEAHF
                     GNYQNVHDMLRQTKRFYDLTREKLDVVYGGVYARLSAHFRVTMGVGNNYYLESIEKNI
                     DYLARVTQLEEAEHFTMLKRGGIADKAKTLPDFQAPQG"
     gene            complement(6320..>7388)
                     /gene="ychF"
                     /locus_tag="VFW59_03260"
     CDS             complement(6320..>7388)
                     /gene="ychF"
                     /locus_tag="VFW59_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5363263.1"
                     /translation="GLPNVGKSTLFNALTKSGIAAENYPFCTIEPNVGIVEVPDPRLE
                     ELAKIVKPQKMQHAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVNVVRCFDDE
                     NVVHVAGKVDPLSDIEVILTELALADMAVVERTIQRDGKKAKSGDKDAQKLVALLEKL
                     LPHLNEGKPARTFPLTDDDKLLLKPLCLLTIKPAMYVGNVLEDGFENNPHLDRLREHA
                     AKEGAPVVALCAKIEAELADLDDADKQEFLADLGLDEPGLNKLIRTGYELLGLQTYFT
                     AGVKEVRAWTIHKGDTAPQAAGVIHTDFEKGFIRAQTISYADFIACGGEAGAKEKGKM
                     RVEGKEYVVQDGDVMNFLFNV"
BASE COUNT         1410 a         2381 c         2194 g         1403 t
ORIGIN      
        1 agctgcttcg gccgcagcgc gcgctccagc gcctcctcct gacgcgaggc gggagcggcc
       61 gaaatgatgc gagatgcggc ggaaaggtcg tcgttgtgga tcatgccttg gataataact
      121 taagtgactt cacgatttgg acagaagctt caatgcttgg cggataccgc ccgagacatc
      181 gacgtccttg ggcagttgtt tcgccgccca gtcggattcc ttctcgttgt agcccagcgc
      241 ctgcaaggca ttgacgatgt catcgttggc agaggcggtg atgttcgctg cggcattgcc
      301 cgcgagtgcg gcggtgacga acttgccctg cagttccagc agcaggcgct cggcggtctt
      361 cttgccgacg ccgggtattc tggtgagccg tgccgcatcc ttggtcgcaa ccgctgcggc
      421 gaggtcggac aggctcaggc cggacagcac cgacagcgcc atcttcgcgc cgacgccggt
      481 gatcttgagc agctgacgga aggcggcgcg ctcgtccaga ctggcgaagc cgtacagcag
      541 atgcgcgtct tcacgcacga tgagctgggt atgcagcatc accctctcgt tcagcaccgg
      601 caggttgtag aaggtgctca tcggcacgtc cacctcgtag gccacgccct gcacgtccag
      661 caaaatctgc ggcgggtttt tctcaatcaa aatcccagtc aatcttccga tcatgtgttt
      721 cccttactta aaatcaatgc gagcgacagc agcgccccat gcgccagcat cgcggcgatg
      781 gtcagcttga tcgcgtcggc cagttgctgc ggttgtgcgg cgtgctgcaa caactggcgc
      841 gctgccttga tgctcagcgg cagcgccagt agggtgagta gcgcccacac cggcaaggcg
      901 cccagcgcca cggccgccaa caaccacagg taagccagcg ccacgatcag cgcataaccc
      961 cagcgcgcct gcccgacctc cagccgtgcg acccagtgca gcttgcccgc cgccgtgtcg
     1021 gcgcggcgat ccgggaactg gttgatgtac agcagattgg tcaccagcag cgcataagac
     1081 aatccggcaa taaacggggc cgttgcgaac tctcgacgct gcacaaaatc cgtgcccacc
     1141 gcaatcagca ggaatcccgc cgccacgcac agctcgcccc agccgcgact gttcaacttg
     1201 aacggcgtcg cggaataggc ccagccgatg aacagcccgg ccagcccgat atagaacagc
     1261 tgtgcggcgg aatgcgccat cagccacagc cctgctgctg ccactgcggc catcaatgcg
     1321 aaaccgaagt tgcgcgtctg cccctgcgtc agcacgccgt tctggatgaa gcggctgccg
     1381 ccggtgaagg ggaagatgcg ctcgacattc tgcgcatcgg tgccgttgag tgcgtcgtag
     1441 taatcgttca gcacgttggt gccggcctgc gccatcaacg cgaacagcag cgtgaccacg
     1501 gccagttcgg cattgatgac gagaccgtcg tggcgcgcga tcgccagacc gatgcaacag
     1561 gccatcaggc tcgcggaaag aaaggccggg cgggtcgcag cgacatagcg cgcgaccggg
     1621 ttgcgcaaca cttcaagagt cggttccagc ggttgggtca taccattctt cctccacgca
     1681 cacgaaagcc cttggtcgcc gccagggtgg ccatgccgcc catgccgccg tgcgcatgac
     1741 agatcgcaca ggccagcgca tccgccgcat ccgggctggg cgtgccggac aatttcagca
     1801 ggcgaaccac catggcctgc acctgcgtct tgtcggcatg accgttaccc accacggcct
     1861 gcttcacctg caacgcggtg tattccgcca ccggcagatc ggccagcacc gcagcacaga
     1921 tcgccgcgcc gcgcgcctgt ccgagcaaca gggtggattg cggattgacg ttgacgaaca
     1981 ccttctcgac ggaggccaga tgaggccggt gcagctcgat cacttcgcgc aacgaatcga
     2041 gtatcacccg caagcgtccc ggcaactcgc cgtccggcgt cttgatgcat ccgctggcga
     2101 cgtagtgcaa ctgctgacct tccttgtcga tgaccccaaa acccgtgatg cgtaaacctg
     2161 ggtctatccc tagaattcgg attgaggatt gtggatttag gattgagcta ggcatcatga
     2221 cgcacccgtg cgatcttcct caatcctaat acccgaatca tcaatcctcg atcaccgccg
     2281 tcgtatagac ttcctgcaca tcgtccagat tttccagcgc atcgagcagc ttctgcatct
     2341 tcacggcatc gtcgccggtg aacaccactt cgttggcggg cttcatgatg acttcgccga
     2401 attctggctt gtagccggcg gcttccagcg cctgtttcac gttcacgaac tcgtacggtg
     2461 cggtgatgac ttcgatactg ccgtcgtcgt tggtggtgat atcttccgca cccgcatcca
     2521 gcgccacttc catcaggccg ttctcgtcgg ttcccggcgc gaagatcatc tggccgcagt
     2581 ggttgaacag aaacgccacc gaaccgtccg tgcccagatt gccgccatgc ttggaaaagg
     2641 cgtgacgcac atcacccacg gtgcgggtct tgttgtcggt catgcagtcc accatcaccg
     2701 cagcgccgtt gatgccgtag ccctcgtagc gcagctcctc gtagttcacg ccttcgagct
     2761 ggccgctgcc gcgcttgatc gcgttctcga cggtgtcctt gggcatgttg ttctcgcgcg
     2821 ccttctcgat cgccaggcgc agacgcggat tcgcccccgc atccccgccg cccatgcgcg
     2881 ctgcgacggt gatttccttg atcagccgcg taaagacctt gccgcgcttg gcgtcctgct
     2941 tgcctttgcg gtgttgaata ttcgcccact tgctgtgtcc tgccatcttg ctctccgaaa
     3001 aaacacctcg aacgacctgc tgcgcgaccg gattgcggcg ttgcgcggta ctcatctcct
     3061 aacctgccac agggcaggta tcgtcattcc gttccgtgcg ccttgcactc aggccgctcg
     3121 ctacggttgt tcgaggtgtt taattatcaa tgacgttcaa ataaaaaatc cccgcgatgg
     3181 tatcattttc gccccgttag cccaataaca agccattctg atacaccatg aacgaaccgc
     3241 tgctgattgc caagaacgac caacatgaac tctgcctgct gccgcagatg gcgaaccgcc
     3301 acggcctgat caccggcgcg accggcaccg gcaagaccgt cacgctgcag acgctggccg
     3361 aatcgttcag ccgcatcggc gtgccggtgt tcctgtccga catcaagggc gacctgtccg
     3421 ggctctccaa ggcgggcggc ggcaatgcca aggtcgcggc gcgcgtacag cagctcggac
     3481 ttagcgactt tacccaccgc gcctacccgg tgacattctg ggacgtgttc ggcaagatgg
     3541 ggcatccgct gcgcgccacg gtctccgaca tggggccgct gctgctcggg cgcatgctga
     3601 acctgaacga ggtgcaacag ggtgtgctgg cgctggtgtt caagatcgcc gacgacaacg
     3661 gcctgctgct gctcgacctg aaagacctgc gcgcgatggt gcagcatgtc ggcgagaacg
     3721 ccggcgactt caccaccgaa tacggcaaca tctccaccgc cagcgtcggc gcgatccagc
     3781 gcggcctgtt gcagatcgag acgcagggcg gcgacaagct gttcggcgag ccgatgctga
     3841 acatcgaaga cctgatgcag accgatgcca acggcctggg catgatcaat attctcgccg
     3901 ccgaccagct gatgacctcg cccaaggtgt attccacgat gctgctgtgg ctgctggcag
     3961 agctgttcga gaacctgccg gaggcgggcg atctggccaa gccgaagatg gtgttcttct
     4021 tcgacgaggc gcatctgctg ttcaccgatg cgcccgccgt gctgctcgac aagaacgaac
     4081 aggtggtgcg cctgatccgc tccaaggggg tgggcgtgta cttcgtcacc cagaacccgg
     4141 cggacgtgcc ggacaaggtg ctgggacagc tgggcaaccg cgtgcagcac gcgctgcgcg
     4201 ccttcagccc gcgcgaccag aaggcggtga aagccgccgc cgagaccatg cgcgacaatc
     4261 ccgaactgga tgaagccgcc gccatcaccg agctcggcgt cggcgaggcg ctggtctcct
     4321 gtctggacga gcatggacgt ccgggcatcg tcgagcgcgc cttcgtcgtg ccgccgcagg
     4381 cgcagatcgg cccgatcgat accgctgagc gcagcgccgt gatcgccggc tcgctgctcg
     4441 ccggacacta cgagaaggcg gtggatcgcg aatccgccta cgaaatcctc aagggccgca
     4501 ccgccgagaa acaagccgct gcgagcacgg cgcctcgcgg cgaatccggc ggcgtgggcg
     4561 gcatgctggg cggcggcagc cgccgcgaag gcgcagtgga agcgctggcc aagagcgccg
     4621 cgcgcgccat cggctcggag gtgggacgtc agatcatccg gggcgtgtta ggctcgctgc
     4681 tgggcggcag acgttaacct aacggcgcag ctatcaacca aggaaggcgg gcatgaaaac
     4741 catactggta gcgaacccca aaggcggcag cggcaagtcc acgctgtcgg tcaacatcgc
     4801 gggctatctg gccaatcgcg gccagcatgt cgccatgctc gatctcgacc gccagcaatc
     4861 ctccgcgctg tggctgtcca tgcgttcgga cgaactgcct ccgatacact cgctcgatcc
     4921 caagaagaaa gacgaccgcg catacgactg gctggtgatc gactcgcccg ccggcctgca
     4981 cggcaagaat ctcgcccatg cgctgagact ggccaacaag atcatcgtac cggtcgcccc
     5041 ttccctgttc gatctgcagg ccagccagga ttttttgcgg ctgctgggcg aagaaaaagc
     5101 aggacgcaag ggcaagtgtc acatcggggt ggtcggcatg cgcatggatg ggcgcacccg
     5161 cgcggctcag gcactggagc attttttgtc cagtctcgat ctgccggtat tggcgtacct
     5221 gcgcgaaacg caggtatacg tgaatgccgc cttcgaagga aaatcgctgt tcgacctgcc
     5281 gccctatcta gccgagcgcg aactggagca atggacctac ctgctggact ggctggagta
     5341 gttaaccctg cggcgcctga aagtcaggca gggtttttgc cttgtccgcg atcccaccgc
     5401 gcttgagcat ggtgaaatgt tcggcctctt ccagctgggt gactcgcgcc agataatcga
     5461 tattcttctc gatcgactcc aggtagtagt tgttccccac ccccatggtc accctgaaat
     5521 gggctgacag ccgggcgtac accccaccat agacgacatc cagcttttca cgggtgagat
     5581 cgtagaaacg cttggtctgg cgcaacatgt cgtgcacatt ttgatagtta ccgaaatgcg
     5641 cttccttgaa ttccagatag aggaacagca gcttctccag ataggtgcga tcggccatct
     5701 gcccgaccag atctgccgtc gcgacaagct ggcccagcag gcgctggtgc tcatccgcga
     5761 aggcgatctg cgcaaaggga aagctggggt cggtggcacg gatcagcggc tcgatccttg
     5821 tcgcaaggtc agccggccag cctttcgccg cgagataatt gtgcatgaac tcgatgccgc
     5881 gcgagacatg acacaacgtg tactgggctc cggtaccggc ctcttcattg ctggcctgcg
     5941 cataaccgat gtcgtgcatc agcgccgccg tcatgaccag cacgacatcc tcgtcgcgca
     6001 gcggcgtgcc ggagacatgc acaccgtgca tcaggcgcac cgcacacaga aacacatgca
     6061 gcgtatgcgc cagatcgtga tacagcgtct tgacgggggc ataggccgga tattcgccat
     6121 ggaacagcgc gaccatatcg tcgaagacgg cacgcatagg cgcaaggtcg cagtcaggat
     6181 tgatcagcag gatgatgctg gccgccttgt cccacacctc gttcgcatcg ctgttggcga
     6241 acaaactgtt cacctcctca gaaaacggaa ccatcttgct agccatttcc gccaccccct
     6301 ttcaggcccc tcggccgggt tacacgttga acaggaagtt catcacgtcg ccgtcctgaa
     6361 ccacgtactc cttgccttcc acacgcatct tgcctttttc cttggccccc gcctcgccgc
     6421 cgcaggcgat gaaatccgca taggaaatag tctgcgcgcg gatgaagccc ttctcgaaat
     6481 cggtatggat cacgcccgcc gcctgcggtg cggtgtcgcc cttgtggatg gtccaggcgc
     6541 gcacttcctt cacaccggcg gtaaagtagg tctgcagacc gagcagttcg taaccggtgc
     6601 ggatcagctt gttcagaccg ggctcgtcca gccccagatc ggccagaaac tcctgcttgt
     6661 cggcatcatc cagatcggcc agttcggcct cgatcttggc acacagcgcg acgaccggcg
     6721 caccctcttt cgcggcgtgc tcacgcagac gatccagatg gggattgttc tcgaagccgt
     6781 cttccagcac gttgccgaca tacatcgcgg gcttgatggt gagcaggcac agcggcttga
     6841 gcaacagctt gtcatcgtcg gtcagcggga aggtgcgcgc aggcttgcct tcattcagat
     6901 gaggcagcag tttttccagc agggccacca gcttctgtgc atccttgtcg cctgacttgg
     6961 ctttcttgcc gtcacgctga atagtgcgct ccaccaccgc catgtcggcc agcgccagtt
     7021 cggtgaggat gacctcgata tcggacagcg gatcgacctt gccggccaca tgtacgacgt
     7081 tctcgtcatc gaaacagcgc accacgttga cgatggcatc ggtctcacgg atgttggcga
     7141 ggaactggtt gcccagaccc tcgcccttgg aggcccctgc gaccagaccg gcgatatcga
     7201 cgaactcgac gatggcgtgc tgcatcttct gcggcttgac gatcttcgcc agttcttcga
     7261 ggcgcggatc gggcacctcg acgatgccga cgttcggctc gatggtgcag aagggatagt
     7321 tctccgccgc gatgcccgac ttggtcagag cgttgaacag ggtggacttg ccgacgttgg
     7381 gcagaccg
//
LOCUS       DASUIV010000087         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_764136, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000087 DASUIV010000000
VERSION     DASUIV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764136"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFW59_03265"
     CDS             <1..1107
                     /locus_tag="VFW59_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5363264.1"
                     /translation="TLMMKPELIDTLITSAYDAVLHEASWAPVLERLAVLTGSNSCAY
                     VTIDGPLPEIVEEYGFDPAVTDEYNRHYAAFDPFHGAILRKPRSLPLIDRQVVSADFI
                     RQSSWFQDFMPRHQLHTMLVLPLFRDGELVGSFNTQKFCGQGDFSADEFAAMQAVTPH
                     LIKAAQLSTKMSGLRRSVAYSEAMLDRLGLPLLLTDEAGKVLLVNRAAELLIAREPAL
                     RLQQGTFSVHGSDGTRTPRRSAHIVEMSIPRGGARKPLRLTMLPVSPHSAVNSLWQRP
                     MMMIVIDDPESIPVGLNEILQGLFGMTAAEARVCVALACEGLSPQSCADVFGVSITTI
                     RSQIRSIYSKTGVKRQMELVRMLTMLGATRGTGR"
     gene            complement(1482..1856)
                     /locus_tag="VFW59_03270"
     CDS             complement(1482..1856)
                     /locus_tag="VFW59_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX5363265.1"
                     /translation="MKTSAPVLRDYLGHIRDAIERINRYTALMDEQEFLSSELTQDAV
                     LRNFEVIGEVSSRILKDHKALADAHPELPLMIAYQMRNAIAHGYFSVDHQIVWRTIKM
                     DLTVLLDQLRHLLNAATGPLTQ"
     gene            complement(1853..2143)
                     /locus_tag="VFW59_03275"
     CDS             complement(1853..2143)
                     /locus_tag="VFW59_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5363266.1"
                     /translation="MKPSEALRLHRQAIRKIVESHRARNARVFGSTARGEDEEGSDLD
                     LLIEPTPETTLFDIGAIRHELLELLGVPVDVLTPNALPEKLRAQVIAEAVSV"
     gene            2218..>3350
                     /gene="pdxH"
                     /locus_tag="VFW59_03280"
     CDS             2218..>3350
                     /gene="pdxH"
                     /locus_tag="VFW59_03280"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX5363267.1"
                     /translation="MYDTAPDFSQPIAVLKHCHDRIRKQLQTLQNLLGHLPKHGADSE
                     AQKAAQAVLKYFNHAAHLHHEDEEQNLIPMLQATARDADAVLLAELVPGILAGHEQMD
                     KDWSIIKSQLEQIANGASSALSASDVARFCDTYAAHMVVEENNIAPMAKRLFSAEQMA
                     QLGSAMQVRRGITPDLPISQVPEQQAVPVNNTVADGVVLADLRLDYGRASLTEHDVLD
                     DPIAQFGKWFEEALHAKVNEPNAMSVATVDADGKPSSRIVLIKQYDHRGFTWYTNYES
                     QKGRQLRANPHAALLFFWSELERQIRIEGKVVQTSAEESDKYFYSRPVKSQIAAIASQ
                     QSAPIANRELMETNYDAAAAASGEHPLRPAHWGGYRLDPERIE"
BASE COUNT          725 a          994 c          981 g          650 t
ORIGIN      
        1 acattgatga tgaagcctga attaatcgac acgctgatca catcggccta cgacgccgtc
       61 ttgcatgagg caagttgggc gccggtactg gaacggctgg ccgtgctgac cggttcaaat
      121 agttgtgcct atgtgacaat cgatggtccc ttgccggaaa tcgtcgagga atacggattc
      181 gatcccgccg tcacggatga atacaaccgg cactacgcag cgtttgatcc attccatggc
      241 gcgatcctgc gtaagccgcg cagtttgccc ctgatcgacc gacaggtggt cagtgcggac
      301 ttcatcaggc aaagcagctg gtttcaggat ttcatgccgc gccaccagct gcacaccatg
      361 ctcgtgttgc cccttttccg cgatggcgaa ctagtgggat cgttcaacac gcagaaattt
      421 tgcgggcaag gcgatttcag cgccgacgag ttcgctgcca tgcaggcggt aaccccccac
      481 ctgatcaagg cagcgcagtt gagtacgaag atgagcgggc tgcggcgatc cgtcgcctac
      541 agcgaggcga tgctcgacag actcggactg cccttgctgt tgacagacga ggccggaaag
      601 gtcctgctgg tcaatcgggc ggcggagcta ttgatcgcac gcgagccggc attgcgcctg
      661 caacagggaa ccttcagcgt tcacggcagc gatggcacac ggacaccacg ccggtcggcc
      721 catattgtcg agatgtcgat cccacgcggc ggcgcgcgca agccgttgcg cctcaccatg
      781 ttgccagtat cgccgcatag cgccgtcaac agcctctggc agaggccgat gatgatgatt
      841 gtgatcgacg atcccgagag catccctgtg gggctgaacg aaatcttgca aggtttattc
      901 ggcatgaccg cggcggaagc acgcgtctgc gtggcgctgg cgtgtgaagg actgtcaccg
      961 cagtcctgcg ctgacgtctt tggcgtcagt attacgacca ttcgctccca gattcgctcc
     1021 atttattcca agactggtgt caaacgccag atggagctgg tgcgaatgct gacaatgctg
     1081 ggagcaaccc gaggtactgg gcggtaaaaa agagactgcg tgtgggcgca gtattcccat
     1141 gagtgaatct aagaaccggt gaaagccgcc ttcaccaagt tccagccaaa gtggattccg
     1201 gtcgatgggg tcggggcaag caccatgccc gctctagatg cactggatat tcaaaaactc
     1261 gaaatgggtc gctccaggcc gagcgtgaaa atcagtgagt tttgcagccg agcaagctgc
     1321 tatcctgaac gccgtcgccc gcgttcggcc aatagcggct gttccgtcat tcccgcgaag
     1381 gcgggaatcc atacttcgcc ggcacactgc cgacctatgg attccggcct acgccggaat
     1441 gacgggagca aggcgaccgg cggcaacggg gcgaaatcgg actattgtgt caacggaccg
     1501 gttgcggcat ttagcagatg ccttagctga tcaagcaaca ctgtaagatc catttttatc
     1561 gtacgccaaa ctatttgatg gtcaacgcta aaatagccgt gtgcgatagc gttgcgcatt
     1621 tggtaggcga tcattagcgg tagttctgga tgagcgtcgg ccaatgcctt gtgatctttg
     1681 agaatacggc tactcacctc accgatgact tcaaagttgc gcagcactgc gtcttgggtc
     1741 agttcgctac tcaggaattc ttgttcatcc atgagcgcgg tgtaacgatt aatccgttca
     1801 atcgcatcgc ggatatgtcc gaggtaatct ctcagtacgg gagcggacgt tttcataccg
     1861 acaccgcctc agctatcacc tgggcgcgta atttttccgg caatgcgttc ggtgtcagca
     1921 cgtccactgg cacgccaagc aactcaagca attcatggcg gatggcgcca atgtcgaaca
     1981 gcgtggtttc cggtgttggc tcgatcagga gatcgaggtc gctgccctcc tcgtcttcgc
     2041 cgcgcgcggt cgagccgaag acacgtgcgt tcctcgcgcg gtgcgattcc acgatctttc
     2101 ggatggcttg ccggtgcagg cgcaaggcct ccgagggctt cactaaagtc tcccaaatgc
     2161 gatttactct agaatactcc tgttaccagc ccaagccctc tcgccatgag caatactttg
     2221 tacgataccg cccctgattt cagccagccg atcgcggtgc taaaacattg ccacgaccgc
     2281 atccgcaagc agctgcaaac cctgcagaac ctgctcggcc acctgcccaa gcacggcgcc
     2341 gatagcgagg cgcagaaagc cgcgcaggcg gtgctcaagt atttcaacca cgccgcccac
     2401 ctgcaccacg aggacgagga gcaaaacctg atcccgatgc tgcaggccac cgcgcgcgac
     2461 gccgatgccg tccttttggc agagctggtc cccggcattc tggccggcca tgagcagatg
     2521 gataaagact ggtctataat caaatcgcaa cttgagcaga ttgccaacgg cgccagttcc
     2581 gccctctcgg ccagcgatgt cgcgcgcttt tgtgacacct acgcggccca catggtagtg
     2641 gaagaaaaca atatcgcgcc gatggccaag cgcctgttca gcgccgagca aatggcgcag
     2701 ctgggcagcg cgatgcaggt gcggcgcggg attacccccg acctgccgat cagccaggtg
     2761 cccgaacaac aagccgtccc ggtcaacaac acggtggccg acggcgtggt gctggcggac
     2821 ttgcgcctcg attacggccg cgccagcctg acggaacatg acgtgctgga cgatccgatc
     2881 gcccagttcg gcaaatggtt tgaggaagcg ctgcacgcca aggtcaatga accgaacgcg
     2941 atgagcgtgg cgacggtgga cgcggacggc aagccaagct cgcgcatcgt gctgatcaag
     3001 caatacgacc accggggctt cacctggtac accaattacg agagccagaa aggccggcaa
     3061 ctgcgcgcca atccgcacgc cgcgttgcta ttcttttgga gtgagttgga acgccagatc
     3121 cgcatcgaag gcaaggtggt gcagacctcc gccgaggaaa gcgacaagta tttttacagc
     3181 cggccggtga agagccagat cgctgcgatc gcttcgcagc agagtgcgcc catcgccaac
     3241 cgcgagctga tggaaaccaa ttacgatgcc gccgcagctg ccagcggcga acatccgctg
     3301 cggccggcgc actggggcgg ttaccgcctc gacccggagc gcatcgagtt
//
LOCUS       DASUIV010000088        10394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_577892, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000088 DASUIV010000000
VERSION     DASUIV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 10394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10394
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577892"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /gene="rseP"
                     /locus_tag="VFW59_03285"
     CDS             <1..1077
                     /gene="rseP"
                     /locus_tag="VFW59_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX5363268.1"
                     /translation="RMAIVAAGPLANFLLAILLYWVMFIHGVPGLKPVLGEVPSGTPA
                     AVAQMQSGETILSVDGMLIPNWQELRWTLLDLALQQSEVRIEARSAEGEPLLHVLDLR
                     GLKASDLDGEFFDKLGLHLYRPTILPVIGKLTADGVAQQAGLREGDMVLRANGIALHR
                     WDELVEVVRTHPGQAVLLDIRRGELALSITLVPKTVEEGGVSVGKIGAAPQVDKGAWK
                     AMQNEVSYNPVTALTLALRKTWETSVVSLKMMGKMVIGEISVKNLSGPLAIADYAGQS
                     AEMGVAAYLGFLALISISLGVLNLLPIPLLDGGHLLYYVAEMVKGSPVSEQAWEFGQK
                     IGVALLGTLMVFAIYNDVTRLISG"
     gene            1080..3356
                     /gene="bamA"
                     /locus_tag="VFW59_03290"
     CDS             1080..3356
                     /gene="bamA"
                     /locus_tag="VFW59_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX5363269.1"
                     /translation="MRLKKLAGLISLLCMSNAWSIEPFTVKDIRVEGIQRTEAGTVFS
                     YLPVKVGDKMDDAQAASAIRALYATGFFKDVRLEVEQGVLIVLVRERPSIASIEVNGI
                     KSFPKDQIKENMKYAGLAEARIFDKGALDKAIQELKKRYVAYGKYGVSVTSTVTELER
                     NRVGIVFNVVEGDVSKIKQINLVGNHAYGEDELLEMMKLSTPNWMSWMSKNDQYSKQK
                     LSADMEVLRSFYMDNGYLEFSIDSTQVSITPDKKDIYITVNFTEGAKYTVSKVEVKGN
                     TVVPREEIEKLVQVKPGDTFSRRALTETSKKVGERLGQEGYAFANINAIPDIDKEKHE
                     VAFNFVVDPGQRVYIRRINIAGNDKTRDEVIRREFRMVEGAWFDVDKIKRSKQRVDKL
                     NFFDEVNLETPQVQGAGDQLDVNVAVHEKSTGNFSVGAGFSQGEGITFTTGVTQSNLF
                     GSGNYAAVNVNTSKINQVYSFSYTNPYYTDEGVSRGFDVYKRNTDLTGTAVSQYGTHT
                     LGGGVRFGVPIAEEESINYGLSAENTKLILGTNPPARMTQYVNTFGASNTNLMGTVGW
                     SRDTRDSAIYTTEGMVQNAFLELALPVFDMRYYKLNYQQQFFYPVSSNVTLLMNGEAG
                     VAGGYGNKPLPFFKNFYAGGTGSVRGYQSNSLGPRDVNNQALGGTRRMVGNAELMVPV
                     PGLMKEKSVRLSGFVDAGAVYGPGDLPGSAGLRYSTGAAFTWISPMGPIKFSYAVPLN
                     KQQQDLVQKFQFTIGSMF"
     gene            3433..3954
                     /locus_tag="VFW59_03295"
     CDS             3433..3954
                     /locus_tag="VFW59_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX5363270.1"
                     /translation="MKEMIKAAMLALLLGIGAAQAGDFKVGVVDTERVLRESAPAIKA
                     EKRIEKEFSGRDQEIKKLMKQAKELQTMLEKDAGSLSDADHRNKERELTAMNVNLQRM
                     QREFREDLNLRKNEELAIVLEQANKAVQAIAEAEKYDLILQEAVYRNAKIDITEKVIK
                     YLAIDRATAKDGK"
     gene            3961..4995
                     /gene="lpxD"
                     /locus_tag="VFW59_03300"
     CDS             3961..4995
                     /gene="lpxD"
                     /locus_tag="VFW59_03300"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX5363271.1"
                     /translation="MRATHKLSAIAAQFGGRVLGDGEVQVEQIATLDSAQPQHISFLT
                     NSKYRSQLAGTKAGAVILAEADADATDLPRIICANPYAYFAKVSALLNPLPEASPGVH
                     PTAVIGAGARIDPTASIAATAVVGAGAVIGAYSVIGEGCCIGDNVVIGCRARLYPRVV
                     VYHDCVIGNDLIAHSGCVIGSDGFGIAMDEGRWIKIQQIGRVVIGDNVEIGANTTIDR
                     GALNDTVIEEGVKLDNQIQVAHNVRIGAHTAIAGCVGIAGSTTIGKYCRIGGSAGILG
                     HLQIADHVEISSFTLIGKSIKESGSYSGIFPFSRNEDWRKNAVHLRHLDEMAKRLKAL
                     EHQIESLKHP"
     gene            5009..5458
                     /gene="fabZ"
                     /locus_tag="VFW59_03305"
     CDS             5009..5458
                     /gene="fabZ"
                     /locus_tag="VFW59_03305"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5363272.1"
                     /translation="MSTTMDIHAILESLPHRYPFLLVDRVLSLEPGKNIVALKNVTIN
                     EPFFPGHYPHHPVMPGVLIIEAMAQVAALLSFQSMESKPDEKSVYYFAGIDNARFKRP
                     VTPGDQLIFKVELTRSMRGVYKFKAIAEVDGQLAAEAELMCTVKTVA"
     gene            5481..6251
                     /gene="lpxA"
                     /locus_tag="VFW59_03310"
     CDS             5481..6251
                     /gene="lpxA"
                     /locus_tag="VFW59_03310"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX5363273.1"
                     /translation="MIHPTAIVHPNAKLAADVEVGAYSIIGEHVEIAEGTVIGPHVVI
                     NGHTRIGRHNRIFQFCSLGEIPQDKKYANEPTRLEIGDHNTIREFCTFNLGTAQDVGV
                     TRVGNHNWIMAYVHLAHDCQVGNNTIFANNSQLAGHVHVGDYAILGGFTVVHQFVRIG
                     EHIITGMGTILFKDVPPYVLASGNPCAPHGINSEGLKRRGFSSEAIMSIKRAYKTLYK
                     SGLSLDEAKAAIDAQLAEHAELQLLADFLATSTRGIVR"
     gene            6268..7419
                     /gene="lpxB"
                     /locus_tag="VFW59_03315"
     CDS             6268..7419
                     /gene="lpxB"
                     /locus_tag="VFW59_03315"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX5363274.1"
                     /translation="MQQKKIVIGIVAGEASGDMLGSHLMAALKQARPDIHFVGIGGPK
                     MQSVGMEVLFPMEKLAVRGYVEVLRHYREIVGIRRKLRAHFLKQRPDLFIAVDAPDFN
                     LDLELVLKANGIPTIHYVSPSIWAWRKERIHKIKRAVSHMLALFPHEPRLYQQAGIPV
                     DYVGHPLADMLPDYPKRNEMRETMRIPLQARVFAFLPGSRQSELKQLARTYIETAKLM
                     LQQFPDARFLVPLVSRETRGIFEQALYDCDAEQLPITLLFGHAQDAMIAADGVLVASG
                     TATLECALLKRPMVITYKMPALTWWLMKRKSYQPYYGLPNILCERFVVPELIQEDATP
                     ENLAQALLNLVNDKDVVAKIEETFLDLHRTLRQNTAQKAAAAILPYLPA"
     gene            7416..8009
                     /gene="rnhB"
                     /locus_tag="VFW59_03320"
     CDS             7416..8009
                     /gene="rnhB"
                     /locus_tag="VFW59_03320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX5363275.1"
                     /translation="MSAIILVCGVDEAGRGPLAGPVSAAAVILDPAYPIEGLADSKKL
                     SERKRDLLAPVIRERALAWAVAYAEVEEIDSLNILQATLLAMRRAVLALPIQPQHVLV
                     DGLYCPQTGIPSEAIVKGDSKVAAISAASILAKTARDELMLKMHEQFPHYGFAEHKGY
                     PTAAHLAALREHGVSAVHRKSFKPVRELLQYTRQNNE"
     gene            8017..8472
                     /locus_tag="VFW59_03325"
     CDS             8017..8472
                     /locus_tag="VFW59_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5363276.1"
                     /translation="MSLFKLIHLLAVLVWVGGMFFAYVVLRPAAVEVLQPPERLRLWD
                     SLFRRFFNWVWLAIGVLLVSGFYMVYLYGGLASVPRHVHIMLALGLVMMAVYGYVFFA
                     CYVPLSLHVAKQRWAEAGEQLGRIRKLVAFNLTLGLLTVSVAVIGVALS"
     gene            8525..8848
                     /locus_tag="VFW59_03330"
     CDS             8525..8848
                     /locus_tag="VFW59_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5363277.1"
                     /translation="MNCGACCTNFRVSFYWAESDDALGGLVPAHLTENISPHLRCMKG
                     TTGKPVRCIALEGEVGVSVSCGIYPLRSSTCREFDVFEADGSINARCNSLRIGLGLDP
                     IDLSL"
     gene            8946..>10394
                     /locus_tag="VFW59_03335"
     CDS             8946..>10394
                     /locus_tag="VFW59_03335"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX5363278.1"
                     /translation="MDNIGTSAKQVFAFEEGDGKNKKLLGGKGANLCEMTQIGLNVPP
                     GFVISTEACLSYLAASGEVLPKSLLDEINAQMKALEAKTGKRFGDAAAPLLVSVRSGS
                     AMSMPGMMDTILNLGLNPKTLAGEIAQTGDPRFGYDTYRRFIQLFGKIALDISDELFD
                     AELNAVKQDRGIKEDLALTADDLKEISERFLRVVQEQTGRAFPEDPYEQLILSVKAVF
                     RSWMGKRAVDYRREFHITKEMANGTAVNICTMVFGNRGNDSATGVGFTRNPATGENHI
                     FGEYLVNAQGEDVVAGIRTPKPIDYMATEMPDLFRQLQELRAKLESHYHEVQDFEFTI
                     ERGTLYCLQTRNGKMNTTAMVRTSVEMEKEGLITRRQALLRIAPDSLAQMLFPRLDPA
                     SKVTPLAKGLPASPGADYGHAVFDADRAEEQGRAGKPVILVREETKPEDIHGFFASRG
                     ILTSRGGKTSHAAVVARGMGKPCVAGAEGIEVN"
BASE COUNT         2195 a         2794 c         3228 g         2177 t
ORIGIN      
        1 cgcatggcca tcgtggcggc agggccgctg gcgaattttt tattggctat cctgctgtac
       61 tgggtgatgt tcatccatgg ggttccggga ctgaagccgg tgctgggcga ggtgccgtcg
      121 ggcacccccg ccgcagtagc gcagatgcaa tccggcgaaa ctatactcag cgtcgatggc
      181 atgcttattc caaattggca ggagttgcgc tggacgctgc tggatcttgc gttgcaacag
      241 tccgaggtgc ggatcgaggc gcgtagcgcc gagggtgaac cgttgttgca tgtgctcgat
      301 ttgcgcggat tgaaggccag cgatctggac ggtgaatttt tcgacaagct gggtctgcat
      361 ctgtatcggc ctaccattct gcctgtcatc ggcaagctca ccgcagacgg tgtcgcgcag
      421 caggccggtt tgcgggaggg tgatatggtc ctgcgtgcca acgggattgc gctccatcgt
      481 tgggacgaac tggtcgaggt ggtgcgcact catcccgggc aagctgtgct gctcgacatc
      541 cggcgcggcg aattggcgct gagtattacc ctggtgccca agaccgtgga ggagggtggc
      601 gtgtcggtcg gcaagatcgg tgcggcaccg caagtggata aaggggcctg gaaggcgatg
      661 cagaacgagg tgagctataa cccggtcact gcgttgactc tggcgttgcg caagacctgg
      721 gagacatcgg tcgtcagcct gaagatgatg ggcaagatgg tgatcggcga aatatcggtg
      781 aagaacctga gcggccccct tgccatcgcg gattatgccg ggcaatccgc cgaaatgggt
      841 gtcgccgctt atctgggatt tctcgcgttg atcagcatca gtctgggcgt gttgaatctg
      901 ctgcccatcc ccttattgga tggcgggcac ttgctgtatt atgtcgccga gatggtgaag
      961 ggaagtcctg tctcggaaca ggcatgggag tttggccaga agatcggtgt cgcactgctc
     1021 ggaacgctga tggtgtttgc catatataac gatgtaactc ggcttatttc aggttaaaaa
     1081 tgagattaaa aaaactggcg gggctgattt ccctgctttg catgtcaaac gcttggtcga
     1141 ttgaaccatt caccgtcaaa gatattcgcg tagaaggtat tcagcgtacc gaagccggta
     1201 cggttttcag ctatctcccc gtcaaggtcg gcgacaagat ggatgatgcg caggcggcct
     1261 cggcgattcg tgccctgtat gccaccggct tcttcaagga tgtgcgtctg gaagtcgagc
     1321 agggcgtgtt gatcgtgctg gtgcgggagc gcccttcgat cgccagcatc gaggtcaacg
     1381 gcatcaagag ttttcccaag gaccagatca aggaaaacat gaaatatgcc gggctggccg
     1441 aggcgcggat tttcgacaag ggagcgctgg acaaggcgat acaggaactg aagaaacgtt
     1501 atgtggccta tggaaaatat ggcgtgagcg taacctccac ggtgacggag ctcgaacgca
     1561 accgtgtcgg catcgtgttc aacgtggtgg aaggcgatgt ctccaagatc aagcagatca
     1621 atctggtcgg caaccatgcc tatggcgaag acgaactgct ggagatgatg aagctcagca
     1681 cgcctaactg gatgagctgg atgagcaaga acgatcagta ttccaagcag aaactgtctg
     1741 cggacatgga agtgttgcgt tcgttctaca tggataacgg ttatctggag ttcagcatcg
     1801 attccaccca ggtctcgatc acccctgaca agaaagacat ctacatcacc gtcaatttta
     1861 ccgagggcgc gaaatatacc gtctccaagg tggaagtgaa gggcaatacc gtggtgccca
     1921 gggaggagat cgagaagctg gtgcaggtca agccgggtga taccttttcc cgccgcgcgc
     1981 tgaccgagac gagcaagaaa gtcggcgagc gtctggggca agagggttat gccttcgcca
     2041 acatcaatgc gatacccgat atcgacaagg aaaagcatga ggtggcattc aacttcgtgg
     2101 tcgatccagg gcagcgcgtg tatatccgcc gcatcaacat cgccggcaac gacaagaccc
     2161 gcgacgaggt gatacgccgc gagttccgca tggtcgaggg ggcctggttt gacgtggaca
     2221 agatcaagcg ttccaagcaa cgcgtggata agctaaactt cttcgatgag gtgaatctgg
     2281 aaacgcctca ggttcagggg gcgggagacc agctggacgt caacgtcgcc gtccacgaaa
     2341 aatccaccgg aaacttctcg gtcggcgcgg gcttttctca gggtgagggc atcaccttta
     2401 ccacgggtgt cacccagtcg aatctgttcg gtagcggtaa ttatgcggcg gtgaatgtca
     2461 acaccagcaa gatcaaccag gtctattcgt tctcctacac gaatccctat tacaccgatg
     2521 aaggcgtgag tcgcggtttc gatgtctata agcgcaacac cgatctgact ggaaccgcag
     2581 tcagtcagta tggtacccat accttgggcg gcggagtgcg gttcggggtg cctatcgcag
     2641 aggaggagtc gatcaactac ggcctgtcgg ccgagaatac caaattgatt ctgggcacga
     2701 atccgcccgc tcgtatgacc cagtatgtca acacgttcgg cgcctccaat accaatttga
     2761 tgggcacggt cggctggtcc cgggatactc gtgacagcgc gatctacacc accgagggca
     2821 tggtgcagaa cgccttcctc gaattggcct tgcctgtgtt cgatatgcgc tattacaagc
     2881 tgaactacca gcagcagttc ttctatccgg tgagcagtaa tgtcactctg ttgatgaatg
     2941 gcgaggccgg tgttgccgga ggatatggca acaagccgtt gccgttcttc aagaacttct
     3001 acgctggtgg taccgggtcg gtgcgcggct accagtcaaa ttcgctgggt ccgcgtgatg
     3061 taaacaatca ggccttgggc ggcacgcgtc gtatggtcgg caatgccgaa ttgatggtgc
     3121 cggtcccggg gttgatgaaa gaaaaatcgg tgcgattgag cgggttcgtc gacgcgggtg
     3181 cggtgtatgg cccgggagac ttgccgggca gtgcggggct gcgttattcc accggcgcgg
     3241 cattcacctg gatctccccg atggggccga tcaagttcag ctatgcggtg ccgctcaaca
     3301 aacagcagca ggatctggtg cagaagttcc agttcaccat cggttcgatg ttctaaataa
     3361 aaaagttcac cgcttgagct ggacaggttc tgctcgatgg gtgtgatagc gatgtatctt
     3421 caggagtgcg tgatgaaaga gatgattaaa gcagcgatgt tggccttgtt gttaggcata
     3481 ggggcggctc aggctggcga tttcaaggta ggggtggttg ataccgagcg cgtgctgcgc
     3541 gaatcggccc cggctatcaa ggcagagaag cgtatcgaga aggagttctc cgggcgcgat
     3601 caggaaatca agaaactgat gaagcaggcc aaggaattgc agaccatgct ggagaaggat
     3661 gccggttcgt tatcggatgc ggatcatcgc aacaaggaac gcgaattgac cgcgatgaac
     3721 gtgaatctgc agcgtatgca acgcgaattc cgcgaggatc ttaacctgcg caagaacgaa
     3781 gagctggcca tcgtgctgga gcaggctaac aaggcggttc aggccattgc agaggcggaa
     3841 aaatatgacc tgatcctgca ggaagccgtg tatcgcaatg ccaagatcga catcaccgaa
     3901 aaagtgatca agtacctcgc cattgacagg gcgactgcca aagacggcaa gtaagacgcg
     3961 atgagggcga cacataagct ttctgccatc gccgcgcaat tcggcgggcg tgtgctgggt
     4021 gatggtgagg tgcaggtcga acagattgcg acgctggata gcgcacagcc acagcacatc
     4081 agttttctga ccaacagcaa ataccgttcc cagcttgccg gcacgaaagc gggcgcggtg
     4141 atactcgccg aggcggatgc cgacgcgacc gatctgccgc gcatcatttg cgcgaatccc
     4201 tatgcctatt ttgccaaggt gtcggcattg ctcaatccct tgccggaagc gagcccggga
     4261 gtgcatccca ctgcggtgat cggggcggga gcgcggatcg atccgaccgc tagcatcgcc
     4321 gcgaccgcag tggtcggtgc gggtgcggtg atcggtgcct attcggtgat cggcgagggc
     4381 tgctgtatcg gcgacaatgt ggtcatcggt tgtcgtgcga ggttgtatcc gcgcgtggtg
     4441 gtctatcacg actgcgtgat cggcaatgac ctgatcgcgc attccggctg cgtgatcggc
     4501 tcggatggtt ttggcatcgc gatggacgag gggcgctgga tcaagattca gcagatcggt
     4561 cgcgtggtga tcggtgacaa tgtcgagatc ggggcgaata ccacgatcga tcgcggtgcg
     4621 ttgaacgata ccgtgatcga agaaggcgtg aagctggaca atcagattca ggttgcgcat
     4681 aacgtgcgca tcggcgcgca taccgcgatt gccgggtgtg tgggcattgc gggcagtacc
     4741 accatcggca aatactgccg tatcggcgga agcgccggca tcctggggca tctgcagatc
     4801 gccgatcatg tcgagatatc ttcgttcacg ctgataggga agtccatcaa ggagtccggc
     4861 agttatagcg ggatttttcc tttcagccgc aacgaggact ggcgcaaaaa tgccgtgcac
     4921 ctgcgtcacc tcgacgagat ggcgaagcgc ctcaaggcac tggaacatca gatcgaatca
     4981 ttaaaacatc cttgaaaagg aaacaagcat gagcaccacc atggacatcc acgccattct
     5041 cgagagtctg ccgcaccgtt atcccttcct gctggtagac cgtgtgctgt cgctcgaacc
     5101 cggcaagaac atcgtcgcgc tgaagaatgt caccatcaat gaaccgttct ttcccggtca
     5161 ttaccctcat catccggtca tgcccggcgt gctgatcatc gaggcgatgg cgcaggtcgc
     5221 cgcactgctg tctttccagt ccatggaaag caagccggac gagaagtcgg tgtactactt
     5281 tgccggcatc gataacgcgc gcttcaagcg cccggtcaca cccggcgacc agctgatctt
     5341 taaagtagag ctgacacgca gcatgcgcgg cgtctataag ttcaaggcga tcgccgaggt
     5401 ggacgggcaa ctggctgccg aagcggaatt gatgtgcacg gttaaaacag tcgcataatt
     5461 ttgaacttca agaggcatcc atgattcatc cgaccgcgat tgtccatccg aacgcgaagc
     5521 tcgccgccga tgtcgaggtg ggcgcctatt ccatcatcgg tgagcatgtc gagatcgccg
     5581 agggcacggt gatcggtccg catgtggtga tcaacggtca cacccgcatc gggcggcaca
     5641 accgcatttt tcagttctgc tcgctgggcg agatccctca ggacaagaaa tacgccaacg
     5701 agccgacgcg gctggagatc ggcgaccaca acaccatccg cgagttctgc accttcaatc
     5761 tcggtaccgc gcaggatgtc ggcgtgaccc gtgtcggcaa tcacaactgg atcatggcct
     5821 atgtacatct ggctcatgac tgtcaggtcg gtaacaacac gatcttcgcg aacaactcgc
     5881 agctggcagg gcacgtacat gtcggcgatt acgcgatcct tggcggcttc accgtggtgc
     5941 atcagttcgt caggataggc gagcacatca tcaccggcat gggaaccatc ctgttcaagg
     6001 atgtaccacc ctatgtgctg gcttcgggca atccctgtgc accgcacggc attaattcgg
     6061 aaggcctgaa gcggcgcggt ttttccagcg aagcgatcat gtcgatcaag cgcgcctaca
     6121 agacgttgta taagtccggc ctgagcctgg atgaggcgaa agcggcgatc gatgcgcaat
     6181 tggcggagca cgccgagttg cagttgctgg ccgatttcct tgcgacctcc acccgcggca
     6241 tcgtgcgttg atctagaaag ccccctcatg cagcagaaaa aaatcgtaat cggcatcgtc
     6301 gcgggcgagg cgtcgggcga catgcttggc agtcatctga tggcggcgct taagcaagcg
     6361 cgcccggaca ttcatttcgt cggtatcggc gggccaaaga tgcagtcggt cgggatggaa
     6421 gtgttgttcc cgatggagaa gctcgcggtg cgcggctatg tcgaggtgtt gcgccactat
     6481 cgcgagatcg tcggtatccg ccgcaagctg cgcgcccatt tcctgaaaca gcgtccggat
     6541 cttttcatcg cggtggatgc gccggatttc aacctcgatc tggaactggt gctcaaggcc
     6601 aacggcattc ccacgatcca ctatgtcagc ccgtcgatct gggcctggcg caaggagcgc
     6661 atccacaaga tcaagcgcgc cgtatcgcat atgctcgcgc tgtttccgca tgaacccagg
     6721 ctctaccagc aggccggtat cccggtagat tatgtcggtc atccgctggc tgacatgctg
     6781 ccggattatc cgaagcgcaa cgagatgcgt gagaccatgc gtattccact gcaggcccgg
     6841 gtattcgctt ttcttcccgg cagccgccag agcgagctga agcaactggc gcgcacctat
     6901 atcgagacgg ccaagctgat gttgcaacag tttcccgacg cgcgctttct ggtgccgctg
     6961 gtcagccgcg agacgcgcgg aatcttcgag caggcgctct atgattgcga tgcggagcaa
     7021 ttgcccatca cgctgctgtt cggtcatgca caggatgcga tgatcgccgc cgatggcgtg
     7081 ctggttgctt caggtacggc cacgctggag tgcgccttgc tcaagcgccc gatggtcatc
     7141 acctacaaga tgcctgcgct gacctggtgg ctgatgaagc gcaagagcta tcaaccctac
     7201 tatggcctgc ccaacatcct gtgcgaacgt ttcgtcgtgc ccgagttgat tcaggaagac
     7261 gcgacaccgg aaaacctggc tcaggcgctg ctcaatctgg tcaacgacaa ggatgtcgtg
     7321 gcaaagatcg aagaaacttt cctcgatctg caccggacct tgcgccagaa caccgcgcaa
     7381 aaggcggcgg cggccattct tccctatctt ccggcatgag tgccatcatc ctggtctgcg
     7441 gtgtagacga ggctggacgc ggccccctgg ccgggccggt gagtgctgcg gcagtgatac
     7501 tcgatcctgc ctatcccatc gaaggtttgg ccgactccaa gaaactttcc gaacgcaaac
     7561 gcgacttgct tgcgcctgtc attcgcgaac gcgcgctggc ctgggcggtg gcttatgccg
     7621 aagtggaaga gatcgacagt ctgaatatcc tgcaggccac gctgctggcg atgcgcaggg
     7681 ccgtgctggc gctgccgatc cagccgcagc atgtgctggt ggatggcctg tattgcccgc
     7741 aaaccggcat ccccagcgag gccatcgtca agggcgacag caaggtcgcc gcgatctctg
     7801 ctgcctcgat tctggcgaag accgcgcgcg acgagttgat gctgaagatg catgagcagt
     7861 tcccgcacta cggtttcgcc gagcacaagg gctatccgac ggccgcgcac ctcgccgcat
     7921 tgcgcgaaca cggtgtgtca gcggtgcacc gcaagagttt caagccggtg cgtgagttgt
     7981 tgcagtacac tcgccaaaac aacgagtgag gaaatcatga gcttgttcaa actgatccat
     8041 ctgcttgctg tcctggtctg ggtcggcggc atgttctttg cctatgtggt gctacgtccg
     8101 gcggccgtcg aggtgcttca gccaccggag cgcctgcgcc tgtgggacag cctgtttcgc
     8161 cgctttttta actgggtgtg gctggccatt ggtgtgctgc tggtgagcgg tttctacatg
     8221 gtttacctgt acggcggttt ggcctctgtg ccgcgtcatg tgcacatcat gctggcactg
     8281 gggctggtga tgatggcggt ctatggttat gtgttctttg cctgttacgt gccgctcagt
     8341 ctgcatgtcg ccaagcagcg ctgggcggag gcgggcgaac aactggggcg gatacgcaaa
     8401 ctggtcgcgt tcaatcttac gctcgggttg ttgaccgtga gtgtggcggt aatcggggtg
     8461 gcgctgtcct gacatcatgg aaacggcgct ccttgccgga gtcgtaacaa cggtcaatca
     8521 ctgcatgaac tgcggtgcct gctgcacaaa ttttcgggtc agcttctatt gggccgagtc
     8581 tgacgatgcg ttgggcggcc tcgttccggc gcacctgacc gagaatatct ctccacattt
     8641 gcgctgcatg aagggtacga ctggtaagcc ggtgcgctgt attgcgctcg aaggcgaagt
     8701 cggagtatcg gtttcgtgcg gcatctaccc gctccgctcc tctacttgcc gagagttcga
     8761 tgtgttcgag gcggacggca gcatcaatgc gcgctgcaat tccctgcgta tcggattagg
     8821 actagacccg atagacctct ccctatagtg ctgaagaagt ttcgttgtta aattggcgaa
     8881 cgtaattctg gcgggctaca ccgggttacg agagatactc tgaattcatc ttatggaggt
     8941 gtgcagtgga caatatcgga acaagcgcaa aacaggtctt cgccttcgag gaaggtgatg
     9001 gcaagaacaa gaagctgttg ggcggcaagg gtgccaatct ctgcgagatg actcagatcg
     9061 ggctgaacgt gccgccagga ttcgtcatca gtaccgaagc ctgtctgagc tatctcgctg
     9121 cctccggcga ggtgttgccg aagtcgctgc tggatgagat caatgcccag atgaaggcgc
     9181 tggaggccaa gaccggcaag cgtttcggcg atgcggcggc acccttgctg gtctcggtgc
     9241 gttcgggctc ggcgatgtcc atgcccggca tgatggacac catactcaat cttggtctca
     9301 atccaaagac gctggcgggc gagatcgccc agaccggcga tccgcgcttc ggctatgaca
     9361 cctaccgccg ttttattcaa ctgttcggca agatcgcgct ggatatctcg gacgagttgt
     9421 tcgacgcgga gctgaatgcg gtcaaacagg atcgtggcat taaggaggat ctggcgctga
     9481 ccgccgatga cctcaaggaa atcagcgagc gattcctgcg cgtggtgcag gagcagaccg
     9541 gcagggcatt tccggaagat ccctatgagc agctcatcct ttcggtcaaa gcggtgttcc
     9601 gctcctggat ggggaaacgc gcggtcgatt atcgtcgcga gttccatatc accaaggaaa
     9661 tggcgaacgg cacggcggtg aacatctgca cgatggtatt tggcaatcgc ggcaacgatt
     9721 cggccaccgg cgtcggcttc acgcgcaatc cggccaccgg cgagaatcac atcttcggcg
     9781 agtatctggt caatgcgcag ggtgaggacg tggtggcagg catacggacg ccgaagccga
     9841 tcgactatat ggcgaccgag atgcccgatc tgttccggca attgcaggag ctgcgcgcca
     9901 agctggaaag tcactatcac gaggtacagg atttcgaatt cacgatcgag cgcggcacgc
     9961 tttattgtct gcagacgcgc aacggcaaga tgaataccac cgcgatggtg cgcacctcgg
    10021 tagagatgga gaaagaaggc ctgatcacgc gccgtcaggc attgctgcgg attgcgccgg
    10081 atagtctggc gcagatgctg ttcccgcgtc tcgacccggc ttccaaagtc accccgctgg
    10141 ccaaagggtt gcctgcctct cccggagccg attatggtca tgcggtgttc gatgcggatc
    10201 gggccgagga acagggcagg gcaggcaagc cggtgatcct ggtgcgcgag gaaaccaaac
    10261 cggaagacat tcatggtttc ttcgcttcgc gcggcattct gaccagtcgc ggcggcaaga
    10321 cctcgcatgc ggccgtggtc gcgcgcggca tgggcaagcc ctgcgtggcc ggtgccgagg
    10381 gcatcgaggt caac
//
LOCUS       DASUIV010000089         5900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1136428, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000089 DASUIV010000000
VERSION     DASUIV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136428"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFW59_03340"
     CDS             <1..409
                     /locus_tag="VFW59_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5363279.1"
                     /translation="KLSDDEFAIMKTHPALGGEVLATAESSLPAPSRFLHIGRDIATG
                     HHEKWDGSGYPAGLKGEAIPVSARLMAVADVYDALISHRVYKKAFSHEEAVAVIAEGR
                     GTHFDPDAIDAFLAIQDVFRQIAGAYSDAPENA"
     gene            570..1946
                     /locus_tag="VFW59_03345"
     CDS             570..1946
                     /locus_tag="VFW59_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5363280.1"
                     /translation="MKNPDTFFDNLCFRISESNLAVRAEQLRARLKLYPTIMLSQMLI
                     EPSYVWLIWRTDAVPHLHLLLWLACAYLVHLAQFALWYSRGNELDSIAQCNAWSRRSF
                     LFSLCLGMMWGVGTLFFLPPDLMTQMVLICMMLGLVAGATATDSTHPPAFYAFVLGLM
                     LPLLFRVLVEQDSVHFALGFLMLLFLLVVLVSGHFLDKRVLVTLWQHFENQALARQLA
                     ALHDHLEQKVEERTAQLRHKTEEVEHIRDVTIIALGTLAETRDNETGNHLKRTQHYIR
                     ALALKLRDHPSFRNFLTGDNIEALHKLAPLHDIGKVGIPDRILLKPGKLTPEEFEIMK
                     RHPALGGDVLAVAERSLPAPSRFLYIARDIATGHHEKWDGSGYPSGLKGEAIPISARL
                     MAVADVYDALISRRVYKEPFSHADAVATIAQGRGTHFDPDAIDAFLAIQEEFRAIAEQ
                     YCDEAAVA"
     gene            complement(2080..3351)
                     /gene="cqsA"
                     /locus_tag="VFW59_03350"
     CDS             complement(2080..3351)
                     /gene="cqsA"
                     /locus_tag="VFW59_03350"
                     /inference="COORDINATES: protein motif:HMM:NF005526.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxyketone-type quorum-sensing
                     autoinducer synthase"
                     /protein_id="HEX5363281.1"
                     /translation="MKNNDNQQIPCANEPEFLKRRVNRYYRDRVMNTWNGGHIMRGRM
                     PRQDSILLSSNDYLSLAKHPQILHATAEHLLNEGGGLLMSGIFLHGTSPLLKLEKKLS
                     QFMCAGAGILCQSGWCANTGLIQSIADEQTPVYIDMLAHMSLWEGIRSAGATAIMFHH
                     NDPEHLERQLLKHGPGVIVVDSVYSTNGSICPLRDIVELGATHGCVLVVDESHSLGTH
                     GENGEGLVVSLGLTDRVHFRTVSLAKAFAGRAGFIACSERFSDYFKFESNPAIFSSTL
                     LPHEVAGLDATLTVIKSEGWRRKKLHANADYLRQRLDELGYNLNGSDSQIISLESGTE
                     QQTIVLRDALEARGIFGSIFCAPATPKNRALMRFSTHANLTTSELDRIVQVCADIRDE
                     IGLENWSSSRRKRMNSMSAATTDKEREAVPS"
     gene            complement(3590..3889)
                     /locus_tag="VFW59_03355"
     CDS             complement(3590..3889)
                     /locus_tag="VFW59_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363282.1"
                     /translation="MTDHSSEFHADNAKAARTLKAVVRLAKHLKIDLSDESLRNAFIA
                     QAAIDLKAAKRRHLSLPAWERITALYPIRPGITPRYTQIALNTYQEIDALPMSPE"
     gene            complement(3886..4242)
                     /locus_tag="VFW59_03360"
     CDS             complement(3886..4242)
                     /locus_tag="VFW59_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363283.1"
                     /translation="MGGGTGGSPSQSGASIAARSAMGAASSPADVTVSISGASRAALA
                     ADNLKVASAKTVQPTANSEPDQTNEIEMDRQNGAPITVDAPNSIAAGKQKLLHEGEWD
                     IDDLLLALLLLRVHRT"
     gene            4362..4610
                     /locus_tag="VFW59_03365"
     CDS             4362..4610
                     /locus_tag="VFW59_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363284.1"
                     /translation="MSEPYGWFFIRGSPFVYKFVFRVMRGASRRVNRCDQYGYPGSYA
                     ATRERQTALEEDALLAQSEYGCGAARGMMRALSVMPIR"
     gene            4622..>5900
                     /gene="alaS"
                     /locus_tag="VFW59_03370"
     CDS             4622..>5900
                     /gene="alaS"
                     /locus_tag="VFW59_03370"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX5363285.1"
                     /translation="MKSSEIRQKFLDFFASKGHTVVPSSSLVPHEDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIKYAWELLTEVYKLPKDKLTVTVYAEDDEAYDIWANEIGVPTDRIIRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGEHIWGGPPGSPEEDGDRFIEIWNNVFMQFNRD
                     EQGVMHPLPKPSVDTGMGLERISAVLQGVHANYEIDLFQNLICAAARVTNTSDLSNNS
                     LKVLADHIRACSFLIADGVIPGNEGRGYVLRRIIRRAIRHGYKLGARAAFFHKMVPDL
                     VAEMGAAYPELAAAQVRVMEILKQEEERFFATIEHGMAILENELIDLKGILGKIDFEA
                     GTPPSSKQIRPLSGEVAFKLHDTYGFPLDLTQDV"
BASE COUNT         1148 a         1704 c         1777 g         1271 t
ORIGIN      
        1 caagttgtcc gacgatgagt tcgccatcat gaagacccac cccgcgctgg gcggcgaggt
       61 gctggccacc gccgaaagca gcctgcccgc acccagccgt ttcctgcaca tcgggcgcga
      121 catcgccacc gggcatcacg agaagtggga cggcagcggt tatccggcgg ggctcaaggg
      181 cgaggccatc ccggtttccg cgcggctcat ggcggtggcc gacgtgtacg acgccctcat
      241 cagccatcgc gtgtacaaaa aagcgttcag ccacgaagag gcggtggcgg tcatcgcgga
      301 ggggcgcggc acgcatttcg atcccgacgc gatcgatgct tttctggcga tacaggatgt
      361 gttccggcag atcgccggag cgtatagcga cgcgcctgag aacgcatgac tattgatgta
      421 tggcacgacc ctctcccggc cttcggccac cctctcccgc aagcgggaga ggggatgttg
      481 gcgctcccct cgcccgcttg cgggagaggg gctgggggag agggagcaaa ccaatcgctg
      541 cgcgagtttt acgatgcatt tttgcgacta tgaaaaatcc ggataccttc ttcgataatc
      601 tgtgcttccg catcagcgaa agcaatctgg ccgtgcgcgc agagcagttg cgcgcacggc
      661 tgaaactgta tccgaccatc atgctcagcc agatgctgat cgagccgtct tacgtctggc
      721 tgatctggcg caccgatgcc gtgccgcacc tgcacctgct gctgtggctg gcgtgcgcct
      781 acctggtgca tctggcgcaa ttcgcgctgt ggtactcgcg cggcaacgag ctcgacagca
      841 tcgcgcagtg caacgcgtgg agccgccgtt cattcttgtt ctcgctgtgc ctgggcatga
      901 tgtggggcgt cggcacgctg tttttcctgc ccccggatct gatgacccag atggtgctga
      961 tatgcatgat gctggggctg gtcgcgggcg ctaccgcgac cgactcgacg cacccccccg
     1021 cgttctacgc ttttgtgctg ggcctgatgt tgccgctgct ttttcgcgtg ctggtcgagc
     1081 aggatagcgt gcattttgcg ctgggtttcc tgatgctgct gttcctgctg gtggtgctgg
     1141 tttccggaca ttttctggat aagcgcgtgc tggtgacgtt gtggcagcac ttcgagaacc
     1201 aggcgctggc gcggcaactt gccgcgttgc acgaccatct cgagcagaaa gtggaagagc
     1261 gcaccgcgca attgcgccac aagaccgagg aggtcgagca tatccgcgat gtgaccatca
     1321 tcgcgctggg caccctagcg gagacgcgcg acaacgagac gggcaaccac ctcaaacgca
     1381 cccagcacta cattcgcgcg ctcgccctca agctgcgcga ccacccgagc ttcaggaact
     1441 ttctcaccgg ggacaacatt gaggcgctgc acaaactcgc tcctttgcac gacatcggca
     1501 aggtcggcat cccggatcgc atcctgctca aaccgggcaa gctgaccccg gaagaattcg
     1561 aaatcatgaa gcggcatcct gcgctgggcg gcgatgtgct ggcggtagcc gaaagaagcc
     1621 tgcccgcgcc gagccgcttt ttgtacatcg cgcgcgacat cgccaccgga caccacgaga
     1681 aatgggacgg cagcggttat ccgtcaggac tcaagggcga ggccattccc atttccgctc
     1741 ggctcatggc ggtggccgac gtgtacgacg cgctcatcag tcgccgcgtg tacaaggagc
     1801 cgttcagcca tgcagacgcg gtagcgacca tcgcgcaagg gcgcggcacg catttcgatc
     1861 ccgatgcgat cgatgccttt ctggcgatac aggaggagtt ccgcgcaatc gcggagcagt
     1921 attgcgacga ggccgctgta gcctgaggcg cgtttgttct ggcttggacg ggttgcagga
     1981 tgggacgaac gtcggtgtcg ggcattgcca tatgccggag ggagtgccgc cagcatatgg
     2041 cggtcaaccg tgtcgatgac cgactacggc aggtttcgat cagcttggca cggcttctct
     2101 ctccttatcg gtggttgcgg cagacattga gttcatgcgt ttgcgacgcg aggatgacca
     2161 gttttccaga ccgatttcat cgcggatgtc cgcgcaaacc tggacgattc gatcaagctc
     2221 cgaagtcgtt aaattcgcgt gagttgaaaa tcgcatcagc gctcgatttt tgggggtggc
     2281 aggagcgcaa aatatcgagc cgaaaattcc gcgcgcttca agcgcatccc gtaaaacaat
     2341 ggtttgctgc tcggtgccag actcaagaga aattatttgc gagtcgctgc cgttaaggtt
     2401 gtatcccagt tcgtcgagac gctggcgcaa atagtcggca ttggcatgca gcttttttcg
     2461 ccgccagcct tccgatttga tgacagtcaa ggtcgcatcg agtccggcta cttcatgcgg
     2521 cagcaaggtc gagctgaaaa tggccgggtt agactcgaat ttgaaatagt cactgaagcg
     2581 ttcggagcag gcgatgaatc cggctcttcc tgcaaacgct ttcgcgagac tgacagtgcg
     2641 gaaatgcacg cggtcagtca agcccaacga tacgaccagc ccttcgccat tttcgccatg
     2701 tgtgcctagg gagtgggact catctacgac cagcacgcag ccgtgcgtag cgcctaactc
     2761 cacgatgtcc cgcaacgggc agatgctgcc gttcgtgctg tacacggaat cgaccacgat
     2821 cacgccgggg ccgtgcttga gcaattgcct ctccagatgc tccgggtcgt tgtggtggaa
     2881 catgattgct gtggcgccag cgcttcgaat cccttcccat aaggacatat gcgcgagcat
     2941 gtcgatgtac acgggcgttt gctcgtccgc gatggattga atcagaccgg tgttggcgca
     3001 ccatcccgac tggcacagta tgccagcgcc tgcgcacatg aactgcgata gttttttctc
     3061 cagttttagc agcgggctag tcccatgaag aaaaatgcct gacattaata gtcctcctcc
     3121 ctcattcagc aaatgttcgg ccgtggcatg caaaatttgg gggtgtttag ctaacgataa
     3181 gtaatcattg ctgctcaata aaattgaatc ctgccgaggc attctgccgc gcataatgtg
     3241 gccgccattc catgtgttca ttactctgtc cctgtaatag cggttcacac gccgtttgag
     3301 aaactcaggc tcgtttgcac acggtatttg ttggttgtca ttgtttttca cgactgtctc
     3361 ctattggttg taagtaagtt ttggcgtata gccccgcact gcggcgctat accggcgaca
     3421 gtctaaggag ttgctattaa ttcttgtttt tgttctgcgg caggggcggt agcgcgaagg
     3481 atgcgcggcg gggcaaaagg ttggcaggcg ggcattggcg caggtgcgcc ggctgcggcg
     3541 ctgaagaacg atgcgctcgc gcgcgactgc tggcgcgcat ggcaatgagt tattccggac
     3601 tcatcgggag ggcatctatt tcctggtagg tgttcagggc gatttgcgta taccggggcg
     3661 taattcctgg ccttatgggg taaagcgccg tgatcctttc ccacgcgggc aatgacaggt
     3721 ggcggcgctt tgcagctttc aggtcgattg cggcttgcgc gataaaggca ttgcgcaggc
     3781 tttcgtcgct caagtcgatc ttcaagtgtt ttgcgagtcg cacgacggcc ttcagggtgc
     3841 gcgcagcctt tgcattgtcg gcatgaaatt cactggagtg atctgtcatg ttctatgcac
     3901 tcgcagcaac aatagcgcca acagcaagtc atcgatatcc cactctccct cgtgcaaaag
     3961 tttttgtttg cctgcggcaa tactgttggg ggcatcgaca gtgatgggcg cgccattttg
     4021 ccggtccatt tcaatctcat tcgtctgatc cggctcactg ttggcggtcg gctggacagt
     4081 ttttgcggat gccactttca ggttgtcggc agccagcgca gccctggatg cgccggaaat
     4141 gctgaccgtg acatccgcag gcgaggaggc cgcacccatc gcgcttcgcg ctgcgatact
     4201 tgcgccgctt tgcgaggggg agccgcccgt tccaccgccc atgccaactc cgcttacacc
     4261 tccgaccgat ccgtccatga tgtatcgtcc ttttaagggt ttgaatgcct gtgtggctgg
     4321 tcttgccatt tggccgatgg gggcatcatc gtgaaatggg catgtctgaa ccatacggtt
     4381 ggttcttcat tcgcggctcg ccgtttgtgt acaaattcgt ttttcgggtg atgcgcggcg
     4441 cttcgcggcg ggtgaatcgc tgcgaccagt acgggtatcc cggcagctac gctgcgacac
     4501 gggaacgcca gacggcgctc gaggaggatg cgcttttggc tcagtccgaa tatggctgcg
     4561 gagccgcgcg tggtatgatg cgcgccttgt ccgtgatgcc aattcgataa tcaggtgcgc
     4621 aatgaaaagt agtgagattc gtcagaaatt tctggatttt ttcgcctcca agggccatac
     4681 cgtcgttcct tccagttcgc tggtcccgca tgaagacccg acgttgttgt ttaccaacgc
     4741 ggggatgaac cagttcaagg acgtgttcct cggcttcgac aagcgcccct atacccgcgc
     4801 cactacttcg cagaagtgcg tacgcgcggg cggcaagcac aacgatctgg agaacgtcgg
     4861 ctacaccgcg cgccaccaca ctttcttcga aatgctgggc aacttcagtt tcggcgacta
     4921 cttcaagcgc gacgcaatca agtatgcgtg ggagttgctg accgaggtct acaagctgcc
     4981 caaagacaag ctcaccgtga ccgtgtatgc cgaggacgac gaggcttacg acatctgggc
     5041 gaacgagatc ggcgtgccga ccgaccgcat catccgcatc ggcgacaaca agggcgcgcg
     5101 ctacgcttcc gacaacttct ggatgatggg cgacaccggc ccgtgcggcc cctgcaccga
     5161 gattttctac gaccacggcg agcacatctg gggcggccca cccggctcgc ccgaagaaga
     5221 cggcgaccgc ttcatcgaaa tctggaacaa cgtgttcatg cagttcaacc gcgacgagca
     5281 gggcgtgatg catccgctgc ccaagccatc ggtggacacc ggcatggggc tggagcgcat
     5341 ctcggcggtg ttgcagggcg tgcatgccaa ctatgagatc gatctgttcc agaacctgat
     5401 ctgtgcggcg gcgcgggtca ccaacaccag cgacctcagc aacaactcgc tgaaggtgct
     5461 ggccgaccac atccgcgcct gctcgttcct gatcgccgac ggcgtgatcc ccggcaacga
     5521 agggcgcggc tacgtgctgc gccgcatcat ccgccgcgcg atccgccacg gctacaagct
     5581 gggcgcacgc gcagcgttct tccacaagat ggtgcccgac ctggtcgccg agatgggtgc
     5641 cgcctatccc gaactggccg ccgcgcaagt gcgcgtgatg gagatactca agcaggaaga
     5701 ggagcgcttc tttgcgacca tcgaacacgg catggcgata ctggaaaacg agttgattga
     5761 tttgaagggg atactgggta agattgattt tgaagctggt actccacctt cctcaaagca
     5821 gataaggccg ttaagtggtg aagtggcatt caagctgcac gacacctacg gtttcccgct
     5881 cgacctgacg caggacgtgt
//
LOCUS       DASUIV010000090        10005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_300484, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000090 DASUIV010000000
VERSION     DASUIV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300484"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..636
                     /locus_tag="VFW59_03375"
     CDS             145..636
                     /locus_tag="VFW59_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363286.1"
                     /translation="MSTDYVFSSPKVMESIQCAECIKVWRLSNGRLIQKESEKPHIEA
                     STALREARKAVLECIRRIAQCYCEQQSFPTKKSEHKHLTTIGRFRKGYATYLKWRKEG
                     RAMHKILMYDEPTSQGEALKWAKSVASSLGLFSELTEVLDQLSQREIALSHAERQIVY
                     RAL"
     gene            716..1012
                     /locus_tag="VFW59_03380"
     CDS             716..1012
                     /locus_tag="VFW59_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363287.1"
                     /translation="MKLKSKLLLLVVSTIFSACTTYQQSGLTGGYKEKEIEPRVYDLY
                     YYFNGPSALFGNNLENWHKRAAELCGGKESYDVIEGPEQILFPQAKHGKIKCKS"
     gene            complement(1208..1565)
                     /gene="rnpB"
                     /locus_tag="VFW59_03385"
     ncRNA           complement(1208..1565)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFW59_03385"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1648..2718
                     /locus_tag="VFW59_03390"
     CDS             1648..2718
                     /locus_tag="VFW59_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5363288.1"
                     /translation="MKEPFITETRARLRTWLALGYALFIAYASLSPFTGWRELGLNFR
                     DVLEAPLSLTYTAFDAIVNLLSYLPLGLLLGLTLRARFGAVASVLLGLVAGALLSASM
                     EYLQMYLPTRISSNMDLLSNSAGALLGALLAVSLTSWTQLFALLVNWRSRLFHHGHEM
                     DFGLALLVLWMFGQINPSLPMLGNVFISEVARQPFAELPPAPFDLRESAAVTLNLLML
                     GTLLMTLLRAPRNVVSALLLVLSAVALAKFVTAALLLKSWALLLWINSEAVLGILSGM
                     LLLSALLWPPRAVAIRLGAAVASGYFVIVNFLLNDNTPAAAANLYHWHYGHLLNYNGL
                     AQTISLTFPLLLLIHLFRLRRA"
     gene            2802..3113
                     /locus_tag="VFW59_03395"
     CDS             2802..3113
                     /locus_tag="VFW59_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5363289.1"
                     /translation="MSFFDKHVFFCLNQRDDGSDCCNNHGAQKAREYVKDKVKELGIS
                     NRHNNIRINSAGCLGRCDEGPVVVVYPEGTWYTYVDESDLDDIIEEHLKNGRVVERLK
                     I"
     gene            3113..3724
                     /locus_tag="VFW59_03400"
     CDS             3113..3724
                     /locus_tag="VFW59_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5363290.1"
                     /translation="MASQRKLVFPGPAGSLEGMLHLPDGAPQAVAVIAHPLPIMGGTM
                     DNKVVTTLTRTFVELGYATLRFNFRGVGESAGSYDEGRGETVDAIAAAQFMRDEFGEL
                     PLITAGFSFGGYVQARAALELKPQRMVLVAPAVGRFEMPPVPADTLLIHGDMDEVVAL
                     DDLLSWARPQHLSVVVLTGAEHYFHGRLTQLKQIVAQQLRGLA"
     gene            3739..4653
                     /locus_tag="VFW59_03405"
     CDS             3739..4653
                     /locus_tag="VFW59_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5363291.1"
                     /translation="MTTVLSAQALCKSYGGQIVVNGLNLTIRRGECFGLLGPNGAGKT
                     TTLRLLLGLIAPDSGEVKLLGHAVPQHAREARLRVGVVPQMDNLDPDFTVAENLMVYG
                     RYFGMKDREIEARLPDLLEFANLSHKRDAKVPTLSGGMKRRLTVARALVNDPDVIFLD
                     EPTTGLDPQARHLIWQRLRELTQQGKTLILTTHFMDEAERLCHRLAVMDNGRLISEGS
                     PRELIANNIEPQVIEVFGEHAASWAQEHAARHAQRFEVSGESVFCYVDDARPLLLELQ
                     QQPQLRYLHRPANLEDVFLKLTGREMRE"
     gene            4650..5432
                     /locus_tag="VFW59_03410"
     CDS             4650..5432
                     /locus_tag="VFW59_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5363292.1"
                     /translation="MRTNHFSLPRLSLRFVPIWRRHFMVWKKMAGPSILGHLADPVIY
                     MLGLGYGLGSMLPEVGGMPYIAFLAAGTVCYSTMNAASFEAMYSGFARMHEQRTWDAI
                     LNTPITLDDIVLSEIVWGASKSLLSGVAVLAVIWLLGLSHSPLTLWLIPLSLLIGLCF
                     AALGLIITAIAPGFEFFMYYFTLVITPMMLLCGVFFPVEQLPAALQAVSSALPLTHAI
                     DLARPLMKDTIPANWLLHAAVLLGYAMTGFYVSLALFRRRLL"
     gene            5570..5980
                     /locus_tag="VFW59_03415"
     CDS             5570..5980
                     /locus_tag="VFW59_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5363293.1"
                     /translation="MLWIKAFHIIFVVSWFAGLFYLPRLFVNHAMATEPAEIARLKLM
                     EGKLYRFVTPIGALAIAFGFWLWFGYGFSGGWLHAKTALVAFLAGYHLYCGHLVKEFS
                     ADRNTRSHVFYRFFNEVPVLILVAVVILVTVKPF"
     gene            5984..7126
                     /locus_tag="VFW59_03420"
     CDS             5984..7126
                     /locus_tag="VFW59_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX5363294.1"
                     /translation="MPDFFAPCPRGLETLLRSDLETMGAQNLRATDGGVQFTGDWPLC
                     YRVNLESRLASRVLWRVADTQYRSEQDVYKAVFDLQWQRWFDVTNTIRVNTTAIRCPL
                     KSLEFITLLVKDAICDRFRAHCNERPSVDTLEPDVRIHVFIEDDKLMLYLDTSGDALF
                     KRGVRQYTNIAPLRENLAAGILRMTGWQPGTPLVDPMCGSGTFLIEAAQMSLNIQPGI
                     ARRFAFEKLKNFDATTWNRMREQAIAAQLPPAPLELYGSDLYGDALKTASRNLQEAGL
                     AECVELKQANVLEITAPAEHGILIANLPYGERMGELDELAELYPQLGDALKKKFGGWT
                     AYLFTADKAILKLMRLSPSKRTPLFNGAIECRLLEYKIVAGSNRPK"
     gene            complement(7149..8165)
                     /locus_tag="VFW59_03425"
     CDS             complement(7149..8165)
                     /locus_tag="VFW59_03425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5363295.1"
                     /translation="MPDNEAARLDALKAYGMLDTPPESFYDDIVQLAAYICQTPIAAI
                     SFIDGERQWFKASVGVAVSEIPRQDAFCAHAILQPDDIMIVKDAFHDPRFSNNPLVTG
                     NTPVHFYAGVPIVTDAGEALGTLCVIDTKPRDLGEPVLSALRKLARQIMAHLELGKSL
                     AELQNCQTQLSQVNQRLAEQNITDDLTQLKNRRGFQLALNAEWERTFRYSNPLSLLLI
                     DVDECKAYKDKFGRAACDEALLKVAQLIGHSARELDSIARLGDDEFAVVLPETDSEGA
                     FQIAERIRYRIELADWPNQPVTVSIGVTSYNGQADADTLLVDADQALRRAKQIGRNCT
                     MSTI"
     gene            complement(8186..9322)
                     /locus_tag="VFW59_03430"
     CDS             complement(8186..9322)
                     /locus_tag="VFW59_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5363296.1"
                     /translation="MERDQATELIHNLLRGMISKKASDLFVTAGFPPAFKVDGKMTPV
                     SNQALTAVHTQELARAIMNDRQAAEFEATHECNFAISPSGIGRFRVNIFMQQQRVGMV
                     LRTITTKIPNLDEMGLPEVLKDIVMTKRGLVILVGGTGSGKSTTLAAMIGHRNHNSYG
                     HIITIEDPVEYVHEHANCIITHREVGVDTENWHNALKNTLRQAPDVILIGEIRDRETM
                     EYAVAFAETGHLCMATLHANSANQALDRIINFFPEERREQLLMDLSLNIKALISQRLI
                     PKKDGVGRAAAIEILLNSPLIADLIFKGEVHAIKEVMAKSRELGMETFDGALFNLYES
                     GTIGYEEALKNADSVNDLRLRIKLQSKLSGDRDVMSGTDHLKLT"
     gene            complement(9426..>10005)
                     /locus_tag="VFW59_03435"
     CDS             complement(9426..>10005)
                     /locus_tag="VFW59_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5363297.1"
                     /translation="LTVEDPIEFVHESKKCLINQREVGPHTLSFQNALRSALREDPDV
                     ILVGEMRDLETIRLAMSAAETGHLVFGTLHTSSAAKTIDRIIDVFPADEKEMVRAMLS
                     ESLRAVISQALLKTKDGSGRVAAHEIMICTPAIRNLIREAKVPQMYSAIQTGQNMGMQ
                     TLDQCLTSLIKANVITPAEARSKAANKDLFPG"
BASE COUNT         1988 a         3068 c         2894 g         2055 t
ORIGIN      
        1 gctcctttca gtcgcaggat gctgcgcgat aaagtcgcgc agcgccggtt agttctacat
       61 taggcccttc gtatgagcag aactgagacc gactcgtttt cgtataagtg cccctgtgga
      121 gttggtacga tagaaaaaac tattgtgtcg accgattatg tattttcttc gccgaaagtg
      181 atggaaagca ttcaatgcgc ggagtgcata aaggtgtggc ggctttccaa tggccgctta
      241 atccagaaag aaagtgaaaa acctcatatt gaggcgagca cggcgcttcg tgaagcgcgc
      301 aaagcagtct tggaatgcat ccgtcgaatc gcacagtgtt attgcgaaca gcaatccttc
      361 cccacgaaaa agtctgaaca taagcaccta acgactattg gtcgctttag gaaaggttac
      421 gcaacgtatc tcaaatggag aaaagaaggc agagcgatgc ataaaattct tatgtatgac
      481 gagcctacct cgcaaggcga agcattgaaa tgggctaaat ccgttgcaag ctctttgggt
      541 ctcttcagcg aactaacaga agttcttgac caactctcgc aacgagaaat cgcattgagt
      601 cacgcagaga gacaaatcgt atacagagcc ttatgaatat cgtgcagtgg cccaacccgg
      661 cagccccttg cttttacatt agcaatcgta aataacaacc gcaggaataa ccacaatgaa
      721 attgaaatca aagttattgt tactagtcgt ttccacgatt ttttcagcat gtacgacgta
      781 ccagcaatcg ggactcacag ggggctacaa ggaaaaggaa atagaaccta gagtctatga
      841 tctttactat tacttcaacg ggccatcagc attatttgga aacaatcttg aaaactggca
      901 taagcgggca gcagagctat gcggaggaaa agaaagttac gatgtaattg agggacctga
      961 gcaaatatta ttccctcagg ccaagcatgg aaagatcaag tgtaaatcat aggtcattaa
     1021 ttaaaaaaac aaatggggac ggagcccgac atttagttac cactacaact aacggggccc
     1081 gcatcacatt taaaaggcat agcgggctgt cgcccgtcat gccttaatgc ttttggattt
     1141 cgctttcccc tgtgcgccgc cgcaggacga cccgaattaa agggtgcgct agtgtgaatc
     1201 tgaaatgagg tgaagctggc cggtaagccg ggttctgtcg tggacagtca ttcctctagg
     1261 cgtttcgtta ccttacgctc aagcaaccta cccgcaagcg acgcgagcca cgtcatagcc
     1321 tgcctatttg gttttgctcc ggatggggtt taccctgcca ctgatgttgc caccagcgcg
     1381 gtgagctctt acctcgccat ttcaacctta cctgatcccg acaaaagtcg ggccatcggc
     1441 ggtgtatttt ctgtggcact ttccatcgcc tcacggcgtc cggccattaa ccggcatccc
     1501 gctctgcgga gcccggactt tcctccccgc accctaagat gcgcggcgac tgtctagcca
     1561 gcttcggcgc ggagtctatc acgagcgcac tgtcatcccc ttttccatgt tgacacccct
     1621 cgcgatttcg ccaacactcc gctgcccatg aaagaaccct tcatcaccga aacccgcgcg
     1681 cgactgcgca cctggctggc gctgggttat gcgctattca tcgcctatgc gagcctgtcg
     1741 ccgttcaccg gctggcgcga gctggggctg aatttccgcg acgtgctcga agcgccgctg
     1801 tcgctgacct ataccgcgtt cgatgcgatc gtcaatctgc tgtcctatct gccgctcggg
     1861 ctgttgctcg gcctgacgct gcgcgcacgc ttcggcgcgg ttgccagcgt gctgctcgga
     1921 ctggttgcgg gtgcgctgct gtcggcgagc atggaatatc tgcagatgta cttgccgacg
     1981 cgcatcagct cgaacatgga tctgctgtcc aatagcgcag gcgcgctgct gggtgcgctg
     2041 ctggcggtga gcctcacctc gtggacgcaa ctgttcgcgc tgctggtgaa ctggcgcagc
     2101 cgcctgttcc atcatggcca tgagatggat ttcgggctgg cgttgctggt gctgtggatg
     2161 ttcggccaga tcaacccctc gctgccgatg ctgggcaatg tgttcatcag cgaagtcgcg
     2221 cgccagcctt tcgccgaact gccgcccgcg cccttcgacc tcagggaaag cgccgccgtc
     2281 acgctgaatc tgctgatgct gggcacgctg ctgatgacgc tgctgcgcgc gccccgcaac
     2341 gtggtgtcgg ccctgctgct ggtgctgagc gccgtcgcac tggccaagtt cgtcaccgcc
     2401 gcgctgctgc tcaaatcctg ggcgctgctg ctgtggatca acagcgaggc ggtgctgggc
     2461 atcctgtccg gcatgctgct gctgtccgcg ctgctgtggc cgccgcgcgc ggtggccatc
     2521 cgtctcggcg cagcggtcgc ctcgggctat ttcgtgatcg tgaacttcct gctgaacgac
     2581 aacacgccgg ctgccgccgc caatctctat cactggcact acggccacct gctcaactac
     2641 aacggactgg cgcagaccat ctcgctgacc ttcccgctgc tgctgctgat ccacctgttc
     2701 cgcctccgca gggcatagcg cggcggacgc tgctcgatcc ggctcgccgc cgccggcccg
     2761 gactctttat aatcccgtcc cattcaaccc cggagcattc catgagtttt ttcgacaagc
     2821 atgttttctt ctgcctcaac cagcgcgacg acggcagcga ctgctgcaac aaccacggcg
     2881 cacagaaggc gcgcgaatac gtcaaggaca aggtcaagga actgggcatc tccaatcgcc
     2941 acaacaatat ccgcatcaac tccgccggct gtctggggcg ctgcgacgaa ggcccggtgg
     3001 tagtggtcta tccagaaggc acctggtaca cctatgtgga cgagagcgac ctggacgaca
     3061 tcatcgaaga acacctgaag aacggccgcg tggtcgaacg cctgaagatc tgatggcatc
     3121 gcaacgcaag ctcgttttcc ccggcccggc gggatcgctc gaaggcatgc tgcacctgcc
     3181 cgacggcgcg ccgcaggccg tcgccgtgat cgcgcatccg ctgcccatca tgggcggcac
     3241 catggacaac aaggtggtca ccacgctgac gcgcaccttc gtcgagctgg gctatgccac
     3301 gctacgcttc aattttcgcg gcgtgggcga gagcgcaggc agttacgacg agggacgcgg
     3361 cgagaccgtg gatgccatcg cggccgcgca attcatgcgc gacgagttcg gcgagctgcc
     3421 gctcatcacc gcaggattct ccttcggcgg ctacgtgcag gcccgcgcgg cactggaact
     3481 gaagccgcag cgcatggtgc tggtcgcccc cgccgttggc cgcttcgaga tgccgccggt
     3541 gcctgccgac acgctgctga tccacggcga catggacgag gtggtggcgc tggacgacct
     3601 gctgtcatgg gcgcgtccgc agcatctgtc cgtcgtggtg ctgaccgggg cggagcacta
     3661 cttccacggc cgcctgaccc agctcaagca gatcgtcgcg caacagctgc ggggactcgc
     3721 ctgatgcaag agggcgacat gaccacggta ctgagcgcgc aagcgctgtg caaatcctac
     3781 ggcggccaga tcgtggtgaa cgggctgaac ctgaccatcc ggcgcggcga atgcttcggc
     3841 ctgctcggcc cgaacggcgc gggcaagacc accacgctgc gcctgctgct cggcctgatt
     3901 gcaccggatt cgggcgaggt caagctgctc ggccatgcgg tgccgcaaca cgcccgcgag
     3961 gcgcggctgc gcgtcggcgt ggtgccgcag atggacaatc tcgatccgga tttcaccgtg
     4021 gcggaaaacc tgatggtgta tggccgctac ttcggcatga aggaccgcga gatcgaggcg
     4081 cgcctgcccg acctgctgga gttcgccaac ctcagccaca agcgcgacgc caaggtgccg
     4141 acgctgtccg gcggcatgaa gcggcgcctg accgtggcac gcgcactggt caacgacccg
     4201 gatgtgatct tcctcgacga acccaccacc ggcctcgacc cgcaagcgcg ccacctgatc
     4261 tggcagcgac tgcgcgaact gacgcagcaa ggcaagacac tgattctcac cacccacttc
     4321 atggacgaag ccgagcggct gtgccaccgc ctcgcggtga tggacaacgg ccgcctcatc
     4381 agcgaaggct cgccgcgcga actgatcgcg aacaacatcg aaccgcaggt gatcgaggtg
     4441 ttcggcgaac acgcggcaag ctgggcacag gaacatgcgg cacgccatgc gcaacgcttc
     4501 gaggtcagcg gcgagagcgt gttctgctac gtggacgatg cccggccgct gctgctggaa
     4561 ttgcagcaac agccgcaact gcgctacctg caccgccccg cgaacctcga agacgtgttc
     4621 ctgaaactga ccggacggga gatgcgcgaa tgagaacgaa tcatttttcc ctgccgcgtc
     4681 tgagtctgcg cttcgtcccg atctggcgac gccatttcat ggtgtggaag aagatggccg
     4741 gaccatccat cctcggccat ctcgccgacc cggtgatcta catgctgggg ctgggttacg
     4801 gtctgggctc gatgctgccg gaagtcggcg gcatgcccta catcgccttc ctcgccgccg
     4861 gaacggtgtg ttacagcacc atgaacgccg ccagcttcga ggcgatgtat tccggcttcg
     4921 cccgcatgca cgagcagcgc acctgggatg cgatcctgaa cacgccgatc acgctggacg
     4981 acatcgtgct ctccgaaatc gtctggggcg cgagcaagag cctgctgtcc ggggtggcgg
     5041 tgctggcggt gatctggctg ctgggcctgt cgcactcgcc gctgacgcta tggctgatac
     5101 cgctgtcgct gctgatcggc ctgtgcttcg ccgcactggg gctgatcatc acggcgatcg
     5161 cgcccggctt cgagttcttc atgtactact tcacgctggt gatcaccccg atgatgctat
     5221 tatgcggcgt gttttttccg gtcgagcagc tgcctgcggc gctgcaagcc gtatcgtccg
     5281 cgctgccact gacgcacgcc atcgatctgg cccggccgct gatgaaggac acgatacccg
     5341 ccaactggct gctgcatgcc gccgtactgc tggggtacgc aatgacgggt ttctatgtgt
     5401 cgctggcgtt gttcagaaga agactattgt aggagcggct tcagccgcga aaagcagcgt
     5461 cgataaatgg cggcgccatc gcggctgaag ccgctcctac gatgaacgca caggattttt
     5521 atcgatattt ttcgcttccg cgaaaacttc caagttgcag gagtgtttca tgttgtggat
     5581 caaggcattt catatcattt tcgtggtgag ctggttcgcc ggcctgttct atctgccgcg
     5641 cctcttcgtc aaccacgcga tggcgaccga gcccgccgag atcgcgcggc tgaagctgat
     5701 ggaaggcaag ctgtaccgtt tcgtcacgcc catcggcgcg ctggcgatcg ccttcggttt
     5761 ctggctgtgg ttcggttacg gcttctccgg cggctggctg catgccaaga ccgcactggt
     5821 cgcctttctg gccggctacc acctgtattg cggccatctg gtgaaggaat tttccgcaga
     5881 ccgcaacacc cgcagccatg tgttctaccg cttcttcaac gaggtgccgg tgctgatcct
     5941 ggtcgccgtc gttattctcg tcacagtcaa acccttttaa gccatgccag actttttcgc
     6001 cccctgcccg cgcggcctcg aaacgctgtt acgcagcgac cttgaaacca tgggcgcaca
     6061 gaacctgcgc gccaccgacg gcggtgtgca gttcaccggc gactggccgc tgtgctatcg
     6121 cgtcaatctg gaaagccgcc tcgccagccg cgtgctgtgg cgcgttgcgg atacgcaata
     6181 tcgcagcgaa caggatgtct acaaggccgt gttcgatctg cagtggcagc gctggttcga
     6241 tgtcaccaac accatccgcg tcaacaccac cgcgatccgc tgcccgctga agagcctgga
     6301 gttcatcacg ctgctggtca aggatgcgat ttgcgaccgc ttccgcgcgc actgcaacga
     6361 gcgccccagc gtggatacgc tggagcccga cgtacgcatc cacgtgttca tcgaagacga
     6421 caaattgatg ctgtatctgg acacctccgg cgatgcgctg ttcaagcgcg gcgtgcgcca
     6481 gtacaccaac atcgcaccgc tgcgcgagaa cctcgcggca ggcattctgc gcatgaccgg
     6541 ctggcaacca ggcaccccgc tggtcgatcc gatgtgcggc agcggcacct ttctgatcga
     6601 agcggcgcag atgtcgctga acatccagcc cggcatcgcg cgccgcttcg cgttcgagaa
     6661 gctgaagaat ttcgatgcga cgacatggaa caggatgcgc gaacaggcta tcgccgcgca
     6721 actcccgccc gcaccgctgg agctctacgg cagcgacctg tacggcgatg cgctgaagac
     6781 cgcctcgcgc aacctgcagg aagccggact ggccgaatgc gtggaactca aacaggccaa
     6841 cgtgctggag atcaccgccc cggcggaaca cggcatcctg atcgccaacc tgccctacgg
     6901 cgaacgcatg ggcgaactgg acgaactggc cgagctgtat ccgcaactgg gcgatgcgct
     6961 gaagaagaaa ttcggcggct ggacggctta tctgttcacc gccgacaagg ccatcctgaa
     7021 gctgatgcgc ctgtcgcctt cgaagcgcac cccgctgttc aacggtgcga tcgaatgccg
     7081 tttgctggaa tacaagatcg tggcgggcag caaccgtccg aagtagggcc gcaggcaaac
     7141 atctccgttc agatcgtact catggtgcag ttgcgcccga tctgcttggc tcggcgcaag
     7201 gcctggtcag catcgaccag cagtgtatcg gcatcggcct gtccattgta actggtcacg
     7261 ccgatactga cggtcacagg ctgattgggc cagtcggcca attcgatgcg gtaacggatt
     7321 ctttcggcga tttgaaatgc gccctcgctg tcagtttcag gcaatacaac cgcaaactca
     7381 tcatcgccaa gacgagcaat cgaatccagt tcgcgcgcgc tatggccaat caattgcgca
     7441 accttcagca acgcctcgtc gcacgcggcc cggccaaact tgtctttata agccttgcat
     7501 tcgtcgacat cgattagcag caggctgagc ggattcgagt agcggaaagt acgctcccat
     7561 tcggcgttca gagccagctg gaagccacgg cgattcttga gctgggtgag atcgtcggta
     7621 atattttgtt cggcgagcct ctggttgacc tggcttaact gcgtctggca attctgcaat
     7681 tcggcgagcg acttgccgag ctcaagatgc gccatgatct ggcgtgccaa tttgcgcaat
     7741 gccgacaaaa caggctcacc cagatctcgc ggtttggtat cgatgacgca cagcgtacct
     7801 aaggcctcgc cggcatcagt cacgatgggc actcccgcat aaaaatgaac gggggtattg
     7861 ccggtcacca gcggattgtt cgaaaagcgc ggatcatgaa aagcatcttt cacgatcatg
     7921 atgtcatccg gttgcaagat cgcatgggca caaaaagcgt cttgcctggg aatttcgctg
     7981 actgccacgc ccacgctcgc cttaaaccac tgccgctctc cgtcgataaa ggaaatcgcg
     8041 gcaatcggcg tctggcaaat gtaagccgcc aattggacaa tatcgtcata gaacgactcg
     8101 ggcggcgtat cgagcatccc gtacgccttg agcgcatcca gtcttgcagc ttcgttgtct
     8161 ggcatcgagg cttgcatgtt ctcgatcagg tcagcttcag gtgatcggtg ccgctcatca
     8221 cgtcgcggtc gccgctcaac ttgctctgca gcttgatacg cagacgcaga tcgttgaccg
     8281 agtcggcatt cttcagcgcc tcttcgtagc cgatcgtgcc cgactcgtac agattgaaca
     8341 gcgccccgtc gaaagtctcc atgccgagtt cgcgcgactt ggccatgacc tccttgatgg
     8401 catgcacctc gcccttgaag atcaggtcgg caatcagcgg cgaattcagc aggatttcga
     8461 tggccgccgc acggccgacg ccgtccttct tcggaatcag gcgctgcgag atcagcgcct
     8521 tgatgttcag cgacaggtcc atcagcaact gttcgcgacg ctcttccggg aagaagttga
     8581 tgatgcggtc cagcgcctga ttggcgctgt tggcgtgcag cgtggccatg cacaggtggc
     8641 cggtttcggc gaaggccacg gcgtattcca tggtctcgcg gtcgcggatc tcgccgatca
     8701 gaatcacgtc cggcgcctga cgcagcgtgt tcttcagcgc attgtgccag ttctcggtgt
     8761 ccacgccgac ctcgcggtgg gtgatgatgc agttggcatg ctcatgcacg tattcgaccg
     8821 gatcctcgat ggtgatgatg tgaccgtagc tgttgtggtt acggtgtccg atcatcgccg
     8881 ccagcgtggt ggatttaccg gaaccggtgc cgccgaccag aatcaccaac ccgcgcttgg
     8941 tcatcacgat gtctttcaac acctccggca aacccatctc atccagattc gggatcttgg
     9001 tggtgatggt gcgcagcacc atgccgacgc gctgctgctg catgaagatg ttcacacgga
     9061 agcggccgat gcccgatgga ctgatcgcga agttgcattc atgcgtcgcc tcgaactcgg
     9121 cagcctgacg gtcgttcatg atcgcacgag ccaactcctg cgtatgcacg gcggtcagcg
     9181 cctgatttga aaccggcgtc atcttgccgt ccaccttgaa tgcgggcgga aagcctgcgg
     9241 tgacgaacaa gtcggaggct tttttgctga tcatcccgcg tagcaggtta tggatcagct
     9301 cggtggcctg gtctctttcc attgaatact cctaaatccg ggtgagccgg aatgtaaaac
     9361 ggtggaatgc aggaaggcac gaaaaacttt tgttcgggca cccacacggc atgcccctac
     9421 ggcgatcaac ccgggaacag atccttgttc gcagccttgc tgcgagcctc tgcgggagtg
     9481 atgacattag ccttgatcag actggtcaga cactgatcca gcgtctgcat gcccatgttc
     9541 tgtcctgtct ggatcgcgga atacatctgc ggcaccttgg cttcgcggat caggttacgg
     9601 atcgcagggg tacagatcat gatctcgtgc gccgccaccc ggcccgaacc gtccttggtc
     9661 ttgagcagcg cctgcgagat caccgcacgc agcgactcgg acagcatcgc acgcaccatc
     9721 tccttctcgt cggcagggaa cacgtcgatg atacggtcga tggtcttggc cgccgaactg
     9781 gtgtgcagcg tgccgaacac cagatggccg gtctcggctg cagacatcgc cagacggatg
     9841 gtctccagat cgcgcatctc gcccaccaga atcacgtccg gatcttcacg caacgcgctg
     9901 cgcagcgcgt tctggaacga cagcgtatgc gggccgactt cacgctggtt gatcaaacat
     9961 ttcttgcttt catgcacgaa ttcgatcgga tcctcgaccg tcaag
//
LOCUS       DASUIV010000091         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_578853, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000091 DASUIV010000000
VERSION     DASUIV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578853"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1551)
                     /locus_tag="VFW59_03440"
     CDS             complement(229..1551)
                     /locus_tag="VFW59_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX5363298.1"
                     /translation="MKIKPYILAAMLGSSGLVQAADLLETFHAAQANDPVFAAARASK
                     DAGMEKLPQGRSLLLPSVNLNANSTFNSQTVRYLGPTPFPAGTQRYNSHGYSVNLVQP
                     LFRQQNWMAYTESELQVAQTEAQFKIAEQDLILRVAQAYFDVLIAQDSVQLTEAQKTA
                     ISEQLEQAKRNFEVGSATITDTHEAQARFDLTTAQEIAAQNDLEIKRRSLQQLIDTMP
                     KDLAHLGKGFKLEAPQPADMEKWVADAELNNPQLAIAQAGAEIAEKEVARNRGGHYPT
                     VDLVAGYSKTNSNGGNFGVGSDVSGKSVGVQLNMPLFAGGAVNSKWREAEANRERAKQ
                     ELENARRTVATQTRQAYLGVVSGIARVKALQQALTSSESVLEASKLGQEVGVRTNLDV
                     LNAQQQVYATRRDLYQAEYTYLASHLRLKAAVGALGEEELNKVNLALH"
     gene            complement(1629..2285)
                     /locus_tag="VFW59_03445"
     CDS             complement(1629..2285)
                     /locus_tag="VFW59_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX5363299.1"
                     /translation="MQNVEQARFNMIEQQIRPCEVLEGRILELLKHVRREQFVPAAMK
                     ELSFVDMEIPLGYGASMWSPKLEARVVQELHLSRKDKVLEIGSGSGYLTALLSSLAGQ
                     VTSVEIVPELSAMAKQNLAAFGRNNVTFEVGDAAQGWGNGASYDVIVLTGSTPVLPEA
                     FKNSLNVGGRLFAIVGDAPVMEAKLITRVAPDTFESVNIMEACVASLQNAAQPERFVF
                     "
     regulatory      2401..2494
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2522..>2916
                     /locus_tag="VFW59_03450"
     CDS             2522..>2916
                     /locus_tag="VFW59_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235537.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX5363300.1"
                     /translation="MNANSPFLAETAHVDEAAVKPLPNSRKIYVEGSRPDIRVPMREI
                     SQTDTQINNGIEKNPPIYVYDCSGPYTDPSARIDIRSGLTELRAPWIAERDDTEALDQ
                     LTSTYGQQRLNDPALAGMRFNLKHTPRRA"
BASE COUNT          580 a          927 c          794 g          615 t
ORIGIN      
        1 caattagtga acggcaggca ttacaacagc cccggacatg ctctgtcaag ggcgcctcaa
       61 aacatcaatc attcccgaaa gaaggagcgt agcgaagctt cgaagcacgg ccacatggga
      121 gttcagcttg ttaatttaaa cagggctccc gcgaaggccg gagcgacgaa accgaggttt
      181 ttcggaggcc tcgggcgtga gatgaacgca cccccactgc aatgaggatt agtgcaaggc
      241 gagattaacc ttgttaagct cttcctcgcc cagtgcgccc actgcggctt tcaggcgcag
      301 gtggctagcg aggtaggtgt attcggcctg atagaggtcg cggcgggtgg catagacctg
      361 ttgctgtgcg ttcagcacgt cgagattagt acgcacaccg acttcctgtc cgagtttgct
      421 ggcttccagc acgctctcgc tggaggtgag tgcctgctgc agagccttga ctcgggcgat
      481 gccgctgacg acgccgagat aggcctgacg agtctgggtc gccacggtgc ggcgtgcgtt
      541 ctcgagttct tgcttggctc gttcgcgatt cgcctcggcc tcgcgccact tggaattcac
      601 cgcgccgcct gcgaacaacg gcatgttcag ctgcacgccc acgctcttgc cgctgacatc
      661 gctccccacg ccgaaattgc cgccgtttga attggtcttg gagtagcccg ccaccagatc
      721 gaccgtgggg tagtggccgc cgcgattgcg cgccacttcc ttctcggcaa tctcggcgcc
      781 cgcctgcgcg atggcgagct ggggattgtt cagctcggca tcagctaccc atttttccat
      841 gtcggctggc tgcggcgcct ccagcttgaa tcccttgccc aggtgcgcca ggtctttcgg
      901 catcgtatcg atcagctgct gcaggctgcg gcgcttgatt tccagatcgt tctgcgccgc
      961 gatctcctgc gccgttgtca gatcgaaacg tgcctgcgcc tcgtgggtgt cagtgatggt
     1021 ggcactgccc acctcgaaat tgcgcttggc ctgctccagt tgctcggaga tagccgtttt
     1081 ttgcgcctcg gtaagctgta cgctgtcctg cgcaatcagc acgtcgaaat aggcctgtgc
     1141 cacacgcagg atgagatcct gctcagcgat cttgaattgc gcctcggttt gagcaacctg
     1201 caactcggac tcggtatagg ccatccagtt ttgctggcgg aacagcggct gcaccagatt
     1261 cacgctgtag ccatgggagt tgtagcgctg tgtacctgcc ggaaacggtg tcggcccgag
     1321 ataacggacg gtctggctgt tgaacgtgct gttcgcattg agattgacgc taggcaacag
     1381 cagcgaacgc ccctgcggca gcttttccat ccccgcatct ttggacgcgc gcgcggcagc
     1441 aaacaccgga tcgttcgcct gtgcggcatg gaaagtttcc agcagatcag cggcctgaac
     1501 caggccgctg ctgccgagca ttgctgccag tatgtagggt ttaattttca tcggtttcgc
     1561 gccaatctta tatattagca aactcttatg ctacagcctc gagccgcttc gcgcaatccc
     1621 gatgaggctc agaatacgaa ccgctcgggc tgagccgcgt tctgcagcga cgcaacacag
     1681 gcctccatga tattgaccga ctcgaaggtg tccggtgcga cacgggtgat cagcttggct
     1741 tccatcaccg gcgcatcgcc gacgatagcg aacaatcggc cgccgacatt caaactattc
     1801 ttgaacgcct ccggcaacac cggcgtagat ccggtcaaca cgatcacgtc atagcttgcg
     1861 ccattacccc aaccttgcgc cgcatcgccc acttcgaacg ttacattgtt gcggccgaat
     1921 gcagcgagat tctgcttggc catggcactc agctccggca cgatctcgac cgaagtgacc
     1981 tgccccgcca acgaagacaa cagcgcggtc agatatccgc taccggaacc gatctccagc
     2041 accttgtctt tacgggacag atgcaactcc tgcaccacgc gcgcctccag cttcggcgac
     2101 cacatgctgg cgccataacc cagcggaatc tccatatcga caaaagacaa ttccttcatg
     2161 gccgccggca cgaactgttc gcggcgcaca tgcttgagca attccagaat ccggccttcg
     2221 agcacctcgc aagggcgaat ctgctgctcg atcatgttga agcgcgcctg ttccacgttc
     2281 tgcatctcct tactccattc cctgttttat tgtttcaact gtcaatttgc cgacgaagta
     2341 taacaacaca ccccggctcg gcatagtaac ttgcttttcc cccgcctttc cagtaccttg
     2401 cggcctcgct aggggtctcc gcgatgacgg agtgagacac accctttgaa cctgtacggg
     2461 tcatgccgtc gtagggaagc atccacccgg tgctttctga tattttttgg agcaccaccg
     2521 aatgaacgcc aattcccctt ttttagccga aaccgcgcat gtcgacgagg ctgcggtcaa
     2581 gccgctgccg aactcccgca agatctatgt cgagggctcc cgcccggaca tccgcgtgcc
     2641 gatgcgcgag atcagccaga ccgacacgca gatcaataac ggcatcgaaa agaaccctcc
     2701 tatctatgtc tacgactgct ccggcccgta caccgacccg agcgcaagaa tcgacatccg
     2761 ctccggcctg accgagctgc gcgcgccgtg gatcgccgaa cgcgacgaca ccgaagcgct
     2821 ggatcaactg acttccacat acggccagca gcgcctgaac gatccggcac tggccgggat
     2881 gcgcttcaac ctgaaacata ccccgcgccg cgcaaa
//
LOCUS       DASUIV010000092         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_858087, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000092 DASUIV010000000
VERSION     DASUIV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858087"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFW59_03455"
     CDS             complement(<1..582)
                     /locus_tag="VFW59_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEX5363301.1"
                     /translation="MFKNMKIGARLGLGFGLVLVLLSMIAFVGITRLGGLNNDVDNMA
                     NDKYPKTVLANNLIDAMNTVARVNRNTLLTDDPAELDKQRAIRDEARKQIGEIMQKLE
                     DTVKSEAGIKKVEAIKEARTAYVTVLDKFIETNKSGDRKGAIAMLFGEFSPVTAAYLK
                     SINDLIDFQDELMKKSGEEANQSYLEARNMMITM"
     gene            complement(804..>2513)
                     /locus_tag="VFW59_03460"
     CDS             complement(804..>2513)
                     /locus_tag="VFW59_03460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX5363302.1"
                     /translation="TDNWHLSLRFGPNVIREGMDPLAFLRYLATLGRIVHIVTLTDAV
                     PPLSASDPEACYLGFEVSFESEADKATIEGVFEFVREDSEIHILPPRSKIGEYLNLIS
                     KMDGDNLRLGDMLVSCGTLTDAELDMALRIQAETGKATHPIGTVLVEQHLVQGAVVEA
                     ALEKQKQIKDGKSGEGKGGEVSLIRVDSEKLDQLINLVGELIIAGAGTTLIAQRAGMP
                     ELVEATATLSRMVEEVRDSALSLRMVQIGGTFTRFQRVVRDVSKDLGKDIRLEIAGAE
                     TELDKTVVEKIGDPLTHLVRNSMDHGIEPAEVRLARGKPAHGTLKLNAYHDSGSIVIE
                     VSDDGGGLNKDKILAKAIERGLVTPGQPMSDREIYNLIFEAGFSTADTVSNLSGRGVG
                     MDVVRRNIQALRGTIDIESEMGRGSTMRIRLPLTLAIIDGFLVGVSDAAYVVPLDMVV
                     ECIELPTGQAAEGDNHYLNLRGEVLPYLRLRDLFEVEGQAGRRENVVVVQYAGHKAGI
                     VVDSLLGEYQTVIKPLGKVFGGARGFGGFTIMGSGEVALILDVPRLMQQVSNRETAAG
                     QVS"
BASE COUNT          429 a          828 c          704 g          552 t
ORIGIN      
        1 catggtgatc atcatgttgc gcgcttccag ataggactga ttcgcctctt cgccggattt
       61 tttcatcagc tcgtcctgaa aatcgatcag gtcgttaatc gatttcaaat aggccgccgt
      121 caccggagaa aattcgccga acagcatcgc gatcgcgccc ttgcggtcac cgctcttgtt
      181 ggtctcgata aacttgtcca gaaccgtcac ataggcggta cgtgcttcct tgatggcctc
      241 gaccttcttg atccccgctt cggatttgac ggtgtcttcg agcttctgca tgatctcgcc
      301 gatctgcttg cgcgcctcat cgcggatggc ccgctgcttg tcgagttcgg cgggatcgtc
      361 tgtcagcagg gtattgcggt tgacccgcgc cacggtattc atcgcatcga tcagattgtt
      421 ggccaaaacc gtcttgggat atttgtcgtt ggccatgttg tcgacatcgt tgttaaggcc
      481 gcctaacctc gtgatgccga cgaaggcgat catcgacaat aacacgagca ccaggccgaa
      541 acccaagccc agacgagcac cgattttcat attcttaaac atgatttcac ttccttttca
      601 tcaaaataaa tacaacatgt gattacccac caccgattcg ccgcgttcct gccagtcata
      661 ctcagaatcg ttcacccacc ggatatccac cacagcccga tcagcatgat caacagcggc
      721 ggcatggcca gcacccattc gttaaatgac tttttcatta ccttcctctt cgcggctcct
      781 accgctgcta taaaacaccg cccctacgac acctgtcccg ctgcggtctc gcggttgctc
      841 acctgctgca tcagtcttgg cacgtccagg atcagcgcca cttcgccgct gcccatgatg
      901 gtgaagccgc cgaagccgcg cgcgccgccg aacaccttgc ccagcggttt gatcacggtc
      961 tggtattcgc ccagcaggct gtccaccacg atgcccgcct tgtgtcccgc gtattgcacc
     1021 accaccacgt tttcgcggcg cccggcctgc ccttccactt cgaacaggtc gcgcagcctc
     1081 agatacggca gcacttcgcc gcgcaggttg aggtagtggt tgtcgccttc ggccgcttgg
     1141 ccggtcggca gttcgatgca ttcgaccacc atgtccagcg gcaccacgta ggccgcgtcg
     1201 ctcaccccga ccaggaagcc gtcgatgatc gccagcgtca gcggcaggcg gatgcgcatg
     1261 gtgctgccgc gacccatctc ggattcgatg tcgatggtgc cccgcagcgc ctggatgttc
     1321 cggcgcacca cgtccatgcc cacgccgcgc ccggacaggt tgctcaccgt gtcggcggtg
     1381 gagaacccgg cctcgaagat caggttgtag atctcgcggt cgctcatcgg ctgacccggc
     1441 gtgaccagac cgcgctcgat ggccttggcc agtatcttgt ctttgttgag accgccgccg
     1501 tcgtcagaca cttcgatgac gatgctgccg gagtcgtggt aggcgttcag cttcagcgtg
     1561 ccgtgcgccg gcttgccgcg cgccagacgc acctcggcag gctcgatgcc gtgatccatc
     1621 gagttgcgca ccagatgggt cagcggatcg ccgatcttct ccaccacggt cttgtccagt
     1681 tcggtttccg ctccggcaat ttccagacgg atgtccttgc ccagatcctt gctcacgtcg
     1741 cgcaccacgc gctggaagcg cgtgaaggtg ccgccgatct gcaccatgcg caagctgagc
     1801 gcagaatccc gaacttcctc gaccatccgc gatagcgtgg cggtggcttc caccagttcc
     1861 ggcatgcccg cgcgctgggc gatcagggtg gtgcccgcac ccgcgatgat cagctcgccg
     1921 accaggttga tcagctggtc gagtttctcg gagtcgacgc ggatcaggct gacttcgccg
     1981 cccttgcctt cgccgctctt gccgtccttg atctgtttct gtttctccag cgcggcttcg
     2041 accaccgcgc cctgcaccag atgctgttcg accagcacgg tgccgatcgg atgcgtggcc
     2101 ttgccggtct cggcctgtat ccgcagggcc atgtccagct cggcatcggt cagcgtgccg
     2161 cagctcacca gcatgtcgcc cagacgcagg ttgtcgccat ccatcttgct gatgaggttc
     2221 aggtattcgc cgatcttgct gcgcggcggc aggatgtgga tctcgctgtc ttcgcgcacg
     2281 aattcgaaca cgccttcgat ggtcgccttg tccgcctcgg attcgaagga gacttcgaag
     2341 cccagatagc aggcttccgg atcgctagcg ctcagcggcg gcaccgcgtc ggtcagggtg
     2401 acgatgtgga cgatgcgtcc cagcgtggcc aggtaacgca ggaaggccag cgggtccatg
     2461 ccttcgcgga tgacgttggg gccgaagcgc aacgagaggt gccagttgtc cgt
//
LOCUS       DASUIV010000093         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1138599, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000093 DASUIV010000000
VERSION     DASUIV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138599"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFW59_03465"
     CDS             <1..218
                     /locus_tag="VFW59_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HEX5363303.1"
                     /translation="ILDIILRDEIGHVGIGNRWYGYLCEQRGLEPLAAYAQLAAEHQA
                     PLLRGPFNLEARRAAGFSEQELAALGK"
     gene            413..838
                     /gene="rimP"
                     /locus_tag="VFW59_03470"
     CDS             413..838
                     /gene="rimP"
                     /locus_tag="VFW59_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX5363304.1"
                     /translation="MDVLKLVETTVTGLGYELVDVERSGRGLLRVFIDKPEGISVDDC
                     QVVSNQLTRLFTVENIDYDRLEVSSPGLDRPLKKESDFLRFAGQKAQLKLRMPIAGRK
                     NFVGVIGVLENGILQLDVEGSQIEIDLANLDKARLVPTF"
     gene            901..2373
                     /gene="nusA"
                     /locus_tag="VFW59_03475"
     CDS             901..2373
                     /gene="nusA"
                     /locus_tag="VFW59_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX5363305.1"
                     /translation="MSREILLLVDALSREKNVDKDVVFEALESALASATKRRFDDEAD
                     VRVSIDRNTGEYSSFRCWEVMDDETFETPDLHIKLEEARKRDPQIQLGEFIEEPLENI
                     DFGRIGAQAAKQVIFQKIRDAEREQVLADFMERKEHLVSGTIKRLERGNAIIEFGKIE
                     ALLPREQMIPKENLRVGDRARAHLLRVDRGVRGPQVILSRTSNDLLIKLFELEVPEIE
                     EKLLEIVSAARDPGSRAKIAVVSHDPRIDPIGTCVGMRGSRVQGVTNELAGERVDIIL
                     WSEDPATYVINALAPAEVSSIVVDEDKHSMDVVVEEENLAQAIGRGGQNVRLASELTG
                     WELNIMTVEQSNEKHAEEFSKIRTVFMEKLDVDEEVADILVQEGFNTLEEVAYVPLEE
                     MMEIESFDEATVEELRSRARNSLLTAAIANEEKVEHGIEDLLKLDGMDEQTARALTVK
                     GIATQEQLADLDVDELVELSGMDSERANSLIMAARAPWFA"
     gene            2407..>3798
                     /locus_tag="VFW59_03480"
     CDS             2407..>3798
                     /locus_tag="VFW59_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5363306.1"
                     /translation="MNIAQFATELGLPVALLLEQLKAAGVSKEQDSDQVSEKDKAQLL
                     EHLRQSHGADMPAKKITLTRRETTEIKKADSTGRARTIQVEVRKRRVVAPVGETAAPV
                     SAAAPKVDHVAVLNEQELAAREQEARLQAELAERQAAELVAKRSRSKSKAGEVEAVAE
                     AVVTPVAEVEEIVPVVEAPVVAAKPVEAAPTLVIGAPVARRVAKPVVKPVEKPVEKPV
                     AKPAEKAAATEGTLHKPTPKPGDKVERFTRPAKPGAKQSDKRDESGSHKRPAAKPDAG
                     MGGRPGAWSAPLRGGKRGGRHGKEAPAAAGESFMLPTEPIVREVSVPETITVAELALK
                     MSIKAAEIIKALMKMGSMVTINQVLDQETAMIVVEEMGHIAKAAKADDPDAYLLDSEV
                     AHDAVLESRAPVVTVMGHVDHGKTSLLDYIRRTRVASGEAGGITQHIGAYHVETERGM
                     ITFLDTPGHEAFTA"
BASE COUNT          804 a          971 c         1254 g          769 t
ORIGIN      
        1 ggattctgga tatcatcctg cgcgacgaga tcggtcatgt cggcatcggc aaccgctggt
       61 acgggtatct gtgcgagcag cgcggtctgg agccgctcgc ggcctatgcg caacttgccg
      121 ccgaacatca ggcgccgctg ctgcgcggcc cgttcaatct ggaagcccgg cgtgcggcgg
      181 gattcagcga gcaggaattg gcggcattgg gaaaatagcc ggttcaggca tgcgtccggg
      241 caaggcaaag gccgggtgcc cggcgagact attcgaaacc cgccgtttca tccccaaacc
      301 tgccattgcg gcccttccta acaatctgat tccatttgaa gcttggcttt gctataatgc
      361 gcggcgtttt gagatgggcc ttgagcccgt tttttatttg tgggtcgaaa ttatggatgt
      421 tttgaagctg gtcgaaacaa cggtaaccgg tctgggctat gagctggtgg atgtcgaacg
      481 ctctggccga ggcttgttgc gcgtcttcat cgataagccg gagggtatct cggtggatga
      541 ttgccaggtg gtgagcaatc agttgacgcg cctgtttacg gtcgagaata tcgactacga
      601 ccgtctggag gtgtcttcgc cggggctgga tcgtccgttg aagaaagagt ccgacttcct
      661 ccgctttgcg gggcagaagg cgcagctcaa gttgcgtatg ccgatagcag ggcgaaagaa
      721 tttcgtcggc gtgatcggcg tgttggaaaa tggaattttg cagctcgatg tagagggtag
      781 ccagattgaa atcgatctgg cgaatttgga taaggcccgt ttggtgccga cgttttagtc
      841 gtttggacgt ttgggttttg tgcctgtcgc cgacaggctg gtagatagcc ggagaatggt
      901 atgagtcgtg aaattttgct gctggtagat gcgttgtcgc gtgagaagaa cgtcgacaag
      961 gatgtcgtat tcgaggcgct ggaatctgct ttggcctcag cgaccaagag gcgcttcgat
     1021 gatgaggccg atgtgcgcgt gtcgattgat cgcaataccg gcgaatattc gtcgttccgt
     1081 tgctgggaag tgatggatga cgagaccttc gagacgcctg acttgcatat caagctggaa
     1141 gaggcgcgca aacgcgatcc gcaaatccag ctcggcgagt tcatcgaaga gcctctggaa
     1201 aacatcgact tcggtcgcat cggtgcgcag gctgcgaagc aggtgatctt ccagaagatc
     1261 cgcgatgccg agcgcgagca ggtgctggcg gatttcatgg agcgcaaaga gcacctggtc
     1321 tccggcacga tcaagcgtct ggagcgcggc aatgcgatca tcgagttcgg caagatcgag
     1381 gccttgctgc cgcgcgagca gatgatcccg aaagagaatc tgcgtgtcgg cgaccgtgcc
     1441 cgtgcgcatc tgttgcgtgt tgatcgcggt gtgcgcggcc ctcaggtgat cctgtcgcgt
     1501 acctccaacg atctgctgat caagctgttc gagctggaag tgccggagat cgaagagaag
     1561 ttgctggaga tcgtcagcgc agcccgcgat ccgggttccc gtgccaagat cgcggtcgtg
     1621 tcgcacgatc cccgcatcga tccgatcggt acctgtgtcg gcatgcgcgg ctctcgcgtt
     1681 cagggtgtga ccaacgagct tgcaggcgag cgcgtggata tcatcctgtg gtcggaagat
     1741 ccggctacct acgtcatcaa cgcgctggca cctgccgagg tgagcagcat cgtggtggat
     1801 gaagacaagc acagcatgga cgtggtggtc gaggaagaga atctggcgca ggccatcggt
     1861 cgcggcggtc agaacgtgcg tctggccagc gagctgaccg gctgggagct caacatcatg
     1921 acggttgagc agtccaacga gaagcatgca gaagagtttt ccaagattcg taccgtgttc
     1981 atggaaaagc tggatgtgga tgaggaagtg gccgacatcc tggtgcagga aggtttcaat
     2041 acgctggaag aagttgcgta tgtgccgctg gaagaaatga tggagatcga atcgttcgac
     2101 gaggcaactg tggaagagtt gcgcagccgt gcgcgcaatt ctctgctgac ggcggccatc
     2161 gccaacgaag agaaggtcga gcatggcatc gaggatttgc tcaagctgga tggtatggat
     2221 gaacaaacgg cgcgcgccct gacagtaaag gggatcgcga ctcaggaaca attggccgat
     2281 ttggatgtgg atgaactggt ggagctttcc ggtatggata gcgaacgtgc caattcgctg
     2341 attatggcgg cacgcgcacc ctggtttgca taaggcagcg gtatctagag gacggtaatg
     2401 ggaaaaatga acatagcgca atttgcgaca gagctgggat tgccagttgc attgctgctc
     2461 gagcagctga aggcggctgg agtaagcaaa gaacaggact ccgatcaggt ttctgaaaaa
     2521 gacaaggcgc aattgctgga gcatctgcgt cagtcgcatg gtgccgatat gcctgccaag
     2581 aagatcacct tgactcgtcg cgagacgacg gagatcaaga aggcggacag cacgggtcgc
     2641 gcacgcacca ttcaggtgga agttcgcaag cgccgtgtcg tggctcctgt tggcgagact
     2701 gctgctccgg tgagcgctgc cgcacccaag gtcgatcatg ttgcggtact gaacgagcag
     2761 gagctggctg cacgcgagca ggaagcccgc ctgcaggccg aactggccga acggcaagcc
     2821 gctgaattgg tggcaaagcg ttcacgcagc aagagcaagg ctggcgaagt cgaagcggtt
     2881 gccgaagctg ttgtgactcc cgttgccgag gtggaagaga ttgttcctgt ggtcgaggcg
     2941 cccgttgtcg cagcgaagcc cgttgaggct gcgccgactc tggtgatcgg cgcgccggtt
     3001 gcgcgccgtg tggcaaaacc cgttgtaaag ccggtcgaaa agcccgtcga gaaacccgtt
     3061 gcaaagcctg ctgaaaaagc tgctgcgacc gagggtacgc tgcacaagcc gacaccgaag
     3121 ccaggcgaca aggtcgaacg cttcactcgc ccggcgaagc cgggtgcaaa acaatccgac
     3181 aagcgcgacg aatccggttc gcacaagcgt ccggcagcca agcctgatgc aggcatgggc
     3241 ggtcgtcccg gcgcatggtc tgctccgttg cgcggcggca agcgcggtgg tcgccatggc
     3301 aaggaagcgc cggctgcggc aggtgaatcc ttcatgttgc cgaccgagcc tatcgtgcgt
     3361 gaggtttcgg ttcccgagac gattacggtt gccgaactgg ccctgaagat gtccatcaag
     3421 gctgccgaga ttatcaaggc gctgatgaag atgggttcga tggtgaccat caatcaggtt
     3481 ctcgatcagg agaccgcaat gatcgtggtc gaggagatgg gacacatcgc caaggccgcc
     3541 aaggccgatg atccggatgc ctatctgctg gacagcgaag tcgctcatga tgcggtgctg
     3601 gaatcgcgcg cccctgtcgt taccgtgatg ggccacgtcg atcatggcaa gacgtcactg
     3661 ctcgactata tccggcggac ccgcgtggct tccggcgagg ctggcggtat tacccagcac
     3721 atcggtgcct accacgtgga aaccgagcgt ggcatgatta ccttccttga cacgccgggt
     3781 cacgaggcct tcacggct
//
LOCUS       DASUIV010000094         9211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1232330, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000094 DASUIV010000000
VERSION     DASUIV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232330"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..1178)
                     /gene="pstB"
                     /locus_tag="VFW59_03485"
     CDS             complement(405..1178)
                     /gene="pstB"
                     /locus_tag="VFW59_03485"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX5363307.1"
                     /translation="MNTENITNPKVVVKDLNFYYGNYRALKDINLNIAEKMVTAFIGP
                     SGCGKSTLLRTFNRMYQLYPKQKATGDIILDGANILDPRQDLNMLRSKIGMVFQKPTP
                     FPMSIYDNIAFGVKLYHNLGKNEMDERVEWALKKAALWTEVKDKLKQSGTGLSGGQQQ
                     RLCIARAIAVKPQVLLLDEPTSALDPISTAHIEKLIDELKEDFTIVIVTHNMQQAARV
                     SDYTAYMYLGDLIEFGDTSTVFTNPKRKETEDYITGRFG"
     gene            complement(1201..2046)
                     /gene="pstA"
                     /locus_tag="VFW59_03490"
     CDS             complement(1201..2046)
                     /gene="pstA"
                     /locus_tag="VFW59_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX5363308.1"
                     /translation="MLNLYIRRRIVNATGLVVSMLAMAFGLFWLCWILWTLLVYGLPA
                     LGPIVFSQSTPPPGSTGGLLNAIAGTLMMTVLGTLIGTPIGILAGTYLAEFGHRGWLA
                     PITRFINDVLLSAPSIVIGLFVYEVYVIQVKHFSGWAGALALAIIVIPIVIRTTENML
                     RLVPNTLREAAAALGAPQWKVVNFVTLRAARAGIITGVMLAIARISGETAPLLFTALN
                     NQFWSSNMDQPMANLPVVIFQFAMSPYEDWQHLAWAGALLITISVLTLNILARVLFRQ
                     SATTH"
     gene            complement(2052..3017)
                     /gene="pstC"
                     /locus_tag="VFW59_03495"
     CDS             complement(2052..3017)
                     /gene="pstC"
                     /locus_tag="VFW59_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5363309.1"
                     /translation="MSISFLHGARLKRQVWLDMLFRGVTRLSAFAVLVLLAAIIGSLV
                     IGSMPAIHAFGFGFLTSAEWNPVTDEYGALVPIIGTLVTSGVALLIAIPVSFGIAIFL
                     TELSPRILRRPLGIAIELLAGIPSIIYGMWGLFIFAPLFADHIEPWINDNLGALPMLG
                     PFFTGPPMGISVLTAGIILAIMVIPFIASVMRDVFDVVPPLLKESAYGLGATTWEVVW
                     QVVLPYTRIGVVGGIMLGLGRALGETMAVTFVIGNAHDLSKSLLMPGNSISSALANEF
                     TEAVGELYTSSLIELGLILFFITFVVLSLARYMLYKLGKREGQAS"
     gene            complement(3113..4159)
                     /gene="pstS"
                     /locus_tag="VFW59_03500"
     CDS             complement(3113..4159)
                     /gene="pstS"
                     /locus_tag="VFW59_03500"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX5363310.1"
                     /translation="MKLKQLVIGITAATVMAYAGASLAAATITGAGATFPYPVYAKWA
                     EAYKAQTGVAMNYQSIGSGGGIKQIKAKTVDFGASDAPLNPNELGLAGLAQFPTVVGG
                     VVPVINVQGIAAGQLKLTGDTLSGIYQGKITKWNDPKIAADNKGINLPDQSITVVHRA
                     DGSGTSFIFTTYLSQVSTEWLRGVGAGSAVQWPVGTGGKGNEGVASYVQRIKGSIGYV
                     EYAYALQNKMNYAQLKNREGQFVAPTENNFKAAAANAKWDADKGFYEILTNQPGKESW
                     PITGATFILIHKSQDNAANAAEVLKFFDWAYGNGNKMAADLDYIPMPDNVTKMIRSAW
                     KAQIKDASGKAIWK"
     gene            complement(4281..5027)
                     /locus_tag="VFW59_03505"
     CDS             complement(4281..5027)
                     /locus_tag="VFW59_03505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX5363311.1"
                     /translation="MLELDRTVQSAAQPRLIAGLARNSDEILEAQRLRYQIFRDEMGA
                     NLPHSQKGLDRDIYDKYCEHLLVRESGDNNVVGTCRLLAPDQAQKIGGYYAQTQFDLT
                     RLLHLRGRMAEAGRTCIHRDYRDGATLNRLWDGIAQNLRQNRHEHLIGCVSIGIADGG
                     HVAASLYRKLHRIYGAPIEYRVFPRNPLPLEALNPYLDAPIPLLLKRYLRLGAYICGE
                     PAWDPAFNTADLLVLLPTSRLNSNANYLLK"
     gene            complement(5171..5998)
                     /locus_tag="VFW59_03510"
     CDS             complement(5171..5998)
                     /locus_tag="VFW59_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5363312.1"
                     /translation="MHNRPTLLSSLMRGALLMPHLAYGMALAIVYPHLTTGRRRRLLK
                     SWSKRLLSILHIGIQIEGTQPARSDGACLIVANHVSWLDSLVLNAIHPSRFITDLEVR
                     NWPLIGWLCQRGGSIFVERAMRPHANSVNRRIETLLQQGTCVTLFPEGPATDGRQVGH
                     FHSALIQPAIDAGIRLCPIALRYQDMDGQRSDHAAFIGDMTLPRSVWRMLRSPRFNAL
                     LVFTPALTTAGANRRVLARTAQEAIAQELKRTTTQRYLQPDTPAEQSSPLLTEPSIL"
     gene            complement(5991..7811)
                     /locus_tag="VFW59_03515"
     CDS             complement(5991..7811)
                     /locus_tag="VFW59_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5363313.1"
                     /translation="MKTLQGILERRALSALFQPIMDISNGLFLGFEGLIRGPADSPLH
                     SPLNLFAAAKQQDLALEVEMLSRQIVMEAFAAQRLPGKLFLNVSPDTLTHPSFKNGQT
                     LSYLEKIGIDPKQVIIEITENQPTFDFENMRNALLHYRSMGFQIAIDDLGAGFSSLRL
                     WSELRPEYVKVDMHFVQGINADPLKQQFLRSIQQIAQSSGTLVIAEGIETEAEFRTVC
                     DLGIDFGQGYFIARPTPTPSLVAPVEVSSQVNLANPPCHNANQIISSRQVTTEKLQRY
                     VEPVHPETLHENIFTRFTEDESLRAIPIVKGGIPVGLIDRQQYFSCFVHPFRRDLLGK
                     KPCSTCMGGAHCKDMSSHCINIEPLLVEKSTPIHELSDFLTETESSHFVSGFIITEQG
                     RYIGLGNAQDLLREITKMQIENARYANPLTLLPGNVPINEHIEHLLHSNQAFTVCYCD
                     IDHFKPFNDVYGYRKGDEAIQFTGRLLNWACDPRHDLLGHIGGDDFIMIMRSTDWEKR
                     CNQALSSFAQTSSVLFEEAHRAIGGYASEDRQGRVVHHPLPTLSIGVVCVTPGMFHSH
                     HEISEAATVAKKMAKKKPGNSLFVERRELNSNEVTEASYA"
     gene            7983..8219
                     /gene="rpmB"
                     /locus_tag="VFW59_03520"
     CDS             7983..8219
                     /gene="rpmB"
                     /locus_tag="VFW59_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX5363314.1"
                     /translation="MARVCQVTGKGPMTGNNVSHANNKTKRRFLPNLQRRRFWVETEN
                     RWVSLRLTNAALRTIDKNGIEAVLFEMRARGENV"
     gene            8231..8386
                     /gene="rpmG"
                     /locus_tag="VFW59_03525"
     CDS             8231..8386
                     /gene="rpmG"
                     /locus_tag="VFW59_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5363315.1"
                     /translation="MREKIKLESSAGTGHFYTTDKNKKTTPEKLEMKKFDPVVRKHVM
                     YKETKLK"
     gene            8524..>9211
                     /gene="dmeF"
                     /locus_tag="VFW59_03530"
     CDS             8524..>9211
                     /gene="dmeF"
                     /locus_tag="VFW59_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEX5363316.1"
                     /translation="MHTHDLSRWTHQHVFNQGSPAAERSTRAVMWITAAMMVVEIAAG
                     WWFNSMALLADGWHMSSHAFAIGLSAFAYAAARRYSTDPRFAFGTWKIEILGGFASAI
                     FLLGVAAMMIFGSVERLFSPQQIHYQEAIIVAVIGLVVNIVCAKILGHAHHGHDQHSH
                     AHADHDHHHHDLNLKSAYVHVIADAATSVLAIVALIGGWFYGWSWLDPVMGIVGAILV
                     AVWAKGLLIET"
BASE COUNT         1847 a         2396 c         2928 g         2040 t
ORIGIN      
        1 ggagcgtcgc tatgacgata cgcaacgccg ccgttacgat gaccgcagcg accggcagga
       61 gcgtcgttac gaggatcgtc gtgactgatc gttgattaaa aaaaaagccc ggtttttacc
      121 gggctttttt tgttgggggc aggacgatta accgaaatgc cttgcagtgt aatggctacg
      181 gcataaccta aaggttagtg gctccgacat aacatgctgc gcatgtgagt ctccgccgca
      241 acaagcctgc ggcttgttgt ctccgccgct gcgcggctta acattcgcca tgctcatgtt
      301 agcttccgcc gctacattac cggcaggtta accaaacctg ccatgtagtg gttccagtgt
      361 aacgcgctgc gcgcattagc tttcaccgtt acatcactgg acgattaacc gaatcgtcca
      421 gtgatgtagt cttcggtctc tttgcgtttc gggttggtga acacggtgct ggtgtcgccg
      481 aattcgatca ggtcgcccag atacatgtag gcggtgtagt cggagacgcg cgcggcctgt
      541 tgcatgttgt gggtcacgat gacgatggtg aagtcttcct tcagttcgtc gatcagcttt
      601 tcgatgtgcg cggtggagat cgggtcgagc gcggaggtcg gctcgtccag cagcagcacc
      661 tgcggcttga cggcgatcgc gcgggcgatg cacagacgct gctgctggcc gccggacagg
      721 ccggtgccgc tctgcttgag cttgtctttg acctcggtcc acaacgcagc cttcttcagc
      781 gcccattcga cgcgctcgtc catctcgttc ttgccgaggt tgtggtacag cttcacgccg
      841 aaggcgatgt tgtcgtagat cgacatcggg aacggggtgg gcttctggaa caccatgccg
      901 atcttggagc gcagcatgtt cagatcctga cgcggatcga ggatgttcgc cccgtccaga
      961 atgatgtcgc cagtcgcctt ctgcttgggg tagagctggt acatgcggtt gaaggtgcgc
     1021 aacagcgtgg acttgccgca gccggacggg ccgatgaagg cggtgaccat cttctcggcg
     1081 atgttcagat tgatgtcctt gagcgcgcgg tagttgccgt agtagaagtt cagatccttg
     1141 acgacgacct tcggattggt gatgttttca gtgttcattg ctagccttgt tgatagccaa
     1201 ttaatgggtg gtcgcactct ggcggaacag cacgcgtgcc agaatattca gtgtcagtac
     1261 gcttatggtg atcagcaacg cgcctgccca ggcgagatgt tgccagtctt cgtaggggct
     1321 catcgcgaac tggaagatca ccacgggcag gttggccatc ggctgatcca tgttgctgct
     1381 ccagaactgg ttgttcagcg cggtgaacag cagcggcgcg gtctcgccgc tgatgcgtgc
     1441 gatggccagc atcacgccgg tgatgatgcc cgcgcgggcc gcgcgcaggg tgacgaagtt
     1501 gactaccttc cattgcggtg caccgagcgc ggcagcggct tcacgcagcg tgttgggaac
     1561 cagacgcagc atgttttcgg tggtgcggat cacgatgggg atgacgatga tcgccagcgc
     1621 cagcgcaccc gcccagccgg agaagtgctt gacctgtatc acatagactt cgtagacgaa
     1681 caggccgatg acgatggaag gggcggagag caacacgtcg ttgatgaagc gggtgatcgg
     1741 ggccagccag ccgcgatgac cgaactcggc cagataggtg cccgcgagaa tgccgatcgg
     1801 cgtgccgatc agcgtcccca gcacggtcat catcaaggtg ccggcgatgg cgttgagcag
     1861 gccgccggta cttcccggcg gaggggtcga ctgtgagaag acgatggggc ccaatgccgg
     1921 cagcccgtac accagcaggg tccacaggat ccagcacagc cagaacaggc cgaatgccat
     1981 ggccagcatg gagaccacca gcccggtggc attgacgata cgacggcgga tatagagatt
     2041 tagcatgatg gttacgatgc ctggccctcg cgtttgccca gcttgtagag catgtagcgc
     2101 gcgagagaca agaccacgaa ggtgatgaag aacaggatca ggccgagttc gatcagggat
     2161 gaggtgtata gctcgccgac ggcttcggta aattcgttgg ccagtgccga ggaaatactg
     2221 ttgccgggca tcagcagcga cttgctcaga tcgtgcgcgt tgccgatcac aaaggtgacg
     2281 gccatggtct cgcccagtgc gcggcccagc cccagcatga tgccgccgac cacgccgatg
     2341 cgggtgtagg gcagtacgac ttgccacacg acttcccaag tggtcgcgcc tagaccgtag
     2401 gccgactctt tcagcagcgg ggggacgacg tcgaacacgt cacgcatcac cgacgcgatg
     2461 aatggaatca ccatgatcgc gaggatgatc ccggcggtca gcacgctgat gcccatcggc
     2521 gggccggtga agaatgggcc gagcatgggc agcgctccga ggttgtcgtt gatccagggt
     2581 tcgatatggt cggcaaacag cggggcgaag atgaacagcc cccacatgcc gtagatgatg
     2641 ctgggaatgc cggccagcag ttcgattgcg atgcccagcg ggcgacgcag gatgcgcggc
     2701 gacaattccg ttaaaaagat ggcgatgccg aagctgaccg gaatggcgat caacagggcg
     2761 acaccggagg tgacgagtgt gccgatgata ggcaccagcg caccgtattc atccgtgaca
     2821 gggttccact ccgcactggt caggaaaccg aaaccgaatg catggatggc aggcatgctg
     2881 ccgattacca gagagccaat gatggcggcg agcagcacga gtacagcaaa ggcggacaga
     2941 cgagtcacgc cgcgaaacag catgtccagc cagacctggc gcttgaggcg cgcaccgtgc
     3001 aagaacgata tagacataat tatagagtat cgttgaattt caaaaggggg catgacgccc
     3061 cctttgaatt atatcgtgga tacgtgctta gttcagcacg ttcgagcgat cgttacttcc
     3121 agattgcctt gccgctggca tccttgatct gagccttcca ggcggagcgg atcatcttgg
     3181 tgacgttgtc cggcatcggg atgtaatcca gatcggcagc catcttgttg ccgttgccgt
     3241 aggcccagtc gaagaacttt aacacttcag cagcattggc ggcattgtcc tggctcttgt
     3301 ggatcaggat aaaggtcgcg ccggtgatgg gccagctttc cttgcccggc tggttggtca
     3361 ggatctcgta gaaacccttg tcggcatccc acttggcatt ggcggcggca gccttgaagt
     3421 tgttctcggt tggggcaacg aactggcctt cgcgattctt caattgtgcg tagttcatct
     3481 tgttctgcag cgcgtaggca tattctacgt agccgatgga gcccttgatg cgttgcacgt
     3541 aggaagcgac gccttcgttg cctttgccgc cggtgccgac cggccattgc acggcggagc
     3601 ctgcacctac gccacgcagc cactcggtgc tgacctgcga caggtaggtg gtgaagatga
     3661 agctggtgcc ggagccgtcg gcgcggtgta ccacggtgat gctttggtcc ggcagattga
     3721 tgcctttatt gtctgccgcg atcttgggat cgttccactt ggtgatcttg ccctgataaa
     3781 tgccggagag cgtatcgccg gtcagtttca gttggcccgc agcgatgcct tgcacgttga
     3841 tgacaggtac cacgccgccg actacggtcg gaaactgtgc caatccggcc agacccaact
     3901 cgttcgggtt cagcggggcg tcagatgcgc cgaaatccac ggtcttggcc ttgatctgct
     3961 tgatgccgcc gccggaaccg atggattgat agttcatcgc aacgccagtc tgagccttgt
     4021 aagcttcagc ccattttgcg taaacaggat aggggaatgt cgcacctgca ccggtgatgg
     4081 tcgcggctgc cagacttgcg cctgcatagg ccataacggt agcggcggtg atgccaataa
     4141 cgagttgctt gagtttcata tgtatctccg tagtgtcgag tgtgctgctg cttgggggcc
     4201 atttaaatca acaatgccta gaggcggttc gcatcgtagt tcttttgtgt gacaggattg
     4261 ttacactatg caaccatttg ttatttaagt aaatagtttg cattgctgtt caggcgcgac
     4321 gtcggcaaca gcaccagcag gtctgcggtg ttgaatgccg gatcccaggc gggttcgccg
     4381 cagatatagg cgccgaggcg caaatagcgc ttgagcagca gcgggatagg cgcatccaga
     4441 tagggattca atgcctccag cggcagcggg ttgcgcggaa agacgcggta ttcgatcggc
     4501 gcgccgtaga tgcggtgcag cttgcggtag aggctcgcgg cgacatggcc gccatcggcg
     4561 atgccgatgc tgacgcagcc gatcaggtgc tcgtggcggt tctgtcgcag attttgggcg
     4621 atgccatccc acagccggtt gagcgtcgca ccgtcgcgat agtcgcgatg aatgcaggtg
     4681 cggccggctt ccgccatgcg tccgcgcaga tgcagcaaac gagtcaggtc gaattgggtc
     4741 tgcgcataat agccgccgat cttttgcgcc tgatccggcg cgagcaggcg gcatgtgccg
     4801 accacgttgt tgtcgccgct ttcgcgtacc agcagatgct cgcagtattt gtcgtaaatg
     4861 tcgcgatcca gtcctttctg ggaatggggc aggttcgcac ccatctcatc gcggaatatc
     4921 tggtagcgca atcgttgcgc ctcaagtatt tcatcgctat tgcgagccag tccggcgatc
     4981 aggcgaggtt gggcggcgga ttggacggtt cggtcgagtt ccagcatggc aggctccgga
     5041 tcggtttgag gatgccttta gtctatgggg gctctgttgc ggcgagatga cgaaaatgtg
     5101 aaggatgtgt gaattcccgt cggctggttg gtgttgggcg ccgtggtcgg gtattgcgga
     5161 gactgggggc ttacaagatg gaaggctcgg tcagcaaagg ggaagactgt tctgccggcg
     5221 tgtctggttg caggtatcgc tgtgtcgtcg ttcttttgag ttcctgtgca atggcttctt
     5281 gtgcagttcg tgccaatacc cggcggtttg caccggccgt tgtcagcgca ggcgtaaata
     5341 ccagcaaggc attgaaacgg gggctgcgca gcatgcgcca gacggagcgg gggagtgtca
     5401 tgtcgccgat gaaggcggca tgatcgctgc gttgtccatc catgtcctga tagcgcaggg
     5461 cgatcgggca caggcgaatg ccggcatcga tggccggttg gatcagtgcc gagtggaagt
     5521 gtccgacctg acggccgtct gtcgcggggc cttcgggaaa caaggtcacg caggtgcctt
     5581 gctgcagcag ggtttcgatg cgccggttga ccgagttggc atgcgggcgc atggcgcgtt
     5641 cgacaaaaat gctgccgccg cgctggcata accagccgat gagcggccag ttgcgcactt
     5701 ccagatccgt gatgaagcgc gatgggtgga tcgcattcag caccaggctg tccagccagg
     5761 aaacatggtt ggctacgatc aggcaggcgc catcgctgcg tgccggctgt gtgccttcga
     5821 tctggatgcc gatgtggaga atggacagca atcgtttgct ccatgacttc agcaagcgcc
     5881 gtcgtctgcc ggtggtgagg tgcgggtaga cgattgccag cgccattcca tatgccaggt
     5941 gcggcatcag cagggcgccg cgcatcaggc tggagagcag ggtcggcctg ttatgcataa
     6001 ctggcctccg ttacttcatt ggaattcaat tcgcgccgtt cgacgaacag gctgttgccg
     6061 ggttttttct tggccatctt tttggcgacg gtggcggctt ccgatatttc gtggtgcgaa
     6121 tggaacatgc cgggggtgac gcagaccacg cctatcgaga gtgtgggcag cgggtggtgg
     6181 acgacacggc cctgacggtc ttcactggca tagccgccga tggcgcgatg cgcttcctcg
     6241 aataacaccg aggaggtctg cgcaaatgag ctgagcgcct gattgcagcg cttctcccag
     6301 tctgtgctgc gcatgatcat gatgaagtca tcgccgccga tatggcccag caggtcgtgc
     6361 ctgggatcgc atgcccaatt gagcaggcgt ccggtgaatt ggatcgcctc gtcgcctttg
     6421 cggtaaccgt atacatcatt gaaaggcttg aagtggtcga tgtcgcaata gcaaaccgtg
     6481 aaggcctgat tgctgtgcag cagatgctcg atatgttcgt tgatgggcac gttgccgggc
     6541 aataaggtga gcgggttggc ataacgtgcg ttttcgatct gcatcttggt gatctcgcgc
     6601 aacagatcct gcgcattgcc gaggccgata tagcgtccct gttcggtgat gatgaagccg
     6661 ctgacgaagt gggagctctc cgtttcggtc aggaagtcgc tcaactcatg gatgggtgtg
     6721 gatttttcga ccagcagcgg ctcgatgttg atgcaatgcg aggacatatc cttgcaatgc
     6781 gcgccgccca tgcaggtgga gcagggcttt ttgcccagca ggtcgcgacg gaaggggtgg
     6841 acgaagcagg agaaatattg ctgtcgatcg atcaggccga ccgggatgcc acccttgacg
     6901 atggggatgg cgcgcaggct ctcgtcttcc gtaaagcgcg tgaaaatgtt ctcgtgcaac
     6961 gtttcgggat gcaccggttc cacgtaacgc tgtaacttct ctgtggtgac ctgacgcgag
     7021 gaaatgatct ggttggcgtt atggcacggt gggttggcga gattcacctg tgaactgacc
     7081 tcaaccggag cgaccagaga cggggttggg gtggggcggg cgataaaata gccttggcca
     7141 aagtcgatgc cgagatcgca aaccgtcctg aattcggctt cggtttcgat gccttcggca
     7201 atgaccagcg tgccggagct ttgtgctatc tgctggatgg agcgcaggaa ttgctgtttg
     7261 agcggatcgg cattgatgcc ctgcacgaaa tgcatgtcta ccttgacgta ttccgggcgc
     7321 agttcggacc agaggcgcag gctggaaaag cctgcgccca gatcgtcgat tgcaatctgg
     7381 aagcccatgc tgcggtagtg cagcagcgca ttgcgcatgt tctcgaagtc gaaagtgggt
     7441 tgattctcgg tgatctcgat gatgacctgt ttggggtcga tgccaatctt ttctagatag
     7501 ctcagggtct gaccattctt gaagctgggg tgcgtcagtg tgtccgggct gacgttcagg
     7561 aacagcttgc cgggaagtct ctgggcggcg aaggcttcca tgacgatctg gcggctcagc
     7621 atctcgactt ccagagcgag gtcttgctgc ttcgcggcgg cgaacagatt aaggggggaa
     7681 tgcagcgggc tgtctgccgg gccgcgtatc aggccctcaa agccgaggaa caggccgttc
     7741 gatatatcca tgatcggctg gaacagggca gaaagggcgc gccgctcgag tattccttgt
     7801 agtgttttca cgctgagact cctgtgtgtg gatgcgcgcc gaaggatagt gagcgaatgt
     7861 tgcaagctga ttaaggtgat gttgcagatt ggttacaatg cggtccctga aaattcaggt
     7921 tgaacattct ggattccttc ggtagaatcc gcgctctttt gaaaatcggt tggagtagaa
     7981 ccatggcacg tgtatgtcaa gtgacgggta aaggcccgat gactgggaac aatgtttccc
     8041 atgcgaacaa caaaaccaag cgtcgtttct tgcctaacct gcaacgtcgc cgcttctggg
     8101 ttgaaacaga aaaccgttgg gtgagcttgc gcttgaccaa cgcagcgctg cgcactatcg
     8161 acaagaacgg catcgaagcc gtattgttcg agatgcgtgc tcgtggcgaa aacgtttaag
     8221 gagctagcaa atgcgcgaaa aaatcaaact tgaatccagt gccggtaccg gtcactttta
     8281 tacaacggac aagaacaaga agaccactcc ggaaaagctc gaaatgaaaa agttcgaccc
     8341 ggtggttcgc aagcacgtta tgtacaagga aactaaactg aagtgattca gtttgttact
     8401 ggccaataaa aaacccgctc aggcgggttt tttattggcc atccagaatt tgtgcggcaa
     8461 gaggtcgatt gatctattct tggaagcaat ttagccagcc cattatttcg ccacaagaaa
     8521 actatgcata cacatgatct gtcccgctgg acgcaccagc acgtatttaa tcagggaagc
     8581 cctgctgctg agcgcagcac ccgtgcggtg atgtggatca ccgccgcgat gatggtggta
     8641 gagattgccg ctggctggtg gtttaactcg atggcgctgc tggcggatgg ctggcacatg
     8701 agttcccatg cttttgcgat cggattgagt gcctttgcct atgcggccgc gcggcgctac
     8761 tcaacggatc caaggtttgc cttcggcacc tggaagatcg agattctcgg cggcttcgcc
     8821 agcgcgattt ttttgctggg cgtcgcggcg atgatgatct tcggatcggt cgagaggttg
     8881 ttctcgcctc agcagattca ctatcaggaa gcgatcatcg tcgccgtcat cggtttagtg
     8941 gtcaacatcg tctgcgcgaa gatactgggt catgctcatc acgggcacga tcagcacagc
     9001 catgcccatg cagatcacga ccatcaccat catgatctga acctgaaatc ggcctatgtg
     9061 catgtcatcg cggatgcggc gacttccgtg ctggccatcg ttgcgctgat cggcggatgg
     9121 ttctatggct ggtcctggct ggatcccgtg atgggcatcg tcggcgcgat actggtcgcc
     9181 gtttgggcga aggggttgct gatcgagacc g
//
LOCUS       DASUIV010000095         3553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1232414, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000095 DASUIV010000000
VERSION     DASUIV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232414"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..929
                     /locus_tag="VFW59_03535"
     CDS             405..929
                     /locus_tag="VFW59_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5363317.1"
                     /translation="MRRLREQAGISQEELAFRTSLDRTYVSMLERGRRQPSLATVEKV
                     AEALGMKASVFLQLVENDCIGQKPLNVAASEAQDKQMKYLFSGQANDAGELFLVELEE
                     VGEGAFLVRYGTMTSTSGLERYPKDGSSMMPLEAVQFLGRLRTCEQMPESCRADYKAW
                     LTQLEIDFINQRIG"
     gene            complement(935..1276)
                     /gene="fdx"
                     /locus_tag="VFW59_03540"
     CDS             complement(935..1276)
                     /gene="fdx"
                     /locus_tag="VFW59_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEX5363318.1"
                     /translation="MPNIIILPHVELCPEGSVIEAKSGVSICDNLLENGIDIDHACDK
                     VCACTTCHVIVREGYNSLPAAEDNEEDLLDKAWGLEPNSRLSCQAIVGTSDLVVEIPR
                     YSINMAKEGKR"
     gene            complement(1293..2126)
                     /gene="motD"
                     /locus_tag="VFW59_03545"
     CDS             complement(1293..2126)
                     /gene="motD"
                     /locus_tag="VFW59_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HEX5363319.1"
                     /translation="MARKKKHEEHENHERWLVSYADFITLLFAFFVVMYAISSINEGK
                     YKILSDSIVEAFKSAPTSEKIIYVQAPTPQGAVESGKPIPQIIPMQKPDTPAETEKQQ
                     RRENMKSVANDLLKVMEPLVKGGQVKVTESNRGITIEINASLLFETAKADLNPASAEV
                     LNAVAKVLATDTHQIQVEGHTDNLPMKSPVFPSNWELSSARASSVIRLFADNGVGGDR
                     LVAIGYADNRPVDSNDTPEGRARNRRVKVMILAENQGTVQDLSLDPDTRPSAATTPAT
                     P"
     gene            complement(2145..2900)
                     /locus_tag="VFW59_03550"
     CDS             complement(2145..2900)
                     /locus_tag="VFW59_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEX5363320.1"
                     /translation="MNKLPFDISTLLGILLALGAIIGGQILEGGHINTLMQLTAFIIV
                     IGGTMGAVMIQYSLPSFLLGIRLIKWAFVPPRIHLEEVIEQITSWGNTARRGGLLSLE
                     PILVEMQDAFMKKGLQMLVDGAEPEKLKESLEVEMDTYDDHYRLAAKVWESAGGYAPT
                     IGIIGAVMGLIHVMGNLSDPSKLGGGIAVAFVATIYGVGSANLLFLPISSKIKSIIGQ
                     EMVKRQMVIEGLVAIANGENPRLIDTKLRGFIA"
     gene            complement(2953..>3553)
                     /locus_tag="VFW59_03555"
     CDS             complement(2953..>3553)
                     /locus_tag="VFW59_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEX5363321.1"
                     /translation="DDLMQAGMMGLLDAMEHYDESHGAQFETYASQRIRGAMLDELRE
                     IDWVPRSVRKNARDIEHTITALQQKLGHPPLEQEIANALGVELAEYQSMLADARGHQL
                     VYYEDFHNEDGDPVELNLPDDRPNPFELLQDNGMRQALIDAIDGLPEREKMMMAMYYQ
                     EDLNLKEIGTIMGVSESRVCQIHTQAVMRLRGKLKAWIG"
BASE COUNT          754 a          894 c          995 g          910 t
ORIGIN      
        1 actgcaaaat gctgcttccg tggccggcct gatgctgacc accgactgca tggttgcaga
       61 actgcctaag gatgacgctc cggcaatgcc gggtggcatg ggcggtatgg gcggcatgga
      121 catgatgtaa tgtccgctaa agatcgggat gtgagccctg gtctttgaca ctcaaaaaaa
      181 gccccgcatt cgcggggctt tttcttttta taaatcaaaa agttctagtc gaacttattc
      241 ggaaataaaa cattcaaatt ggaatagaag tgcagacagt tgtccgtggc tgcgggatat
      301 ttgtccatgt aacctcgcag ctccctttta aagggataag gaaatccttg atcatgcctt
      361 tatccaatca tgcaaattgc ttgtcagtcc tatacggagc ggtcttgagg cgtttgcgtg
      421 aacaggcggg catcagccag gaagaactgg ctttccgtac cagtctggat cgtacctatg
      481 tatcgatgct ggaacgtggc cgcaggcaac ccagcctggc aacagtcgaa aaggttgcgg
      541 aagcattggg gatgaaagct tccgtattcc ttcagctagt tgaaaatgac tgcattgggc
      601 aaaagccgct gaacgttgca gcatcagaag cacaagacaa acaaatgaaa tatctgttta
      661 gtggccaagc gaatgatgct ggcgagctgt ttttggtcga gttggaagag gttggagaag
      721 gtgcgtttct ggtgcgctat ggcacgatga catcaacgtc aggcttggag cgatacccaa
      781 aggatggctc atcgatgatg ccgttggaag ccgtacagtt tcttggccgc ttgcgaacct
      841 gcgaacaaat gccggaaagt tgccgggccg attacaaggc gtggctgacc cagttggaaa
      901 tcgatttcat taaccagcgc atcggctgat gcacttaacg tttgccttcc ttcgccatat
      961 tgatggaata gcgaggaatc tccactacca agtcactggt gccgacaatc gcctggcagg
     1021 atagccggga attgggttcc agcccccatg ccttgtccag caggtcctct tcattgtctt
     1081 ccgcagcagg caggctgtta tatccctccc gtacgatcac gtggcaagtg gtgcaggcac
     1141 agaccttgtc gcaggcatgg tcgatgtcga tgccgttttc cagcaggttg tcgcagatgg
     1201 aaacgccgga ttttgcctcg atgactgagc cttcagggca gagttcgacg tggggaagaa
     1261 taatgatgtt tggcatttgg cttatactaa atttatggcg tggccggggt tgtggccgct
     1321 gatgggcgag tgtcgggatc tagcgaaaga tcctgtactg ttccttggtt ctcggccagt
     1381 atcatgacct tgacgcgacg gttgcgtgca cgcccttcag gcgtgtcgtt gctgtcgaca
     1441 ggacggttgt cggcgtagcc aatggctacc aggcggtcgc caccgactcc attgtcggca
     1501 aacaagcgta tgacactact ggcgcgggcg ctggatagtt cccagttgga tgggaaaaca
     1561 ggcgacttca tcgggaggtt gtccgtatgt ccttccactt ggatttggtg ggtgtcggta
     1621 gccaatacct tggctaccgc attgagcact tctgcagatg ccgggttgag atcggctttt
     1681 gctgtctcga acagcaggct ggcattgatc tcgatggtaa tgccgcgatt gctttccgtt
     1741 accttgacct gcccaccttt taccagcggt tccatcacct tgagcaaatc gtttgcgacc
     1801 gacttcatgt tttccctgcg ctgttgcttt tcggtttccg ctggggtgtc cggtttctgc
     1861 atggggatga tttgcggaat cggtttgccg ctctcgacag ctccttgtgg tgtaggagct
     1921 tgtacataga tgattttttc gctggtgggg gcgctcttga aagcctcgac gatagagtcc
     1981 gagaggattt tgtatttgcc ttcgttgatc gatgagatgg cgtacatcac gacgaagaac
     2041 gcaaataaca gcgtaatgaa gtcggcatag gataccagcc agcgttcgtg gttctcgtgc
     2101 tcttcgtgtt ttttcttgcg cgccatggcg atgccggtgc aggtttaggc aatgaagccg
     2161 cgcagcttgg tgtcgatcag tcgcgggttt tcgccattgg caatggctac cagtccttcg
     2221 atgaccatct ggcgcttgac catttcctgg ccaatgatcg atttgatctt gctcgagata
     2281 ggcaggaaca gcagattggc cgaaccgacg ccgtaaattg tggcgacgaa ggcaacggca
     2341 atgccgccgc caagcttgga tggatcggac agattaccca tgacgtggat cagtcccatg
     2401 acggcaccga taatgccgat ggtaggggca tagccgccgg cagactccca gacctttgcc
     2461 gcgaggcggt aatggtcgtc ataagtgtcc atctcgacct ccagtgactc cttgagcttt
     2521 tccggttcgg cgccgtctac cagcatctgc aggcctttct tcatgaaagc atcctgcatt
     2581 tcgaccagta tgggctccag cgataacaag ccgccacgtc ttgcggtatt gccccagctg
     2641 gtgatttgtt cgatgacttc ttccagatga atgcggggag ggacaaatgc ccatttgatc
     2701 aggcggatgc cgagcaggaa gctcggcaat gaatactgga tcatcactgc gcccatggta
     2761 ccgccgatga cgataatgaa tgcagtgagc tgcatcaggg tgttgatgtg cccaccttcg
     2821 agtatctgtc cgccgatgat ggcgcccagc gcaagcagaa tgccgagcag ggtactaatg
     2881 tcgaaaggga gcttgttcat ggatgaattc taaccgattc gtgttcttgg atattggtat
     2941 acattcttaa ttttaaccaa tccacgcctt tagcttgccg cgtagccgca tgacagcctg
     3001 tgtatggatc tggcaaacgc gcgattcgga aacgcccatt attgtcccga tttccttcag
     3061 gttgaggtct tcctgataat acatggccat catcattttt tcgcgctctg gcaggccgtc
     3121 gatggcgtcg atcagtgcct gtcgcatgcc gttgtcctgc aacagttcaa acggattggg
     3181 gcgatcatcg ggcaagttaa gctcaaccgg gtcgccatcc tcgttgtgaa agtcttcgta
     3241 atataccaac tggtggccac gcgcatcggc cagcatcgat tgatattcgg ctagctccac
     3301 tcccagggcg ttggcgattt cctgctccag tggtgggtgt cccagctttt gctgtaacgc
     3361 cgtaatcgtg tgttcgatat cgcgtgcatt cttgcgtacc gatcgcggaa cccagtcgat
     3421 ttctcgaagc tcatccagca ttgctcctcg aatgcgctgt gatgcatagg tttcaaattg
     3481 ggcgccatgg gactcgtcgt aatgttccat ggcatccagc aagcccatca tccccgcttg
     3541 catcaagtca tcc
//
LOCUS       DASUIV010000096         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_952376, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000096 DASUIV010000000
VERSION     DASUIV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952376"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFW59_03560"
     CDS             <1..401
                     /locus_tag="VFW59_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5363322.1"
                     /translation="ANTFAMMYGSGVNIMECITNSRDVVNNLVVAKSLDDVMLEIESG
                     KNLTQSFQSTGMFPPLVIRMLKVGEATGSLDKALLNVSYFYDRDVKDSIKKVQIMIEP
                     TMTIVLGALLGWVMLSVLSPIYDIIGKVNF"
     gene            401..1882
                     /locus_tag="VFW59_03565"
     CDS             401..1882
                     /locus_tag="VFW59_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363323.1"
                     /translation="MKNALLLLLSANHLHAQLIAGGKITAQQEFSDTPESRNEFTAFV
                     SRINHPAYLLVDLIEEDFRQETIPHLIGSSRNALLQRKFEQFYRNTPFRQATLLQRQK
                     TGRRDDDMLFSALTNPALITPWLNILLAQKVPLAGIYSVPQLSAPLIEDHPSDYLLLI
                     SWEKSAGLRQTFFSQHRLQISRLTPINADQTFQDAVLTELPRTFQYLKSLSLLPGGQT
                     LDVRLLGHRSDLSELKAALPVSLDMRYDFVELIELARRHCIEQHCTDSDASQIFLHHL
                     AAKPPQTHYANANHIHYYTLWRLRHALNWISAALLLGALTGGMIGIWQGGWNTRDARA
                     MNGEAQKILNEARQITDTFPNTHVPASDMKASVSIMHDLERQTLQPETVMRPLSNVLD
                     RYPQIELDELEWAMDTVPNTASGTPPQVITLKGRLVGFASNYRRAFEHLKRFGRDLEI
                     QGYHVTVLSEPLDISPSGSIADRREATPDALDFSLKLLRRPPT"
     gene            1879..2481
                     /locus_tag="VFW59_03570"
     CDS             1879..2481
                     /locus_tag="VFW59_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363324.1"
                     /translation="MKFSLSDLPMMRWSMLAICASTLFSGILLYGSGEYAGQKLKDKN
                     SAQAQLNNARNQLTAAREDRKNMAAYADEYAALAEGGIIGDGTRMDWLEGLENLRRQQ
                     LVADFRYTIAPQKAYTPVQPINSEMFDIQYSEMKLEFELLHEGQLLNFFSALRSQING
                     HYQLEGCTLQRPAANSGLATGLKAVCRGGWITLKNRNATP"
     gene            2586..2930
                     /locus_tag="VFW59_03575"
     CDS             2586..2930
                     /locus_tag="VFW59_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363325.1"
                     /translation="MPFTSIICALALTTSVPASAEELGRLFFTPEQRAQLDIEHARNI
                     DPADSPNTLSVNGIVQRHGGDRTVWINGVPQVAGKSDEHAPESVPVAVPGQSRPVKVK
                     VGQRVMLNPARE"
     gene            2940..4136
                     /locus_tag="VFW59_03580"
     CDS             2940..4136
                     /locus_tag="VFW59_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363326.1"
                     /translation="MSKTASPPGRQNGLALVAILAILVVGGTAVLIGSLSITALHNAQ
                     QEATSAALAQAKDGLIGYAISSENAGGATARPGNFPCPDTDAPGTSGYGTEQASCAAG
                     AIGRLPWKTLGLPELRDGSGEPLWYALSGNFRKSAARINSDTRGTLLVYDHDGSTLLT
                     PSGNEAVAIILAPGNITGSQQRNSSTDKVTASNYLDAAKGQNNATTGGPFIAADKSET
                     FNDRLLVIRTRDFIPTVEKRVAKELKSILENYRAANGVYPYPAPFSSCYTNSTCTSDT
                     NTCRGRLPYTALPQDWGGSYSLPQTASGSPWFINNRWFTVIYYSAGTSRLSTAPAGCN
                     ATLDVSGSSTPALFFLPGTPLGGVTRTYPNNNLSWYLEDAENQNMDNNYVAPTTNSND
                     HLHALP"
     gene            4123..4755
                     /locus_tag="VFW59_03585"
     CDS             4123..4755
                     /locus_tag="VFW59_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5363327.1"
                     /translation="MRSHNHARGFTLVEMAIVLLIIGLLLGGLIPTLSAQMETQRISE
                     TRKQMSEIKDALFGYAVMHGRLPCPATTTDPANTSYGEADSSCANPASNNYLPWKTLG
                     VAETDAWGSKWLYRVDPAFASATPFALTTSPSASLLIQNSAGITLTSSTEPPIAIVFS
                     TGKNLSADGQNASFDATYQSDVPNPSFDDITAWISRPILFNRMVTAGKLP"
     gene            complement(4752..>4942)
                     /locus_tag="VFW59_03590"
     CDS             complement(4752..>4942)
                     /locus_tag="VFW59_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5363328.1"
                     /translation="EFLQGGATEGGLDDVEIDAQIAARIAAKQAKDFAEADRIRKALL
                     EAGIVLEDSASGTTWRRV"
BASE COUNT         1148 a         1609 c         1299 g          886 t
ORIGIN      
        1 tcgccaacac cttcgcgatg atgtacggat cgggcgtgaa catcatggag tgcatcacca
       61 attcccgcga cgtggtcaac aacctggtgg tcgccaagag cctggacgat gtcatgctgg
      121 aaatcgaatc cggcaagaac ctcacgcaaa gcttccagag caccggcatg ttcccccccc
      181 tggtgatacg catgctcaag gtgggtgagg cgaccggctc cctggacaag gcgctgctca
      241 atgtcagcta cttttatgac cgcgatgtga aagactccat caagaaggtg cagatcatga
      301 tcgaaccgac catgaccatc gtgctgggcg cgctgctcgg gtgggtcatg ctgtccgtgc
      361 tgtcgcccat ctacgacatc atcggcaagg tgaacttctg atgaaaaatg ccctgctgct
      421 tctgctgagc gccaaccacc tccacgccca actcatcgcg gggggcaaga tcacggcaca
      481 gcaggaattc tccgatacac cagaatcacg caatgagttc accgcattcg tgagccgcat
      541 caaccatccg gcgtatctgc tggtcgacct gatcgaagag gacttccgtc aggaaaccat
      601 cccccacctg atcggcagct cccgcaatgc cttgctgcaa cgcaaattcg aacagttcta
      661 tcgcaacaca ccgttccgtc aggccacgct gttgcagcga cagaaaaccg gacggcgcga
      721 cgacgacatg ctgttctcgg cactgaccaa tcctgcgctg atcaccccct ggctgaacat
      781 cctgctggcg cagaaggtgc cactcgccgg gatttattcg gtacctcagc tcagcgcgcc
      841 gctgatcgaa gatcacccgt ccgactacct gctgctgatc tcctgggaaa agtcggccgg
      901 attgcgccaa acttttttca gccaacaccg gctgcagata tcccgcctga cgcccatcaa
      961 cgccgatcag acctttcagg atgcggtatt gactgagctc ccgcgcacct tccaatacct
     1021 gaagagcctg agcctgctcc ccggcggcca gacactggat gtgcgcctgc tgggacatcg
     1081 ctcagacctg agcgagctca aagccgcgtt gccggtgagt ctggacatgc gctacgactt
     1141 tgtggagctg atcgaactcg cccgccgaca ctgcattgag caacactgca ccgattcgga
     1201 tgccagtcag atattcctgc accatctggc agcgaaaccg ccgcagaccc attacgccaa
     1261 tgccaatcat atccattact acacgctgtg gcggttgcgc cacgcgctga actggatcag
     1321 cgcggcactg ctgcttggcg cactgacagg tggcatgatc ggcatctggc aaggcggatg
     1381 gaatacccgc gatgcgcgcg ccatgaatgg cgaggcgcaa aagatcctga atgaggcgcg
     1441 gcagatcacc gacaccttcc ccaacaccca tgttcctgcc tcggacatga aggcctccgt
     1501 ctcgatcatg cacgatctgg agcggcaaac cctgcagccg gaaacggtca tgcgcccgct
     1561 cagcaatgtg ctcgaccgct atccccagat cgaactcgac gaactggaat gggccatgga
     1621 cacagtaccg aacaccgcct ccggcacccc accccaagtc atcacactca agggacgatt
     1681 agtcggcttc gccagcaact atcgccgcgc attcgagcat ctcaaacgtt tcgggcgcga
     1741 tcttgagata cagggctacc atgtcaccgt gctgagcgaa ccgctcgaca tcagcccgag
     1801 cggcagcatc gccgaccgac gcgaagcaac accggatgcg ctcgacttct cgctgaagct
     1861 actccggagg ccgccaacat gaaattctct ctctccgatc tgcccatgat gcgctggagc
     1921 atgctggcaa tctgcgcctc gacgttattc tccgggattt tgctgtatgg cagcggcgaa
     1981 tatgcggggc aaaaattaaa agacaaaaat tccgctcagg cccagctcaa caacgcgcgc
     2041 aaccagttga ccgccgcgcg cgaagaccgc aaaaacatgg cggcctacgc cgatgaatat
     2101 gccgcgctgg cagagggcgg catcatcggc gacggcaccc gcatggactg gctggagggt
     2161 ctggaaaacc tgcggcgcca acaactggta gccgattttc gctacaccat cgcaccgcag
     2221 aaagcctaca cccctgtaca gccgatcaac agcgagatgt tcgacatcca atacagcgaa
     2281 atgaaactgg aattcgaatt gctgcacgaa gggcagctcc tgaacttctt ctccgccctg
     2341 cgcagccaga tcaatggtca ctaccagctc gaaggctgca ccctgcagcg ccccgccgcc
     2401 aatagcggcc tggccacggg actcaaggcc gtatgccgtg gcggctggat caccctgaaa
     2461 aaccggaacg caacgccatg aacggaattc ttgaaatcgt cctatatcca ttcgcccaaa
     2521 gggcacggca cgccgtgccc ctacgggtgt gttgctcggg ccgaattgta agggcacggc
     2581 gtgccgtgcc cttcacgtcc ataatctgcg ccctcgccct gacgacctcc gttcccgcca
     2641 gcgccgaaga actgggccgg ttgttcttca caccggaaca gcgtgcccaa ctggatatcg
     2701 aacacgcccg caacatcgat cccgcagata gccccaacac cctgtcggtc aatggcatcg
     2761 tgcaacgaca tggcggcgat cgcaccgtct ggatcaacgg cgtaccgcaa gtcgcaggca
     2821 agagcgacga gcacgcgccg gaaagcgtcc ctgtcgctgt ccccggacag tcgcgccccg
     2881 tcaaagtcaa agtcggacaa agggtcatgc tcaatcccgc gcgcgaatag acacgcacca
     2941 tgagcaaaac cgccagccct cccggcagac aaaacggcct cgctctggtg gcgatactgg
     3001 cgatactggt cgtgggtggc accgccgtgc tgatcggatc actcagcatc acggcactgc
     3061 acaatgcaca acaggaagcc accagtgccg cactggcaca ggccaaggat gggctgatcg
     3121 gttatgcaat cagcagcgag aatgccggcg gcgccaccgc caggccaggc aacttcccct
     3181 gccccgacac cgatgcaccc ggtacaagcg gctatggaac ggagcaagca tcatgcgccg
     3241 ccggtgcgat tggcaggctg ccttggaaaa cactcggcct accggaactc cgtgatggtt
     3301 caggggagcc gctatggtat gcactttcag gaaatttccg aaaatccgcc gcccggatca
     3361 acagcgacac gcgcggcacg ctgctggttt atgaccacga cggcagcacg ctgcttacgc
     3421 ccagcggcaa cgaagcggta gcgatcatat tggctcccgg caacatcacc gggagccaac
     3481 aaagaaactc aagcaccgat aaagttacgg caagcaacta tctcgatgcg gccaagggtc
     3541 aaaacaatgc gacgacaggt ggtccgttca tcgctgccga caagagcgag acgtttaacg
     3601 atcgcctgct ggttatccgc acacgcgact ttataccgac cgttgagaag agagtagcca
     3661 aagaactgaa aagcatcttg gaaaactatc gtgcagccaa tggggtctac ccgtaccctg
     3721 ccccattcag cagttgctac accaattcga cttgcaccag cgacactaac acttgccgag
     3781 gtcgcctgcc atataccgcc ctcccccaag actggggagg gagttattca ttgcctcaaa
     3841 cggcaagtgg ctccccttgg tttatcaaca atcgatggtt caccgtgatt tactacagcg
     3901 cgggaacaag ccgcctcagt acagcacccg caggttgtaa cgccaccctt gacgtttccg
     3961 gcagcagcac acctgcgcta ttcttcttgc cgggaacgcc actaggcggc gtaacgcgca
     4021 cctaccccaa taataattta tcttggtatc tggaagatgc agaaaatcag aacatggata
     4081 acaactatgt tgccccaacc accaactcca atgatcacct ccatgcgctc ccataaccac
     4141 gcaagaggat tcacccttgt cgaaatggcg atcgtgctgc tgatcatcgg cctgctgctg
     4201 ggtggcctga tcccgacgct gtccgcacag atggaaacgc aacgcatcag cgagacacgc
     4261 aaacagatga gcgagatcaa ggatgcgctg tttggatatg ccgtcatgca tggcagattg
     4321 ccgtgcccgg cgaccaccac cgatcctgcc aatacgagct atggcgaggc ggacagcagc
     4381 tgcgccaatc ctgcatcaaa caactatctg ccctggaaga cgctgggcgt cgcggaaacc
     4441 gatgcctggg gcagcaaatg gctataccgg gtagatccgg ccttcgcatc cgcgacaccc
     4501 tttgccctga cgacatcgcc atcggcctcg ctgctcattc agaacagcgc gggcatcacg
     4561 ctgaccagca gcaccgagcc cccgatcgca atcgtatttt caaccgggaa gaatctttct
     4621 gccgatggcc agaatgccag cttcgacgcc acttaccaga gcgatgtccc caatcctagc
     4681 ttcgacgata tcacggcatg gatatcgcgc ccgatactat tcaaccgcat ggtgacggcg
     4741 ggcaaactgc cttaaacgcg ccgccaggtc gtgccggatg cgctgtcttc cagcacgatg
     4801 cccgcctcca gcaacgcctt gcggatgcgg tcggcctcgg caaaatcttt cgcctgtttg
     4861 gctgcgatgc gcgctgcgat ctgcgcatcg atctcgacat catccagccc gccctcggtc
     4921 gcgccgcctt gcagaaattc ct
//
LOCUS       DASUIV010000097         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_23367, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000097 DASUIV010000000
VERSION     DASUIV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23367"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..981)
                     /gene="pfkA"
                     /locus_tag="VFW59_03595"
     CDS             complement(10..981)
                     /gene="pfkA"
                     /locus_tag="VFW59_03595"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5363329.1"
                     /translation="MKHIGVLTSGGDAPGMNAAIRAVVRAASFHGVAVTGIERGYKGM
                     IDGAARPLGPRDVSGIISRGGTILHTARCHRFMEIEGRRQAAENLAKWGIEGLVVIGG
                     DGSYQGAARLHEEFGIRCIGLPGTIDNDIGGTDYTIGFDTAINTAMEAIDRIRDTAAS
                     HDRIFFVEVMGRRSGYVAMISGIAGGAEDILLPEVETSVDTLIERLRSGQQRGKQSSI
                     VIVAEGCEYGGAMALAKMVGERSEYKDNRITVIGHLQRGGSPSAFDRVLASQMGVHAV
                     EALIAGESGKMVGISGQKLQLRPLSDAWEQRTSFGMELLRIAHVLSI"
     gene            1408..2058
                     /locus_tag="VFW59_03600"
     CDS             1408..2058
                     /locus_tag="VFW59_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5363330.1"
                     /translation="MTQGKAIRLLIADDHSIVRRGLVQLFSLIDDIHVAGEVANGIEL
                     LDILRQGISFDILFMDMNLAGFGGLKLVSAIHSLHPSLPILIFTIHSDLIIARQAFQS
                     GASGYAIKGCTIGMLLVAIRTVAAGGRYVDPEMAEYMLLAKVAPKPVTPHQKLSEREL
                     HILKLFAQGLSGNEIARKLSISKKTVSTHKNNLMQKMNFQNIADLVLYAARYSLIE"
     gene            2124..3236
                     /locus_tag="VFW59_03605"
     CDS             2124..3236
                     /locus_tag="VFW59_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5363331.1"
                     /translation="MTPFPLRLSDLRQRLRELGCKPCHEDRLLRGWSQIGSYDRKGSP
                     AETFFPQALREALPAIEAELEGLARLRSKHPVSLYPTLSHFMGEGANAALRAPSNNDE
                     VARLLVELHDGQMVESVLLPRGGLCVSTQVGCAVGCVFCMSGRAGLLRQLGSAEIAAQ
                     VVLARRRRTVSKVVFMGMGEPAHNLDNVLEAIELLGAQGNIGHKNLVLSTVGDLRVFE
                     RLPLEKVKPALALSLHTTDAALRAKLLPHAPQIPIEELVERCEAYALATGYPVQYQWT
                     LIEGVNDSDAELERIAQLLSGKYALMNFIPFNEVDGLAYRRPSPERIASMVQTLKRHG
                     VLAKVRDSAGQEIEGACGQLRARAAKTKSDKTRASE"
     gene            3282..3419
                     /locus_tag="VFW59_03610"
     CDS             3282..3419
                     /locus_tag="VFW59_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363332.1"
                     /translation="MPKSHNAHRDSKKKPLLTPKEKRMAKHEKKHSHDVQPFIPPATT
                     H"
BASE COUNT          778 a         1182 c         1037 g          716 t
ORIGIN      
        1 tttcaggcgc tagatgctca gcacatgcgc gatgcgcagc agctccatgc cgaagctcgt
       61 gcgctgctcc cacgcgtcgc tgagcgggcg cagctgcagc ttctgcccgg agatgccgac
      121 catctttccc gattcgcccg cgatcagcgc ctcgacggca tgcacgccca tctggctggc
      181 gagcacgcgg tcgaaggccg acggtgaacc gccgcgctgc aggtggccga tcacggtgat
      241 gcggttgtcc ttgtattcgg aacgctcgcc gaccatcttc gccagcgcca tcgccccgcc
      301 gtattcgcag ccttcggcga cgatgacgat ggaggactgc ttgccgcgtt gctgcccgct
      361 gcgcagacgc tcgatcagcg tatcgaccga ggtctcgact tccggcagca ggatgtcttc
      421 cgcgccgccc gcgatgccgc tgatcatcgc gacataaccg ctgcgccgcc ccatcacctc
      481 gacgaagaag atgcggtcgt gcgaggcggc ggtgtcgcgg atgcggtcga tggcttccat
      541 cgcggtgttg atggcggtgt cgaaaccgat ggtgtagtcg gtgccgccga tgtcgttgtc
      601 gatggtgccg ggcaggccga tgcaacgtat gccgaactcc tcatgcagcc tggccgcacc
      661 ctgataggaa ccgtctccgc cgatcaccac cagaccttcg atgccccatt tcgcgaggtt
      721 ctccgccgcc tgtctgcgtc cctcgatctc catgaaacga tggcagcgcg cggtgtgcag
      781 gatggtgccg ccccggctga tgatgccgct gacgtcgcgc gggccgagcg ggcgggccgc
      841 gccgtcgatc atgcccttgt agccgcgctc gatgcccgtc accgccacgc cgtgaaagga
      901 ggccgcgcgc accacggcgc gtatcgccgc attcatgccg ggcgcgtcgc cgccgctggt
      961 caatactccg atgtgcttca tctcaactcg ttcatgcgtt tggacagggg cgcgattcta
     1021 gtggaaagac gagcgctcgg ccgactttac atctgttgta cgtctcgcag acggctagca
     1081 cagatatccc gctaaagaac ctaccaacct agacaggtta tttttccaca gaaacttagt
     1141 acatttcccg ggcgcgctca aacgccgaag aaacattgac caggcaattc gaaggccccc
     1201 cccattcgat tagccatgtc ccctgcaaaa atgatgagcc ctcaaaaagc caggtattca
     1261 cccggaacgc ttcatccgtg cccgcagaac tccatcaact aggactagtc ctaatttccg
     1321 gcagcaaaag agcgattgcc tgatagcttg cgcccaccaa gctttgagac catccggcca
     1381 atcaacaaag attgttaaaa gtaccaaatg actcagggta aggcgatacg attgctcata
     1441 gcagacgatc acagcatcgt acgcagaggg ttggtgcagc ttttttccct gattgacgac
     1501 attcatgttg ccggcgaagt cgccaacggc atcgaactgc tcgatattct ccggcaggga
     1561 atcagtttcg atattttatt tatggatatg aacctcgcgg gtttcggcgg cctcaaactc
     1621 gtctccgcga tccattcgct gcacccgtct ctccccattc tcatctttac catccatagc
     1681 gatctcatca ttgccagaca agcattccag agcggtgcca gcggctatgc catcaaggga
     1741 tgcactatcg gcatgctcct cgtggcaatc cgcaccgttg cggccggggg gcgctatgtc
     1801 gatcccgaaa tggccgaata tatgcttctc gccaaggtcg cccccaaacc cgtcactccc
     1861 caccagaaat tgtccgagcg cgaattgcat attttgaagc tgtttgcaca gggactcagc
     1921 ggcaacgaaa tcgccagaaa actgtcgatc agcaagaaga ccgtcagcac gcacaagaac
     1981 aacctgatgc agaaaatgaa ttttcaaaac atcgcggatc tcgtgcttta tgccgctcga
     2041 tattccctga tcgaataacc cctatccgca ccgcataccc gcctgggtta aaatccgccc
     2101 gccttcattc ccgtgccctt gccttgactc ccttcccgct acgactctcc gacctccgcc
     2161 agcgcctgcg cgagctgggc tgcaagcctt gccacgagga ccggctgctg cgcggctgga
     2221 gccagatcgg ctcgtacgac aggaaaggca gtcccgccga gacattcttc ccgcaggcac
     2281 tgcgcgaagc cctgcccgcc attgaagcgg agctggaggg gctggcgcga ttgaggagca
     2341 agcatcccgt ctccctctac ccaaccctct cccatttcat gggagagggg gcgaacgccg
     2401 cgctgcgcgc accttccaac aacgacgagg tcgcgcggct gctggtggaa ctgcacgacg
     2461 ggcagatggt ggagagcgtg ctgctgccgc gcggcggcct gtgcgtgtcg acgcaggtcg
     2521 gctgtgcggt gggctgcgtg ttctgcatga gcggccgcgc agggctcttg cgccagctcg
     2581 gcagcgcgga gatcgcggca caagtggtgc tggcacgtcg ccgccgtacc gtcagcaaag
     2641 tggtgttcat gggcatgggc gaaccggcac acaatctcga caacgtgctg gaagcgatcg
     2701 aactgctcgg cgcgcagggc aacatcggcc acaagaatct ggtgctctcc acggtcggcg
     2761 acctgcgcgt gttcgagcgg ttgccgctgg agaaagtcaa acccgcgctg gccctgtcct
     2821 tgcacaccac cgatgcggcg ctgcgcgcaa agctgctgcc tcatgcgccg cagataccca
     2881 tagaagaact ggtcgagcgc tgcgaggcct atgcgctcgc caccggctat ccggtgcagt
     2941 accagtggac gctgatcgag ggcgtgaacg acagcgatgc cgaactggag cgcatcgccc
     3001 aactgctatc aggcaaatac gcgctgatga acttcattcc cttcaacgaa gtcgacggcc
     3061 tcgcctaccg ccgcccctcg cccgaacgca tcgcgtccat ggtgcagacc ctcaagcggc
     3121 acggcgtcct cgccaaggtg cgcgactcgg cagggcagga gatcgaaggc gcatgcggac
     3181 agttgcgcgc acgggcggcc aaaacgaagt cggacaagac ccgagcatcg gagtagcatg
     3241 gaatcgcagt tcatccctta atcaccataa ggagttagat catgcccaag tcacataatg
     3301 ctcatagaga cagcaagaag aaaccgttgc tgacacccaa ggaaaaacgg atggctaaac
     3361 acgagaagaa acatagccac gacgttcagc cgttcatccc gcctgcgacg actcattaag
     3421 gagttggttc gttgcaaaag ataaagcccg gcatgccggg ctttattgat ttctcctgtc
     3481 ttacattttc gctacttagc ctgatgaaac aacgggtgaa acaactagcc atctcactaa
     3541 ggcagcaaac tgccaagtga ttggttatag ccattcgact aagtcaccaa aaaacggtga
     3601 cataagtcgc cggttatttg tcgaacttca tcacaccgcc cttgcagtcc accctgatgg
     3661 tgtccttcgc ggcaaagcgg ccttccagaa tctgcttcgc gagcgggttc tcc
//
LOCUS       DASUIV010000098         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1139208, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000098 DASUIV010000000
VERSION     DASUIV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139208"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFW59_03615"
     CDS             complement(<1..197)
                     /locus_tag="VFW59_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363333.1"
                     /translation="MIALPQTATTRILLAIALSLLVHAVVLFVPLIALPKSEVPLPPL
                     TAKLEPLPKLAAKPTPPPKKKS"
     gene            complement(194..886)
                     /locus_tag="VFW59_03620"
     CDS             complement(194..886)
                     /locus_tag="VFW59_03620"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5363334.1"
                     /translation="MNWLAHSATWLLCIIGFPALAAPPAGIQATYDIYKDSLLIGRID
                     ESYTRDQDRYTLTSTTTPLGLLAVFKPEKIVMHSNGLVDRHGLRPQLFTHRREASESS
                     AEFNWQTMKLTLSQPPNEFPLQAGTQDRLSAMYQFMFLPLGNVQNVDFAMTNGRKLDS
                     YHYAITRGEPLQTPAGKFDTLYLDSQAKPGESRTEIWLSVQQHYLPCKMIITDADGEH
                     LTQILSKLIIQP"
     gene            complement(889..1527)
                     /gene="purN"
                     /locus_tag="VFW59_03625"
     CDS             complement(889..1527)
                     /gene="purN"
                     /locus_tag="VFW59_03625"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5363335.1"
                     /translation="MKNIVILISGRGSNMQALLKANLPCRIAAVISNRADAEGLSIAR
                     EHGIATAVVAHRDHPDRASFDTALAQAIDDFHPDFVILAGFMRILTEDFVKHYQGRLI
                     NIHPSLLPSYGGLHTHARALHDGVKIHGCTVHFVTPDLDHGPIIIQAAVPVLYNDTEK
                     TLAARVLREEHRIYPQAICWLCNGQIELGQDGRVLFQQRKQTSHALISPGLE"
     gene            complement(1587..>2522)
                     /gene="purM"
                     /locus_tag="VFW59_03630"
     CDS             complement(1587..>2522)
                     /gene="purM"
                     /locus_tag="VFW59_03630"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5363336.1"
                     /translation="EVLTGIGGFGALVEISKKFKEPVLVSGTDGVGTKLKLAFELNRH
                     DTVGIDLVAMSVNDILVQGAEPLFFLDYFACGKLDVDAATEVIKGIAFGCEQSGCALI
                     GGETAEMPGMYPVGEYDLAGFAVGVVEKSSIITGSDIKPGDVVLGMASNGAHSNGYSL
                     VRKIIERSKPDLNAKFDGERTLADCIMAPTRIYVKPLLSLMQSITIKGMAHITGGGIT
                     ENVPRVLPDNVVAAIDGSSWQMPKLFHWLREQGNVNAQEMYRTFNCGIGMVVIVAAED
                     ADRAINELQATGETVSRIGTIRARNGDEHQTQVSE"
BASE COUNT          444 a          718 c          823 g          537 t
ORIGIN      
        1 gacttctttt tcggcggtgg cgtgggtttg gcggcgagct tgggcagcgg ttccagtttg
       61 gcggtgagcg gcggcagcgg cacttcgctt ttcggcaacg cgatcagcgg tacgaacagc
      121 acgacggcat gcaccagcag agacagggcg atggcgagga ggatgcgggt ggtggcggtc
      181 tgcggcagag cgatcacggc tggatgatga gtttgctcag aatctgggtg aggtgctcgc
      241 cgtcggcgtc ggtgatgatc atcttgcagg gcagatagtg ctgttgcacg gacagccaga
      301 tttcggtacg gctctccccc ggcttcgcct ggctatcgag gtacagcgtg tcgaactttc
      361 cggcaggggt ctggagcggt tcgccacggg tgatggcgta atggtagctg tccagcttgc
      421 ggccattggt catcgcaaaa tccacgtttt gcacattgcc gagtggcagg aacatgaact
      481 gatacatcgc actcaggcga tcttgagtgc ctgcctgcaa cgggaattca ttgggcggct
      541 gggacagtgt cagtttcatg gtctgccagt tgaactctgc gctgctctcg ctggcttcgc
      601 gccggtgagt aaatagctgg gggcgcaagc catgccggtc gaccaggccg ttgctgtgca
      661 tgacgatctt ttccggcttg aacaccgcca gcaagccgag cggtgtggtg gtgctggtca
      721 gcgtgtagcg atcctgatcg cgcgtgtaac tctcatcgat ccggccgatc agcaggctgt
      781 ctttgtagat gtcgtaggtg gcctgaatgc cggctggtgg agcggccaat gcagggaaac
      841 cgatgatgca cagcagccag gtggcggaat gcgcgagcca gttcatggct attccaggcc
      901 gggcgagatc agggcgtggc tggtttgttt tcgctgctgg aacaggacac gcccgtcctg
      961 tcccagttcg atctgcccgt tgcacagcca gcagatggct tgcggataga tgcgatgttc
     1021 ctcgcgcaat acgcgcgccg ccagcgtctt ctcggtgtcg ttatataaca ccggcacggc
     1081 agcctgaatg atgatcggcc cgtgatccag atccggcgtg acgaaatgca ccgtgcagcc
     1141 gtgaatcttc acgccgtcgt gcagcgcgcg cgcgtgggtg tgcagcccgc cataagaggg
     1201 tagcagcgaa ggatggatgt tgatcaggcg gccctgatag tgcttcacga aatcctcggt
     1261 gaggatgcgc atgaaaccgg ccagtatcac gaagtcggga tgaaaatcgt cgatggcctg
     1321 cgccagagcc gtatcgaaac tggcgcgatc ggggtggtcg cggtgcgcga ccaccgcagt
     1381 cgcaatgcca tgctcacggg cgatactcag tccctcggca tcagcgcggt tgctgatcac
     1441 cgccgcgatg cggcagggca ggttggcttt cagcagcgcc tgcatattgc tgccgcgccc
     1501 cgagatcagg ataacgatat ttttcatatt cttatctggt gagtagtcag cagtgagcag
     1561 gaaggctgtt cacacttgct gattctctat tcactaacct gcgtctggtg ctcgtcgccg
     1621 ttgcgagccc ggatggtccc gatgcgcgat acggtctcgc ccgtcgcctg caactcgttg
     1681 atggccctgt cggcatcttc ggcggcgacg atcaccacca tgccgatgcc gcagttgaag
     1741 gtgcggtaca tctcctgcgc attgacgttg ccctgctcgc gcagccagtg gaacagcttg
     1801 ggcatctgcc agctgctgcc gtcgatggct gccacgacgt tgtcgggcag cacgcgcggc
     1861 acgttctcgg tgatgccgcc gccggtgatg tgggccatgc ccttgatggt gatgctctgc
     1921 atcagcgaaa gcagcggttt cacgtaaatg cgggtgggcg ccatgatgca gtcggccagc
     1981 gtgcgttcac cgtcgaattt cgcgttcaga tcgggtttgc tgcgttcgat gatcttgcgc
     2041 accagcgaat agccgttcga gtgggcgccg ttggaggcca tacccagcac cacgtcgccg
     2101 ggcttgatgt cgctgccggt gatgatgctg gacttttcca ccacgccgac ggcgaagccg
     2161 gccagatcgt attcgccgac cgggtacatg cccggcattt cggcggtttc gccgccgatc
     2221 agcgcgcagc cggactgttc gcagccgaag gcgatgcctt tgatgacttc ggtggcggca
     2281 tccacatcga gtttgccgca ggcgaagtag tcgaggaaga acagcggctc ggcgccctgc
     2341 accagaatgt cgttgacgct catcgccacc aggtcgattc ccaccgtatc gtggcggttc
     2401 agctcgaacg ccaacttcag cttggtgccg acgccatcgg tgccggagac cagcaccggc
     2461 tccttgaact ttttggaaat ttcgaccagc gcgccaaagc cgccgatgcc cgtgagaact
     2521 tc
//
LOCUS       DASUIV010000099         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1046217, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000099 DASUIV010000000
VERSION     DASUIV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046217"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFW59_03635"
     CDS             complement(<1..1140)
                     /locus_tag="VFW59_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX5363337.1"
                     /translation="MNKNYMNFFLAAIALLGCSAAYAAGGAVADEFKWMTFSVFGVII
                     AITMAITFWAARSTHTTSEFYAAGRSVSGIQNGWAIAGDYLSAASFLGIAGLISLYGY
                     DGFMYSVGWLVAYITVLLVIAEPCRNIGKYTMGDILAFRNDPKKTKVVAALSTITVST
                     FYLTAQMVGGGVLIKTLIGIDYEISVIGVGILMLAYVVFGGMKATTWVQITKAVLLVA
                     ASIILVMMVWAPYGFSLPAYLQAVVGDSDVQKQVAKLLGDKAATMTPEELGQRFLEPG
                     LFLKSPIDQISLGLALVLGTAGMPHILMRFFTVPTAQAARVSVIWAMGIIGGFYVLTL
                     FLGTGAAMLVGADKIASIDAGGNMAGPLLAQFLGGGEQSYMGNFML"
     gene            complement(1137..1460)
                     /locus_tag="VFW59_03640"
     CDS             complement(1137..1460)
                     /locus_tag="VFW59_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX5363338.1"
                     /translation="MSNPDINWAKIDSDPKFQALHRKKTLFLWGLMVFSIIYYFMLPI
                     GAAYYQELFKIKVWGVVNVGILFALSEFVVAWLIAFIYSRKANAQFDSMAQEIIDDVN
                     SGSIK"
     gene            complement(1594..2292)
                     /locus_tag="VFW59_03645"
     CDS             complement(1594..2292)
                     /locus_tag="VFW59_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5363339.1"
                     /translation="MSWLRRWFGRQPDLSEIQQARLDVWRPQAAADVELEGGRFVVVD
                     VETSGLNLAHDRLISIGAVAVVGGRIALGDSFSEVLQQQSASDRQNILLHGITGTAQL
                     DGRPAADVLLDFLEYLGDAPLVAFHVTFDETMIRRALREHLGISFKRPWLDLAYLMPA
                     LRPELARRYRSLDDWGGHFDIRNELRHNALADALATAELFLVAASLARAKGISRFDGL
                     RDLEKAQRWVSGEE"
     gene            complement(2289..>3857)
                     /locus_tag="VFW59_03650"
     CDS             complement(2289..>3857)
                     /locus_tag="VFW59_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX5363340.1"
                     /translation="DTFCYELPAADFHTLLGVSAPFRDFCTRRIANLLEHSKQVIQAQ
                     YTQSGAEQQSLASPLSAIIRRAPVTCSPQTSLREVLQRMHDARIGSMVAVDDAGRPLG
                     ILTLPDVLERIALPQADLAQPVIGVMTTGLTFLSPQALAHEAALLMARQGFRHVLVVD
                     NERLVGLVSEKDLFSLQRVGLRQIGTAIRHAGNVEALQQAALDIRSMVHNMLAQGVAA
                     EQLTQFISAFNDQLTVRVVELECAHGGLDAGVALCWLALGSEGRFEQTLSTDQDNALI
                     FSVPEGMTADQVRAQLLPLAKRINDALAACGFPLCRGNVMASNPQWCLSLDEWKRAFS
                     NWIANGTPEALLHATIFFDFRSLYGAHGLAEELRSWLARAASGNTRFLHQMAENAMSI
                     RPPLGVVRDFVVGKEHTLDLKLNGITPFVDAARIFSLAAGVTHTSTIQRLRASAGRFN
                     IHPSEVEAWVDALLFIQMLRLRHHHDVLQTQGCELDNAIDPEALNELDRRILKEAFRQ
                     ARKLQARLALEYQL"
BASE COUNT          819 a         1261 c         1121 g          656 t
ORIGIN      
        1 cagcatgaag ttacccatgt acgattgctc gccgccgccc aggaactgcg ccagcaacgg
       61 gcctgccatg ttgccgccgg catcgatgga ggcgatcttg tcggcaccga ccagcatcgc
      121 agcaccggta ccgaggaaca gtgtcagcac gtagaagccg ccgatgatgc ccatcgccca
      181 gatcaccgaa acacgcgcag cctgagccgt cggcacggtg aagaagcgca tcaggatatg
      241 cggcatgccg gccgtgccga gcaccagcgc cagacccagc gagatctggt cgatcgggct
      301 cttcaggaac aaacccggct caaggaaacg ctgacccagc tcttccgggg tcatggtcgc
      361 agccttgtcg cccaagagct tggcgacttg cttctgcaca tccgaatcgc cgaccaccgc
      421 ctgcaggtaa gcgggcaggc tgaagccgta aggcgcccac accatcatca cgaggatgat
      481 ggaagcggcc accagcaaca ccgccttggt gatctgcacc caggtggtgg ccttcatgcc
      541 gccgaacacc acgtaggcca gcatcaggat gccgacgccg atcacggaga tttcgtagtc
      601 gatgccgatc agcgtcttga tcagcacgcc gccgccgacc atctgcgcgg tcaggtagaa
      661 ggtggacacg gtgatggtgg acagcgccgc aacgaccttg gtcttcttcg ggtcgttacg
      721 gaaggcgagg atgtcgccca tggtgtactt gccgatgttg cggcagggct cggcgatcac
      781 cagcagcacg gtgatgtaag ccaccagcca gcccaccgaa tacatgaagc catcgtaacc
      841 gtacagcgag atcagacccg cgatacccag gaaggatgcg gcggacagat agtcgcccgc
      901 gatcgcccag ccgttctgga tgccgctgac cgatcggccc gccgcataga attcggaagt
      961 ggtatgggtg gaacgcgctg cccagaaggt gatcgccatg gtgatggcga tgatcacgcc
     1021 gaacacgctg aaggtcatcc acttgaactc gtcggcaacc gcaccgcctg ccgcataggc
     1081 ggcactacag cccagcagcg cgatggccgc caggaaaaaa ttcatataat ttttgttcac
     1141 ttgatgctcc cgctatttac gtcatcgatg atttcctgcg ccatcgaatc gaactgggca
     1201 ttagccttgc gcgaatagat gaaggcgatc agccatgcca ccacgaactc ggacagcgcg
     1261 aacaggatgc cgacgttgac cacgccccac accttgatct tgaacagctc ctgataatag
     1321 gccgcaccga tcggcagcat gaaatagtag atgatcgaaa agaccatcaa cccccacagg
     1381 aacaacgtct tcttgcgatg caaggcttga aacttcgggt cggaatcgat cttcgcccaa
     1441 ttgatatctg gatttgacat catcactcca aaaaaacaac cataaaaaac cgcgaatgga
     1501 tcacggcgca cctgaacagc aggcgggaag gttacgtgaa gtgctcaggc aacgcaattt
     1561 ttcatgcctt ttccattatt aagcaagtgg ttatcactcc tccccgctca cccagcgctg
     1621 cgccttttcc agatcgcgca ggccgtcgaa acgggagatt cctttggccc gcgccagtga
     1681 tgcggcgacc agaaacagct cggcggtggc cagtgcatcg gccaatgcgt tgtggcgcag
     1741 ctcgttgcgt atatcgaaat gtccgcccca gtcgtccagc gaccggtaac ggcgcgccag
     1801 ttcgggacgc agtgcgggca tgagataggc cagatcgagc caaggacgct taaaagagat
     1861 tcccagatgt tcgcgcaacg cgcgcctgat catggtctcg tcgaaggtga catgaaacgc
     1921 gaccagcggc gcatcgccca gatactcgag aaagtccagc agcacatccg cagcaggccg
     1981 cccgtccagc tgggccgtgc ccgtgatgcc atgcagcagg atgttctgcc tgtcgctggc
     2041 cgactgttgc tgcaacacct ccgagaaact gtcgcccagc gcgatgcgac cgccgaccac
     2101 cgccaccgcg ccgatcgaga tcagcctgtc gtgcgccaga ttcaagccgc tggtctccac
     2161 atccaccacg acgaaacgcc ctccctccag ctccacatcc gccgcagcct gcggccgcca
     2221 gacatccaga cgcgcctgct gaatctccga caaatccggc tgcctgccga accagcgacg
     2281 cagccagctc acagctgata ttccagcgcc agcctggcct gcagcttgcg cgcctgacgg
     2341 aacgcctctt tcaggatgcg cctgtccagc tcgttcagcg cctccggatc gatcgcgtta
     2401 tccagctcgc atccctgtgt ctgcagcaca tcgtgatgat gacgcaggcg cagcatctgg
     2461 atgaacagca gggcatccac ccaggcttcg acttccgagg gatggatatt gaaccggccg
     2521 gcactcgccc tcagccgctg tatcgtgctg gtgtgcgtca cgccggcggc cagactgaag
     2581 atgcgcgccg catccacgaa gggcgtgatg ccgttgagct tgagatccag cgtgtgctct
     2641 ttgcccacca cgaagtcgcg caccacgccg agcggggggc ggatgctcat cgcgttttcc
     2701 gccatctgat gcaggaagcg ggtattgccg ctggcagccc gggccagcca tgagcgcaac
     2761 tcctcggcca gaccgtgcgc gccatacagg gaacggaaat cgaagaatat cgtggcatgc
     2821 aacagcgcct ccggcgtgcc gttggcgatc cagttggaga aggcgcgctt ccactcgtcc
     2881 agcgacaggc accactgcgg attcgaggcc atcacattgc cgcgacacag cgggaaaccg
     2941 caggcggcca gtgcgtcgtt gatgcgtttg gcgagcggca gcaattgcgc gcgcacctga
     3001 tcggccgtca tgccttcggg tacgctgaat atcagcgcat tgtcctgatc ggtgctcagc
     3061 gtctgttcga aacgcccctc cgaacccagc gccagccagc acaaggccac ccccgcatcg
     3121 agaccgccat gcgcgcactc cagctcgacc acccgcaccg tgagctggtc gttgaaggct
     3181 gagatgaact gggtcaactg ctccgccgcc acgccctgcg ccagcatgtt gtgcaccatg
     3241 ctgcggatat ccagtgccgc ctgttgcaac gcttcgacat tgccagcatg gcggatggcc
     3301 gtgccgatct ggcgcaagcc cacgcgttgc aggctgaaca gatctttttc cgaaaccagc
     3361 ccgaccagac gctcgttatc gaccaccagc acatgccgga agccctgcct ggccatcagc
     3421 agggcggcct catgcgccag tgcctgcggc gacagaaagg tcaggccggt cgtcatcacg
     3481 ccgatcaccg gctgcgccag atcggcctgc ggcaacgcaa tgcgctccag cacatcgggc
     3541 agcgtcagaa taccgagcgg ccgcccggcg tcatcgaccg ccaccatcga accgatgcgc
     3601 gcgtcatgca tccgctgcaa cacttcgcgc aggctggttt gcggcgaaca ggtgacgggc
     3661 gcgcggcgga tgatggccga cagcgggctg gccagcgact gctgctcggc tcccgactgg
     3721 gtgtactgcg cctgaatgac ctgcttggag tgttcgagca gattggcgat acggcgcgtg
     3781 cagaaatcgc ggaacggcgc gctgaccccg agcagcgtat gaaaatccgc cgccggcaat
     3841 tcgtagcaga acgtatc
//
LOCUS       DASUIV010000100         6606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_767794, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000100 DASUIV010000000
VERSION     DASUIV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767794"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFW59_03655"
     CDS             <1..329
                     /locus_tag="VFW59_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5363341.1"
                     /translation="HLDLMDLLAMYTGRANAPLDELARLIGFPGKLGMDGSKVWEAYQ
                     QGRIAEIRNYCETDVVNTWLVFARFQLMRGQFSLARYEQELELVRSTLGKSAEPHWAE
                     YLAAWR"
     gene            333..710
                     /locus_tag="VFW59_03660"
     CDS             333..710
                     /locus_tag="VFW59_03660"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5363342.1"
                     /translation="MHKLRRAWLILGWFWIAAVFYISLMPNPPEPVRFPNIDKLEHAF
                     TYCLLMLWFSQVYVRRLRRLFAAAWLVAMGVAIEILQDMTGYRLFEYGDMLANATGVV
                     LGWVWARTALGQVGRTLETKFLK"
     gene            721..2088
                     /gene="rlmD"
                     /locus_tag="VFW59_03665"
     CDS             721..2088
                     /gene="rlmD"
                     /locus_tag="VFW59_03665"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX5363343.1"
                     /translation="MTETINTPPVVAIESLDQEGRGIAHHEGKVIFIEGALTGERVTY
                     NTYRKKPSFEMAHLSSIVKSSFMRVQPKCIHFGVCGGCSMQHLETRAQVAVKQRILED
                     NLARIGKVKPESILPPIHGLPWGYRQRARLSVRHVIKKNKTMVGFHERSSRYVADMSS
                     CEILPPKISKLIPLLADLIGGLSIRDHLPQIEVACGDKVDALVLRIMQPLSASDEAAL
                     RAFADAHAVQFWLQTKGPETVAPFYPLDAPALTYTLPEYNVEMPFAPTEFTQVNHQLN
                     RVMVHRAIRLLDPQPNERIADFFCGLGNFTLPIARSGARVLGIEGSLALVKRAGQNAA
                     HNGLAKDSPRPLAGEGVGERGYAEFQTMNLFEMDEAELARRGHFDKWLIDPPRDGAME
                     LIKAITPQTAPLRIVYVSCNPATLARDAEILVKTHGYVLKAAGVMNMFPQTSHVESIA
                     VFDWE"
     gene            2172..2642
                     /locus_tag="VFW59_03670"
     CDS             2172..2642
                     /locus_tag="VFW59_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5363344.1"
                     /translation="MKYDDLVAEALARVKEIMPWDLSRMLAAGNKPLLLDVREPSEFA
                     MLRIPGSINAPRGVLEQSCEWDYDETLPELASGREREIVVICRSGKRSVLAADTLQRM
                     GFVNVVSLKTGVRGWNDFEQPMEDAAGNPIDADAGDVLLAPRVTPEQRKPVFLP"
     gene            3111..4745
                     /locus_tag="VFW59_03675"
     CDS             3111..4745
                     /locus_tag="VFW59_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5363345.1"
                     /translation="MFKNMKIGVRLGLGFGLVLLLLAIVTFIGVTRLSDLNDGMDKLA
                     NDRWPKVEKMQQMRDLTNDNARAIRNMMLTDDLQETRKQYERLKGNIVAIEKLHEELK
                     VVITSEEGKRLIAGLERETDKYSADMEKAAQLALQNKNVEAAAVLFGDLRKSQKVYFA
                     ALDELNSFQAKQIVSAANEAKASYVAARNMMVALGIGAALLAIGIAFWVTRSITRPIN
                     EAVGVADALAAGDLTVKIEVDSTDETGMLLNAMQTMVGKLSQIISEVRGAAENLTSAA
                     EEVSSTSQSMSQATNEQAASVEETSASVEQMGASINQNSENAKVTEGMATQAAKQATD
                     GGVAVKETVTAMKQIAGKIGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVR
                     KLAERSQVAAQEIGELASGSVDKAEDAGKLLDQIVPAINKTSDLVQEITAASEEQSSG
                     ATQINTAMNQLNQITQQNASATEELAATAEEMSSQAEQLQQLMSFFRVSGAAAPVVKS
                     SVHTPTVSAPAAKSGKAAKKLAAAPVSHNEAEFVKF"
     gene            complement(4944..>6606)
                     /locus_tag="VFW59_03680"
     CDS             complement(4944..>6606)
                     /locus_tag="VFW59_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5363346.1"
                     /translation="NKIAAGDLSANIQVDSKDETGQLLVAMKNMNDTLKGLMTDTDVL
                     VKAAAVGDLNTRADASKYQGDFRKLVQGVNDTVTNIAEPMKVTSDYIDQIARGVIPAQ
                     ITTAYQGEYLVIRNNLNGMIKMMGDLLSETDILIQGAAAGELDRRANAAQFQGGWNQL
                     VVGVNQIVDAVDIAFKDTVRVAEALAKGDLSQKVTREYRGTFNVVKQSVNGTADALTL
                     IVGEIRDIVASANKGDFNSKMNLDGKLGFAKELSELLNQLSDTVDIAFKDTIHVAGAL
                     ERGDLTQVVTRDYQGAFDQVKQSLNNTVGKLSQVIGEVNAAATNIASASEEVSSTAQS
                     MSQATNEQAASVEETSASVEQMGASINQNTENARVTDGMAGQASKQANEGGVAVKETV
                     SAMKQIAGKIGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLAERSQVA
                     AQEIGELASGSVSKAEDAGRLLDQIVPAINKTSELVQEITAASEEQSSGATQINTAMN
                     QLNQITQQNASSTEELAATAEEMSSQAEQLQQLMSFFRVSGSARA"
BASE COUNT         1340 a         1854 c         2007 g         1405 t
ORIGIN      
        1 gccatctcga tctgatggat ctgctcgcga tgtacaccgg ccgggccaac gcgccgctgg
       61 atgaactggc cagactgatc ggcttccccg gcaagctggg catggacggc agcaaggtgt
      121 gggaggcgta tcagcagggc aggatcgccg agatacgcaa ctactgcgag accgatgtgg
      181 tgaacacctg gttggtgttt gctcgcttcc agttgatgcg cgggcagttc agccttgcgc
      241 gctacgagca ggaactggag ctggtgcgca gcacgctggg caagtccgcc gagccgcact
      301 gggccgaata tctcgcggcc tggaggtaaa ccatgcacaa attacgccgt gcctggctga
      361 tactggggtg gttctggata gcggccgttt tttatatttc gctgatgccg aatccgccgg
      421 agccggtacg cttcccgaat atcgacaagc tggagcatgc gttcacctac tgcctgctga
      481 tgttgtggtt ctctcaggtg tatgtgcgcc gtctgcggcg cctgttcgcg gcggcatggc
      541 tggttgcgat gggtgtggcg atagagatac tgcaagacat gaccggttac cgtctgttcg
      601 agtatggcga catgctggcg aatgccaccg gtgtggtgct gggctgggtg tgggcgcgca
      661 ctgcgctcgg gcaggttggg cgtacgctgg aaacaaaatt tttgaaatag ccggacaatt
      721 atgactgaaa caatcaatac gccacccgtg gtggcaatcg agtcgctcga tcaggaaggg
      781 cgcggcatcg cgcatcatga aggcaaggtg atctttatcg agggcgcatt gaccggcgag
      841 cgcgtcacct ataacaccta tcgcaagaag cccagcttcg agatggcgca tctgagcagc
      901 atcgtcaagt cgtcgttcat gcgcgtgcag cccaagtgca tccacttcgg cgtgtgcggc
      961 ggctgttcca tgcagcatct ggagacgcgc gcgcaggtcg cggtaaagca gcgcatcctc
     1021 gaagacaatc tggcgcgcat cggcaaggtc aagcccgaga gcatcctgcc gccgatccac
     1081 ggcctgcctt gggggtatcg ccagcgcgcg cgtctgtcgg tgcgccatgt gatcaagaag
     1141 aacaagacca tggtgggctt ccatgagcgc tccagccgct atgtggccga catgtcgagc
     1201 tgcgagattc ttccgccaaa aatctccaag ttgatcccgc tgctcgccga cctgatcggc
     1261 ggactgtcca tacgcgatca tctgccgcag atcgaagtgg cctgtggcga caaggtggac
     1321 gcgctggtgc tgcgcatcat gcagccgctc agtgcgagcg acgaggctgc cttgcgcgct
     1381 tttgccgatg cgcatgccgt gcagttctgg ctgcagacca aggggccgga gacagtggcg
     1441 ccgttctatc cgctggatgc gccggcgctc acttacaccc tgcccgagta caacgtcgag
     1501 atgccgttcg caccgaccga gtttacccag gtgaatcatc agctcaaccg cgtgatggtg
     1561 catcgcgcca tccgtctgct cgatccgcag ccgaacgaac gcatcgccga tttcttctgc
     1621 ggactgggca acttcacgct gcccatcgcg cgcagtggcg ctcgggtgct gggtatcgaa
     1681 ggcagcctgg cactggtgaa acgcgccggt cagaacgccg cgcacaacgg cctcgctaag
     1741 gactcccctc gcccgcttgc gggagagggg gtgggggaga gggggtatgc cgaatttcag
     1801 accatgaacc tgttcgagat ggatgaggcc gaacttgccc ggcgcggcca cttcgacaag
     1861 tggctgatcg atccgccgcg cgacggtgcg atggaactga tcaaggccat cacgccgcag
     1921 accgcaccgc tgcgcatcgt ctatgtgagt tgcaaccccg ccacgctggc gcgcgatgcc
     1981 gagatattgg tgaagacgca cggatatgtg ctgaaggcgg cgggcgtgat gaacatgttt
     2041 ccgcagacct cgcatgtgga atccatcgcg gtgttcgact gggagtagca ggcggatgca
     2101 agtcttgagg tgttacggcg cgatcgagcg aagccgactc caaggccgaa tgaaattttt
     2161 ggagtgaaat aatgaaatat gacgatctgg tcgccgaggc actcgcgcgg gtgaaggaga
     2221 tcatgccgtg ggatctgagc cgtatgctgg ccgccggaaa caagccgctg ctgctcgatg
     2281 tgcgcgagcc gtccgagttc gcgatgctgc gcatccccgg ttcgatcaac gcgccgcgcg
     2341 gtgtgctgga gcagtcctgc gagtgggatt acgacgagac cttgccggaa ctggcgtcag
     2401 ggcgcgagcg cgagatcgtg gtgatctgcc gttccggaaa acgatcggtg ctggccgccg
     2461 acaccctgca gcgcatggga ttcgtcaacg tggtctcgct caagaccggc gtgcgtggct
     2521 ggaacgactt cgaacagccg atggaagatg cggcaggcaa tcccatcgac gcggatgcgg
     2581 gcgacgtgtt gctcgcacca cgcgttacgc cggaacagcg caagccggtt ttcttgccgt
     2641 aaaagtccag ttgtactaaa tgcgtgctca atctggagca tgtaacaaaa tccggcagac
     2701 acaacagtcg ttgttcagcg ttgcaacctg caggatatgc cggaagcggt ttgctttctg
     2761 cttgaaccca tactctccct tcctgttggc gcttccgttc tacaaaacca tttgtcggat
     2821 agtgcaattt tccctacaaa aaaagcagaa tcgtctgaca tttatttcca cagcctttcg
     2881 acatgattcc aatcgcaact catggggctg tgtgaacagg caggcgaacg gcaatttttt
     2941 tacctataaa tataagcatt tcgtaattta attaaattgt gtttgtttgg tatggggcgt
     3001 atcgcttggt tctgatttga tgcagatttt gagaacaaca taatttgtcg ttttgctaaa
     3061 ggaagcggct ggttgcgtgt cgaatttttt atgaaaaaat ggagcaggga atgttcaaga
     3121 atatgaagat tggtgtgcgt ctaggattgg gtttcggttt ggtgctgtta ttgctggcga
     3181 tcgttacgtt catcggcgtc acacgcctgt cggacctcaa cgatggaatg gataaactgg
     3241 cgaatgatcg ctggccaaaa gtagagaaga tgcagcagat gcgcgatctg accaacgaca
     3301 atgcgcgcgc tatccgcaac atgatgctga ccgatgattt gcaggaaacc cgcaagcagt
     3361 atgaaagact taaagggaat atcgtggcta tcgagaagct gcatgaagaa ctcaaggtgg
     3421 tcatcacaag cgaggagggg aagcgcctga ttgcaggcct ggagcgggag acggacaaat
     3481 attcggcaga tatggaaaaa gccgcccagc tcgcactaca gaacaagaat gtcgaggcgg
     3541 cagccgtttt gttcggcgac ctgcgcaaat cgcagaaagt ctattttgct gcgctcgacg
     3601 aattgaacag ctttcaggcc aaacagattg tgtctgctgc caacgaggcg aaagcttcct
     3661 acgtcgccgc ccgcaacatg atggtggcac tgggtatcgg agctgcgtta ctcgctattg
     3721 gcatcgcatt ctgggtcacc cgttctatca cgcgtcctat caatgaggca gtcggcgtcg
     3781 cggatgcgct ggcggcaggc gatctgacgg tcaagatcga ggtggattcc acggatgaga
     3841 ccggcatgtt gctgaacgcg atgcagacga tggtcggcaa actgtcgcaa atcatcagcg
     3901 aggtgcgcgg tgcggctgag aatctgacca gtgcggcgga agaagtctcc agcacttcgc
     3961 aaagcatgtc gcaggcgacc aatgaacagg ctgcttcggt tgaggagact tccgcctcgg
     4021 tcgagcagat gggcgcgtcc atcaaccaga actccgaaaa tgccaaggtc accgaaggca
     4081 tggcgacaca ggccgccaag caggcgaccg acggcggtgt ggcagtgaag gaaaccgtga
     4141 ccgcgatgaa acagatcgcc ggcaagatcg gcatcatcga cgatatcgcc taccagacca
     4201 acctgctggc cttgaacgcg gcgattgaag cggcgcgagc tggcgaacac ggcaagggct
     4261 ttgcggtggt ggcggcggaa gtgcgcaagc tggccgaacg ctctcaggta gccgcgcagg
     4321 aaatcggcga actggcgagc ggttcggtgg ataaagcaga agatgccggc aagctgctgg
     4381 accagatcgt gcctgcaatc aacaagacct ccgacctggt gcaggaaatc accgcagcca
     4441 gcgaggagca atcctcgggc gctacgcaga ttaacaccgc gatgaaccag ctcaaccaga
     4501 tcacccagca gaacgccagt gcgactgaag agttggccgc gactgcagaa gagatgtctt
     4561 ctcaggccga gcagttgcag caactgatga gcttcttccg tgtgagcggt gcggccgcgc
     4621 cggttgtgaa gagcagcgtt catacgccca ccgttagcgc gcctgctgcc aaatcaggca
     4681 aggccgcgaa aaaactcgct gccgcgcctg tctcgcataa cgaggcggag tttgtgaagt
     4741 tctaagcggt tttcgcagga gcgacttcat aaccagcgac ttggctggcg ttgtttgcca
     4801 gcttagtcga atggctagta gtttcatcag ggggatttaa ccaacttgag caacggcctc
     4861 attgccacaa atccccccta gccccccttt cgacaaaggg gggaactagc cgattacgtc
     4921 gaagggccgc tcctgcgaaa accttacgct ctcgccgaac cgctcacccg gaagaagctc
     4981 atcagctgct gcagctgctc ggcctgagac gacatctctt ctgcggtcgc cgcgagttct
     5041 tcggtcgagc tggcattctg ctgggtgatc tggttgagct ggttcatcgc cgtgttgatc
     5101 tgcgttgcac ccgagctttg ctcctcgctg gctgcagtga tttcctgcac cagttcggag
     5161 gtcttgttga tcgccgggac gatctggtcg agcagcctgc cggcatcttc cgccttgctc
     5221 acgctgccgc tggccagctc gccgatctcc tgtgcggcaa cctgagaacg ttccgccagc
     5281 ttgcgcactt cggccgccac caccgcgaag cccttgccgt gttcgcccgc acgcgccgct
     5341 tcgatcgccg cgttcagtgc cagcaggttg gtctggtaag cgatgtcgtc gatgatgcca
     5401 atcttgcccg cgatctgttt catcgcgctg acggtttctt tgaccgccac gccaccttcg
     5461 ttcgcctgct tggaggcttg tccggccatg ccgtcggtca ccctcgcatt ctcggtgttc
     5521 tggttgatcg aagcacccat ctgttcgacc gaggcggagg tctcttcaac agaagcggcc
     5581 tgctcgttgg tcgcctgcga catgctctgc gcggtgctcg atacttcttc cgaagccgag
     5641 gcgatgttgg ttgctgccgc gttcacttca ccgatcacct gggagagttt gccaacggtg
     5701 ttgttgaggc tttgtttgac ttggtcgaac gcgccctggt aatcgcgggt cacaacctgg
     5761 gtcagatcgc cgcgttccag tgcgccggca acatggatgg tgtctttgaa tgcgatatcc
     5821 acggtgtcgg acaactggtt cagcaactcg gaaagctctt tggcgaaacc taacttgccg
     5881 tccagattca tcttggaatt gaaatcgcct ttattcgccg aagccacgat gtcgcggatt
     5941 tcgccgacga tcagggtcag cgcatcggct gtgccattca cgctttgctt gacgacattg
     6001 aacgtaccac gatattcgcg ggtaactttc tggctcaaat ctcccttcgc caacgcttcg
     6061 gcaacccgga cggtgtcctt gaacgcgatg tctacggcat caacgatctg gttcacgccg
     6121 accaccagct ggttccagcc gccttggaac tgggcggcat tggcgcgtct gtccagctcg
     6181 cctgccgccg ctccctgaat caggatgtcg gtctcggaca gcagatcgcc catcatcttg
     6241 atcatgccgt tgaggttgtt gcggatcacc aggtactcgc cctgataggc ggtggtgatc
     6301 tgcgcgggga tcacgccacg ggcgatctgg tcgatgtagt cggaggtgac cttcatcggc
     6361 tcggcgatgt tggtcacggt gtcgttcaca ccctgcacca gcttgcggaa gtcgccctga
     6421 tacttgctgg cgtcggcacg ggtgttcaga tcgcccaccg cagcggcctt gaccagcacg
     6481 tcggtgtcgg tcatcaagcc tttcagcgtg tcgttcatgt tcttcatcgc caccagcaac
     6541 tgtccggtct cgtccttgct gtcgacctga atattggccg acagatcgcc cgccgcaatt
     6601 ttattg
//
LOCUS       DASUIV010000101         9145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1139969, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000101 DASUIV010000000
VERSION     DASUIV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139969"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1019
                     /gene="hypE"
                     /locus_tag="VFW59_03685"
     CDS             3..1019
                     /gene="hypE"
                     /locus_tag="VFW59_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX5363347.1"
                     /translation="MDWKTGRVDMSYGSGGRAMAQLISELFAAEFDNPYLAQGNDGAL
                     LPPANGRLVMSCDSHVVSPLFFAGGDIGCLSVHGTINDVAMMGARPLYLAASFILEEG
                     FPLSDLKRIVVSMAQAAREAGVPIVTGDTKVVEQGKGDGVFITTTGVGVARDGITLSG
                     DLAKPGDRILLSGAIGDHGMAVLSQREGLTFDAPIVSDTAALHGLVAAMLDCGADLHV
                     LRDPTRGGVATTLNEIATQSGVGMLLQENAIAVNQPVAAACEFLGLDPLYVANEGKLI
                     AICAAQDAQRLLATMRAHPLGAESSIIGEVIEDAHGFVQMTTRLGGRRIVDWLAGEQL
                     PRIC"
     gene            1020..2870
                     /gene="uvrC"
                     /locus_tag="VFW59_03690"
     CDS             1020..2870
                     /gene="uvrC"
                     /locus_tag="VFW59_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX5363348.1"
                     /translation="MTEAPNDPKPAEAKPGNNQFDPKPVLDSLPLLPGVYRYYDSKGE
                     VLYVGKAKQLKKRVSSYFQKTNISPRIRLMVSHIARIETTVTRSEAEALLLENNLIKS
                     LRPRYNILFRDDKSYPYIVLTGDQFPRLTYYRGVPNRQHQHFGPYPNSHAAKESIQLL
                     QKIFRIRTCENSVFNNRTRPCLLHQIHRCTAPCVNLISDEDYRADIRNAVLLLQGKHQ
                     EVEQTLSAAMQQAAENLHFEQAAALRDQLRALHTVQQKQFVESTGSTTDADIIALAQL
                     DGMVCLNLAMVRGGRHLGDKSFFPDHAEDLSAEEIMQAFIAQHYLERSVPPLLVLNEE
                     CHDETLAQLLSEQAGRAVKISQAASGERKQWLEMGQRNAMLALEQRKAQQGGQQRRLD
                     KLREWLDMPALQRIECFDISHTMGEATQASCVVYENLDMRSAQYRRYNITGITAGDDY
                     AAMRQALTRRYQKLSSGEGARPDLVLIDGGLGQLHIAMEVMQELGLNDLHLVGVAKGV
                     ERKAGMEQLIFTDGSSRQMRSDDPALHLIQQIRDEAHRFAITGHRAKRGKARTASSLE
                     EISGVGDKRRRSLLTHFGGLREVAQASVEQLSQVEGISTALAEKIYQQLH"
     gene            2962..3534
                     /gene="pgsA"
                     /locus_tag="VFW59_03695"
     CDS             2962..3534
                     /gene="pgsA"
                     /locus_tag="VFW59_03695"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX5363349.1"
                     /translation="MFNNIPNLLTWLRILLIPVFVAVFYLSDTTLAPHLKNLISTSVF
                     LLAAVTDWLDGYLARRLNQSSAFGAFLDPVADKLMVAAALIVLVKLGRADAIIAFIII
                     GREITISALREWMAKLGESKNVAVSMLGKIKTTFQMIAILLLLYYDRLLGIDCQAIGS
                     VLLYLAALLTLWSMGYYLVLASPQLKKSRN"
     gene            3574..3650
                     /locus_tag="VFW59_03700"
     tRNA            3574..3650
                     /locus_tag="VFW59_03700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3608..3610,aa:Pro,seq:tgg)
     gene            3692..3768
                     /locus_tag="VFW59_03705"
     tRNA            3692..3768
                     /locus_tag="VFW59_03705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3726..3728,aa:Arg,seq:tct)
     gene            3892..3967
                     /locus_tag="VFW59_03710"
     tRNA            3892..3967
                     /locus_tag="VFW59_03710"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3925..3927,aa:His,seq:gtg)
     gene            4085..6046
                     /locus_tag="VFW59_03715"
     CDS             4085..6046
                     /locus_tag="VFW59_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5363350.1"
                     /translation="MMVKFRYPPLWVAMNAVLLTSSVWAASEPSEQDYFQEFPVVLSA
                     SRLTQPQSEAPNAMTVIDRKMIIASGFRNIVDLFRLVPGMYVGYDSGNAPFVSYHGAT
                     DQYSRRMQVLVDGRTVYLPPFGGVDWLDLPLHIDDIERIEVIRGPAAASHGANSLQGV
                     ISIITRDAASASGASVSLTKGDGGIADVAARLSSHGDNLDYRVTLTNRSDNGYDLPVL
                     NDGNNTSQINLRSNYRFNERNNVDFQFGYSEGIRGAGNSQRLPNEPFRNINTSSNFQQ
                     VDWLHALQGGDELKLNYYHIARDYKDNSPVAFEKDNYQVHRHELGLQHTLSLGDSNRV
                     VWGGAVRYDTAVSSLYFKEPQSQRLTQLFAHDELRVTSSLLANLGAMFEDDGIGHKNL
                     SPRASLNYHITPEHTLRAGASVAYRNPVMFEQKANTSYLGGRQYASAGVQRPEKVISK
                     EIGYLGELKEIGVSLDARIYQDRVSDYIFFDPLTPALFSFSNLYAANYQGFESTLKYR
                     WRQQSNVIFNYSHQRASCTATGSLTMPAMVPVLQAFMNQCPLVVPADSGSILLTEQLT
                     PDLQISAGYYHQEPVQVFDAIWSQKLMRRVDMRMAYAFGQRGAPGGGEVALVVQNAFQ
                     DNYTEYTNVPQKAGFVFNRRAYLTATLAF"
     gene            6057..6965
                     /locus_tag="VFW59_03720"
     CDS             6057..6965
                     /locus_tag="VFW59_03720"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX5363351.1"
                     /translation="MRRSLALLILLAAFFMLPVAAWAGDLHVLLILSDNTPPYQSFAK
                     KLKDSLPSSIQLTIQEAGKPAVASSDLVVTVGMKAAETAISNPRGPILAVMIPKIGYD
                     NLIVARSIPQDISAIYLDQPWGRQLKFLSAVMPNLNKVGLIYSPENHPEISTLLNTAT
                     SHGSTVVAQMVRSEDGLFASLEKVLSDSDVLLAIPDGRIYNASNIRNILLTSYRHKVP
                     LIGFSQGYVNAGSLAAIFTPPEQVAVQTARQLIAFEQKRVLAAPRYPDQFTIAVNLQV
                     ARSLGLTLPSEDLIRKRMSDSGEGSR"
     gene            6962..8929
                     /locus_tag="VFW59_03725"
     CDS             6962..8929
                     /locus_tag="VFW59_03725"
                     /inference="COORDINATES: protein motif:HMM:NF021499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5363352.1"
                     /translation="MKRFGISTQITALTLIPLLVVVISMEAFFLRARFADIDADLLGH
                     GKLMAHQLAGSAEYGVFSNNLAFLASISENALLQREVRSITIMDGNDKVLFSSRGARH
                     RDLDNKAEGNSTEQNISPAQIGLLNPVKTDAKSVWIYQPIVPTQVNLDDMEGMHSAQQ
                     IGAVIVEMSRADSDHRKSEMLWFNIAVTALILAFPLYAIFLASRSITHPIRRLSNAVL
                     KIAQGHLETRVDASRNQTIELASLSSGINEMAAQLQAERAILEQRIEEATLALREKKE
                     EAERASHDKSHFLAVASHDLRQPLHALGLYIAELKRNSTGDEQQHLIKQIERSAESLS
                     TLLNALLDISKLDAGAIVPQIQSCDLNFMLSQVAADYQMLSQIRNIRLVVRACPIHVS
                     SDPMLLERILMNLVSNALRYTPQNGCVMIACRHRNNHLRIEVRDNGIGITASDQANIF
                     REFFQIAQPQLDSSKGLGLGLAIVDRLAKLLGHKIELRSAPAKGTVFAVEVPIAATLA
                     GEASFISSGRDLEPEGLIEHSPLTGKRLLVVDDDDMVLNSTASILTSWGCKVTLATSL
                     PEVERHLQDGMHWDMIISDYQLENHTTGIDVIDQVRRHLAKELPCILISGDTGPAVLK
                     LAAVGGYHLLHKPVKPAKLRSLVLHLLDEEK"
     gene            complement(8897..>9145)
                     /locus_tag="VFW59_03730"
     CDS             complement(8897..>9145)
                     /locus_tag="VFW59_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363353.1"
                     /translation="FWKTYIESRGITAVIAMTALACTIAIAPKPAEAAILHLGNYGEE
                     RHSHDLESRGIYIMRISEGGSLERFLARLLFLIQQMKN"
BASE COUNT         2078 a         2432 c         2572 g         2063 t
ORIGIN      
        1 cgctggactg gaagaccggg cgtgtggaca tgtcttatgg ttccggcgga cgcgcgatgg
       61 cgcagttgat ctcggaactg tttgctgcgg aattcgataa tccctatctg gcacagggca
      121 atgacggcgc attgctgcct ccggccaatg gacggctggt gatgtcctgc gacagccatg
      181 tggtgtcgcc gctgttcttc gcgggcggcg atatcggctg tctgtcggtg cacggcacga
      241 tcaacgatgt cgcgatgatg ggcgcacgac cactttatct ggccgcatcg ttcattctcg
      301 aagaaggctt cccgctgagc gatctgaaac gcatcgtggt atccatggcg caagcagcgc
      361 gtgaggcggg ggtgcccatc gtcaccggcg acaccaaggt ggtggagcag ggcaagggcg
      421 acggtgtgtt catcaccacg actggcgtcg gggtggcgcg cgacggcatc accctgtccg
      481 gcgatctggc gaagccggga gaccggatac tgctttccgg cgcaatcgga gatcacggca
      541 tggcggtgtt gtcgcaacgc gaggggctga ctttcgatgc gcccatcgtg tcggatactg
      601 ccgcgttgca cgggctggtc gcggcgatgc tggactgcgg ggcggatctg catgtgctgc
      661 gcgacccgac gcggggcgga gttgccacga cgctgaacga gatcgccaca caatccggcg
      721 tgggcatgct gttgcaggag aacgcgattg cggtgaacca gccggttgcc gcagcctgcg
      781 agttcctcgg gctggatccg ctgtatgtcg cgaacgaggg taaactgatc gccatctgtg
      841 cggcgcagga tgcgcaacgc ctgctggcca cgatgcgcgc tcatccgctg ggagccgaat
      901 cttccatcat cggcgaggtg atcgaagacg cccacggatt cgtgcagatg accaccaggc
      961 tgggcggtcg tcgcatcgtc gattggctgg caggagagca attgccgagg atatgttgag
     1021 tgactgaagc accgaatgac cctaagcccg cagaggcaaa acccggcaac aatcaattcg
     1081 accccaagcc ggtactggat agtttgccgc tgttgccggg ggtatatcgc tattacgaca
     1141 gcaagggaga agtgctgtat gtcggcaagg ccaagcagct gaagaagcgc gtctcgtcct
     1201 attttcagaa gaccaatatt tcgccgcgta tccgcctgat ggtgtcgcat atcgcgcgca
     1261 tcgaaaccac cgtcacacgc tccgaagccg aggcgttgtt gctggaaaac aacctgataa
     1321 agtctttaag accccgatat aacatactgt ttcgggatga caagtcttat ccgtatattg
     1381 tgctgaccgg agatcaattt ccgcgcctga cctactatcg cggcgtgccc aatcgccagc
     1441 atcagcactt cgggccatac ccgaattcac atgcggcgaa ggaaagcatc cagctgctgc
     1501 agaagatatt ccgcatccgc acctgcgaga acagcgtgtt caacaatcgc acgcgcccct
     1561 gcctgttgca tcagatccat cgctgcaccg ccccctgtgt caacctgatt tcggacgaag
     1621 attaccgggc cgatatccgc aatgcggtgc tgttgctgca agggaaacac caggaggtgg
     1681 aacagacgct gagtgcggcg atgcagcagg cggcggagaa tctgcacttc gagcaggccg
     1741 ccgccttgcg cgaccagttg cgcgcgctgc acaccgtgca acagaaacag ttcgtcgaat
     1801 ccaccggcag cacgaccgat gccgacatca tcgcgctggc acaactggac gggatggtgt
     1861 gcctgaatct ggcgatggtg cgcggcgggc gtcatctggg cgacaagagc ttcttcccgg
     1921 atcacgccga ggatctgagt gcggaagaga tcatgcaggc ctttatcgcc cagcattatc
     1981 tggagcgcag cgtgccgccg ttgctggtac tgaacgagga atgtcacgac gagacgctgg
     2041 cgcaactgct cagcgaacag gccgggcgcg cggtgaagat cagtcaggcg gccagcgggg
     2101 agcgcaagca atggctggag atggggcagc gcaatgcgat gctggcgctg gagcagcgca
     2161 aggcgcagca gggcgggcaa cagcggcggc tggacaagct gcgcgaatgg ctggacatgc
     2221 ccgcgctgca gcgcatcgag tgtttcgaca tcagccacac catgggcgag gcgacgcagg
     2281 cctcctgcgt ggtgtacgag aatctcgaca tgcggtcggc gcaataccgg cgctacaaca
     2341 tcaccggcat taccgcaggg gacgattatg ccgcgatgcg tcaggcattg actcgacgtt
     2401 accagaagct ttcctcggga gagggcgcgc gccccgatct ggtgttgatc gacggcggtc
     2461 tggggcagct gcatatcgcg atggaggtga tgcaggaact gggcttgaac gacttgcacc
     2521 tggtgggcgt tgccaagggg gtggaacgca aggcgggcat ggaacagctg attttcaccg
     2581 atgggtcgtc gcggcagatg cgcagcgacg atcccgcgct gcatctgata cagcagattc
     2641 gtgacgaagc tcaccgtttc gccatcaccg gacatcgcgc caaacgaggc aaggcccgca
     2701 ctgcgtcctc gctggaagaa ataagcgggg taggggacaa gcggcggcgc agcctgctga
     2761 ctcacttcgg cggtctgcgc gaagtggcac aggccagcgt ggagcaactc tcccaagtcg
     2821 aaggcatcag cacagcgctt gccgaaaaaa tttaccagca actccattag tttcgttgtt
     2881 gaagagagtc accagcaact cgattaaaga acaatcaata ttttgttgct gaaaagattt
     2941 accaacagct acactgagca catgttcaat aacatcccta atctgcttac ctggttaaga
     3001 atcctgctca ttccagtatt cgtggccgtt ttctatctgt ccgataccac gcttgctccc
     3061 cacctgaaga atctcatcag taccagtgtg ttcctgctgg cagcggtgac cgactggctg
     3121 gatggctatc tggcacgcag gcttaaccag tcttccgcct tcggtgcatt cctcgatccg
     3181 gtggctgaca aactgatggt agcggcggcg ctgatcgtac tggtcaaact ggggcgagcc
     3241 gacgcgatca tcgccttcat catcatcgga cgcgagatca ccatttccgc cctgcgcgaa
     3301 tggatggcca agctcggcga aagcaagaac gtcgcggtct cgatgctggg aaagatcaag
     3361 accacttttc agatgattgc gatcctgctg ctgttgtatt acgaccgtct gctgggtatt
     3421 gattgtcagg cgatcggttc cgtgttgctg tacttggcgg ccttgctgac actctggtct
     3481 atgggctatt acttggtgct cgcatcacct caattaaaaa aatctcgaaa ctgaattgca
     3541 ttatcaggct gcatctgtat aatgcgcagc cttcggggtg tagcgtagcc tggtagcgcg
     3601 cctgctttgg gagcaggatg tcgggagttc gaatctctcc accccgacca ggttttagta
     3661 agcgttttgt ttgatcgcct agaaaagaat tgtgcccgta gctcaaccgg atagagcacc
     3721 agccttctaa gctgggggtt acaggttcga ttcctgtcgg gcacaccagt gtctgtcagg
     3781 tgcacggaca aaagttcttt gtcctgcaag tggcttcatc actagcagct tggccgaatg
     3841 actaatgtgt tctgcggcat tagtcggcta gtagttgttg ttcgatcaat ggtggctgta
     3901 gctcagttgg tagagtccag gattgtgatt cctgttgtcg tgggttcgag ccccatcagc
     3961 caccccaaat attcaagagg ccagcctatg ctggcccttt ttatttattc caccgtataa
     4021 tcgcgcgaat gcaccgaatt tgagtgcctc ttcccttatg gccccatggc caatcacagg
     4081 agcgatgatg gttaaatttc ggtacccgcc gctttgggtt gccatgaatg cggtgctgct
     4141 cacctcatca gtctgggctg ctagcgagcc gagcgaacag gactactttc aggaattccc
     4201 ggtcgtactg agcgcctcac ggcttaccca gccacagtcc gaagcaccga atgcgatgac
     4261 ggtgatcgac cgcaagatga tcatcgcctc aggatttcgc aacatcgtcg acttgtttcg
     4321 tctggtcccc ggcatgtatg tgggatacga cagcggcaat gccccctttg tgagctatca
     4381 cggggcgacc gatcagtact cgaggcgcat gcaggtgctg gtggatggac gaacggtcta
     4441 cctgccccca ttcggcggcg ttgattggct ggatctcccg ctgcatatcg atgatatcga
     4501 acgtatcgag gtgatacgcg gaccggcagc ggcatcgcat ggcgccaatt ccttgcaagg
     4561 ggtgatcagc atcatcaccc gcgatgccgc aagtgccagt ggtgcatcag tctctctgac
     4621 caagggtgac gggggaattg ccgatgtagc cgctcgcctg agttcgcatg gcgacaatct
     4681 ggattatcgc gtcacattga cgaaccgctc ggacaacggc tatgacctgc ctgtgctgaa
     4741 cgatggcaac aacaccagtc aaatcaatct gcgcagcaat tatcgattca acgagcggaa
     4801 taatgttgat ttccagttcg gctatagcga agggattcgt ggtgcgggca attcgcagag
     4861 gttacccaat gagccatttc gaaatataaa tacctcgtcc aatttccaac aagttgattg
     4921 gctgcatgca ttgcaggggg gggatgagct caaattgaac tactaccata tcgctcgcga
     4981 ttacaaagat aactcgcctg ttgcgtttga aaaggacaac taccaggttc atcgccatga
     5041 actgggactg cagcatactc tgtctctggg cgactccaac cgcgtcgtct gggggggcgc
     5101 ggtgcgctac gacacggcgg tgagttcctt gtactttaag gaacctcagt cgcagcgttt
     5161 gacacaacta ttcgctcacg acgagttgcg cgtgacatct tccttgctgg ccaaccttgg
     5221 ggcgatgttc gaggatgatg gaatcggaca caaaaatctg tcgccacgcg catcgttgaa
     5281 ttatcacatc acccccgagc acacgcttcg tgcgggagca tcggttgcct accggaaccc
     5341 ggtgatgttc gagcaaaaag ccaatacttc ttatctgggt ggacggcagt atgcttctgc
     5401 cggagtgcaa agaccggaaa aagtgatttc aaaagagatc ggttacctcg gggaactcaa
     5461 ggaaatcggg gttagcctgg atgcaaggat ttatcaggat cgcgtgagcg actatatctt
     5521 tttcgatccg ttgacgcctg cgctattcag tttcagcaac ttatatgcgg ccaattatca
     5581 gggttttgaa agcacgctga agtatcgctg gcgtcagcaa agcaacgtga tcttcaacta
     5641 ctcgcatcag cgcgcaagct gtacggccac cggatcgttg acgatgcccg ccatggtacc
     5701 ggtacttcag gcctttatga atcaatgtcc gctggtggtg ccggcagaca gcggcagcat
     5761 cctgttgacc gaacaattga cccctgatct gcaaattagc gccggttatt atcatcagga
     5821 acccgttcaa gtttttgatg cgatttggtc gcaaaaattg atgcgcagag tagacatgag
     5881 aatggcttat gcattcggac agcgaggtgc gccgggagga ggggaagtcg cccttgtggt
     5941 gcaaaatgca tttcaggaca attacacgga atacaccaac gtcccacaaa aggcggggtt
     6001 tgtatttaac cgtcgtgcct atctgacagc gacgttagca ttctagggac gttatcttgc
     6061 gacgttcttt ggccttgtta atcttgttgg cggctttttt catgctgcca gtggcagcat
     6121 gggccggaga tctgcatgtg ttactgatat tgagtgacaa cactccgccc tatcagtcat
     6181 ttgccaaaaa actgaaggac tctctaccca gttcgattca actgacgatt caggaagcag
     6241 ggaagcctgc cgtcgcctct tctgatctgg tcgttactgt tggcatgaag gctgccgaga
     6301 cggccatctc gaatccgagg gggcccattc tggcggtgat gatccccaag ataggttatg
     6361 acaacctgat cgtagctcga tcaatcccac aagatatctc ggcaatctat ctggatcagc
     6421 catgggggcg acagctcaag tttctctctg cggtcatgcc caatctgaac aaagtcggtt
     6481 tgatttactc gcctgaaaac caccctgaga tatcaacttt gctgaatacc gcgacgagtc
     6541 atggcagcac agttgtcgcg cagatggtgc gatcagaaga cggattgttt gcaagtctcg
     6601 agaaagtttt atcggacagc gatgtcctgc tggccatacc ggatggtcgg atctataacg
     6661 ccagcaatat ccgtaacatc ctgctgacga gctatcgtca caaagtacct ttgattggat
     6721 tctctcaagg ctatgtcaat gcaggttccc tggcagccat cttcaccccc cccgaacaag
     6781 tggcagtaca aacagcaaga caactcattg catttgaaca gaagagggtg cttgcagccc
     6841 ctcgttatcc ggatcagttc accatcgcgg tgaatcttca agtagccaga tcgctgggcc
     6901 tcaccctgcc aagcgaagat ctcatccgca aacgaatgag cgactcaggg gaggggagcc
     6961 gatgaaacgt tttggtatca gcacgcaaat caccgcgttg acactcattc cgttgctggt
     7021 tgttgtaatc agtatggaag ccttcttttt acgggctcgc tttgcagata ttgacgcaga
     7081 tttgctggga catggaaaac tgatggccca ccaacttgcc ggcagtgccg aatacggggt
     7141 tttttccaac aatctggcat tcttggcaag catttccgag aatgccttgc tgcagcgtga
     7201 ggtgcgcagc atcaccatca tggatggtaa tgacaaagtt ttgtttagct ctcggggggc
     7261 tcgtcatcgc gatcttgata ataaagccga gggcaatagc accgaacaga acatatcacc
     7321 cgcccagatc ggcctgttaa atcccgtcaa gaccgacgcc aagagtgtat ggatatatca
     7381 acctatcgtt cccacccagg ttaatctgga cgatatggag ggcatgcata gcgcgcaaca
     7441 gatcggcgcc gtcatcgttg agatgagcag ggccgattcc gaccatcgca aatcggagat
     7501 gctctggttc aatattgccg tgactgcatt gattctggcg ttccctctct acgccatatt
     7561 tttggccagc cgcagcatca ctcaccccat acgccggttg agtaatgctg tattaaaaat
     7621 tgctcaaggt catctggaaa cgcgtgttga cgcctctcgc aaccagacta tcgaactggc
     7681 cagtctatcg agtggcatta atgaaatggc tgcgcaattg caggcggaac gagcaatttt
     7741 ggagcaacgc atcgaggagg ccactctcgc gttgcgcgag aagaaggaag aggccgagcg
     7801 agccagtcat gacaagagtc acttccttgc cgtggccagc catgatctgc gccaacctct
     7861 gcatgccttg gggctttata ttgcagaatt aaaacgaaat tcgaccggcg atgaacagca
     7921 acatctgatc aaacagatag aacgctcagc agaatcgctg tccacgctac tcaatgcttt
     7981 gttggacata tccaaattgg atgcgggtgc cattgttccg caaattcaga gttgtgatct
     8041 gaacttcatg ctcagtcaag tcgccgcaga ttaccagatg ctctcccaga tcaggaacat
     8101 ccggctcgtg gtgcgtgcat gcccgataca tgtcagcagc gaccccatgt tgcttgaacg
     8161 catcctgatg aatctcgtca gcaatgcact tcgttacacc cctcaaaatg gctgcgtaat
     8221 gatcgcatgc cgccaccgaa ataatcattt gcgtatcgaa gttcgagata acggcattgg
     8281 aattaccgcg agcgatcagg ccaacatctt ccgtgagttc tttcagattg ctcagccgca
     8341 gctagattcc agcaaaggac tgggcttggg cctagccatt gtggatcggc tggcaaagtt
     8401 gcttggtcac aagattgagt tacgatcggc tcccgctaaa gggacagttt ttgcagtcga
     8461 ggtgccgata gccgctacac tcgcaggcga agcctcattt atatcgtccg ggcgcgatct
     8521 ggaaccggag ggattgatcg aacattcgcc attaacggga aagcggctgc tagtcgttga
     8581 tgatgacgat atggttttga acagcacggc cagtattctg acttcatggg gttgcaaggt
     8641 taccttagcc acgtctctgc cagaagttga acgccacctg caagacggca tgcactggga
     8701 catgatcatc agcgactacc agctggaaaa ccatacgacc ggaatagatg tgattgatca
     8761 ggtacggcga caccttgcta aagagcttcc atgcattctg attagtggcg ataccggccc
     8821 ggcagtgcta aaactagccg cagtgggcgg atatcacttg ctgcataagc ctgtaaaacc
     8881 cgcaaaactc agatcgttag ttcttcattt gctggatgag gaaaagtagt cgtgccaaga
     8941 atcgttccag gctacctcct tctgatattc gcataatgta tattcccctt gattcgaggt
     9001 cgtgactgtg cctctcttcg ccataatttc ccaagtgtaa aatggctgct tctgctggtt
     9061 ttggagcgat agcaatggtg caggctaggg cggtcattgc gataactgcg gtaatgcctc
     9121 ggctttcaat gtaagttttc cagaa
//
LOCUS       DASUIV010000102        19535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_859607, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000102 DASUIV010000000
VERSION     DASUIV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 19535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19535
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859607"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1676)
                     /locus_tag="VFW59_03735"
     CDS             complement(66..1676)
                     /locus_tag="VFW59_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5363354.1"
                     /translation="MKISFRLTWLLVSLLGLSLVIGAVGLYATNVQERAVEVIYSNNL
                     EPIRNLSKAQERTLHTRLALTNAVLHPENGDKYLKEVADNLALVQKSLDEYASSGLTP
                     EDKVVVDKMLAANKAFVDGAINPAIEMVQRADYAGLRMHLDDKMRPLYYALNAELDQL
                     ITLQEREARGSKDAADATSKQMFALSGGLIVVGSILGTLLGLSIIRGINRSVAEMSGV
                     MGRMAGSGDLTERARVYGSDEVALAATSFNRLIDSFANILRQVSANAVTVSDSAAQLS
                     SSSLQISEGSQAQSEAAASTAAAVEEITVSINSVAANTEDVRKLSEQSLEQTQLGNRN
                     VDEMIGEIRLVQESVNQIAGSVKEFVESTHAIAGMTQQVKDIAEQTNLLALNAAIEAA
                     RAGEQGRGFAVVADEVRKLAEKSAQSASEIDQVTNSLNQKSTHVEGTVQAGLRALLTT
                     QEHIERVATVLSAASAAVTQSSHGVNDIASSVAEQGLASNEISRNVERIAQMAEENHA
                     AVQSNGQEIVRLEQLAGELRSAVSRFRV"
     gene            complement(1750..3375)
                     /locus_tag="VFW59_03740"
     CDS             complement(1750..3375)
                     /locus_tag="VFW59_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5363355.1"
                     /translation="MNNWWSRLSLKNKLQIPIQLLLLAIMVIAQRSALNTFEGHVLDG
                     SKQKVAVSADGVLNGLNMLMVTGAISDADNRALFIKKMSSSDGIVELRVMRGKPVQDQ
                     FGPGMPAEQPVDELDRKALTTGKLQTEMLDRNGVRALRVVVPFIAKEEFRGTKCLACH
                     IVPAGSINGAASITVDLTEEYALMSKANAMLWAAQLVIQVVLYFTIGWLIDLVIRPTR
                     ELQKVMQTMQADGDLTKRATVRSQDEIGKTAQAFNDLTSGFQVIVTQVEGHASRVNEA
                     AHSLAKDAGELAQGAQQQSDTAASTSAAVEQVSASIGRVAEGAERVAKLSTESLERAN
                     RGQESLHEMVQELERVESAVGQIAASVGEFVSSTQSITSMTQQVKAIAEQTNLLALNA
                     AIEAARAGEQGRGFAVVADEVRKLAEKSAQSANEIDEVTQSLGAQSEQVEKAMQRGMS
                     ALQSSQAHIREVTGVLVEANTSVGGVNRGLEEISESINQQRDASQEIARNVDAIAVMA
                     SRSNEVVKRTVNEVQAMEQLSSNLSQTVGRFKV"
     gene            complement(3522..4472)
                     /gene="motB"
                     /locus_tag="VFW59_03745"
     CDS             complement(3522..4472)
                     /gene="motB"
                     /locus_tag="VFW59_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX5363356.1"
                     /translation="MSQDKNKKPIVVKRVKKVAGMAHGGAWKIAYADFVTAMMAFFLL
                     MWLLGSTATGDLKGVAEYFKTPLKVALLGGTSSGESSSIIKGGGGDITRQTGEKQTIV
                     PANKAKTPDVQAIQQEIARIEHLQEVEKLKQLKRNIEKAIENSPSLKKFKNQILLDMT
                     TEGLRIQIVDEKNRPMFQLGSAELEPYMRDIMHEIGRMLNEVPNKVSLSGHTDAALFF
                     GGSKGYSNWELSADRANASRRELIYGGMESEKIVRVVGLSSAVLFNKSDPLNPMNRRI
                     SLIVMNKKAEEAAQHDGGVVEDVAEQGAASGVPTVPQAHE"
     gene            complement(4577..5431)
                     /gene="motA"
                     /locus_tag="VFW59_03750"
     CDS             complement(4577..5431)
                     /gene="motA"
                     /locus_tag="VFW59_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX5363357.1"
                     /translation="MLVIVGYIVVSLSVFGGFALAGGHMGALFQPVELLMIGGAATGA
                     FLVGNTSASIKSTLASLPKVFKGARYNKAVYMELLALLYELLNKVRKEGLMSIEGDVE
                     EPHESQIFAKYPGVAGDHHVVEFMTDYLRVMVSGNLNAMEIENLMDVEIETHHHEALA
                     PSHIIAKLGDAMPAFGIVAAVMGVVHTMESVGIPPAELGILIAHALVGTFLGILLAYG
                     FVGPLASTLEQKADESTRIFNVIKVTLLANLNGYAPAMAVEFGRKVLVSRDRPGFLEL
                     EEHVKQKR"
     gene            complement(5541..6128)
                     /gene="flhC"
                     /locus_tag="VFW59_03755"
     CDS             complement(5541..6128)
                     /gene="flhC"
                     /locus_tag="VFW59_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HEX5363358.1"
                     /translation="MKSSHNRAGDPVVKTVAGEARQVQVAIDLIKLGARLQLLQEETS
                     LSRERLLRLYKEIKGESPSKGMLPYSTDWFMSWQPNIHASLFMDIYRFLVMNAGIEGV
                     QALITAYRLYLDQMQGIEESEPLLSITRAWFLIRFFKAGMMQLSPCKECGGYFVTHIN
                     ELHDHYVCGICHPPARAGKTRAAKQAALDSAASEP"
     gene            complement(6112..6429)
                     /gene="flhD"
                     /locus_tag="VFW59_03760"
     CDS             complement(6112..6429)
                     /gene="flhD"
                     /locus_tag="VFW59_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEX5363359.1"
                     /translation="MDSMQLQEGIRELNLTYLVLAQQMIKEDRAAAIFRLGISQDLVD
                     VITGLSPAQVVKMASSNMLLCRFRFDETLLLNMVSDYSKDRMMSRAHAAILMSGQPIE
                     ELA"
     gene            complement(6653..8575)
                     /locus_tag="VFW59_03765"
     CDS             complement(6653..8575)
                     /locus_tag="VFW59_03765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5363360.1"
                     /translation="MDVDENSVLTRLKAADRLPAPKGVALEVINLTQRDDVSNHDIVR
                     LISVDPALCARVIKAANVLLSNPSRPIATISDAVTVLGARALRQLVMGIALIVDYRSG
                     PCKQFDYQHFWAHSLLTGIAARHLVARTRLAIPEEIFVLGLLGNIGQLAFATAYPEEY
                     GELLGRASESPVDELCRLEQGEFGCDETELSGAILADMHFPMLFQTLAHDHLLPDSSK
                     LEEGSREWQLLHLLYVASLLADACLARQAQRGGLVAKLRQSAARVGVEGDRLIEIGDA
                     CTQDWLDWLDMLGMKMVRIPPFTELLQQPEVADDEIDVPPWIDVGRDHYKMRVLVVDD
                     DRAMLGLLAAMLKAAGHEVLTAHDGVEALRLVEQASPQLVITAWGLPEMDGLELCRKL
                     REDVLRRDVYVFILAPEESPDRLVEAFEAGVDDYLVKPFSQRVFFARLRAAQRVVQLQ
                     DELAYDREQLMRFSRDLATSNERLQRLALTDALTELPNRRFAMERLEQEWALSLRGDR
                     ALSCMMVDIDHFKAINDRLGHQAGDEVLKSVAHALRQAARTQDQVCRYGGEEFVVICP
                     DTGLDAAVQCAERLRQMVFKLICKSGATNLSLTISIGVAEHRPEMVSVDDLLARADAC
                     LYAAKEGGRNRVVSGK"
     gene            complement(8730..8806)
                     /locus_tag="VFW59_03770"
     tRNA            complement(8730..8806)
                     /locus_tag="VFW59_03770"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8770..8772),aa:Met,seq:cat)
     gene            complement(8846..9565)
                     /locus_tag="VFW59_03775"
     CDS             complement(8846..9565)
                     /locus_tag="VFW59_03775"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5363361.1"
                     /translation="MMRTVFWILLLVNVVFFAVMQWGVALVGEPPLQAQPVLHEDKVQ
                     LLAASQVAALPPIPVAASAVPSPSSAPVATPASSTPPKHEARVCLEWGEFSGPELVRI
                     KAALAGLKLGDRLGRREVEYSNGYWVYIPSLKDKASVAQKIEQLKARGVEEYFVVQEA
                     GPLFNAISLGVFKTRDAAQKFVNDLHARDVTSAQVGERNSKLKATVFLLSGVDAATST
                     RLIGMYKDFPGSDQKEVPCTH"
     gene            complement(9562..10278)
                     /locus_tag="VFW59_03780"
     CDS             complement(9562..10278)
                     /locus_tag="VFW59_03780"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5363362.1"
                     /translation="MRLLIDAGNTRIKWALTQGDAWLRSGVLPVERVGELFAQFADAP
                     DIQDVWVSNVAGEAVAQAICNSVAPDKLHFIVACERQCGVRNGYAEASRLGSDRWAAL
                     IGAWHLVGGCCLVVNCGTATTVDALSENGEFIGGLILPGVELMQRSLCGAAAQLQAAS
                     GAYAAFPCNTADAMYSGVVQASCGAIERQHGLLGVARAEVVLSGGAAEVLRERLNLPL
                     RLVDNLVLQGLWLIAQETKG"
     gene            complement(10278..11261)
                     /locus_tag="VFW59_03785"
     CDS             complement(10278..11261)
                     /locus_tag="VFW59_03785"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5363363.1"
                     /translation="MSAGGLRARTWQLLDILADGHFHSGEVLAGQLGVSRASVFNALA
                     VAADCGVTVQRVRGRGYRLAQPWQRLQRDEVAGWLGEESGRFDIEIVPQTASSNTLLL
                     QRAALGAPGGSVVAAELQSAGRGRLGRTWHSGLGNALTFSLLWRFDCGLNALSGLSLV
                     VGVAVVRALNKAGVQGVGLKWPNDILCAQGKLGGVLLEAQGDMLGPSAVVVGIGLNCH
                     LPESLVRQIDQPACALDELCAALPPRNRLLALLLLELSAVLSQFGRDGFGPFRGEWER
                     YHLYQDRPVQLCMADGSVVSGIARGVGEVGELLLETAQGLRSFHSGEVGVR"
     gene            complement(11258..12424)
                     /locus_tag="VFW59_03790"
     CDS             complement(11258..12424)
                     /locus_tag="VFW59_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5363364.1"
                     /translation="MGERVGLILTGGGARAAYQVGVLQAIAECLPRHAHNPFPVICGS
                     SSGALNAAALAVNAHNFRRSVRYLRNIWQNFHTDQIYRTDVVGVFNNTALWLAGLVLS
                     GLGINRLDQVSLLDNAPLAELLDEILPCEKIQHNIDAGLLHALSITASGYESGESVTF
                     YQGKSDIKPWKGARRVGVPTDIEVKHLLASAAIPFIFPARLINREYFGDGSMRQIAPI
                     SSALNLGATRILVVGATNDALEQSRRAVEGSYPSLAQIAGHALNSLFMDSMEVDLERV
                     QTINELVKIMPEEMRKMTGLKYVELLVIKPSESIDKIAERYMSELPWTIRVLLRLIGA
                     APRSGGSALISFLLSEKKYCRALIELGYSDALQQREEIMRFLGESLCQKDRDTQ"
     gene            complement(12426..12659)
                     /locus_tag="VFW59_03795"
     CDS             complement(12426..12659)
                     /locus_tag="VFW59_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363365.1"
                     /translation="MNKQEIELLRQEVEMLMVERMRLLKVVGAAAVLVSKSEVRALPK
                     EAVEAADALSELLNALSEETLKDALDSVRGELR"
     gene            complement(12732..14918)
                     /locus_tag="VFW59_03800"
     CDS             complement(12732..14918)
                     /locus_tag="VFW59_03800"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX5363366.1"
                     /translation="MDKQFREAALQYHRQTPAGKISVVPSKPMQTQRDLALAYSPGVA
                     AACEEIVRDPNEARNLTARGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGVLFKKFA
                     GIDVFDIEVAENDPDKLVDIVASLEPTFGGINLEDIKAPECFYIERKLRERMKIPVFH
                     DDQHGTAIIVGAALLNGLKVVGKDIGKIKLVVSGAGAAALACLDMLVGLGVSMANITV
                     TDIKGVVYKGRTEEMDDNKARYAVETSARTLGEVIAGADVFLGLSAGGVLKPEMVVQM
                     GDRPLIFALANPNPEILPEHAKAVRPDAILATGRSDYPNQVNNALCFPYMFRGALDVG
                     ATTINEEMKRAAVYAIAELACAEQSHAVSSVYGEDEQSFGANYLIPKPFDQRLIKRVA
                     PAVAQAAMDSGVATRPIADMAAYREQISHFVYQSGLLMKPVFDTAKRDPKRLVYAEGA
                     DERVLHAVRTVVDEKLAKPILVGDAATIVRRIDKLGLRLVAGEHFELVDCAGDTCHPT
                     LVAARLVSEGRADAMLCGVTDQPQDHLRHVREVIGLREGASVLAAMNILMLKEHTLFI
                     CDTHVNLDPTAEQLAELTQLAAAEVRRFGIEPKVALLSHSNFGSFDDASARKMSRARE
                     LLEQQAPGLEVEGEIHGDAAVSAAIRAQLFPDSRLKDNANLLVMPNLDAANIAFNLLK
                     VLAGDGITIGPVLLGAAKSVHIMTNTATVRRIVNMSALAVVGVKTH"
     gene            15152..16363
                     /locus_tag="VFW59_03805"
     CDS             15152..16363
                     /locus_tag="VFW59_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5363367.1"
                     /translation="MYPALTPQRERFLLLALAGIQFSHVIDFMIMMPLGPILMIELGI
                     TTHEFGLLVASYRFTAALSGLLTAIFVDRFERKRMLLTMFALFGIATLACGLAPGYAT
                     LLVARGLAGAFGGVMGALTQTIVGDVIPFARRARAGGMVSASFSVATVAGVPLSLWLA
                     NHFQWRAPFIFIAILVVLFIIVGLRVLPELRGHIATEKRAHPFAPMFDVLRDANHLQA
                     LLFSALIIFSGFTVIPYITVFAVSNIGISQHDIPYIYLFGGAATLVTARLIGHWADLY
                     GKVRVYRIVAAAAMLPLLVVTHLGETPLWFWIICTTVFFVMVSGRMIPAMAIITSAAQ
                     PRLRGTFMSINATIQSLAMGLATTLAGFIIAQDADGKIVHYSTVGYVAVTANLLAIWF
                     VGRIAMHDQHK"
     gene            16436..18145
                     /locus_tag="VFW59_03810"
     CDS             16436..18145
                     /locus_tag="VFW59_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX5363368.1"
                     /translation="MTWKLLINRIQQWREHLAERAAQLLKYSFYLYLAGLFSVFAVLD
                     TTMLHFTSEMRQAAFDTMVRYRIVVPKPDPEIVIVDINEASLAAMAQDYGRWPWPRQV
                     LGEFLEQIEKQQPKAVVFDILFSDADVYNPDSDTYFEAAIANTRNTYFPMLRLDPASD
                     SLSQIKPAMIPGVRPLPGTQPDDAATIAVVLPHFQAALDGGRLGLHNIYPDSDGVARR
                     YNVQRDDYGWALPSLPTRVARELGWPEPDAQQVLLNWRGQPFTYRYVSFSDVYADMGS
                     KDKKRPQDEFKNKIVIIGSTAPSLFDIKATPMNRMFPGVEILATAIDNFKHGDALRFP
                     EGRMWYLLITLTIIWLTAWGFYRDAGRDKIDRVFGLSQFILIGITYASINFTNVYINL
                     TGPVTIGLAFFSLARLYAMATGKALEQNMVRTATARTGELQATLLLIRLDNERNAIRP
                     VAQEKIRHALEKTGADNKSVDVLTGDQKGLWDLFENTLAISWVAEAHNAEAQAAIRRD
                     IDALLAALPGVLQQHLARPDNAASHFIHQGRIDGGAAAAAGWRVMFAEALLEWDKKEN
                     EQS"
     gene            18142..18636
                     /locus_tag="VFW59_03815"
     CDS             18142..18636
                     /locus_tag="VFW59_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5363369.1"
                     /translation="MKKSRFGLTLAALLLSGALHAAESGTALKADEIKAEPFRDAKTV
                     AKLATGDKVDILKKDGGWFQVSSAKGRGWVRMLSIRKGEAGKGSGAGGLLAMTSGRAG
                     TGKVVSTTGIRGLNEEELKAAKFDEAELKRAESYATNKSAAAKFAAQGKLAARPFDYL
                     PAPK"
     gene            18651..19475
                     /locus_tag="VFW59_03820"
     CDS             18651..19475
                     /locus_tag="VFW59_03820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5363370.1"
                     /translation="MNHKTFWILALALFSANALAANPFEQQLFDALKQNVQEKVTGKS
                     ATSVEEETAIGRQIAGNLLGAAPLVKDAKLQKYVNNVGRWVADQSERPDLVWHFGVIA
                     SNDVNAFAAPGGYIFVTKGLYKLLQSESELAGVLAHEVGHVIRQHHLKILQQSKLLDL
                     GSQAIAQNVGGNDRVQGLIGSGAEIVSRSLDKGAEFEADRIAVVLATRAGYDAFGLPG
                     VLQQIGHFAKDDNDVALLFKTHPHPDDRLGKLGAAMDDRFDKVKGKTLEKRLYRVK"
BASE COUNT         3958 a         6637 c         5240 g         3700 t
ORIGIN      
        1 gcatgcccac ctctcaacct gtgaaatccg cgtatccccc ttacaggaac gcgctcgccg
       61 tgcccctaca ccctgaatct gctcactgcc gaacgcaact cgccagccag ttgctccagc
      121 cgcactatct cctgaccatt cgactgcacc gccgcgtgat tctcttccgc catctgcgcg
      181 atcctctcca cgttgcgcga aatctcgttg ctcgccaaac cctgttcggc caccgacgaa
      241 gcgatgtcat tcacaccatg gctggactgt gtaacggcgg cactcgccgc gctcaacacc
      301 gtcgccacgc gctcgatatg ctcctgagtc gtcagcaacg cgcgcagccc tgcctgcacc
      361 gtaccctcga cgtgggtcga cttctggttc agcgaattgg ttacctgatc gatctcgctg
      421 gccgactgcg cagacttctc ggccagcttg cgcacttcgt ccgccaccac cgcaaagcca
      481 cgcccctgct caccggcgcg ggcggcttca atagcagcat tcaaggccag caagttggtc
      541 tgctcggcaa tatctttcac ctgctgcgtc atgccggcga tcgcgtgggt gctctcgacg
      601 aactccttga ccgaaccggc gatctgattg accgactcct gtaccagacg aatttcaccg
      661 atcatctcgt ccacattgcg atttcccagc tgagtctgct ccagactctg ctcggacagc
      721 ttgcgtacgt cttcggtatt cgccgcgacc gaattgatac tcaccgtgat ctcttccact
      781 gccgccgccg tggaggcggc ggcttcactc tgcgcctgcg aaccttccga gatctgcaag
      841 gaggagctgg atagctgtgc cgccgaatcg gagaccgtga ccgcattggc gctcacttgg
      901 cgcagaatat tggcgaagct atcgatcaga cgattgaacg aagtggccgc cagcgcgact
      961 tcgtcgctgc catacaccct ggcacgttcg gtcagatcgc cgctgcccgc catccgcccc
     1021 atcacgccgc tcatttcggc caccgaacga ttgataccgc gaatgatcga caggcccagc
     1081 aatgtaccca gaatcgagcc caccacgatc aatccgcccg aaagggcaaa catctgcttg
     1141 gatgtcgcat ccgccgcatc cttgctacct cttgcctctc gttcctgcaa cgtgatcaat
     1201 tgatccaact cggcattaag tgcgtaatac aagggccgca ttttgtcgtc cagatgcatc
     1261 cgcagcccag catagtctgc gcgctgaacc atctcgatcg ccggattaat cgccccgtcg
     1321 acgaaagcct tgttcgccgc aagcatcttg tcgaccacca ccttgtcttc cggagtcagg
     1381 ccggaggacg cgtattcatc cagagacttc tggaccaacg ccaggttgtc cgcaacctcc
     1441 ttgagatatt tgtctccatt ctccggatgc aataccgcat tggtcagcgc cagacgggta
     1501 tgcaatgtcc tttcctgagc cttactcagg ttccggatag gctccagatt gttgctgtag
     1561 atgacttcta ccgcccgctc ctgaacgttg gtggcataca agccgaccgc accgatcacg
     1621 agggacaacc ccagcaacga gaccaacagc caggtcaacc gaaaactaat cttcatattg
     1681 ctcaacatac acacacccca attaaataac tctcacccga caaaaccgga aggccgcaaa
     1741 cggcacgacc tacactttaa agcgccccac cgtctgactc aggttggacg agagttgctc
     1801 catcgcctgc acctcgttca ccgtgcgctt gaccacttcg ttgctccggc tggccatcac
     1861 cgcgatcgca tcgacgttgc gggcgatttc ctggctggca tcgcgctgct ggttgatgga
     1921 ttcggatatc tcttcaagcc cgcgattgac gccgccgacc gaagtgttcg cctcgaccag
     1981 cacccccgtc acttcgcgaa tatgcgcctg actgctctgc aaggcgctca tgccgcgctg
     2041 catggccttt tccacctgtt cggactgcgc acccagcgac tgcgtcactt cgtcgatctc
     2101 gttggccgac tgagccgact tctccgccaa cttgcgcacc tcatccgcca cgaccgcgaa
     2161 tccgcgcccc tgttcgcccg cacgcgccgc ttcgatcgcc gcgttcaatg ccagcagatt
     2221 ggtctgctcg gcgatcgcct tcacctgctg ggtcatgctg gtgatgctct gcgtactgct
     2281 gacgaactcg cccaccgagg cggcgatctg tccgaccgca ctctccacgc gctccagctc
     2341 ctgcaccatt tcatgcaggc tttcctgacc gcgattggcg cgctccagac tttcggtgga
     2401 gagtttcgcc acccgctccg ccccctcggc aacgcgccct atgctggcgc tgacctgctc
     2461 gaccgctgcc gacgtcgagg ccgccgtgtc gctctgttgt tgtgcgcctt gcgccaactc
     2521 tccagcatcc ttagccagcg agtgtgccgc ctcattcaca cgcgaggcat gcccctccac
     2581 ctgggtcacg atcacctgaa aaccggaggt cagatcgttg aatgcctgcg ccgtcttgcc
     2641 gatctcgtcc tgactgcgca ccgtggcacg cttggtcaga tcgccgtctg cctgcatggt
     2701 ctgcatcact ttctgcagct cgcgggtcgg acggataacc agatcgatca gccagccgat
     2761 ggtgaaatac agaactacct gaataaccag ttgcgccgcc caaagcattg cgttcgcctt
     2821 gctcatcagg gcatattctt cggtcagatc caccgtgatg ctggccgcac cattgatcga
     2881 accggcgggg acaatatgac aagccagaca cttggtgccg cgaaactctt ccttcgcgat
     2941 gaacggcacc accacgcgca aggcgcgcac gccgttccgg tccagcatct cggtctgcag
     3001 cttccctgtg gtcagcgcct tacgatccag ctcgtccacg ggctgctcgg cgggcatgcc
     3061 gggcccgaac tgatcctgca ccggcttgcc gcgcatcacg cgcagctcga cgattccgtc
     3121 cgaagagctc atttttttga tgaacaaggc gcgattatcg gcatcactga tcgccccggt
     3181 gaccatgagc atattcagcc cgttgagcac gccatccgcc gaaacagcga ctttctgctt
     3241 cgagccatcc agcacatgcc cctcaaaggt gttcagcgcc gaacgttgcg caatcaccat
     3301 gatcgccagc aacagcagct gaatgggtat ttgcaacttg ttcttgagcg atagcctgct
     3361 ccaccagtta ttcatcccgc gctcccttat agatcaataa tgcgattttt atcctgctcc
     3421 ggccagttta acgaccacag cattcatctc atgcaacgaa tacaccggta aaaccccgat
     3481 caattctcgg cttcaacatg actgcggttc tgagatttca atcactcatg cgcctgcgga
     3541 acagtcggca caccagacgc agccccctgc tcggccacat cttcgaccac ccccccgtca
     3601 tgctgcgccg cctcctccgc cttcttgttc atcacaatca gactgatgcg gcgattcatc
     3661 ggattcagcg gatcgctctt attgaacagc acggcggaag acaggcccac cacgcgcacg
     3721 atcttttcac tttccatgcc gccatagatc agctcgcggc gcgaggcatt ggcgcggtca
     3781 gcggacaact cccaattgct gtagccctta ctcccgccga agaacagcgc ggcatcggta
     3841 tggcccgaca ggctcacctt gttaggcacc tcgttcagca tccgtcctat ctcatgcatg
     3901 atgtcccgca tgtagggctc cagctcggca ctgcccagct gaaacatcgg acgattcttt
     3961 tcgtcgacga tctgaatacg caacccctcg gtggtcatat ccagcaggat ttggttcttg
     4021 aattttttca ggctggggct attctcgatc gccttctcga tgttgcgctt caactgcttg
     4081 agcttctcga cttcctgcag atgctcgata cgcgcgattt cctgctgaat cgcctgaaca
     4141 tcaggcgtct tggccttgtt tgccgggacg atcgtctgct tctcacccgt ttgccgggtg
     4201 atgtcaccac cgcccccctt gatgatgctg ctggactcgc ccgaactggt gccgcccaac
     4261 agcgccacct tgagcggcgt cttgaaatat tcggcgacgc ccttcaggtc cccggttgcc
     4321 gtcgacccca gcaaccacat caacaggaag aacgccatca tcgcggtgac gaagtcggca
     4381 taggcgattt tccacgcccc gccatgcgcc atgcccgcaa ctttcttgac gcgttttaca
     4441 acgatgggtt ttttattttt gtcctgactc atgcgccgaa tatcaccaac ataaatctgg
     4501 acggatgctc acacccgagg ccaagcgaaa aaatgctccc gccccgatga cgaaaggcac
     4561 gatcgatcgc ttacgactaa cgcttctgct tgacatgctc ttccagttcg aggaagccgg
     4621 gacgatcgcg ggaaaccagc accttgcgcc cgaattcgac cgccatcgca ggcgcgtagc
     4681 cgttcaggtt ggcaagcagc gtcaccttga tcacgttgaa tatccgcgtg ctctcgtcgg
     4741 ccttctgctc cagcgtgctc gccagaggac cgacgaaacc ataggccagc aggataccca
     4801 ggaaggtgcc gaccagcgca tgggcgatca ggatgcccag ctcggccggc ggaataccga
     4861 ccgactccat ggtatgcacc acccccatca ccgcagctac gataccgaac gcgggcatgg
     4921 catctcccag cttcgcgatg atatgagagg gcgccaaggc ttcgtgatga tgcgtctcga
     4981 tctccacatc catcaagttc tcgatttcca tggcattgag gttgccgctg accatgacgc
     5041 gcagatagtc ggtcatgaac tcgaccacat gatggtctcc cgcgacgccc ggatacttgg
     5101 cgaagatctg gctttcgtgg ggctcttcca catccccctc gatagacatc agcccctctt
     5161 tgcgcacctt gttgagcaat tcatacagca gggccagcaa ctccatgtag acggccttgt
     5221 tgtatcttgc ccccttgaat accttgggta acgatgccag agtggatttg atcgacgcgc
     5281 tggtattgcc gaccaggaag gcccccgtcg cagccccgcc gatcatcagc agttcaacgg
     5341 gctgaaacaa cgcccccata tgcccgccgg cgagcgcaaa gccgccaaac accgaaagcg
     5401 aaactactat gtaaccgaca atgaccaaca taactgcgcc ccctgttgta cccgccccgc
     5461 atcaccatca cgagcgcatt cgacaaatct atcggcgcat tctaaatcaa atttacccca
     5521 ggaggggcag cagccaagga ttagggctcg gaggcggcgc tatccagcgc cgcctgtttg
     5581 gctgctctgg tcttgcctgc acgagccggc ggatggcaga taccgcacac atagtgatcg
     5641 tgcagttcat tgatgtgagt aacgaagtaa ccgccgcatt ccttgcatgg cgagagctgc
     5701 atcatcccgg ccttgaaaaa gcgaatcaag aaccaggcgc gcgtaatact caagagcggc
     5761 tcggactctt cgatcccctg catctggtcg aggtacagac ggtaagccgt gatcagcgcc
     5821 tgcacccctt cgatgccggc attcatgacc aggaagcggt aaatatccat gaacagggaa
     5881 gcgtgaatat tgggctgcca actcatgaac caatcggtcg agtacggcag catccccttg
     5941 gacggggact cccccttgat ttccttgtaa agccggagca ggcgctcacg gctcagcgag
     6001 gtctcctcct gcaacaactg aagacgcgcc ccaagcttga tcaggtcgat tgcgacctga
     6061 acctgacggg cctcgcccgc tacggtttta acaaccggat ctcccgcccg attatgcgag
     6121 ctcttcaatg ggttgccccg acatcagaat cgccgcatga gcacgagaca tcatgcgatc
     6181 cttgctgtag tcgctgacca tattgagcaa cagcgtctcg tcgaagcgga agcggcacag
     6241 cagcatgttc gaagaggcca tcttcaccac ctgggctggc gacagaccgg taatcacatc
     6301 gaccagatcc tgactgattc ccagacgaaa gattgcagct gcccggtctt ccttgatcat
     6361 ctgctgagcc agcaccagat aagtcaggtt gagctcgcgt ataccttctt gcaattgcat
     6421 gctgtccata taacctcctg tagatccttc gcagacagac cttcgtcatc tgccagaaca
     6481 cgttcattgc gaacgtacgc caccgaatca tgacgcctgc attatttcgg aaccgggggg
     6541 agagggtaat cggaaggggg ccaattattt tgtaggggga ccgacggggg cttgtctggt
     6601 tttttcctac accgacgcat cccgcatttc ggacagattg cattggacgc gcctacttcc
     6661 cggagaccac cctgttgcga ccgccctcct tggccgcata caaacaggca tcggcgcgcg
     6721 ccagcaaatc atccaccgaa accatctccg gacgatgctc cgccacgccg atgctgatcg
     6781 tcaacgagag gttcgtggcg ccgctcttgc agatcaactt aaacaccatt tgacgcaagc
     6841 gctcggcaca ttgaacggcc gcatcgagcc ccgtatccgg acagatcacc acaaattcct
     6901 caccgccata gcggcacacc tgatcctgcg tgcgtgccgc ctgacgcaag gcatgcgcca
     6961 cactcttcaa gacctcgtca cccgcctgat gccccagcct gtcgttgatg gccttaaagt
     7021 gatcgatatc gaccatcatg caggacaggg cgcgatcgcc acgcaacgac aacgcccact
     7081 cctgctccag acgctccatc gcaaagcggc gattgggcag ctcggtcaac gcatccgtca
     7141 acgccagtcg ctgcaaacgc tcgttggagg ttgccagatc ccgggaaaag cgcattaact
     7201 gttcgcgatc gtaagccagc tcgtcctgca attgcaccac ccgctgagcg gcgcgcaaac
     7261 gcgcaaaaaa cacccgctgc gaaaacggct tgaccagata gtcatccacc ccagcctcga
     7321 acgcctccac caagcgatca ggggattctt caggggccag aataaaaaca tagacatcgc
     7381 gacggagcac atcctcgcgc agcttgcggc aaagctcgag gccatccatc tccggcaacc
     7441 cccaggcggt gatcaccaat tgcggggagg cttgctcgac aagccgcaat gcttcaacgc
     7501 catcatgagc cgtcaacacc tcatgccctg ccgccttgag catagccgcc agcaagccca
     7561 gcatggcccg atcatcgtcg accaccagca cacgcatctt gtagtgatcg cgcccgacat
     7621 cgatccaggg cggaacgtcg atctcatcgt ccgccacctc cggctgctgc aacagctcgg
     7681 tgaacggcgg gatgcgcacc atcttcatgc ccagcatatc cagccagtcc agccaatcct
     7741 gagtgcaggc atcgccgatc tcaatcaacc ggtcgccctc tacccccact cgcgccgcag
     7801 actgcctcag cttggcaacc agcccgccgc gctgcgcctg tctggccagg caggcatccg
     7861 ccagcagcga tgccacatac aacagatgca gcaattgcca ctcgcgcgag ccttcctcca
     7921 gcttgctgct atccggcaac agatgatcat gagccaaggt ctgaaacaac atgggaaaat
     7981 gcatatcggc gaggatcgcg ccgctcagct cggtctcatc gcaaccgaac tcgccctgtt
     8041 ccagccgaca caactcatcc accgggctct ccgatgctcg ccccagcaat tcgccatatt
     8101 cctccggata ggccgtagcg aaggccagct ggccaatatt gccgagcagc cccagcacaa
     8161 atatctcctc gggaatcgcc aatcgcgtgc gtgccaccaa atgcctggcg gcaataccgg
     8221 tcaacagcga atgagcccag aaatgctggt aatcgaattg cttgcagggg ccactgcgat
     8281 aatcgacgat cagcgcaata cccatcacca gctggcgcaa cgctcttgcg ccgagcacgg
     8341 tcaccgcatc cgagatggtc gcaatcggcc tggagggatt cgacagcagc acgttcgccg
     8401 ccttgatgac gcgcgcgcac agcgccggat cgacgctgat cagccgcacg atgtcgtgat
     8461 tggatacatc atcacgctgc gtcagattga tcacttccag cgccacgcct ttcggcgcag
     8521 gcaaccggtc ggcggccttc aaacgcgtca gtactgaatt ctcatcgaca tccattgctt
     8581 tcccctcgtt ctgagggagt ctaccgcaac gaggcatgat ttcacagggc tgcggcctgc
     8641 cgcatcagac gaaaaaagcc cgcctgcatg acacagacgg gctttttgtc ttgcccagat
     8701 atctttaaca agacatatcg gccagtattt ggtggtgata ggtggatttg aaccaccgac
     8761 cccagcatta tgagtgctgt gctctaacca actgagctat atcaccgcga agagcgcggc
     8821 attctccgga tttcggacct gcttgtcaat gcgtacaggg aacttccttt tgatcgctgc
     8881 cgggaaaatc cttgtacatg ccgatcaacc tggttgatgt cgccgcatcc accccgctca
     8941 gcaagaacac cgtcgccttc aacttgctgt tgcgctcgcc gacctgcgcc gaggtcacat
     9001 cccgcgcatg caggtcgttc acgaatttct gtgccgcgtc ccgcgtcttg aacaccccca
     9061 gcgagatggc gttgaatagc ggcccggcct cttgcacgac aaagtattcc tcgacgccgc
     9121 gcgccttcag ttgctcgatt ttctgcgcga cactcgcctt atccttcagc gacgggatgt
     9181 acacccaata gccattgctg tactcaacct cgcgccgccc cagccgatct cccagcttca
     9241 acccggccaa cgccgccttg atgcgcacaa gttcaggccc ggaaaattcg ccccattcca
     9301 gacagacgcg agcctcatgc ttgggaggcg tcgaggaggc gggcgtcgcc acaggtgccg
     9361 acgacggaga gggcacagcc gatgcagcga cggggatggg cggcagcgcg gcgacttgcg
     9421 acgcagccag cagctgcacc ttgtcttcgt gcaggactgg ctgcgcctgt aacggcggtt
     9481 cgccgaccag ggcaacgccc cattgcatca ccgcgaagaa gaccacattc accagcaaca
     9541 aaatccagaa taccgttctc atcatccctt agtttcctgt gcgattagcc acaaaccctg
     9601 cagcacgaga ttatccacga ggcggagcgg caggttaagc cgctcgcgca aaacttccgc
     9661 cgcaccgccg ctcaacacga cctcggcccg cgcaacgcca agcaagccat gctgccgctc
     9721 gatcgcgccg caactggcct gcaccacgcc gctatacatc gcatcggcgg tgttgcacgg
     9781 gaatgccgca tacgcgccgg acgccgcctg caactgcgcc gccgcaccgc acaggctgcg
     9841 ctgcatcagc tccacgccgg gcaagatcag gccgccgatg aattctccgt tttcagacaa
     9901 cgcatccacc gtggtggccg tgccgcagtt caccaccaga caacacccgc ccaccagatg
     9961 ccatgcaccg atcaacgccg cccaacggtc actgcccaga cgcgacgctt cggcatagcc
    10021 gttgcgcaca ccgcattgtc gctcgcaggc cacgatgaaa tgcagcttat ccggcgcaac
    10081 gctgttgcat atcgcttgcg caaccgcctc acccgccaca ttgctcaccc acacatcctg
    10141 tatgtcaggc gcatcggcga attgagcaaa cagctcaccg acccgctcta caggcaacac
    10201 cccgctgcgc agccatgcat cgccttgcgt caacgcccac ttgatacgcg tattgcccgc
    10261 atcgatcagc agcctcatca ccgcactccc acttcgccgg aatgaaaact ccgcaacccc
    10321 tgcgcggtct ccagcagcaa ttcaccgacc tcgcccactc cgcgcgcgat tccgctcacc
    10381 acgctcccgt cagccataca cagctgcaca ggacgatcct gatagagatg atagcgctcc
    10441 cactcgccac gaaacgggcc aaagccgtcc cgcccgaact ggctcagcac tgccgacagt
    10501 tccagcagca gcaacgccag caatcgattg cgcggcggca gcgccgcgca caactcgtcc
    10561 agcgcacagg ccggctgatc gatctgccgc accagcgatt ccggcagatg gcagttcagg
    10621 ccgatgccga ccaccaccgc actcggcccc agcatatcgc cctgcgcctc gagcaacacg
    10681 ccgcccagct tgccctgtgc gcacaggatg tcgttcggcc acttcaaccc gacgccctgc
    10741 acaccggcct tgttcagcgc acgcaccacg gccactccga cgaccagact caggccggac
    10801 agggcattca gaccgcagtc gaagcgccat aacagcgaaa aagtcagcgc attgcccaga
    10861 ccggaatgcc aggttcgccc cagacggcca cgcccggcac tttgcaactc tgccgcgacc
    10921 acgctgccgc cgggcgcgcc caatgcggcg cgctgcaaca acagcgtatt gctggaggcc
    10981 gtctgaggca cgatctcgat atcgaatcgc ccgctctcct cgcccagcca gcccgcgacc
    11041 tcatcgcgct gcaggcgctg ccagggctgc gccagccgat aacctcgccc gcgcacgcgc
    11101 tgcaccgtca cgccgcaatc cgccgcgacg gccagtgcat tgaatacgct ggcgcgcgac
    11161 acgccgagct gcccggccag cacctcgccc gaatggaaat gaccgtcggc cagaatatcc
    11221 agcaattgcc aggttctggc gcgcagcccc ccggcactca ttgcgtgtcc cgatctttct
    11281 ggcagagcga ctcacccaaa aagcgcatga tctcctcgcg ctgctgtagg gcatcgctat
    11341 agcccaattc gatcaacgca cggcaatatt tcttttccga caacaggaag ctgatcaacg
    11401 ccgatccgcc actgcgcggc gccgcgccga tcaggcgcaa cagcacccgt atcgtccagg
    11461 gcaattcgga catgtagcgc tcggcgatct tgtcgatgga ctcgctcggc ttgatcacca
    11521 gcagctcgac gtacttcagc ccggtcatct tgcgcatctc ttccggcatg atttttacca
    11581 gttcattgat cgtctgcacc cgctccagat cgacctccat gctatccatg aaaaggctgt
    11641 tgagcgcatg gccggcgatc tgcgccagcg agggatagga tccctccacg gcacggcgcg
    11701 actgctccag cgcatcgttg gtcgcaccga ccaccagaat acgggtcgct cccagattca
    11761 gcgccgaact gatgggggcg atctgccgca tcgagccatc gccgaaatac tcgcggttga
    11821 tcagccgcgc gggaaaaata aaggggatcg cagccgaagc cagcagatgt ttcacttcga
    11881 tatcagtggg cacccccact cgccgcgccc ccttccaggg cttgatgtcg ctcttcccct
    11941 gatagaaagt aaccgactct cccgactcgt aaccggaagc cgtgatgctc agggcatgca
    12001 gcaatccggc atcgatgttg tgctgtatct tttcgcaggg aaggatctcg tccagcaact
    12061 cggccagcgg cgcattatcc agcagcgaca cctgatcgag ccggttgata cccagcccgc
    12121 taagcaccaa tccggccagc cacaatgccg tgttgttgaa caccccgacc acatcggtac
    12181 gatagatctg gtcggtatgg aagttctgcc agatgttgcg cagatagcgc acggaacggc
    12241 ggaagttatg cgcattcacc gccagtgcgg cggcattcag cgcacccgac gaggaaccgc
    12301 agatcaccgg aaaggggtta tgtgcatggc gcggcagaca ctccgcgatc gcctgcaaca
    12361 caccgacctg ataagccgcg cgcgctccgc cgccggtaag aatcaggccg acacgttcac
    12421 ccatgttacc ggagctcgcc ccgtaccgaa tccagcgcgt ccttcagcgt ttcctccgac
    12481 aaggcattga gcaactcgga caacgcatcg gcggcctcta ccgcctcctt gggcaaagcc
    12541 cgcacttcgc tcttcgatac cagcaccgcc gcagcgccca cgaccttgag caggcgcatg
    12601 cgctcgacca tcagcatctc aacttcctga cgcagcagtt cgatttcctg tttgttcatt
    12661 tgcgacccct tcaaaatttt cccgaccgcc atttacaccg gccgaaggat ggcatgcttc
    12721 gccgtataga ctcagtgcgt cttcacgccg accaccgcca gcgcactcat gttgacgatg
    12781 cgacgcacgg tagcagtatt ggtcatgatg tgcaccgatt ttgccgcgcc cagcaacaca
    12841 ggcccgatgg tgatgccgtc gccggccagc accttgagca gattgaaagc gatgttggcc
    12901 gcatccagat tcggcatcac cagcagattg gcgttgtcct tcagacgcga gtccgggaac
    12961 agttgcgcgc ggatcgcggc agacacggcg gcgtcgccgt gtatctcgcc ttccacctcc
    13021 agccccggag cctgctgctc cagcaactcg cgcgcgcggc tcatcttgcg cgccgaggca
    13081 tcgtcgaagc tgccgaagtt ggaatgcgac agcaaggcca ccttgggttc gatgccgaag
    13141 cggcgcactt ccgccgccgc cagctgggtc agttcggcga gctgctcagc ggtcggatcg
    13201 aggttcacat gggtatcgca aatgaacagc gtgtgctcct tcagcatcag gatgttcatc
    13261 gccgccagca ccgatgcgcc ctcgcgcaga ccgatcacct cgcgcacatg acgcagatga
    13321 tcctgcggct gatccgtgac gccgcacagc atcgcatccg cgcggccttc gcttaccagt
    13381 ctggcggcga ccagcgtcgg atgacaagtg tcgcccgcgc agtccaccag ctcgaagtgc
    13441 tcccccgcga ccagacgcaa gccgagcttg tcgatgcgcc gcacgatggt cgccgcatcg
    13501 ccgaccagaa tcggcttcgc cagcttctcg tccaccacgg tgcgcaccgc atgcagcaca
    13561 cgctcgtccg cgccctcggc atagaccagc cgtttcggat cgcgcttggc ggtatcgaac
    13621 accggcttca tcagcagacc ggactgatag acgaagtggg agatctgttc gcgataggcc
    13681 gccatgtcgg cgatggggcg cgtcgccacg ccggaatcca tcgcggcctg cgccacggcg
    13741 ggggcgacgc gcttgatcag gcgctggtcg aaaggcttgg ggatcaggta gttcgcaccg
    13801 aagctctgct cgtcttctcc gtacaccgag gacacggcgt gcgactgctc cgcacaggcc
    13861 agctcggcga tggcatacac cgccgcacgc ttcatttcct cgttgatggt ggtcgccccc
    13921 acgtccagcg cgccccggaa catgtaaggg aaacacagcg cgttgttcac ctggttcgga
    13981 taatccgaac gtccggtcgc gaggatggca tcgggacgca ccgccttggc atgctccggc
    14041 agtatctcgg gattgggatt cgccagcgcg aagatcagcg ggcggtcgcc catctgcaca
    14101 accatctcgg gtttgagcac gccgcccgcc gacagcccga ggaacacgtc ggccccggcg
    14161 atcacttcac ccagcgtgcg cgcagaggtc tccaccgcat aacgcgcctt gttgtcgtcc
    14221 atctcttcgg tacggccctt gtagaccacg cccttgatgt cggtgacggt gatgttcgcc
    14281 atgctcacgc ccaggcccac cagcatatcc agacaggcca gtgcggccgc gcccgcaccg
    14341 ctcaccacca gcttgatctt gccgatgtct ttgcccacga ccttgagacc gttgagcagc
    14401 gccgcaccga ctatgatcgc ggtgccgtgc tggtcgtcgt ggaacaccgg aatcttcatg
    14461 cgctcgcgca gcttgcgctc gatgtagaaa cactccggcg ccttgatgtc ttccagattg
    14521 atgccgccga aggtcggctc cagcgatgcg acgatgtcaa ccagcttgtc cgggtcgttc
    14581 tcggcgacct cgatatcgaa cacatcgatg ccggcgaatt tcttgaacag cacgcccttg
    14641 ccttccatca caggcttgga agccagcgga ccgatgttgc ccagccccag caccgccgtg
    14701 ccgttggaga tcaccgccac cagattgccg cgcgcggtca gattgcgcgc ctcgttcggg
    14761 tcgcgcacga tctcctcaca ggccgccgcc acgccgggcg aataggccag cgccagatcg
    14821 cgctgggtct gcatcggctt gctcggcacg acactgattt ttcccgccgg cgtctggcgg
    14881 tggtattgaa gcgcagcttc gcgaaattgt ttgtccataa tttgcctcga taaaccttaa
    14941 aaatactttg tccgcagagc aaaaaagctc cgcctgtttg atgatgcgac ctgtagaagc
    15001 gattgaaatc tgcgtcaatc tgcagaatct gcggatagca cttcgttttt cagaaaaaac
    15061 gtcagcgcgg attatagcgc agcggcctcc ttcctcttag cactgcctct acacaagaca
    15121 ggtgatgcac gccatctgct agacttcaac gatgtacccg gccctcaccc cgcaacgcga
    15181 acgcttcctg ttactggccc tcgccggcat ccagttcagc catgtgatcg acttcatgat
    15241 catgatgccg ctcggcccga ttctgatgat cgagctgggc atcaccactc acgaattcgg
    15301 cctgctggtc gcgtcctacc gtttcactgc cgcgctgtcc ggtctgctca ccgccatctt
    15361 cgtcgaccgc ttcgagcgaa aacgcatgct gctcacgatg ttcgcgctgt tcggcatcgc
    15421 cacgctggcc tgcgggctgg cgcccggcta cgccacgctg ctggtcgcgc gcggactggc
    15481 gggcgcgttc ggcggcgtga tgggcgcgct gacccagacc atcgtcggcg acgtgatccc
    15541 gttcgcgcgc cgcgcccgag ccggcggcat ggtttcggca tcattctcgg ttgccaccgt
    15601 cgcgggtgtg ccgctgtcgc tgtggctggc caatcacttc cagtggcgcg cccccttcat
    15661 tttcatcgcg atactggtcg tgctgttcat catcgtcggc ctgcgcgtcc tgcccgagct
    15721 gcgcggccac atcgccacgg aaaaacgcgc ccacccgttc gccccgatgt tcgacgtgct
    15781 gcgcgacgca aaccatctgc aagccctgct gttttcggca ctgatcatct tttccggctt
    15841 cacggtgatt ccctacatca ccgtgttcgc ggtcagtaat atcggcatct cgcagcacga
    15901 catcccctac atctacctgt tcggcggcgc cgccacgctg gtcaccgcaa gactgatcgg
    15961 ccactgggcc gacctgtacg gcaaggtcag ggtctatcgc atcgtcgccg ccgccgcgat
    16021 gctgccgctg ctggtcgtga cccatctggg cgaaacgccg ctgtggttct ggataatctg
    16081 caccaccgtc ttcttcgtga tggtatcggg ccgcatgatc ccggcgatgg cgatcatcac
    16141 ctcggcggca cagcccagac tgcgcggcac cttcatgtcg atcaacgcca ccatccagtc
    16201 gctcgcgatg ggtctggcca ccacgctggc cggtttcatc attgcgcagg acgccgatgg
    16261 caagatcgtc cactacagca ccgtgggcta tgtcgccgtc accgccaacc tgctggcgat
    16321 ctggttcgtc ggccgcatcg cgatgcacga ccagcacaaa tagtccgact cgctactttc
    16381 tacttgccac tcgccatcag gttatagaat agcgccccga ccccaaggac ttgccatgac
    16441 ctggaaactc ctcatcaacc gcattcaaca atggcgcgaa catctggccg agcgcgccgc
    16501 gcagttgttg aaatacagtt tctatctcta cctcgccgga ctgttcagcg tgttcgcggt
    16561 gctcgacacg accatgctgc atttcaccag cgagatgcgg caggcggcgt tcgacaccat
    16621 ggtgcgctac cgcatcgtgg tgcccaaacc cgatcccgag atcgtcatcg tggacatcaa
    16681 cgaggccagc ctcgcggcga tggcacagga ttacgggcgc tggccctggc cgcgtcaggt
    16741 gcttggcgag ttcctcgaac agatcgagaa gcagcagccc aaggcggtgg tgttcgacat
    16801 cctgttcagc gatgcggacg tgtacaaccc ggacagcgac acctatttcg aggccgccat
    16861 cgccaacacc cgcaatacct atttcccgat gctgcggctg gaccccgcca gcgactcgct
    16921 gagccagatc aagcccgcga tgatccccgg cgtcaggcct ctgcccggca cgcagccgga
    16981 cgatgcggcg acgatcgccg tggtgctgcc gcacttccag gccgcgctcg acggcggccg
    17041 cctcggcctg cacaacatct atccggacag cgacggggtg gcgcgccgct acaacgtgca
    17101 acgcgacgac tacggctggg cgctgccttc gctgccgacg cgcgtcgcgc gcgaactggg
    17161 ctggcccgag ccggatgcgc aacaggtgct gctgaactgg cgcggccagc cgttcactta
    17221 ccgctacgtc agcttcagcg atgtgtatgc cgacatgggc agcaaggaca agaagcgtcc
    17281 gcaggacgaa ttcaagaaca agatcgtcat catcggctcg accgcgccca gcctgttcga
    17341 catcaaggcc acgccgatga accgcatgtt ccccggcgtc gagattctcg ccaccgccat
    17401 cgacaacttc aagcatggcg acgcgctgcg attccccgag ggacgcatgt ggtatctgct
    17461 gatcacgctg accatcatct ggctgaccgc gtggggcttc taccgcgatg ccgggcgcga
    17521 caagatcgac cgcgtgttcg gcctgtccca attcatcctg atcggcatca cctacgccag
    17581 catcaacttc accaacgtct acatcaacct caccggcccg gtgaccatcg ggctggcgtt
    17641 cttctcgctg gcgcgtctgt atgcgatggc caccggcaag gcgctggagc agaacatggt
    17701 gcgcaccgcc accgcccgca ccggcgaatt gcaggccacg ctgctgctga tccgtctgga
    17761 taacgaacgc aacgcgattc gcccggtcgc gcaggaaaag atacgccacg cgctggaaaa
    17821 gaccggcgca gacaacaaga gcgtggacgt gctgaccggc gaccagaagg ggctatggga
    17881 cctgttcgag aacacgctgg cgatctcctg ggtcgctgaa gcgcataatg cagaggcgca
    17941 ggcagcgatc cggcgcgaca tcgatgcgct gctggccgcg ctgccgggcg tattgcaaca
    18001 acatctggcc cgcccggaca atgcggccag ccacttcatc catcagggcc gcatcgatgg
    18061 cggtgccgct gcggcggcgg gctggcgcgt gatgttcgcc gaggcattgc tggaatggga
    18121 caaaaaggag aacgagcaat catgaaaaaa tcgagattcg gattaacact ggccgccctg
    18181 ctgctcagcg gcgcgttgca tgccgccgaa agcggcaccg cactcaaggc tgacgagatc
    18241 aaggccgaac cgttccgcga tgccaagacg gtggccaaac tggcgaccgg cgacaaggtg
    18301 gacatcctca agaaagatgg cggctggttt caggtgagca gcgcgaaggg ccgtggctgg
    18361 gtgcgcatgc tgagcatccg caagggcgag gcgggcaaag gctcaggcgc cggcggcctg
    18421 ctcgcgatga catccggacg cgccggcacc ggcaaggtcg tctcaaccac cggcatccga
    18481 ggcctgaacg aggaagagct gaaggccgcg aagttcgacg aggcggaatt gaagcgcgcc
    18541 gagtcctatg ctacgaacaa gagcgcggcc gccaagttcg ccgcacaggg caagctcgcc
    18601 gcgcgcccgt tcgattacct gcctgcgcct aaataataag gagagcaacg atgaaccaca
    18661 agacattctg gattctggcg ctggcgctgt tttccgctaa cgcgctggcg gccaatccat
    18721 tcgagcagca actgttcgat gcgctcaagc agaacgtgca ggaaaaagtg accggcaaat
    18781 cggccacttc ggtggaagaa gagaccgcca tcgggcggca gatcgccggc aacctgctgg
    18841 gcgcggcgcc gctggtcaaa gacgcgaagc tgcagaaata cgtcaacaac gtgggccgct
    18901 gggtcgccga ccagagcgag cgccccgacc tggtctggca cttcggcgtg atcgcctcga
    18961 acgacgtgaa cgccttcgcc gcacccggcg gctacatttt cgtcaccaag gggctctaca
    19021 agttgctgca gagcgagtcc gaactggccg gcgtgctggc ccacgaggtc ggccatgtga
    19081 tccgccagca ccatctgaaa atcctgcagc agagcaagct gctcgacctg ggcagccagg
    19141 cgatcgcaca aaatgtcgga ggcaacgacc gcgtgcaggg tctgatcggc agcggcgccg
    19201 agatcgtgtc gcgctcgctg gacaaaggcg ccgaattcga agccgaccgc atcgccgtgg
    19261 tgctcgccac ccgcgcgggc tacgatgcct tcggtctgcc gggcgtgctg caacagatcg
    19321 gccatttcgc caaggacgac aatgatgtcg cgctgctgtt caagacccac ccgcatccgg
    19381 acgaccgact gggcaaactg ggagccgcga tggacgaccg cttcgacaag gtgaagggca
    19441 agacgctgga gaagaggctg tatcgggtca agtagtaatc gagaatgcac caactttctt
    19501 agggagcaat acaaatcatg cgcttcactt tagag
//
LOCUS       DASUIV010000103         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_24296, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000103 DASUIV010000000
VERSION     DASUIV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24296"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2365)
                     /locus_tag="VFW59_03825"
     CDS             complement(<1..2365)
                     /locus_tag="VFW59_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5363371.1"
                     /translation="MQQEYDQMWKRLADRPIESLVDLPLMSDPVWGGTMDVLSSEIAP
                     ATLMDGNLLCLVVARMANLSLEHGNTDGSCQAYVYLGMLLGEQFDNYPAAFQFGKLAI
                     DLMDKHGLERYKGRVSMCFGAFVNPWARHIAGGRRWIQTAFDILNRNGDLTWAGYCGN
                     NLTTNLLASGELLAEVQQEAEHRLQFAQKARFGLVIDMISGQLGLIRALRGMTSALGS
                     FDAADFDEQSFRARLEADSRLAFPAGSYWIRKQQALFAAGEYDAALDAAAQAERYLWT
                     FPSFFEKAEHHFYAGLARAACCLSAPPDDCGRHLAALSVHHRQLAAWAKNCTENFGDR
                     VALLAAEMARIEARELEAQNYYEEAIRAAHENGFVQNEALGYELASGFYRSRGFIKFA
                     DTYLVEARACYARWGADGKVKQLEGLHPWLAESRTPDSLAIASFTEQLDVMAVAKAQR
                     AISGEIVRERLTETLLRIVLESAGAQRGYLLVEPDTELLAEVGADGEVEAHRVSAGAS
                     PMASAAILNYVRRTRETVILADAGSNAGDFASDEYLLQAQPKSVLCMPILRQATLIGV
                     LYLENNLTTGTFTPDRRAVLEILASQAVISLENALVFDALQESESKYRRIIDTANEGI
                     WLLGPDTTILSVNARMAEMFGFRPEEMIGRKGPDFMFDEDMPAHLKRMENRRQGRSEN
                     YEVRFRRRNGETLWTLVSATPIFDEEHRFAGMFGMFTDITERKRAEDEVRMLNLQLEK
                     RVSERTVQLETANQELEAFSYSISHDLRTPLRAIDGFSRQLATRYADQ"
BASE COUNT          478 a          758 c          669 g          476 t
ORIGIN      
        1 gttgatcggc ataccgggtc gccagttggc gcgagaaccc gtcgatggcg cgcagcggcg
       61 tgcgcaggtc gtgcgagatg gaataggaaa aagcttccag ctcctgattg gccgtttcca
      121 actgcacggt acgctcggat accctttttt ccagttgcag gttcagcatc ctgacttcgt
      181 cttccgcgcg cttgcgttca gtgatgtcgg tgaacatccc gaacataccg gcaaagcgat
      241 gctcctcatc gaagataggc gtggcggaaa ccagcgtcca cagcgtttcc ccgttgcgac
      301 ggcgaaaacg tacctcataa ttttcggatc gtccctggcg acggttctcc atgcgcttca
      361 gatgagcggg catatcctcg tcgaacatga agtccggccc ttttcggcct atcatttctt
      421 cgggcctaaa gccaaacatt tcggccatcc tggcattcac cgaaagaatc gtagtatcgg
      481 gcccgagcag ccagattcct tcgttggccg tatcgatgat gcgacgatac ttggactcgc
      541 tttcctgcaa cgcatcgaaa acaagggcat tttccagaga gattaccgcc tgcgaagcca
      601 gtatttccag tacggcgcgc cgatccggcg taaaggtccc cgtagtcaga ttgttctcca
      661 gatagagaac gccaatgagc gtagcctgcc gcaagatagg catgcagagc acggattttg
      721 gttgggcctg tagcaggtac tcatccgagg caaagtcacc ggcattcgag ccggcatcgg
      781 cgagaatcac ggtttcgcgg gtgcgtctga catagttgag aatggctgct gacgccattg
      841 gggaggctcc cgccgagact cgatgggcct cgacctctcc gtcagcgcca acctccgcaa
      901 gcaattcggt atccggctcg acgagcaaat accctcgctg cgcccccgcg ctttccagga
      961 ctatccgcag cagcgtttcc gtcagtcgtt cccgcacgat ctcgcccgag atggcacgct
     1021 gcgccttggc cacggccatc acatccaact gttcggtaaa actggcgata gccaggctgt
     1081 ccggcgttct tgattcggca agccacggat gcaagccctc cagttgcttc accttgccat
     1141 ccgcccccca tcgcgcatag cacgcgcggg cttcgaccag ataggtatcg gcaaacttga
     1201 taaaaccgcg cgaccggtag aatccagacg ccagttcata gcctagcgcc tcgttctgca
     1261 caaagccgtt ttcgtgcgcc gcccgaatcg cctcttcata ataattctgg gcctccagtt
     1321 cgcgcgcctc gatgcgggcc atttcggcag ccagcagcgc aacccgatca ccgaagttct
     1381 cggtgcagtt tttggcccag gccgccagtt gtcgatggtg gacgctcaac gccgccaaat
     1441 gtcgcccgca gtcgtccggc ggcgcagaca ggcagcatgc cgcccgcgcc aggccggcat
     1501 aaaaatggtg ttccgctttt tcaaagaacg aaggaaacgt ccagaggtag cgctccgcct
     1561 gggctgctgc gtccagcgcg gcgtcatatt cgccggcggc gaacagggct tgctgcttgc
     1621 gtatccaata cgagccggcc ggaaaagcca agcgggaatc ggcttcaaga cgcgccctga
     1681 acgattgttc gtcgaaatcg gcggcatcga acgagcccag cgccgacgtc atgccacgca
     1741 gcgcccggat cagcccgagc tgtccggaaa tcatgtcgat gaccaggccg aaacgcgcct
     1801 tctgtgcgaa ttgcagcctg tgctcggcct cctgctgcac ttccgcgagc agctccccgc
     1861 tggcgagaag attggtggtc aggttgttgc cgcagtatcc ggcccaggtg agatcgccat
     1921 tccggttaag gatgtcgaac gcggtctgta tccatcggcg accgccggcg atgtgcctgg
     1981 cccagggatt gacgaaagcg ccgaagcaca tgctgacccg ccccttatag cgctccagcc
     2041 catgcttatc catgaggtcg atagccagct tgccgaattg gaatgccgcc ggatagttgt
     2101 cgaactgctc gccaagcagc atccccagat agacatacgc ctggcaggag ccgtccgtgt
     2161 tgccgtgctc caggctcaag ttcgccatcc gggcaaccac gagacacagc agattgccgt
     2221 ccatgagcgt cgccggggca atctccgagg aaagcacgtc catcgtcccc ccccacacgg
     2281 ggtcgctcat caagggcaaa tccaccagcg actcgatggg gcgatccgcc agccgtttcc
     2341 acatctggtc gtactcttgt tgcacctctt ctcgcgtggg a
//
LOCUS       DASUIV010000104         7613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_24331, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000104 DASUIV010000000
VERSION     DASUIV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24331"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /gene="mutS"
                     /locus_tag="VFW59_03830"
     CDS             complement(<1..1813)
                     /gene="mutS"
                     /locus_tag="VFW59_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5363372.1"
                     /translation="MTQTDTQKPAAEKHTPMMQQYLRIKAEHPDKLLFYRMGDFYELF
                     HEDAARAAKLLDITLTQRGASNGNPIKMAGVPYHAAEQYLARLVKMGESIAICEQIGD
                     PATSKGPVERKVMRIITPGTLTDSALLDEKRDNLLLALHENRGMLGLAWLNLASGQFH
                     VCETAPENLAAELERLQPSEILHAENARLETGGRAALKSLPDWHFDLETARRALCQQF
                     ATLDLAGFGCDDFSAGLEAAGALLGYARLTQGQSIAHIRSMQVYSADRYVRMDAATRR
                     NLEITQTLRGEPAPTLLSLLDTCATNMGSRLMANWLHHPLRDRAVLRARLDAVEQLQL
                     PSPAGGRGAGGEGYRAIHDQLKPSVDVERITARIALKSARPRDLSGLRDTLLTLPQLH
                     ATLSACESPLIDSLADALQADIPLVERLQRMLKAEPSSVLREGGVIADGFDAELDELR
                     GIQNNCGEFLLALEAREKSRSGIATLKVEYNRVHGFYIEVSTAQSANVPDDYRRRQTL
                     KNAERYITPELKAFEDKALSANDRALAREKMLYDQLLDQLAPGIPQLQRIAAALAELD
                     VLASFAERASTLNFCAPQFSDDAQISITQGRHPVVEAQ"
     gene            complement(1839..2480)
                     /locus_tag="VFW59_03835"
     CDS             complement(1839..2480)
                     /locus_tag="VFW59_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX5363373.1"
                     /translation="MTEASKTLLAIILDRVFPKMPDFFTMLTEQTVQVARTVDLLVEY
                     MEHDGPAVADALKLDVHEADMLKVRNLHELNDAFSTPIDREDIYRAIMSLDDIVMYCK
                     TTVHEMDVLGVKPDKFMLDISHFIKEGVDALATGYALLGSKPADAAQYADAARKADRR
                     AEKQYRIALSTLFEGSDYVNMFKRREIYRHLTNAAQHMAQCANTLHDIVVKIT"
     gene            complement(2503..3534)
                     /locus_tag="VFW59_03840"
     CDS             complement(2503..3534)
                     /locus_tag="VFW59_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX5363374.1"
                     /translation="MDTLLILAIATIVVVLLFEYTNGFHDAANIIATVIASRAMTPVQ
                     AVLVVAFFEFLGPLLGGTAVANTIGKFITLDDLDRVTALTIVLCGLLGAIAWNLLTWW
                     RGIPSSSSHALVGGLVGVVVVAAGGSHVSWGFSELLHGHLTGVAKLLVALILSPVIGF
                     WVGFALHRTMLWLLRGARPSINRDLRHLQFVTSAGLAFSHGANDAQKSMGILTLVLLL
                     GGFIPSFEVPLWVMLACSISITLGILSGGWQIVRTLGFSIYKIRPLHALDSQLTAASV
                     IFASSLAGAPVSTTHVVASSIMGIGASERPKAVRWGKAREIVTTWVITIPGAALMSIG
                     CYFAVQLFV"
     gene            complement(3546..4346)
                     /locus_tag="VFW59_03845"
     CDS             complement(3546..4346)
                     /locus_tag="VFW59_03845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5363375.1"
                     /translation="MHPMLNIAVKAARRAGNLIYRSADKLDHLTVTKKSHGDFVSEVD
                     RAAEQTIIQTLLEAYPTHAILAEESGSQGESEFLWIIDPLDGTTNFLHGFPQFAVSIA
                     LQHKGIVTQAVVYDPVKNELFTATRGRGAFLNDKRIRVTKRVHMADALIGTGFPYTKF
                     EHMDAYIGIFKDLMQQTSGLRRPGSAALDLAWMAAGRYDGFFETGLKPWDIAAGTLLI
                     TEAGGMVSDLHGSDTFLKTGHICAGNPDIHPQLLKVIEPHLTPALKGQ"
     gene            4430..5203
                     /locus_tag="VFW59_03850"
     CDS             4430..5203
                     /locus_tag="VFW59_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5363376.1"
                     /translation="MNKQNPLENIRVVLSHTSHPGNIGAAARAMKTMGLRHLYLINPY
                     RFPDPQAVAMAAGADDILESAVVCGSIDEALQGVAFTVAMTARLRDISIEVKTPREAV
                     PELLQQAASQPVALLFGTEMSGLTNEEMGRAQVLANIPANPDYSSLNLAAAVQVMAYE
                     LNVAAQAFVPALPEVLPATHEQMEGLYAHLEKTLFAIGFFTTQNPARMMQRLRRLFSR
                     ARPEPEEINILRGILSVTTEYNARLKNRYLEEHSDVQEH"
     gene            5187..5915
                     /gene="cysE"
                     /locus_tag="VFW59_03855"
     CDS             5187..5915
                     /gene="cysE"
                     /locus_tag="VFW59_03855"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX5363377.1"
                     /translation="MFRNIREDIASVFDRDPAARSTFEVLTCYPGLHARMLHRLSNKL
                     WHARLKWPARFLSHLTRWFTGIEIHPGATIGRRFFIDHGMGVVIGETAEIGDDVTLYH
                     GVTLGGTSWKEGKRHPTLGNGVVVGAGAKVLGPIHIGAGAKIGSNAVVVKDVPAGATA
                     AGIPARILDEEKKKTGFNAYGIGNDQNDPVTKALHGLLGHSVTVDQRIDFILQQLEKM
                     GVPVDEERATADKFDPNHLNKIVD"
     gene            6011..6481
                     /gene="iscR"
                     /locus_tag="VFW59_03860"
     CDS             6011..6481
                     /gene="iscR"
                     /locus_tag="VFW59_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEX5363378.1"
                     /translation="MRLTTKGRFAVTAMVDLAMRGSKGPVTLASISERQGISLSYLEQ
                     LFGRLRRNNLVESVRGPGGGYYLARGADKLSVAEIILAVDEPLDATSCGGKGDCHDNK
                     PCITHDLWMGLNEKIFDYLESVNLQQLVEGHNKLNGGAVPILMDRSHKAPSAAV"
     gene            6512..>7613
                     /locus_tag="VFW59_03865"
     CDS             6512..>7613
                     /locus_tag="VFW59_03865"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX5363379.1"
                     /translation="MDYSATTPVDPRVAALMIPYLTEKFGNPASRSHSFGWEADAAVE
                     QAREQVAALVNADPKEIVWTSGATESNNLAIKGAAHFYQGKGKHIVTMRIEHKAVLDT
                     VRELEREGYSATYLDPEPSGLLDLEKFKASLQADTVLVSIMLVNNEIGVIQDIVAIGD
                     ICREKGILFHVDAAQATGKVEIDLQQLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRL
                     EAQMHGGGHERGMRSGTLATHQIVGMGEAFRIAKEEMAQENERVRMLRDRLLDGLKDM
                     EEVHVNGDMVHRVPHNLNLSFNFVEGESLIMAISDIAVSSGSACTSASLEPSYVLRAL
                     GRSDELAHSSIRFSIGRFTTVEEVDYVIELLKS"
BASE COUNT         1382 a         2216 c         2571 g         1444 t
ORIGIN      
        1 tctgcgcctc caccaccgga tggcggccct gcgtgatgct gatctgcgcg tcgtcgctga
       61 actgcggtgc gcagaaattc agcgtcgatg cgcgctccgc gaagctcgcc agcacgtcga
      121 gttcggcgag ggcggcggcg atgcgctgca gctgcgggat gccgggtgcg agctggtcga
      181 gcagctggtc gtagagcatc ttctcgcgtg ccagtgcgcg gtcgttggcc gacagcgcct
      241 tgtcctcgaa ggccttcagt tccggcgtga tgtagcgctc ggcgttcttc agcgtctggc
      301 gtcggcggta atcgtccggc acgttggcgg attgtgcggt cgacacctcg atgtagaaac
      361 cgtgcacgcg gttgtattcc accttcaggg tggcgatgcc gctgcggctc ttctcgcgcg
      421 cttccagcgc cagcaggaat tcgccgcagt tgttctggat gccgcgcaac tcgtcgagtt
      481 cggcgtcgaa gccatcggcg atcacgccgc cttcgcgcag cacgctggag ggctcggcct
      541 tcagcatgcg ctgcaaccgt tccaccagcg ggatgtcggc ttgcagcgcg tcggccagac
      601 tgtcgatcag cggcgactcg caggcgctca gcgtcgcgtg cagttgcggc agggtcagca
      661 gcgtgtcgcg caggccggac aggtcgcgcg gacgcgcgct cttcagcgcg atgcgcgcgg
      721 tgatgcgctc cacgtcgacg ctgggtttga gctggtcatg aatggcgcgg taaccctctc
      781 ccccagcccc tctcccgcca gcgggagagg ggagttggag ttgttcgacc gcatccagcc
      841 ttgcgcgcag cacagcgcgg tcgcgcagcg ggtggtgcag ccagttcgcc atcaggcggc
      901 tgcccatgtt ggtcgcgcag gtgtcgagca gcgacagcag cgtcggcgcg ggttcgccgc
      961 gcagcgtctg ggtgatctcc aggttgcggc gggtcgctgc gtccatgcgc acgtagcggt
     1021 cggcgctgta gacctgcatg ctgcggatgt gggcgatgct ctggccctgc gtgagtctgg
     1081 cgtaacccag cagcgcgccc gccgcttcaa ggcccgcgct gaaatcgtcg cagccgaagc
     1141 cggccagatc gagcgtggcg aactgctggc acagcgcgcg ccgcgcggtc tccagatcga
     1201 agtgccagtc gggcagcgat ttcagcgctg cgcggccgcc ggtttccagt ctggcgttct
     1261 cggcgtgcag gatctcggaa ggctgcaggc gctccagttc ggcggccaga ttttccggtg
     1321 cggtctcgca gacgtggaat tgtcccgagg ccagattcag ccaggccagc cccagcatgc
     1381 cgcgattttc gtgcagcgcc agcagcaggt tgtcgcgctt ctcgtccagc agcgcggagt
     1441 cggtcagcgt gccgggggtg atgatgcgca tcaccttgcg ttcgaccggc cccttgctgg
     1501 tggcgggatc gccgatctgt tcgcagatgg cgatggattc gcccatcttc accagtctgg
     1561 ccagatattg ttcggcggcg tgatagggca cgcccgccat cttgatcggg ttgccgttgg
     1621 aggcgccgcg ctgggtcagc gtgatgtcca gcagcttggc ggcgcgtgcg gcgtcctcat
     1681 ggaacagctc gtagaagtcg cccatgcggt agaacagcag cttgtcggga tgctccgcct
     1741 tgatgcgcag gtattgctgc atcatcggcg tgtgcttctc ggctgcgggt ttctgggtgt
     1801 ctgtctgagt catgggctaa ttcgggtgcg agggtttttc aggtgatctt gaccacgatg
     1861 tcgtgcagcg tgttggcgca ttgcgccatg tgctgggcgg cgttggtcag gtggcggtag
     1921 atctcgcggc gcttgaacat gttcacgtaa tcggagcctt cgaacagggt ggacagggcg
     1981 atgcggtatt gcttctcggc gcggcgatcg gccttgcgcg cggcatcggc atattgcgcc
     2041 gcgtcggcgg gtttgctgcc cagcagggcg tagcccgtgg ccagcgcgtc cacgccctcc
     2101 ttgatgaagt gcgagatgtc gagcatgaac ttgtcgggtt tgacgccgag tacgtccatc
     2161 tcgtgtaccg tggtcttgca gtacatcacg atgtcgtcga ggctcatgat ggcgcggtag
     2221 atgtcctcgc ggtcgatggg cgtggagaac gcgtcgttca gctcgtgcag gttgcgcacc
     2281 ttgagcatgt ccgcctcgtg cacgtccagc ttgagcgcgt cggccacggc cgggccgtcg
     2341 tgctccatgt attcgaccag caggtccacg gtgcgcgcga cctgaacggt ctgttcggtc
     2401 agcatcgtga agaagtcggg catcttggga aagacccggt caaggatgat cgcgagcagg
     2461 gtcttggagg cttcggtcat ggttgttctc cctaatgagt gctcagacga ataattggac
     2521 ggcgaagtaa cagcctatcg acatcagggc cgcgccgggg atggtgatga cccaggtggt
     2581 gacgatctcg cgcgccttgc cccagcgcac cgccttcgga cgctccgacg cgccgatgcc
     2641 catgatggac gaggccacca cgtgcgtggt ggagaccggc gcacccgcca gcgacgaggc
     2701 gaagatcacg ctggcggcgg tcaattgcga atccagcgcg tgcagcgggc ggatcttgta
     2761 gatcgagaag cccagcgtgc gcacgatctg ccagccgccg gagaggatgc ccagcgtgat
     2821 ggagatcgag caggccagca tcacccagag cggcacctcg aagctgggaa tgaatccgcc
     2881 cagcagcagc accagcgtca ggatacccat gctcttctgc gcgtcattgg cgccgtgcga
     2941 gaaggccaag cctgccgagg tgacgaattg cagatggcgc aggtcgcggt tgatcgaagg
     3001 gcgtgcgccg cgcagcagcc atagcatggt gcggtgcagt gcgaagccga cccagaaacc
     3061 gatgacaggc gacaggatca gcgcgaccaa taatttggcc acgccggtca gatggccgtg
     3121 cagcagttcg gaaaatcccc aggagacatg gctgccgcct gcggcgacta ccaccacgcc
     3181 gaccagcccg ccgaccagcg cgtgcgaaga cgaggagggg atgccgcgcc accaggtcag
     3241 caggttccat gcgatcgcgc cgagcaggcc gcacagcacg atggtcagcg ccgtcacccg
     3301 gtcgagatcg tccagcgtga tgaattttcc aatggtgttg gccaccgccg tgccgcctag
     3361 caatggcccc aggaattcga agaaggcgac caccagcacc gcctgcacag gggtcatcgc
     3421 ccgcgaggcg atgaccgtgg cgatgatgtt ggcggcgtca tggaagccgt tggtgtactc
     3481 aaacagcagc accacgacga tggtcgcgat ggccaggatg aggagggtgt ccatgtcgct
     3541 gccggttact ggcctttcag ggccggcgtc agatgcggct cgatcacctt cagcaattgc
     3601 ggatggatat ccgggttgcc cgcgcagatg tggccggtct tgaggaaagt gtcggaacca
     3661 tgcaggtcgc tgaccatgcc gcccgcttcg gtgatcagca gcgtgcctgc ggcgatgtcc
     3721 cagggcttca ggccggtctc gaagaagccg tcgtagcgtc cggcggccat ccaggccaga
     3781 tccagcgcgg ccgaaccggg gcggcgcagg ccggaagtct gctgcatcag gtctttgaag
     3841 atgccgatgt aggcgtccat gtgctcgaac ttggtatagg gaaagccggt gccgatcagg
     3901 gcgtcggcca tgtgcacgcg cttggtgacg cggatgcgct tgtcgttgag gaatgcgccg
     3961 cgtccgcgtg tcgcggtgaa cagctcgttc ttgaccgggt cgtacaccac ggcctgagtg
     4021 acgatgcctt tatgctgcag cgcgatggac acggcgaatt gcgggaagcc gtgcaggaaa
     4081 ttggtcgtcc catccagcgg atcgatgatc cagaggaact ccgactcccc ttgggagccg
     4141 ctctcttctg ctagaatcgc atgggtcgga taggcttcca gcagggtctg gatgatcgtc
     4201 tgctcggcgg cacgatccac ttcgctgacg aaatcgccat gagatttctt ggtgacggtg
     4261 agatggtcga gcttgtcggc ggagcggtag atcaggtttc cggcgcgacg tgcggctttc
     4321 accgcgatgt tgagcatggg atgcatgggt aacgaccttt ttaatgagct gaagaagtcg
     4381 ggaacgcaaa gttggttccc acggcgcgca ttttagttgg aatcacgctt tgaataaaca
     4441 gaatccttta gaaaacatcc gggtcgtcct cagccacacc tcgcaccccg gcaatatcgg
     4501 tgctgcggcg cgggcgatga agaccatggg cttgcgccat ctgtacctga tcaatcccta
     4561 tcggttcccc gacccgcagg ccgttgcgat ggcggcgggg gcggacgata tcctggagag
     4621 cgccgtggtg tgcggctcga tcgacgaggc gctgcagggc gtggcgttca ccgtggcgat
     4681 gacggcgcgg ttgcgcgata tctccatcga ggtgaagacg ccgcgcgagg cagtgcccga
     4741 attgctgcaa caggccgcca gccagccggt ggcgctgctg ttcggcaccg agatgtccgg
     4801 tctgaccaac gaggagatgg gccgggcaca ggtgctggcc aacattcccg cgaatccgga
     4861 ttactcttcg ctgaacctcg ccgctgcggt gcaggtgatg gcctatgagc tgaacgtcgc
     4921 ggcacaggcc ttcgtgcccg cgttgcccga ggtgctgccc gcgacccacg aacagatgga
     4981 agggctctac gcgcatctgg agaagacgct gttcgcgatc ggtttcttca ccacgcagaa
     5041 cccggcgcgc atgatgcagc gcctgcgccg cctgttttcc cgtgcgcggc ccgagccgga
     5101 agagatcaat atcctgcgcg gcatcctgag cgtgacaacc gagtacaatg cgcgcctcaa
     5161 aaatcgttat ctggaagagc actcggatgt tcaggaacat tagagaagac atcgccagcg
     5221 tattcgaccg cgatccggcg gcgcgcagca cctttgaggt gctgacctgc tatcccggac
     5281 tgcatgcgcg catgctgcat cgcctgtcga acaagttgtg gcacgccagg ctgaagtggc
     5341 cggcgcgctt cctgtcgcat ctcacgcgct ggttcaccgg catcgagatc catccggggg
     5401 cgaccatcgg acggcgcttt ttcatcgatc acggcatggg cgtggtgatc ggcgagaccg
     5461 ccgagatcgg cgacgatgtg accctgtatc acggcgtgac gctgggcggc acctcgtgga
     5521 aggaaggcaa gcgccatccg acgctgggca acggcgtggt ggtcggtgcg ggcgccaagg
     5581 tgctggggcc gatccacatc ggcgcgggag cgaagatcgg ctctaacgcg gtggtggtga
     5641 aggacgtgcc tgcgggtgcg accgctgcgg gcattccggc gcgcatcctc gacgaggaga
     5701 agaaaaagac gggtttcaat gcctatggta tcggcaacga ccagaacgac ccggtcacca
     5761 aggcattgca cggtttgctg gggcacagcg tgacggtgga ccagcgtatc gacttcatcc
     5821 tgcagcagct ggaaaagatg ggcgtgccgg tggatgaaga gcgcgctaca gccgataaat
     5881 tcgaccccaa tcatctgaac aaaatagttg actagaaaat tcgggtattc tactattctt
     5941 cccagtgaga ctgaacggat gtcgcaggac atccagcctg agaacgttat ccgaatttga
     6001 ggggattgag atgcgattaa caaccaaggg gcgttttgcc gtgaccgcga tggtcgatct
     6061 ggcgatgcgc ggcagcaagg ggccggtgac attggcttcg atcagcgagc gtcagggtat
     6121 ttcgctgtct tatctggagc agctgttcgg caggttgcgc cgcaacaatc tcgtcgagag
     6181 cgtgcgcggt cccggtggcg gttattatct ggcgcgcggg gcggacaaac tctcggtcgc
     6241 cgagatcatt ctggccgtgg acgagccgct ggatgccacc agttgcggag gcaaagggga
     6301 ttgtcacgat aacaagccct gcatcaccca cgatctgtgg atgggactga acgagaagat
     6361 tttcgactat ctggaatcgg tcaatttgca gcagttggtc gaggggcaca ataagctgaa
     6421 tggcggcgca gtcccgatcc tgatggacag gagtcataaa gcgcccagtg ccgcagtctg
     6481 aagggagcat gcagtgaaat tacctattta tatggattac tccgcgacca cgccggtcga
     6541 tccgcgcgtg gccgcgttga tgatccctta tctcaccgag aagttcggca acccggcttc
     6601 gcgttcccac tcgtttggct gggaagccga tgcggcggtc gagcaggcgc gcgagcaggt
     6661 cgccgcgctg gtgaatgccg accccaagga aatcgtctgg acctcgggtg cgaccgaatc
     6721 gaacaatctc gcgatcaagg gagccgcgca cttctatcag ggcaagggca agcatatcgt
     6781 caccatgcgc atcgagcaca aggcggtgct ggacaccgtg cgtgagctgg agcgcgaggg
     6841 ctacagcgcg acctacctcg atcccgagcc tagcggtctg ctcgatctgg aaaagttcaa
     6901 ggcgagcctg caagctgaca ccgtgctggt gtccatcatg ctggtgaata acgagatcgg
     6961 tgtgattcag gatatcgtcg ccatcggcga tatctgccgc gagaagggca tcctgttcca
     7021 cgtcgatgcg gcacaggcga ccggcaaggt ggagatcgac ctgcaacagc tcaaggtgga
     7081 tctgatgtcg ttctccgccc acaagactta cggacccaag ggcatcggtg cgctgtacgt
     7141 gcgccgcaag ccgcgcgtgc gactggaagc acagatgcac ggcggtggtc atgagcgcgg
     7201 catgcgctcg ggcacgctgg cgacgcatca gatcgtcggc atgggcgagg cgttccgcat
     7261 cgcgaaagaa gagatggcgc aggagaacga gcgcgtgcgc atgctgcgcg accgtctgct
     7321 ggatggcctg aaggacatgg aagaggtgca tgtcaacggt gacatggtgc atcgtgtgcc
     7381 gcacaacctg aacctcagct tcaacttcgt cgaaggcgag tcgttgatca tggcgatcag
     7441 cgatatcgcg gtatccagtg gttcggcctg cacctcggcg agtctggaac cgtcttatgt
     7501 cctgcgcgca ttgggccgca gcgacgaact ggcgcatagc tcgatccgct tcagtatcgg
     7561 tcgattcacg accgtcgagg aagtggacta tgtcattgag ctgctgaaga gca
//
LOCUS       DASUIV010000105         4977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_24544, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000105 DASUIV010000000
VERSION     DASUIV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24544"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..990
                     /gene="rsxA"
                     /locus_tag="VFW59_03870"
     CDS             409..990
                     /gene="rsxA"
                     /locus_tag="VFW59_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEX5363380.1"
                     /translation="MSEYLLLLLGTALVNNVVLVKFLGLCPFMGVSKKVDGALGMGMA
                     TTFVLTLTTAATWILEHWLLQPLGIQYLRILAFILTIAAVVQFTEMVIRKVSPGLYQV
                     LGIYLPLITTNCAVLGIALLNVQEKNGFMHSLLYGFGSAVGFTLVMLLFTGLRMRLAL
                     AQVPPAFSGAPIGFVLAGLLSLAFMGFAGLAGH"
     gene            1004..1540
                     /locus_tag="VFW59_03875"
     CDS             1004..1540
                     /locus_tag="VFW59_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEX5363381.1"
                     /translation="MMFAAIISLTLLGTILGLLLGVAARYLKVEGNPLVAEIEKLLPG
                     SQCGQCGYPGCSGAAQALAEGTARISLCPPGGRAVMLALADKLGIEADLAGVKESVPM
                     IAEVKEEICIGCTRCFKVCPTDAIMGAAQQVHSVFREACTACGKCEEVCPTESVKLQP
                     IPVTLQSWHWPKPKEVAV"
     gene            1537..3090
                     /gene="rsxC"
                     /locus_tag="VFW59_03880"
     CDS             1537..3090
                     /gene="rsxC"
                     /locus_tag="VFW59_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEX5363382.1"
                     /translation="MTTRRETTCCGAGLPSGNAVATLFGRFFPRLRGGVHPQGRKELA
                     ADCPIRVLPLPKTFFLPLQQHIGAPAKPVVNIGDHVLKGQLIAAAQGAVSSSIHAPTS
                     GVVTALGDNPAPHPSGLPVPTITLESDGEERWIETETLDDPFALSEEEIAARVAAAGI
                     VGLGGATFPAALKLSLSRGVQTLIINGGECEPYLTCDDRIMRERAARIVEGIRLIAYA
                     VGAKEVLVGIEDNKQEAIQAMRSAARNSAVKVVEMPSMYPMGSEKQIIQVLTGREIPA
                     GGRPADIGVLVHNVATAYAVQQAIRFGRPLISRIVTVSGGAIRVPCNVEALIGTPVQA
                     LIELAGGYAQPAARLVLGGPMMGQHFVNTAVPVVKGTSGVLALTSAEIGQDAASPCIR
                     CSTCVRACPVGLVPLEMASRIRAGDLSGAVDLGLKDCIACGSCSYVCPAHIPLVHYFN
                     YAKGELAAQERSKLKQEATKKLAEARGERIARIERERAEAMAKRKAAREAKERAARAA
                     ADKAETETA"
     gene            3087..4145
                     /locus_tag="VFW59_03885"
     CDS             3087..4145
                     /locus_tag="VFW59_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEX5363383.1"
                     /translation="MNQIAHSPHAHAPVTISKVMGTVMLALAPATLFGFWVFGWPAVN
                     LWAVSLIAAILGEAFVLRLQQRAVRPALMDGSGILTAWLLALSLPPFAPWWIAVLGSL
                     FAVIIGKQLFGGLGQNVFNPAMAARVMLLISFPLEMTSWVAPQGASGPGLLESLRVTF
                     FSQPLDGIASASLLGHVKTEFTRGIPLDQALSGYYAPLDALWGSRAGSLGETAALFLL
                     AGGLFLIARRIITWHAPVAMLLGVVVPALLFNAIDGNHYAGPLVHLLSGGLMLGAFFI
                     VTDPVTSPNTALGQFLFGLGCGLLTWIIRTWGNYPEGVAFAVMLMNAATPLIDQLIKP
                     RIYGRDRKGAALPVKKGS"
     gene            4147..4776
                     /gene="rsxG"
                     /locus_tag="VFW59_03890"
     CDS             4147..4776
                     /gene="rsxG"
                     /locus_tag="VFW59_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HEX5363384.1"
                     /translation="MKLDELRGKLGYQGLLLGGAALLTSAALAFASRATAPDIQAAEA
                     ADLKQSLTQVLTGEYDNDLLKDTVLLSGAAGEVTVYRARRAGRVEAVVYRVVGQGYAG
                     SIQCVMGVSREGKIMGVRVIKHAETPGLGDKIEPAKSSWIHDFEGKKLGEPNADKWAV
                     KKDGGVFDQFAGATITPRGVVRAVKGGLAFFAEQREKMLDESSGKGAQP"
     gene            4773..>4977
                     /locus_tag="VFW59_03895"
     CDS             4773..>4977
                     /locus_tag="VFW59_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HEX5363385.1"
                     /translation="MNEKYARITRDGLWDNNVVFAQMLALCPTLAVTSSATNGLGMGL
                     ATTAVLVVSNVIISMVRELVSAEV"
BASE COUNT          852 a         1452 c         1699 g          974 t
ORIGIN      
        1 gcaccattgg catacaggat gttggcgcgt gcatcggtga tggagatcgc gagcgctgcc
       61 tgatccacgg cctgacggaa gatgtgcgga ggtacatgac tgtccggggc gggcttgttc
      121 gatgcttgcg gtgcgggagg cttggccatg atggttccat tcgtgatgcg gatgcgttcc
      181 tacctgcaat aactgtgccc agggttgctc gccggggtgc gatgcctgtt atgacgggcg
      241 ttttgtcggg tttgtgacat ccctgtcggc tatcacccga cagtgacaaa ggattttgtg
      301 cttcagtttg gtgcgtgcac ctgtcaacag gccgagcgcg tgcaactgtg tcggtcgccc
      361 gttctctggc acgaggtttg cgagagaggg ggcaggagat caaacgagat gagcgaatat
      421 ctgttgttgc tgctgggcac ggcactggtg aacaacgtgg tgctggtgaa gtttctgggg
      481 ctgtgcccgt tcatgggcgt atccaagaaa gtcgacggtg cgctgggcat gggcatggcg
      541 acgaccttcg tgctgacgct gacgaccgct gcgacctgga tactggagca ctggctgctg
      601 cagccgctgg gtatccagta tctgcgcatc ctcgccttca ttctcaccat cgcggccgtg
      661 gtgcagttca ccgagatggt gatccgcaag gtcagccccg ggctgtatca ggtgctgggt
      721 atctatctgc cgctgatcac gaccaattgc gcggtgctcg gcattgcgct gctcaatgtg
      781 caggagaaga acggcttcat gcacagcctg ctgtacggtt tcggttcggc ggttggcttc
      841 acgctggtga tgctgctgtt cactggcctg cgcatgcgtc tcgcactggc gcaggttccg
      901 cccgctttca gcggcgcgcc catcggtttt gtgctggccg gactgttgtc gttggcattc
      961 atggggttcg ccggcctggc cggacattaa gggagaagtc gacatgatgt ttgcagcgat
     1021 aatcagcctg actctgctcg gcaccattct ggggttgctg ctcggggtcg ccgcgcgtta
     1081 cctgaaagta gagggtaatc cgctggtggc cgagatcgaa aagctgctgc ccggttctca
     1141 atgcggacaa tgcggctatc cgggttgcag cggcgcggct caggcgctgg cagaaggtac
     1201 cgcccgtatt tccctgtgcc cgccgggagg tcgcgcggtg atgttggcgc tggcagacaa
     1261 gctcggtatc gaggccgatc ttgccggcgt gaaggaaagc gtgccgatga tcgccgaggt
     1321 gaaagaggaa atctgtatcg gctgtacgcg ttgtttcaag gtgtgcccca ccgacgccat
     1381 catgggtgcc gcacagcagg ttcattcggt gtttcgcgaa gcctgcaccg cctgcggcaa
     1441 gtgcgaggag gtctgtccga ccgagtcggt gaagctgcag cctatcccgg tgacgctgca
     1501 atcctggcac tggcccaagc ctaaagaggt ggcagtatga caacacggcg cgaaacgact
     1561 tgttgcggcg caggcttacc ttcaggtaat gccgtagcca cactgttcgg caggtttttc
     1621 ccccgcttgc gcggcggcgt ccatccgcag gggcgcaagg agctggctgc ggattgcccc
     1681 attcgtgtgc tgccgctgcc aaaaacattc tttctgccgt tgcagcagca tatcggcgca
     1741 ccggccaagc ccgtggtgaa tatcggcgac catgtgctga aaggacagct catcgctgcg
     1801 gcgcagggcg ccgtctcttc ctccatccat gcgccgactt ccggtgtggt caccgcgctc
     1861 ggcgacaatc ctgcaccgca tccttccggc ctgccggtac ccaccatcac gctggagagc
     1921 gacggcgagg agcgctggat agagaccgaa acgctcgatg acccgttcgc attgagcgag
     1981 gaagagatcg ccgcacgggt cgctgcggcg ggcatcgtcg gcctgggcgg ggcgaccttc
     2041 ccggctgcgc tcaagctgag cctcagccgc ggcgtgcaga cgctgatcat caacggcggc
     2101 gaatgcgaac cctatctcac ctgcgacgac cgcatcatgc gcgaacgcgc tgcgaggatc
     2161 gtcgagggca tccggctgat cgcctatgcc gtcggtgcca aagaggtgct ggtcggcatc
     2221 gaggataaca agcaagaggc catacaggcg atgcgcagcg cggcccgcaa cagtgcggtc
     2281 aaggtcgtgg agatgccgag catgtacccg atgggctcgg agaaacagat catccaggtg
     2341 ctcacgggcc gcgagatccc cgcaggcggt cgtcccgccg acatcggcgt gctggtgcat
     2401 aacgtcgcga ccgcgtatgc ggtgcagcag gcgatccgtt tcggcaggcc gctgatctcg
     2461 cgcatcgtca ccgtgagcgg cggcgcgatc agggtgccgt gcaatgtcga ggcgctgatc
     2521 ggcacacccg ttcaggcgtt gatcgagctg gccggtggct atgcgcagcc agccgcgagg
     2581 ttggtgctgg gcggcccgat gatggggcag catttcgtca ataccgccgt gccggtggtg
     2641 aagggcacca gcggcgtgct ggccttgacc tcagccgaga tcggacagga cgctgcctcg
     2701 ccgtgcatcc gttgttcgac ctgcgtgcgc gcctgtccgg tcgggctggt gccgctggag
     2761 atggcctcgc gtatccgggc gggcgacctg tccggtgcgg tcgatctcgg gctcaaggat
     2821 tgcatcgcct gcggctcctg ctcctatgtc tgtccggcgc atatcccgct ggtccattac
     2881 ttcaactatg ccaagggcga gctcgccgca caggaacgca gcaagctgaa acaggaagcc
     2941 accaaaaaac tggccgaggc acgcggcgag cgcatcgcgc gcatcgaacg cgagcgtgcc
     3001 gaggcgatgg cgaagcgcaa ggcggcgcgt gaagcgaaag aacgtgcggc aagagcagcg
     3061 gccgacaagg cagaaacgga gacagcatga accagatcgc ccattccccg catgcgcatg
     3121 caccggtcac catcagcaag gtgatgggaa cggtgatgct cgccctcgcg cccgccaccc
     3181 tgttcggctt ctgggtgttc ggctggcccg ccgtcaacct gtgggcggtg tcgctgatcg
     3241 ccgccatcct cggcgaggcc ttcgtgctgc gtttgcagca gcgcgcggta cgtcccgcct
     3301 tgatggacgg ttccggcatt ctcaccgcgt ggctgctggc cctgtcgctg ccgccattcg
     3361 caccgtggtg gatcgcggtg ctgggcagcc ttttcgcggt gatcatcggc aagcagctat
     3421 tcggcgggct ggggcagaac gtattcaatc cggcgatggc cgcacgcgtg atgctgctga
     3481 tctcctttcc gctggagatg acctcgtggg tcgctccgca gggcgcaagt ggcccgggcc
     3541 tgctggaaag tctgcgcgtg acttttttca gtcagccgct ggacggtatc gccagcgctt
     3601 ctctgctcgg tcacgtgaag accgaattca cgcgtggcat cccgctggat caggcgttgt
     3661 ccggttacta cgcaccgctg gatgcgctgt ggggcagtcg tgcgggcagt ctgggcgaga
     3721 cggccgcact gttcctgctg gcgggcggac tgttcctgat cgccagacgc atcatcacct
     3781 ggcatgcacc ggtcgcgatg ctgctcggtg tggtcgtgcc cgcgctgttg ttcaacgcga
     3841 tcgacggcaa ccactatgcc gggccgctgg tgcacctgct gtcgggcggg ttgatgctgg
     3901 gcgcgttctt cattgtcacc gatccggtga cttcgccgaa cactgcgctc gggcagttcc
     3961 tcttcgggct gggttgcggc ctgctgacct ggatcatccg cacttggggc aactatccag
     4021 agggcgtggc gttcgcggtg atgctgatga acgcggccac gccgctgatc gaccagttga
     4081 tcaaaccgcg catctacggt cgcgaccgta agggtgcggc attgcctgtg aagaagggat
     4141 cgtgagatga agctcgatga attgcgcgga aaactgggtt atcaggggct gctgctcggc
     4201 ggggcggcgc tgctcaccag cgcggcgctg gcctttgcat cgcgcgccac agcgccggac
     4261 attcaggcgg ccgaggcggc cgatctcaag caatcgctca cgcaggtgtt gaccggagaa
     4321 tacgataacg acctgctcaa ggatacggtg ctcttgtccg gtgcggctgg agaggtgacg
     4381 gtgtatcgcg cccgccgtgc aggccgtgtc gaagcagtgg tgtatcgcgt ggtgggacag
     4441 ggctatgccg gttccatcca gtgtgtgatg ggggtgtcgc gcgagggaaa aattatgggc
     4501 gtgcgcgtca tcaaacatgc cgagacgccg ggcttgggcg acaagatcga acctgccaag
     4561 agcagctgga tacacgactt cgaaggcaag aaactgggcg aacccaatgc tgacaagtgg
     4621 gcggtgaaga aggacggcgg cgtgttcgac cagttcgccg gcgccactat cacgccgcgc
     4681 ggtgtggtca gggcggtgaa gggcggactg gcgttctttg ccgaacaacg cgagaagatg
     4741 ctggatgaat cttccggaaa aggagcgcag ccatgaacga gaaatacgca cgtatcaccc
     4801 gcgacggact gtgggacaac aacgtggtgt tcgcgcagat gctcgcgctg tgccccacgc
     4861 tggccgtgac cagcagcgcc accaacgggc tcggtatggg gttggcgacc acggcggtgc
     4921 tggtggtatc caacgtgatc atttcgatgg tgcgcgagct ggtcagcgcg gaagtgc
//
LOCUS       DASUIV010000106         8164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1048099, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000106 DASUIV010000000
VERSION     DASUIV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048099"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /gene="nuoE"
                     /locus_tag="VFW59_03900"
     CDS             complement(<1..368)
                     /gene="nuoE"
                     /locus_tag="VFW59_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX5363386.1"
                     /translation="MLSEQIQAKVDRELKKYPADQKQSAVMAALRFVQDEKGWISDDD
                     MAQVADYLGMPNMAVKEVATFYHMYNLKPMGKHTITICTNLSCTLCGALDTVDYIETK
                     LGIKLGEVTPDGKYGLREGE"
     gene            complement(368..1621)
                     /locus_tag="VFW59_03905"
     CDS             complement(368..1621)
                     /locus_tag="VFW59_03905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5363387.1"
                     /translation="MAEIKNYTMNFGPQHPAAHGVLRLVLELDGEVIERADPHIGLLH
                     RATEKLAENKTFLQSLPYMDRLDYVSMMSNEHAYVMAVEKLAGIEVPLRAQYIRVMFD
                     EITRILNHLLWLGAHGLDVGAMTMFLYCFREREDLMDAYEAVSGARLHAAYYRPGGVY
                     RDLPDTMPQYQASKIHNAAAVKRLNENRQGSLLDFLDDFTARFPKCVDEYETLLTDNR
                     IWKQRLVGIGVVTPERALALGLTGPMLRGSGVEWDLRKKQPYEVYDRLDFDIPVGVTG
                     DSYDRYLVRVEEMRQSNRIIKQCIDWLRVNPGPVMASNHKFSPPKREAMKQNMEELIH
                     HFKLFTEGMHVPAGEAYAAVEHPKGEFGIYMISDGANKPYRMKIRAPGFAHMSALDEM
                     CRGHMIADVVTIIGTQDIVFGEVDR"
     gene            complement(1633..2223)
                     /locus_tag="VFW59_03910"
     CDS             complement(1633..2223)
                     /locus_tag="VFW59_03910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5363388.1"
                     /translation="MAADLSTLRDNLAAMLGDKLVGADEKLGELTVVVKAAEMIDVLT
                     RLRDDAGFEQMIDLCGVDYSTYGDGAWEGKRFAIVYHLLSVAHNIRLRVRIFAEEDDF
                     PVLESVVNVWPGANWFEREAFDLFGIVFTGHPDLRRILTDYGFVGNPFRKDFPLSGHV
                     EMRYDPEQKRVVYQPVTVEPREVTPRIVREETFGCN"
     gene            complement(2231..2707)
                     /locus_tag="VFW59_03915"
     CDS             complement(2231..2707)
                     /locus_tag="VFW59_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5363389.1"
                     /translation="MGIEGVLEKGVVTTTLDTIINYTRTGSLWPMTFGLACCAVEMMQ
                     AGASRYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQSKIKRTNTIAR"
     gene            complement(2698..3054)
                     /locus_tag="VFW59_03920"
     CDS             complement(2698..3054)
                     /locus_tag="VFW59_03920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5363390.1"
                     /translation="MLENYFPVLLFVCVGLAFGVVPIVLGKLVSPNRPDSAKLSQYEC
                     GFEAFEDARMKFDVRYYLVAILFILFDLEIAFMFPWAVVLKEIGTFGFVSMMIFLAIL
                     VVGFIYEWMKGALEWE"
     gene            complement(3157..3243)
                     /locus_tag="VFW59_03925"
     tRNA            complement(3157..3243)
                     /locus_tag="VFW59_03925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3207..3209),aa:Leu,seq:gag)
     gene            complement(3261..3596)
                     /gene="secG"
                     /locus_tag="VFW59_03930"
     CDS             complement(3261..3596)
                     /gene="secG"
                     /locus_tag="VFW59_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX5363391.1"
                     /translation="METLVWVVHVVAAVVLIGLVLIQHGKGADMGAAFGTGSAGSLFG
                     SGGSANFLSRSTAVAASVFFVTSLSLTYLYSHPAQPQGVMDKVGATVAPAAPATDAVD
                     VSKSKEIPK"
     gene            complement(3644..4402)
                     /gene="tpiA"
                     /locus_tag="VFW59_03935"
     CDS             complement(3644..4402)
                     /gene="tpiA"
                     /locus_tag="VFW59_03935"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5363392.1"
                     /translation="MRRKLVAGNWKMHGGLAQNAELLDAVRAGMSALTGVDCAVCVPF
                     PYLGQAQQKLSGSNVKWGAQDVHQLDKGAYTGEVSASMLRDFGCAYVIVGHSERRAIY
                     GETSQLVAEKFVAAQQAGLLPILCVGETLEQRESGVTEAVVAEQLDAVIGLAGVQALR
                     NAVVAYEPVWAIGTGKTASSAQAQAVHEFIRKRVAAQDGQIAAGLCILYGGSVKANNA
                     IELFAMPDIDGGLIGGAALVAEEFLAICRAGVRT"
     gene            complement(4529..5275)
                     /locus_tag="VFW59_03940"
     CDS             complement(4529..5275)
                     /locus_tag="VFW59_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5363393.1"
                     /translation="MLPEEIELTRLESEQGELEEQVVQTELALETCKAETAQFQHRYY
                     RVVGRLYAQLDELDAQIAVLQVSLHPADATVQAYARAAERQARRSAEEAGLIESQPAP
                     PQELTAELKLVYRRAARLMHPDRAVTEAERQRRTDLMAQVNRAYENGDQRTIERMIDA
                     YGQDPEAIAGDDIGSRIVKAIRRIAQLRRRLGEAQHELAEQQQSELFQLRQSVEDGEA
                     LGGDPLGELAQQLVRELSERRARLEMLQHL"
     gene            complement(5285..6748)
                     /locus_tag="VFW59_03945"
     CDS             complement(5285..6748)
                     /locus_tag="VFW59_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5363394.1"
                     /translation="MTEYEKLPSWSVLQQTDLSVRQQDSLIARGLSAFRERKKSELAL
                     NSQDGRYRQARTVFDQQVCNIRKKGWTEEERAALFASFKSLGRMATEKYGKAYFPLAW
                     FYNSGWRIMPCGQDDAGDSAILNDPALSARYEQSAFEWCVSNRHVEDPELWFDLAACY
                     LRGIAVEPDIEQSMHWLKKSAEHGHELAQNQLGRMYESGRGSEQSDVRAAYWYRRAAE
                     QGNAWAQAELGAMHLKGKGVEQDYGLAVSRFSKAAERGNVAAQCFLAWLYRDGRGVEQ
                     SYENSAFWFRKAAEQGDELAQYFLGVLCEEHPEAGLGDEQAVFWYTKAAEQDHAGSQK
                     QLGKMYREGRGVAQDDAQAVYWFRKAALQGNDWGQYYLGVMYREGRGVARDDVQALYW
                     LYKAAEQGLEQAKEQVERIRGEARASSCDEVVVVAQQAELFAPPVIRTRRFPAAVRSR
                     PSAGKRMGRETQARRARVSKRVSPFRRGTRSGGRARR"
     gene            complement(6862..7242)
                     /gene="panD"
                     /locus_tag="VFW59_03950"
     CDS             complement(6862..7242)
                     /gene="panD"
                     /locus_tag="VFW59_03950"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX5363395.1"
                     /translation="MQRTMLQAKLHRVRVTQSELHYEGSCAIDDDLLDAADIKEYQQI
                     DIYNVTNGERFTTYAIRAQRGSRTISVNGAAAHKASPGDILIIATYAMYSELELQKFH
                     PQLVYVDEHNCIVAKRDRIPVQAA"
     gene            complement(7300..8133)
                     /gene="panC"
                     /locus_tag="VFW59_03955"
     CDS             complement(7300..8133)
                     /gene="panC"
                     /locus_tag="VFW59_03955"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5363396.1"
                     /translation="MQKISTISELRARLAGERAVAFVPTMGNLHEGHIDLVRIAREQG
                     DCVVVSIFVNPLQFGPSEDFDRYPRTLEDDCAKLRGLADVVFAPAVEEMYPAQQTVFV
                     EPPPIANELCGAARPGHFRGMATVVLKLLNIVQPQVAVFGKKDYQQLHIIRRMVAELN
                     LPVRIVGGETVRAADGLALSSRNQYLNKAERVEAVFLSQTLQQMRQALLSGERDIERL
                     QQQAVSALAARGWGVDYVAVCNQSDLLPANSSQREWVILAAARLGVPRLLDNVEVVLA
                     G"
BASE COUNT         1852 a         2680 c         2033 g         1599 t
ORIGIN      
        1 cactcgcctt cacgcaagcc atacttgccg tccggggtga cttcgcccag cttgatgccc
       61 aatttggtct cgatgtaatc caccgtatcc agtgcgccgc agagcgtgca ggacaggttg
      121 gtgcagatcg tgatggtgtg cttgcccatt ggcttgaggt tgtacatgtg gtagaaggtt
      181 gccacttctt tcacagccat attcggcatg cccagataat cggcgacctg cgccatatca
      241 tcgtcagaaa tccagccctt ctcgtcctgc acaaaacgca atgcagccat cacggcagat
      301 tgcttctgat cggccggata cttcttcagc tcccggtcaa ccttggcttg tatttgttcg
      361 gataacatca gcggtctacc tctccaaaaa cgatgtcctg cgtaccgata atggtcacca
      421 cgtcggcgat catgtggcct cggcacatct cgtccagcgc agacatatgg gcaaaacctg
      481 gggcgcggat cttcatgcga tagggcttgt tggcgccgtc ggaaatcatg tagataccaa
      541 actcgccctt ggggtgttcc accgccgcat aggcctcacc ggcaggcaca tgcataccct
      601 cggtgaacag cttgaagtgg tggatcaatt cttccatgtt ctgcttcatc gcttcgcgct
      661 tgggcggcga gaatttgtga ttgctcgcca tcaccgggcc gggattgaca cgcagccagt
      721 cgatgcattg cttgatgatg cgattggact gacgcatctc ctcgacgcgc accagataac
      781 ggtcatagga gtcgccggtg acgcccaccg gaatatcgaa gtccagacgg tcatagacct
      841 cataaggctg tttcttgcgc agatcccact cgacacctga accgcgcaac atcgggccgg
      901 tcagccccag cgccagggct cgctccggcg tgaccacacc gataccgacc agacgctgct
      961 tccagatacg gttgtcggtc agcagggttt cgtattcgtc cacgcacttg gggaagcgcg
     1021 cggtgaagtc atccaggaaa tcgagcaaag acccctgacg gttctcgttc aggcgcttga
     1081 ccgcagcggc gttgtgaatc ttggaagcct gatactgcgg catggtgtcc ggcaggtcgc
     1141 ggtagacgcc gcccggacgg tagtaggccg catgcaaacg cgcgcccgac actgcctcgt
     1201 aggcatccat cagatcttcg cgctcgcgga agcaatacag gaacatggtc atcgcaccga
     1261 cgtccagccc gtgcgcgccc agccataaca gatgattcag gatgcgggtg atttcgtcga
     1321 acatcacgcg gatgtactgc gcgcgcaacg gcacttcgat acccgccagc ttttcgaccg
     1381 ccatcacata ggcatgctcg ttgctcatca tcgagacgta gtcgagccgg tccatgtagg
     1441 gcagcgattg caaaaaggtc ttgttctcgg ccagcttctc ggtcgcgcgg tgcagcaggc
     1501 cgatgtgcgg atcggcacgc tcgatcactt cgccgtccag ctccagcacc aagcgcagca
     1561 caccgtgtgc agccgggtgc tgggggccaa agttcattgt gtaatttttg atttcagcca
     1621 tttagtgcat cctcagttac aaccgaaggt ttcttcgcgc acgatgcgcg gcgttacctc
     1681 gcgtggctcg accgtcaccg gctgatagac cacacgcttc tgctcggggt catagcgcat
     1741 ctccacatga ccggagagcg ggaaatcctt gcggaacgga ttgccgacaa aaccgtagtc
     1801 ggtcaggatg cggcgcagat cggggtggcc ggtgaatacg atgccgaaca gatcgaaggc
     1861 ctcgcgctcg aaccagttgg ctcccggcca gacattcacg accgattcga gcaccgggaa
     1921 atcgtcttcc tcggcgaaga tgcgcacacg caaacgaatg ttatgcgcca cggaaagcag
     1981 gtgatagacg atcgcaaagc gcttgccctc ccacgctccg tcaccatagg tggagtaatc
     2041 gacaccgcac agatcgatca tctgctcgaa tccggcatcg tcgcgcaagc gcgtcagaac
     2101 atcgatcatc tcggcagcct tgaccaccac ggtcaactcg cccaatttct catccgcgcc
     2161 aaccagcttg tcgccaagca tagcggccag attgtcacgc aacgtactca gatcagcagc
     2221 cataattcac ctagcgcgca atggtattgg ttcgtttgat cttgctctgc agctgaatga
     2281 tgccgtagag cagcgcctca gcagtcggag gacaacccgg cacgtacaca tccaccggca
     2341 cgatgcggtc acaaccgcgc accaccgagt aggaatagtg atagtaacca ccaccgttgg
     2401 cacatgaacc catggagatc acccagcgcg gctcggccat ctggtcatac accttgcgca
     2461 aggctggcgc catcttgttg cacagcgtac ccgccacgat catcacgtcc gactgacgcg
     2521 gactggggcg gaacaccaca ccaaaacgat ccaggtcgta gcgcgaagcg cccgcctgca
     2581 tcatctcgac cgcacagcag gccaagccga aagtcattgg ccacaacgaa cccgtacggg
     2641 tgtagttgat gatggtatcg agagtcgtcg tgacgacgcc cttctccaga acaccttcta
     2701 ttcccattcc aaggctcctt tcatccattc gtagataaag ccaaccacga ggatggccaa
     2761 gaaaatcatc atggagacaa aaccgaaggt gccgatctct ttaagcacga cagcccaggg
     2821 gaacatgaag gcaatctcaa gatcgaacag aatgaacaga atagccacga ggtagtagcg
     2881 cacatcgaac ttcatgcgcg catcttcgaa cgcttcgaag ccacactcgt attgagaaag
     2941 ttttgcgcta tccggacgat tgggagatac caacttgccg agaacgatcg gaaccacgcc
     3001 aaaagcaagc ccgacacaga cgaacaacag caccggaaaa tagttttcca acattgagct
     3061 tccccctcta gttgcggtga cctgtgaccc tcagcgatac agggatcact ttttagttta
     3121 tgtcgcctgc atacttgtgc ggcgacttct tatatttggt gcggatgggg agactcgaac
     3181 tcccacgccc tttcgggcac aagcacctca agcttgcgtg tctaccaatt ccaccacaac
     3241 cgcgatgcta caacaaaact ttatttcgga atttccttgg acttcgatac atcaacagca
     3301 tcggttgcgg gtgccgccgg agcgactgtc gcacccacct tgtccatcac tccctgcggc
     3361 tgcgcgggat gcgaatacaa ataggtcaac gacaaactgg taacaaaaaa taccgacgcg
     3421 gcgactgccg tgctgcgact gagaaaattg gccgagccac ccgagccgaa cagactacca
     3481 gccgaccccg taccgaaggc agcccccata tcggcgccct tgccatgctg aatcagcacc
     3541 aagccgatca gaaccacagc agctaccaca tgcaccaccc aaaccagagt ttccactcaa
     3601 accaccctaa acaaaaaatc aaacctagcc gggacgcaaa aaatcacgtc cggacacctg
     3661 cgcgacaaat cgccaaaaat tcctctgcca ccaaggcagc gccgccgatc aagccaccat
     3721 cgatgtccgg cattgcaaac agctcaatcg cattattggc cttaacgcta ccgccgtaga
     3781 gtatacacaa cccggcagca atctgaccat cctgagccgc tactcgctta cgaataaact
     3841 cgtgaaccgc ctgcgcttgc gccgaactcg ccgtttttcc ggtaccgatc gcccacaccg
     3901 gctcataggc cacaaccgca ttgcgcagcg cttgaactcc agccagaccg atcaccgcat
     3961 ccagttgctc ggccactacc gcctcggtga caccgctttc gcgctgctcc agcgtctcgc
     4021 cgacacagag gatgggcaac aaaccggcct gctgagcagc gacgaatttc tccgccacca
     4081 gctgactcgt ctcgccataa atggcgcgac gctcggagtg cccgacgatc acataagcac
     4141 agccgaaatc ccgcaacatc gacgccgaaa cctcaccggt atatgcacct ttgtccagct
     4201 gatgcacatc ctgagcaccc cactttacat tgctgccgga caacttctgc tgcgcctgcc
     4261 ccaagtaagg gaacggcaca caaaccgcgc aatctacgcc agtcaaagcc gacatcccag
     4321 ctcgcaccgc gtccagcaac tcggcgttct gcgccaagcc accatgcatt ttccagtttc
     4381 cagcaaccag tttgcgtcgc atcatattga tcaatccgct agacatcgaa gcgcgcaatc
     4441 gtacccgcga acccgaggga ggtcaataat acccagtgcc gtatcgcgcg acatggcgtt
     4501 aactctttca gaaaagacga acaaatattc acagatgctg caacatttcc agccgcgccc
     4561 tgcgctcgga cagctctcgc accagttgct gcgccaattc gcccagagga tcccctccca
     4621 atgcttcccc atcttcgaca ctctgcctga gctgaaatag ctcgctttgc tgctgctcag
     4681 ccagctcatg ctgcgcctcg cccaagcgcc gacgcaattg tgcgatgcgg cgaatcgcct
     4741 tgacgatgcg ggagccgata tcgtcacccg caatcgcctc aggatcctgt ccatacgcat
     4801 caatcattct ctcaatggtc cgctggtcgc cattctcgta ggcacgattc acttgcgcca
     4861 tcaaatcagt gcggcgctgc cgctccgcct cggtaacggc gcgatccgga tgcatcaatc
     4921 gggcagcgcg ccgataaacc aacttgagct ctgcagtcaa ttcttgcggt ggcgcaggct
     4981 gcgactcgat cagccccgcc tcctctgccg aacgcctggc ctgtcgctca gcggcacggg
     5041 cataggcctg cacggtcgca tcggcaggat gcagactcac ctgcaacacc gcgatctgcg
     5101 catccagctc atccagctgg gcatacaaac gccccaccac ccggtagtag cgatgttgaa
     5161 attgagcggt ctcggccttg caggtttcca gcgccagctc ggtctgcacg acctgctctt
     5221 ccagctcgcc ttgctcactt tccaaacggg tgagttcgat ttcttcagga agcatgaccg
     5281 cccctcatcg acgcgctcta ccgccagaac gagtccctct cctgaacggg gaaacacgct
     5341 tgctgacacg tgcacgccgg gcttgcgttt cccgccccat ccgcttgccg gcacttggcc
     5401 gactgcgcac agcagccggg aaacgtctgg ttctgataac aggaggcgcg aacaactcag
     5461 cctgctgagc gaccaccacg acctcgtcgc atgacgacgc ccgcgcctcg ccacgaatcc
     5521 gctccacctg ctctttggcc tgctccaacc cctgctccgc tgccttgtac agccagtaca
     5581 gcgcctgaac atcgtcgcgc gccaccccgc gcccttcccg atacatcacg cccagatagt
     5641 actgccccca atcgttaccc tgcagcgcag ccttacggaa ccagtagact gcttgcgcat
     5701 catcctgtgc caccccgcgc ccctctcggt acatcttacc gagctgcttt tgactgcccg
     5761 catgatcctg ctcggcagcc ttggtatacc agaacacagc ctgctcatcc cccaacccgg
     5821 cctccggatg ctcttcacac agcacaccaa gaaaatactg cgccagctca tccccctgct
     5881 cggcagcctt acggaaccag aacgcagaat tttcataact ctgctccacg ccgcgaccat
     5941 cccggtacaa ccaggcgaga aaacactgcg ccgcgacatt gccgcgctcg gccgccttgc
     6001 tgaagcgact cacggccaga ccataatcct gctcgacgcc cttgcctttc agatgcatgg
     6061 cacccaattc ggcctgtgcc caggcattac cctgctcggc ggcgcggcga taccagtacg
     6121 cagcccgcac atcgctctgc tcgctgccgc gcccgctctc atacatccgg cccagttggt
     6181 tctgcgccaa ttcatgacca tgctcagccg attttttcag ccagtgcata gactgctcaa
     6241 tatccggttc gaccgcgata ccacgcaagt aacaggcagc cagatcaaac cataactcgg
     6301 gatcctcaac atgccggttg ctgacacacc actcgaaggc agactgctca tagcgcgccg
     6361 acagggcggg atcattcaga atggcagaat cgccggcgtc atcctgaccg cagggcatga
     6421 tgcgccagcc cgagttatag aaccacgcca atgggaaata ggccttaccg tacttctcgg
     6481 tcgccatccg gcccaacgac ttgaatgagg cgaacaaggc tgcccgctct tcctcggtcc
     6541 agcctttttt gcgaatgtta cagacctgtt gatcaaacac cgtgcgcgcc tgacggtaac
     6601 ggccatcctg actgttaagc gccaattcac tcttcttgcg ctctctgaaa gccgaaaggc
     6661 cacgagcgat caggctatcc tgctggcgca ccgacagatc agtctgctga agaaccgacc
     6721 aggaaggaag tttctcgtac tcagtcattc aggcaccaca tacgaaaagg cgatcgatca
     6781 agccgcctcc tgtcgcacgg aacgatccac gcattcccgc aggaggctca aacatctcac
     6841 gccaagccgc tccatgcagc cctaggcagc ctgtaccggt atccggtcgc gcttggcgac
     6901 gatgcagttg tgctcgtcga catagacgag ttgcggatga aatttctgca actccagctc
     6961 agaatacatc gcataggtcg cgatgatcag gatatcgcca gggctggctt tatgggccgc
     7021 tgcgccgttt acggaaatcg tccttgagcc acgctgcgca cgaatggcat aggtcgtgaa
     7081 acgttcacca ttggtgacat tatagatatc gatctgctga tattccttga tatcagcggc
     7141 atccagcaaa tcgtcgtcga tcgcgcagga accctcatag tgcaactcgg actgcgtgac
     7201 gcgcacccgg tgcaacttgg cctgaagcat ggttctctgc atgaaaattc tccaatttcg
     7261 acgaacgcgg attatatcgc actcgcacca ctcccggcat caaccggcaa gaacgacctc
     7321 gacattatcc agaagacggg gaacccccag cctggctgcc gccaaaatca cccactcgcg
     7381 ctgcgaagaa ttggcgggta acaaatcgga ctggttacac actgccacat aatccacccc
     7441 ccatcccctt gcagccaagg ctgagaccgc ctgttgctgc aaacgttcaa tatcgcgctc
     7501 gcccgacaaa agcgcctgcc gcatctgttg cagcgtctgg gacagaaaca ccgcttcgac
     7561 gcgctctgcc ttgttcaaat actgattgcg cgaactcagc gcgagaccgt ccgcagcgcg
     7621 caccgtctca ccaccgacga tacgcaccgg cagattcaat tccgcaacca tccggcgaat
     7681 gatgtgcagc tgctgataat ccttcttgcc gaacaccgcg acctgcggct gcacaatatt
     7741 cagcagcttc aacaccaccg tcgccatacc tcgaaaatgg ccgggccgcg ccgcgccaca
     7801 gagctcattg gctatcggcg gcggctcgac gaataccgtc tgttgcgcgg gatacatctc
     7861 ttcgacggca ggtgcgaaca ccacatcggc caatccgcgc aacttggcgc aatcgtcctc
     7921 cagagtacgc gggtagcgat caaagtcttc actgggaccg aactgcagcg gattaacaaa
     7981 aatgctcact acgacacagt cgccctgttc gcgcgcgatg cgcaccagat cgatatgccc
     8041 ctcgtgcaaa ttgcccatgg tgggcacgaa ggcgacggcg cgctcgcctg caaggcgtgc
     8101 gcgcagctct gaaatggttg agattttttg catcgtttgt tctctacttt ggattggttg
     8161 gtac
//
LOCUS       DASUIV010000107         9904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_491265, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000107 DASUIV010000000
VERSION     DASUIV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9904
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491265"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFW59_03960"
     CDS             <1..330
                     /locus_tag="VFW59_03960"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5363397.1"
                     /translation="LPELAQDMASFLYQLSQLPLDAGVIPIYISNGKKLERYELAVGA
                     EEYIQTDFGRLRALPLHKIHGSNEEGLDIWLGLEYRLLPIKIRQTDREGHTAGEMVVS
                     DIRVADE"
     gene            complement(337..834)
                     /locus_tag="VFW59_03965"
     CDS             complement(337..834)
                     /locus_tag="VFW59_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363398.1"
                     /translation="MCIAVIWVELVVPGLQTCQRVKREGEMVFEWTDSLSVGNAMMDA
                     DHQNLIGMIRAVEHALRSGDCVVLSQSFQLLLYGVGIHFANEERLALALELPFDQHKK
                     LNRAQQYELEHMRTELEAKSGIWSGGAVEHFSCSLRNWLSEHIGRDGERLRPVLAAHP
                     YHFKP"
     gene            974..2227
                     /locus_tag="VFW59_03970"
     CDS             974..2227
                     /locus_tag="VFW59_03970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX5363399.1"
                     /translation="MLITEYRIDLVILALRTILPLEHPADTTLRYFFQDNRIGSNERA
                     LVAETVFGVLRHRLLLEHACGDTPTPRRMGLAWLLKFGGYNLREIEPVLKRDEKEWLA
                     TVKAVKPETLPLSIQAELPDWLLEKMRVSYSDEEILAIGLAMQQGAPLDIRVNTLLAK
                     REEVLQQLHDKHIEATVTPYSPIGIRLKEKIPLNKDALFTEGKVEVQDEGSQLLGFLL
                     APKRNDMVVDFCAGAGGKTLMLSAQMNSQGRLYAMDVSEKRLTNLKPRLKRSGASNIQ
                     PMLIAHENDLKVKRLAGKIDRVLVDAPCSGLGTLRRNPDLKFRQSPQSLAELTKKQAA
                     ILASASRLLKKGGRLVYATCSILPEENQDIVRNFLATHPDFVLKPAGEVLQQQKIALE
                     MGDYLELRPQLHGTDGFFAAVLERV"
     gene            complement(2261..3886)
                     /locus_tag="VFW59_03975"
     CDS             complement(2261..3886)
                     /locus_tag="VFW59_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5363400.1"
                     /translation="MYSSDNLLDRLKELNEIGIALSKQRNINLLLETILDAAKRITHA
                     DAGTLYSVEPEQRVLSFEILRNDSLGSAIGGSSGVPITFPTIRLYDDAGNPNHNNVAS
                     HSALSGETINIPDAYIAEGYDFSGTKKFDAQSGYRSKSFLTVPMRNHEDEVIGVLQLI
                     NALDRETGAIIPFSRDDQQLLESLASQAAIALTNHRLIAQMEALFESFVQLINTAIDD
                     KSPYTGGHCERVPVLTMMLAEAVNRTREGPLKDFVMTERDRYELKIAGLLHDCGKITT
                     PVHVVDKATKLQTLFDRIHLVDTRFEVLKRDAEIVMLREMLAAAGDHARVEAARSTHV
                     ERMAELDEGREFLRRCNVGGEAMSDADIQRVQRIASDRWCDSTGQEANFLSDNELENL
                     SIRFGTLTADEREIIKRHIEITIKMLESLPWPKHLKKVPEYAGGHHERMDGKGYPRGL
                     MREQMSVQARVMGIADIFEALTAKDRPYKQGKTLTESLTILGNLKLNGHIDPDLFDVF
                     IREKVYLDYARQFLPPEQIDEVDLSKIPGVNLN"
     gene            complement(3911..6307)
                     /locus_tag="VFW59_03980"
     CDS             complement(3911..6307)
                     /locus_tag="VFW59_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5363401.1"
                     /translation="MGKEVSGSNAADDVAVGVRLEFTKNLNHVINKIHATSNIDEIML
                     DVSKDICTMFNADRLTIYVVGDDNVSLISKVKTGLNSFKDLKLPIAEQSIAGFAAMHK
                     KLLNIRDVYDEAELATYSSHLRFLQDVDKRTGYRTKQLLVAPILEANTNELVGIIQVI
                     NNKAGVPFSMMVEEGVQELAQTMAVALRQRQQSSTVKTKYDYLVADAVLSAAEFDLAT
                     RTARRKGIDIEEVLIDEFQVSIQALGQALSRFFGVPYEPYKSDRIKPSDLLKNLKREY
                     IESSHWLPIEDNQEGLLILTTDPERIQASRVVNNIFPKHRLVYKVCSQREFKQTLDLF
                     YGGGALDGTGGFSADESSMDDLLSSLGGDDEESGTVTQEDVNAAADNELVKLVNKIIV
                     DAYRMGASDIHIEPGPGKAKTRIRVRKDGSLLNYIEVPSTYRNALVTRLKIMCDLDIS
                     EKRKPQDGKIKFKKYGPLDIELRVATIPSQGGVEDVVMRILASGEPLPLEKMGFSARN
                     NEQIQATISKPYGLFFVCGPTGSGKTTTLHSILKYINTPDTKIWTAEDPVEITQKGLR
                     QVQINKKAGLDFATIMRAFLRADPDVIMVGEMRDKETVSIGIEASLTGHLVFATLHTN
                     SAPESIIRLLDMGMDPFNFADALLGILAQRLAKRLCGDCKKPHTASAEEMQALLTEYS
                     DELKNIPSWKADPAAASKALYAEWVRLYADEKGQFTIYEPVGCEKCSGTGYRGRVGLH
                     ELLIGTDPVKKAIQEHARVAELLAIALEEGMHTLKQDGMEKVLQGITDMHQIRAVCIK
                     "
     gene            complement(6393..7157)
                     /locus_tag="VFW59_03985"
     CDS             complement(6393..7157)
                     /locus_tag="VFW59_03985"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEX5363402.1"
                     /translation="MKLRVLGCSGGIGGNLRTTSFLLDHDVLIDAGTGVGELSLSELK
                     VVDHIFITHSHLDHIACLPLLIDSVGFMRDRPLTLYATAETLDILRQHIFNWKVWPDF
                     SQIPNAERPYLRFQAIALNETIALDGRKITAIPALHTVPAVGFRLDSGRASLVFSGDT
                     TNNDALWEVVNRIENLRYLIIETAFSDSERELAILSKHLCPSMLAEELLKFRGEAEIY
                     ITHLKPGEVELTMRQIEADVQGRQLQMLLNNQEFEF"
     gene            complement(7193..9415)
                     /locus_tag="VFW59_03990"
     CDS             complement(7193..9415)
                     /locus_tag="VFW59_03990"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5363403.1"
                     /translation="MKKNAVLIGLGLLIVLVFVGHAAHFYRIGIVDRLSAIVYDYRLR
                     LTMPRTVDERIVIIDIDEKSLKEEGRWPWGRNRLGELMDKLFDKYGVALVGFDVVFAE
                     KDESSGLGVLQRLGQNQLRDDASFQSALAQIRPQLEYDRLFAEKIRNRPVVLSYYLTE
                     QQATHVSGMLPEPAFPAGTFAGRPIEFTGWNGYGANLPELQQAAAGAGHFNPLVDSDG
                     VVRRIPMLAEYNGAYYESLSLAMVRALLGQPPLRPGFAEGRTDGYAGLEWLELDAASG
                     TLKIPVDANVAALVPYRGEQGSFRYLSIADVLHDRIDPAQLKDKIVLVGTSALGLMDM
                     RATSVAEVFPGVEIHANLIAGILDQNLKVRPPWMLGAEVLWLLVVGITLSFLLPMLSP
                     IKVMLVAMSMLAMTAGLSLAAWQYGDVVMPMANSLLMISLILALDMSYGYFFESRTRR
                     QITGLFGQYVPGELVEEMAKHPESISMAGKSSEMTILFADVRGFTSISEGLDPRELTL
                     LMNEFLTPLSRVIYKHRGTIDKYMGDCIMAFWGAPLPDAEHSRHAILAGLEMQTTLKA
                     LQPSFKERGWPEIRIGVGINTGRVSVGNMGSEVRVAYTAMGDAVNLASRLEGLTRQYG
                     VEIIVGENTRNAVADFVYRELDHVRVKGKDKPVAIYEPLGLSGEVSPEALEELQLFHE
                     ARRLYRKRDWDMAELQLLSLQRMSPDTALYGVYVERVAYFRKNPPGDDWDGVFVFKTK
                     "
     gene            complement(9487..>9904)
                     /locus_tag="VFW59_03995"
     CDS             complement(9487..>9904)
                     /locus_tag="VFW59_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5363404.1"
                     /translation="ASAEPAISQTQVNPRIAAVEEVEKTGRFGSLQPAQPAAVPTAAA
                     PKAAAAPQAAAVQILTGPNAGKELELIKNLTTLGKPGVQVAVLTRRPHGFFITHVEGA
                     DHPLVNGQTLGDQPHQLQDHDMIELAGIKMEFYFKE"
BASE COUNT         2183 a         3016 c         2584 g         2121 t
ORIGIN      
        1 ctgccggagc tcgcccagga catggccagc ttcctgtacc aattatcgca actgccgctg
       61 gacgcaggcg tcataccgat ctacatcagc aacggcaaga agctggaacg ctacgagctc
      121 gcggtcggcg cagaagagta catccagacc gacttcggca ggctacgcgc actgcctctg
      181 cacaaaatcc atgggtcaaa cgaagaaggg ctggatatct ggttgggact ggaataccgg
      241 ctgctgccca tcaaaattcg tcaaacagac cgcgaaggac atacggctgg cgagatggtg
      301 gtctcggata tccgcgtagc ggacgagtag cccggatcag ggcttgaaat gataggggtg
      361 cgccgccagc acaggcctca atctctcgcc atcccgcccg atatgctcgc tcaaccagtt
      421 cctcagcgaa cacgagaaat gctcgaccgc cccgcctgac cagatgccac tcttggcctc
      481 cagttcggtc cgcatatgct caagctcgta ctgctgcgca cgattcaatt ttttgtgttg
      541 atcgaaaggc agttcgagtg ctaacgcgag cctttcctcg ttggcaaagt gaatacccac
      601 gccgtagagc aggagctgaa atgactgcga cagaacgacg caatccccgg atctgagcgc
      661 atgctccacc gcgcgtatca tgccgatcag attctgatgg tcggcatcca tcatggcgtt
      721 gcccacactc aagctatcag tccactcgaa caccatctca ccctcccgtt taacgcgctg
      781 gcaggtctgc aagcccggca cgaccaactc tacccagatg acagcaatgc acaatatgcc
      841 ggaaagccta tatatttagg cctaagcatc taaaggctgt tccaattggc tttgcgcgcc
      901 atccagagga tggtgacgat attgcatgca caaccctgtt aaactgcgcg cctgaaacag
      961 gaaatccatt accatgctta ttaccgaata ccgtatcgac ctcgtcatcc tggcgctgcg
     1021 caccatcctg ccgctggaac atcccgccga caccacgttg cgctatttct ttcaggacaa
     1081 ccgcatcggc tccaacgaac gcgcgctggt tgccgaaacc gtattcggcg tgctgcgcca
     1141 ccgcctgctg ctggaacatg cctgcggtga cacccctacc ccgcgccgca tgggtctggc
     1201 ctggctgctc aaattcggcg gctacaatct gcgcgagatc gaacccgtac tgaagcgcga
     1261 tgaaaaggaa tggctcgcca cggtcaaggc cgtgaagcct gagacgctgc ctctgtcgat
     1321 acaggccgag ctgccggact ggctgctgga aaagatgcgc gtgtcctatt ccgacgaaga
     1381 gattctcgcc attggtctgg ccatgcagca aggcgcgccg ctggacatcc gcgtcaacac
     1441 cctgctcgcc aaacgcgaag aggtgttgca acaactgcac gataaacaca tcgaagcgac
     1501 cgtcaccccg tactcgccga tcggcatccg cctcaaggaa aagatcccgc tgaacaagga
     1561 cgcgttgttc accgaaggca aggtcgaagt acaggatgaa ggcagccagt tgctcggctt
     1621 tttgctcgca cccaaacgca acgacatggt ggtggatttc tgcgcggggg caggcggaaa
     1681 gacgctgatg ctgtcggcgc agatgaattc gcagggccgc ctgtatgcga tggacgtatc
     1741 cgagaagcgc ttgaccaacc tcaagccgcg cctgaagcgc tccggcgcca gcaacatcca
     1801 gccgatgctg atcgcacatg agaacgacct taaggtgaaa cgtctggcag gcaagatcga
     1861 ccgcgtactg gtggatgccc cctgcagcgg cttgggtaca ctacgccgca acccagacct
     1921 gaagttccgt cagtcgcccc agagtcttgc agaactcacg aaaaagcagg ctgccatcct
     1981 cgcctccgcc agccgtttgc tgaagaaggg cggacgcctg gtctatgcca cttgcagcat
     2041 cctgccggaa gagaaccagg atatcgtgcg gaacttcctc gccactcatc ccgatttcgt
     2101 gttgaaacct gcgggcgaag tgttgcaaca acagaagatc gcactggaga tgggtgacta
     2161 tctggaactg cgcccacagt tgcatggcac agatggattc ttcgcggcgg tgctggaacg
     2221 ggtctgattc tcatcgatcc tgaacaaccg ctttacgggt tcaattcaga ttaacgcccg
     2281 ggattttgct caaatccacc tcgtcgatct gctcgggcgg caggaattga cgcgcatagt
     2341 ccagatagac cttctcgcgg atgaagacat cgaacagatc cggatcgatg tgaccgttca
     2401 attttaaatt gccaagtatg gtcagtgact ctgtcagggt cttcccctgt ttgtagggtc
     2461 tatccttggc cgtcaatgcc tcgaagatat cggcgatccc catcacgcgg gcctgcaccg
     2521 acatctgttc tcgcatcaag ccgcgcggat agcctttgcc atccatgcgc tcgtgatgcc
     2581 cgcccgcata ttccggaacc ttcttgagat gtttgggcca aggcagcgac tccagcatct
     2641 tgatcgtgat ctcgatgtga cgcttgatga tctcgcgctc atccgccgtc aatgtcccga
     2701 aacgaatgga aagattctcc aattcgttgt cactgagaaa attggcctcc tgtccggtgc
     2761 tatcacacca gcggtcagac gcgatacgct ggacgcgttg tatatccgca tcggacatcg
     2821 cctcgccgcc gacattgcaa cgacgcaaga actcgcggcc ctcatccagc tccgccatgc
     2881 gttcaacatg ggtcgaccgc gccgcctcga cacgggcatg atcgcctgct gcggcaagca
     2941 tctcgcgcaa catcacaatc tcggcatcgc gcttgagcac ctcgaaacga gtgtcgacca
     3001 ggtgaatgcg gtcgaatagc gtttgcagct tggttgcctt gtcgaccaca tggactggcg
     3061 tggtgatctt gccgcaatcg tgcagcaggc ccgcaatctt gagttcatag cgatcccgtt
     3121 cggtcatcac gaaatccttc agcggcccct ctcgggtgcg gttgaccgcc tcggccagca
     3181 tcatggtcag caccggcaca cgctcgcagt gtccaccggt atagggcgat ttatcgtcga
     3241 tcgcggtgtt gatcagctga acaaaggact cgaacaacgc ttccatttgc gcgatgagac
     3301 gatgattcgt caacgcgatg gcggcctgtg aagcgagcga ctccagcagc tgctgatcgt
     3361 cccgcgagaa tgggatgatc gctccggtct cccgatccag tgcgttaatg agttgcaaca
     3421 ccccgatgac ttcgtcttca tggttgcgca tcggcacggt gaggaacgat ttggaacgat
     3481 agccggactg ggcgtcgaat ttcttggtgc cggaaaaatc gtaaccttcc gcgatgtagg
     3541 catcgggaat gttgatcgtc tcgccgctca atgccgaatg gctcgcgacg ttgttgtggt
     3601 tgggattgcc ggcgtcatcg tacaggcgga tcgtcgggaa agtgatcggc accccgctgc
     3661 tgccgccgat cgcactgccc agggagtcat tgcgcaatat ctcgaaactc agcacccgct
     3721 gctccggctc gaccgaataa agcgtaccgg catcggcgtg ggtgatgcgc ttcgccgcat
     3781 cgaggatcgt ctccaacaag agattgatgt tccgctgctt ggacagagcg ataccgatct
     3841 cattcagttc cttgaggcga tccagcaaat tatccgaaga gtacatatgg ctgtcgggca
     3901 atggtctctg ttatttaata cagacggcgc ggatctggtg catgtcggtg atgccttgca
     3961 gcaccttctc catgccatcc tgcttcagcg tgtgcatccc ttcttccagc gcgatcgcca
     4021 gcaattccgc gacccgcgca tgctcctgaa tcgccttctt gaccgggtcg gttccgatca
     4081 gcaactcgtg caaaccaaca cggccgcggt agcccgtacc ggaacatttc tcacagccga
     4141 ccggttcata aatggtgaat tgccccttct catcggcgta tagtctgacc cactcggcat
     4201 agagcgcctt gctcgccgct gccggatcgg ccttccagga cggaatgttc ttcaactcat
     4261 cgctatactc ggtgagcaaa gcctgcattt cctcggcgct cgcagtgtgc ggttttttgc
     4321 agtcaccgca caggcgtttg gccagacgtt gcgccaggat gcccagcagc gcatcggcga
     4381 agttgaacgg atccatcccc atatcgagca gacggatgat ggattccggg gcgctgttgg
     4441 tgtgcagggt ggcgaacacc aggtgaccgg tgagcgaagc ctcgataccg atcgacacgg
     4501 tctccttgtc gcgcatctcg ccgaccatga tcacgtcggg atcggcacgc aggaaagctc
     4561 gcatgatggt ggcgaagtcc aatccggcct tcttgttgat ctgcacctga cgcagcccct
     4621 tctgggtgat ctccaccggg tcttccgccg tccagatctt ggtgtcgggg gtgttgatgt
     4681 atttaaggat ggagtgcagc gtggtggtct taccggaacc ggtcggaccg cacacgaaga
     4741 acagaccgta tggcttgctg atcgttgcct gtatctgttc gttattgcgc gccgagaagc
     4801 ccatcttttc cagcggcaac ggctcgccgg aagccaggat acgcatcacc acgtcttcca
     4861 cgccgccctg agaaggaatg gtcgccacgc gcagctcgat atccagcggc ccgtatttct
     4921 tgaacttgat cttgccgtcc tgcggcttgc gcttctccga gatatccaga tcgcacatga
     4981 tcttgaggcg cgtcaccagc gcattgcggt aggtggaggg cacctcgata tagttcagca
     5041 gcgaaccatc cttgcgcacg cggatccgcg tcttggcttt accgggcccc ggttcgatat
     5101 ggatatcgga tgcgcccatg cgataggcat cgacgatgat tttattgacc agcttgacca
     5161 gttcgttatc ggccgcagca ttgacatctt cctgagtaac ggtgcccgac tcttcatcgt
     5221 ctccgcccag actggacaac agatcatcca tcgatgactc gtctgcggaa aaaccgccgg
     5281 taccgtcaag cgccccgccg ccatagaaca gatccagcgt ctgcttgaac tcgcgttgag
     5341 agcagacctt gtagaccaga cggtgcttgg ggaagatgtt gttgaccacc cgcgaagcct
     5401 gaatacgctc cgggtcggtg gtaaggatca gcaacccctc ctgattgtcc tcaatcggca
     5461 gccagtgact gctctcgata tactcgcgct tcaggttctt gagcagatcg gacggtttga
     5521 tacgatccga tttgtagggt tcgtagggaa cgccgaaaaa gcgcgacaac gcctggccta
     5581 gcgcctggat gctgacctga aattcgtcga tcagcacctc ctcgatatcg atccccttgc
     5641 gtcttgccgt acgggtcgcc agatcgaatt ccgcagcaga caacacggca tcggcaacca
     5701 gatagtcgta cttggtcttg accgtgctgc tttgctggcg ctggcgcagc gccaccgcca
     5761 tggtctgggc cagctcctgc accccttcct cgaccatcat ggagaacggc acgccggcct
     5821 tgttgttaat gacctggatg atgcccacga gttcgttggt attcgcttcc agaatcggag
     5881 caaccagcag ttgcttggtg cgataaccgg tacgcttgtc tacgtcctgc aggaagcgca
     5941 aatgcgagct ataggtcgcc agctcggcct cgtcatagac gtcccgaata ttgagcagct
     6001 tcttgtgcat ggccgcaaag cccgcgatgc tctgttcggc gatgggcagc ttgaggtcct
     6061 taaatgaatt gagcccggtc ttcaccttgg agatcagcga gacgttgtca tcccccacca
     6121 catagatagt caaacgatcg gcattgaaca tcgtgcagat gtccttgctc acgtccagca
     6181 tgatctcatc gatattgctg gtggcatgta tcttgttgat gacgtgattc aggttcttgg
     6241 tgaattcaag ccgaactccc accgccacgt catctgccgc attgctgcct gacacttctt
     6301 tgcccattcc tgctcccctg ttcttcactg cgactgcatt aattccgatg tcaccacaac
     6361 actttcgaca tcaagtgtca gctgtcacca agctaaaatt caaattcctg attgttcagc
     6421 aacatctgga gttggcggcc ttgcacatca gcctcgatct gccgcatggt caactcaact
     6481 tcacctggct tcaggtgcgt gatataaatt tctgcctcgc cacggaattt caacaactcc
     6541 tctgccaaca tgcttggaca cagatgtttg gacaagatgg caagttctct ctcgctgtcc
     6601 gaaaaggccg tttcaatgat cagataacgc agattctcta tcctgttcac cacttcccac
     6661 aacgcatcgt tgttggtggt atccccgctg aagaccaaac tggcccggcc gctatcaagc
     6721 cgaaaaccaa cagcaggaac ggtatgcaag gcaggaatcg cggtgatctt gcgcccatcc
     6781 aacgcaatcg tctcgttcag cgcgattgcc tgaaagcgca gataggggcg ctctgcattc
     6841 ggtatttgac tgaaatccgg ccacactttc caattgaata tatgttgccg cagaatgtcc
     6901 aacgtttctg ccgttgcata aagcgtcaac ggacgatcac gcatgaagcc gacactgtcg
     6961 atcagcaacg gcagacacgc gatatgatcc agatgagagt gtgtgatgaa aatatggtcg
     7021 accactttca gctcggacaa gctgagctcg cctaccccgg tgcccgcatc gatcagcaca
     7081 tcgtggtcga gcaaaaaaga ggtggtgcgc aagttcccac ctattccgcc actacaccct
     7141 aaaaccctca gcttcattat ctgccagcct ttgctgttgt catcaacgcg aactactttg
     7201 ttttaaacac gaacacgccg tcccagtcat caccaggcgg gttcttgcgg aaataggcga
     7261 cgcgctcgac ataaactccg tacaacgccg tatccgggga catccgctgc aaactcagca
     7321 attgcaactc cgccatatcc cagtcccgct tgcgatataa acgccgcgct tcatggaata
     7381 gctggagttc ctccaacgct tcagggctga cctcaccgct caatccaagc ggctcataaa
     7441 ttgcaacggg cttatcttta ccttttaccc gcacatggtc aagctcgcga taaacaaagt
     7501 cagcaacggc atttcgggta ttttcaccaa cgatgatttc cacgccatat tgtctggtaa
     7561 gaccttcgag acgagaggcg agattgaccg catcgcccat cgcggtataa gccacacgca
     7621 cctcggaacc catgttgccg acgctgaccc ggccggtatt gatgccgaca ccgatacgaa
     7681 tctccggcca accgcgctcc ttgaatgacg gctgtagcgc tttcagggtc gtttgcatct
     7741 caaggccggc cagtatggca tggcgagagt gttcggcatc aggcaacgga gccccccaga
     7801 acgccatgat gcaatcgccc atgtatttgt ctatggtgcc gcgatgcttg tagatgacac
     7861 gggagagagg cgtcaggaac tcattcatca gcagcgtcaa ttcccgagga tccagtcctt
     7921 cggaaatgct ggtaaatcct cgcacatccg cgaacagtat ggtcatctca ctgctcttgc
     7981 cagccatgga aatactctcc ggatgcttcg ccatctcctc gaccaattct cccggcacat
     8041 actgtccgaa caaaccggtg atctgacgcc tggtgcgcga ctcgaagaag tagccgtatg
     8101 acatatccag tgccaatatc agcgagatca tcaacagact attcgccatc ggcatcacca
     8161 catcgccata ctgccaggca gccaggctca accccgcagt catggcgagc atcgacatcg
     8221 ccacaagcat gaccttgatg ggactcagca tcggcaacag aaagctcagc gtgataccga
     8281 ccaccaacaa ccaaagcact tcggcaccca gcatccaggg cgggcgcact tttaaattct
     8341 gatcgaggat gccggcaatg aggttggcgt ggatttccac cccgggaaaa acctccgcca
     8401 ccgatgtcgc ccgcatatcc atcagcccga gtgccgaagt gccgaccaaa acaatcttgt
     8461 ctttaagttg cgccggatcg atccggtcat gcagcacgtc ggcgatagat agatagcgga
     8521 aacttccctg ctctccacga tacggcacga gtgctgccac gttcgcatcc accggaattt
     8581 tgagcgtacc gctcgccgcg tccagctcca gccactccag cccggcataa ccgtctgtac
     8641 gcccctctgc gaaaccaggc ctcaatggcg gctgtcccag caatgcgcgc accatcgcca
     8701 atgacaacga ttcgtaatag gcgccgttgt attccgcgag catcggtata cgtcgcacca
     8761 caccatcact gtctaccagc ggattgaagt gtccggcgcc cgcagcggct tgctgcaact
     8821 ctggcagatt ggcgccataa ccattccagc cggtgaattc aatcggccgt cccgcgaagg
     8881 ttcctgccgg aaaagcaggc tctggcagca tcccggaaac atgagtcgcc tgctgctcgg
     8941 tcaggtaata actgagcact actggccgat ttctgatctt ctcggcgaat aatctatcgt
     9001 attcaagctg cggccggatt tgcgcgagtg cggactggaa gcttgcatcg tccctgagct
     9061 ggttctgtcc gagccgttgc aacaccccga gcccggaact ttcatccttc tcggcgaaca
     9121 ccacgtcaaa accgaccagt gccacaccat acttatcgaa cagcttgtcc atcaactcgc
     9181 cgagtcgatt gcgcccccac ggccaacgcc cctcttcctt aaggctcttt tcgtcgatgt
     9241 cgataattac gatgcgctca tccacggtgc gcggcatggt cagacgcaga cggtagtcgt
     9301 agacgatggc agagagccgg tcgacgatgc cgattcgata aaaatgggct gcgtgaccaa
     9361 caaaaaccag gacgatcaac agccccaatc ctatcaagac cgcgttcttc ttcacgccat
     9421 acccttcaga ataggcaccc agcgtccaac cccgggatca aagccgacga ccataagtcc
     9481 gacaggtcat tccttgaaat aaaattccat cttgatgcca gccagttcga tcatgtcgtg
     9541 atcctgcaac tgatgtggct ggtcacccaa ggtctgacca ttaaccagag gatgatccgc
     9601 cccttcgaca tgggtaatga aaaaaccatg cgggcggcga gtcagcaccg caacctgcac
     9661 accaggctta cccagcgtgg tcaggttctt tatcagctcc aactccttgc cggcattcgg
     9721 ccccgtcagt atttgcaccg ccgcagcctg aggcgctgcg gcagctttgg gtgcggcagc
     9781 cgtcggcacg gccgccggct gggcaggctg cagcgagcca aaacggccgg ttttctcgac
     9841 ctcttcgacc gctgcaatac gaggattcac ctgtgtctgg ctgatagcag gctcagcgga
     9901 agcg
//
LOCUS       DASUIV010000108         7883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_305699, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000108 DASUIV010000000
VERSION     DASUIV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305699"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VFW59_04000"
     CDS             complement(<1..1344)
                     /locus_tag="VFW59_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX5363405.1"
                     /translation="MRLHQITPLLAALAAILAVGATVLALDKFEREQAVHDQRLRATE
                     RVSSLRAELEATIGTATSSTRSMAVVYAANPELTANGFARLAEQAKTYSPSILNVALI
                     QNTTIRYVYPHQHNEKLVGLDFSKLPAQWPAFRNMMVSGKSSTAGPLKLVQGGEAVVV
                     RLPIYRANPDTDEDAFIGAVSAPLLISSLLRSTHLSDIEKDMQIAIRGRDGLGSRGEV
                     FYGDPGLFSADAILQHVSIPGGEWEIAARPLKGWGANTPTLNTIRLLGTLLCLLAATM
                     AYALIKHLQRRTENERRIHASEAQLQAILDNSPIAIWLVGVDGRYRFLNKTFCNAIGV
                     PESKFIAAGNLYEVMDADAAASCLQSDSACLAQEAPHRSNEVLTFVDGKRHLMEITKV
                     KLRDLTGVVSGVIGIAIDITEQRERELALKATNQAKDEFLANMSHEIRTPMNSILG"
     gene            1731..4229
                     /locus_tag="VFW59_04005"
     CDS             1731..4229
                     /locus_tag="VFW59_04005"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX5363406.1"
                     /translation="MKTPHKRTPHKYLLRSEQNTIEHTLINHLLYTVGKGSKAATGRD
                     WHDTTTHTVRDHLIERWVHTVDNYYEKDPKRLYYLSMEFLIGRMLSNAALNLGIEKSV
                     RDALRALGQDMEKVEEMETDAALGNGGLGRLAACFLDSMATLDMPGMGYGIRYEYGMF
                     NQRIEHGQQVEHPDNWLRYGNPWEFQRPERLYPVRFYGKAIRFTEASGKIEHHWTDPE
                     IVMAMAYDVPIPGYKTKTVNNLRLWVAKATREFDLDSFNAGDYIGSVEEKNSTENLSK
                     VLYPNDSSAIGRELRLKQEYFFVSASIQDILHRFDSVHKDEKGHTDWSLLPEKVAIQL
                     NDTHPAIAVAEMMYQLHDVHHLHWDRAWQMVTAIFAYTNHTLMPEALETWSVDKFEHV
                     LPRHLEIIYGINHRFLASVNHLFPGDTELLRRVSIIDEEHGRRVRMAHLAVVGSHTVN
                     GVAAIHSELLKSTLFADFHRIFPGKFINVTNGITPRRWLNQANPLLTALIESRIGGGF
                     VCDLERLSRLDEHAEDAEFRKQFRAVKYANKLRLAEYIESQIGERVDPHTLFDVQVKR
                     IHEYKRQLLNVLHVITLYNRIRCGQAAGVTPRTVIFGGKAAPGYGMAKRIIRLINDMA
                     SIINHDPAVQGLLKVVFIPNYDVSTAEKIIPACDLSEQVSTAGTEASGTGNMKMALNG
                     ALTIGTLDGANVEIRDAVGDENIFIFGLTTPEVAQLRERGYNPWDYYNGNAELRQALD
                     MIGNGFFSVQEPGRYQPICDSLLAKGDHYLLLADYASYMAMQDKVGAMYQDQEEWSRR
                     AILNVARMGKFSSDRSIREYAEKVWNVKAVPPSE"
     gene            4273..4545
                     /locus_tag="VFW59_04010"
     CDS             4273..4545
                     /locus_tag="VFW59_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363407.1"
                     /translation="MVISRDLGRKDGTDGSGGEERRVSPHLRVAFETACKVTAPFFDP
                     KQEWGGMSLTMYARQALREAYPELTQQDLALLFAAVQRFHRGALKK"
     gene            4557..6506
                     /locus_tag="VFW59_04015"
     CDS             4557..6506
                     /locus_tag="VFW59_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363408.1"
                     /translation="MPVIVYFPSSILRAVPACWLAFALWSFPCAASAASFSLSIADIT
                     SPEFSARGIVLTLPQDGSAELQIAGLRLQQRELHQVRLRCRQFKLSSAGMRCTGGAAQ
                     QLPGASLDFDYRFASQVWQFSAQLHRASAKALGALMAGMPQFTQGLLDGSVQASGEAS
                     GPNGFKADLRLTNINFGDDSGLHAAEKLSGTVRLEAARKAGVWNWRSDLVWQSGELFW
                     QPLYLSGTGERRLSASGRLDGALFSVARGEAEVAQIGKMRFSALWDGGQGKLLEASAQ
                     GDKLELTRLFADYARPFFDKGVLSEALLSGRADVDWRYRNGATRALHLALRDASFGDS
                     ARRFSLRGVNMAFDWTPDALQRVNIVFGGGELFGMPLGAGQWPVQIRGFDVDVAQAEL
                     PVLDGKLALRDFRLRREGDDWRWQFGASLSRISMERFSQAVGWPKMLGTLAGRIPKVS
                     YDGKEIAAEGALLFDVFDGSVVASGLKLVDPFGRAPRLYGNLNMRELDLDLLTRTFSF
                     GNMLGRVDVDVNNLELQDWQPARFDARIASSAGKYPKRISQKAVQNISSLGGSGAMAA
                     IQNSYLRFFESFGYDRIGWRCVLRNGVCMMGGVDDGNAGTYTIIKGGGIPAITVMGYN
                     RAVSWDELISRLKRVTQGNMKAVVK"
     gene            6548..6775
                     /locus_tag="VFW59_04020"
     CDS             6548..6775
                     /locus_tag="VFW59_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363409.1"
                     /translation="MKTNEVSFSKTNAASFAARNWIVFAGLALSACVTINIYFPAAAA
                     EKAADKIIEEVWQTQKADPQKADQPVTKEQK"
     gene            6775..7188
                     /locus_tag="VFW59_04025"
     CDS             6775..7188
                     /locus_tag="VFW59_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEX5363410.1"
                     /translation="MKTIFRSVLSLLALTFALNAFAVDLEVNTPGVNAIKQSMQARHA
                     QLAEHYASGAVGLTSDGMVSLRDAGAVPLAQRQAVSALVAAENRDRNALYGEIANANG
                     HPEWEAEIRSTFAQRWIQRAQAGWWVQTGGGWTQK"
     gene            7300..>7883
                     /locus_tag="VFW59_04030"
     CDS             7300..>7883
                     /locus_tag="VFW59_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5363411.1"
                     /translation="MNPTLVFDIETIPDIAGLRTLYELDGAVADREVAEMAFQMRRQK
                     TGGDFMQHHLQRIAAISCVLREGDNFKVWSLGEPDEDEGSLIQRFFDGVEKYTPQLVS
                     WNGGGFDLPVLHYRGLIHGIQCPRYWDMGEDDRDFKWNNYISRYHTRHLDLMDLLAMY
                     TGRANAPLDELARLIGFPGKLGMDGSKVWEAYQQG"
BASE COUNT         1558 a         2149 c         2527 g         1649 t
ORIGIN      
        1 gccgagtatg ctgttcatcg gcgtgcgtat ctcgtgactc atgttggcaa ggaactcgtc
       61 cttggcctga ttggtagcct ttagggccag ctcgcgttcg cgctgctcgg tgatgtctat
      121 cgcgatcccg atgacccccg agacgacacc ggtgaggtcg cgcaatttta ctttggtaat
      181 ctccatcaga tgccgcttcc cgtcaacgaa ggtcaacacc tcgttggacc gatgcggggc
      241 ttcctgtgcc agacacgcgc tatccgattg caggcagctc gcggcggcat ctgcatccat
      301 tacttcgtac aggttgcctg ctgcgataaa tttgctttcc ggcacaccga ttgcgttgca
      361 gaaggtcttg ttgaggaagc ggtagcgacc atccactcca accagccaga tggcgatggg
      421 cgaattgtcc agaatggcct gcaactgggc ttcgctggca tgtatgcgcc gctcgttctc
      481 ggtgcgacgt tgcagatgct tgattagtgc gtaggccatc gttgccgcga gcaggcacag
      541 caaggtgccg agcaggcgta tcgtattcag tgtcggggtg ttcgctcccc aacctttgag
      601 cggacgagcg gcgatctccc attctccgcc agggattgaa acatgttgca gaatcgcatc
      661 agcagaaaaa aggccaggat cgccgtagaa aacctcacca cggctaccga gtccgtcgcg
      721 tccgcgaatg gcgatttgca tatctttttc gatatcggaa agatgcgtgc tacgcaatag
      781 actgctgatc agtagcgggg cactcaccgc tccgatgaaa gcatcctcat ccgtgtcggg
      841 attggcccgg tagatcggga gacgcaccac cacagcctcg cctccctgca ccagtttcaa
      901 gggcccggcg gtgctggatt tgcccgagac catcatgttg cggaatgcgg gccactgcgc
      961 aggcagcttg ctgaaatcga gtccgaccag tttttcgttg tgttggtgcg ggtagacgta
     1021 gcgaatcgtc gtgttctgga tcagggcgac attgaggatg ctgggactgt aggttttggc
     1081 ctgttcggcc agacgggcaa agccatttgc cgttaattct ggattggccg catagactac
     1141 ggccatggag cgagtggatg aggtggctgt gccgatcgtg gcctcaagct ccgcgcgtag
     1201 cgaagatacc cgctcggtcg cgcgcagacg ttgatcgtga acggcttgtt ctcgctcgaa
     1261 cttgtccagt gccagaaccg ttgcaccgac cgccagtatg gcggcgagtg cggccaatag
     1321 tggcgtgatt tgatgtagtc gcatttagag cggatcctcg ttttcaagtt gcttaactga
     1381 gacgaagcat caagctggaa caaacgattc ggaaatgcca tggatagatg gcatcgcgct
     1441 gtttggagca aaatacataa tctagcctgc gcgaaaataa caacattccc gctagagcgg
     1501 tatatgtcgg aatgcctatg tatttcggca tagtcatccc cttattgcgg caggggatgt
     1561 cccagggaga ggcgtcgcct gaggcgcatc aggaaactcc aagcccgcaa gcgaaggata
     1621 aaggaataaa atcacaatgt agtttttggc tggcacggtt tgatccgttt cgattctggc
     1681 gtgctgccgg tctggttttg ttgcaatggc cgaatataga gaaggcaatc atgaagactc
     1741 ctcataaaag aacgcctcac aagtatctgc tgcgctcaga gcagaacacc atcgaacata
     1801 cgctgatcaa ccatttgctc tataccgtag gcaagggcag caaggctgcg accgggcgcg
     1861 actggcatga cacgacgacc catacggtgc gcgatcattt gatcgagcgc tgggtacata
     1921 cggtcgacaa ttactatgag aaggatccca agcggctgta ttacctgtcg atggagttcc
     1981 tgatcgggcg catgctgtcc aatgcggcgc tcaatctggg tatcgagaag tcggtgcgcg
     2041 atgccttgcg ggctctcggt caggatatgg agaaggtcga ggagatggag accgatgccg
     2101 cattgggcaa tggcggtctg gggcggttgg cggcctgttt cctcgactcg atggcgacgc
     2161 tggatatgcc cggcatgggc tacggcatcc gttatgagta cggcatgttc aatcagcgta
     2221 tcgagcacgg acagcaggtc gagcacccgg ataactggtt gcgttatggc aatccctggg
     2281 agttccagcg cccggagcgt ttgtatccgg tgcggttcta cggcaaggcg atccggttca
     2341 cggaagccag cggcaagatc gagcatcact ggaccgaccc cgagatcgtg atggcgatgg
     2401 cctatgacgt gcccattccg ggctacaaga ccaagacggt caataatctg cggctgtggg
     2461 tcgccaaggc gacgcgcgaa ttcgatctcg attcgttcaa tgcgggcgat tacatcggct
     2521 cggtcgagga gaaaaacagt accgagaatc tgtccaaggt gctttacccg aatgactctt
     2581 ccgccatcgg gcgcgagttg cggctgaaac aggaatattt tttcgtgtcg gcttcgattc
     2641 aggacatcct gcaccggttc gactcggttc ataaagatga gaaggggcat accgactgga
     2701 gtttgctgcc ggagaaggtc gcgatccagc tcaacgatac tcacccggcc atcgccgtgg
     2761 cggaaatgat gtaccagtta cacgatgtgc atcacctcca ctgggatcgg gcctggcaga
     2821 tggtcacggc gatattcgcc tataccaatc acaccctgat gccggaggcg ctggaaacct
     2881 ggtcggtgga taagtttgag catgtgctgc cgcgccatct ggaaatcatt tatgggatca
     2941 atcatcgttt tctggcctcg gtcaatcacc tgtttccggg cgacaccgag ttgctgcggc
     3001 gcgtttccat catcgacgag gagcatggcc ggcgcgtgcg catggcacat ctggcggtgg
     3061 tcggcagtca taccgtgaac ggtgtcgctg cgatccacag cgagttgttg aagagcacgc
     3121 tgttcgccga ctttcatcgc atcttccccg gcaagttcat caacgtcacc aacggcatca
     3181 cgccgcgccg ctggctgaat caggccaatc cgctgttgac cgcgctgatc gagtcgcgca
     3241 tcggcggcgg gttcgtgtgc gatctggagc gcctgtcaag actggatgaa catgcggagg
     3301 atgccgaatt ccgcaaacaa ttccgtgccg tcaaatacgc caacaagctg cgtcttgccg
     3361 agtacatcga atcccagatc ggcgagcgcg tcgatccgca tacgctgttc gatgttcagg
     3421 tgaaacgcat ccacgaatac aagcgccagt tgctcaatgt gttgcatgtc atcacgctgt
     3481 ataaccgcat tcgctgcgga caggctgcgg gcgtcacgcc ccgtacggtg atcttcggag
     3541 gcaaggcggc tcccggttac gggatggcca agcggatcat tcgcctgatc aacgacatgg
     3601 ccagcatcat caatcacgat ccggcggtgc agggcctgct caaggtggtg ttcattccca
     3661 attacgacgt ttctacggcg gagaagatca taccggcctg cgatctctcg gagcaggtct
     3721 cgacggccgg caccgaggca tcgggtaccg gcaacatgaa gatggcgctc aacggcgcac
     3781 tgaccatcgg cacgctggac ggtgccaatg tggagatacg cgatgcggta ggagatgaaa
     3841 atatcttcat cttcgggctg accacgccgg aggtggccca gctgcgcgag cgcggctata
     3901 acccgtggga ctattacaac ggcaatgccg agctgcgtca ggcgctggac atgatcggca
     3961 atggtttctt ctcggtgcaa gagccggggc gctaccagcc gatctgcgac agtttgctgg
     4021 ccaagggcga ccattacctg ttgctggccg actatgccag ctatatggcg atgcaggaca
     4081 aggtgggggc gatgtatcag gatcaggaag aatggtcgag gcgggcgatc ctcaatgtcg
     4141 cgcgtatggg caagttttcc agcgaccgtt ccatccggga atatgccgag aaggtgtgga
     4201 atgtcaaggc ggtgccaccc tcggaatagg gcgtgatgca tcgatttgaa ttgaaactta
     4261 ctggaggtgg tcatggttat ctcgagagac ttgggtcgta aggacggtac cgatggatcg
     4321 ggcggcgagg agcggcgcgt cagcccgcat ctgcgcgtcg cgttcgaaac ggcctgcaaa
     4381 gtgacggccc ccttcttcga tcccaagcag gagtggggcg gtatgtcgct gacgatgtat
     4441 gcccgtcagg ccttgcgaga ggcctatccc gagctgacgc agcaggatct cgcgctgctg
     4501 ttcgcggcag tgcagcgttt ccatcggggt gcgctgaaaa aatagcaggg agttatttgc
     4561 ccgtcattgt gtatttccca tcgtcgattc ttcgcgccgt tccggcctgc tggctggcat
     4621 ttgcgttgtg gtcgtttccc tgcgccgcca gcgcggcgag tttctcgctg agcattgccg
     4681 acatcacctc cccggagttt tccgcacgcg gcatcgtatt gaccctgccg caggatggtt
     4741 ccgccgagtt gcagatcgcc gggttgcgtc tgcagcagcg cgagttgcac caggtgcggc
     4801 tacgttgcag gcagttcaag ctttcttctg cgggcatgcg ctgtacgggg ggggcggccc
     4861 aacaattgcc gggcgcctcg ctggacttcg attatcgttt tgccagccag gtctggcagt
     4921 tctcggcgca gttgcaccgt gcctcggcca aggcgctggg cgccttgatg gcgggcatgc
     4981 cgcagttcac tcagggcttg ctggatggca gtgtgcaggc cagcggcgag gcgtcgggcc
     5041 cgaacggttt caaggctgat ttgcggttga ccaatatcaa tttcggcgat gacagcgggc
     5101 tgcatgcggc ggagaaattg agcggcactg tcaggctgga ggctgcacgc aaggcggggg
     5161 tatggaactg gcgcagcgac ttggtctggc agtccggcga gttgttctgg cagcctctct
     5221 atctgtccgg cacgggcgaa cggcggctga gtgcatcagg ccgtctggat ggcgcgctgt
     5281 tcagcgtggc gcggggcgag gccgaagtgg cgcagatcgg caagatgcgt ttttccgcgc
     5341 tttgggacgg agggcagggc aagctgctgg aagcgtcggc gcagggcgac aagctcgaac
     5401 taacaaggct gttcgccgat tacgcccggc catttttcga caagggggtt ctgtccgagg
     5461 ccttgttgtc cggtcgcgcc gatgtggact ggcgctatcg caacggcgcg acgagggcat
     5521 tgcatctggc gttgcgcgac gcctccttcg gcgacagcgc acggcgcttc tcgctgcgcg
     5581 gcgtgaatat ggcattcgac tggacgccgg atgcgttgca acgcgtgaac atcgtcttcg
     5641 gcggcggcga gttgttcggc atgcccttgg gtgcggggca atggccggtg caaatacgcg
     5701 gttttgatgt cgatgtggcg caggcggaat tgccggtgct ggacggcaag ctggcgttgc
     5761 gcgatttccg cctgcggcgc gagggcgacg actggcgctg gcaattcggc gcgtcgctga
     5821 gccgcatctc gatggagcgc ttcagtcagg cggtggggtg gcccaagatg ctgggcacgc
     5881 tggccgggcg catcccgaag gtgagctacg acggcaagga gatcgcggca gaaggcgcgc
     5941 tgttgttcga tgtgttcgac ggttcggtgg tggcgagcgg tctcaagctg gtcgatccgt
     6001 ttggccgcgc gccgcgtctg tacggcaacc tcaacatgcg ggagcttgat ctggatctgc
     6061 tgacccgcac tttttctttc ggcaatatgc tgggccgcgt cgatgtcgat gtgaacaatc
     6121 tggagctgca ggactggcag cccgcccgct tcgatgcgcg cattgccagc agcgccggga
     6181 aatatcccaa aaggatcagc caaaaggcgg tacagaacat ttcctcgctg ggtggctcgg
     6241 gggcgatggc ggcgatccag aacagttatc tgcgcttctt cgagagtttc ggctatgacc
     6301 gtatcggctg gcgctgtgtg ttgcgcaacg gcgtgtgcat gatgggaggt gtggatgacg
     6361 gcaatgcggg aacctatacc atcatcaagg gcggcggcat tccggcgatc accgtgatgg
     6421 gctacaatcg tgccgtttcg tgggatgaat tgatttcgcg gttaaagcgc gtcacgcagg
     6481 gcaatatgaa ggccgtggtc aaataagcct taaaacgatc ctctgttttt gaacctaagg
     6541 agatgtgatg aaaacgaacg aagtgagttt ctccaaaacg aacgcagcga gttttgctgc
     6601 aagaaactgg atcgtattcg cgggcctggc gctgagcgcc tgcgtcacca tcaacatcta
     6661 tttccccgca gctgcggccg agaaagcggc ggacaagatc atcgaggaag tatggcagac
     6721 ccagaaagcg gacccgcaga aggccgatca acccgtgacg aaggagcaga aataatgaag
     6781 acgattttta gatcggtact gtccctgttg gcgctcactt tcgcgctgaa tgcgtttgcg
     6841 gttgatctcg aggtcaacac gccgggcgtg aatgcgatca agcagagcat gcaggcgcgc
     6901 catgcgcagt tggccgagca ttacgccagc ggtgcggtcg gactgacctc cgacggcatg
     6961 gtctccctgc gcgatgccgg tgcggttccg ctggcgcagc gtcaggcggt gagcgctctg
     7021 gtggcggccg agaaccgcga ccgcaacgcg ctgtatggcg agatcgccaa cgccaacggg
     7081 catcccgagt gggaggcgga gatacgcagc accttcgcgc aacgctggat acagcgggca
     7141 caggccggtt ggtgggtgca gactggcggc ggctggaccc agaaataatt tctccctctc
     7201 ccggcgctga cgcgccaccc tctcctcgcg ctttggcgct cccctctccc gcatgcggga
     7261 gaggggctgg gggagagggt taatagatag tatgcaacta tgaatcccac cctcgtcttc
     7321 gacattgaaa ccatccccga catcgccggg ctgcgcacgc tgtacgagct ggacggcgcg
     7381 gtcgctgacc gggaagtggc ggagatggcg ttccagatgc gtcgccagaa gaccggcggc
     7441 gacttcatgc agcatcatct gcagcgcatt gcggccatct cctgtgtgct gcgcgagggc
     7501 gacaacttca aggtgtggtc gctgggcgaa ccggacgagg acgaaggtag cttgattcag
     7561 cgcttcttcg acggcgtcga gaaatacacc ccgcagctgg tgagctggaa cggcggcggt
     7621 ttcgatctgc cggtgctgca ctatcgcggc ctgatccacg gtatccagtg tccgcgttac
     7681 tgggacatgg gcgaggacga ccgcgacttc aagtggaaca actacatcag ccgttaccac
     7741 acgcgccatc tcgatctgat ggatttgctc gcgatgtaca ccggtcgagc caacgcgccg
     7801 ctggatgaac tggccaggct gatcggcttc cccggtaagc tgggcatgga cggcagcaag
     7861 gtgtgggagg cgtatcagca ggg
//
LOCUS       DASUIV010000109        11924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_212565, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000109 DASUIV010000000
VERSION     DASUIV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212565"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1516
                     /locus_tag="VFW59_04035"
     CDS             179..1516
                     /locus_tag="VFW59_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5363412.1"
                     /translation="MSTLPLVSHLAPDSIIAWRAEGAVTLRQFLAEVSQLAARFPAGS
                     CLLNMCSDRYRFSVGLAAAIVAEKVSLLPSTYTPEVVRQIKAFAPDVFCLTDSDQCTV
                     ELPQLRYPAMDDRSPDTFEIPRIKATQRIAVVFTSGSSGIPQPHPKNWGALVASVRAE
                     ALRLALPDHCTLIGTVPPQHMYGFESTVLMAWHSGNALSHAQPFYPADICQALATAPA
                     PRVLVSSPVHLRALLDAELALPENTRVVSATAPLPARLAFEIEARCNAPLMEIYGSTE
                     TGQIATRRPTQSAEWRLLPGIRLSLEDDGVRAYGGHIATPTIMSDMIEPINDEYFLLH
                     GRVADLVNIAGKRHSLASLNHLLNSLPGVVDGAFYMPDETDHDRITRLAACVVAPGME
                     PRSLLALLREHIDPVFLPRPLLFVEALPRNATGKLPRSALQALFQTHSAGKSA"
     gene            1513..1818
                     /locus_tag="VFW59_04040"
     CDS             1513..1818
                     /locus_tag="VFW59_04040"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEX5363413.1"
                     /translation="MNNVAHWTVPPDHPAFAGHFPGMPILPGVILLDTALQAIATATG
                     ISLDRCEIGSVKFLSPAGPGDELAIQHCISASGAIRFDITAGIRKIASGSIVPGSAA"
     gene            1815..2744
                     /locus_tag="VFW59_04045"
     CDS             1815..2744
                     /locus_tag="VFW59_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5363414.1"
                     /translation="MTSHPTSKQSAWAHTPERSNMPMLRIMTWISLRLGRRAGRVVLH
                     LIASYFLLFAPASRRASSDYLRRALGRPARWHEIYRHFFTFAATIHDRVYLVNRRFDL
                     FDFEVHGEDRLRSLLADGKGLFLIGAHLGSFEAIRAIGRKDTDLRVAMVMHEENAQKI
                     SAMLTAINPEAVQDVIGLGHIDSMLQVRERLDEGCIVGMLADRTPGNDTLHPVQILGD
                     NANLPGGPFRMAALLRRPVVFMTGLYLGGNRYAIHFETLADFSDVARGQRDAALEAAI
                     TRYAALLDHYCRKAPYNWFNFFDFWQPTTHPES"
     gene            2741..3343
                     /locus_tag="VFW59_04050"
     CDS             2741..3343
                     /locus_tag="VFW59_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HEX5363415.1"
                     /translation="MMTTPAPTRRFMRHLPAALALILLPIAAHAADWDIDQLMRGLAQ
                     NRSGHASFVEQKTIAMLNKPVESSGELLYTAPDHLEKRTLKPKAESMTVDAGTLLIER
                     GRQKHRLQLQDYPELAAFIDSIRGTLAGDRKALERSYRLSLDGTVEHWNLQLLPTDQK
                     MQAVVKHIRITGVREEVRSIEITQADGDSSVMNIEKLTPP"
     gene            3340..5823
                     /locus_tag="VFW59_04055"
     CDS             3340..5823
                     /locus_tag="VFW59_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5363416.1"
                     /translation="MSLTSARQGRTAIALWLAFILVCGIVIGRSQFTTDLSAFFPRSP
                     TPEQRLLLEQIRDGLASRLILVGIEGADAPTRARLSRQIAGQLRSDPAFVSVNNGEPI
                     NAERDRAFLFDHRYLLSPAVTPERFSAEGLHAALSESIDLLASPAGLLVKSLLPRDPS
                     GEMMQLIEQLGGGTHPRMVDGAWASRDGTRALMLMQTRASGSDTDAQQRAMAAIRQAF
                     DKTVSLSPRSLPKDCPLPNPDETTGHSTKASKNDAQVTGYLPQAGEGANVKSACFNLV
                     MTGPGVFSVTSREAIQSQVSRLSLVSVALIATLLLLVYRSVTALALGFLPVISGALAG
                     VAAVSLGFGAVHGITLGFGTALIGEAVDYSIYLFVQSEQNESDAKQQNWIRRFWPTIR
                     LGVLTSIAGFASLLLSGFPGLAQLGLYSIAGLIVAAAVTRFVLPHLLPAKFRIHDVSA
                     IGLVLSRLVQRAAKLRGVTALLLLAACAILAGHRDSLWNDQISSLSPVSQAEVALDAS
                     LRADMGASDVRYLVVASGPSRESVLQSAEQVAAILQTQVDQGELAGFESPSRYLPSTA
                     MQRARLASLPAPAVLETRLAQAAQDLPVSKELFAPFLADVDAARSQPLLQATDLAQTS
                     MAMALDALLLEQTDHWSALLPLTAPEGADIDAGRIRTALNAANLSSVLFVDMKSESDR
                     LYSGYLHEAILLSLGGLIAIVGLLLWVFRSPMHMARLLAPLAAAVVTVTAGLALFGLQ
                     LTILHLVGLLLVVAVGSNYALFFDRADCPVAPRTLASMLFANLTTVAGFGVLAFSNVS
                     ILQAMGVTVAPGVILALIYSAIFARTSRA"
     gene            5816..6613
                     /locus_tag="VFW59_04060"
     CDS             5816..6613
                     /locus_tag="VFW59_04060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5363417.1"
                     /translation="MHSWQPTPLIRATLALHGLALLAVAAAPGHWRWVLGALLANHAL
                     LTAVGLWPRSHGLGPNWTRLPAAATARNEIALTIDDGPDPVVTPQVLDMLDRYAVRAT
                     FFCIGEQAARYPDLCREIVRRGHAVENHSQHHRHHFALLGRSGFMRELQTAQDTLTAI
                     TGQRPLFFRAPAGLRNPFLDPVLARLGLRLASWSARGFDTRTGDVERVQNRVSRDLRA
                     GAILLLHDGNAARTPDGTPVILAVLPSILATARTAGLRLVTLRHALS"
     gene            6634..7314
                     /locus_tag="VFW59_04065"
     CDS             6634..7314
                     /locus_tag="VFW59_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5363418.1"
                     /translation="MNEARQPARWHQQIAAVLFSHVYLKSIGTTLFISLFFVAYFYLL
                     KNPAHPTTVMPVIFLDRLIPFQPLTLPIYLSLWLYVSLPPALLATRRELYGYGIAMTG
                     MCVAGLVVFYCWPTAIPSADIDWAQYPDVNFLKRLDASGNACPSLHVATAIFSAIWLH
                     RLLRGFGTPQWILAFNWTWCIGIVYATLSTRQHVAVDVLAGLALGILCAWLSLQHRGH
                     RRSSAPSI"
     gene            7376..8575
                     /locus_tag="VFW59_04070"
     CDS             7376..8575
                     /locus_tag="VFW59_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX5363419.1"
                     /translation="MNPLWLSHFTATSSIGRGLAPTLEALRQRRAGLAACAFDTVDLA
                     TFIGEVSGVDEVQLPHNLSDFDCRNNRLALLGLMQDGFSEAVRAAIEKYGKQRVGVFL
                     GTSTSGILQSELAYRRRDPASGALPADFIYGATHNSFSVADFTRHYFGLTGPAVVVSS
                     ACSSSAKVFSSARRMMAAGLIDAAVVGGVDSLCLTTLYGFNSLGLISEQACRPFDARR
                     NGISIGEAAAFALLERSPDHPDSNAVLLLGVGESSDAYHMSSPHPDGLGARMAMQDAL
                     KMAGLQPSEIDYINLHGTATQSNDAAEGKAVASVFGPATPCSSTKGATGHTLGAAGGL
                     EAVICALALQQGLLPAGLNTQELDPAQKLDYLLENREQPVARVLSNSFGFGGTNCSLI
                     FGRADRP"
     gene            8572..9378
                     /locus_tag="VFW59_04075"
     CDS             8572..9378
                     /locus_tag="VFW59_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX5363420.1"
                     /translation="MTFTATLTAYIEGIGLLGPGLSDWPGSRAILNGQRPYQPAKTQL
                     PSPALLPAAERRRSGAIVRLTLATGLEAIAAAGRDAASLPSVFSSSGGDGENCHAICE
                     MLASNDRQISPTRFHNSVHNAAAGYWSIATGAMATSSVLSAFDASFGAGLLEALTQVV
                     VDNSPAVLLTCDTAYPEPLYSARPIPDAFGIALVLAPLRSAQTLAQITVSLTDAGADR
                     LDDVALEALRVAIPAARGLPLLRAIALGQEQRVVLDYLDSTRLAVTVAPC"
     gene            9372..9842
                     /locus_tag="VFW59_04080"
     CDS             9372..9842
                     /locus_tag="VFW59_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HEX5363421.1"
                     /translation="MLNQTTRDHSWIARHIPHQGSMCLLDLVEAWDQQHIRCRASSHR
                     AADNPLRANGRLGCACGIEYAAQAMAVHGALLAPPDNHRAKAGYLVSVRGTKLHVPRL
                     DDIAADLRIEAVCITRSENNILYQFSVSAADKLLLEGRAAVVLDADALISGETP"
     gene            9839..10558
                     /gene="fabG"
                     /locus_tag="VFW59_04085"
     CDS             9839..10558
                     /gene="fabG"
                     /locus_tag="VFW59_04085"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX5363422.1"
                     /translation="MKNALITGGSGGIGAAICRRLAADGCHVYIHAHRGLAAAEALAD
                     EIRAGGGSAQALSFDVTNSAAVADALSPLVEQQPIQILVNNAGIHDDAVFPGMQASQW
                     HRVIDVSVNGFFNVTQPLMMPMIRSRWGRIVNVSSVAALTGNRGQVNYSAAKGALNAA
                     TKSLALEVASRGITVNAVAPGIIETPMSESAFDAQDIARMVPMKRAGTPGEVADLIGF
                     LASSQAAYITGQIISINGGII"
     gene            10573..11346
                     /locus_tag="VFW59_04090"
     CDS             10573..11346
                     /locus_tag="VFW59_04090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5363423.1"
                     /translation="MTTPETNPLVHAPHIPLTDYYQTEQDRQAYLRQIFDNTAVDYDR
                     IEAMLAFGTGPGYRREALIRAGLKKGMKVVDVGVGTGLVAAQACTITGDPALVTGVDP
                     SPGMMAASKLPKTMVLLEGRAESLPFPDNHFDFLSMGYALRHISDLSVAFAEFERVLK
                     PGGRLCILEITQAQSPLGRWLLKSYMRGVIPLLTRFISKQKNTATIWRYFWDSIEACV
                     PPEQVIATLNGVGLTQVKRHLSIGIFSEYQAIKADQPKT"
     gene            11347..>11924
                     /locus_tag="VFW59_04095"
     CDS             11347..>11924
                     /locus_tag="VFW59_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5363424.1"
                     /translation="MSTTEIFLIAMTLIFTLPYLIWRVGRTEYYAPLVVVQIITGILL
                     GPGILGAVYPEYYSFVFTPQVIQSLSGIAWWGVMIFVCIAGIELDLRKAWQHRNESVI
                     TAGLALGTPLLFGSIAAAGMLMYSGWIGPKAMTWQFVMGVGMACAVTALPILILLMEK
                     LEILRLPIGQRILRYASLDDIAIWGVLALILM"
BASE COUNT         2123 a         4008 c         3534 g         2259 t
ORIGIN      
        1 ccacctggtc ggttttcgcc aatttactga cactgccgtt ggtcgcaatg atgttcgtgg
       61 ccgaatatct ggtacgcaga aagaccctcc ccgatcttga tcaaacacac atcctcgatg
      121 cggtgcgggc gttcagaaac actccgatac atccacgcta gtcgttaccg acccgaccat
      181 gtcgacactg cctctggtat cgcacctcgc cccggacagc atcatcgcct ggcgcgctga
      241 gggcgccgtc acgctgcgcc agtttctggc cgaggtcagc cagcttgccg cccgctttcc
      301 tgcggggagc tgcctgctca acatgtgcag cgaccgttac cgttttagcg tgggtctggc
      361 ggcggccatc gtggccgaaa aggtcagcct gctgccgtcc acctacacgc cggaagtggt
      421 gcgccagatc aaggcttttg caccggatgt gttttgtctg accgacagcg accagtgcac
      481 ggtggagctg ccccagttgc gctacccggc gatggatgac cgctcgccgg acacctttga
      541 aatcccccgg atcaaggcca cgcagcgcat cgccgtggtc tttacttcag gctcgagcgg
      601 catcccgcaa ccgcacccga aaaactgggg cgcgctggtc gccagtgtgc gggccgaggc
      661 cttgcgcctg gcgctgccgg accactgcac cctgatcggc accgtgccgc cgcaacacat
      721 gtacggcttc gaatccacgg tactgatggc atggcacagc ggcaacgcgc tcagccatgc
      781 tcagccgttt tatccggccg acatctgtca ggcgctggcg actgcccccg cgccccgcgt
      841 gctggtatcc tcgcccgtgc atctgcgcgc gctgctggat gcggagctgg cgctgcccga
      901 gaacacccgg gtggtgtccg ccactgcgcc gctgcccgcc cggctcgcat tcgaaatcga
      961 ggcgcgttgc aatgcgccgc tgatggagat atacggcagc accgagaccg ggcagatcgc
     1021 gacccgccgc cccacgcaaa gtgcggaatg gcggctgctt cccggaatca ggctcagtct
     1081 ggaggacgat ggcgtgcgcg cttacggcgg tcacatcgct accccgacca tcatgagcga
     1141 catgatcgaa ccgattaacg acgagtattt cttgctgcat ggacgcgtcg ccgatctggt
     1201 caatatcgcc ggcaaacgcc actcgctggc cagtctcaat cacctgctca acagcctgcc
     1261 cggcgtggtg gatggcgctt tttacatgcc ggacgagacc gaccatgacc gcatcacccg
     1321 gcttgccgcc tgcgtcgtgg ctccgggcat ggagccacgc tctctgctgg ccttgctgcg
     1381 cgaacatatc gacccggtct tcctgccgcg cccgctgctg ttcgtcgagg ccctgccgcg
     1441 caacgccacc ggaaaactgc cgcgctcggc gctgcaggcc ctgtttcaaa cacacagcgc
     1501 aggaaaatcc gcatgaacaa tgtcgcgcac tggacggttc cgccggatca tcccgccttt
     1561 gccggacact ttcccggcat gcccattctg cccggcgtga tactgctgga tacggcacta
     1621 caggcaatcg ccacagccac cggaatctcg ctggatcgct gcgagatcgg ctcggtcaaa
     1681 ttcctgagcc cggcgggccc gggcgacgaa ctggcgatcc agcactgtat ttccgccagt
     1741 ggagcgatac gtttcgacat cacggccggg atacgcaaga tcgccagcgg cagcatcgta
     1801 ccgggatctg ccgcgtgacc tcgcacccga caagcaagca atccgcctgg gctcacaccc
     1861 ctgagcgcag caacatgccg atgttgcgca tcatgacctg gatttcactg cgtctgggac
     1921 ggcgcgccgg gcgcgtggta ctgcatctga tcgccagtta ttttctgctg ttcgcaccgg
     1981 ccagccgccg tgcttcaagc gactatctca ggcgcgcgct agggcgacct gcccgctggc
     2041 acgagatcta ccggcatttt ttcacctttg ccgccaccat ccatgaccgc gtctatctgg
     2101 tcaaccgccg cttcgacctg ttcgacttcg aggtgcatgg cgaggatagg ctgcgcagcc
     2161 tgctggccga cggcaagggc ctgttcctga tcggcgcgca tctgggcagc tttgaagcga
     2221 tccgcgcgat cggacggaaa gacaccgacc tgcgcgtcgc catggtcatg cacgaggaga
     2281 acgcgcaaaa gatcagcgcg atgctcacgg ccatcaaccc ggaggccgta caggatgtca
     2341 tcggcctggg ccacatcgac tccatgctcc aggtgcgcga gcgtctggac gagggctgca
     2401 tcgtcggcat gctggcagac cgcacccccg gcaacgacac cctgcacccc gtgcagattc
     2461 tgggcgataa cgcgaacctg cccggcgggc ctttccgaat ggccgcactg ctgcggcgtc
     2521 cggtggtatt catgaccggg ctgtatctgg gcggcaaccg ttacgccatc cacttcgaga
     2581 cgctggcgga tttttccgat gtggcgcgcg ggcagcgcga tgccgcgctg gaagcggcga
     2641 tcacccgtta tgccgcactg ctggatcatt actgccgcaa ggcaccgtac aactggttta
     2701 atttcttcga tttttggcaa cccaccacgc atccagaatc atgatgacga cacccgcacc
     2761 aacacgccgc ttcatgcggc acctgcccgc agcattggca ctgatactgt tacccatcgc
     2821 cgctcacgcc gccgactggg acatcgatca gctgatgcgc ggtctggcac agaatcgctc
     2881 cggtcacgcc agctttgtcg agcagaaaac catcgccatg ctcaacaagc cggtggagtc
     2941 ttccggcgaa ctgctctaca ccgcgcccga ccatctggaa aaacgcaccc tcaaacccaa
     3001 agccgaatcc atgacggtgg atgccggcac gctgctgatc gagcgcggtc gccagaaaca
     3061 tcggctgcaa ttgcaggatt accctgaact ggctgctttc atcgacagca tccgaggcac
     3121 gctggccggt gaccgcaagg cgctggaacg cagttatcgc ctgagtctgg atggcaccgt
     3181 cgagcactgg aacctgcaac ttctgcctac cgatcaaaaa atgcaggcgg tggtcaaaca
     3241 cattcgcatc accggtgtac gcgaagaagt gcgcagcatc gaaattaccc aggccgacgg
     3301 cgacagctcg gtgatgaaca tcgaaaagtt gaccccgccg tgagcctgac gtcggcacgg
     3361 caaggccgga cagcgatcgc cttatggctg gcgtttatcc tcgtctgcgg catcgtcatc
     3421 ggccgcagcc agttcaccac ggatctgtcc gcattctttc cgcgcagccc cacccccgag
     3481 cagcgcctgc tgctggaaca gatacgcgac ggactggctt cgcgcctgat tctggtgggc
     3541 atcgaaggcg ccgatgcgcc cacccgcgcc cggctttcca gacagatcgc cgggcagctg
     3601 cgcagcgatc cggcctttgt cagcgtcaat aacggcgagc ccatcaatgc cgaacgcgac
     3661 cgtgcttttc tgttcgacca tcgctacctg ctcagccctg cggtaacgcc cgaacgcttc
     3721 agtgcagagg gcctgcatgc cgccctgagc gagagcatag acctgctcgc ctcgccggcc
     3781 gggctgctgg tcaagtcgct gctgccgcgc gacccgagcg gcgaaatgat gcaattgatc
     3841 gaacagctgg gcggcggcac ccacccacgg atggtcgatg gcgcatgggc ctcgcgcgac
     3901 ggcacccgcg ccctgatgct gatgcaaacg cgcgcctccg gctccgatac cgatgcccag
     3961 caacgcgcca tggcagccat acggcaagcg ttcgacaaga ctgtttctct ctcccctcgc
     4021 tctctcccaa aagactgccc tctccccaac cctgatgaaa ccactggcca ttcgactaag
     4081 gcatcaaaaa acgatgccca agtcactggt tatctcccgc aagcgggaga gggggcaaac
     4141 gtaaaaagcg cttgttttaa tttggtgatg accggccccg gcgtattttc ggtgacttcg
     4201 cgcgaggcga tccagagtca ggtcagccgc ctgtccctcg tcagcgtggc gctgatcgcg
     4261 acgctgctgt tgctggtcta tcgttccgtc accgcgctgg cattggggtt tctgccggtc
     4321 atcagcggtg cgctggcggg cgtcgccgcc gtcagtctgg gctttggcgc ggtgcatggc
     4381 atcacgctgg ggttcggcac cgcactgatc ggcgaggcgg tggattattc gatctacctg
     4441 ttcgtgcaat cggagcaaaa cgaatccgac gcgaagcagc aaaactggat ccggcgcttc
     4501 tggcccacca tccgcctcgg cgtactgacc tcgatagccg gcttcgcctc gctgctgctc
     4561 tccggctttc ccggactggc gcaactcggg ctgtattcga tagcgggact gatcgtcgcc
     4621 gcagcggtga cccgcttcgt cttgccccat ctgctgccgg cgaagttccg catacacgat
     4681 gtctcggcca tcggcctcgt gctgtcccgt ctggtgcagc gcgcagcaaa attgcgcggg
     4741 gtgacagcgc tcctgctgct ggcggcctgt gccatcctgg ccggacatcg cgacagtctg
     4801 tggaacgacc agatatcctc gttaagcccg gtatcacagg ccgaggtggc gctggacgcc
     4861 agcctgcgcg ccgacatggg ggcatccgat gtgcgctatc tggtcgtggc gtccggcccg
     4921 agccgggaat ccgtgttgca gtctgccgaa caggtcgccg cgatactgca aacacaagtc
     4981 gatcagggcg aactcgccgg gtttgagagt cccagccgct accttcccag caccgcgatg
     5041 cagcgcgccc ggctggccag cctgcccgca cccgccgtgc tggaaacccg tctggcgcaa
     5101 gccgcccagg acttgccggt gagcaaggaa ctgttcgcac cgttcctcgc cgatgtcgat
     5161 gccgcgcgca gccagccctt gctgcaagcg accgatcttg cacagacctc gatggccatg
     5221 gcgctggatg ccttgctcct tgagcagaca gatcactgga gcgccctgtt gccgctgacc
     5281 gcccccgagg gggccgacat cgacgctggc cggatccgca cggcgttgaa cgcagcgaac
     5341 ctgtccagtg tgctgttcgt ggatatgaaa tccgagtccg accgcctgta ttccggctac
     5401 ctgcatgaag ccatcctgtt gtcgctgggc gggctgatcg cgatcgtcgg cttgctgctg
     5461 tgggtgtttc gctcgcccat gcacatggct cgcctcctcg cgccactcgc ggccgcagtc
     5521 gtcaccgtca cggcggggct ggccttgttc ggtctgcaac tgaccatcct gcatctggtc
     5581 ggtttgctgc tggtggtcgc ggtgggatcg aattacgccc tgttcttcga ccgggccgac
     5641 tgccccgtcg caccgcgcac gctcgcctcg atgctgtttg ccaatttgac gactgtcgcc
     5701 ggtttcggcg tgctggcgtt ttccaacgta tcgatcttgc aggccatggg cgtcaccgtg
     5761 gcgccgggcg tcatccttgc gctgatctac tcggcgattt tcgccagaac atcccgtgca
     5821 tagctggcaa ccgacaccgc tgatccgcgc caccctcgcg ctgcacgggc tggccttgct
     5881 ggccgtagcc gccgcacccg gacactggcg ctgggtgctg ggcgcattgc tggccaatca
     5941 cgcgctgctc accgcagtcg gactgtggcc gcgcagccac ggactgggcc cgaactggac
     6001 gcggctgcct gccgccgcca cggccagaaa cgaaatcgcc ctgaccatag acgacgggcc
     6061 ggatcccgtc gtcactccgc aggtgctcga catgctggat cgttatgcgg tgcgggcgac
     6121 cttcttctgc atcggcgaac aagccgcacg ctacccagac ctgtgccgtg agatcgtgcg
     6181 ccggggtcat gcggtggaaa accacagcca gcatcaccgc catcattttg cgctgctggg
     6241 gcgttccggt ttcatgcgtg aactgcagac ggcgcaggac accctcaccg caatcaccgg
     6301 ccagcgcccc ctgttctttc gcgcccccgc cggcttgcgc aatccttttc tcgacccggt
     6361 gctcgcccga ctcggactgc gtcttgccag ctggtcggca cggggtttcg acacccggac
     6421 cggtgacgtt gagcgcgtgc agaaccgggt gtcgcgcgac ctgagagcgg gggccatttt
     6481 actgttgcac gatggcaatg cggcccgcac gcccgacggc accccggtca tcctcgccgt
     6541 gctcccctcg attctggcca ccgcgcgcac agccggtctg cgtctcgtca ccttgcggca
     6601 cgccctgtca tgacccgccg cgacgaagct gccatgaacg aagcgcgtca gcctgcgcgc
     6661 tggcatcagc aaatcgccgc cgtcctgttc agccatgtgt atctcaagag tatcggcacg
     6721 acgctgttca tcagcctgtt tttcgtcgct tacttttacc tgctcaaaaa tccggcccat
     6781 ccgaccacgg tgatgcccgt catctttctg gatcgcctga tcccttttca gccgctgacc
     6841 ttgcccatct acctgtcgct gtggctctat gtctccctgc ccccggcatt gctggcgacg
     6901 cggcgcgagt tgtacggcta cggcatcgcc atgaccggaa tgtgcgttgc cggactggtc
     6961 gtcttctatt gctggcccac cgccatacca tcggccgata tcgactgggc ccagtatccc
     7021 gacgtgaact tcctcaagcg cctggatgcc tccggaaatg cctgcccttc gctgcatgtc
     7081 gccaccgcca ttttttcggc tatctggctg catcgcctgc tgcgcggctt cggcacgccg
     7141 caatggatac tcgccttcaa ttggacatgg tgtatcggca tcgtctatgc caccctgtcc
     7201 acgcgccagc atgtcgctgt ggatgtgctg gccggcctcg cgctgggcat actgtgcgcc
     7261 tggctgtcat tgcaacatcg aggacatcgg cgatccagcg cgccctcgat ttgatccgaa
     7321 caggctaccg gaatttgtta gactcacgct taatttacaa aaggtttatt tttctttgaa
     7381 tccgctctgg ctctcccatt tcaccgcgac cagcagtatc ggccgcggtc tcgccccgac
     7441 cctggaggca ctgcgccagc gccgcgccgg actggcggcc tgcgcgttcg acacggtgga
     7501 tctggcgact ttcatcggcg aagtgagcgg agtggacgag gtgcagcttc cccacaacct
     7561 gtccgatttc gattgccgca acaaccggct cgcgttgctg ggattgatgc aggatggctt
     7621 ttccgaagcg gtgcgggccg ccatcgaaaa atacggcaaa cagcgcgttg gcgtgttcct
     7681 cggcaccagc acttccggaa ttctgcaaag cgagctggct taccggcgac gcgatccggc
     7741 aagcggcgct ctgcccgccg attttatcta tggcgcgacg cacaacagct tttccgtcgc
     7801 ggatttcacg cgccattatt ttggcctgac cggccctgcg gtcgtggtgt cgtcggcctg
     7861 ttcgtccagc gccaaggtgt tcagttcggc acgacgcatg atggcggcag gattgatcga
     7921 tgcggcggtg gtcggcggcg tggactcgct gtgcctgacg acgctgtatg gcttcaactc
     7981 gctcggcctg atttccgaac aggcctgccg tcccttcgat gcgcgacgca acggcatctc
     8041 gatcggcgag gcggcggcct tcgcgctgct ggaacgcagc ccggatcatc cggacagcaa
     8101 tgccgtgctg ctgctcggcg ttggcgaatc cagcgacgcc tatcacatgt catcgccgca
     8161 cccggacgga ctgggtgcgc gcatggcgat gcaggatgcg ctgaagatgg cgggtttgca
     8221 gccatccgaa atcgactata tcaacctgca cggcaccgcc acccagagca acgatgccgc
     8281 agaaggaaag gccgtcgcat ccgtattcgg cccggccacg ccgtgcagct ccaccaaggg
     8341 cgcgaccgga cacacgctgg gcgctgccgg cggtctggag gcggtgatct gcgcgctggc
     8401 gctgcaacaa ggcttgctgc ccgccggatt gaacacccaa gaactcgatc ccgcacagaa
     8461 gctcgactat ctgctggaga atcgcgaaca gccggtggcg cgcgtgctca gcaattcgtt
     8521 tggcttcggc ggcaccaatt gcagcctgat ctttggacgg gcggacaggc catgacattc
     8581 accgcgacac tcaccgccta catcgaaggc atcggcctgc tcggccccgg tttaagcgac
     8641 tggcccggca gccgggcgat cctcaacgga cagcggccct atcagccggc caagacccag
     8701 ctgccctcac ccgcgttgct gcctgcggca gaacgccggc gcagcggcgc catcgtcaga
     8761 ctcacgctgg cgaccggact ggaagcgatt gcggctgccg gacgggatgc cgccagtttg
     8821 cccagcgtgt tctcatcctc cggcggcgac ggggaaaact gtcacgccat ctgcgagatg
     8881 ctcgcatcaa atgaccggca aatctccccg acccgcttcc acaattcggt gcacaacgcg
     8941 gccgccggat actggagcat cgccaccggc gcgatggcca cctcatcggt gttgagtgcc
     9001 tttgacgcca gcttcggcgc gggactactg gaagcgctaa ctcaggtggt cgtggataac
     9061 agccctgccg ttctgctgac ctgcgacacc gcctacccgg agccgctgta cagcgcccgc
     9121 cccatccccg atgcattcgg catcgcgctg gtgctggcac ctctgcgcag cgcacagacg
     9181 ctggcgcaga tcaccgtcag cctgactgat gccggcgcgg accggctcga cgatgttgcg
     9241 cttgaagccc tgcgcgtcgc cattcccgcc gcgcgcggat tgccgctgct gcgcgcaatc
     9301 gcgctagggc aggaacaacg cgtggtgctg gattacctgg acagcacgcg tctggccgtg
     9361 accgtcgcac catgctgaat cagaccacac gggatcacag ctggatcgcc cggcacatcc
     9421 cgcatcaggg cagcatgtgc ctgctcgatc tcgttgaggc atgggatcag cagcacatcc
     9481 ggtgtcgcgc gagcagccac cgcgccgccg acaatcctct gcgcgccaac ggccgactgg
     9541 gttgcgcctg cggtatcgaa tacgcggcac aggccatggc cgtgcacggc gccttgctgg
     9601 caccgccgga taatcaccgc gccaaggccg ggtatctggt cagcgtgcgc gggaccaagc
     9661 tgcatgtgcc ccggctggac gacatcgccg ccgatctgcg aatcgaagcc gtctgcatca
     9721 cgcgcagcga aaacaacatc ctctaccagt tcagcgtcag cgcggcggac aagctgctgc
     9781 tggaggggcg cgccgcagtc gtgctcgatg ccgacgcact catatcagga gaaacgccat
     9841 gaagaacgcc ctgatcaccg gcggcagcgg cggcatcggc gcggccatct gccgccgtct
     9901 ggccgccgat ggctgccatg tctacatcca tgcgcatcgc ggactggccg ccgcagaagc
     9961 gctggccgat gagattcgtg cgggcggcgg cagcgcccag gcgctgtcat ttgacgtgac
    10021 caacagcgcc gcagtcgccg atgcgctgtc cccgctcgtc gaacagcagc cgatccagat
    10081 tctggtcaac aatgccggca tccacgacga tgccgtgttc cccggcatgc aggcctcgca
    10141 atggcaccgg gtgatcgacg tgtccgtcaa cggcttcttc aatgtcaccc agccgctgat
    10201 gatgccgatg atccgcagcc gctgggggcg catcgtcaac gtatcttccg tcgccgcgct
    10261 gaccggcaac cgagggcagg tcaattattc cgcagccaag ggcgcgctca acgccgccac
    10321 caaatcgctc gcgctcgaag tcgccagccg gggcatcacc gtcaatgcgg tcgcacccgg
    10381 catcatcgaa accccgatga gcgaatccgc gttcgacgca caggacatcg cacgcatggt
    10441 gccgatgaaa cgcgcaggca ctcccggcga agtggccgac ctgatcggct ttctcgcctc
    10501 atcccaggct gcctatatca ccgggcagat catttccatc aacggcggca taatttaaca
    10561 gtgagtgacc ctatgactac ccctgaaaca aatcctttgg tgcatgcacc gcacattccg
    10621 ctgaccgatt actaccagac agaacaggac aggcaggctt atctgcgcca gattttcgac
    10681 aatactgcgg tggattacga ccgtatcgag gcgatgctgg cattcggcac cggccccggc
    10741 taccggcgcg aggcgctgat acgcgccggc ttgaaaaagg gcatgaaggt cgtcgatgtg
    10801 ggcgtgggca cgggactggt tgcggcgcaa gcctgcacga tcaccggcga ccctgcgctg
    10861 gtcaccggag tcgatcccag ccccggcatg atggccgcca gcaagctgcc caagaccatg
    10921 gtgctgctgg aaggccgcgc cgaatccctg cccttccccg acaaccattt tgatttcctg
    10981 agcatgggct atgcgctgcg ccacatcagc gatctttccg tggccttcgc ggaattcgaa
    11041 cgggtgctga aaccgggcgg ccgcctgtgc atactcgaaa ttacccaggc gcaaagccca
    11101 ttgggacgct ggttgctcaa gagctacatg cgcggcgtga taccgctgct gacaagattc
    11161 atctcgaaac agaaaaacac cgcaacgatc tggcgctact tctgggacag catcgaggcc
    11221 tgtgtcccgc ccgaacaagt catcgccaca ctgaacggcg tgggactgac gcaggttaaa
    11281 cgccatctga gcatcggtat cttttcggaa tatcaggcga tcaaggctga ccagccgaaa
    11341 acataaatga gtaccaccga aatattcctg attgcgatga cactgatctt cacccttccc
    11401 tatttgatct ggagggtggg gaggacggag tactacgccc cgctggtggt ggtgcagatc
    11461 atcaccggca tcctgctcgg ccccggcatt ctcggtgcgg tttatcccga gtactactcc
    11521 ttcgtattca cgccgcaagt catccagtct ttgagcggta tcgcatggtg gggcgtgatg
    11581 atcttcgtgt gcatcgccgg tatcgaactc gatctgcgta aagcatggca gcaccgcaac
    11641 gaaagtgtga tcacggccgg cctggcactc ggcacgccgc tgctgttcgg ctctatcgcg
    11701 gcagcaggca tgttgatgta tagcggctgg atcggcccca aggcgatgac ttggcaattc
    11761 gtgatgggtg tgggcatggc ctgcgcggtc accgcgctgc cgattctgat cttgctgatg
    11821 gaaaaactgg aaatcctgag actacccatc ggtcagcgca tcctgcgtta tgccagtctg
    11881 gacgacatcg ccatctgggg cgtgctcgcg ctgatcctga tgga
//
LOCUS       DASUIV010000110         7054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_399011, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000110 DASUIV010000000
VERSION     DASUIV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399011"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..1132
                     /gene="modA"
                     /locus_tag="VFW59_04100"
     CDS             368..1132
                     /gene="modA"
                     /locus_tag="VFW59_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5363425.1"
                     /translation="MDISIGRAVVAGLLASLVLASAPVRAGEVRVAVASNFAAPMERI
                     VASFQAESGHRVSVSTGSTGKFYAQIKNGAPFDVFVAADSATPQRLEEDGVTVRGSRF
                     VYALGKLALWSAQPGVVDAKGAVLRSGYNKLAIAEPKLAPYGMAAKETLEKLGLWNGV
                     QARLVRGENITQTYQFVASGNAELGLVALSQITREGKITAGSSWVVPPHLYRPIQQSA
                     VQLSAAKDPLAAKAFLDFMRGKQAAAVMRSFGYELP"
     gene            1219..1905
                     /gene="modB"
                     /locus_tag="VFW59_04105"
     CDS             1219..1905
                     /gene="modB"
                     /locus_tag="VFW59_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX5363426.1"
                     /translation="MTALTPDDLQAVWLTLKLASVTTLLLLCLATPLAWWLAHSSKWY
                     KGMVAALVALPLVLPPTVLGFYLLLLMGPQGVVGELTHALHLGALPFTFAGLVIGSVI
                     FSLPFAVQPIQNAFEAMGKRPLEVAATLRAHGWDRFFSVALPLARPGFLTAIILAFAH
                     TVGEFGVVLMLGGSIPGETQVLSVSIYSHVEAMEYGSAHLLAGGMVLFSFMVLLGLYL
                     AGGKLAKVKP"
     gene            1902..2975
                     /gene="modC"
                     /locus_tag="VFW59_04110"
     CDS             1902..2975
                     /gene="modC"
                     /locus_tag="VFW59_04110"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEX5363427.1"
                     /translation="MSELHAHFKLQLEGFALDASFSAPAVGVTALFGPSGSGKTTLLR
                     CIAGLERAQGTLRVNGAIWQDETTFMPTHQRPLGYVFQEASLFPHLSVRANLEYGYKR
                     IAPAGRKVQLDRVVEWLGLGRLIERGDPARLSGGERQRVAIGRALLTSPRILLMDEPL
                     SALDTASKQDILPYLERLHRELNIPVLYVSHALDEVARLADHLVLLQQGRVIASGGLG
                     ETLARLDLPTAHFDDAGSVIEAAVAQQDETYHLTRVDYAGGHLWVGRVAQPSGARVRA
                     RVLARDVSIATQAPEGSSINNILHARIEEIRDEGPDKVMVRMKVGESQMLLSRITRRS
                     RDHLNLAVGMDVFAQVKSVALMS"
     gene            3069..3395
                     /locus_tag="VFW59_04115"
     CDS             3069..3395
                     /locus_tag="VFW59_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363428.1"
                     /translation="MARRNHYHVYVIELSKEVLREGKFMRCNPDYIPGKPCVYVGMTG
                     LDPDLRFDKHKAGIQSNRYVMQYGLRLRPDLYEVYNPMPYDGARDMEVELAIGLQEEG
                     YGVWQA"
     gene            3475..3996
                     /locus_tag="VFW59_04120"
     CDS             3475..3996
                     /locus_tag="VFW59_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HEX5363429.1"
                     /translation="MRPKPPPNYLAGYPAALVEQVRRLIEQDRLADWLLQKYPQAHGI
                     RTDSALYDYVQELKGEYLRNAAQLSRVAFDGKLQSIGQALGTHTRIARVQGGKLKTKR
                     ELRVATVFKEMPPEFLRMIVVHELAHLKELEHNKAFYQLCLHIEPDYHQLELDLRAYL
                     CHLDATGRALWAA"
     gene            complement(4027..4524)
                     /locus_tag="VFW59_04125"
     CDS             complement(4027..4524)
                     /locus_tag="VFW59_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363430.1"
                     /translation="MYPVHDIDAILLLALSRASKRRPAELVDILAAADLDQGVTPNEI
                     KMIDSFYRLSEYGLIREVDGGYTLTEEGEQITSHRRKNSVPLKRIFEIKERLADFNLN
                     GEHAVILLDAKKLSAAVLEHRAAKNSDEKNLLVAKPKPKPVEFNSKRPPQRFPAKKHK
                     HKPKG"
     gene            complement(4594..6009)
                     /gene="dbpA"
                     /locus_tag="VFW59_04130"
     CDS             complement(4594..6009)
                     /gene="dbpA"
                     /locus_tag="VFW59_04130"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEX5363431.1"
                     /translation="MTNMNTSSPSAENHTGRSFSELPLSPAMLANLQQLGYLTMTPIQ
                     SASLPIALGGFDLIAQAKTGSGKTAAFALPLLTNLNPRFFAVQAMVLCPTRELADQVS
                     QELRRLARAADNIKIITLCGGSTMRPQMASLEHGAHIVVGTPGRIMDHLERGTLNLEA
                     LNTLVLDEADRMLDMGFYDQIAYVAKQCPAQRQTLLFSATYPDGIARLARQFLRKPQE
                     VKLTEQHDDSKIRQRFYEVSREQRLPAVAALLRHYRPVSTLAFCNTKQQCRDLLDLLH
                     AQGFHALTLNGDMEQRERDQVLIQFANRSCSVLVATDVAARGLDIAQLEAVINVDVTP
                     DPEVHVHRIGRTGRADENGWALSLASPSEIHRVETIAKMMGFEPEWHELGELQGSDAP
                     LLPPMVTLQILGGRKEKIRPGDVLGALTGDAGYTREQVGKINVTDFSTYVAVARNIAD
                     EAVRKLSAGKVKGKTVKVRAL"
     gene            6447..>7054
                     /locus_tag="VFW59_04135"
     CDS             6447..>7054
                     /locus_tag="VFW59_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEX5363432.1"
                     /translation="MTNMKVGTRLGLSFGAVTLLIIIVVLMAWFRLEAIESRWQDFGK
                     VTLMKKSAIMDGYINLGNGVHHFKNYILRGGDYNTKFMQDMDALDKIEQAYQATGVVS
                     AEEQKLIQVIKEGTQDYRNGMNQLVKLKASGADMTQLDKAVAGADKALAAALSQFFKL
                     TEDNVQAETEGISALVVLTQEIMGVSGVLAIILSIILAMLITR"
BASE COUNT         1284 a         1982 c         2328 g         1460 t
ORIGIN      
        1 ctcccacaag ggcagcgggt tttcgtagga gcggcttaag ccgcgaaggt ggcggggagg
       61 tgaaacgtgt cctatcgcgg ctctcgtgcc ctttagggtg aaaggctgtc ctgatccttc
      121 gacaagctca ggactaaagg aaagtcgaag ggccgctccc acagtcattg caggatgggt
      181 taaacaaaac gggcacatgg gtgcccgttt tgcttgagtg gattcagttt ttccttaact
      241 ttgcgtctca gaagaagttc cgtcgccaat gccggaatgt agccgaacaa gccatccgat
      301 ccggataatc cgggccggtc ttcatcttgc ttgttatcat ggcggcgatt tcggagaggg
      361 ggaggacatg gatatttcga tagggcgtgc tgtcgtggcg ggtctgttgg ccagtctggt
      421 gttggcgagt gcgccggtgc gggcgggcga ggtgcgcgtg gcggtggcgt cgaacttcgc
      481 tgcacccatg gagcgcatcg ttgcgtcgtt tcaggcagaa agcggccatc gggtgagcgt
      541 cagtaccggt tcgaccggaa agttctacgc gcagatcaaa aatggtgcgc cgttcgatgt
      601 gttcgtggcg gcagattcgg caacgccgca acgtctggaa gaagacgggg tgacggttcg
      661 cggttcgcgt ttcgtctatg ccttgggcaa gctggcgctg tggagtgcgc agcccggcgt
      721 ggtggatgcc aagggtgcgg tgttgcgcag cggctacaac aagctggcga ttgccgagcc
      781 gaagctcgct ccctacggca tggcggcgaa agagacgctg gaaaaactcg ggctgtggaa
      841 cggggtgcaa gccaggctgg tgaggggcga gaacatcacg cagacttacc agttcgtcgc
      901 cagcggtaat gccgaattgg ggctggtcgc gctgtcgcag atcacgagag agggcaagat
      961 aacggcaggc tcgtcgtggg tggtgccgcc gcatctgtat cgtccgatcc agcagagtgc
     1021 cgtgcagttg tctgccgcga aagatccgct tgcggccaaa gccttcctcg acttcatgcg
     1081 gggcaagcag gcggcagcgg tgatgcgcag tttcggttac gaacttcctt gagtagaagc
     1141 ggctgtccgc agatttcgca gattgcgaaa tctctgacat gaataatctg ttgagttttt
     1201 cagactggaa ttttcctcat gactgcttta acccccgatg acctgcaagc cgtctggctg
     1261 acgctcaagc tggcgagcgt caccacgctg ctgctgctgt gcctcgccac tccgctggcc
     1321 tggtggctgg cgcacagcag caagtggtac aaggggatgg tggccgcgct ggtggccttg
     1381 ccgctggtgt tgccgccgac ggtgctgggt ttttatctgc tgctgctgat ggggccgcag
     1441 ggcgtggtgg gcgaactcac ccatgccctg catctcggcg cgctgccgtt cacctttgcc
     1501 ggactggtga tcggttcggt gatcttctcg ctgccgtttg cggtgcagcc gatccagaat
     1561 gccttcgagg cgatgggaaa acgtccgctg gaagtggccg ctaccctgcg tgcccatggc
     1621 tgggatcgtt ttttcagtgt ggccttgccg ctggcgcgcc ccggctttct caccgcgatc
     1681 atcctcgcct tcgcccatac ggtgggcgag ttcggcgtgg tgctgatgct gggcggcagc
     1741 atcccgggcg agacgcaggt gctctcggtc tccatctaca gtcacgtcga ggcgatggaa
     1801 tacggctcgg cccatctgtt ggcgggcggc atggtgctgt tctcgttcat ggtgttgctc
     1861 gggctgtatc tggcgggcgg caagctggcg aaggtgaagc catgagcgag ttgcacgcgc
     1921 actttaaact gcagctggag ggcttcgcgc tcgatgcgtc cttcagcgcc cccgccgtcg
     1981 gcgtcaccgc gctgttcggc ccgtccggtt cgggcaagac caccttgctg cgctgtatcg
     2041 ccgggctgga gcgcgcgcag ggcacgctgc gggtgaacgg cgcaatctgg caggacgaga
     2101 cgacctttat gcccacccat cagcgccccc tgggctatgt gttccaggag gcgagcctgt
     2161 ttccgcatct gtcggtgcgc gccaatctgg aatacggcta taagcgcatc gcgcccgccg
     2221 ggcgaaaagt gcagttggat cgggtggtgg aatggctggg gctggggcgc ctgatcgaac
     2281 gcggcgatcc ggccagactc tccggcggcg aacgccagcg cgtcgccatc ggccgcgcgc
     2341 tgctcaccag tccgcgcatt ctgctgatgg acgagccgct gtccgcgctc gacaccgcca
     2401 gcaagcagga catcctgccg tatctggagc ggctgcatcg cgagctgaac atcccggtgc
     2461 tgtatgtcag ccatgcgctg gacgaggtgg cgcggctggc cgaccatctg gtgttgttgc
     2521 aacaggggcg cgtcatcgcc agcggcggac tcggcgagac gctggcgcgg ctggatctgc
     2581 ccaccgcgca tttcgacgat gcgggcagcg tgatcgaggc ggcggtggcg cagcaggacg
     2641 agacctatca tctgacgcga gtggattacg caggcggtca tctgtgggtg ggcagggtgg
     2701 ctcagccgtc cggcgcccgc gtgcgcgccc gggtgctggc ccgcgacgtg agcatcgcca
     2761 cgcaagcgcc ggaaggctcc agcatcaata atattttgca tgcccgcatc gaagagatac
     2821 gcgacgaggg gccggacaag gtgatggtgc gcatgaaggt gggcgagtcg cagatgctgc
     2881 tgtcgcgcat cacgcgccgc tcgcgcgacc atctgaacct tgccgtgggc atggacgtgt
     2941 tcgcgcaggt gaagagcgtc gccttgatgt catgacccaa aataatcggc cgcatccggc
     3001 aagccgcaac ccatgacgag ccccatgtcc tgacatgagc cccgttcaat accgctatca
     3061 aaacaagcat ggcacggcgt aaccattacc acgtctacgt gatcgaactg tcgaaagaag
     3121 tccttcgcga agggaaattc atgagatgca accccgatta cattccgggc aaaccgtgcg
     3181 tctatgtcgg catgaccgga ctggatccgg acttgcgctt cgacaaacac aaggcaggca
     3241 tccagtccaa ccgctatgtc atgcaatacg gactgcggct gcgccccgat ctctacgagg
     3301 tctacaaccc gatgccctac gacggcgcac gcgacatgga agtggagctg gcgatcggtt
     3361 tgcaggaaga gggctatggc gtctggcagg cctagccggt cccgattcgg caggatgggg
     3421 gcgccgacaa ccggtagaat cccgccttcc tgcactcgtt gatatccgtt ccccatgcgt
     3481 cccaagccac cgcctaacta tcttgccggt tatcccgctg cactggtcga gcaggtgcgg
     3541 cggctgatcg agcaggacag gctggcagac tggttgctgc aaaagtatcc gcaggcgcat
     3601 ggcatccgca ccgacagtgc gctgtatgac tacgttcagg aactgaaagg cgagtatctg
     3661 cgcaacgcgg cgcagttgag ccgggtggcg ttcgacggca agttgcagtc gatcgggcag
     3721 gcgctgggta cgcacacccg catcgcgcgg gtgcagggcg gcaagctcaa gaccaaacgc
     3781 gagctgcgcg tggcgacggt gttcaaggag atgccgccgg aattcctgcg catgatcgtg
     3841 gtgcacgagc tggcccatct caaggaactg gagcacaaca aggccttcta tcagttatgc
     3901 ctgcatatcg agcccgacta tcaccagctg gagctggacc tgcgggccta cctctgccat
     3961 ctcgatgcga cggggcgtgc gctgtgggcg gcatagcgcc cactttctac tccccactca
     4021 ctactttcag cctttaggct tgtgtttgtg cttcttcgcc gggaagcgtt gcggcgggcg
     4081 tttgctgttg aactccaccg gtttgggttt cggtttggct accagcaaat ttttctcgtc
     4141 gctgtttttg gctgcccggt gttccagtac ggcggcgctg agttttttgg catccagcag
     4201 gatgacggcg tgttcgccgt tcaggttgaa gtcggcaagg cgctccttga tctcgaagat
     4261 gcgcttgagc ggaaccgagt tcttgcgccg gtgggaggtg atctgctcac cctcttcggt
     4321 gagcgtatag ccgccgtcga cctcgcggat caatccgtat tccgacaggc ggtagaacga
     4381 atcgatcatc tttatttcgt tgggcgtgac gccctgatcg agatcggcgg cggcgaggat
     4441 gtcgaccagc tccgccggac gccgcttgga agcgcgcgac agggcgagta aaagaatggc
     4501 atcaatatcg tggactgggt acatcgtgaa cctctgaata aatatgggcc aacaggccaa
     4561 agttgcaatc ttgcaggtgg atccgggtga gtcttacagc gcgcgcacct tcacggtctt
     4621 gcccttcacc ttgcctgccg acagtttgcg cacggcttca tcggcgatgt tgcgtgcgac
     4681 ggcgacatag gtggagaagt cggtcacatt gatcttgccg acctgttcgc gagtgtagcc
     4741 cgcatcgccg gtgagcgcgc ccagcacgtc gccgggacgg atcttctcct tgcggccgcc
     4801 gaggatctgc agcgtgacca tcggcggcag cagcggtgcg tcgctgcctt gcagctcgcc
     4861 cagctcgtgc cactcgggtt cgaaacccat catcttggcg atggtctcga cgcggtggat
     4921 ctcggacggg ctggcgagac tcagcgccca gccattctca tcggcgcgac cggtgcgtcc
     4981 gatgcggtgg acatgcacct cggggtcggg cgtgacatcg acgttgatga ccgcttcgag
     5041 ctgcgcgatg tccaggcccc gcgccgcgac gtcggtcgcc accagcaccg aacagctgcg
     5101 gttggcgaac tggatcagca cctgatcgcg ttcgcgctgt tccatatcgc cgttcagcgt
     5161 cagcgcatgg aagccctgtg cgtgcagcag gtcgagcagg tcgcggcatt gctgcttggt
     5221 gttgcagaag gccagcgtgc tgaccgggcg gtaatgcctg agcagggcgg cgacggcggg
     5281 cagacgttgt tcgcggctca cctcgtagaa gcgctggcgg atcttgctgt cgtcgtgctg
     5341 ctcggtcagc ttcacttcct gaggtttgcg caggaactgg cgcgccagcc gggcgatgcc
     5401 gtctgggtag gtggccgaga acagcagggt ctggcgctgt gccggacact gcttcgccac
     5461 gtaggcgatc tggtcgtaga aacccatgtc cagcatgcga tcggcctcgt ccagcaccag
     5521 cgtattgagc gcttccagat tcagcgtacc gcgttcgagg tgatccatga tgcgccccgg
     5581 cgtgccgacg acgatgtgcg cgccgtgttc cagactggcc atctgcggac gcatggtgct
     5641 gccgccgcac agcgtgatga tcttgatgtt gtcggccgcg cgcgccagac ggcgcagttc
     5701 ctgagacacc tgatccgcca gctcgcgcgt ggggcacaac accatcgcct gtaccgcaaa
     5761 aaagcgcggg ttcaggttgg tcagcagggg cagtgcgaac gccgcagtct tgccgctgcc
     5821 ggttttggcc tgtgcgatca ggtcgaagcc gccaagcgcg atcggcaggc tggccgactg
     5881 gatcggcgtc atggtcagat agccgagctg ttgcaagttg gccagcattg cgggcgacag
     5941 cggcagttcg ctgaacgagc gtccggtatg gttttctgcg gagggggagg aggtgttcat
     6001 gttggtcatg cgcaattatc ccagtctgcg gctagtcgtg tgcagctaaa tttgtactgg
     6061 gtctatttgc cgggcgatgg aggcggtagg caagagtgtt gcacatttcc agcggtgtcg
     6121 ttttccgcat aagcgacggc acgtaaaatt cggttccgca ccaattggga ggcaggtaag
     6181 ttgctggatt gtggcgcgac cattttcttt atgtcccata aagttatcag aatcacctga
     6241 taccccccct tgattcgctt gtatatagtc tgacgtgatg gcgctaaggc cgcgccggaa
     6301 ttcggcggaa cgatggatgt ccgcgatacc atccgctggg cagggtggat gaacggatga
     6361 tcatccgtcg tcgatatcga gaagctgatg accagtcgcg acatggaact ggttgaaatg
     6421 gcggttgcat aacagggaga gttgtcatga caaacatgaa agttggtact aggctgggat
     6481 tgagtttcgg tgcagtcacg ctgttgatca tcatcgtcgt gctgatggcc tggttcaggc
     6541 ttgaagcgat cgagagtcgt tggcaggact tcgggaaagt caccctgatg aagaagagtg
     6601 ccatcatgga cgggtatatc aatctcggca acggcgtgca ccacttcaag aactacattt
     6661 tgcggggggg ggattacaac accaagttca tgcaggacat ggatgccctc gacaagattg
     6721 agcaggccta tcaggctacc ggcgttgtct ctgcagaaga gcagaagctg attcaggtga
     6781 tcaaggaggg cacgcaggac tatcgcaatg gcatgaacca gctggttaaa ctgaaggcca
     6841 gcggtgcgga catgacccaa ctcgacaagg cggtggcagg tgccgacaag gcgcttgcgg
     6901 cggcattgtc gcaattcttc aagctcaccg aggacaatgt tcaggccgag acggagggta
     6961 tttctgcgtt ggtggtgctg acccaggaaa ttatgggcgt gtcgggcgtg ctggctatca
     7021 tcctgtcgat tattctggca atgctgatca cgcg
//
LOCUS       DASUIV010000111         3138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1328435, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000111 DASUIV010000000
VERSION     DASUIV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328435"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFW59_04140"
     CDS             complement(<1..183)
                     /locus_tag="VFW59_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363433.1"
                     /translation="MNEVRVFAIVLIVAGCLGLAYGGFTYTKDTHEAKLGPIELSVKE
                     TETVNIPVWLGLAAVAA"
     gene            complement(199..345)
                     /locus_tag="VFW59_04145"
     CDS             complement(199..345)
                     /locus_tag="VFW59_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX5363434.1"
                     /translation="MLETIAILLLIMWALGLVTAYTMGGLIHLLLVIAIVVILIRVIQ
                     GRRL"
     gene            complement(387..698)
                     /locus_tag="VFW59_04150"
     CDS             complement(387..698)
                     /locus_tag="VFW59_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5363435.1"
                     /translation="MKQFKYISSIFLAVMLAAVLGCASSPKQEGTGEYIDDSVVTTKV
                     KAAVFNEPSLKSAEINVETFKGVVQLSGFVNSTADIQKAVEIARGVKGVTSVKNDMRV
                     K"
     gene            1195..2484
                     /gene="cls"
                     /locus_tag="VFW59_04155"
     CDS             1195..2484
                     /gene="cls"
                     /locus_tag="VFW59_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX5363436.1"
                     /translation="MAMQPQHPVQLDGAHGPLTARQSKAVLARLKKNSDDTNIFDKHL
                     AVEAEIVGSPLVVGNKVELLVDGPTTYDAMFDAIEKAKDNVNMETFIIEDDEIGRRFV
                     ELLSRKQRSGVQVNLIYDSVGAINTPRAFFNPLKESGVNILEFNPINPLHARKGWQFN
                     HRDHRKLLIVDGRIAFVGGINISSVYSSGSFGRSRPSKGSQPWRDTHLRMAGPVVGEF
                     QKLFIATWAQQKGDPLAPRTYFPEPGTMGNEVVRAIGSSPEEPYSQIYATLLSAINSA
                     ETRVLLTNAYFVPDPQLLAALKEAVQRGVDVQLLLPGKTDSMLVFYASRSYYDELLSA
                     GVKIHERQDALLHAKTALVDGVWSTVGSTNLDWRSFVNNQEINAVVLGQDFGAQMQAM
                     FDKDLESSSPVTLEAWRQRSLGARIKERLARLWAHWL"
     gene            complement(2500..>3138)
                     /locus_tag="VFW59_04160"
     CDS             complement(2500..>3138)
                     /locus_tag="VFW59_04160"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5363437.1"
                     /translation="LGGRVLLPKGNNTMRSLLLLLALMAPCGVGRADAVSPEAMVKNT
                     ANEVMEILKSDKDAGNGDMHKLGDLVEEKIASKFNFNRISKMILGRYWNTASQEQQDR
                     FIVEFRSLLVRTYSSALSKYRNQTIEYKPLRSAPGDTSVKVRTQIIQPGGPAIPLDYS
                     LENTDGNWKVYDVNIDGLSLVTIYRGQFAEELKQNGVGGVIQRLAEKNRHLP"
BASE COUNT          723 a          895 c          812 g          708 t
ORIGIN      
        1 cgccgcaacg gccgccaggc ctaaccagac cgggatattc actgtctccg tctccttgac
       61 cgacagctcg atcgggccca gttttgcctc atgcgtgtcc ttggtgtagg taaaaccgcc
      121 atacgccaac cccaggcaac cggccacgat cagcacgatt gcaaaaaccc tgacttcgtt
      181 catcgcagtt ctcccggatt acaaacgtcg cccctgaatc acgcgaatca gtatcacgac
      241 gatcgcaatc accagaagca aatgaatcaa gccacccatg gtgtatgcag tcaccaggcc
      301 caacgcccac atgatcagca atagaatcgc aattgtttcg agcatgttca ttcactcctt
      361 aaaaggccgg tcgtatcgac aagctgtcat ttgacccgca tgtcgttctt gacggatgtc
      421 acccctttta cgccacgcgc aatctcgacg gctttctgga tatccgcagt ggaattgacg
      481 aagccgctta actggacgac gcccttgaag gtttcgacgt tgatttcggc agacttgagc
      541 gaaggttcat taaacaccgc cgctttaacc ttcgtcgtga caacgctgtc gtcaatgtat
      601 tctcccgtgc cttcctgctt ggggctggat gcgcaaccaa gcacggcagc aagcatgacg
      661 gcgaggaaaa tgctggaaat gtatttaaat tgtttcatgg ctttattctc caaatgaatt
      721 gaaccgtatc cacgatcaaa ggtgtcgccg tagatgtctg ttagcctttg tatccatcgg
      781 ctagcccaac gactacgatg ctccgctcta tttcgatacc aatctgttcg ataacgcaca
      841 tatcgctcgc gtttttctga agcttggctg ttaatcatct cgacgatccc gaccgcgatt
      901 cctctcttgc tcccggtcgg attccggtgt cagaaatcag cctgatttgc gcatgctagc
      961 tgcgcccata acgacactct gttcggtagc gaacagaggt tcatcgacaa tcttgttagc
     1021 ctgtccaatg gatagtcgcc atgtgtcact gcgggtataa ttcgaatgaa gatgtgggaa
     1081 ataccgatgg gccgagggga aatccggagt gcccctatcg caagattggc ggctatctgt
     1141 tgcctggcag ttttgccggg cggttgcggc tccatcccca ccatggtgcc cgatatggcg
     1201 atgcagccac agcatcctgt gcagctggat ggtgctcacg gcccgctgac cgcaagacaa
     1261 agcaaggccg tcctcgccag gctcaagaaa aatagcgacg acaccaatat tttcgacaaa
     1321 catctggccg tggaggcgga gattgtcggc agcccgctgg tagtcggcaa caaggtggag
     1381 ctactggtcg acggcccgac aacctacgat gccatgttcg atgctatcga aaaggcaaaa
     1441 gataacgtca atatggaaac cttcatcatc gaggatgacg agatcgggcg gcgctttgtc
     1501 gagctattga gccgcaaaca gcgcagcggc gtacaggtca acctgattta cgatagcgtt
     1561 ggtgccatca atacacccag ggcattcttc aatccgctga aggaaagcgg cgtgaatatc
     1621 ctcgaattca accccatcaa tccgctgcat gcgcgcaaag gctggcaatt caaccatcgc
     1681 gatcatcgca agctgttgat cgtggatggg cgaatagcct ttgtcggcgg catcaacatc
     1741 agcagcgtgt attccagcgg atcgttcggc cgatcgagac cgagcaaagg cagccagccg
     1801 tggcgcgata cccatttacg catggcgggc ccggtggtcg gcgaatttca gaagctgttc
     1861 atcgctacat gggcccagca aaaaggcgac ccgttggccc ccagaaccta tttccccgag
     1921 cccggcacca tgggcaacga ggttgtgcgt gccatcggca gttcgcccga agagccctac
     1981 agccagattt atgccaccct gctctccgcc atcaatagcg ccgaaacccg ggttctgctg
     2041 accaacgctt atttcgtgcc cgacccgcaa ctgctcgccg cactgaagga agccgtgcag
     2101 cgcggcgtgg acgtacaact gttattacct ggaaaaacag actcgatgct ggtgttttac
     2161 gcttcgcgtt cgtattacga cgagttgttg agcgccgggg taaaaatcca tgaacggcag
     2221 gatgccttgc tgcatgcgaa aaccgcactg gtcgatggcg tatggtccac cgtaggttca
     2281 accaatctgg attggcgtag cttcgtcaac aatcaggaga tcaacgctgt tgttctgggg
     2341 caagatttcg gcgcacaaat gcaggccatg ttcgataagg atctcgaatc atcaagcccg
     2401 gtaacgctgg aagcatggag acaacgctcg cttggtgccc gcatcaagga aaggctggcg
     2461 cgcttgtggg cacactggct gtaaccccca tgatcgcggt caaggcaagt gccggttctt
     2521 ttccgcgagc cgctggataa cgccaccgac cccgttctgc ttcagttctt cggcaaactg
     2581 tccgcggtat atggtcacca gacttaagcc gtcgatattc acgtcataga ctttccagtt
     2641 gccgtcggtg ttttccaggc tgtaatccag cggaatggca ggaccgccag gctgaataat
     2701 ctgcgtcctg accttgactg aagtgtcgcc gggcgcagag cgcaagggct tgtattcaat
     2761 cgtctgattt cggtactttg acagcgccga tgaataggtg cgcaccagca aggaacggaa
     2821 ttcgacgatg aaccgatcct gctgctcctg gctcgcggta ttccaatagc gtccaagtat
     2881 catcttggag atgcggttga aattaaactt gctcgcgatt ttctcttcga ccagatcgcc
     2941 gagcttatgc atatccccgt tcccggcatc cttgtccgac ttgagaattt ccatgacctc
     3001 attggccgta tttttcacca tggcttccgg cgaaacggca tcggctctgc ccacaccgca
     3061 aggtgccatg agtgccagca ataacaagag cgatctcatg gtgttattac ccttgggcag
     3121 caagacacgg ccacccaa
//
LOCUS       DASUIV010000112         7656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_770890, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000112 DASUIV010000000
VERSION     DASUIV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770890"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /locus_tag="VFW59_04165"
     CDS             complement(<1..2261)
                     /locus_tag="VFW59_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX5363438.1"
                     /translation="MNNTEAKAAVADTHRIPLAELERRLSCTGGGLGRAEAALRLSRY
                     GSNELVVRKETPEFIKFLRQFTNFFALLLIAGSALAFFADYLKPGEGNAYIGVALLVV
                     VLLNALFTYVQEYESERIMESFRNMLPQMIAVLRDGIVLRIEARLLVPGDVILLEEGD
                     RVPADGRLIEVNQLKVDHSSLTGESEPQLRKLACTHDNLLESRNMVFSGTLVQSGNGR
                     ALVYGTGMNTQIGRIVQLTKEIDAIETPIRKELRHFIRIISGIAIALGVLFFLLSVLL
                     GNPLIGSLIFAIGIIVANVPEGLLPTVTLALTMASKRMAKKKALIRNLEAIETLGSTT
                     VICTDKTGTITQNQMKVSTLVLGEREWSAYQHGLCQAQGFDEAWAAMTLCNNARLTDH
                     GYQGDPTEGAMLVFAGQMKPLADLFARFPRIHENPFDSATKRMITTHRAGNGQPNLAY
                     MKGAPEVVFTECTQSLRQGVLVEFDAAARQRALETYERLAARGERVLALAYKTTGAPE
                     AEEADFIYLGLVGMLDPPRTETAGAIAKCRTAGIRVFMITGDYQVTAESIARQVGLFT
                     GDGRVVVGVELQQMSDEELSVLLDSRELVFARSSPMQKLRIVKALQNKGEIVTVTGDG
                     VNDAPALKNADMGVAMGLSGTEVAKEAADMVLMDDNFATIVNAVEEGRTIFANIKKFL
                     AYVLTSNIPELFPFIAFVVIDAPLALTVVLILSIDLGTDLLPALGLGRVPPEHDVMKQ
                     PPRRRDERLLSWPL"
     gene            complement(2332..3141)
                     /locus_tag="VFW59_04170"
     CDS             complement(2332..3141)
                     /locus_tag="VFW59_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5363439.1"
                     /translation="MAIVPTAGAASLPPATPRPDHIVIVVEENKSYSQIIGNPVAPYV
                     NELSRRGVLFTQSYGVTHPSQPNYLALFSGSTRGIGSNACPLELHGENLASALIDKGL
                     SFVSYAESLPQPGFAGCIYGAYYRKHNPVANWTEHAALNQPFSALPQDYAQLPTVALV
                     VPDQRNDMHDGSIAQGDDWLARNIEPYAQWAMTHNSLLIVTWDEDDGWSNNRVATIMV
                     GPMVKPGSSAQRINHYTLLRMIGEMYGLPYLGESAHEKSIAGVWQVRGKRP"
     gene            3308..3592
                     /locus_tag="VFW59_04175"
     CDS             3308..3592
                     /locus_tag="VFW59_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5363440.1"
                     /translation="MNRKELIDALAEKTGSTKVDADRNIAALIEIVTATLKKGDNVAL
                     VGFGTFEVRKRAARTGRNPATGAELKIKASKVPAFKAGANLKAAVNGVKK"
     gene            complement(3694..4209)
                     /locus_tag="VFW59_04180"
     CDS             complement(3694..4209)
                     /locus_tag="VFW59_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEX5363441.1"
                     /translation="MANKAPSRARLWRRRAIEALLIVAVVAGIRAWQQRDVASGAAPM
                     LSGALQDGRRYTLPFHPAKPVLVHFWASWCPVCRAEQGSIQSMAQDHPEVITVAMQSG
                     SAVEVAKHLDEQGLNFPTLNDADGRISAAWGVHAVPASFIIDRDGQVRFVEIGYTTGP
                     GLRLRLWWAGL"
     gene            complement(4385..4786)
                     /locus_tag="VFW59_04185"
     CDS             complement(4385..4786)
                     /locus_tag="VFW59_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX5363442.1"
                     /translation="MSHMHHDAGERLRIDKWLWAARFFKTRALAAEAVESGKVLINEA
                     RVKPAKTVAIGDWLELHIGQYSYRVEVLGLSGKRGPASEAQQLYRESDESRLRREMIA
                     AQNRLLPQPLLKGRPTKRDRREIERFNVKFT"
     gene            complement(4793..5221)
                     /locus_tag="VFW59_04190"
     CDS             complement(4793..5221)
                     /locus_tag="VFW59_04190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5363443.1"
                     /translation="MGNPFTVSLVSWHDGEPLLRAVREAVFMQEQGVPEELEWDGLDE
                     GCRHALALSLQGEAIGCGRILANGHIGRIAVLPSWRGQHVGTALMEALLDEARAQGFH
                     QVEVDAQTQALPFYHSLDFVEQGEVFMDAGLPHIKMKLAL"
     gene            complement(5380..5859)
                     /locus_tag="VFW59_04195"
     CDS             complement(5380..5859)
                     /locus_tag="VFW59_04195"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5363444.1"
                     /translation="MKITVDIPTQTLELRDDAGELLRRYSVSTASHGAGERAGSNCTP
                     RGRHVVRAKIGAGQPENAVFVGRRPTGEVYTPELGAQFPQRDWILTRILWLSGCEPGF
                     NRLGECDTMRRYIYIHGTPDQQGMGVPASHGCIRMGNADLIELFDLVPAGTVVEINA"
     gene            complement(6012..6521)
                     /gene="tadA"
                     /locus_tag="VFW59_04200"
     CDS             complement(6012..6521)
                     /gene="tadA"
                     /locus_tag="VFW59_04200"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX5363445.1"
                     /translation="MTDQAIFSSVIAPAEQSDDAFMRAALELARQAQAAGEVPVGAVV
                     VKDGEIVGRGYNAPISRHDPSAHAEMQALRDAAQHLGNYRLVGCELYVTLEPCLMCAG
                     AIMHARIARLVYGARDLKTGACGSVLDAFAEPRLNHHTEVAGGVLAEECSATLSRFFA
                     MRRAQQRSA"
     gene            6622..7098
                     /locus_tag="VFW59_04205"
     CDS             6622..7098
                     /locus_tag="VFW59_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5363446.1"
                     /translation="MSIKEIDNLLLRFISEVELFRNIDLDDVAALLRQSTRIDVARDE
                     LVFEEGDQGYSMYIVTRGVFEVYRSVAGQHVHIARITPGEHFGEIALLSNRPRSASVR
                     ATLPSTVIRLSKNALLSEQRAAVQLFRNMALLMAQRLLSVEDEIILHKTGRHPSQQ"
     gene            complement(7129..>7656)
                     /locus_tag="VFW59_04210"
     CDS             complement(7129..>7656)
                     /locus_tag="VFW59_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HEX5363447.1"
                     /translation="VKDVEFERREIIVREGKGNKDRVTVLPENLILPLQAHLVKVKAL
                     HERDLDAGFGEVYLPNALAAKYPKAARTWGWQFVFPSAVRSTDPRSGIERRHHLYEAS
                     VQRAVREAAKIAEIHKPVTPHVLRHSFATHLLQSGYDIRTVQELLGHSDVSTTMIYTH
                     VLNKGGRGVVSPLDR"
BASE COUNT         1497 a         2515 c         2207 g         1437 t
ORIGIN      
        1 agcggccagc tgagcagcct ttcatcgcgc ctgcgcggcg gctgtttcat cacgtcatgc
       61 tcgggcggta cgcgtcccag ccccagagcc ggcaacagat cggtacccag atcgatgctc
      121 agtatcagca cgaccgtcag cgccagcggc gcgtcgatga ccacgaaggc gataaaggga
      181 aacagctcgg ggatattgct ggtcagcaca taggcgagga atttcttgat attggcaaag
      241 atggtgcgtc cttcctcgac ggcgttgacg atggtggcga agttgtcgtc catcagcacc
      301 atgtccgccg cctccttggc cacttcggtg ccggacagcc ccatcgccac gcccatgtcc
      361 gcatttttaa gcgcaggcgc atcgttgacg ccatcgccgg tgaccgtgac gatctctcct
      421 ttattctgca gcgccttgac gatgcgcagc ttctgcatcg gcgacgagcg cgcgaagacc
      481 agctcgcggc tgtccagcag aaccgacaat tcttcgtcgc tcatctgctg caattccacg
      541 cccacgacca ccctcccgtc gccggtgaac agcccgacct gccgggcgat ggattcggcg
      601 gtgacctgat agtcgccggt gatcatgaac acccggatac ccgcagtccg gcacttggca
      661 atcgcaccgg cggtctccgt gcggggcgga tccagcatgc cgaccagccc cagatagatg
      721 aaatccgcct cctcggcttc gggcgcaccg gtggtcttgt atgccagcgc gagcacgcgt
      781 tctccgcgcg cggcgagacg ctcgtaagtc tccagcgcgc gttgccgtgc cgccgcatcg
      841 aattcgacca gcaccccctg ccgcagactc tgcgtgcatt cggtgaagac gacttccggc
      901 gcccccttca tgtaagcgag gttaggctgg ccgttccctg cgcggtgcgt ggtgatcatg
      961 cgcttggtgg cggagtcgaa gggattttca tggatacgcg gaaagcgggc gaacaaatcc
     1021 gcgagcggtt tcatctgacc ggcgaacacc agcatcgcgc cctcggtcgg atcgccctga
     1081 taaccgtggt cggtgaggcg tgcgttattg cacagggtca tcgcggccca ggcctcgtca
     1141 aaaccctggg cctggcacag gccgtgctga taggccgacc attcgcgctc gcccagcacc
     1201 agcgtggaga ccttcatctg gttctgggtg atggttccgg tcttgtcggt gcagatcacc
     1261 gtggtcgaac ccagcgtctc gatggcttcc agattcctga tcagcgcttt tttcttcgcc
     1321 atgcgtttcg aggccatggt cagcgccagc gtcacggtgg gcaacagacc ttccggcacg
     1381 ttcgcgacga tgatgccgat cgcaaagatc agactgccga tcagcgggtt gccgagcagc
     1441 acgctgagca ggaaaaacag cacgcccagc gcgatggcga taccggaaat gatgcggatg
     1501 aaatgccgca actctttgcg tatcggcgtt tcgatcgcat cgatttcctt ggtgagctgg
     1561 acgatgcgtc cgatctgggt gttcatgccc gtgccgtaaa ccagcgcgcg gccgttgccg
     1621 ctttgcacca gcgtgccgga aaacaccatg ttgcggcttt ccagcaggtt gtcgtgagtg
     1681 caggccagct tgcgcagttg cggctcgctc tcgccggtca gcgaggaatg atcgaccttg
     1741 agctggttga cttcgatcag gcgcccgtcg gcgggaacac ggtcgccctc ttccagcagg
     1801 atcacatcgc ccggcaccag cagcctggcc tcgatgcgca acacaatacc gtcgcgcaac
     1861 acggcgatca tttgcggcag catgttgcgg aagctctcca tgatgcgctc gctctcgtac
     1921 tcctgaacgt aggtgaacag cgcattgagc aacacgacca cgagcagggc cacgccgata
     1981 taggcattgc cttcgcccgg cttcaggtaa tcggcgaaga acgccagcgc ggaacccgcg
     2041 atgagcagca gcgcgaaaaa attggtgaac tggcgcagaa acttgatgaa ttcgggcgtc
     2101 tctttgcgca ccacgagttc gttgctgccg taccgggaca gacggagggc cgcctcggca
     2161 cggcccagtc cgccgccggt acagctcaag cgccgttcca gctccgcaag ggggatccgg
     2221 tgcgtatcgg ctaccgccgc ttttgcttcg gtgttattca tgccatccct tgctgaacag
     2281 attcagaaag cgcatgcatc gagtggcctg tcgtctcggc acaccggcgc cctaaggtct
     2341 tttgccacgc acctgccaca ccccggcgat cgatttctca tgcgcgctct cgcccaaata
     2401 aggcaggcca tacatctcgc cgatcatgcg cagcagggtg taatgattga tgcgttgcgc
     2461 actgctgccc ggcttgacca tcggcccgac cataatcgtg gcgacgcggt tgttcgacca
     2521 gccgtcatcc tcatcccagg tgacgatcag cagactgttg tgtgtcatcg cccactgcgc
     2581 ataaggctcg atgttgcgcg ccagccagtc atccccctgc gcgatgctgc cgtcgtgcat
     2641 atcgttgcgc tgatcgggca cgaccagcgc caccgtcggc aattgcgcat agtcctgcgg
     2701 caaggccgag aacggctgat tgagcgcggc atgctcagtc cagttcgcca ccgggttgtg
     2761 cttgcggtaa tacgcgccat agatgcaccc cgcaaaaccc ggctgcggca gggattcggc
     2821 gtagctgacg aaactcagtc ccttgtcgat cagcgcactc gccagattct cgccgtgcag
     2881 ctccagcgga caggcattgc tgccgatgcc gcgcgtgctg ccggaaaaca gcgcgagata
     2941 attgggctga ctgggatgcg tgacgccgta ggattgggtg aacagcacac cgcgacggga
     3001 cagctcgtta acatagggcg ccaccgggtt gccgatgatc tgcgagtagg acttgttctc
     3061 ctccacgacg atgacgatat gatcgggacg cggcgtcgcg gggggaaggg aggcggcacc
     3121 ggcggtgggt acaattgcca tccagagcat aaaaacaggg cgtttcatga cgctcctcgg
     3181 gtaaaaatta cgaaaaatag cgattacggg cacaatactt caacttaccc cttgccatcc
     3241 agccaaatat ccacaacaat ccgcaacggc ttttaaccaa aaccacaacc acaaggagca
     3301 atcgcaaatg aaccgtaaag aactgatcga cgcactggca gagaagaccg gcagcaccaa
     3361 ggtagatgcg gatcgcaata tcgcagccct gatcgaaatc gttacagcca ctctgaaaaa
     3421 gggtgacaac gtggcgctgg tcggcttcgg caccttcgaa gtgcgtaaac gcgctgcacg
     3481 taccggccgc aacccggcaa ccggcgcaga gctgaagatc aaggcttcca aggttccagc
     3541 attcaaggct ggcgcgaacc tgaaagctgc agtgaacggc gtcaagaagt aattaacgcc
     3601 ctgcactcca aaaggccaag ggcagagccc ttggcctttt tttattgacg ctagaataat
     3661 ttccggcgct tctttaccaa gcctgcacga cagtcacagc cccgcccacc acagccgcaa
     3721 gcgcagtccg ggcccggtgg tataaccgat ctcgacgaag cgaacctgtc cgtcgcggtc
     3781 gatgatgaaa ctggcgggta cggcatgcac tccccaggcg gcggagatgc gaccatccgc
     3841 atcgttcagc gtcggaaaat tcagcccctg ctcatcgaga tgtttcgcga cttcgacagc
     3901 gctaccgctt tgcattgcca ccgtgatgac ctcgggatga tcctgcgcca tggactggat
     3961 gctgccctgc tctgcgcggc acaccggaca ccagctggcc cagaaatgca ccagcaccgg
     4021 cttggccgga tggaatggca gcgtgtagcg gcggccatcc tgcaacgccc ccgacagcat
     4081 cggcgccgcg ccgctggcca catcgcgctg ctgccatgcg cggatgcccg cgacgacggc
     4141 gacgatcagc agcgcctcga tcgcgcgacg gcgccacagc ctggctcgtg atggcgcttt
     4201 attggccatc acgagccctg cctgacggac acggcaactg tgacgcctct ggtaataaaa
     4261 attgccatgc ccgtttccct ctcctcaatc ctctgatcaa acgactagcc attcgactaa
     4321 gctggcaaac aacaccagca agtcgctggt tatcccgcaa acgggcgagg aagcaaacga
     4381 atcgctacgt gaacttcaca ttaaaccgtt cgatttcgcg gcggtcgcgc ttggtcggcc
     4441 tgcccttgag cagcggctgc ggcagcagcc tgttctgggc ggcaatcatt tcgcggcgca
     4501 accggctctc gtcgctctcg cgatacagct gttgcgcctc cgatgccggg ccgcgcttgc
     4561 cggacaggcc cagcacttcg acccggtatg aatactggcc gatatgcagc tccagccagt
     4621 cgcctatcgc caccgtcttg gccggtttga cgcgcgcctc attgatcagc accttgccgc
     4681 tctcgaccgc ctcggccgcc agcgcgcgcg tcttgaagaa gcgcgcggcc cacagccatt
     4741 tgtcgatgcg gagtctttcg ccggcgtcgt gatgcatgtg tgacatgacg cgctacaacg
     4801 ccagcttcat cttgatatgc ggcagcccgg catccatgaa cacctcgccc tgctcgacga
     4861 aatccagact gtgatagaac ggcagcgcct gcgtctgcgc gtccacctcc acctgatgaa
     4921 aaccttgcgc acgcgcctcg tcgagcaggg cttccatcag cgccgtgccg acatgctgcc
     4981 cccgccatga cggcagcacc gcgatgcggc cgatatgacc gttcgccagt atccgcccgc
     5041 agccgatggc ctcgccctgc aggctcagcg ccagcgcgtg gcggcagcct tcgtccagac
     5101 cgtcccattc cagctcctcg gggacgccct gctcttgcat aaacacggcc tcgcgtaccg
     5161 cgcgcaacag cggttcgccg tcgtgccagg acaccagact gacggtaaaa ggattgccca
     5221 tcgtgcacct cccgtacatt tcgaaaacct actgcgcacc tcgattgctg cgttgcgcgg
     5281 tgctcgaaat cctcatgtac attaagtaca ttccggtttc tgcgctccgt gcgccttgca
     5341 ctcaagctgc tcgctacggt tctcgaaacg cacgcctgtt caagcgttga tttcgacaac
     5401 cgttcctgcg gggacgagat cgaacaattc gatcaggtcg gcattgccca tgcggatgca
     5461 gccatgcgag gcggggacac ccatcccctg ctgatccggc gtgccgtgga tgtagatata
     5521 gcggcgcatg gtatcgcatt cgcccagccg gttgaacccc ggctcgcacc ccgacagcca
     5581 caggatgcgc gtcagtatcc agtcgcgctg cgggaattgc gcgcccagct ccggcgtgta
     5641 gacctctccg gtggggcgcc gccccacgaa caccgcgttc tcaggctggc ccgcgccgat
     5701 cttcgcgcgc accacatgcc ggccgcgcgg cgtgcagttg ctgcccgcgc gctcgcccgc
     5761 gccgtgcgaa gccgtggaca cagaataacg gcgcagcagc tcgcccgcgt cgtcgcgcag
     5821 ttccaatgtc tgggtcggga tgtctacggt gattttcatg ctaccccgtc actcccgcgc
     5881 aggcgggagt ccagccaatt aacggttccg catcgcggac aacgccaaaa cgttgtccag
     5941 ctatgccgga tttattcccc cttctggatt cccgcctgcg cgggaatgac gaaaacattg
     6001 tcgacatgaa tttacgcact ccgttgctgc gcccggcgca tcgcgaagaa acggctgagc
     6061 gtggcactgc actcctccgc cagcaccccg cctgcgacct cggtgtgatg attcagacgc
     6121 ggctcggcga acgcatccag cacgctgccg caggccccgg tcttgagatc gcgcgcaccg
     6181 tacaccagtc gcgcgatgcg cgcatgcatg atcgccccgg cgcacatcag gcaaggctcc
     6241 agcgtcacat acagctcaca gccgaccagc cggtaattgc ccagatgctg cgccgcatcg
     6301 cgcagcgcct gcatctcggc atgggccgaa gggtcgtggc gcgagatcgg cgcgttgtag
     6361 ccgcgcccga cgatctcgcc gtccttcacc accaccgccc ccaccggcac ctcgcccgca
     6421 gcttgcgcct ggcgcgccag ttcgagcgcg gcgcgcatga acgcatcgtc gctctgctcg
     6481 gcaggagcga ttacggaaga aaagatggcc tggtcagtca tgcgcgcatt gtacgggcaa
     6541 accactgatt gccaaacgcc gggggctgtt ggagaatcgg cgcactgcag gcttccggcc
     6601 cgacgaatct caggagcgcc catgtccatc aaagaaatcg acaacctgtt gctgcgtttc
     6661 atcagcgagg tggaactgtt caggaatatc gacctggatg atgtcgccgc actgctgcgc
     6721 caatcgacgc gcatcgacgt tgcccgggac gaactggtgt tcgaagaagg cgatcagggg
     6781 tattcgatgt acatcgtcac ccgtggcgtc ttcgaggtct atcgcagcgt cgcgggccag
     6841 catgtccaca tcgcccggat cacccccggc gaacacttcg gcgagatcgc cctgctgtcc
     6901 aaccgcccgc gcagcgcctc ggtgcgcgcc accctgccca gcaccgtgat ccgtctgtcg
     6961 aagaacgccc tcctgtccga acaacgagcc gcagtacagc tgttccgcaa catggcgctg
     7021 ctgatggctc agcgcctgct gtccgtcgag gacgagatca tcctgcacaa gaccggcaga
     7081 cacccctccc agcaatagcc attcgccgcg cccccggata aagcctcgtc atctgtccag
     7141 cggactgacc acgcccctgc cgcccttgtt caacacatgg gtgtagatca tcgtcgtgga
     7201 aacatcggaa tggccgagca gctcctgcac ggtgcggatg tcgtagccgg attgcagcag
     7261 atgcgtggca aaggaatggc gcaatacatg cggggtaaca ggcttgtgaa tttctgcaat
     7321 cttcgccgct tcgcgcactg cccgttgcac actcgcctca taaagatggt ggcggcgctc
     7381 aataccggag cgcggatcgg ttgaacgcac cgcagaggga aacacaaatt gccagcccca
     7441 tgtgcgcgcg gcctttggat atttagcggc aagtgcattg ggcagataca cctcgccgaa
     7501 accggcgtcg agatcgcgct cgtgcaaagc tttcactttt accagatgcg cttgcaatgg
     7561 cagaatcaaa ttctcgggga gcacggtcac cctgtcctta ttgccttttc cttcgcgcac
     7621 gataatttcg cgccgttcaa attccacatc cttcac
//
LOCUS       DASUIV010000113         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_119946, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000113 DASUIV010000000
VERSION     DASUIV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119946"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFW59_04215"
     CDS             complement(<1..417)
                     /locus_tag="VFW59_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEX5363448.1"
                     /translation="MGWLEKIFEGTLWNSRFMVLAAVVGSLLAGIAIFYMATIDVLNL
                     FSHALHYADSSLTAEARKALHDSTVSHIVEVVDGYLLATVMMIFSLGMYELFISDIDQ
                     AHGSKASSKILVISNLDDLKSRLAKVILMIMIVTLFE"
     gene            complement(479..1690)
                     /locus_tag="VFW59_04220"
     CDS             complement(479..1690)
                     /locus_tag="VFW59_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEX5363449.1"
                     /translation="MQKNWRDFTNFMRFIVRRFNQDRCAQIAASLTFTTLLSLVPLFT
                     IALTLFSVFPVFEHYSSQIKKFLLDNLMPDMATKLITHYVQQFTESAMRLTEVGLVFL
                     AVTATFLLLTIEHAFNTIWRVQYPRPLFKRLIVYWAVLTLAPLLVGASLVLTSWIVGL
                     STGYISHVPVTGIGLLKLLPLLLTCLAFALLFYFVPNRYVPRSHALIGALVAAVAFES
                     MSRAFAYYIGHFPTYKLVYGAFASVPIFLMWIYLSWLAILFGAVIAASLSHWRMPVLH
                     LRPPSLQLLDALRLLRVLVDGLRQGKVNTLPELSKLLRLDYDALETLLDMLAQTRMVR
                     RVGANGWLMMRDAEHIRAAELWHLFVLDRASIADDRDNDPLRQWLLLCADTLERSTEI
                     SLQELFALKSD"
     gene            1719..2321
                     /gene="wrbA"
                     /locus_tag="VFW59_04225"
     CDS             1719..2321
                     /gene="wrbA"
                     /locus_tag="VFW59_04225"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX5363450.1"
                     /translation="MITDKEILVLYYSQNGATRQLAHLIARGIEQVEGARARLRTVPK
                     ISTVCEATESGVPDEGAPYVELRDLEECCGLALGSPTRFGNMAAAMKYFWDGTGGLWM
                     KHALADKPAALFTSSGSMHGGQESTLLSMMLPLLHHGMLIVGLPYSEPELGTTQSGGT
                     PYGASHVAGLTGDQAITEAEKKLCMALGRRLAQTALKLAK"
BASE COUNT          627 a          771 c          672 g          423 t
ORIGIN      
        1 ctcgaacagc gtgacgatca tgatcatcag gatcaccttg gccagacgcg acttcagatc
       61 gtccagattg ctgatgacca atatcttgct ggaggccttg ctaccatggg cctgatcgat
      121 gtcgctgatg aacagctcgt acatgcccag cgagaagatc atcatcaccg tcgccaacag
      181 ataaccatcg accacttcca cgatatgcga caccgtgctg tcatgcagcg ccttgcgcgc
      241 ttcggcagtc agactggaat cggcgtaatg cagcgcgtgg ctaaacaggt tcagcacgtc
      301 tatggtcgcc atgtagaaga tcgctatgcc tgccagcaga ctgcccacca cggccgccag
      361 caccatgaag cggctgttcc acaacgtacc ctcgaatatc ttttccaacc aacccatgtc
      421 gtacccctag taaaaaatgt gacgcattaa aacgtaaacc ggccttgcag tgcaagcatt
      481 agtcagactt caaggcaaag agttcctgca aactaatctc ggtgctgcgc tccagcgtgt
      541 cggcacagag caacaaccac tgcctcagcg gatcgttgtc ccgatcatcc gcgatcgacg
      601 cgcgatccag cacgaacaga tgccacaact cggcagcacg aatatgctcc gcatcgcgca
      661 tcatcagcca gccgttcgcc ccgactctgc gcaccatgcg agtctgcgcc agcatatcga
      721 gcagcgtctc cagcgcatcg taatcaagcc ggagcaattt cgacaattcc ggcagggtgt
      781 tgaccttgcc ttgccgcagc ccatccacca gaacgcgcaa cagccgcaac gcatcaagca
      841 attggagcga ggggggacgc aggtgcagca cgggcatacg ccaatgcgac aacgaggcag
      901 caatcaccgc cccgaacagg atcgccagcc aggacagata aatccacatc aaaaagatcg
      961 gcacgctggc gaatgcaccg tacaccagct tataggtcgg gaagtggccg atgtaataag
     1021 cgaaggcgcg gctcatcgac tcgaatgcca cagccgccac caaggccccg atcaaggcat
     1081 gcgagcgcgg cacataacgg ttggggacga aatagaacaa caaggcgaat gccaggcagg
     1141 tgagcaataa cggcagcagc ttcaacagcc cgatacctgt taccggcaca tgactgatgt
     1201 agcccgtaga caaaccgacg atccaggagg tcagcaccag actcgcaccg acaagcaagg
     1261 gcgccagcgt cagcacggcc caatagacga tcagccgctt aaacaacggg cgcggatact
     1321 gcacgcgcca gatggtatta aacgcatgct ctatcgtcag caacagaaac gtcgcggtca
     1381 cggcaagaaa caccagcccc acctccgtca gtcgcatcgc actctcggtg aattgctgca
     1441 catagtgcgt gatgagcttg gttgccatat ccggcatcag gttatcgagc agaaacttct
     1501 tgatctgcga agagtagtgc tcgaagacag gaaagacgga gaacagggtc agcgcaatcg
     1561 tgaaaagcgg caccagagac aacagcgtcg tgaaggtcaa actggctgcg atctgtgcgc
     1621 agcgatcctg attgaaacgc cgcacgataa aacgcatgaa attcgtgaag tcacgccaat
     1681 tcttttgcat ggtggagtcg tttccctata atgcgcgcat gataaccgac aaagaaatcc
     1741 tggtgctgta ctacagccaa aatggcgcta cacgccagct ggcccattta atcgcacgcg
     1801 gcatcgaaca agtggaaggc gcgcgtgcac gcctgcgcac cgtaccgaaa atctccaccg
     1861 tgtgcgaagc gaccgaatcc ggcgttccag atgaaggcgc cccctatgtc gagttacgcg
     1921 atctggaaga atgctgcggc ctggcgctgg gcagcccgac ccgcttcggc aacatggcgg
     1981 ctgcgatgaa atatttctgg gatgggacag gcggactgtg gatgaaacac gcgctggcgg
     2041 acaagcccgc cgcgctgttc acctccagcg gcagcatgca cgggggacag gaaagcaccc
     2101 tgctgtcgat gatgctgccg ctgctgcacc acggcatgct catcgtcggc ctgccttact
     2161 cagagccgga actgggcacg acacaaagcg gcggcacgcc ctacggcgcc agccatgttg
     2221 ccggactcac cggagatcag gcgatcacgg aagccgagaa gaagctctgc atggcgctgg
     2281 gccgccgtct ggcccagacc gcgctgaaac tggcgaaatg accgatcccg acacccatct
     2341 gaatcacgcc cgcgcagctc gctgcctgtt gcgagcccat cgctacggcg cgctgtccac
     2401 cctgtccaga aagtttgacg gacacccatt cgggtccatc gcaccttatc tgacagacca
     2461 cgacggcagc atactgctgt tcatttccgc gct
//
LOCUS       DASUIV010000114         9210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1328955, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000114 DASUIV010000000
VERSION     DASUIV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328955"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..566
                     /locus_tag="VFW59_04230"
     CDS             78..566
                     /locus_tag="VFW59_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX5363451.1"
                     /translation="MPSFDIVSEVEKTEVKNAVEQTNKEVGTRFDFKGSDAHVDQAEL
                     VLTVHADNEFQLDQIQDILNGKLTKRGVDIKCLEISDKIEKVSGNKVKRACTVKVGVE
                     IELAKKIVKLIKDSKMKVQASIQGDTVRITGKNRDDLQDAIAFIKKSITDFPLQYQNF
                     RD"
     gene            589..1395
                     /locus_tag="VFW59_04235"
     CDS             589..1395
                     /locus_tag="VFW59_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5363452.1"
                     /translation="MKPNTSDLLRTIRLGGIAALLAASHAACAADTPYPALVLDDVKH
                     VITAPARWERDEWRDFGLSALAVVGTAALIDRPLRDEMRRHSGTDRSVRQVERFGAEY
                     SLAVLGGFYLAGNAMDNEKSIQVAQDGLAASLIASGLVTPALKYVTGRRRPYENAGTG
                     TFRPFTNANASFPSGHTTQAFAVASVIADHYEETWIACASYSVAGLVGLARSYHDAHF
                     ASDIVAGAMIGTLVGKSVVAHNQAARSGRLALLPEVAPGRVGMRLTGQFQ"
     gene            1484..1975
                     /locus_tag="VFW59_04240"
     CDS             1484..1975
                     /locus_tag="VFW59_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363453.1"
                     /translation="MQRITMQSNPILALLFAGMLAAGPALADKPEWAGGGKPGKHEQR
                     RDREQEDDRGAQRDSRPHFGDHEREIAHGYFSEQYRRGHCPPGLAKKHNGCMPPGQAK
                     KWAMGKPLPRDVVYYDLPPSVVVQLGPPPARHRYVRIASDILLIAIGTGMVVDAIEDI
                     GRQ"
     gene            1978..2412
                     /locus_tag="VFW59_04245"
     CDS             1978..2412
                     /locus_tag="VFW59_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363454.1"
                     /translation="MKISDASPSAGMINRIDAHIEFSFKGESHELSSTLDLDRVLEKY
                     LTLPSLHLVMAVEHGIDTYSYLYEVMQEEDIRFDNAQGLAADFLVDDVFDMAGYAAQR
                     DQSQANTSLQAIALQEMGIEDLEQHPRLKNALLRAYQLGLEA"
     gene            2409..2951
                     /locus_tag="VFW59_04250"
     CDS             2409..2951
                     /locus_tag="VFW59_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HEX5363455.1"
                     /translation="MKNALVLFAEGSEELEAVTIINILRRGGVNVTLAGLHGGPLRGS
                     RGTVLLPDTTLDEALGHHYDMVVLPGGQPGTQHLKSDVRVLKLVRDMNEAGKFVTAIC
                     AAPSVLAAAGLLDGKQATCFPTCLDEFPAVRLKTSEVVEDGKLITSRGPGTAMDFALT
                     LLERLTNQAKRQEVEAGLVR"
     gene            3037..3732
                     /locus_tag="VFW59_04255"
     CDS             3037..3732
                     /locus_tag="VFW59_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5363456.1"
                     /translation="MQLPLPPLGVALLCGVAAAAFSHLAGLARWWLFIQFLFLPALVL
                     MLALNIPPGLFLAAFLILLVVYWSTFRTQVPLYLSSDKIWHALEAFLPAPIEGKGFRF
                     ADLGSGLGGVLTHLARVRTDGNFHGVESAPLPFLWSWLRIKLGGYCNCSVHWGSLWHC
                     DLGEYDVVFAYLSPVPMEQLWHKACTEMKPGSLFISSTFTIPGQSPHRSIQVDDLHRS
                     TLQVWKMGAAPQS"
     gene            complement(3743..4897)
                     /locus_tag="VFW59_04260"
     CDS             complement(3743..4897)
                     /locus_tag="VFW59_04260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5363457.1"
                     /translation="MCELTLSDLTILYAEDDEDMSGQVAHFLRKKCARVLLARNGREA
                     LELWQTQRPDMIISDVMMPEMDGLEFSEIVKQRDSAMPIILTTAFNDSAYLHRAIDIG
                     VDNYVLKPVDLSKLHAAMLKSADLLLKTRALATSRAQLEAYHHAAEEERALVSDLMKR
                     MMQPERMVDDQVQYRLNPADLVSGDLIAFYRARNDRLYLMLADSTGHGLPAALNLLPV
                     NHVFYSMTEKGLPISQIVEEMNWAVKQQSPADRFVSAFVACIDPYNHVIESWNGGIPS
                     ALMINDAGKVYHSIVPNNLPLGILDRTFVARTEIFQWNCHSQLAVYSDGLVEAEDERG
                     MAWGEETIRQVIASSPVAERFEALFNGVHEHLGARRAFDDMTLMMIDCAM"
     gene            complement(4885..8031)
                     /locus_tag="VFW59_04265"
     CDS             complement(4885..8031)
                     /locus_tag="VFW59_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5363458.1"
                     /translation="MTDTESPVTLRLLDEVFCDVSVVFYIADAAGDFTLRQVSRNIER
                     VLGLSLSACVGSPSFWRDRIHPEDIESMLREIGEMGEAKACVQEYRLRDESNDYRWVR
                     NEVALRCDESGSARYLAGCLRDISAEKARCVEFARYEEMQHAREHFYRSVLDSLPQRL
                     FWKDRNSVFRGCNRQGANALGLNGTDEIVGKSDYDFYQDREEADYLRIHDEQVMDSGQ
                     ASYHTEVSIREGDIWLDVSKVPLTDENGKVYGLLVSYENVSALKKSEMALNKFKRAVE
                     QSYNSVIITDTAGRIEYVNPMFLKAYGYEEHEVVGQMPNLLKSGLVPAEIYRELWQSI
                     LSGQHWRGELANRKRNGEVSWQSVSISPITDESGAITHFLAIEDDITEHKLLEADLKR
                     QLKFIQTLIDALPHPVYFKDCDGHYQRVNHAFEEFFGLKVGEGIGKTAFDVFPEDVAS
                     MLKVADKHLLKHGGAEMIEYTVTGRDGKLRNIMSHKAAYLNEHGEVSGIIGSHFDISE
                     RKRVEAALAENEARLRELTSTVGEGIYVVNAEQLITFANPAAVNLLGWSEQELLGRNV
                     HSLFNCPGYANTGEPDTEPCCMAELIRKLPRTVRNENESFLRKDGTPFPVSVIASPII
                     REDRYAGAVVAFHDIAEQKFTQSLLNNALQELRTVLDNAQIGVAYLRNDNFSWINRHM
                     EQMFGYTADELRQESIGVLCGTGNRKESNSIITLAMSTLHKGQAYESEFKMKRRSGEV
                     FWCHLRGVAIDPGSPASGSIWIVLDIDRLKKTERRLKVLNEGLAQQVEEETRKSLEKE
                     RLLIQQGRNAAMGEMIGNIAHQWRQPLSTLGLVVQNIHSDYREGGLDEQALDTYVDTA
                     TRAIQRMSCTIDDFRDFFRPSRVSESFSVYQSIQEAIQLLDAMLKNNGISTGLIGDTT
                     LRAFGHANEFSQVILNFMVNAKDALVEKKAAKGHIDIQLSASDERAVVTIGDNAGGID
                     EGLMERIFDPYFTTKASGTGIGLYMCKIIIEQHMNGGISCRNLFEGDKRSGVEFTITI
                     PLPSNEKDSRCEVMKCAN"
     gene            complement(8220..8480)
                     /locus_tag="VFW59_04270"
     CDS             complement(8220..8480)
                     /locus_tag="VFW59_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363459.1"
                     /translation="MSKQIVLPTWLSVLSLAVALFVPCGVGLIADASADFGNRENIVQ
                     AKGGRFVPPDEWLMVPVEPAVYQMEQSRSGEEREPAHLEHRV"
     gene            complement(8565..>9210)
                     /locus_tag="VFW59_04275"
     CDS             complement(8565..>9210)
                     /locus_tag="VFW59_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5363460.1"
                     /translation="IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLA
                     QRSAAAAKEIKTLIGDSVEKVEGGSKLVAQAGQTMEEIVTSIKRVTDIMSEITAASSE
                     QSQGIEQVNTAITQMDEVTQQNAALVEEAAAAAESLEEQAQNLSVAVATFKVDTSTAV
                     SRPSAPASHPAPVRKPAAHPAAKSSAKAGKAAKPAALTAKPESQGGEDGEWSEF"
BASE COUNT         1969 a         2935 c         2447 g         1859 t
ORIGIN      
        1 gaaacgaacc tgacagtccc cgtccgggca caagagtcaa cgcaacgagt tgcggttaaa
       61 caaacaagga gatactcatg ccatcattcg acatcgtttc cgaagtagag aagaccgaag
      121 tcaagaacgc ggtcgagcag accaacaagg aagtcggcac gcgcttcgac ttcaagggca
      181 gcgacgccca tgtcgaccag gccgaactgg tactcaccgt ccatgccgat aacgaatttc
      241 agctcgatca gatacaggac atcctgaacg gcaagctgac caagcgcggc gtagacatca
      301 agtgtctgga gatcagcgac aagatcgaga aggtcagcgg caacaaggtg aagcgcgcct
      361 gcaccgtcaa ggtcggcgtg gagatcgagc tggcgaagaa gatcgtcaag ctgatcaagg
      421 acagcaagat gaaagtgcag gccagcattc agggcgacac cgtgcgcatc accggcaaga
      481 accgcgacga cctgcaagac gccatcgcct tcatcaagaa gtccatcacc gacttcccgc
      541 tgcagtacca gaacttccgc gactaagcct ctcactggag cgcatcccat gaaaccgaat
      601 acctcggatc ttctgcgcac catccgactc ggcggtatcg cggcgctgct ggcggcgagc
      661 catgcagcct gcgccgctga cactccctac cctgcactgg tactcgacga tgtaaagcac
      721 gtcatcacgg ctccggcacg ctgggaacgc gatgagtgga gggacttcgg gctgagcgca
      781 ctcgctgtgg tcggcaccgc cgcactgatc gaccgaccgc tgcgcgacga gatgcgccgc
      841 catagcggca ccgaccgctc cgtgcgccag gtcgaacgct tcggtgcgga atactctctg
      901 gcggtgctgg gcggcttcta ccttgccggc aatgcgatgg ataacgagaa gagcatacag
      961 gtcgcacagg atggattggc cgccagcctc atcgccagcg ggcttgtcac ccccgcactc
     1021 aaatacgtca ccgggcgccg ccgcccctac gagaacgcgg gaactggcac cttcaggcca
     1081 ttcaccaacg ccaacgcctc cttcccttcc ggtcacacga cgcaagcctt cgccgtggct
     1141 tcggtgatcg ccgaccatta tgaagaaacc tggatcgcct gcgcctccta ctctgttgcc
     1201 gggctggtcg gtctcgcacg cagctaccac gatgcgcatt tcgcatcgga catcgtggcg
     1261 ggcgcgatga taggcactct ggtcggcaag agcgtcgttg cgcacaatca ggcggcgcgc
     1321 tccggcaggc tggcgctgct gccagaggtt gcgccgggca gggtcgggat gagactgact
     1381 ggccagttcc agtaggccta ccacatcagg aattccccat tgcgggtgaa atccgtctgg
     1441 gtaacaatgc ccctcccgca acgctcattt ctggaggtcg atcatgcaac gaatcacgat
     1501 gcaatccaat cccatactgg ctcttctgtt cgcgggcatg ctcgcggccg ggccagcgct
     1561 cgccgacaag ccggaatggg ctggcggcgg aaaacccgga aaacatgaac aaaggcgcga
     1621 tcgggagcag gaagatgaca gaggcgcgca gcgcgacagt cgcccgcatt tcggcgacca
     1681 tgagcgggaa atcgcccacg gctatttttc cgaacaatac cgcagaggac actgtccgcc
     1741 gggacttgcc aagaagcaca acggctgcat gccgcccgga caggccaaga agtgggcgat
     1801 gggcaaaccg ctgccgcgag acgtcgttta ttatgaccta ccacccagcg tcgtggtgca
     1861 gctcggccct ccgccggcta gacaccgcta cgtacgcatc gcgagcgaca tactgctgat
     1921 tgcgatcggg acgggcatgg tcgtggatgc aatcgaagat atagggcgcc agtaaacatg
     1981 aaaatatccg atgcatcacc gtcggcaggc atgatcaacc gcatcgacgc acacatcgaa
     2041 ttcagcttca agggtgagag ccatgaactc tcatccacgc tggatctgga tcgggtgctg
     2101 gaaaagtatc tcaccctgcc gtcgctacat ctcgtgatgg ccgtggaaca cggcatcgac
     2161 acctactcct acctctatga ggtgatgcag gaagaagaca ttcgtttcga caatgcacag
     2221 ggactggccg cagactttct ggtggatgac gtgttcgaca tggcgggcta cgccgcacag
     2281 cgggatcaaa gtcaggcgaa cacctcgctg caagccatcg cgctgcagga gatgggcatc
     2341 gaagatctcg aacaacatcc gcgcctgaag aatgcgctgc tcagagccta tcaactggga
     2401 ctggaagcat gaaaaacgca ctggtactgt tcgccgaggg cagcgaggaa ctcgaagccg
     2461 tcaccatcat caacatcctg cggcgcggcg gtgtcaacgt cactctggcg ggcctgcatg
     2521 gcggcccatt gcgcggcagt cgcggtacgg tgctgctgcc ggacaccacg ctggatgaag
     2581 cgctcggcca tcactacgac atggtcgtcc tgcctggcgg ccaacccggc acccagcatc
     2641 tcaaatccga tgtccgcgtg ctgaaactgg tacgagacat gaacgaggca ggaaaatttg
     2701 tcactgcgat ctgcgccgca ccctccgtgc tggccgccgc aggcctgctc gatggaaagc
     2761 aggccacctg cttcccgaca tgcctcgacg aattcccggc ggtcaggctg aaaacctccg
     2821 aggtcgtgga ggacggcaaa ctcatcacct cgcgcggccc gggcacggcc atggatttcg
     2881 cactgacact gctggaacgc ctgaccaatc aggctaaacg gcaggaagtc gaggcaggac
     2941 tggtgcgctg atctgttctc ccgactcccc cccgccgtca ccgccctgct gcttcaatgc
     3001 ctggcgttcc tgctgacagt cctgagcgta cgcgctgtgc agctgccgct tccaccgctg
     3061 ggtgtcgcgc tgctgtgcgg cgtagcggcc gccgccttca gccatctagc cggactggcg
     3121 cgctggtggc tgttcatcca gtttctgttc ctgcccgcgc tggtgttgat gctggcgctg
     3181 aacatccccc ccggcctctt ccttgcagcc ttcctcatcc tgctggtcgt gtactggagc
     3241 accttccgca ctcaggttcc gctgtatcta tccagcgata aaatctggca cgcactggag
     3301 gcgtttttgc ccgcgcccat tgaaggaaag ggattccgct tcgctgacct cggctccggt
     3361 ctgggtggtg tgctgacgca cctcgctcgc gttcgcacgg acggcaattt ccacggcgtg
     3421 gaatctgcgc cgctgccttt tctgtggagt tggctgcgca tcaagctcgg cggatactgc
     3481 aattgcagcg tgcactgggg tagcctgtgg cattgcgatc tcggcgaata cgacgtggtg
     3541 ttcgcctacc tctcgccagt gccgatggaa cagctatggc acaaggcctg caccgagatg
     3601 aagccgggca gcctgttcat cagcagcacc tttaccatac cgggacagtc gccacatcgc
     3661 agcatacagg tggatgatct ccatcgttcc accttgcagg tctggaagat gggagcggca
     3721 ccgcagagct aggacagcgc catcacatgg cgcaatcgat catcatcagc gtcatatcgt
     3781 caaatgcgcg tcgcgcgcca agatgttcgt gcaccccgtt gaacaaagcc tcaaaacgct
     3841 ccgccaccgg gctgctggcg atcacctgcc gaatggtctc ttcgccccac gccatgcccc
     3901 gctcatcctc ggcttcaacc agaccatcgg aatagactgc cagctggcta tggcaattcc
     3961 actgaaatat ctcggtgcgc gcgacgaagg tacgatccag tatgcccaga ggcaggttgt
     4021 tgggaacgat gctgtgatag accttgcccg catcgttgat catcaacgcc gagggaatgc
     4081 cgccgttcca gctttcaatg acgtgattat agggatcgat gcacgcgacg aatgccgaga
     4141 cgaaccgatc cgctggcgac tgttgcttca ccgcccagtt catctcctca acgatctgtg
     4201 aaatcggcaa tcctttctcc gtcatgctgt agaacacatg attcaccggc aacagattga
     4261 gtgcggccgg caggccgtgg ccggttgagt cggccagcat gagatacaaa cggtcattac
     4321 gcgcgcgata aaaagcgatc agatcgccgc taaccagatc agccgggttg agccggtact
     4381 gcacctgatc gtccaccatg cgctccggct gcatcatgcg cttcatcaag tccgagacca
     4441 gcgcgcgctc ctcctccgcc gcatggtggt aggcctcaag ttgcgcgcga ctggtcgcca
     4501 gagcgcgggt cttgagcagc agatccgcgc tcttcaacat cgcggcatgc agcttgctca
     4561 gatcgaccgg tttaagcacg taattatcga cgccgatatc gatggctcgg tgcagatagg
     4621 ccgaatcgtt gaaggccgta gtcagaatga tgggcatggc agaatcgcgt tgcttgacga
     4681 tctcggaaaa ttccagcccg tccatttccg gcatcatcac atcgctgatg atcatgtcgg
     4741 ggcgctgcgt ctgccacagc tcaagcgcct cgcgcccatt gcgcgccagc aaaacgcgtg
     4801 cgcacttctt gcgcaaaaaa tgcgccacct gcccgctcat atcctcatcg tcctcggcat
     4861 agagaatggt caggtcactc agtgttagtt cgcacatttc atcacctcac aacggctatc
     4921 cttttcgttc gatggcaacg ggatggtgat ggtgaactcg accccgctcc gcttatctcc
     4981 ctcaaacaga ttgcggcaac tgataccacc attcatatgc tgctcgatga tgattttgca
     5041 catgtacaaa ccgatgccag tcccgctcgc cttggttgta aagtaggggt cgaagattct
     5101 ctccatcaaa ccctcgtcga tccccccggc gttgtcgccg atcgtcacca cagcacgctc
     5161 atcgctagca gagagctgga tgtcgatatg tcccttggct gccttttttt ccaccaatgc
     5221 atccttggcg ttgaccataa aattcagaat cacctgagag aattcattgg catggccaaa
     5281 ggccctcagc gtggtatcgc ctatcaggcc ggttgaaatg ccattgttct tcagcatcgc
     5341 atcaagcagc tgaatggctt cctgaatgga ttgatagaca gagaaactct cagaaacccg
     5401 gctcggcctg aagaaatccc gaaaatcatc gatggtgcac gacatgcgct gaatcgcacg
     5461 cgtagccgta tcgacatagg tatccagcgc ctgctcgtcc agccccccct cgcgatagtc
     5521 agaatgaatg ttctgcacca ccagccccag ggtactcaat ggctggcgcc actgatgcgc
     5581 gatatttccg atcatctcac ccatcgccgc attgcgcccc tgctgaatca gcaaacgttc
     5641 cttctcaagc gacttccggg tttcctcttc cacttgctgc gccagcccct cgttcagcac
     5701 cttgaggcga cgttccgttt ttttcagccg atcgatatcc agcacgatcc agatactgcc
     5761 gctggcggga cttcccggat cgatcgccac accacgcaaa tgacaccaga acacctcgcc
     5821 ggaacggcgc ttcatcttga attcgctctc ataggcttgc cccttgtgca acgtcgacat
     5881 tgccaaggtg atgatcgaat tactctcttt gcgattgccc gtgccgcaca aaacaccgat
     5941 cgattcctgc cgcaactcgt cggcggtata gccaaacatc tgctccatgt ggcggttgat
     6001 ccaggagaaa ttatcattgc gcaaataagc tacgccgatc tgcgcgttgt ccagcacggt
     6061 acgcaactcc tgcaaggcat tgttgagcag gctctgggtg aacttctgct cggcgatatc
     6121 atggaacgcg acaaccgccc cggcatagcg atcctcgcga atgatcggcg aagcgatcac
     6181 cgacaccggg aacggcgtac cgtctttgcg caagaacgac tcattctcat tccgcacggt
     6241 gcgcggcaac ttccggatca attccgccat gcagcagggc tccgtatccg gctcgcctgt
     6301 attcgcatat ccggggcaat taaagagact atgcacgttg cgccccagca actcttgttc
     6361 gctccagccg agcaagttga ccgcagccgg attggcgaag gtgatgagct gctctgcatt
     6421 cacgacataa atcccctcgc caaccgtgct ggtcagttcg cgcagacgcg cttcattctc
     6481 cgccagcgcc gcctccaccc gtttacgctc ggagatatcg aaatgactgc cgataatgcc
     6541 cgacacttcg ccatgctcgt tcagatatgc cgccttgtgc gacatgatat tgcgcaattt
     6601 tccatctctg ccggtcaccg tatattcaat catctccgcg ccaccatgct tgagcaggtg
     6661 cttgtcggcg actttcagca tactggcgac atcctcggga aaaacgtcga aagccgtctt
     6721 gccaatcccc tcgcccacct tcaaaccgaa gaattcctcg aatgcatgat tgacccgctg
     6781 atagtgtccg tcgcaatcct tgaagtagac cgggtgaggc agcgcatcga tcaacgtctg
     6841 aataaatttc agctggcgtt tgaggtccgc ctcaagcaac ttgtgctcgg tgatgtcatc
     6901 ttcaatggcc agaaaatgag tgatggcacc tgattcgtcg gtaatcggcg agatggatac
     6961 ggattgccag ctcacctctc cgttacgctt tcgattagcc aattcaccgc gccaatgctg
     7021 gccgctcaga atggattgcc acagctcacg atagatctcg gctggtacca gacccgactt
     7081 cagcaaattc ggcatctgcc ctaccacctc gtgttcctca tagccgtagg ccttcaggaa
     7141 catcggattg acatactcga tacgccctgc cgtatcggtg atgataacgc tgttgtaact
     7201 ctgctctacc gcgcgtttga acttgttcaa tgccatctcg ctcttcttga gcgcactcac
     7261 attttcgtaa ctcaccagca agccgtatac cttgccattc tcgtcggtca aagggacttt
     7321 gctcacgtcc agccatatat caccctcacg gatggagacc tcggtgtggt aggacgcctg
     7381 accggaatcc attacctgct catcgtggat gcgcaggtag tcggcctctt cccgatcctg
     7441 ataaaagtcg tagtcgctct tgccgacgat ttcatccgta ccgttcaggc caagcgcatt
     7501 cgccccctga cggttacaac ccctgaaaac agagtttctg tctttccaga acagacgctg
     7561 cggtagactg tccagcacgg agcggtaaaa atgctcccgt gcatgctgca tttcctcata
     7621 acgggcgaac tcgacacaac gagccttctc cgcactgatg tcgcgcaggc atccggccag
     7681 atagcgcgcc gagcctgact catcgcaccg caatgcgact tcattgcgaa cccaacggta
     7741 atcattcgac tcgtcgcgca aacggtattc ctgcacgcag gccttcgctt cgcccatctc
     7801 tccgatctcg cgcagcatgc tctcgatatc ctccgggtgg atacggtcac gccagaagga
     7861 tggagaaccc acacaagcac tcagcgacaa gcccaacaca cgttcgatat tgcggctcac
     7921 ctgacgtagc gtgaaatccc ccgccgcatc agcgatgtag aacacgacag aaacatcgca
     7981 gaacacctcg tccagcagcc tcaacgtgac tgggctctct gtatctgtca cagacatctc
     8041 ccgcctttca atcaacaact ggtcaggatt ccccatgctc aggactcctc aaaaaactcg
     8101 gcttcatcgc tcccgaaaga acgagcgcgg cgaagtgtcg agcttgcgac caagcggcat
     8161 cccggttcaa tcaacaaacc gaatcaccac cggcgcagaa gtgaccgcta ttgagacaac
     8221 tacacccgat gctccaaatg agcaggctcc cgctcttccc ctgagcggct ttgctccatt
     8281 tgatacacag caggttcgac cggcaccatg agccactcat ccggcggcac gaagcgccca
     8341 ccctttgcct gtacgatatt ttcccgatta ccgaaatccg ctgaagcatc ggcaatcaac
     8401 cccaccccgc aaggaacaaa caaggcgacc gccaaagaca gtacgctcag ccatgtcggc
     8461 aaaacaattt gcttactcat tgcaacctcc tgtcaaaacc agcatccctc gctacggacg
     8521 ggtcaatgcc ccgttgtgcg ggctggggca gcgcccctgc cctcctagaa ctcagaccac
     8581 tcaccatctt ctccgccttg tgactcgggc ttcgccgtca acgccgcagg cttggcggcc
     8641 ttacccgcct tggccgatga cttggcggcg ggatgggctg cgggcttgcg gacaggcgca
     8701 gggtgagagg cgggggcaga tggccgcgac accgcagtgc tggtatccac cttgaaggtc
     8761 gccaccgcca ccgacagatt ctgtgcctgc tcttcgagcg attccgccgc cgcagccgct
     8821 tcctcgacca gcgccgcgtt ctgctgcgtc acttcgtcca tctgggtgat cgcggtgttc
     8881 acctgctcga taccctgact ctgctcactg gatgccgcag tgatctcgga catgatgtcg
     8941 gtcacgcgct tgatcgaggt gacgatctct tccatggtct gaccggcctg cgccaccagc
     9001 ttgctgccac cttcgacctt ctcgaccgaa tcgccgatca gcgtcttgat ctctttcgcc
     9061 gcagccgcag agcgttgtgc caggttgcgc acttcgcccg cgaccaccgc gaaaccgcgg
     9121 ccctgctcgc ccgcacgggc ggcttccact gcagcgttca gcgcgaggat gttggtctgg
     9181 aacgcgatgc cgtcgatcac gctgatgatg
//
LOCUS       DASUIV010000115         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_492846, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000115 DASUIV010000000
VERSION     DASUIV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492846"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFW59_04280"
     CDS             <1..279
                     /locus_tag="VFW59_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX5363461.1"
                     /translation="VPDVFHLSIQKGLDIAIEDVHAPNIADKIPLWYKENIDAPCFML
                     LPIMVKEKVIGLFYADMIEANALKLSQQQLSLLRTLRNQAVLAIKQKT"
     gene            389..838
                     /locus_tag="VFW59_04285"
     CDS             389..838
                     /locus_tag="VFW59_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5363462.1"
                     /translation="MNLVKWDPFKELEDMSSRLNRLFGRSMLPVESDRELLSVADWMP
                     SVDISETESAYLIKAEIPGVQKEDVKVTIEDGMLTLQGERKQEKEEKDKKFHRIERSY
                     GCFMRSFRVPDDADESAIKAEFKDGLLNVTLNKSAKSRANAVNVAIS"
     gene            884..1384
                     /locus_tag="VFW59_04290"
     CDS             884..1384
                     /locus_tag="VFW59_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEX5363463.1"
                     /translation="MGHTTHPPGFHLVRRDRLQQEMLQDSYKSKAKLPEPTVCPTCGA
                     VFHGGGWKWLEKPKEAHQETCPACHRIHDRFPAGYLSLSGEFLAAHEAEIMHLVKHHE
                     VREKAEHPLQRIMDIEMSEQDCQITTTDTHLARGIGEALHHAYQGELEYHYNPDQNLL
                     RVSWSR"
     gene            complement(1381..1842)
                     /locus_tag="VFW59_04295"
     CDS             complement(1381..1842)
                     /locus_tag="VFW59_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5363464.1"
                     /translation="MYQRILVPIDGSETSDRALHEAIKLADGKAQLHLVHVVEEVYPL
                     DAEGYAFIDYAALQEAARHSGERLLAKAKSAAKQAGATPDAVLLETAGERVASVIDRE
                     AVKWSADLIVLGTHGRSGFTRLLFGSVAEGVVRSATVPVLLVRSAEQQSPA"
     gene            complement(1874..2419)
                     /gene="orn"
                     /locus_tag="VFW59_04300"
     CDS             complement(1874..2419)
                     /gene="orn"
                     /locus_tag="VFW59_04300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX5363465.1"
                     /translation="MAQNQNHLIWLDMEMTGLLPDSDRIIEIAMVITDSNLETVAEAP
                     VLVVHQPASVMDGMDAWNQSTHGKSGLIDKVKASTLSEAMVEEQMLEFLKEYVPIRTS
                     PMCGNSICQDRRFMARYMPKLEDYFHYRNLDVSTLKELAKRWNPAVAQGLKKHGKHEA
                     LADIYESIAEMRHYRDNFLKL"
     gene            2498..3751
                     /locus_tag="VFW59_04305"
     CDS             2498..3751
                     /locus_tag="VFW59_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5363466.1"
                     /translation="MTATQFTLLFLAALALTTLTRLWLARRHLSHIAAHRAAVPETFS
                     ARVSLTEHQKAADYTSAKTRFGMIGTLFDGAMLLAFTVAGGIQFIAERCNGWFDSPLM
                     QGVATIVAVLMLSSLLETPFSLYRTFVIEERFGFNKMTLALYFKDALKGLLLGAALGL
                     PLLFGVLWLMSAMGEYWWLYVWLVWMIFNLLVLFIYPTYIAPLFNKFTPLQDEAMKAR
                     IEALLARCGFTASGLFVMDGSRRSAHGNAYFTGFGKSKRIVFFDTLLERLDGGEIEAV
                     LAHELGHFKRRHVIKRIAMTFAISLGFLWLLGQLMQTEWFYAGLGVLPATTPSTALAL
                     LLFFLTLPVFSFLLNPLLSAYSRRHEFEADAYAAQQTGSGNLIGALVKLYQDNAATLT
                     PDPLYSKFYDSHPPAMVRIAHLQKL"
     gene            3761..4066
                     /locus_tag="VFW59_04310"
     CDS             3761..4066
                     /locus_tag="VFW59_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5363467.1"
                     /translation="MRLITAFALLCSATAAVANPFPNGDAQTGQKLFDQYKCARCHAS
                     MLGGDGSAIFTRADRKVHNAAQLVEQMGMCTGNLGITLTPQDEQHLGAYLNRYYKLK"
BASE COUNT          876 a         1354 c         1135 g          795 t
ORIGIN      
        1 gtgccggacg tattccatct gtctatccag aaaggtctgg acatcgccat cgaggatgtc
       61 cacgcgccca atatcgccga caagattccg ctctggtaca aggaaaacat cgacgccccc
      121 tgcttcatgc tcttgcccat catggtcaaa gagaaggtga tcggactgtt ctatgccgac
      181 atgatagaag cgaacgcgct caaactctcg cagcagcagc tttccttgct gcgcacgctg
      241 cgaaatcagg ccgtgctggc catcaaacag aagacctgaa cgcaagtcgc tcgcattttc
      301 ctgatagcga aatcatcctt ctccgatagc gccgcgacaa agcggcgtga caccatcgag
      361 ctcccttaac aagcaaagga gagtcatcgt gaatctggtc aaatgggatc cattcaaaga
      421 gctggaagat atgtccagcc ggctgaaccg gctgttcgga cgctcgatgc tgccggtcga
      481 gtcggatcgc gagctgctgt cggtagcaga ctggatgcca tcggtcgaca tcagcgaaac
      541 cgagagcgcc tacctgatca aggccgaaat tcccggcgtc cagaaggaag acgtcaaagt
      601 caccatcgag gatggcatgc tcaccctgca gggggagcgc aagcaggaaa aagaagagaa
      661 agacaagaag ttccaccgca tcgaacgctc ctacggctgc ttcatgcgca gcttccgcgt
      721 gcccgacgat gccgacgaat cggcaatcaa ggcagaattc aaggacggcc tgctcaacgt
      781 cacactcaac aagtcagcga agagccgcgc caacgcagtc aacgtcgcaa tctcgtaaac
      841 ccgcgccgcg cctgcgggag cggccaccta tcaggagaca atcatgggtc acactactca
      901 ccctcccggc ttccatctgg ttcgccgcga ccggctgcaa caggagatgc tgcaggacag
      961 ttacaagagc aaggccaaac tgcccgagcc gacggtctgc ccgacctgcg gtgcggtctt
     1021 ccacggcggc ggatggaaat ggctggaaaa accgaaagag gcccatcagg aaacctgccc
     1081 tgcctgccat cgcatccatg accgattccc ggccggctat ctctcgttaa gcggcgaatt
     1141 cctcgcggca cacgaggcgg agatcatgca tctggtcaaa catcatgagg tgcgagagaa
     1201 agccgagcat cctttgcaac gcatcatgga catcgaaatg agcgaacagg attgccagat
     1261 caccaccacc gacacgcatc tggcacgcgg catcggcgag gcattgcacc acgcctatca
     1321 gggcgaactg gaataccact acaacccgga tcagaacctg ctgcgcgtca gctggagccg
     1381 ctaagccggt gactgctgct cggcgctacg caccagcaac accggtaccg tggcgctgcg
     1441 caccacgcct tcggccacgc tgccgaacaa cagccgggta aaacccgaac gtccgtgcgt
     1501 gccgagcacg atcaggtcgg cggaccattt cacggcctcc ctgtcgatca cgctggcgac
     1561 gcgctcgcct gcggtctcaa gcaacaccgc atccggcgtc gcgccggcct gctttgccgc
     1621 actcttggcc ttggccagca aacgctcgcc gctgtgccgc gccgcctcct gcaacgcggc
     1681 atagtcgatg aaggcatagc cctcggcatc cagcggatag acctcctcga ccacatgcac
     1741 cagatgcagc tgcgccttgc cgtccgccag cttgattgcc tcgtgcaagg cacgatcgga
     1801 agtttcgcta ccgtcgattg gaaccagaat acgttggtac atgatcagtc ctccaatgcg
     1861 gcacgctggc cgtttacagt ttcaggaaat tgtcgcggta atgacgcatc tcggcgatgg
     1921 actcgtaaat atcggccagc gcctcatgct tgccatgttt cttaagcccc tgtgccaccg
     1981 caggattcca gcgcttggcc agttccttga gggtgctcac gtccagattg cgatagtgga
     2041 aatagtcttc cagcttgggc atgtagcgcg ccatgaagcg cctgtcctga cagatggagt
     2101 tgccgcacat cggcgaagta cggatcggca catattcctt gaggaattcc agcatctgct
     2161 cctccaccat cgcctcggaa agcgtcgagg ccttgacctt gtcgatcagc ccggacttgc
     2221 cgtgcgtgga ttgattccag gcatccatgc cgtccatcac gctggccggt tgatgcacca
     2281 ccagcactgg cgcctcggcg acggtttcga gattggagtc ggtgattacc atcgcgattt
     2341 cgatgatacg gtcgctgtcc ggcaatagcc cggtcatttc catgtctagc cagataaggt
     2401 ggttttggtt ttgtgccata attcgcgcgt ctaaacaatt gaacagtaag gtgcgtattg
     2461 tgtcacaacc gtaccctcaa cgaacaccca cccccacatg accgcaacgc aattcaccct
     2521 gctcttcctc gctgcccttg ccctgaccac gctgacccgc ttgtggctgg cgcgccgcca
     2581 tctgtcccat atcgccgcgc atcgcgccgc cgtgccggaa acattcagcg ccagggtatc
     2641 gctgaccgaa caccagaaag cggccgacta cacctcggcc aagacccgct tcggcatgat
     2701 aggcacgctg ttcgatggcg ccatgttgct ggccttcacc gtcgcaggcg gcatccagtt
     2761 catcgccgag cgctgcaacg gctggttcga cagcccgctc atgcaaggcg tggcgaccat
     2821 cgtcgcggtg ctgatgctgt cgtcgctgct ggaaacgccg ttcagcctgt accgcacctt
     2881 cgtgatcgag gagcgcttcg gtttcaacaa gatgaccctc gcgctgtatt tcaaggatgc
     2941 attgaaaggc ctgctgctcg gcgccgcact cggcctgccg ctgctgttcg gcgtgctctg
     3001 gctgatgtcc gcgatgggcg agtactggtg gctgtatgtg tggctggtat ggatgatatt
     3061 caacctgctg gtgctgttca tctaccccac ttatatcgcc ccgctgttca acaaattcac
     3121 ccccttgcag gacgaggcga tgaaggcgcg tatcgaggcg ctgctggccc gctgcggctt
     3181 caccgccagc ggcctgttcg tgatggacgg cagccgccgc agcgcgcacg gcaacgccta
     3241 cttcaccggc ttcggcaaga gcaaacgcat cgtgttcttc gacacgctgc tggagcgcct
     3301 cgacggcggc gagatcgagg cggtgctggc gcacgaactc ggccacttca aacgccgcca
     3361 tgtgatcaag cgcatcgcca tgaccttcgc catcagcctc ggattcctgt ggctgctcgg
     3421 acaactgatg cagaccgagt ggttctacgc aggactgggc gtcctccccg cgacaacgcc
     3481 atccaccgcg ctcgcgctgc tgctgttttt cctgaccctg ccggtattca gcttcctgct
     3541 gaatcccctg ctgtcggcct attcgcgccg ccatgaattc gaggccgatg cctacgcggc
     3601 acagcagacc ggctcgggca atctgatcgg cgcattggta aaactgtatc aggacaacgc
     3661 ggcgacactc acgcccgacc cgctgtattc caagttctac gactctcacc cgcccgcgat
     3721 ggtacgcatc gcccatttgc aaaaactgta ggaggatcac atgagactca tcacggcctt
     3781 cgccctgctc tgctccgcaa ccgcagccgt cgccaatccc ttcccgaacg gcgatgcgca
     3841 gaccggacag aagctgttcg atcaatacaa atgcgcccgc tgccatgcct ccatgctggg
     3901 cggagacggc agcgcgatat tcacccgcgc cgaccgcaag gtgcataacg cggcgcaact
     3961 ggtcgagcag atgggcatgt gcaccggcaa tctcggcatc accctgacac cgcaggacga
     4021 gcagcatctc ggcgcctacc tcaaccgcta ttacaagcta aagtagtccc catgaataac
     4081 tcatcagcag cgtcaacgcg cgcgccgttt ctgtcttgag cacctgtcct gagcccgctc
     4141 gagtgaccac cctgcacgga
//
LOCUS       DASUIV010000116        18797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1143869, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000116 DASUIV010000000
VERSION     DASUIV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143869"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1924)
                     /locus_tag="VFW59_04315"
     CDS             complement(221..1924)
                     /locus_tag="VFW59_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5363468.1"
                     /translation="MMELTDSLLESFQKKAHIPPVPLAGMISAANNDAIDFAQFVIQD
                     GYLDRDTAGSLLASQINRTYVNLSKTLFQDEVVSLLTGEIAQHYQAIPIYQLGQVVTM
                     GMVTPNDAKIVKELGNLLMKSISPVFCFRDEVNSAIKVTYQSAQHVDDLVMSFDLSVF
                     QSTELSNAALAKLLDSKPLIELGDSIILLALKERASDIHIEPKRNEVVVRFRIDGDMV
                     DRLFLPAGMSLPLTSRYKITSGMDITERRRPQDGRFSFSLPMKVLDLRVSCLPVMHGE
                     KVVIRILGSLYSSDILNLDKLDFSPEVLRPFKATLKRPQGILFVTGPTGSGKSTTLYA
                     ALNFINTRTTNIITIEDPIEYEVRDLNQVMVNDRVGRSFKDVLRSVLRQDPDAILVGE
                     VRDTETARIAAQAALTGHLVLTTLHTNDAIQATTRLLEMGVENFIVAPSIIGVLGQRL
                     VKRLCEFCKAEHRHDPDELRQYFFWLEGAELPVFYKGKGCKRCNGTGYTGRVGIHEFL
                     KISPQIREAILHGRSYDEILAIATQQGFRNLRYDGFKKALRGLTSLEEVIRATSSLED
                     D"
     gene            complement(1924..2238)
                     /locus_tag="VFW59_04320"
     CDS             complement(1924..2238)
                     /locus_tag="VFW59_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5363469.1"
                     /translation="MKCNHTNPAGYVFCGTCGAALEYARCRCGFVTGVGELFCGQCGI
                     NLIEQAAGSGRAGVFVDQRFDLDHLAQQAAQEKQFLGSTDKARVTQDDIRKLLTTRRK
                     RF"
     gene            complement(2241..2651)
                     /locus_tag="VFW59_04325"
     CDS             complement(2241..2651)
                     /locus_tag="VFW59_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5363470.1"
                     /translation="METRKVRILIADDERHIRSLISLIVTSLGAEVVAEAEDGEQALS
                     LYRQVSPDMVLLDINMPKMDGVAVLKQIMAINKKTLVVILTSLNTLELVKECIDNGAW
                     NYILKNTTANELHKEISTTWGEYMAALKAVGLDV"
     gene            complement(2782..3372)
                     /locus_tag="VFW59_04330"
     CDS             complement(2782..3372)
                     /locus_tag="VFW59_04330"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX5363471.1"
                     /translation="MLRAELFSIEQLKRHAVVLAGQHQIDPSQGPDKFLPRLAGNERV
                     TTRRRDMVWFMPAVLALGLLPSPVFAESNCPGIHVKILNIKNSTGTVACALFDASAGF
                     PTEYLRMATNVMVIKIRKDQARCDFEDIPQGNYAMAVVHDENMNGKLDTNWLGVPMEG
                     YGFSNDAKGTLGAPSFSAASFPYNGQNLELTMTLNY"
     gene            3808..4191
                     /locus_tag="VFW59_04335"
     CDS             3808..4191
                     /locus_tag="VFW59_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEX5363472.1"
                     /translation="MAKSMLQLAIPESGFRFSIEESDMLVTFRTNAYPNISMFGDDAL
                     AMLKMMGHSATVPGSIRAEDVAYALSRLNDAVGKQKSPPPVAVEKGEEPAVSLAHRAM
                     PLIDLLAAAVKANDYVMWDKSTIIG"
     gene            4203..4385
                     /locus_tag="VFW59_04340"
     CDS             4203..4385
                     /locus_tag="VFW59_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363473.1"
                     /translation="MKKMEQLELDAYRDEIGADMRGLLEKYRAIFGWDIPEIDQQGAD
                     KLILAAMHKALDDIAV"
     gene            complement(4468..4848)
                     /locus_tag="VFW59_04345"
     CDS             complement(4468..4848)
                     /locus_tag="VFW59_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5363474.1"
                     /translation="MTNKTKGSDSERKVLEHAHRGIQMLTEYKPKLMGKVQAFMAEAM
                     QPGVLDKKEKERIALGMALTQQCHYCIALHVRACKHAGVTLEEIMEVCAIGVMMGGGP
                     VLTHMAEVERALNEFYLDENGDPV"
     gene            complement(4876..5895)
                     /locus_tag="VFW59_04350"
     CDS             complement(4876..5895)
                     /locus_tag="VFW59_04350"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX5363475.1"
                     /translation="MYSLLRPALFRLDPETAHHLTLDGLNAAQALGLNGLMARRPADD
                     PRTVMGLTFPNPVGLAAGLDKNGDCINGLAALGFGFVEIGTVTPLPQPGNPKPRLFRL
                     PEAQGIINRMGFNNDGVDALIANVQRAGFKGVLGINIGKNAATPIEKAADDYLICLRK
                     VYAHASYVTVNISSPNTKNLRQLQGGDELDALLSQLKAEQEKLAQQHGRHVPVAVKIA
                     PDLDADQIRNIAALLMRHRIDGVIATNTTLSRDGVETLPHGNETGGLSGAPVRDKSTA
                     VIRSLAAELQGTLPIIGAGGILSGADAVEKMRAGAALVQVYSGLIYRGPALVAECANA
                     IRAMH"
     gene            complement(5899..6771)
                     /locus_tag="VFW59_04355"
     CDS             complement(5899..6771)
                     /locus_tag="VFW59_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363476.1"
                     /translation="MKTRLFIFIAALLLLPPAGFWLGGHEWGELAAQETRAPHDPVAL
                     TLLVLLGYTLLTGLLVKLRTGNNPLVMQRGYYLAMSATGAVTGWLLVYLSRFGTDWAT
                     TSGGLLPMLCDTLLFALLAPAVLGTRALIGSFPRLLKALARGLPLPSPANETAALTLT
                     AIAAFGLLGGAALPAQQYWLLWTAPLLLLFALQLLWHEGTLFHGLKNGNWGRPVCAAL
                     AGLIVGNLAILIYQAAGGVPKPQLLAQSGYILFGLLSLQLGDVVAEHWRGTQRHAPIK
                     PKKKFPIPVVVKKS"
     gene            complement(6768..7559)
                     /locus_tag="VFW59_04360"
     CDS             complement(6768..7559)
                     /locus_tag="VFW59_04360"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX5363477.1"
                     /translation="MSLLNDIPLTALHYYLTAPYPCSYLPEREARSQVATPTFLINTP
                     VYSRLMRMGFRRSGTHTYRAHCDACQSCISLRVEAAIFSPNRSQLRARKRHANLVATL
                     QPLEDKAEYYELYQRYQCARHKNGGMDEDDRESYRTFLLQSHIDTMLVEFREPCDPAQ
                     NRQGTLRMVSVIDLLDDGLSSVYTFYDPDLPQAQLGVFNVLWQIDLCRKLQLDFVYLG
                     YWIEASRKMAYKTQFQPAQGLVDGIWRPLDKHPPASAEAGPKESP"
     gene            complement(7556..8251)
                     /gene="aat"
                     /locus_tag="VFW59_04365"
     CDS             complement(7556..8251)
                     /gene="aat"
                     /locus_tag="VFW59_04365"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX5363478.1"
                     /translation="MIPWLTGNTPFPPVTTALAAPNGLLAAGGDLSARRLLEAYRHGI
                     FPWFSPGDPILWWSPDPRMVLVPDEFKVSRSLKKVLRNGGVEVRCDTAFERVMRACAA
                     PRPDQQGTWIQEDMIAAYRALHELGYAHSIESWREGELVGGLYGVAIGRMFYGESMFS
                     REANASKIALAHLSRQLSRWQFGLIDCQMNTPHLASLGAREIPRSEFVIRLQELVNCA
                     PVTHWHFDADLFA"
     gene            8352..9854
                     /locus_tag="VFW59_04370"
     CDS             8352..9854
                     /locus_tag="VFW59_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363479.1"
                     /translation="MLSSLFGKKSDHPMSDIKSAQALLGELPKNDAHKMLMELTEWVE
                     SVGENTDFKLDHYFAVLRLLDEAAQPCVRKLSREYFVPQALSKFQENRLWLALGNWSH
                     HAASAYFDVFERYCNGDKGAIKVPLLAARAASALRGYLKYVSAHYGPMDPQFWSKVAR
                     LYRHAEQNQYLDERIELYSGAPEGTTVRCELVRLLGWYGCGVDTLSPLYMHLTECIVG
                     QYSSSVSLTAQQSADSLFSFDLDHPAAPLRIKAGGRVLPATRFIAMATMSSRLEALLK
                     ELAKNAVPQEINLGGNYAAEVVREAAQYLLNYLTDPPLRRSPRRGINVSLKVANGFSR
                     ILDHTDAGLNFNQDQPQRWEVEDISASGFRTVLPMRGADGVRIGSLLGVQPEGVSHWG
                     VAVVRRLMCDESSRMHVGAEMLAHQVAGVTLAGGAEEEGHALWLLAKPEDSYGEVRLM
                     MKAETFAPQRSLQTRFKGKNYLLLPLRLEEQGMDYDLAGFRVVEQEGSEG"
     gene            complement(9857..10531)
                     /locus_tag="VFW59_04375"
     CDS             complement(9857..10531)
                     /locus_tag="VFW59_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5363480.1"
                     /translation="MTNPAILPHIILDSGKQPMHSIIWLHGLGADGQDFVPVADELQL
                     SAAVRYIFPHAPQRPVTINGGFVMRAWYDIAGQNIDARQDAEGIRSSQTAIEALIAQE
                     VARGIAPQDIFLAGFSQGGAIALHTALRQSVPLGGVLALSTYLPLADRAPKEMQPAAL
                     NTPIFMAHGRSDTVIPLALASASRDSLQKLGCDVKWHEYAMPHSVCPDELHDIQVWLE
                     QRLKRA"
     gene            10702..11181
                     /locus_tag="VFW59_04380"
     CDS             10702..11181
                     /locus_tag="VFW59_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5363481.1"
                     /translation="MRQNDFIVSMTDLKGRITYGNRIFIEFSGYSEEELLGSQHNIVR
                     HPDMPRAVFQLLWSKIQSREECFAYVKNMAKDGSFYWVFTNVTPIYDDAGNPNGYLSV
                     RRKPKLSGVQTMTSVYAAMLEAEKKAGPANAIAASTKLLVDILNEKGLSYDELVLAI"
     gene            11171..12625
                     /locus_tag="VFW59_04385"
     CDS             11171..12625
                     /locus_tag="VFW59_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5363482.1"
                     /translation="MQFKLATLLWSFSLGTLAIILTSFALHGVDVFMVAFLVASVALS
                     AWGQLLVKRWLAPVQKLKVVIDEVAQGKFNHRVTGIAEARDEISLLCWSVNDMLDQLN
                     TFFREQETSFRANLSGSFYRQAMSEGMHGGFSKGLENQNILLEGMAQQKIGSMRAQLL
                     SRAHSLNTENLLSNLTSNQRDLKSITDNMGELAALAKRTREDAEDSRESVKDVVQRLS
                     GIVERVNNANVAITQLNARSTEINKAVGLITSIADQTNLLALNAAIEAARAGEAGRGF
                     AVVADEVRKLAENTKNASESIGRIMQMLQGEATKMLKDSDEMHEIANSSQSVIGRLEQ
                     KFGQFYESAVTTLEGTRYAQDLSFASLVKVDHVIYKQRAYVLIDDADEIESRKAVSVD
                     HHSCRMGKWYEGVGAEVFGGSASFRSLLGPHSKVHDSVHLMVSYLGQGWERDMAMQDN
                     IYNALDAAERASQDVMEVLDRVVAEKHSHMVKAA"
     gene            complement(12665..13192)
                     /gene="yjgA"
                     /locus_tag="VFW59_04390"
     CDS             complement(12665..13192)
                     /gene="yjgA"
                     /locus_tag="VFW59_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX5363483.1"
                     /translation="MSDRQKYHDEDEDLDLPPSKTKIKQQMHELQDLGEELVALSKDQ
                     LAQLDIPDGLREAIREMNRINKFGAQRRQMQYIGKLMRDIEDTAPIVAKLNAWKGVSQ
                     QHTAHLHQLERWRDRLLENEDALTELLAAHPGIDAQRLRTLIRNAKKEKDAGKHPKNY
                     REIFQILREVIAVPE"
     gene            13278..14636
                     /gene="pmbA"
                     /locus_tag="VFW59_04395"
     CDS             13278..14636
                     /gene="pmbA"
                     /locus_tag="VFW59_04395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX5363484.1"
                     /translation="MLRSPGQTGDKFSHSADTLRDIAQDMLDYARQRGATAASAEVSV
                     GFGQSVTVRHDEVETIEYNRDKGLGITVHIGLRRGNASTSDFSRQAVRDTVDAALSIA
                     RYTAEDDCAGLPEADMLARDYPDLDLYHPWDLPVDDAIELARECEQAALQRDKRINNS
                     EGATVSVHEMQFVHANSLGFVGGYPSSRHSLSCAVIAGRDDAMERDYWYSVARDAREI
                     LRADEVGRIAAERTVRRLNARKLDTMQVPVLFEAPIASSLLGHFVGAVSGGSLYRKSS
                     FLLGQMDQPVFAPHINISDVPDIRRGLASSPFDEEGVRVQSRRIVEDGVLRGYFLSTY
                     SARKLGLRTTGNAGGNHNLILQSGELDFAGLLKQMGRGLLVTELLGQGVNQVTGDYSR
                     GAAGFWVEHGEIQYPVQEITIAGNLKDMYRNIAAVGNDVVVQGSRQCGSILVEGMTVA
                     GR"
     gene            14700..16493
                     /locus_tag="VFW59_04400"
     CDS             14700..16493
                     /locus_tag="VFW59_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX5363485.1"
                     /translation="MRPSSSSSSTEHRADWQAIRSLLPYLLEFKWRVGIALTLLVLSK
                     LANVSVPLVLKEIIDSFDASHAVLAVPVALVVAYGLLRLFSTLFGELRDAVFAKVTQR
                     AIRRVALQVFEHLHSLSLRFHLDRQTGGVSRDIERGTRGIGFLLNFMLFNILPTLLEI
                     GLVAAILFSKYNVWFAVITFVTLLIYIAFTLFITEWRMVVRRSMNDLDSKANSRAIDS
                     LLNYETVKYFGNERYEARRYDENMQRWEVAAVRNTTSLALLNAGQSFIIAIGITALML
                     LAASEVTKGLMTVGDLVLINVFMIQLYMPLHFLGFVYREIRHALADIERMFGLMHQHR
                     EIADAPDASALVVDQAEVRFERVSFGYAPDRQILFDVDFTIPAGKTVAVVGSSGAGKS
                     TLSRLLFRFYDAQSGRILINGQDIRDVTQLSLRAAIGIVPQDTVLFNDTIYYNIAYGR
                     PDATREEVIAAAQAAHIHNFVESLPQGYDSMVGERGLKLSGGEKQRVAIARAILKDPA
                     ILIFDEATSALDSKSEKAIQSELRGIAQNRTTLIIAHRLSTVVEADQILVMDNGRIVE
                     RGTHRDLLAQGRVYAQMWDLQKQEEINDSAC"
     gene            16551..17453
                     /gene="pbpG"
                     /locus_tag="VFW59_04405"
     CDS             16551..17453
                     /gene="pbpG"
                     /locus_tag="VFW59_04405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEX5363486.1"
                     /translation="MKKRLLITMLLGSTFVTQVAWAERHAHHLRLDREMSAEPQRASA
                     SPKLRSNIALILDEKTLQPLYAKNADEIAPIASITKLMTAMVVLDAGQALNEEVSVDV
                     GDLDTLKGTHSRLRIGMTFPRSELLKLALMASENRAASALARNYPGGLSAAIAAMNAK
                     ARALGMSDTQFHDPTGLNSDNVSTARDLAKMVMAARKYNLIQQYTTTATHSVDGWNGR
                     EMRFTNTNPLVRNASWDIGVSKTGFINEAGRCLVMEAKINQRPVIIVLLNSLGKSTRI
                     GDAQRIKKWIESSNTRGTATRTRG"
     gene            complement(18007..>18797)
                     /gene="proB"
                     /locus_tag="VFW59_04410"
     CDS             complement(18007..>18797)
                     /gene="proB"
                     /locus_tag="VFW59_04410"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5363487.1"
                     /translation="DMADRKRYLNARTTLRTLLGLNVIPIINENDTVVTDEIKFGDND
                     TLGALVTNLIEADALIILTDQSGLYTADPRKDPNATLVAEAQAGDATLEQMAGGAGSH
                     IGRGGMLTKIIAAKRAARSGAHTVIASGHEHDVLLRLLQGQSIGTLLTAPTLTLAARK
                     QWLADHLQVAGKLVLDTGAIKALRDEGKSLLPIGVKEVFGEFERGAAVACVDEDGRDI
                     ARGLTNYNATEARRIARHASQEIETLLGYGGDHELIHRDNLVLL"
BASE COUNT         3678 a         5201 c         5811 g         4107 t
ORIGIN      
        1 ccttgagcag gtttctcatc agggccaagt tgtccggagt gtcgtcgacc accaggatcg
       61 tggctttttt gatggaatca ggtgcgttca cggtggctcc tttaaggtag atgtcgcgca
      121 gggcgaagcc cgaagtcgaa atgcatgggc aatcccttgt ttatgatttg acttcgcggc
      181 aaggggcgcc gtcacgctca acgggaacgg gttccccctg ttagtcatct tccagtgagc
      241 tggtggcgcg tatgacttct tccagtgaag tcaatccacg caatgccttt ttgaaaccgt
      301 cataacgtag attgcgaaat ccctgttgcg tcgcgatagc caaaatctcg tcgtaactgc
      361 ggccgtgcag gatcgcttcg cgtatctgcg gagagatttt caggaattca tggatgccga
      421 cccgtccggt gtaacctgta ccgttgcaac gcttgcagcc ttttccctta tagaataccg
      481 gcagctctgc accttctagc cagaagaaat actggcgtaa ctcatcggga tcgtggcgat
      541 gttcggcctt gcaaaattcg cacagtcttt ttaccaaacg ctgccccagc acgccgatga
      601 tggagggcgc cacgatgaaa ttctccacgc ccatttcaag caagcgcgtg gtcgcctgaa
      661 tcgcatcgtt ggtatgcagc gtggtcaaca ccagatgacc ggtcaatgcc gcctgcgctg
      721 cgatgcgcgc ggtctcggtg tcgcgaacct ctcctaccag gatagcatcg ggatcttggc
      781 gcagcacgga acgcaagacg tctttgaagc tacggccaac tctgtcgtta accatcacct
      841 gattcagatc cctcacttcg tattcaatcg gatcctcgat agtgataatg ttggtggtac
      901 gcgtattgat aaagttcagg gccgcatata acgtggtgct cttgcccgat ccggtcgggc
      961 cggtcacaaa caggatgccc tgcgggcgtt tcagcgtcgc cttgaacggg cgcaacacct
     1021 ccggagagaa atccagcttg tccagattca gaatgtcgct ggaatacagc gaacccagaa
     1081 tgcgtatcac cactttttcg ccgtgcatca ccggcaggca ggaaacgcgc aaatccagca
     1141 ctttcatcgg taacgaaaaa ctgaaacggc catcctgagg cctgcgccgc tcggtgatat
     1201 ccatccccga ggtgatttta taacgcgatg tcagcggcaa cgacatccct gcgggcagga
     1261 acagcctgtc caccatatcc ccgtcaatcc ggaagcgtac caccacctca ttgcgcttcg
     1321 gttcgatatg gatatccgag gcgcgttctt tcagtgccag caggatgatc gaatcgccaa
     1381 gctcgatgag cggcttggag tcgagcagct ttgccagcgc cgcattgctc agttcggtgg
     1441 actggaatac cgacaggtcg aaactcatca ccaggtcatc cacatgctgt gccgactgat
     1501 aagttacctt gatcgccgaa ttgacctcat ccctgaaaca gaagaccggg ctgatcgact
     1561 tcattaataa attccctagc tccttgacga tcttcgcatc attgggggtg accatcccca
     1621 tggtcaccac ctggcccagc tggtagatcg gaatggcttg gtagtgttgc gcaatttcgc
     1681 ccgtcagcaa ggagaccact tcatcctgaa acagggtttt gctcaggttg acgtaagtgc
     1741 gatttatctg acttgccagc aaactccccg ccgtgtcgcg atccagatat ccatcctgaa
     1801 ttacgaattg cgcaaaatcg attgcatcat tattggcagc gctaatcatg cctgccaacg
     1861 gcacgggtgg aatatgtgcc tttttctgaa aactttcgag cagcgaatcg gtcagttcca
     1921 tcatcagaat ctctttctgc gcgtcgtgag caatttgcga atatcgtctt gcgtgacacg
     1981 cgctttgtcg gtggatccca gaaactgttt ttcttgcgcc gcctgctgcg ccaggtgatc
     2041 gagatcgaag cgctggtcca caaaaactcc ggcccgccca cttcctgccg cctgctcgat
     2101 cagattgatc ccgcattggc cgcaaaataa ttcgcccact ccggttacaa agccgcaacg
     2161 gcagcgggca tactctaatg cggccccgca tgttccgcag aaaacgtaac ctgccgggtt
     2221 ggtgtgatta cacttcatga ttagacatcc agcccgactg cttttaatgc agccatatat
     2281 tcaccccagg ttgtgctgat ctctttatgc aactcgttgg cagtcgtatt cttcaggatg
     2341 taattccatg ctccgttatc aatgcactcc tttacgagct caagcgtatt caatgaggtc
     2401 aatatcacca ccagagtttt tttgttgatg gccatgattt gtttaagcac cgcgactcca
     2461 tccatttttg gcatattgat atccagcaac accatgtccg gcgacacttg cctatacagg
     2521 cttaatgcct gctcaccatc ttctgcttcg gcgacaacct ccgcccccag tgaggtaaca
     2581 attaaactga tcaagctgcg aatatgccgt tcatcgtctg cgatcaatat gcgaaccttg
     2641 cgtgtttcca tgggcgtttc tccagattcc agtcccgatg ctaccttggc tggtttttct
     2701 ctgaactgac agcacgggcg gatcgccgac cgtgtcaccg aattcatgcg caacgggtgt
     2761 gatagtgcga cgaatggtta atcagtaatt taatgtcatc gtcagttcga gattttgtcc
     2821 gttgtacggg aagctggcag ccgagaaaga aggggcgccc aatgtgccct tcgcatcgtt
     2881 tgaaaaacca tagccttcca tcggaacccc cagccaattc gtatcgagtt tgccgttcat
     2941 gttttcgtca tgaacgacgg ccatcgcata gtttccctgc ggaatgtctt caaagtcgca
     3001 gcgcgcctgg tctttccgaa tcttgatgac catgacattc gttgccatgc gcagatattc
     3061 agtgggaaaa cctgccgaag cgtcgaataa cgcacatgca acggtcccgg tgctgttttt
     3121 aatgttcaaa attttgacgt gaatcccggg gcagtttgat tccgcaaaga cggggctggg
     3181 aagaagtccc agcgccaata ccgcaggcat aaaccaaacc atatcccgac gccttgtggt
     3241 aacgcgttcg ttgcccgcca gccttggcag aaacttgtcc ggcccctgac tcggatcgat
     3301 ttggtgctgg cctgccaaca caaccgcgtg gcgcttcagt tgctcgatgc tgaaaagctc
     3361 cgcgcgtaac aactcggtat ccccgtcgtt gccgggttta ttgcgaatgc ctgcgataaa
     3421 gctaaattct ttgatgaaat tctccttgtg cgatagcccg ggccggataa ggagtcaggt
     3481 attgcggcgg caagaagcaa tcttcgggcg acagcccgca attccattca gatgtccgcg
     3541 cagactcgat caggtagatg catgatgccc acccctgact tttcccagcg cgagtctccg
     3601 cttgcttggc attgcggtct gtgcggtaaa gaacagatct ggatgttgcg cccggataat
     3661 cgaaaagatg gtcttgtcga cggaaaggct gattcaagaa cggctgcatc ggtcggatgg
     3721 cgcagcacgg cgaatacggc agtgcattcc ggccatctgt gcgaacacga acagaacttg
     3781 tgcttttaaa cgcagttaat gttcggcatg gcaaagagca tgttgcaact tgctatccct
     3841 gaatcgggtt tccgtttttc aatcgaggag agcgatatgc tggtgacgtt cagaacaaat
     3901 gcctatccta atatctccat gtttggggat gacgcgctgg ccatgctgaa aatgatggga
     3961 cacagcgcaa cggtgcccgg ctctattcgc gcagaagatg tggcctatgc actgagtcgg
     4021 ttaaacgacg ctgtcggcaa acagaaatcc ccgccgccgg ttgccgttga aaagggggaa
     4081 gagccggcgg tcagtttggc gcatcgagcg atgcctctga tcgatctgct tgctgccgca
     4141 gtcaaagcaa acgattacgt gatgtgggat aaatcaacaa tcatcggtta aggaagaacc
     4201 tcatgaaaaa aatggaacag ctggaattgg atgcctaccg cgatgagatc ggtgcggata
     4261 tgcgcggtct gcttgaaaag taccgcgcga tattcggctg ggatattccg gagatcgacc
     4321 agcagggcgc agacaagctg attctggcgg cgatgcacaa agccctggat gacattgccg
     4381 tgtgattggc aatacgcagc cgggtagcag gcatgcgcta accgacgtgg cggttagcgc
     4441 tcattacttc tgggttttct tgccgcgcta aaccgggtcg ccgttttcgt cgagatagaa
     4501 ctcgttcagc gcgcgttcca cttcggccat gtgggtaagc accgggccgc cgcccatcat
     4561 cacgccgatg gcgcagactt ccatgatctc ttccagtgtc acgcccgcgt gtttgcaggc
     4621 gcgcacatgc agcgcgatgc agtagtggca ttgctgcgtc agcgccatgc cgagcgcgat
     4681 gcgctccttc tccttcttgt ccagcacgcc cggctgcatc gcttcggcca tgaatgcctg
     4741 caccttgccc atcagtttcg gcttgtattc ggtgagcatc tggatgccgc gatgggcgtg
     4801 ttccagcact ttgcgttcgc tgtccgaacc tttggtcttg ttggtcatgt gttctccgtt
     4861 gatgagtgag tcgaatcagt gcatggcgcg gatggcgttg gcgcattccg ccaccagcgc
     4921 cgggccgcga tagatcaggc cgctgtagac ctgcaccagc gccgcaccgg cacgcatctt
     4981 ctcgacggca tccgccccgc tcaggatgcc gcctgcgccg atgatgggca gtgtaccttg
     5041 caattcggcg gcgaggctgc ggatcaccgc agtggatttg tcgcgcaccg gtgcgccgga
     5101 caggccgccg gtctcgttgc cgtgcggcag cgtctccacg ccgtcgcgcg acagcgtggt
     5161 gttggtagcg atcacgccgt cgatgcggtg gcgcatcagc agcgcggcga tgttcctgat
     5221 ctgatcggcg tcgaggtcgg gtgcgatctt caccgctacc ggcacgtgac ggccatgctg
     5281 ttgcgccagc ttctcctgct ccgccttcag ttgcgacagc agcgcgtcga gttcgtcccc
     5341 gccctgcaac tggcgcaggt tcttggtgtt gggcgaggag atgttgacgg tgacatagct
     5401 cgcgtgggcg tacaccttgc gcaggcagat caggtaatcg tcggcggcct tttcgatcgg
     5461 ggtggcggcg ttcttgccga tgttgatgcc cagcacgccc ttgaagccgg cgcgctgcac
     5521 gttggcgatc aatgcgtcca cgccgtcgtt attgaagccc atgcggttga tgatgccctg
     5581 cgcttccggc aggcggaaca ggcgcggctt ggggttgccg ggctgcggca gcggggtgac
     5641 ggtgccgatc tcgacgaagc cgaaacccag cgcggccagt ccgttgatgc agtcgccgtt
     5701 cttgtccagt cctgcggcca ggccgaccgg attcgggaag gtcagtccca tcacggtgcg
     5761 cggatcgtcg gccggacgtc gcgccatcaa gccgttcagg cccaacgcct gtgcggcatt
     5821 caggccgtcg agggtgaggt ggtgggcggt ttccgggtcg agccggaaca gtgcggggcg
     5881 gagcaatgaa tacatggttt agcttttctt gacgacgacg ggaatcggga atttcttttt
     5941 cggcttgatc ggcgcatgac gctgcgtgcc gcgccagtgt tcggcgacga catcgccgag
     6001 ttgcaggctg agcaggccga acaggatata gccggactgc gcgagcagct ggggtttcgg
     6061 tacgccgcct gccgcctgat agatcaggat ggcgaggttg ccgacgatca gccccgccag
     6121 cgcggcgcag accggccgtc cccagttgcc gttcttcagg ccgtggaaca gggtgccttc
     6181 gtgccacagc agttgcagcg caaacagcag cagcaagggg gcggtccaca gcagccagta
     6241 ttgttgcgct ggcagagccg cgcctccgag caggccgaac gcggcgatgg cggtgagtgt
     6301 cagcgcggcg gtttcgttgg cgggcgaggg cagcggcagt cctcgtgcga gcgctttgag
     6361 caggcgcggg aacgagccga tcagcgcgcg ggtgccgagc acggccggcg ccagcagcgc
     6421 gaacagcagg gtgtcgcaca gcatggggag gaggccgccg gaggtcgtcg cccagtccgt
     6481 gccaaagcgg ctcagataaa ccagcagcca gccggtcacc gcaccggtcg cactcatggc
     6541 aagataatag ccgcgttgca tgaccagcgg attgttgccg gtgcgcagct tgaccagcag
     6601 gccggtcaac agggtgtagc ccagcagcac gagcagcgtg agggcgacgg ggtcatgggg
     6661 cgctcgtgtt tcctgagcgg cgagctcacc ccattcgtgt ccgcccagcc aaaaaccggc
     6721 gggcggtaac agcagcaggg cggcgataaa tataaacagt cgtgttttca tggggattcc
     6781 ttggggcctg cttcggcaga ggccgggggg tgtttgtcca gcggacgcca gattccatcg
     6841 acgagcccct gagccggctg gaactgggtc ttgtaggcca tcttgcggct agcttctatc
     6901 cagtaaccga gatagacaaa atccagctgg agtttgcggc acagatcgat ctgccacagc
     6961 acgttgaaca cgcccagttg cgcctgtggc agatcggggt cgtagaaggt gtataccgag
     7021 gacagtccgt cgtcgagcag atcgatcacg ctgaccatgc gcagtgtgcc ctgccgattc
     7081 tgggcagggt cgcagggttc gcgaaattcg accagcatcg tgtcgatatg gctttgcagc
     7141 agaaaagtcc ggtaggactc gcggtcatcc tcatccatgc cgccgttctt gtgacgggcg
     7201 cactggtaac gctgatagag ctcgtagtat tcggccttgt cttccagcgg ctgcagcgtg
     7261 gccaccagat tcgcatggcg ttttctggcg cgcagttgcg aacggttggg cgagaatatt
     7321 gctgcttcca cgcgcaggga gatgcagctc tggcaggcat cgcaatgggc gcgataggtg
     7381 tgcgtgccgc tgcggcgaaa gcccatccgc atcagtcttg agtagacggg ggtgttgatc
     7441 aggaaggtcg gcgtggccac ctgcgaacgc gcctctcgtt ccggcagata gctgcaggga
     7501 tagggcgccg tcagataata atgcagcgcg gtgagcggga tgtcgttaag taagctcatg
     7561 caaacaggtc ggcatcgaag tgccagtggg taactggggc gcagtttacc aattcctgca
     7621 gtctaatgac gaattcgctg cgcgggatct cgcgtgcgcc cagcgaggcg aggtgcggtg
     7681 tgttcatctg gcagtcgatc aggccgaatt gccagcggct cagttgtctg gaaaggtgcg
     7741 ccagtgcgat cttggatgcg ttggcttcgc ggctgaacat ggattcgcca tagaacatgc
     7801 ggccgatggc aacgccatac aggccgccga ccagctctcc ctcccgccag ctctcgatcg
     7861 agtgcgcgta gcccagttca tgcagcgcgc ggtatgccgc gatcatgtct tcctgtatcc
     7921 aggtcccttg ctggtcggga cgcggcgcgg cgcaggcacg catcactcgt tcaaaggcgg
     7981 tgtcgcagcg cacttcgacg cctccgttgc gcagcacttt tttcagcgaa cgcgatacct
     8041 tgaactcatc cggaaccagc accatgcgcg ggtcggggct ccaccacagg atggggtcgc
     8101 cggggctgaa ccaggggaag atgccgtgac gataggcttc cagcaggcgg cgtgcggaca
     8161 ggtcgccgcc cgccgcgagc aggccgttgg gcgcggctag cgccgttgtt acggggggga
     8221 atggagtgtt gccggtcagc cacgggatca cgtgtgcgtt tcctctctca tgcgttcatc
     8281 ggttgggcct gtgttcatca cagtgctttg cggttatatt gccacgaagc cacagcttga
     8341 cgaggttcaa tatgctatcc agtttgttcg gcaagaaatc cgatcatccg atgtcggaca
     8401 tcaagtctgc gcaggccctg ctgggggagc tgccaaaaaa tgatgcgcac aagatgctga
     8461 tggagctgac cgagtgggtg gaatcggtgg gcgagaatac cgatttcaag ctggatcatt
     8521 atttcgctgt gttgcgtttg ctcgatgagg ccgcccagcc ttgcgtgcgt aagctgtcgc
     8581 gcgaatattt tgtgccgcag gcattgagca agtttcagga gaaccggctg tggctggcgc
     8641 tggggaactg gtcgcaccat gcggccagcg cttacttcga tgtgttcgag cgttattgca
     8701 acggcgacaa gggggcgatc aaggtgccgc tgctggcggc gcgagcggca tctgcgctgc
     8761 gcggctacct gaaatacgtc agtgcccact atggcccgat ggatcctcag ttctggagca
     8821 aggtggcgcg gctctaccgg catgccgagc agaatcaata tctggatgaa cggatcgaac
     8881 tctattccgg cgctcccgag ggcacgacgg tgcgctgtga gttggtgcgt ttgttgggat
     8941 ggtatggctg tggcgtcgat acgctgagtc cgctgtacat gcacctgacc gagtgtatcg
     9001 tggggcaata cagcagcagc gtgagtctca ccgcgcagca gagcgcagac agtctgttca
     9061 gcttcgatct ggatcatcct gcggcaccgt tgcgcatcaa ggccgggggg cgggtgctcc
     9121 cggctacacg cttcatcgcc atggcgacga tgtcatccag gctggaggcc ttgctcaagg
     9181 agctggcgaa aaatgcggtt ccgcaggaaa tcaatctggg cgggaactat gcggcggagg
     9241 tggtgcgcga agccgcgcaa tacctgttga actatctgac cgacccgccg ttgcggcgca
     9301 gcccccggcg cggtatcaat gtgagtctca aggtcgcgaa tggttttagc cggatactgg
     9361 atcacaccga tgcgggcctg aatttcaatc aggatcagcc gcagcgttgg gaggtcgagg
     9421 atatcagcgc ctcgggtttc cgcaccgtgc tgccgatgag gggggcggat ggcgttcgca
     9481 tcggtagttt gcttggggtg caaccggagg gcgtgtcgca ttggggggtc gcggtggtgc
     9541 ggcgcctgat gtgcgacgag tcgagccgaa tgcacgtggg agccgagatg ttggcccatc
     9601 aagtcgccgg tgtgaccctg gccggcgggg cggaagagga ggggcatgcg ctgtggctgc
     9661 tggccaagcc agaggatagt tacggtgagg tgcgtttgat gatgaaggcc gaaacattcg
     9721 caccacagcg cagtctgcaa acccgtttca aaggaaaaaa ctacctgttg cttccgcttc
     9781 gactggaaga gcagggcatg gattacgatc tggcgggttt ccgcgtggtg gagcaggagg
     9841 gcagcgaggg ctgaccttaa gcccgcttca atcgttgctc cagccagact tggatgtcgt
     9901 gtaattcgtc cgggcagacc gaatggggca tggcgtattc gtgccatttc acgtcgcaac
     9961 cgagtttctg caaggagtct ctggaagcgc tggccagcgc gagcgggatg accgtatcgc
    10021 tgcgtccgtg cgccatgaat atcggagtgt ttaaggctgc cggctgcatc tctttgggag
    10081 ccctgtctgc cagtggcaga taggttgaaa gtgccagcac gccgcccagc ggaacgctct
    10141 ggcgcagtgc cgtgtgcaac gcgatggctc ccccctgaga aaagccggca aggaaaatgt
    10201 cctgcggcgc gatgcctcgc gcgacttcct gtgcgatcag cgcctcgatg gcggtttgcg
    10261 agctgcgtat tccttccgca tcctgtcgcg cgtcgatgtt ctgtccggcg atgtcgtacc
    10321 aggctcgcat cacgaagcct ccgttgatgg tgaccggacg ttgcggcgcg tgggggaaaa
    10381 tatatcggac ggcggcggag agttgcaact catcggcgac gggcacaaag tcctgtccgt
    10441 ctgcccccag cccatgcagc cagatgatgc tgtgcatcgg ctgtttgcct gaatcgagta
    10501 taatatgagg tagaattgcc gggttggtca tgagcgatag tcaatcaacc gactgcgtca
    10561 tcggcgatca aaataataaa agagacggct attttaaggc attacctgat tctccggtgc
    10621 aatcgctaac cggggaacgg gtgtgctagt taggggagag ggatgaaaag gcaaattact
    10681 ccgacctcgg tggagcgcgt catgaggcag aacgatttca tcgtttccat gaccgatctg
    10741 aaggggcgta tcacgtacgg aaatcgtatt tttatcgagt tttccggtta ttccgaagaa
    10801 gagttgctcg gatcgcagca caacatcgtt cgtcacccgg acatgccgcg cgcggtattc
    10861 cagttgctgt ggagcaaaat acagagccgc gaggaatgtt tcgcctatgt gaagaatatg
    10921 gcgaaagacg gcagtttcta ctgggtgttc accaacgtca cgcccatcta tgacgatgcg
    10981 ggcaatccca atggctacct gtcggtgcga cgcaagccca agctgagcgg cgtacagacc
    11041 atgacttcgg tgtatgcggc catgctggaa gcagagaaaa aagcggggcc agccaacgcc
    11101 atcgcggcgt cgaccaagtt actggtcgat attttgaatg aaaagggact cagctatgac
    11161 gaactcgtcc ttgcaattta agcttgccac cttgttgtgg tcgttttcgc tgggtacgct
    11221 ggccattatc ctgaccagct ttgcattgca tggcgtcgat gtgttcatgg tggccttcct
    11281 ggtggcgagt gtcgcgttgt cggcatgggg ccagttgctg gtgaagcgtt ggctggctcc
    11341 cgtgcaaaag ctcaaggtgg tcatcgatga agtggcgcag gggaaattca atcatcgtgt
    11401 cacgggcatc gccgaggcgc gcgacgagat cagcctgctg tgctggagcg tgaacgacat
    11461 gctcgaccag ttgaatacct tcttccgcga gcaggaaacc agtttccgcg ccaatctgtc
    11521 gggaagtttt taccgtcagg ccatgagcga ggggatgcac ggcggtttca gcaagggcct
    11581 cgagaatcag aatatcctgc tggaggggat ggctcagcag aagatcggct cgatgcgcgc
    11641 acagttgctg tcgcgggcac attcgctcaa taccgagaat ctgttgagca acctgacctc
    11701 gaatcagcgt gacttgaagt cgatcaccga caacatgggc gagttggctg cgttggcaaa
    11761 acgcacgcgc gaggatgccg aagacagtcg cgaatcggtg aaggatgtgg tgcaacgact
    11821 ctccggtatc gtcgaacgcg tgaacaacgc caacgtcgcg atcacccagt tgaatgcgcg
    11881 cagcacggag atcaacaagg cggtcgggtt gatcacctcg atcgccgatc agaccaatct
    11941 gctggcgctg aatgcggcga tcgaagcggc acgagccggt gaagccggac gcggttttgc
    12001 cgtggtggcc gatgaggtgc gcaagctggc cgaaaatacc aagaatgcct cggagtccat
    12061 tggccgcatc atgcagatgc tgcagggtga agcgaccaag atgttgaagg actcggacga
    12121 aatgcacgag atcgccaact cctcgcagag cgtcatcggc aggctggagc agaagttcgg
    12181 ccagttctac gagtccgccg tgacgacgct cgagggcacg cgctatgccc aggatctgag
    12241 tttcgcttcg ctggtcaagg tggatcacgt catttacaaa cagcgtgcct atgtattgat
    12301 cgacgatgcc gacgagatcg agagcagaaa agcggtgagc gtggatcatc acagctgccg
    12361 catgggtaaa tggtacgaag gtgtcggtgc cgaagtgttc ggtggcagcg cgagcttccg
    12421 cagcctgtta gggccgcata gcaaggttca cgatagcgta cacctgatgg tcagctatct
    12481 gggccagggc tgggagcgcg atatggcgat gcaggacaat atctataatg cgctggatgc
    12541 tgccgagagg gcgagtcagg atgtgatgga agtgctggac cgggttgtag ccgaaaagca
    12601 ctcgcacatg gtgaaggccg cctagcctgc acctctggcc gacagcgatc ggccagagca
    12661 gaggtcactc cggaacggcg atgacctcgc gcaagatctg aaagatttcg cggtaattct
    12721 tgggatgctt tccggcatcc ttttcttttt ttgcattgcg gatcagcgtg cgcaatcttt
    12781 gcgcatcgat tcccgggtgt gcggcaagca gctcggtcag cgcgtcttcg ttttccagca
    12841 gacggtcgcg ccagcgttcc agctgatgca ggtgggcggt gtgctgctgc gatacgccct
    12901 tccaggcgtt cagcttggcc acaatggggg cggtgtcttc gatgtcgcgc atcagtttgc
    12961 cgatatattg catctggcgg cgctgtgcgc cgaacttgtt gatgcggttc atctcgcgga
    13021 tcgcctcgcg caggccatcg ggtatatcga gctgagccag ctgatccttg ctcagtgcga
    13081 ccagttcttc accgagatcc tgcagctcat gcatctgctg tttgatcttg gtcttgctgg
    13141 gaggcaggtc gaggtcttca tcctcgtcgt ggtatttttg gcggtcactc atggtaggga
    13201 taggtttttt gcgatgctgc tatgataacg cctttcttca ctccaatcgt tcagttatga
    13261 gtcataccgc atcgaccatg ctccgttccc ccgggcaaac tggggacaag ttctcgcatt
    13321 ccgccgacac cctgcgtgac atcgcgcagg atatgctgga ctacgccagg cagcgcggcg
    13381 caaccgcagc ctccgccgag gtctcggtgg ggttcggaca gtccgtcacg gtgcgtcacg
    13441 acgaggtcga aaccatcgag tacaaccgcg acaaggggct gggtattacc gttcatatcg
    13501 gcctgcggcg cggcaatgcc agcacctcgg atttttcgcg gcaagcggtg cgcgacacgg
    13561 tggacgccgc cttgtccatc gcgcgctata ccgccgagga cgattgcgcc gggttgccgg
    13621 aggcggacat gctggcgcgc gattatcccg atctggatct gtatcatccg tgggacttgc
    13681 cggtggacga cgccatcgag ctggcgcgcg aatgcgagca agccgcgctg cagcgcgaca
    13741 agcgcatcaa caattcggaa ggtgcgacgg tgagcgtgca cgagatgcag ttcgtgcatg
    13801 ccaatagtct gggttttgtc ggcggctatc cctcttcgcg tcatagtctc agttgtgccg
    13861 tgatcgccgg tcgtgacgat gcgatggagc gcgattactg gtacagcgtg gcgcgcgatg
    13921 cgcgcgaaat tttgcgcgcc gatgaggtcg ggcgcatcgc cgccgaacgt accgtacgcc
    13981 gcctgaatgc gcgcaaactc gacaccatgc aggtgccggt gctgttcgag gcgccgatcg
    14041 cctccagtct gctcggtcac ttcgtcggtg cggtcagcgg aggcagcctt tatcgcaagt
    14101 cttctttcct gctggggcag atggatcagc cggtattcgc gccgcacatc aatatctctg
    14161 acgtgcctga catccgcagg gggctggcat ccagtccgtt cgacgaagag ggcgtgcgcg
    14221 tgcagtcgcg gcgcatcgtc gaagatggcg tgctgcgcgg gtattttctg agtacctatt
    14281 cggcgcgcaa actcggcctg cgcaccaccg gcaatgccgg cggcaatcac aacctgatcc
    14341 tgcagtcggg cgagctggat tttgcagggc tgctcaaaca gatggggcgc ggcctgctgg
    14401 ttaccgagtt gctcgggcag ggtgtgaatc aagtcaccgg cgattattcg cgcggcgcgg
    14461 ccggattctg ggtagagcat ggcgagatcc agtatccggt gcaggagatc accatcgcag
    14521 gcaatctcaa ggatatgtac cgcaacatcg ccgccgtcgg caacgacgtg gtggtgcagg
    14581 gttcgcgtca atgcggttcc attctggtgg aaggcatgac ggtggccggt cgctgatgct
    14641 ggcagctgcg ccgggccggt atggggctat aatgcgcccc gattaatcgc gaactagcta
    14701 tgcgcccttc ctcctcttcc tcgtctaccg agcatcgcgc cgactggcag gcgatccgtt
    14761 cgctgctgcc ctatttgctg gagttcaagt ggcgtgtggg catcgcgctg accctgctgg
    14821 tgttgtccaa gcttgccaac gtttccgtgc cgctggtgct gaaggagatc atcgattcgt
    14881 tcgatgcctc gcatgccgtg ctcgcggtgc cggtggcgct ggttgtcgct tacggtttgc
    14941 tgcggctgtt cagcacgctg ttcggcgagt tgcgcgatgc ggtgttcgcc aaggtgactc
    15001 aacgcgcgat caggcgcgtg gcattgcagg tattcgagca tctgcacagt ctcagcctgc
    15061 gcttccatct cgaccgccag accgggggcg tgtcgcgcga catcgagcga ggtacgcgcg
    15121 gtatcggctt cctgttgaac ttcatgctgt tcaatatcct gccgacgctg ctggaaatcg
    15181 ggttggtggc ggcgatcctg ttcagcaaat acaacgtatg gttcgcggtg atcaccttcg
    15241 tcacgctgct gatctacatc gcgttcacgc tgttcatcac cgagtggcgc atggtggtgc
    15301 gccgatcgat gaacgatctg gattcaaagg cgaacagccg tgcgatcgac agcctgctga
    15361 actacgagac ggtgaagtat ttcggcaacg agcgttatga agcacggcgt tacgacgaga
    15421 acatgcagcg ctgggaagtg gcggcggtgc gcaacacgac ctcgctggcg ctgctgaatg
    15481 cggggcagag ttttatcatc gccatcggca tcacggcgct gatgctgctg gctgccagcg
    15541 aagtgacgaa gggattgatg acggtgggcg atctggtgct catcaacgtg ttcatgatcc
    15601 agctgtacat gcctctgcac ttcctgggtt tcgtttaccg cgagatccgt catgcgctgg
    15661 cggatataga gaggatgttc ggtttgatgc accagcatcg cgagatcgcc gatgcgcccg
    15721 atgcttccgc tttggtggtg gatcaggctg aagttcgctt cgagcgagtc agcttcggct
    15781 atgcgcctga ccggcagatt ctgttcgatg tcgatttcac gatccctgcg ggcaagaccg
    15841 tggcggtggt gggctccagc ggcgcgggga aatcaacgtt gtcgcgcctg ctgttccgtt
    15901 tctatgatgc gcagagtgga cgcatcctga tcaacggtca ggatatccgc gatgtcacgc
    15961 aactcagtct gcgtgcggct atcggcatcg tgccgcagga tacggtgctg ttcaacgata
    16021 cgatttatta caacatcgcc tatggtcgcc cggacgcgac gcgcgaggag gtgatcgcgg
    16081 cggcacaggc tgcgcacatc cataacttcg tcgaatcgct gccgcagggg tatgacagca
    16141 tggtgggcga acgcgggctg aagctctccg gtggcgaaaa acaacgggtg gcgatcgcgc
    16201 gtgccatcct gaaggatccg gcgatcctga ttttcgatga ggcgacttcg gcgctcgatt
    16261 cgaaatcgga gaaggcgatc cagtctgagc tgcgcggcat tgcacagaat cgcaccacgc
    16321 tgatcatcgc ccatcgcctg tctacggtgg tcgaggccga tcagattctg gtgatggaca
    16381 acggccgcat cgtcgagcgc ggcacgcatc gcgacttgct ggcgcagggg cgggtctatg
    16441 cgcagatgtg ggatcttcag aaacaggaag agataaatga ctctgcctgt tgatttatat
    16501 catttttggg gtatcctacg ggctgccgaa acctgtttca gggagttgcc atgaaaaaaa
    16561 gattgctgat aaccatgctg ctgggtagca ccttcgtcac tcaagtggct tgggcggagc
    16621 gccatgcgca tcacctccgg ctggaccgcg aaatgtctgc cgaacctcag cgcgcatccg
    16681 cctcgccgaa gctgcgttcc aatatcgcat tgattctgga cgaaaagacg ctgcaaccgc
    16741 tgtatgccaa gaatgccgat gagatcgctc cgattgcctc tatcaccaag ctgatgacgg
    16801 cgatggtggt gctggacgcg gggcaggcgc tgaacgagga ggtgagcgtc gacgtggggg
    16861 atctggatac gctcaagggc acccattcgc ggctgcgcat cggcatgact ttcccccgca
    16921 gcgaattgct caagctggcg cttatggctt cggagaatcg ggcggcctcg gcactggcgc
    16981 gtaactatcc cggtgggttg tctgcggcca ttgcggcgat gaatgccaag gcgcgcgcgc
    17041 tgggcatgag cgatacgcag ttccatgatc caaccggcct gaatagcgac aatgtttcga
    17101 cggcgcgcga tctggcgaaa atggtcatgg cggcgagaaa atacaacctg attcagcaat
    17161 acaccacgac ggcgacgcat tccgtggatg gctggaacgg gcgcgagatg cgtttcacca
    17221 acaccaaccc gctggtaagg aatgcctcct gggatatcgg cgtcagcaag accggcttca
    17281 tcaacgaggc gggtcgttgt ctggtgatgg aagcgaagat caaccagcgt ccggtgatca
    17341 tcgtgctgct caattcattg ggcaagagca cgcgtatcgg cgatgcccag cgcatcaaga
    17401 aatggattga gagcagcaac acccgcggca cggcgacacg cacacgcggc tgatcgatgc
    17461 tgatcgaact ggcgatcctg atcgtgctgg tcgcggtcgt ggtgatcgcg ctgcgcggct
    17521 agtcgctggg ctttttttgc agaggtggtg aagacctcct ctgcaatatt tcccgccacg
    17581 atactttcct tgccaagccg cttggctatg cagagctcac atcagctacg ctgatatgag
    17641 cctatgccgc aatctagtct tgttgtttca taacagaaat tgtggcttca gccgccttgc
    17701 ggcttaacat cagcaaagct gatgttagct tccgccgtaa cttagtgttg ctgtaattac
    17761 tgcaacacta gttgtggctt cggccgctgc tgagcagctt aacgttcgct acgctcacac
    17821 aacacgccgc aacgcggctt gttgcggcga ggacttacct tcaggttatg tcgaagccaa
    17881 tagccttcac cgcaacttag tcctgttgtt tcacaacaga ggaggttgtg gttccggccg
    17941 ccgctacgca gcttcacatc agctgcgctg atatgagcca gcaccgcaat ctggtcttgc
    18001 tgtgtttcaa agcaagacca gattgtctct atggatcagc tcgtggtcgc cgccgtagcc
    18061 cagcagggtt tcgatttcct ggctggcgtg gcgtgcgatg cggcgggctt cggtggcgtt
    18121 gtagttggtc aggccgcggg cgatgtcgcg gccatcttcg tcgacgcagg cgactgctgc
    18181 gccgcgttcg aattcgccga atacctcttt gacgccgata ggcaacaggc tcttgccttc
    18241 atcccgcagg gccttgatcg cgcccgtatc cagcaccagt ttgccggcca cttgcaggtg
    18301 gtcggcaagc cattgcttgc gtgctgccag cgtgagggtg ggcgcggtga gcagtgtgcc
    18361 gatggattgg ccctgcagca ggcgcagcag cacatcgtgt tcgtgcccgg aggcgatgac
    18421 ggtgtgcgcg ccgctgcgtg ccgcgcgttt tgccgcgatg atcttggtga gcatgccgcc
    18481 gcgaccgatg tggctgcctg cgccgcctgc catctgttca agcgtcgcat cgcctgcttg
    18541 tgcctcggcc accagcgtgg cattcgggtc tttgcgcgga tcggcggtgt acaggccgct
    18601 ctggtcggtc aggatgatca gtgcatcggc ttcaatcaga ttggtgacca gtgcgcccag
    18661 cgtgtcgttg tcgccgaact tgatctcgtc ggtgacgacg gtgtcgttct cgttgatgat
    18721 cggaatcacg ttcagcccga gcagcgtgcg caacgtcgtc cgcgcgttga gatagcgctt
    18781 gcgatccgcc atgtcgt
//
LOCUS       DASUIV010000117         2724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_957385, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000117 DASUIV010000000
VERSION     DASUIV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957385"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1638
                     /locus_tag="VFW59_04415"
     CDS             145..1638
                     /locus_tag="VFW59_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5363488.1"
                     /translation="MSLAVLYSRALSGMDAALVTVEVHLANGLPSFTIVGLPETEVKE
                     SKDRVRAALQNCQFDFPARRITVNLAPADLPKESGRFDLPIALGILAATGQIPADRLN
                     DYEFAGELALTGELRPIRGALAMTYRAVSDGRAFMLPQANAAEAALVENATVYPAQTL
                     LQVAAHLADRERISPHASEPQTVAPQYADMREVKGQARAKRALEIAAAGGHSVLMMGP
                     PGTGKSMLATRFPGILPQMTEAEALQSAAVQSLDGSFDLSKWKTRPYRSPHHTASGVA
                     LVGGGSNPRPGEVSMALHGVLFLDELPEFDRSVLEVLREPLESGHITISRAAHRADFP
                     AQFQLVAAMNPCPCGYLGHFNGKCRCTPDQIARYRSKISGPLLDRIDIQIEVPAVPQD
                     DLFDKAEGEASSAVQARVEAARQRQLTRQDKANAQLSVGEIDVMCAPDAQGEALLRQA
                     ITRLNLSARAYHRVLKVARTIADLEGSPDILTAHIAEAVQYRRMDKS"
     gene            1770..2231
                     /locus_tag="VFW59_04420"
     CDS             1770..2231
                     /locus_tag="VFW59_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEX5363489.1"
                     /translation="MLEQKLITGMKNFNQLIHVLLALALGVACVMVIWDFGVKAAEAW
                     QAGNAASGFFHALGSLFILWTLSTLISAEMTYLQTGRIYVRVFVEVAMISVIREMIVQ
                     PVQVISAGQTESDFNPVHYGLLLAALLVVGVVYRLISDSPQQEPATQEEKH"
     gene            2380..>2724
                     /locus_tag="VFW59_04425"
     CDS             2380..>2724
                     /locus_tag="VFW59_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363490.1"
                     /translation="MDARWIEALINQAVPALATLIAAFVGAWLAFLLTDRAHVRLLKN
                     QNAAAVNQALFTMLRQYNALKNVQDQLVDPVRNDPGKFIKMQSLAPLAYATLHPEFDS
                     LSFLLETEQRGLL"
BASE COUNT          540 a          903 c          799 g          482 t
ORIGIN      
        1 gatgctgcgg gctttcgtca ttcccgcgca ggcgggaatc cagcaaaaca aatactccgc
       61 gcagcggacc gagccagata ttgtcccgcg ttgcggggct gctttaactg actggattcc
      121 cgcctatgcg ggaatgacgg actgatgagc ctcgcggtcc tctatagccg cgcactctcc
      181 ggcatggatg ccgcactggt caccgtcgag gtgcacctgg ccaacggcct gccgagcttc
      241 accatcgtcg gcctgccgga aaccgaggtg aaagaaagca aggaccgcgt gcgcgccgcg
      301 ctgcagaact gccagttcga ttttcccgcc cgccgcatca ccgtcaattt agcgcccgcc
      361 gacctgccca aggaaagcgg gcgtttcgat ctgcccatcg cgctgggcat cctcgccgcc
      421 accggacaga tccccgccga ccgcctgaac gattatgaat ttgccggaga gctcgcgctg
      481 accggcgagc tgcgcccgat acgcggcgcg ctggcgatga cctaccgcgc ggtgagcgac
      541 ggacgcgcct tcatgctgcc gcaggcgaac gccgccgagg ccgcgctggt ggagaacgcc
      601 acggtgtatc cggcgcaaac cctgctgcag gtcgccgccc atctcgccga ccgggaaagg
      661 atatcgcccc atgccagcga accgcagacc gtcgcaccgc aatacgcgga catgcgcgag
      721 gtcaaaggac aggcgcgggc caagcgcgcg ctggagatcg ccgctgcagg cggacacagc
      781 gtgctgatga tgggcccgcc cggcaccgga aaatccatgc tggcgacgcg tttccccggc
      841 atcctgccgc agatgaccga ggccgaagcc ttgcagagcg cggcggtgca atcgctggac
      901 ggcagcttcg acctgtcgaa atggaagacc cggccctacc gctcgccgca ccacaccgct
      961 tccggcgtcg cgctggtcgg cggcggcagc aacccgcgcc ccggcgaagt gtcgatggcg
     1021 ctgcacggcg tgctgttcct cgacgagctg cccgagttcg accgcagcgt gctggaagtg
     1081 ctgcgcgagc cgctggaatc gggccacatc accatctccc gcgccgccca ccgcgccgat
     1141 ttccccgcgc agttccagct ggttgccgcg atgaacccct gcccctgcgg ctatctcggc
     1201 cacttcaacg gcaaatgccg ctgcacgccg gatcagatcg cgcgctaccg cagcaagatt
     1261 tccggcccgc tgctggaccg catcgacatc cagatcgaag tccccgccgt gccgcaggac
     1321 gacctgttcg acaaggccga aggcgaagcc agcagcgccg tccaggcccg cgtcgaagcc
     1381 gcgcggcaac gccagctcac gagacaggac aaggccaacg cgcagctctc cgtcggcgaa
     1441 atcgacgtga tgtgcgcccc ggatgcgcag ggcgaagcgc tgctgcgcca ggccatcacc
     1501 cggttaaacc tctccgcccg cgcctaccac cgcgtgctca aggtcgcccg caccatcgcc
     1561 gatctggaag gcagccccga catcctcacc gcccatatcg ccgaggcggt gcagtaccgg
     1621 cgcatggata aaagctaaca tccttgccag cttagccaga tggccaaggg cttcatcaga
     1681 tttgattccg gcgttcattc cggcttgaga agtaatctgt gcgaatctgc ggataaacgc
     1741 ttttgtggtt aatacatcga aaggacagca tgctcgaaca gaaactgatc accggcatga
     1801 aaaacttcaa ccagctcatc cacgtgctgc tcgcactggc tctcggcgtc gcctgcgtga
     1861 tggtgatctg ggatttcggc gtaaaggccg ccgaagcctg gcaggccggc aatgccgcca
     1921 gcggcttctt tcatgcgctg ggctcgctgt tcatcctgtg gacgctctcc acgctgatct
     1981 ccgccgagat gacctatctg caaaccggcc gcatctacgt gcgggtgttc gtcgaagtcg
     2041 cgatgatctc ggtgatccgc gagatgatcg tgcaaccggt acaggtcatc agcgccggac
     2101 agaccgaaag cgacttcaac ccggttcact acgggctgct gctggcggca ctgctggtgg
     2161 tcggcgtcgt ctaccggctg atcagcgact cgccgcagca ggagcccgca acgcaggaag
     2221 agaaacactg acgattttta tagaaaacct acctgtattg gcaggtgtgc gtttcggcgg
     2281 ggcgggttag catccgacag cgctgcaccc gccgaattcc gaggtgtact ttcccggcat
     2341 tttttagggc gactacttta cgttaggtac tgaggccaaa tggacgcacg ttggatcgaa
     2401 gcattaatca accaggcggt gcctgcattg gcgacgttaa tcgctgcgtt cgttggcgcg
     2461 tggcttgcct ttctcctcac agatagagca catgttcgcc tactaaaaaa ccagaacgct
     2521 gccgccgtga accaagctct tttcaccatg ctccggcagt acaacgcgct gaaaaacgta
     2581 caggatcaat tagttgatcc tgtacggaat gaccctggga aattcataaa gatgcaatca
     2641 ctggcacctt tggcttacgc cacgttacac cccgagttcg acagtctctc gttcttgctt
     2701 gaaacagagc aacgaggact tctc
//
LOCUS       DASUIV010000118        26606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_308306, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000118 DASUIV010000000
VERSION     DASUIV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 26606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26606
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308306"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2586)
                     /locus_tag="VFW59_04430"
     CDS             complement(<1..2586)
                     /locus_tag="VFW59_04430"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX5363491.1"
                     /translation="MNRAALRGLGKPLLLALAYFVTAQLGFTFATAYYHITLFWLPTG
                     IAVAALLRWKTSSSMTGIWLGALLIELSLGYPILLAGGIATGMALAPLLTYWLLQRWQ
                     FESSFNRQRDLFALIAAAALGSLVSASTGVSLLWLENLLQANNAPIAWLNWWLGDALG
                     ILLAGPLLLSFTPASYKEFLRQPVESLLCLLLLGIISWLVFFADYRDHLPPLAFLPLP
                     LVIWAALRQGITGTSLAVLLLSAFTAIGTALGKGVFGVMPADEAMYLAWLYMFTIVLT
                     GLMVTTILGERKKTEKALQHTNELLSLAQRKAKAGVWDWELSSNKLAWSDELFLLFGL
                     DPKTSEASFATWLNLIHPDDRQIAEENVNAALANDTPLYNEYRIVLPDGETKWITAVG
                     NTSHYETPHMSGLCIDVTPQKEAQQRARQSELRYKTLIEHAADALFVHDFNGDFVEVN
                     QQACDSLGYSREELLRMNVADTSPHFDPKKGRALWHQLKIGQPHTLISSKRRKDGSTF
                     PAEIRFALLELDGEKRVMTLVSDITTRIEQDEQLRNTLRQLEVKELSKTRFLAAAGHD
                     LRQPIAAANLFLDTLKLTSPTPHQSQLINKLDQSMGIFSSQLERLLDISRFDSGLIKP
                     EIGSFNLAEMFTWLEHNFAESAFNKQLRFKLCFPMNRSLIVRTDIGLLESVLMNLVSN
                     ALKFTARGGILISARPRQDSVLLQVWDTGVGISEADLPHIFDEFYQAANPHRNREAGL
                     GLGLSIGQRAMSLLGGSITCRSRPGRGSVFELSLPRNGEPHRPETIPAGMPAPAISSQ
                     NQFQGKRVVVLEDDELVSHALICLLQESGAEVRHFSDAEVALQHPDIADSDFYIVDYS
                     LGPGLT"
     gene            complement(2678..5959)
                     /locus_tag="VFW59_04435"
     CDS             complement(2678..5959)
                     /locus_tag="VFW59_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5363492.1"
                     /translation="MKKALLSEILTASVKTVSPDSTISEVLKEMAALKISCVVAVDAG
                     NKPLGIFTERDAVRLLAERRGLGDVRMNEVMSTPPFCSSANIDFREAYRLLQERGFRH
                     LVVVDENGCLEGIVTEGDFLHHLDAGDLSEFKIAEKVMSRNIVTVDINDTLADAIGLM
                     SRNRYSCVVVTRERAAFAILTERDVVKLASTIADVGNLPVSGVAHTPLITVAPHTTLP
                     DAIRKMDSHQIRHLVVTDQGQLLGLLTRHDLVKTLQGGYVHFLHETIQIQRKELFKLG
                     QQRSLFKLHDAALAAADNAIVIADRQAVIQWANPAFSQLTGYTLEESVGRTIRELVRS
                     GEHGAQFYERLWNTILDGRVWRGEIINRRKDGTRYPEEMTITPVRLDGKEITHFIAVK
                     QNIDTRKQTEAELGSLNERMSALIEAIPDAIFFKDGDGRWQLTNDAAKTLFQLHGIDW
                     QGKTDMQLAELRPDCRQALDACMLNDELAWQAGGLTLFNENVSDERGEPRHFEVRKVP
                     IFDRENRRTGLVIIGRDVTAAKQTEEQLREAAAVMRHTHEGVVITDTSPKILAVNDAY
                     INITGYSAEEIIGKNPSVLSAGRESKEFYQAMWRDILEYGHWHGEVWNRRKSGEIYPQ
                     LLNISTVYDDHQAPVRYIGVFSDITHIKENQAQLEFMAHHDPLTQLPNRVLIETLLQQ
                     DLDQAHRHTLLSGVLFIDLDHFKPVNDSFGHLVGDELLQAVAKRIGARQREGDTLGRL
                     GGDEFILLLSLLHDPQDAAVVASDLIAALSEPFQLSGGHEIFIGGSIGISLSPQDGES
                     VSELMKNADAAMYLAKENGRNQFSFYTPELNADARNNLTLENALRRALQQSELTLHYQ
                     PKVDLRSGRIIGAEALARWQQPDGNWISPAQFIPIAEKSSLILDMGNWVIEQSCRQVR
                     FWLDQGLDDICIAINISARQFRSGNLDQQLSRAFERHGIAARYLEIELTESMLMQEPK
                     RAIDTMHKLKQLGLKISLDDFGTGYSSLGYLSRFPIDTLKIDQSFVRGVVTDPDDAEI
                     ASAIIGLAHRMKLRVVAEGVETPEQLAFLRSNHCDELQGYYFSKPLPADDFAALVRKG
                     KTLTS"
     gene            complement(6092..6877)
                     /locus_tag="VFW59_04440"
     CDS             complement(6092..6877)
                     /locus_tag="VFW59_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5363493.1"
                     /translation="MLKTGKFAALAFIGALAAQPAFAEEKSAAMVNGTSIPQARVDMR
                     AKAAAAQGQPDSPELRKMIREDLINLEVMAQEATKLGLNKNDDVVQEIELSKQSVLVR
                     AFVQDYTKKHPISEDQLKQEYDKLKAKLGNKEFSARHILVATQDEAKAIIAQLGKKAK
                     FEKLAEKSMDSGSAHTGGALGWAVPSNFVPPFAAALQNLKKGEYTKEPVESQFGWHVI
                     KLDDTRDLKVPPFEELRPQLQQRLQQQAIQQAIIDLKAKAKIE"
     gene            complement(6906..7169)
                     /locus_tag="VFW59_04445"
     CDS             complement(6906..7169)
                     /locus_tag="VFW59_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX5363494.1"
                     /translation="MSRITAMRTRLAALEPVALEIIDDSHKHAGHAGARDGGGHYALK
                     ITSAQFVGKNTVARHRMIYSALGEMMRHEIHALNIQAKTPDEI"
     gene            complement(7166..7468)
                     /locus_tag="VFW59_04450"
     CDS             complement(7166..7468)
                     /locus_tag="VFW59_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5363495.1"
                     /translation="MWYVITGTDVPDSLEKRLAARPAHLARLQALQNEGRLLLAGPFP
                     AVDAADPGAAGYTGSLIVAEFASLHAAQQWADADPYVAGGVYAEVRVTPFRKVFPA"
     gene            complement(7615..8157)
                     /locus_tag="VFW59_04455"
     CDS             complement(7615..8157)
                     /locus_tag="VFW59_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEX5363496.1"
                     /translation="MKLLFDLFPVILFFIAFKAFNIYTATAVAMVATLAQLIWSKYRH
                     GKIDAMLWVSFAIITLFGGATLFLHDESFIKWKPTVLYWLFSVVLLFSNLLFKKNLIR
                     SLLQEKVALPLHVWQRLNLIWSLFFAALGMLNLYIAFNYSTDIWVNFKLFGFTALMVV
                     FILGQSTWLSKYVDEKKENN"
     gene            complement(8183..8575)
                     /gene="msrB"
                     /locus_tag="VFW59_04460"
     CDS             complement(8183..8575)
                     /gene="msrB"
                     /locus_tag="VFW59_04460"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5363497.1"
                     /translation="MSKITKTDDEWRDELTPEQYRICRERGTEAAFSGAYWDCHDEGL
                     YRCVCCGNALFDSEAKFESGCGWPSFTQPYSADNVYALADNTHGMERIEVRCKQCDAH
                     LGHIFPDGPPPSGLRFCINSAALALDRK"
     gene            complement(8578..9186)
                     /locus_tag="VFW59_04465"
     CDS             complement(8578..9186)
                     /locus_tag="VFW59_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5363498.1"
                     /translation="MKLLGTDTSPYVRKVRLVLLEKNIPHEYLVDAPRDPGSHVAQIN
                     PLGRIPALILDDDSCVFDSPVIAEYLDTLNDHPILIPRNDALARMRVRRWEGLADGIL
                     DSALVVRTESLRAPEKQDANVHTLHYTAITRALAYAADLLGEREWCEGDALTLADLAL
                     VSTLIYLDLRQPDRDWRGSHPNLSAWFERIAARPSVRSTLNT"
     gene            complement(9326..10777)
                     /locus_tag="VFW59_04470"
     CDS             complement(9326..10777)
                     /locus_tag="VFW59_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5363499.1"
                     /translation="MPTNTRRRTVRKEVSTVQKVSGETKLFVLDTNVLLHDPTSLFRF
                     EEHDICLPMFVLEELDNKKKGMTEVARNARQASRFLDEIVSDAPHNIEAGIALESQAH
                     KNCTGRLFLQTSFIKHELPQNMELGKADNAILGVVIGMQQEHPNRSVILVSKDINMRI
                     KARALGLEAQDYFNDKVLEDTDLLYSGVLQLPDDFWESHGRDMKSWVKETHTYYQIRG
                     PLCGAMFVNQFVFQETNGATPFYAIVVEQDDQSALLRTLTDYTHSKNGIWGITARNRE
                     QNFVLNLLMDPEIDFVTLLGQAGTGKTLLTLAAGLMQTMETKLYTEIIITRVTVPVGE
                     DIGFLPGTEEEKMTPWMGALEDNMDVLNKNSEEAGDWGREATRDLIRSRVKVKSLNFM
                     RGRTFLNKYLIIDEAQNLTPKQMKTLVTRAGPGTKVVCMGNIAQIDTPYLTEGSSGLT
                     YVVDRFKGWQHGGHVTLMRGERSRLADYAGEVL"
     gene            complement(10787..11245)
                     /locus_tag="VFW59_04475"
     CDS             complement(10787..11245)
                     /locus_tag="VFW59_04475"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5363500.1"
                     /translation="MPQSSIADFSLPATGGQTFTLSAARGKHLVIYFYPKDNTPGCTT
                     EAIQFRDLHAAFARANCEVVGISRDSIKSHENFKAKFTLPFELLSDEEEIACELFGVI
                     KQKMMYGKQVRGIERSTFVFDKNGELRREWRKLKADGHAQEVLDFVNSLD"
     gene            11248..11625
                     /locus_tag="VFW59_04480"
     CDS             11248..11625
                     /locus_tag="VFW59_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363501.1"
                     /translation="MDSVNDGCILVKKSLKGKQRYRDAAKPLSIQGVMRWRDSEGMPL
                     PMGLTRRAEVPDSRPPEKRLDFSTCLVGFLAARLQLCFFGAVFENMISGLSGGHEVGA
                     SGLRCRRTGSSVLNPVKIQPNRF"
     gene            11747..12073
                     /locus_tag="VFW59_04485"
     CDS             11747..12073
                     /locus_tag="VFW59_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5363502.1"
                     /translation="MTAIDRNQVCDIFLGKVAALPDGRISTLIDQPDASPLREEFYMK
                     VANKTAAQTRAHWAKLYFTGRGVPPRQAQNSAEVIKMIHSKPGAIGYVERSSLDSSVK
                     VIYVAP"
     gene            12101..13291
                     /locus_tag="VFW59_04490"
     CDS             12101..13291
                     /locus_tag="VFW59_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363503.1"
                     /translation="MNDPLLKILMLMFVLLTTRSHADEGSGLEFSGSGFLTASVGKMM
                     GGTTGNVGDYNCPCFVSDYAQAGIFDGRRSLQWGPDSKLGLQGQVSFDNQKFSVTGQV
                     VARGASNGRADLEWLYGTYKLNDKVVLQAGRKRLPMFYFSDAQDIGVALPWTHLPTWL
                     YGWQAVNYNGINLGYRDQFGDWAASANLLAGDEHRKNSGYWKVYGNGRQSVTDVNWTN
                     IAGGDLTLSRDWLETRVVYLQSDTQDVNVNGTWNYASLNYDPPTGISPIAKQKIYGLT
                     VKADYENWLFFNEFIYIDHPGLTYRDFAQIVSVGYRQGKWMPMATWGHYRGAVVSQGV
                     LPGAPASVANSQQTVTLSLRYDLTTTSDLKLQFDDTSDNSEPGFNPRYGSSRLLTLAY
                     DLMF"
     gene            13297..15819
                     /locus_tag="VFW59_04495"
     CDS             13297..15819
                     /locus_tag="VFW59_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5363504.1"
                     /translation="MTVLRWIIRYRRSYLAFSALLVLLVVSVVVWRSNLDLEKKRELP
                     LARSLATLASTIESGTINSQAMGAAILLGLEHREVKQLAAGALPPDAPIVLSGLDAIR
                     EQFTANAVFLVNEQGLVVADSREGGRSTTGLDMSSWPCIQLAIQGTPNVYPVMGGAES
                     DRGIYLAAPLRASGSRASRAIGAVVVRVGADKLDMLLESWTDGIALLSSPQREVFSAN
                     RLDWLFGRTTVNRVADAHRAGRAGVAQTGYPRLSFSLEAPETTIEGVRYAVRSQLLEW
                     NDPAGDWRLVLLGRTVPWWTDGRVLSLAGLAGLLVALLLFWFYTLARHENELLKSDAL
                     LKESQNIAALGSYVLDTQTGVFEVSGETDVLFGIDGTYDHSVAGWLALIHPDDRAMME
                     RYFNDQITGQSPSFDREYRILRYNDQQERWVRGLGRMIFDSAGRPLKMHGTIQDVTDR
                     KQAEQVLNESIRQLKEKELAKTRFLAAAGHDLRQPLAAANLFIDALKFTGPTPDQNKI
                     IQRLDQAMGTFNGLLEALLNISKLDAGVIKPEYTPIQVPKLLDWLEQSFSAMAHEKGL
                     GFKTRSSMKEALVICSDLDLLKSVLMNLVSNAIKFTTSGAILVSARRRGEEVLFQVWD
                     TGIGIDPEHLENIFDEFYQINNPQRDRTSGLGLGLAIVNRALMLLGVEVTCRSKIGIG
                     SVFGFRLPLARASDMETHQSATVTSRNEAIDELFARGKRFIVVEDDRLVAQAMVNCLE
                     AMGGEVVYFKNAEEALQHANIEFGDYYIADYMLGGRLNGIQLLNQLRQRMGRHIKAVL
                     LTGDTSPAFIREAEDCNWPVLHKPVNTSRLISSLSAQQESAR"
     gene            15963..16130
                     /locus_tag="VFW59_04500"
     CDS             15963..16130
                     /locus_tag="VFW59_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363505.1"
                     /translation="MNEAVNEPVKVVQFIIREGDVADLCLDQNGNVYKVEYSGRNELT
                     LIKQTILVVHP"
     gene            complement(16187..17506)
                     /locus_tag="VFW59_04505"
     CDS             complement(16187..17506)
                     /locus_tag="VFW59_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5363506.1"
                     /translation="MPNSNLDKTDKLNEPHSPLIEDIDNYRITRVLGHGATSTVYLGV
                     DTTSLKLVAIKKIRPECSIGIQHKMFEVEASLCGKLKHPNIVSLIEANVNDVTGSYII
                     MEYVEGQSLENFSTPDKLLAVETVMDIVRQAAEALNYAFQRGVIHRDVKPANIILRVD
                     GLVKLSDFGCALLFDSETTQITGAGSLSYMSPEQIRGTALNHQSDIYSMGAVMYRLLT
                     GHNTFKAADHYAAINQILNHPYIPMEIHRVGLPQEINRIVDRALQKNLEDRYQDWKEF
                     LSDLYAASGVNRVESLVDDQTMFELMGRSAFFKDFLTVEIWEVLHASQWRTFKHNEEI
                     IKDGEHGFSFYILLQGSVVVSKRQRMLNIIQAGDCVGENACLYNGSPIRGATVSAQGD
                     VIALEVSRKQLEGFSKDVCIRMDRAFLRSLNEKLSLSNARVLQLMNL"
     gene            17725..19326
                     /locus_tag="VFW59_04510"
     CDS             17725..19326
                     /locus_tag="VFW59_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX5363507.1"
                     /translation="MVKQLVVVSGLFLQLLMACPAFAESIKFEVTGFSVEGNTLLPAE
                     QVQSALAPFVGADREMADVNKAAEALHALYQNAGYAVVQVIPPQQIITSGNVLLKVVE
                     DKIVSIEVTGNKAYSAENIRASLPALKPGASLNAKRLESGIVLANENSAKQVAVNVQP
                     GAKLGDINARIDVTEDRIEKYIGTIDNTGSAATGFTKIGFAYQNANLFDRDHALTLQY
                     SGSLDMIDKVYSLSAGYHVPLYSHNSSVDLIAAYSSSSTTTPSGAGSIYFSGQGSILG
                     ARLNYALPSVGEIQHKVILGADYKDTTNQFSGCVAPCGSITEIPLSLTYFAQANNPGF
                     QGSGNVSWLQNISGGPHGGLADYQASRFVYPGISAKPTWSLLRFNATGAVPVAQDWQA
                     RAQLNGQYTNEMLIGAEQLGAGGASSVRGYPERVAAGDYGYVVNLELYTPDMNKLVNL
                     SNGSLRALVFWDAASLTVNDQWGPAISRNTNFDSVGVGARLMYKKDLSIKLDVGWAQK
                     ALPGAAPSPVKQNDVAGHVAVAYIF"
     gene            19359..24350
                     /locus_tag="VFW59_04515"
     CDS             19359..24350
                     /locus_tag="VFW59_04515"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX5363508.1"
                     /translation="MRASKKSLPVFRLKLVALAIAACFSPCAFANPVGPSVVNGAASF
                     AVNGNTLTVTNTPGAIVNWQQFNIGAGQTTQFIQQSAQSSVLNRVIGPDPSQILGILQ
                     SNGRVYLINPSGILFGAGAVVDVGGLIASTLNITNADFLMNRMSFNGNSGTSVVNQGT
                     ITTPFGGSVYLIGNNVSNEGVITTPQGAVVLAAGNSVSMVNTVTPHVSVTVSAPAGGQ
                     AVNLGQVTAQGGSINIYASLIKQQGLLSADSASVNAQGQIVLSASQTVEVAAGSSTSA
                     ANSNGVGGTVQILGNQVNVAGAVDASGSAGGGTILVGGDFQGKNAAVQNAANTTVAST
                     ATLKADALVNGNGGKVVVWADGTTSYNGAITAKGGALGGNGGNVEVSGKGVLDFKGTV
                     NTLAPNGTGGSLLLDPFALCISSTACAYWDVVTGTYTSPSSSMTGLTLKALVAANGAT
                     TVRTSGYGGGHLLVSDSIVFTPGELSGGKTLTLQADNGELAFRNTTIDATGGSGALNL
                     NLISTGHPAYGGWIGIDSSTIMTNGGNIYINAPGGVSIGTGYVNGPTAASTVATAGGN
                     ISIVAANVEVGSPWSTGTNISTGNGNVSITTNAVFGGGNSASILAGKSMVVYGGTAVN
                     WGTGSLVVNGVTDPISSGAGALTIPSNQYWGANTNGALPLSSFAGNFSGTSIVDPMAG
                     GGTMTWSITVNATGQVTMTGSGPNTNNQGIGQLYPLSNGTFMIHFPGGLTVWGSVDPA
                     TGTLTGIWGVNGNTLGQQGSVPQGMNWGTFTSVAGGGMLQQLCTLNPALCSPTVITPP
                     TPPVNPGTSILDNNTAIADLTFSGGLNAEGGTMWITYDDVRESRDEARKAEREKDAAE
                     ERVKTAKTPKEKEDAKKHLASKTAEVELKKADASIRETESEVRAANEEVRAAKTPEDR
                     MRAENRKSVAESKRAEAEVKKADAEVKQAEAEAKHAEGPAARAAAESKKQAAESKRAE
                     AEVKKAEVDAKKAETELKQVEAAAGANEVPQAEVETKKAEVAVMKAEAEMKQAEAEAK
                     TAKDPEAKALAETKLAAAEVKKAQAEAKQAEAEVKQEEAKTGDAGVPADQRKVEAKKA
                     EAEVKQAEAETKQAKLEEKSAKDPEAKSKSEETVAKAEAKQAEAEAKAAKIKADEAKD
                     SPEARHEAEAKQANAEAKKAEVEAKTARDDGGRSQAEAKRAAAEAKRAESEEQVAREE
                     VKRARDEEREAKTPESKYVASKKIEASEARAAAKREEAAVKRAESEMKRVEAEVKQAE
                     IDVKEAATPEQRASAEKRIAEKRNEFATKRGEVAEKSRRAESVKELAESKESEFKETK
                     QRRDDIAETAFAGIDVKAMSKDRVQDAMAARHEFMKDTLAPALTLLKRDPNAGIKACV
                     EGGGEVCIKSSSQVPAYIANPAVEVRPRVQLPPLAVPAVSFLPQIQRKIAVVVGNNAY
                     QDPNLPSLNGAIRDADAIANVFQEKMGYEVRVVHNGTRADIISALNKVADEVGSNDSV
                     VLYYAGHGYQDDATKVGYWIPSDASTRNADNWITSSEINKMLTNIPAKQLMLVSDSCY
                     SGALTNEQKVTGNALNAAKAEDILAKRSVIVMSSGGDEPVFDEGREGHSSFTWHLMDK
                     LNKVEKFDSGADVFASVKQGLAQDGLVQTPQYGASLTAGHAVGGDYLYEVRQY"
     gene            24418..25284
                     /locus_tag="VFW59_04520"
     CDS             24418..25284
                     /locus_tag="VFW59_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363509.1"
                     /translation="MKLLQIPVLKCAAAILLSVFSLLAYAAEPVAMITDLKGKVQLNG
                     AKTHGQAAMLTYLAAGEEVVLDAGAALVLTYFDEAVEYSYKGPARILIEQKSPKVVKG
                     GIADVRQLDKEKSLGVSKFARSGKLAFATVEMRSMVIKPTLLSPVSDKISTTTPLFAW
                     KSVNEAEGYLLTLSENGGKPVQQIAVTTNSWQVPSETPLKPGMKYHWSVEETLKSGEK
                     TTVIGGFSVADAETMARVNSKRPANSASLSEKVTYALYLESEGFREDAKQVWRELAAQ
                     RPDDPNLKFRAR"
     gene            25358..>26606
                     /locus_tag="VFW59_04525"
     CDS             25358..>26606
                     /locus_tag="VFW59_04525"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX5363510.1"
                     /translation="MNQTKLKRYQWVGFALCLLLAATVHFSKISEPLDHLLLDSQFKW
                     LRQYHPQPVIHDVVVVGIDEATYAAFPEPLALWHPHLGKLFQAFAQGKPALVALDVVL
                     PERSYNFLIPQYDLPLLQGLMQLRAQSQVLLGRSVDGNGKFRKIFPPYISITGSDSQP
                     SVMVCLDADGVARRFEGNLCSDDTPVQTLAGKMAGLDGAGGKAVKGLIDYSIGAPFSY
                     IPFMDVVSWYEQGDTGKLEQMFSGKTVLVGVIMPYTDRVDFPAPIAAWEPAATKLPGV
                     LWHAQAYRSLKFHGLIHEMSKAALLILTLAAALLWFGRLGWIKAALFGVFLTGVLAYS
                     LVQLTQSLYLPVGAVLLSGTMAFIVRAIYEAVMQMRERRLLRGTFGSYVSPQILQEII
                     AGNIKPGLGGERKRLCVLFSDIRD"
BASE COUNT         5400 a         7159 c         8346 g         5701 t
ORIGIN      
        1 ggtgaggccg ggtcccagcg agtaatcgac gatgtagaaa tcgctgtcgg cgatgtcggg
       61 gtgttgcagc gcgacttcgg catcgctgaa atgacgtacc tcggctcccg attcctgcag
      121 cagacagatg agcgcatggg ataccagttc atcgtcttcc agcacgacga cgcgtttgcc
      181 ctgaaactgg ttctggctgg agatggccgg tgcaggcatc ccggccggga tggtctcggg
      241 ccggtgcggc tcgccattgc gcggcaggct cagttcgaat accgagccgc gtccggggcg
      301 cgaacggcag gtgatgctgc cgcccagcag ggacatggcg cgctgtccga tggacaagcc
      361 cagccccagc ccggcctccc ggttgcggtg cgggttggcg gcctgataga actcgtcgaa
      421 gatatgcggc aggtcggcct cgctgatgcc gacgccggta tcccatacct gcaataacac
      481 ggagtcttgt cgcggacggg cgctgatcag gatgccgccg cgcgcggtga atttgagcgc
      541 attgctgacc agattcatca gcacggattc cagtagcccg atatcggtgc ggacgatcag
      601 cgaccggttc atcgggaagc agagtttgaa acgcagctgt ttgttgaacg cggactcggc
      661 gaagttatgt tccagccagg tgaacatctc ggccagattg aacgagccta tctccggctt
      721 gatcagtccg gaatcgaatc tggagatgtc cagcaggcgt tccagttggc tggagaatat
      781 gcccatcgac tgatccagct tattgatcag ctgactttga tgaggggtcg gcgaggtgag
      841 tttaagggta tccagaaaca ggtttgcggc agcgatcggt tgccgcaggt cgtggcctgc
      901 ggcggcgagg aagcgggtct tggacagttc tttcacttcc agctggcgca aggtgttgcg
      961 cagctgttca tcttgctcga tgcgcgtggt gatgtcgctc accagcgtca tcacccgttt
     1021 ttcgccatcg agttccagca gggcgaagcg tatctctgcc gggaaggtgc tgccatcctt
     1081 gcggcgcttg ctggagatga gggtatgcgg ctggccgatt ttcagctggt gccatagcgc
     1141 gcgtcctttt ttcggatcga aatggggaga ggtgtccgcc acgttcatcc ggagcagttc
     1201 ttcgcggcta taccccaggc tgtcgcaggc ttgctggttg acctcgacga agtcgccatt
     1261 gaagtcgtgg acaaacaacg catcggcggc atgttcgatc agcgtcttgt aacgcagctc
     1321 gctttggcgg gcgcgctgtt gggcctcctt ttgcggggtc acgtcgatgc acaggccgct
     1381 catgtggggc gtttcatagt ggctggtatt gcccactgcg gtgatccatt tcgtctcgcc
     1441 gtcgggcaat acgatccggt attcgttgta cagcggcgtg tcatttgcca gcgctgcatt
     1501 gacgttttct tcggcgatct gccggtcatc cggatggatg aggttgagcc aggtggcgaa
     1561 actggcctcg gaggtcttcg gatccagtcc aaacaacaga aacagctcgt ccgaccaggc
     1621 gagtttgttg ctcgataatt cccagtccca cacgccggcc ttggctttgc gctgagccag
     1681 ggacagaagt tcattcgtgt gttgcagggc cttttccgtc tttttgcgtt cgcccagtat
     1741 cgtggtcacc atcagccccg tcagcacgat ggtgaacatg taaagccagg ccagatacat
     1801 ggcctcgtcc gcaggcatga cgccaaagac tcccttgccc agcgcggtgc cgatggcggt
     1861 gaacgcggac agcagcagca cggcgagtga ggtgccggtg atgccctgtc gtagcgcagc
     1921 ccagatgacc agcggcagcg gcaggaaggc aagcggcggg agatggtcgc gatagtcggc
     1981 gaaaaacacc agccagctga tgatgccgag caacaacagg cacagcaatg attcgacagg
     2041 ctggcgcaaa aactctttgt agctggcagg cgtgaagctc agcagcagcg gcccggccag
     2101 cagtatgccc agagcgtctc ccagccacca gttcagccag gctatcggcg cgttgttcgc
     2161 ctgcaacagg ttttccagcc acagcaggct caccccggtg gaggctgaga ccagcgaacc
     2221 caacgcggcg gcagcgatca gggcgaacag gtcgcgttgc cggttgaagg aggattcaaa
     2281 ctgccagcgc tgcagcagcc aataggtgag cagcggtgcc agcgccatgc ctgtggcgat
     2341 tccgccggca agcaggatgg gatagccaag ggagagttcg atcagcaatg cgcccagcca
     2401 gatgcccgtc atgctgctgc ttgttttcca gcgcaataag gcggcgacgg caatgccggt
     2461 aggcagccag aacagggtta tgtgatagta ggccgtggca aaggtaaagc ccaattgggc
     2521 ggtgacgaaa taggccagcg ccagcagcag cggtttgccg agcccgcgca gtgccgcgcg
     2581 gttcatgacc gtccgggaaa gggtttcacg caacatgatc aggcgaagct ggcagcacag
     2641 gtgttctccc ttcgggctgg tatcgatagc ggtggtttca ggacgtcagc gtttttccct
     2701 tgcgcaccag cgcggcgaag tcgtcggcgg ggagcggttt gctgaagtag tagccctgca
     2761 gttcgtcgca gtggttgctg cgcaggaaag cgagttgctc cggggtctcg acgccttcgg
     2821 cgaccacgcg cagtttcatg cggtgcgcca gaccgataat ggccgacgcg atctcggcat
     2881 cgtccgggtc ggtcaccacg ccgcgcacga aagactggtc gattttcagc gtgtcgatcg
     2941 ggaagcggct cagataaccg aggctggagt agccggtgcc gaagtcgtcg agcgatattt
     3001 tcaggcccag ttgcttgagc ttgtgcatgg tgtcgatggc gcgtttgggt tcctgcatca
     3061 gcatgctttc ggtcagctcg atctccagat agcgggccgc gatgccgtgc cgctcgaatg
     3121 cacgtgacag ttgctggtcg aggttgccgc tgcggaattg gcgcgcggag atgttgatcg
     3181 cgatgcagat gtcgtccagt ccctgatcca gccagaagcg aacctgccgg caactctgct
     3241 cgatgaccca gttgcccatg tccagaatca ggctggattt ttcggcgatc ggaatgaact
     3301 gggcgggcga gatccagttg ccgtcaggct gttgccagcg ggccagggct tcggcgccga
     3361 tgatgcggcc gctgcgcaga tcgaccttgg gctgatagtg cagcgtcagt tcgctctgct
     3421 gcagcgcgcg gcgcagggcg ttttccagcg tcaggttgtt gcgcgcatcg gcgttcagct
     3481 cgggcgtgta gaacgagaac tggttgcggc cattctcctt ggcgagatac atcgccgcat
     3541 cggcattctt catcagctcg gagacggact cgccatcctg cggggacagg ctgatgccga
     3601 tactgccgcc gatgaatatc tcgtgcccgc cgctgagctg gaacggttcg ctcagtgcgg
     3661 cgatcaggtc gctcgccacg acggccgcat cctgcggatc gtgcagcagg gagagcagca
     3721 ggatgaactc gtcgcccccc agtcggccca aggtgtcgcc ctcgcgctgg cgcgccccga
     3781 tgcgtttggc tacggcttgc agcaattcgt cgccgaccag atggccgaag ctgtcgttga
     3841 ccggtttgaa gtgatccaga tcgatgaata acaccccgct gagcagcgtg tgacgatgcg
     3901 cctgatccag atcctgctgc agcagggttt cgatcagcac gcgattgggc agctgggtca
     3961 gcggatcgtg atgcgccatg aattcgagtt gggcctgatt ctccttgata tgggtgatgt
     4021 cggagaacac gccgatgtag cgtaccggcg cctgatggtc gtcgtacacc gtgctgatat
     4081 tgagcagttg cggataaatc tcgccgctct tgcggcgatt ccatacttcg ccatgccagt
     4141 ggccgtattc gaggatgtcc cgccacatgg cctgatagaa ctccttgctc tcgcgcccgg
     4201 cgctcagcac gctggggttc ttgccgatga tctcttcggc actgtagccg gtgatgttga
     4261 tgtaggcatc gttgaccgcg aggatcttcg ggctggtgtc ggtgatgacg acgccctcgt
     4321 gagtgtggcg catcacggct gcggcttcac gcaactgctc ttcggtttgc ttggctgcgg
     4381 tgacatcccg tccgatgatg accagcccgg tgcggcggtt ctccctgtcg aagatcggca
     4441 ccttgcgtac ctcgaaatgg cggggctcgc cgcgctcatc ggaaacgttc tcgttgaaca
     4501 gcgtcagtcc gcccgcttgc caggccagct cgtcgttgag catgcatgcg tccagagcct
     4561 gacggcaatc gggacgcaac tcggccagct gcatgtcggt cttgccctgc cagtctatgc
     4621 catgcagctg gaacagcgtc ttggcggcat cgttggtgag ctgccagcgt ccatcgccat
     4681 ccttgaagaa gatcgcatcg ggaatggctt cgatcagcgc gctcatgcgc tcgttcaggc
     4741 tgccgagttc ggcttccgtc tgtttgcgcg tgtcgatgtt ctgtttgacg gcgatgaagt
     4801 gggtgatttc cttgccatcg aggcgcaccg gcgtgatcgt catttcttcc ggatagcgcg
     4861 tcccgtcctt gcgcctgttg atgatctcgc cgcgccatac gcggccgtcg agaatggtgt
     4921 tccagagcct ttcatagaac tgggcgccat gctcgccgga tctgaccagt tcgcggatcg
     4981 tgcgtccgac cgattcttcc agcgtgtagc ccgtcagctg ggaaaaagcc ggattggccc
     5041 actggatcac ggcctgcctg tcggcgatga cgatggcgtt gtcggccgcc gccaatgcgg
     5101 cgtcgtgcag tttgaacagg ctgcgctgct gtcccagctt gaacagttcc ttgcgttgta
     5161 tctggatggt ctcgtgcaga aaatggacat agccgccctg cagggtcttg accagatcgt
     5221 ggcgtgtcag caggccgagc agctggccct gatcggtgac gaccagatgg cggatctggt
     5281 ggctatccat ttttctgatc gcatccggca gcgtcgtgtg cggtgccacc gtgatcagcg
     5341 gtgtgtgtgc cacgccgctg accggcaggt tgccgacgtc ggcaatggtc gaggccagtt
     5401 ttaccacgtc gcgttcggtg aggatggcga aggctgcccg ctcgcgcgtg accaccacgc
     5461 aactgtagcg gttccggctc atcaggccga tcgcatcggc cagggtgtcg ttgatgtcga
     5521 cggtgacgat gttgcgcgac atgacttttt cggcgatctt gaattcagac agatcgcctg
     5581 cgtcgaggtg atgcaggaag tcgccttcgg tgacgatccc ctccaggcag ccgttctcat
     5641 cgaccacgac cagatgccgg aagccgcgct cctgcagcag gcggtaggct tcgcgaaaat
     5701 cgatattggc cgaagagcag aagggcgggg tgctcatcac ctcgttcatc ctcacgtcgc
     5761 ccaggccgcg ccgctcggcc agcaggcgca cggcatcgcg ttcggtgaag atgcccagcg
     5821 gcttgttccc cgcatccacc gcgaccacgc agctgatctt cagcgcggcc atttctttca
     5881 gcacctctga aatggtggag tcgggtgaaa cggtcttgac ggaagcggtc aggatttcac
     5941 tgagcagggc ttttttcatg gcaccgtgta ggaggcgagc tggcctcaag gatactacat
     6001 agtccgggtc tttggggcgc gctgcagtgc cgggatggac gtaaaaaagg acacccgcag
     6061 gtgtcccttt tatgactcgt gacgagccgg attattcgat cttggccttg gccttcagat
     6121 cgatgatcgc ctgctggatc gcttgctgtt gcaggcgctg ttgcagttgc gggcgcagct
     6181 cttcgaatgg aggcaccttc agatcgcgcg tatcgtccag cttgatgaca tgccatccga
     6241 actgggactc taccggctcc ttggtatatt cgcctttctt caggttctgc agggcagccg
     6301 cgaagggagg gacgaagttg cttggaacgg cccaacccag agcgccgccg gtatgagcgg
     6361 aaccggaatc catggatttt tcggccagct tctcgaactt ggccttcttg ccgagctggg
     6421 cgatgatggc ttttgcttcg tcctgggtcg caaccagaat atggcgtgcg ctgaattcct
     6481 tgttacccag cttggccttc agcttgtcat attcctgctt gagctggtct tcgctgatcg
     6541 ggtgcttctt ggtgtagtcc tgcacgaagg cgcgaaccag caccgactgt ttggatagct
     6601 cgatctcttg cacgacgtcg tcgttcttgt tcaggccgag cttggtggct tcctgcgcca
     6661 tgacttcgag attgatcagg tcttcacgga tcatcttgcg cagttccggg ctgtcgggct
     6721 ggccttgtgc tgcggcagcc ttggcgcgca tgtcgacgcg cgcttgcggg atggaggtgc
     6781 cgttgaccat ggctgcggat ttctcttcgg cgaaggctgg ctgggcagcc agcgcgccga
     6841 tgaaggccag tgctgcaaat tttccggttt tcaacattgc gagtccttat aaaaaagtag
     6901 ggttgttaaa tttcgtccgg cgttttcgcc tgtatgttga gggcgtgtat ctcgtgccgc
     6961 atcatttctc ccagcgcgga atatatcata cggtgtcgcg cgactgtgtt cttcccgaca
     7021 aactgcgccg aggtgatttt taacgcatag tgaccgccgc catcccttgc tccggcgtgc
     7081 ccggcatgct tgtggctgtc gtcgattatt tccagcgcaa cgggttccag tgcggccagc
     7141 ctcgtgcgca tggcggtgat gcggctcatg cggggaatac ttttctgaat ggggtgacgc
     7201 gtacttccgc gtacacgccg cccgccacgt agggatcggc atccgcccat tgctgtgccg
     7261 cgtgcaagga tgcgaattcg gccacgatca ggctgccggt gtaaccagcg gcgcccgggt
     7321 cggcggcatc cacggcgggg aaggggcctg ccagcaacaa gcggccctca ttctgcaagg
     7381 cctgcaggcg tgccagatgg gcgggacgcg ccgccagacg tttctccaga ctgtccggaa
     7441 catcggtccc ggtaatgaca taccacattt ataaaacctc caagaatcgt agcgagcggt
     7501 tcgagagcaa ggcgcacgga gcgcagaaac cggaatgtac ataaggtaca tgaggatttc
     7561 gagcaccgcg caacgcagct atcgaatcgc gcagtagatt gttggaggtt ttcatcagtt
     7621 gttttccttc ttctcgtcca catatttcga cagccaggtg ctctgtccca gaatgaacac
     7681 caccatcagc gcggtgaagc cgaacagctt gaagttcacc cagatgtcgg tggaatagtt
     7741 gaatgcgata tacaggttga gcatgcctaa cgccgcgaag aacaggctcc agatcaggtt
     7801 gaggcgttgc cacacatgca ggggcagggc gaccttctcc tgcagcagcg agcggatcag
     7861 gttcttcttg aacagcaggt tggagaacag cagcacgacc gagaacagcc agtacagcac
     7921 cgtcggtttc cacttgatga aactttcgtc gtgcaggaac agcgtcgcgc cgccgaacag
     7981 ggtgatgatg gcgaagctca cccacagcat ggcgtcgatc ttgccgtgac ggtattttga
     8041 ccagatcagc tgggcgaggg tggcgaccat cgccacggcg gtcgcggtat agatgttgaa
     8101 cgccttgaag gcgatgaaga acaggatgac ggggaacaga tcgaacagta gtttcatggg
     8161 agtgtgctgc gtttaaaaga ggttatttgc ggtccagcgc gagggcggcg gagttgatgc
     8221 agaagcgcag tccgctgggg ggcgggccgt ccgggaaaat gtggcctaga tgggcgtcgc
     8281 attgtttgca gcgcacctcg atgcgctcca tgccgtgcgt gttgtcggcc agtgcgtaga
     8341 cattgtccgc gctgtacggc tgggtgaagc tgggccagcc gcaaccggat tcgaattttg
     8401 cttccgaatc gaacagcgca ttgccgcagc acacgcaacg gtagaggccc tcgtcgtggc
     8461 agtcccagta ggcgccgctg aaggccgctt cggtgccgcg ttcgcggcat atcctatatt
     8521 gctcgggcgt gagttcgtcg cgccattcgt cgtcggtctt ggtgattttg ctcatggtca
     8581 tgtgttcagt gtgctgcgta cgctgggacg ggcggctatg cgctcgaacc aggctgacag
     8641 gttgggatgg ctgccgcgcc agtcgcggtc gggctggcgc agatcgagat agatcagcgt
     8701 gctgaccagt gccagatcgg ccagtgtgag ggcgtcacct tcgcaccatt cgcgttcgcc
     8761 cagcagatcg gcggcatagg ccagtgcgcg ggtgatggcg gtgtaatgca gggtgtgcac
     8821 attggcatcc tgcttctccg gtgcgcgcag actctcggtg cgcaccacca gcgccgaatc
     8881 cagaatgccg tccgccagcc cttcccagcg ccgcacgcgc atgcgcgcca gagcatcatt
     8941 gcgcgggatc aggatgggat ggtcgttcag cgtgtccaga tattcggcga tcaccggtga
     9001 atcgaacacg cagctatcgt cgtccagtat cagcgccgga atgcgcccca gcggattgat
     9061 ctgtgccaca tggctgcccg gatcgcgcgg ggcgtcgacc agatactcgt ggggaatgtt
     9121 cttctccagc agtaccagtc gcaccttacg cacgtagggg ctggtgtcgg tgccaagtaa
     9181 tttcatttgc ttaaattgaa tagttcgtta gtgaaattcg caatttcgat ggggcctcgg
     9241 ggtggtggga gcggattctg ccgcgattgc cgtgaagcag ttggctgaca tatcgcggct
     9301 gaagccgctc ccacgatccg tgctcctaca gcacctcgcc tgcgtagtcc gccagacgcg
     9361 aacgctcgcc gcgcatcagc gtgacatggc cgccgtgctg ccagcccttg aatcggtcca
     9421 ccacataggt caggccggag ctgccttcgg tcaggtaggg cgtgtcgatc tgtgcaatgt
     9481 tgcccatgca caccaccttg gtgccgggac cggcgcgggt gaccagcgtc ttcatctgct
     9541 tcggcgtgag gttctgcgcc tcgtcgatga tcaggtactt gttcaggaaa gtgcggccgc
     9601 gcatgaagtt cagcgacttg accttgacgc gtgaacggat caggtcgcgg gtcgcttcgc
     9661 gtccccagtc gccggcttcc tcgctgttct tgttcagcac atccatgttg tcttccagcg
     9721 cgcccatcca aggcgtcatc ttttcttcct cggtgcccgg caaaaagccg atgtcctcgc
     9781 ccaccggcac ggtcacgcgg gtgatgatga tctcggtgta gagcttggtc tccatcgtct
     9841 gcatcagccc tgccgccagc gtcagcagcg tcttgccggt accggcctga cccagcagcg
     9901 tgacgaaatc gatctccgga tccatcagca ggttgagcac gaaattctgt tcgcggttgc
     9961 gagcggtgat gccccagatg ccattcttgc tgtgggtgta gtcggtcagc gtgcgcagca
    10021 gcgcgctctg atcgtcctgt tccaccacga tcgcgtagaa cggtgtggcg ccgttagtct
    10081 cctgaaagac gaactggttg acgaacatcg cgccgcacag cgggccgcgt atctggtagt
    10141 aggtatgggt ctctttcacc caggacttca tgtcccggcc gtggctctcc cagaagtcgt
    10201 ccggcaattg cagcacgccg ctatacagca ggtcggtgtc ttccagcacc ttgtcgttga
    10261 agtagtcctg cgcttccagg cccaacgcgc gcgccttgat gcgcatgttg atgtccttgg
    10321 agaccaggat caccgagcgg ttcggatgtt cctgctgcat gccgatgacc acgcccagga
    10381 tggcgttgtc cgccttgccc agctccatgt tttgcggcag ctcgtgcttg atgaagctgg
    10441 tctgcaggaa caggcggccg gtgcagttct tgtgcgcctg agattccagc gcgatgccgg
    10501 cctcgatgtt gtgcggcgca tcgctgacga tctcgtcgag gaagcggctg gcctggcgcg
    10561 cgttgcgggc gacctcggtc atgcctttct tcttgttgtc cagctcctcc agcacgaaca
    10621 tcggcaggca gatatcgtgt tcctcgaagc ggaacaggct ggtcgggtca tgcagcaaca
    10681 cgttggtgtc gagcacgaac agttttgttt cgccgctgac tttttgtacc gtgctgactt
    10741 cttttctgac cgttcttctg cgcgtattgg taggcatgac gttcctttag tcgagagagt
    10801 taacgaaatc cagtacttcc tgtgcatgac cgtcggcctt gagcttgcgc cattcgcgac
    10861 gcagctcgcc gttcttgtcg aatacgaagg tgctgcgctc gatgccgcgc acctgtttgc
    10921 catacatcat cttctgtttg atcacgccga acagttcgca tgcgatttct tcctcatccg
    10981 agagcagctc gaagggaagg gtgaactttg ccttgaagtt ctcgtgtgac ttgatgctgt
    11041 cgcgcgatat gcctaccacc tcgcagttcg ccctagcgaa tgccgcatgc aggtcgcgga
    11101 actggatcgc ctcggtggtg caacccgggg tgttgtcctt ggggtagaag tagatcacca
    11161 gatgcttgcc gcgtgcggcg gagagggtaa aagtctgacc gccggtggcg gggagggaga
    11221 aatcggcgat gctggactgt ggcatggatg gactcggtga acgatggctg tattcttgtc
    11281 aaaaaatcgc ttaaaggcaa gcagcgttat cgagatgcgg ctaagccctt gtctattcag
    11341 ggggtgatgc gttggagaga ctcagaaggt atgccattac ccatggggct aacccgccgc
    11401 gccgaggtgc cggatagtcg cccccctgaa aaacgcctcg atttttccac ttgcttagta
    11461 gggtttctgg ctgctagatt gcagctatgt tttttcggtg cggtgtttga gaacatgata
    11521 tctggcctgt cgggcggtca tgaggtcggc gcgagcgggt tgcgatgccg acggaccggc
    11581 tcatcggttc tcaacccggt gaaaatccaa cccaatcgct tttaattcga gaatgtgccc
    11641 atttctgcat ccgcgcgtat cgcatggctt tgtcctcgtg gcattgctct cgctttcgtg
    11701 cggcttgcgt gctgaggtac tggttgtggt cgaccggaat agtccgttga ccgcgatcga
    11761 caggaatcag gtgtgcgaca tctttctcgg caaagtggct gcgcttcccg atggccgcat
    11821 ctcgacgctc atcgatcagc ccgacgcgag tccacttcgc gaagagttct acatgaaggt
    11881 cgccaacaag actgcggccc agaccagggc gcactgggca aagctttact ttaccggtcg
    11941 cggggtgccg ccccggcagg cgcaaaatag tgccgaggtc atcaaaatga ttcactcgaa
    12001 gcccggtgcg attggctatg tcgagcggtc ctcgctcgac tccagcgtca aggttattta
    12061 tgtcgcaccc tagtcaggaa ttgagcggag acgcaatcag atgaacgatc ctttgctgaa
    12121 aatactgatg ctgatgttcg tgctgctgac aacccgcagt catgcggacg aaggctcggg
    12181 cctcgaattt tcaggctccg gttttcttac cgcgagtgtc ggaaaaatga tgggcggcac
    12241 aacgggcaat gtcggcgact ataactgccc atgtttcgtc tcggactatg ctcaggctgg
    12301 aatctttgac ggtcgtcgca gcctgcaatg ggggcctgat tccaagttgg ggttgcaagg
    12361 gcaggtgtca tttgacaacc agaagttttc ggtaacgggg caggttgtcg cccgtggcgc
    12421 aagcaatggt cgtgcagatc tggaatggct ctatggcacg tacaagctga acgacaaggt
    12481 ggttctgcag gctggtcgca agcggctgcc gatgttctat ttttccgatg cgcaggacat
    12541 cggtgtcgcc ctcccctgga ctcatttgcc gacatggtta tacggctggc aagcggtgaa
    12601 ctacaacggt atcaatctcg gctatcggga tcaattcggc gactgggcgg ccagtgccaa
    12661 cttgctcgcg ggagacgagc acaggaagaa tagcggctac tggaaagtct atggcaacgg
    12721 tcggcaaagc gtgaccgatg tgaactggac gaatatcgcc ggtggcgatt tgaccttgtc
    12781 cagggattgg cttgaaacac gcgtcgtgta tttgcaatcc gacactcagg atgtcaatgt
    12841 caatggcacc tggaactacg cgagcctgaa ctacgatccc ccgaccggaa tctcgcccat
    12901 cgccaaacaa aagatatacg gtttaaccgt caaagcggat tacgagaact ggctgttctt
    12961 caacgagttc atttatatcg accatcccgg tttgacctac agggattttg cccagatcgt
    13021 ctccgtcggt taccggcaag ggaagtggat gccgatggct acatgggggc attaccgggg
    13081 tgccgtggtc tcgcagggcg tgttgccagg ggcgccggca agtgtcgcca acagccagca
    13141 gaccgttacg ttgagcctgc gttacgattt gacgactaca agtgatctca agttgcagtt
    13201 cgacgatacc agcgacaaca gtgagcccgg attcaacccc cgatacggga gctcgcgcct
    13261 gctgacgctg gcttacgatt tgatgttctg atcgccatga cggttctgcg ctggatcatc
    13321 cggtatcgaa gaagctatct ggcgttctct gcgctgctgg tgctgttggt cgtctctgtc
    13381 gttgtctggc ggagcaatct tgatcttgag aaaaagcgcg agctgccgtt ggccaggagt
    13441 ctcgcgacat tggcatccac gatagagagc ggcacgataa acagtcaggc gatgggggcg
    13501 gcgatcctgc tggggctgga gcatcgtgag gtcaaacagt tggctgcggg ggcgttgcca
    13561 ccggatgcgc ccattgtgtt gtccggactg gatgcgatac gcgaacagtt cactgccaat
    13621 gccgtttttc tggtcaacga gcagggattg gtcgtcgcgg acagtcgtga aggcggtcgg
    13681 agtacgacgg ggctcgacat gtcgtcatgg ccctgtattc aactggcaat acagggaacc
    13741 cccaatgttt acccggtgat gggcggcgca gaaagcgacc ggggcatcta cctcgccgca
    13801 cccttgcgcg cctccggaag tcgcgcttcc agagcgatcg gtgccgtcgt ggtcagggtc
    13861 ggtgcggaca agctggatat gctgctggag agctggacgg atggcatcgc tttgttgtct
    13921 tccccgcagc gagaggtctt ttccgcgaac cgtctggatt ggctgttcgg caggacaacg
    13981 gtcaatcggg ttgccgatgc ccatcgcgcc ggcagagctg gcgttgcgca aaccggctac
    14041 cctcgactgt cattttcgct ggaggctccg gaaaccacga ttgaaggtgt gcggtatgcg
    14101 gtgcgcagcc agttgctgga gtggaacgat ccagcgggag actggaggct ggtactgctg
    14161 gggagaaccg ttccctggtg gacggatgga agggtgctga gtcttgccgg attggcaggc
    14221 ctgcttgtcg cgttgctatt gttctggttt tacaccctcg ctcgccatga aaacgagctg
    14281 ctcaagagtg atgcgctgct caaggagtcc cagaatatcg cggccttggg gagttatgtt
    14341 ctggatactc aaaccggggt attcgaggtc tccggcgaga cggatgtctt gttcgggata
    14401 gacgggacct atgaccattc tgtggcggga tggctggcgc tgatccatcc ggacgaccgc
    14461 gcgatgatgg agcgttactt caacgatcaa atcactgggc agagtccatc cttcgacagg
    14521 gaatatcgca tcctccgcta caacgaccag caagagcgct gggtgcgtgg actgggccgg
    14581 atgatattcg actctgcagg gcggcctctg aaaatgcacg gcacgatcca ggatgtgact
    14641 gaccgcaagc aggccgagca ggtgctcaat gaatcgatac gccaactgaa ggaaaaagag
    14701 ttggccaaga cacgcttcct cgccgctgca gggcacgatc tgaggcagcc gttggctgcc
    14761 gccaacctgt tcatcgatgc ccttaaattt accgggccaa cgcccgacca gaataagatc
    14821 atccagaggc tggatcaggc aatggggact ttcaatggat tgctggaggc tttgttgaat
    14881 atctcgaagc tggatgcggg tgtcatcaag cccgaataca ccccgatcca ggtgcccaag
    14941 ctcctcgact ggctggagca gagtttttca gcgatggctc acgagaaggg actgggtttc
    15001 aagacgcgct cttcgatgaa ggaggcgctg gtcatttgca gcgatctgga tttgctcaag
    15061 tcggtgttga tgaatctggt ctccaatgcg atcaagttca ccacatccgg tgcgattctg
    15121 gtgagcgcca gacgacgggg ggaggaggtg ttgtttcagg tatgggatac cggtataggc
    15181 atcgatcccg aacatctcga aaatatcttc gatgagttct atcagatcaa taacccgcag
    15241 cgcgacagga cgagcgggct ggggctgggg ctggctatcg tcaatcgcgc attgatgctg
    15301 ttgggcgtgg aggtgacctg ccgctcgaag atcgggatag gatcggtctt cgggtttcgt
    15361 ttgccactgg cgcgcgcctc ggatatggaa acgcatcaga gcgccacggt gacttcccgg
    15421 aatgaggcga ttgacgagct gttcgcacga ggaaaacgat tcatcgtggt ggaagacgac
    15481 cggctggtcg cgcaggccat ggtcaattgt ctggaggcaa tgggcggtga ggtggtctat
    15541 ttcaagaatg cggaagaggc tttgcagcac gccaacatcg agttcggcga ttattacatc
    15601 gccgattaca tgctgggcgg caggttaaac ggtatccagt tattgaatca gctccgtcaa
    15661 cgcatgggca ggcacatcaa ggcggtgctg ctgacgggcg atacctctcc cgcctttatc
    15721 cgggaggctg aggattgcaa ttggccggtg ttgcataagc cggtcaatac ctccaggttg
    15781 atttccagtc tcagcgcgca gcaggaaagc gcgcgttgac cggttgcgaa aggccgatgg
    15841 atcaatgccg acgcgatcgg tgtatgccgg gtaacatgtc ggctcaattg agataagatc
    15901 gcacgcagtg gttggtcatc ggatggagaa gcgtgcattt aactgaaaag gagtcatcac
    15961 aaatgaatga agccgtgaac gaacccgtaa aagttgtgca gtttattatt cgagagggcg
    16021 atgtggccga tttgtgtctt gaccagaacg ggaatgttta caaggtcgag tatagcggca
    16081 ggaacgagct cactttaatc aagcaaacga tactggttgt gcatccctga tcttgccgca
    16141 acggcacggt gtccctggat gcgatcgacg tggccgcggt gccggactat aggttcatca
    16201 gctgcaatac gcgcgcgttc gacagggaca gcttttcgtt cagcgacctc aggaaggcgc
    16261 gatccatacg gatgcagacg tctttcgaaa agccttccag ttgcttgcgg gaaacttcca
    16321 gtgcgatgac atccccctgt gccgacacgg tcgcacccct gatcgggctg ccattgtata
    16381 aacaggcatt ctccccgacg cagtcgcctg cctgaatgat gttgagcata cgctgccgtt
    16441 tgcttacgac gacactccct tgcagcagga tataaaaaga gaatccatgt tcgccatcct
    16501 tgatgatctc ttcgttatgt ttgaaggtgc gccattggga ggcgtgcagc acttcccata
    16561 tttccacggt caggaaatcc ttgaaaaacg cactgcgccc catcagctcg aacatggtct
    16621 ggtcatcgac caggctttcg acccgattca ctcccgaggc cgcatataag tcgctcagga
    16681 actccttcca gtcttgatag cggtcttcaa ggtttttctg cagggcgcga tccacgatgc
    16741 ggtttatttc ttgcggcagt ccaacgcgat ggatctccat cggaatgtag gggtgattga
    16801 ggatctggtt gatggccgca tagtgatcgg ccgctttgaa ggtgttgtgc ccggtcagca
    16861 ggcgatacat caccgcgccc attgagtaaa tgtcggactg gtgattcagc gccgtccccc
    16921 tgatctgctc gggcgacatg tagctcaaac ttcccgctcc cgttatttgc gtggtctccg
    16981 aatcgaacag caaggcacag ccgaagtcgc tcagtttgac taatccgtcc actcttagaa
    17041 tgatgttggc cggcttgacg tcgcgatgta tcacccctcg ctggaaggca taattcaagg
    17101 cctcggcagc ctgcctgacg atgtccatga ccgtttcgac ggcgagcagt ttgtcgggag
    17161 tcgaaaagtt ctctaatgac tgcccttcca catattccat gatgatgtaa cttcccgtca
    17221 catcgttgac gttggcctct atgagcgaaa cgatgttggg gtgtttcagc ttcccgcaca
    17281 gggaagcttc gacctcgaac atcttgtgct ggatgccgat gctgcactcg gggcgtattt
    17341 tcttgatggc gaccagtttt aaggaggtcg tatcgacgcc cagatagacc gtgctggtcg
    17401 cgccgtgacc taatacccgg gtgatcctgt aattgtcgat atcctcaatc aagggggaat
    17461 gaggctcatt cagcttgtcg gtcttgtcta gattggaatt gggcatgttt tttcagtgat
    17521 ccggggccgc attcgggagg gtggatgttt cagatgccgt aacgggacag cgcttgttcc
    17581 gattaaacag aagtgtctct aatccggccg cctggttcct gtcaataggt ctacaggcct
    17641 attgaatttt tcctacatcc ctgtacattg atgatggtga taatacccga gtatgcggca
    17701 aataaaatca aggagggagc ggggatggtt aagcagttgg tggtggtttc tggacttttc
    17761 ttgcagctac tgatggcctg tccggcattc gctgagtcga taaaatttga agtgacaggt
    17821 ttctctgtcg aaggtaatac cttgctgccg gccgagcagg ttcaaagcgc gctggctccc
    17881 tttgtcggtg ccgatcgcga aatggccgat gtgaacaagg ctgccgaggc attgcacgcg
    17941 ctgtatcaga acgcaggcta cgcggtagtg caggtgatcc caccgcagca aatcatcacc
    18001 agcggcaatg tcctgctgaa agtcgtcgaa gacaagatcg tttccatcga ggtgacgggt
    18061 aacaaggcat acagtgcgga gaatattcgc gccagcctgc ccgcgttgaa gccgggcgcg
    18121 agcctgaatg cgaagcgtct tgaatcgggc atcgtgctcg cgaacgagaa ctcggccaag
    18181 caagttgcgg tgaatgttca gccgggtgcc aagttgggtg acatcaatgc gcgcatcgat
    18241 gtcaccgagg atcgtattga aaaatacatc ggcacgatag ataacaccgg ctcggctgcg
    18301 accggtttta ccaagatcgg tttcgcctat cagaacgcca atctgttcga tcgcgatcac
    18361 gccttgacgc tgcaatacag cggctcgctg gacatgatcg acaaggtgta tagcctgagt
    18421 gcgggttatc acgttcctct ctatagccat aactcgtcgg tcgatctgat cgcggcctat
    18481 tcgtcctcct cgacgaccac cccgagcggg gctggcagca tctacttttc cggccaaggc
    18541 tcgattttag gcgcccgtct caattacgcg ttgcccagcg tcggcgagat acagcacaag
    18601 gttattctgg gtgcggacta caaggacacc accaaccagt tcagcggttg cgttgcaccc
    18661 tgcggcagca tcaccgaaat tccgctcagc ctgacctatt tcgcgcaggc gaataatccc
    18721 ggttttcagg ggagcggcaa tgtttcctgg ttgcaaaaca tcagcggggg gccgcatggc
    18781 ggcttggccg actatcaggc atcgcgcttc gtttatcctg gcatctcggc caagccgacc
    18841 tggagtctgt tgcgtttcaa tgccaccggg gccgtgccgg ttgcgcaaga ctggcaggca
    18901 cgcgcgcagc tcaatgggca gtacaccaat gaaatgctga tcggcgccga gcagctcggc
    18961 gcaggtggcg cgtcctcggt gcgcggttat ccggagcgtg tcgctgcggg tgactacgga
    19021 tatgtggtca atctggagct ctacacgccg gatatgaaca agctcgtcaa tctgagtaac
    19081 ggcagcttgc gggccttggt gttctgggat gcggccagcc tgacggtgaa cgaccagtgg
    19141 ggcccggcga tatcgcgcaa cactaatttc gatagcgtcg gtgtgggcgc acgcctgatg
    19201 tacaaaaaag atctcagtat caaactcgac gtcggctggg cgcaaaaagc gctgcccggt
    19261 gcggcgccca gtccggtcaa acagaatgat gttgccggtc atgtcgccgt tgcctacata
    19321 ttttgaattt agctcaccga aaatctggaa cctaaatcat gagagcctcc aagaaatccc
    19381 tccccgtatt ccgtttgaaa cttgtcgcgc tggctatcgc cgcgtgtttt tcgccgtgcg
    19441 cctttgccaa tccggtcggg cctagcgtcg tcaatggggc ggccagtttt gccgttaacg
    19501 gcaataccct gaccgtgacc aacaccccgg gcgcgattgt gaattggcaa cagttcaaca
    19561 tcggcgccgg acagaccacg caattcatcc agcagtcggc acaatcctcg gtgctcaatc
    19621 gcgtgatagg tccggatccg agtcagatcc tcggtatcct gcaatccaac ggtcgggtct
    19681 acctgatcaa tccgagcggt attctgttcg gtgcgggcgc ggtggtggac gtgggcggcc
    19741 tgatcgcctc cacgctcaac atcaccaatg ccgatttcct gatgaaccgg atgagcttca
    19801 acggcaacag cggtacctcc gtcgttaacc aaggcacgat caccacgccg tttggcggca
    19861 gcgtgtattt gatcggcaac aacgtcagca acgaaggggt gatcaccacg cctcaggggg
    19921 cggtcgtgct ggccgccggc aacagtgtca gcatggtgaa tacggtcacg ccgcacgtca
    19981 gtgtcaccgt gagtgcgcca gcgggcggac aggcggtgaa tctggggcag gtcacggcgc
    20041 aaggcggctc catcaatatc tacgcgtcgt tgatcaaaca gcaaggcttg ttgagtgcgg
    20101 atagcgccag cgtcaatgcg caagggcaga tcgtgctgtc ggcatcgcag acggtggagg
    20161 tggcggcagg cagcagcact tcggccgcca acagcaatgg cgtgggtgga acggtgcaga
    20221 tcctgggcaa tcaggtcaac gtcgcgggtg ccgtcgatgc gtccggtagc gcgggaggcg
    20281 gcaccattct ggtcggcggt gattttcagg gcaagaatgc ggccgtgcag aacgcggcca
    20341 acacgacagt cgcttcgact gcgaccctca aggccgatgc gctcgtgaat ggcaatggcg
    20401 gcaaggtggt ggtgtgggca gatggcacca cgtcctataa cggcgccatt acggcaaaag
    20461 gcggggcgct gggcggcaat ggcggcaacg tcgaagtgtc gggcaagggc gtgctggatt
    20521 tcaagggtac ggtgaatacg ctggcgccga atggcacggg cggctcgctg ttgctggatc
    20581 cctttgcgtt atgcatttcc tccacagcct gtgcttattg ggatgtggtg accgggacct
    20641 atacctctcc ttccagctcc atgactgggc tgacattgaa agcgctggtg gcggccaatg
    20701 gcgcgaccac ggtacggaca tccggttatg gcgggggaca cctgttggtg tctgattcca
    20761 tcgtttttac cccgggggag ttatccggtg gcaagacgct cacgctgcag gcggataacg
    20821 gagagctggc attcaggaac actacgatcg atgcgacggg cggcagtggc gcgctgaatc
    20881 tgaacttgat ctccacgggg catccggcct atggcggctg gattggaata gatagttcga
    20941 cgatcatgac caatggcgga aatatctata tcaacgcgcc aggcggtgtg tccattggaa
    21001 cgggttatgt taacgggcca accgctgcat caacggtcgc cacggcaggc ggcaacatca
    21061 gcatcgtcgc ggcaaatgtg gaggtgggga gtccctggag cacgggcacg aatatcagca
    21121 cagggaatgg caatgtctcg attacgacca atgcggtttt tggcggcggg aattcggcat
    21181 cgatcctggc gggaaagtcc atggtggttt atggcggcac ggcggtcaac tggggaaccg
    21241 gcagtctggt ggtgaacggg gtgaccgacc cgatatcttc tggcgcaggg gcgcttacca
    21301 tcccctccaa tcagtattgg ggcgccaaca cgaatggtgc cttgccgttg agcagcttcg
    21361 cgggcaattt ctcgggaacg tcaatcgtcg atcctatggc gggtggcgga accatgacct
    21421 ggtcgatcac cgtgaacgca acggggcaag tgacgatgac gggttcgggg ccgaatacca
    21481 ataatcaagg gatcggtcag ctttacccct tgagcaacgg caccttcatg atccacttcc
    21541 ccggcggtct gacggtgtgg ggatcggtcg atccggcaac ggggacgctt accggtatct
    21601 ggggcgttaa tgggaacacg ctagggcagc agggctctgt gcctcaaggg atgaactggg
    21661 gtacgtttac ttcggttgcc ggagggggca tgttgcagca gctgtgcacg ttgaatccgg
    21721 ccctgtgcag tcccaccgtc attacgccgc cgacgccgcc ggtgaatccc ggcaccagca
    21781 tactggataa caatacggcg attgccgatc ttaccttcag tggcgggctg aacgccgagg
    21841 gcgggacgat gtggatcacc tacgacgatg tgcgcgaatc cagagatgag gctagaaagg
    21901 ccgagcgtga aaaggacgca gccgaggagc gcgtaaaaac cgccaagacg cccaaagaaa
    21961 aagaagacgc gaaaaaacat ctggcctcca aaaccgctga ggtggaattg aagaaggcgg
    22021 atgcgagcat ccgcgaaacc gagtccgagg tgcgtgcggc gaatgaagaa gtgagagcag
    22081 ccaagacgcc ggaagacagg atgagggcgg aaaatcggaa atccgtggcc gaatccaagc
    22141 gtgcggaagc cgaagtgaag aaggccgatg cagaggttaa acaagccgag gctgaggcca
    22201 agcatgcaga ggggcctgcg gccagggctg cggccgaatc gaagaaacaa gcggccgaga
    22261 gcaagcgtgc agaggccgag gtgaagaagg ccgaggtgga tgcgaagaag gccgagaccg
    22321 agctgaagca ggttgaggct gcagcgggtg ccaatgaggt gcctcaagcc gaggtggaga
    22381 ccaagaaggc cgaagtggcg gtcatgaagg ccgaggccga gatgaaacag gcagaagccg
    22441 aagctaaaac ggcaaaagat cctgaagcga aagcgttggc cgagaccaaa ttggcggccg
    22501 ccgaagtcaa gaaggcccaa gccgaagcca aacaagccga agccgaggta aagcaggaag
    22561 aagccaagac gggtgacgca ggagtacctg ccgatcaacg caaggtcgag gcaaaaaagg
    22621 ccgaggctga agtcaaacag gctgaagcgg aaaccaagca ggccaagctg gaagagaagt
    22681 ctgccaagga tccggaagcc aagtccaaga gcgaagagac ggtagccaag gcggaagcca
    22741 agcaagccga ggctgaggct aaagctgcga aaatcaaggc agatgaggct aaagactcac
    22801 ccgaagcccg tcacgaagcc gaagccaagc aggcgaatgc cgaagccaag aaggccgagg
    22861 tagaagcgaa gaccgcgcgt gatgatggtg gccgttcgca ggccgaagcc aagcgtgccg
    22921 cagccgaggc caaacgcgcc gagtctgagg agcaagttgc gcgtgaagaa gtcaagcgtg
    22981 caagagacga ggaaagggaa gccaaaacgc cggagagcaa atacgtcgcc tcgaagaaaa
    23041 tcgaggcgag cgaagccagg gcagccgcca aacgcgaaga agcggcggtg aaaagggccg
    23101 agtccgaaat gaagcgggtg gaagccgaag tcaagcaggc cgagatcgat gtcaaagaag
    23161 cggcaacgcc tgagcagcgc gcctctgccg agaaacgcat cgctgaaaag aggaatgagt
    23221 tcgctaccaa gcgtggtgag gttgcagaaa aatcacgcag ggccgagtcg gtgaaggaac
    23281 tcgcggaaag caaggagtcc gagttcaagg agaccaagca gcgccgggat gatatagcgg
    23341 agactgcctt tgccggtatc gatgtgaagg cgatgagcaa agatcgcgtt caggatgcga
    23401 tggctgcccg tcacgagttc atgaaggata ccttggcccc ggccctgacg cttttgaagc
    23461 gtgacccgaa tgccggcatc aaggcctgtg tcgagggggg cggtgaagtc tgcatcaagt
    23521 cttcttcgca ggtgcctgcc tatattgcga atcctgccgt tgaagtgaga ccgcgcgtgc
    23581 agctgcctcc gcttgccgtg ccggcggtct cgttcctgcc gcaaattcag cgcaagattg
    23641 ccgtggtggt gggtaacaac gcctatcagg atccgaattt accttcgttg aatggcgcga
    23701 tcagggatgc cgatgcgatt gctaacgtat tccaggaaaa gatgggctat gaagtgcggg
    23761 tcgttcataa cggaacgcgt gccgacatca ttagcgcgct caacaaggtt gccgatgaag
    23821 tcggttccaa cgatagcgtg gtgctgtatt acgccggaca tggctatcag gacgatgcga
    23881 ccaaggtcgg atactggatt cctagcgatg cctctacgcg caatgcggac aactggatta
    23941 ccagttcgga aatcaacaag atgctgacga acattccggc taaacagttg atgctggtat
    24001 cggatagctg ctactccgga gcgttgacca atgaacaaaa agtcaccggc aatgctttga
    24061 acgcggcgaa ggcggaggat atcctggcaa aacgttcggt gatcgtcatg tcttcaggcg
    24121 gggatgagcc ggtcttcgat gaaggtcgcg aaggtcactc ctcgtttacc tggcatctga
    24181 tggataagct gaacaaagtc gaaaagtttg atagcggagc ggatgtcttc gcttccgtga
    24241 agcaaggttt ggctcaggat ggcctcgtgc aaacaccgca gtacggcgca tccttgacgg
    24301 ccggacatgc ggtaggcggc gactatctgt acgaagtgcg gcagtactga tgatcgatgg
    24361 ttcgcggatc cggggcgggt tcgcgtgagt gcagtggttc acattgagga gatggacgtg
    24421 aaacttttac agattccggt tttgaaatgt gcggcggcca tattgctgtc ggtgttttcg
    24481 ctgttggctt atgcggctga gccggtggcc atgatcacgg acctcaaggg caaggttcag
    24541 ctgaacggtg cgaagaccca tgggcaggcc gccatgctga cgtatctggc tgccggggaa
    24601 gaggtcgtgc tggatgcggg ggccgcgctg gtgctgacct atttcgatga ggcagtcgaa
    24661 tattcctata aaggccccgc gcgcatcctg atcgaacaga agtcgcccaa agtcgttaaa
    24721 gggggaatcg ccgatgtgcg tcaattggac aaggagaaat cgctgggggt gagcaagttc
    24781 gcccgcagcg ggaagctcgc ctttgcgacg gtggaaatgc gttcgatggt gatcaagccg
    24841 acattgttgt cgccggtgag cgataaaatc agcacgacga cgccgctgtt cgcatggaag
    24901 agcgttaacg aggcagaagg ctatctgctc acgctgagcg aaaacggagg gaagcctgtg
    24961 caacagattg ccgtgacgac caattcctgg caggtgccga gcgagactcc gctcaagcct
    25021 ggcatgaagt atcactggag cgtggaggag accctcaagt ccggtgagaa gacgacggtc
    25081 atcggtggtt ttagtgtggc cgatgcagaa accatggcca gagtgaattc caaacgccct
    25141 gcgaattcgg ctagtctgtc tgaaaaagta acttatgcgc tctatcttga atcggaaggg
    25201 ttccgcgaag atgccaagca ggtatggcgc gaacttgccg cacagcgccc ggatgatccc
    25261 aacctgaagt tccgtgcacg ataagattcc ggggggagtt cggataacct gatttccccc
    25321 ggacactccg gcctccgata aagatcaagg actcctcttg aatcaaacga agttaaagcg
    25381 ataccaatgg gtaggcttcg cgctgtgtct gttattggcg gccaccgtgc atttctcgaa
    25441 aataagcgaa cccctcgacc atctcctgct ggatagccaa tttaaatggc tacgtcaata
    25501 tcatccccag cccgtgatcc atgatgtggt ggtggtcggg atcgatgagg cgacctatgc
    25561 cgcctttcct gaacctctgg ctttgtggca tccgcatctg gggaagttgt tccaggcttt
    25621 tgcgcagggt aaaccggcct tggtagcact cgacgtcgtg ttgccggagc gctcgtataa
    25681 ttttctgatc cctcaatacg atctgcccct gcttcagggc ttgatgcaat tgagggcgca
    25741 aagccaggta ttgctggggc gctcggttga tggaaatggc aagttcagaa agatatttcc
    25801 cccctatatc tcgattacgg gatcggactc ccaaccttcg gtgatggttt gtctcgatgc
    25861 cgatggggtt gctcgtcgat ttgaggggaa tctctgttcg gacgatacgc cggtacagac
    25921 tctggccgga aaaatggcgg gactggatgg ggcaggcggg aaggccgtca agggattgat
    25981 cgactacagc atcggtgcac ccttttcgta tattccattt atggatgtgg tgtcgtggta
    26041 cgaacaaggc gacaccggca aactcgaaca gatgttttcg ggcaagacgg tgttagtcgg
    26101 cgtcatcatg ccttataccg acagggtgga tttccccgcg cccatcgcgg catgggagcc
    26161 cgcagcgacc aagcttccgg gagtcttgtg gcatgcacag gcctatcgtt ccctgaaatt
    26221 tcacggcctg attcatgaga tgtcgaaagc ggcgctattg atccttacac tggctgccgc
    26281 cttgctatgg ttcggccggc tcggctggat caaggccgcg ctgttcgggg tgttcttaac
    26341 gggcgtactg gcgtactcgc tagtgcagtt gacgcagagt ctttatctgc ctgtcggtgc
    26401 ggttttgtta tcgggtacga tggcattcat cgttcgggcg atatacgaag cggtcatgca
    26461 gatgcgcgag cgacgcttgt tgcgcgggac tttcggcagt tatgtgagcc cgcagattct
    26521 gcaggaaatc atcgcgggca acatcaaacc cggcttgggc ggcgagcgca aaagattatg
    26581 cgtattgttc tccgacattc gagact
//
LOCUS       DASUIV010000119         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_586523, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000119 DASUIV010000000
VERSION     DASUIV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586523"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VFW59_04530"
     CDS             <1..867
                     /locus_tag="VFW59_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5363511.1"
                     /translation="YLRTMVDEIVTPVAHIQGRIRALDERVCGVADNAVNEQGHIQTI
                     ASTMEEFSESVAEVAHMAADSLNDAKSMQQTVEENNRNMELSIEATSRVADTVQSSSR
                     TISDLGASIEKIGTIALAIKEIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLA
                     ERTAASTKDIASTIGEINTISDAAVHAMNGAVSEVETGIALIRKNGEGLKEIMSATVN
                     VANRIEHIATASNEQSTAGESVVSSLERISGLVDSNTHAAKDAKAEAESLAQSAEELR
                     RAGYPLTKCAIG"
     gene            969..1913
                     /locus_tag="VFW59_04535"
     CDS             969..1913
                     /locus_tag="VFW59_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEX5363512.1"
                     /translation="MSDLLKKIDARTKLAGTNKLEILMFSLGKDMRSGREETFGINVF
                     KVREVMRIPEITRAPEMPSAVEGMVSLRGALVPVIDLAKYIGIEAESKPEIMIVTEYN
                     GHTQGFLVKAVDNILRLDWAAMRVPPAMMVAEMGGLVTAITELKDKRLVMMIDVEKVL
                     SETTHFDSDDLIIKSVRPLGMENRTVFFADDSSVARNQIAKTLDAMQVKHISAINGRQ
                     AWIELSKMADYADATHTPLKDMLQVILTDVEMPEMDGYMLTRKIKADKRFAGIPVLMH
                     SSLSSTSNELMGKAVGVDEYVPKFEPQKLAQALARLLA"
     gene            1924..2871
                     /locus_tag="VFW59_04540"
     CDS             1924..2871
                     /locus_tag="VFW59_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEX5363513.1"
                     /translation="MTYTEDMLNSAEDGLLDHIDARTNLAGTNKMEILLFNLGTDEKF
                     GINVFKVKEVSQAGKITRTPNMPNGVDGIVSLRGHVMPVLNLASFMGIYPEEKHLTMM
                     VAEYNTHILGFLVKEVDRIIRVDWDKVRATEGMLSDKGALITAITELPDGTLVSILDV
                     EQILANAFGEGVVGNVERVESERDLCVFFADDSMVARRKIAEVLDKMGVKHIQANNGR
                     EGWDRLKAMADVAQSTGANLHDQIQVILTDAEMPEMDGYVLTQHIKADRRFDGIPVVM
                     HSSLSSDANRAMGRSVGVDYYVSKFDSLVLSSTLRPLLT"
     gene            2960..3349
                     /gene="cheY"
                     /locus_tag="VFW59_04545"
     CDS             2960..3349
                     /gene="cheY"
                     /locus_tag="VFW59_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEX5363514.1"
                     /translation="MGIEDTKFLVVDDFSTMRRIVRNLLKELGFANVQEAEDGVDALA
                     KLRSDHFDFVVSDWNMPNMTGIELLRTIRADDKLKHLPVLMVTAEAKKENIIEAAQAG
                     ASGYVVKPFTAATLDEKLKKIFQNVQK"
     gene            3358..>3622
                     /locus_tag="VFW59_04550"
     CDS             3358..>3622
                     /locus_tag="VFW59_04550"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEX5363515.1"
                     /translation="MATNTATQDSDDLEALFDSIVAANAEETKVASAPAANAAIADGD
                     PAKVINHIGQMARTLHDTLRELGLNKEIEKAAATIPDARDRLNY"
BASE COUNT          880 a          892 c         1153 g          697 t
ORIGIN      
        1 tatttgcgca ccatggtgga tgagatcgtc acgccggtag cgcatattca ggggcgcatc
       61 agggcgctcg atgagcgggt atgcggggtg gccgacaacg cggtgaacga acaggggcat
      121 atccagacca tcgcttccac gatggaggag ttcagcgaat cggtggccga agtggcgcat
      181 atggcggcgg attcgttgaa cgatgcgaaa tcgatgcaac agaccgtcga ggaaaataat
      241 cgcaatatgg agctcagcat cgaggcgacc agcagggtcg cggatacggt gcaatcatcc
      301 agcagaacga tttccgatct gggcgcgtcc atcgaaaaaa tcggcacgat tgccctcgcg
      361 atcaaggaga tcgccgagca gaccaatttg ctggcgctga atgccgcgat cgaagctgcg
      421 cgcgccgggg agcaggggcg cggcttcgcg gtggtggcgg acgaagtgcg caagctcgcc
      481 gagcgcacgg cggcaagcac caaggatatt gccagcacga tcggcgaaat caatacgatc
      541 tcggatgctg ccgttcatgc gatgaatggc gcggtctccg aagtggaaac cggtatcgcg
      601 ctgatccgca agaacggcga aggcctgaaa gaaatcatgt ccgcgacggt caatgtggcg
      661 aaccgcatcg agcatatcgc gacagcatcg aacgagcagt ctaccgccgg cgagagcgtg
      721 gtgagcagtc tggagcgcat ctccgggctg gtggacagca acacccatgc ggccaaggac
      781 gccaaggcgg aggccgaatc tctggcgcag tccgccgaag agttgaggag ggctggttat
      841 ccgttaacca agtgtgcgat tggataggga acgaacaaca tcggttgtct ggcgctggtt
      901 agcggcgccg caatgtagtc gcgctgacta cctgacgacc ggcagcaaac agcaattagg
      961 agaaacaaat gtctgacctg ttaaaaaaga ttgatgcccg taccaaactg gcgggaacca
     1021 acaagctgga aatcctgatg ttctcgctgg gcaaggatat gcgcagcggg cgcgaggaaa
     1081 ctttcggcat caacgtgttc aaggtgcgcg aggtgatgcg tatccccgag attacccgtg
     1141 cgccggaaat gccgtcggcc gtcgaaggca tggtgagctt gcgcggtgcg ctggttcccg
     1201 tgatcgatct ggccaaatac atcgggatcg aggctgagag caagccggag atcatgatcg
     1261 tcaccgagta caacggccat acgcagggct ttctggtcaa ggcggtggat aacatcctgc
     1321 gcctcgactg ggcggcgatg cgcgtgccac cggcaatgat ggtcgcggaa atgggcggac
     1381 tggttacggc gatcaccgaa ctgaaggaca agcgcctggt gatgatgata gacgtggaaa
     1441 aagtgctgtc ggaaacgacc catttcgata gcgacgacct gattatcaaa agcgtcaggc
     1501 cgctgggcat ggagaacaga accgtgtttt ttgccgacga ctcatcggtg gcgcgcaatc
     1561 agatcgcaaa gactctggat gccatgcagg tgaagcatat ctcggcgatc aatggtcgtc
     1621 aggcctggat cgagctgtcg aaaatggccg attacgccga tgcgacacat acgccgctca
     1681 aggacatgtt gcaggtgatc ctcaccgacg tggagatgcc ggaaatggac ggatatatgc
     1741 tgacgcgcaa gatcaaggcg gacaaacgtt ttgccggcat cccggtgttg atgcactcat
     1801 cgctgagcag cacttccaat gaactgatgg gcaaggctgt cggggtggat gaatatgtgc
     1861 cgaaattcga accgcagaag ctggcacagg ctttagcgcg gttgctggct tgaggagacg
     1921 gaaatgactt atacggaaga catgttgaat agtgccgagg atggtttgct ggatcatatc
     1981 gatgcccgca ccaatctggc cggcaccaac aagatggaga tattgctgtt caatcttggc
     2041 accgacgaaa aattcggcat caatgtgttc aaggtcaagg aggtgagcca ggccgggaag
     2101 attacgcgca cgccgaacat gcctaacggt gtggacggta tcgtcagctt gcgcgggcat
     2161 gtgatgccgg tgttgaacct ggcgagcttc atggggatat atccagaaga aaaacacctg
     2221 accatgatgg tggccgagta caacacgcac atcctcgggt ttttggtgaa agaggttgat
     2281 cgcatcatcc gggttgactg ggacaaggtg cgcgcgaccg aaggcatgct ctccgacaag
     2341 ggcgcgctga ttaccgcgat taccgaattg ccggacggca cgctggtttc gattctggat
     2401 gtcgagcaaa tcctcgccaa cgcgttcggc gaaggcgtgg tcggcaacgt cgagcgcgtc
     2461 gagtccgagc gcgatctgtg cgtgttcttc gcggatgact cgatggtggc gcgccggaaa
     2521 attgccgaag tgctggacaa gatgggcgtc aagcacatcc aggcgaacaa tggccgcgaa
     2581 ggttgggaca ggctcaaagc tatggccgac gtggcgcaaa gcaccggcgc gaatctgcac
     2641 gatcagatcc aggtgatcct gaccgacgcg gaaatgccgg agatggacgg ctatgtgctg
     2701 acccagcaca tcaaggcaga cagacgcttt gacggcatac cggtggtgat gcactcgtcg
     2761 ttgtcatcgg atgcgaatcg cgccatggga cggagcgtcg gtgtcgatta ttacgtgtcg
     2821 aaatttgatt cgctggtgtt gtcgtcaacg ttgcgaccgt tgttgacgta atgcggcagc
     2881 gttattggac atcatgtaga tagtcggctt ttaactgcaa cggacgacag gtagctgacg
     2941 acggatttag gagattgtta tgggaattga agatacaaaa tttttggtgg tggatgattt
     3001 ttcgacgatg cgccgcatcg tgcgaaatct tctcaaggaa ttgggtttcg cgaatgtgca
     3061 ggaggccgaa gacggcgtgg atgcgctggc caagctgcgc tccgatcatt tcgacttcgt
     3121 ggtgtccgac tggaatatgc ccaacatgac gggaatcgag ttgttgcgca ccatccgtgc
     3181 ggacgacaag ctcaaacatt tgccggtatt gatggtgacg gcggaagcta agaaggaaaa
     3241 catcatcgag gcggcgcagg cgggtgcaag cggttatgtg gtgaagccgt ttactgccgc
     3301 tacgctggat gagaagttga agaaaatctt tcaaaacgtg caaaagtgag taatgccatg
     3361 gctaccaata ctgcaactca ggattccgac gacctcgaag cattattcga cagtatcgtc
     3421 gcggcaaacg cggaagaaac caaagtggct tccgcaccag cggcgaacgc cgcaattgcc
     3481 gatggcgatc cggccaaggt tatcaaccat atcggccaga tggcgcgaac tttgcacgat
     3541 acgctgcgcg aactggggct gaacaaggaa atcgaaaaag ccgccgcgac tattccggac
     3601 gcgcgcgacc gtctgaatta tg
//
LOCUS       DASUIV010000120         6994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_586939, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000120 DASUIV010000000
VERSION     DASUIV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586939"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VFW59_04555"
     CDS             complement(<1..1009)
                     /locus_tag="VFW59_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5363516.1"
                     /translation="MTLYAYRAIDNRGKSSKGLQDAANQVDLELRLKHGGLDLIDAKV
                     DNGRMGLARGRIKRPELITFFFNLEQLTRAGVPLLESLADLRDTMSDPRFREIIANLV
                     EAIEGGKKLSQAMTDHPDAFDRTFISLTHAGEESGRLPEVFLHITEALKWQDEMASHT
                     KTILLYPAFVGAIVVAITFFLMIYLVPQLVGFIKSMGQEIPIQTRALLATSAFFVNYW
                     YVLLALPVVLPIAGRLLISSSSEMRYRFDSLKLNLWVIGPILHKIILARFANTFAMMY
                     GSGVNIMECITNSRDVVNNLVVAKSLDDVMLEIESGKNLTQSFQSTGMFPPLVIRMLK
                     VG"
     gene            complement(1011..2717)
                     /locus_tag="VFW59_04560"
     CDS             complement(1011..2717)
                     /locus_tag="VFW59_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5363517.1"
                     /translation="MQNQDNQLQQPLGRILADKGVISDDQLRIALQEQNKTHQPLGRL
                     LVRLGFLSEATIRDVLSENLGQESVDLSTIIVDPAALSIIPKDVARRYQLLPLSVDKK
                     ERVLTLAVADPDNIIALDQVRALLKDEYRLITQLASESDILRAIDQYYGFELSIDGIL
                     HEIEPGEMEYQTQQGVNEFSQPVVRLIDALLTEAVQRGVSDIHFEPESSFLRIRYRVD
                     GVLRQVRSLHKSFWPAMVVRLKVMSNMNIAETRAPQDGRISLRLSGRQIDFRVASHPT
                     THGENIVLRILDRQKGIVQLNRIGLDDETLNTLKMIIAKPEGIVLVTGPTGSGKTTTL
                     YSVLNHINTESVNIMTLEDPVEYPIAMIRQTSINESAKLDFANGIRSMMRQDPDIILV
                     GEIRDHPTAEMAFRAAMTGHQVYSTLHTNSAIGAIPRLLDIGILPDIMAGNIIGIVAQ
                     RLVRVLCKECKYPYQPDAQERKLLGIPAHQDITLYRAAGCDACHHQGYSGRMAIMELL
                     KMDHDLDDLVSRRASTRQIRETALEKGFRPLAMDGIRRILQGVTSLAEVSRLIDLTDR
                     AN"
     gene            complement(2722..3780)
                     /locus_tag="VFW59_04565"
     CDS             complement(2722..3780)
                     /locus_tag="VFW59_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5363518.1"
                     /translation="MSLLLDARKKSLRAQHAQAAERASDAIEAAAPTARDAGRNLFAA
                     KTASAGFALPSRGLMIALAATILLLAAGLGYLWRLDSQSGDLVPLHPMPPHIALSSPA
                     LTGSSSTPANRSEAALPGGDMPQADQPAAVAGAMERQAPQRDEQNPPHAVNPVRVEHQ
                     RTETIDPLLERAYLTYRVGNLEEAQQLYQTMLAKDTRNADAQLGLAAIAQQRGENMLA
                     AHYYASVLTLDPRNAVANAGMSALGADNEGDENRLRALLREQNNSAALHFALGNLLIA
                     QSRWGEAQQAYFNAYTLEPDNAEIAYNLAVSLDHLGQSKLAAQYYQRALQLDTADNSS
                     FDHALVARRIERLGQPGK"
     gene            complement(3782..5545)
                     /gene="mshL"
                     /locus_tag="VFW59_04570"
     CDS             complement(3782..5545)
                     /gene="mshL"
                     /locus_tag="VFW59_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HEX5363519.1"
                     /translation="MPPRNIFRLCTLLCAAGAIVVGCGTRPIQPSDKHIQQTPDQSVA
                     ADIPAPLTRNIALPAPKASPRTETYSVVVTGVPAQEILFALARDAKINLDIHQGIQGT
                     VTLNALNQTLPQILTRIAKQVEMRYELDNGNLIVMPDSPYLHNYKIDYVNMTRDAEST
                     INNSMQVSNTGGVAIATGPGANTSSISIKNISKNRFWDTLVQNIKDILRETDKILPDG
                     SSETVTEQSGSTDHIVTDKKNTIRKNGNETTPTQTVIQEGDSKLITRRSTFREAASVI
                     ANPENGIITVRATGRQHEKIQEFVDQVMNNARRQVMIEATIVEVRLNDQYKQGINWSA
                     IIKANGKEFKLLQSSAGLPTAVGNGVFGIITNPTATTVTQQVSLLESFGNVKVLSSPK
                     LSVLNNQTAMMRVVDNLVYFTVKADTITNQNTAAVTTYTTTPNTIPVGFTMSITPQIS
                     DSDVVLLNLKPSISRVIRYVPDPNPNLSAANNQGIPETQTREMESVMRMENNQIAVMG
                     GLMQDSINNLTDAVPGLSRLPLAGEFFKQRNDTTTKTELVIFLRPIVVKDASITGDFN
                     AYRNLLPDQNFFKEPVKNGKP"
     gene            complement(5629..>6994)
                     /locus_tag="VFW59_04575"
     CDS             complement(5629..>6994)
                     /locus_tag="VFW59_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5363520.1"
                     /translation="NHFGLNEAPFRITPATTFFFSGANRGEILDALIYSLSEVEGIIK
                     VSGEVGSGKTMLCRMLLERLPEHIETIYLANPSLSREEMLYAIADGLGLNVNGERVGV
                     IMHGIQDKLEEKAREGRRVVVLVDEAHAMPPETLEELRLLYNLQVGDFKLLQIILFGQ
                     PELNTKLEQPNMRQLKDRIVHHFNMQPISRNTLESYLMFRMRAAGFHGPNIFSPEALK
                     LIARASDGLMRRVNILADKSLLSAFVEDTHGIEARHVQAAMRDSELQTSRKLADKKLL
                     AGGAVTAMLLTGLAVWQFLGGSGTSSEKPLQPPSAEPMIAASADNTRTEKTAATAQPI
                     AASPALRAPQAELPHTTARGSLFDQRLAAGKQLTEQKNIVASIQLFYNEGVQPSRLEG
                     FLKRADSLGRLPSIYLLPARTGQKDGIRVLYGAYPSVEAARSAIKDLPARYQESFATS
                     IYLF"
BASE COUNT         1206 a         1882 c         2272 g         1634 t
ORIGIN      
        1 cacccacctt gagcatgcgt atcaccaggg gggggaacat gccggtgctc tggaagcttt
       61 gcgtgagatt cttgccggat tcgatttcca gcatgacatc gtccaggctc ttggctacca
      121 ccaggttgtt gaccacatcg cgggaattgg tgatgcactc catgatgttc acgcccgatc
      181 cgtacatcat cgcgaaggtg ttggcgaagc gcgccagaat gatcttgtgc agaatggggc
      241 cgattaccca gagattgagt ttcaggctgt cgaagcggta gcgcatctcc gaactggagg
      301 agatcagcaa ccttcctgcg atgggcaaaa cgacaggcag ggcgagcagc acataccagt
      361 agtttacgaa aaaggccgaa gtggcgagca gggcgcgggt ctggatcgga atttcctgtc
      421 ccatgctttt gatgaagccg accagttgcg gcaccagata gatcatcagg aaaaaagtga
      481 tggccaccac gatggcgccg acaaaggccg ggtagagcaa gatggtcttg gtgtgcgaag
      541 ccatctcgtc ctgccattta agtgcctcgg tgatatgcag aaaaacttca ggcaagcgcc
      601 cgctctcttc gcccgcatgg gtgaggctga tgaaggttcg gtcgaatgca tccgggtgat
      661 ccgtcatggc ttgtgaaagt ttcttgcctc cctcgatggc ttcgaccaga ttggcgatga
      721 tctcccgaaa gcgcggatcg ctcatggtgt cgcgcaggtc ggccagactt tccagcaggg
      781 gaaccccggc gcgagtgagc tgctcgaggt tgaagaagaa ggtgatcagt tccgggcgtt
      841 tgattctgcc gcgcgccaaa cccatacggc cgttgtccac tttggcgtcg atcaggtcaa
      901 ggccgccgtg cttcaggcgc aattccagat ccacctgatt ggccgcatcc tgcaatccct
      961 tgctggattt gccccggttg tcgatggcgc ggtaggcgta aagcgtcatt tcaattcgcc
     1021 cggtcggtca gatcgatcag gcggctgacc tctgcaagcg aggtgacgcc ttgcaggatg
     1081 cggcggatac cgtccatcgc cagcgggcga aatcctttct ccagcgcggt ctcgcgtatc
     1141 tgtctggtgc tggcgcggcg cgataccaga tcgtccagat cgtgatccat cttgagcagt
     1201 tccatgatgg ccatccgtcc gctgtagccc tggtgatggc aggcatcgca gcctgcggcg
     1261 cgatacaggg tgatgtcctg atgggcggga atgcccagca gtttgcgttc ctgagcatcc
     1321 ggttgatagg gatatttgca ttccttgcac agcacgcgaa ccagacgctg cgccacgatg
     1381 ccgatgatgt tgccagccat gatgtccggc aggatgccga tgtcgagcag gcgcggtatc
     1441 gcgccgatgg ccgagttggt gtgcagcgtg gaatacacct gatgtcccgt catggcggcg
     1501 cggaatgcca tctcggcggt cgggtggtcg cggatctcac ccaccaggat gatgtccgga
     1561 tcctgccgca tcatcgaacg tatgccgttg gcaaagtcca gtttggcgga ttcgttgatc
     1621 gaggtctggc ggatcatcgc gatcggatat tccaccgggt cttccagcgt catgatgttg
     1681 actgactcgg tgttgatgtg attgagcacc gagtacagcg tggtggtctt gccgctgccg
     1741 gtagggccgg tgaccagcac gatgccttcg ggtttggcga tgatcatctt gagcgtattg
     1801 agcgtctcgt catccaggcc gatacggttg agctgcacga tgcccttctg cctgtccaga
     1861 atgcgcagca cgatgttctc gccgtgcgtg gtgggatgcg aggccacccg gaaatcgatc
     1921 tggcggccgg acaggcgcag cgagatgcgg ccatcctgcg gtgcgcgtgt ttcggcgatg
     1981 ttcatgttgc tcatcacctt caggcgtacc accatcgccg gccagaagct cttgtgcagg
     2041 ctgcgcacct ggcgcaacac cccgtcgacg cggtagcgga tgcgcaggaa actgctttcc
     2101 ggttcgaagt gaatgtcgga gaccccgcgc tgtaccgctt cggtcagcag cgcatcgatc
     2161 aggcgtacca cgggctggct gaattcgttg acgccttgct gcgtctggta ctccatctcg
     2221 ccgggttcga tctcgtgcag gatgccgtcg atcgagagtt cgaagccgta gtactggtcg
     2281 atcgcgcgca ggatgtcgga ttcgctcgcc agctgggtga tcaggcggta ctcgtccttg
     2341 agcagagcgc gcacctggtc gagcgcgatg atgttgtccg ggtcggcgac cgccagagtc
     2401 agcacacgct ctttcttgtc taccgaaagg ggcagcagtt ggtagcgccg ggcgacatcc
     2461 ttgggaatga tggacagcgc ggcgggatcg acgatgatgg tggagaggtc gacactctcc
     2521 tggcccagat tctccgatag cacgtcgcgg atggtcgctt cggagaggaa gcccaggcgc
     2581 accagcaagc ggcccaaggg ctggtgcgtt ttgttttgtt cctgcagcgc gatgcgcaac
     2641 tgatcgtcgc tgatcacccc tttgtcggcg agtatgcgcc caagaggttg ctgcagctgg
     2701 ttgtcctggt tttgcatgtg attacttgcc tggttgaccc agcctctcga tacgtcgcgc
     2761 gaccagtgca tggtcgaaac tcgagttatc tgccgtatcg agttgcaatg cgcgttggta
     2821 gtattgagca gccagtttgc tctggccgag gtgatcgagg ctgacggcga gattgtaggc
     2881 gatttcggcg ttatccggct ccagcgtata ggcattgaag taggcctgtt gcgcttcacc
     2941 ccagcgcgac tgagcgatga gcagattccc cagcgcgaag tgcagtgcgg ccgagttgtt
     3001 ctgctcgcgc aacagggctc tcaggcgatt ctcgtcgccc tcgttgtctg cgcctagcgc
     3061 cgacatgccc gcgttcgcca cggcattgcg cggatccagt gtcagtacgc ttgcataata
     3121 atgtgctgcc agcatgttct cgccgcgttg ctgggcaatg gcagccaggc cgagctgcgc
     3181 gtcggcgttg cgtgtgtcct tggcaagcat ggtctgatat agctgctgtg cctcttccag
     3241 attgccgaca cggtaggtga gataggctct ttcgagcaac ggatcgatgg tttcggttcg
     3301 ctggtgttcg actcgcaccg gattgacggc atgtggcgga ttctgttcat cccgttgggg
     3361 ggcttgccgc tccatggcgc ccgctacggc agcaggttga tccgcctgtg gcatgtcgcc
     3421 gccggggagg gcggcttcgc tccggttggc cggcgtgctt gaacttcccg tgagcgcggg
     3481 cgatgacagt gcgatgtggg ggggcatcgg gtgcaggggg acgaggtcgc cgctctggct
     3541 gtcgaggcgc catagatagc ccaagcctgc ggcgagcagc agtatcgtgg cggccagcgc
     3601 gatcatcagg ccgcgagacg gcagggcgaa tccggcagag gcggttttgg ccgcgaacag
     3661 attgcggccc gcatcccttg ctgttggcgc ggctgcttcg atggcatcag atgcgcgttc
     3721 cgccgcctgc gcgtgttgcg cccgcagaga tttttttcgt gcatccagaa gcaggctcat
     3781 gtcagggttt gccgtttttt accggctcct taaagaagtt ttgatcgggc agcaggttgc
     3841 gataggcgtt gaagtcgccg gtgatgctgg catccttgac gacgatcggg cgcaggaaaa
     3901 tcaccagttc ggttttggtg gtcgtgtcat tgcgctgctt gaagaattca ccggcgagcg
     3961 gcaggcggga gagcccgggc acggcatcgg tcaggttatt gatcgagtcc tgcatcagtc
     4021 cgcccatgac ggcgatctgg ttgttctcca tgcgcatcac cgactccatc tcacgcgtct
     4081 gggtctccgg aatgccctga ttgttggctg cgctcagatt ggggttgggg tcggggacat
     4141 agcggatgac gcgcgagatc gatggcttga ggttgagcag taccacatcg ctgtcgctga
     4201 tctgcggcgt gatgctcatg gtgaaaccga ccgggatcgt gttcggggtg gttgtgtaag
     4261 tggtgaccgc tgcggtgttc tggttggtga tcgtgtccgc tttaaccgtg aagtagacca
     4321 gattgtccac tactcgcatc atcgcggtct ggttgttgag cacgctcagt ttcgggctgg
     4381 acagcacctt gacgttgccg aaggattcca gcaacgagac ctgttgggta acggtggtgg
     4441 cggtcgggtt ggtgatgatg ccgaatacgc cgttgccgac cgcagtgggc agaccggcgc
     4501 tgctttgcag cagtttgaat tccttgccat tggccttgat gatggccgac cagttgatgc
     4561 cctgtttgta ttggtcgtta aggcgaacct cgacgatggt cgcctctatc atcacctggc
     4621 ggcgcgcatt gttcatcacc tgatcgacga actcctgaat cttttcgtgc tgcctgcctg
     4681 tggcgcgtac cgtgatgatg ccgttctccg gattggcgat gaccgaggct gcttcacgga
     4741 aggtgctgcg acgggtgatg agtttgctgt cgccttcctg aatgacggtt tgcgtcggag
     4801 tcgtctcgtt gccgttcttc ctgatcgtat tcttcttgtc ggtcacgatg tggtcggtgc
     4861 tgccgctttg ttcggtcacg gtctcgctcg atccgtcggg cagtatcttg tcggtctcgc
     4921 gcaggatgtc cttgatgttc tgcacgagcg tatcccagaa gcggttcttc gagatgttct
     4981 tgatcgagat cgacgaggtg ttcgcgccgg ggccggttgc gatggcgacc ccgccggtgt
     5041 tgctgacctg catggaattg ttgatcgtgc tctccgcatc gcgggtcatg ttcacgtagt
     5101 cgatcttgta gttgtgcaga tagggagagt ccggcatcac gatcagattg ccattgtcca
     5161 gttcatagcg catctccacc tgtttggcga tgcgggtgag gatctgcggc agcgtctggt
     5221 tgagtgcatt cagggtgacc gtcccctgta tcccctgatg gatatccagg ttgatttttg
     5281 catcgcgcgc caaggcgaac agaatctcct gggcgggaac gcccgtgacg acgacgctat
     5341 aagtttcggt tctggggctg gctttggggg cgggaagtgc gatgttgcgc gtgagtggtg
     5401 cggggatgtc tgctgcaacg ctctgatccg gagtttgctg gatatgtttg tccgatggct
     5461 ggatgggcct ggtgccgcag ccgacaacga ttgcgcctgc ggcgcagagg agggtacaga
     5521 ggcggaatat gttgcggggg ggcatcactt ttctccatta cagccgttcg aatgcagcct
     5581 aacagttttg ccttgtccgc tcaacagatt gttcccttca cgtacctctc agaacagata
     5641 gatggaggtg gcgaaggatt cttgatagcg cgccggaaga tccttgatcg cgctgcgtgc
     5701 cgcttcgacc gaggggtagg ctccgtatag cacacggatg ccgtccttct ggccggttct
     5761 ggcaggtagc agatagatgc tcgggagtct gcccaggctg tctgcgcgtt tgaggaagcc
     5821 ttccaggcgt gagggttgga ccccttcgtt gtaaaagagc tggatgctgg cgacgatgtt
     5881 tttttgttcg gtcagttgtt ttccggccgc taaccgttga tcgaaaagcg agcccctggc
     5941 tgttgtatgg ggcaactcgg cttgcggcgc ccgcagtgcc ggactggcag cgatgggttg
     6001 cgctgtagcc gccgttttct ccgtccgggt gttatccgcc gatgccgcga tcatcggttc
     6061 ggcggagggg ggctgcaggg gcttttcgga agaagtgccg gaaccgccca gaaactgcca
     6121 gacggcgagg ccggttaaca gcatggcagt gaccgctccg cctgccagca gttttttgtc
     6181 ggctaacttg cgggatgtct ggagttcgct gtcgcgcatc gcggcctgaa catggcgcgc
     6241 ttcgatgccg tgagtatctt caacgaaggc agacagcagt gatttgtccg ccaggatgtt
     6301 gacgcgacgc atcaagccgt cggaggcgcg tgcgataagt ttcagtgcct cgggcgagaa
     6361 gatgttgggg ccatggaagc cggcggcccg catgcgaaac atcaaatagc tttccagcgt
     6421 gttgcgcgat atcggctgca tgttgaagtg gtgcacgatg cgatccttga gctggcgcat
     6481 gttgggctgc tccagcttgg tgttcagttc cggctgcccg aacaggatga tctgcagcag
     6541 cttgaaatca ccgacttgca gattgtagag caggcgcaat tcttccagcg tttccggtgg
     6601 catggcatga gcctcgtcga ccagaacgac cacgcgtctg ccttcgcggg ctttttcttc
     6661 cagcttgtcc tggatgccgt gcatgatgac gccgacgcgc tcgccgttaa cgttcagtcc
     6721 caggccgtct gcgattgcgt acagcatctc ttcgcgtgac aggctgggat tggcgagata
     6781 aatagtttcg atatgttcgg gcaggcgttc cagcaacatg cggcacagca tggtcttgcc
     6841 gctgccgacc tcgccgctga ccttgatgat tccttcgacc tcgctcagcg agtagatcag
     6901 cgcatcgaga atctcgccac ggttggcgcc ggaaaagaag aaggtcgtgg cgggcgtgat
     6961 cctgaatggc gcttcattga gtccgaaatg attg
//
LOCUS       DASUIV010000121         7822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_958724, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000121 DASUIV010000000
VERSION     DASUIV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958724"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFW59_04580"
     CDS             <1..686
                     /locus_tag="VFW59_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5363521.1"
                     /translation="THLELNRLRNHLEFLVEERTAELRKSEKTLRASLLDSIAALAAT
                     VEIRDPYTAGHQNRVAQLAVAIARELQLPEEQVEGIHLASIVHDVGKIQVPGELLSKP
                     GKLSALEFDLIKEHAKAGYEILKPIAFPWPIAQIVLQHHERLDGSGYPQGLRRGQILP
                     EACILAVADVVESMMSHRPYRPGLGLDAALGQVAVNKGTLFDPVIVDACLKLFQERGF
                     HFSNGILPR"
     gene            799..2058
                     /locus_tag="VFW59_04585"
     CDS             799..2058
                     /locus_tag="VFW59_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX5363522.1"
                     /translation="MRYHRGITVVIAGTGINLALGILYSWSVFKESIKSAIAAGGEGA
                     FTWNPASINDPYALACLVFAFTMIPAGRAQDRFGPRVAAFIGGVLVSMGFLLISQSVN
                     YWVWVIGFGGMVGAGLAFGYSAATPAALKWFPPGKSGMVAGIVVSGFGLAPAYIAPLS
                     TYLVSTHGLLYTMLFFGIAFLAVVSILSRFLILPPADYKPAAPPSRRSDEQLRASFPQ
                     SDETPQSLLKKRQFWALWLLYFIGAGAGLMVIGSLSGLAKSSMGDQAYLAVAILAIGN
                     ASGRVLAGMLSDRFGRKKVLAGIFLFQALLMFGAAYASQTSGAVLVVLVATLIGFNYG
                     ANLAIFPSYAKDLWGMPHFGINYGMLFTAWGVGGFVMVKLSEILKAQSGSFTLSFLAA
                     GGLLLTGLVLTLRLDDQKEQARVALRS"
     gene            complement(2134..4704)
                     /gene="cphA"
                     /locus_tag="VFW59_04590"
     CDS             complement(2134..4704)
                     /gene="cphA"
                     /locus_tag="VFW59_04590"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5363523.1"
                     /translation="MEISRIRALRGPNLWSRHTAIEAIVSCVDSDAISVLHGFEVRLR
                     ERFPGIGQLHPAGRDEPITLAHALGLAALGLQAHAGCPVSFSRTAQTVEAGIFQVIVE
                     YTEEAVGRLALDLAQALLRAAVDDTPFDLADALHQLRELDEDVRLGPSTGAIVQAAIT
                     RGIPCRRLTEGSLVQFGWGSRQRRIQAAETDRTSAIAEAIAQDKNLTKELLDAAGVPV
                     PVGRPVSDEEDAWAAACEIGLPVVVKPVDGNQGKGVTVNVASREHLHIAYTAAAEYGS
                     EVMIERYLPGYDFRLLVVGDQVVAAARRDPPHVIGDGEHTIHELVEMVNSDPLRGNGH
                     ATSLTKIRFDEIAHARLALQDFDENSIPSKGTRVVLRNNANLSTGGTATDVTDDVHPE
                     LAARAVAAARMVGLDICGVDIVCDSVVQPLEAQNGGVVEVNAAPGLRMHLQPSFGKGR
                     AVGEAIVASMFAPGEDGRIPVIAVAGTNGKTTTVRLIAHMLTLNGKNVGMTNSDGVYI
                     GKRRIDSGDCSGPKSARNVLMHPDVDAAVLETARGGVLREGLAFDRCDVAVVTNIGMG
                     DHLGLSYISTVEDLAVVKRVIVQNVSPSGMAVLNALDPMAAAMADACPAPVTFFAADA
                     HHPIAATQRAQGRRLVCLDGDSILAVEGDMEQRVRLADIPITRNGTIGFQIENAMAAT
                     AAGWALGLSWDSIRSALSSFVNDAQTAPGRFNLFNYRGATVIADYGHNPDAILALVAA
                     IDNMPAQRRVVVISGAGDRRDVDIRRQTEILGDAFDEVILYQDQCQRGRADGEVLALL
                     RQGLEHAQRTTKVSEIHGEFLATDSALAQLKSGDLCLILIDQVDEALAHIARRVAEAS
                     "
     gene            complement(4762..6894)
                     /gene="cphA"
                     /locus_tag="VFW59_04595"
     CDS             complement(4762..6894)
                     /gene="cphA"
                     /locus_tag="VFW59_04595"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5363524.1"
                     /translation="MKILRITHLRGPNIWTYRPVLEAWVDIGTLEDAPSNTIPGFYER
                     LSSWLPTLIEHRCGIGERGGFLQRLREGTWAAHVLEHVTLELQNLAGMQSGFGKARET
                     SARGIYKVAIRSRHEQVSHTAFLAARELVLAAMEDRPYDVSATVANLRDMVDSLCLGP
                     STACIVDAASARRIPSNRLTEGNLVQLGQGVRSRRIWTAETDLTSAIGETISRDKDLT
                     KNLLQSCGVPVPEGHIVASPEDAWESALDIGLPVVIKPVDGNHGRGVSTDLKTRAEIE
                     AAYHLAIAEGSEVIVERFIRGNEHRLLVVGRQVVAAARGEIATVTGDGKLTIVELIDQ
                     QINSDPRRATTEDYPLNLVLIENDAAVRLELERQGYRENSVPASGIEVLIQRNGNVAF
                     DVTDQLHPEVAEMAALAARIVGLDIAGVDLVAEDISRPLAEQGGAIVEVNAGPGLLMH
                     LKPSNGGPRPVGSAIVEHLFPNHETGRIPVVGITGTSGTTSVARLVAHLLQLSRLRTG
                     LACDDGLFFDQRRIEAGERGNWASGHKLLLNRAAEAAVFENGARALLSEGLAYDRCHV
                     GVVTDLLHAEGLAEFYIHDTEQLYNVLRTQIDVVLPDGAAVLNADDPLVARMAELCDG
                     EVIFFGKDGSPVIAAHRATGGRAVSMRGKQIAIAKGAEEIGVIDLARPMLSEQRSEDI
                     AAAVGAAWALKIPIDVIRAGVETLTTVL"
     gene            7161..>7822
                     /locus_tag="VFW59_04600"
     CDS             7161..>7822
                     /locus_tag="VFW59_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX5363525.1"
                     /translation="MLRATPSLQDGEHALAWLDTDLDAALRFAPGIVLVTERRILALP
                     AGAVDWQDWSYRAGLVLQHHDHAGVGTLELLDGQGCLARWRYTLGENLAALRLIAQFE
                     RRLSSHLSGQPVAEETQAVCPKCKTALPSGEDECPICARTIDAPPSTWTLLRLWRFAK
                     PYRNQLLAGFLLTLAATAATLVPPYLVMPLMDKVLIPYQNGKPIDTGLVTLLLAGLFG
                     SAL"
BASE COUNT         1348 a         2395 c         2465 g         1614 t
ORIGIN      
        1 atacccatct ggagttgaat cggctgcgca atcacctgga gtttctggtc gaagagcgta
       61 ccgccgagct gaggaaaagc gagaagacgt tgagagccag tttgcttgac tccatcgcgg
      121 cccttgctgc caccgtcgaa attcgcgatc cctataccgc tggacatcag aaccgtgtcg
      181 cacaactcgc cgtcgcaatc gccagagagc tgcaactccc ggaggagcaa gtcgagggga
      241 tacacttggc cagcatcgtg cacgatgtcg gcaagattca ggttccgggt gaactgctga
      301 gcaagccggg gaaactcagt gcattggaat tcgatctcat caaggaacac gccaaggccg
      361 gctacgagat actcaaaccg atcgcctttc catggccgat cgcgcaaatc gtattgcagc
      421 accacgaacg gcttgatgga tcgggctacc cgcaggggct aaggcgcggg cagatcctgc
      481 cggaggcctg tattcttgcg gtagccgatg tggtggagtc gatgatgtcg caccgtcctt
      541 atcggcccgg gctggggctc gatgccgcat tggggcaggt tgccgttaac aagggcacgc
      601 tattcgaccc cgtcatcgtg gatgcctgtt tgaagctttt tcaggaacgg ggctttcact
      661 tttccaacgg aatacttcct cgttaggagc tcgctatgga cggcgtgtca gcaggaacag
      721 gcgcgatcga ggaaacattg ccgcgtaact ctggcgtctg atatcgccga tttcatgccg
      781 taacttagga gggatgcaat gagatatcac cgaggcatta ccgtcgtcat agcgggcaca
      841 ggtatcaatc tggcgctggg catactctac agctggagtg tcttcaagga gtccatcaag
      901 agtgccattg ccgcaggtgg cgagggcgcg ttcacctgga acccggcctc gatcaatgac
      961 ccctatgccc tggcctgtct ggtgttcgca ttcaccatga ttcctgcggg gcgggctcag
     1021 gatagattcg gcccgcgtgt tgcggccttc atcggtggcg tgctggtcag tatgggtttt
     1081 ttgctgatct cccagtcggt gaattactgg gtatgggtaa tcggtttcgg tggcatggtc
     1141 ggtgcggggc tggcctttgg gtattctgcc gcaacacctg ccgcgctcaa atggtttcct
     1201 cctggcaaga gtggcatggt cgcaggtatc gtggtgtcgg gattcggtct cgctccggct
     1261 tacatcgcac cgttgtccac ctatctggtc agcacgcatg gattgctcta taccatgctg
     1321 ttcttcggta tcgccttcct cgcggtggtg tcgattctgt cgcggttcct gatcttgccc
     1381 cccgccgact acaagcccgc agccccacct agtcgacgca gtgacgagca gctgcgggca
     1441 agttttcccc agagcgacga gacgccgcaa agcctgttga aaaaacgcca gttctgggcg
     1501 ttgtggttgc tgtacttcat cggtgcaggt gccgggttga tggtcatcgg cagcctatcg
     1561 gggctggcga agtcgagcat gggcgatcaa gcctatctgg cagttgcgat cctcgccatt
     1621 ggcaatgcct ccggacgcgt gctggcgggc atgctgtcgg atagattcgg acgcaaaaag
     1681 gtgctggctg gcatcttcct gtttcaggcg ctattgatgt tcggtgcagc ctacgccagc
     1741 cagaccagcg gtgccgtgct ggtggtgctg gtcgccacgc tgatcggttt taactacggc
     1801 gccaatctgg ccatctttcc cagctacgcc aaagacctgt ggggcatgcc gcactttggc
     1861 atcaattacg gcatgttgtt caccgcctgg ggcgtgggtg ggtttgtcat ggtcaagctc
     1921 tcggaaatac tcaaggcgca gtcgggttcg ttcaccttgt cttttctggc ggcgggcggt
     1981 ttgctgctaa ccggcctcgt gttgacgctc aggctggacg atcagaaaga gcaggcacgg
     2041 gtcgccttgc gtagttgatc tcttgcacac tccagcatga aaaaacgccc cagagggcgt
     2101 tttttcttta caggtgttac ggtttaatac ggatcaggac gcttcggcca ccctgcgtgc
     2161 gatatgcgcc aaggcttcgt cgacctgatc gatcaggatc aggcacaggt cgccggattt
     2221 gagctgtgcc agcgcgctat cggtggcgag gaattcaccg tgtatctcgc tgactttcgt
     2281 ggtgcgttgc gcatgctcca ggccctgccg cagcaatgcc agcacctcgc catcggcgcg
     2341 gccccgctgg cactggtcct gatacaggat cacttcatcg aaggcatctc ccagtatctc
     2401 ggtctggcgg cggatgtcga catcgcgtcg gtcacccgcg ccgctgatca ccaccacgcg
     2461 gcgttgtgcc ggcatgttgt cgatggcggc gaccagagcc aggatcgcat cggggttgtg
     2521 accataatcg gcgatcacgg tcgcgccacg gtaattgaac aggttgaacc ggccgggggc
     2581 ggtctgcgcg tcattgacga aactggataa cgcgctacgg atggagtccc agctcagtcc
     2641 cagtgcccag cccgctgcgg ttgcggccat cgcattctca atctgaaaac cgatggtgcc
     2701 gttacgggtg atcgggatgt ccgccagccg gacgcgctgt tccatgtcgc cttcgacggc
     2761 caggatgcta tccccgtcca gacaaaccag acgccggcct tgcgcacgct gagtggcggc
     2821 gatcggatgg tgtgcgtcgg cggcaaagaa ggtgaccggg gcggggcagg catcggccat
     2881 cgctgcggcc atcggatcca gtgcattgag caccgccatg ccgctgggag aaacgttttg
     2941 cacgatgacg cgtttgacca cggccagatc ttccaccgtg ctgatgtagc tcagtcccag
     3001 atgatcgccc atgccgatgt tggtgaccac ggccacgtcg cagcgatcga acgccaggcc
     3061 ttcgcgcaaa acgccgccgc gcgcggtctc cagcacggcg gcatcgacat cggggtgcat
     3121 cagcacattg cgcgcgctct tcgggccgct gcagtcaccg ctgtcgatgc gccgcttgcc
     3181 gatatagacc ccatcggaat tggtcatgcc gacatttttt ccgttcaggg tcagcatgtg
     3241 cgcgatcagg cgcaccgtgg tggtcttgcc gttggtgccc gctaccgcga tcaccggaat
     3301 gcgcccgtcc tcgccgggcg cgaacatact ggcgacgatg gcctcgccga ccgcgcgccc
     3361 cttgccgaat gagggttgca aatgcatgcg cagacccggc gcggcattga cttcgaccac
     3421 gccgccgttc tgcgcttcca gcggttgcac cacgctgtcg cacacgatgt cgacgccgca
     3481 gatatccagc cctaccatcc ttgcggcagc gactgcgcgg gctgccagtt cgggatggac
     3541 gtcgtcggtc acatcggtgg cggtgccgcc tgtggacagg ttggcgttat tgcgcagcac
     3601 gacgcgtgtg cccttggacg gtatcgaatt ttcgtcaaag tcttgcagcg ccagtcgcgc
     3661 atgggcgatc tcgtcgaagc ggatcttggt gagcgaggtc gcatgtccat tgccgcgaag
     3721 cggatcgctg ttgaccatct ccaccagttc atggatggtg tgttcgccat cgccgatgac
     3781 atgcggcgga tcgcggcgtg cggcggcaac gacctgatcg cccaccacca gcagacgaaa
     3841 atcgtaaccg ggcaggtaac gctcgatcat gacctcggag ccgtattcgg ccgccgcagt
     3901 gtaggcgatg tgcaaatgtt cgcggctggc gacattgacg gtgacgccct tgccctggtt
     3961 gccgtcgacg ggtttcacca cgaccggcaa accgatctcg caggctgccg cccaggcatc
     4021 ctcttcatcc gataccggcc tgcctaccgg tacggggaca ccggctgcat cgagcagttc
     4081 cttggtcaga ttcttgtcct gcgcgatggc ttcggcgata gcgctggtgc gatcggtctc
     4141 cgccgcctga atgcgacgct ggcggctgcc ccagccgaac tgcaccaggc tgccttcggt
     4201 caggcggcga caggggatgc cgcgcgtgat ggcggcttgc acgatggcac ccgtgcttgg
     4261 gcccagacgc acatcctcgt ccagttcgcg caattggtgc agtgcatcgg cgagatcgaa
     4321 cggcgtgtcg tcgacggcgg cgcgcaacag ggcctgcgcc aaatcgagcg ccaggcggcc
     4381 aacggcttct tcggtatatt cgacgatgac ctgaaagatt ccggcctcga ccgtttgtgc
     4441 ggtgcggctg aaactgaccg ggcagcctgc atgcgcctgc aatcccagcg ccgcgagtcc
     4501 caatgcgtga gccagcgtga tcggttcgtc gcgccccgca ggatggagct ggcctatgcc
     4561 ggggaagcgt tcgcgcaggc gaacttcgaa accgtgaagg acgctgattg cgtcgctgtc
     4621 gacgcaggac acgatggcct cgattgccgt gtggcggctc cacaggttgg ggccgcgcag
     4681 cgcccggata cgcgatattt ccataatttt ccttaacact gccctgagcg catgacggct
     4741 caatttcaag attttgagcc gctacaaaac tgtggttaac gtttcaacgc ccgcgcggat
     4801 gacatcgatc ggaattttaa gcgcccaggc cgcgccgact gcggcggcga tatcttcgct
     4861 gcgctgctcc gacagcatgg gtctggcgag atcgatcacg ccgatctctt cggcaccctt
     4921 ggcgattgcg atctgcttgc cgcgcatgga cacggcccgt cctcccgtgg ctcggtgcgc
     4981 ggcgataacg ggggaaccgt ctttgccgaa gaagatcact tcgccgtcgc agagttccgc
     5041 catgcgtgcc accagcgggt cgtccgcgtt gagcaccgcc gcgccgtccg gcagcaccac
     5101 atcgatctgg gtgcgcagca cgttatagag ctgctcggta tcgtggatgt aaaactcggc
     5161 cagcccctcg gcatggagca ggtcggtgac cacgccgacg tggcagcgat cgtaggccag
     5221 cccttcgctc agcagggcgc gagcgccgtt ttcgaatacg gcggcttcgg ctgcgcggtt
     5281 gagcagcagc ttgtggccgg aggcccagtt gccgcgctcg cccgcttcga tccggcgctg
     5341 gtcgaagaac agtccgtcgt cgcaggccag tccagtgcgc aagcgggata gctgcaacag
     5401 atgggcaacc agtcgtgcaa ccgaagtcgt gcccgatgtg ccggtgatgc cgacgacggg
     5461 gatgcgcccg gtttcgtggt tcgggaacag atgttcgacg atggcgctgc ccaccgggcg
     5521 cggcccgccg ttcgacggct tcaggtgcat cagcagaccc ggcccggcat tgacttcgac
     5581 aatggccccg ccttgttcgg ccagcgggcg cgaaatatct tccgccacca gatcgacccc
     5641 ggcgatgtcc agtcccacga tgcgcgctgc cagcgccgcc atctcggcga cctcggggtg
     5701 cagttgatcg gtcacatcga aagcgacgtt gccgttgcgc tggatcagta cctctatacc
     5761 cgaggcgggc accgaattct cccgatagcc ctggcgttcc agttccagcc tgaccgccgc
     5821 atcattctcg atcagcacga gattgagcgg atagtcctcg gtcgtggcgc gtcgcggatc
     5881 gctattgatc tgctggtcga tgagttcgac gatagtcaat ttgccgtcgc ccgttaccgt
     5941 cgcgatctcg ccgcgcgcgg cggccaccac ctgtcttccg accaccagca ggcggtgttc
     6001 gttgccacgg atgaagcgct cgacgatcac ctcgctgcct tccgcgatgg ccagatgata
     6061 ggccgcctcg atttcggcgc gcgtcttcag gtcggtggag acgcctcggc catggttgcc
     6121 gtcgaccggc ttgatcacca ccggcaggcc gatgtccaag gcggactccc aggcatcttc
     6181 cggactcgcc acgatgtgtc cctcgggtac gggcacgccg caagactgca acagattctt
     6241 ggtcagatcc ttgtcgcgcg agatggtctc gccgatcgcg ctggtcagat cggtctcggc
     6301 cgtccagatg cggcggctac gaacgccctg gcccagttgt accagattgc cttcggtcag
     6361 tctgttggag gggatgcgtc gcgcgcttgc cgcatccacg atgcaggcgg tgctggggcc
     6421 caggcatagc gagtccacca tgtcgcgcag gttggcgacg gtggctgaca catcgtaggg
     6481 acggtcttcc atcgcggcca gcaccagttc gcgcgccgcc agaaatgcgg tgtggctgac
     6541 ctgttcgtga cgggagcgta tggcgacttt atagatgccg cgtgcggaag tttcgcgcgc
     6601 cttgccgaag ccgctttgca tgccggccag attttgcaac tcgagggtga catgttccag
     6661 aacatgagcg gcccaggtac cttcgcgcaa gcgttgcagg aagccgccgc gttcgccgat
     6721 gccgcaacgg tgttcgatca gcgtgggcag ccaggatgac aggcgctcgt aaaagccggg
     6781 aatggtgttg gagggggcgt cttccagtgt gccgatatcg acccaggctt ccagtaccgg
     6841 acgataagtc cagatattcg ggccgcgcag gtgagtgatg cgcaggattt ttatatcgta
     6901 tttccccccg gcttgggact gggaggagtg tggcaggggt tcgacggcgc tggtgttcaa
     6961 ggaaacgcac ccaaaagtta tccggttggg cgagcattct cgcacgagaa tcacctctgt
     7021 caccagtgcg tcgcgcactc ttttttttgg cctctcggtt atactcgcgc cgcccgtaac
     7081 ccactccccg gaccgtacct cggcagacac agcggatgaa cacgactact tcatatttac
     7141 ccgactcttc cttgccaccc gtgctgcgcg ccaccccctc gttgcaggat ggggagcatg
     7201 cgctggcctg gctggacacc gatctggatg ccgccttgcg ttttgcgccc ggcatcgtgc
     7261 tggttaccga gcggcgcatt ctggccctgc cagccggcgc ggtcgattgg caggattggt
     7321 cttatcgtgc cggattggtg ttgcagcatc acgaccacgc cggggttggg acgctggagc
     7381 tgctggacgg gcagggttgc ctcgcccgct ggcgttacac gctgggcgag aatctagcgg
     7441 cattgcggct gatcgcccag ttcgagcgca ggctttcgag tcacctgagc ggacagcctg
     7501 ttgccgagga gacgcaggcg gtctgtccca agtgcaagac ggcgctacct tccggcgagg
     7561 atgaatgccc gatctgcgcg cgcacgatcg acgcgccgcc ctcgacctgg acgctgttgc
     7621 gtctgtggcg tttcgcaaaa ccctaccgta accagttgct ggcaggcttc ctgctcacgc
     7681 tggccgctac ggctgcgaca ctggtgccgc cttatctggt catgccgctg atggacaagg
     7741 tgctgatccc ctatcagaac ggcaaaccga tcgacaccgg gctggtcacg ctgctgctgg
     7801 ccgggctgtt cggttccgcg ct
//
LOCUS       DASUIV010000122         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1053013, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000122 DASUIV010000000
VERSION     DASUIV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053013"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..872)
                     /locus_tag="VFW59_04605"
     CDS             complement(183..872)
                     /locus_tag="VFW59_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363526.1"
                     /translation="MSRNFVEISDGQKDNAPENNGQAELTKQLAGKLQELNEQTAPLP
                     RARLQLDVAELLVALERKADAWTLAREAFATALAQDAWKDAVEACDVLYQCEQQGSVA
                     ALGMGVWLAVSFPVDPELTLAMLMHVVDETPDHADGAALAAVAARYVIDLRADDAQHE
                     NLGLFANHLIAQVAARHSKVRDQAALDQWMDRLELRDPQVFLPRLGRVVDIIVGDDWW
                     FDRDELRKKFD"
     gene            complement(1340..1708)
                     /locus_tag="VFW59_04610"
     CDS             complement(1340..1708)
                     /locus_tag="VFW59_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363527.1"
                     /translation="MNEHDAPGAPDRGGCVALIYALAAERLSVHYEHGQWLNSSQGAT
                     LAADWLSRSKRSLPLAERRRLSDLSDRLARQIASTTSRDAGLYIAHEMQESLDPRYQS
                     EVGLAIMAECESLFDEEPPA"
     gene            1844..2248
                     /locus_tag="VFW59_04615"
     CDS             1844..2248
                     /locus_tag="VFW59_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX5363528.1"
                     /translation="MQKPINIPVVSADMQQGDKSSCDGNEIVALMVLGDSMEPEFVEG
                     EILVIEMGRPAMAGAYVIGWANEEHIFRQLGRDEEGGWQLHALNPAYPAIAISGLDQI
                     KGVITHKKKPGSRKSVKYYDEQADGCCPDSPR"
     gene            2292..2585
                     /locus_tag="VFW59_04620"
     CDS             2292..2585
                     /locus_tag="VFW59_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEX5363529.1"
                     /translation="MRVIAISTLREFWEKHPQAETPLRSWYADACYADWKTPSDIKAA
                     HRKASFVGNNRIVFNIKGNDYRLIVAVHYNRGMLYIRFVGTHAEYNAINAGKV"
     gene            2588..2962
                     /locus_tag="VFW59_04625"
     CDS             2588..2962
                     /locus_tag="VFW59_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5363530.1"
                     /translation="MELKPIRTKAEYKAALREIEALFDVPEKTPEADRLEVLVMLVEK
                     YEAQHFPIPAPDPIDFLNYAMETRGLTRKDLEAYIGSRGRVAEVLNRARPLTLAMIRR
                     LSEGLKLPADVLIADYELRHAA"
     gene            complement(3062..>4081)
                     /locus_tag="VFW59_04630"
     CDS             complement(3062..>4081)
                     /locus_tag="VFW59_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5363531.1"
                     /translation="EQIARAASRPVLILSFANGISGQLENAMQDRGVEDKVITSTFHS
                     WCWKMLRTYGLPIPTEKDIPDFTERQAANVQAVLDAAESGLIPGGQYDAVLIDEAHDF
                     EPQWLALAAKMVNPDTKALMIVYDDAQAIYKGRKRPVWKQLGIEAAGRTTVLKVNYRN
                     TAQILRFARKFAADVIGAPGVCAGDESAILMPEDGGREGVEPVVRQCVSYDGEAHAIA
                     EWLLGRKAAGYQWGQMAVLYPRHFIGERFEKILTKSGVPVDVAKEHGNRIETGRDAVR
                     FLSMHNAKGLEFPCVAIGGLGELKKLEDIEDDIRLTYVAITRATHEAFITYSNMSELV
                     GRLVV"
BASE COUNT          765 a         1264 c         1205 g          847 t
ORIGIN      
        1 aggtcgccag aattaacctg aaaacctttt cttatccacg aaaaacacga aagtcacaaa
       61 ataaaacaat aagttgctaa ttctttgcaa ttcgcctgtc cggtgatgcc agcaatcagt
      121 atttgctttt gaattttttc gtgtttcttc gtgtctttcg tggacaactg ctttttcttg
      181 tatcagtcga atttcttgcg cagctcgtcg cggtcgaacc accagtcgtc gccgacgatg
      241 atgtcgacca cccggcccag acgcggcagg aacacctgcg gatcgcgcag ttccagcctg
      301 tccatccact ggtcgagggc ggcctggtcg cgcaccttgc tgtggcgtgc ggccacctgc
      361 gcgatcaggt ggttggcgaa caggcccaga ttttcgtgct gcgcgtcgtc ggcgcgcagg
      421 tcgatcacat agcgcgcggc gacggcggcg agtgccgcgc cgtccgcatg atccggcgtt
      481 tcgtcgacga catgcatcag catcgccagc gtcagctcgg ggtcgaccgg gaagctgacc
      541 gccagccaca cgcccatgcc cagcgccgcg acggagccct gctgctcgca ctggtacagc
      601 acgtcgcagg cctccacggc atctttccag gcgtcttgcg cgagcgcggt agcgaaggcc
      661 tcgcgcgcca gcgtccaggc atcggctttg cgctccagcg cgaccagcag ctccgcgaca
      721 tcgagctgca ggcgcgcgcg cggcagcggc gcggtctgct cgttcagctc ctgcagcttg
      781 ccggccagct gtttcgtcag ctcggcctgc ccgttgtttt ccggggcgtt gtctttctgg
      841 ccgtcgctga tctcgacgaa attcctgctc atacgtgtcc tgccatcctc atgtttcgtc
      901 attccggcgc aggccggaat ccagtctttt taatgtgaaa ccacactcgt gggagctccg
      961 gttttcgtag gagcggcttt agccgcgaag gtggccagga gtcgcaacct gtccatcgcg
     1021 actgaagtcg ctcccacagg gcgttttatc attcggggtg gcctcaatca ctatggttgt
     1081 taactgggct cccgctattt cgggagcgac ggaaattgta ttttttgctc gccgctttct
     1141 actctccgga gggtttgacg aagctgatga cctgcgcatg ctcgccgtcc ttgtgagggt
     1201 ctggcctgat cgaaatcgag ttgctctgca gtgtccatgg ctctataccg gagtgaattg
     1261 agggaaacga ctctactgga atggctgcgc tgtcgctatg ccccgttcgg gcggtgtccg
     1321 ccccatcgac attaaggcgt cacgccggcg gctcttcatc gaacaggctt tcgcattcgg
     1381 ccatgatcgc cagcccgact tcggactggt agcgcgggtc cagcgattcc tgcatctcgt
     1441 gggcgatata caggcccgcg tcgcgcgagg tggtcgaggc gatctgccgg gcgagccggt
     1501 cgctcaggtc ggacaggcgg cggcgttccg ccagcggcag gctgcgtttg ctgcgggaca
     1561 gccagtcggc ggcgagcgtg gcgccctgcg aggagttcag ccattgcccg tgctcgtaat
     1621 ggacggacag gcgttcggcc gccagcgcgt aaatcagcgc gacgcagccg ccgcgatccg
     1681 gcgcgcccgg ggcatcatgt tcgttcacgc ggctctccct gtatttttgt gcggcggcat
     1741 tgtaaggcgc aaatcgcctg gacgcccaat ccgtggcgca aggttattcg gcatcagccg
     1801 atttggcgct atcatccggg ccgttcaatc cggagttttt cccatgcaga agccgatcaa
     1861 catccccgtc gtttcagccg atatgcagca gggcgacaag agcagctgcg acggcaacga
     1921 gatcgtcgcg ctgatggtgc tgggcgacag catggagccg gagttcgtcg aaggcgagat
     1981 actggtgatc gagatggggc ggccagcgat ggcgggtgcc tacgtgatcg gctgggcgaa
     2041 cgaagagcac atcttccgcc agctcgggcg cgacgaagag ggcggctggc agctgcacgc
     2101 gctcaaccct gcctatcccg cgatcgccat ttcggggctg gaccagatca agggcgtcat
     2161 cacccacaag aaaaagcccg gctcgcgcaa gtcggtgaag tactacgacg agcaggctga
     2221 cggatgctgc ccagattctc ctcggtaatt ccaaggttga acccaaaatg ggagcgtgct
     2281 agagtgacgc catgcgagtc atagccatca gcacgttgcg ggaattctgg gaaaagcacc
     2341 cgcaggcaga aacgccgctg cgttcatggt atgccgacgc atgctatgcg gactggaaaa
     2401 ctccgtcgga catcaaggcg gctcatcgta aggccagctt cgttggaaac aatcgcatcg
     2461 tgttcaatat caaaggcaac gattaccgcc tgatcgtggc cgtgcattac aaccggggca
     2521 tgctgtacat ccgctttgtc ggaacccatg ccgaatacaa cgcgataaac gcaggaaagg
     2581 tatagcaatg gaactcaaac cgattcggac caaagcagaa tacaaagccg ccttgcgcga
     2641 gatcgaggca ttgttcgatg tgcccgaaaa aacacccgag gcggaccggc tggaagtgct
     2701 cgtcatgctg gtggagaaat acgaagccca gcacttcccc attcccgccc ccgatcctat
     2761 cgatttcctg aactacgcga tggaaacgcg cgggctgacg cgcaaggatt tggaagcgta
     2821 catcggcagc cgtggccgag tggccgaagt gctgaaccgc gcgcgcccgc tgacactggc
     2881 gatgatccgg cgtttgtcgg aaggactcaa actacccgcc gatgtgctga tcgcggatta
     2941 tgaactgcgg cacgccgcat aattctgctg aagcggaagc attttgtagg atgggttgcc
     3001 cccattcggg gacgagagcg aagcgatacc catcattttt cacatgtgcg atgggtttca
     3061 cttacaccac cagccgcccg accagctccg acatgttcga ataagtgatg aatgcctcgt
     3121 gcgtcgcgcg cgtgatggcg acgtaggtca ggcggatgtc gtcttcgatg tcttcaagct
     3181 tcttcagctc gcccaatccg cctatcgcca cgcaggggaa ttccagcccc ttggcgttgt
     3241 gcatggagag aaatctcacg gcgtcccgtc cggtctcaat gcggttgccg tgttccttcg
     3301 ctacatcaac cggcacgcca gattttgtca aaatcttctc gaagcgctcg ccgatgaaat
     3361 gccggggata cagcacagcc atctgccccc actgataacc ggcggccttg cgtccaagta
     3421 accattccgc aatcgcatgc gcttcgccgt cgtagcttac gcactggcgc accactggct
     3481 ccaccccctc gcgcccgccg tcttccggca tcaggatcgc gctctcgtca cccgcacaca
     3541 cgcccggtgc gccgatcacg tcggcggcga atttgcgcgc gaagcgcaat atctgcgcgg
     3601 tgttgcggta attcaccttg agcaccgtgg tgcgtccggc ggcctcgatg ccgagctgct
     3661 tccacaccgg gcgtttgcgc cccttgtaga tcgcctgcgc atcgtcgtaa acgatcatca
     3721 gcgccttcgt gtccgggttc accatcttcg ccgccagcgc cagccattgc ggctcgaagt
     3781 cgtgcgcctc gtcgatcagc actgcatcgt attgcccgcc gggaatcagc ccgctctcgg
     3841 cggcatcgag cacggcttgc acattggccg cctgccgctc cgtaaagtcg gggatgtctt
     3901 tttcggtggg tatcggcagg ccgtaagtgc gcaacatttt ccagcaccag gaatggaagg
     3961 tcgaggtgat caccttgtct tccacgcccc ggtcctgcat cgcgttctcc agctgccccg
     4021 agatgccgtt agcaaaactc agtatcagca ccggtctgct cgccgcgcgc gcgatctgct
     4081 c
//
LOCUS       DASUIV010000123         7842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_681471, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000123 DASUIV010000000
VERSION     DASUIV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681471"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="aceE"
                     /locus_tag="VFW59_04635"
     CDS             complement(<1..771)
                     /gene="aceE"
                     /locus_tag="VFW59_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294585.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX5363532.1"
                     /translation="MANNIPDMDPQETQEWLDALESVLEHEGADRAHYLISQLIDKAR
                     LGGTDMPISATTPYVNSIPLDKEERSTGNHELEHRIRALMRWNAMAIILNANRESSEL
                     GGHIASFASAATLYDVGFNHFFHGKTDKHGGDLIYFQGHSSPGVYARAYLEGRLSEEQ
                     LYKFRQEVDGDGLSSYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFMRYLQHRGLAST
                     DGRRVWAFLGDGETDEPESLGAISMAGREKLDNLIFVIN"
     gene            complement(803..1390)
                     /locus_tag="VFW59_04640"
     CDS             complement(803..1390)
                     /locus_tag="VFW59_04640"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX5363533.1"
                     /translation="MSQGLKQSIRKSIRSLRDALPVELHAAHSAAIAARLLQLPEYRR
                     AQAVLGYMNFGAEFGCDLWAARVLADGKRLALPKVNHHTNQLDLYWVEDFENQLAPGL
                     WGIREPLVERCQRLETLNEVEFALLPGVAFTREGARLGYGGGFYDKLLARMTSRPVLA
                     AAAFELQLVEQLPQETTDIKVEIIVTERETIVCAA"
     gene            complement(1396..2916)
                     /gene="ilvA"
                     /locus_tag="VFW59_04645"
     CDS             complement(1396..2916)
                     /gene="ilvA"
                     /locus_tag="VFW59_04645"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX5363534.1"
                     /translation="MMNGYLKRILNARVYDVAVETPLEVAPNLSARIGNTVLLKREDM
                     QPVFSFKLRGAYNKMAQLTPAQLKRGVIAASAGNHAQGVAMSAHRLGCRATIVMPTTT
                     PQVKIEAVKRFGQKSVEVVLHGDSYSDAYSHALALEKKLKLTFVHPFDDPDVIAGQGT
                     IAMEILRQHQGPIHAIFCAIGGGGLISGVAAYVKELRPDIKVIGVQTTDSDAMYQSLK
                     AKKRVTLNDVGLFSDGTAVKLVGEETFRLCRKYVDEIVLVDTDAVCAAIKDVFQDTRS
                     ILEPAGALAVAGAKLYAKREKLKGETLVTIPSGANMNFDRLRFVAERADIGEKREAIL
                     AVTIPEMPGSFRKFCALIGSRNITEFNYRFADPKAAQVFVGIQVRNDAETAKLISALE
                     KNKLSTLDLTDNEMAKLHLRHLVGGHAPEAKDEILFRFEFPERPGALMNFLNSMNHNW
                     NISLFHYRNHGADYGRVLVGMQVPHHDKKALKTFLDTLGYRYWDESGNPAYRLFLG"
     gene            3093..5417
                     /locus_tag="VFW59_04650"
     CDS             3093..5417
                     /locus_tag="VFW59_04650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5363535.1"
                     /translation="MFSRPLHPALAACLAAAIVFAGVLSIEKLERTRATQDQRLHAME
                     RVSVLRSKLESSISRAVASTSSMAALNYIHPDLTREQFVEMAQGIVTTNPAIHHIALI
                     RGHAVSELYPPAAHPPSIAADIQDEALLRAPFKRMLNSRTTIISTPTPGNAAFSVFIP
                     VLHHNSQAVSGAISAAILLDRLLEDAGLADIEQTLQISLRTRDTPGQSGTCFYGSAGL
                     FSDSPVLQSVHVPGGEWEIAAHPRGGWSTGSPTLVTIRILGGILCLLAAALAFLLVRH
                     LQRRTDSELRLSISEAQLKQRSAELIHQNAVLEMLTHNADLPDILEMLALLVEVHHPD
                     MLCSILLLDPDGLHLRHGAAPNLPDFYNQAIDGLPIGQGIGCCGTAAHTGKRVIVEDI
                     QTHPYWENYRALAEQARLRSCWSQPIKDSAERVLGTFAIYHHFPATPVPAEIMLIERY
                     AALAALAIERSHNAEAMRLHDTALNSAANAIVITDLEGCIVWANHAFSLLTGYEFEEA
                     IGHELTRLIDSGKQDAAFYTHMWETLLSGEIWHGESINRRKDGNLFYDETTITPVRNH
                     HGELTHFVAIKQDISRRKQDEEHLKNLAFYDPLTQLPNRRLLLDRLNQSIASCKRTAH
                     HSALIFIDLDNFKPLNDRYGHDVGDLLLAEAAQRIVKCVREADTVSRFGGDEFVVLLK
                     DLDADPATSTGQATSIAEKIRAALSTPYRLKLLQPMQGQDEIVHRCTSSIGIALFSDQ
                     KVCAEEVLKQADIAMYQSKEAGRDTIRLYVADDR"
     gene            5470..5853
                     /gene="apaG"
                     /locus_tag="VFW59_04655"
     CDS             5470..5853
                     /gene="apaG"
                     /locus_tag="VFW59_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX5363536.1"
                     /translation="MEKLNRPGFIIETQVSYLAGRSSESEQRFVFSYTITITNVSNAA
                     AQLISRHWIITDANNQVQEVKGQGVVGEQPVLKPNQSFEYTSGTALTTQVGTMRGSYR
                     MQAEDGSQFDIDIPQFVLSVPRVLH"
     gene            5872..7077
                     /locus_tag="VFW59_04660"
     CDS             5872..7077
                     /locus_tag="VFW59_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEX5363537.1"
                     /translation="MLPRISLVLLSLLAGCAIQPTPPVIVPPVSTVSVPPLAVSKWER
                     LPGWQDSDLQTSWIAFRQSCLALRNKPGWQEVCAHSDQLGTPDNAMLREFFEQGFTPY
                     QVSNPDGTSEGMITGYYEPKLLGSRVRTDRFRYPLYAAPDDLLTIDLGEVYPQLKGMR
                     LRGRLQDKRIVPYYTRADIDEGKAALHGRELFWVADAVDLFFLQIQGSGRIELPDGSL
                     IKVGYADQNGHPYVSIGRKLVDMGALKLEEASMQGIKRWAIDHPQELRTLLEQNPSYV
                     FFRELPNGLSAPLGALGVPLTGAYSIAIDPRTVPLGAPVFLATTYPNSSEPLQRLMLA
                     QDTGGAIKGAVRADFFWGFGDEAGAMAGKMKQKGEMWVLLPKGMEPPAPQAAPQPTPP
                     VCVQAKNGQ"
BASE COUNT         1509 a         2527 c         2282 g         1524 t
ORIGIN      
        1 gttgatcacg aagatcaggt tgtccagctt ctcgcgtccg gccatcgaaa tcgcaccgag
       61 ggattccggc tcgtcggtct cgccgtcgcc gaggaaggcc cacactctac ggccgtcggt
      121 gctcgccaga ccgcgatgtt gcagatagcg catgaaacgc gcctgataga tcgccatcaa
      181 gggacccaag cccatggaca cggtggggaa ctgccagaaa tccggcatca accagggatg
      241 cgggtaggag gacaggccgt cgccgtccac ttcctgacgg aatttgtaca gctgctcttc
      301 gctcaggcgg ccttcgaggt aagcgcgcgc atacacaccg ggcgatgagt gaccctggaa
      361 atagatcagg tcgccgccat gcttgtcggt cttgccgtgg aagaaatggt tgaagcccac
      421 gtcatacagg gttgccgccg aggcgaagct ggcgatgtgg ccgcccagct ccgaggactc
      481 gcggttcgcg ttcaggatga tggccatcgc attccagcgc atcagcgcgc ggatgcggtg
      541 ctccagctcg tgattgccgg tcgaacgctc ttccttgtcc agcggaatgc tgttgacata
      601 gggcgtggtc gcgctgatcg gcatatccgt gccgcccagc cgcgccttgt cgatcaactg
      661 gctgatcagg tagtgggcgc gatccgcacc ctcatgttcc agcacggact caagcgcatc
      721 cagccactct tgtgtctctt gtggatccat atccgggatg ttgtttgcca tctgtagctc
      781 tttccttgtt gcgattcaat tgttatgcgg cacagactat ggtttcccgt tcggtgacga
      841 taatctctac cttgatatct gttgtttcct gcggcaactg ctcgaccagc tgcaactcga
      901 atgccgccgc cgccagcacc ggacgggacg tcatgcgggc cagcagcttg tcgtaaaaac
      961 cgccgccgta gccgagcctc gccccctcgc gggtgaacgc cacccccggc aagagggcga
     1021 attctacctc atttagcgtt tccagcctct ggcagcgttc caccagcggc tcgcgtatgc
     1081 cccacaaccc cggcgccagt tgattctcga agtcttctac ccagtacaga tcgagctgat
     1141 tggtgtgatg attcaccttg ggcagtgcca gccgcttgcc gtccgccagc acccgcgccg
     1201 cccacaggtc gcagccgaac tccgcgccga aattcatgta gcccagcact gcctgcgccc
     1261 gccgatattc cggcagctgc aacaaacgcg ccgcgatcgc cgcgctgtgc gcggcatgca
     1321 gctcgacggg gagagcgtcg cgcagggagc gtatggactt gcgtatggac tgtttgagcc
     1381 cctgcgacat gtccgtcaac ccaaaaacaa cctgtacgca gggttgccgc tctcgtccca
     1441 gtaacggtaa ccgagggtgt cgaggaaggt cttgagcgcc ttcttgtcgt ggtgcggcac
     1501 ctgcatgccg accagcacgc ggccgtagtc cgcgccgtgg ttgcggtaat ggaacaggct
     1561 gatgttccag ttgtggttca tgctgttgag gaagttcatc agcgcgccgg gacgctcggg
     1621 gaactcgaag cggaacagga tttcatcctt ggcctcgggc gcatggccgc cgaccagatg
     1681 gcgcaggtgc agcttggcca tttcgttgtc ggtcaaatcc agcgtgctca gcttgttctt
     1741 ttccagcgcg gaaatcagct tcgcggtctc ggcatcgttg cgcacctgga tgccgacgaa
     1801 aacctgcgcc gccttgggat cggcgaaacg gtagttgaat tcggtgatgt tgcggctgcc
     1861 gatcagcgca cagaatttgc ggaagctgcc gggcatctcg gggatggtga cggcgaggat
     1921 ggcctcgcgc ttctcgccga tgtcggcacg ctcggcgacg aagcgcaggc ggtcgaagtt
     1981 catgttcgcg ccgctgggga tggtgaccag cgtctcgccc ttgagctttt cgcgcttggc
     2041 ataaagcttg gcgcccgcca cggcgagcgc accggccggt tccaggatgg aacgggtatc
     2101 ctgaaacaca tccttgatcg ccgcgcacac cgcatcggtg tcgaccagca cgatctcgtc
     2161 cacatatttc ctgcagaggc ggaaggtctc ttcgcccacc agcttcaccg cagtgccgtc
     2221 ggaaaacagg ccgacgtcgt tgagcgtgac gcgcttcttg gccttgagcg attggtacat
     2281 cgcgtccgaa tcggtggtct gcacaccgat gaccttgatg tcagggcgca gttccttgac
     2341 gtaggccgcc acgcccgaga tcagcccgcc gccgccgatg gcgcagaaga tcgcatggat
     2401 cggcccctga tgctggcgca ggatttccat cgcgatggtg ccctgcccgg caatcacatc
     2461 ggggtcgtcg aagggatgca cgaaggtcag cttgagtttt ttctccagcg ccagcgcatg
     2521 gctgtaggca tcgctgtaac tgtcgccgtg cagcacgacc tccaccgatt tctgcccgaa
     2581 gcgtttgacg gcttctatct tcacctgcgg cgtggtggtc ggcatcacga tggtggcgcg
     2641 gcaacccagc cggtgcgcgc tcatcgccac gccctgcgcg tggtttcctg ccgaagccgc
     2701 gatcactccg cgcttcagct gtgccggggt gagctgcgcc atcttgttgt aggcgccgcg
     2761 cagcttgaac gagaacaccg gctgcatgtc ctcgcgcttg agcagcacgg tgttgccgat
     2821 acgcgccgag aggttgggcg cgacttccag cggggtttcg accgcgacgt cgtagacgcg
     2881 cgcattgagg atacgtttga gataaccgtt catgatccgt ttgcttgaaa aatacccgtc
     2941 aagggtagca caaagcaccc cccgcccgac aggcagcagc ccccgtcgag cacgccctca
     3001 gcattctcct agggccattt cagggctagc ctagctcaaa aacatgcgct ttaacgtata
     3061 actattcgtg tccggttaag ccttgaatcg ctatgttctc ccgccccctg caccctgctc
     3121 tcgcagcctg tctggcagcc gccatcgtgt tcgctggcgt gctctccatc gaaaagctag
     3181 aacgcactcg ggccactcaa gaccaacgcc tgcatgccat ggaacgcgtg tcggtcctgc
     3241 gctcgaaact ggaatccagc ataagccgtg cggttgcttc gaccagcagc atggccgcgc
     3301 tcaattacat ccatcccgac ctcacccgcg agcagtttgt cgaaatggca caaggcattg
     3361 tcaccaccaa tccggcgata caccacatcg ccctgatacg cggccatgca gtcagcgagc
     3421 tatacccccc ggcggctcat ccgccgagca tcgctgcgga cattcaggac gaagccttgc
     3481 tacgggctcc cttcaagcgg atgctgaact cgcgcacgac catcatttcc acccccacac
     3541 ccggcaacgc cgcattcagc gtattcattc cggtgctgca ccacaactcg caagccgtca
     3601 gcggggcgat cagcgccgcg atactgctcg accggctgct ggaagacgcg gggctcgccg
     3661 atatcgaaca gacgctccag atctcattgc gcacccgcga tactcccggg caaagcggca
     3721 cttgcttcta cggctcggcc ggtctctttt ccgactctcc cgtactgcaa tccgtccatg
     3781 tgcccggcgg cgaatgggag atcgccgcgc atccgcgtgg cggctggtca accggctcgc
     3841 cgacgctggt tacgattcgg atactgggcg ggatcctgtg cctgctggcg gcggccctgg
     3901 ccttcctgct ggtcaggcat ctgcagcgcc gtaccgattc cgagctgcgt ttgagcatca
     3961 gcgaagccca gcttaaacag cgctccgccg agctgatcca tcagaatgcc gtgctggaga
     4021 tgctcaccca caatgccgat ctgcccgaca tcctcgaaat gctggccttg ctggtcgaag
     4081 tgcatcatcc ggacatgctg tgcagcatcc tgctgctcga tccggacggc ctgcacctgc
     4141 gacatggcgc ggccccgaat ctgcccgact tctacaatca ggcaatcgac ggcctgccca
     4201 tcgggcaggg tataggttgc tgcggcacgg ccgcccatac cggcaagcgc gttatcgtcg
     4261 aagacatcca gacccacccc tactgggaga actaccgcgc gcttgcagaa caagcccggc
     4321 tgcgctcctg ctggtcgcag ccgatcaagg attcggcgga gcgggtcctg ggcacgttcg
     4381 ccatttatca ccatttcccc gcgacgccgg tgcctgccga gatcatgctg atcgaacgct
     4441 atgcggcgct ggccgcactg gccatcgaac gcagccacaa tgccgaagcc atgcgcctgc
     4501 atgacaccgc gctgaactct gccgccaatg ccatcgtcat caccgatctg gagggatgca
     4561 tcgtctgggc caaccatgcc ttcagcctat tgacggggta cgaattcgag gaggccatcg
     4621 gtcacgaact cacccgcctg atcgactccg gcaagcagga tgccgctttc tatacccaca
     4681 tgtgggaaac gctgctctcc ggcgagatct ggcatggaga gtcgatcaac cgccgcaagg
     4741 acggcaacct gttctacgac gagacgacga tcaccccggt gcgcaaccat cacggcgagc
     4801 tgactcattt tgttgcgata aaacaggata tctcgcgccg caaacaggac gaggaacacc
     4861 tcaagaatct ggcgttctac gacccgctga cacaactgcc caatcgtcgt ctgctgctcg
     4921 accgtctcaa tcagtcgatc gcctcgtgca agcgcacggc tcaccacagc gcccttatct
     4981 tcatcgatct ggacaacttc aaaccgctca atgacagata cggtcatgac gtcggcgatc
     5041 tgttgctggc ggaagcggct caacgcatcg tcaaatgcgt gcgcgaggca gacaccgtgt
     5101 cccgcttcgg cggggacgag ttcgtggtct tgctcaagga tctggatgca gaccctgcca
     5161 catcgaccgg gcaggctacc agcatagccg aaaagatccg cgccgccctg tccacgcctt
     5221 accgcctgaa gctgctgcaa cccatgcaag gacaggatga gatcgttcat cgctgcacct
     5281 ccagcatcgg catcgcgctg ttctccgacc aaaaagtttg cgcggaggag gtgctgaaac
     5341 aggccgacat cgcgatgtac cagtccaagg aggccgggcg cgacacgata cgcctgtatg
     5401 tcgccgatga tcgctgatcc attcgcatcg gtattgcgac ccacctgaaa atcggttcaa
     5461 atggtcggca tggaaaaact caaccgcccc ggattcatca tagaaactca ggtgtcctac
     5521 ttggcggggc gttccagcga gtccgaacag cgtttcgtgt tctcctatac catcaccatc
     5581 accaacgtca gcaatgctgc cgcacagctc atcagccgcc actggatcat caccgacgca
     5641 aacaatcagg tgcaagaggt caagggccag ggcgtggtcg gagagcagcc cgtcctcaaa
     5701 cccaaccaga gcttcgagta caccagcggc accgcgctga ccacccaggt cggcaccatg
     5761 cgcggcagct accgtatgca ggccgaagac ggcagccagt tcgatatcga cattccgcaa
     5821 ttcgtgctta gcgttccgcg cgtgttgcat taaccccatc ttcgatcaat catgctcccc
     5881 agaatatcgc tcgtcctttt gtcgctgctc gccggttgcg ctatccagcc cacgcccccc
     5941 gtcatcgtcc cgcccgtcag caccgtctcc gtcccgccgc tggccgtgag caaatgggag
     6001 aggctgccgg gctggcagga tagcgatctg caaaccagct ggatcgcatt ccgccagagc
     6061 tgcctcgcgc taagaaacaa acccggctgg caggaggtct gcgcccacag cgatcaactc
     6121 ggcacacccg acaatgccat gctgcgcgaa ttcttcgagc agggctttac gccttatcag
     6181 gtgtccaatc cggacggcac gtcggagggc atgatcaccg gttactacga gcctaaactc
     6241 ctcggcagcc gcgtgcgcac cgaccgcttc cgctatccgc tgtatgccgc accggacgac
     6301 ctgctgacca tagatctcgg cgaggtctat ccacaattga agggcatgcg cctgcgcggc
     6361 cgcctgcagg acaaacgcat cgttccttac tacacccgcg ccgatatcga cgaaggcaag
     6421 gcggcgctgc acggacgcga actgttctgg gtcgccgatg cggtcgatct gttcttcctg
     6481 cagattcagg gctccggacg catcgagctg ccggacggct cgctgatcaa ggtcggctac
     6541 gccgaccaga acggccaccc ctatgtctcc atcggcagga agctggtcga catgggcgcg
     6601 ctcaaactcg aagaggcgtc gatgcagggc atcaagcgct gggccatcga tcatccgcaa
     6661 gaactgcgca cattgctgga gcagaacccc agctacgtgt ttttccgcga actgccgaac
     6721 gggctgtccg caccgctagg cgcgctgggc gtaccgctga ccggggctta cagcatcgcc
     6781 atcgacccgc gtaccgtgcc gctcggcgca ccggtatttc tggcgaccac ctaccccaac
     6841 agcagcgaac cgctgcaacg gctgatgctg gcgcaagaca ccggcggcgc gatcaaggga
     6901 gcggtgcgcg ccgacttctt ctggggcttc ggcgacgaag ccggagcgat ggcgggtaag
     6961 atgaagcaga aaggggagat gtgggtgctg ctgccgaagg ggatggaacc gcctgcgccg
     7021 caagccgcgc ctcagcctac gccgccggtt tgcgtgcagg cgaagaacgg gcagtagcag
     7081 ttggtcgggc tatgcccgac aacttcaaag acaaaaccgc cggtggcaga ccggcagctg
     7141 gttacctttc ttgtcttggc aggaaaggta accgaaagaa gccgcccccg gtttgccgcc
     7201 cgctgcgcgg gttccctcga tctgcctcaa acgtgcgggg ctgcgcaact cgcacgatcc
     7261 gctacgcggc cacgtgctca aacagtgctc gcccaacttc accgctcgtt cacgtctgag
     7321 cgaggcggcg cacaagggaa ggcaagtcaa aggcggtttt gtgtgcgctg tgcgcacgat
     7381 tgtttttttg ttggcgaggc gggctttggc ccgcctcgcg ggtttgggtt ttgaggtttt
     7441 gaattttctc ccctgtgcgc cgccgagtag cggaggctgg tcaggggtag tcggcgagga
     7501 ctgtctgagc gcgaagcgcg agttccgcag ccgcctgacc agtcgagcaa cgcagggaac
     7561 cgcgtagcgg cggcaaaccg ggggcgcctt cttttgggtt actttttctt ggcgactcaa
     7621 gaaaaagtga ctagctgtcg ggctaccccc gacggttttg attttgattg atgggtatcg
     7681 ctgcgctcaa cccatccttg tatctttctc tccgtggtgg ttagctacta ccacacgaag
     7741 cagaggcagc ttgaacccat tcttaaggct tgaccttgtg gcgtagatca agccctctgc
     7801 ttcacctctc gccagcaaaa acactgaacg gtagccaagg gg
//
LOCUS       DASUIV010000124         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_587685, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000124 DASUIV010000000
VERSION     DASUIV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587685"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFW59_04665"
     CDS             <1..339
                     /locus_tag="VFW59_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5363538.1"
                     /translation="KPLIGKVLVHGHCHQKAFGAMTAMGKVLGLVPGLQVEFIESSCC
                     GMAGSFGMEAEHYAVAMQMGELSLLPTVRAAAADTLLVANGTSCRHQIRDGAAREAQH
                     LAQVLQRALQ"
     gene            582..1442
                     /locus_tag="VFW59_04670"
     CDS             582..1442
                     /locus_tag="VFW59_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEX5363539.1"
                     /translation="MNTALENIFTGLSDGSVVPYLGPGALNGVIAPATGRAIPADSDS
                     LILAMNNGQPMAPKLMYEFPRAAMNVELKRGRSAVNKFLDATYRDTQWSTSALHSWLA
                     QQNLPYIIDTNRDTQLQLQYAATPHTLIVGVARISGKEFRFRIYHFDGTGYSPVEQEA
                     VDTRLPILFKPLGTPLPESNYIASDADFVDYITELMGGYAIPSFIKRMRQNKRYVLIG
                     QRLNRDTDRMVYSDVIFGADKPAGWALIPHPTDKEKRYLEKLNVEIVNADVADFLSAA
                     QSWNGSLAAA"
     gene            1439..1846
                     /locus_tag="VFW59_04675"
     CDS             1439..1846
                     /locus_tag="VFW59_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5363540.1"
                     /translation="MSSHPVVFDETRHALNVAQMDDTHREFMRQLASLIAASDAEFPV
                     LFQALINHTRVHFTEEGQLMRASKYRGLPEHESEHHRVLGELQQLNRSLKRGRLRLVR
                     AYVNEGLPEWFDSHLAMMDSALGMHLKKVGNGQ"
     gene            1947..2603
                     /locus_tag="VFW59_04680"
     CDS             1947..2603
                     /locus_tag="VFW59_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5363541.1"
                     /translation="MTHPIIQDLQWRYTTKKYDKTRKVPQADLDVLFEALRLSPSSIN
                     SQPWKFIVLGTDAAKERMNRTFANKFQFNQPHVFDCSHIILFAHNPHYTRANYAEVID
                     CAIADGRTKPEAREGAFGSFVFAEMNTDKTGDTSIWTKAQTYLALGNALHTLARLRID
                     STTMEGIDAELVNKEFAQELDGYHCNVALAIGYHHPQEDHNAKLPKSRRKLESILVQL
                     "
     gene            complement(2784..>3087)
                     /locus_tag="VFW59_04685"
     CDS             complement(2784..>3087)
                     /locus_tag="VFW59_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363542.1"
                     /translation="VAMTTKQLESDFSARLRDSIRQSHDLGYHPTRFEQMLDEQGALG
                     LARKLIKSGEIQDGFKAVVKLGRKDLTMESIMLEPQFKRLFSAGELEAATWRLGQA"
BASE COUNT          698 a          977 c          856 g          556 t
ORIGIN      
        1 aaaccgctga tcggaaaggt gctggtgcac ggccattgcc accagaaggc gttcggcgcg
       61 atgacggcga tgggcaaggt gctgggactg gtgccgggcc tgcaggtcga gttcatcgaa
      121 tcgagctgct gcggcatggc cggttcgttc ggcatggaag ccgagcatta cgcggtggct
      181 atgcagatgg gcgagctgtc cctgttgccg acggtgcgcg ctgccgccgc ggacacgctg
      241 ctggtcgcca acggcaccag ctgccgccac cagatccgcg acggcgcggc acgcgaagca
      301 caacatctgg cacaggtgct gcaacgcgca ttgcagtaga aaggggtcaa aggtaatcgc
      361 acgacaggcg tgacgctcac ctcgctgcca gcagcctgac gacaacgaac ctgaaccgcc
      421 ggaggcccta cccccgtagc cttgtcggac aagcacagcg gctttgtcgc acttgctcca
      481 aaacccgtca cgccacagcc ccgccaatat ggggattaga agctggcgcg gttattgcga
      541 gtcattcccc ggtcaaaact cttatccatc aaggagccgc aatgaatact gcattggaga
      601 acatctttac cggtctgtcg gatggcagcg tcgtccccta tctcggcccc ggcgcgctga
      661 acggcgtgat cgccccggcc acaggccgtg ccatccccgc cgacagcgac agcctgatcc
      721 tggcgatgaa caacggccag ccgatggcgc ccaagctcat gtacgaattc ccgcgcgcgg
      781 cgatgaacgt cgaactcaag cgcggccgtt cggcggtgaa caagttcctc gacgcgacct
      841 accgcgacac gcagtggagc acttccgcgc tgcacagctg gctcgcacag cagaacctgc
      901 cctacatcat cgacaccaac cgcgacacgc aactgcagct gcaatatgcc gccacgccgc
      961 acaccctgat cgtcggcgtc gcgcgcatct ccggcaagga gttccgcttc cgcatctacc
     1021 atttcgacgg caccggctac agccccgtcg agcaggaagc ggtcgacacc cgcctgccca
     1081 tcctgttcaa gccgctgggc acgccgctgc cagagtccaa ctacatcgcc tcggatgccg
     1141 acttcgtcga ctacatcacc gagctgatgg gcggctacgc catcccctcg ttcatcaaac
     1201 gcatgcgcca gaacaagcgc tacgtgctga tcggccagcg cctgaaccgc gacaccgacc
     1261 gcatggtgta ctccgacgtc atcttcggcg ccgacaagcc agccggctgg gcgctgatcc
     1321 cgcacccgac cgacaaggag aaacgctatc tggagaagct caacgtcgag atcgtcaatg
     1381 ccgacgtggc cgacttcctg agtgctgcgc aaagctggaa cggcagcctc gccgcggcat
     1441 gagcagccat ccggtagtct ttgacgagac acgccacgcg ctaaacgtgg cgcagatgga
     1501 cgacacccac cgggagttca tgcgccagct ggccagcctg atcgcggcca gcgatgcgga
     1561 gtttcccgtg ctatttcagg cgctcatcaa tcacacccgc gtccacttca ccgaggaagg
     1621 ccagctgatg cgcgcctcga aataccgcgg cctgcccgag cacgagagcg aacaccaccg
     1681 cgtgctgggc gaactgcaac aactcaaccg cagcctgaaa cgcggccgcc tgcggctggt
     1741 gcgcgcctat gtcaatgaag gcctgccgga gtggttcgac tcgcatctgg cgatgatgga
     1801 cagcgcgctg gggatgcatc tgaagaaggt ggggaacggg caataacttg gttattggca
     1861 agtgaggcac ggcacggtgc atacttgtgt aaataaaatc tgcagccata attgcgcccc
     1921 tcaccaacac tcgaaatgga gccgtcatga ctcaccccat cattcaggat ttgcagtggc
     1981 gctataccac caagaaatac gacaagacca gaaaagtgcc gcaggcagac ctcgatgttt
     2041 tgtttgaggc cctacgtctc tccccttctt cgatcaactc gcagccgtgg aaattcatcg
     2101 tcctcggcac agacgcagcc aaagagcgca tgaaccgcac ctttgccaac aagttccagt
     2161 tcaaccagcc gcatgtcttc gactgttcgc acatcattct gtttgcgcac aacccgcact
     2221 acacaagggc gaactatgcc gaagtgatcg actgcgccat tgcggacgga cgcaccaagc
     2281 ccgaagcgcg ggaaggcgct ttcggttcat tcgtatttgc cgaaatgaat acggacaaaa
     2341 ccggggacac cagcatctgg accaaggcgc aaacctatct ggcactcggc aatgccctgc
     2401 atacactggc gcggctcagg atcgactcca cgacgatgga agggatcgac gccgaactcg
     2461 tcaacaagga gtttgcgcaa gagctggatg gttatcactg caatgtcgcg ctggccatcg
     2521 gctatcacca cccgcaggaa gatcacaatg ccaagctgcc gaagtcgcgt cgcaagctgg
     2581 agagcatcct ggttcagttg tgaacgatag atcaaatcaa acctgagact tcctttggct
     2641 tcagcttagt cgaagccaaa ggtaaccagc tgccgggctg ccccggcaag gaggtgctga
     2701 ttgcctatct atgctgagct ggcagtgttg agaggcgagg cattaaaaag caattcgaag
     2761 ctgagccctt tagtttggat tcactatgcc tgccccagcc gccatgtagc tgcctcaagc
     2821 tcaccagcag agaagaggcg cttgaactgc ggttccagca tgattgactc catggttaaa
     2881 tcctttcgtc cgagcttgac tacagcctta aacccgtctt gaatttcgcc agacttgatc
     2941 agcttccgcg ccaacccaag cgcaccttgt tcgtcaagca tctgttcaaa acgagtcggg
     3001 tggtaaccta aatcatgtga ctgacgaata gagtctctca aacgcgcaga gaagtcggac
     3061 tctagttgct ttgtagtcat agcgact
//
LOCUS       DASUIV010000125         5933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_959258, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000125 DASUIV010000000
VERSION     DASUIV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959258"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1286)
                     /locus_tag="VFW59_04690"
     CDS             complement(189..1286)
                     /locus_tag="VFW59_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5363543.1"
                     /translation="MGDTGFGIQDSGYRGRGALAGILIFVSCFLLTACGKEPLYQEQG
                     YVFGTLVEVSVYGEDEARAKQAVSDVLHEFQRLHDMLHAWQPSPLSELNAAFAKGESK
                     AVAPELAAMLKDATRLAQQSQGLFNPAIGGLIQLWGFQSDEFKPLHPDEKLIAQWLAA
                     NPQMGDLVFLPSPQGGAELVSSSNKAVKLDLGGYAKGYALDRAAEILRKQGIHNALIN
                     IGGNVMALGQHGKRSWRVGIQHPRKSGALATLELHDGEAIGTSGDYQRYFMLGEQRYC
                     HLIDPRTGHPMQGVQAVTILTHGPNAGVESDGASKPLFISGAGGWRAAAQRMNLPEAM
                     LVDGQGNIHITAALQKRLEFTDKAIHPQVEP"
     gene            complement(1298..2566)
                     /gene="gshA"
                     /locus_tag="VFW59_04695"
     CDS             complement(1298..2566)
                     /gene="gshA"
                     /locus_tag="VFW59_04695"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX5363544.1"
                     /translation="MVPHLTTALTGPLFDLEQRLLCAQPSIEHWFRTQWLEHAAPFYA
                     SVDLRNSGFKLAPVDTNLFPGGFNNLNPDFLPLCVQAMQSAVEKICPEARGILLIPEN
                     HTRNLFYLQNVAQIVTILKQAGMRVRVGSLLPEITQPTAIQLPNGSALTLEPLVRKDN
                     RLGLADFDPCVVLLNNDLSAGVPEILKNLEQAVFPPLSAGWYMRLKSQHFAAYDRVAK
                     EFAALVGIDPWLINPYFATCNQVNFQERVGEECLAAQVDGVLQKMRSKYAEYGVKHDP
                     FVIVKADAGTYGMGIMTVKDASEITGLNRKQRNKMAVVKEGLQVHDVLVQEGVYTFEH
                     INDAVAEPVVYMVDHYVVGGFYRVHTGRGIDENLNAPGMSFEPLAFESCCTLPNPDCA
                     PDDTPNRFYAYGVVARLALLAASLELEAAE"
     gene            complement(2625..3491)
                     /gene="motB"
                     /locus_tag="VFW59_04700"
     CDS             complement(2625..3491)
                     /gene="motB"
                     /locus_tag="VFW59_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX5363545.1"
                     /translation="MAESNTKKVEGKGKRQIIVRRLRKKIPVTPHGAWKIAYADFVTA
                     LMAFFLLMWLTSTISVAELRLISDYFKTPVTMAMLGGKSNELDVSVVKVDGGKDLMKK
                     EGTIKESPVDQKQAEQALRRQEIQRLELLKAQLEQIVESSPELSKFKNQLLIDLTSEG
                     LRIMILDEKNRPMFELGRSTPQPYTINILHAVGRALNEVPNKISMSGHTDAMPYSGGS
                     TNYSNWELSADRANASRRELVAGGMDPEKIKRVVGLSSSVLFDPANPYSPNNRRISII
                     VMNRQAEEAASQ"
     regulatory      complement(3562..3663)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3784..5382
                     /locus_tag="VFW59_04705"
     CDS             3784..5382
                     /locus_tag="VFW59_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEX5363546.1"
                     /translation="MLSTANITMQFGAKPLFENVSVKFGDGNRYGLIGANGCGKSTFM
                     KILGRDLVQTAGEVMLDKNERLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMSERDAI
                     YANPDASEEDYMRAADLEATFAEYDGYTAEARAGELLLGVGIAIELHNGPMREVAPGF
                     KLRVLLAQALFSNPDILLLDEPTNNLDINTIRWLEDILNARTCTMVIISHDRHFLNSV
                     CTHMSDLDYGKITTYPGNYDDYMLASTQAREQQAADNARAKEKVAELKAFVARFSANA
                     SKAKQATSRARQIDKIKIEDIKPSSRQYPFIRFEYDEREKLHRTAVEVESLTKGFDKV
                     LFSNVNFRIDAGEKVAIIGPNGIGKTTLLRCLAGDLAPDYGQIKWTDKAKLGYYAQDH
                     SADFAEDKSMTEWMTYWRGPHDDDQAVRATLGRLLFSGDQALKQVKVLSGGEKGRMLF
                     GKLMLQRPNVLLMDEPTNHMDMESIESLNTALLEYKGTLVFVSHDREFVSSLATRIIE
                     ISDKGVNDYHGTYEEYLRDQGMAL"
     gene            5560..5841
                     /locus_tag="VFW59_04710"
     CDS             5560..5841
                     /locus_tag="VFW59_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5363547.1"
                     /translation="MIQNFATAETELFYSTGKSRRLPPDILKRAAMRLTQLDAATRIE
                     DLRLPPSNRLEALAHDRAGQWSIRINDQWRVCFRFENGDALDVEIVDYH"
BASE COUNT         1196 a         1878 c         1684 g         1175 t
ORIGIN      
        1 tccggccaat accgacagca ccttgagatt cttcggcact tcgccgagca catgtttgac
       61 gcaatccacc agactgtcgc ccagcccgtt atgcgtcacc agcaagattc caaccactta
      121 tatctccccc ccgaatatgc cagcagcgcg gcttggctca tgtcccgtga tttcccgcac
      181 ggaaccgctt atggctcgac ctgcggatga atggccttgt cggtgaattc tagccgtttc
      241 tgcaacgccg ctgtgatatg gatgttgccc tgcccgtcca ccagcatcgc ctcgggcaga
      301 ttcatccgct gtgccgccgc gcgccagccg cccgcgccgg agatgaacaa cggcttggag
      361 gccccgtcgg actccacgcc ggcgttcggg ccgtgcgtca ggatagtcac cgcctgcacc
      421 ccttgcatgg ggtggccggt gcgcggatcg atcaggtggc aatagcgctg ctcgcccagc
      481 atgaaatagc gctggtagtc gcccgaggtg ccgatggcct cgccgtcgtg caactccagc
      541 gtcgccagcg cgcccgattt gcgcggatgc tggatgccga cgcgccatga gcgtttaccg
      601 tgctggccca gcgccatgac attgccgccg atgttgatca gcgcgttgtg gatgccctgt
      661 ttgcgcaata tctccgcagc gcgatccagc gcataccctt tggcatagcc gccgagatcg
      721 agcttcacgg ccttgttgct gctgctcacc aattccgccc ccccttgggg cgaggggaga
      781 aagaccaggt cgcccatctg cggattcgcc gccagccatt gcgcgatcag tttttcgtcc
      841 ggatgcagcg gcttgaattc gtcgctctga aacccccaca actggatcag tccgccaatg
      901 gcgggattga acaatccctg cgattgctgc gcgagacggg tcgcatcttt cagcatcgcg
      961 gcgagttccg gcgcgacggc tttgctctcg cctttggcga atgccgcatt caactcgctc
     1021 aatggactgg gctgccaggc gtgcagcatg tcgtgcagcc gctggaactc gtgcagcaca
     1081 tcgctgactg cctgcttcgc gcgcgcctcg tcctcgccgt acacgctcac ctcgaccaac
     1141 gtgccgaaca catagccctg ctcctgatac agcggctcct tgccgcaggc cgtcagcagg
     1201 aaacaggaaa cgaaaatcag gataccggca agcgcaccgc gccccctgta tcccgaatcc
     1261 tgtatcccga accctgtatc ccccatcctg agtcctgtca ctcggcggct tccagctcca
     1321 gcgacgcggc cagcaatgcc agccgcgcca ccacgccgta agcgtagaag cggttcggcg
     1381 tgtcgtccgg cgcgcaatcg ggattcggca gcgtgcagca ggactcgaac gccagcggct
     1441 cgaagctcat gcccggcgcg ttgaggtttt cgtcgatgcc gcgcccggtg tgcacgcggt
     1501 aaaaaccgcc gaccacgtag tgatcgacca tgtacaccac cggctcggcc accgcgtcgt
     1561 tgatgtgttc gaaggtatac acgccctcct gcaccagcac gtcatgcacc tgcaagcctt
     1621 ccttgaccac cgccatcttg ttgcgctgct tgcggttcag gccggtgatc tcgctggcat
     1681 ccttcaccgt cataatgccc atgccatagg ttccggcatc ggctttgacg atgacgaagg
     1741 ggtcgtgttt cacgccgtac tcggcatatt tcgagcgcat cttttgcagc acgccgtcca
     1801 cctgcgccgc gagacactct tcgccgacgc gctcctggaa attgacctga ttgcaggttg
     1861 cgaaatacgg attgatcagc cacggatcga tgccgaccag cgcggcaaat tctttcgcga
     1921 cgcggtcata ggcagcgaag tgctgcgact tgagccgcat ataccagccc gccgacaacg
     1981 gcggaaatac cgcctgttcg aggttcttca ggatctccgg cacgccggcg gacagatcgt
     2041 tgttgagcag caccacgcag ggatcaaagt ccgcgagccc gagccggttg tccttgcgca
     2101 ccagcggttc cagcgtcagc gcgctgccgt tgggcaactg gatcgccgtc ggctgcgtga
     2161 tttccggcag caggctgccg acccgcaccc gcatcccggc ctgcttgagt atcgtgacga
     2221 tctgcgcgac gttttgcaga tagaacaggt tgcgcgtgtg gttctcgggt atcagcaata
     2281 tcccgcgcgc ctccgggcag attttttcga ccgcgctctg catcgcctgc acgcacagcg
     2341 gcagaaaatc gggattgaga ttattgaagc cgcccgggaa cagattggta tccaccggcg
     2401 cgagtttgaa gccgctgttg cgcaaatcca ccgacgcgta gaacggtgcg gcatgctcca
     2461 gccactgcgt gcggaaccag tgctcgatgc tcggctgcgc gcacagcaaa cgctgctcca
     2521 gatcgaacag cgggccagtc aatgcggtag tgagatgggg aaccatggtg tgcctgtaaa
     2581 attgattgca ggcattttag ccgatcaagc ggaacaattg ccccttactg gcttgccgcc
     2641 tcttccgcct ggcggttcat cacgatgatg ctgatgcggc gattgttcgg gctataggga
     2701 ttggcgggat cgaacagcac cgaagaggac aggccgacga cgcgtttgat tttttcgggg
     2761 tccatgccgc ccgcgaccag ctcgcgccgc gaggcattgg cgcgatccgc cgaaagctcc
     2821 cagttgctgt aattcgtgct gcctcccgaa tacggcatcg catcggtatg cccggacatg
     2881 ctgatcttgt tgggcacttc gttcagcgcg cggccgaccg catgcaggat attgatggta
     2941 tagggctgcg gcgtactccg gcccagctcg aacatcgggc ggtttttctc gtcgagaatc
     3001 atgatccgca gaccttcgct ggttaagtcg atcagcaact gattcttgaa tttgctcagt
     3061 tcgggactgc tctcgactat ctgctccaac tgggccttga gcaactcgag ccgctgtatt
     3121 tcctggcggc gcaaggcttg ttcggcttgc ttctgatcga caggactctc cttgatcgtg
     3181 ccctcttttt tcatcaagtc cttgccgccg tctaccttga cgacgctgac gtccagctcg
     3241 ttgctcttgc cgcccagcat ggccatggtc accggtgtct tgaagtagtc ggaaatgagc
     3301 cgcagctccg caactgaaat tgtcgaggtc agccacatca gcaaaaagaa agccatcagc
     3361 gcggtgacga aatcggcata cgcaatcttc caggccccat gcggggtgac cgggattttc
     3421 ttgcgcagcc tccggacaat gatttggcgc ttgcccttgc cttcgacttt ctttgtattg
     3481 gactcagcca tagaacctta ttgaaggttt atgcaaagaa tccggggacc ggtcgggaag
     3541 ctcaggatgg atgcgggatc acctggcgac cccgctggca ctcgactgct gtcgaatagg
     3601 aactcgtccc ttagcccgga agctttgcgt caccgccttt cgacgggttt gcccttgcag
     3661 gcgttgatgc atgaatcaac gatgcttccg cactctaatt gaaggcctcc gtccgcgtca
     3721 aacgaaaccg tgagaacggg gcttcatggt ggtaaaatgc gcgccttaat ttcaaggcac
     3781 accatgttat ctaccgcaaa catcaccatg caattcgggg ctaagcccct gttcgagaat
     3841 gtctccgtca aattcggcga cggcaaccgc tacggcctga tcggcgcgaa cggttgcggc
     3901 aagtccacat tcatgaagat cctcggccgc gatctggttc agaccgcggg tgaggtgatg
     3961 ctggacaaga acgagcgcct cggcaaattg cgccaggacc agttcgccta cgaagacatg
     4021 cgcgtgctgg acgtggtgat gatgggccat accgagatgt gggcggcgat gtcggaacgc
     4081 gacgcgatct atgcgaaccc ggatgcctcg gaagaagact acatgcgcgc cgccgatctg
     4141 gaggcgacct tcgccgagta cgacggttac accgccgagg cgcgtgccgg cgagctgctg
     4201 ctgggcgtag gcatcgcgat tgaactgcac aacggcccga tgcgcgaagt cgcacccggc
     4261 ttcaagctgc gcgtgctgct ggcgcaggca ctgttctcca acccggacat cctgctgctc
     4321 gacgaaccga ccaacaacct ggacatcaac accatccgct ggctggaaga catcctgaac
     4381 gcgcgtacct gcacgatggt catcatctcg cacgatcgcc acttcctgaa cagcgtgtgc
     4441 acgcacatgt ccgatctgga ctacggcaag atcaccacct accccggcaa ctacgacgac
     4501 tacatgctgg cctccacaca ggcgcgcgag caacaggcag ccgacaacgc ccgcgccaag
     4561 gagaaggtcg ccgaactgaa agccttcgtg gcacgcttct ccgccaatgc gtccaaggcc
     4621 aagcaggcca cctcgcgtgc gcgccagatc gacaagatca agatcgagga catcaagccg
     4681 tccagccgcc agtacccgtt catccgtttc gagtacgacg agcgcgaaaa gctgcaccgt
     4741 accgcagtgg aagtcgaatc gctcaccaag ggctttgaca aggtgctgtt ctcgaacgtg
     4801 aatttccgca tcgacgcggg cgagaaggtc gccatcatcg gcccgaacgg tatcggcaag
     4861 accacgctgc tgcgctgcct ggcgggcgac ctcgcaccgg actacggcca gatcaaatgg
     4921 accgacaagg ccaagctcgg ctactacgct caggaccaca gcgccgactt cgccgaagac
     4981 aagagcatga ccgagtggat gacctattgg cgcggcccgc acgacgacga tcaggccgtg
     5041 cgcgccacgc tgggccgttt gctgttctcc ggcgatcagg ccctcaaaca ggtgaaagtg
     5101 ctgtccggcg gcgagaaggg caggatgctg ttcggcaagc tgatgctgca aagacccaac
     5161 gtgctgctga tggacgagcc gaccaaccac atggacatgg aatccatcga gtcgctcaac
     5221 accgcactgc tcgaatacaa gggcacgctg gtgttcgtca gccacgaccg cgaattcgtg
     5281 tcgtcgctgg caacccgcat catcgagatc tccgacaagg gggtaaacga ttaccacggc
     5341 acgtacgaag agtatctgcg cgatcagggc atggcactgt aaatatccgg gcatccgtat
     5401 ccgttcccgg cctaatgctc gctgtaccag catgggtcac agcacatctt gcaatctgct
     5461 gttaactccc cttttccgca agctggcgag gggagttaag cctgcaccgc ccctccgata
     5521 ccgcttgacc gacataaact atttgtttat tgtccgccca tgatacaaaa cttcgctacc
     5581 gcagaaacgg agctgtttta ttccaccgga aaatcgcgcc gcctcccgcc tgatattctc
     5641 aagcgcgcgg caatgcgcct gacgcaactg gatgcggcga cacgtatcga agatttgcgt
     5701 ctgccgccat caaaccggct ggaagcattg gcgcacgacc gcgcaggtca gtggagcata
     5761 cgtatcaacg accagtggcg agtctgcttc cgctttgaaa atggcgatgc gctcgatgtc
     5821 gagatcgtcg attaccatta aggagaacca tcatggctaa aaaaggactg cccgcaatcc
     5881 atccgggcga atatctgggc gagaccctga acgagctgaa cctctcgcaa gcc
//
LOCUS       DASUIV010000126         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_216567, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000126 DASUIV010000000
VERSION     DASUIV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216567"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..545)
                     /locus_tag="VFW59_04715"
     CDS             complement(159..545)
                     /locus_tag="VFW59_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEX5363548.1"
                     /translation="MKSKSPACPCGAPDYAACCGRYHDGAAAPDAESLMRSRYSAYVL
                     KLEPYLLATWHPTTRPATLDLASDHTKWLGLEIKKFSPESADRAKVEFVARYKIGGRA
                     HRLHEVSRFLCEQGRWYYLDGEFEGE"
     gene            671..1924
                     /gene="hemA"
                     /locus_tag="VFW59_04720"
     CDS             671..1924
                     /gene="hemA"
                     /locus_tag="VFW59_04720"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX5363549.1"
                     /translation="MQLFAFGINHQTAPLAVREQVAFNADGMENALRDLVENGAAKEA
                     AILSTCNRTELYCNASSPEHAIDWLAQYHQLPRKDLDPYIYTLPRELAVKHSFRVASG
                     LDSMVLGEPQILGQMKQAVRQAEEAGTLGFLLHKLFQRTFSVAKDVRTQTEIGANLVS
                     MAAAAVKLAERIFPSIADQKILFIGAGEMIELNAVHFAARSPKHITVANRTLERAQTL
                     ARRINGQAITLTELPEQLAQHDIIITCTASQLPILGKGMVERALKARKHRPLFIVDLA
                     VPRDVEAEVSELNDVFLYTVDDIAEVVKDGLDARQSAVKEAEVIIDSGVSDFIHWMES
                     REVVPTIRALRDHAERNRRHEMEKAMRLLAKGESPEKVLEALSSSLTNKFLHAPTHAL
                     NQVHGDDREHFLEVIHRLYHLHTDE"
     gene            1921..3006
                     /gene="prfA"
                     /locus_tag="VFW59_04725"
     CDS             1921..3006
                     /gene="prfA"
                     /locus_tag="VFW59_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5363550.1"
                     /translation="MKPNIAAKLAQLSERLLEVDQLLSTEEATKDMDSFRRLSRERAE
                     LEPVVSLYHAYQTCLADIDTAKEMASDPEMKEFAEAEIKQGEETLTRLNSELQVQLLP
                     KDPNDERSVFLEVRAGTGGDEAALFAGDLFRMYSRFAERRRWQVEIISESPGEMGGYK
                     EIIAKIAGQGAYSVLKFESGAHRVQRVPATETQGRVHTSACTVAVMPEVDEVNDVVLN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGVVVECQDGRSQHQNKAQAMRVLAA
                     RIMDAQVRAQQADIAATRKSLVGSGDRSERIRTYNYPQGRVTDHRINLTLYKMDAIMD
                     GDISELTGALMAEHQAEQLAAMADDGI"
     gene            2996..>3375
                     /locus_tag="VFW59_04730"
     CDS             2996..>3375
                     /locus_tag="VFW59_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5363551.1"
                     /translation="MASSLAETLRIDSKRLEAALGLDSGTARIEVQMLLQATLQVNRA
                     YLLAHPEQVLNELQSATYGALFERRLNGEPIAYILGEREFFGLNFKVTPATLIPRPDT
                     ELLVELALQKIPQQGRVLDLGTGSG"
BASE COUNT          720 a         1120 c         1005 g          530 t
ORIGIN      
        1 caggtgctgg agctcatgga tcgacaatcg ccagaaacac cctctcccga cgacgagcag
       61 aacaaagacg aaccgtaggg gcacggcgcg ccgtgcccct acgaaaacct cctgccccat
      121 gcgggagcga ctttagtcgc gacaggcgca atgcgccctc actccccctc gaactccccg
      181 tccaggtaat accatcgccc ctgctcacac aagaagcggc tgacctcgtg caaacgatgg
      241 gcgcgtccac cgatcttgta gcgcgcaacg aactcgactt tggcgcgatc cgcagactcg
      301 ggcgagaatt tctttatttc cagccccagc cacttggtat ggtcgctcgc caagtcgagc
      361 gtagcgggtc gcgtcgtcgg atgccaggtc gcgagcagat aaggctccag cttgaggaca
      421 taggcgctgt agcgcgaacg cataagcgac tcggcgtcgg gcgcagcagc gccatcgtga
      481 taacgcccgc aacaggcggc ataatccggg gccccgcagg gacaggcagg cgatttcgat
      541 ttcatgcgtc attttaagcg actgcgcgac gcaagggcgc caaccaaggt aaaaatcccc
      601 acggatggca ttggtacgcg gttacgacta taattcgcca cctttttcat tggctctgcc
      661 gtataccaag atgcagctgt tcgcctttgg catcaatcat cagactgcgc cgcttgccgt
      721 gcgcgaacaa gtcgcgttca acgcagacgg gatggagaac gccctgcgcg atctggtgga
      781 gaacggtgcc gcgaaggaag cggcgatcct gtccacctgc aatcgcaccg aactgtattg
      841 caacgccagt tcacccgagc acgccatcga ctggctggcg cagtaccacc agctgccgcg
      901 caaggatctc gacccctaca tctacacctt gccacgcgaa ctggcggtga agcattcgtt
      961 ccgcgtcgcc agcggactgg actcgatggt gctgggcgag ccgcagattc tcggccagat
     1021 gaagcaagcc gtgcgtcagg ccgaagaagc cggcaccctg ggcttcctgc tgcacaagtt
     1081 gttccagcgc accttctcgg tcgccaagga tgtgcgcacc cagaccgaga tcggcgccaa
     1141 tctggtgtcg atggccgccg ccgcagtcaa gcttgccgaa cgcatcttcc ccagcatcgc
     1201 cgatcagaag atcctgttca tcggcgcagg cgagatgatc gaactcaatg cggtgcattt
     1261 cgcggcgcgt tcacccaaac acatcaccgt cgccaaccgc acgctggaac gcgcacagac
     1321 gctggcgcgc cgcatcaacg gtcaggccat cacgctgacc gagctgccgg agcaactggc
     1381 gcaacacgac atcatcatca cctgcaccgc cagccagctg ccgattctgg gcaagggcat
     1441 ggtggaacgc gcgctcaagg cacgcaaaca ccgcccgctg ttcatcgtcg atctggccgt
     1501 gccgcgcgac gtggaagcgg aagtatccga gctgaacgac gtgttcttgt acaccgtgga
     1561 cgacatcgcc gaagtggtga aagacggcct cgacgcgcgc cagagcgcgg tcaaggaagc
     1621 cgaggtcatc atcgactccg gcgtgtccga cttcatccac tggatggaat ctcgcgaagt
     1681 ggtgccgacc atccgcgcac tgcgcgacca tgccgagcgc aaccgccgcc atgagatgga
     1741 aaaggccatg cgcctgctgg ccaaaggcga gagcccggaa aaggtactgg aagccctgag
     1801 cagcagcctg accaacaaat tcctgcatgc gccgacccac gcgctgaatc aggtgcatgg
     1861 cgatgatcgc gagcatttcc tcgaagtcat ccaccgcctg taccacctgc ataccgacga
     1921 atgaaaccca acatcgccgc caaactcgct caactgagcg agcgcctgct tgaggtcgac
     1981 cagctgctga gcaccgaaga agccaccaaa gacatggatt cgttccgcag gctcagccgc
     2041 gagcgcgccg aactggagcc ggtggtgtcg ctgtatcacg cctaccagac ctgtctggcc
     2101 gacatcgata ctgcgaagga gatggcgagc gacccggaga tgaaagagtt cgccgaggcc
     2161 gagatcaagc agggcgaaga aacgctgacg cggctgaaca gcgaactgca ggtgcagctg
     2221 ctgccgaagg accccaacga cgagcgcagc gtgtttctcg aagtgcgcgc cggtaccggc
     2281 ggcgacgagg ccgcgctgtt cgctggcgac ctgttccgca tgtattcgcg cttcgccgaa
     2341 cgccgccgct ggcaggtcga gatcatctcc gaaagccccg gcgaaatggg cggatacaag
     2401 gagatcatcg ccaaaattgc cggacagggt gcctactcgg tgctcaaatt cgaatccggc
     2461 gcgcaccgcg tacagcgtgt gcccgccacc gagacacagg gccgcgtgca tacctcggcc
     2521 tgcaccgtcg cggtgatgcc ggaggtcgac gaggtcaacg acgtcgtgct gaaccccgcc
     2581 gatctgcgca tcgatacctt ccgcgcctct ggtgcgggcg gccagcacat caacaagacc
     2641 gactctgcgg tgcgcatcac ccacctgccg accggcgtgg tggtcgagtg ccaggacggc
     2701 cgctcgcagc accagaacaa ggctcaggcg atgcgcgtgc tggccgctcg catcatggat
     2761 gcgcaggtgc gcgcgcagca ggccgacatc gccgccacgc gcaagagcct ggtcggcagc
     2821 ggcgaccgct ccgagcgcat ccgcacctac aactacccgc agggccgcgt caccgaccac
     2881 cgcatcaacc tcacgctcta caagatggac gccatcatgg acggcgacat cagcgaactc
     2941 accggcgcgt tgatggctga gcatcaggcc gaacaactcg ccgccatggc ggacgatggc
     3001 atctagcctc gcagaaaccc tccgcatcga cagcaagcgg cttgaagcgg cactcggcct
     3061 ggactccggc acggcacgca tcgaggtgca gatgctgttg caggccacgc tgcaagtgaa
     3121 ccgcgcctat ctgctggctc acccggagca ggtgctgaac gagttgcagt ccgcaactta
     3181 cggagcccta ttcgagcgtc ggttaaacgg cgagccgatc gcctacatcc tcggcgagcg
     3241 agagttcttc gggctgaatt tcaaggtcac ccccgcgacg ctgatcccgc gccccgacac
     3301 cgagctgctg gtggagttgg cgttgcaaaa aatcccgcag caaggtcgcg tgctcgacct
     3361 cggcaccggc agcgg
//
LOCUS       DASUIV010000127        24635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_123502, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000127 DASUIV010000000
VERSION     DASUIV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 24635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24635
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123502"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1508
                     /locus_tag="VFW59_04735"
     CDS             279..1508
                     /locus_tag="VFW59_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX5363552.1"
                     /translation="MTVAKQALPAELIDSLLSGYKKPEDLIGENGLLKQLTKALVERA
                     LEAEMEAHLGHAKNEPVANPTGNTRNGKSRKTLKGEFGELPVEIPRDRHGSFEPQLIS
                     KHQTRWDGFDDKIISLYARGMTVREIQSHLEEMYGTEVSPTLISSVTDAVADEVKAWQ
                     SRPLDALYPIVYLDCIHVKVRDTGIVRAKAVYLALGINMSGEKELLGIWIAQTEGAKF
                     WLQVVTELKNRGVQDIFIACVDGLKGFPEAIETVFPKTDVQLCIVHLVRYSLNYVSWK
                     LRPEVAADLKAIYSAATVEDAELRLAEFEEKWGAAYPPIVQSWRRNWTRIIPFFDYPP
                     EIRRVIYTTNAIESVNMSLRKITKNRGSFPNDEALLKLFYLALNNISKRWTMPIRDWK
                     SALNRFTIQFEDRMPQQ"
     gene            complement(1602..2510)
                     /locus_tag="VFW59_04740"
     CDS             complement(1602..2510)
                     /locus_tag="VFW59_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5363553.1"
                     /translation="MDKAISNAVQLLEAHSDYKVVRRIVPRKVFSEDDGRALSRGVVV
                     DTETTGINPDKDAIIELGMVLFEFDPETGAAYRVLESFDQLEEPGFPIPPESTAVHGI
                     TDAMVAGKRIDDAEVARFLEGVSLVVAHNSKFDRVFLEKRLPLFESLPWGCSFAQVDW
                     SGEGIASAKLDYIAYQHGFFYEAHRAEGDCFALLEILQQRLPKSGELVLKSILNGLGQ
                     KSYKVYATGSPFETKDILKARGYRWDGDKKCWHHTLTGDEAVKAEVSWLKSDVYSGRS
                     AKVEIEVQNCLTRYSNRVGNRATREI"
     gene            complement(2543..4897)
                     /gene="parC"
                     /locus_tag="VFW59_04745"
     CDS             complement(2543..4897)
                     /gene="parC"
                     /locus_tag="VFW59_04745"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX5363554.1"
                     /translation="MTEQQVTEPTAGSGGGGNVRQFAPLPPPPEGDSVPLAQYAERAY
                     LEYAVSVVKGRALPDVCDGQKPVQRRILYAMREMGLAHNSKHVKSARVVGEVLGKFHP
                     HGDSSAYEAMVRLAQSFSMRYPLVDGQGNFGSRDGDNAAAMRYTETRLTPIADLLLSE
                     LSMGTVDMKPNYDGHFQEPAMLPARLPMVLLNGASGIAVGMATEIPSHNLREIGAAAA
                     VCVRNPACSDAELLASITGPDLPSGGQIISSPHEIRECYRTGRGSLKMRARWKIEEMA
                     RGQWRVAVYELPHGTSAKKIMEEIEELTNPKVKANKKALSQEQVQSKQLLLSVVDRVA
                     DESDKDHAVRLVFEPKSSRQSADELMTILLAHTSLESSLQLNMVMISLDGRPKQKPMA
                     EILREWGQFRLDTVRRRCEHRLGQVNDRIHILDGRMIVYLNLDEVIALIRESDDPKAA
                     LIERFSLSDRQAEDILEIRLRQLAKMEGIKIERELQQLKDEAGELNKLLGSDSAMRRK
                     VAKEIDDDIKTFGDDRRTLIEQAERVVLTTQVVDEPVTVMISRKHWGRTRQGHGLDLS
                     AISFKEGDGLLAAFECRTTDHCIVICSNGRVCSIPVHQLPGGRGDGVPLSTLIDVATG
                     AKIVHVICGNAEQHVLLSTSAGYGFTCTIGNMVGRNKAGKQFISVEDEQILPPALFTP
                     SEKSLVVAACRSGRLLAFLLVEMKQLSGGGKGVVVMGIAEGDELAAVAVISTPRVTVI
                     ANNGSREQLIQLSDKDLQSHFGKRARMGKALDMKSKSFVTQLKP"
     gene            complement(4971..6944)
                     /locus_tag="VFW59_04750"
     CDS             complement(4971..6944)
                     /locus_tag="VFW59_04750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX5363555.1"
                     /translation="MSVNYDESSFKVLRGLDPVRQRPGMYTTLDNPNHIITEVVDNAC
                     DEALAGFAKNIIVTAFADGSVSVQDDGRGIPVGLHPEEGVSVVEVAFTVLHSGGKFDK
                     EAGGAYKFAGGLHGVGVAVTNALSTAVEITVHRDGGEHYLRFDHGVALSPLVKMRDCP
                     KRKTGTTVRAWPDTKYFDAPNINMVQLERILRSKAVLLPGVTVTLHIEKTGETKAWSY
                     PDGLRGYLTEALNAQEEGGTLVAPIFAGERFIPKGGDDNFAEGEGASWALAWTEEGSI
                     TRESYVNLIPTWSGGTHDSGLRDAMYNAVKSFIELHGMMPKGVKLTSDDVSNRVSFVL
                     SAKVLDPSFQGQTKDRLVSRTALKLVSTMVTDPLLLWLNEHIDYGKRIAEIVIKQAQS
                     RMKSAQKVEKRKGSSVAVLPGKLTDAADFNPERNELFLVEGDSAGGSAKQGRNKEFQA
                     ILPLRGKVLNTFEVDRNRLFANAEVHDIAVAIGVDPHGVDEKADLSGLRYKGIYIMAD
                     ADVDGSHIATLLLTLFFRHFPRLVENGNIFLAQPPLFRIDVPAQGKKPIRKIYALNED
                     ELVSIEDRLRKDKVREGAWSISRFKGLGEMNPEQLWDTTMNPDTRRALCVALDAGSVD
                     ATERTMTMLMAKQESGSRRAWLEYHGNEVTGDV"
     gene            complement(6993..7730)
                     /locus_tag="VFW59_04755"
     CDS             complement(6993..7730)
                     /locus_tag="VFW59_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEX5363556.1"
                     /translation="MTQHSLSNLFTPLSQIELDELDQFLLSDATSDEVMQLDALDGYL
                     TAIVCGPVALKPHLWLPRVWGPSVRDEPVFETMAQAQRIFDLIVRHMNGIISGMQADP
                     DAHEPIFDRVGFEDDPREYLDGEMWAYGFMVGIGLSQQSWQPFLEAADGADMLRPVYL
                     LGADDVSIEEEALVETPEQREELTQRIPASVAAIYRFWQPFRPAVVEPALDATKQRDS
                     LKIGRNDPCSCGSGKKFKKCCAAAVLH"
     gene            complement(8068..8144)
                     /locus_tag="VFW59_04760"
     tRNA            complement(8068..8144)
                     /locus_tag="VFW59_04760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8108..8110),aa:Pro,seq:ggg)
     gene            complement(8188..8574)
                     /locus_tag="VFW59_04765"
     CDS             complement(8188..8574)
                     /locus_tag="VFW59_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5363557.1"
                     /translation="MEPHKPNSELPPIPAKRYFTIGEVSELCGVKAHVLRYWEQEFTQ
                     LKPVKRSGNRRYYQHHEVLLIRRIRELLYEQGFTISGARNRLESLAHGTSDDAVSSAS
                     AAVSSQNVDIAAMRREIRSIIELLEA"
     gene            complement(8555..8866)
                     /locus_tag="VFW59_04770"
     CDS             complement(8555..8866)
                     /locus_tag="VFW59_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX5363558.1"
                     /translation="MNSTLTKAELADLLFAKVGLNKREAKDMVEAFFEEIRAQLEKGE
                     SVKLSGFGNFQLRDKPQRPGRNPKTGEEIPITARRVVTFHPSQKLKTMVEKSYHGTPQ
                     A"
     gene            complement(8876..11233)
                     /gene="pheT"
                     /locus_tag="VFW59_04775"
     CDS             complement(8876..11233)
                     /gene="pheT"
                     /locus_tag="VFW59_04775"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5363559.1"
                     /translation="MKFSENWLRTWVNPALSSDELGHALTMAGLEVEALEAVAPPFNN
                     VVIAEVLEVVKHPNADRLNVCQVNVGEAQPLTIVCGAANVAVGVKVPCARIGAVLPGD
                     FKIKQAKVRSIESFGMLCSAVELGLAEASDGLWLLPADAPVGKTLREYLELDDQLFTL
                     KLTPNRSDCSGIAGLAREVAALTGSVLQPVAITEQPVALSEQLSVKVADTEACPLYCG
                     RLVRGVNAAAATPSWMLRRLERSGLRGINAVVDITNYVMMELGQPMHAFDVAQLSGGI
                     AVRRARKDEALTLLNEQSVTLDEDILVIADDARVLALAGIMGGQGSGVEITTQDVFLE
                     SAFFHPDAIAGRARRFGLATDSSFRFERGVDFAATRQALERATQLLLEICGGQAGSIC
                     EVRGELPARPAIALRRSRVTRVLGIELDNAQIAALLQGLQFEFTAKGDDFCVIPPSFR
                     FDLSIEADLIEEVARLYGYDNIPALAPQATLTMLPDSESQRSMEQIQQTLVARDYQEI
                     VSYAFLEEQVERELCGNAAPVALQNPIASNLAVMRSSLIGGLVGALRYNLNRKQSRVR
                     LFEAGACFAKVGDQYVQTQRLSAIAYGASLTEQWGASAKAVDFYDVKADVEALFAPQV
                     LRFVAATHPALHPGRSAQIYRGEQAIGWLGELHPKWQQQYDMPQAAIWFEVTVDALVQ
                     VQVPRMSEIAKFLPVRRDLAVLVDEAVDVQSLLDTMRNIAAPFVAEVALFDVYRGKGV
                     EQGKKSLAFRVLLQDTQKTLTDSEIEPSVALLVDALQKNGAQLRM"
     gene            complement(11244..12227)
                     /gene="pheS"
                     /locus_tag="VFW59_04780"
     CDS             complement(11244..12227)
                     /gene="pheS"
                     /locus_tag="VFW59_04780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5363560.1"
                     /translation="MEQLQQILDQALQQFSATSDEAELEQIKARYLGKEGSLTGLLKG
                     LGKLSAEERPAAGARINQVKQGIEAALQARRDALQQNKLEQKLAAESLDVTLPGRGLS
                     TGGLHPVTRTLQRIEQLFHSLGFATSSGPEIEHDFYNFTALNIPENHPARAMHDTFYI
                     DPQHVLRTHTSPVQVRYMEANQPPLKIISPGRVYRVDSDATHSPMFHQVEGLWVDEEV
                     SFANLKGVVQDFLQRFFERDDLRVRFRPSFFPFTEPSAEMDMSWNGGWLEIGGCGMVH
                     PNVLKHVNIDSEKYLGFAFGLGVERLAMLRYGVNDLRQFYESDLRFLKQFN"
     gene            complement(12299..12658)
                     /gene="rplT"
                     /locus_tag="VFW59_04785"
     CDS             complement(12299..12658)
                     /gene="rplT"
                     /locus_tag="VFW59_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5363561.1"
                     /translation="MPRVKRGVVARARHKKVLALAKGYRGRRKNVYRIAKQAVMKAGQ
                     YAYRDRRQKKRQFRTLWIARINAAAREQGMTYSVFMNGLKKAEINVDRKVLADIAVFD
                     KAAFAQFVVQAKASLGV"
     gene            complement(12669..12866)
                     /gene="rpmI"
                     /locus_tag="VFW59_04790"
     CDS             complement(12669..12866)
                     /gene="rpmI"
                     /locus_tag="VFW59_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5363562.1"
                     /translation="MPKMKTKSGAKKRFSVRAGGSIKRSQAFKRHILTKKTTKNKRQL
                     RGTTEVHATNTASVRAMMPYA"
     gene            complement(12999..13523)
                     /gene="infC"
                     /locus_tag="VFW59_04795"
     CDS             complement(12999..13523)
                     /gene="infC"
                     /locus_tag="VFW59_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5363563.1"
                     /translation="MAQDKSQRLNEMITAPQVRLIGVEKEPLGIVAIAEALRLADEAE
                     LDLVEISPLADPPVCRIMDFGKFKYAESKKQHEAKLKQKQVQIKEIKFRPGTDDGDYN
                     IKLRNLIKFLNEGDKTKITLRFRGREMAHQEIGMRLIERVRADLAEHGVVEQFPKMEG
                     RQMVMVLSPKVKTK"
     gene            complement(13548..15461)
                     /gene="thrS"
                     /locus_tag="VFW59_04800"
     CDS             complement(13548..15461)
                     /gene="thrS"
                     /locus_tag="VFW59_04800"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5363564.1"
                     /translation="MPVITLPDGSQRSFEHPVTVTDVAMSIGAGLAKAALAGKVDGKV
                     VDCSYLIEQDAALAIITGKDADGVEIIRHSTAHLLAHAVKELFPDAQVTIGPVIENGF
                     FYDFSYKRPFTPEDLAAIEKRMAELVKQDMPVTRRVLPRDEAVSYFKGIGEHYKAEII
                     ESIPADQDVSLYTEGGFTDLCRGPHVPSTGKLKVFKLMKLAGAYWRGDSNNEMLQRIY
                     GTAWTKKEELDAYLYQLEEAEKRDHRKLGRQLELFHMQDSSPGMVFWHPKGWTLWQEV
                     EQYMRRKFREHDYQEVRTPTIMDRSLWEKSGHWDNYRDNMFTTSSENRDYAVKPMNCP
                     GHVQIFNHGLHSYRDLPLRLAEFGSCHRNESSGSLHGLMRVRGFTQDDAHIFCTEDQV
                     QPEVSNFIVMLNEVYRDFGFSEVLVKLSTRPEKRVGSDETWDKAEAGLAAALQQNSLD
                     YEVQPGEGAFYGPKVEFTLKDSLGRLWQCGTIQLDFNLPTRLGAEFVDEDNGRKPPVM
                     LHRAILGSMERFIGILIEHHAGAFPLWLAPIQVVLMNISQAQEEYATEVAQALREAGL
                     RVQLDLRNEKITYKIREHSLQKLPYQLIVGDKEVAGRLIAVRTRSGEDLGQMTLEVLL
                     QRFKSEIKSGSAV"
     gene            complement(15511..15587)
                     /locus_tag="VFW59_04805"
     tRNA            complement(15511..15587)
                     /locus_tag="VFW59_04805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15551..15553),aa:Val,seq:gac)
     gene            complement(15662..17038)
                     /gene="dnaB"
                     /locus_tag="VFW59_04810"
     CDS             complement(15662..17038)
                     /gene="dnaB"
                     /locus_tag="VFW59_04810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5363565.1"
                     /translation="MNDFSNSDAQIDQIKLPPHSVEAEQSVLGGLLLESGALDRIIDL
                     VSDGDFYRHEHRLIFRQIVQLSEQAKPVDVITVAEALENNGELDKAGGLPYLGMLAQN
                     VPTAANVRRYGEIVRERSIMRKLVEVGSEIASSAYNPTGRDASQLLDEAERKVFEIAE
                     AGSKGKQGFFSMPPLLTQVVERIETLYGREDHSEVTGTATGYTDLDRMTSGLQAGDLV
                     IVAGRPSMGKTAFSINIAENVALDSGKPVAIFSMEMGASQLAMRMLGSVGKLNQHDLR
                     TGRLQDDDWGRLTHALGKLNDAKIFIDETAALSSLELRARSRRLHRQHGELGLIVVDY
                     LQLMSGNSGKASENRATEISEISRSLKGLAKELQVPVIALSQLNRSLEQRPNKRPVMS
                     DLRESGAIEQDADLILFIYRDEVYNNDSPDKGKAEIIIGKQRNGPIGTVALAFRGEYT
                     RFENLASY"
     gene            complement(17181..17630)
                     /gene="rplI"
                     /locus_tag="VFW59_04815"
     CDS             complement(17181..17630)
                     /gene="rplI"
                     /locus_tag="VFW59_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5363566.1"
                     /translation="MQIILMEKVVNVGQLGDVVKVKNGYARNFLIPQGKAKRATAANM
                     AEFEARRAEIEAADKAKLADAQVRGEKLAGLTVQISQKAGVDGKLFGSVTNADIAAAL
                     VAKGHAVEKSQVHMPEGHLKQIGDYSVNIGLHTDVVVGITVSVLGEH"
     gene            complement(17644..17919)
                     /gene="rpsR"
                     /locus_tag="VFW59_04820"
     CDS             complement(17644..17919)
                     /gene="rpsR"
                     /locus_tag="VFW59_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5363567.1"
                     /translation="MARPSDKKKDDKNKRSSRNNLFKRRKFCRFTVDKIAEVDYKDVN
                     ILKEFISENGKLIPARITGTKTRYQRQLSTAVKRARFLALLPYTDLH"
     gene            complement(17929..18225)
                     /gene="priB"
                     /locus_tag="VFW59_04825"
     CDS             complement(17929..18225)
                     /gene="priB"
                     /locus_tag="VFW59_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEX5363568.1"
                     /translation="MARNQVVISGELIALEGLRYTPAGVARVELTLRHSSQQPEANGE
                     RQVQCEVDALAFAEVAQKVGQFAIGQTVKVRGFLAQRGMRSRQLVLHINDIILE"
     gene            complement(18237..18623)
                     /gene="rpsF"
                     /locus_tag="VFW59_04830"
     CDS             complement(18237..18623)
                     /gene="rpsF"
                     /locus_tag="VFW59_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5363569.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMIERYRTLVTSKDGQIHRLEDWGR
                     RQLAYPIQKIHKAHYVLMNIECNQETLDELEHAFRFNDAVLRHLTVKTKAAVTEASAM
                     MKEEKSRSVPTEASAPAARAEEGAAA"
     gene            complement(18751..19530)
                     /locus_tag="VFW59_04835"
     CDS             complement(18751..19530)
                     /locus_tag="VFW59_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5363570.1"
                     /translation="MKLILSRDNPFFKDLVKLAGSARQRGKARQTLLDGTHLLTSYLD
                     AGEKPLHILLNSAALKDDEVEALLKRAAGVPMSQLDDKLFAELTELKTPTGILALVKI
                     PQPPVADHRFALLLEDIQDPGNLGSMLRTAAAAGCDAVYLSKGCADVWSPRVLRGAMG
                     GHFSLPIYEAEDLLAVAATFPGTIHAASLQATASLYDCDLRGDLALVIGNEGAGLSQA
                     LLDAVHRPFIIPMPGKVESLNAAAAAAVCLFEAARQRALPG"
     gene            complement(19554..20360)
                     /gene="scpB"
                     /locus_tag="VFW59_04840"
     CDS             complement(19554..20360)
                     /gene="scpB"
                     /locus_tag="VFW59_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5363571.1"
                     /translation="MSEPAEPSVQSEAASPVTGVAERSDDAIRVDLFGGKMNTVQFKI
                     VLETALLTSTEPLSVAELKKLSDVSLDTRQMEMLLESLAQDWQGRGVELSRVASGWRF
                     RAKPEMQPYLDRLNPQKPPRYSRAVLETLAIIAYHQPVTRGDIEEIRGVAVSSQILKT
                     LEARGWVEVVGTRDAPGKPELFATTKQLLDDLNLRGLQELPSLDEIGTLLDQETAAEN
                     ALAAAPVAPVAPPAAASEVAPSPVSAPAPSQPKIDPDAPEGWPFGPDDAE"
     gene            complement(20326..21132)
                     /locus_tag="VFW59_04845"
     CDS             complement(20326..21132)
                     /locus_tag="VFW59_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX5363572.1"
                     /translation="MSGTQLPLHPVARIHGEALLELPQDLYIPPDALELVLENFQGPL
                     DLLLYLIRKHNLDVLDIPMAELTRQYMSYIELMQQNRLELAAEYLLMAAVLIEIKSRM
                     LLPRPPKISDAEPEDPRAELMRRLLEYEQMKQAAQLLNALPQAGRDFEVVQVLIERTV
                     QERLPEVSVEDLRQTWMGLLARAKLNKHHKVRREQLSVREQMTHVLRCLKSGEYVPFE
                     HLFDIEGGVPKLVVTFIAVLELAKEYLVEIQQSETFGSIYVRTSRAISAE"
     gene            complement(21135..22337)
                     /locus_tag="VFW59_04850"
     CDS             complement(21135..22337)
                     /locus_tag="VFW59_04850"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5363573.1"
                     /translation="MFADRVLSGMRPTGSLHLGHYHGVLKNWVQMQHEFECLFFVADW
                     HALTTHYDTPQVIEQSVWDMVVDWLAAGVDPAHATLFIQSRVPEHAELHLLLSMITPL
                     GWLERMPTYKDQQEKLTSKDLSTYGFLGYPLLMSADVLIYRATQVPVGEDQVPHIEFT
                     RELARRFNHIYGREPGFEEKAEAAVKKLGSKKAKLYNELRVRFQEQGDDEALLSAKSL
                     LDEQQSLSHGDRERLFGYLEGGGKMILSEPQSMLTVASRMPGLDGQKMSKSYNNTITL
                     REDEASVGKKIRTMPTDPARIRRTDPGDPARCPVWQLHQVYSGEDTREWVQHGCKSAG
                     IGCIECKQPVIDAVLDEQRPMRERAQIYLDDPALVKNIIADGCEKARKLASETMRDVR
                     EAMGLSYS"
     gene            complement(22340..22987)
                     /locus_tag="VFW59_04855"
     CDS             complement(22340..22987)
                     /locus_tag="VFW59_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5363574.1"
                     /translation="MDIAHFVQLVSLAAIPVLFAITLHEAAHGYVARHFGDTTAYQQG
                     RISLNPVRHIDPVGTILLPLLTLLFGGIMFGWAKPVPVNFSALRRPKQDMLWVALAGP
                     ASNLVMALGWALIFKLTWLFPGNYFAEPLLGMAEIGIKINVVLMVLNLLPLPPLDGGR
                     VAVSLLPYRQAVQLSRIEPYGMFILIFMAVTPVLGKILSPLVGLVYKLISMLFGI"
     gene            complement(22987..23616)
                     /locus_tag="VFW59_04860"
     CDS             complement(22987..23616)
                     /locus_tag="VFW59_04860"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5363575.1"
                     /translation="MSQFFVIHPGNPQPRLIRQAAQMLRDGAIVVYPTDSCYALGCHL
                     DDKTAVTRIRQIRQLDEQHHLTLMCRDLSEISNYARVDNAKFRLLKANTPGSYTFILD
                     ATKEVPRRVQHPKRSTIGIRVPDHPVALALLEELGEPILSSTLILPEEEWPLNDVERI
                     RDLLEKQVDLVIDGGMVGVDFTTVIDLTGDTPVLLRRGKGDISPFGIAE"
     gene            complement(23616..24488)
                     /locus_tag="VFW59_04865"
     CDS             complement(23616..24488)
                     /locus_tag="VFW59_04865"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HEX5363576.1"
                     /translation="MLDYDLHCHSIISDGTLTPTELVARAAERGVKVLALTDHDNVDG
                     LDEARAAAAQCGMGFVNGVEISVSWRTHTLHIVGLNIDPHYAPLAEGLQTVRSGRGER
                     AQKMADELAKTGIGGTLQGAYQYAGNPNMIGRTHFARYLVEAGHCKDVKSVFNRYLAK
                     GKPGYVPHQWASLQDAIDWIRGSGGVAVLAHPGRYMVGRQSMGKKTMRELLVEFVECG
                     GQALEVVTGSHTPEQYAEFARYAEEFELMCSCGSDFHSPNESYRDMGRLPDFPLGCRP
                     VWESWGETFMQKVV"
     gene            complement(24537..>24635)
                     /locus_tag="VFW59_04870"
     CDS             complement(24537..>24635)
                     /locus_tag="VFW59_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX5363577.1"
                     /translation="IEIYRPLIADPKEVRRKRAEEGKVMKKGGGDA"
BASE COUNT         5073 a         7639 c         6760 g         5163 t
ORIGIN      
        1 tgcaccacat ccaaatctcc ccggaagata ttccttcaat tcgctcacag gaatcagtta
       61 ggcctacggc ttacggtgag atgactcgat atatttttga gtgtcttaag cttgcgatgg
      121 ggaaattttg ttcaacgaac gaggtagcct tattcattgc attccgttgc gcgccaaatc
      181 taactgacga tgaatataga atattccggg agggtgtaag aatttttgtg taaacggtca
      241 tttggcagga aactgccgcc cttacgaaag gagtcgtaat gaccgtagca aaacaagccc
      301 tcccggcaga gctgatcgac agcctgctgt ctggttacaa gaaacctgaa gacctgattg
      361 gcgagaacgg gctgctgaag cagctcacca aggcactagt cgaacgcgca ctggaagccg
      421 agatggaagc ccatctcggc catgccaaga acgagccggt cgccaacccc actggcaaca
      481 cccgcaacgg caagagccgg aagacgctca agggcgaatt cggcgaacta ccggttgaga
      541 ttccccgcga ccgccacggt agcttcgagc cacaattgat ttcgaagcac caaacccgct
      601 gggacggatt cgacgacaag atcatttcgc tgtatgcccg tggcatgacg gtgcgggaga
      661 ttcagagtca cctcgaagag atgtacggca ccgaggtctc gcccacactg atctcgtcgg
      721 tgaccgatgc cgtagccgac gaggtcaagg catggcagtc ccgaccgctg gatgcgctgt
      781 atcccatcgt ctatctggat tgcatccatg tgaaggtgcg cgataccggc atcgtccggg
      841 ccaaggcggt atacctggcg ctcggcatca acatgagtgg cgagaaagaa ttgctgggca
      901 tctggattgc ccagaccgag ggagcgaagt tctggctgca ggtggtgacc gagctgaaga
      961 accggggcgt gcaggacatc ttcatcgcct gcgtcgacgg attgaagggg ttcccggaag
     1021 ccatcgagac cgtgttcccc aagaccgacg tgcagctgtg catcgtccat ctggtgcgct
     1081 acagcctgaa ctacgtctcg tggaaactgc gtccggaagt cgctgccgac ctgaaggcca
     1141 tctacagcgc tgcgaccgtc gaggatgccg agttgcgact ggcagagttc gaggagaaat
     1201 ggggggccgc gtatccgccg atcgtccaat cctggcggcg caactggaca aggatcatcc
     1261 cgttcttcga ttacccgcca gagatacgtc gggtgatcta caccaccaat gccatcgagt
     1321 cggtgaacat gagcctgcgc aagatcacca agaatcgcgg ttcgttccct aacgatgagg
     1381 cgctgctgaa actgttctac ttggcgctga acaacatcag caagcggtgg acgatgccga
     1441 tccgtgattg gaagtctgcg ctaaaccggt ttaccatcca gttcgaggat cggatgcctc
     1501 agcaatagag aaaccccgtt tacacaaaat tcgggacacc ctcttatggg gtagccgaac
     1561 cagggtttgc aggtcagcaa aacttggccc agcctgaacc tctagatttc cctggtcgcc
     1621 ctattcccca cccgatttga atatcgggtc aggcaatttt gcacttcgat ctcaaccttg
     1681 gcactcctgc cgctgtaaac gtcgctcttc agccagctca cttcggcctt gaccgcttca
     1741 tcgccagtca aggtgtggtg ccagcatttc ttgtcgccat cccatcgata gccgcgtgcc
     1801 ttgaggatat cttttgtctc aaagggcgat ccggtggcat agaccttata gctcttctgc
     1861 cccagaccat tcagaataga cttcaagacc agctcgccgg acttggggag tctttgctgc
     1921 aggatttcga gcagcgcaaa acaatctccc tcggcccggt gtgcttcata aaaaaagccg
     1981 tgctggtagg cgatgtaatc gagcttggcc gaggcgatgc cctccccgct ccagtcgacc
     2041 tgcgcaaatg agcagcccca aggcagagac tcgaagagcg gcaagcgttt ctcgaggaag
     2101 acccggtcaa acttggaatt gtgcgccacg accagactca ccccttccag aaaacgcgcc
     2161 acttccgcgt catcgatacg cttgcccgca accatggcat cggtaatacc atgcacggcg
     2221 gtcgattcag ggggaatggg aaaacccggc tcttccagct gatcgaacga ttcgagtacc
     2281 cggtatgcgg cgcccgtttc cggatcgaac tcgaaaagca ccatacccaa ctcgatgatt
     2341 gcatctttat cgggattgat accggtagtt tccgtatcga ccaccacccc tctcgacaaa
     2401 gcccgtccgt cgtcttcgga aaacaccttg cgtggcacta tccttctgac gaccttgtaa
     2461 tccgagtgcg cctccaaaag ctgcactgca ttgctgatag ctttatccat ggatatgtcg
     2521 aatagtaagt ttcaggatcg gactatggtt tgagctgggt cacgaatgac ttgctcttca
     2581 tatccagcgc cttgcccata cgggcgcgtt tgccgaaatg gctttgcagg tctttatccg
     2641 acagctggat cagctgttcg cggctgccat tgttcgcaat gaccgtgact ctgggcgtgc
     2701 tgatcacagc gaccgcagcg agctcatcgc cctccgcgat ccccatcacc accacccctt
     2761 tgccgccacc cgaaagctgt ttcatctcaa ccagcaggaa agccagcaag cgaccggaac
     2821 ggcaggccgc cacgaccagc gatttctcgg atggcgtaaa caaggcaggc ggcagaatct
     2881 gttcgtcttc cacgctgatg aattgcttgc cggccttgtt gcgaccgacc atgttgccta
     2941 tcgtacaggt gaagccgtaa cccgccgaag tggacagcaa cacgtgctgt tccgcattac
     3001 cgcagatcac atgcacgatc tttgcacccg tcgccacgtc gatcagcgtt gacagcggta
     3061 cgccgtctcc ccgccctccc ggcaactggt gtaccgggat gctgcacacg cggccgttgc
     3121 tgcagatcac gatgcagtgg tcggtggtgc ggcactcgaa tgcggccagc aggccgtcgc
     3181 cttctttgaa cgaaatggcg gacaggtcca ggccgtggcc ttgtcttgtg cgtccccagt
     3241 gcttgcgcga aatcatcacc gtcaccggct catccacgac ctgagtggtc agcacgacgc
     3301 gctcggcctg ttcgatcagc gtgcggcggt cgtcaccgaa ggtcttgatg tcgtcgtcga
     3361 tctctttcgc cacttttctg cgcatcgcgg aatccgagcc cagcagtttg ttcagctcgc
     3421 cggcttcgtc tttcaactgt tgcagctcgc gctcgatctt gatgccttcc atcttggcca
     3481 actggcgcag gcgaatttcc aggatgtcct cggcttgccg gtcggacagc gagaagcgct
     3541 cgatcagcgc cgccttcgga tcatcggact cgcggatcag cgcgatcacc tcgtccaggt
     3601 tgagatagac gatcatgcgg ccatcgagga tgtggatgcg gtcattgacc tgtccgagcc
     3661 gatgctcgca gcgacggcgc acagtatcca gacggaactg cccccactcg cgcaggatct
     3721 cggccatcgg cttctgcttc gggcgaccgt ccagactgat catcaccatg ttgagctgca
     3781 gcgagctttc cagactggtg tgcgccagca ggatagtcat caactcatcg gcagattggc
     3841 ggctggattt cggttcgaac accagacgta ccgcgtgatc cttgtcagat tcgtcggcca
     3901 ccctgtccac cacggacaac agcagctgct tgctctgcac ctgctcctgc gacaaggcct
     3961 tcttgttggc cttgaccttc gggttcgtca gttcctcgat ctcctccatg atcttcttgg
     4021 cggaggttcc gtgcggcagt tcatacacgg ccacacgcca ctggccgcgc gccatctcct
     4081 cgatcttcca gcgcgcgcgc atcttcagcg aaccgcgtcc ggtgcgatag cattcgcgta
     4141 tctcgtgcgg cgacgaaatg atctgcccac cgctgggcag gtcggggccg gtgatcgaag
     4201 ccagcagctc tgcgtccgaa caagccgggt tgcgcacaca gaccgctgcc gccgcgccga
     4261 tctcgcgcag gttgtgcgac ggaatctcgg tcgccatgcc caccgcgatg ccggatgcgc
     4321 cgttgagcag caccatcggc aggcgcgccg gcagcatcgc aggttcctgg aaatgaccgt
     4381 cgtagttcgg cttcatgtcc accgtgccca tgctcagttc cgacagcagc agatcggcga
     4441 taggcgtcag ccgcgtttcc gtgtaacgca tcgccgccgc attgtcgccg tcgcgcgaac
     4501 cgaagttgcc ctgaccgtcg accagcggat agcgcatcga gaagctctgt gccagacgca
     4561 ccatcgcctc gtaggccgac gagtcgccgt gcgggtggaa cttacccagc acctcgccga
     4621 ccacgcgagc ggatttcacg tgcttgctgt tgtgcgccag tcccatctcg cgcatcgcat
     4681 agaggatgcg gcgctgtacc ggcttctgcc cgtcgcacac gtccggcaag gcacggccct
     4741 tgacgaccga caccgcatat tccagatagg cgcgctcggc gtactgcgcg agcggcacgg
     4801 agtcgccctc gggcggcggc gggagcggcg caaactggcg gacgttcccg ccaccacccg
     4861 agcccgcagt aggctccgtc acttgttgtt cagtcatttc ctgttcattt gcctgactag
     4921 ctacaggctg ttcgccctcg gcagtttcaa acaattctgg ttgcggaagg ttagacatca
     4981 cctgttacct catttccatg atattcgagc caggcgcgtc tggagcccga ctcctgtttt
     5041 gccatcagca tggtcattgt gcgttccgtt gcgtcgactg aaccggcatc cagtgccaca
     5101 cacagggcgc gccgggtgtc ggggttcatg gtcgtatccc ataactgctc cgggttcatc
     5161 tcgcccaaac ccttgaagcg cgagatggac caggcgcctt cgcgcacctt gtcttttctc
     5221 aggcggtctt ctatcgacac cagctcgtct tcattcaatg cgtagatctt gcgtatcggc
     5281 ttcttgccct gggctggaac atcgatgcgg aacagcggcg gttgagcgag gaagatattg
     5341 ccgttctcga ccagcctcgg gaaatgccgg aagaacagcg tcaacagcaa agtggcgata
     5401 tgcgagccgt ccacgtcggc atccgccatg atgtaaatgc ccttgtagcg cagtcccgac
     5461 agatcggcct tttcatccac cccatgcggg tcgaccccga tcgccacggc gatatcgtgc
     5521 acttccgcgt tggcgaacag ccggttgcgg tcgacctcga aggtgttgag caccttgccg
     5581 cgcagcggca gaatggcctg aaactccttg ttgcggccct gcttggcgga gccgcccgca
     5641 gagtccccct cgaccaggaa caactcgttg cgctccggat tgaagtccgc cgcatcggtc
     5701 agtttgccgg gcagcacggc aacggaagag cccttccgct tctcgacttt ttgcgccgat
     5761 ttcatgcggc tctgcgcctg cttgatcacg atctccgcga tgcgcttgcc gtaatcgata
     5821 tgctcgttca gccacagcag cagcgggtcg gtgaccatgg tggagaccag tttcagcgcg
     5881 gtgcgcgaaa ccagccggtc tttggtctgc ccctgaaagg aaggatcgag caccttggcg
     5941 gacagcacga aactgacgcg attggagacg tcgtccgagg tcagtttcac cccttttggc
     6001 atcatgccgt gcaactcgat gaagctcttc accgcgttgt acatcgcgtc gcgcagaccg
     6061 gaatcgtgcg tgccccccga ccaggtcgga atcaggttca cataggattc acgggtgata
     6121 gaaccttcct cggtccatgc cagcgcccac gatgcgcctt cgccctcggc gaagttgtcg
     6181 tcaccgccct tggggatgaa tcgctccccc gcgaagatag gcgcgaccag cgtgccgcct
     6241 tcctcctgcg cgttcaacgc ctcggtgaga taaccgcgca gcccatcggg ataggaccag
     6301 gccttggtct ctccggtctt ttcgatatgc agagtcaccg tcacgccggg cagcaacacc
     6361 gccttcgaac gcaggatgcg ctccagttgc accatgttga tgttgggcgc atcgaaatac
     6421 ttggtatccg gccaggcacg cacggtggtg ccggtcttgc gcttgggaca gtcgcgcatc
     6481 tttaccagcg gactcagtgc gactccgtga tcgaaacgca gataatgctc gccgccatcg
     6541 cggtgcaccg tgatctcgac ggcagtcgac agcgcattgg taaccgccac gccgacaccg
     6601 tgcaggccgc cggcaaattt gtacgcgcct ccggcctcct tgtcgaactt gcccccggag
     6661 tgcagcacgg taaaggccac ctcgacgacc gatacccctt cctccggatg gaggccgacc
     6721 gggatacccc ggccatcgtc ctgcacggac acggaaccat ccgcaaacgc ggtgacgatg
     6781 atgttcttgg cgaatccggc cagagcctcg tcgcaggcat tgtcgaccac ctcggtgatg
     6841 atgtgattgg gattatccag cgtcgtatac atgccgggac gctgccgtac gggatccagg
     6901 ccacgaagga ctttaaagga cgattcatcg taattaacgc tcaactgaca ctcctattgt
     6961 taaacgatat agcgaccgac ttgaagctgg cctcaatgta gcactgccgc tgcacaacac
     7021 ttcttgaatt tcttgccact cccacacgaa cagggatcat tgcgtccaat cttgagcgaa
     7081 tctcgttgct tcgtagcgtc gagagctggc tcaacgaccg caggccggaa tggctgccag
     7141 aaacggtaga tcgcagccac actggccggt atccgctggg tgagttcttc gcgctgctcc
     7201 ggcgtttcca ctagcgcctc ctcctcgatc gatacgtcat ctgcccccag caaatagaca
     7261 ggccgcagca tatcagcccc atcggcagct tccaagaatg gttgccagga ctgctggctc
     7321 aagccaatgc cgaccatgaa accatacgcc cacatctcac catcgagata ttctcgggga
     7381 tcatcctcga atcccacgcg atcaaagata ggctcatgcg catcgggatc ggcctgcata
     7441 cccgagatga tgccgttcat atgccggacg atcaaatcaa agatgcgctg ggcttgcgcc
     7501 atagtctcga acaccggctc gtccctgaca ctcggccccc agacgcgagg cagccatagg
     7561 tgaggcttca aggccacagg cccgcagaca atggcagtca gataaccatc cagtgcatcc
     7621 agctgcatga cctcgtcgga ggtcgcatcg gaaagaagga attgatcgag ttcgtcgagt
     7681 tcgatctggg acagcggagt gaacagatta ctgagagagt gttgagtcat agagcggcat
     7741 cgataaggaa gtgtatgtat tttactggtc actcgaacga gctaaatgct ttggcaaaca
     7801 atcccactca gccacatcaa aacacccact cttcggcctt gaaggaattt ttaacgctca
     7861 agcaacgccc caacaacccc atacgcacat attagagaac gaacgttctg ctgtcaaacg
     7921 atcatcaagc aacttcgcag gacagctacc tgccccatag atgactacga cgctatcgtt
     7981 attatttcgc gctacgaaga actagaacgc gtgactgaaa ttcagcacac ataaaaaaag
     8041 accagcaact aggctggtct ttatgtctgg tcggggcgag aggattcgaa cctccgacca
     8101 cttcaccccc agcgaagtgc gctaccaggc tgcgctacgc cccgagggcg cggattatag
     8161 cagagcgttg cgaatgatga agagttttta cgcttccagt aactcgataa tgctgcgaat
     8221 ttcacgacgc atagcggcga tatccacatt ttggctcgag actgcggctg atgcactcga
     8281 taccgcatca tcagaagtgc catgcgccaa actctccaga cgattgcgcg caccgctgat
     8341 ggtaaagccc tgttcgtaca acagttcacg gatgcgccgg atcaacaaca cctcatggtg
     8401 ctggtaatag cgccggttac cgctgcgctt gacaggtttg agctgggtga actcttgctc
     8461 ccagtaacgc aacacatgcg ccttcacccc gcataactcg ctgacttcac cgatggtgaa
     8521 atagcgtttg gcggggatag gaggaagttc ggaattaggc ttgtggggtt ccatgatagg
     8581 acttttccac catggtcttc agcttctggc tgggatgaaa ggtcaccacg cgacgcgcag
     8641 tgatgggaat ctcttcacca gttttcggat tgcggccagg acgttgtggc ttgtcgcgca
     8701 actggaaatt gccgaaaccg gaaagtttga cactctcgcc cttttccagc tgggcgcgaa
     8761 tctcctcgaa aaacgcttcc accatatcct tggcctcgcg cttgttgagc cccactttgg
     8821 cgaacaacaa atcggccagt tcggccttgg tcaacgtcga attcatcctc tccccttaca
     8881 tgcgcaattg cgcaccattc ttttgtagcg cgtctaccag caaggcaacg ctaggctcaa
     8941 tttccgagtc cgtaagtgtt ttctgagtat cttgcaataa cacacggaat gcaaggcttt
     9001 ttttaccttg ctccacgcct ttgccgcgat atacgtcaaa caatgcaacc tccgccacaa
     9061 acggagccgc gatgtttcgc atcgtatcca gcaagctttg tacatcaacc gcttcgtcca
     9121 ccaacacagc caaatcacgc cgcacaggca ggaacttggc aatctcgctc atgcgcggaa
     9181 cctgaacctg aaccaacgca tcgacggtga cctcgaacca gatcgctgcc tgcggcatat
     9241 cgtattgctg ttgccacttc ggatgcaatt cgccgagcca gccgatagcc tgttcgccgc
     9301 gatagatctg cgcagaacgc cccggatgca aagccggatg ggttgccgcg acgaagcgta
     9361 gaacttgagg tgcgaacagg gcttcgacgt cagccttaac gtcgtagaaa tcgactgctt
     9421 tggccgatgc gccccactgt tctgtcagac tggcgccata ggcgatagcg gacaagcgct
     9481 gcgtctgcac atattgatcg ccaaccttgg cgaaacaggc tccggcctcg aacaggcgca
     9541 cacgcgactg cttgcgattc aggttatagc gcagagcgcc aaccaggccg ccgatgaggc
     9601 tgctgcgcat caccgccaga ttgctggcga tcggattctg caacgcaacc ggcgctgcat
     9661 tgccacacag ctcgcgttcg acctgctctt ccagaaaggc gtagctgacg atctcctgat
     9721 aatcacgcgc gaccagcgtt tgctgaatct gctccattga acgctgcgat tcgctgtcag
     9781 gcaacatggt cagcgtggct tgcggtgcca gcgccgggat gttgtcgtaa ccatacagtc
     9841 gcgcgacttc ttcgatcaga tcggcctcga tggacaggtc gaaacggaag ctgggcggaa
     9901 tcacgcaaaa atcgtcaccc ttcgcagtga actcgaattg cagaccttgc agcaaagcgg
     9961 cgatctgcgc attgtccagc tcgatgccga gcacgcgtgt cacgcgcgag cgacgcaatg
    10021 caatggcagg acgagcgggc aattcacctc gcacttcgca gatgctacca gcctgaccgc
    10081 cgcagatttc cagcagcagc tgcgtggcac gctccaatgc ctgacgtgtc gcggcaaaat
    10141 ccacgccgcg ttcgaaacgg aaggaagagt cggtcgccag cccgaaacga cgggctctgc
    10201 ctgcgatcgc atcgggatgg aagaatgcgc tttccaggaa tacgtcctgc gtggtgattt
    10261 ccacgccgct gccctgcccg cccatgatgc cggccaaagc cagcacgcga gcgtcatcgg
    10321 cgatcaccaa aatgtcttca tccagcgtca cgctctgctc attgagcagc gtcaaggctt
    10381 cgtccttgcg cgcacgacgc acggcgatac ctccggacag ctgcgcaacg tcgaaggcat
    10441 gcatgggctg gcccagctcc atcatcacat agttggtgat gtctaccacc gcgttgatgc
    10501 cgcgcaggcc gctgcgctcc agacgacgca acatccagga cggcgtagct gcggcggcat
    10561 tcacgccgcg caccagacgg ccgcaataca gcgggcaggc ttctgtatcc gccaccttga
    10621 ccgacaactg ttcggacagc gcgaccggtt gttcggtgat ggccacaggc tgaagcacac
    10681 tgccggtcag cgctgcgacc tctcgtgcca gaccggcgat accggaacag tcgctgcggt
    10741 tcggggtgag cttgagcgtg aacagctggt catcgagttc cagatattca cgcagcgtct
    10801 tgccaacagg agcatcggca ggcaacagcc acagcccgtc gctcgcttcg gccaagccca
    10861 gttcgactgc ggagcacagc atgccgaaag attcgatgct gcgcaccttg gcctgcttga
    10921 tcttgaagtc accgggcagc accgcaccga tgcgggcaca tggcaccttc acgccaaccg
    10981 cgacattggc cgcaccgcac acgatggtca gcggctgcgc ctcaccgaca ttgacctggc
    11041 aaacattgag gcgatccgcg ttgggatgct tgaccacttc cagcacctcg gcgatcacca
    11101 cgttgttgaa tggaggcgcg acggcttcca gcgcctcgac ttccagacct gccatggtca
    11161 gcgcatgtcc cagttcgtcg ctggagagcg ccggattgac ccaggttctt aaccaatttt
    11221 cagaaaattt cattactttg ttctcagttg aattgcttga ggaagcgcag atcgctctcg
    11281 tagaactggc gcaggtcgtt cacgccgtag cgcagcatcg ccagacgctc cacgccaaga
    11341 ccaaaagcga aaccgagata cttctcgctg tcgatattga catgcttgag cacattggga
    11401 tgaaccatgc cgcaaccgcc gatctccagc cagccgccgt tccagctcat gtccatttcg
    11461 gcagacggct cggtgaacgg gaagaaggac gggcggaagc gcacgcgcaa atcgtcgcgc
    11521 tcgaagaaac gttgcagaaa gtcctgcacc acgcccttga gattggcaaa gctgacctct
    11581 tcatcgaccc acagaccttc gacctggtgg aacatcggcg aatgcgtcgc atcggaatcc
    11641 acacgataga cgcgccccgg cgagatgatc ttcagcggcg gctggttagc ctccatgtag
    11701 cgcacctgta ccggtgaggt atgggtacgc aacacatgct gaggatcgat gtagaaggtg
    11761 tcgtgcatcg cacgtgccgg gtgattttcc ggaatattca gcgcggtgaa attgtagaaa
    11821 tcgtgctcga tctcgggccc ggaggaagtc gcgaagccca atgaatgaaa cagctgctcg
    11881 atacgctgca aggtgcgcgt caccggatgc aggccacccg tactcaggcc acggcccggc
    11941 agggtgacat ccaacgattc agccgccagt ttctgttcca gtttgttttg ttgcagcgca
    12001 tcgcggcgcg cttgcagcgc tgcttcgata ccctgtttga cctgattgat acgggcaccg
    12061 gcagctggcc gttcttctgc ggagagttta cccaagccct tgagtagtcc tgtaaggcta
    12121 ccctctttgc ccagataacg tgctttgatc tgctccagct cagcttcgtc gctagtcgcg
    12181 gagaactgct gcagagcctg atcgagaatt tgttggagtt gttccatcga taacaccact
    12241 ttggatgcca caaacaaaaa aggaggcgaa tcgcctcctt ttttgattta gctagttctt
    12301 aaacgccgag gctggcttta gcttgcacca cgaattgtgc aaaggccgcc ttgtcgaaca
    12361 cagcgatatc agccagaacc ttacggtcga cattgatctc tgctttcttc aggccattca
    12421 tgaatacgct gtacgtcata ccttgctcac gagccgccgc attgatacgg gcgatccaca
    12481 gagtgcggaa ctgacgcttc ttctgacgac gatcgcggta agcatattgc cccgccttca
    12541 tcaccgcctg tttggcgata cgatatacgt tcttgcggcg accgcgatag cctttggcca
    12601 gggccaaaac cttcttgtgt cgagcgcgcg ctactacacc acgttttact cttggcatgg
    12661 tctactcctt atgcgtaagg catcatggcg cgaaccgatg ctgtattggt tgcgtggact
    12721 tctgtagtgc cgcgcaattg acgtttgttc ttggttgtct tcttggtcag aatgtgacgt
    12781 ttgaaggcct gagaacgctt gatgctacca ccggcgcgaa cggaaaagcg ttttttcgcg
    12841 ccgctcttgg ttttcatctt aggcatgact actccttaag ttatggtggg aggtgttttc
    12901 cgatggaaaa acttgatgac ccatacacca ctttttatcg gggattattt accgcaacct
    12961 cgctccgggc atccccttaa caccgcagcg attcaaactt atttggtctt taccttcggc
    13021 gacagcacca tcaccatctg gcggccttcc atcttgggaa actgctctac cacaccatgc
    13081 tccgccaaat cggcacgaac gcgctcgatc agacgcatac cgatttcctg gtgagccatc
    13141 tcgcgaccgc gaaaacgcaa cgtgatcttg gtcttgtcgc cttcgttcag aaacttgatc
    13201 aggttacgca acttgatgtt gtaatcaccg tcatcggtac caggacggaa tttgatttcc
    13261 ttgatctgaa cctgtttctg cttaagcttg gcctcatgct gcttcttgct ttcggcatat
    13321 ttgaacttgc cgaagtccat gatgcgacag accggaggat ccgccaacgg cgagatctct
    13381 accagatcca gttcagcctc gtccgccaag cgcaaggctt ccgcgattgc cacaatcccg
    13441 aggggctctt tttctactcc aatgaggcgc acctgaggtg cagtaatcat ctcattcaag
    13501 cgttgcgatt tatcctgtgc tattgcaatg tctccgttta ggcaaaatta aaccgcacta
    13561 cccgacttta tctccgactt gaaacgctga agcaacacct ccagcgtcat ctggcccaga
    13621 tcttcaccac tacgggtacg cacggctatc aatctcccag ccacttcctt atcgccgact
    13681 atcaactgat agggaagttt ctgcaagcta tgttcgcgta ttttataggt aatcttctca
    13741 ttgcgcaaat ccaattgcac acgcaagcca gcctcgcgca acgcttgtgc aacttcagtc
    13801 gcatattcct cttgcgcctg agagatattc atcagcacca cctggatcgg cgccaaccac
    13861 aaggggaatg cccccgcgtg atgctcgatc agaataccga tgaaacgctc catcgaaccc
    13921 aggatggcgc gatgcagcat gaccgggggt ttgcggccat tatcctcgtc gacaaactcc
    13981 gcgccgagac gtgtcggcag attgaagtcc agctggatgg tgccgcattg ccacagacga
    14041 ccgagactgt ctttcagcgt gaattccacc ttgggaccat agaaggcccc ctcgcccggc
    14101 tgaacctcgt aatccagaga attttgctgc aaggcagcgg ccagaccagc ctcggctttg
    14161 tcccaggtct cgtccgaacc gacacgcttt tccggacgcg tggataactt gaccagcacc
    14221 tcgctgaagc cgaagtcacg atagacctca ttgagcatca cgatgaaatt ggacacctca
    14281 ggctgcacct gatcttcggt gcagaagata tgcgcatcgt cctgcgtgaa gccgcgcaca
    14341 cgcatcaaac cgtgcagcga acccgagctc tcattgcgat gacaggagcc gaactcagcc
    14401 agacgcaacg gcagatcgcg gtagctatgc agcccgtgat tgaatatctg cacatgcccc
    14461 ggacagttca tcggcttcac cgcatagtcg cgattttccg atgacgtggt gaacatgttg
    14521 tcgcgatagt tatcccagtg accggatttc tcccacagac ttcgatccat gatggtcggc
    14581 gtgcgaactt cctgatagtc gtgttcgcgg aatttgcggc gcatgtactg ctcgacttcc
    14641 tgccacagcg tccagccctt gggatgccag aacaccatgc cgggagaaga atcctgcata
    14701 tggaacaact ccagctgcct gcccagcttg cggtgatcgc gcttctcggc ctcttcgagc
    14761 tggtacagat aggcgtccag ctcttccttc ttggtccagg ccgtaccgta gatgcgctgc
    14821 agcatttcgt tattcgaatc gccgcgccag taggctcccg ccagcttcat cagcttgaac
    14881 accttgagct tgccggtaga gggtacatgc gggccacgac acagatcggt gaaaccaccc
    14941 tcggtataca gcgacacatc ctgatctgcc gggatggatt cgatgatctc ggctttatag
    15001 tgctcgccga tgcccttgaa atagctcacc gcctcatcgc gcggcaacac gcggcgcgtc
    15061 acgggcatat cctgcttgac gagttcggcc atgcgcttct cgatggcggc cagatcttcc
    15121 ggcgtaaacg ggcgcttgta agaaaagtcg tagaagaagc cgttctcgat taccggcccg
    15181 atggttacct gcgcatccgg gaacaactcc ttcaccgcat gggccagcaa gtgggcggtc
    15241 gagtgacgaa tgatctcgac gccgtccgca tccttgcccg tgatgatcgc cagcgccgca
    15301 tcctgctcga tgaggtacga gcaatccacc accttgccat ccaccttgcc agccaaggcg
    15361 gctttcgcca gacccgcgcc tatgctcatt gcgacatcgg tgacagtaac ggggtgttca
    15421 aagctacgct gcgaaccatc cggcaaggtg atgactggca tgttttgtgt cctgaaaata
    15481 aaaagtgcgg cagagccgca cttggaatgt tggtaggcac gattggactc gaaccaacga
    15541 cctctaccat gtcaaggtag cgctctaacc agctgagcta cgcgcctaaa gaggcagcat
    15601 tgtatccgaa agattttcgc gcttcaaacg aaatatcacg aatatttgcg aatagcctaa
    15661 tttaataact cgccagattc tcgaaacgcg tgtattcgcc tcggaatgcc aacgcgacgg
    15721 taccgatcgg cccgttacgc tgcttgccga taatgatctc ggccttaccc ttgtcgggcg
    15781 agtcgttgtt gtagacttcg tcacgataga taaacaggat caagtcggca tcctgctcga
    15841 tagcgcccga ttcgcgcagg tcggacatca ccggccgctt gttgggacgc tgctccagac
    15901 tgcggttcaa ctgcgacagc gcaatcaccg gcacctgcaa ctctttcgcc aagcccttca
    15961 gcgaacgcga tatctccgaa atctcggtgg cgcgattctc gctggccttg cctgaatttc
    16021 cactcatcaa ctgcaaatag tccactacga tcaagcccaa ctcgccatgc tggcgatgca
    16081 ggcggcggga acgggcccgc aattccagag aggacagcgc tgccgtctcg tcgatgaata
    16141 tctttgcgtc gttaagcttg ccgagagcat gagtcaatcg cccccagtcg tcgtcctgca
    16201 aacgtcctgt gcgcaggtcg tgctgattga gcttgccgac cgaccccagc atacgcatcg
    16261 ccaactggga cgcgcccatt tccatactga aaatcgccac gggcttgccg ctgtccagcg
    16321 ccacgttctc ggcgatgttg atcgagaacg ccgtcttacc catactcggg cgccctgcca
    16381 cgatgaccag atcacccgcc tgcaaccccg aagtcatccg gtccagatcg gtataccccg
    16441 ttgcggtgcc ggtcacttcg ctgtgatcct cgcgaccata aagcgtctcg atacgctcga
    16501 ccacctgcgt caacaacggc ggcatcgaga agaaaccctg cttgcctttc gatcccgctt
    16561 cggcgatctc gaacaccttg cgctcggcct cgtccagcaa ctgcgaagca tcgcgcccgg
    16621 tcggattgta ggcactgctg gcgatctcac tgccgacttc aaccagcttg cgcatgatgg
    16681 aacgctcgcg cacgatctcg ccataacgac ggacattggc cgctgtcggc acgttctgcg
    16741 ccagcatacc cagataaggc aaaccgccgg ccttgtccag ctccccattg ttttccagcg
    16801 cctcggcaac ggtgatcaca tcgaccggtt tggcttgttc gctcagctgc acgatctggc
    16861 gaaagatcag tctatgctca tggcgataga aatcgccatc gctgaccaga tcgataatcc
    16921 tgtccagcgc ccccgattcc agcagcaagc cgcccagcac cgactgctcc gcctccaccg
    16981 aatgcggcgg aagcttgatc tgatctatct gtgcatcgga atttgaaaaa tcattcattg
    17041 ggcgaatcga acctcatggg taaacgggat gcattttaac ctgttgcagg agaacgtagg
    17101 ggcgcgatga atcgcacccc tgtaaataca aaaaaggact gtcgccagtc cttttttatg
    17161 ttgccgcgat acctgacgaa ttagtgctcg ccgagtaccg aaaccgtgat gcccactacc
    17221 acatcggtat gcaacccgat gttgacggag taatcgccga tctgcttgag gtgaccttct
    17281 ggcatatgca cttgcgactt ctccactgca tgacctttag ccaccagcgc agcagcgatg
    17341 tcggcattgg taacggaacc aaacagcttg ccgtccacgc ctgccttctg agaaatttgc
    17401 acagtcagac cggccaactt ctcgccgcga acctgagcat cagccagctt ggctttgtca
    17461 gccgcttcga tctcggcgcg acgcgcttcg aactcggcca tgttggcagc cgttgcacgc
    17521 ttggctttgc cttgcgggat caagaaatta cgagcgtagc catttttaac cttgaccaca
    17581 tcacccagtt ggccaacgtt gaccactttt tccatcaaaa taatttgcat gttctgctcc
    17641 ttcttaatgc aggtcggtgt acggcagcaa agccaggaaa cgagcgcgct tcacggcggt
    17701 gctcaattga cgctggtagc gtgtcttggt gccggtgatg cgagctggga tcagcttgcc
    17761 gttttcggag atgaattcct tgaggatgtt cacatccttg taatccactt ccgcgatctt
    17821 atctactgtg aagcggcaga acttgcgacg cttgaacaga ttgttacggg aagaacgctt
    17881 gttcttgtca tctttcttct tgtcggatgg acgagccatg atgtttcctt attccaaaat
    17941 aatatcgttg atatgcaaaa ccagttgtcg actgcgcata ccgcgctgag ccaagaaacc
    18001 acgcacctta acggtttgtc cgattgcgaa ctggccgacc ttttgagcca cttcggcaaa
    18061 tgccagtgca tcaacttcac actgtacctg cctctcaccg ttcgcttccg gctgctgcga
    18121 gctgtgacgc aacgtcaact ccactctcgc cacgcctgcc ggtgtatacc gcaaaccttc
    18181 caatgcgatc agctcaccgc tgatgacaac ctgattacgc gccaattgcc aatgacttaa
    18241 gccgctgcgc cttcttcagc ccttgctgct ggagcagaag cttcggtcgg tacggaacgg
    18301 gatttctctt ccttcatcat tgcagaagct tccgtaacgg cagccttggt cttgacggtc
    18361 agatgacgca gaaccgcatc attgaagcgg aatgcatgct ccagctcgtc cagggtttcc
    18421 tggttgcatt cgatattcat cagcacgtag tgtgccttgt gaattttttg gatagggtaa
    18481 gccaactgac ggcgacccca gtcttccagg cgatggattt gaccatcctt ggaagtgacc
    18541 agtgtgcgat aacgctcgat catcgcgggc acttgctcgc tctgatcggg gtgcacgata
    18601 aacacgattt cgtaatgacg cattacatct ccttacggtt aacagccccc cgccatgcgt
    18661 agcggtggag caaggtcgag agccgaccat cggctctacg gggcgcgcat tataacgaaa
    18721 aagcctgtgc agtcaaggaa tttggcgctt tcagcccggc agcgcacgct gtcttgccgc
    18781 ctcgaacagg cagactgcgg cggcagcggc cgcgttcagc gactccacct tgcccggcat
    18841 cgggatgatg aagggccgat gaactgcatc cagcaaggct tgcgacagcc ctgccccttc
    18901 gttgccgatg accagcgcca gatcgccgcg cagatcgcaa tcgtacaggc tggccgtggc
    18961 ctgcagcgac gccgcatgga tcgtgcccgg gaaggtcgcc gcgaccgcca gcaagtcttc
    19021 cgcctcgtag atgggcagcg agaaatgccc gcccatcgcc ccgcgcagca cgcgcggcga
    19081 ccagacatcg gcgcagccct tggacagata gaccgcatcg cagcccgccg cagcggccgt
    19141 ccgcagcatc gaaccgaggt tgcccggatc ctgaatgtct tccagcaaca acgcgaaacg
    19201 gtgatcggcc acggggggct gcggaatctt gaccagcgcg aggatgccgg tcggcgtctt
    19261 caactcggtc agttcggcaa acagcttgtc gtccagctgc gacatgggca cgcctgcggc
    19321 gcgcttgagc agcgcctcga cctcatcgtc tttcagtgcc gcactgttca gcaggatatg
    19381 caacggcttc tcgccggcat ccaggtacga ggtcaacaga tgcgtgccgt ccagcagggt
    19441 ctggcgagcc ttgccgcgct gccgcgccga gccggccagc ttgacgagat ctttgaagaa
    19501 cggattgtcg cgggatagga tcagtttcat tttttcatgg gcgggtgaga tggctattcg
    19561 gcgtcatcgg gcccgaaagg ccagccttcg ggagcgtccg gatcaatctt cggctggctg
    19621 ggcgcgggtg ccgataccgg cgaaggtgca acctcggatg cagctgcggg cggagcgact
    19681 ggagcgactg gagcggctgc gagagcattc tcggcggcgg tttcctgatc cagcaaggtg
    19741 ccgatttcat ccagcgaggg cagttcctgc aagccgcgca gattgaggtc gtcgagcaat
    19801 tgtttggtgg tggcaaacaa ctccggcttt ccgggcgcat cgcgggtgcc gacaacttcg
    19861 acccagccgc gcgcctccag tgtcttgagg atttgcgagg aaacggcgac gccgcgaatc
    19921 tcttcgatat cgccgcgcgt gaccggctgg tggtaggcga tgatggccag cgtctccagc
    19981 acggcacgcg aatagcgcgg cggcttctgc ggattgagcc ggtcgagata tggctgcatc
    20041 tcgggcttgg cgcggaagcg ccagccgctg gcgacacggc tcagctccac gccgcgcccc
    20101 tgccagtcct gcgccagcga ttcgagcagc atctccatct gacgcgtgtc cagcgagacg
    20161 tcgctgagct tcttcagctc ggcgaccgac agcggttcgg tactggtcag cagcgcggtt
    20221 tcgagcacga tcttgaattg cacggtgttc attttcccgc caaacagatc gacgcgaatc
    20281 gcgtcatcgc tccgctcggc tacgcccgtc acgggcgagg ctgcttcact ctgcactgat
    20341 ggctcggctg gttcggacat agatgctccc aaatgtttcg ctttgctgga tttctaccag
    20401 atattctttt gccagttcga gcacggcgat gaaggtcacc accagtttcg gcacgccccc
    20461 ttcgatgtcg aacagatgct cgaaaggcac gtactccccg ctcttcaggc aacgcagcac
    20521 atgcgtcatc tgctcgcgca ccgacaactg ttcgcgccgc accttgtgat gcttgttgag
    20581 tttggcccgc gccagcaacc ccatccaggt ctggcgcagg tcttccacgc tgacctcggg
    20641 cagacgctcc tgcacggtgc gttcgatcag cacctgcacg acctcgaagt cccgcccggc
    20701 ctgcggcaat gcgttgagca gctgcgcggc ctgcttcatc tgctcgtatt ccagcagacg
    20761 gcgcatcagc tcggcgcgcg gatcttccgg ctctgcatcg ctgatcttgg gcggacgcgg
    20821 caacagcatg cgcgacttga tctcgatcag cacggcggcc attagcagat attcggcggc
    20881 cagctccagc cggttctgct gcatcagctc gatatagctc atgtactggc gagtcagctc
    20941 cgccatcgga atatccagca catcgaggtt gtgcttgcgg atcagataca gcaacaggtc
    21001 gagcggcccc tgaaagtttt ccagcaccag ctccagcgca tccggcggga tgtacaggtc
    21061 ctgcggcagc tccagcagcg cctctccgtg gatgcgcgcg accggatgca atggcagctg
    21121 ggttccgctc atacttagct gtagctcagc cccatcgcct cgcgcacgtc gcgcatggtc
    21181 tcggaggcca gcttgcgcgc cttctcgcag ccgtcggcga tgatgttctt caccagcgcc
    21241 ggatcgtcca gatagatctg cgcgcgctcg cgcatcgggc gctgctcgtc gagcaccgcg
    21301 tcgatcaccg gctgcttgca ttcgatgcag ccgatgcccg cgctcttgca gccgtgctgc
    21361 acccattctc tggtgtcctc gccggaatac acctgatgca gctgccatac cgggcagcgc
    21421 gccggatcgc ccggatcggt gcggcggatg cgcgccggat cggtgggcat ggtgcggatc
    21481 ttcttgccca cactggcctc gtcctcgcgc agggtgatgg tgttgttgta ggacttggac
    21541 atcttctgcc cgtccagccc cggcattctg gaagcgaccg tcagcatcga ctgcggctcg
    21601 gacaggatca tcttgccgcc gccttccaga tagccgaaca gccgctcgcg gtcgccgtgg
    21661 gagaggctct gctgctcgtc cagcagggac ttggcggaca gcagcgcctc gtcgtcgccc
    21721 tgctcctgaa aacgcacacg cagctcgttg tacagcttgg ccttcttgct gccgagtttc
    21781 ttgacggcgg cctcggcctt ctcctcgaag cccggctcgc gtccgtagat gtggttgaag
    21841 cgccgcgcga gctcgcgggt gaattcgata tgcggcacct gatcctcgcc caccggcacc
    21901 tgggtggcgc ggtagatcag cacgtcggcg ctcatcaaca gcgggtaacc cagaaagcca
    21961 taggtggaca gatccttgct ggtcagtttc tcctgctggt ccttgtaggt cggcatgcgc
    22021 tccagccagc ccagcggcgt gatcatcgac agcagcagat gcagctcggc atgctccggc
    22081 acgcgcgact ggatgaacag cgtggcatgg gcgggatcga caccggcggc gagccagtcg
    22141 accaccatgt cccacacgct ctgctcgatc acctgcggcg tgtcgtagtg cgtggtcagc
    22201 gcatgccagt cggccacgaa gaacaggcac tcgaactcgt gctgcatctg cacccagttc
    22261 ttcaacaccc cgtggtaatg ccccagatgc aaacttccgg tcgggcgcat gcccgataac
    22321 actcgatcag caaacatagt cagattccaa acaacataga gataagttta tataccagcc
    22381 ccaccagcgg cgacagtatc ttgcccagca caggggtgac ggccatgaaa atcaggatga
    22441 acatgccgta gggctcgatc cgcgacagct gcacggcctg ccggtacggc agcaggctga
    22501 ccgcgacgcg cccgccgtcc agcggcggca gcggcaacag gttcagcacc atcagcacca
    22561 cattgatctt gatgccgatc tcggccatgc ccagcagcgg ttcggcaaaa taattcccgg
    22621 ggaacagcca ggtcagcttg aagatcagcg cccaacccag cgccatcacc agattcgagg
    22681 cggggcctgc cagcgccacc cacagcatgt cctgcttggg acggcgcagc gcggagaaat
    22741 tgaccggaac cggcttggcc cagccgaaca tgatgccgcc gaacaacaac gtcaacagcg
    22801 gcagcaggat ggtgccgacc ggatcgatgt ggcgcaccgg gttcaggctg atgcgcccct
    22861 gctggtacgc ggttgtgtcg ccgaagtggc gcgccacata accgtgggcc gcctcatgca
    22921 acgtgatggc gaacagcacc gggatcgcgg ccagactgac gagctgaacg aagtgggcga
    22981 tgtccattat tctgcgatac cgaagggtga gatgtcgcct ttgccgcgac gcagcagcac
    23041 cggcgtatcg ccggtcagat cgatcacggt ggtgaaatcg acgcccacca taccgccgtc
    23101 gatcaccaga tcaacctgct tctccagcag atcgcggatg cgctcgacat cgttcagcgg
    23161 ccactcctcc tccggcagta tcagcgtgct gctcaggatc ggctcgccca actcttcgag
    23221 cagagccagc gccaccggat gatcgggaac ccgaatgccg atggtgctgc gcttgggatg
    23281 ctgcacacgg cgcggcactt ccttggtcgc atccagaatg aaggtatagc tgcccggcgt
    23341 gttggccttg agcaggcgga acttggcgtt gtccacccgc gcatagttcg atatctcgga
    23401 caggtcgcgg cacatcagcg tcaggtgatg ctgctcgtcc agctggcgta tctggcggat
    23461 gcgcgtcacc gcagtcttgt cgtcgagatg acagcccagc gcatagcagg agtcggtcgg
    23521 atacaccacg atcgcgccgt cgcgcagcat ctgtgccgcc tgacggatca ggcgcggttg
    23581 cggattgccc ggatgaataa cgaagaattg cgacatcaaa ccaccttctg catgaatgtt
    23641 tccccccagc tttcccacac cgggcggcag cccagcggga aatccggcaa ccgccccata
    23701 tcgcggtaac tctcgttcgg gctatgaaaa tcggaaccgc aggaacacat cagctcgaac
    23761 tcctcggcat aacgggcgaa ttcggcgtat tgctccggcg tatggctgcc ggtcaccacc
    23821 tcgagcgcct gcccgccgca ttcgacgaac tcgaccagca gctcgcgcat ggtcttcttg
    23881 cccatgctct ggcgtccgac catgtagcga ccgggatggg ccagcaccgc caccccgccg
    23941 ctgccgcgta tccagtctat cgcgtcctgc aggctggccc attgatgcgg cacataaccg
    24001 ggcttcccct tagccagata gcggttgaac acgctcttca cgtccttgca gtgcccggcc
    24061 tcaaccagat agcgcgcgaa gtgggtgcgc ccgatcatgt tcgggttgcc cgcatactgg
    24121 tatgcgcctt gcagggttcc gccgatgccg gtcttcgcca actcatcggc cattttctgg
    24181 gcacgctcgc cgcgtccgct gcgcacggtc tgcaggccct cggccagcgg cgcatagtgc
    24241 ggatcgatgt tcagcccgac gatatgcagg gtgtgcgtgc gccaggacac ggatatctcc
    24301 acaccgttga cgaaacccat gccgcactga gccgctgcgg cacgcgcctc gtccagcccg
    24361 tccacattat cgtgatcggt cagcgccagc accttcacac cgcgctccgc cgcccgcgct
    24421 accagctcgg tcggggtcag cgtgccgtcg gaaatgatgg aatggcaatg caggtcgtaa
    24481 tcaagcaccc gaacacctcg ggccatcgtt gcgagtcagg gacaacaaag agatactcat
    24541 gcgtcgccgc cacccttctt catgaccttg ccctcttcgg cgcgcttgcg gcgcacctcc
    24601 ttggggtcgg cgatcagcgg gcgatagatt tcgat
//
LOCUS       DASUIV010000128         5175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1146899, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000128 DASUIV010000000
VERSION     DASUIV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146899"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFW59_04875"
     CDS             <1..942
                     /locus_tag="VFW59_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5363578.1"
                     /translation="GSSGGSAAAIAAGIVHAALGTDTAGSIRVPAACCGIVGFKPSYD
                     VLPRDGVMDLAPSLDHVGPLGRTVADAAALFAAMLDLPSMPAWRYTGLAGRRIARLTG
                     YFDQPLDPCLRDALAEAMRTLAEDGAACSVTQIDGMEAAAAIQLNTICAEASATHNER
                     LQQRGEALGEDVRVRLEIGNFLPGHWYVKAQKMRRQLADHTDAAFGLSDFLICPTMRA
                     PAPPVGAARVEIEGRSYALHTAVTQLTMPFNLTGHPAISIPWSQTQDGVPLALQIIGR
                     HGTDWQVLAVAERLEHLSPWSKRQAATNSKKKCHAGN"
     gene            929..1819
                     /locus_tag="VFW59_04880"
     CDS             929..1819
                     /locus_tag="VFW59_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5363579.1"
                     /translation="MLAIDLLVNGLIFGLFYALMAVGLAMIFGVLKVVNFAHGEFYMV
                     GSYAYVLISLKLGVSPWIALPVAAAAGAALGWLVERTLMRPLYSGYASWSIMKDEYAV
                     VVTFGLSLLLINLVDKIVGPYPIRGPELIETSRFSIGPIMLNGQKLIAAGISLAILTG
                     LALFIKKSLWGRQIQAVAQNRLGASLAGIDATRTTSMIFAIAGLLAALSGALLAPVIN
                     PSPDVGAFPAIKSYVIVVLGGMGSIWGAMIAALFLGVFEAFFAVYVSYAYRDAFGLLL
                     LILVLIFRPQGLFGEKGREV"
     gene            1816..2790
                     /locus_tag="VFW59_04885"
     CDS             1816..2790
                     /locus_tag="VFW59_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5363580.1"
                     /translation="MSTPRNLWANRLRTDFRAALGLLAVLACVPFFVTSPYSLGIVIV
                     SMYFAMLAMGWNLLAGFTGQFSLAPAAFAMLGAYTTGLLNYHWQAPLALGLPAAVLGT
                     ALVGWALGRLILRLRGPYLSLTTLSFAEIARVVISNSHEFTRGDQGLNVPTLMQSRVG
                     YYYLFLAVLALTLVGLYLLMRSKVGRFWLAIRDDSIGAETRGIDVVRYKTLAFGLSCA
                     ICGFAGALYGTFSQLVSPELGLLLQTGLVISMVVIGGIGTLTGPILGALLVYLSSEWL
                     REIGGIQLIVFSALVIIFARFFRTGLWGLVTSWRPRLKIAPDAVKEPT"
     gene            2787..3509
                     /locus_tag="VFW59_04890"
     CDS             2787..3509
                     /locus_tag="VFW59_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5363581.1"
                     /translation="MSLLMTRGLSIHFGGVAAVDDVSFDVEEGEILGLIGPNGSGKTT
                     VLNMLSGFLQPDAGEVIFAGENVTRLTTVELARRGLLRMFQMTRVFGRITTLDNMLVA
                     GMAMGLTEKETNQRAQVLLEELAMTHVQYLDAGQLSGGQRKLLEFGMCFIKPPRMALL
                     DEPFAAVHPVMREAMANFIRRRHAMGQTFVLVSHDMPIVVELCQRSVCMNAGKVIAVG
                     STRSVLESTPVIEAYLGSSSHV"
     gene            3502..4236
                     /locus_tag="VFW59_04895"
     CDS             3502..4236
                     /locus_tag="VFW59_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5363582.1"
                     /translation="MSELLLNVENVTAGYAQDIDVLRDVSLQVHAGKVSGLIGLNGAG
                     KSTVVKTICGFLSPKAGQVSFMGKPISGVAPHQLIDLGICCIPQESSLFPYLTVEENL
                     LLPLQGRRKKFGGVIAQRFEEVLAAFPAVREKRRAQAGDLSGGQQKQVEFAKAWLQQP
                     RMCLIDEPSIGLSPIMAEEVFSWIEKFTQSGMGILLIDHNVRRVVRMSDHVHVLSLGR
                     ITAAGKKEAFQGDLHEQVKQWLGLNF"
     gene            4236..4742
                     /locus_tag="VFW59_04900"
     CDS             4236..4742
                     /locus_tag="VFW59_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEX5363583.1"
                     /translation="MRRIACQSVTAENFFRFGLVIDAKGATPEFINEGTTERFPDLAS
                     FDPGVQAASPVIGIYLANARTFPLRITKLERHRQASQVFIPLGMHRFVVVVAPGRETP
                     DWEQVTAFLTSPGQGISLHRGTWHHGLVALNDADRFAVIEGGNYRVDTEEHPAQEERW
                     LDDPVTSS"
     gene            4921..>5175
                     /locus_tag="VFW59_04905"
     CDS             4921..>5175
                     /locus_tag="VFW59_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363584.1"
                     /translation="MSDNSVNNPVNDAVTLTQTLLRMNTINPPGNEDQCTRYLAELLS
                     AAGYDCRLVDFAPHRSTLVARIGGRSDKPPICFTGHVDVVP"
BASE COUNT          987 a         1484 c         1569 g         1135 t
ORIGIN      
        1 ggatcgagcg gcgggtccgc cgccgccatt gccgccggca tcgtccatgc cgcactcgga
       61 accgacacgg cgggctcgat ccgggtgccg gcagcctgtt gcggcatcgt cggtttcaag
      121 ccgagctacg atgttttgcc cagggatggc gtaatggatc tcgcgccctc gctggatcat
      181 gtaggtccat tgggacgaac cgtggccgat gccgcggccc tgtttgccgc catgctggat
      241 ttgccgtcga tgccggcctg gcgctatacc ggcttggcgg ggcgaaggat tgcccggctg
      301 actggctact tcgatcagcc gctcgacccg tgcttacgag acgccctggc cgaagccatg
      361 cggacgctcg cggaggatgg cgcagcctgc tcggttaccc agatcgatgg catggaagca
      421 gcggctgcca ttcagttaaa cacgatttgc gccgaagcca gcgctaccca taacgagcgc
      481 ctgcagcagc gcggagaagc gctcggcgaa gatgtacggg tgcggctcga gatcgggaat
      541 ttcctgcccg gacactggta tgtcaaagcc cagaagatgc ggcgccagct ggccgatcac
      601 acggacgccg cgttcggcct gtccgacttc ctgatttgcc caacgatgcg cgcgccggcg
      661 ccacccgtcg gcgcagcccg tgtcgagatc gaaggacgct cttatgcgct gcataccgca
      721 gtgacgcaac tgacaatgcc attcaacttg accggacacc cggccatttc cattccatgg
      781 agccagacgc aggacggcgt gccgctcgcc ctgcagatca tcggcagaca tggcacggac
      841 tggcaggtgc tggccgtggc agaacggctt gaacacctgt ctccatggag caaacggcaa
      901 gcggccacta acagcaaaaa gaaatgccat gctggcaatt gacctgctcg ttaacggctt
      961 gatcttcggc ttgttctacg cgcttatggc ggtcggactg gccatgattt ttggcgtatt
     1021 gaaagtggtg aactttgccc atggcgagtt ctacatggtg ggttcttatg cgtatgtgct
     1081 catttcgctg aaactgggcg tgtcgccgtg gatcgcgttg ccagtcgcgg cagccgcagg
     1141 cgcagcgctc ggatggctgg tcgaacgcac cttgatgcgg cctctgtaca gcggctacgc
     1201 aagctggagc atcatgaagg atgaatacgc agtcgtcgtc acgtttggtc tctcgctgct
     1261 gctcatcaac ctggtagaca agatcgtcgg cccctatccg atccgcggtc cggagctcat
     1321 cgaaaccagc cgtttctcca tcggaccaat catgctcaac ggtcaaaagc tgattgcggc
     1381 aggcatctcg ctggcgatac tgaccggcct tgccttgttc atcaagaagt cactgtgggg
     1441 ccggcaaatc caggcggttg cacaaaaccg gctgggtgct tcgctagcgg ggattgacgc
     1501 cacgcgcacc accagcatga tctttgccat cgccggtttg ctcgcagcat tgtcaggcgc
     1561 actgctcgcg ccggtcatca atccttcccc ggacgtcggt gctttcccgg ccatcaaatc
     1621 ctatgtcatc gtcgtgcttg gcgggatggg ctcgatttgg ggtgcgatga tcgccgccct
     1681 gttcctgggg gtgttcgagg ccttcttcgc agtctatgtg tcctatgcct atcgcgacgc
     1741 attcggcctg ctgctgctga tcctggtcct gatattccga ccgcaaggtt tgttcggaga
     1801 gaaaggacgt gaggtatgag caccccgcgc aatctctggg ctaaccggct acgcacggat
     1861 tttcgggcgg cgctaggctt gctggcagtg ctggcctgtg ttcctttctt cgtgacctcg
     1921 ccctattcgc tcggcatcgt catcgtcagc atgtatttcg cgatgctggc gatgggatgg
     1981 aacctgctgg ccggcttcac cggtcagttc tcgcttgcgc cagcagcatt cgccatgctc
     2041 ggggcctaca ccacggggct gcttaattat cattggcagg cgccgcttgc gctgggcctt
     2101 ccggccgcag tgctcggcac ggcgctggtg ggatgggcac tgggacggct tatccttcgc
     2161 ttgcgtggcc cctatttatc cctgacgacg ttgtcgttcg ccgagatcgc acgggtcgtc
     2221 atcagcaatt cgcatgaatt tacgcgtggt gaccagggcc tgaacgtgcc aaccttaatg
     2281 caaagcaggg tcggctacta ctatcttttt ctcgcggttc tggcattgac gttggtcgga
     2341 ctttatctgc tcatgcgctc aaaggtaggg cgtttctggc ttgcgattcg ggacgactcc
     2401 atcggtgccg agacccgcgg cattgatgtg gtgcgctata aaaccctggc ctttggactg
     2461 tcgtgcgcaa tctgcggctt tgccggtgcg ctctacggca cattcagcca gctcgtcagt
     2521 cctgaactcg gcctgctgct gcaaaccggg ctggtgattt cgatggtagt cataggcggt
     2581 atcggcaccc tgacgggtcc gatcctcggt gcgctactgg tctatctttc gtcggaatgg
     2641 ctgcgggaaa ttggtggcat acagctgatt gttttttctg cgctggtgat tatcttcgcg
     2701 cgttttttcc gcaccggact ctggggcctg gttactagct ggcgcccgcg cctgaagatc
     2761 gcgcccgatg cagtgaagga gccgacatga gcctgctcat gacaaggggc ctttccatac
     2821 atttcggtgg cgtcgcggcg gtggacgatg tcagctttga cgtcgaggaa ggcgaaatcc
     2881 tcggcttgat cggacccaat ggatcgggta agacaacggt actcaacatg ctgtccggat
     2941 ttctccagcc cgatgccggc gaagtcattt ttgcaggaga gaatgtgacc cgcctaacga
     3001 cggttgagct ggcccggcgc ggattgctgc gcatgttcca gatgacccgg gttttcggac
     3061 gcattacgac actcgacaat atgctggtgg cgggaatggc gatggggttg accgaaaaag
     3121 agaccaacca gcgcgcccag gtgttgctcg aagaactggc catgacgcat gtccagtatc
     3181 tcgatgcggg tcagttatcg ggaggccagc gcaagcttct tgagtttggc atgtgcttca
     3241 tcaagccgcc aagaatggcg ctgcttgatg aaccctttgc ggccgtccat ccagtgatgc
     3301 gggaagccat ggccaacttc atccggcgcc gccatgcgat gggccagacc tttgttctgg
     3361 tcagccacga catgcctatc gtcgtcgagt tatgccagcg ctcggtgtgc atgaatgccg
     3421 gcaaggtcat tgcggtggga tcgacccgca gcgtgctgga aagcacgccg gtgatcgagg
     3481 cttatcttgg gagttccagc catgtctgaa ttgctgttaa acgtcgagaa tgtgacggca
     3541 ggctatgcgc aggacatcga tgtgttgcgc gatgtttcgc ttcaggtcca tgctgggaaa
     3601 gtcagcggat tgataggctt gaacggcgca ggaaaatcca cggtcgtcaa gacaatctgc
     3661 ggctttttaa gcccgaaagc cggacaagtc agctttatgg gaaaacctat ttcgggagtc
     3721 gccccgcacc agctgatcga tctcggcatc tgctgcatcc cgcaggagtc cagcctgttt
     3781 ccatatctga cggttgagga gaacctcctg ctgccattac aggggcgcag gaaaaaattt
     3841 ggcggcgtca ttgcgcagcg ctttgaggag gtgctggcgg ccttccccgc ggttcgcgag
     3901 aagcgccggg cgcaagccgg cgacttgtct ggcggccagc agaaacaggt agagttcgcg
     3961 aaagcctggc tgcagcagcc acgaatgtgc ctgatcgacg aaccgagcat cgggctgtcg
     4021 ccgatcatgg ccgaagaagt attctcctgg atcgaaaaat ttacccagtc cggcatggga
     4081 attttattga tcgaccataa cgtcaggcgc gtcgtgcgca tgtctgatca cgtccatgtg
     4141 ctgagccttg gccgtattac agcggcgggc aagaaggaag ccttccaagg cgatctgcat
     4201 gaacaggtga agcagtggtt ggggctgaac ttttgatgcg gcgtatcgca tgccaaagtg
     4261 tcacggcgga aaactttttc cgcttcggac tggtgatcga cgccaagggc gctacgccag
     4321 aattcatcaa cgagggcacc acagaacgat tcccggatct ggcctcgttc gatccgggtg
     4381 tccaggccgc cagcccggtg ataggaattt accttgcaaa tgcacgtacc tttccactga
     4441 gaattacaaa gctcgaacgc catcggcagg ccagtcaggt ctttattccg ctgggcatgc
     4501 atcgctttgt cgtggttgtg gcgccgggcc gggaaactcc cgattgggaa caggtcacgg
     4561 cattcttgac gtcgcctggc cagggcatca gtttgcatcg gggaacctgg caccatggcc
     4621 ttgtcgccct gaatgatgcg gaccggttcg ccgttatcga gggcggaaac taccgggtgg
     4681 acacagaaga acacccggcg caggaagaac gatggcttga tgaccccgtc acatcatcgt
     4741 gatcacccat tcagccactg tactgctgtt ggagcagaac gtgcgcctga cgctggcgat
     4801 tgcccttgat ggtgacgtac ggtcctaaga gcgccggtta gcgcaattta gaacccagaa
     4861 taaagtacat ggctggtcga ttcgtgccgc tatttcggct aacaaaatat ggagacctca
     4921 atgtctgaca attcagtaaa caaccctgtc aacgatgcgg taacgctgac acagaccctg
     4981 ctgcggatga acaccatcaa tccgcccggc aatgaagatc aatgcacccg gtatctggca
     5041 gagctgctgt cggccgcggg ctacgattgc cgactggttg acttcgcacc gcaccgcagc
     5101 accctggtcg ctcgcatagg cggcagatcc gacaagccgc cgatctgctt caccggccat
     5161 gtcgacgtgg tgccg
//
LOCUS       DASUIV010000129         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_959815, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000129 DASUIV010000000
VERSION     DASUIV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959815"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VFW59_04910"
     CDS             complement(<1..1556)
                     /locus_tag="VFW59_04910"
                     /inference="COORDINATES: protein motif:HMM:NF006144.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5363585.1"
                     /translation="MQYHLNGFKPGNYQIPDAAREPAAPPPMVDLPKEVDVLIVGCGP
                     AGLALARQLADFADIKTCIVEQKSGPMLFGQADGISCRTMEIMEAFNSSELLAKEAYQ
                     LHQNAFWEPDAQNPQHIKRTHKVPDARLGLSEFTHCVVNQARLHDILLDGMAHSVTHL
                     VPHYNRRLLELVVDQSLTQDLNAHPITATFERNDASQSSKVETIKARYVVGCDGARSA
                     VRKNLGIELQGDSANKAWGVMDVLLATDFPDIRVKSFIQSKDNGAVMLVPREGGYLVR
                     LYVELDLLGKDQRASQLNLTVDDVIAKAKLIMNPYQVDVKEVAWWSIYEVGQRVAERF
                     DDTPVGNPDGLIPRAFVAGDACHTHSPKGGWGLNTSLPDTFNLGWKLAAVLQGKAQPK
                     LLQTYATERRKVAFMLINADREMSALVATRANKDATAAKTSTADIEKFIARQNGFIAG
                     TSIGYDESWLCTGHEHQALASGFPIGQRFHSAQAVRVADGCPVHLGHLNKADGRWRIF
                     IFADAQSPLDP"
     gene            complement(1863..2029)
                     /locus_tag="VFW59_04915"
                     /pseudo
     CDS             complement(1863..2029)
                     /locus_tag="VFW59_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
BASE COUNT          354 a          652 c          647 g          376 t
ORIGIN      
        1 ggatccagcg ggctctgcgc atcggcaaag atgaagatgc gccagcggcc atccgccttg
       61 ttcaggtgcc ccagatgcac cgggcaacca tccgcaacgc gcacggcctg ggcggaatgg
      121 aaacgctgcc cgatcgggaa gccgctcgcc agcgcctgat gctcatgccc cgtgcacagc
      181 caggactcgt catagccgat tgaggtgccg gcgatgaagc cgttctgccg ggcgatgaac
      241 ttctcgatgt cggcagtgct ggtcttcgct gcggtagcgt ccttattggc gcgggtggcg
      301 accagcgccg acatttcgcg gtcggcgttg atcagcatga aggccacctt gcggcgctcg
      361 gttgcgtagg tctgcaacag tttcggctgc gccttgccct gcagcaccgc cgccagcttc
      421 cagcccaggt tgaaggtatc gggcagcgaa gtgttcaggc cccagccgcc tttcgggctg
      481 tgggtatggc aggcatcgcc ggcgacgaag gcacgcggaa tcagaccatc cggattgccc
      541 accggggtgt cgtcgaagcg ctcggcaacg cgctgcccga cttcgtagat ggaccaccag
      601 gcgacttcct tcacatccac ctgatacgga ttcatgatca gcttggcctt ggcgatgacg
      661 tcatcgacgg tcaggttcag ctgcgaagcc cgctgatcct tgcccagcag gtcgagctcg
      721 acatacaggc gcaccagata gccgccctca cgcggcacca gcatcaccgc gccgttgtcc
      781 ttcgactgga tgaagctctt cacccggata tccgggaagt ccgtcgccag cagcacgtcc
      841 atcacgcccc aggccttgtt ggccgagtcg ccctgcagct cgatccccag gttcttgcgc
      901 accgcactgc gggcgccatc gcaaccgact acatagcggg ccttgatggt ttcgaccttg
      961 ctcgactggc tggcatcgtt gcgctcgaag gtcgcggtga tcggatgcgc gttcagatcc
     1021 tgagtcaggc tctgatccac caccagctcc agcaggcggc ggttgtaatg cggcaccagg
     1081 tgggtgaccg aatgcgccat gccgtcgagc aggatgtcgt gcaggcgcgc ctggttcacc
     1141 acgcagtggg tgaactcgga cagccccagc cgcgcatccg gcaccttgtg ggtgcgcttg
     1201 atgtgctgcg gattctgcgc atccggctcc cagaaggcgt tctggtgcag ctggtaggct
     1261 tccttcgcca gcagctcgct gctgttgaag gcctccatga tttccatggt gcggcaggaa
     1321 ataccatccg cctggccaaa cagcatgggc ccggacttct gctcgacgat gcaggtcttg
     1381 atgtcggcaa aatccgccag ctgccgcgcc agcgccaggc ccgccgggcc acaaccgacg
     1441 atcagcacat cgacctcttt cggcagatcg accatcggcg gcggcgctgc aggctcacgc
     1501 gccgcatcgg gaatctggta gttgccgggc ttgaagccgt tcagatggta ttgcatgagc
     1561 agaatcctct tgtcgttgtt gtcggctgag cgcacgtcag gacgccgtta cgctgttttg
     1621 acactgagca acagcttagg tcgataagag acactgctgt gtcccctgaa aatggggaca
     1681 cggggtgtga agtggtttgc cggtatcgaa tcactggccc agtccggaac ggtgcaccgg
     1741 gattggtaga aaaccgctgc gcgagtcttc gaccctgcac ctgaacccca gacgctccgc
     1801 gtccagccgc aggagcggcc gggttgcgcg cccacgcagg agcgtgggcg cgatcatcat
     1861 gcgagcgagg cgagggtgcc gaactgctcg gccaccacca gcggcgcatg gttgggcagc
     1921 atgatcccgc ccgaccccac gcggatggac ttcgtgccgg cggcgacatg cgcgatcaca
     1981 accgccgtgg cggcggaggc gatgcccggc atgttgtggt gttcggcca
//
LOCUS       DASUIV010000130         5897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_496849, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000130 DASUIV010000000
VERSION     DASUIV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496849"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFW59_04920"
     CDS             <1..940
                     /locus_tag="VFW59_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX5363586.1"
                     /translation="YALARERLRELIGMMRLPVEIPYAPATPRVEAKSEFGEAAFKQA
                     VEKAKQYIFDGDIMQVVLAQRMSQPFPASPLSLYRALRSINPSPYLFYYDMGDHHVVG
                     SSPEILARLEGDTVTVRPIAGTRPRGKTPQQDAALADELLADPKELAEHLMLIDLGRN
                     DIGRVAENGTVRLTDKMVIERYSHVMHIVSNVEAKLKQGLDAMDVLKATFPAGTVSGA
                     AKVRAMEIIDELEPSKRGIYAGAVGYLGFNGDMDVAIALRTAVVKDKMLYVQAGAGIV
                     ADSVPENEWIETQNKARAVLRAAEMVLDGLDAQAHR"
     gene            944..2572
                     /locus_tag="VFW59_04925"
     CDS             944..2572
                     /locus_tag="VFW59_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5363587.1"
                     /translation="MLRLTEIKLPITHPEGDIRTAILKRLDIADAELIRYIVFKRGVD
                     ARKKNGILFAYTLDVEVRDEAEVLARFKNDPHVGITPDTSYHFVTQAPQSLAERPVVI
                     GMGPAGLFAGLVLAQMGFRPLILERGKAVRERTKDTWGLWRQGKLDPESNVQFGEGGA
                     GTFSDGKLYSQVKDPRFLSRKVLDEFVKAGAPDEILYESHPHIGTFRLVGMVEKMRET
                     IASLGGEIRFQSRVDDIEIENGEIRGVVLSNGERIATNHLVLAIGHSARDTFEMLHKR
                     GVYIEAKPFSIGFRIEHPQSLIDRARYGSNAGNPLLGAADYKLVHHARNGRSVYSFCL
                     CPGGTVVAATSEPGRVVTNGMSQYSRNERNANSGIVVGISPEDFPVDPENPDNPLAGV
                     ELQRRWESRAFELGGGTYQAPGQLVGDFLAGKPSTQFGEVQPSYTPGVHLTDLSTALP
                     DYAIAAIREALPAFAKQIRGFDLSDAVLTGVETRTSSPVRIKRRDDDLQSINTRGLYP
                     SGEGAGYAGGILSAAIDGIKIAEAVALNMMHAKN"
     gene            2586..3155
                     /gene="pabA"
                     /locus_tag="VFW59_04930"
     CDS             2586..3155
                     /gene="pabA"
                     /locus_tag="VFW59_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5363588.1"
                     /translation="MLLMIDNYDSFTYNLVQYFAELGADVVVHRNDEITVEQIEKLNP
                     QHLVISPGPCTPNEAGISVAAIKHFAGRIPLLGVCLGHQSIGQAFGGRIIHAKTLMHG
                     KTSLIHHHGNSVFTGLPNPFTATRYHSLVIERETLPDCLEVTAWTDDGEIMGVRHKTL
                     AVHGVQFHPESILTEHGHDMLKNFLEGKR"
     gene            3158..4180
                     /gene="trpD"
                     /locus_tag="VFW59_04935"
     CDS             3158..4180
                     /gene="trpD"
                     /locus_tag="VFW59_04935"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5363589.1"
                     /translation="MITPQQALLRLIDQREIFYDEMLSLMRQIMRGEVTPAQIAGILI
                     GLRVKKETVGEISAAAFVMREFAAKVPVTNRMHLVDTCGTGGDAAHTFNISTASALVT
                     AAAGARVAKHGGRSVSSTSGSADVLEALGVNLNLTPEQVGQSIDAIGIGFMFAPNFHG
                     AMKYAAPVRRELGVRTIFNVLGPLTNPAGAENQVLGVFHPDLVGIQARVLQRLGSRHV
                     LVVHGSDGLDEITLGGTSLIAELKNGEISEYTVCPEDFGLQSTSLDAVRVGDGQAAKA
                     MLLTVLDNQAGPARDIVQLNAGAAIYVAGLASTLAEGIARAGEIIASGAAKAKLEQLI
                     QFSNKH"
     gene            complement(4177..5454)
                     /locus_tag="VFW59_04940"
     CDS             complement(4177..5454)
                     /locus_tag="VFW59_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5363590.1"
                     /translation="MSTPSFQSDLRHVIYALSDALDLVGIDDVAHGKRVGIMAAECGK
                     VLGLAEAETTFLFDLGMLHDIGVSTTQTHQHLIEQFDWEGSQVHAELGYGLLKKFVPL
                     SHMAIPVRYHHTRWEKLIALPTSSLTAREALQANLIFMVDRVDALAAPYYGSNALFDH
                     LDEIREHIRQNSGTYFSPELVASFMAASEAEAFWLLLEPRSIQNYLVDLLSRGTSYQP
                     SFAELKQLAVIFSQIVDAKSPFTAYHSLGVARLSRFLAECAGVSEENCDKLEIAGLLH
                     DLGKLRVPDEILDKPGQLDAHERLVINTHSFETYQILRGIGGFEEIALWAAYHHEEPD
                     GMGYPFRVREKDLSIEARILRVADIFQAMAQDRPYRAGWPAHEVLGLLKNMAGKGSLD
                     AAIVGLVEQNMAQAMAVALPGIDELPFGLSAAA"
     gene            complement(5666..>5897)
                     /locus_tag="VFW59_04945"
     CDS             complement(5666..>5897)
                     /locus_tag="VFW59_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX5363591.1"
                     /translation="LATILTTFMDAGQLRKADPDLAAAQLRALLEAEVFEPLLLQALD
                     TPPNDRALKKAAARAVDTFLRAYAPESGAVQG"
BASE COUNT         1216 a         1863 c         1803 g         1015 t
ORIGIN      
        1 ctacgcgctg gcgcgcgagc ggctgcgcga actcatcggc atgatgcggc tgcccgtcga
       61 gataccctat gcgccggcca caccgcgcgt cgaggccaag tccgaattcg gcgaagccgc
      121 gttcaagcag gcggtggaga aggccaagca gtacatattc gacggcgaca tcatgcaggt
      181 ggtgctggcg cagcgcatgt cgcagccctt ccccgcctcg ccgctgtcgc tgtaccgcgc
      241 gctgcgcagc atcaatccct cgccctacct gttctattac gacatgggcg atcaccatgt
      301 ggtcggatct tcgccggaga ttctggcgcg actcgaaggc gacaccgtca cggtgcgccc
      361 gatcgccgga acccgtccgc gcggcaagac accgcagcag gatgcggcct tggcagatga
      421 gttgttggcc gacccgaagg aactggccga acacctgatg ctgatcgacc tggggcgcaa
      481 cgacatcggc cgcgtggccg agaacggcac ggtcaggctc accgacaaga tggtgatcga
      541 gcgctattcg catgtgatgc atatcgtctc caacgtcgag gccaaactca agcagggact
      601 ggatgcgatg gatgtgctca aagcaacgtt ccccgccggc acggtgtcgg gtgcggccaa
      661 ggtgcgcgcg atggagatca tcgacgaact ggagccttcc aaacgcggca tctacgcggg
      721 cgcagtcggc tacctcggct tcaacggcga catggatgta gcgatcgcgc tgcgcaccgc
      781 cgtggtgaaa gacaagatgc tgtacgtgca ggcgggtgcg ggcatcgtcg ccgactcggt
      841 gcccgagaac gaatggatcg agacgcagaa caaggcccgc gccgtgctgc gcgcagccga
      901 gatggtgctg gacggactgg acgcgcaggc gcatcgctag accatgctgc gcctgaccga
      961 aatcaaactg cccatcaccc accccgaggg cgacatccgg acagccatcc tcaagcgtct
     1021 ggacatcgcc gatgccgaac tgatccgcta catcgtgttc aagcgcggcg tggatgcgcg
     1081 caagaagaac ggcatcctgt tcgcctacac gctggacgtc gaagtacgcg acgaggcgga
     1141 agtactcgcg cgtttcaaga atgacccgca tgtcggcatc acgcccgaca ccagttatca
     1201 cttcgtaaca caggccccgc agtctctggc cgaacgcccc gtcgtgatcg gcatgggccc
     1261 ggccggacta ttcgccggac tggtgctcgc gcagatgggt ttccgtccgc tgatactgga
     1321 gcgcggcaag gccgtgcgcg agcgcaccaa agacacctgg ggactgtggc gccagggcaa
     1381 gctcgatccc gaatccaacg tgcagttcgg cgagggcggg gccggcacct tctcggacgg
     1441 caagctgtac agccaggtca aggatccgcg attcctgagc cgcaaggtgc tggacgagtt
     1501 cgtcaaggca ggtgcgccgg atgaaatcct gtatgagagc catccgcaca tcggcacctt
     1561 ccgtctggtc ggcatggtgg agaagatgcg cgagaccatc gcgtcgctgg gcggcgagat
     1621 ccgcttccag agccgcgtgg acgacatcga gatcgagaat ggagaaattc gcggtgtggt
     1681 gctgagcaac ggcgaacgca tcgccacgaa ccatctggtg ctggccatcg gccacagcgc
     1741 gcgcgacacc ttcgagatgc tgcacaagcg cggcgtatac atcgaagcca agcctttctc
     1801 gatcggcttc cgcatcgagc atccgcagtc gctgatcgac cgtgcgcgct acggcagcaa
     1861 cgcgggcaac ccgctgctgg gtgcggccga ttacaagctg gtgcatcacg cccgcaatgg
     1921 ccgctcggtc tacagcttct gcctgtgccc cggcggcacg gtggtcgccg cgacctccga
     1981 accggggcga gtggtcacca acggcatgag ccagtattcg cgcaacgaac gcaacgcaaa
     2041 ctcggggatc gtcgtgggca tctcgccgga agactttccg gtcgacccgg aaaatccgga
     2101 caacccgctg gccggggtgg aactgcaacg ccgctgggaa tcgcgcgcat tcgaactggg
     2161 cggcggcacc tatcaggcac cggggcaact ggtcggcgac ttcctcgcag gcaaaccctc
     2221 gacccaattc ggcgaagtgc aaccctccta cacgccgggc gtgcatctga ccgacctgtc
     2281 caccgcgctg cccgactacg cgatcgcagc gatccgcgag gcgctgcccg catttgccaa
     2341 acagatcaga ggattcgatc tgagcgacgc ggtgttgacc ggcgtagaga cacgcacctc
     2401 gtcgcctgtg cgcatcaagc gccgcgacga cgacctgcaa agcatcaaca cgcgcggcct
     2461 gtatccgagc ggcgagggtg cgggctatgc gggcggcatt ctgtccgctg cgatcgatgg
     2521 catcaagatc gccgaagccg tggcattgaa catgatgcac gcgaaaaatt gagactggag
     2581 ccaacatgct tttgatgatc gataactacg attccttcac ctacaatctg gtgcagtatt
     2641 tcgccgaact cggcgcggat gtagtggtgc accgcaacga cgagatcacc gtggagcaga
     2701 tcgaaaaact gaatccgcaa catctggtga tttcgcccgg cccctgcacg ccgaacgagg
     2761 caggcatctc ggtcgcggcg atcaaacact tcgcgggcag gattccgctg ctcggcgtgt
     2821 gcctcggcca ccagagcatc ggccaggctt tcggcggcag gatcatccac gccaagacgc
     2881 tgatgcacgg caagacctcg ctgatccatc accacggcaa cagcgtgttc accggcttgc
     2941 ccaatccctt caccgcgacg cgctatcact cgctggtaat cgagcgcgag accctgccgg
     3001 attgtctgga ggtcaccgca tggacagacg acggcgagat catgggcgtg cgtcacaaga
     3061 cgctggcggt gcacggcgtg cagttccatc cggaatcgat cctgaccgaa cacggccacg
     3121 atatgctgaa aaactttctt gaagggaaga gatagccatg atcacaccac agcaggcact
     3181 gctgcgtctc atcgatcagc gtgagatctt ctacgacgag atgttgtcgc tgatgcgtca
     3241 gatcatgagg ggagaagtga cccctgcgca gatcgccggc attctgatcg gactacgcgt
     3301 caagaaagaa acggtagggg agatttccgc cgccgccttc gtgatgcgcg agttcgccgc
     3361 caaggtgccc gtcaccaacc gcatgcatct ggtagacacc tgcggcaccg gcggcgacgc
     3421 cgcccacacc ttcaatattt ccaccgccag tgcgctggtc acggcggcgg ccggcgcgcg
     3481 cgtcgccaaa cacggcgggc gctcggtttc cagcaccagc ggttcggccg acgtgctcga
     3541 ggcgctgggc gtcaatctca acctgacgcc agaacaggtc gggcagagca tcgacgcaat
     3601 cggcatcggc ttcatgttcg cgccgaactt tcacggcgcg atgaaatatg ccgcgccggt
     3661 gcgccgcgaa ctcggcgtgc gcacgatctt caacgtgctg gggccgttga ccaatcctgc
     3721 cggagcagag aatcaggtgc tcggcgtgtt ccaccccgat ctggtcggca ttcaggcgcg
     3781 cgtactgcaa cgcctcggca gccgccatgt gctggtggtg cacggctcgg atggcctgga
     3841 cgagatcacg ctgggcggca ccagcctgat cgcagagctg aaaaacggcg agatcagcga
     3901 atacaccgtg tgtccggaag atttcggcct gcaatccacc tcgctcgacg ccgttcgtgt
     3961 cggagacggg caggcggcga aggcgatgct gctgaccgtg ctcgacaacc aggcaggtcc
     4021 tgcgcgcgac atcgtgcaat tgaatgccgg tgcggcgatc tatgtggcag gattagcttc
     4081 aacgctggcc gaaggcatcg cacgggccgg cgagatcatc gccagcggtg cggccaaagc
     4141 caagctggaa caactgatcc agttcagcaa caagcactag gctgcagcgc tcagaccgaa
     4201 cggcagctca tcgataccgg gcagtgccac cgccatcgcc tgagccatgt tctgctcgac
     4261 cagcccgacg atggcggcat ccagactgcc cttgcctgcc atgttcttga gcaatcccaa
     4321 tacctcgtgc gcgggccagc cggcccggta ggggcgatcc tgagccatgg cctgaaagat
     4381 atccgcgacg cgcagaatgc gcgcctcgat cgacaaatcc ttctcgcgca cccggaacgg
     4441 ataacccata ccgtccggct cctcgtgatg gtaggccgcc cacaacgcga tttcctcgaa
     4501 gcccccgatg ccgcgcagta tctgataggt ctcgaagctg tgggtattga tgaccagccg
     4561 ctcatgcgca tccagctgcc cgggcttgtc cagtatctcg tccggcacgc gcaacttgcc
     4621 gagatcgtgc aacaaccccg cgatctccag tttgtcgcaa ttctcctcgc tcactcccgc
     4681 gcattcggcg aggaaacgag acaggcgcgc cacacccagc gagtgatagg cggtgaacgg
     4741 actcttggca tcgacgatct gggaaaagat caccgccagc tgtttgagtt cggcaaatga
     4801 gggttgatag cttgtgccgc gcgacaacag atcgaccaga taattctgga tcgagcgcgg
     4861 ctccagcaac agccagaatg cctcggcctc ggaggccgcc atgaaggagg ccaccagctc
     4921 gggagaaaaa taagttccgg aattttgtcg aatatgctca cgtatttcgt ctagatgatc
     4981 gaacagcgca ttgctgccgt agtagggtgc agccagcgca tccacccgat cgaccatgaa
     5041 gatgagattc gcctgcagcg cctcgcgcgc cgtcagactg gaggtcggca acgcaatcag
     5101 tttttcccaa cgggtgtggt gataacgcac cgggatcgcc atgtgcgaca gcggcacaaa
     5161 tttcttcagc aggccatagc ccagctcggc atgcacctgc gaaccttccc agtcgaattg
     5221 ctcgatcaga tgctggtgcg tctgggtcgt ggatacgccg atgtcgtgca gcatgcccag
     5281 atcgaacaga aaggtcgtct ccgcctccgc tagcccaagc accttgccgc actcggcggc
     5341 catgatgccg acccgtttgc catgcgcaac atcatctatc cccaccagat cgagcgcatc
     5401 cgataatgca tagatgacat gacgcagatc ggactgaaag gatggagtgg acatgagcag
     5461 cctctcgggt aacgggttgg gagggagcat tctagtgcag tgttgaactc tgtttggaac
     5521 tttccctgtg ggaacggcta tcagtcgcga tagtgcttgt taattcagcg ccccatcgcg
     5581 gctaaagccg ctcctacaaa aacttcaatc aggtttcatc aacagtgcga caccaacggc
     5641 gcaagccacc tcccgcagtg cgctgctatc cctgcacagc gccgctctcc ggcgcataag
     5701 ccctgaggaa agtatcgacg gcacgggcgg cggccttttt gagcgcgcgg tcgtttgggg
     5761 gggtatccag cgcctggagc agcagcggct cgaagacttc cgcttccagc agggcgcgca
     5821 actgcgctgc ggccagatct ggatcggcct tgcgcaattg ccccgcgtcc atgaaggtcg
     5881 taaggatggt cgccaac
//
LOCUS       DASUIV010000131         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_866751, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000131 DASUIV010000000
VERSION     DASUIV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866751"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFW59_04950"
     CDS             <1..895
                     /locus_tag="VFW59_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite reductase, dissimilatory-type subunit
                     alpha"
                     /protein_id="HEX5363592.1"
                     /translation="QGATTENVQKAFDELNEMGFDLGGAGPAVRTSMSCVGAARCEQS
                     CYDEAKTHRAVINTFLDDIHRPSLPYKFKFKFSGCPNDCMNSVQRADMAVIGTWRDNI
                     RTDEALAKKWFAKHGMDELVNDVVARCPTKTFQLKEIKNVKKSDSISSVAVSDTHAVE
                     IDNRDCVRCMHCINVMTGALAPGKDKGATVLVGGKSHLKIGGLLGTVIIPFIKLDTDE
                     GVEKLVDFAQRTIDFFAENALEHERTGEMIERIGLANFLEAMEIDADPAMINSPRMNP
                     YVRTDGWDDEVAKINAKKQAV"
     gene            907..1986
                     /gene="dsrB"
                     /locus_tag="VFW59_04955"
     CDS             907..1986
                     /gene="dsrB"
                     /locus_tag="VFW59_04955"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HEX5363593.1"
                     /translation="MNAPVQMRKPKETGVPDNKKYLHPLFAKNYGDWKYHDRPRPGVL
                     HHVAHSGDEVWSVRAGTQRQMDVHTIRRLCDIADKFAEGRVRFTIRSNIEFMVSEESK
                     VAPLIDALEKGGFPVGGTGNSVTMIAHTQGWLHCDIPGTDASGVVKALMDELYDEFRR
                     EEMPNRVHLSTSCCEINCGGQADLAIIVQHTKPPVINHDLVANVCERPTVVARCPVAA
                     IRPAMVNGKPSLEIDESKCMCCGACYPPCPPMQINDPENSKLAIWVGGKNSNARGKPT
                     FMKMVASGLPNNAPRWPEVSAAVKNILHVYKEDARPWERMADWIDRIGWPRFFEKTGL
                     PFTKYLIDDWRGSRANLNSSTHIRF"
     gene            2000..2359
                     /gene="tusD"
                     /locus_tag="VFW59_04960"
     CDS             2000..2359
                     /gene="tusD"
                     /locus_tag="VFW59_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HEX5363594.1"
                     /translation="MKFGIVVNEGPYQHQASDSAYLFAKAAIEKGHEVWRVFFYHDGV
                     NNASKLTEPPQDDRHIVNRWTKLAEEHKVDLVVCVAAALRRGIKEENLAAGFRISGLG
                     QLVEAGVKCDRLLTFGD"
     gene            2370..2735
                     /gene="tusC"
                     /locus_tag="VFW59_04965"
     CDS             2370..2735
                     /gene="tusC"
                     /locus_tag="VFW59_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HEX5363595.1"
                     /translation="MSDVKKFMFVNRKTPHGTIYALESLEVVLITAAFDQDVSLVFMD
                     DGVYQLKKGQVTKGIETKNFSPAYRALEGYDIEKLYVEKESMEARGLTEADLLVDVTV
                     LSRAEMGELMDQQDVVLSF"
     gene            2808..3104
                     /gene="tusB"
                     /locus_tag="VFW59_04970"
     CDS             2808..3104
                     /gene="tusB"
                     /locus_tag="VFW59_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HEX5363596.1"
                     /translation="MLHIINKSPLTNAALDSCLRVAQSGDILLIEDAVVAAASGNAYE
                     GKLREAMGRCKVYVLQPDLEARGLADRLIEGVSPVDYGGFVDLATTNKNCQSWL"
     gene            3129..3464
                     /locus_tag="VFW59_04975"
     CDS             3129..3464
                     /locus_tag="VFW59_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX5363597.1"
                     /translation="MSSIEVNGKSYETDEEGYLLDLGEWNKEVAEYIAGTESIAMTEQ
                     HWEVINFLREYFEEYQIAPAVRVLTKAIGKKLGADKGTSEYLYGLFPYGPGKQACKIA
                     GLPKPTGCV"
     gene            3567..4292
                     /locus_tag="VFW59_04980"
     CDS             3567..4292
                     /locus_tag="VFW59_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX5363598.1"
                     /translation="MSTLFAILFYLATLLFVVGLVYRINDYARTPAPLKIPTTPAPTT
                     TGGVVLRMTREVVFFESLFKANLWTWALGWLFHASMALVVLRHLRYFTEPVWGWVEFI
                     QPFGIYAGITMLLGLAGLWARRLFVERIRYISTPSDHLMLVLLIAIAASGLLMKFAAH
                     ADVIGVKGFFLGLMHFSLQPLPTDALLIVHLSLVLLLLAIFPISKLLHAPGVFFSPTR
                     NQTDNPREKRHISAWAKDLESDK"
BASE COUNT          905 a         1250 c         1325 g          870 t
ORIGIN      
        1 ccagggcgcg accaccgaga atgtgcagaa ggcgttcgac gagctcaacg agatgggttt
       61 cgatctcggc ggcgcgggtc ctgcggtgcg tacctcgatg tcctgcgtgg gcgccgcgcg
      121 ttgcgagcag tcctgctacg acgaagcgaa gacccatcgt gcggtgatca acactttcct
      181 ggacgatatc caccgtccat cgctgcctta caagttcaag ttcaagttct ccgggtgccc
      241 gaacgactgc atgaactcgg tgcaacgcgc cgacatggcg gtaatcggca cttggcgcga
      301 caacatccgc accgacgagg cgctggcgaa gaagtggttc gccaagcacg gcatggacga
      361 gctggtgaac gacgtggtgg cacgctgccc gaccaagacc ttccagctca aggaaatcaa
      421 gaacgtcaag aagagcgaca gcatctccag cgtggcggtc agcgacaccc atgcggtcga
      481 gatcgacaac cgcgactgcg tacgctgcat gcactgcatc aacgtgatga ccggcgcgct
      541 ggctccgggc aaggacaagg gcgcgactgt gctggtgggc ggcaagagcc atctgaagat
      601 tggcggcctg ttgggcaccg tgatcattcc gttcatcaag ctggataccg acgagggtgt
      661 cgagaagctg gtcgatttcg cgcagcgcac catcgacttc ttcgccgaaa atgcgctgga
      721 gcacgagcgt accggcgaga tgatcgagcg tatcggtctg gccaacttcc tcgaggcgat
      781 ggagatcgat gcggatccgg cgatgatcaa ttcgccgcgc atgaacccct atgtccgtac
      841 cgacggctgg gatgacgaag tcgccaagat caacgctaag aaacaagcag tctgaggaga
      901 taaaacatga atgcacccgt gcaaatgcgc aagccgaaag agactggcgt acccgataac
      961 aagaagtatc tgcacccgct gtttgcgaag aactacggcg actggaaata ccacgaccgt
     1021 ccgcgtcccg gcgtgctgca tcacgtggcg cacagcggcg acgaagtttg gtcggtgcgt
     1081 gccggcacgc agcgtcagat ggacgtgcac accattcgca gactgtgcga catcgccgac
     1141 aagttcgccg aaggccgcgt gcgtttcacc atccgttcca acatcgaatt catggtgtcg
     1201 gaggagagca aggttgcgcc gctgatcgat gcgctggaaa agggcggctt cccggtcggc
     1261 ggtaccggca actcggtgac catgatcgcg cacacccagg gttggttgca ttgcgacatc
     1321 ccgggtaccg acgcttccgg cgtggtgaag gcgctgatgg acgagttgta cgacgagttc
     1381 cgccgcgaag agatgccgaa ccgtgtgcac ctgtccactt cctgctgcga gatcaactgc
     1441 ggcggccagg ccgacttggc gatcatcgtg cagcacacca agccgccggt catcaatcat
     1501 gatctggtcg ccaacgtctg cgagcgtccg accgtggttg cgcgctgccc ggtcgcggcg
     1561 atccgtcccg cgatggtgaa cggcaagcct tcgctggaga tcgacgagtc caagtgtatg
     1621 tgctgcggcg cttgctaccc tccctgcccg ccgatgcaga tcaacgatcc ggagaactct
     1681 aagctggcga tctgggtggg cggcaagaac tccaacgcgc gcggcaagcc gaccttcatg
     1741 aagatggtcg catcgggtct gccgaacaat gcgccgcgct ggcccgaagt ttctgctgcg
     1801 gtgaagaaca tcctgcatgt ttacaaggaa gatgcgcgtc cttgggagcg catggccgac
     1861 tggatcgacc gtatcggctg gccgcgtttc ttcgagaaga ccgggctgcc gttcaccaag
     1921 tacctgattg acgattggcg cggttcgcgt gccaacctca actcttcgac ccacatccgt
     1981 ttctgatccg ggggctagca tgaagtttgg aatcgtagtc aacgaggggc cgtaccagca
     2041 tcaggcgagc gattcggctt acctgttcgc caaggcggcc atcgagaagg ggcacgaggt
     2101 gtggcgcgtg ttcttctatc acgacggcgt caacaacgcg tcgaagctga ccgagccgcc
     2161 gcaggacgac cgtcatatcg tgaaccgctg gaccaagctg gccgaggaac acaaggtcga
     2221 tctggtggtc tgcgtggcgg ctgcgctgcg tcgcggcatc aaggaagaga atctggcggc
     2281 gggctttcgc atttccggtc tgggtcaact agtcgaggcg ggcgtgaagt gcgaccgtct
     2341 gctgaccttc ggcgattaag gagagcgaaa tgagtgatgt gaaaaaattc atgttcgtga
     2401 accgcaagac gccccacggc acgatctacg cgcttgagtc gctggaggtg gtgttgatca
     2461 cggcagcctt cgatcaggac gtgtcgcttg tgttcatgga cgacggcgtc tatcagctga
     2521 aaaaaggtca ggtgaccaag gggatcgaga ccaagaactt ttcgcccgcc taccgtgcgc
     2581 tggaaggtta cgacatcgag aagctctatg tcgaaaagga atcgatggag gcgcgcggcc
     2641 tgaccgaggc cgatctgctg gtggatgtca ccgtgctgag ccgtgccgag atgggcgagt
     2701 tgatggacca gcaagacgtg gtcctgagtt tctgatgaaa cgactagcca ttcgactagg
     2761 ctgtccaact acgccagcca agtcgctggt tataaggagg aggttaaatg ctgcatatca
     2821 tcaataaatc cccgctgacc aatgccgcgc tggacagctg tctgcgcgtt gcccagtccg
     2881 gcgacatcct gctgatcgag gacgcggtgg ttgccgccgc gtccggcaat gcctacgagg
     2941 gcaagctgcg cgaggcgatg ggccgttgca aggtctacgt cctgcaaccc gacctggagg
     3001 cgcgcggcct ggccgatcgt ctcatcgaag gtgtttcacc tgtggattac ggcggtttcg
     3061 tcgatctcgc cacgaccaat aaaaactgcc aatcctggct ctaacctgta ctgaaaagga
     3121 gaaatacgat gtctagcatt gaagttaacg gcaagtctta cgaaaccgac gaagaaggtt
     3181 atctgctgga tctgggcgag tggaataaag aagtggcgga gtacatcgcc ggtacggaga
     3241 gcatcgcgat gaccgaacag cactgggagg tgatcaactt cctgcgcgaa tatttcgagg
     3301 aataccagat cgctccggca gtgcgcgtgt tgaccaaggc gatcggcaag aagctgggcg
     3361 cggacaaggg taccagcgag tatctgtacg gtctgttccc gtacggcccg ggcaagcaag
     3421 cctgcaagat cgccggcctg cctaagccga ccggttgcgt gtaagacgca ggattgagga
     3481 tcgaggattg gggatcgagc ctggctcgcc tcaatccgag agtggctcaa tcctaattcc
     3541 tcaatcctaa ttcttgaatc cagacaatga gtacactgtt cgcaatcctg ttttaccttg
     3601 caaccttgtt gtttgtcgtg ggattggtct accgcatcaa tgactacgcg cggacgcccg
     3661 cgccgctgaa gatccccact actcccgctc cgaccactac cggcggcgtg gtgctgcgca
     3721 tgacgcgcga agtggtgttt tttgaaagtc tgttcaaagc gaatttatgg acctgggcgc
     3781 tgggctggct gtttcatgcc agcatggcac tggtcgtact gcgccatttg cgctatttca
     3841 ccgagcctgt ctggggctgg gtcgaattca tccagccgtt cggcatctat gcgggcatca
     3901 ccatgctgct cggtctcgcc ggactgtggg cgcgccgcct gtttgtcgag cgcatccgtt
     3961 acatctctac tccctctgac catctgatgc tggtgctgct catcgccatc gccgcttccg
     4021 gcctgctgat gaagtttgcg gcccatgccg acgtgatcgg ggtgaagggt ttcttcctgg
     4081 ggctgatgca cttcagcctt cagcctctgc ccacagatgc gctgctgatc gtgcatctgt
     4141 cgctggtttt gctactcctg gctatcttcc cgataagcaa gctcctccat gcgccagggg
     4201 tctttttctc tcccacccgc aatcaaacgg ataacccgcg cgagaaacgc catatttccg
     4261 cctgggctaa agatctggag tcagataaat gagtgcaccg actaccgctc cggcctatcg
     4321 catcatcccc atcatcccgg ccctgctgcc
//
LOCUS       DASUIV010000132         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1240763, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000132 DASUIV010000000
VERSION     DASUIV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240763"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1245
                     /locus_tag="VFW59_04985"
     CDS             79..1245
                     /locus_tag="VFW59_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX5363599.1"
                     /translation="MKIAIAGTGYVGLSNAILLAQHHEVVAVDIVPEKIAMLNRKQSP
                     IEDTEINDYLRHKTLNFKATLDKVEAYSGADYVIIATPTDYDPETNYFNTKSIESVIQ
                     DVLAINPQAVMVIKSTIPVGYTVKTRAKFNCDNLIFSPEFLREGRALHDNLHPSRIVV
                     GEQSRRAEVFADLLKQGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ASHGLDTKQIIQGVCLDPRIGEHYNNPSFGYGGYCLPKDTKQLLANYQNVPQNLINAI
                     VASNTTRKDFVAESILKHNPKVVGIHRLVMKSGSDNFRSSSIQGIMKRIKAKGVEVIV
                     YEPALKEAEFFHSRVVNDLAQFKRQADIIVANRITDDIRDVVDKVYSRDLFGKD"
     gene            complement(1304..2155)
                     /locus_tag="VFW59_04990"
     CDS             complement(1304..2155)
                     /locus_tag="VFW59_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5363600.1"
                     /translation="MSEARGLEYWVAILTRAELPVLKQTARALNELRADEEKLTASGV
                     ADIIARDPIMTVKLLRYLQQHKHKIQTTEVIQVEQALLMMGIEPVFRKVPPEPLVEDA
                     LIQKTPALLQMLHTVRRSHLASEYAKDWAVRLRDLHYEEVRIAALLHDIAEILMWCFA
                     PDQMLEIEEIKRRERMVRTRTIQEQVFGFSLYQLQHELAVQWELPQLLLTLMNDSCAH
                     MPRVRNVVLAVNLVRHSANGWDDPALPDDYKDIAELLRMPVKDVVDMIAVDAGIACDI
                     HQPNRGD"
     gene            complement(2252..3148)
                     /locus_tag="VFW59_04995"
     CDS             complement(2252..3148)
                     /locus_tag="VFW59_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5363601.1"
                     /translation="MSKQLALKIDVDTYRGTREGVPRLVDALQRYQAQATFFFTLGPD
                     HMGRAIKRVFRPGYLSKVSRMSVVEHYGIKTLLYGTLLPAPDIGGKCADIMRSVRDAG
                     FETGVHCYDHIRWQDQVADKDEVWTRKELMRAVDRYTEIFGEAPRAHAAAGWQMNRHA
                     LRMMQHLGFAYSSDSRGTQPCIPCWNAEIVNCPQLPTTLPTLDELINRDGITLDNIAA
                     HVLQLTAVEPLAGHVFTLHAELEGGKWMPVFEQLLAGWQAQGYELVSLRQYLAQLTVS
                     DLPRKDMTLREVDGRIGKLAVV"
     gene            complement(3223..4263)
                     /locus_tag="VFW59_05000"
     CDS             complement(3223..4263)
                     /locus_tag="VFW59_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEX5363602.1"
                     /translation="MKKVLILGVNGFIGHHLSNRILATTDWEVYGMDMSTDRISDLIG
                     QPRFHFFEGDITINKEWVEYHVKKCDVILPLVAIATPSTYVKQPLRVFELDFEANLPI
                     VRAAVKYNKHLVFPSTSEVYGMCHDEEFDPAESELVCGPINKPRWIYSCSKQLMDRVI
                     WGYGMEGLNFTLFRPFNWIGAGLDSIHTPKEGSSRVVTQFLGHIVRGENISLVDGGHQ
                     KRAFTYIDDGIDALMKIIANKDGIATGKIYNIGNPTNNHAIRDLADMMLKLAGEYAEY
                     RDSAAKVKIVETTSAAYYGKGYQDVQNRVPKITNTCEELGWKPTTTMADTLRNIFEAY
                     REQIAEAKGLVD"
     gene            complement(4273..>4764)
                     /locus_tag="VFW59_05005"
     CDS             complement(4273..>4764)
                     /locus_tag="VFW59_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEX5363603.1"
                     /translation="IVAQQAVPILPNDTAHEVFQKVTVAAEIALNNVLPMLLSGKAQA
                     VKQDLSQGGYFGGRRAEDGVIAWSQSAQQIHNLVRAVAPPYPGATTVLMGKPMRILQT
                     LVTKCTVAGKMPPAFYVKDGRAYAICGSGVLRVVRFELDGTEMSAAEFAAQHGSEKFE
                     FNC"
BASE COUNT          930 a         1523 c         1375 g          936 t
ORIGIN      
        1 ttcgatcaga ctgccccgct gacgccgccc cctgttctgc ggcataatgc acgcctcgtt
       61 ttttgaacta ctgatgcaat gaaaatcgcc atcgcgggaa ccggctacgt cggactttcc
      121 aacgccatcc tgctcgcaca gcaccatgaa gtcgtcgcgg tggacatcgt ccccgaaaag
      181 atcgcgatgc tgaaccgcaa gcagtcgccc atcgaagaca ccgagatcaa cgactatctc
      241 cggcacaaga cgctcaactt caaggccacg ctggacaagg tcgaggccta cagcggtgcg
      301 gattacgtca tcatcgccac gccgaccgac tacgatccgg aaaccaatta cttcaacaca
      361 aagtcgatcg agtccgtcat tcaggatgtg ctcgccatca acccgcaggc ggtgatggtg
      421 atcaagtcga ccatccccgt cggctatacg gtcaagaccc gcgccaagtt caattgcgac
      481 aacctgatct tctcgcccga attcctgcgc gaaggacgtg cgctgcacga caacctgcac
      541 ccctcgcgca tcgtggtcgg cgaacaaagc agacgcgccg aggtttttgc cgacctgctc
      601 aaacagggtg cgatcaaaca ggacatcccc acgctgttca ccgactcgac cgaggccgag
      661 gcgatcaagc tgttcgccaa cacctacctc gcgatgcgcg tggcctactt caacgaactc
      721 gattcctacg ccgcctcgca cggcctcgat accaaacaga tcatccaggg cgtctgcctc
      781 gacccgcgca tcggcgaaca ctacaacaac ccctcgttcg gctacggcgg ctactgcctg
      841 cccaaggaca ccaagcaact gctggccaat tatcagaacg tgccgcagaa cctgatcaac
      901 gcgatcgtgg cgtccaatac cacgcgcaag gacttcgtcg ccgaaagcat cctcaagcac
      961 aacccgaagg tggtcggcat ccaccgcctg gtgatgaaga gcggctccga caacttcaga
     1021 tcgagcagca tccagggcat catgaagcgc atcaaggcca agggcgtcga ggtcatcgtg
     1081 tacgagcccg cattgaagga ggcggagttc tttcactccc gcgtggtgaa cgacctggcg
     1141 caattcaagc ggcaggcgga catcatcgtc gccaaccgca tcaccgacga catccgcgat
     1201 gtggtcgaca aggtttacag ccgggatttg ttcggcaagg actaagggca tgccccgcag
     1261 gcgatgagct ggcgacagcg ctccgcccag gagccgatcg gagtcagtcg ccccggtttg
     1321 gctggtggat gtcgcaggcg atgccggcat cgaccgcgat catgtccacc acatccttga
     1381 ccggcatgcg cagcagctcg gcgatgtcct tgtagtcgtc cggcagcgcg ggatcgtccc
     1441 agccattggc cgaatgccgc accagattga ccgccagcac cacgttgcgc acgcgcggca
     1501 tgtgcgcgca ggaatcgttc atcagcgtca gcagcagttg cggcagctcc cattgcaccg
     1561 ccagttcgtg ctgcaactga tagaggctga agccgaacac ctgctcctgt atggtacggg
     1621 tgcgcaccat gcgctccctg cgcttgatct cttcgatttc cagcatctga tcgggcgcga
     1681 agcaccacat caatatctcg gcgatgtcgt gcaacagcgc cgcgatgcgc acctcttcgt
     1741 agtgcagatc gcgcaggcgt acggcccagt ctttggcgta ttcggaagcg agatgggaac
     1801 gacggaccgt gtgcaacatc tgcaacaacg cgggggtctt ttgtatcagc gcatcctcga
     1861 ccagcggctc gggaggcacc ttgcggaaca ccggctcgat gcccatcatc agcaaggcct
     1921 gctccacctg gatcacctcg gtggtctgta tcttgtgttt gtgctgctgc agataacgca
     1981 gcaatttgac ggtcatgatc gggtcgcgcg cgatgatatc ggcgaccccg ctggcagtca
     2041 gcttctcctc atcggcgcgc aattcgttca gcgcgcgcgc ggtctgcttg agcaccggca
     2101 gttccgcgcg cgtgagtatc gcaacccaat attccagccc ccgtgcttcc gacattgcct
     2161 ctccctcgcc tagatcctgt ttaaacaggc tgttattgcc cgcgaatata cccgcattcg
     2221 cgcgacttgc acaatctgtg cggaagagct gtcagaccac cgccagcttg ccgatgcggc
     2281 cgtcgacctc gcgcagcgtc atgtccttgc gcggcaaatc ggaaacagtc agctgcgcga
     2341 gatactgacg cagcgacacc agctcgtagc cttgtgcctg ccagcccgcg agcagttgct
     2401 cgaacaccgg catccatttg ccgccttcga gttcggcatg cagcgtgaat acatgaccgg
     2461 cgaggggttc gacggcagtc agttgcaaca catgcgcggc gatgttatcc agcgtgatgc
     2521 cgtcgcgatt gatgagttca tccagcgtcg gcaaggtggt cggcagttgc gggcagttca
     2581 cgatctcggc attccagcag gggatgcagg gctgcgtgcc gcgcgagtcg gaactgtagg
     2641 cgaagcccag atgctgcatc atgcgcaagg catggcggtt catctgccag ccagctgcgg
     2701 catgggcgcg cggcgcctcg ccgaatattt cggtgtagcg gtccaccgca cgcatcaatt
     2761 ccttgcgggt ccacacctcg tccttgtccg ccacctgatc ctgccagcgg atatggtcgt
     2821 agcaatgcac gccggtctcg aagcccgcat cgcgcaccga gcgcatgatg tcggcgcatt
     2881 tcccgccgat gtccggcgcg ggcaacagcg tgccgtacag cagggtcttg atgccgtagt
     2941 gttccaccac cgacatgcgc gacaccttgg acagatagcc cggacggaac acccgcttga
     3001 tcgcccgccc catgtgatcg ggacctagcg tgaagaagaa ggtcgcctgc gcctggtagc
     3061 gctgcaacgc atcgaccaga cgcggcaccc cttcgcgcgt gccgcgatag gtgtcgacgt
     3121 cgatcttgag agcgagttgt ttagacattg cgagtcccct ctcccccagc ccctctcccg
     3181 cgagcgggag aggggtgcgc gtcagcgcgg gagagggatt gattagtcga ccagaccctt
     3241 ggcttcggcg atctgctcgc ggtaggcttc gaagatgttg cgcagggtgt cggccatggt
     3301 ggtggtcggt ttccagccca gctcttcgca ggtgttggtg atcttcggca cgcggttctg
     3361 cacgtcctga taacccttgc cgtaataagc ggcagaggtg gtctcgacga tcttcacctt
     3421 ggctgccgaa tcgcggtatt cggcatactc gcccgccagc ttgagcatca tgtcggccag
     3481 atcgcggatc gcgtggttgt tggtcgggtt gccgatgttg tagatcttgc cggttgcgat
     3541 gccgtccttg ttggcgatga tcttcatcag cgcgtcgatg ccgtcgtcga tataggtgaa
     3601 cgcgcgtttc tgatgcccgc cgtcgaccag actgatgttc tcgccgcgca cgatgtgacc
     3661 gaggaactgg gtcaccacgc gcgagctgcc ttctttaggg gtgtggatgg aatccagtcc
     3721 ggcaccgatc cagttgaacg gacggaacag cgtgaagttc aggccttcca tgccgtagcc
     3781 ccagatcacg cgatccatca gctgcttgga gcaggagtag atccagcgcg gcttgttgat
     3841 cgggccgcag accagctcgg actcggccgg gtcgaattcc tcgtcgtggc acatgccgta
     3901 gacttcggaa gtggacggga acaccagatg cttgttgtac ttcacggcgg cgcgcacgat
     3961 gggcaaattg gcctcgaagt ccagttcgaa cacgcgcagc ggctgcttca cataggtgga
     4021 aggcgtcgcg atggcgacca gcggcaggat cacgtcgcac ttcttgacgt ggtactcgac
     4081 ccactccttg ttgatggtga tgtcgccctc gaagaaatga aaacgcggct ggccgatcag
     4141 gtcgctgatg cggtccgtgc tcatgtccat gccgtacact tcccagtcgg tggtcgccag
     4201 gatgcggttg gacagatggt gaccgatgaa gccgttcacg ccgaggatca gtactttttt
     4261 catttcactt ccttaacaat tgaattcaaa tttttccgaa ccatgctgcg ccgcgaattc
     4321 cgccgcgctc atctcggtcc catccagctc aaaacgcacc acccgcaaca cgccgctgcc
     4381 gcagatcgca taagcacggc cgtctttcac atagaaagcc ggcggcatct ttccggccac
     4441 cgtgcatttc gtcaccagcg tctgcaggat gcgcatcggt ttgcccatca gcaccgtggt
     4501 cgcaccggga tacggcggcg ccaccgcacg caccagattg tgtatctgct gcgccgactg
     4561 cgaccaggct atgacgccgt cttcagcgcg gcgtccgccg aaatagccgc cctgactcaa
     4621 gtcctgcttc accgcctgcg ccttgcccga cagcagcatg ggcagcacgt tgttcagcgc
     4681 gatctccgcc gccaccgtca ccttctggaa cacctcatgc gcggtgtcgt tcggcaggat
     4741 gggaacggcc tgctgcgcca cgat
//
LOCUS       DASUIV010000133         6368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_217699, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000133 DASUIV010000000
VERSION     DASUIV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217699"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1799
                     /locus_tag="VFW59_05010"
     CDS             138..1799
                     /locus_tag="VFW59_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5363604.1"
                     /translation="MTIRREATYCGGGSHECSECDAVTNDTLLEVRDLGVSFGAQAPR
                     AVDGVSFTLKQGEKFALVGESGSGKSVTALSLLRLVANARSSGEVMFAGENLVSASER
                     RLRGIRGKDIAMIFQEPMTALNPLMRIGEQITEVLSLHEGMDYAPARARAIELLGHTG
                     ITEPERRFASFPHELSGGQRQRAMIAMALACRPKLLIADEPTTALDVTIRTQIMDLLD
                     DLQREYGMTILLITHDLNLVRRFADRVGVMQSGRLVELAETAQLFSAPQHPYTRRLLD
                     SRPRKMVQPLSAEQQDAPDLLRGDGLRVEFSYPVGWFKKKIFTAVDGVSLNLKRGETL
                     GIVGESGSGKSTLAMALLALQPITAGACVFDGLSMTNIRPAQLRAMRARMQVVFQDPF
                     GSLSPRLTVEEIVGEGLALHQPKMPLAERRRRIAQTLQEVGLEPDVLVRYPHEFSGGQ
                     RQRIAIARAIILEPQLLVLDEPTSALDASVQKQVLALLAGLQLKHGMSYLLISHDLEV
                     IAAMSHRIAVMQAGRIVEQGLADSVLTSPRHDYTRQLLASVSPQD"
     gene            2002..2331
                     /locus_tag="VFW59_05015"
     CDS             2002..2331
                     /locus_tag="VFW59_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX5363605.1"
                     /translation="MSTKLANVEGDVSKEKLMQDLRVVVADAEELLRATAGQAGEKVS
                     AARERIQENLASAKDRLVAAERAVVEKTKQAAKATDEYVHENPWKAVGIAAGVGLVVG
                     MLISRNR"
     gene            2335..2715
                     /locus_tag="VFW59_05020"
     CDS             2335..2715
                     /locus_tag="VFW59_05020"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5363606.1"
                     /translation="MQESPGLLDSARRLFATLLGVVTTRVELLANEWQEERLRLAEML
                     LFALFSAFCLGVGMVLLTMFLVVLFWDEHRLAVLASLSLFFFVLGAWLIGLLRSRMRQ
                     GSRLFSVSLAELNKDRASLGARDE"
     gene            2708..3007
                     /locus_tag="VFW59_05025"
     CDS             2708..3007
                     /locus_tag="VFW59_05025"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HEX5363607.1"
                     /translation="MNDRILELRQRRGELLARIAVQRGQLIEMSARCEGPLAWVDQGM
                     AVVRFLRAKPLLVAGVAALLVIRRRGVMGLVRTGWKMWKGYRFFTAMSGKLSGRE"
     gene            complement(3019..4512)
                     /locus_tag="VFW59_05030"
     CDS             complement(3019..4512)
                     /locus_tag="VFW59_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5363608.1"
                     /translation="MPLIRLIFSALLAASAVISTPFAYADEQLALSESLTPPLTLSTA
                     RNIKLELIPDSDLAMNDLWARIRGGFAMRELDSPLIAKHEHWYASRPDYVARMTDRAR
                     LYLYYITGEVERRGMPSEIALLPMIESAFNPGAYSTSKASGIWQFIPSTGKHFGMEQN
                     WWYDGRRDVISATNGALDYLQKLHDMFGDWELALAAYNWGEGAVQRAQARNRARGLPV
                     NYTSLKMPDETRNYVPKLLAVKNIIANPASFGLVLQNVPNEPYFAAVSTAQHIDVKLA
                     AQLADVSLEEFIALNPAHNRPVILQDNSDLILLPIDKVETFRANLESYDKPLVSWQAY
                     QPKKGERLDHLAPRLGLSVEKLKSVNGLSGRSNVSTGQTLLVPINGEENESDGDFTAF
                     NMHLHPSAEPAARSISHKVLKGETLARIAARYHVSLAKLKSWNDGIVKIKPGQTLVVV
                     QTAAKGKSSRMVKAPARGQRVAKRGNNRKQASIKTAKRTHIAYGGRR"
     gene            complement(4836..>6368)
                     /gene="acs"
                     /locus_tag="VFW59_05035"
     CDS             complement(4836..>6368)
                     /gene="acs"
                     /locus_tag="VFW59_05035"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX5363609.1"
                     /translation="MSIEAIVAMQACARIGAIHSVVFGGFSSKSLHERIVDAQACAVI
                     TADAQMRGGKVMPLKTIVDEALAQEGCDCVRNVIVYRRAGSSVEWNDERDIWWHELFN
                     AQPTHCEPEWVDAEHPLFILYTSGSTGKPKGVQHSSGGYLLGTMLTMQWVFDYKAGDV
                     FWCTADVGWITGHSYVAYGPLAMGATQVVFEGIPTYPDAGRFWKMIQDHKVTTFYTAP
                     TAIRSLIKLGGDLPKQYDLSSLRLLGSVGEPINPEAWMWYYNEIGQSRCPIVDTWWQT
                     ETGCHMIAPLPGVEALKPGSCTLPIPGIQIAIVDEMGEEVGMQQGGSLVIKKPFPSQI
                     RTIWGDPERFKKSYFPEEVHGFYLAGDSANRDEDGYVWIMGRNDDVLKVSGHRLGTME
                     IESALVANPLVAEAAIVGRPHDIKGEAIVAFVVLKGARPKDAAAAKQLADTLRNWVGK
                     EIGPIAKPDEIRFGDNLPKTRSGKIMRRLLRTLAKGEEITQDISTLENPAILEQLKEV
                     IR"
BASE COUNT         1134 a         1754 c         2084 g         1396 t
ORIGIN      
        1 cagggcaagg ataacctcga tgcctggtgg ataggtctcg ccgcgttcgt cgtgctgttc
       61 gtcacgctgc tgttgctggt gttcatcggc gaggcgctgc gcgatgcgtt cgatacgcgc
      121 aagtcgggag ggagataatg acaatacggc gcgaagcgac ttattgcggt ggaggctcac
      181 atgagtgtag cgaatgtgat gccgtaacca acgacacgct gctggaagtc cgcgatctcg
      241 gcgtcagttt cggcgcgcag gcgccgcgcg ccgtcgacgg cgtgtcgttc acgctgaagc
      301 agggcgagaa atttgcgctg gtcggcgaat cgggttcggg caagagcgtc acggcgctgt
      361 cgctgttgcg tctggtcgcc aatgcgcgct ccagcggcga ggtgatgttc gctggcgaga
      421 atctggtgag tgccagcgaa cggcgcttgc gcggcatacg cggcaaggac atcgcgatga
      481 tcttccagga gccgatgacc gcgctcaacc cgctgatgcg catcggcgag cagatcaccg
      541 aggtgctctc gctgcacgaa gggatggatt acgctccggc gcgcgcacgc gccatcgagc
      601 tgctcggtca caccggcatc accgagccgg aacggcgttt tgcaagcttc ccgcacgaac
      661 tttccggcgg ccagcggcag cgggcgatga tcgcgatggc actggcctgc cgcccgaaac
      721 tgctgatcgc cgacgagccg accacggcgc tggatgtgac catccgcacc cagatcatgg
      781 atctgctcga cgatttgcag cgcgaatacg gcatgaccat tttgttgatt acccatgacc
      841 tcaatctggt gcgtcgcttt gccgacaggg tcggggtgat gcagtccgga cgtctggtcg
      901 aactggccga gaccgcgcag ctattttcag cgccgcagca tccctatacc cggcgcctgc
      961 tcgacagccg cccccggaag atggtgcagc cgctgtcggc ggaacagcaa gatgcgccgg
     1021 acttgctgcg cggcgacggt ctgcgcgtcg agttctccta tccggtcggc tggttcaaga
     1081 aaaaaatctt caccgctgtc gatggcgtga gtctgaatct gaaacgcggc gagacgctgg
     1141 gcatcgtcgg cgaatccggt tccggcaagt ccacgctggc gatggcgttg ctggcactgc
     1201 agccgatcac cgccggtgct tgtgtgtttg acggactatc catgacgaat atccggccgg
     1261 cgcagctgcg cgcgatgcgc gcccggatgc aggtggtgtt tcaggatcct ttcggcagtc
     1321 tgtcgccgcg cctcacggtc gaggagatcg tcggtgaggg gctggcgttg catcagccga
     1381 agatgccgct tgccgagcga cgccggcgca tcgcacaaac gttgcaggaa gtggggctgg
     1441 aacccgatgt gctggtgcgt tatccccacg agttttccgg cggacagcgc cagcgcatcg
     1501 cgattgcgcg cgccatcatt ctcgagcccc aattgctggt gctcgatgag ccgaccagtg
     1561 cgctcgacgc ctcggtgcag aagcaggtgc tggcgctgct ggccggattg cagctcaaac
     1621 acggcatgag ttatctgctg atcagccacg atcttgaggt gattgccgcg atgtcgcacc
     1681 gcattgcggt gatgcaggcc gggcgcatcg tcgagcaggg actggccgac tctgtcctca
     1741 cctctccgcg tcacgactat acccgtcaat tgctggccag cgtttcaccg caagattgac
     1801 tgagtatgcc gggcgatcac tgtgcctgcc gacgattcgt cagatacttt gccgtagtca
     1861 tggcggagga gttcaaataa gcaatatcct agattgacgc cccgggtttt tagggctaaa
     1921 aagacaacca aggtgtctgt aatgtcatcg gctgagtagg gggttagttg cagtattttt
     1981 ccatcgttca aggagtgaat catgagcacg aaattagcta atgttgaagg tgatgtcagc
     2041 aaggaaaaac tgatgcagga tctgcgagtc gtggtggcag atgcagagga attgttgcgg
     2101 gcgacggcgg ggcaggcagg cgagaaggtc tcggcggcac gtgagcgtat tcaggaaaat
     2161 ctggcttccg ccaaggatcg tttggtggcc gcagagcgtg ccgtagtcga aaagaccaag
     2221 caggcggcca aggcgaccga tgagtatgtg catgaaaacc cctggaaggc cgttggcatc
     2281 gcagccgggg tcggtctggt ggtcgggatg ctgatttcac gtaaccgtta aacgatgcag
     2341 gaaagtccgg gtctgctgga ctcggcccga cgcctgtttg ccacactgct cggtgtggtg
     2401 acgacccggg ttgagttgtt ggcgaacgag tggcaagagg agcgtctccg tttggcggag
     2461 atgcttcttt ttgctttgtt ctctgcattt tgcttgggtg tgggcatggt gctgctgacc
     2521 atgttcctcg tcgtgctgtt ctgggatgaa caccgtcttg cggtgctggc gagtttgagc
     2581 ctgtttttct ttgtactcgg cgcctggctg attgggttgt tgcggagcag gatgaggcag
     2641 ggatcgaggc tgttttctgt cagtcttgcc gagttgaaca aggatcgagc gtctctgggg
     2701 gcgcgtgatg aatgaccgta tcctcgaact gaggcagcgg cggggtgagt tgcttgcgcg
     2761 cattgctgtg cagcgcggtc agttaatcga gatgtcggcg cgttgcgagg ggccgttggc
     2821 atgggtcgat cagggaatgg ctgtagtgcg cttcctgcgt gccaagccgc tgctggttgc
     2881 cggtgtggcc gcactattgg tcattcgcag gcgcggggtg atggggctgg tgcgcacggg
     2941 atggaagatg tggaagggat accggttctt tacggccatg tcgggaaaac tctcggggag
     3001 agagtagccc gatttccctt agcgcctgcc gccgtaagcg atatgtgtcc gctttgccgt
     3061 tttgattgat gcctgcttcc tgttgtttcc ccgtttggct acgcgctgtc cgcgcgctgg
     3121 cgctttgacc atccggctgg attttccttt tgcagcggtc tgcacgacaa cgagcgtctg
     3181 gccgggtttg atcttaacga tgccatcgtt ccagctcttc agtttggcga gactgacgtg
     3241 atagcgggca gcgatgcggg ccagcgtctc tccttttaat accttgtggc tgatcgaacg
     3301 agcggctggt tcggccgagg gatgcagatg catgttgaat gcggtgaagt cgccatcgga
     3361 ctcattttcc tcgccgttga taggcaccag cagcgtctgt ccggtgctga cgttggatcg
     3421 ccccgacaga ccgttcaccg atttgagttt ttctacggac agtcccagac gcggagccag
     3481 atggtcgagc cgctcgcctt ttttcggctg gtaggcttgc caactgacca gcggcttgtc
     3541 gtaggattcg agattggcgc ggaaggtctc gaccttgtcg atcggcagca ggatcaggtc
     3601 gctgttgtcc tgcaggatca ccggacgatt atgcgccgga ttgagcgcga taaattcttc
     3661 cagcgaaaca tcggccagtt gcgccgccag ctttacgtca atatgttgag cggtggaaac
     3721 ggcggcaaaa tagggttcgt tcggaacatt ctgtagcacg aggccgaagc tggccggatt
     3781 ggcgatgatg ttcttgacgg ccagcaattt tggcacgtag ttgcgcgtct cgtccggcat
     3841 cttcaggctg gtgtaattta ccggcagacc acgtgcgcgg ttgcgcgcct gtgcgcgttg
     3901 caccgcgccc tctccccagt tgtaggcggc cagcgccaat tcccagtcgc cgaacatgtc
     3961 gtgcagtttc tgcaggtagt ccagcgcgcc gttggtcgcg ctgataacgt cgcgtcggcc
     4021 gtcgtaccac cagttttgct ccatgccgaa gtgtttgccg gtagacggaa tgaactgcca
     4081 gatgccggaa gccttgctgg tcgaataggc gccggggttg aatgcactct cgatcatcgg
     4141 taacagggcg atttcgctag gcatgccccg acgctccact tcacccgtga tgtagtagag
     4201 gtagaggcgc gcgcgatcgg tcatgcgggc tacgtaatcc gggcggctgg cgtaccagtg
     4261 ttcgtgtttc gcgatcagcg ggctgtccag ttcgcgcatg gcgaagccgc cgcgtatccg
     4321 cgcccaaagg tcattcatcg ccaggtcgga gtccgggatg agttcaagct tgatgttgcg
     4381 cgcggtcgag agtgtcaggg gaggggtgag ggattcgctg agtgcgagtt gttcgtcggc
     4441 gtaagcgaac ggagtggaaa tgacggcgct agccgcgagc aatgcggaaa aaatcagtcg
     4501 aatcaagggc acgggcattg ctccgggggg tcaaaacatt ggcgatggta gcccagttgc
     4561 cgtcagtggg tcaacctaaa atgtatatac acaaagggtt agctgtgttt tgtgccacat
     4621 tcatgaaatg cgcaggggca cggtgcgccg tgcccctgcg gtcatctttc gaaaatgact
     4681 atttaatcac ttcgttaagc tgctccagga tggcggggtt ttggctctgg tataacgccc
     4741 gctgtcgcgg gcgagccttc gccgacaaca cgcctcgcag aggcttgttg tggtggagac
     4801 taaccttcag gtttgtcgta accaaacccc gccaattaac gaatgacctc ttttaattgt
     4861 tccaagattg ccggattttc cagagtcgag atgtcctgag tgatctcctc gcccttggcg
     4921 agcgtgcgca gcaggcggcg catgatcttg ccggagcggg tcttgggcag gttgtcgccg
     4981 aagcggatct cgtccggctt ggcgatcggg ccgatttcct tgccgaccca gttgcgcagc
     5041 gtgtcggcga gttgcttggc tgctgcggca tccttcgggc gtgcgccttt caataccacg
     5101 aaggcgacga tggcctcacc cttgatgtca tgcggcctgc cgacgatggc ggcctcggcg
     5161 accagtgggt tggcaaccag tgcagattcg atctccatgg tgccgagacg atggccggag
     5221 actttcagca cgtcgtcgtt acgtcccatg atccagacgt agccgtcttc gtcgcggttc
     5281 gcggagtccc ccgcgagata gaagccatgc acttcttccg ggaaatagct cttcttgaaa
     5341 cgctccgggt cgccccagat ggtgcggatc tgcgagggga agggtttctt gatgaccagt
     5401 gagccgccct gctgcatgcc gacttcctcg cccatttcgt ccacgatcgc gatctggatg
     5461 ccgggaatgg gcagggtgca ggaaccgggt ttcagcgctt ccacgccggg cagcggcgcg
     5521 atcatgtggc aaccggtctc ggtctgccac caggtgtcga cgatagggca gcgcgattgt
     5581 ccgatttcgt tgtagtacca catccaggct tccgggttga tcggctcgcc cacgctgccc
     5641 agcaggcgca gcgaggagag gtcgtattgt tttggcaggt cgccgcccag cttgatcagc
     5701 gagcggatcg cggtcggcgc ggtgtagaag gtggtgacct tgtgatcctg gatcatcttc
     5761 cagaagcgtc ccgcgtcggg ataggtcggg atgccttcga acacgacctg ggtcgcgccc
     5821 atcgccagcg ggccgtaggc gacatagctg tggccggtga tccagcccac gtcggcggtg
     5881 caccagaata cgtcgccggc cttgtagtcg aacacccact gcatggtgag catggtgccg
     5941 agcaggtaac ccccgctgga atgttgcacg cccttgggct tgcccgtgga gccggaggta
     6001 tagaggatga acagcggatg ttccgcatcc acccattccg gttcgcaatg cgtgggctgt
     6061 gcattgaaca gctcgtgcca ccagatgtcg cgttcatcgt tccattcgac gctggagccg
     6121 gcgcggcgat aaacgatgac gttccgaaca cagtcgcagc cttcctgtgc cagcgcctcg
     6181 tccacgatgg ttttcagcgg catcaccttg cctccgcgca tctgtgcatc ggcggtgatg
     6241 acggcgcaag cctgggcgtc aacgatgcgc tcatgcaggc tcttggagga gaagccgccg
     6301 aataccaccg aatggatcgc gccgatgcgg gcgcaggcct gcatcgccac gatggcctcg
     6361 atgctcat
//
LOCUS       DASUIV010000134         6988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1241186, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000134 DASUIV010000000
VERSION     DASUIV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241186"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1267
                     /gene="hisC"
                     /locus_tag="VFW59_05040"
     CDS             167..1267
                     /gene="hisC"
                     /locus_tag="VFW59_05040"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5363610.1"
                     /translation="MSSASAASLAQSLVRREILDLHAYHVPDSAGYIKLDAMENPYLV
                     PQALRDEIAGLVSTAAINRYPDPAAASLKAKIRAVSGLPQGMEVMLGNGSDELIQLLA
                     MAVDKPGAVLLSVEPSFVMYKMIATFTGMRYVGVPLTADFELDLAATLDAIEREQPAL
                     VFLAYPNNPTGNLFSADAIKQIIEAAPGLVVVDEAYYAFASDSFIPQLAHYPNLLVMR
                     TFSKLGMAGLRLGFLAGSAAWLEQLEKLRLPYNVGVLPQLVAEKLLDHHEVLLQQAAQ
                     IKQDRAGLYRQLGAVAGVRVYASEANFLLFRVENATAVFDGLKQRGVLIKNLNGGHPM
                     LRDCLRVTVGAHQENERFMAALQDTITQLARG"
     gene            1271..1858
                     /gene="hisB"
                     /locus_tag="VFW59_05045"
     CDS             1271..1858
                     /gene="hisB"
                     /locus_tag="VFW59_05045"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX5363611.1"
                     /translation="MRSAKVSRNTLETQIGVELNLDGTGQAKLDTGLPFLEHMLDQIA
                     RHGLLDINVIAKGDLHIDAHHTVEDIGITLGQAFKQAVGDKKGLCRYGHAYVPLDEAL
                     SRVVLDLSGRPGLVFNCEFVRDNIGEFEADLIHEFFQGFVNHALVTLHIDNLAGNNAH
                     HQAETIFKAFGRALRMALELDPRMAGMMPSTKGSL"
     gene            1863..2495
                     /gene="hisH"
                     /locus_tag="VFW59_05050"
     CDS             1863..2495
                     /gene="hisH"
                     /locus_tag="VFW59_05050"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5363612.1"
                     /translation="MVDVAIVDYGMGNLRSVEQALRRVAPDAEIVVTDDAKVISAAKR
                     VVFPGQGAMPDCMRELDARGLRAAVLEAASSKPFLGICIGLQMLFEHSAEGDVPGLGV
                     LPGEVLRFASDLKDGQGNRLKVPHMGWNRVHHAEHPLWDGIEQDARFYFVHSYYVRPQ
                     DASLAQATSDYPQPFVCAVARGNLFAVQFHPEKSHAAGLKLLQNFVRWIP"
     gene            2513..3298
                     /gene="hisA"
                     /locus_tag="VFW59_05055"
     CDS             2513..3298
                     /gene="hisA"
                     /locus_tag="VFW59_05055"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX5363613.1"
                     /translation="MLIIPAIDLKDGHCVRLKQGMMEDATVFSEDPAATARHWLDQGA
                     RRLHLVDLNGAFAGKPKNEDAVRSIIEEVGDGIPVQLGGGIRDLETIERYLDIGVTYI
                     IIGTAAVKVPGFLHEACDAFPGHIMVGLDAKGGKVAVDGWSKLTGHDVADLAKRFEGY
                     GVEAIIYTDIGRDGMLSGVNIPATVELASPLHVPVIASGGITNLDDVRALCAVQGEGI
                     TGAITGRAIYEGTLDFRAAQALADELTAASGLAIANGWSGAAR"
     gene            3299..4060
                     /gene="hisF"
                     /locus_tag="VFW59_05060"
     CDS             3299..4060
                     /gene="hisF"
                     /locus_tag="VFW59_05060"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX5363614.1"
                     /translation="MMLAKRIIPCLDVTAGRVVKGVSFVELRDAGDPVEIARRYDEQG
                     ADELTFLDITASSDDRGIIFRIIEQVASQVFIPLTVGGGVREVGDVRNLLNAGADKVS
                     INTSAVVSPQLVADAAARYGSQCIVVAIDAKQVAPGKWEVFTHGGRKATGLDAIEWAK
                     KMQTLGAGEILLTSMDRDGQKNGFDLKLTRAVTDALEIPVIASGGVGNLQHLADGVKL
                     GGADAVLAASIFHFGEYTVQQAKQYMNEQGIEVRL"
     gene            4057..4452
                     /gene="hisI"
                     /locus_tag="VFW59_05065"
     CDS             4057..4452
                     /gene="hisI"
                     /locus_tag="VFW59_05065"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX5363615.1"
                     /translation="MSDTWLNKVNWSDDGLVPAIAQDAASGRVLMVAWMNREALKQTW
                     QKGEAVYWSRSRKKLWHKGEESGHVQKVKEIRLDCDSDVVLLQIEQQGGIACHTGRES
                     CFYSRLEQGQWVAVDEVLKSPDEIYGKKS"
     gene            4453..4770
                     /locus_tag="VFW59_05070"
     CDS             4453..4770
                     /locus_tag="VFW59_05070"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX5363616.1"
                     /translation="MNEDILQRLTETLEARKQASPDSSYVAKLFGKGEDAILKKVIEE
                     AGEVLLAAKDGDAAHLVYEVADLWFHTLVLLAQKGLSADDVLRELARREGLSGIAEKA
                     SRK"
     gene            4767..5114
                     /locus_tag="VFW59_05075"
     CDS             4767..5114
                     /locus_tag="VFW59_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX5363617.1"
                     /translation="MSDCLFCKIARGEIPSRKLYEDDEVLAFHDINPVAPVHFLLIPK
                     RHLESLLDVEEQHAPLLGRMLVLAAKLAKEQGLEKGFRTVINNGKGGGQEVFHLHIHA
                     IGGGSIPPMVKRN"
     gene            5129..5347
                     /gene="tatA"
                     /locus_tag="VFW59_05080"
     CDS             5129..5347
                     /gene="tatA"
                     /locus_tag="VFW59_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEX5363618.1"
                     /translation="MGSMSIWHWLIVLVVVMLVFGTKKLRNIGSDLGGAVKGFKEGMR
                     SDEDAPKQLDEGGKTIEGEVKDKKTTKA"
     gene            5370..5750
                     /gene="tatB"
                     /locus_tag="VFW59_05085"
     CDS             5370..5750
                     /gene="tatB"
                     /locus_tag="VFW59_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX5363619.1"
                     /translation="MFDFSLPELMVVLVVALIVIGPERLPKVARTLGHLYGRAQRYVN
                     GVKADIARDMAIEEFRQLQQKVQEEASAAERSVRLVEQALDQQVQQINRAVAQAPAQQ
                     AIQPAIEPPQAASVEASTVTSTKS"
     gene            5750..6502
                     /gene="tatC"
                     /locus_tag="VFW59_05090"
     CDS             5750..6502
                     /gene="tatC"
                     /locus_tag="VFW59_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5363620.1"
                     /translation="MSTSETLIAHLLELRTRLLNSVLALVSVFICLFPWASDLYALLA
                     QPLLEKLPKGGQMIATDVTTPFFVPLKVAMMAAFLIALPYILYQIWRFVAPGLYAHEK
                     RLVVPLIVASTLLFFCGMAFAYFVVFPVVFGFITASAPHGVAVMTDIDKYLSFVLSMF
                     VAFGITFQVPVVVVVLVRMGFVTVEKLREIRPYIVVGAFVVGAIFTPPDVVSQFMLAM
                     PLWLLYEAGIVVASWIKPADSLADQKNNGINT"
     gene            6623..>6988
                     /locus_tag="VFW59_05095"
     CDS             6623..>6988
                     /locus_tag="VFW59_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX5363621.1"
                     /translation="MPAFINTNIASLNAQRNLNQSQSALQTSLQRLSSGLRINSAKDD
                     AAGLAISDRMTAQIRGLDQARRNANDGVSMAQTGEGALAGASDMLQRIRELAVQSANA
                     TNSASDRAALQSEAGLLVSE"
BASE COUNT         1348 a         1920 c         2300 g         1420 t
ORIGIN      
        1 cgatgaaggc agcgctctgg agttgattgc agagttgaag ctgcgcaaac aacaggcacc
       61 tgccgaaaat atttagcaga cggacgtgcc cctcattcgg actgcgtctg gtttgtgata
      121 agcttcgtgc cactgtaagc ccactcgttt attgaacgat tcattcatgt cttccgcttc
      181 tgctgcttcc ctcgcccagt ctctggtgcg ccgcgagatt ctcgatttgc acgcctacca
      241 cgtgccggac agtgcgggct atatcaagct cgatgcgatg gagaacccct atctggtgcc
      301 gcaagccttg cgcgatgaga tcgccgggct ggtctcaact gccgccatca atcgttatcc
      361 cgatcccgcc gccgcctcgc tcaaggccaa gatacgggcc gtgtctggcc tgccgcaagg
      421 catggaggtg atgctgggca acggttcgga cgagctgatc cagttgctgg cgatggcggt
      481 cgacaagccc ggtgcggtgt tgttgagcgt ggagccgtcg tttgtgatgt acaagatgat
      541 cgcgacgttt accggtatgc gttatgtcgg cgtgccgctg acggcggatt tcgagctgga
      601 tctggccgcg acgctggatg cgatcgagcg cgaacagccc gcgctggtgt ttctggccta
      661 cccgaacaac cctaccggca atctgttttc tgccgatgcg atcaagcaga tcatcgaggc
      721 agcgcccggt ctggtggtgg tggacgaggc ctattatgcg ttcgccagcg acagcttcat
      781 tccgcagttg gcgcattacc cgaatctgct ggtgatgcgc actttctcca agcttggcat
      841 ggcgggcttg cgcctcggct ttctggcggg gagcgcggca tggctggagc aactggagaa
      901 attgcgcttg ccgtataatg tcggcgttct gccgcaactg gtggcggaga agttgctgga
      961 ccatcacgag gtattgctgc agcaggcggc gcagatcaag caggaccgtg cgggactgta
     1021 ccggcagctg ggcgcagtcg ccggagtgcg ggtctatgcc agcgaggcga acttcctgct
     1081 gtttcgcgtg gagaacgcga cggcggtgtt tgacggactg aagcagcgcg gcgtattgat
     1141 caagaatctg aacggcggac atcccatgtt gcgggattgt ctgcgcgtga ctgtcggggc
     1201 gcatcaggag aacgagcggt tcatggcggc actgcaagac actattactc aactcgcacg
     1261 gggctaagtc atgcgtagcg cgaaagtttc acgcaatact ctggagacgc agatcggcgt
     1321 cgaattgaat ctggacggca ccgggcaggc caagctggat accgggctgc cgttcctcga
     1381 gcacatgctc gatcagatcg cgcgacacgg tctgctggat atcaacgtta tcgccaaggg
     1441 cgatctgcat atcgatgcgc accacaccgt cgaagacatc ggcattacgc tggggcaggc
     1501 gttcaagcag gcagtcggcg acaagaaggg tttgtgccgt tacggccatg cctatgtgcc
     1561 gctggacgaa gccctgtcgc gcgtagtgct ggatctctcg gggcgtcccg ggctggtgtt
     1621 caactgcgaa ttcgtgcggg acaacatcgg tgaattcgag gcggatctga tccatgaatt
     1681 cttccagggt ttcgtcaatc acgcgctggt gacgctgcac atcgataatc tggctggcaa
     1741 caatgctcac catcaggccg agacgatatt caaggcattc ggtcgcgcgc tgcgcatggc
     1801 gctggagctc gatccgcgca tggcgggcat gatgccttcc accaaagggt cgctataact
     1861 ccatggtcga cgtcgcgatt gtagattacg ggatgggcaa tttgcgctcg gtcgaacagg
     1921 ccttgcgccg tgttgctccc gatgccgaaa tagtggtcac cgacgatgca aaggtgatct
     1981 ccgccgcaaa gcgcgtggtc ttcccggggc agggtgcgat gccggactgc atgcgcgagc
     2041 tggatgcgcg cggcttgcgc gccgccgtgc tggaagcggc gagcagcaag cctttcctcg
     2101 gtatctgcat cggtctgcag atgttgttcg aacacagtgc cgagggcgat gtgccggggt
     2161 tgggcgtgtt gccgggcgag gtattgcgct tcgcctccga tctcaaggat ggacagggca
     2221 accgattgaa agtgccgcac atgggctgga atcgggtgca tcatgccgaa catccgttgt
     2281 gggatggcat cgagcaggat gcgcgtttct atttcgtgca cagttactat gtgcgtccgc
     2341 aggatgcctc gctggcacag gctaccagcg attatcctca accgttcgtg tgtgccgtgg
     2401 cgcggggcaa cctgttcgcg gtgcagtttc acccggaaaa aagccatgcg gcagggctta
     2461 agctgctgca aaattttgtg cgatggatcc cttaaactct taacgtattg ccatgctgat
     2521 tattcctgcg attgatttga aagacggtca ttgtgtgcgc ctgaaacagg gcatgatgga
     2581 ggatgccacc gtcttctccg aagaccctgc ggctaccgcg cgtcactggc tggatcaggg
     2641 cgcgcgtcgc ctgcatctgg tggacttgaa cggtgccttc gccggtaagc ccaagaatga
     2701 agatgcggtg cgcagcatca tcgaggaggt gggcgatggc atcccggtac aactgggcgg
     2761 cggtatccgt gatctggaaa ccatcgagcg ttatctcgat atcggcgtga cttacatcat
     2821 catcggcacg gcggcggtga aggtgcccgg tttcctgcat gaagcctgcg acgcgttccc
     2881 tggccatatc atggtcggtc tggatgccaa gggtggcaag gtggcggtgg acggctggtc
     2941 caaactaacc ggtcacgatg tggccgacct ggcgaagcgc ttcgagggtt acggcgtcga
     3001 agcgatcatt tacaccgaca tcggccgcga cggcatgctg tccggtgtga acatcccggc
     3061 gaccgtggag ctggccagcc cgctgcatgt gccggtgatc gccagcggcg gcatcaccaa
     3121 tctggacgat gtgcgcgcat tgtgcgcggt gcagggcgaa ggcatcaccg gggcgatcac
     3181 cggtcgtgcg atctacgaag gcacgctgga cttccgggca gcgcaggcgc tggcggacga
     3241 gctgactgca gcgagcggac tggccattgc gaacggatgg agcggcgcgg cgcgctagat
     3301 tatgctggct aaacgcatca ttccctgtct ggatgtgacc gccggccgcg tggtcaaggg
     3361 ggtcagcttc gtcgagttgc gcgatgcggg cgaccctgtc gaaatcgcgc gccgctacga
     3421 cgaacagggg gcggacgaac tgacctttct cgacatcacc gccagctcgg acgaccgtgg
     3481 catcatcttc cgcatcatcg aacaggtggc gtcgcaggtg ttcattccgc tgacggtggg
     3541 cggcggcgtg cgcgaggttg gcgacgtgcg caatctgctg aatgcggggg ccgacaaggt
     3601 cagcatcaat acttctgcag tggtgagccc tcagctggtg gccgacgccg cagcgcgtta
     3661 cggttcgcag tgcatcgtgg tggccatcga cgccaagcag gttgcacccg gcaagtggga
     3721 ggtgttcact cacggcggcc gcaaggctac cggcctggat gccatcgaat gggccaagaa
     3781 aatgcagacg ctgggcgcgg gagaaattct gttgaccagc atggatcgcg acggtcagaa
     3841 gaacggtttc gatctgaaac tcacccgtgc ggtgaccgat gcgctggaga ttccggtgat
     3901 cgccagcggc ggcgtgggca acttgcagca tctggccgac ggcgtgaagc tgggcggcgc
     3961 cgatgcggtg ctggcggcca gcatcttcca tttcggcgaa tacaccgtgc agcaggccaa
     4021 gcagtacatg aacgagcagg gcatcgaggt gcgcttgtga gcgatacctg gctgaacaag
     4081 gtgaactggt ccgatgacgg gctggtgccc gctatcgcgc aggacgccgc cagcgggcgg
     4141 gtgctgatgg tggcgtggat gaaccgcgag gccctgaagc agacctggca aaaaggcgag
     4201 gcggtgtact ggtcgcgttc gcgcaagaag ctgtggcaca agggcgagga atccggccat
     4261 gtgcagaagg tgaaggaaat tcgccttgac tgcgacagcg acgtggtgct gctgcagatc
     4321 gaacaacagg gcggtatcgc ctgccatacc gggcgcgaga gctgtttcta cagccgtctg
     4381 gagcaggggc agtgggtggc ggtggacgag gtgctgaaat cgcccgatga gatttatggg
     4441 aagaaatcct gaatgaacga agatattctg caacgcctga ccgagacgct ggaggcgcgc
     4501 aaacaggcgt cgcccgacag ttcctatgtc gccaagttgt tcggcaaggg tgaggatgcg
     4561 atcctgaaaa aagtgatcga ggaggcgggc gaggtgttgc tggccgccaa ggatggcgac
     4621 gcagcgcatc tggtctacga agtggccgat ctgtggtttc acactctggt gttgctggcg
     4681 cagaagggct tgagtgcgga cgatgtgctc agagagctgg cgcggcgcga aggcctgtcg
     4741 ggtatcgccg agaaggcgag ccggaaatga gcgattgcct gttctgcaag attgcacgcg
     4801 gcgagattcc cagccgcaag ctgtacgagg atgacgaggt actggcgttc cacgacatca
     4861 accccgtcgc gccggtgcat tttctgttga ttccgaagcg gcaccttgag tcgctgctgg
     4921 atgtggagga gcagcatgcc ccgttgctgg gcaggatgct ggtgctggct gccaagctgg
     4981 ctaaggaaca aggattggag aagggcttcc gcaccgtgat caataacggc aagggcggcg
     5041 gtcaggaagt gtttcatttg catattcacg cgatcggcgg tggcagcatc ccgcccatgg
     5101 tcaaacgcaa ttaactgagg agagaaaaat gggttcgatg agtatttggc attggctgat
     5161 cgtgttggtc gtggtgatgt tggtgttcgg caccaagaag ctgcgcaaca tcggcagcga
     5221 tctgggcggt gcggtgaagg gcttcaagga aggcatgcgc tccgatgagg acgcgcccaa
     5281 gcagttggat gagggcggga agaccatcga aggcgaagtg aaagacaaga agaccaccaa
     5341 ggcctgagcc gtgtcgagag ctgccgaaca tgtttgactt cagcctcccc gaattgatgg
     5401 tcgtgctggt ggtggcgctg atcgtcatcg ggcccgagcg tctgcccaag gtggcgcgta
     5461 cgctggggca tctgtacggt cgagcgcagc gttatgtgaa tggcgtgaag gcggatatcg
     5521 cacgcgacat ggcgatcgaa gagttccgtc aattgcagca gaaggtgcag gaagaggcta
     5581 gcgcggccga gcgttcggtg cggttggtcg aacaggcgct ggatcagcag gtgcagcaaa
     5641 tcaatcgtgc ggtagcgcag gcccccgcgc agcaggcaat acagcccgct atcgagccgc
     5701 cgcaggccgc gtcggtcgaa gcatctacgg ttacatccac taaatcctga tgagcaccag
     5761 cgaaaccctg atcgcccacc tgctcgaact gcgcacccgt ttactgaatt ccgtgctggc
     5821 tctggtgtcg gtgttcatct gtctgttccc ctgggcgtcc gatctctatg cgctgctggc
     5881 gcaaccgttg ctggaaaagt tgcccaaggg cgggcagatg atcgccaccg atgtcaccac
     5941 gccgttcttc gtgccgctca aggttgcgat gatggcggcc ttcctgatcg cgctgcctta
     6001 catcctgtac cagatatggc gcttcgttgc ccccggcctg tacgcgcatg agaagcgtct
     6061 ggtcgtgccg ctgatcgttg ccagtaccct gctgttcttc tgcggcatgg cgtttgccta
     6121 tttcgtggtg ttcccggtgg tgttcggttt catcacggcc tcggctccgc acggcgtggc
     6181 ggtgatgacc gatatcgaca aatatctcag cttcgtgctg tccatgttcg tggcgttcgg
     6241 catcaccttt caggtgccgg tggtcgtggt agtgctggtg cgcatgggct tcgtcacggt
     6301 agagaagctg cgcgagatac gcccctacat cgtcgtcggt gccttcgtgg tcggcgcgat
     6361 cttcaccccg cccgatgtgg tgtcgcaatt catgctggcg atgccgctgt ggctgctgta
     6421 cgaggcgggc atcgtggtgg cgagctggat caagcctgcc gactcgctgg cagatcaaaa
     6481 aaataatggc ataaatacct aaggtttttc gctcggcttc cgataaccga tctaatggca
     6541 cacacagtgc gactcataaa ctggccacgg caactcgtgt ctcactttaa gagtgatcga
     6601 aatctaaggg agccctgtcg aaatgccggc atttatcaat accaacattg cgtcgctgaa
     6661 tgcgcagcgc aacctgaacc agtcccagag cgccctgcag acctccttgc agcgtctgtc
     6721 ttccggcttg cgtatcaaca gcgccaagga cgatgcggct ggcctggcga tctccgaccg
     6781 gatgaccgca cagattcgcg gcctcgatca ggctcgccgc aacgccaacg acggcgtttc
     6841 gatggctcag accggcgaag gcgcgttggc cggtgcttcc gacatgctgc agcgtatccg
     6901 cgaactggcc gtgcaatccg ccaacgccac caacagcgca agtgaccgtg ccgcactgca
     6961 atccgaggca ggcctgctgg tctccgaa
//
LOCUS       DASUIV010000135         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_960868, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000135 DASUIV010000000
VERSION     DASUIV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960868"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1491
                     /locus_tag="VFW59_05100"
     CDS             73..1491
                     /locus_tag="VFW59_05100"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5363622.1"
                     /translation="MRLLIVTLALSLLAGCAQNPVTGQTDFVMMTEKQEMAVGRQSDE
                     QIKKQYKVYESKPLQDYVNSVGQRIAKQSHRPNLQYHFTVLDSTEVNAFALPGGYVYI
                     TRGIMAYLNSEAELAAVLGHEIGHVTARHGVRQQSAAQAANIGLTIASIFVPELGSNV
                     GQQLGNVVGGALLSGYGRDHELEADHLGAQYLARSDYDPQAIIAILRTLKNQELKDAE
                     LAKQEGREPRRYSGLFATHPDNDTRLHQVVGEADKLAPAVPFEGREEFLRNIDGLVYS
                     DDSEQGVVRNNRFYHGDMGFALQFPETWMVHNLPNALVAISPQGEAQMQLKLDAKPVG
                     TPDEYARRKVGGSARIESFDVNGLPAALAELPGSIAGVIYLDKQAFVVQAQAKTKEAL
                     AARRDEIVGTIRSFHKLTAEERKLVKPLAIRIITVKAGDTYAALAKHSPLGKSAESYL
                     RLMNANYPEGEPQPGQRIKIVE"
     gene            1496..3145
                     /locus_tag="VFW59_05105"
     CDS             1496..3145
                     /locus_tag="VFW59_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5363623.1"
                     /translation="MYFIKPTDPYPITPAKAAMLALLCLIWLGTGLVGHDPWKPDEAY
                     SFGLVYSMLQSGDWPNAWLVPTLAGEPYMDKPPLFYWTAALFAKLCAPLLPLHDGARL
                     ASGFYSALTMLFIGLTGRKLYGENRGWAAAVILIGCIGMLVRAHQMITDLALLAGCAM
                     MLYGFAQTQERVLRPGFWIGMGMGISFMAKGFIAPVLFALIAALLPALFKNWRLRSYY
                     SSMGIAALCALPWLSVWPLLLYLHSPQLFADWAWSHNIGNWFDYIKAGPNRDSFYYLK
                     NLPWLAWPALPLAAWAVWQSRKRLAQRDDLQLPLVAFAVMLVTLSLVPNIKEVFALPM
                     LLPIALLATASLSMLKRGAANALDWFGLMTFALLAIAMWWGWAGLLLDNHAKITFWLK
                     DYQPGFDPDFQTAPFVIAIIASALWLVLVWRVGRSMRRAVVNWAAGITLLWILAMTLW
                     LPWLDSGKSYRSMVASLKQALPEQYQCIKGKHLGDGQRAMLHYFGNIITRHDHEHRCD
                     LLLIQGERNSRRLSEGQWKMIWEGSRKGDKGEHYRLYRKVG"
     gene            complement(3237..3719)
                     /locus_tag="VFW59_05110"
     CDS             complement(3237..3719)
                     /locus_tag="VFW59_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363624.1"
                     /translation="MIETATIITLCLLYLIFFRPGKTPPLENPLVIERAGQYHMTLAP
                     QLNLAQPFVEEIARQVEVSSAASSASQFFAVCDKQVTAHGYDFYLLAVTQRDGMLFFQ
                     AARPVSREPESELHTIGEFADKVLARFPDGAHDVALDDGLRLAVQRAAQLRGVELRRL
                     "
     gene            complement(3759..4139)
                     /locus_tag="VFW59_05115"
     CDS             complement(3759..4139)
                     /locus_tag="VFW59_05115"
                     /inference="COORDINATES: protein motif:HMM:NF020480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity protein Tdi1 domain-containing
                     protein"
                     /protein_id="HEX5363625.1"
                     /translation="MPVLFTALGDVFFQPDSGGIWWLNTGTAEVTRVAGSVDQFRELL
                     GTEIVDEWFLPSLIEQLHAAGKVPDEGECYTYVTLPVFEEGKYEVENLNPVPAYEHFA
                     LTGDIHCEILSRPLGGKSEPDVAS"
     gene            complement(4297..>5010)
                     /locus_tag="VFW59_05120"
     CDS             complement(4297..>5010)
                     /locus_tag="VFW59_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5363626.1"
                     /translation="CALAYHERRAILDGNVKRVLARWCGIEGWAGDKKVEEKLWQQAE
                     ALLPHSSPRPAGERVRERGGSDSAIAIYIQAQMDMGATVCTRSKPKCGECPVQADCIA
                     FQAQRVSELPTPRPRKAVPERHTTFLLLMSGRDILLEKRPSSGIWGGLWCPPQLDDGQ
                     GIAEDYVQRNGVEVSERSELAEFTHVFTHFKLHITPVLLRLARKPQRVQQPGSVWLDV
                     EEALRAAIPTPVRKVLEQL"
BASE COUNT          989 a         1644 c         1477 g          900 t
ORIGIN      
        1 aaaggcagcg ttcgctgcct tttttgtttt tgccgcgaca atatttagac tctcaattga
       61 actggaattg ccatgcgtct tctgatcgtt actcttgccc tctcactgct tgccggttgc
      121 gcgcaaaacc ccgtcaccgg acaaaccgac ttcgtgatga tgaccgaaaa acaggagatg
      181 gccgtcggtc gccagtccga tgaacagatc aagaagcaat acaaagtcta cgaatcgaaa
      241 ccgctgcaag actatgtgaa cagcgtcggc cagcgcatcg ccaagcaaag ccaccgcccc
      301 aacctgcaat accacttcac cgtgctggac tcgaccgaag tcaacgcctt cgcgctgccc
      361 ggcggctatg tgtatatcac gcgcggcatc atggcctatc tcaacagcga agcggaactg
      421 gccgccgtgc tgggccacga aatcggccac gtgacggcgc gccacggcgt gcgccagcaa
      481 agcgccgcac aggcggccaa catcgggctg accatcgcat ccattttcgt gccggaactc
      541 ggctcgaacg tcggccagca actcggcaac gtcgtcggcg gcgcgctgct gtccggttac
      601 ggacgcgacc acgagctgga ggccgaccac ctcggcgcgc agtatctggc gcgctccgac
      661 tacgatccgc aggccatcat cgccatcctg cgcacgctga aaaatcagga gctgaaggat
      721 gccgaactgg ccaagcagga aggccgcgag ccgcgccgct actccggcct gttcgccacg
      781 cacccggaca acgacacgcg cctgcatcag gtggtgggcg aagcggacaa actggcgccc
      841 gccgtgccgt tcgaagggcg cgaggaattc ctgcgcaaca tcgacggtct ggtgtacagc
      901 gacgacagcg agcagggcgt cgtgcgcaat aaccgcttct accacggcga tatgggcttc
      961 gcgctccagt tcccggaaac ctggatggtg cataacctgc cgaacgcgct ggtcgcgatc
     1021 agcccgcagg gcgaagcgca aatgcaactc aagctggacg caaaacccgt cggcacgccc
     1081 gacgaatacg cgcgccgcaa agtgggcggc agcgcgcgca tcgaatcgtt cgacgtgaac
     1141 ggcctgcccg ccgcgctggc cgagttgccc ggcagcatcg ccggggtgat ctatctggac
     1201 aagcaggcgt tcgtcgtgca ggcccaggcc aaaaccaagg aagcgctggc ggcgcggcgc
     1261 gacgaaatcg tcggcacgat acgcagcttc cacaagctca ccgccgaaga acgcaaactg
     1321 gtcaagccgc tcgcaatccg catcatcacc gtcaaggccg gcgacaccta tgccgcgctg
     1381 gcgaagcatt ccccgctcgg caaatcggca gaaagctatc tgcgcctgat gaatgccaac
     1441 tatcctgaag gcgaaccgca gcccggacag cgcatcaaga tcgtggaata agccgttgta
     1501 cttcataaaa ccgaccgacc cctatcccat cacccccgcc aaggcggcga tgctggcgct
     1561 gctgtgcctg atctggctcg gcacggggct ggtcggccac gacccgtgga agccggacga
     1621 ggcgtacagt ttcgggctgg tatattcgat gctgcaaagc ggcgactggc ccaatgcgtg
     1681 gctggtcccc accctcgcgg gcgagcccta tatggacaag ccgccgctgt tctactggac
     1741 ggccgcgctg ttcgccaagc tgtgcgcgcc gctgttgccg ctgcacgacg gcgcgcgcct
     1801 cgccagcggt ttttacagcg cgctgaccat gctgttcatc ggcctgaccg gacgcaaact
     1861 gtacggcgaa aatcgcggct gggcggcggc ggtcatcctg atcggctgca tcggcatgct
     1921 ggtgcgcgcg caccagatga ttaccgatct ggcgctgctc gccggttgcg caatgatgct
     1981 gtacggcttc gcgcaaacgc aggaacgcgt gctgcgcccc ggcttctgga tcggcatggg
     2041 catgggcatc agcttcatgg ccaaaggctt catcgccccg gtgctgttcg cgctgatcgc
     2101 cgcgctgctg cccgcgctgt tcaagaactg gcggctgcgc agctactatt ccagcatggg
     2161 catcgcggcg ctgtgcgcgc tgccctggct atcggtatgg ccgctgctgc tctacctgca
     2221 ctcgccgcaa ctgttcgccg actgggcgtg gtcgcacaac atcggcaact ggttcgacta
     2281 catcaaagcc gggccgaacc gggactcctt ctactacctg aaaaacctgc cctggctggc
     2341 ctggcccgcg ctgccgctgg cggcctgggc ggtgtggcaa tcgcgcaaac ggctcgcgca
     2401 acgcgacgac ctgcaactgc cgctggtcgc cttcgcggtg atgctggtca cgctcagcct
     2461 ggtgcccaac atcaaggagg tgttcgcgct gccgatgctg ctgccgatcg cgctgctggc
     2521 gaccgcctcg ctgtcgatgc tgaaacgcgg cgcggccaac gcgctggact ggttcggcct
     2581 gatgaccttc gcgctgctcg ccatcgcgat gtggtggggc tgggccggcc tgctgctgga
     2641 caaccacgcc aaaatcacgt tttggctgaa agactaccag cccggcttcg acccggattt
     2701 ccagactgcg ccgttcgtca tcgcgatcat cgccagcgcg ctgtggctgg tgctggtgtg
     2761 gcgcgtcggg cgctcgatgc gccgcgcggt ggtgaactgg gccgcaggca tcacgctgct
     2821 ctggatactc gcgatgacgc tgtggctgcc ctggctggac agcggcaaga gctaccgcag
     2881 catggtcgcc tctctgaagc aagcgctgcc ggagcaatac cagtgcatca agggcaagca
     2941 tcttggcgac gggcagcgcg cgatgctgca ttacttcggc aacatcatca cccggcacga
     3001 ccacgaacac cgctgcgacc tgctgctgat ccagggcgag cgcaactcgc gcaggctaag
     3061 cgaaggacag tggaagatga tatgggaagg cagccgcaag ggcgacaagg gcgagcatta
     3121 ccggctgtac cggaaagttg gataaggccc gtaggctggg ttgagcgtgt ttcgatggtg
     3181 atgggtatcg ctacgctcaa cccatcctac cctgctgtcg ctcaacccag cctacgtcac
     3241 aaccgcctga gctctacacc gcgcaactgc gccgcacgct gcaccgccaa gcgcaacccg
     3301 tcatccagcg caacatcgtg cgccccatcg gggaagcgcg ccagaacctt gtcggcgaat
     3361 tcgccgatgg tatgcaactc gctctcgggc tctctggaga ctggccgggc cgcctgaaaa
     3421 aacaacatcc cgtcgcgttg cgtcaccgcg agcaaataga aatcatagcc atgcgcggtc
     3481 acctgcttgt cgcacaccgc gaagaattgc gacgcgctgc tggcggcgct cgacacttcg
     3541 acctgccttg cgatctcttc cacaaacggc tgcgccagat tgagctgcgg cgcaagcgtc
     3601 atgtgatatt gcccggcccg ctcgatcacc agcggattct ccagcggcgg cgtcttgccg
     3661 ggacggaaga aaatcagata gagcaggcac agcgtgatga tggtggcggt ttcgatcatg
     3721 agcgttttcc tgtttgacct tgccgttaat gaatcgagtt aactcgcaac atcaggttcg
     3781 ctctttccac ccaacggacg cgagaggatt tcgcaatgaa tatcgcccgt cagcgcgaaa
     3841 tgctcataag ccggaacggg attgaggttc tcgacctcgt actttccttc ttcgaagaca
     3901 ggaagcgtga cgtaggtata acactcgcct tcatcaggca cttttcctgc ggcatgcaac
     3961 tgttcgatca gactgggcag gaaccattca tcgacgatct cggtacccag caactcccgg
     4021 aactgatcga cagaaccggc cactcgcgtc acctctgccg tacccgtatt gagccaccag
     4081 ataccgcccg agtcgggttg aaaaaacaca tcgccgagcg cggtaaaaag cacgggcaca
     4141 aaaggctcgc gtatcaacca gccccaggaa acgccgagct gttgaaccga ttcctcggag
     4201 ggagtgcaag taagtgaaga ccatcctata gacatgaagt atctacctta aattttgtaa
     4261 atgaaaacca tcgggggtag cccgacggtg ttggttttag agttgctcca aaaccttccg
     4321 caccggcgtc ggtatcgccg ccctcaacgc ctcttccaca tccagccaaa cactccccgg
     4381 ctgttgcacc cgctgcggct tgcgtgccag ccgcagcagc actggcgtga tgtgcagttt
     4441 gaagtgcgtg aagacatgcg tgaactcggc cagttccgac ctctcgctca cctccacgcc
     4501 gttgcgctgc acgtaatcct cagctatccc ctgcccgtca tccagttgcg gcggacacca
     4561 cagcccgccc cagatgccgc tgctggggcg cttctccagc aggatgtcgc ggccagacat
     4621 cagcagcagg aaagtggtgt gccgttccgg cacggccttg cgcgggcgcg gcgtggggag
     4681 ttcggacacg cgctgcgctt gaaaagctat gcagtcggcc tgcaccggac actctccgca
     4741 cttcggtttg ctgcgcgtgc agaccgtcgc gcccatgtcc atttgcgcct ggatgtagat
     4801 ggcgatggca ctatcagaac cgcccctctc cctaaccctc tcccctgcgg ggcgagggga
     4861 tgaatgaggc agcagcgcct cggcctgctg ccacaacttt tcttccacct tcttatcccc
     4921 ggcccaaccc tcgatgccgc accagcgcgc cagcacgcgc ttgacgttgc cgtccaggat
     4981 cgcgcgtcgc tcgtggtaag ccagcgcgca
//
LOCUS       DASUIV010000136         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1148599, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000136 DASUIV010000000
VERSION     DASUIV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148599"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1090)
                     /locus_tag="VFW59_05125"
     CDS             complement(86..1090)
                     /locus_tag="VFW59_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HEX5363627.1"
                     /translation="MSAVKNAIENIQGYFAHPLMLLALFVVASFVMIWRLHAMESKGF
                     EGTVLGTLIMPYCSGFANLIFAYVMSSSASNGGLVIENALVNNATNLTLLLGLSAIFG
                     SAVVMQKGGSKQKAKQHAEFHRINRLNLLFTLIALFLFTGTLWALAKDGVLDFYDGLV
                     LVGLFLFWQVLHVFEVLKDNIRKNKGFHWTILIDVALLAASAYCIYYAVDHLVAWVST
                     TESPYFSFAKLGWFSGLLMVVPNAFLALYYTRINRQDIVLSSQIGDGHICIPMCIGLF
                     ALFNPIQIPAFFQMGTYIILGAGAVHFLSIAFIGRIPKFVGFGLIGGYGVFLYQGLIG
                     "
     gene            1342..3204
                     /gene="mutL"
                     /locus_tag="VFW59_05130"
     CDS             1342..3204
                     /gene="mutL"
                     /locus_tag="VFW59_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX5363628.1"
                     /translation="MPSIHLLPDLLINQIAAGEVIERPASALKELMENSLDAGASDIA
                     VQLEGGGIKLLRIRDNGRGIAKEQLALALMRHATSKIASLEDLQKVASMGFRGEALAS
                     MAAVAQLTLTSRTTTPSRTAEDTHGWKIEAIDGRLSEPAPTSQPQGTTVELRELYFNT
                     PARRKFLKSEATEFAWCDEAFKRIALSRPDVAFSLQHNGKTIRQLPVQSIAARIAAIL
                     GAEFGQAAVVVERQAANLSMQGLAALPAYSRSSRDAQYFFVNGRFVRDKVASHALRQA
                     YQDILHHQRHPAFVLFLDMPPEQVDVNVHPAKSEVRFRESQGIHQFIFHTLQESLSVT
                     MGECEAPAQTVAPTNTYVAPQFRQQAMTLGVAEREATYRLWETQTGSSQSEVVSGEWR
                     ERSEGQGSGKTTAAPYSPLTTDHLPSPTDHSPANTHPLGYAIGQLSGVYILAQNTQGM
                     IVVDMHAAHERIVYERLKAALDAERIASQPLLIPVSFYAEPLDVATVEAEQEALHKLG
                     FDLAPLSPTQLALRAMPTMLKQSEAETMALEVLRELREYGASRALTERRNELLASLAC
                     HAAVRANHALSLTEMNALLREMEQTERADQCNHGRPTWFQMTMGDLDKMFMRGK"
     gene            3309..>3857
                     /locus_tag="VFW59_05135"
     CDS             3309..>3857
                     /locus_tag="VFW59_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5363629.1"
                     /translation="MTASPSIFRIAFASFIGTAIEFYDFYIYGLAVAMVIGPVFFPND
                     DPAVQALNAFLTFGIAFLARPIGALVFGHFGDRVGRKATLVASLLVMGISTTLIGVLP
                     SYEMVGWTAPLLLCLLRFGQGIGLGGEWGGAALLAAEHAPKGRRGWFGMFPQLGPPVG
                     FIFAVGGFLLLSSLLTDEQFRSW"
BASE COUNT          790 a         1253 c         1160 g          654 t
ORIGIN      
        1 gtagggtgca ataagcgcag cgcattgcac cggatttccc gccagcaacc ggtgcaatgc
       61 ccgttggtta ttgcacccta cacaatcaac cgatcaaccc ctgatacagg aacaccccat
      121 agcccccgat cagcccgaag ccgacaaatt tcgggatgcg tccgatgaag gcgatcgaca
      181 ggaaatgcac cgcgcccgcg ccgaggatga tgtaagtgcc catctggaag aacgcaggaa
      241 tctggatcgg gttgaacagc gcgaacaggc cgatgcacat cgggatgcag atatgcccgt
      301 cgccgatctg cgagctcagc acgatgtcct gccggttgat gcgcgtgtaa tacagcgcga
      361 ggaaggcgtt cggcaccacc atcagcaagc cgctgaacca gcccagtttg gcgaaactga
      421 aatacgggct ttcggtggtc gagacccatg ccacgagatg gtcgaccgcg taatagatgc
      481 aataggcgct ggccgcgagc agcgcgacgt cgatcaggat agtccagtgg aatcccttgt
      541 tcttccggat gttgtccttc agcacctcga acacgtgcag cacctgccag aacaggaaca
      601 gcccgaccag caccagaccg tcgtagaagt ccagcacgcc gtccttggcc agcgcccaga
      661 gcgtgccggt gaacaggaac aaggcgatca gcgtgaacag cagattgagc cggttgatgc
      721 ggtggaattc cgcgtgctgt tttgctttct gttttgagcc gcccttttgc atgacgacag
      781 ccgaaccgaa gatcgccgac aggccgagca gcagcgtcag gttggtcgcg ttgttgacga
      841 gcgcgttctc gatgaccagt ccgccattgc tggccgagct gctcatcaca taggcgaaga
      901 tcaggttcgc gaagccggag cagtaaggca tgatcagcgt gcccagcacg gtgccttcga
      961 agcccttgct ctccatcgcg tgcagacgcc agatcatcac gaagctggcc accacgaaca
     1021 gggccagcag catcagggga tgggcgaaat agccctggat attttcgatt gcgtttttca
     1081 cagcggacat aaggaatgaa gcgggcggat tctaccttgc tgaacggaca tggcaacttt
     1141 gtcacccctg atgatgaaac ttgccatgcc cgtttccccc tatccaactt tcccccgcac
     1201 gcgggataac cagcgacttg gctgcggttg tttgcagcct agtcgaatgg ctagttgcct
     1261 cacccagagg attgaggaga gggaacgggc ttggcaagtt tcataatcag aaggccatgc
     1321 ccgttcggta taatccgcgc catgccttcc atacacctgc tccccgactt actcatcaac
     1381 cagattgccg ccggcgaagt gatcgagcgc cccgcctccg cgctgaaaga gctgatggag
     1441 aacagtctgg atgcgggcgc ttccgacatc gcggtgcagc tcgaaggcgg cggcatcaag
     1501 ctgctgcgca tccgcgacaa cggccgtggc atcgcaaagg aacagctggc gctggcgctg
     1561 atgcgccatg ccaccagcaa gatcgccagt ctggaagacc tgcagaaagt cgccagcatg
     1621 ggctttcgcg gcgaggcgct ggccagcatg gcggcggtcg cgcagctgac cctgactagc
     1681 agaacaacaa cgcccagccg caccgcagag gacactcatg gctggaagat cgaggccatc
     1741 gacggccggc tcagcgagcc tgccccgacc agccagccgc agggcaccac ggtggaactg
     1801 cgcgagctgt atttcaacac cccggcgcgg cgcaaatttc tgaagagcga agccaccgag
     1861 ttcgcctggt gcgacgaggc cttcaagcgc atcgcgctgt ctcgccccga cgtcgccttc
     1921 tcgctgcagc acaacggcaa gaccatccgc cagctgcccg tgcaatccat cgccgcgcgc
     1981 atagctgcga tcctgggcgc cgagttcggc caggccgccg tggtggtcga acggcaggcg
     2041 gcgaacctgt cgatgcaggg tctggccgcg ctgcccgcct attcgcgcag ttcgcgcgac
     2101 gcgcagtatt tcttcgtgaa cggccgcttc gtgcgcgaca aggtcgccag ccacgcattg
     2161 cgtcaggcct accaggacat cctgcaccac cagcgccacc cggccttcgt gctgttcctc
     2221 gacatgccgc cggaacaggt ggacgtgaac gtgcacccgg ccaagagcga ggtgcgcttc
     2281 cgcgagagcc agggcatcca tcagttcatc ttccacacgc tgcaagaatc gctgagcgtg
     2341 acgatgggcg aatgcgaagc cccggcgcag accgtcgcac cgaccaacac ctatgtcgcg
     2401 ccccagttcc ggcagcaagc catgacgctg ggcgtcgcag agcgcgaggc cacctaccga
     2461 ttgtgggaaa cacaaacagg aagtagtcag tcggaagtag taagtggtga gtggagagag
     2521 cgtagcgagg gacagggaag tggaaaaacc accgcagccc cctactcacc actgaccact
     2581 gaccacttac catcgccaac cgaccactca ccagccaaca ctcacccctt gggctacgcg
     2641 atcggccagc tctccggcgt ctacatcctc gcgcagaaca cgcagggcat gatcgtggtg
     2701 gacatgcacg ccgcgcacga gcgcatcgtc tacgagcgcc tcaaggccgc actcgatgcc
     2761 gaacgcatcg cctcgcagcc gctgctgatc ccggtgagct tctatgccga accgctggat
     2821 gtcgcgaccg tggaggcgga acaggaagcg ttgcacaagc tgggcttcga cttagcgccg
     2881 ctgtcgccca cccagctcgc gctgcgcgcg atgccgacga tgctcaagca atcggaggcc
     2941 gagaccatgg cgctggaagt gctgcgcgaa ctgcgcgaat acggcgcgag ccgcgcgctg
     3001 accgaacggc gcaatgaact gctagccagc ctcgcctgcc acgccgccgt gcgcgccaat
     3061 cacgccttaa gcctcaccga gatgaacgcg ctgctgcgcg aaatggagca gaccgaacgc
     3121 gccgaccagt gcaaccacgg ccgcccgacc tggttccaga tgaccatggg cgatctcgac
     3181 aagatgttca tgcgcggaaa atagcgacac acacgaggtg ggagcggctt cagccgcgaa
     3241 tagcacacaa atcgcggctg aagccgctcc cactgaaaac accgacagga ttaccaagga
     3301 tttatccagt gacagcctcc ccttcgatct tccggatcgc cttcgccagc ttcatcggca
     3361 cggccatcga gttctacgac ttctacattt acggcctcgc ggtcgcgatg gtgatcgggc
     3421 cggtgttctt ccccaacgac gacccggcgg tgcaggcgct gaacgccttc ctgaccttcg
     3481 gcatcgcctt cctcgcgcgc ccgatcggcg cgctggtgtt cggccatttc ggcgaccgtg
     3541 tggggcgcaa ggccacgctg gtcgcatcct tgctggtgat gggcatctcc accaccctga
     3601 tcggcgtgct gccgagttac gagatggtcg gctggaccgc acctctgctg ctgtgcctgc
     3661 tgcgcttcgg ccagggcatc ggtctcggcg gtgaatgggg cggggcggcc ttgctcgcgg
     3721 cggagcatgc gcccaagggg cggcgcggct ggttcggcat gttcccgcag ctagggcccc
     3781 cggtcggctt catcttcgcg gtcggcggct tcctgctgct gtccagcctg ctgaccgacg
     3841 aacagttccg cagctgg
//
LOCUS       DASUIV010000137         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_868119, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000137 DASUIV010000000
VERSION     DASUIV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868119"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            506..2071
                     /locus_tag="VFW59_05140"
     CDS             506..2071
                     /locus_tag="VFW59_05140"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX5363630.1"
                     /translation="MIGIPRETAAGEKRVATVPEVVEKLIKLGFRVAVESGAGEGANC
                     SDDSYRAAGAEIMDSAAALWVASDIVFKVVAPTSAEVELMREGITLIGFIWPAQNPEL
                     MQQLAAKRATVLAIDALPRMLSRAQKMDALTSQAGVAGYRAVIEAANAFGRFFNGQIT
                     AAGKVPPAKVFIAGAGVAGLAAIGTAAGLGAIVRANDTRAEVADQVKSLGGEFVKVEY
                     EEEGGGGGGYAKVMSEGFQQAQRDMYAKQAREVDIIITTALIPGKPAPRLITAEMVQS
                     MKPGSVIVDMAAERGGNCELTEPGRAVVKHGVTIIGYTDLNSRLAKQSSSLYATNLFR
                     LTEELCKTKDGIINVNMEDDAIRGLTVIKDGEITWPAPAPKLPPAKPAAAVKAAPAPA
                     HGQGGAPASAGKTAAIFGAAALLFLLIGANAPAAFLGHFTVFVLACFVGYMVVWNVTP
                     ALHTPLMSVTNAISSIIAIGALVQIAPPLLVGLDRPEEWIRWLAVAGIALTAVNMFGG
                     FAVTRRMLSMFRK"
     gene            2084..>3348
                     /locus_tag="VFW59_05145"
     CDS             2084..>3348
                     /locus_tag="VFW59_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5363631.1"
                     /translation="MSASLATVSYIGATILFILSLGGLSSPESSRRGNLYGMTGMTIA
                     VLATVFGPRVSMAGIPWILAAMVVGGSVGLYAARKVQMTQMPELVALMHSLVGLAACL
                     VGFANYIDPMAALNLSSAEKAIHEIEIYVGILIGAVTFAGSLIAFGKLSGKIGGKPLL
                     LPGRHFMNLAGLLVVAAFGYQFLHAETISAGMIALTVMTVIALLFGIHMVMAIGGADM
                     PVVVSMLNSYSGWAAAATGFMLNNDLLIVTGALVGSSGAILSYIMCRAMNRNFFSVIA
                     GGFGGGAPAKKASGAAEPAGEVIAISATETAELLRDAKSVIIVPGYGMAVAQAQHTVN
                     EITQFLREKGVNVRFGIHPVAGRMPGHMNVLLAEARVPYDIVMEMDELNGDFPDTDVA
                     IVIGANDIVNPAAQEDPTSPIAGMPVLEV"
BASE COUNT          642 a          962 c         1084 g          660 t
ORIGIN      
        1 gcgcccgcat ccagactgtt ctccatcagc tctttcagcg cggaggcggg gcgctcgatc
       61 acttcgccgg cggcaatctg gttgatgagt aagtcgggga gcaggtgtat ggaaggcatg
      121 gtgcggatta tcttaaaaaa cagttgtccg cagattacac agatttcaac tgattaaaca
      181 ataaactatc tcgttaatcg gagcgccttt cgggcgagac attcgtcgag catttagtgc
      241 atgagaatct gcgttaatct gtggaatctg cggatggaac ttgggtttcc aagattatac
      301 ataatgcccc cgcgtttact tggccgtcat gtatcgcgcg aagcggagcg tgttgcttgc
      361 cgggtttttg aacgaacaat gccgcgcagt tgggaaaaga gctcgtatat aatcgcggag
      421 atattcttat agaaaaacat tcccccgttc atacgggcat tttccagaac aacgtcaaca
      481 gttataggaa gactacatgg ctctaatgat aggaattccc cgtgagacag ccgcagggga
      541 gaagcgggtt gcgacggtgc cggaagtcgt agagaagctt atcaagctgg gtttcagagt
      601 cgcggtcgag tctggcgccg gagagggcgc gaattgcagc gacgacagtt accgcgccgc
      661 aggcgccgaa atcatggaca gtgcggcggc cctgtgggtg gcatccgaca tcgtgttcaa
      721 ggtggtcgcc ccgacgagcg ccgaggtcga attgatgcgc gaaggtatca ccttgatcgg
      781 tttcatctgg cctgcgcaga atccggagct gatgcagcaa ctcgcggcga agcgcgccac
      841 cgtgctggcc atcgacgcgc tgccgcgcat gctttcccgc gctcagaaga tggacgcgtt
      901 gacttcacag gcgggcgtcg ccggataccg cgccgtgatc gaggccgcca atgccttcgg
      961 ccgcttcttc aacggccaga tcacggctgc gggcaaggtg cctccggcca aggtgttcat
     1021 cgcgggtgcc ggtgtggccg gtctggccgc gattggcacc gccgcagggc tgggcgcgat
     1081 cgtgcgcgcc aacgataccc gtgccgaagt ggccgatcag gtcaaatccc tgggcggcga
     1141 gttcgtgaaa gtcgagtacg aggaggaggg cggcggtggc ggcggctatg ccaaggtgat
     1201 gagcgagggc ttccagcagg cccagcgcga catgtacgcc aagcaggccc gcgaggtgga
     1261 catcatcatc accaccgcgc tgattcccgg caagcccgcg cccagactga tcaccgcaga
     1321 gatggtgcag agcatgaagc cgggcagcgt catcgtcgac atggccgccg agcgtggcgg
     1381 caactgcgag ctgaccgagc caggccgtgc agtggtgaag cacggcgtga ccatcatcgg
     1441 ttacaccgat ctgaattcgc gtctggccaa gcagtcttcc tcgctgtatg caaccaacct
     1501 gttccgcctg accgaagaac tgtgcaaaac gaaggacggc atcatcaacg tcaacatgga
     1561 agacgatgcg attcgcggcc tgacggtgat caaggacggc gagatcacct ggcctgcgcc
     1621 cgcaccgaag ctgcctccgg ccaaaccggc ggcagccgtc aaagcggctc cggctccggc
     1681 gcatggacag ggtggcgcac ctgcctcggc agggaagacg gccgcgatat tcggtgccgc
     1741 cgcgctgctg ttcctgctga tcggtgccaa tgcgcccgca gcgttcctcg ggcacttcac
     1801 cgtgttcgtg ctcgcgtgtt ttgtcggtta catggtggtg tggaatgtca ccccggcgct
     1861 gcacactccg ttgatgagcg tcaccaacgc gatttccagc atcatcgcga tcggcgcact
     1921 ggtgcagatc gcgccgccgc ttttggttgg actggatagg ccggaggaat ggatacgctg
     1981 gctggcggtg gcgggtatcg cgctcactgc ggtcaatatg ttcggcggct tcgccgtgac
     2041 ccgacgcatg ctgtcgatgt tccgcaaata aggataaatg aatatgtctg caagcttggc
     2101 aacggtttct tatattggtg cgaccatact cttcatcctc agcctgggcg gtctgtccag
     2161 ccccgagtca tcgaggcgcg gcaaccttta cggcatgaca ggcatgacga ttgccgtgct
     2221 ggcgacggtg ttcggcccgc gtgtttcgat ggccggcatt ccctggatcc tcgccgcgat
     2281 ggtggtgggc ggcagcgtcg gactctatgc ggcgcgcaag gtgcagatga cgcagatgcc
     2341 ggaactggtc gcgctgatgc acagtctggt ggggctcgcc gcctgtctgg tcggcttcgc
     2401 caattacatc gacccgatgg ctgcattgaa tctgtccagt gccgagaagg cgatccacga
     2461 gatcgagata tatgtcggca tcctgatcgg tgcggtgact ttcgccggtt ccctgatcgc
     2521 cttcggcaaa ctgtcgggca agatcggcgg caagccgctg ttgctgccgg gacgtcactt
     2581 catgaatctg gcaggcctgt tggtcgttgc cgcgttcggt taccaattcc tgcatgccga
     2641 aacgataagt gcgggcatga tcgcgctgac ggtgatgacc gtgatcgcct tgctgttcgg
     2701 catccacatg gtgatggcga tcggcggtgc cgatatgccg gtggtggtgt ccatgctgaa
     2761 cagctactcc ggctgggcgg cagcggcaac cggcttcatg ctgaacaacg atctgctgat
     2821 cgtcaccggc gcgctggtgg gttccagcgg cgccatcctg tcctacatca tgtgtcgcgc
     2881 gatgaaccgc aacttcttca gcgtgatcgc gggcggattc ggtggcggcg cccctgccaa
     2941 gaaggccagc ggtgctgcgg aaccggcggg cgaagtgatc gctatcagcg cgaccgagac
     3001 ggcagagctg ctgcgcgatg cgaagagcgt gatcatcgtg ccgggctacg gcatggcggt
     3061 ggcccaggcg cagcacacgg tcaacgagat cacgcagttc ttgcgcgaga agggcgtcaa
     3121 cgtgcgcttc ggcatccacc cggtcgccgg ccgcatgccc ggtcacatga acgtgctgct
     3181 ggccgaagca cgagtgcctt acgacatcgt gatggagatg gatgaactca atggggactt
     3241 cccggatacc gatgtcgcca tcgtgatcgg tgccaacgac atcgtgaacc cggccgcgca
     3301 ggaagacccg accagcccca tcgccggcat gccggtgctg gaagtctg
//
LOCUS       DASUIV010000138         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_590386, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000138 DASUIV010000000
VERSION     DASUIV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590386"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2037
                     /locus_tag="VFW59_05150"
     CDS             <1..2037
                     /locus_tag="VFW59_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5363632.1"
                     /translation="YEIEHRLLMADGRIKYVHERCETIYAEDGKPLRSTGTVQDISAR
                     RHVEEALRASEEKIRSLFELSPLGIALTDMQGRYVEFNEAFRAICGYPADELVALDYW
                     TLTPREYEASEALQLEALSTTGRYGPYEKEYRQKDGRRIPIQLNGVLVTGRDGQQYIW
                     SIVEDITERKRSEVLLTERLAHIQELNLHLEENARNLEEQTVELEASQEQIKQTEEWY
                     RSILHSAPDGMMVVDDRGVIMQVNERLAAMFGYQEVELLGCNIEILLPRALRESHVGM
                     RNSFLARKEGHRDAMVGKALFGCRKDGLEFPVDVSLSRLPELEGRRRAICASVRDVSQ
                     RHAMEVAREEALAEALRLAQLRSAFMAQMSHELRTPLNGILGHAQNLLHGGALAEKQI
                     GGLNIIQHSGEHLLSLINGILDHAAIEADKFELIPGDIQLETFLSTIIGIIRVRVEQK
                     NLGFSCVVDANLPAVVRGDAQRLRQVLLNLLANAVKFTDRGQVSLRVDYTAPQRLRFE
                     VQDSGVGIAADQLENIFLPFEQIGETRHRAGGTGLGLAISRKLVRLMGGDIKVESQAG
                     AGSTFWFEIEMESEQSDSARVNAAALSEQAETRVAQATMTLSVPPRQELDILHGLALR
                     GSMRDIMRHAENLAEADGRYLPFVEQLRQLAKNFQTKALLGLIEQYRSEQEIES"
BASE COUNT          477 a          636 c          734 g          401 t
ORIGIN      
        1 tacgagatcg aacatcgtct gctgatggcg gacgggcgca tcaagtacgt ccacgaacgc
       61 tgcgaaacca tttatgccga ggacggcaag ccgctgcgct cgacgggcac ggtgcaggac
      121 atctccgcgc gcaggcatgt cgaagaagcc ttgcgcgcca gcgaggagaa aatccgcagc
      181 ctgttcgaac tctcgccgct cggcatcgcg ctgaccgata tgcagggccg ctatgtcgag
      241 ttcaacgagg ctttccgcgc gatctgcggc tatccggcgg atgaactggt ggcgctggat
      301 tactggacgc tgacgccccg ggaatacgag gcgagcgagg ccttgcaact ggaggccttg
      361 tctacaaccg gccgttacgg cccttacgag aaagagtacc ggcagaagga cggcaggcgc
      421 atcccgatcc agttgaacgg cgtgctggtc accggccgcg acgggcagca gtacatctgg
      481 tccatcgtcg aggacatcac cgaacgcaaa cgttcggagg tgctgctgac cgagcgtttg
      541 gcgcatattc aggagctcaa tctccatctg gaagagaatg cgcgcaatct ggaagagcag
      601 acggtggaac tggaggcctc gcaggaacag atcaaacaga ccgaggaatg gtatcgcagc
      661 attctgcatt ccgctcccga cggcatgatg gtggtggacg accgtggcgt catcatgcag
      721 gtcaatgagc gccttgccgc gatgttcggc tatcaggagg tcgagttgct gggttgcaac
      781 atcgaaatct tgctgccgcg cgccttgcgc gaaagccatg tggggatgcg caacagcttc
      841 ctcgccagaa aggaagggca tcgcgatgcc atggtcggca aagccttgtt cggctgccgc
      901 aaggatggtt tggaattccc ggttgacgtg agtctgtcgc ggctacccga actggaaggc
      961 aggagacgcg ccatctgcgc ttcggtgcgc gacgtcagcc agcgtcatgc gatggaagta
     1021 gcgcgcgaag aggcgcttgc ggaagccctg cgtctggcgc aattgcgcag cgccttcatg
     1081 gcgcagatga gccatgaact gcgcaccccg ctgaacggca tcctcggtca cgcccagaac
     1141 ctgctgcacg gcggcgcgct ggctgaaaaa cagatcggcg ggctgaatat catccagcat
     1201 agcggcgagc atttgttgtc gctcatcaac ggcatactcg atcacgccgc gatcgaagcg
     1261 gataaattcg agctcatccc cggcgatatt caattggaga ctttcctgtc cacgatcatc
     1321 gggattatcc gcgtcagggt cgagcagaag aacctgggat ttagctgcgt cgtggatgcc
     1381 aatctgcctg ccgtggtcag gggcgatgcc cagcgtctgc gccaagtctt gctcaacctg
     1441 ctggcaaacg cggtcaaatt caccgaccgc ggacaggtga gcctgcgggt cgattacacc
     1501 gctccacagc ggctgcgttt tgaggtgcag gacagcggcg tcggtatcgc cgccgatcaa
     1561 ctggagaaca tcttcctgcc gttcgagcag atcggcgaaa cccgccatcg tgccggcgga
     1621 acggggttgg ggctggcgat cagccgcaag ctggtgaggc tgatgggcgg cgatatcaag
     1681 gtcgaaagcc aggccggtgc ggggagcact ttctggtttg aaatcgagat ggagagcgag
     1741 cagtccgatt cggccagagt caatgcggcg gccttgtcgg aacaggcgga aacgcgtgtc
     1801 gcacaggcaa cgatgacgct gtcggtaccg ccccggcagg aactggacat cctgcacggg
     1861 ctggcactgc gcggcagcat gcgcgacatc atgcgccatg cggaaaatct ggccgaggcg
     1921 gacggccgct atcttccgtt tgtcgagcaa ctgcgccagt tggcgaaaaa cttccagacc
     1981 aaggcactgc tgggcctgat agagcaatac cgcagcgaac aggaaatcga atcatgaagc
     2041 agcctgtttt cataggagcg gcggtttttg taggagcggc ttccacccta aagggtacga
     2101 gagccgcgaa ggggatgcgg atacgcaacg tgtccatcgc ggctcaagcc gctcccacgg
     2161 ggtgcagcgg atttgcgtag gagcggcttt caccctgaag ggtacgagcg ccgcgaaggg
     2221 attgggacat gcaacgtgtc catcgcgg
//
LOCUS       DASUIV010000139         7092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_590524, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000139 DASUIV010000000
VERSION     DASUIV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590524"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFW59_05155"
     CDS             <1..481
                     /locus_tag="VFW59_05155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206516.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5363633.1"
                     /translation="ELGVLPAVDVGKSVSRVGGKAQRAAYRSVAGDLKLAYAQFEELE
                     TFARFGARLDEDTRKIIEHGRRIRACLKQPEFTPVPVAEQIAVLLALTAGLFDPVPLE
                     QMGDAELAVRKAAAEIPAEVRARFDTADRLGDEDRSSIIEIARQALASFQPEPEGGS"
     gene            478..1380
                     /locus_tag="VFW59_05160"
     CDS             478..1380
                     /locus_tag="VFW59_05160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX5363634.1"
                     /translation="MSETAASLRHKIASAKDLESVVRTMKAMAASNIGQYENAVRSLG
                     DYYRTVQLGLAASLKQGEPTAFAGQGEDNGPAGAIVFGSDQGLVGQFNEVMAAFVEEK
                     LGSLPGKKTVWAVGERIHARLAEAGLSPVSGFELPNSINAITPLVGKILIELELRRER
                     GEIGQVYLFHNRPKSGSIYSSVSQRLLPLDALWQRDLSAISWPTGSLPEIVGGGDQTI
                     RALVSEYLFVSLFSACAESLASENASRLAAMQRAEKNIDELRESLNRTFHRLRQSGID
                     EELFDVISGFEAMTVGQGKHKGAG"
     gene            complement(1439..2266)
                     /gene="galU"
                     /locus_tag="VFW59_05165"
     CDS             complement(1439..2266)
                     /gene="galU"
                     /locus_tag="VFW59_05165"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX5363635.1"
                     /translation="MITKCLFPVAGYGTRFLPATKAQPKEMLPILTKPLIQYGVEEAL
                     DTGMNHIAIVTGRNKRAIEDHFDVTYELEDQISGTSKENQLDCIRDLIDRCTFSYTRQ
                     AYMRGLGDAILTGETLIGDHPFGVILADDLCVGEPDGVMAQMVKVYEKYRCSIVAVEE
                     VPANEVDKYGIVSGQQLEEGLIQVEHMVEKPSPQDAPSNLAVIGRYILTPDIFDILRN
                     TPPGKNGEVQLTDALQTQAREGKVIAYRFHGRRFDCGSIEGFVEATNFFFNRFKEAH"
     gene            2543..3481
                     /locus_tag="VFW59_05170"
     CDS             2543..3481
                     /locus_tag="VFW59_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX5363636.1"
                     /translation="MNALICGSMAYDTIMVFPDHFKKHILPEQLHILNVAFLVPTMRR
                     EYGGCAGNIAYNLQLLGGKPLIMATVGDDFAPYAKRLEKLGISQQHIRQVPDSFTGQA
                     FITTDLDDNQITAFHPGAMGFSHQNKVADASDVSIGIVSPDGRDGMMEHAEQFVEAGI
                     PFVFDPGQGMPMFSGDDLMRFVDQATYVTVNDYEAKLLQDKTGKTLKQIAGSVKALIV
                     TLGGDGSMIYADGREIAIPTPKPASIVDPTGCGDAYRAGLLYGIQSGWDWETTGRLAS
                     MMGSIKIASRGGQNHAPTRDELKAQFQKHFGFALPL"
     gene            3585..4058
                     /locus_tag="VFW59_05175"
     CDS             3585..4058
                     /locus_tag="VFW59_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363637.1"
                     /translation="MPFRVKVVALLAAASLLAGCAGEPQVAPKEVVQYGVIESVTPIK
                     LDDSSNVGTNVGGIFGQAGGASSGGGSGAAVGSIFGAVLGSTLGRQAGIATKPGLEIW
                     VKLDESGKSTYVMQPGKPDAFKVGDPVRVIRKGKAARVEPSLLGAPPAAEPPKEQ"
     gene            4055..4939
                     /locus_tag="VFW59_05180"
     CDS             4055..4939
                     /locus_tag="VFW59_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX5363638.1"
                     /translation="MTDWPAISAAIGAATGQAFQLINPDRVGGGCISQAYRLNGRDGR
                     SFFVKLNAADKFEVFIAEAAGLSEIAATGTVRVPQPVTQGQCGAHSFLVLEHLELNGR
                     GREGQLGERLAALHRVTATHFGLARDNFIGTTRQPNGWMQDWPTFWREQRLGFQLQLA
                     ARNGYGGRLQTLGAQLLERLPAFFDGYAPLPSLLHGDLWSGNRAYLPDGTPLIFDPAA
                     YYGDRECDLAMTELFGGYGADFYAAYRAAYPPDAGYAVRRDLYNLYHILNHANLFGGG
                     YVRQAEGTAQRLLAELDC"
     gene            5119..5322
                     /locus_tag="VFW59_05185"
     CDS             5119..5322
                     /locus_tag="VFW59_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5363639.1"
                     /translation="MATGSVKWFNDSKGFGFITPENGGDDLFAHFSAIQGEGFKTLTE
                     GQRVTFDITTGPKGQQASNIRGA"
     gene            5327..5590
                     /gene="infA"
                     /locus_tag="VFW59_05190"
     CDS             5327..5590
                     /gene="infA"
                     /locus_tag="VFW59_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5363640.1"
                     /translation="MAKEELLEMNGVVDEVLPDSRYRVTLENGHTLIAYSAGKMRKNH
                     IRIIAGDKVSLELSPYDLSKGRITFRHIEGRTPGGGFQQRRRY"
     gene            complement(5658..6479)
                     /locus_tag="VFW59_05195"
     CDS             complement(5658..6479)
                     /locus_tag="VFW59_05195"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEX5363641.1"
                     /translation="MATYAIGDVQGCYTELVQLLEAIRFDTGTDRLWLVGDLVNRGPG
                     SLEVLRLVKSLGDSAITVLGNHDLHLLAVAAGAAELHRSDTLNEILAAPDRDELLHWL
                     RNQRLLHAEGKHVLVHAGLLPNWTVKQALKLAHEVETALRGDDYATFLARMYGNSPHG
                     WDDSLTGQKRLRVITNALTRMRICTDDGEMEFKFKGEIQDIPPGYRPWFEVPKRASSE
                     ATVIFGHWSALGLMVSKNAIALDTGCLWGGPMSAIRLEDRELFQVECNNPVAKHW"
     gene            6679..>7092
                     /locus_tag="VFW59_05200"
     CDS             6679..>7092
                     /locus_tag="VFW59_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5363642.1"
                     /translation="MKPIPFNKPFIIGRELALIADAVSRGHLSGDGHYTQLCSRWLET
                     QLNCRKALLTHSCTAALEMAAILCDLQPGDEVILPSYTFVSTANAFALRGAVPVFVDI
                     RSDTLNIDEKLIEAAITSKTRVIVPVHYAGVACEMD"
BASE COUNT         1344 a         2023 c         2204 g         1521 t
ORIGIN      
        1 cgagttgggc gtgctgcctg cggtcgatgt cggcaaatcc gtctcgcgtg tcggtggcaa
       61 ggcgcaacgc gcggcctatc ggtcggtcgc tggcgacctc aagctggcct acgcccagtt
      121 cgaagagctg gagacttttg cacgcttcgg tgccagactg gacgaggaca cgcgcaagat
      181 catcgaacac ggacggcgca tccgcgcctg tctcaaacaa cccgaattta cgccggtgcc
      241 ggttgccgag caaatcgccg tgctgctggc attgaccgcc ggactcttcg atcccgtgcc
      301 gctggagcag atgggcgatg cggaactcgc cgtgcgcaag gcggcggcag agattccggc
      361 cgaggtgcgc gcgcgcttcg atacggctga caggttgggc gacgaggaca ggagctcgat
      421 catcgagatc gcccggcaag cgctcgcgtc atttcagccc gagcccgaag gcggttcatg
      481 agcgaaacgg ctgccagtct gcgtcacaag atcgccagcg ccaaggacct cgaatccgtg
      541 gtgcgcacga tgaaggccat ggcagcctcg aacatcggcc aatacgaaaa tgcggtgcgc
      601 tctctgggcg actattaccg gacggtgcag ctgggtctgg ctgccagcct gaaacaaggt
      661 gagccgaccg cctttgcggg gcaaggtgag gacaacgggc cggcaggcgc catcgtgttt
      721 ggatccgatc aggggctggt cggacagttc aatgaggtga tggcggcgtt cgtcgaggag
      781 aagctgggca gcctgccggg aaagaagacg gtatgggcgg tcggcgaacg catccatgcc
      841 cgtcttgcgg aagccggcct gtcaccggtt tcgggttttg agctgccgaa ttccatcaac
      901 gcgatcacgc cgctggtcgg gaagattctg atcgaactgg aactgcgccg cgaaaggggc
      961 gagatcgggc aggtctatct cttccataac cggcccaagt ccggatcgat ctattcctcc
     1021 gtgagtcaac ggctgctgcc gctggatgcg ttgtggcagc gcgatctgtc ggctatctcc
     1081 tggccgacag gcagcctgcc cgagatcgtg ggcggcggcg atcaaacgat acgggcgctg
     1141 gtcagcgaat atttgttcgt ctcgctattc agtgcctgtg cggaatcgct ggccagcgaa
     1201 aatgccagcc gtttggccgc gatgcaacgc gcggaaaaga atatcgacga attgcgggaa
     1261 agcctgaacc gcacatttca ccgcctgcgt caaagcggga tcgacgagga gctgttcgat
     1321 gtcatttccg gctttgaggc gatgaccgtg gggcaaggaa aacataaagg agcgggctga
     1381 gcctgatgca aaagattgtt ggataaaggg cgagccttgt ccatgttccc atgacgattc
     1441 agtgcgcttc cttgaaccgg ttgaaaaaga agttggtggc ttcaacgaag ccttcgatgc
     1501 tgccgcaatc gaagcgccgt ccatgaaagc gataggcgat taccttgcct tccctggcct
     1561 gggtttgcag cgcatcggtg agttgtacct cgccgttctt gccgggcggg gtgtttctca
     1621 ggatgtcgaa aatgtcgggg gtcagaatat agcgcccgat cacggcgagg ttggatggcg
     1681 catcctgcgg cgacggtttt tctaccatgt gttcgacctg aatcaggccc tcttccagtt
     1741 gctgcccgct gacaatgccg tatttgtcga cttcgttggc ggggacttct tcaacggcga
     1801 cgatgctgca acgatatttc tcgtagacct tcaccatttg cgccatgacc ccgtcgggtt
     1861 cgcctacgca caggtcatcg gccagaatca cgccgaacgg gtggtcgccg atgagcgtct
     1921 cgccggtcag gatggcatcg ccgaggccgc gcatgtaagc ctgtcgcgta taggaaaagg
     1981 tgcagcggtc gatcaggtcg cggatacagt cgagttggtt ttccttgctg gtaccgctga
     2041 tctggtcttc cagttcgtag gtgacgtcaa agtggtcttc gatcgctcgc ttgttgcgtc
     2101 cggtgacgat ggcgatatga ttcatgccgg tgtcgagcgc ttcttccacg ccatactgga
     2161 tgaggggttt ggtgaggata ggcagcatct ctttcggttg tgccttggtc gcggggagga
     2221 aacgggtgcc gtaaccggcc accggaaaga ggcatttcgt gatcatgttc gtgtccttgg
     2281 ggaaatcggt cagccaataa atagagccgc agtggcaatc gtaacttttt ttgttgtgat
     2341 gcgggtgttc gtggaaacat tctgatgttc tgccggaaag cgtcatgccg ttctggcgtg
     2401 tcggccgtta atctctgccg atgccaccga gtgccagcgc cctgttctgc gaagcggttg
     2461 atgggttcga tctcaagagg ctctgcggcg gcgggcggcg attgctataa tccaagcttc
     2521 ttacacacgc aaattcgaca ttatgaacgc tctcatctgc ggctccatgg cttacgacac
     2581 catcatggtg ttccccgatc acttcaagaa gcacatcctg cccgagcagt tgcacatcct
     2641 gaatgtggcg ttcctggtgc cgacgatgcg ccgcgaatac ggcggctgtg cgggcaacat
     2701 cgcctataac ctgcagctgc tgggcggcaa gccgctgatc atggcgacgg tgggcgacga
     2761 cttcgcgccc tatgccaagc gcctggagaa gctgggaatt tcgcaacagc acatccgtca
     2821 ggtgccggac agcttcaccg gtcaggcctt catcaccacc gatctggacg ataaccagat
     2881 caccgccttc catcccggcg cgatgggttt ctcgcaccag aacaaggttg cagatgccag
     2941 cgatgtcagc atcggcatcg tgtcgccgga cgggcgcgac ggcatgatgg agcacgccga
     3001 gcagttcgtc gaagcgggta tccctttcgt atttgatccg ggtcagggca tgccgatgtt
     3061 ttccggcgac gacctgatgc gcttcgtcga tcaggcgacc tatgtcaccg tcaatgacta
     3121 cgaagccaaa ctgttgcagg acaagaccgg caagacgctc aagcagatcg ccggctcggt
     3181 gaaggcgctg atcgtcacgc tgggcggcga cggttcgatg atctatgccg acggccgcga
     3241 gatcgcgatc ccgacgccca agccggcatc catcgtcgat cctaccggct gcggcgatgc
     3301 ctatcgcgcc ggcctgctgt atggcatcca gagcggctgg gattgggaga ccaccggacg
     3361 cctggcgtcg atgatgggct cgatcaagat cgccagccgt ggcggtcaga accacgcgcc
     3421 gacacgcgac gaactgaagg cgcagttcca gaaacatttc ggtttcgccc tgccgctgta
     3481 atctgcctag cagaggttgc ttaacgatga acccggatag tcaatgcaaa ctcggggtat
     3541 gggtgggagc ggcttcagcc gcgatagtcc gtgcagtgaa tatcgtgccc tttagggtaa
     3601 aggtcgttgc gctgcttgcc gcagcatcgt tgctggccgg ttgtgccggt gagccgcagg
     3661 tggcgccgaa ggaggttgtc cagtacggcg tgatcgaatc cgtcacgccg atcaagctgg
     3721 acgacagctc gaatgtgggc accaatgtcg gcgggatttt cggtcaggcc ggtggtgcca
     3781 gcagcggcgg aggcagcggc gctgcggtcg gttcgatctt cggcgccgtg ctcggcagca
     3841 cgctggggcg gcaggcaggc atcgccacca agccggggct ggagatatgg gtcaagctgg
     3901 acgagagcgg caagagcacc tatgtgatgc agcccggcaa gcccgatgcc ttcaaggtcg
     3961 gcgatccggt gcgggtgata cgcaagggca aggcggcacg ggtcgagccg tctcttctgg
     4021 gggcgccgcc cgccgccgag ccgcctaaag aacaatgacg gactggcctg ccatctccgc
     4081 cgcgatcggt gcggcgaccg ggcaggcctt ccaactcatc aatcctgatc gggtcggcgg
     4141 cggctgcatc agccaggcgt atcgcctgaa cgggcgggat ggccgcagct tcttcgtcaa
     4201 gctcaatgcc gcagacaagt tcgaggtttt tatcgccgag gctgcggggc tgagcgagat
     4261 cgccgcgacc ggcacggtgc gcgtgccgca gccggtgacg caggggcaat gcggcgcgca
     4321 cagcttcctg gtgctggaac atctggagtt gaatggtcgc ggcagggagg ggcaactcgg
     4381 cgaacggctc gccgcgctgc accgcgtcac cgcaacgcac tttggtcttg cgcgggataa
     4441 cttcatcggc acgacgcgcc agccgaacgg ctggatgcaa gactggccga ccttctggcg
     4501 cgagcagcgc ctgggttttc agttgcagct ggccgcgcgg aacggttacg gcggcagact
     4561 gcaaacgctg ggcgcgcaat tgctggaaag gttgccggcc ttcttcgatg gttacgcccc
     4621 gcttccctcg ttgctgcacg gcgacctgtg gagcggcaat cgcgcctatc tgccggacgg
     4681 cacgccgctg atcttcgatc ccgccgccta ttacggcgac cgcgaatgcg atctggcgat
     4741 gaccgagctg ttcggcggct acggcgcgga tttctatgcg gcctaccgcg ccgcctatcc
     4801 gccggatgcg ggctatgcgg tgcgccgcga tttgtacaat ctctatcaca tcctcaacca
     4861 cgccaacctg ttcggcggcg gttacgtccg acaggccgag gggacagcgc agaggctgct
     4921 ggctgagctt gactgttaat ttcatttgct attctcccgg atacggggca tagtgcgggc
     4981 cgcgattttc aagcactctt gttgctgtct ggttcagtgt ccgcacttct gcggtatttc
     5041 tcccttcaac gccttgccgt cttgagcttc gttccgatca ggggctgttc cccttttata
     5101 tttcaggaga ttcaagatat ggcaacaggc agcgtaaagt ggtttaacga ttccaagggt
     5161 ttcggtttca ttacaccgga aaatggcggc gacgatttgt tcgcgcattt ctccgcgatc
     5221 cagggcgaag gtttcaagac attgaccgaa ggtcagcgtg tcaccttcga tatcaccact
     5281 ggccctaaag gtcagcaggc atccaacatt cgtggcgctt aaataaatgg cgaaggaaga
     5341 actgctcgag atgaatggcg tggtggacga agttctgccg gactcgcgtt atcgcgtcac
     5401 gctggagaat ggtcatactc tgatcgccta tagcgcgggc aagatgcgca agaaccacat
     5461 tcgcatcatt gccggcgaca aggtctctct ggagttgtcg ccctacgatc tgagcaaggg
     5521 gcgcatcacc ttccgtcata tcgaaggccg cactcctggc ggcggctttc agcaacgtcg
     5581 cagatattga tggcttgttt cgtgcccgcg ctctgcgggc atgaaacaag gcggtcttgg
     5641 ctccgtgctt cgtctgctta ccagtgtttc gccactgggt tgttgcattc aacttgaaat
     5701 aattcgcggt cttccagccg gatagcgctc atcgggccgc cccacaggca gccggtgtcc
     5761 agcgcgatgg cgtttttgct gaccatcagt cccagcgccg accagtggcc gaagatcacg
     5821 gtcgcttcac tgctggcgcg tttcggtacc tcgaaccagg ggcgatatcc cggcggtatg
     5881 tcctgaattt cccccttgaa cttgaattcc atctcgccgt cgtcggtgca gatgcgcatg
     5941 cgcgtcagcg cgttggtgat cacgcgcagg cgcttctgtc cggtgaggct gtcatcccag
     6001 ccatgggggc tattgccgta catgcgggcg aggaaggtgg cgtagtcgtc cccgcgcaat
     6061 gcagtctcga cttcgtgggc gagcttcagc gcctgcttca ccgtccagtt tggtagcagg
     6121 cctgcgtgta ccagcacgtg ttttccttct gcatgcagca ggcgctggtt gcgcaaccag
     6181 tgcagcaatt catcgcgatc cggtgcggcg agtatttcgt tcagggtgtc gctgcgatgc
     6241 aactccgctg cgcctgccgc gaccgccagc agatgcaggt cgtgattgcc cagcacggtg
     6301 atcgcgctgt cgcccagcga tttcaccagt cgcagcacct ccagcgaacc cgggccacga
     6361 ttcaccaagt cgccgaccag ccacagccgg tctgtacccg tatcgaagcg gatagcctcc
     6421 agcagttgca ctaattcggt gtagcagcct tgcacgtcgc ctattgcgta agtcgccatc
     6481 acgtacctca agctgtttat ctgtaaaata gcgtaccaga tttttatgtg attccgatga
     6541 aaagtgctga ttcatttcgg gaaaaatcct gtctatcgac ttgcgggcat gtcttgtaat
     6601 tgcgtagcta tgttgcgccg agacatttcc cggattgttt acctgtaaaa tggcgcttca
     6661 attttctacg tggttctaat gaaacccatc ccctttaata aaccttttat catcggccga
     6721 gagctggcgc tgattgccga tgcggtctcc agaggccatc tgtccggtga cgggcattac
     6781 actcagctgt gcagccgctg gttagaaacg cagttaaatt gtcgcaaagc attgctgacg
     6841 cactcctgta ctgcggcact ggagatggcc gcaatcctgt gcgatctgca gcccggtgat
     6901 gaggtaatcc tgccttccta taccttcgtc tcaacagcaa atgcctttgc gctgcgtgga
     6961 gcggttcccg tgttcgtcga catacgctct gatacgctga acattgatga gaaattgatt
     7021 gaagccgcaa ttacatcgaa gactcgggtg atcgtgccgg ttcattatgc aggtgtggca
     7081 tgcgagatgg at
//
LOCUS       DASUIV010000140         5952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1149634, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000140 DASUIV010000000
VERSION     DASUIV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149634"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /gene="sucD"
                     /locus_tag="VFW59_05205"
     CDS             <1..873
                     /gene="sucD"
                     /locus_tag="VFW59_05205"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5363643.1"
                     /translation="INKGTKVITQGMTGKVGQFHTFHCKQYANGRNCFVAGVNPKKAG
                     EDFEGIPVYATVADAKVATGATVSVIYVPPAGAAAAIDEAVDAGMDLVICITEGIPVH
                     DMLRTRYKMQGKRTLLIGPNCPGIITPDEIKIGIMPGHIHKKGRIGVVSRSGTLTYEA
                     VGQLSALGYGQSTCVGIGGDPINGMKHLDVMRLFNDDPDTDAVVMVGEIGGSDEEECA
                     RWIKANMRKPVVGFIAGITAPAGKRMGHAGAIISGGQGGAEDKLAVMEASGIHITRNP
                     AEMGRVMQQVLKGI"
     gene            874..1614
                     /locus_tag="VFW59_05210"
     CDS             874..1614
                     /locus_tag="VFW59_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5363644.1"
                     /translation="MMEMLLSTQFWVDVFKIIMIDLLLSGDNAVVIALACRNLPAAQR
                     KKGILYGVGGAIGLRVVLTFFAVTLLALPYLKLVGALLLLWIGVKLILPEDEHGEGSI
                     KAEGNLWGAVKTIIIADFVMSLDNVLGVAGAAHGNVWLLVFGLLISIPMIAWSSQLVL
                     KLIDRFPFIIYAGGALLGYVAGEMLVTEALLKPLVESQHYLHGLVPVAGALLVLSVGG
                     WLAMRKAVAIKAVDLVDATVTVKSNNEA"
     gene            1626..2051
                     /locus_tag="VFW59_05215"
     CDS             1626..2051
                     /locus_tag="VFW59_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5363645.1"
                     /translation="MKILIPVDGSPNALRAVEQAIKHAAALKQAPQLLLLNVQWNVAS
                     GNVKLFIDQRTIDDYYREQGESALASAREALDAAALPYQYHVSIGTPAEAIAQYADEQ
                     AVDQIIMGRQGQGGMQTLLLGSVVNKVLHQVSCPVTLVK"
     gene            2152..4755
                     /locus_tag="VFW59_05220"
     CDS             2152..4755
                     /locus_tag="VFW59_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5363646.1"
                     /translation="MDKLFWKKDWFVGLAVVLLFLLAAQSDLIQSLERKAYDLGMQAT
                     SRTPSDKIAVIAIDEESLRNLGRWPWPREIHARMVDILAEGHAKVVGHTAFFFEPQVD
                     AGLDYVRRIAGLLGKSALLDSAAPALGEQASMVSAASAVAADAEVAPETPVELTPLDV
                     LLQEAARNLDHDKKLSESMSKANDVLLAMFFELGEPQGKPDREQPDYVLRNSLPNVQD
                     AGHGTYPLPSRGVLSPIPALGSKALAIGHLNSFRDVDGAVRTEPLVVSYYDHFYPSLS
                     LLLAAKSLNLGPKDIQLILGAGVQLGNRKIRTDDASQMHTYFYKDQPGRPAFPVDSFY
                     DVLTGKIPAEKYRDKIVLIGATAAGVGALQVTPISSGMAPVVTLAHSVSSILQGDFFV
                     VPDWARWAESGAFLLVALYLVLLLPRLRAAIGAVFTLLLLAALLATHFVLMTRQGLWL
                     QLMLPAALLLVGHLLLTTKRFLITEQGKQKSDADSAESNRMLGLAFQGQGQLDIAFDK
                     FRKVPMDDSLMEVLYNLGLDFERKRQFNKAESVFKYMAEYNPKFRDLAQRLTQMHEMS
                     ETVILGGGSGGSNSSTMALGKSGVSKPMLGRYQIEKELGKGAMGVVYQGKDPKIGRVV
                     AIKTMALSQEFEGDELQDVMERFFREAETAGRLNHPNIVSIYDAGEEHDLAFIAMEFL
                     KGKDLMPYTKPGSLLPLSKVMSIVARVADALDYAHKQHVVHRDIKPANIMYDPETDTA
                     KVTDFGIARITDSSKTRTGMVLGTPSYMSPEQLAGKKIEGSSDLFSLGVSLYQLACGK
                     LPFEGDSMAQLMYRISNEPHTDIRSVRADIPPCLAAIVDKALAKDNESRYANGAEMAE
                     ALRQCASSLQG"
     gene            4759..5580
                     /locus_tag="VFW59_05225"
     CDS             4759..5580
                     /locus_tag="VFW59_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX5363647.1"
                     /translation="MRVDEALEIICQTDPGMVRSHNEDSVACEAACGLVVLADGMGGY
                     NAGEVASGIAVSVVETEICQRLKDASPIDRDEETGEELGEALLRDNILKANTSIFQAA
                     QSQPQYAGMGTTIVSGLFYDNKVVVGHIGDSRMYRLREGILESITRDHSLLQEQIDSG
                     MISREDARTSKNKNLVTRAVGIDAEVVPELHAYDVLVGDIYLLCSDGLNDMVEDDDIQ
                     STLYAMQGNLPLAAEQLIQLANDNGGRDNVSVILVKVKGEFAASRNWLEKLINWF"
     gene            5603..>5952
                     /locus_tag="VFW59_05230"
     CDS             5603..>5952
                     /locus_tag="VFW59_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5363648.1"
                     /translation="MAAKLILSMDGTVIKEYPLSKERMTIGRKSHNDIVIDNLAISGE
                     HAAIITILHDSFLEDLDSTNGVEVNGVAVKKHFLQNNDLIEIGKYKLKYINDQLSQTN
                     AADFEKTMVLRAPI"
BASE COUNT         1273 a         1443 c         1925 g         1311 t
ORIGIN      
        1 attaacaaag gcaccaaggt tattacccag ggcatgactg gcaaggtcgg ccaatttcat
       61 accttccatt gcaagcagta cgcgaacggc aggaattgtt ttgtcgccgg agtgaatcct
      121 aaaaaagccg gagaggactt cgagggtatt ccggtctacg ctaccgtggc agatgcaaag
      181 gtagcgacgg gcgccaccgt ttccgtgatc tatgtgccac ccgcaggagc ggcggcagcg
      241 attgatgagg cagttgatgc aggaatggat ttggtgattt gcatcaccga ggggattcca
      301 gtgcatgata tgttgcgtac ccgttacaag atgcaaggta agcgcacgtt gctgatcggc
      361 cccaactgcc ccggtattat cactccggac gagatcaaga tcggcatcat gccgggccac
      421 attcacaaga aaggccgtat cggtgtggta tcgcgttcag gcacgttgac ttacgaggct
      481 gtcgggcagt tgagtgcttt gggttacggt cagtccacct gcgtcggtat cggaggcgat
      541 ccgatcaacg gcatgaaaca tctggatgtg atgcgcttgt tcaatgatga cccggatacc
      601 gatgcggtgg tcatggtggg cgagatcggt ggtagcgacg aggaggaatg cgcgcgctgg
      661 atcaaggcca atatgcggaa gccggtggtt ggcttcatcg cgggtataac cgcccccgcc
      721 ggtaagcgta tggggcatgc cggtgcgatt atttccggtg ggcagggcgg ggccgaggac
      781 aagctcgcgg tgatggaagc gagcggcatc cacatcacgc gcaatccggc cgagatgggg
      841 cgcgtgatgc agcaagtatt aaaggggata tagatgatgg aaatgttgct ttctacccag
      901 ttctgggtgg acgtgttcaa gatcatcatg atcgatttgt tgctgtctgg agataacgcg
      961 gtggtgatcg cgctcgcttg ccgaaatctt cccgcagcac agcgcaaaaa aggcatcttg
     1021 tatggcgtgg gtggcgcgat cggcttgcgc gtcgtgctga ctttcttcgc ggtcacgcta
     1081 ctggcgttgc catacctcaa gctggtcggt gcgctcctgc tgctctggat cggcgtcaag
     1141 ctgatcctgc cggaagatga gcatggcgag ggcagcatca aggcggaggg aaatctgtgg
     1201 ggcgcggtga agaccatcat catcgccgac ttcgtgatga gtctggacaa tgtgctgggc
     1261 gtggcgggcg cggcgcatgg caacgtgtgg ttgctggtgt tcggtctgct aatcagcatc
     1321 ccgatgatcg cctggagcag tcagctggtg ctcaagctta tcgatcgctt tccttttatc
     1381 atttatgctg gcggtgcatt gctcggctat gtcgctggcg agatgctggt gacggaggca
     1441 ttgctgaagc ccttggttga gtcgcaacat tacctgcatg gcttggtgcc tgtggcgggg
     1501 gcgttgctgg tgctctcggt tggtggctgg ttggcgatgc gcaaggcggt cgccatcaag
     1561 gctgtcgatc tggtggatgc gacagtgacg gtcaagtcga ataacgaggc gtaagaggag
     1621 cagtgatgaa aatattgatt ccagtggatg gttcgcccaa cgccctgcgc gcagttgagc
     1681 aagccatcaa gcacgccgct gcattgaaac aggcgccgca gttgctgttg ttgaacgtgc
     1741 agtggaacgt ggcgagcggc aacgtcaagc tgtttatcga tcagcgaacc atagatgact
     1801 attatcgtga gcagggggag tcagcacttg catcggcgcg ggaggcactg gatgcggcgg
     1861 cattgcccta tcagtaccat gtcagcatcg ggacgccggc agaggcgatc gcgcagtatg
     1921 ccgacgagca ggcggtcgat cagatcatca tggggcggca ggggcaaggg ggaatgcaga
     1981 cactgttgct ggggtcggtg gtcaataaag tgctgcatca ggtgagttgc cccgtgacct
     2041 tggtcaaata gcatccttgt cggcgactgc agtccgccgc atgggaaggg aacgccacgt
     2101 acatcaatat cttctgcgtg aatcgcgcag gatgtgcatc agggagtgag gatggacaag
     2161 ttgttctgga agaaagactg gttcgtcgga ttggctgtgg tgctgctgtt tctgttggcg
     2221 gcccagagtg atttgatcca gagtctggag cgcaaggctt acgatcttgg aatgcaggct
     2281 acgtcccgca caccgagcga caagatcgcg gtgatcgcaa tcgatgaaga gagcctgcgc
     2341 aatctggggc gctggccatg gccgcgcgag atacacgcca ggatggtcga tattcttgcg
     2401 gagggacatg caaaggttgt cgggcacaca gccttcttct tcgagccgca ggtcgatgcc
     2461 ggattggatt atgtccgccg aatagccgga ttgctcggca agtcggcttt gctggatagc
     2521 gcggctcccg cgctgggcga gcaggccagt atggtgtccg cagccagtgc ggtcgccgca
     2581 gatgctgaag tggcgccgga gacgccggtg gagctgacac ccctggatgt tttgttgcag
     2641 gaagcggcgc gtaacctcga tcacgacaag aagctcagcg agagcatgtc caaggccaac
     2701 gatgttctgc tggcaatgtt tttcgagctg ggtgagccgc aggggaagcc ggatcgcgag
     2761 caaccggact acgtcttgcg caatagcctg cctaatgttc aggatgccgg gcatggcact
     2821 tacccgctgc cctctcgtgg cgtgttgtcg cctatccccg cgctgggcag caaggcgctg
     2881 gccatcggac atctgaacag ttttcgcgat gtggatggtg cagtgcgcac cgaaccattg
     2941 gtggtgagct attacgacca tttctatcca tcgctgtcct tgctgctcgc ggccaagagc
     3001 ctcaatctcg gcccgaagga tatccagctc attcttggtg cgggggtgca actgggtaac
     3061 cggaagatac ggaccgatga cgcatcacag atgcacacct atttctataa ggatcagcca
     3121 ggacgtccgg ccttccctgt cgattcgttt tacgatgtgc tcacgggcaa gatacctgcg
     3181 gaaaaatatc gcgacaaaat cgtgctgatc ggcgctacgg ctgccggggt aggggcgttg
     3241 caggtgactc ccatttcgtc aggcatggcg cccgtggtga cgctggcaca ttcggtgtcc
     3301 agcattcttc agggcgactt cttcgtggtg ccggactggg cgcgatgggc cgagtcgggg
     3361 gcgtttctgc tggttgcgct gtacctggtt ttattgttgc cacgtctgcg ggctgcgatt
     3421 ggtgcggtgt ttaccctgct gttgctggcg gcgctgctgg cgacccattt tgtactcatg
     3481 accagacagg ggctgtggtt gcagttgatg ttgccggcgg cgttgttgct ggtgggccac
     3541 ctgttgctga ccaccaagcg tttcctgata accgaacagg gcaagcaaaa atcggatgcc
     3601 gactcggcag agagcaaccg catgctcggg ttggcatttc aggggcaagg gcaactggat
     3661 atcgccttcg acaagttccg caaggtgccg atggacgaca gtctgatgga ggtgctgtac
     3721 aacctggggc tggacttcga gcgcaagcgc cagttcaaca aggccgaatc ggtgttcaag
     3781 tacatggccg aatacaaccc aaagttccgc gatctggcgc aacgtctgac tcagatgcat
     3841 gagatgtcgg aaaccgtcat ccttggcgga ggaagtggcg gctccaattc cagcacgatg
     3901 gcattgggta aatccggtgt gtccaagccg atgcttgggc gttatcagat cgagaaagag
     3961 ctgggcaaag gggcgatggg ggtggtgtat cagggcaagg atcccaagat cggtcgagtg
     4021 gtggcgatca agaccatggc cctgtcgcag gagttcgagg gcgacgagtt gcaggatgtg
     4081 atggaacgtt tcttccgaga ggccgagacg gcgggacgtc tgaatcaccc gaatatcgta
     4141 tccatttacg atgcaggcga agagcatgat ctggccttta tcgcgatgga attcctcaag
     4201 ggcaaggatc tgatgcccta taccaagccg ggcagtctgt tgcccctgtc caaggtgatg
     4261 agtattgtcg ctcgtgtggc cgatgcgctc gactatgcgc acaagcagca tgtggtacac
     4321 cgcgacatca agccagccaa tatcatgtac gatcccgaga cggatacggc caaggtcacc
     4381 gattttggca ttgcgcgcat caccgactct tccaagacgc gaaccggtat ggtgctcggc
     4441 acaccttcgt atatgtcgcc ggaacagttg gcaggcaaga agatcgaggg aagctcggat
     4501 ctgttctctt tgggggtgag tttgtaccaa ctggcttgtg gtaagctgcc gttcgaggga
     4561 gactccatgg cgcagctgat gtaccgtatt tccaacgagc cgcacaccga tattcgtagt
     4621 gtcagggcgg acatcccgcc atgcttggcg gctatcgttg acaaagcgct ggccaaggac
     4681 aatgaaagtc gttatgccaa cggcgcggaa atggccgagg cattgcgcca gtgtgcttcg
     4741 agtttacagg ggtagaacat gagagttgac gaggcgctgg agatcatctg ccagacggat
     4801 cccggcatgg tacggtcgca taacgaggat agcgtcgcgt gcgaggctgc ttgcgggctg
     4861 gtggtgctgg ccgatggcat gggtgggtac aatgccggtg aagtggcgag tggtatcgcg
     4921 gtctcggtcg ttgaaaccga gatatgtcag cgtctgaagg atgcgagtcc gattgatcgc
     4981 gacgaggaaa cgggtgaaga gctgggggag gcgctgctgc gcgataacat cctcaaggcc
     5041 aatacctcga tctttcaggc ggcacagagt cagccacaat atgcgggaat ggggacgacc
     5101 attgtttcgg gcctgttcta tgacaataaa gtcgtggtcg ggcatattgg cgattcgcgc
     5161 atgtatcgtt tgcgcgaagg cattctggag tctattacgc gtgaccattc gttgctgcag
     5221 gagcagatcg acagtggcat gatcagccgt gaggatgcgc gaacctccaa aaacaagaat
     5281 ctggtgacgc gggcggtggg cattgatgcc gaggtggtgc cggagcttca cgcgtacgat
     5341 gtgctggtcg gtgacatcta tttgctctgc tcggatgggt tgaatgacat ggtggaggat
     5401 gacgatatcc agtccacgtt gtatgccatg caagggaact tgccgttggc tgcggagcag
     5461 ttgattcaac tcgccaatga caatggtggt cgcgacaatg tctccgtcat cttggtcaag
     5521 gtgaaaggcg aattcgccgc atcgcgcaat tggctggaaa aattaatcaa ttggttttag
     5581 aaacaattaa aggattacga ctatggctgc aaaactgata ctcagtatgg atgggacggt
     5641 aatcaaggag tatcccttga gcaaggaacg tatgacgatc gggcggaagt cgcacaatga
     5701 tatcgtcatc gacaatctgg cgatcagtgg ggagcacgcc gccatcatca cgattctgca
     5761 tgactccttt cttgaagatc tcgacagtac caacggggtg gaggtgaatg gagtcgcggt
     5821 caagaagcat tttctgcaga acaacgatct gattgaaatt ggcaaataca agctgaagta
     5881 tattaacgat cagcttagcc aaaccaatgc tgccgacttt gaaaagacca tggtattgcg
     5941 tgcgccgatt aa
//
LOCUS       DASUIV010000141        14465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_499694, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000141 DASUIV010000000
VERSION     DASUIV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499694"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /gene="purT"
                     /locus_tag="VFW59_05235"
     CDS             <1..1163
                     /gene="purT"
                     /locus_tag="VFW59_05235"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEX5363649.1"
                     /translation="KVMLLGSGELGKEVLIALQRLGVETIAVDRYENAPGHQVAHRAH
                     VINMADGAALRALIEQEKPDLIVPEIEAIATDMLVQIEQEGLARVIPTARAAQLTMNR
                     EGIRRLAAETLGLPTSPYKFADSLEEMRAAAECVGYPCIVKPVMSSSGKGQSKVDGAD
                     EVQAAWDYAASGSRVNQGRVIVEGFIDFDYEITQLTVRAVGGNGAIETHFCEPIGHVQ
                     VHGDYVESWQPMAMSPLALQRARDIAKKVTDDLGGLGLFGVELFVKGDEVWFSEVSPR
                     PHDTGMVTMCSQRLNEFELHARAILGLPVDASMREPGASAVIYGQLEQKGIAYTGVDE
                     AMLVPHSEIRLFGKPESFVRRRMGVALATGRDTDEARARAKLAASKVVPVKG"
     gene            1160..1528
                     /locus_tag="VFW59_05240"
     CDS             1160..1528
                     /locus_tag="VFW59_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5363650.1"
                     /translation="MITGIHHAAFLTCDLGRARRFYEGVLGLHPDPNRPQMSFEGAWY
                     DIAANQQLHLMLLPDPEAGLSRPSHGGRDRHVALGVDDIDALRAKLDAAGIVYTPSQS
                     GRRALFCRDPDGNALEFIAS"
     gene            1621..1935
                     /locus_tag="VFW59_05245"
     CDS             1621..1935
                     /locus_tag="VFW59_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5363651.1"
                     /translation="MNAAEAVKALAALAQPTRLAIYRLLVVCGPEGMAAGQVAEKLKV
                     APATLSFHFKALSHAGLVESRQDGRFIYYAANFAAMNDMVAYLTENCCGGDASICQPK
                     KK"
     gene            1960..2460
                     /locus_tag="VFW59_05250"
     CDS             1960..2460
                     /locus_tag="VFW59_05250"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX5363652.1"
                     /translation="MLQDKTYNVLVLCTGNSARSILGEVLFNVLGKGKFRAYSAGSKP
                     AGKVNPGALEWLQAQGHSTEGLRSKSWDEFAAPGAPEFDFIFTVCDNAAGEACPLWLG
                     HPATAHWGIPDPAHVEGDEARRAAFKKAAEQLARRIQLFMSLPIEKLDKLTLKEKLAE
                     IGRIED"
     gene            2464..3489
                     /gene="arsB"
                     /locus_tag="VFW59_05255"
     CDS             2464..3489
                     /gene="arsB"
                     /locus_tag="VFW59_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX5363653.1"
                     /translation="MNLFERYLTLWVFLCIVAGVLLGQFLPGVFQAIAALEIASVNIP
                     VGVLVWVMIVPMLLRIDFGALHQVKAHVRGIGVTLFVNWAVKPFSMAFLGWLFIRHVF
                     ADYLPQDQLDSYVAGLILLAAAPCTAMVFVWSNLVKGDPYFTLSQVALNDTIMIFAFA
                     PLVGLLLGIASIVVPWATLFVSVVFYIVIPVVIAQLLRGRLLARGEGVFRRVMEYIAP
                     YSMGALLLTLVLLFALQGNAIVAQPLVIAMLAVPILIQVLFNSGLAYWLNRRLGVVHC
                     IAAPSALIAASNFFELAVATAISLFGFQSGAALATVVGVLIEVPVMLLVVKVANSSKG
                     WYARIEG"
     gene            3665..3868
                     /locus_tag="VFW59_05260"
     CDS             3665..3868
                     /locus_tag="VFW59_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5363654.1"
                     /translation="MSTGTVKWFNDAKGFGFITPDDGGEDLFAHFSAINMSGFKSLQE
                     GQKVSFEVTQGPKGKQASNIQAA"
     gene            4110..5270
                     /locus_tag="VFW59_05265"
     CDS             4110..5270
                     /locus_tag="VFW59_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5363655.1"
                     /translation="MALFVLLFSQLVIAGSPVYIGLDAEFGHKSSSSAQAIQQGIQIA
                     INEINQAGGVLGGRKLELVIKDNRSVTAIGVDNLRELAQMPDMVAVFGGKFSPIYIEC
                     VPVAHELGIPLLDPWGSADKITDHGLHPSYTFRLSLKDAWAGPAFVRFAKQQYKASRL
                     GVMLPNTSWGRSNQAAIEKAAAAAGVHLVGQRWYNWGDQSLRAQYDELRGAGAQAIIL
                     VANETEGSILVKEIAALPEAQRLPIISHWGVTGGDFADMTGDALQQVDFSVIQTFTFI
                     GLDTPVARRVLAAMKRDYGVGDASLIKSPVGVAHAYDLTHLLARAINKAGSTDRRKIR
                     DALEQLGPYDGLVRRYARPFSTDRHDALSAENIFFARYTADDKLVPLNRRQR"
     gene            5338..8367
                     /locus_tag="VFW59_05270"
     CDS             5338..8367
                     /locus_tag="VFW59_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5363656.1"
                     /translation="MAVRIKRAAFVMTMFIIVLLGSLMLVLTLLEIPGAELNANQGNV
                     NFIGEVLSVDFKNQLGSVERLSRSSLVWTALTDSAGREAYLRPFLQDQKDAGNIPMHL
                     LDYRGRPVLGEASAAIASGQLQALVGKVLADKHSRYAVIPNAGHPVLVGVFPVIFPYT
                     HDAIGALVGEVDVFGTFHARADGLGSDFGLDMLHQGHIIVNHAGAAPAIYFPAYFDLD
                     LGEAAEGGALTIKLYSTSNPWLAPVIKRILLSALLAVFLGVVVWRISGVVARRLSLRL
                     NALADACVAIAESRATTIPEDAGRDEIGVLSRTLHNAIESYEEINSHLEELVEEKTRK
                     LSESEARFRSFFENNSSVMLLIEPWSGKIIDANRAAASYYGYPRERMSGMHRREIDAA
                     AGMPVVGEAAPVSLEEKQHVISQHRLASGELRDVEVHSTPIASGDHVLLFVIVHDITE
                     RKQSERDIRIAAAAFETQEGILITDAAGLILRVNGAFSNITGYSMDEVVGKSPSILGS
                     GRQDHAFYEAMWASLNNTGTWAGEIWNRRKNGTIYPEYLTISSVKDGDGNVTNYVAAF
                     SDITVSKLAEDEIKSLAFFDPLTRLPNRRLLMDRLRHALSGSMRSKHQCALMFIDLDH
                     FKSLNDTLGHDIGDLLLQQVAQRLQDCVREGDTVARQGGDEFVVMLEELSSNTLEAAK
                     QAEMVGEKILAALNQPYQLAGHKHRNSPSIGITLFDGEQADTEELFKQADIAMYQSKK
                     AGRNTLSFFDPVMQATVTARVTLESDLRRAVIEQEEFQLYYQVQVDASGRRVGVEALI
                     RWNHPERGMVSPAEFIPLAEESGLILPLGHWVLATACKQLHNWAARPETAHLTMSVNI
                     SARQFRLPTFVEEVLALVDHFGLDPKRLKLEITESMLAHNVDEIIGKMIALKARGISF
                     SMDDFGTGYSSLQYLKRLPLAQLKIDQSFVRDIAVDESDRAIVRTIIAMAQSLDLDII
                     AEGVETEEQRQLLLEKGCNTFQGYLFGKPEPIDRFDV"
     gene            complement(8442..9230)
                     /gene="fabI"
                     /locus_tag="VFW59_05275"
     CDS             complement(8442..9230)
                     /gene="fabI"
                     /locus_tag="VFW59_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX5363657.1"
                     /translation="MGFLANKRILITGMISPRSIAYGVAQAMHREGAELAFTYQGERV
                     RDRVLNLAREFGSERVFQCDVGSDAEIARLFIDLNQHWDGLDGLVHAIAFAPREALDG
                     EFLDGFSREAFRIAHDISAYSFPALAKAALPMMQGRKAALLTLSYLGAVRTMPHYNVM
                     GMAKASLEASVRYLAMQLGREGIRANAISAGPIKTLAAAGIGDFGKLLSYNKKHSPLK
                     RNVTIEEVGNTAAFLCSDLASGITGEITYVDGGFNTTALGTPEA"
     gene            9352..9966
                     /gene="ccmA"
                     /locus_tag="VFW59_05280"
     CDS             9352..9966
                     /gene="ccmA"
                     /locus_tag="VFW59_05280"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEX5363658.1"
                     /translation="MLEVSNLACSRGDHRLFSGLSFTLHPGQIMQVQGENGSGKTSLL
                     RTLCGFLMPDEGDIVWNGGNIRELEEEYYAEMLYLGHLNAIKDELSALENLRISTGLA
                     GAELDEKDAVAALRRLGLKGRELLPTKVLSQGQRRRVALARLLVSDARLWVLDEPLAA
                     LDVTAVALIQEVIAEHLARQGMVIFTTHQPLLVEGMEMRSLRLS"
     gene            9996..10655
                     /gene="ccmB"
                     /locus_tag="VFW59_05285"
     CDS             9996..10655
                     /gene="ccmB"
                     /locus_tag="VFW59_05285"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5363659.1"
                     /translation="MLLLVIRRDLVLAMRRRADVLTTLIFFVMVVSLFPLGVGPEMDM
                     LRKMAPGVLWVAALLASMLSLGRLFSADYLDGTLEQMMLAPQSLSMLVLGKMVAHWMV
                     SGLPLVLMAPVLGVQFDMSAEALGVLILGLLLGTPVLSMMGAIGAALTLGLRGGGVLL
                     SLLVLPLCIPVLIFGTGAVEAVTTGLSVVSHLSMLGALLVLALAFTPWVTAQALRISM
                     E"
     gene            10661..11395
                     /gene="ccmC"
                     /locus_tag="VFW59_05290"
     CDS             10661..11395
                     /gene="ccmC"
                     /locus_tag="VFW59_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX5363660.1"
                     /translation="MAINWFKYSAPTTFYGLAGKLIPFFAIPAAVLFAIGLYIGFFIA
                     PTDYQQGEFYRIIFIHVPASILSMFLYLIMAAYAAMGLIFNTRLSAMMATAIAPTGAL
                     FALISLWTGALWGKPMWGAWWVWDARLTSELILLFLYLGYIALHASIDDPRRADRASA
                     LLAIVGSVNVPIIYFSVKWWNTLHQGSTIKFTGTSMHIAMQQAMFITLAACWLYAIAV
                     VLARVRSIILERERHTKWITELPEVK"
     gene            11395..11565
                     /gene="ccmD"
                     /locus_tag="VFW59_05295"
     CDS             11395..11565
                     /gene="ccmD"
                     /locus_tag="VFW59_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX5363661.1"
                     /translation="MNSLDIWMRENPITYVVLGSYAVAFLIFAVEIAMVRHKRKITLQ
                     QVRLMRDAGDEA"
     gene            11562..12035
                     /gene="ccmE"
                     /locus_tag="VFW59_05300"
     CDS             11562..12035
                     /gene="ccmE"
                     /locus_tag="VFW59_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5363662.1"
                     /translation="MKARHKRFMFIAIGIAAVGLAVALVLNALDKNVSLYFTPTQVFN
                     NEAPHGRSFRIGGLVEEGSVKREADGLTVNFDITDTHKKIPVVYKGILPDLFKEGKGV
                     VAQGKVGPDGVMHADEVLAKHDENYMPPEAADALKKAGEANAAAAASSAAASAVK"
     gene            12053..14038
                     /locus_tag="VFW59_05305"
     CDS             12053..14038
                     /locus_tag="VFW59_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX5363663.1"
                     /translation="MVPEIGHFSLIVAMFLAACMGVLPIIGAARNNAVLMGVARPLAV
                     GQFVFAGIALLCLAYAFVVNDFSVLYVAQHSNSQLPTVYRVSAIWGGHEGSLLLWAFL
                     LSVWTLAVAIYSKHLPEEMVARVLGVMGLVAVGFSLFMLLTSNPFDRLLPAAADGRDL
                     NPLLQDPGLVIHPPMLYMGYVGFSVAFAFAIAALLGGKLDATWARWSRPWTTVAWVFM
                     TLGIALGSWWAYYELGWGGWWFWDPVENASLMPWLLGTALIHSLAVTEKRGAFKSWTV
                     LLAIGAFTLSLLGTFLVRSGVLTSVHSFAADPKRGIFILSFLVVVIGASLLLFAWRAP
                     KIGLGGKFELLSRESMLLSNNVLLSVASASVFIGTLYPLFMDALGFGKLSVGAPYFEA
                     VFVPLMIPMVLIMGLGPLARWKKTSAPELGKRVRWAFASALVIALLLPLTMGKWSVMV
                     SVGLLLAFWILASLLVGLRERLAGRDDFMLRLRGQSLSYYGMQLAHLGVAVFIIGVTM
                     VKGYETERDVRMNVGDTLQAGGYEFRFNGVTEEQGPNYVAGRGSVTVTQDGKPVTELF
                     PEKRQYNVSGMPMTEAAIRPGLLLDLYVSLGEPIPDSDGAWAVRVYIKPFVDWIWAGC
                     LFMALGGVLAISDRRYRIHSKKTSQVAAELPLEEKTA"
     gene            14035..>14465
                     /locus_tag="VFW59_05310"
     CDS             14035..>14465
                     /locus_tag="VFW59_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX5363664.1"
                     /translation="MMRFILPFVLFVVLAGFLYVGLGLKPREVESPLIGKAAPAFSLP
                     QLHEPAKQFSPQEMKGKVWLLNVWASWCVSCREEHPVLVELSRQNIVPIYGLDYKDKN
                     ADAELWLKKGGDPYTLSVSDADGRVGIDYGVYGVPETYVID"
BASE COUNT         2636 a         3961 c         4649 g         3219 t
ORIGIN      
        1 ccaaagtgat gctgctcggc agcggcgagc tcggcaagga ggtgctcatc gccttgcagc
       61 gtctgggcgt ggaaaccatc gccgtggatc gctatgaaaa tgcaccgggc catcaggtcg
      121 cgcatcgcgc ccatgtcatc aacatggcgg acggcgcagc attgcgcgcg ctgatcgaac
      181 aggagaagcc cgacctgatc gtgccggaga tcgaggcgat cgccaccgac atgctggtgc
      241 agatcgagca ggaggggttg gcgcgtgtca tcccgaccgc gcgcgccgcg cagctgacga
      301 tgaaccgcga gggcatccgc cgtttggccg ccgaaacgct gggcctgccg acctcgccgt
      361 acaagtttgc cgacagcctg gaggagatgc gagccgcagc ggagtgcgtc ggttatccct
      421 gcatcgtcaa accggtgatg tcttcatccg gcaaggggca gtccaaggtg gacggcgcgg
      481 acgaggtgca ggccgcatgg gactacgccg cttccggttc gcgggtcaat cagggccggg
      541 tgatcgtcga gggctttatc gatttcgatt acgagatcac gcagctcacc gtgcgcgcgg
      601 tgggcggcaa cggtgcaatc gagacgcatt tttgcgagcc catcgggcat gtgcaggtgc
      661 atggagatta cgtcgagagc tggcagccga tggcgatgtc gccgctcgca ttgcagcgtg
      721 cgcgcgacat cgcgaagaag gtcaccgacg atctgggcgg gctgggtctg tttggcgtcg
      781 agctgttcgt caagggcgac gaggtgtggt tctccgaggt cagcccgcgc ccgcacgaca
      841 ccggcatggt gacgatgtgc agccagcgcc tcaacgaatt cgagctgcat gcccgcgcca
      901 tcctcggttt gccggtggat gcctcgatgc gcgagccggg tgcctcggcg gtgatctacg
      961 gtcagctgga gcagaagggc atcgcctaca ccggcgtcga cgaggccatg cttgtgccgc
     1021 actcggaaat ccgcctgttc ggcaagcccg agtctttcgt tcgccgccgc atgggcgtgg
     1081 cgctggcgac ggggcgcgac accgacgagg cgagggcgcg cgcgaagttg gcggcaagca
     1141 aggtggtgcc ggtaaaggga tgatcaccgg catccatcac gccgccttcc tgacctgcga
     1201 tctcggcagg gcgcgccgtt tctatgaagg cgtgctgggt ttgcatcccg atcccaatcg
     1261 tccgcagatg agcttcgagg gggcgtggta cgacatcgcg gctaaccagc agctgcacct
     1321 gatgttgttg cccgatccgg aagccggatt gtcgcgtccc tcccacggcg ggcgcgaccg
     1381 ccatgtggcg ttaggtgtcg acgatatcga tgcgttgcgg gcgaaactgg acgccgccgg
     1441 tatcgtttac acgccgagtc agtccggtcg ccgcgctctg ttttgccgcg atccggatgg
     1501 caatgcgctg gagttcatcg cgagttaaac cgcatccgtt tttatttgga aatgttttcc
     1561 ctgtctggta atctgcgtcc agacgtttta ataattgttt aacaatggaa atgtaaggtg
     1621 atgaacgcag ccgaagcagt gaaggcgctg gccgccttgg cgcaaccgac gcggctggcg
     1681 atctatcgcc tgctggtggt gtgcgggccg gaagggatgg cggcggggca ggtggccgaa
     1741 aagctgaagg tggctccggc cacgctgtca ttccatttca aggcgctcag tcacgcgggg
     1801 ctggtggaga gccggcagga tgggcgcttc atttattacg cggcgaattt tgcggcgatg
     1861 aacgacatgg tcgcctatct gacggagaac tgctgcggcg gtgatgccag catctgccaa
     1921 ccgaagaaga aatgatcgaa accagtattc aaggagagta ttttgcaaga caagacctat
     1981 aacgtgctgg tgctatgtac cggcaattcc gcgcgcagca tcctgggcga agtgctgttc
     2041 aatgtgctgg gaaaaggcaa gttcagggcc tatagcgcag gcagcaagcc ggcggggaaa
     2101 gtgaatccgg gcgcactgga atggttgcag gcgcaggggc acagcacaga aggcctgcgc
     2161 agcaaaagct gggacgagtt cgccgctccc ggcgcgccgg aattcgattt cattttcacc
     2221 gtatgcgaca acgccgccgg agaagcctgc ccgctgtggc tgggacatcc ggctacggcc
     2281 cattggggga tacccgatcc ggcccacgtc gagggcgatg aagcgcggcg tgcggcgttc
     2341 aagaaggctg ccgagcaatt ggcgcgccgc atccagttgt tcatgagtct gccgatcgaa
     2401 aagctggaca aactcacgct gaaggaaaaa ctcgccgaga tcgggcggat cgaggactaa
     2461 gccatgaacc tgttcgagcg ctatctgact ttgtgggttt ttctgtgcat cgttgccggc
     2521 gtgctgctcg ggcagtttct gccgggggtg ttccaggcta tcgccgcact ggaaatcgcc
     2581 agcgtgaata tcccggtggg ggtgctggtg tgggtgatga ttgtgccgat gttgctgcgg
     2641 atcgacttcg gcgcgctgca tcaggtgaag gcgcatgtgc gcggcatcgg cgtcacgctg
     2701 ttcgtcaact gggcggtgaa gccgttctcg atggccttcc tgggctggct gttcatccgc
     2761 catgtgtttg ccgattacct gccgcaggat cagctcgaca gctatgtcgc aggattgatc
     2821 ctgctggccg cagcaccctg caccgcgatg gtgttcgtgt ggagcaacct ggtgaagggc
     2881 gatccgtatt tcacgctgtc gcaggtcgcg ctcaacgaca ccatcatgat cttcgccttc
     2941 gcgcccttgg tcgggctgct gctcggcatt gccagcatcg tcgtgccttg ggcgacgctg
     3001 ttcgtctcgg tggtgttcta tatcgtgatc cccgtggtca tcgcgcaact gttgcgtgga
     3061 cgtttgctgg caagaggcga gggcgtcttc aggcgagtca tggagtatat cgccccgtat
     3121 tcgatgggcg cgctgctgct gacgctggtg ttgctgtttg cgctgcaggg caacgcgatc
     3181 gtcgcgcagc cgctggtgat cgcgatgctg gctgtgccta tcctgatcca ggtgctgttc
     3241 aactccggtc tggcctattg gctgaaccgc cgcctcggcg tggttcactg cattgccgcg
     3301 ccgtcggcgt tgatcgctgc gagcaacttc ttcgagctgg ctgtcgcaac cgcgatcagc
     3361 ctgttcggtt tccagtcggg tgccgctctg gcaacggtgg tgggggtgtt gatcgaggtt
     3421 ccggtgatgc tgctggtggt gaaagtcgcc aattcgagca agggttggta cgctcgaatc
     3481 gagggctgaa accatctttt cgcatgattt atgataaatc ccttgacgct gtgggattaa
     3541 ccacgtaaaa cgcacgcttg tgtttgattc aaagtctgtg ctgtactgct tgtcatgtgc
     3601 aatcttcgat tcaaatagtc ccgcgggggg gatttccccg tttttctagt aaggaagtaa
     3661 caacatgtca actggtacag ttaagtggtt caatgatgca aaaggtttcg gtttcatcac
     3721 tcctgatgat ggtggcgaag atctgttcgc gcacttctcg gcgatcaaca tgagcggctt
     3781 caagtcgctg caagaaggcc aaaaagtcag cttcgaagtg actcaaggtc ctaaaggcaa
     3841 gcaagcctcg aacatccagg ctgcctaatc gctccctgaa ttgatcgaaa aaagccacct
     3901 tcgggtggct ttttttcgcc tgtcatttga ctcttcatgg gaaccccctg tctgttccag
     3961 gcgatgtggc atcttcatgc ggctctcctg cccggctcaa gtcatgcggc gtcagataat
     4021 tctgacatgt ctgaacccgg gtgaaatata attcgcaggt tcttcatatc gggtttaagg
     4081 taatgaaatc cacaattatt caatggttaa tggcgctttt tgttttgctg ttcagccaac
     4141 ttgtcatcgc gggcagtccg gtatacattg gcctggatgc agaattcggg cataagtcca
     4201 gctcctcggc acaggcgata cagcagggga ttcagattgc gatcaacgag atcaatcagg
     4261 ccggaggggt gctgggtggc cgcaagctcg agctggtgat caaagacaac cgctcggtca
     4321 cggccatcgg tgtcgacaac ttgcgcgaac tggcccagat gccggatatg gtcgccgtgt
     4381 ttggtggcaa gttcagtccg atctatatcg agtgtgtgcc cgtggcccat gaattgggca
     4441 ttcccttgct tgacccctgg ggctcggcag acaagattac cgaccatgga ctgcatccca
     4501 gttatacgtt ccgcctgtca ttgaaagatg cctgggcggg gccggccttc gttcgttttg
     4561 ccaagcaaca gtacaaggcc agtcgtctcg gcgtcatgct tcccaatacc tcatgggggc
     4621 gcagcaatca ggctgccatc gagaaggccg ctgccgccgc aggtgtccat ctggtcggac
     4681 aacgctggta caactggggc gatcagtcgc tgagggcgca atacgatgaa ttgcgcggtg
     4741 cgggagcgca agccatcatc ctcgtcgcca atgaaacgga ggggtcgata ctggtcaagg
     4801 agatcgcagc gctgcccgag gcgcaacggc tgcccatcat cagccattgg ggcgtcacgg
     4861 gaggcgactt cgccgacatg accggcgatg ccctgcagca ggtcgatttt tccgtgatcc
     4921 agacttttac ttttatcggg ctggatacac ccgttgcacg cagggtgctg gccgcgatga
     4981 agcgtgatta cggtgtcggg gatgcgagcc tgatcaagag ccccgtgggt gtcgctcatg
     5041 cctacgacct gacccatctg cttgcacggg cgatcaacaa ggctggcagc accgacaggc
     5101 gcaaaatccg ggatgcattg gagcaactcg gcccctacga tggtctggtg cggcgttacg
     5161 cacggccctt cagtaccgac cgccacgatg ccttgtctgc cgagaatatt ttctttgccc
     5221 gttatacggc agatgacaaa ttggttccgc tcaatcggcg tcaacgttga gcacttgcca
     5281 gtgttgcggg tataagcaag gatgattttc aagacgctca gtggcaacga tctgcctttg
     5341 gctgttcgca tcaagcgagc cgccttcgtc atgacgatgt ttatcatcgt cctgctcggt
     5401 agcctgatgc tggttttgac gctgctcgag attccggggg cggagttgaa tgccaatcag
     5461 ggcaacgtca attttatcgg cgaagtgctg tccgtcgatt tcaagaacca gctgggctcg
     5521 gtcgagcgac tgagccgcag ttctctggtc tggaccgcgc tgacggactc cgccggccgc
     5581 gaggcctatt tgaggccgtt tctgcaggat cagaaggatg caggcaacat tcctatgcac
     5641 ctgctcgatt atcgtgggcg gcctgttttg ggagaggcct ctgcggcgat agcgtccggt
     5701 cagcttcagg cgttagtcgg caaggtgctg gccgataaac attcgagata cgcggtgatc
     5761 cctaacgcgg gacaccccgt tctggtcggc gtgttcccgg tgatattccc ctacactcat
     5821 gatgctatcg gggcattggt cggtgaggtc gatgttttcg ggacgttcca cgccagggcg
     5881 gacggactcg gttcggattt cggtctggat atgcttcatc agggccacat catcgtcaac
     5941 catgctggtg cggcccctgc gatttatttc ccggcttatt tcgacctcga tctcggtgaa
     6001 gccgccgagg gcggtgcgct gacgatcaag ctatattcaa cttccaatcc atggctggct
     6061 cctgtcatca agcgcattct gctgtccgca ctgctcgccg tgttcctcgg tgtggtggtc
     6121 tggcggatat cgggggtcgt cgcgcgacgt ctttctctgc ggctgaatgc cctggccgat
     6181 gcctgtgtcg ccattgccga atcgcgcgcc acgaccattc ctgaagatgc gggcagggat
     6241 gagatcggtg tcttgtcgcg caccttgcac aatgccattg aatcgtacga ggaaatcaat
     6301 tcccatctgg aagagctggt tgaggaaaaa acgcgaaaac tgtcggagag tgaagcgcgt
     6361 ttccgcagct tctttgaaaa caattcttcg gtgatgttgc tgatcgagcc ttggtcgggc
     6421 aagatcatcg atgccaacag ggctgctgca agttattacg gctatccccg ggagaggatg
     6481 agcggcatgc atcgccgcga aatcgatgcg gcagcgggca tgcctgtcgt cggagaggcg
     6541 gcaccggttt cgctcgagga aaaacaacat gtcatctcgc aacatcgcct ggccagcggt
     6601 gagttgcgcg atgtggaggt gcattcgaca ccgatagcga gcggagatca cgtcctgctg
     6661 tttgtcattg ttcacgacat caccgagcgc aaacagagcg aacgcgatat tcgcatcgcc
     6721 gccgccgcct tcgagacgca ggagggcatc ctgatcaccg acgccgcagg cttgatactc
     6781 agggtgaacg gagccttcag caatatcacc ggctacagca tggatgaggt ggtcggcaag
     6841 agtccgagca tactcggctc ggggcgtcag gatcacgcat tctatgaggc catgtgggcg
     6901 agtctgaata acaccggtac atgggcaggc gaaatctgga accgccgcaa gaatggcacc
     6961 atctatccgg aatatctcac gatctcctcg gtaaaggatg gtgatggaaa cgtcaccaat
     7021 tatgtggctg ccttcagtga tattaccgtg agcaagctgg cggaggatga gatcaagagc
     7081 ctggccttct tcgatccgct gacccgcttg cctaacaggc gattgctgat ggaccggctc
     7141 aggcatgcgc tgtcgggcag catgcgcagc aagcatcaat gtgcgctgat gtttatcgat
     7201 ctggatcatt tcaagtctct taacgatacg ctgggtcacg acatcggcga tctgctgctg
     7261 caacaggtcg cgcagcgtct gcaagactgc gtccgcgagg gagataccgt ggctcgtcag
     7321 gggggggacg agttcgtggt gatgctggaa gagctgagca gcaatacgct ggaagccgct
     7381 aagcaggcgg agatggtcgg cgagaaaatc cttgccgcgc ttaaccagcc ataccagctt
     7441 gccggacaca aacaccgcaa ctccccgagc atcggcatta ccttgttcga cggagaacag
     7501 gctgatacgg aggagctgtt caagcaggcc gacatcgcga tgtaccagtc caagaaggcg
     7561 gggcgcaata ccctgagttt cttcgatcct gtcatgcagg ccacggtgac ggctcgcgtc
     7621 acgctggagt ccgatctgcg ccgggccgtg atcgaacagg aggagtttca gctttactat
     7681 caggtgcagg tcgatgcgtc gggtcgtcgg gtcggcgttg aggcgctgat tcgctggaac
     7741 catcccgagc gcggcatggt ctcgcctgcc gaattcatcc cgcttgccga agagtctggc
     7801 ctgatcctgc cattggggca ctgggtgctg gcaaccgcat gtaaacagtt gcataactgg
     7861 gcggccagac ccgaaacggc ccatcttacg atgtcggtga atatcagcgc acgccagttc
     7921 cgtctgccga ccttcgtcga ggaggtgctc gcgttggtcg atcacttcgg gctggatccg
     7981 aaaagactca agctggagat caccgaaagc atgttggccc acaacgtgga cgagatcata
     8041 ggcaagatga tcgcgctgaa ggccagaggc attagttttt cgatggacga tttcggtacc
     8101 ggctattcct cgctgcaata cctcaagcgc ctcccgctcg cccagctcaa gatcgaccag
     8161 tcatttgtgc gcgatatcgc cgttgatgaa agcgacaggg cgattgtgcg taccatcatt
     8221 gccatggcgc agagcctgga tctggatatc attgccgaag gggtggaaac ggaggagcag
     8281 cggcaactct tgctggagaa ggggtgcaac acttttcagg gctatctatt tggcaagcct
     8341 gagccgattg atcgattcga tgtctgattc gacagcgggt cttcgcaaag cgcttccttc
     8401 agaacgccac ccctcgggtg gcgtttttga tgttgcaccg ttcaggcttc cggggtgccc
     8461 agtgccgttg tgttgaagcc gccgtccaca taggtgattt cgccggtgat gccgctggca
     8521 aggtcgctgc acaggaaggc tgcggtgttg ccgacttctt cgatggtgac gttgcgtttg
     8581 agcggggaat gctttttgtt gtagctcagc agtttgccga agtcgccgat accggctgca
     8641 gccagtgtct tgatcgggcc tgcggagatg gcgttcgcgc gtatgccttc gcggcccagt
     8701 tgcatcgcca gataacgcac gctggcttcc aggctggcct tggccatccc catcacgttg
     8761 taatgcggca tggtgcgtac cgcaccgaga tagctcaggg tcagcaacgc ggccttgcgg
     8821 ccttgcatca tcggcagcgc ggcctttgcc agcgccggaa aactgtaggc gctgatgtcg
     8881 tgtgcgatgc ggaatgcttc gcggctgaaa ccatccagaa actcgccgtc cagtgcttcg
     8941 cgaggcgcga aggcgatggc gtgtaccaat ccgtccagcc cgtcccagtg ttggttcaga
     9001 tcgatgaaca gccgggcgat ctcggcatcg ctgccaacgt cgcattgaaa cacccgctcg
     9061 ctgccgaatt cccgggccag attcaacacg cggtcgcgca cccgttcgcc ctgataggtg
     9121 aaggccagtt ccgctccctc gcggtgcatc gcctgggcga cgccgtaagc gatggaacgg
     9181 gggctgatca tgccggtaat caggatgcgt ttgttggcga gaaatcccat gctgttcctc
     9241 tgcgcttgac gagtaaaaac ccttctgatt atagcggtaa atgaatggtg cgcatgcgcc
     9301 ggatgcaatc gtatataatc ctgcgctgtt caaatatcag gttttctcta catgctggaa
     9361 gtcagcaatc ttgcttgcag tcgcggtgac catcgcctgt tttccggctt gagtttcacg
     9421 ctgcatcccg ggcaaatcat gcaggtgcag ggcgagaacg gcagcggcaa gaccagtctg
     9481 ctgcgtaccc tgtgcggctt cctgatgccg gatgagggcg atatcgtctg gaacggcgga
     9541 aatatccgcg agctggagga agagtattac gccgagatgc tgtacctcgg gcacctgaat
     9601 gcgatcaagg atgagttgag cgcgctggag aatctgcgca tctcgaccgg ccttgcgggt
     9661 gccgaactgg atgagaagga tgcggtcgcc gccttgcgtc gtctggggtt gaaggggcgc
     9721 gagctgctgc cgaccaaggt gctgtcgcaa gggcagcggc ggcgcgtggc gttagccaga
     9781 ctgctggtga gcgatgcaag gttatgggtg ctggatgaac ctttggcagc actcgatgtc
     9841 acagcggtag cgctgattca ggaagtgatc gccgaacatc tggcgcgtca ggggatggtt
     9901 atctttacca ctcatcaacc gttgctagtg gagggaatgg aaatgcgttc cctgagattg
     9961 tcatgagtgc ggtaaagaaa gcttcgatgc tcgacatgct gctgctggtg atacggcgcg
    10021 acctggtgct ggcgatgcgt cgccgcgcgg acgtgctgac tactctgatc ttcttcgtga
    10081 tggtggtcag cctgttcccg ctgggggtgg gtcccgagat ggacatgctg cgcaagatgg
    10141 cgccgggcgt gttgtgggtc gcggcgttgc tggcctcgat gctgtcgctg ggcaggttgt
    10201 tctcggcgga ttatctggac ggcacgctgg agcagatgat gctggcgccg cagtccttgt
    10261 cgatgctggt gctgggcaag atggtggcgc actggatggt atccggtctg ccgctggtgt
    10321 tgatggcgcc ggtgctgggc gttcagttcg acatgtcggc cgaggcgctg ggcgtgttga
    10381 tactcggcct gttgctgggc acaccggtgc tgagcatgat gggggcgatc ggtgcggcgt
    10441 tgacgctggg cttgcgcggt ggtggtgtgt tgttgtcgtt gctggtgctg ccgttgtgta
    10501 ttcccgtgct gatcttcggt acgggggcgg tcgaggcggt gacgaccgga ttgagcgtgg
    10561 tgtcccatct gtccatgttg ggtgcattgt tggtgttggc tctggcgttt accccctggg
    10621 tgaccgcgca ggcgttacga atttcgatgg agtgattaag ttggcaatta attggtttaa
    10681 gtattccgcc cccaccactt tctacggcct ggccgggaaa ttgattccgt tcttcgcgat
    10741 ccccgctgcg gtgctgttcg ccatcggttt gtatatcggc ttcttcatcg cgccgaccga
    10801 ttatcagcag ggcgagtttt accgcatcat cttcatccat gtgccggcct ccatcctgtc
    10861 gatgttcctg tatctgatca tggcggccta tgccgcaatg gggctgatct tcaacaccag
    10921 gctgtcggcg atgatggcga cggccatcgc accgaccggc gcgctgttcg cgctgatctc
    10981 gctgtggacc ggcgcgctgt ggggcaagcc gatgtggggt gcatggtggg tgtgggatgc
    11041 gcgcctgacc tcagaactga tcctgctgtt cctgtatctc ggctatatcg cgctgcacgc
    11101 ttcgatcgac gatccgcgtc gcgccgaccg cgccagcgca ttgctcgcca tcgtaggttc
    11161 ggtgaacgtg cccatcatct atttctcggt gaagtggtgg aacaccctgc atcagggttc
    11221 gaccatcaag ttcaccggaa ccagcatgca tatcgcgatg cagcaggcga tgttcatcac
    11281 gctggcagcc tgctggctgt atgccatcgc cgtggtgctg gcgcgggtgc gcagcatcat
    11341 cctggaacgc gaacgccata ccaagtggat cactgaattg ccggaggtca aataatgaac
    11401 agtctggata tctggatgcg tgagaacccg atcacctacg tggtgctggg ttcctacgcg
    11461 gtcgcctttc tgatttttgc ggtcgagatc gccatggtca ggcataaacg aaaaatcact
    11521 ttgcagcagg tacgcctgat gcgtgacgca ggggacgaag catgaaggca cgccacaaaa
    11581 gattcatgtt catcgccatc gggatcgccg ccgtcgggct ggcggtcgct ctggtgctga
    11641 atgcgctcga caagaatgtc agcctgtatt tcaccccgac ccaggtgttc aacaacgagg
    11701 ctcctcatgg ccgcagtttc cgtatcggcg gactggtcga ggagggcagt gtgaagcgcg
    11761 aggccgacgg tctgacggtg aacttcgaca tcaccgacac gcacaagaaa atcccggtgg
    11821 tatacaaggg catcctgccc gacctgttca aggaaggtaa gggcgtggtc gcgcagggca
    11881 aggtcgggcc ggacggcgtg atgcacgccg acgaggtgct ggccaagcac gatgaaaact
    11941 acatgccccc cgaagctgcc gacgcactga aaaaagccgg agaggcgaac gccgctgcgg
    12001 ccgcttccag cgcagccgct tccgccgtca aataattcct gaaagttaaa atatggttcc
    12061 agagataggt catttttcgc tgatcgtggc gatgttcctg gccgcgtgca tgggcgtgct
    12121 gcccatcatc ggcgcggcgc gcaacaatgc ggtgttgatg ggggttgcgc gtccgctggc
    12181 ggttgggcag tttgtgttcg ccggtatcgc cttgttgtgt ctggcttacg ccttcgtcgt
    12241 caacgatttc tccgtgttgt atgtcgccca gcattccaat tcgcaactgc cgaccgtgta
    12301 tcgggtttct gcgatctggg gcgggcacga ggggtcgctg ctgctgtggg ccttcctgct
    12361 gtcggtgtgg acgctggccg tcgccattta cagcaaacat ctgccggaag agatggtggc
    12421 gcgggtgctg ggtgtgatgg ggctggtcgc ggtcggcttc tcgctgttca tgctgctgac
    12481 ctcaaatcct ttcgatcgtc tgctgcccgc tgctgccgat gggcgcgacc tgaatccgct
    12541 gctgcaggat cccggtctgg tgatccatcc gccgatgctg tacatgggct atgtcggatt
    12601 ctcggtggcg ttcgccttcg cgatcgcagc cctcttgggt ggcaagctgg atgcgacctg
    12661 ggcgcgctgg tcgcgtccct ggaccaccgt ggcctgggtg ttcatgacgc tgggcatcgc
    12721 gctgggcagc tggtgggcct attacgaatt gggctggggc ggctggtggt tctgggatcc
    12781 ggttgagaac gcatccttga tgccctggtt gctgggcacg gcgctgatcc attcgctggc
    12841 cgtaaccgag aagcgcggcg cgttcaagag ctggacggtg ctgctggcga tcggcgcgtt
    12901 cacgctgagc ctgctgggaa ccttcctggt gcgttccggc gtgctgacct cggtgcactc
    12961 cttcgcggcc gatccgaagc gcggcatctt catcctgtcc ttcctggtcg tcgtgatcgg
    13021 tgcgtcgctg ctgctgttcg cctggcgtgc gccgaagatc ggtctgggcg gcaagttcga
    13081 actgctgtcg cgcgaatcga tgctgctgtc gaacaacgtg ctgctttccg tcgcttccgc
    13141 ctcggtgttc atcggcacgc tctacccgct gttcatggat gcgctcggtt tcggcaagct
    13201 gtcggtcggc gcgccgtatt tcgaggcggt gttcgtgccc ttgatgattc cgatggtgct
    13261 catcatgggg ctgggcccgc tggcccgctg gaagaagacc agcgctccgg aactcggtaa
    13321 gcgtgtgcgc tgggcattcg ccagtgcgct ggtcattgcc ttgctgttgc cgctgaccat
    13381 gggcaagtgg agcgtgatgg tttcggtcgg tttgttgctg gcgttctgga tactcgcttc
    13441 tttgctggtg gggctgcgcg agcgtctggc cggacgcgac gacttcatgc tgcgtctgcg
    13501 cggccaatcc ttgagctatt acggcatgca gctcgctcac ctcggcgtgg cggtgttcat
    13561 catcggcgtg accatggtca agggctatga gaccgagcgc gatgtgcgca tgaatgtcgg
    13621 cgatacgctg caggcgggcg gttatgaatt ccgcttcaac ggcgtcaccg aagagcaggg
    13681 gcctaactat gtggccggtc gcggcagtgt gacggtcact caggacggca agccggtcac
    13741 cgagctgttc ccggaaaagc gtcagtacaa cgtctccggc atgccgatga ccgaggcggc
    13801 gatccgtccc ggcctgctgc tcgacctgta tgtgtcgctg ggtgaaccga tccccgacag
    13861 cgatggagcc tgggcggtgc gcgtctacat caagccgttc gtggactgga tatgggcagg
    13921 ttgtctgttc atggcgctgg gcggcgtact ggcgatcagc gatcgtcgct atcgcattca
    13981 cagcaagaaa accagtcagg tggctgcaga gttgcccctg gaggagaaga ccgcatgatg
    14041 cgtttcatat tgccgtttgt gctatttgtg gtgctggcgg gcttcctgta tgtcggactc
    14101 ggcctcaaac cccgcgaagt ggaatcgccg ttgatcggca aagcggctcc ggccttctcg
    14161 ttgccgcagc ttcatgaacc ggccaagcaa ttctctccgc aggagatgaa gggcaaggtc
    14221 tggctgctga acgtgtgggc gtcctggtgc gtgtcgtgcc gggaagagca tccggtgttg
    14281 gtggagctgt cgcgccagaa catcgtgccg atctacggtc tggactacaa agacaagaac
    14341 gcggatgccg aactgtggct gaagaagggt ggagatccct atacgctcag cgtctccgat
    14401 gcggatggac gtgtcggcat cgactacggt gtgtacggcg tgcccgagac ctatgtgatc
    14461 gacaa
//
LOCUS       DASUIV010000142         6123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_869641, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000142 DASUIV010000000
VERSION     DASUIV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869641"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VFW59_05315"
     CDS             complement(<1..1472)
                     /locus_tag="VFW59_05315"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5363665.1"
                     /translation="MELAKSFDPKDIEARWYPQWENAGYFKAGLDPNKQENFCIQLPP
                     PNVTGTLHMGHGFNQTLMDALTRYHRMKGDNTLWQPGTDHAGIATQIVVERQLDAQGV
                     SRHDLGREKFIEKVWEWKEYSGNTITKQMRRLGTSPDWSRERFTMDPKLNKTVTETFV
                     RLFNEGLIYRGKRLVNWDPKLHTAVSDLEVVQEEEDGFMYHIQYPLADGEGFLVVATT
                     RPETLLGDVAVMVHPEDERYRHLIGKQVKLPLCDRTIPVIADEYVDKEFGTGVVKVTP
                     AHDFNDYAVGQRHALPMISILTLDAKINEHAPAKYQGLDRFDARKVIVSDLESAGLFV
                     KADKHKLKVPRGDRTNVVIEPMLTDQWFVAMSKAGPEGKSITEQALECVASGEIKFHP
                     ENWVNTYNQWLNNIQDWCISRQLWWGHQIPAWYADDGRVFVAHDEAEAKAVAAAEGYT
                     GALKRDDDVLDTWYSSALWPFSTLDWTPEWPAQSNDALDL"
     gene            complement(1593..1931)
                     /locus_tag="VFW59_05320"
     CDS             complement(1593..1931)
                     /locus_tag="VFW59_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363666.1"
                     /translation="MSPADDITSDLPDNLPLLTQVVGEPAPDELPTLTDIVAPAVPAP
                     AAKTLSDEEMQQLLRLLESRLEVVFSQKLSLHLEKLQRLAVKQAVNEFRAALPEMLRD
                     ILNTPPDSRR"
     gene            complement(1928..2359)
                     /locus_tag="VFW59_05325"
     CDS             complement(1928..2359)
                     /locus_tag="VFW59_05325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX5363667.1"
                     /translation="MTKVDFYTGSTDKLRTACQLSHKAMQNGVRVVLSTPDAASSEEL
                     DKLLWQYPATGFIPHCRSDAEDAETMPVVLNNDSENFPHHDLLISLHNECVPFFSRFE
                     RVIEIVGKDDEDSRMGRERYKFYKDRGYELSHIDLSKAKNA"
     gene            complement(2359..3846)
                     /locus_tag="VFW59_05330"
     CDS             complement(2359..3846)
                     /locus_tag="VFW59_05330"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX5363668.1"
                     /translation="MEFSIKQGSPEKLRSGCVVVGVYDGGKLSAAAQDLDKAAAHHLS
                     DVIARGDMTGKAGNTLMLHHVAGITAERVLLVGLGKAAEFADKAYLDALRAAFAALQK
                     TASKDAALFLTDLDVKGRDANWRIKQAVLAAAESDFRSDQLKSKPADASTLHKIVLGC
                     AAKPDSAAQRALDQAVATAHGMELTKTLGNLPGNICTPSYLAAKALALGKAHKNIKTT
                     VLEEKDMQKLGMGSFLSVTRGSEQPAKLITMEYHGADKKQKPIVLVGKGVTFDSGGIS
                     LKPGAEMDEMKYDMCGAASVLGTLQAIAEMGLKLNVVGVIPACENLPSGRATKPGDIV
                     TSMSGQTIEILNTDAEGRLILCDALTYSAKFKPDTVIDIATLTGACVIALGNVASGMF
                     SNQDDLAKELLEASAQSNDRIWQLPLWDEYQPLLDSNFADMQNIGGRAGGTITAACFL
                     SRFTKDYRWAHLDIAGTANKSGKEKGATGRPVPLLTQYLINRAQK"
     gene            3931..5043
                     /gene="lptF"
                     /locus_tag="VFW59_05335"
     CDS             3931..5043
                     /gene="lptF"
                     /locus_tag="VFW59_05335"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX5363669.1"
                     /translation="MGVFQRALIGELTSNGLLVFAVLLGIVVVSQLIRLLGDAVSGRI
                     AVDGVLALLGFAAMNYLPVLLSISLFISILLTISRSYRDSEMVVWFCAGIGLTRWIRP
                     VMLYTLPIVGLIALLSLLLSPWALQKAEEFKRKLESRDDVAAAIPGTFRESGQADRVY
                     FIEKVDIGSNRVGNVFVQSLQNGKQGTMVASQGLQETSPNGDRFLVLLNGTRYEGTPG
                     KLDYNIVDFERYSIRIDSSPPKQANTWVRSMSTPELLQDESLWARAELDWRLGLPISA
                     AILALLAIPLSYVNPRAGRSLNMVLAIVLYMLYSNMISVSNSWVAQGKLSHLIGLWAV
                     HLIMLAITALMFQRRMSLLSWRRLFGKRGSMRPGRT"
     gene            5040..6113
                     /gene="lptG"
                     /locus_tag="VFW59_05340"
     CDS             5040..6113
                     /gene="lptG"
                     /locus_tag="VFW59_05340"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX5363670.1"
                     /translation="MNLLTRYLGREIYLSIALVFSALIALFAFLDMVRELSVMQGQYN
                     LSYVLLFVVLTIPGHIYELFPVAVLVGTIFAMVQMAASSELTVYRASGASLWQMIGAL
                     FKIALPLVALSFVFSEFVAPPSERLAQELRQKAQNAQVVFAEFRSGTWVKDERSFVNV
                     KTVMPDGSLTEVDIYRFDETNHLQAITHASQARFVEQGRWQLAGVRETRFDKQGISVR
                     AVDEQEWPSALNPGILSVLLVVPEQMSAYDLYQYTVHLKENRQQSARYDIAMWGKLVY
                     PFALLVMMILALPFASYRRRSGGVSAMVFMGIVLGLVFHFVGRLFASLGALNDWQPFI
                     SAAAMSVLFLFLGLAMLWWTERR"
BASE COUNT         1052 a         1663 c         1958 g         1450 t
ORIGIN      
        1 tagaggtcca gcgcgtcgtt ggactgggcc ggccactcgg gcgtccagtc cagggtgctg
       61 aagggccaca gggcggacga gtaccaggtg tcgagcacgt cgtcgtcacg cttcagggcg
      121 ccggtgtagc cctcggccgc agcgacggct ttggcttctg cctcgtcgtg ggcgacgaag
      181 acgcggccgt cgtcggcata ccacgccggg atctggtggc cccaccaaag ttggcgcgag
      241 atgcaccagt cctggatgtt gttcagccac tggttgtagg tgttcaccca gttctcgggg
      301 tggaacttga tctcgcccga ggccacgcat tccagcgcct gttcggtgat gctcttgcct
      361 tcagggcccg ccttgctcat cgccacgaac cactggtcgg tgagcatcgg ctcgatgacc
      421 acattggtgc ggtcgccgcg cggcactttg agtttgtgct tgtcggcctt gacgaacagc
      481 ccggcggatt cgagatcact cacaatcacc ttgcgcgcgt cgaagcggtc gaggccttgg
      541 tatttggcgg gtgcgtgttc gttgatcttc gcgtccagcg tgaggatgga aatcattgga
      601 agcgcatggc gctgccccac cgcatagtcg ttgaagtcgt gcgcaggcgt gactttcacc
      661 acgccggtgc cgaattcctt gtccacgtat tcgtcggcga tgaccgggat ggtgcggtcg
      721 cacagcggca gtttcacctg cttgccgatc aaatgccggt agcgctcgtc ttcgggatgc
      781 accatcaccg ccacgtcgcc cagcagggtc tcggggcgcg tcgtcgcgac caccaggaaa
      841 ccttcgccat ccgccagcgg atactggatg tgatacatga agccgtcttc ctcttcctgc
      901 accacttcga ggtcgcttac tgcggtgtgc agtttcgggt cccagttcac cagacgcttg
      961 ccgcgataga tcaggccttc gttgaacagg cgcacaaagg tctcggtgac ggtcttgttc
     1021 agtttcggat ccatcgtgaa gcgctcgcgc gaccagtcgg gcgaagtgcc caggcggcgc
     1081 atctgcttgg tgatggtgtt gccggagtat tccttccact cccagacttt ttcaatgaac
     1141 ttctcgcgac ccaggtcatg gcgcgagacg ccctgtgcgt cgagctggcg ctcgacgacg
     1201 atctgcgtgg cgatacccgc atggtcggtg cccggctgcc acagcgtgtt gtcgcctttc
     1261 atgcggtggt agcgggtgag cgcatccatc agcgtctggt tgaagccgtg ccccatgtgc
     1321 aaggtgccgg tgacgttggg cggcggcagc tggatgcaga agttctcctg cttgttcgga
     1381 tcgagtccgg ccttgaagta accggcattc tcccactggg ggtaccaacg ggcttcgatg
     1441 tctttcgggt caaaactttt tgcgagttcc atactggggt tccattgcgg cgctggcggg
     1501 cttgttctgg acatgccgtt cagccggttc gaagagcgcg cgattatagc aaagaggagg
     1561 gagctaagcc tcccttgtgc gtcgccgtgc cgttaacggc gggagtcggg cggtgtattc
     1621 aggatgtcgc gcagcatttc cggcagggcg gcgcgaaatt cgttgacggc ctgcttcacg
     1681 gccagtcgct gcaatttctc gagatgcagg ctgagtttct gcgagaacac gacttcgagg
     1741 cgggattcga gcagtctcag cagttgctgc atttcctcat cgctgagcgt ttttgcggcg
     1801 ggagcgggaa cggcgggagc gacgatatcg gtgagcgtgg gcagttcatc cggtgcgggc
     1861 tcgccgacca cctgagtcag cagcggcaga ttgtccggca agtcggaggt gatgtcgtcg
     1921 gcggggctca tgcgtttttc gccttggaaa ggtcgatatg gcttaactcg tagccgcgat
     1981 ccttgtagaa cttgtaacgc tcgcgtccca tgcggctgtc ttcgtcatcc ttgccgacga
     2041 tttcgatgac gcgctcgaag cggctgaaaa atggcacgca ttcgttatgc aggctgatca
     2101 gcagatcgtg gtgcggaaaa ttttcgctgt cgttgttcaa taccaccggc atggtctccg
     2161 catcttccgc atcgctgcgg caatgcggga tgaagcctgt ggccggatac tgccatagca
     2221 gcttgtccag ctcttcggag ctggctgcat ccggcgtgct gagcacgacg cgcacgccgt
     2281 tctgcatggc cttgtgactc aattgacaag cggtgcgcag cttgtcggtc gagccagtgt
     2341 agaaatcgac cttggtcatt acttctgtgc gcggttgatc aggtattgcg tgagcagtgg
     2401 taccggccgg ccggtcgccc ccttttcctt gccggattta ttggcggtac ccgcgatatc
     2461 cagatgcgcc caacggtaat ccttggtgaa tcgcgacagg aagcaggctg cggtgatggt
     2521 gccgcccgcg cgtccaccga tgttctgcat gtcggcgaag ttgctgtcca gcagtggctg
     2581 gtattcgtcc catagcggca actgccagat gcggtcgttg gattgtgcgc tggcctccag
     2641 cagctccttg gccaagtcat cctgattgct gaacatgccg ctggccacgt tccccagcgc
     2701 gatcacgcag gcgccggtca gtgtggcgat atcgatcacg gtgtccggtt tgaacttggc
     2761 cgagtaggtc aaggcatcgc acaggatcaa acggccttct gcgtcggtgt tgagaatctc
     2821 gatggtctgg cctgacatcg acgtgacgat atcgcccggt ttggtggcgc gaccggaggg
     2881 caggttttcg caggcaggga tcacgccgac tacgttcagc ttgaggccca tctcggcgat
     2941 cgcctgcagg gtgccgagta cgctggcggc accgcacatg tcgtatttca tctcgtccat
     3001 ctcggcgccc ggtttgagcg agatgccgcc ggaatcgaag gtgacgccct tgccgaccag
     3061 cacgatgggt ttctgtttct tgtccgcgcc gtggtattcc atggtgatca gcttggcggg
     3121 ttgttcgctg ccacgggtga cggacaggaa agaacccatg cccagcttct gcatgtcttt
     3181 ctcttccagc acggtggtct tgatattctt gtgtgccttg cccagagcca gcgccttggc
     3241 ggcgaggtag gacggggtgc agatgttgcc tggcaggttg cccagcgtct tggtgagctc
     3301 catgccgtgc gcggtcgcga cagcctgatc gagcgcccgt tgtgcggcgg agtcgggctt
     3361 ggcggcacaa cccagcacga tcttgtgcaa ggtcgaggca tcggcaggtt tgctcttgag
     3421 ttgatcgctg cggaaatccg attcggcggc ggccagaacg gcttgcttga tccgccagtt
     3481 ggcatcgcgt cccttgacat caaggtcggt tagaaacagg gctgcatcct tgctggcagt
     3541 cttctgcaag gcggcaaagg cggcgcgcaa ggcatccaga taggctttgt cggcgaattc
     3601 ggcagccttg cccaatccga ccagcagcac gcgctctgcg gtgatgcctg cgacatggtg
     3661 caacatcagc gtgttgccag ccttgccggt catgtcgccg cgtgcgatga catcgctcag
     3721 atggtgtgcc gctgccttgt ccaggtcttg ggccgcagca gaaagtttgc cgccgtcata
     3781 gacaccaacc acgacgcagc cgctacgcag cttttccgga ctgccttgtt ttatgctaaa
     3841 ttccacgcgt tgctccttaa tgatgttcaa acctgttttc cgatgccgga ttatccgcaa
     3901 actcttcgca caaagtcaaa acaacctcgc atgggggtgt ttcaacgcgc gctgataggc
     3961 gagcttacca gcaacgggct gctggtgttc gcggtgttgc tgggtatcgt ggtggtcagt
     4021 cagttgatcc ggctgctggg ggatgccgtc agtggccgga tcgccgtgga tggggtgctg
     4081 gctctgctcg gtttcgcagc catgaattat ttgccggtgt tgctttcgat cagcctgttt
     4141 atttccatcc tgctgaccat ttcgcgcagc tatcgcgaca gcgagatggt ggtgtggttc
     4201 tgtgccggta tcggactgac gcgctggata cgaccggtga tgttgtatac cttgccgata
     4261 gtcggattga tcgcgttgct gagtctgttg ctgtcgccct gggcgttgca gaaggccgag
     4321 gagttcaagc gcaagctgga gagccgcgac gatgtggcgg ccgcgattcc gggcaccttc
     4381 cgcgagtccg gacaggcgga tcgagtctac ttcatcgaga aagtggatat cggctccaat
     4441 cgtgtcggaa acgtgttcgt gcagtcgctg cagaacggca agcagggcac gatggtggcc
     4501 agtcaaggac tgcaggagac gtcgccgaac ggtgatcgtt ttctggtgct gctcaatggc
     4561 acgcgttatg aaggcacgcc aggaaagctg gattacaaca tcgtggactt cgaacgttat
     4621 tcgatccgta tcgacagttc tccgcccaag caggcgaaca catgggtgcg cagcatgtcc
     4681 acgccggagc tgttgcagga tgaaagtctg tgggcgcgtg ccgagctgga ctggaggctg
     4741 ggactgccga tcagcgccgc aatccttgcc ttgttggcta ttccgctgag ttatgtcaat
     4801 ccgcgggccg gccgctcgct gaatatggtg ctggcgatcg tgctgtatat gctctacagc
     4861 aacatgatca gtgtaagcaa tagctgggtt gcgcagggaa aactgtccca tctcatcggg
     4921 ctttgggcgg tgcatctgat tatgttggct ataacggcgt tgatgtttca gcgtcgcatg
     4981 agcctgctct cgtggcgcag gctgttcggg aaaagaggaa gcatgaggcc ggggcgtaca
     5041 tgaacttgtt gacccgctat ctcgggagag agatatacct cagcatcgcg ctggtgtttt
     5101 ccgcactgat cgccttgttc gccttcctcg acatggttcg ggagctgagc gtaatgcagg
     5161 ggcagtacaa cttgagctat gtactgctgt tcgtggtgtt gactatcccc ggccatattt
     5221 acgaactgtt cccggtcgcg gtactggtgg gtactatctt tgcgatggtg cagatggccg
     5281 ccagttccga gctgaccgtt tatcgcgctt cgggcgcatc gttgtggcag atgatcggtg
     5341 cgctgttcaa gatagcgtta ccgttggtgg cgttgagttt tgtgtttagc gagttcgtcg
     5401 cgccaccgag tgagcgattg gctcaggagc tgcgccagaa ggcccagaat gcgcaggtcg
     5461 tgtttgcaga gtttcgttcc ggcacatggg tgaaagacga acgcagtttc gtgaatgtga
     5521 agacggtgat gccggatggc agtctgacgg aagtcgatat ctatcgtttc gacgagacta
     5581 atcatcttca ggccattact catgccagtc aggcgcgctt tgtcgaacag ggacgctggc
     5641 agctggccgg ggtgcgcgag actcgtttcg acaaacaagg catcagtgtt cgtgctgtgg
     5701 atgagcagga atggccctcg gctttgaatc caggtatttt gagcgtattg ctggtggtgc
     5761 cggagcagat gtcggcttac gacctgtatc agtacaccgt gcatttgaaa gaaaaccgac
     5821 agcagagtgc gcgctacgat atcgccatgt ggggaaagct ggtctacccg ttcgcactac
     5881 tggtgatgat gattttggcg ctgccgtttg catcctatcg tcgtcgttcc ggtggcgtca
     5941 gtgcgatggt attcatgggc atcgtgttag ggctggtatt ccactttgtc ggccgcttgt
     6001 ttgccagctt gggagcactg aacgattggc agccattcat cagtgcggca gcgatgagcg
     6061 tgttgtttct gttcttgggg ctggcgatgc tgtggtggac cgagaggcgc taaccagtct
     6121 gtt
//
LOCUS       DASUIV010000143         9407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_777714, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000143 DASUIV010000000
VERSION     DASUIV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777714"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..945)
                     /gene="gspN"
                     /locus_tag="VFW59_05345"
     CDS             complement(202..945)
                     /gene="gspN"
                     /locus_tag="VFW59_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX5363671.1"
                     /translation="MARWHLAVIGLAIYLLALMIAAPATLVDAGLRRISDGRLLLAEA
                     QGSIWSGSGLIEMRDAGGRTAVAKSIAWRILPTSLLRGHLLCEVELEHSARYFPVTLS
                     LTRLELENADIHLPAAVLGVAVSKLAALGLTGEVSMHVASLSVGPAELRGKGTLQWRD
                     AGSAYTPVSPLGDYELNLDADGTTAQIRLHTLQGPLQLDGNGAWMNGGRPEFIAIARV
                     PPEHRQQLNPLLHLIGMERGSGEFELRFQ"
     gene            complement(945..1433)
                     /gene="gspM"
                     /locus_tag="VFW59_05350"
     CDS             complement(945..1433)
                     /gene="gspM"
                     /locus_tag="VFW59_05350"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX5363672.1"
                     /translation="MRTKLRRIWESRAPRERKVIAIMTMVLAAALYVWLVQSGGQAHT
                     QLRASVTTLRGQAGRLELQAAELERLRATPTMPVSQTNLLTLVQAQTAASGLSHALVK
                     IDAPDADRVAAVFDAVAFADWLNWVAALQSVQVRLDSCRVQVLSAPGMVSVTATLLRT
                     KP"
     gene            complement(1430..2662)
                     /gene="gspL"
                     /locus_tag="VFW59_05355"
     CDS             complement(1430..2662)
                     /gene="gspL"
                     /locus_tag="VFW59_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEX5363673.1"
                     /translation="MSLLRIYSSLFRNTPRDDQTQHCLWALIGDHQQVTAGEGRFADL
                     PQRAGRVQLILPATQVLFVHARIPPEARRRGGSVLAFAVEEKMANEPEANQVSWLGAA
                     GDEDVLAVVDKSGLEHLQQAIASAGIHVDEVHCEALLLPVQAGEWSLAWNGSDGFVRS
                     GEFEAAAMDCGDRESPPLSLQLMLKEAKARDAAPASLALYVTAPDVLPNIPSWQDALG
                     VALHIAGTWDWRSAAPDAGIALVKQRQRWRNFPDMAKRLRPAAWILGAALTLHAVALV
                     VDWSLLASDQRNLRQHLDARFRAAFPDAVAVVDPVLQMRRKLAEARHAAGLPDGGDFL
                     PMIERVNAAAQQLPAGMLRTVSYESGRMTLEFAADDKATGPRIVASLIQSGLSVDALP
                     SGVPRSAGGPVIITVRAL"
     gene            complement(2659..3567)
                     /gene="gspK"
                     /locus_tag="VFW59_05360"
     CDS             complement(2659..3567)
                     /gene="gspK"
                     /locus_tag="VFW59_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEX5363674.1"
                     /translation="MRAKQHGVAIVLAMGVVTLAALTATAIMVSQSTWFRQNELTADH
                     VQAQTLTQVGVDWTRALLSDDRRASSVDYLGEPWALRLPPMPVENGELAGRINDQQGS
                     FNLNNLVKEGRVNPAQLAHFKKLLSLLDLPPALGDTLADWLDADGEPQGGAEDAVYLA
                     MRPPYLAANRPLIDVTELVLVSGFDDGVRARLRPFVSALPIFTPVNVNTAPPEVIAAV
                     IEGLGLDEARTLVQKRGLSYFRDRSDFQDRLPKNIQPAAEDISFSSDYFLVQLRATIG
                     SAESSSVALLARTGNNWPAIVWRKIL"
     gene            complement(3564..4181)
                     /gene="gspJ"
                     /locus_tag="VFW59_05365"
     CDS             complement(3564..4181)
                     /gene="gspJ"
                     /locus_tag="VFW59_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HEX5363675.1"
                     /translation="MSNPAGARSTSCGFTLIELLIALLMLSILALMSYRGLGAVLDAR
                     EHIAGETGKWRQVSVFLMRFERDVQMAAPRPVRTVSGSAPAWLARPDTASHSLNSPQL
                     EFSRFASTEGIDAPRRIAYRLNERQEIELWLWSGLDMPPDALPERYPVLMGVEKFELK
                     YLNASFAWVDAWPTMPSDTPIPRAVKLRITLASGEEIMRIFALHS"
     gene            complement(4178..4573)
                     /gene="gspI"
                     /locus_tag="VFW59_05370"
     CDS             complement(4178..4573)
                     /gene="gspI"
                     /locus_tag="VFW59_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEX5363676.1"
                     /translation="MIVWCSAKRDGFTLVEVLVALAIVSVALLASLRVAGVGTNSVGE
                     LRAHLLAAGVADNVLAEQRARGVWLPIGMQHGTEQQGGIDYFWREEAVATPNPSFRRV
                     DIRVYAESGEAHEIGHLVGFLVQQPGIEK"
     gene            complement(4551..5135)
                     /locus_tag="VFW59_05375"
     CDS             complement(4551..5135)
                     /locus_tag="VFW59_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX5363677.1"
                     /translation="MNKPFSHAVLHPPVFFSGADHIAYPAPGKEKESGFTLIEMLVVL
                     MIMGLLIGLVSVIAQPDDRAQLRVEAERLAQLMDLAATESQLGGKPVAWTADAAGYRF
                     WRFSEASGWSELSDATLHARLLPKGMTISNLRVENMKAAGRMRVEFHAYGSAPVFSLE
                     MTLGEARLTVENSPIGEIRILPEGRRPNDRLVQR"
     gene            complement(5151..5582)
                     /gene="gspG"
                     /locus_tag="VFW59_05380"
     CDS             complement(5151..5582)
                     /gene="gspG"
                     /locus_tag="VFW59_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX5363678.1"
                     /translation="MKVTSRQSGFTLLEVMVVVVILGILAALVVPKIISRPDEARVMA
                     AKQDIASMMQALKLYRLDNHRYPATEQGLQALATQPVVAPLPPNWKAGGYIERLPKDP
                     WGNPYQYLNPGVHGEIDIFSNGADGVIGGEGNDADIGSWSL"
     gene            5811..6278
                     /locus_tag="VFW59_05385"
     CDS             5811..6278
                     /locus_tag="VFW59_05385"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX5363679.1"
                     /translation="MQTLSFDRSTIAATVTVTLATLGALALFALVAAHWSWEWLAPPS
                     QPRTQVVEVSGLKSGSADDLFGHLERARNSAASTGIALKLLGLVAATAGRDGYALIQV
                     DSKEVRTVREGAEIAPGIRLAEVGTNHVILERSGMRETLAWPEKNSSAALIGP"
     gene            6329..8470
                     /gene="gspD"
                     /locus_tag="VFW59_05390"
     CDS             6329..8470
                     /gene="gspD"
                     /locus_tag="VFW59_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX5363680.1"
                     /translation="MKYCWLKAGIVSASMVIAIGQAWALDEPARPAAPAVGGAAAPVG
                     PDVVTLNLVNVAIEDVVKVVSEITSKNFVLDPRVKGTVNIVSSKPMLRSAVYDVFVSA
                     LRLQGFAVVEDYGMVKIIPEADAKLNSGPTFGPSEQGHGAGDRIQTRIYTLKYESAAQ
                     VVPILRPLISPNNTITAYQNSNTLVITDYANNLKRLEKIIDSIDQPNSNDPVVIPLAY
                     ASAVDVAATVTRLFAETSPSQNAADPIQRFAVIADTRSNSLLVRSGDPSRLTQLHKFL
                     TILDSPTNAGGNIHVVYLKNADAVKLAETLRAIYQNEAGTASLPRVSVAAPTALGASA
                     QAGAITATGTSQTAANSPGIIQADASTNSIIITAPDAIYNNLRAVLDKLDARRAQVYV
                     EALIAEVTADKAAEFGIQWQNLSGLGKNNAQAFGGTNFGNAGQNIAGIAQNPATASPG
                     LNVGIVKGQVTIPGVGQILNLGLLVRALESDVNANILSTPTLLTLDNEEASIVIGQNV
                     PFITGQYAMSGAATTPTPFQTVDRRDVGLTLRIKPQISEGGTVRLQMYQEVSSVDKAS
                     TNPAGVTTNKRAVSSTVLVDDGQIVVIGGLIQDSISDGEQKVPLLGDIPLLGGLFRYR
                     TRSHSKTNLMIFLRPTVVRDSQRANTYTGERYDYILGEQARVKPVHDMVLPEMASPTL
                     PPRLPAAPPAAQPATTVKPAAEVPQTGPGGA"
     gene            8470..>9407
                     /locus_tag="VFW59_05395"
     CDS             8470..>9407
                     /locus_tag="VFW59_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5363681.1"
                     /translation="MNRAALKIPYAFANTCGVVVTALSDEMAEVAVRNDAGPGTLAEL
                     RRVLGVPLRTRLVDSSIFDTLLADAYNAADIGAAALAGDLAQELDLSRLLQEIPKIED
                     LLESQDDAPVIRLINALFTQALRDGASDIHIEPFETRSVVRLRIDGSLRDLIESAPGL
                     HGAIVSRIKIMAKLDIAEKRLPQDGRIALRVAGKPVDVRVSTIPTGYGERVVLRLLDK
                     QAGRLDLAKLGMDDAELAHIDSLIKQPHGIVLITGPTGSGKTTTLYAALSRLDATSLN
                     IMTVEDPIEYTLDGISQTQINTKIEMTFARALRTILR"
BASE COUNT         1918 a         3025 c         2750 g         1714 t
ORIGIN      
        1 tctggaagat gtactggccg aaatcgagcg tcttcaacaa cgcgacaagg ccgccaccct
       61 gcacggcaga aaatcggggt agcgcgggga tatcctgatt actcgtcatt cgagcccctt
      121 tcgtcccgaa gcctgctcta accgtttcag ctgcagagct aaagatggcc gggcaaggtg
      181 gcatgacgat gcccgatcgg tttactggaa gcgcaactcg aattcgcctg aaccgcgttc
      241 catcccgatc aggtgcaaca gcggattgag ttgctgccgg tgctctggtg ggacacgggc
      301 gatggcgata aattcgggcc tgcccccatt catccaggcg ccattgccat cgagctgcag
      361 cggcccctga agggtatgca gccggatttg cgcagtcgta ccatcggcat cgagattcag
      421 ctcatagtct ccgagcggag aaacaggggt atatgccgac cccgcatcgc gccattgcag
      481 cgtgcctttc ccccgcagct ctgcaggccc gaccgacagg ctggcaacat gcatcgacac
      541 ctcgccggtc agtccaagcg cggccagttt gcttaccgcc acccccagca ccgcagcggg
      601 cagatggatg tcggcgtttt ccagctctaa ccgggttaac gatagcgtca ccggaaaata
      661 tctggccgag tgttccaact cgacttcgca aagcagatgt ccgcgcaaca gcgaggtcgg
      721 cagaatccgc caggcgatac tttttgcgac agcggtacgc ccgcccgcat cgcgcatctc
      781 gatcaggccg gagccagacc atatcgagcc ctgcgcctcg gccagaagca gcctgccatc
      841 gctgatgcgc cgcaagcccg catcgacgag cgtggcgggt gccgcaatca tcaaagccag
      901 aaggtaaatc gccagcccga taacggccag atgccagcgt gccatcaagg cttggtccgc
      961 agcagggttg cggtcacgct gaccatgccc ggcgcagaca gaacctgcac gcgacagctg
     1021 tcgagtctga cttgcacaga ttgcagggcg gcaacccagt tgagccaatc cgcaaatgca
     1081 accgcgtcga atactgcggc aacccgatcg gcatccggcg cgtcgatctt gaccagcgcg
     1141 tgcgacaagc cggacgcggc cgtctgggct tgcaccagcg tcagcagatt cgtctgcgat
     1201 accggcatgg tcggcgtggc gcgcaatcgc tccagctctg ccgcctgaag ttcgaggcgg
     1261 cccgcctgcc ctcgcaaagt cgtcacgctt gcgcgcaact gcgtatgagc ctgacctccc
     1321 gactgcacca gccacacata caacgcggcg gccagcacca tcgtcatgat ggcgatgacc
     1381 ttgcgttccc ggggggcacg cgattcccat atcctgcgca gcttcgttct cataacgccc
     1441 tcactgtaat aatgaccggg ccgcctgcgg atcgcggcac accggacggc aaggcgtcga
     1501 cactcagacc ggactggatc agacttgcca cgatgcgtgg tccggtcgcc ttatcgtcgg
     1561 cggcaaactc cagcgtcatc cgtccgctct cataggacac cgtgcgcaac ataccggcag
     1621 gcaactgctg cgcggcggca ttcacacgtt cgatcatcgg caggaagtcg ccgccatcgg
     1681 gcagcccggc ggcatggcgg gcttctgcga gtttgcggcg catctgcaac accggatcaa
     1741 ccaccgcaac cgcatccgga aaggcggccc ggaaccgcgc atccaggtgc tggcgcaagt
     1801 tgcgctgatc gctggcgagc agcgaccaat caacgaccag cgcgaccgca tgcaaggtca
     1861 gcgcggcgcc cagtatccag gccgcaggcc gcaatcgttt ggccatatcg ggaaagttcc
     1921 gccagcgttg gcgctgcttt accagtgcga tgcccgcatc cggcgctgcg ctgcgccaat
     1981 cccatgttcc tgcgatatgc agtgccacgc ccagcgcgtc ttgccacgac gggatgttcg
     2041 gcagaacatc cggcgcggtg acatagagcg ccagcgatgc cggtgcggcg tcgcgcgctt
     2101 tggcttcttt caacataagc tgcaaagaca agggcggcga ttcacggtca ccgcaatcca
     2161 tcgcagcagc ctcgaattcc ccgctgcgga caaaaccgtc gctgccattc caggcaaggc
     2221 tccactcgcc cgcctgcacc ggcagcaaca gggcttcaca gtgaacctcg tcgacatgaa
     2281 tgccggcgct ggcaatcgct tgctgcaagt gctccagccc cgacttgtcg accaccgcga
     2341 gcacatcttc gtcgccagcg gcacccagcc agctcacctg attggcttcc ggctcgttgg
     2401 ccattttttc ctccaccgca aaggccagca ccgatccgcc ccggcgtctg gcctcgggcg
     2461 gtatgcgtgc atgcacgaac agcacctggg ttgcaggcaa tatcagctgg acgcgcccgg
     2521 cccgttgcgg cagatcggca aaccggcctt cacccgcagt cacttgctga tgatccccga
     2581 tcaatgccca tagacagtgc tgcgtttggt catcccgggg cgtgttgcga aaaagagaac
     2641 tgtagatacg aagcaaactc ataagatttt tctccagaca atggcaggcc agttgttgcc
     2701 ggtgcgcgcg agcaacgcca cactgctcga ctcggcactg ccgatggttg cgcgcagttg
     2761 caccagaaag taatcgctgc tgaacgagat atcttcagca gcaggctgaa tattttttgg
     2821 caaccgatcc tgaaaatcgg aacggtcacg aaagtaagaa agtccgcgct tctgaaccag
     2881 cgtacgggcc tcgtccagac ccagcccctc gatgaccgcc gcaatgactt cggggggggc
     2941 ggtgtttaca ttgaccgggg taaagatcgg caaggccgat acgaaagggc gcaaacgtgc
     3001 gcgcacgcca tcgtcaaaac cgcttaccag caccagctcg gtcacgtcga tcaggggccg
     3061 gttggcggcg agataaggcg gccgcatcgc cagatagacc gcatcttcgg caccgccttg
     3121 cggctcgccg tcggcatcca gccagtctgc cagcgtgtcg cccagcgcgg gcggcagatc
     3181 cagcagggac agcaactttt taaaatgggc gagctgcgcg gggttgaccc tgccctcttt
     3241 caccagatta ttcagattga atgagccctg ctggtcattg atacggcccg ccaactcgcc
     3301 attctcgacc ggcatcggcg gcaaccgcag cgcccagggc tcacccagat aatcgacgct
     3361 gcttgcgcgg cggtcatcgc tcagcagggc gcgcgtccag tccaccccga cctgggtcag
     3421 cgtttgcgcc tgaacgtgat cggcagtcag ttcgttttgc cggaaccagg tactctgcga
     3481 caccatgatc gccgtggcgg tcagcgcggc gagggtgacc acgcccatgg ccagcacgat
     3541 agcgacgccg tgttgtttcg ctctcatgaa tgcaacgcga agatgcgcat gatctcttcg
     3601 ccggaagcga gcgtgatacg caatttcaca gcccggggaa tcggcgtatc cgacggcatg
     3661 gtcggccatg cgtctaccca tgcgaaactg gcattgagat atttcaactc gaatttttct
     3721 acgcccatca gcaccggata acgctccggc agcgcatccg gcggcatatc cagtcccgac
     3781 cacaaccaca gctcgatctc ctgccgctca ttcaaccggt aggcgatccg gcgcggcgca
     3841 tcgatgcctt cggtcgaggc gaagcggctg aactccagct gcgggctgtt aagtgaatgg
     3901 gatgccgtat ccggccttgc cagccatgcg ggtgcgcttc cggagacggt gcgcaccggt
     3961 cgtggcgcag ccatctgtac atcccgctcg aagcgcatga gaaaaacgct cacctgccgc
     4021 cactttccgg tttctcccgc gatatgttcc cgcgcatcaa gcacggcacc gagtcctcgg
     4081 taagacatca gcgccaggat cgagagcatc agcagtgcga tcagcaactc gatcagcgtg
     4141 aatccgcatg aagtggagcg cgccccggct ggattgctca tttttcgatt cccggttgct
     4201 gcaccaggaa cccaaccaga tgcccgatct catgcgcctc gccagattcg gcgtaaacgc
     4261 ggatatcgac gcgtcgaaac gatggattgg gtgtcgcaac ggcttcctcg cgccagaaat
     4321 aatcgatgcc gccctgttgc tcggtgccgt gctgcatgcc aatcggcagc cacactcctc
     4381 gggcgcgctg ctccgccaac acattgtccg ccacccctgc ggccaacaga tgagcgcgca
     4441 gctcgccgac gctgttggtt ccgaccccgg ccacgcgcag agaggcgagc agcgcgaccg
     4501 acacgatggc cagcgcgacc agcacctcga ccagcgtaaa gccgtcgcgt ttagcgctgc
     4561 accagacgat cattcggtct gcgtccttcc ggcaagatgc ggatctctcc gatcggggag
     4621 ttctccaccg tgagccgtgc ctcgcccagc gtcatttcga gactgaaaac gggtgccgaa
     4681 ccgtaggcat gaaattccac gcgcatgcgt ccggctgctt tcatgttttc cacgcgcagg
     4741 ttcgaaatcg tcatgccctt gggcagcagc cgcgcatgca gagtggcatc ggaaagctcg
     4801 gaccaccccg atgcttcgct gaaacgccag aaccggtagc ccgccgcgtc ggctgtccag
     4861 gcaacgggtt tcccgccgag ctgggattcg gtcgcggcaa gatccatgag ctgcgccagc
     4921 cgttcggctt cgacacgcag ctgcgccctg tcgtccggtt gcgcaatgac gctgaccaga
     4981 ccgatcaata gccccatgat catcagcacg accagcattt cgatgagggt gaatccggac
     5041 tctttttcct ttccgggtgc cggataagcg atgtgatccg ctccactgaa aaaaacaggg
     5101 ggatgaagta ctgcatgact gaacggctta ttcatgcgtt gacccttcgt ttaaagcgac
     5161 caggagccga tatcggcatc gttaccttca ccgccgatca caccatcggc cccgttgcta
     5221 aaaatatcga tctcgccatg cacgccggga ttcaaatatt ggtagggatt gccccatggg
     5281 tctttcggca ggcgttcgat atatccgccc gctttccagt tgggtggcag cggtgcgaca
     5341 acaggctgcg tagccagtgc ctgcaagccc tgttcggtag cggggtaacg gtgattgtcc
     5401 agccgataaa gtttgagtgc ctgcatcatg ctggcaatgt cctgcttggc ggccattacc
     5461 cgcgcctcgt ccggacggct gatgattttc ggcaccacca gagcggcgag tatcccaagg
     5521 ataacgacga caaccattac ttcgagcagg gtaaacccgg attggcgact tgtgactttc
     5581 attggtgctc catgtaggac atcgatatca gagcgattct gcgttgcgat cacgtcaggc
     5641 tttgcgatta tccggtttgc ctctgtccgc tctgttcgct ggcgtacata caccgccatg
     5701 cccaggattt agtcttgccg ccaagttggc gtattgcgtc cggctcgatg cggcttcatc
     5761 cggttgcccc gcgtcgcttc cccttagttc aggtacacca ataacgaaag atgcagacgc
     5821 tctcatttga caggtcaacg atcgcggcaa cggtcaccgt tacgctggcg acccttggcg
     5881 cgctggcttt gttcgcactg gtcgcggcgc actggagctg ggaatggctg gcgccgccgt
     5941 cgcaaccgcg cacgcaggtc gtggaagtca gcggattgaa aagcggatcg gccgacgatc
     6001 ttttcgggca tctggaacgg gcccgcaaca gcgcggcatc cacgggaatt gcgctgaaac
     6061 tgctcgggct ggtggccgcc acggcagggc gggatggcta cgcattgata caggtggatt
     6121 cgaaagaagt tcgcaccgtg cgggaaggag cggagattgc tcccggcatc cggctggccg
     6181 aggtcggcac caatcacgtc attcttgagc gaagcggcat gcgcgaaacc cttgcgtggc
     6241 cggaaaagaa cagttcggcc gcgttgatcg gcccttgaat cggcagacaa cgatcaccgg
     6301 cgttacaaat tgcatgtcag gagttgaaat gaagtattgc tggttgaaag ccggaattgt
     6361 atcggcaagc atggtgatcg ctatcgggca ggcgtgggcg cttgacgagc ccgcccgtcc
     6421 cgccgcgccc gcagtcggcg gcgcagcggc gcccgtcggg ccggatgtgg tcacgctcaa
     6481 tctggtgaat gtcgccatcg aggatgtggt gaaagtagtc agcgagatca ccagcaagaa
     6541 cttcgtgctc gatcccaggg tgaaaggcac ggtcaatatc gtttcgtcga agccgatgct
     6601 gcgatccgca gtctacgatg tattcgtgtc ggccttgcgt ttgcagggct tcgcggtggt
     6661 cgaagattat ggcatggtaa aaatcatccc cgaagccgat gccaagctga attcgggccc
     6721 caccttcggc ccctcggagc aggggcacgg cgcgggcgac cggatacaga cccggatcta
     6781 tacgctgaaa tacgaatcgg ccgcccaggt cgtgcccatt ctgcgtccgc tgatctcccc
     6841 caacaacacc atcacggcgt accagaacag caacaccctg gtgatcaccg actacgccaa
     6901 caacctcaag cgccttgaga agatcatcga ttcgatcgat cagcccaaca gcaacgatcc
     6961 ggtcgtgatc ccgctggcct acgcctccgc agtcgatgtt gcggcgaccg tcacgcgcct
     7021 gttcgccgaa acctcgccgt ctcagaacgc ggccgatccg atacagcgat ttgcggtgat
     7081 tgcagacacg cgttccaaca gcctgctggt gcgttccggc gacccttcac gtctcaccca
     7141 gctgcataaa tttctgacga tactggattc gcccaccaat gccgggggca acattcacgt
     7201 ggtctacctg aaaaatgccg atgcggtgaa attggccgaa acactgcgcg ccatttatca
     7261 gaatgaggcg ggcaccgcat cgctgccaag ggtatctgtt gcagccccaa cggcactcgg
     7321 cgcatcggca caggccggcg cgatcacggc aaccggcacg agtcagactg ccgcaaattc
     7381 acccggcatc attcaggccg acgcatccac caactccatc atcatcaccg cacccgatgc
     7441 gatctataac aatttgcgcg cggtactgga caagctggac gcgcgccgcg cgcaggtcta
     7501 tgtcgaagcg ctgatcgcgg aggtcacggc cgacaaggcc gcagagttcg gcatccagtg
     7561 gcagaacctg agcggtctgg gcaaaaacaa tgcgcaggct ttcggcggca ccaacttcgg
     7621 caacgcgggc cagaacattg ccggcattgc gcagaatccg gccacggcct cgcccggact
     7681 gaatgtcggc atcgtgaaag gacaggtgac cattcccggc gtcggccaga tactcaacct
     7741 cggcctgctg gtgcgcgcgc tggaatccga cgtcaacgcc aacatcctgt ccacgccgac
     7801 gctgctgacg ctggacaacg aagaagcctc catcgtcatc ggccagaacg tcccgttcat
     7861 caccgggcag tatgcgatgt cgggcgcagc caccacgcct acgccctttc agacggtgga
     7921 ccggcgcgat gtcggcctga ccctgcggat caagccgcaa atctccgaag gcggaaccgt
     7981 gcgcctgcag atgtatcagg aggtctccag cgtcgacaag gcctcgacca acccggccgg
     8041 tgtcaccacc aacaagcgcg cggtgtcatc gacggttctt gtcgatgacg ggcagatcgt
     8101 ggtgatcggc gggctgatac aggattcgat cagcgacggc gaacagaagg ttccgttgct
     8161 cggcgacatt ccgctgctgg gcggcctgtt ccgctaccgg acacgctcgc acagcaagac
     8221 caatctgatg attttcctgc gacctacggt ggtcagggat agccagcgcg cgaacaccta
     8281 caccggcgag cgctacgatt acatcctggg cgaacaagcc agggtaaagc cggttcacga
     8341 tatggttctg ccggaaatgg cttcgccgac actgcctccg cgcttgcctg cggcaccgcc
     8401 ggcagcccag cccgccacga ccgtcaaacc ggccgcagaa gtgccgcaga ccggccctgg
     8461 cggagcataa tgaaccgggc cgccctcaag ataccctatg cgtttgccaa tacctgcggc
     8521 gtggtcgtca cggcactgag cgacgagatg gctgaagtgg cggtgcgcaa cgatgccggc
     8581 cccggaacgc tggccgaatt gcgccgtgtg ctcggcgtcc cgctacgtac ccgccttgtt
     8641 gattcctcca tcttcgacac cttgctcgcc gacgcctata acgctgcgga tatcggcgcg
     8701 gcggcactgg ccggcgatct ggctcaggaa ctggatctat ccagactgct gcaggaaatt
     8761 ccgaaaatcg aggatctgct cgagagtcag gacgatgcgc cggtgatccg cctgatcaat
     8821 gccctgttca cgcaggcact acgcgacggc gcatcggaca ttcatatcga gccgtttgaa
     8881 acccgctcgg tggtgcgtct gcgtatcgat ggctcgttgc gcgacctgat cgagtcggcg
     8941 cccgggctgc atggcgcgat cgtgtcgcgc atcaagatca tggccaaact cgacatcgcc
     9001 gaaaaacgcc tgccgcaaga tggccgcatc gcgctgcgcg tggcgggaaa gccggtcgat
     9061 gtgcgcgtct ccacgatccc cacagggtac ggagaaagag tggtgttgcg cctgctagac
     9121 aagcaggccg gccgtctcga cctcgcgaaa ctcggcatgg acgacgccga gctggcgcat
     9181 atagacagcc tgatcaaaca gccgcacggc atagtgctga tcaccgggcc gaccggatcg
     9241 ggcaagacca ccacgctcta tgccgcactc tcaagactgg atgcgacgtc gctcaacatc
     9301 atgaccgtgg aagatccgat cgagtacacg ctcgacggca tcagccagac ccagatcaac
     9361 accaagatcg agatgacttt cgcgcgcgcg ttgcgcacca tcctgcg
//
LOCUS       DASUIV010000144        13070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_591788, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000144 DASUIV010000000
VERSION     DASUIV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 13070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13070
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591788"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..2104)
                     /locus_tag="VFW59_05400"
     CDS             complement(68..2104)
                     /locus_tag="VFW59_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent copper receptor"
                     /protein_id="HEX5363682.1"
                     /translation="MKIKRITICVALAIAPGAFAGEMMALDEVVVTAPAMERPLEVST
                     DPRKPRQPVPAHDGADYLKTIPGFSVIRKGGTDGDPVFRGMAGSRLNILLDGEQILGG
                     CGGRMDPPTAYVFPAAYERITLLKGPQTVLYGPGNSAGTVLFERSSKREAAGVKGNGS
                     LMLGSFGRNDEMAEARAVSESAYLRVGGTRSQANDYQDGSGNAVHSAYNRWSGNAALG
                     WTPDDNTRLELTAAKSDGKAAYADRAVDGSKFARDNLGLKFNRQGMDGLLEGIEAQVY
                     YNYVDHVMDSYSLRAGGNPAGMMAAMNPDRKTMGGRLVGTLQLAEMTQLKLGLDTQDN
                     VHTGRTGAAPGYMGDYTLKPRVQDASFKNVGVFGELHHNVAETRRVIGGLRADSWQAT
                     DSRAMVMRGMMGGVMNPTVNQTRRETLASGFARYEQDVSGASTVFVGVGHTARAPDYW
                     ELFSNESLASASAFNTRPEKTTQLDAGLSYREGALSGSVSAFYSKIADFNLVQSSVNK
                     GTMMMPVNVTVTRNVNATTYGAEAGMAWQLAESWKLDGSLAYVQGSNDTDGTALGQIP
                     PLEARVGVNYDDKVWSFGGLLRGVAAQSRVAVNQGNIAGQDIAASGGFGVFSVNGGWR
                     ASKAVQVTAGADNLFNKTYAEHISRAGTMVSGFTQTRRVNEMGRNLWVKANLAF"
     gene            2269..2541
                     /locus_tag="VFW59_05405"
     CDS             2269..2541
                     /locus_tag="VFW59_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX5363683.1"
                     /translation="MTRMVQCVKLGREAEGLDRPTYPGPLGQRIFDNVSKEAWQGWIK
                     FQTMLVNENRLNLADAQARKYLAGQMENYFFGAETQMPEGYTPPQN"
     gene            2552..4648
                     /gene="ppk1"
                     /locus_tag="VFW59_05410"
     CDS             2552..4648
                     /gene="ppk1"
                     /locus_tag="VFW59_05410"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX5363684.1"
                     /translation="MSKKYAPQLFLNRELGQLEFNRRVLAQAEDSSVPLLERLKYLCI
                     VSSNLDEFFEIRVAGLKEQIKLGSIATSVDGMTAAQTLKQVCDQAHQLIERQYQLFNN
                     DITPALAAQDIKFIRRTHWNEVQQAWVKDFFFREVMPVLTPIGLDPAHPFPRVLNKSL
                     NFAVELQGKDAFGRNSTRAIVQAPRVLPRSILMPPEISGCEHGFVFLSSILHTHVGEL
                     FAGMEVLGCYQFRVTRNSNLFVDEEEIKNLRLSLQGELPQRHFGDAVRLEIADNCSPQ
                     IAELLLKEFALAPQDLYRVHGPVNLVRLMEIPNLVARDDLKFPPFTPSIPNTLQKKGA
                     DMFKLIRKNDVLLHHPYQSFKPVVDFIQQAASDPNVVAIKQTVYRTGTDSALMDALIG
                     AAQRGKEVTVVVELLARFDEEANINWATRLEQVGAHVVYGVVGHKTHAKMLMVVRREE
                     SGLRRYVHLGTGNYHPRTARLYTDFGLFTANEEICADANEVFSQLTSLGKARKLNHLW
                     QSPFTLHSQVLAAIRNETALAKSGKRAHIIAKMNSILEPETINALYEASQAGVKVDLI
                     VRGVCALRPGVKGLSENIRVRSVIGRFLEHTRIFFFRNDLKNDVYLASADWMDRNFFR
                     RVEVCFPVLDKKLKKRVIDEGLKAYLMDNRQAWEMDGNGQYRRKNVRRSAAKCAQVQL
                     LQLLSGTPPQPVSGTN"
     gene            complement(4731..5147)
                     /locus_tag="VFW59_05415"
     CDS             complement(4731..5147)
                     /locus_tag="VFW59_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363685.1"
                     /translation="MATKKNAVIETKSTTSGAPVKVMAEGVKTAAKPVVKAAVRKAAK
                     PVRKAAVEDVSETAPAAKKQKNKVKVVRDSFTMPQDEYQKIAEIKEAGLEAGLSVKKS
                     EVLRAGLKALASMSPAQLKRALAGLEKIKTGRPKKH"
     gene            5228..5680
                     /gene="sixA"
                     /locus_tag="VFW59_05420"
     CDS             5228..5680
                     /gene="sixA"
                     /locus_tag="VFW59_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX5363686.1"
                     /translation="MDLILWRHAEAADGTPDQARELTAKGRKQAEKVAIFLRQHLPKD
                     TRILVSPATRTLQTVAALTEQFTLAPTLAPGASAQAVLQAARWPDAGGSVLIVGHQPT
                     LGEVAAQLLGSNEPSLSIKKGALWWFSRRERDDLAQTTLRLSITPEFL"
     gene            5691..7178
                     /gene="pap"
                     /locus_tag="VFW59_05425"
     CDS             5691..7178
                     /gene="pap"
                     /locus_tag="VFW59_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HEX5363687.1"
                     /translation="MFESAELGHKIDKATYDAEVPKLREALLEAQMDLAKLAKFPVII
                     LIGGIDGAGRGETVNLLNEWMDPRFVETHGMGEPSDEELDRPMMWRFWRALPPKGKVG
                     VFLGSWYTWPILNRVMGRTKTAELDQSLERAKRLEKMLVDEGALVLKFWMHLSKEKQE
                     KRLKLLEKDPKTRWRVTDRDWQHYKMYDKFLAVNESVIRHTSSAEAPWSIVEGYDPRY
                     RSLTIGKIILDSIRKRLDEGGKLPVEVAAPPPLPSIDQLNVLKTLDLNQKLSRKKYEA
                     ELEKYQGKLAVLTRDPKFRDITVVVLFEGNDAAGKGGAIRRITGALDARHYNIVPIAA
                     PTEEERAQPYLWRFWRHIPRRGRLTIFDRSWYGRVLVERVEGYCSEADWMRAYSEIND
                     FEAQLVRHKIVVVKFWLSISKEEQLRRFKEREKIGFKRFKITEEDWRNRKKWDEYEHA
                     VCDLVDFTSTEIAPWTLVEANDKYFARIKILKTLCQQIESAIKKK"
     gene            7253..8263
                     /locus_tag="VFW59_05430"
     CDS             7253..8263
                     /locus_tag="VFW59_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEX5363688.1"
                     /translation="MTRHNLLSLSLALGAIFVGINIAQAAASNEPIQPIAAAKPKNVA
                     VVELGKKLYFDPRLSKSGFISCNSCHNLSMGGTDNIKTSIGHKWNQGPINAPTVLNSS
                     MNLAQFWDGRAADLKAQAGGPIANPGEMASTHALAIDVLQSIPGYATEFKTAFGSDKI
                     DIDKVTTAIAAFEETLVTPNSRFDKWLKGDKKALNKQEVAGYQLFKDSGCSACHNGPA
                     VGGNSFQKLGMVEPYQTTNKAEGRSGVTGKGEDRNVFKVPTLRNIEMTYPYFHDGEVA
                     TLKDAVDTMGRVQLGKKFSEEENGKIVAFLKTLTGDQPSFKMPILPPSTDKTPRPQPF
                     GN"
     gene            8411..9313
                     /locus_tag="VFW59_05435"
     CDS             8411..9313
                     /locus_tag="VFW59_05435"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5363689.1"
                     /translation="MHWAYLHYPDPRLECPLNQGPFMILPPTLSVEEQLLKAINNDEL
                     ILPTLPDVALKIQQMMDNMDIPASRIVTLVSGDPVIAAQLIKAANSAIYAEKPRIDNV
                     SGAISRLGYRTLRNLVLNITMSRLAKADQPAIKMLLANFWEHSREVAAISHMLAKNQR
                     HLNADQAMLAGLIHDIGTLPLCIYVDKMRSNFDGPLIEGLILQFRGYVGEKLLRTWGF
                     PDEIVEVIPEHENLQRNSESHQASYADIVTVANLMNRLTAKVVPWEDITALQRLHISP
                     DICRNFHERFGDELSTTHEMLFPH"
     gene            complement(9337..9651)
                     /locus_tag="VFW59_05440"
     CDS             complement(9337..9651)
                     /locus_tag="VFW59_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2288 domain-containing protein"
                     /protein_id="HEX5363690.1"
                     /translation="MTESSLQDINRTSINLETSRIAWKELQRFFASGAAVAVSPELDL
                     VDVAFQISEDNRSQVEQWLVAGLVGKVTDEQALAWFEADADVWAVVVNPYVLVQSAER
                     LQ"
     gene            9897..11261
                     /gene="glmU"
                     /locus_tag="VFW59_05445"
     CDS             9897..11261
                     /gene="glmU"
                     /locus_tag="VFW59_05445"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5363691.1"
                     /translation="MSPLNIVILAAGKGTRMYSDKPKVLHTLAGKPLVQHVLDCATAL
                     QPQSVCVIYGHGGEAVPQAMSPYQAKFVIQEPQLGTGHAVQQAMPHLPDGSRTLVLYG
                     DVPLTQHSTLHQMLQAGEGLTLLTVTLDNPTGYGRIVRDAQGDVQCIVEEKDATAEQR
                     EIREVNTGILLAPTGQLREWLARLGNNNAQGEYYLTDIVGMAVRQGVAVHTVQPAQHW
                     EVEGINSKAQLAQLERTWQRVEAGRLLAHGVTLADPARLDVRGKLSCGRDVEIDVGCI
                     FEGDVTLGDGVRVGAYSIVRNASVASGTDIAPYSHIDSSEVGANCHIGPYARLRPGTR
                     LADGAHIGNFVEIKNSEIGQGSKANHLSYIGDSTVGSRVNIGAGTITCNYDGANKHRT
                     VIGDDAFIGSDTQLVAPVTVGKGATIGAGSTITRDTPDQELTLSRSKQLTITGWKRPQ
                     KGKK"
     gene            11263..>13070
                     /gene="glmS"
                     /locus_tag="VFW59_05450"
     CDS             11263..>13070
                     /gene="glmS"
                     /locus_tag="VFW59_05450"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5363692.1"
                     /translation="MCGIVGAVAQRNVVPILLEGLKRLEYRGYDSAGLAVVHEGQLQR
                     VRSTGRVAELTAKSATTAGQLGIAHTRWATHGVPSERNAHPHLSNDHIAVVHNGIIEN
                     YEELRKRLTASGYVFTSDTDTEVIAHLIHSHYVLGLSLLAATQASLVELAGAYAIGVI
                     ASDEPDRLICARRGSPLLLGVGIGEHFIASDVSALLPVTREVVYLEEGDVVELERGGY
                     RIFDASGTLTTRPVQTSEQSNESVELGQYRHYMQKEIHEQPQALADTLESVCNSQSLV
                     PGIFGAEAAATFDDIDSILILACGTSNHAGQVARYWLEEIAGIPCSVEIASEYRYRVS
                     VPNPKALVVVISQSGETADTQAALNHAKAQGHVHTLAICNVPESALVRLSKLRLLTRA
                     GPEIGVASTKAFTTQLAALFLLTLVLAKQRGRLTQQQESQYLHELRHLPSAVQKVLDL
                     EPQIAKLAERFADKHNALFLGRGMHFPIALEGALKLKEISYIHAEAYPAGELKHGPLA
                     LVDKDMPVISVAPNDALLEKLKSNLQEVRARGGELYVFADADTQIREADGIHVMQMPE
                     HAGYLSPILHTIPLQLLAYYVALQKGTDVDKPRNLA"
BASE COUNT         2928 a         4079 c         3523 g         2540 t
ORIGIN      
        1 aaattaaaac caccccaaaa aaaaagccgc ctcttaaggc ggcttttttg ctttcctgca
       61 gtaacgatca gaacgccaga ttagccttca cccacaggtt acggcccatt tcgttgacgc
      121 gcctggtctg ggtaaagccg ctcaccatcg taccggcacg gctgatgtgc tccgcgtagg
      181 tcttgttgaa caggttgtcc gccccggcgg tcacttgcac cgccttgctg gcacgccagc
      241 caccattgac cgaaaacacg ccgaaaccgc ccgaagccgc gatgtcctgt ccggcaatgt
      301 tgccctgatt caccgccacc cggctctgag ccgccacgcc gcgcaacagg ccaccgaacg
      361 accatacctt gtcgtcatag ttgacgccga cacgcgcttc cagcggcggg atctggccca
      421 atgccgtgcc atccgtatcg ttgctgccct gcacataagc caggctgccg tccagcttcc
      481 agctctccgc cagttgccat gccatgcccg cctcggcgcc ataggtcgtg gcattcacat
      541 tgcgggtgac cgtcacgttc actggcatca tcatcgtgcc cttgttgacg ctggactgca
      601 ccagattgaa gtcggcaatc ttgctgtaga acgccgaaac cgagccggac agcgcaccct
      661 cgcgatagct cagccccgca tccagttgcg tagtcttttc cggccgggtg ttgaatgcgc
      721 tcgccgaggc caggctttcg ttgctgaaca actcccagta gtccggcgcg cgcgcggtgt
      781 gtcccacacc gacaaacacg gtcgatgcac cggaaacatc ctgctcgtaa cgcgcgaacc
      841 cgctggccaa ggtctccctg cgcgtctggt tgacggtcgg attcatcacg ccgcccatca
      901 tgccgcgcat caccatcgcg cgggaatcgg ttgcctgcca actatcggca cgcagtccac
      961 cgatcacacg acgggtctcc gccacattgt gatgcagctc accgaacacc ccgacatttt
     1021 tgaacgaggc atcctgaaca cgcggcttga gcgtgtagtc gcccatgtag ccgggcgcgg
     1081 cgcccgtgcg gccagtatgc acattatctt gcgtatccag acccagcttg agctgggtca
     1141 tttcggccag ctgcaacgtg cccaccagcc gtccgcccat ggttttgcgg tccggattca
     1201 tcgccgccat catgcctgca ggattcccgc cggcacgcag gctataactg tccatcacat
     1261 gatcgacgta gttgtaatac acctgcgcct cgatcccctc cagcaaaccg tccatacctt
     1321 gcctgttgaa cttcagcccg agattgtcac gggcgaattt cgatccgtcc accgcgcggt
     1381 cggcgtatgc agccttcccg tcgctcttgg cggcggtcag ttcgagacgc gtattgtcat
     1441 ccggcgtcca gcccaatgcc gcgttgccgc tccagcggtt gtaggcggaa tgcaccgcat
     1501 tgccgctgcc gtcctggtag tcgttagcct gtgaacgtgt accgcccacg cgcagataag
     1561 cgctttccga aaccgcacgc gcttcggcca tctcgtcgtt gcgtccaaaa ctgcccagca
     1621 tcaatgaacc gttacccttg accccagccg cttcacgctt gctgctacgc tcgaacagca
     1681 ccgtacccgc agaattgccc gggccgtaca gcacggtctg cgggcctttc agcagggtga
     1741 tgcgttcata ggcggcggga aatacataag cggtcggcgg atccatgcgc cctccgcagc
     1801 cgccgaggat ctgttcgcca tccagcagga tgttcaaccg cgaacccgcc atgccgcgaa
     1861 acaccgggtc accgtcggtg ccacccttgc ggatcaccga gaagccggga atggttttca
     1921 ggtaatcggc gccatcatgt gccggaacgg gctgacgcgg tttgcgcgga tcggtgctga
     1981 cctccagcgg ccgctccatc gccggtgcgg tcacgaccac ttcatccagc gccatcatct
     2041 cgcccgcaaa cgcgcccggc gcaatagcca gcgcgacaca gatagttata cgtttgattt
     2101 tcatcccttg aacctcccaa taaaaagtgg cgggattatc gatgcggcat cgttcgttgg
     2161 gaatgcggca tattgtcgca cccccgtatt tgccgaacag gcttcgagca aagatgtcat
     2221 cgtgatgtca taatcgcgct acataatcac tgactcggaa agactcacat gacaagaatg
     2281 gtgcaatgcg taaaactggg gcgcgaggct gaagggctgg atcgtccgac ttatcccggc
     2341 ccgctgggac agcgtatctt cgacaatgtc tccaaggaag cctggcaggg ctggatcaag
     2401 ttccaaacca tgctggtgaa cgagaaccgc ctcaatctcg ccgatgcgca ggcgcgcaaa
     2461 tatctcgccg gacagatgga gaattatttc ttcggcgcag aaactcagat gcccgagggt
     2521 tacacgcccc cgcaaaatta aagaggctcg cttgtccaag aaatacgctc cgcaactgtt
     2581 cctcaaccgc gaactcggtc aactcgagtt caaccgtcgc gtccttgctc aggccgagga
     2641 ttccagtgtg ccgttgctgg agcggctgaa atacctgtgc atcgtcagca gcaatctcga
     2701 cgagttcttc gagattcgcg tggcaggcct gaaagagcag atcaaactcg gcagcatcgc
     2761 caccagcgtg gatggcatga ctgcggctca gaccctgaaa caagtgtgtg atcaggcgca
     2821 tcaactgatc gaacgccagt atcaactgtt taacaacgac atcacgccgg cgctcgccgc
     2881 acaagacatc aagttcatcc gccgcaccca ctggaacgag gtgcaacagg cctgggtcaa
     2941 ggattttttc ttccgcgaag tcatgcccgt gctgacaccg atcggcctcg acccggctca
     3001 cccctttccg cgcgtgctta acaagagcct gaatttcgcc gttgagctgc agggcaagga
     3061 tgcgttcgga cgcaactcga cgcgcgccat cgtgcaggcg ccgcgcgtac tgccacgctc
     3121 catcctgatg ccgcccgaaa tcagcggctg cgagcacggc tttgtgttcc tgtcctctat
     3181 tctgcatacg catgtaggcg aactgtttgc cggcatggag gtcttgggct gctaccagtt
     3241 ccgcgtcaca cgcaacagca atctgttcgt ggatgaagaa gaaatcaaaa acctccgcct
     3301 cagcctgcag ggcgaactgc cgcagcgcca cttcggcgat gcggtgcgac tggagatcgc
     3361 cgacaactgc tcgccgcaga tcgccgagtt gctgctcaag gagtttgcgc tcgcgccgca
     3421 agacctgtat cgagtccatg gcccggtaaa tctggtcagg ctgatggaaa tccccaatct
     3481 ggtggcccgg gacgatctca agttcccgcc gttcacgccc agcattccga acacgctgca
     3541 gaaaaagggc gcggacatgt tcaaactgat ccgcaagaac gacgtgctgc tgcatcaccc
     3601 ctaccagtcg ttcaaaccgg ttgtggattt cattcagcaa gccgccagcg acccgaatgt
     3661 ggtcgccatc aaacaaaccg tctaccgcac cggcaccgac tcggcgctga tggatgcgtt
     3721 gatcggtgcg gcgcaacgcg gcaaggaagt caccgtggtg gtcgaactgc tggcgcgttt
     3781 cgacgaagaa gccaacatca actgggccac ccggcttgaa caggtcggcg cgcatgtggt
     3841 gtacggcgtg gtcggccaca agacccatgc caagatgctg atggtggtgc gccgcgaaga
     3901 aagcggactg cgccgctacg tacatctggg caccggcaac taccatccac gcaccgcaag
     3961 gctgtatacc gattttggcc tgttcaccgc aaacgaagag atttgcgccg atgccaatga
     4021 agtattcagt cagctgacca gcctcggcaa ggcgcgcaaa ctcaatcacc tgtggcaatc
     4081 acctttcacc ctgcacagcc aggtgctggc tgccatccgc aacgagactg cgcttgccaa
     4141 atccggcaag cgcgcccaca tcatcgccaa gatgaactcc atactggagc cggaaaccat
     4201 taacgcgctt tacgaagctt cgcaggcagg ggttaaagtc gatctgatcg tgcgcggggt
     4261 atgtgcgctg cgtccgggag tcaaaggatt gtcagaaaac attcgagtgc gctcggtcat
     4321 cggacgcttc ctggagcaca cccgtatctt cttctttcgc aacgacttga aaaacgacgt
     4381 ctacctcgcc agcgcggact ggatggatcg caatttcttt cggcgtgtcg aagtatgctt
     4441 ccctgtgctc gataagaagc tgaagaagcg cgtcatcgac gaggggctca aagcctatct
     4501 gatggataac cgtcaggcct gggaaatgga tggaaacgga caataccggc gcaagaacgt
     4561 acgccgcagc gccgcaaagt gcgcccaggt ccaactgctc caattgctct ctggcacgcc
     4621 accccaaccg gtctcaggga caaactaaac cagcctgcca ttgcagctgg ttcgccaact
     4681 caagactggc caagcaagca cccgcctgtt ggcgggagta tcgcccagaa tcagtgcttc
     4741 tttgggcgcc cggtcttgat cttctccagc cctgccaagg cacgctttaa ttgcgcggga
     4801 ctcatgctcg cgagcgcttt cagaccagcg cgcaacacct cgcttttctt caccgacaag
     4861 cccgcctcca gaccggcttc cttgatttcg gcaatcttct gatactcgtc ctgcggcatc
     4921 gtgaagctgt cgcgcacgac tttcaccttg tttttttgct tcttggctgc cggcgctgtc
     4981 tcggacacat cttcgaccgc agccttgcga acaggcttcg ccgccttccg cactgcagcc
     5041 tttactaccg gcttcgcggc cgttttcacc ccctcggcca tcaccttcac tggtgcgcca
     5101 ctggtcgtgc tcttggtttc gattaccgcg ttctttttgg ttgccatgac atctccttgg
     5161 tattaaagtg acgcaaacag tgtatacggt atatcctgct cagtgaaatg taatattcga
     5221 ggtgataatg gatctgattc tgtggcgaca cgccgaagca gccgatggca cgcccgatca
     5281 agcacgtgag cttaccgcga agggccgcaa gcaggccgaa aaggttgcca ttttcttgcg
     5341 ccaacaccta cccaaagaca ctcgcattct ggtcagccct gccacccgca cgctgcaaac
     5401 cgtcgccgcc ctcaccgaac aattcaccct cgcccccact ctcgctcccg gcgcctccgc
     5461 tcaagcagta ttacaggccg cgcgctggcc tgacgcggga ggctcggtac tgattgtcgg
     5521 gcatcagccg acgctgggtg aagtggcagc acaattatta ggcagcaatg aaccctcgtt
     5581 gagtatcaaa aagggggcat tgtggtggtt cagccgtcgc gagcgcgacg atctggctca
     5641 aaccaccctg cgcttgtcca tcacccctga attcctgtga ggaggtaacc atgttcgaat
     5701 ccgcagaact cggccacaaa atcgataaag ccacctacga tgcggaggtg cccaagctgc
     5761 gtgaagcact gctcgaagcc cagatggatt tggcaaaact ggccaagttt cccgtcatca
     5821 tcctgatcgg cggcatagac ggcgccggtc gaggcgagac cgtcaacctg ctcaacgaat
     5881 ggatggatcc ccgtttcgtg gaaacccacg gcatgggcga gccctccgac gaggaactgg
     5941 atcgccccat gatgtggcgc ttctggcgcg ccttgccgcc gaaggggaaa gtcggtgttt
     6001 tcctcggctc ctggtacacc tggcccatcc tcaaccgagt gatggggcgc accaaaacgg
     6061 ccgaactcga ccagagcctg gagcgcgcca aacgcctgga aaagatgctg gtcgatgaag
     6121 gcgcgctggt acttaaattc tggatgcatc tgtccaagga aaagcaggaa aagcgcctca
     6181 agctgctgga gaaagacccc aagacccgtt ggcgcgtcac cgatcgcgat tggcagcact
     6241 acaagatgta tgacaagttt ctggctgtaa acgagagtgt gattcgccat accagctctg
     6301 ccgaagcacc ctggtcgatc gtcgagggtt atgatccgcg ctatcgcagc ctgaccatag
     6361 gcaagatcat cctcgattcg atacgcaagc gtctggacga gggcggcaaa ttgcctgtcg
     6421 aagtcgcggc cccaccgcca ctcccctcga tcgatcagct caatgtgctg aaaacactgg
     6481 atctgaacca gaaacttagc agaaaaaaat acgaagccga actcgaaaaa tatcaaggca
     6541 agctggcagt gctgactcgc gatccaaaat tccgagacat caccgtcgtc gtcctgtttg
     6601 aggggaacga tgccgcaggc aagggaggcg ccatccgccg catcaccggc gcactggacg
     6661 cgcgccacta caacatcgtc ccgatcgctg cccccactga agaagagcgc gcccagccct
     6721 atctgtggcg attctggcga cacattccgc gtcgcggccg tctcaccatc tttgaccgct
     6781 cctggtatgg tcgggtgttg gtcgagcgtg tcgaaggcta ctgttcagaa gccgactgga
     6841 tgcgtgccta cagcgaaatc aacgatttcg aggcacaact ggttcgccac aagatcgttg
     6901 tagtcaagtt ctggctctcc atcagcaagg aagaacagct tcgccgcttc aaggagcgcg
     6961 agaagatcgg cttcaagcgc ttcaagatca ccgaagaaga ctggcgcaac cgcaaaaaat
     7021 gggacgaata tgagcatgcc gtatgcgacc tggttgattt caccagcacc gagattgccc
     7081 catggacgct agtagaggct aatgacaaat acttcgctcg catcaagata ctgaagacgt
     7141 tatgccagca aatagaatcc gccataaaaa agaaatgacc caggaactct atttcgattt
     7201 tgtactccca atgtagttgt tcttaccaat aactttaaga aaggaagctt ctatgaccag
     7261 acataactta ctgtcacttt ccctcgccct tggggccatt tttgtcggca tcaacatcgc
     7321 gcaagctgcc gccagcaatg agccgattca gccgatcgct gcagccaagc ccaagaatgt
     7381 cgcagttgtg gaactgggca agaaactgta cttcgatcca cggctgtcga agtccggctt
     7441 catttcctgt aattcatgcc acaacctttc gatgggcggc acggataaca tcaagacctc
     7501 gataggccac aagtggaatc aaggcccgat caacgctcca acggtactga actccagcat
     7561 gaaccttgcc caattctggg atggtcgcgc ggcagatctc aaggctcaag ctggcggccc
     7621 gatcgccaat ccgggcgaga tggcttccac ccacgcgctc gcaatcgatg tactgcagtc
     7681 aatcccaggc tacgcaacag aattcaagac ggccttcggc tccgacaaaa tcgacatcga
     7741 caaggtgaca actgccatcg cagcctttga agaaacattg gtgacgccta actcccgctt
     7801 tgacaaatgg ctgaaaggcg acaaaaaagc ccttaacaaa caggaagtcg caggctacca
     7861 gctgttcaag gatagcggct gctccgcctg ccacaacgga ccggccgttg gcggcaactc
     7921 tttccagaag ctgggtatgg tcgagcctta ccagaccacc aacaaggcag aggggcgctc
     7981 gggcgtgaca ggcaagggcg aggatcgcaa cgtcttcaag gtgccgacgc tgcgcaacat
     8041 cgaaatgacc tacccctact tccatgacgg cgaagtcgca accctcaagg atgcggtcga
     8101 caccatgggg cgcgtccagt taggcaagaa attctctgag gaggagaacg gcaagatcgt
     8161 cgccttcctg aaaacactga ccggcgatca gccgtcgttc aagatgccta tcctgccacc
     8221 ttcgaccgac aagacgccac gtccgcaacc cttcggcaac tgattgccgt aagtttcaat
     8281 ccggcggggg cctttgagcc cccgtttttt ttcgccttac ccagtacgag catagcgcat
     8341 tgctctcgca atgactgcca caccagcaca agactaggcc tttatatcta gacaatcaga
     8401 aggattatct atgcattggg catatttaca ttatcccgat ccaaggctag agtgccccct
     8461 taatcagggc cccttcatga ttctgccacc caccctttcc gttgaagaac agctgctcaa
     8521 agcgatcaat aacgacgagc tgatcttacc cacgctgcct gatgtagccc tcaagattca
     8581 acagatgatg gacaacatgg acatccctgc cagcagaatc gtcactctgg tttccggcga
     8641 cccagtgatc gcggcacaat tgatcaaggc cgccaacagc gctatctatg ccgaaaaacc
     8701 caggatcgac aacgtgtccg gcgccatttc ccggctgggg tatcgcacat tgcgcaattt
     8761 ggtgctgaac atcaccatgt cccgtctggc aaaagccgac cagcctgcaa tcaagatgct
     8821 gctcgccaat ttctgggaac acagccgcga agttgccgct atcagccata tgctggcaaa
     8881 aaaccagcgc cacctgaatg ccgatcaggc catgctggct ggcctgattc acgacatcgg
     8941 caccttgccg ctatgcatct atgtggacaa aatgcgttcg aatttcgacg gacctctgat
     9001 cgagggactg atcctgcagt ttcgtggtta tgtcggcgaa aaactgcttc gcacctgggg
     9061 cttccctgac gagatagtcg aggtgattcc cgaacatgag aatcttcagc gcaattcaga
     9121 atctcatcag gccagctatg ccgacatcgt caccgtagcc aacctgatga atcgactaac
     9181 cgccaaagtt gtcccctggg aagacatcac cgcgctgcaa cgactacata tcagcccgga
     9241 tatctgccga aactttcacg agcgtttcgg cgacgaacta agcacaacgc atgagatgct
     9301 gttcccgcat taagcagcgc gccgattgat gcggctctat tgcaagcgtt cggccgactg
     9361 caccaacacg tacggattca ccaccacggc ccacacatca gcatcagcct cgaaccaggc
     9421 tagcgcctgc tcgtccgtga ccttgccgac caagcctgcc accaaccatt gctcgacctg
     9481 agacctgttg tcttccgata tttgaaatgc cacatcgacc agatccagct ccgggcttac
     9541 tgccaccgca gccccgctgg caaagaagcg ctgcaactct ttccacgcaa tgcgcgaagt
     9601 ttcaaggttg atactggtac gattgatgtc ctgcaagctt gattctgtca tgctcaaatc
     9661 tctttctgat tacgatttca tccactcggg tgattatccc tcacccgaat atatgcctgg
     9721 tggaataaca cgggcaactg accttttcgg gacgtcccat taagcgcggg tattttaacg
     9781 gggcgggcga cttatcgcac cctttcccct cgcttaaaca atccgcaacc ctccatctaa
     9841 gcactatctg tctggatgct tttgatatac tcggcaatct tacgttttga gcctatatga
     9901 gcccgttgaa tatcgtcatc ctcgctgccg gaaaaggcac gcgcatgtat tccgacaagc
     9961 ccaaggtatt gcacacactg gctggcaaac cgctggtgca gcatgttctg gattgcgcca
    10021 cggcattgca gccgcaatct gtctgcgtga tctacgggca cggcggcgaa gccgtgccgc
    10081 aggcgatgag cccttatcag gcaaagttcg tgattcagga gccgcaactc ggcacggggc
    10141 acgccgtaca acaggcgatg ccccatctcc cggacggaag ccgcacgctg gtgctgtatg
    10201 gcgatgtgcc cctgactcag cacagcacgc tgcaccagat gctacaagcg ggcgagggcc
    10261 tgacgttact gaccgtgacg ctggataacc cgactggcta tggtcgcatc gtgcgcgatg
    10321 ctcagggtga tgtgcaatgc atcgtcgaag aaaaagacgc cacggcagag cagcgcgaaa
    10381 tacgcgaagt gaataccggc atcctgctgg cgccaaccgg gcaattgcgc gaatggctag
    10441 ccaggctggg caacaacaat gcacagggcg agtattacct gaccgacatt gtcggcatgg
    10501 ccgtgcggca gggtgtcgcg gttcataccg tgcagcccgc gcaacattgg gaagtcgaag
    10561 gcattaacag caaggcgcaa cttgcccagc tggagcgcac ctggcaacgg gtcgaagccg
    10621 gtcgcctgct ggcgcatggg gtcacgctgg ccgatccggc ccgtctcgat gtgcgcggca
    10681 agctcagctg cggacgcgat gtcgagatcg atgtcggttg catcttcgag ggcgacgtca
    10741 cactcgggga tggtgtgcga gtgggggctt acagcattgt ccgcaatgcg agcgtggcat
    10801 ctggcacaga cattgcgccc tacagccata tcgacagcag cgaagtgggc gcaaactgcc
    10861 acatcggccc ctatgcgcgc ctgcgtcccg gcaccagact ggccgatggc gcgcatatcg
    10921 gcaacttcgt cgaaatcaag aacagcgaaa tcgggcaggg cagcaaggcc aaccatctga
    10981 gctacatcgg cgacagtacc gtcggcagcc gcgtcaacat cggcgcgggc accatcacct
    11041 gcaattatga cggcgcgaac aagcatcgca ccgtgatcgg cgacgacgcc ttcatcggtt
    11101 cggatacgca actggttgca ccggtaacgg taggcaaggg ggcgactatc ggtgccggtt
    11161 cgaccatcac acgcgacaca ccggatcagg aattgacgct gtcgcgcagc aagcaactca
    11221 ccatcaccgg ctggaaacgg ccgcaaaaag gaaaaaaata gcatgtgtgg aatcgtcgga
    11281 gccgtcgcgc aacgcaatgt ggtacccatt ctgctggagg gactgaaacg gctggaatat
    11341 cgcggctacg actctgccgg cctcgccgtc gttcatgaag gccagttgca gcgcgtgcgc
    11401 agcaccggcc gggtcgctga gctgaccgca aaaagcgcca caacggcggg acaactcggc
    11461 atcgcccata cccgctgggc gacacacggc gttcccagcg agcgcaatgc ccacccgcac
    11521 ctgagcaacg accacatcgc cgtggttcac aacggcatca tcgaaaacta tgaagagctc
    11581 cgtaaacgcc tgaccgcgtc cgggtatgta ttcacttcgg acaccgacac cgaagtcatt
    11641 gcccatctga ttcacagtca ttacgtgctc ggcctgtcgc tgctggcagc gacccaggct
    11701 tcactggttg agttggcagg agcttacgcc atcggcgtga tcgcctccga tgaaccggac
    11761 cgattgatct gtgcgcggcg cggcagcccc ttgctgctcg gcgtgggtat cggcgaacac
    11821 tttatcgcct cggacgtgtc tgcgttgtta ccggtaaccc gcgaggtcgt ctacctggaa
    11881 gaaggcgacg tggtcgaact ggagcgtggc ggctaccgca tcttcgatgc gtcgggcacg
    11941 ctcaccacac gtccggttca gaccagcgaa cagagcaacg aaagcgtcga gctcggccag
    12001 taccgccact acatgcagaa agagatccac gagcagccgc aggccttggc cgacacactg
    12061 gagtcagtct gcaacagcca gtccttggtg ccgggcatct tcggcgcgga ggcggcggcg
    12121 accttcgacg acatcgacag catcctgatt ctggcctgcg gcaccagcaa ccacgcaggg
    12181 caggtggcac gctactggct ggaagaaatc gccggcattc cttgctccgt ggaaatcgcc
    12241 agcgaatacc gctatcgcgt cagcgtcccc aatccgaagg cactggtggt ggtgatctcc
    12301 cagtccggcg aaactgccga tactcaggcc gcactgaacc atgccaaggc gcagggccat
    12361 gtgcatacgc tggccatctg caacgtaccg gagagcgcgc tggtacgcct gtccaagctg
    12421 cgcctgctga cccgcgccgg ccccgagatc ggcgtcgcat cgaccaaggc gttcaccacc
    12481 cagctcgctg cgctattcct gctgaccctg gtgctggcca agcagcgcgg ccgcctgacc
    12541 cagcagcagg agagccaata cctgcacgag ttgcgtcatc tgcccagcgc ggtgcaaaag
    12601 gtgctggacc tggaaccgca gatcgccaag ctggccgagc gtttcgccga caagcacaac
    12661 gcgctgtttc tcgggcgcgg catgcatttc ccgatcgcgc tggaaggcgc gctcaagctc
    12721 aaggagatct cctacatcca cgccgaggct tatccggcag gcgagttgaa gcacggcccg
    12781 ctggcactgg tggataaaga catgccggtg atttcggtcg caccaaacga tgcgctgctg
    12841 gaaaaactga aatccaacct gcaggaagtg cgcgcccgcg gcggagaact gtacgtattc
    12901 gccgatgccg acacccaaat ccgcgaagca gatggcatcc acgtcatgca aatgcccgaa
    12961 cacgcagggt atttgagccc tattctgcac accatcccgc tgcaactgct ggcatattac
    13021 gtcgcactgc aaaagggaac ggatgtggac aagccgagaa acctcgcaaa
//
LOCUS       DASUIV010000145         3963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1244648, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000145 DASUIV010000000
VERSION     DASUIV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244648"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..776)
                     /locus_tag="VFW59_05455"
     CDS             complement(36..776)
                     /locus_tag="VFW59_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEX5363693.1"
                     /translation="MDMLAVLGLLVGVTGVLSGQLLEGGDLSILFQGAAFLIVLGGTL
                     GAVMVQSPRKVFFDAISMARWVFIPPNFTVQDTIRQITFWSSVARKDGVLALEPHVTE
                     ITDPFFIKGLHLLVDGHSAEKIREILEVDIHSWEDMRWSAARVWESAAGYSPTIGIIG
                     AVLGLMHVMQNLTEPSKLGSGIAVAFVATIYGVAFANLLFLPMANKLKFIVMQQTRMR
                     EMLVDGLVAIANGDNPRLVEARLQSYLP"
     gene            complement(776..1486)
                     /locus_tag="VFW59_05460"
     CDS             complement(776..1486)
                     /locus_tag="VFW59_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEX5363694.1"
                     /translation="MYNVSGLKDKDQCLREYAPLVKRLAHQMMTKLPYSVQIDDIIQA
                     GMMGLLDAASRYDEFHGAQFETYATQRIRGAMLDELREADWLPRGLRRDMRRIELAVS
                     RLQQKLGRAPNETEIAGELEVPLAEYQQMLQEARGAQLVYYEDFHEEGEEDFFERFEF
                     NSDANPLDLLQDERFKTELVRAIENLPERERMLMGMIYEQDMNLREIGAVFGVGESRV
                     SQLHSQAVARLRGWMKGY"
     gene            complement(1492..2385)
                     /locus_tag="VFW59_05465"
     CDS             complement(1492..2385)
                     /locus_tag="VFW59_05465"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX5363695.1"
                     /translation="MRSSAEGDQAEGLRRLLLRNQTQVITVVSGKSGVGRTSATINFA
                     AALVRSGKDVLVLDENPVPHNLLDRLGLSARYDLLDVIQGKCSPGEAVLSSKGVSVLP
                     TARAMLALATLNQAEQQRLENALNVISSGVDVMLVDAAMLVGQAAVSSSLASGASLLV
                     VVDGTAAGITESYALIKRLALENARLRFEIVVNRVDNEQAAITVFNNMAKVAQRHLAA
                     RLEYLGHIPFDDRLKRSTQLGRSVVEAFPASDSAKSYLELSQKLLRLPVQQEEAEGGI
                     SRMMKSLILQLRSGEMAHVVN"
     gene            complement(2375..3661)
                     /gene="flhF"
                     /locus_tag="VFW59_05470"
     CDS             complement(2375..3661)
                     /gene="flhF"
                     /locus_tag="VFW59_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEX5363696.1"
                     /translation="MNIRKFIAPTAREALRDIRNELGNEAVILSNRRTASGVEIIALA
                     NEDVVRMTDKAVDQPLASRTPMPPPMKPVASQTQPTPLAAPVSEMKAAVQAPPVAAQT
                     PPVAEAAPTPSPAVHENILGEIKSMHLMLKQLAAMSWNDVQHRDPQRAGMVRRMLNSG
                     FSALLARQLMDKMPAGKTQGESWVKQVLKRNLRVAGEADDIIAKGGVYALIGPTGVGK
                     TTTTAKLAARAVVRYGADKVALLTTDSYRIGAYEQLKIYGKILGVAVHAVKDTDDLRL
                     TLSALRHKHLVLIDTVGMGQRDERVGDQNEMFNATGVQRLLMLNATSGGDTLEDVVRM
                     YHSDRVIGCIATKLDETVNLGAVLDVVVRHRLVMHFMTNGQRVPEDLFPVNADYLLHR
                     ALKPAEEKTPFTLHEQEFPLLMAGAQTQIKEAAYAL"
     gene            complement(3658..>3963)
                     /locus_tag="VFW59_05475"
     CDS             complement(3658..>3963)
                     /locus_tag="VFW59_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEX5363697.1"
                     /translation="ELQVIGMDKELEYILTQAMQSSQSGIGIEPGLADTVLRETRAAA
                     EAQEQMGLPAVMLVPSQLRDLLARFLKRAVPSIRVISHDEVPDFKTIRVTAMVGGRA"
BASE COUNT          869 a         1295 c          956 g          843 t
ORIGIN      
        1 atcgtgctcg tggcgttttc ttctgcgtgc catgatcagg gcaaatagct ttgcaagcga
       61 gcctctacca agcgaggatt atcgccgttg gcgatcgcca ccaacccatc caccagcatc
      121 tcccgcatac gcgtctgctg catgacaatg aatttaagct tgttcgccat cggtagaaac
      181 agcaggttgg caaaagccac cccgtaaatc gtcgcgacga atgccaccgc aataccgctg
      241 cccaatttgc tcggctctgt caggttctgc atgacatgca tcaagcccaa gacagccccg
      301 ataattccga tagtaggcga ataacctgcg gcagactccc atacgcgcgc agccgaccag
      361 cgcatatcct cccacgaatg aatatcgacc tccaggattt cgcgaatttt ttccgcacta
      421 tgaccatcta ccagaaggtg cagacccttg atgaaaaacg gatcggtgat ttcggtaaca
      481 tgaggttcca gcgccagcac accatccttg cgcgccaccg aactccagaa agtaatctgc
      541 cggatcgtat cctgtaccgt gaaattgggc ggaataaaca cccagcgcgc catgctgata
      601 gcatcgaaaa aaaccttcct cgggctttgc accatcaccg ccccgagcgt tcctcccagc
      661 acgatcaaaa aggctgcacc ttgaaacaga atggaaagat cccctccctc cagcaactgt
      721 cccgacaaaa ctccggttac tccaaccagc aatcccaaca ccgcgagcat atccatcaat
      781 agcctttcat ccagccgcgc agtcttgcca ctgcctgact gtgtaactgg gaaacacgcg
      841 actcaccgac cccaaatacg gccccgattt cgcgcaaatt catatcctgc tcgtaaatca
      901 tccccatcaa cattcgctcg cgctccggca gattttcaat tgcccgaacc aactcggttt
      961 taaaacgctc gtcctgcaat agatccaagg gattggcatc actattgaat tcgaagcgtt
     1021 cgaaaaaatc ttcctcgccc tcttcgtgaa aatcctcgta gtacaccagt tgcgcaccgc
     1081 gcgcctcctg aagcatctgc tgatactcgg ccagaggcac ctccagttcg cccgcgattt
     1141 ccgtttcatt cggagcccgc cccagcttct gctgcaaacg gctgacggcc aactcgatgc
     1201 gacgcatatc gcggcgcaaa ccgcgcggca accaatccgc ttcgcgcaac tcatccagca
     1261 tcgcaccgcg aatgcgttgc gtcgcgtagg tttcgaactg cgcgccgtga aattcgtcgt
     1321 agcggctggc agcgtccagc aaccccatca tgccagcctg aatgatgtcg tcaatttgta
     1381 cgctgtacgg cagctttgtc atcatctgat gagcaagccg ctttaccagc ggcgcatact
     1441 cccgcagaca ctggtctttg tcttttagtc ctgatacgtt atacatgccc gctagtttac
     1501 aacgtgtgcc atttctccgc tgcgcaactg caatatcaag ctcttcatca tgcgggaaat
     1561 gccgccctct gcttcctctt gctgtacagg cagacgcaac aacttctgcg ataactccag
     1621 atacgatttc gccgaatcgg acgccggaaa tgcctcaaca acagaccggc ccaactgggt
     1681 cgaacgcttc aagcgatcat cgaaaggaat gtggcccaaa tactccagac gcgcggccag
     1741 atgacgctgc gccaccttgg ccatattgtt gaacacggtg atggctgcct gttcgttatc
     1801 gactcgattc accacgatct cgaagcgcaa acgagcattc tccagcgcca gacgcttgat
     1861 caatgcatag ctttcggtaa taccggccgc cgttccgtcc accactacca gcaggctagc
     1921 gcccgaagca agactcgaag acacggctgc ctgaccgacc agcattgccg catcaaccag
     1981 catcacatcg acaccgctgc taatcacgtt cagcgcattt tccagccgct gctgttcagc
     2041 ctgattcagc gtcgccagcg cgagcatcgc gcgtgcggtc ggcaacacgc taactccctt
     2101 gctgctcagt acggcctcgc cgggactgca cttcccctga atgacatcca gcaaatcata
     2161 ccgcgcggat agccccagac gatccagcaa attatggggc acaggatttt catccagcac
     2221 cagcacatct ttgccggagc gcaccagcgc cgcagcaaaa ttgatggtcg cactggtgcg
     2281 acccacaccg ctttttcccg acacgaccgt gattacctgc gtttgattac gcagcaacaa
     2341 acggcgcaat ccttcggctt gatccccttc agcactagag cgcatatgcg gcctccttga
     2401 tctgggtctg agcgcctgcc atcagcagcg gaaattcctg ttcgtgcaac gtaaacggcg
     2461 ttttttcttc agccggttta agcgcgcggt gcaacaaata atcggcattg accgggaaca
     2521 ggtcttccgg cacgcgctga ccgttggtca taaagtgcat caccaggcga tggcgcacca
     2581 cgacatccag aaccgcaccg agattaaccg tctcatcgag tttggtcgcg atgcaaccga
     2641 tcaccctgtc gctgtgatac atccgcacca catcttccag cgtatcgccg ccgctagtgg
     2701 cattcagcat cagcaagcgc tgcacgcccg tcgcattgaa catctcgttc tgatcgccga
     2761 cacgctcgtc acgctgtccc atccccaccg tgtcgatcag caccagatgt ttgtggcgca
     2821 gtgcggaaag ggtcagtcgc aaatcgtcgg tatctttcac cgcatgcaca gcaacaccga
     2881 ggatcttgcc gtaaattttg agctgctcgt aagcgccgat acgatagcta tcggtcgtca
     2941 gcagcgccac cttatccgcg ccgtaacgca ccaccgcgcg tgccgcgagc tttgctgtcg
     3001 tcgtcgtctt gcccaccccg gtcggcccga tcagcgcata tacgccgccc ttggcaataa
     3061 tgtcatccgc ctcgccggcc acacgcaagt tgcgcttcaa cacctgcttg acccatgact
     3121 cgccctgcgt ttttccggcg ggcatcttgt ccatcaactg gcgtgccagc aatgcactga
     3181 atccggagtt gagcatcctg cgcaccatcc cggcacgttg cggatcgcgg tgctgcacgt
     3241 cgttccagga cattgccgcg agctgcttca gcatgaggtg catggacttg atctcgccca
     3301 ggatattctc atgtaccgcc ggagacggcg ttggcgcagc ctcggcaaca ggaggagtct
     3361 gagcggcaac aggaggtgcc tgaactgcgg ccttcatttc ggatacaggc gcggccaacg
     3421 gggttggctg agtctgggat gcgaccggct tcatcggcgg cggcatcgga gtccgggatg
     3481 ccagcggctg atcgaccgcc ttgtcggtca tgcgtacaac atcctcattc gccagggcaa
     3541 taatctccac gccgctcgcc gtcctgcgat tggacaggat caccgcctca ttgcccagtt
     3601 cgttgcgaat gtcccttaag gcctctcggg ctgtcggggc tataaatttt ctgatgttca
     3661 tgctctacct cccaccattg cagtcacccg gatagttttg aagtccggta cttcatcgtg
     3721 cgaaatcacc ctgatacttg gcactgcacg cttcaggaaa cgcgccagca aatcgcgcaa
     3781 ttgcgacgga accagcatca ctgccggcaa ccccatttgt tcctgagcct cggctgcggc
     3841 gcgagtttcg cgcaacacgg tatccgccaa ccccggctcg atgccgatgc cgctctggct
     3901 ggactgcatg gcctgagtca ggatgtattc gagctccttg tccatgccga tcacctgcaa
     3961 ctc
//
LOCUS       DASUIV010000146         4906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1058194, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000146 DASUIV010000000
VERSION     DASUIV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058194"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1342)
                     /gene="rlmM"
                     /locus_tag="VFW59_05480"
     CDS             complement(266..1342)
                     /gene="rlmM"
                     /locus_tag="VFW59_05480"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HEX5363698.1"
                     /translation="MNKPSSTLWLLYCRPGFEQDCAQEALRQARQQKPVIADQPPITA
                     DSGYVMIALNDQRLSYRELVFTRQLIQLQYIIEELPERDRLTPIVEAIAALPDTFGTL
                     WLEVPDTNDGKTLSAFTRRFQPLLEEALRAQGKLPPESPEINRTQPRLHLFFPDKSTV
                     LIGSSDPYNGAPWHMGISRQHMPSEAPSRSTLKLAEAIEVFMDKSEQTRLMRQGMTAV
                     DLGAAPGGWTWQLVKRGIRVTAVDNGPMKGVLAKHPLVEHLRQDGFKYAPRKAVDWLV
                     CDMVEKPGKVATLIGSWFVAGFAKHAIFNLKLPMKQRLTAVDSALNDIRKQLDDEGIN
                     YRMSAKQLYHDREEVTVFLTRTKG"
     gene            complement(1361..1972)
                     /locus_tag="VFW59_05485"
     CDS             complement(1361..1972)
                     /locus_tag="VFW59_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX5363699.1"
                     /translation="MNLIYLLDLCAVAVCAITATLEARRRELDLFGVIVIAVISGIGG
                     GTVRDLLLGRLPVYWVHDPIYVVVGIAAGTLTFFLGRRLQLPKNFFLVPDAVGLALFT
                     VIGTSVALQLQVPWLIAALMGVITGVFGGVIRDILCNEVPLIFRTDIYATASLAGALL
                     LIALDDLGAGHNLAILLAMIGTVAIRLAAIRWHIHLPRLHSDV"
     gene            2087..2743
                     /locus_tag="VFW59_05490"
     CDS             2087..2743
                     /locus_tag="VFW59_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5363700.1"
                     /translation="MTKRYDLIVFDWDGTVMDSTAIIAGSIQAACRDLGLNVPSDEAA
                     RHVIGLGLAQALRHAVPDAPEEMYEPLVERYRYHFLSQDLAIPLFEGARETIAELHAD
                     GYRLAVATGKSRAGLDRVMDSSGMRGYFHATRTADQTFSKPHPAMLFELMEELAVSAE
                     RTLMIGDTTHDVQLAQNAGVDVVAVTYGAHPPEQLQLLEPLALVEDFNELRVWLKANA
                     "
     gene            2886..3830
                     /locus_tag="VFW59_05495"
     CDS             2886..3830
                     /locus_tag="VFW59_05495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX5363701.1"
                     /translation="MSEDNNWERGVLEKLAMSAVQEQRRARHWGIFFKVLTFGYLFLI
                     LFLIMGWMGAGESKLSTGKHTALVDMQGVIAPDTAAGADFVIPSLQDAFEDKGTQGVI
                     LRINSPGGSPVQAGQINDEIRRLRAMHPEIPLYVVVEDICASGGYYVAVAADKIFVDK
                     ASLIGSVGVLMDGFGFTGTMEKLGVERRLLTAGTNKGFLDPFSPSNPEQQEYAKQMLS
                     EIHQQFIEVVRQGRGKRLKETPDTFSGLVWNGQRGVEMGLADGYGSIESVARDVIKAE
                     KVVDFTMKEGFGERLAKRFGAGVASAIGFSAQGGVALR"
     gene            complement(3904..4494)
                     /locus_tag="VFW59_05500"
     CDS             complement(3904..4494)
                     /locus_tag="VFW59_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX5363702.1"
                     /translation="MKIILLIGALWLPCSTHAAQNVLVFGDSLSAGYGIQTEESWPVL
                     LQHELNESDYRVVNASISGETTQGGRQRIAQTLLRHRPAVVILELGANDGLRGYATTD
                     IEANLNKLIVDIKRSGASVLLVGMRLPPNYGKTYVSQFEELYPRLAKARSVRLLPFLL
                     EGVPPEQFQPDNLHPTAAAQPLIMRKVLKELRPLLD"
     gene            4508..>4906
                     /locus_tag="VFW59_05505"
     CDS             4508..>4906
                     /locus_tag="VFW59_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5363703.1"
                     /translation="MVPIVQAVELTRQVLSGDRPLTILHGVSFTVAAGESLAIIGSSG
                     SGKSTLLGLLAGLDVPSSGSVRLNGTDLYALDEDGRARLRGELAGFVFQSFQLLPTLS
                     ALENVMLPLELQGRTDAGERAAESLQQVGLA"
BASE COUNT          895 a         1418 c         1634 g          959 t
ORIGIN      
        1 atgactgcaa ttggcacgca acggccgtac accatgctaa cagcatgcca caaaccggtc
       61 attcatggtc aattcagatc agcctagtgc gacagtccgc aatggccgaa gggctgaagt
      121 tctgaatcac tttcccggat aaccgcttta ggtcgatggc cgacttaacc ttttcccgtt
      181 gccttgctga aacctgagcg tcggaacagc aattgagacg ctatgaaaaa aggcctccat
      241 ctctggaggc ctttgtgttt ttcgtctagc ccttggtgcg agtgaggaat accgtcactt
      301 cctcgcggtc gtgataaagc tgtttggcgc tcatccggta gttgatgcct tcatcgtcca
      361 gttgtttgcg gatgtcgttc agcgcgctgt ccactgcggt gaggcgttgc ttcatcggca
      421 gcttgaggtt gaagatggca tgtttggcga atccggcaac gaaccagctg ccgatcagcg
      481 tggcgacctt gccgggtttt tccaccatgt cgcacaccag ccagtccacc gccttgcgcg
      541 gcgcatattt gaaaccgtcc tgacgcagat gctcgaccag cgggtgcttg gccagcacgc
      601 ccttcatcgg gccgttgtcc actgcagtga cgcggatgcc gcgcttgacc agttgccagg
      661 tccagccgcc gggagcggca ccgaggtcga cggcggtcat gccctgtcgc atcagtcgcg
      721 tctgttcgct cttgtccatg aagacttcga tggcctcggc cagcttcagc gtcgagcggc
      781 tcggcgcttc cgatggcatg tgctggcgcg agatgcccat gtgccagggc gcgccgttgt
      841 agggatcgct gctgccgatc agtaccgtgg acttgtccgg gaaaaagaga tgcaggcgcg
      901 gctgtgtccg gttgatttcc gggctctcgg gcggcagctt gccctgcgcg cgcagcgctt
      961 cttccagcag cggctggaag cggcgggtga aggccgacag cgtcttgccg tcgttggtgt
     1021 ccggcacttc cagccacagc gtgccgaagg tatcgggcag cgctgcgatc gcctcgacga
     1081 tcggggtgag gcggtcgcgc tcgggcagct cttcgatgat gtattgcagc tggatcagct
     1141 ggcgcgtgaa gaccagctcg cggtaggaaa gccgctggtc gttgagcgcg atcatcacgt
     1201 agccgctgtc ggcggtgatg gggggctggt cggcgatgac cggcttttgc tggcgggcct
     1261 gacgcagcgc ttcctgcgcg cagtcctgtt cgaagccggg gcggcagtac agcagccaaa
     1321 gggtgctcga aggtttgttc atatgagcct ctctattaag ttaaacgtcc gaatgcaggc
     1381 gcggaaggtg gatatgccag cggatcgcgg cgaggcgtat cgccaccgtg ccgatcatcg
     1441 cgagcaggat ggcaagatta tggcccgcgc ccagatcgtc gagggcgatc agcagcaggg
     1501 cgcccgccag cgaggcggtg gcgtagatgt cggtgcggaa aatcagcggg acttcgttgc
     1561 acagaatgtc gcggatcacg ccgccgaata cgccggtgat cacgcccatc agcgcggcga
     1621 tcagccaggg tacctggagc tggagcgcga cgctggtgcc gatcacggtg aacagcgcca
     1681 gccccaccgc atcggggacg aggaagaagt tcttcggcag ctgcaggcga cggcccagaa
     1741 agaaggtcag cgtgcctgcc gcgatgccca cgacgacata gatcgggtcg tgtacccaat
     1801 acaccggcag gcgccccagc agcaggtcgc gcaccgtgcc gccgccgatg ccggagatca
     1861 ccgcgatcac gatcacgcca aacaggtcca gctcgcggcg gcgagcttcc agcgtggcgg
     1921 tgatcgcgca gacggcaacc gcgcaaaggt cgagcagata gatcagattc atgatgcagg
     1981 ctatggggca gaggcgcgca ttatacgagc cggggcgttt cttcgcgtgc gctattcggc
     2041 tgggggtggg tggcgtggta agattcgcga cttttggcgg tggggtatga cgaagcgtta
     2101 cgatttgatc gtgttcgact gggacggcac ggtgatggac tcgacggcga tcatcgccgg
     2161 ctcgattcag gccgcgtgcc gcgacctcgg gctgaacgtg cccagcgacg aggcggcgcg
     2221 tcatgtgatc gggctgggac tggcgcaggc cttgcgccac gcggtgccgg atgcgccgga
     2281 ggagatgtac gagccgctgg tcgagcgcta ccgctaccat ttcctgtctc aggacctggc
     2341 cattccgctg ttcgagggcg cacgcgagac catcgccgag ctgcatgcgg acggttaccg
     2401 gttggctgtc gccaccggca agagccgcgc gggactggat cgggtgatgg actccagcgg
     2461 catgaggggg tatttccacg ccacgcgcac cgccgaccag actttctcca agccgcatcc
     2521 ggcgatgctg tttgaactga tggaagaact ggcggtcagt gcagagcgca cgctgatgat
     2581 cggcgacacc acgcacgatg tgcagctggc gcagaatgcc ggcgtggatg tggtggcggt
     2641 gacttacggc gctcatccgc cggagcaatt gcagctgctg gagccgcttg cgctggtgga
     2701 agattttaat gaactgcgcg tctggctgaa ggccaacgct taacgtgaaa ttcgcgcagc
     2761 gactcgttgg ccctttctcc cgcttgcggg ggaaagttgg atagggggaa acgggcgtgg
     2821 caagtttcat catcaggggt ggcgtacttg ccatgcccgt taatgatttt tgagaaggaa
     2881 tagcaatgtc tgaagacaac aactgggaac gcggtgtact ggaaaaactg gcgatgtccg
     2941 ccgttcagga gcagcgccgt gcgcgccatt ggggcatctt cttcaaggtg ctgactttcg
     3001 gttatctgtt cctgatcctg ttcctgatca tgggctggat gggcgccggc gagtccaagc
     3061 tgagcaccgg caagcacacc gcgctggtgg acatgcaggg cgtgatcgcg ccggatacgg
     3121 cggcgggtgc cgatttcgtc atccccagtc tgcaggatgc cttcgaggac aagggcacgc
     3181 agggtgtgat tctgcgcatc aacagcccgg gtggcagtcc ggtgcaggcc ggccagatca
     3241 acgacgagat ccgtcgcctg cgcgcgatgc atccggagat cccgctgtac gtggtggtcg
     3301 aggatatctg cgcttccggc ggatactacg tggcggtcgc ggcggacaag atcttcgtgg
     3361 acaaggccag cctgatcggt tccgtcggcg tgctgatgga cggcttcggc ttcaccggaa
     3421 cgatggagaa gctgggcgtg gagcgccgtc tgctgaccgc aggaacgaac aagggtttcc
     3481 tcgatccgtt ctcgccctct aatcccgagc agcaggaata cgccaaacag atgctgtccg
     3541 agattcacca gcagttcatc gaagtcgtgc gtcagggtcg cggcaagcgt ctgaaagaaa
     3601 cgccggacac cttcagcggc ctggtctgga acggtcagcg cggcgtggag atgggcctgg
     3661 ccgacggtta cggcagcatc gaatcggtgg cgcgcgatgt gatcaaggcc gagaaagtgg
     3721 tggacttcac catgaaggaa ggcttcggag aacgtctggc caagcgcttc ggcgcagggg
     3781 tggcgagtgc catcggcttc tcggcacagg gcggcgtggc actgcgctga ccggcttgcc
     3841 gtgcaataga aaaaggctca tttccccttg gggaaatgag ccttttttgg tctggcgagc
     3901 cgctcaatcc agcaacggcc gcagttcctt cagcaccttc cgcatgatca gcggttgcgc
     3961 ggccgcagtc ggatgcaggt tgtccggctg gaactgttcc ggcggcacgc cttccagcag
     4021 gaagggcagc aaccggacgc tgcgcgcctt cgccagacgg gggtagagtt cctcgaactg
     4081 gctgacgtag gtcttgccgt aattgggcgg caggcgcatg cccaccagca gcacgctggc
     4141 gccgctgcgc ttgatgtcta cgatcagttt gttcagattg gcttcgatgt ccgtcgtggc
     4201 atagccgcgc aacccgtcgt tcgcacccag ttccagtatc accaccgccg gacgatgccg
     4261 cagcagggtc tgggcgatgc gctgccgtcc cccctgcgtc gtctcgccgc tgatgctagc
     4321 attgaccacg cggtaatccg attcgttgag ctcatgctgc agcagcaccg gccaactttc
     4381 ttctgtttga atgccgtaac cggctgatag actgtcgccg aacaccagaa cgttctgcgc
     4441 ggcatgggtg gagcagggca gccacagcgc gccgattaat aagataattt tcaaaggtag
     4501 ggtcttcatg gttccgattg tgcaggcagt tgaactgact aggcaagtgt tgagcggcga
     4561 tcggccgctg acgatactgc acggggtcag tttcaccgtg gcggcaggcg aaagtctcgc
     4621 gatcatcggc agttccggtt ccggcaagtc tacgctgctc ggcctgctcg ccgggctgga
     4681 tgtgccttcc agtggcagcg tgaggctgaa cggcacagac ctgtacgcac tggacgagga
     4741 cggacgtgcc agactgcgcg gcgaactggc gggcttcgtg ttccagtcgt tccagctctt
     4801 gcccacactc tccgcactgg agaacgtcat gctgccgctg gagctgcaag gccgcaccga
     4861 tgcgggtgaa cgcgccgccg aatcgctgca gcaagtcggc cttgcc
//
LOCUS       DASUIV010000147         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_779157, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000147 DASUIV010000000
VERSION     DASUIV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779157"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VFW59_05510"
     CDS             <1..1015
                     /locus_tag="VFW59_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5363704.1"
                     /translation="EGLKAGEIDLIQENSSKNWARNHQGKRWEDGTLVKALLPHQNGA
                     GWQGFAMNTRRPLFQDKRVRQALGLAMDFEWMNRQLFFNLYQRSPSYFTNTELAATGK
                     PDARELTVLHELQTRFGDKVPAEIFDDIPPPPSTLPPHSLRDNPRQARELLAQAGWNS
                     RDGALRNAQGEPFQFEMMLEDRMQERASAPYARNLEKLGIAMTYRIYDAALLQRKAEN
                     FDYDMVWALMSGSQSPGNELFGYFASSSRDQPGSDNAMGVADPVIDALLARIVQAEKR
                     DELVTLVRVLDRMLRLGYYIVPHFYSNAHRVSYRHTLAFPQVLPQFYRIEDWSVTTWW
                     MKP"
     gene            1098..2153
                     /gene="yejB"
                     /locus_tag="VFW59_05515"
     CDS             1098..2153
                     /gene="yejB"
                     /locus_tag="VFW59_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HEX5363705.1"
                     /translation="MMHYVGKRLLLMIPTLLGILLLTFTVIQFVPGGPVEQMVSELRG
                     KGHGSQEAGGGGQSIYRGQQGIDAERMAEIRVLYGFDKPAPERFWLMLKGYATLDLGT
                     SFFHQKSVWELVKEKMPVSVSLGLWTLIITYLVSIPVGIAKAVRDGSRFDTATSFAIL
                     SGYAIPGFVLGVLLLVLFGGGSFWQWFPLRGLTSDNWAQLSPVAKVTDYLWHITLPVF
                     AQVVGSFAVVTMLTKNTFIEEIRKQYVLTARAKGLPERTVLYRHIFRNALLPLLTGFP
                     AAFIGAFFTGSLLIETLFSLDGLGLLSYNAIMQRDYPVVMGTLFIFTLAGLFAKLISD
                     VSYVLADPRVKFEGVAQ"
     gene            2159..>3165
                     /locus_tag="VFW59_05520"
     CDS             2159..>3165
                     /locus_tag="VFW59_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5363706.1"
                     /translation="MAEDIKPVLPSLSPNQQRWRRFRRNRLGYASLLIFVVLFVLSLF
                     AEVLSNDRPLIASYQGTLYFPLWHDYPESTFGGDFPTPADYLDPFIREQFARPGNWAV
                     YPPNPYRYDTLNYFSTQPNPAAPSAENWLGTDDRGRDVLARAFYGFRVSVLFGMALTV
                     IGVLLGVLAGAIQGYFGGRIDLYLQRFSEIWGSLPELYLLLIFASFFQPSITLLLILL
                     SLFGWLALADYVRAEFLRNRQLDYVRAARALGLGNRQIMWRHILPNSLTPVITFLPFR
                     MSVSITALTSLDFLGLGVPPSTASLGELLAQGKDNLDAWWIGLAAFVVLFVTLLLLVF
                     IG"
BASE COUNT          522 a          930 c         1030 g          683 t
ORIGIN      
        1 ggaagggctg aaggcggggg agatcgacct gattcaggag aattcctcga agaactgggc
       61 gcgcaatcat cagggcaagc gctgggaaga tgggacgctg gtcaaggcgc tgctgcctca
      121 ccagaacggc gcgggttggc agggctttgc gatgaatacg cgccgtccgc tgtttcagga
      181 caagcgggta cgtcaggcgc tggggctggc gatggacttc gaatggatga accgtcagct
      241 gttcttcaac ctgtaccagc gcagccccag ctatttcacc aacacagaac tggccgcgac
      301 aggcaagcct gacgcgcgtg agctgacggt gttgcatgaa ttgcagacgc gttttggcga
      361 caaggttccg gcagagatat tcgatgacat tccgccgcca ccgagcacgc tgccaccgca
      421 ttccttgcgc gacaacccgc gtcaggcgcg cgagttgctg gcgcaggcgg gctggaactc
      481 tcgcgacggc gcgttgcgca acgcgcaggg tgagccgttc cagttcgaga tgatgctgga
      541 ggaccgcatg caggagcggg catcggcgcc ctatgcgcgc aatctggaaa agctcggtat
      601 tgcgatgact taccgtattt acgacgccgc cttgctgcag cgcaaggccg agaacttcga
      661 ttacgacatg gtgtgggcgc tgatgtccgg ctcgcaaagc ccgggcaacg agttgttcgg
      721 ctatttcgcg tcgagcagcc gcgaccagcc cggctccgac aatgcgatgg gtgtggctga
      781 tccggtgatc gacgcgctgc tggcgcgcat cgtacaggca gaaaagcgcg acgagctggt
      841 gacgctggtg cgcgtgctcg accgtatgct gcgtctgggt tattacatcg tgccgcattt
      901 ctacagcaac gcgcaccggg tttcctaccg tcatacgctg gcgtttccgc aagtgctgcc
      961 ccagttctat cgcatcgagg actggtcggt gacgacctgg tggatgaagc catgaatgta
     1021 ataatcgcgc agcgaatctt tgtccctctc gcccgcttgc gggagagagc cggagagagg
     1081 gaagaggaga gccgaccatg atgcattacg tgggcaaacg tctgctgctg atgatcccca
     1141 ccctgctggg catcctgctg ctgaccttca cggtgatcca gttcgtgccc ggcgggccgg
     1201 tcgagcagat ggtcagcgag ttgcgaggca agggccacgg cagccaggag gcgggcggcg
     1261 gcgggcagag tatctatcgc gggcagcagg ggatcgatgc cgagcgcatg gccgagatca
     1321 gggtgctgta cggtttcgac aagccggcgc cggagcgttt ctggctgatg ctcaagggct
     1381 acgctacgct cgatctgggc accagcttct ttcaccagaa atcggtgtgg gagctggtca
     1441 aggagaagat gccggtgtcg gtctcgctgg gcctgtggac gctgatcatc acctatctgg
     1501 tgtctatacc ggtgggtatc gccaaggcgg tgcgcgacgg ttcgcgtttc gataccgcga
     1561 ccagtttcgc gatcctgagc ggttatgcga ttcccggctt cgtgctgggc gtgctgctgc
     1621 tggtgctgtt cggcggcggc agcttctggc aatggttccc gttgcgcggg ctgacgtcgg
     1681 acaattgggc gcagctctcg ccggttgcca aggtgaccga ttatctgtgg cacatcacgc
     1741 tgccggtgtt cgcgcaggtg gtcggcagct tcgcggtggt cacgatgctg accaagaaca
     1801 ccttcatcga agagatccgc aaacaatacg tgctcaccgc gcgcgccaag ggcttgccgg
     1861 agcgcacagt gctgtaccgg catatcttcc gcaatgcctt gctgcccttg ctgactggtt
     1921 ttccggcggc cttcatcggc gcgttcttca ccggcagcct gctgatcgag acgctgttct
     1981 cgctcgacgg gctggggctg ttgtcctata acgcgatcat gcagcgcgat tatccggtgg
     2041 tgatggggac gctgttcatc ttcacgctcg cggggctgtt cgccaagctg atctcggatg
     2101 tcagttatgt gctcgccgat ccgcgcgtga agttcgaagg ggtggcgcag tgagcgagat
     2161 ggcggaagac atcaagcccg tgctgccgtc gctgtcgccc aaccagcagc gctggcggcg
     2221 ctttcgccgg aaccgtctgg gttatgccag cctgctgatc ttcgtcgtgt tgttcgtgct
     2281 cagtctgttc gccgaggtgc tttccaacga ccgtccgctg atcgcctctt accaggggac
     2341 gctgtatttt ccgctatggc acgattatcc agaaagcacg ttcggcggcg attttccgac
     2401 tcccgccgat tatctcgacc ccttcatccg cgagcagttt gcgcggcccg gcaactgggc
     2461 ggtctatccg ccgaatcctt accgctacga cacgctgaat tatttttcga cacagcccaa
     2521 tccggcggca cccagtgcgg agaactggct gggcaccgac gatcgggggc gcgacgtgct
     2581 ggcgcgtgcc ttctacggtt ttcgcgtgtc ggtgctgttc ggcatggcgc tgacggtcat
     2641 cggcgtattg ctgggggtgc tggcgggcgc gatacagggc tacttcggcg ggcgcatcga
     2701 tctttatctg caacgctttt ccgaaatctg gggttcgttg cccgaactgt atctgctgct
     2761 gatcttcgct tccttctttc agcccagcat cacgctgctg ctgatcctgc tgtcgctgtt
     2821 cggctggctg gcgctggccg attatgtgcg cgccgagttc ctgcgcaatc gccagctcga
     2881 ctacgtccgc gcggcgcgcg cgctggggct gggcaaccgc cagatcatgt ggcggcatat
     2941 cctgcccaac agcctgaccc cggtgatcac cttcctgccc ttccgcatga gcgtgtccat
     3001 taccgcgctg accagtctgg acttcctcgg tctcggtgtg ccgccttcga cggccagtct
     3061 gggcgagttg ctggcacagg gcaaggataa cctcgatgcc tggtggatag gtctcgccgc
     3121 attcgtcgtg ctgttcgtca cgctgctgtt gctggtgttc atcgg
//
LOCUS       DASUIV010000148        11627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_871316, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000148 DASUIV010000000
VERSION     DASUIV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871316"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /gene="arsC"
                     /locus_tag="VFW59_05525"
     CDS             <1..351
                     /gene="arsC"
                     /locus_tag="VFW59_05525"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX5363707.1"
                     /translation="MSVSMYHNPKCSKCRATLTLLQEKGIEPELILYLETAPSAAALE
                     EVLKKLDKGPKDIIRFNEEVAKELGISPADERPDPEWLQIISGNPKLLERPIVINGDR
                     AAIGRPPERVLEIL"
     gene            complement(770..1420)
                     /locus_tag="VFW59_05530"
     CDS             complement(770..1420)
                     /locus_tag="VFW59_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5363708.1"
                     /translation="MRIRLMLAHEHDLLRAGIVQLFHSDPDIEVVAEVSCSSGLLNKL
                     QTFYVDVLLLGMPMPAVYEGELIARIKGAHPDLPVLVLGMHDDVMSVMRAMKAGASGF
                     INTHCAPQTLIGAIRKVATTGRYLDPKMAEQLVYARNSTALAKMESILSDRELQILPL
                     IAEGKCIKSIANQLAISDKTVSTHKAHILCKLGLNNVADLVRYVIENESLSRIPVR"
     gene            complement(1594..2211)
                     /locus_tag="VFW59_05535"
     CDS             complement(1594..2211)
                     /locus_tag="VFW59_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5363709.1"
                     /translation="MLVDDHKIFRDALRSLLEKMPNLEVVGECGNPFEVIDLARKSSA
                     KIVCMDIGIPKMNGIEITRNLLAACPNVKVIALSSYSDRRYVMDMMNAGASAYVTKAE
                     ATDELLRAIDALKHNRKYLCPDVVEAVTGAMSPHATLSSNLGNRERQVLQLVAEGLTS
                     AMIADQLNIAPSTVEVHRRNIMRKLNLHNVAELTRYAINNGLAIN"
     gene            complement(2271..4346)
                     /locus_tag="VFW59_05540"
     CDS             complement(2271..4346)
                     /locus_tag="VFW59_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5363710.1"
                     /translation="MALSVFIKNEGQTMRIDESLRQIAKVPERGENGEVIGALAIGRD
                     ITGRKQLELAIAVREQEFRSLAESSPDSIIRYDCAGRILYLNDRLLKQLELASAKEVI
                     GKRPGEVWSDGRFAELERAAARAADSGSQVDIELVVPTENGAFHYHQIFVVPERDVGG
                     QIVGTIAFGREITVIREAERRLKHFIDNMPGFAYTFRLSPDGHASFPFISPSIEKFYG
                     LKPEDVQDDIAPLHSFWHPDDRMHIEAATAESARTMTPFRIESRVCRPGQPERWLDAR
                     ALPEREADGSILWHGIMLDITERKQAEALLKERLCQIEELNSNLGESARNLRVQTEEL
                     AASQEHLKQTETWYRSILHSAPDGMVVTDARGVITQVNARIEAMFGYAKGELVGLPVE
                     RLLPPDVRENHVDKRTDFAVSTTNSLKSVSARGLRGCRKDGTEFPVDVSLSRLPDTDE
                     KVGHVCAAIRDITERKKMERERINHLMRLEEISRNLVAAQEDARQRLACELHDRTSPN
                     LAAIDINLNVIAAEIAQGTSTDLTERLEDIRALISDTSASIREIGAEMRPPLLDYAGL
                     IAAMENYAFLFARRTGIQIYFECEKRDTRYTPQIESLLFRILQEALTNCSKHSSAESI
                     RISLCNEIRPNILTITDDGVGFDPAQLGKTRKIGQGLLNMREMAEVAGVKIAIASTIG
                     KGTSITLAI"
     gene            complement(4671..6002)
                     /locus_tag="VFW59_05545"
     CDS             complement(4671..6002)
                     /locus_tag="VFW59_05545"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX5363711.1"
                     /translation="MDKLAAWIGDKLVGHLVFDARTGLFVFDYADAWQADPQSYPVSP
                     ALPFSRITEDAVHSRSVRNFFENLMPEGAALDAAATANGLAKSNLFGLIRVLGRESTG
                     AVALLPEGERPDANREPPREIAADELSRRIRERDHIPFNVWDGKVRLSIAGLQDKIAV
                     YMNEGRMYLAQGGGMASTHIVKPLPRRDGLATLVANEHFCMKLAETIGLRPAQASIIR
                     VPEPVLLIRRFDRRPSESGVVRTHVIDACQALDLPPAYKYERNFGSNPDVRHIRDGAS
                     LAKLFGLSHLAPAPALFNATILRWVLFQYLIGNSDAHGKNISFYVTRDGIIPAPPYDL
                     VSVVAYKDFDHDLAMGISDAFTYDDIMAFQWADFAQQCNINRTLLVREMRRMAAAVTK
                     ALQAPDDSSYTDEEKLVIASVRGFVLAQCEKMTQAAVLIPGISEGELSAGD"
     gene            complement(5995..6249)
                     /locus_tag="VFW59_05550"
     CDS             complement(5995..6249)
                     /locus_tag="VFW59_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5363712.1"
                     /translation="MSDEREEKVEFGDARQFGEIIRKQRKKIGLRIDDAALLCGVSVA
                     LLSGLENGSRPVGLPKALHVARQLGVSLFAEPKQTANPNG"
     gene            complement(6456..6956)
                     /locus_tag="VFW59_05555"
     CDS             complement(6456..6956)
                     /locus_tag="VFW59_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX5363713.1"
                     /translation="MMLELWRKISNRWLYLAGALFASGLMGFGLYLQYVEHQDPCPLC
                     MVQRVVFIAILALFALAALHGPKRLGQRIYAALIGLLSLSGIGVAARHIWIQNLPKDQ
                     VPACGPGLDYMLDTMPMSDVLKELMHGSGECAEKGWTFLTLGIPEWSLLCYLALGIWA
                     VLIARR"
     gene            complement(6953..7147)
                     /gene="iscX"
                     /locus_tag="VFW59_05560"
     CDS             complement(6953..7147)
                     /gene="iscX"
                     /locus_tag="VFW59_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEX5363714.1"
                     /translation="MKWIDVRDIAIALTEKHPDVDPRTVRFVDLHNWVVELDGFDDDH
                     RHGGEKVLEAIQATWIDEAE"
     gene            complement(7152..7484)
                     /gene="fdx"
                     /locus_tag="VFW59_05565"
     CDS             complement(7152..7484)
                     /gene="fdx"
                     /locus_tag="VFW59_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEX5363715.1"
                     /translation="MTQLIVLPHVELCPNGAVIEAKPGETICDTLLANGIDIDHACEK
                     SCACTTCHVVLRKGFESLTPAEELEEDMLDKAWGLEPTSRLSCQAVVGEQELIVEIPK
                     YTINMVKE"
     gene            complement(7481..9352)
                     /gene="hscA"
                     /locus_tag="VFW59_05570"
     CDS             complement(7481..9352)
                     /gene="hscA"
                     /locus_tag="VFW59_05570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEX5363716.1"
                     /translation="MALLQISEPGLSTAPHQHRLAIGIDLGTTNSLVATVRNGISVVL
                     NDDNGRAMLPSVVRYAADGGVTAGEAAQAAQSQDPVNTIVSVKRFMGRGLKDIGEISR
                     LPYRFVDTEGMLQLRTAAGVKSPVEVSAEILKVLRERAEASLGGELVGAVITVPAYFD
                     DAQRQATKDAAKLAGLNVLRLLNEPTAAAIAYGLDNAAEGVYAVYDLGGGTFDISVLR
                     LSRGVFEVLAANGDSALGGDDFDQRIHCWLLEKNNLSALSPQDTRLLLTHARAAKEQL
                     TDHVETRITAVLASGELIDNVLTRSEFQAMTQNLVQRTLQPLRRALRDAKLGVADIKG
                     VVMVGGATRMPQVQQAVGEFFGQLPLNNLDPDKVVALGAAIQANLLAGNRSGDEWLLL
                     DVIPLSLGIETMGGLSEKIIPRNSTIPTARAQEFTTFKDGQTAMSIHVVQGERELVSD
                     CRSLAKFELRGIPAMVAGAARIRVTYQVDADGLLNVAAREMSSGVESHITVKPSYGLS
                     DAEITRMLQDSAQHAQDDMLARAVREQQTEAMQLLEAVQHALVQDGVLLNEAERAVIQ
                     SKIDSLKALLQQADHQLIKRGIEALNLASTEFAQRRMDRSVNSVLAGHNISDLGGLK"
     gene            complement(9430..9963)
                     /gene="hscB"
                     /locus_tag="VFW59_05575"
     CDS             complement(9430..9963)
                     /gene="hscB"
                     /locus_tag="VFW59_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEX5363717.1"
                     /translation="MSFDFQKNHFQLFGLAPSFEIDASRLDQHYRALQAQVHPDKFAH
                     LSEAEQRLAMQQSTLVNEAYQTLRNPLARARYLLKLQGVDTQEETNTAMPLDFLMQQM
                     EWREAIAEAARASDAAALDALEAHLRRETQSLESRLAGKIDAEHNYADAAGLVRKLRF
                     MEKLAEEIHSAYDEIDN"
     gene            complement(9963..10286)
                     /gene="iscA"
                     /locus_tag="VFW59_05580"
     CDS             complement(9963..10286)
                     /gene="iscA"
                     /locus_tag="VFW59_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEX5363718.1"
                     /translation="MAITLTEKAAKHVSNFIAKRGKGVGLRIGVRTSGCSGMAYKLEF
                     ADEIAGDDIQFDSNGVKVLVDPQSLPYIDGMELDYAREGLNEGFKFNNPNVKDSCGCG
                     ESFKV"
     gene            complement(10367..10783)
                     /gene="iscU"
                     /locus_tag="VFW59_05585"
     CDS             complement(10367..10783)
                     /gene="iscU"
                     /locus_tag="VFW59_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEX5363719.1"
                     /translation="MAYSEKLLDHYENPRNVGSMDKDSDEVGTGMVGAPACGDVMKLQ
                     IKVGKDGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTVDQALAIKNTQIAEELALPPV
                     KIHCSILAEDAIKAAVADYKSKHGGNVETSACKGSM"
     gene            complement(10862..>11627)
                     /locus_tag="VFW59_05590"
     CDS             complement(10862..>11627)
                     /locus_tag="VFW59_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5363720.1"
                     /translation="MAVNNEIGVVQDIAAIGEMCRARGIVFHVDAAQATGKVEIDLQK
                     LKVDLMSFSAHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGMRSGTLATHQIVGM
                     GEAFRIAKEEMAQENERVRMLRDRLLNGLKDMEEVHINGDMEQRVPHNLNLSFNFVEG
                     ESLIMAISDIAVSSGSACTSASLEPSYVLRALGRSDELAHSSIRFSIGRFTTVEEVDY
                     VIELLKSKIGKLRELSPLWEMYKDGIDLNTVQWAAH"
BASE COUNT         2374 a         3506 c         3186 g         2561 t
ORIGIN      
        1 atgagcgttt cgatgtatca caaccccaaa tgcagcaaat gcagggcaac cttaaccctg
       61 ctgcaggaaa aagggataga gccggaactg attctctatc tggaaaccgc accttcagcg
      121 gcagcactag aggaagtgct gaaaaaactg gataaaggcc caaaagacat aatcaggttc
      181 aatgaagaag ttgcaaaaga actcggcatt tccccggcag atgagcgacc tgatcccgaa
      241 tggctgcaaa ttatttcagg caacccaaag ctgctggagc gcccgatagt gatcaatggc
      301 gacagagccg caatcggaag accgcctgag cgtgttctcg aaattctgta agtttttggg
      361 agaggatgtc ttggcttttg tgtgagttga atttactaat ctagtggcat ccattttcca
      421 agctggatag taatgcggtt cagcagcaca tcagtcggca caatgaattt gaaacgagat
      481 ttgccaatga ctcatcctcg gccattgcag acagcggttg ttgatttgta ggatgaccga
      541 attgaggtac atttcagaca ttcggattag tatgtttctg tagtttatgg ggattcacca
      601 aaaagtcagt atgtatcgac catattactt ttggcgaaca attttcgtta tcgccggcat
      661 ttgtcttgct gggtgtatga atcgcccccg attcactccg tcaatcgacg actccggcaa
      721 gaaagcctgc ctggaccact ccaatccctt aatgacttga taatattgac tatctaacag
      781 gaatgcggga aagcgactcg ttctctataa catatctcac cagatctgcc acattattca
      841 agcccagttt gcaaagtatg tgcgccttgt gcgtgctgac cgttttgtcg ctaatggcca
      901 attgattggc tatagacttg atgcactttc cctctgcgat cagcggcaaa atctgcaact
      961 ctcggtcgga aaggatcgac tccattttgg ctagtgccgt tgaattcctg gcatatacca
     1021 gttgttcagc cattttcgga tcgagatacc gcccagtagt agctacttta cgtatcgctc
     1081 caatcagtgt ctgcggagcg caatgcgtat taatgaaacc ggaggcgccg gccttcatcg
     1141 cccgcatcac agacatgaca tcgtcgtgca tgccgagaac cagcaccggc aaatcaggat
     1201 gcgcgccctt gattcgggca attaactctc cctcataaac ggccggcatg ggcatgccca
     1261 gcaagagcac atcgacgtaa aaagtctgca acttgttcaa caaccccgaa ctacaagaca
     1321 cttctgcaac cacctcaata tccggatcgg aatgaaacag ttgaacaatg cctgctctga
     1381 gcaaatcatg ttcatgcgcc aacatcaaac gaattcgcat agccatctcc actaattttg
     1441 ttgaccgagc gaggagactg acgccgaact cacccacccc tgcggcataa accgcagtat
     1501 ttgtatgata ttaaaatggg gggggagtaa atatccacaa ctcagagtag ttccactacc
     1561 gggataccgg tagtccgagc aagatactga agactagttg atggcgaggc cgttgttgat
     1621 cgcgtagcgg gtaagctcgg cgacattatg caaattgagt ttgcgcatga tgttgcgccg
     1681 atgcacttca acggtggatg gggcgatgtt cagctgatcc gcaatcatcg ccgaggtcag
     1741 accttcggcc acgagttgca gcacctgacg ctcccgattg ccgagattgg aggagagtgt
     1801 tgcgtgcggg gacatagccc cagtgaccgc ctccacgaca tcgggacaaa ggtatttgcg
     1861 attgtgcttt agcgcatcga tcgcccgcag caattcgtcg gtggcttctg ccttggtgac
     1921 ataggcagaa gcgccggcat tcatcatgtc catcacatat cgccggtcgg agtatgacga
     1981 aagtgcgatc actttgacgt tcggacaagc cgcgagaaga tttcgcgtaa tttcaatgcc
     2041 gttcatcttg gggatgccga tatccatgca cacgattttg gccgaactct tgcgcgcaag
     2101 atcgatcacc tcgaatgggt tgccgcattc cccgacgact tccagattcg gcattttctc
     2161 cagcaaactc cgcagcgcat cgcggaatat tttgtggtca tcgacaagca ttatgcgaac
     2221 gttttccatg tacacccacc aataattaag tcgttttcca agccgcacta ctagattgct
     2281 aacgtaatac tggttccttt gcctatggtc gatgcgatgg cgattttgac gccggcaact
     2341 tcggccattt cacgcatatt caatagccct tggccgattt ttctggtttt gcccagttgt
     2401 gcaggatcga aacccactcc gtcgtcggtg atggtcagga tgttagggcg aatttcattg
     2461 caaagcgata tccggatcga ttcggcgctg gagtgtttgg agcaattcgt cagcgcctcc
     2521 tgaagaatcc tgaacagcaa tgactcgatt tgtggcgtgt atcttgtgtc tctcttctca
     2581 cactcaaaat agatttggat gcctgttcgc cgagcgaaca aaaaagcgta gttctccatc
     2641 gccgctatca gcccggcata atcaagcagt ggcgggcgca tttcggcacc gatctcacgg
     2701 atgctggcgg acgtgtcaga aatcaacgcg cgaatatcct ccagacgctc ggtcaagtct
     2761 gtcgaggtcc cttgagcaat ttctgcggca atgacgttca gattgatatc gatagcggca
     2821 aggttcggac tggttcggtc atgtaattcg caagcgaggc gctgccgtgc atcttcctgt
     2881 gcggcgacaa ggttgcgcga tatttcctcc aggcgcatca agtggttgat acgctcccgc
     2941 tccatctttt ttcgctcggt gatgtcgcgt attgccgcgc aaacatgccc cactttttca
     3001 tccgtatctg gaaggcgcga cagactcaca tcgaccggga actccgtccc atccttgcgg
     3061 cagccgcgca agccccttgc actgaccgac ttcaagctat tcgtagtgga aacggcaaaa
     3121 tccgtacgtt tgtctacatg gttttcgcgc acgtcaggtg gcagcagccg ttcgaccggc
     3181 agtccgacca gttcgccctt cgcgtaaccg aacatggcct cgatccgagc attaacctgc
     3241 gtgatcacgc cgcgcgcgtc tgtcaccacc atgccgtctg gtgccgaatg aagtatgctg
     3301 cggtaccatg tctcggtctg tttgagatgc tcttgcgatg ccgccaactc ttccgtttgc
     3361 accctcaggt tgcgcgcact ttcccctagg ttgctgttaa gttcctcgat ctgacacaac
     3421 cgctccttca gcagagcctc ggcctgcttg cgctcggtga tgtcgagcat gatgccgtgc
     3481 cagaggatgc tgccgtcggc ctcgcgctcc ggcaaagcac gcgcatcgag ccaacgttct
     3541 ggctgccccg ggcgacagac gcgggattcg atgcggaacg gcgtcatcgt gcgcgcagat
     3601 tctgctgtgg cggcttcgat gtgcatccga tcatcgggat gccagaagct atggagcgga
     3661 gctatgtcgt cctgcacgtc ttccggcttg aggccgtaga atttttcgat ggaggggctg
     3721 atgaagggaa agctggcatg cccgtcgggc gacagacgga aggtgtaggc aaaaccgggc
     3781 atgttgtcga taaagtgctt cagccgccgt tcggcttcgc ggatgacggt gatttcccgt
     3841 ccgaaagcga tggtgccgac gatctgtccg cctacatcgc gctccggcac gacgaaaatc
     3901 tggtggtaat ggaaagcgcc gttctcggtc ggcacgacaa gttcgatgtc cacctggctt
     3961 ccgctatccg ctgcgcgcgc agcggctcgc tcgagctccg caaaacgacc gtccgaccac
     4021 acctcgccag gacgcttgcc gatcacctct ttcgcgctgg cgagttccaa ctgtttcagc
     4081 aatctatcgt tgaggtagag gatgcgtcca gcgcagtcgt agcggatgat ggaatccggc
     4141 gaactttccg ctaggctgcg gaattcctgc tcccgtacgg cgatagccag ttccaactgc
     4201 ttgcgcccgg tgatgtcgcg accgatggcg agagctccaa tcacttcgcc gttctcaccg
     4261 cgctcgggaa ccttagcaat ttgtcgcagg ctctcgtcta ttcgcatagt ctgcccttca
     4321 tttttaataa aaacacttaa agccaattgg ataataaaat cgtgtcacac atagacgata
     4381 gttaaaaact aataaacaat gaacatttga tgcaccaaaa ttaatttata acgccccaaa
     4441 atgtatacga catttcgcaa ttaatacata tgtcgaaatg cctatacatt tattcctatg
     4501 tatttcaaat tccaaatctc ttaccactcg agctccttgc atctactgtg acaagccaag
     4561 cctgagagtg tgcggaatct tgtgtattac ccaactgtcc acttcacggt aaatagcgga
     4621 agcttatgag gtttccggta gagtctgctt tggcctgcgg attcaaccct tcaatccccc
     4681 gcactaagct ccccctccga tatccccgga atcagcactg ccgcctgcgt catcttctcg
     4741 cactgcgcaa gcacaaaacc acgcacgcta gcgatcacaa gcttttcttc atcggtgtag
     4801 ctcgaatcgt caggagcttg cagcgccttc gttactgcag cagccatgcg gcgcatttcg
     4861 cgcaccaaca gcgtccggtt gatgttgcac tgctgagcga agtcggccca ttgaaaagcc
     4921 atgatgtcgt cgtacgtgaa ggcatcggag atgcccatgg cgaggtcgtg atcgaagtcc
     4981 ttgtacgcga cgaccgacac gaggtcgtag ggcggcgcgg ggatgattcc gtcgcgcgtt
     5041 acatagaacg agatgttttt gccgtgcgcg tcgctgttgc cgatcaggta ctgaaacagc
     5101 acccagcgca ggatggtcgc gttgaacagc gccggggccg gcgccagatg gctcagcccg
     5161 aatagtttgg ccaagctcgc gccgtccctg atatgacgga catccgggtt gctgccaaag
     5221 ttgcgctcgt atttgtatgc cggcggcaag tccagcgcct ggcaggcatc gatgacatga
     5281 gtgcggacga cgccggattc cgacggccgc cggtcgaaac ggcggatcag caagaccggc
     5341 tccggcacgc ggataatgct cgcctgcgcc gggcgcaggc cgatggtttc cgcgagcttc
     5401 atgcagaaat gctcgttggc caccagtgtg gccaatccgt cacggcgcgg gagcggtttg
     5461 acgatgtgcg tcgatgccat tccgccgccc tgggcgagat acatcctgcc ctcgttcata
     5521 tacacggcga tcttgtcctg cagtcccgca atcgacaggc gcaccttgcc gtcccatacg
     5581 ttgaacggaa tgtggtcgcg ttcgcgtatg cggcgggaca attcgtcggc ggcgatttcg
     5641 cggggcggct ctcgattcgc atcgggccgc tcgccctcgg gcaacagtgc gacggcgccc
     5701 gtgctttccc gtcccagtac acggatcagg ccgaacaggt tgctctttgc caggccgttg
     5761 gcggtcgcag cggcatccag cgccgcgcct tccggcatca ggttttcgaa gaaatttcgc
     5821 accgatctcg aatgaacggc atcctcggtt atgcgcgaga acggcaaggc cggagaaacc
     5881 ggataggact gcgggtcggc ctgccaggcg tcggcataat cgaagacaaa caatcccgtc
     5941 cgcgcatcga aaacgagatg cccgacgagc ttgtcgccta tccatgcggc cagcttatcc
     6001 attggggttc gccgtctgtt tcggctcggc aaacagggag acgccaagct ggcgcgcgac
     6061 atgcaaggcc ttgggcaggc caacaggtcg cgatccattc tccagcccgg aaagcagcgc
     6121 cactgacaca ccgcacaaca gcgcggcatc gtctatcctc agcccgattt ttttccgctg
     6181 cttgcggatg atttcgccaa actgccgggc gtctccgaat tcgaccttct cttctcgttc
     6241 atcagacata ggcatattca tccatatatt tctctcatac gagaaatata tccatacacc
     6301 agtagcgagt caacgcaatt tctcgcaaac gagaaattaa ttacttaagc agcgatgagc
     6361 aatacaaatt cctcgtgcgc gagaaatatc gatgaagaat agcgtgggac tttaagggcc
     6421 gctctataac tcgtcacacc ggcgaaggcc ggtgttcagc ggcgtgcgat cagcaccgcc
     6481 cagatgccca gcgcgagata gcacagcagc gaccactcgg ggatgccgag ggtgaggaag
     6541 gtccagccct tttccgcgca ttcgccggag ccgtgcatca gctctttcag cacgtcggac
     6601 atcggcatgg tgtccagcat gtagtccaga ccggggccgc aggcgggcac ctgatccttg
     6661 ggcaggttct ggatccagat atggcgggcc gccacaccga tgccggacag cgacagcagg
     6721 ccgatcagcg ccgcatagat acgctgcccg aggcgcttcg gcccgtgcaa tgcggcgagc
     6781 gcgaacagcg ccaggatcgc gatgaacacc acgcgctgca ccatgcacag cgggcaggga
     6841 tcctgatgtt cgacgtattg cagatacagg ccgaagccca tcaggccgct ggcgaacagc
     6901 gcacccgcga ggtacagcca gcgattggag attttgcgcc agagctcaag catcattccg
     6961 cctcgtctat ccaggtggcc tgaatcgctt ccagcacctt ctcgccgcca tggcggtgat
     7021 cgtcgtcgaa accatccagc tcgaccaccc agttatgcag atcgacgaag cgcaccgtgc
     7081 gcggatcgac gtcaggatgt ttttcggtca gcgcgatcgc gatgtcgcgc acatctatcc
     7141 atttcatctt ttcactcctt gaccatgttg atggtgtact tgggtatctc gacgatcagt
     7201 tcctgctcgc ccaccaccgc ctgacaggac aggcgcgagg tcggttccag cccccaggct
     7261 ttgtccagca tgtcctcttc gagttcctct gcgggcgtca gactttcgaa gcccttgcgc
     7321 agcacgacat gacaggtggt gcaggcacag gacttctcgc aggcgtgatc gatgtcgatg
     7381 ccgttggcaa gcagggtgtc gcagatagtc tcaccgggct tggcctcgat gacggccccg
     7441 ttcgggcaga gctctacgtg cggcaatacg atcagttgcg tcatttcagc ccccccagat
     7501 ccgatatgtt atgcccggcc agcacgctgt tcacgctccg gtccatgcgg cgctgggcga
     7561 actcggtgct cgccagattc aatgcctcga tgccgcgttt gatgagctgg tgatcggcct
     7621 gttgcagcaa cgccttcagg ctgtcgatct tgctctggat gacggcgcgc tcggcttcgt
     7681 tcagcaacac cccatcctgc accagggcat gctgaaccgc ttcgagcaac tgcatcgcct
     7741 cggtctgctg ctcgcgcacc gcgcgcgcca gcatatcgtc ctgcgcgtgc tgcgccgaat
     7801 cctgcagcat gcgggtgatt tcggcgtcgg aaagaccata ggatggcttg accgtgatat
     7861 gcgactccac gccgctgctc atctcgcgcg ccgccacgtt cagcagcccg tcggcatcca
     7921 cctgataggt cacacggatg cgcgcagccc cggccaccat cgccgggatg ccgcgcaact
     7981 cgaactttgc cagcgaacgg caatcgctga ccagctcgcg ctccccctgc accacgtgga
     8041 tgctcatcgc ggtctgcccg tccttgaagg tggtgaattc ctgagcgcgc gcggtgggga
     8101 tggtgctgtt gcgcgggatg atcttctcgg acagcccgcc catggtctcg atccccagac
     8161 tcagcgggat cacgtcgagc agcagccact cgtcgccgct gcggttgccc gccagcaggt
     8221 tggcctggat cgccgcaccg agggcgacga ccttgtccgg gtcgaggttg ttcagcggga
     8281 gctggccgaa gaattcaccg accgcctgct gcacctgcgg catgcgcgtc gcgccgccga
     8341 ccatcaccac ccccttgata tcggccaccc ccagcttcgc atcgcgcaac gcacggcgca
     8401 acggttgcag ggtgcgctgc accagattct gcgtcatggc ctgaaactcg ctgcgcgtca
     8461 gcacattgtc gatcagctcg ccgctggcca gcaccgcagt gatgcgcgtc tcgacatggt
     8521 cggttagctg ctctttcgcg gcgcgcgcat gggtcagcaa caagcgggta tcctgcgggc
     8581 tgagcgcgga gagattgttc ttttccagca accagcaatg gatgcgctgg tcgaagtcgt
     8641 cgccgcccag tgcggaatcg ccgttggccg ccagcacctc gaacacgccg cgcgacagac
     8701 gcaggacgga gatatcgaag gtgcctccgc ccagatcgta gaccgcatac acgccctcgg
     8761 cagcgttatc cagaccatag gcgatggctg ccgccgtcgg ctcgttgagc aggcgcagca
     8821 cgttgagccc ggcgagcttc gccgcatcct tggtagcctg ccgctgcgca tcgtcgaaat
     8881 aggccggcac ggtgatcacc gcgcccacca gttcgccgcc cagcgaggcc tcggcccgtt
     8941 cgcgcaacac cttgagtatc tcggcagaaa cctccaccgg actcttcacg ccggctgccg
     9001 tgcgcagttg cagcatgccc tctgtatcca cgaaacggta cggcaagcgg ctgatctcgc
     9061 cgatgtcctt caggccgcgc cccatgaaac gcttcaccga cacgatggtg ttgaccggat
     9121 cctgactctg cgccgcttgc gccgcctcgc ccgccgtcac gccgccatcc gccgcgtagc
     9181 gcaccaccga aggcagcatc gcacgcccgt tgtcgtcgtt gagcaccacg ctgatgccgt
     9241 tgcgcaccgt tgccaccagc gagttggtgg taccgagatc gatgccgata gccagccgat
     9301 gctggtgcgg tgcggtggag agaccgggtt cggatatttg caggagagcc atttaaaatt
     9361 ggtcagtcgt aagtggtcag tggcgaacgg ggggactact cacgacgtcc cactcaccac
     9421 ttactggttt taattatcta tctcgtcgta ggcggaatgt atctcttccg cgagtttttc
     9481 catgaagcgc agcttgcgca ccagtccggc ggcatccgca taattatgtt cggcgtcgat
     9541 tttacccgcg agccgacttt cgagtgactg ggtttcgcga cgcaggtgcg cctccagcgc
     9601 gtccagcgcc gccgcgtcgc tcgcccgggc agcctcggcg atcgcctcgc gccactccat
     9661 ctgctgcatc agaaaatcca gcggcatcgc cgtgttggtc tcttcctggg tgtccacacc
     9721 ctgcagcttc agcagatagc gcgcgcgcgc cagcggattg cgcagcgtct gataggcctc
     9781 gttcaccagc gtggactgtt gcatcgcgag gcgctgttcg gcctcggaca gatgggcgaa
     9841 tttatcggga tgaacctgcg cctgcaaggc gcgatagtgt tgatccagcc gactcgcgtc
     9901 gatctcgaag gacggtgcga gaccgaacag ctggaagtgg tttttctgga agtcgaacga
     9961 catcaaacct tgaagctttc gccgcagccg caggaatcct tgacgttcgg attgttgaac
    10021 ttgaagccct cgttcaatcc ctcgcgcgcg tagtccagct ccatgccgtc gatgtaaggc
    10081 aggctttgcg ggtcgaccag caccttcacg ccgttgctgt cgaactggat atcgtcgccc
    10141 gcgatctcgt cggcgaactc cagcttgtaa gccatgccgg aacagccgct ggtgcgcaca
    10201 ccgatgcgca aacccacccc cttgccgcgt ttggcaatga aattcgacac atgtttggcc
    10261 gctttttcgg tcagcgtgat cgccatgatt ttctctccgt tttcaccctc tcccctctcc
    10321 cgcacgcggg cgagggggga cagagtctcg ctacgcgagg tttacgttac atgctgccct
    10381 tgcaggccga agtttccacg ttcccgccat gcttggactt gtagtccgcc acggcggcct
    10441 tgatcgcgtc ttccgcgagg atggaacagt ggatcttcac cggcggcaat gccagctcct
    10501 cggcgatctg cgtgttcttg atcgccagcg cctgatccac ggtcttgccc ttgacccatt
    10561 cggtcaccag agaactggag gcgatcgcgg agccgcaacc gtaggtcttg aacttcgcat
    10621 cctcgatcac gccatccttg ccgaccttga tctgcaactt catcacgtcg ccgcaagccg
    10681 gcgcgccgac catgccggta cccacctcat cggaatcctt gtccatcgag ccgacgttac
    10741 ggggattttc gtagtgatcc agcaattttt cgctataagc cattttaatt ctcctaggat
    10801 tgaggagtga ggattgagat aaaccgcgct ccggttctat cctcaatcct cgatcctgcc
    10861 ttcaatgtgc ggcccactgc acagtattca ggtctatgcc gtctttgtac atctcccata
    10921 agggggatag ctcgcgcaat tttccgattt tgctcttcag caattcgatg acatagtcca
    10981 cttcctcgac ggtcgtgaaa cgaccgatac tgaaacggat cgagctatgt gccagttcgt
    11041 cgctgcgccc cagcgcacgc aacacgtagg acggctccag gctcgccgag gtgcaggccg
    11101 aaccgctgga aaccgcgata tcgctgatcg ccatgatcag cgattcgcct tcgacgaagt
    11161 tgaaactgag gttcaggttg tgcggcacac gctgttccat gtcgccgttg atatgcacct
    11221 cttccatgtc cttcaggccg ttcagcagac ggtcgcgcag catgcgcacg cgctcgttct
    11281 cctgcgccat ttcttctttc gcgatgcgga acgcctcgcc catgccgacg atctgatgcg
    11341 tcgccagcgt acccgagcgc atgccgcgct cgtgaccgcc gccgtgcatc tgtgcttcca
    11401 gacgcacgcg cggcttgcgg cgcacataca gcgcaccgat gcccttgggt ccgtaagtct
    11461 tgtgggcgga gaacgacatc agatccacct tgagcttttg caggtcgatc tccaccttgc
    11521 cggtcgcctg cgccgcatcg acgtggaaga cgatgccgcg cgcgcggcac atctcgccga
    11581 tcgcggcgat gtcctggacg acgccgatct cgttgttcac cgccatc
//
LOCUS       DASUIV010000149         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_871893, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000149 DASUIV010000000
VERSION     DASUIV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871893"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VFW59_05595"
     CDS             complement(<1..166)
                     /locus_tag="VFW59_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5363721.1"
                     /translation="MSVTNLLENWSQFRHFHPVDDKPHDPYKWAMAIDLDACTGCNAC
                     SVACYAENNLP"
     gene            complement(170..2380)
                     /locus_tag="VFW59_05600"
     CDS             complement(170..2380)
                     /locus_tag="VFW59_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5363722.1"
                     /translation="MIDSGFDRRDFLKVLGWGGAAVALSGCGNTSIENGKETVVSYVE
                     ASDYMIPGIGVYFNSTCAQCDAGCNINGRVREGRVLKLEGNPDSAINRGKTCGLGQAG
                     VQHHYNPDRVREPLLRNGDKGEVISWEKAYALIAEKLNGVKGEEIAFLSGGVSGHLKV
                     LLGNYLDSLGTKNHFVYEAVAPAVARAANKKAYGVEMPRFSINKARVVLSFGADFLGS
                     WVSPVHFSQQYAQFRKGVNGQRGVLVQVESKMTLTGANADRWLAVRPGTEGILALGII
                     NALGASGMSLSGDVAAAVKAYDKERVSKDTGVSAEQIDKLAALLKERTPSLVLAGSAA
                     EGYAHGSQNAAAINLLNQVLGNVGKTVEAAATVPFPQMAPAKGNTAALKALNDGLAAG
                     KYKVLFSYAANPVFTASGALKFKDSMAKAGFKVAFAHYLDETALQADLVLPLDSALED
                     WGTQVPEYMAEGAQISIQQPLMEKLHANTRGAGDILLALVKQRNPEGYKNYEDYYAYL
                     RSALLQNKAALGGAGADDDTFWNETLTKGIVKLSGSAGALSGKASAAGLVLPAPAAAD
                     AQYPLQLIPGVTASLRDGRYANQPWLQESPDPLTTIVWDSWVEIHPKKAAELGIVEGD
                     LVEVTSKTGSIKAQAYLFPGIHPDAISVPLGRGHEAMGRYAKGYGVNPFQILDAVFDQ
                     ETGELAMHETRVKVSKVGQRVIVVKDEGAAGGKQMGKKIAVRVSTDEVDLSEEV"
     gene            complement(2423..3088)
                     /locus_tag="VFW59_05605"
     CDS             complement(2423..3088)
                     /locus_tag="VFW59_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5363723.1"
                     /translation="MSSYAADAPQPTLTTADELGPKVEQPIEFPHDRHAGLRNPNDPT
                     NSDPLKGGMEIDCQYCHTYARRSAVAGIPPLQKCIGCHQSIETVRNKPRIVELFKYWD
                     AKKPVPWKKVHDLPDFVRFNHERHIKRFVEQQNRPTQEACGYCHGDVKTMTVARRSKP
                     LTMGWCATCHEKAHAVTAAGGTSVGNQAYWYSFNKPKEQKADVVPSEVKAQGPNDCWQ
                     CHK"
     gene            3445..4074
                     /locus_tag="VFW59_05610"
     CDS             3445..4074
                     /locus_tag="VFW59_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363724.1"
                     /translation="MNEEQAVLNFFAQPENLPLGLSVAEQMDGIREQLNCRYWRELLP
                     RLDALINALALPWNIALTEDKNAPDNLVGLHCGLRTEQPIYLRPMLEQQYLGGTWRIY
                     FGLMWSATPTPEQLALPAVGALKQTLQDAGYKNNANFLAWQWTGFHPRRRDFLLRYAQ
                     HPETVLEETVAILNPLLTEHRNSIEQANDALQAAPRSMAVSLDQLRSKR"
     gene            complement(4071..4817)
                     /locus_tag="VFW59_05615"
     CDS             complement(4071..4817)
                     /locus_tag="VFW59_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX5363725.1"
                     /translation="MLLDELRDYNASLLQTALFKDYCPNGVQVEGRAEVRRIATGVTA
                     SQQLLEEAIAWGADAILVHHGYFWRSEEAVITGIKKRRIAQLLRNDVSLLAYHLPLDA
                     HAELGNNVQLGRLLGLVEQGRFGEQNIACLGSLAQPQTLMQFTQQIAHGLQRSPQVIG
                     DGARPIKKVAWCSGGAQGYFEAAIAQGVDAYITGEISEQCFHLANETGVAFIAAGHHA
                     TERLGIQALGLHLARQFGLEQRFFDQANPI"
     gene            4858..>5397
                     /locus_tag="VFW59_05620"
     CDS             4858..>5397
                     /locus_tag="VFW59_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5363726.1"
                     /translation="MRKHWLLFTQTVTIALALLFVVHTLKPELLPNAARSGVVTLYEN
                     APQSDEKTSPTATLSLAAHKAMPAVVNIFTSTIVKTPANPFLDDPRFKFFFGDQLDNE
                     PQSNSSLGSGVIVSPDGYILTNHHVVEAADQIEVALEDGRTAKAHVIGSDPETDLAVI
                     KIDLPDKLPAITFGHPEHAQ"
BASE COUNT         1122 a         1752 c         1429 g         1094 t
ORIGIN      
        1 ccggcaggtt gttctccgca tagcaagcca cgctgcaggc gttgcaaccg gtacaggcat
       61 ccagatcgat tgccatcgcc cacttgtacg ggtcatgcgg cttgtcatcg accgggtgaa
      121 aatgacggaa ttgtgaccaa ttctctaata gattagttac ggacatacct tagacctcct
      181 ccgaaagatc gacttcatcg gtggacacgc gcacggcaat cttcttgccc atttgcttgc
      241 cacctgcggc accttcatcc ttgaccacga tgacgcgctg gccgaccttg ctgaccttca
      301 cgcgcgtttc atgcatcgcc aattcaccgg tttcctgatc gaacaccgcg tccagaatct
      361 ggaacgggtt cacgccatag cccttggcat aacgccccat cgcctcgtga ccgcggccca
      421 gcggcacgga gatcgcgtcc gggtggatgc cggggaacag ataagcctga gccttgatcg
      481 agccggtttt ggatgtaact tcgacgagat cgccttcgac gatacccagc tcggccgcct
      541 tcttcggatg gatctcgacc caggaatccc acacgatggt ggtcaatgga tccggcgact
      601 cctgcagcca gggctggttc gcatagcgac cgtcgcgcag gctggcagtc acacccggga
      661 tcagctgcag cggatattgc gcatcggcag ccgcaggcgc cggcagcacc agaccggcgg
      721 cactcgcctt gccggacaag gcacccgcag aaccggacag tttcacgatg cccttggtca
      781 gcgtctcgtt ccagaatgta tcgtcatccg cacccgctcc gcccagcgcc gccttgtttt
      841 gcaacagcgc actgcgcaga taggcgtagt agtcttcgta gttcttgtag ccttcaggat
      901 tgcgctgctt gaccaaggcc agcaggatgt cgcccgcacc gcgcgtattg gcatgcagct
      961 tttccatcag cggctgctga atgctgatct gcgcgccttc ggccatgtac tcgggaacct
     1021 gcgtacccca gtcttccagc gcggaatcca gcggcaacac cagatcggcc tgcaacgcgg
     1081 tctcgtccag atagtgagcg aacgccacct tgaagccggc cttggccatg ctgtccttga
     1141 acttcaacgc acccgatgcg gtaaacaccg gattggccgc ataactgaac agcaccttgt
     1201 acttgccagc cgccaagccg tcgttaagcg ctttcaacgc ggcggtattg cccttggccg
     1261 gagccatctg cgggaacggc acggtcgctg cggcctcgac ggtcttgccg acgttgccca
     1321 gcacctgatt gagcaggttg atcgcggcgg cattctgcga gccgtgcgca taaccttctg
     1381 ccgcgctgcc tgccagcacc agactaggcg tacgctcctt gagcagggcc gcgagcttgt
     1441 cgatctgttc tgccgacacg ccggtgtcct tgctgacacg ctccttgtcg taggccttca
     1501 ccgccgccgc cacgtcaccg gacagactca tgcccgaagc gcccagcgcg ttgatgatgc
     1561 ccagcgccag aataccttcg gtacccggac gcactgccag ccagcgatcc gcattcgcac
     1621 cggtcagcgt catcttggac tcgacctgca ccagcacgcc gcgctgcccg ttcacgccct
     1681 tgcggaactg cgcatactgc tgcgagaaat gcaccggaga aacccagctg ccgaggaagt
     1741 cggcaccgaa ggacagcacc acacgggcct tgttgatgct gaaacgcggc atctccacgc
     1801 cgtaggcttt cttgttggcc gcacgcgcca ctgcaggcgc gaccgcctcg tagacgaaat
     1861 gattcttggt gcccaggctg tccagataat tgcccagcaa caccttgagg tgaccgctca
     1921 cgccgccgct caggaaggcg atttcttcac ccttcacgcc gttcagcttc tcggcgatca
     1981 gcgcataggc cttttcccag gagatcactt cacccttgtc gccgttgcgc aacaacggct
     2041 cgcgtacgcg atccggattg taatgatgct gcacgcccgc ctgccccaaa ccgcaagtct
     2101 tgccacggtt gatggcggaa tcgggattgc cttccagctt cagcacgcgg ccttcgcgca
     2161 cccgcccgtt gatgttgcaa cccgcatcgc actgcgcaca ggtcgaattg aaatacacgc
     2221 cgatgcccgg gatcatgtag tccgaagctt ccacgtagga aactacggtt tccttaccgt
     2281 tctcgatgga ggtgttgccg cagccgctca gcgctaccgc tgcgccgccc caacccaaca
     2341 ccttcagaaa atcacgccga tcaaaaccac tgtcaatcat cacaacctct ctggaaaatt
     2401 cttgaaatgt catgcccgca cattacttgt ggcattgcca gcagtcgtta ggcccctgcg
     2461 ccttgacttc gctaggaaca acatcggcct tctgctcttt cggcttattg aagctgtacc
     2521 aataggcctg attgccgacc gatgttccgc ctgccgccgt cacggcatga gccttctcat
     2581 ggcaggttgc gcaccacccc atggtcaaag gtttgctgcg acgtgcaacc gtcatggtct
     2641 tcacgtcgcc gtggcaatag ccgcaggctt cctgagtcgg acggttctgc tgttcgacga
     2701 aacgcttgat gtggcgctcg tggttgaagc gcacgaagtc gggcaggtca tgcaccttct
     2761 tccagggaac cggcttcttg gcatcccagt acttgaacag ttcaacgatg cgcggcttgt
     2821 tgcgcaccgt ctctatgctc tggtgacagc cgatacattt ttgcaacgga ggaataccgg
     2881 caaccgcact acgtctggcg taggtatggc aatactggca atcgatctcc atgccgccct
     2941 tgagcggatc gctattggtc gggtcattcg gattacgaag acccgcatgg cgatcatggg
     3001 gaaactcgat gggttgttcg acttttggac ccaactcgtc cgcagtcgtt agggtaggtt
     3061 gaggagcatc agctgcgtaa ctgctcatgg cagcaaacaa tccagtcaac agaatagaca
     3121 aacgtaagaa acggtgcatc gtaagggctc ccccagcaaa caaatgatcc catgccggct
     3181 tgatgccagc atgattgaac atgtaaatcg gattattttt tggtgcggtg tgtcgcaata
     3241 aaattattat tgtttaagct acacttcccg cccgaatttt agcggcggcg cgaccacacg
     3301 acaggataat gtccactctg aaaaatattg tcaacaccgt aaattacacc tcagctcagc
     3361 agggtaaaaa aaagctttac gcgccacata attgcagcaa tcgctaatat acatttgaga
     3421 cattgatcca cgggaatttt caccatgaat gaagaacaag ccgtcctgaa ttttttcgcc
     3481 cagccggaga atttaccgct ggggctgtcc gtcgccgagc agatggacgg gatacgcgag
     3541 cagcttaatt gccgttactg gcgcgagctt ctcccccgcc tcgatgcgct gatcaacgct
     3601 ctcgcattgc cctggaacat cgcgctcacc gaagacaaga atgcgcccga caatctggtc
     3661 ggcctgcatt gcggcttgcg tacagaacag ccgatctacc tgcgcccgat gctggagcag
     3721 caatatctgg gcggcacatg gcgcatctat ttcggcctga tgtggagcgc cacgcccacg
     3781 ccggagcagc tcgccttgcc cgccgtaggc gcgctcaaac aaacactgca agacgcgggc
     3841 tacaagaaca acgccaactt cctcgcctgg cagtggaccg gctttcatcc gcgccgcaga
     3901 gattttttac tgcgctacgc gcagcatccg gaaaccgtac tcgaagagac cgtggcgatt
     3961 ttgaacccgc tgctgaccga gcaccgcaac tcgatcgaac aggccaacga cgccctgcag
     4021 gccgcaccgc gcagcatggc agtttcactg gatcagttgc gcagcaaacg ctagatcgga
     4081 ttggcctgat cgaagaagcg ctgctccagt ccgaattgcc gggccagatg cagccccagc
     4141 gcctgtatgc ccagacgctc ggtcgcatgg tgtccggcgg cgatgaacgc cactccggtt
     4201 tcgttcgcca gatggaaaca ctgctcggaa atctcgccgg tgatgtaagc atccactccc
     4261 tgcgcgatgg ccgcctcgaa atagccctga gcgccgccac tgcaccacgc gactttcttt
     4321 atcggccttg cgccgtcacc gatcacctgc gggctacgct gcaaaccatg cgcgatctgc
     4381 tgggtgaact gcatcagcgt ctgaggctgc gccaacgaac ccaggcaggc gatgttctgc
     4441 tcaccgaatc tcccctgctc gaccagcccc aacaacctgc ccagctgtac gttgttgccc
     4501 agctcggcat gggcatccag cggcaggtga taggccagca ggctgacatc gttgcgcaac
     4561 agctgcgcga tgcggcgctt cttgatgccg gtgatcacgg cctcttcgct gcgccagaaa
     4621 taaccgtgat gcaccagaat cgcatccgcc ccccaggcga tggcctcctc cagcagttgc
     4681 tgcgaggcgg tcactcccgt ggcgatgcgc cgcacttcgg cacgcccctc cacctgcacg
     4741 ccgttcgggc aataatcctt gaacaaggcg gtttgcaaca ggcttgcgtt ataatcgcgc
     4801 agttcatcga gcagcatgag tttcccttaa tccagattcg aacgaattca tcataacatg
     4861 cgtaaacact ggctcctgtt cacccagacc gtcaccatcg cgctggcgct gttgttcgtc
     4921 gtccacaccc tgaagcctga attgctgccg aacgcggcgc gcagcggcgt ggtcacgctc
     4981 tacgaaaacg cgcctcaatc cgatgaaaaa acatcaccga cggcaaccct gagcctggcc
     5041 gcacacaagg ccatgcccgc cgtggtcaac attttcacca gcaccatcgt caagacgccg
     5101 gccaatccgt ttctggacga cccgcgcttc aagttcttct tcggcgatca actcgacaac
     5161 gagccgcaaa gcaattccag cctcggctcg ggcgtgatcg tcagtccgga cggttacatc
     5221 ctgaccaatc accatgtcgt ggaagccgcc gaccagatcg aagtcgcact tgaagacgga
     5281 cgcaccgcca aggcgcatgt catcggctcc gatccggaga ccgatctggc cgtgatcaag
     5341 atcgacctgc ccgacaagct ccccgcgatc acctttggcc atccagagca cgctcag
//
LOCUS       DASUIV010000150         8456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1152787, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000150 DASUIV010000000
VERSION     DASUIV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152787"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1047
                     /gene="dusB"
                     /locus_tag="VFW59_05625"
     CDS             40..1047
                     /gene="dusB"
                     /locus_tag="VFW59_05625"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX5363727.1"
                     /translation="MQIGPYKLKNNLIVAPMAGVTDRPFRILCTRMGAGMAVSEMVAS
                     NSLLYGSEKTKRRANHEGEVDPISVQIVGADPKMLAQAARHNVDNGAQIIDINMGCPA
                     KKICNVMAGSALLQNELLVAEILEAVVAAVDVPVTLKIRTGWDRNNRNAVNIARIAES
                     CGIQALAIHGRTRACAYTGDAEYDTIRAVKAEVNIPIIANGDITTPEKAKYVLEHTGV
                     DAVMIGRAAQGRPWLFREIEYFLQTGTHLPSPEVGEIHRILVAHVQELYDFYGEHTGL
                     RVARKHISWYTKGLVGSAHFRHHMNRLETPSKQMAMVDEFFIEQAQSGTRLRYLEEMA
                     A"
     gene            1044..1295
                     /locus_tag="VFW59_05630"
     CDS             1044..1295
                     /locus_tag="VFW59_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5363728.1"
                     /translation="MTDCAVDENDMARYVRKALKDYFKDLDGEDPCCNMYDMVMDCVE
                     KPLIEMVLGHVGGNQTQAAAMLGINRNTLRKKMLQHGIA"
     gene            1389..2954
                     /gene="purH"
                     /locus_tag="VFW59_05635"
     CDS             1389..2954
                     /gene="purH"
                     /locus_tag="VFW59_05635"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5363729.1"
                     /translation="MAAIKQALISVSDKSGVLEFAQGLHSLGVKLLSTGGTAKLLSDN
                     GVPCTEVADYTGFPEMLDGRVKTLQPKVHAGILARRDLPEHVATLAKHGIPTIDLVVV
                     NLYPFAATVAKPGCTLEDAIENIDIGGPTMVRAAAKNHAHVAIVTDPSDYTSVLAEMQ
                     TNAGAVSDATRFDLMKKAFSHTAAYDSMISNYLTAIDSDGAKSEFPAQINFNFAKVQD
                     MRYGENPHQSAAFYRDLNPVAGGIADYTQLQGKELSYNNIGDADAAWELVKTFDQPAC
                     VIVKHANPCGVAIADTALNAYKLAYATDTTSAFGGIIAFNRELDEATAAQITANQFVE
                     LIIAPSASEAALKVTAAKQNVRVLTVPLSNAHNQYDVKRVSGGLLVQSPDALNVQLSQ
                     LKVVSKVQPTADQLTDLLFAWRVAKYVKSNAIVFCKGGQTLGVGAGQMSRVDSTRIAS
                     IKAQNAGLSLVNSVVSSDAFFPFRDGIDVLAQAGAKAVIQPGGSMRDEEVIAAADEHG
                     LAMVFTGYRHFRH"
     gene            3077..4351
                     /gene="purD"
                     /locus_tag="VFW59_05640"
     CDS             3077..4351
                     /gene="purD"
                     /locus_tag="VFW59_05640"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5363730.1"
                     /translation="MKILVIGSGGREHALAWRLAQGAKVQKVYVAPGNAGTALEEGLE
                     NVAITAIPDLIEFVKRENVELTVVGPEAPLAAGVVDAFRAAGLKIFGPTKAAAQLESS
                     KDFAKRFMTRHNIPTAFFETFSDIAAAKAYVEKHGAPIVIKADGLAAGKGVVVAMSKG
                     EAFDAIDMMLSDNKLGDAGARVVVEEFLAGEEASFIVMVDGKNVLTMATSQDHKRLLD
                     NDQGPNTGGMGAYSPAPVVTPEVHARALREVIQPVVRGMESEGIVYTGFLYAGLMISP
                     DGSIKVLEFNCRMGDPETQPIMLRLKSDLANIFEHAVNGTLDQVEAEWDRRTALGVVM
                     AAANYPDTPRKGDVIEGLPKGLEDAHVFHAGTEMQDGKVVTSGGRVLCVVALGDMVKM
                     AQKRAYEIADTIHFDGSQMRRDIGYRAIAPRK"
     gene            4352..4909
                     /locus_tag="VFW59_05645"
     CDS             4352..4909
                     /locus_tag="VFW59_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX5363731.1"
                     /translation="MPRFPASFRRIAAHLRRGGLIAYPTESCYGLGCDPDNRTAVQRL
                     LRLKQRRQRKGLILIAAHFHQVARYLQQLTAAEQLRLSEEGAQAVTYLMPAHHSTPRW
                     LRGAHDTLAVRLTAHPYARKLCRGVNSALVSTSANRSGQRPTKTYAQCQRQFGRQVWV
                     LPGRVGKRKQPSTIRTWADGKIIRK"
     gene            4932..5846
                     /gene="hemF"
                     /locus_tag="VFW59_05650"
     CDS             4932..5846
                     /gene="hemF"
                     /locus_tag="VFW59_05650"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX5363732.1"
                     /translation="MDSNAVKEYLLELQELIVERLEQVDGKKFLRDKWTRSTGSGGIG
                     KGEGISCIIEEGNVLERGGVAFSHVQGDKMPASATAHRPELAGCSWEAMGVSLVMHPR
                     NPYAPTSHANVRMFMAHKPDGEKVFWFGGGMDLTPYYGFEEDAKHFHQVCKNSLAPFD
                     PSLHAKYKKWCDEYFFLKHRNEPRGVGGIFFDDLSTPNFESCFAIQQSVGDHYLSAYV
                     PLLEKRKDTPYGERERDFQLYRRGRYVEFNLVIDRGTIFGLQSNGRTESILLSMPPLV
                     KWRYDWHPEKGTPEAELYDNFLVPKDWI"
     gene            5937..6335
                     /locus_tag="VFW59_05655"
     CDS             5937..6335
                     /locus_tag="VFW59_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363733.1"
                     /translation="MIVDGFRSSLKFYAELIEDGEVDALLLISMDRKLCDVANHIAVC
                     HRIYTERRFEAKTIFSIMSLRRTSLIQDSDRADKMLEETLEIQDKLLALRASVLVRLF
                     ERSNHRHRNEPIARELAEGNEQHGLPRVQA"
     gene            complement(6249..6644)
                     /locus_tag="VFW59_05660"
     CDS             complement(6249..6644)
                     /locus_tag="VFW59_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX5363734.1"
                     /translation="MTETASLISLFVSSFLAATLLPGGSEAVLFGVLKAYPGTLWTAL
                     AVATVGNTLGGMVSFGMGWLLPQTQQLKHVEKVRRYGTPVLLLSWAPLIGDALCLAAG
                     WLRLNPWQAMLFIALGKFARYWLIAMAVI"
     gene            complement(6653..7435)
                     /locus_tag="VFW59_05665"
     CDS             complement(6653..7435)
                     /locus_tag="VFW59_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEX5363735.1"
                     /translation="MSKLNRTVAASLLAVSLQASAFDLGGLLNKEQLGKLGDAVAPST
                     SSPAAQSTDLSMFSNKDQIGSLKQALTQGAETAVMNLAKENGYLGNPKVRIPLPESLQ
                     KADQLLRQFGMGKYADDLTTSMNRAAEAAVPEAKALLVGAVKQMTIADAKTILTGPSD
                     AATQYFRKTTETALAGKFKPIVSKSMAKVQLAQSYDRFAGKGAQFGLVDERDAKLDDY
                     ITRRALDGLFLMMAEQEKAIRANPLEATGNLAKKVFSVLRGQ"
     gene            complement(7441..>8456)
                     /locus_tag="VFW59_05670"
     CDS             complement(7441..>8456)
                     /locus_tag="VFW59_05670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5363736.1"
                     /translation="TFDNAIVAAGSSVARIPGFPYEDERIIDSTGALALKDVPKRMLV
                     IGGGIIGLEMATVYDALGAKISVVELMDQLMPGADKDMVKPLHTRIAKRYEAIMLKTK
                     VTKIEATKAGLKVTFEGEQAPAEPQLYDRVLMAVGRRPNGREIAAEAAGLIVNERGFI
                     PVDKQMRTNVPHIFAIGDIVGDPMLAHKAVHEGKVAAENIAGHKAFFEPLTIPSVAYT
                     DPEIAWMGLTETQAKAQGIEYEKGVFPWAASGRALSIAREEGSTKVLLDPKSRRILGA
                     GIVGVNAGELIAEAVLALEMGADMEDIGLTIHPHPTLSETFNFAAEMAEGTITDLMPP
                     RKR"
BASE COUNT         1702 a         2528 c         2663 g         1563 t
ORIGIN      
        1 tgattaattt ataatcagcc ccccttcgga aacgccctca tgcaaatcgg accatacaaa
       61 ttaaagaaca acctgatcgt cgcccccatg gcgggcgtga cggacaggcc gttccgcatc
      121 ctgtgcacac gtatgggcgc gggcatggcg gtgagcgaga tggtggcgtc gaattccctg
      181 ttatacggct cagagaagac caagcgacgg gcgaatcacg aaggcgaggt cgatccgatt
      241 tcggtgcaga tcgtcggcgc cgatccgaaa atgctggcgc aggccgcgcg ccacaacgtg
      301 gacaacggcg cgcagatcat cgacatcaac atgggctgtc cggccaagaa aatctgcaat
      361 gtgatggcgg gttccgcgct gttgcagaac gagttgctgg tggccgagat actggaagcg
      421 gtggtcgctg cggtggacgt gccggtgacg ctgaagatcc gcaccggctg ggacaggaac
      481 aaccgcaacg cggtgaacat cgcgcgcatc gcggagtcct gcggcattca ggcgctggcg
      541 attcacggcc gcacccgcgc ctgtgcctac acaggcgatg ccgaatacga caccatccgc
      601 gccgtgaagg ccgaggtgaa catccccatc atcgccaacg gcgacatcac cacgcccgag
      661 aaggcgaaat acgtgctgga acacaccggc gtggacgcgg tgatgatcgg ccgtgcggcg
      721 caggggcgtc cctggctgtt ccgggagatc gaatactttt tgcaaaccgg cacgcatctg
      781 ccgtcaccgg aagtcggcga gattcatcgc attctggtcg cgcatgtgca ggagctatac
      841 gacttttatg gcgagcacac cggcctgcgc gtggcgcgca aacacatctc ctggtacacc
      901 aaggggctgg tcggctcggc gcatttccgc catcacatga atcgactgga gacgccctcc
      961 aagcaaatgg cgatggtgga cgaattcttt atagaacaag cgcagagcgg aacgcgactg
     1021 cgctacctgg aggagatggc ggcatgaccg attgtgcagt ggatgagaac gatatggcgc
     1081 gctatgtgcg caaggcgctc aaggattatt tcaaggacct cgacggcgaa gatccttgct
     1141 gcaatatgta cgacatggtg atggattgcg tcgagaagcc gctgatcgag atggtgctcg
     1201 ggcacgtcgg cggaaaccag acccaagcgg ccgcgatgct cggcatcaac cgcaacacgc
     1261 tgcgcaagaa gatgctgcag cacgggatag cgtaacgcac agaataccgt aggggcgcgc
     1321 cgtgccggtg ccggaacaaa agtttcatgt gcccctgccg aacagaattt atttaatcgg
     1381 gaatcatcat ggcagcaatc aagcaagcac tcatcagcgt gtcggacaaa tccggcgtcc
     1441 tcgaattcgc gcaggggctg catagtctcg gcgtgaagct actctctacc ggcggcaccg
     1501 caaagctgct gtccgacaat ggcgtgccct gcaccgaagt cgccgattac accggcttcc
     1561 ccgagatgct cgacggccgc gtgaagacgc tgcagccgaa agtccacgca ggtatcctgg
     1621 cgcgccgcga cctgcccgag catgtcgcca cgctggcgaa gcatggcatc ccgaccatcg
     1681 acctcgtcgt ggtgaatctg tatccgttcg cggcgacggt agcgaagccc ggctgcacac
     1741 tggaagacgc catcgagaac atcgacatcg gcggcccgac catggtgcgt gcggcggcca
     1801 agaaccacgc gcatgtcgcc atcgtcaccg atccatccga ttacacctcg gtgctggccg
     1861 agatgcagac caacgcgggc gcggtcagcg atgcgactcg cttcgatctg atgaagaagg
     1921 cgttctcgca caccgccgcc tacgacagca tgatcagcaa ctacctgacc gcgatcgatt
     1981 ccgacggcgc gaagagcgag ttccccgcgc agatcaattt caacttcgcc aaggtgcagg
     2041 acatgcgcta cggcgagaac ccgcaccaga gcgcggcgtt ctatcgcgac ctgaacccgg
     2101 tggccggcgg catcgccgac tacacccagc tgcagggcaa ggaactgtcc tacaacaaca
     2161 tcggcgacgc cgatgcggcg tgggagctgg tgaagacttt cgatcagccc gcctgcgtga
     2221 tcgtcaagca cgccaatccc tgcggcgtgg cgatcgccga caccgcgctg aatgcctaca
     2281 agctggccta tgcgaccgat accacttccg cgtttggcgg catcatcgcc ttcaaccgcg
     2341 agctggatga ggccacagcc gcgcagatca ccgcgaacca gttcgtcgaa ctgatcatcg
     2401 cgccttccgc gtccgaagcc gcattgaagg tgaccgccgc caagcagaac gtgcgcgtgc
     2461 tcaccgtgcc gctgtccaac gcgcacaacc aatatgacgt gaaacgcgtc agcggcggtc
     2521 tgctggtgca atcgccggat gcgctgaacg tgcagctgtc gcagctgaaa gtcgtcagca
     2581 aggtacagcc gaccgccgat cagctgacgg acctgctgtt cgcctggcgc gtcgcgaaat
     2641 acgtgaagtc caacgcgatc gtgttctgca agggcgggca aactttaggg gttggcgcag
     2701 ggcagatgag ccgcgtggac tcgacccgca ttgccagcat caaggcacag aatgcgggcc
     2761 tgagcctggt gaattctgtc gtgtcttccg acgccttctt ccccttccgc gacggtatcg
     2821 acgtgctggc gcaggccggt gcgaaggccg tgattcagcc gggcggctcg atgcgcgacg
     2881 aggaagtgat cgctgcagcc gatgagcatg gtttggcgat ggtgttcacg ggttaccgtc
     2941 acttccgtca ttaacaaggt aggggcacgg cgcaacaaag tcaggcaatt cactatccag
     3001 caagctggat gtgaaattga caccgccgtg cccctacgcg cggtgtttct cgttttcctt
     3061 ttcacgggtt ttaatcatga aaatattggt tatcggttcc ggcggtcgcg agcacgcgct
     3121 ggcatggcgt ctggcgcagg gcgcgaaggt gcagaaggtg tatgtcgcac cggggaatgc
     3181 aggcacggcg ctggaagagg gcctggagaa tgtcgcgatc accgcgatcc ccgatctgat
     3241 cgaattcgtc aaacgcgaga acgtagagct gacggtcgtc ggcccggaag cgccgctggc
     3301 ggcgggggtg gtggatgcgt tccgcgcggc aggactgaaa atcttcggcc cgaccaaggc
     3361 ggctgcgcag ctggaatcct ccaaggattt cgccaagcgt ttcatgacgc gccataacat
     3421 ccccacggca ttcttcgaga ccttcagcga catcgcggcc gcgaaggctt atgtcgagaa
     3481 gcacggcgcc cccatcgtga tcaaggccga cggcctcgcg gcgggcaagg gcgtggtggt
     3541 ggcgatgagc aagggcgaag ccttcgatgc catcgacatg atgctgtccg acaacaagct
     3601 gggcgatgcc ggcgcgcgcg tggtggtcga ggaattcctc gctggcgagg aagctagctt
     3661 catcgtgatg gtggacggca agaacgtgct gaccatggcg accagtcagg atcacaagcg
     3721 tttgctggat aacgatcaag gcccgaatac agggggcatg ggggcgtatt cgcccgcgcc
     3781 ggtggtcacg cccgaggtgc atgcgcgcgc actgcgcgag gtgatccagc cggtggtgcg
     3841 cggcatggaa tccgagggca tcgtgtacac cggtttcctc tatgccggcc tgatgatctc
     3901 tccggacggc agcatcaagg tgctggaatt caattgccgc atgggcgacc ccgagaccca
     3961 gcccatcatg ctgcgtttga agagcgatct ggccaatatt ttcgagcatg cggtcaacgg
     4021 cacgctggat caggtcgaag cggaatggga ccggcgcacc gcgctgggcg tggtgatggc
     4081 ggcggcgaac tatcccgaca caccgcgcaa gggcgatgtc atcgaaggtc tgccaaaagg
     4141 actggaagac gcgcatgtgt tccatgcggg aaccgagatg caggatggca aggtggtcac
     4201 cagcggcggt cgcgtgctgt gtgtcgtggc gctcggcgat atggtaaaaa tggcgcagaa
     4261 gcgcgcttat gagatcgccg acaccatcca tttcgacggc agccagatgc ggcgcgacat
     4321 cggctatcgc gccatcgcac caaggaagta attgccccgt ttccccgcat cgttccgccg
     4381 catcgccgcg catcttcggc gcggcggcct gatcgcctat ccgaccgagt cctgttacgg
     4441 gctcggttgc gacccggaca atcgtactgc ggtgcagcgt ctgctcagac tcaagcagcg
     4501 ccgccagcgc aagggactga tcctgatcgc cgcgcacttt catcaagtcg cacgctatct
     4561 gcaacagctg accgccgcag agcaactccg gctgagcgag gagggcgcgc aggccgtcac
     4621 ctatctgatg ccggcacatc attcgacacc gcgctggctg cgcggtgcgc atgacacgct
     4681 ggcggtgcgc ctgaccgcgc atccctatgc gcggaaactg tgtcgcggcg tcaacagtgc
     4741 gctggtttcc accagtgcca accgcagcgg gcagcgtccc acgaagacct atgcgcaatg
     4801 ccagcgccag ttcggcagac aggtgtgggt gttgccgggg cgagtcggaa aacgcaagca
     4861 accttcgacg ataagaacat gggcggatgg taaaatcatc cgcaaataac aacgtgatat
     4921 tgggagagaa aatggacagc aacgccgtca aagaatacct gctcgaactg caagagctga
     4981 tcgtcgaacg tctggagcaa gtggacggca agaaattcct gcgcgacaag tggacgcgct
     5041 ccacggggag cggcggcatc ggcaagggcg aaggcatcag ctgcatcatc gaggaaggca
     5101 acgtgctgga gcgcggcggg gtcgctttct cgcatgtgca gggcgacaag atgcccgctt
     5161 ccgccaccgc gcatcgtccc gagctggcag gatgcagctg ggaggcgatg ggcgtgtcgc
     5221 tggtgatgca cccgcgcaac ccctacgcgc cgacctcgca cgccaacgtg cgcatgttca
     5281 tggcgcacaa gcccgacggc gagaaggtgt tctggttcgg cggcggcatg gatctcacgc
     5341 cctattacgg cttcgaggaa gacgcgaaac acttccatca ggtgtgcaag aactcgctgg
     5401 ccccgttcga tccgtcactg catgcgaaat acaagaaatg gtgcgacgaa tatttcttcc
     5461 tcaagcatcg caatgaaccg cgcggcgtgg gcggtatctt cttcgacgat ctgagtacac
     5521 ccaacttcga gagctgcttt gcgatccagc agagcgtggg cgaccactac ctgtcggctt
     5581 acgtgcctct gctggagaag cgtaaagaca cgccctacgg cgaacgcgag cgcgatttcc
     5641 agctctatcg tcgcggccgc tacgtcgagt tcaatctggt tatcgaccgt ggcaccatct
     5701 tcggtctgca atcgaacgga cgtaccgaat ccattctgct gtcgatgccg ccgctggtga
     5761 aatggcgcta cgactggcat ccggagaaag gcacgccgga agcggagttg tacgacaact
     5821 tcctggtgcc gaaagactgg atctgatccc tgaaaacgaa acccgccagc ggcgggtttt
     5881 tgttgtgggt gtcgcatcgc gggtgtattt cacatcgatc gcttaaaagg atagtcatga
     5941 ttgttgatgg attccgttct tccctgaagt tctatgccga attgatcgag gacggtgagg
     6001 tcgatgccct gctgctgatc tcgatggata gaaaactttg cgatgtggcc aatcacatag
     6061 cggtctgcca ccggatctat accgaaagac ggttcgaggc gaagacgata ttttcgatca
     6121 tgtcgctgcg ccggacgagt ttgattcagg actcggatcg cgccgacaag atgctcgaag
     6181 aaacgctgga aatacaggac aaactgctgg cgctgagggc gagtgtgctg gtgcgattgt
     6241 tcgaacggtc aaatcaccgc catcgcaatg agccaatagc gcgcgaactt gccgagggca
     6301 atgaacagca tggcttgcca cgggttcagg cgtagccagc cggcggccag acagagcgca
     6361 tcgccgatca gcggcgccca ggacagcagc agcacgggtg tgccgtagcg gcgcaccttc
     6421 tctacatgtt taagctgttg ggtctgcggc agcaaccagc ccatgccaaa gctgaccatg
     6481 ccgcccagcg tgttgccgac tgtggcgacg gcgagcgcag tccacagcgt acccgggtaa
     6541 gccttgagca cgccgaacag caccgcctcg gagccgccgg gcagcagcgt tgcggcgaga
     6601 aaactgctga cgaacagcga gatgaggctg gcggtttcgg tcacagacga atctattgac
     6661 cgcgcagaac cgagaatact ttcttcgcca gattgccggt cgcttccagc ggattcgcgc
     6721 ggatggcttt ctcctgctcg gccatcatca ggaacaggcc gtcgagcgca cggcgggtga
     6781 tgtagtcgtc cagcttcgca tcgcgctcat ccaccagtcc gaactgcgcg cccttgcccg
     6841 cgaagcggtc atagctctgt gccagttgca cctttgccat cgacttgctg acgatgggtt
     6901 tgaacttgcc cgcgagtgcg gtttcggtgg tcttgcggaa gtattgcgtc gcggcgtcac
     6961 tcgggccggt gagaatggtt ttcgcatccg cgatggtcat ctgcttcacc gcaccgacca
     7021 gcagtgcctt tgcctccggt acggcagctt cggcggcgcg gttcatcgag gtggtgaggt
     7081 cgtcggcata cttgcccatg ccgaactggc gcagcagctg gtcggctttc tgcaggctct
     7141 ccggcagcgg gatgcgcacc ttggggttgc cgagatagcc gttctccttt gccagattca
     7201 tcaccgccgt ttccgcaccc tgagtcagcg cctgcttcag gctgccgatc tgatccttgt
     7261 tgctgaacat ggacaggtcg gtggactgtg cggcaggaga agatgtgctc ggcgcgacgg
     7321 catcgccgag cttgcccagc tgctccttgt tcagcagccc gcccagatcg aacgcgctgg
     7381 cctgcagtga aacggcgagc agcgaggctg cgacggtgcg attcagtttt gacatgtggc
     7441 tcagcgcttg cgcggcggca tcaggtcggt gatcgtgcct tcggccattt cggcagcgaa
     7501 gttgaacgtc tccgacaggg tcgggtgcgg gtggatcgtc agaccgatgt cttccatatc
     7561 cgcgcccatc tccagcgcga gcaccgcctc ggcgatcagc tcgccggcat tcacgccgac
     7621 gatgcccgcg ccgaggatgc ggcgcgactt gggatcgagc agcaccttgg tcgaaccttc
     7681 ttcgcgtgcg atggacagcg cgcgaccgga ggcggcccag gggaagacac ccttctcgta
     7741 ttcgataccc tgtgccttcg cctgtgtctc ggtcaacccc atccatgcga tctcggggtc
     7801 ggtgtaggcg accgacggga tggtcaacgg ctcgaagaat gccttgtgcc cggcgatgtt
     7861 ctccgccgcg accttgccct catgcacggc cttgtgcgcc agcatcgggt cgccgacgat
     7921 gtcgccgatc gcgaagatgt gcggcacgtt ggtgcgcatc tgtttatcga cggggatgaa
     7981 gccgcgctcg ttgacgatca ggcccgccgc ttcggccgcg atctcgcgcc cgttcggacg
     8041 gcggcccacc gccatcagca cgcggtcgta gagttgcggt tcggcaggcg cctgttcgcc
     8101 ttcaaaggtg actttcaggc cggccttggt cgcttcgatc ttggtgacct tggtcttcag
     8161 catgatggct tcgtaacgct tggcgatgcg ggtgtgcaac ggcttgacca tgtccttgtc
     8221 ggcaccgggc atcaactgat ccatcagctc gaccacggaa atcttcgctc ccagcgcgtc
     8281 gtataccgtc gccatttcca gaccgatgat gccgccgccg atgaccagca tgcgcttcgg
     8341 cacatctttc aatgccagtg cgccggtgga gtcgatgatg cgctcgtctt cataggggaa
     8401 gccgggaatg cgcgcgacgc tggaaccggc tgcgacgatg gcgttgtcga aagtga
//
LOCUS       DASUIV010000151         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_36202, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000151 DASUIV010000000
VERSION     DASUIV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36202"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFW59_05675"
     CDS             complement(<1..268)
                     /locus_tag="VFW59_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363737.1"
                     /translation="MKRLHILLLISSLLAVGQAQVCAAEQKFNAKVIAVLDGDTLLVT
                     RGTKPLRLRLAEIDAPEVAHAGPGDRAPNQQPYGTASQKSLAELV"
     gene            complement(265..768)
                     /locus_tag="VFW59_05680"
     CDS             complement(265..768)
                     /locus_tag="VFW59_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX5363738.1"
                     /translation="MTTSDELIVVPDWRFLLSHPAHLLSFGFGSGLARRAPGTFGTLV
                     AFPIYWYLAPRLSDALFILVLIWAFAIGVWVCDITGRALGVPDYGGIVWDEIVAFLLV
                     LFFTPPGWEWSLLAFALFRFFDIVKPPPIRYFDSNWHGGLGVMFDDLLAAGYALLCLA
                     IIKTFLS"
     gene            complement(752..1696)
                     /gene="thiL"
                     /locus_tag="VFW59_05685"
     CDS             complement(752..1696)
                     /gene="thiL"
                     /locus_tag="VFW59_05685"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX5363739.1"
                     /translation="MSEFDLIRRHFTRPTSNTLLGVGDDAALLQPSAGKVLAVSSDML
                     VSGTHFFADADPYWLGHKTLAVNLSDLAAMGATPRWATLAIALPAADEVWLEQFSAGF
                     FALAQRYGVELVGGDTTRGPLNLCVTIFGEVPPEQALRRDGAQSGDEIWVSGQLGGAA
                     LALAHLQGRTVLSDADLAVCMPRLHQPQPRIALGLALRGIAHSAIDVSDGLLADLGHI
                     LEASRVGAEIEFDSIPAEFDGHDEPLLQRCILAGGDDYELCFTVPAARHAEVERLAAQ
                     LGLPLTRIGKIVAQCGCLVQDAAGNTIDIEAAGYDHFR"
     gene            complement(1748..>2083)
                     /gene="nusB"
                     /locus_tag="VFW59_05690"
     CDS             complement(1748..>2083)
                     /gene="nusB"
                     /locus_tag="VFW59_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5363740.1"
                     /translation="HTLGEKNLGRYDKPMFSRLLRGALAQHESVEALLTPHLDRPMSE
                     LSPVEFSVLLLGAFELSQHLEVPYKVVINEAVELAKTFGGTDGHKYVNGVLDKLAPLV
                     RAVEFAARK"
BASE COUNT          467 a          685 c          605 g          326 t
ORIGIN      
        1 tcaccagttc cgccagcgac ttttgcgaag ccgtaccgta tggctgctgg ttgggtgccc
       61 ggtcgccagg ccctgcatga gccacttccg gcgcatcgat ctcggccagt cgcagcctca
      121 gcggcttggt gccgcgcgtg accagcagcg tgtcgccatc cagcacggcg atgaccttgg
      181 cattgaattt ctgctcggca gcgcatacct gtgcctgccc caccgcaaga agcgacgaga
      241 tcagcaacag gatatgcagc cgcttcatga cagaaaggtc ttgatgatgg cgagacagag
      301 cagcgcgtaa ccggctgcca gcaggtcgtc gaacatcacc cccagcccgc cgtgccagtt
      361 gctgtcgaaa tagcggatcg gcggcggttt gacgatatcg aagaaacgga acagcgcgaa
      421 ggccagcagc gaccattccc agcccggcgg cgtaaagaac agcaccagca gaaaggcgac
      481 gatctcgtcc cacacgatgc cgccgtaatc gggcaccccc agcgcacggc cggtgatgtc
      541 acatacccat acgccgatgg cgaatgccca gatcaacacc aggatgaaga gtgcgtcgga
      601 cagacgcggc gcgagatacc agtaaatcgg gaaggcgacc agcgtgccga aggtgcccgg
      661 cgcgcgacgc gccagtccgc tgccgaaacc gaacgacagc agatgcgccg ggtgactgag
      721 cagaaagcgc cagtccggca cgacgattaa ttcatcggaa gtggtcatag cccgcagcct
      781 cgatatcgat agtattaccg gcggcatcct gcaccagaca gccgcattgc gcaacgattt
      841 tcccgatgcg ggtcaacggc agaccgagct gcgcggcgag tcgttcgacc tcggcatgac
      901 gcgcggctgg cacagtaaaa cacagctcgt aatcgtcgcc gcctgccaga atgcagcgct
      961 gcaacagcgg ctcatcatga ccgtcaaatt ctgccgggat cgaatcaaat tcgatttccg
     1021 cccccacccg cgaggcctcg agaatatgtc cgagatcggc gagcaaaccg tccgacacat
     1081 cgatcgcgct atgggcgata ccgcgcaacg ccaggcccag tgcgatacgt ggctgcggct
     1141 ggtgcagacg cggcatacaa acggccaaat cggcatcgga caacacggtg cgcccctgca
     1201 aatgcgccaa cgccaacgca gcaccgccca gttggccgga cacccagatc tcgtcgccgg
     1261 actgcgcgcc atcgcgccgc aatgcctgct ccgggggcac ctcgccgaag atggtcacgc
     1321 acagattcag cggcccgcgc gtcgtatccc cgccgaccag ctccacgcca taccgctgcg
     1381 ccagcgcaaa gaagcccgca ctgaattgct ccaaccagac ctcatccgct gcaggcagcg
     1441 caatcgccag cgtcgcccag cgcggtgtcg ctcccatcgc ggccagatcg gacagattca
     1501 ccgccagcgt cttatgcccg agccaatacg gatcggcatc cgcgaagaaa tgcgtgccgc
     1561 tgaccagcat atcggatgag accgccagca ccttgcccgc cgaaggttgc agcagcgcgg
     1621 catcgtcgcc gacacccagc agggtattcg atgtcgggcg ggtgaaatgg cggcggatca
     1681 gatcgaattc ggacattgga aaattcggca ggatgggttg agcgcagcga tatccatcct
     1741 acaaatatta tttgcgtgcc gcgaattcca cggcgcgcac caatggcgcc agcttgtcga
     1801 gcacaccgtt gacatatttg tggccgtcgg taccgccgaa ggtcttggcc aactcgaccg
     1861 cctcgttgat gaccaccttg taaggcactt cgagatgctg cgacaactcg aatgcaccga
     1921 gcagcaacac ggaaaattcc accggactga gttcggacat cggacgatcc agatgcggag
     1981 tcaacagcgc ctcgacgctc tcgtgctgcg ccagcgcgcc gcgcaacaac cgggagaaca
     2041 tcggcttatc gtaccgaccg agattctttt caccgagagt atg
//
LOCUS       DASUIV010000152         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_129354, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000152 DASUIV010000000
VERSION     DASUIV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129354"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1470
                     /locus_tag="VFW59_05695"
     CDS             94..1470
                     /locus_tag="VFW59_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEX5363741.1"
                     /translation="MNSIDQTLPVVVIGGGPAGLMAAETLAQLGMRVELYDAMPSVGR
                     KFLMAGKGGMNITHSEPLEILLSRYGTRRAQLEAMLRTFPPEALREWMRGLGIESFIG
                     SSGRVFPEGMKAAPLLRAWLHRLREQGVRFHVRHRWCGWDAEGALRFATPQGEVLVRV
                     AAQGPLLNPLPQSGEGANARIINASGVVLALGGGSWPQLGSDGAWLPLLAQRGIGIAP
                     LRPANSGFEVNWSPHFRERYAGEPLKTVAASFGDERKSGECLITELGIEGGLIYALSA
                     ALRDQIERAGYAMLQLDLLPDWTLQRVQAEVAHPRGSRSLSSHLQSRLGLKGVKANLL
                     REVLSADEMNDPLLLAATIKALPLRLLATRPLIEAISSAGGVPFETLDENLMLRELPG
                     VFCAGEMLDWEAPTGGYLLTACFASGRAAGLGMLDWLGNDFRACARRAGPDSSPAHNP
                     RPSPVSPP"
     gene            complement(1391..3574)
                     /locus_tag="VFW59_05700"
     CDS             complement(1391..3574)
                     /locus_tag="VFW59_05700"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX5363742.1"
                     /translation="MTLPASTAPLGLEDLRYQGLVRFASLLESQTRSRLFTRNIGDLI
                     QSINAEIAENLDDCIRQLQKTGLDPDEKQRLIDLLDISRTRSSRMNLTMELLLKERNR
                     QWEKSEDNLKQIMLDFSGTVDNLAHTLIEKDLFERQSTVLESIILSHEKVAQWKLFVQ
                     EILSGFHSIFPFNFFFIAFAEEHGLSLFVYFLGEYSDEVKQQVRDQLVRDTLATLNLP
                     VDAPLDIEEFQIAASGRYDSIDDIKMITVPVPELEMLNLAGMLGVAYGSLNKLSAQEA
                     SVIRSILAVMVMVVGSSKALSRTLSELEYYSTHDPLTGLYNRRYFNEMLDYEIGRSER
                     HGHEFSILMLDLDDFKDINDTYGHPCGDTALIGVADALHNVVRKGDLATRIGGDEFAI
                     ILTETNKEGGMIVAEKLRSELRDVTFSAPNDKSFHITTSIGIVTYPQDAKNLSDLMTG
                     VDIGLYRAKKLGKDTVCSIDQVETQIQENRVIRDQAESLRNSLSEGRIVPFFQPIIDC
                     KTGKVFAYETLARLNESDGSTVSAAMFIETIEKYGLGRELDRIMIGNGLKAMKEKIRN
                     GDPDIKLFINLSAQEIQGRGILGYAEQMCQEIGIPPKNVVFELLERDAVGDMTHMRKF
                     LTDLRKKGFSFALDDFGSGYNSFHYLRELHFDFVKLDGSFVRNILNSRIDHALVSNLS
                     RLCQDLGILMVAEYVESKEIMEAIQAMGVDYAQGYYLGLPASHMP"
     gene            complement(3576..>4360)
                     /locus_tag="VFW59_05705"
     CDS             complement(3576..>4360)
                     /locus_tag="VFW59_05705"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5363743.1"
                     /translation="GGSRMRNIVLYGSEHHKFVLLNESEPGEEDGVRSNQYLVTHDGC
                     GVLLDPGGFGVMPRVLAEMLRYISPDQLKGIVLSHQDPDIVGGIATWLEITDAEVYVS
                     RIWTRFLPHYGISGMHRFIGVPDEGMPYPVCPGFDLQLVPAHFLHSEGQINVYDPVSK
                     ILFTGDIGAAMLPLEQDYSYVDDFAAHLPYIEEFHRRYMCSNKAARLWVEAVSRLDIE
                     MIAPQHGPVYRGQAVQDFLKWFGELQCGVDLMVSSGQFKAGA"
BASE COUNT          816 a         1241 c         1394 g          909 t
ORIGIN      
        1 gggcgatgcc tgcgtgatac cggcgggatt caacggcacc ttcgaggtgc tggagccggt
       61 gaaaaaacac tacatgatgg tcaagcgcaa ggcatgaatt caatcgatca gactttgccg
      121 gttgtcgtga tcggcggcgg acccgccggg ctgatggcgg cggagacgct ggcgcaactc
      181 gggatgcgcg tcgaactcta cgacgccatg cccagcgtgg gacgcaagtt cctgatggcg
      241 ggcaagggcg gtatgaacat cacgcattcc gagccgctgg aaatcttgct gtcgcgctat
      301 ggcacgcgtc gcgcgcagct tgaagcgatg ctgcgcactt tccccccgga ggcgctgcgg
      361 gaatggatgc ggggactcgg catcgagagt tttatcggca gttccggacg cgtgtttccc
      421 gaggggatga aggccgcgcc gctgctgcgc gcctggctgc accgtctgcg cgagcagggt
      481 gtgcgctttc atgtgcggca tcgctggtgc ggatgggatg ccgagggcgc gctgcgcttc
      541 gccacgccgc agggcgaagt cttggtgaga gttgctgcgc aaggccctct cctcaaccct
      601 ctcccgcaat cgggcgaggg ggcgaacgcg agaatcatca atgcttctgg cgtggtgctg
      661 gcgctgggcg gcggcagctg gccgcaactc ggctcggatg gcgcgtggct gccgctgttg
      721 gcgcagcgcg gaatcgggat cgcgccgctg cgtccggcca actcgggctt cgaggtgaac
      781 tggagtccac actttcgcga acgctacgcc ggtgagccgc tgaaaaccgt cgccgccagt
      841 ttcggtgatg agcgcaaatc gggcgaatgc ctgatcacgg agctgggcat cgaaggcgga
      901 ctgatctatg ccttgtctgc cgcgctgcgc gaccagatcg agcgcgcggg ctatgcgatg
      961 ctgcagctcg acctgctgcc ggactggacc ttgcagcggg tgcaggccga ggtggcgcat
     1021 ccgcgcggtt cgcgttcgct gtccagccac ctgcaaagcc gcctcggtct gaagggggtg
     1081 aaggccaatc tgttgcgcga agtgctgagc gccgacgaga tgaacgaccc tctgttgttg
     1141 gcggccacca tcaaggcgct gccgctgcgt ctgctcgcga cgcgtccgct gattgaggcg
     1201 atcagcagcg cgggcggcgt gccgttcgag acgctggatg aaaacctgat gctgcgcgaa
     1261 ctgcccggcg tgttctgtgc gggcgagatg ctggactggg aagcgccgac cggcggttac
     1321 ctgctcacgg cctgtttcgc gagcggccgg gcggcggggc tgggcatgct ggactggctg
     1381 gggaatgact tcagggcatg tgcgaggcgg gcaggcccag atagtagccc tgcgcataat
     1441 ccacgcccat cgcctgtatc gcctccatga tttccttgga ctcgacatat tcggcaacca
     1501 tcaaaatacc cagatcctga cataagcggc tgaggttgct gactaacgca tggtcgatcc
     1561 tggagttcag tatgttgcgt acgaaggagc cgtccagttt gacgaagtcg aagtgcagtt
     1621 ctcgcaggta atggaatgag ttgtagccgc tgccgaaatc gtccagtgcg aaggagaaac
     1681 ctttcttgcg cagatcggtg agaaacttgc gcatatgcgt catgtcgccg acggcatcgc
     1741 gctccagcag ctcgaagacg acgtttttcg gcggtatgcc gatctcctgg cacatctgtt
     1801 ccgcatagcc cagaatgccg cgtccctgaa tttcctgtgc cgacaggttg atgaacaact
     1861 tgatgtcggg gtcgccgtta cggatctttt ctttcatcgc cttcagccca ttgccgatca
     1921 tgatgcgatc cagttcccgg cccaggccgt atttctcgat ggtctcgatg aacatcgcgg
     1981 cagagaccgt gctgccgtcc gattcattca ggcgcgccag cgtctcatag gcgaaaacct
     2041 tgccggtctt gcagtcgatg atgggctgaa agaacggcac gatacgacct tcgctcaggg
     2101 agttgcgcaa tgactctgcc tgatcgcgga tgacgcgatt ctcctgaatc tgcgtttcca
     2161 cctgatcgat cgagcagacg gtgtctttgc cgagtttctt ggcgcggtac agcccgatat
     2221 cgacaccggt catcaggtcg gagaggttct tggcatcctg cgggtaggtg acgataccga
     2281 tcgacgtggt gatgtgaaag ctcttgtcgt tcggcgcgga gaaagtcaca tcgcgcaatt
     2341 cggagcgcag cttctcggcg acgatcatgc cgccttcctt gttcgtttcc gtgaggatga
     2401 tggcgaattc gtcgccgccg atgcgcgtgg cgagatcgcc ttttcgcacc acgttatgca
     2461 aggcatctgc gacgccgatc agcgcggtat cgccacaggg gtggccgtag gtgtcgttga
     2521 tgtccttgaa gtcgtcgagg tcgagcatca ggatggaaaa ttcgtggccg tggcgctcgg
     2581 aacgtcctat ctcgtagtcg agcatctcgt tgaaatagcg ccggttgtac aggccggtca
     2641 acggatcgtg ggtcgagtag tattccagct cggacagggt gcggctcagc gccttgctcg
     2701 aaccgaccac catcaccatc accgcgagga tggagcggat gacgctggct tcctgagcgc
     2761 tgagcttgtt gagcgagccg taggccacac cgagcatgcc ggcgaggttg agcatctcca
     2821 gttccggcac cggtacggtg atcatcttga tgtcgtcgat ggagtcgtat cgccctgagg
     2881 ctgcgatctg gaattcttcg atgtccagcg gcgcgtcgac cggcagattg agcgtggcta
     2941 aggtgtcacg caccagctga tcgcgtacct gctgtttgac ctcgtcgctg tactcgccca
     3001 gaaaatagac gaatagcgac aggccgtgtt cctcggcaaa ggcgatgaag aagaaattga
     3061 acgggaagat cgagtggaag ccggacagga tctcctgcac gaacagtttc cattgcgcga
     3121 ccttctcgtg cgacaaaatg atgctttcca gcacggtgct ttgccgctcg aacagatcct
     3181 tttcgatcag ggtgtgggcc agattgtcga cggtgccgct gaagtcgagc atgatctgct
     3241 tcaggttgtc ctcgctcttc tcccattgcc ggttgcgttc cttcagcagc agttccatgg
     3301 tgagattcat gcgcgaagag cgtgttctgc tgatgtccag cagatcgatc agccgttgtt
     3361 tctcgtcggg atcgaggccg gttttttgca gttgccggat acagtcgtcg agattctccg
     3421 cgatctcggc gttgatgctt tgtatcaggt cgccgatatt ccgggtgaac aggcgcgagc
     3481 gtgtctggct ctccagcaac gaggcgaaac gaaccagtcc ttgataacgc aggtcttcca
     3541 ggcccaaggg agcggtggat gcgggcagtg tcatctcagg ctccggcctt gaactgtccg
     3601 ctggagacca tcaggtcgac gccgcactgc aattcgccga accatttcag aaaatcctgc
     3661 acggcctgac cgcgatagac cgggccatgc tgcggtgcga tcatctcgat atccagtctg
     3721 gaaaccgcct cgacccacag ccgggcggcc ttgttggaac acatgtagcg gcgatggaat
     3781 tcttcgatgt agggcaggtg tgccgcgaag tcgtcgacat aggaataatc ctgttccagc
     3841 ggcagcatgg cggcaccgat gtcgccggtg aacaggattt tcgagaccgg gtcgtaaacg
     3901 ttgatctgcc cctcggagtg cagaaagtgc gcgggaacca gttgcaggtc gaaccccgga
     3961 cagaccggat agggcatgcc ctcatccggc acgccgatga aacggtgcat gccgctgatg
     4021 ccgtaatggg ggaggaagcg ggtccagatg cgcgagacgt aaacctccgc atcggtgatt
     4081 tccagccagg tggcgatacc gccgacgatg tccggatcct gatgggacag gacgataccc
     4141 ttgagttggt ccggggagat gtagcgcagc atctccgcca gcacgcgcgg catcacgccg
     4201 aaaccgcccg gatccagcaa cacgccgcaa ccgtcgtggg tcaccagata ctggttggac
     4261 ctgacaccgt cttcttctcc cggctcgctc tcgttcaata atacgaattt gtgatgttcc
     4321 gagccataca agacgatgtt gcgcatgcga cttccccctg
//
LOCUS       DASUIV010000153         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_780028, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000153 DASUIV010000000
VERSION     DASUIV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780028"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1108)
                     /gene="moaA"
                     /locus_tag="VFW59_05710"
     CDS             complement(116..1108)
                     /gene="moaA"
                     /locus_tag="VFW59_05710"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5363744.1"
                     /translation="MNAVMQDGFGRQIDYLRMSVTDRCDFRCVYCMAKNMTFLPRQQV
                     LTLEELQRLATLFVGLGVRKIRLTGGEPLIRPGIVDLCRNIAALPGLRELVMTSNGSQ
                     LGRLARPLVDAGVKRMNISLDSLDGQKFRAITRIGDLDQVLNGIEAARDAGFERIKLN
                     CVVMKGRNFDEVPALVQYAIDQRIDISFIEEMPLGDVGRSRGESFCSSDEVRALIASQ
                     HRLLDSTENSGGPARYVRLERHPETRIGFISPNSHNFCGSCNRVRMTVEGKLLLCLGQ
                     DDALDLRGLLRRYPLDDQPVINAVQKALRGKPLRHDFSPEGEVQIVRFMNMSGG"
     gene            complement(1134..>2012)
                     /locus_tag="VFW59_05715"
     CDS             complement(1134..>2012)
                     /locus_tag="VFW59_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5363745.1"
                     /translation="DIAPVAAAQFEALPVEPAAADDAPAQLIASSEQEWPIISVNEVS
                     ITPEAMALELQYHPAESREEAVYLAARALVIRELLQQRIAELGVSMEIGAGENEEEAA
                     TRLLLEREVHVPQCDEETSLRYYENNRGRFHSAPLLAVRHILLECAPDDVEARSLAHV
                     QAEVLLQRLDEFPGTFAELALKYSACPSKAQGGSLGQISKGQTVPELERQLFTLAPGL
                     ASKPLESRYGWHVVSVDQRIEGMPLPYEVVSTAIRTQLQQGVWQKALVQYLQTLIGAA
                     DIRGIHLQGADSPLVQ"
BASE COUNT          405 a          656 c          545 g          406 t
ORIGIN      
        1 tgggcgcgag cctgctcgcg atgactacag cacgctcaac attgatgtcg attgttctac
       61 ggctatcgcg agcaggctcg ctcccacagt gatttgctgt gttttacaga tcagctcaac
      121 cgccactcat gttcatgaac cgtacaatct gcacctcccc ttcggggctg aaatcgtggc
      181 gcaacggttt gccgcgcaag gccttctgca cggcattgat cacgggctga tcatccagcg
      241 gataacgccg cagcaatcct cttaaatcca gggcatcgtc ctgccccaaa cacagcaaca
      301 gcttcccttc aacggtcatc cgtacccggt tgcagctgcc gcagaagttg tggctgttgg
      361 gtgaaatgaa gccgatccgg gtctcgggat ggcgttccag acgcacatag cgtgccggtc
      421 cgccgctgtt ttcggtgctg tcgagtaacc gatgctggct ggcgatcagc gcccgaacct
      481 cgtcgctgga acaaaacgat tcaccccggg aacgcccgac atcgcccaag ggcatttcct
      541 cgatgaaact gatatcgatg cgctggtcaa tggcgtactg caccaacgcc gggacttcat
      601 caaagttgcg gcccttcatc accacgcagt tgagtttgat ccgctcgaac cccgcgtccc
      661 gtgctgcctc aatgccgttg agcacttgat cgagatcgcc aatgcgggtg atcgcgcgaa
      721 acttctgccc gtccaggcta tcgaggctga tgttcatccg cttcaccccg gcatcgacca
      781 acggccgggc cagacggccc agctgcgagc cgttgctggt catcaccaat tcgcgcaaac
      841 cgggcaacgc ggcgatgttg cgacaaaggt cgacaatgcc cggacgaatc agcggctcgc
      901 cgccggtcag gcgaatcttg cgtacaccca gaccaacgaa cagcgttgcc aaacgctgca
      961 actcttcaag ggtgagcacc tgctgacgcg gcaggaaggt catgtttttc gccatgcaat
     1021 acacacagcg aaaatcacag cggtccgtca ccgacatccg cagataatca atctgccgtc
     1081 caaagccatc ctgcattaca gcgttcatca catctcccgg tttgcctcga tgctcactgc
     1141 accagcggtg agtcggcgcc ctgcaagtgg ataccgcgaa tatccgccgc accgatcagg
     1201 gtttgcaggt actgcaccag ggctttttgc cacacgcctt gctgcaactg cgtgcggatc
     1261 gccgtcgaca ccacttcata gggcaacggc atgccttcga ttcgctgatc gacactgact
     1321 acatgccagc cgtagcgact ttccagcggt ttgctggcca ggcccggcgc cagagtgaac
     1381 aactgacgtt ccagttcagg cacggtttgg cccttgctga tctgccccaa agaaccaccc
     1441 tgagccttcg acgggcaggc cgaatatttc aacgccagtt cggcgaaagt gccggggaat
     1501 tcatccagac gttgcagcag gacttccgcc tgaacatgcg caaggctgcg cgcctcgaca
     1561 tcgtccggcg cacattcgag caggatgtgc cgtaccgcca gcaatggtgc gctgtgaaag
     1621 cgcccgcgat tgttttcgta gtaacgaaga ctggtttcct cgtcgcactg cggcacatgc
     1681 acctcgcgtt caagcaacaa acgcgtggcc gcttcttctt cgttctcgcc agcaccgatc
     1741 tccatcgata cgccgagttc ggcaatgcgc tgctgcaaca actcgcggat caccaacgcc
     1801 cgggcggcca agtacaccgc ttcttcgcgg ctttcggccg gatgatattg cagctccagc
     1861 gccatcgctt ccggggtgat cgacacctcg ttgacgctga tgatcggcca ctcctgttca
     1921 ctgctggcga tcaactgcgc cggggcgtca tcggcagcgg cgggctctac cggcagcgct
     1981 tcgaactgcg ccgctgcgac cggcgcgatg tc
//
LOCUS       DASUIV010000154         4722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_780204, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000154 DASUIV010000000
VERSION     DASUIV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780204"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..859)
                     /gene="nifM"
                     /locus_tag="VFW59_05720"
     CDS             complement(11..859)
                     /gene="nifM"
                     /locus_tag="VFW59_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifM"
                     /protein_id="HEX5363746.1"
                     /translation="MPQVIESGVAYLALKAAHKLYGKAPDTLPSAEFERVQMMAQQQH
                     QLEAQILVAAEARDVMVPPSTVNAAMQEIRGRYQNEEEFNQDLARNGLDQAVFAQALE
                     RELKVEAILEKVGSRAAHIGDMDVELYYLYHPDQFRRPETRLARHILITINDAIAENT
                     RSAAYQRASEIAARLAKEPQRFEEQALKHSECPTALEGGKLGDLPRGKLFPTLDQALF
                     ELNAGEVSGVLESELGFHVLRCDAITAANVLSQSQAKPHIRKLMEQKRKRVCQQGWIK
                     KLLENE"
     gene            complement(843..1319)
                     /locus_tag="VFW59_05725"
     CDS             complement(843..1319)
                     /locus_tag="VFW59_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HEX5363747.1"
                     /translation="MLPKYEFGEEVRIIRNVRNDGTYPGVPTGTLLIRRGSTGFVMNV
                     GTFLQDQLIYTVNFLDQNKIVGFREEELIPIAEQWIPSKFESREKVRSKVTLAVRGEV
                     RATPGSEGEILKVLRDETGGVQYQVIFHDHVLQVPESALEAMHVYHDEEKCDATSH"
     gene            complement(1309..1644)
                     /locus_tag="VFW59_05730"
     CDS             complement(1309..1644)
                     /locus_tag="VFW59_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HEX5363748.1"
                     /translation="MDELTLDEALEELSSAEDFLNYFGIEFEPSVVHVNRLHILQRYH
                     DYLGKTTLPEVEAARRVVYTTLLRQAYQDFEQSDALTEKVFKVFRMHEPQVKFVPLSS
                     ITVNKQDVA"
     gene            complement(1632..2159)
                     /locus_tag="VFW59_05735"
     CDS             complement(1632..2159)
                     /locus_tag="VFW59_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363749.1"
                     /translation="MDFEQAELYFDEPVSAAVGRLLDTAANLYGSDEAELMLLRANFM
                     APQNLTVLVALYRYYFYQHRLEDALLVAESALAIVGRRLEFPDTWVYLAEANVGAGVM
                     RSMGLVRFYLMVLKAAGYTNLRLGNIPTGRAMLEKLVELDSHDRMGGKALLDVVRSQS
                     INNEEIFERSTRWTS"
     gene            complement(2170..2898)
                     /gene="cysE"
                     /locus_tag="VFW59_05740"
     CDS             complement(2170..2898)
                     /gene="cysE"
                     /locus_tag="VFW59_05740"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX5363750.1"
                     /translation="MLKLLREDVSCVFQRDPAARSRLEVLTTYPGVHAILMQRIAHRL
                     WKFGLCFPARILSWFARLLTNIDIHPGATIGRRFFIDHGAGVVIGETAEIGDDVTLYH
                     GVTLGGTTWNKGKRHPTLGDGVVVGAGAKILGAITLGEQVRVGANSVVVKDVPSHRTV
                     IGIPGKIVLRTEEANADNIYGINLDHHLIPDPVGRAIACLMERIEMLEAKLHRQGTAV
                     EGQCRTCEADELCGTEVARTIRKE"
     gene            complement(2956..4101)
                     /gene="nifV"
                     /locus_tag="VFW59_05745"
     CDS             complement(2956..4101)
                     /gene="nifV"
                     /locus_tag="VFW59_05745"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEX5363751.1"
                     /translation="MSRAIIIDDTTLRDGEQTAGVSFTLDEKLAIARQLDAMGVPELE
                     VGIPAMGEEERDSINAVAGLGLKAKLIVWNRMCLEDLVVCEQVDVKLVDLSIPVSDIH
                     LKKKLGRDRQWVLDTIYDIVPRAYHFGRDVIVGCEDASRADPEFMLQVAEAAQAAGAQ
                     RIRFADTLGVMEPFGVHKAIAALRQAVDIEIEMHAHDDLGLATANSLAAAVAGATHIN
                     TTVNGLGERAGNAPLEEVALGLNKLYQLQTGIDLSNFPELSEKVAAASGRPVHWQKSL
                     VGEGVFTHEAGIHVDGMLKDPETYQGFDPSEVGRRHKLVLGKHSGTHGIVAAYATMGL
                     FLSPVEARALLPDIRAFAERAKRSPADQELLSLYQRYIGRNQPGMAH"
     gene            complement(4112..>4722)
                     /locus_tag="VFW59_05750"
     CDS             complement(4112..>4722)
                     /locus_tag="VFW59_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5363752.1"
                     /translation="HKLHAPKGIGVLYLKRGVRFRPLLRGGHQERGRRAGTENTTSIV
                     GLGKAAEMALEAMAFENTEVARLRDKLEAGILARVPRSFVTGNPDDRLPNTSNIAFEF
                     IEGEAILLLLNKFGIAASSGSACTSGSLEPSHVMRAMGIPYTAAHGTVRFSLSRYSTE
                     DEVDRVIQVVPEIIAQLRKLSPYWDGDGPAAEPEKAFAPTYA"
BASE COUNT          891 a         1525 c         1336 g          970 t
ORIGIN      
        1 acattcagga ctattcgttc tccagcaact tcttgatcca gccctgctga cacacgcgtt
       61 tgcgcttctg ctccatcagc ttgcggatat gcggtttggc ttgagactgg ctcaagacgt
      121 tcgctgcggt gatggcgtcg caacgcaaca cgtggaagcc caattctgac tcgagcaccc
      181 cgctcacctc gcccgcattc agctcgaaca atgcctggtc cagcgtcggg aacagcttgc
      241 cgcgcggcag gtcgcccagc ttgccgcctt ccagtgcggt ggggcattcg gaatgtttca
      301 gcgcctgctc ctcgaagcgt tgcggctctt tcgccagccg ggctgcgatc tcgctggcgc
      361 gctggtaggc ggcgctacgc gtgttttccg cgatcgcatc gttgatggtg atgaggatgt
      421 gccgcgccag ccgggtctca ggccgacgaa actgatcggg gtgatagagg taatacagtt
      481 ccacatccat atcgccgatg tgtgcggcac gcgagccgac cttttcgagg atggcctcga
      541 ccttgagttc gcgctccagt gcctgggcaa acacagcctg atccagaccg ttcctcgcca
      601 gatcctggtt gaactcctcc tcgttctgat agcgaccgcg tatctcctgc atggcggcgt
      661 tcaccgtgga aggcggtacc atcacgtcgc gggcttcggc agcaacaagt atctgcgctt
      721 caagctgatg ctgttgctgc gccatcatct gcacgcgctc gaactctgcg gatggcaagg
      781 tgtccggcgc cttgccgtaa agcttgtgcg ctgccttgag cgcgagatag gccacgccgg
      841 attcaatgac ttgtggcatc gcacttctcc tcatcgtgat agacatgcat agcttccagc
      901 gcggattcag gcacttgcag cacatgatca tggaagatca cctgatactg cacgccgccc
      961 gtttcatcgc gcagcacttt caatatttcc ccttcgctgc ccggtgtggc acgcacttca
     1021 ccacgcactg ccagagtgac tttgctgcgc accttttcgc gactctcgaa cttgctgggt
     1081 atccactgtt cagcgatggg gatcaactct tcctcacgaa atccgacgat cttgttctga
     1141 tcgaggaagt tgacggtata gatcagctgg tcctgcaaaa aggtgccgac attcatcacg
     1201 aatccggtcg agccgcgacg gatcagcagc gtgccggttg gcacccccgg ataagtcccg
     1261 tcattgcgca cattgcggat gatgcggact tcctcgccga attcgtattt aggcaacatc
     1321 ttgtttgttc accgtgatcg aactcagcgg cacgaacttg acctgtggtt catgcatgcg
     1381 gaacaccttg aacacctttt cggtcagtgc atccgactgc tcgaaatcct gataggcctg
     1441 gcgcaacagg gtcgtgtaca ccacccggcg cgcggcctcc acctcgggca aggtcgtctt
     1501 gccgagatag tcgtggtaac gctgcaggat atgcaggcga ttcacatgca ccacggaagg
     1561 ctcgaattcg atgccgaagt aattgaggaa atcttcggcg gaggacagtt cttccagtgc
     1621 ctcgtccagt gtcagctcgt ccatctcgtg ctcctttcaa aaatctcttc gttgttaatc
     1681 gactggctgc gcaccacatc cagcagcgct tttccaccca tacgatcatg gctgtccagt
     1741 tccaccagtt tttccagcat cgcccgtccg gtcgggatgt tgcccagcct caggttggta
     1801 tagcccgccg ccttgagcac catcagatag aagcgcacca agcccatgga acgcatcacc
     1861 cctgccccca cattcgcctc ggccagatag acccaggtat cgggaaactc cagcctgcgg
     1921 ccgacgatgg ccagcgcgct ttccgccacc agcagggcat cctccagacg gtgctggtag
     1981 aagtagtagc gatatagcgc gaccagtacg gtgagattct gcggagccat gaagttggct
     2041 cgcagcagca tcagctcggc ctcatcgctg ccgtagagat tcgccgccgt atcgagcagg
     2101 cgacctaccg ccgcactcac cggttcgtcg aagtacagct cggcttgttc aaaatccatc
     2161 aggtccattt cattcttttc tgatcgtgcg cgccacttcc gtgccgcaaa gttcatccgc
     2221 ctcacaggtc cggcattgcc cttcgaccgc agtgccctgc ctgtgcaatt tcgcctccag
     2281 catctcgata cgttccatca gacaagcgat ggcacgtccc accggatcgg ggatcaggtg
     2341 atggtcgaga ttgatgccgt agatgttgtc cgcattggcc tcttcggtgc gcaacacgat
     2401 cttgccgggg atgccgatga cagtacgatg cgagggcaca tccttgacca ccaccgaatt
     2461 cgcaccgacc cgcacctgct cgccgagcgt gatcgcaccg agtatcttcg cgcccgcacc
     2521 gaccacgaca ccgtcaccca gcgtggggtg acgcttgccc ttgttccagg ttgtgccgcc
     2581 gagcgtcacg ccgtgataga gcgtcacgtc gtcgccgatc tcggcggtct cgccgatcac
     2641 cacgccggcc ccgtgatcga tgaagaagcg cctgccgatg gtcgcgcccg gatggatgtc
     2701 gatattggtc agcagtcgtg cgaaccatga caggatgcgt gccgggaaac acaggccaaa
     2761 cttccacaag cgatgcgcga tgcgctgcat gaggatggca tgcacgccgg gataggtggt
     2821 cagcacttcg agtcgcgagc gtgccgccgg atcgcgctgg aacacacagc tcacgtcctc
     2881 acgcagcaac ttaagcattg agggtgtctc cggttttggt gctgcttcga tcaggacgtc
     2941 ttccatgtcg cgctcctaat gcgccatacc gggctgattg cgcccgatat aacgctgata
     3001 aagggacagc agctcctggt ctgccggcga acgcttggcg cgctcggcaa aagcccggat
     3061 atccggcaac agcgcacgcg cttcgacagg actcaggaac aggcccatcg tggcataggc
     3121 ggcgacaatg ccatgcgtac cggaatgttt gcccaacacc agcttgtgac gccgcccgac
     3181 ctccgaggga tcgaagccct gataggtctc cggatccttc agcatgccgt ccacatggat
     3241 gcctgcctca tgggtgaaca ctccctcccc caccagactc ttctgccagt gcaccggacg
     3301 ccccgaggcg gccgccactt tttctgacag ctcagggaag ttgctcagat cgataccggt
     3361 ctggagctga tagagcttgt tcagtccaag tgcgacttct tccagcggcg cattgcccgc
     3421 acgctcgccc agaccgttca ccgtggtgtt gatatgtgtc gcccccgcca cagcagccgc
     3481 cagactgttg gcggtcgcca gccccagatc gtcatgcgca tgcatctcga tctcgatgtc
     3541 caccgcctga cgcaaggctg cgatcgcctt gtgcacgccg aacggctcca tcacgcccag
     3601 cgtatcggcg aagcggatgc gttgcgcacc ggcggcctgt gcggcttcgg ccacctgcag
     3661 catgaattcc ggatcggcgc gcgacgcatc ctcgcagccg acgatgacat cgcgaccgaa
     3721 gtggtaggcg cgcggcacga tgtcgtagat ggtatcgagc acccactgac ggtcgcgtcc
     3781 gagcttcttc ttcaggtgga tgtccgacac cgggatggac aggtcgacca gcttcacatc
     3841 gacctgctcg cagaccacga gatcctcgag acacatgcgg ttccacacga tcagcttcgc
     3901 cttgaggcca agtcccgcga cggcgttgat gctgtcgcgc tcttcctcac ccatcgccgg
     3961 gatgcccact tccagctccg gcacgcccat cgcatcgagc tgacgcgcga tggccagctt
     4021 ctcgtccagc gtgaacgaca ccccggcggt ctgttcgccg tcgcgcaagg tggtgtcgtc
     4081 gatgatgatg gcgcgagaca tgatggctgc cttatgcgta agtcggcgcg aacgccttct
     4141 ccggctcagc ggcaggacca tcgccatccc agtacggcga cagcttgcgc agttgtgcga
     4201 tgatctccgg cacgacctgg atcacacgat ccacttcatc ctcggtgctg taacgcgaca
     4261 gggagaagcg caccgtgcca tgggctgcgg tataagggat gcccatcgca cgcatcacgt
     4321 gcgagggttc cagcgaaccc gaggtacagg ctgaaccgct ggatgccgcg ataccgaact
     4381 tgttgagcag caacagaatc gcctcgccct cgatgaactc gaacgcgatg ttgctggtgt
     4441 tgggcagccg gtcatccggg ttgcccgtca cgaacgagcg cggcacacgg gccaggatgc
     4501 ctgcttcaag tttgtcgcgc aggcgtgcca cctcagtgtt ctcgaaagcc atcgcctcca
     4561 gcgccatctc ggcggccttg cccaacccca cgatggaggt ggtgttctcg gtgccggcac
     4621 gacgaccgcg ttcctgatgg cctccacgca acagcggacg gaaacgcaca ccgcgcttga
     4681 ggtacagcac accgatgccc ttgggggcat gcagcttgtg ac
//
LOCUS       DASUIV010000155         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_872575, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000155 DASUIV010000000
VERSION     DASUIV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872575"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1374
                     /gene="nuoF"
                     /locus_tag="VFW59_05755"
     CDS             109..1374
                     /gene="nuoF"
                     /locus_tag="VFW59_05755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5363753.1"
                     /translation="MRGPVILTTMEFDQPWTLENYLKVGGYQALRRVLTEKMSPDAII
                     AEVKSSALRGRGGAGFPTGLKWSFMPKNYNGDKYIVCKTDEGEPGTCKDWDILLYNPH
                     LLIEGMAIAAYTMGVKTGYNYVHGEIFEAYERMEEACELARAAGYLGDNILGSDFSFD
                     LHNHLGYGAYVCGEETALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETFASIP
                     YIINNGGQKFLELGKPNNGGTKLFSVSGHVNNPGNFEVPMGTPFSELLKMAGGVRTGH
                     KLKAVIPGGSSMPVLPGEVMMDLTMDYDSISKAGSGLGSGAVIVMDDTTCMVRALERL
                     SYFYHEESCGQCTPCREGTGWLYRIVHRIEHGEGRPEDLDLLLSVGENIAGRTICALG
                     EAAVWPVQGMLKNFRSEFEYHIEHKRCLV"
     gene            1389..>3383
                     /gene="nuoG"
                     /locus_tag="VFW59_05760"
     CDS             1389..>3383
                     /gene="nuoG"
                     /locus_tag="VFW59_05760"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX5363754.1"
                     /translation="MINIEIDGKLVETERGSTVMDAARKAGVHIPHFCYHKKLSIAAN
                     CRMCLVEVEKAPKPLPACATPAADGMKVKTHSEMAVKAQKGVMEFLLINHPLDCPICD
                     QGGECMLQDVAMGYGGTASRYTEEKRVVMGKDIGALISTEEMSRCIQCTRCVRFGQEI
                     ADIMELGMAFRSEHAEIMSFVNSSVDSELSGNMIDLCPVGALTSKPFRYTARSWELSR
                     RKSVSPHDGLGSNLAIHVKNNQVMRVTPVENEDINECWLSDKDRFSYEGLNSQDRLTK
                     PMLKQDGKWQEVEWQAALEYVANALRQVSSGAGADQVAALATPHSTLEEMFLLQKLVR
                     GIGSGNVDFRLRQSDFSADGKQAGVPWLGMPVAEINNRDRFLIVGSFLRKDHPLLAQR
                     IRQAVKHGAQANVVHSADDDLLMKVANKAIVAPSSLPDLLAQVLKALAAEKQGNVQGM
                     QDVQVGSEAAAIAKSLASGERAAVLLGNFAQQHPQAAQIAVLAEQIAALCGAKFGYLG
                     EAANSVGGYLAGAIPGANGMNAVQMVAAPRKAYLLLNVESELDMQNPQQAIAAMQSAD
                     LVVAMSAYKHHALDYADVLLPIAPFTETSGTFVNTEGRAQSFKGAVKPQGEARPAWKV
                     LRVLGNLLDVAGFDYDTSEAVRDEVLGGGDIAGKLNNSLQA"
BASE COUNT          717 a          934 c         1085 g          647 t
ORIGIN      
        1 tatgggggcc tgcaaggatg cgccggtgct gacgatcagc aacaaaaaga tctgcgcccg
       61 catgagcaag gaaaagatcg acaaaatttt tgcggagctc gataaatcat gagaggccca
      121 gtgattctta ccacgatgga gttcgaccag ccctggacgc tggagaacta tctcaaggtc
      181 ggcggttatc aggctttgcg ccgcgtgctg accgaaaaga tgtcgccgga tgcgatcatc
      241 gccgaggtca agtcgtctgc gttgcgcggg cgcggcggtg cgggcttccc aaccgggctg
      301 aagtggagtt tcatgcccaa gaactacaac ggcgacaaat atatcgtttg caagaccgac
      361 gagggggagc caggcacctg caaggactgg gacatcctgt tgtacaaccc gcatctgttg
      421 atcgaaggca tggcgatcgc cgcctacacg atgggcgtga agaccggcta caactatgtg
      481 catggcgaga tcttcgaggc ctatgagcgc atggaggaag cctgcgagct cgcgcgcgcc
      541 gccggttatc tgggcgacaa cattctgggc agcgatttca gttttgatct gcacaaccat
      601 ctgggctacg gcgcttacgt ctgcggcgaa gagaccgcct tgctggagtc gatcgaaggc
      661 aagaaaggac agccgcgctt caagccgccg ttccccgcca gcttcggtct gtacggcaag
      721 ccgaccacga tcaacaacac cgagaccttc gccagcattc cgtacatcat caacaacggc
      781 ggacagaagt tcctcgaact gggcaagccg aacaacggcg gcaccaagct gttctcggtc
      841 tccggccatg tgaacaatcc gggtaacttc gaagtgccga tgggtacgcc gttcagcgaa
      901 ttgctgaaaa tggcaggcgg cgttcgtact ggacacaaac tcaaggcggt gatcccgggc
      961 gggtcgtcca tgccggtatt gccgggcgag gtcatgatgg atctgaccat ggactacgac
     1021 tcgatttcca aggcggggtc cggtctgggc agcggtgcgg tgatcgtgat ggatgacacg
     1081 acctgtatgg tgcgtgcgct ggagcgtctg tcctatttct atcacgaaga gtcctgcggg
     1141 cagtgcacgc catgccgtga aggcacgggc tggctgtatc gcatcgtgca tcgcatcgag
     1201 cacggcgagg gcaggccgga ggatctggat ctgttgctga gtgtgggcga gaacatcgcc
     1261 ggccgtacca tctgtgcctt gggcgaggcc gctgtctggc cagtgcaggg catgctcaaa
     1321 aacttccgca gcgaattcga atatcacatc gaacacaagc gttgcttggt gtaagggtga
     1381 ggtgagcaat gatcaatatt gaaattgatg gcaagttggt tgagaccgaa cgcggctcga
     1441 ccgtgatgga cgcggcccgc aaggctggtg tccacatccc gcatttctgc taccacaaga
     1501 aactgtcgat tgcggccaat tgccgcatgt gtctggttga agtggagaag gctcccaagc
     1561 cgttgcccgc ctgtgcgaca ccggctgccg acggcatgaa ggtcaagacc cattccgaga
     1621 tggcggtcaa ggcgcagaag ggcgtgatgg agttcctgct gatcaaccat ccgctggact
     1681 gcccgatctg cgatcagggt ggcgaatgca tgctgcagga tgtggcgatg ggttacggcg
     1741 gcaccgcctc gcgttacacc gaagaaaaac gtgtcgtgat gggcaaggac atcggcgcgc
     1801 tgatctcgac cgaagaaatg agccgctgca tccagtgcac ccgttgcgtt cgtttcgggc
     1861 aggaaatcgc cgacatcatg gaactgggca tggcgttccg cagcgaacat gccgagatca
     1921 tgagtttcgt gaacagctcg gtggactccg aactgtccgg caacatgatc gacctgtgcc
     1981 cggtcggcgc gttgaccagc aagcccttcc gctacaccgc ccgcagctgg gagttgtcgc
     2041 gccgcaagtc ggtcagcccg catgacggcc tgggttccaa cctggcgata cacgtcaaga
     2101 acaaccaggt gatgcgtgtc acgccggttg aaaatgaaga catcaacgaa tgctggcttt
     2161 ccgacaagga tcgcttcagc tacgagggtc tgaattcgca ggatcgcctg accaagccga
     2221 tgctcaagca ggacggcaag tggcaggaag tcgaatggca ggccgcgctg gagtacgtgg
     2281 cgaatgcgct gcgtcaggta tccagtgggg ccggtgccga tcaggttgcc gcactggcga
     2341 caccacacag cacgctggaa gagatgttcc tgttgcagaa actggtgcgc ggcatcggtt
     2401 ccggcaacgt cgatttccgt ctgcgccagt ccgatttcag cgccgacggc aagcaggccg
     2461 gtgtcccatg gctgggtatg ccggtcgccg agatcaataa ccgtgaccgc ttcctgatcg
     2521 tgggcagctt cctgcgcaag gatcatccat tgctggcaca gcgcatccgt caggcggtga
     2581 agcatggcgc gcaggccaat gtggttcatt cggctgacga cgacctgctg atgaaagtgg
     2641 cgaacaaggc catcgtcgca ccgagctcac tgcctgatct gttggcgcag gtgctgaagg
     2701 cgctggccgc cgagaagcag ggcaatgttc agggtatgca ggatgttcag gtcggctcgg
     2761 aagccgccgc catcgccaag agcctggcga gcggcgagcg tgctgcggtg ttgctgggca
     2821 acttcgcgca gcagcatccg caggcggcac agatcgccgt gctcgcagaa cagatcgccg
     2881 cattgtgcgg cgcaaaattc ggttatctcg gcgaggctgc gaacagtgtt ggcggttatc
     2941 tggccggtgc gatacccggt gcgaacggca tgaatgccgt gcagatggtt gctgccccgc
     3001 gcaaggctta tctgttgctc aacgtcgagt ccgagctgga tatgcagaat ccgcagcaag
     3061 cgatcgcagc gatgcagtcc gccgatctgg tcgtggcgat gtccgcttac aagcatcacg
     3121 cgctcgatta cgccgatgtg ctgctgccta tcgcaccgtt caccgagact tccggtactt
     3181 tcgtgaacac cgaaggtcgc gcacagagct tcaaaggtgc agtgaagccg cagggcgaag
     3241 cgcgtccggc ctggaaggtg ttgcgtgtgc tggggaacct gctcgatgtg gccggtttcg
     3301 attacgacac cagcgaagcg gtgcgtgatg aagtgctggg tggcggagat attgcaggaa
     3361 aattgaacaa ttccctgcaa gcc
//
LOCUS       DASUIV010000156        24142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1060035, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000156 DASUIV010000000
VERSION     DASUIV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 24142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24142
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060035"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /gene="dxs"
                     /locus_tag="VFW59_05765"
     CDS             complement(<1..1660)
                     /gene="dxs"
                     /locus_tag="VFW59_05765"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX5363755.1"
                     /translation="MPQLLNAIDSPDDLRKLEREQLPQLAQELREFLVESVSKTGGHL
                     SSNLGTVELTIALHYVYNTPDDRLVWDVGHQTYAHKILTGRREAMSRLRMAGGIAGFP
                     KREESPYDTFGTGHSSTSISAALGMAAAARLEGFARRAVAIIGDGAMTGGMAFEALNN
                     AGAMDANLLVILNDNDMSISRPVGALNNYLAKLMSGRFYAAMRRGSEKMLKGMPPVLE
                     FAKRAEEHVKGMVTPSTLFEEFGFNYIGPIDGHDLDVLVDTLGNIRALEGPQFLHIVT
                     QKGKGYAHAEEDCTLYHGVSKFDPASGITPKPAARQTYTEVFGQWMRDMAAQDERLVG
                     ITPAMCEGSGMVEFAQEFPQRYFDVGIAEQHALTFAAGMACDGYKPVVAIYSTFLQRA
                     YDQLIHDVAIQNLPVVLAIDRGGLVGADGATHAGSFDLSYLRCIPNMTVMTPADENEC
                     RQMLYTAYQLDTPSAVRYPRGCGPGVTTTPAMQALPLGRGELRRSGRKVAILAFGSML
                     APALVAGEQLDATVANMRFVKPLDAELVQQLAREHELLVTVEENA"
     gene            complement(1660..2553)
                     /locus_tag="VFW59_05770"
     CDS             complement(1660..2553)
                     /locus_tag="VFW59_05770"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX5363756.1"
                     /translation="MNVDFQSWVAARQSHFEEVLRGLLPSADVAPTRLHEAMRYAVLD
                     GGKRVRPLLAFAAGELAGADPARLDVAAAAVELIHAYSLVHDDMPCMDDDVLRRGKPT
                     CHVQYDEATALLVGDSLQTLAFQLLAEHRLADDASRQLEMVKQLAVASGSRGMAGGQA
                     IDLASVGKSLSLPELEIMHIHKTGALIRAAILLGAHCGNHLNAEQLEKLDRFGKLIGL
                     AFQVVDDVLDCEADTATLGKTAGKDADNDKPTYVSLLGLPGARDMAQRLHREALETLA
                     EFGDAAQRLRELADFIVLRKF"
     gene            complement(2556..2798)
                     /locus_tag="VFW59_05775"
     CDS             complement(2556..2798)
                     /locus_tag="VFW59_05775"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX5363757.1"
                     /translation="MAGKTQKIPGFEAAMAELEQIVADMEAGKLSLEDSLAAYKRGAE
                     LLAFCRTRLEDAQQQVRVLEDGVLKDFSASGESGAN"
     gene            3054..4841
                     /locus_tag="VFW59_05780"
     CDS             3054..4841
                     /locus_tag="VFW59_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5363758.1"
                     /translation="MEKPKKLGKYEIRRELGRGAMGVVYEAFDPLIERTVAIKTILKS
                     SIDKSEVEEAFNRFRREARAAGRLSHPKIVAIYEYGEDDDMAFIVMELIHGKELKEHF
                     DHEDSFSIDDGIRIVLQILDALDYSHARGVVHRDIKPANILITHSGKIKIADFGIAKV
                     DSSLLTQVGAVMGTPTYMPPEQFMGHEVDRRADLYSTGVILYQFLTGKRPFTGGVISV
                     MHQAVNQEATPPSAINPDVSPQLDEVVKKAMAKRPEDRFQSAAEFMAALKAAAQASAP
                     AKTPTSASDKTIVDLHKAFNSDDTIKLPGRTKPTEASRTNDIESWQSITNSQNPADFN
                     RYLQEFPDGEFAQLARLRLESLEKLSAQARAAAERARLEQEAQVRDALAEKRRRMAEQ
                     QAAAEKAQAQAKIQAEAEIRRQREAAEKAQWAQKIAEIKNEADKARAAEAIKREKEAA
                     EQAQRAQELSASLSQRAHKFVEVVSKREAIVDAERKLKMEHKKNLREEVERKKQAKMK
                     VLAKRDAAGIQAPVAVEEKLQRETELAQWDKELAEMKARADNSERLRKEAEERIAQAH
                     KRTRSLILTVGAVLSILLIGVLIALLAAK"
     gene            complement(4870..5784)
                     /locus_tag="VFW59_05785"
     CDS             complement(4870..5784)
                     /locus_tag="VFW59_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5363759.1"
                     /translation="MNDTTPINTNQEKAVMFADVSGSTRLYEVLGDARAFAAIDACLD
                     ILRRLTEAHSGRVVKTIGDEIMAVFPDVMSAVQAACEMQLVMTARAPIDNTRVAIRVG
                     LHFGPVLESTGDGDVFGDTVNVAARMAGIANAGQIITTSTTVALLPPILRASTRTLNA
                     LSIKGKADDIEVREVIWQESEEMTMMVGNTIPPPAAEPTLKLIHLGETFTVDAAHPAI
                     TIGRDEQADIVIEDRRASRMHAKIERRRDKFVFVDISSNGSFVIINGETEIQLRREEL
                     VLRESGSISLGHPYKKDPTEVIEFFCQG"
     gene            5961..7910
                     /locus_tag="VFW59_05790"
     CDS             5961..7910
                     /locus_tag="VFW59_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363760.1"
                     /translation="MPTLLNLPQHIYIFAALLGALLFSFVIFFLIPSFIVSRHLSRAI
                     RQLGALNGKPDGAMDQIFRKTGTLEHLWQEYADTLHKQSADGQPQASRFRSTIPAGVV
                     FRPDILVDIPLRTDFFKHLPGLFTGIGIIGTFYGLLIGLQSFVVSENPVIVRASLTKL
                     LHGVSEAFLVSAAAITLAMVITFIEKLITTRMNAKVEKLVQLLDGLFESGAGEEYLAR
                     LVKASESSSAQTAGLLKGELKQILTELTEQQIAAAQAGSIALGDRIAASIEDSMKAPL
                     TEMANSLKNVHNNQDSAVQTMLTGVLENFSRQMQDLFGGQITGINSMQQQTIDALQAA
                     VATLERMAADVGSEGQRSTAAMAEQLAESIAAAESRQRIMNDKLGEFVEQMRQTSAQT
                     QNEAQERLNSTLTELSERMGTVIEALSDQVRSATDVSMKQQQNLAAQSQNMVGQFSEQ
                     IFAMVEGVNKAVGEMKAAVMAMRNTTGEAMSKLNNGADTLYLAAKDFAKAGQGVTATL
                     DKSAAVATQLTQAAGSVASASKGLNGMLADYNAARDSMIQMVGSLQLLVEQVRRDASM
                     SGEVLARIEAATAKLVEAQKEADSYLTKVSEVIGVAHESFSEGMVKAVGEANRDFHQA
                     LSDSVKLLREGVQELESTLDAATSL"
     gene            7919..8635
                     /locus_tag="VFW59_05795"
     CDS             7919..8635
                     /locus_tag="VFW59_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX5363761.1"
                     /translation="MISKYLILGNRNKDEGEKPFWISFADLMTALMVMFLLVMSVALL
                     AVTNEVSAVDQKKAQREQEIDKILEKIEKAAEQYPGISIDKNRNVIDFGDRARFDTGS
                     SRLSSEQAQYLSQFVSEVLSISRDQLGQKWIKRIVAEGFADQRGDYLLNLNLSLQRSQ
                     RVLCVLLAPSPAQEHFLNPAEQEQVRDLFLVGGYSFNSAKASLEESRRIELRLEFYQL
                     DEIHPALNDIPRGNFGTCRI"
     gene            8640..10241
                     /locus_tag="VFW59_05800"
     CDS             8640..10241
                     /locus_tag="VFW59_05800"
                     /inference="COORDINATES: protein motif:HMM:NF026949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EH signature domain-containing protein"
                     /protein_id="HEX5363762.1"
                     /translation="MKELQQLQRTLRNLNRVALAKLRPGDSIQMLSERTKLKEWIGGS
                     TQLDETTSRTIEDALRAYRENNYLKGLRQIRLVCYGCTQTLDDGYRLIDNREAFEQML
                     LYAGRYGNRPSALRKLYHGLLGCYFSYDPYAPDVSPAARGNWELLRAFLRKHLELVQT
                     RGFTPDWLAALSQHPNLLGEDPCHPYENLVFQGDWSEFDALRELLAISTSSWLVRQMV
                     MSPIKTVESMDDAAFREHIDSILLLLHSYRLYADAGLAILLDRHVRCTDQKISRSLRD
                     FAVALWGNPWLPENSHQWQCSASAREMLAHWLKRHLLKGFFSLLSNDDKKHSRRFNFW
                     ELYSADLTGMYFALGKDALEVSNMEIYKFRRTAKGLITRLPEGKPDVHACIMQFKNHH
                     VVEFNRDNGMAYLYDLKLGTPHFYLSKGWAEIGALSVTEVSQGVDVSRQSRPLRHQDT
                     PQLSWEGVFARELGASENALKAFCQKYQCTYTDSSDQDGRLWIRPRDLARYGPEVWSI
                     LSGWGYTLSNAENGYFRSVSLNISL"
     gene            complement(10294..10770)
                     /locus_tag="VFW59_05805"
     CDS             complement(10294..10770)
                     /locus_tag="VFW59_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX5363763.1"
                     /translation="MSVNKVILVGRLGKDPETRYMTNGEAVSNATLATSENWKDKSGE
                     KQEKTEWHNLVFYRRLAEIAGEYLKKGSQVYIEGRIQSRKYTTKEGQERYITEIIVNE
                     MQMLGGKSSGGGSFEVVDDQSAPARSSAPARSAAPSRPAPAAAPASFDNFDDDIPF"
     gene            complement(10772..12127)
                     /locus_tag="VFW59_05810"
     CDS             complement(10772..12127)
                     /locus_tag="VFW59_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5363764.1"
                     /translation="MTPVERRASFGLAGIYGLRMLGLFIILPVFAQYAEHLPGGESHL
                     LMGVALGAYGLTQAILQIPAGWLSDRYGRKPVIYISLVLFAIGSFIAASADDIYWVIA
                     GRVVQGAGALNAAVMALTADLTREEVRTKAMATIGMTIGVTFSLSLVLSPLLNGMIGV
                     PGLFALTGVLAVLAMAVVRFVIPDPKITRFHSDTEAGHGHFGEVLRNRELLRLDFGIF
                     SLHAILMSVFMQVPFIMKADGLDVADHWHIYLPVMLVAFALMVPPIIIAEKKAKMKQI
                     FMASIALAASAMILLMFAQHSLWGVAIALLLFFTAFNVLEATLPSMISKIAPLSAKGT
                     AMGVYSSVQFLGAFFGAAAGGALMQFVGGDAVFVFSIALLLLWLMAASGMTPPAAVRT
                     RLYHLPELSDAAAVELRRQLLLLAGVREAMVVAGEGMACLKVDMQGFDEAAVEQLIKG
                     A"
     gene            12391..15210
                     /gene="uvrA"
                     /locus_tag="VFW59_05815"
     CDS             12391..15210
                     /gene="uvrA"
                     /locus_tag="VFW59_05815"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5363765.1"
                     /translation="MEQIRIRGARTHNLKNINLDLPRHKLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAIAIEQKATSHNPRSTVGTVT
                     EIHDYLRLLFARAGDPYCPQHDLVLEAQSVGQMVDHVLELPEDTRIMILSPLVVDRKG
                     EQADLFDELRAQGFMRLRVNGTVYEIDSLPKLEKNKKHTVEIVVDRLKVHPDSKQRLA
                     ESFETALRHAEGRAVAVEMDSGKEHMFSAKFACPICNYSLPELEPRLFSFNNPMGACP
                     KCDGLGNISFFDPKRIVAFPTLSLSSGAIKGWDRRNQFYHQLLSGLAKHYEFDLEQSF
                     ESLPEKVQQVVLYGSGREEIAFNYLNERGKLSLREHPFEGVVNNLERRYKETDSPTVR
                     EELAKFLNSCVCTECHGARLRLEARNVRIADKNLHEISALPLKQTLAFFKTLTLSGHK
                     QAIADKIVLEISSRLTFLNNVGLDYLSLERSAETLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNDRLLDTLKMLRDMGNSVIVVEHDEDAILHADYVVDMGPGAGEHG
                     GLIVAQGTPKEILANEQSLTGDYLSGRRSIPMPKEFLKPDPERMLTITGASGNNLKEV
                     TLNLPVGLLVCVTGVSGSGKSTLINDTLYPVVSQHLYRSTTEPAPYAEIEGLEFFDKV
                     INVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPSARERGYGPGRFSFNVKGGRCESC
                     QGDGVIKVEMHFLPDVYVPCDVCHGHRYNRETLEINYKGRNIHQILEMTVEQAHEFFA
                     PVPVIARKLQTLLDVGLGYITLGQSATTLSGGEAQRVKLSLELSKRDTGRTLYILDEP
                     TTGLHFHDIALLLKVIHKLREQGNTVVVIEHNLDVIKTADWVIDLGPEGGDGGGRIIA
                     EGSPKDIAQNKNSVTGKYLKQVMKR"
     gene            15225..16460
                     /locus_tag="VFW59_05820"
     CDS             15225..16460
                     /locus_tag="VFW59_05820"
                     /inference="COORDINATES: protein motif:HMM:NF039793.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363766.1"
                     /translation="MRHRILVTLTLLAIASPGAIALPQQAPATPSIVLPADEGIRFSC
                     PPERLEAIESGMSAYLAKLHIAPALIIARIDRAQGIAVYTLNTPGEDTNTLDLRDRPE
                     LRIEDDTVSLPAGHGKKKRVITVSKKEILLALLQHGRLTEFKDSACDVQALQDHVGIR
                     QNIAAWGENLNWVWPNGGPAKWNDKYWDRGTPRAGFPLHQALDDVFMNPRRYAIGCYT
                     SSKLLMVQGMLDYFRRIKQDPARQKQLEHRLMKDGEPLKNIEPGRMWDFEADFDPQEL
                     NRPGKLLRIAYGIAPKNFIPGDWIHFFNSDPVSYQKTGYEGSNPVYLGRNKFADYFND
                     HDHSYSYQQKLDEVYQWRNGVFSRSRDAAKIKPLSAGEIERLSLPPARGGLLTDMRVS
                     PYFFGHERLPELDTSPAAE"
     gene            complement(16553..16628)
                     /locus_tag="VFW59_05825"
     tRNA            complement(16553..16628)
                     /locus_tag="VFW59_05825"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16593..16595),aa:Thr,seq:tgt)
     gene            complement(16664..16739)
                     /locus_tag="VFW59_05830"
     tRNA            complement(16664..16739)
                     /locus_tag="VFW59_05830"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16704..16706),aa:Lys,seq:ttt)
     gene            complement(16823..17509)
                     /gene="queC"
                     /locus_tag="VFW59_05835"
     CDS             complement(16823..17509)
                     /gene="queC"
                     /locus_tag="VFW59_05835"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX5363767.1"
                     /translation="MTTKNAVVLLSGGLDSATVLAMARAQGYTCYALSVDYGQRHHSE
                     LAAAQRVASMLGAHEHRVVYIDLTGFGGSSLTDNNIAVPQQLAAGIPSTYVPARNTIM
                     LSLALAWAEVLQAQDIFIGVNAVDYSGYPDCRPAYIEAFERMANLATKAAVEGHALTL
                     HAPLLNLSKAEIIRQGTALGVDYAQTVSCYQADEAGRACGVCDSCRLRRAGFSAANID
                     DPTVYLAQEI"
     gene            complement(17506..18165)
                     /gene="queE"
                     /locus_tag="VFW59_05840"
     CDS             complement(17506..18165)
                     /gene="queE"
                     /locus_tag="VFW59_05840"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX5363768.1"
                     /translation="MSHVTQDAQLRISEIFFSLQGETRRIGLPTVFIRLTGCPLRCTY
                     CDTSYAFTGGRNASLTEILEQVASYAPRYVCVTGGEPLAQKNALPLMTALCDAGYEVS
                     LETSGALDIGGVDARVMRVLDIKTPASGEAGKNRWDNLALLNAHDEIKFVLCDENDYL
                     WAKQTLAQHRLAERCEVTFSPAHASLSATQLAEWILRDRLPVRMQVQLHKILWPNEAG
                     R"
     gene            complement(18171..18908)
                     /gene="ybgF"
                     /locus_tag="VFW59_05845"
     CDS             complement(18171..18908)
                     /gene="ybgF"
                     /locus_tag="VFW59_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX5363769.1"
                     /translation="MLRQRALLLLSLFFVAPAQAGLFSDDEAHKQIQLLEARLSKVEA
                     ASEQQTKSMLDLLTQIETLNGEMRKLRGQNEELVRGLQDAEKREKDFYVDLDTRVRHF
                     ESSDTVGQGDAAKSDSGDTAQENRAYEAAYALFKAGNHLDAIKAFQEFTRKYPESVYV
                     PNASYWLGNAQYAMKNYKAALETYRVLRKDYPSQPKDAEVLFNIAGCQQELKQGENAQ
                     KTLKLLISKYPDSDVAAQAKKQLASKK"
     gene            complement(18912..19415)
                     /gene="pal"
                     /locus_tag="VFW59_05850"
     CDS             complement(18912..19415)
                     /gene="pal"
                     /locus_tag="VFW59_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX5363770.1"
                     /translation="MKKLVISLVLVNLLAACASNKPAETTAAPAGSAAGTAASTQAAS
                     ASSVDALNDPASILAKRSAYYPFDVSVVQEADKPMIQAHGQYLANHADRKVRLEGNCD
                     ERGSNEYNLALGQRRADGVKKMLEVSGAKTAQLDSVSYGEEKPKASGHDEASWAQNRR
                     TDLNYAK"
     gene            complement(19439..20704)
                     /gene="tolB"
                     /locus_tag="VFW59_05855"
     CDS             complement(19439..20704)
                     /gene="tolB"
                     /locus_tag="VFW59_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX5363771.1"
                     /translation="MQVIRGMVALLLLVQLSVASAALNIEIIGAGEKQIPVALVPFGG
                     DSELSKTINEVVTGDLQRSGLFRMVDLAGKFLDEPSQVSYADWQVRGADALSIGRVTA
                     QPNGRIEAHFRLFDVVKQNELIAQAVSAGDGQARAVGHRIADMIYEKLTGDKGVFSTR
                     IAYVNRQGSNFRLIVADSDGYNEQVILAHNEPIMSPSWSPDGSHLAYVSFETGHAVVY
                     VQSLLTSQRKVLADFRGSNSAPAWSPDGKQMAVVLTRDGGSQIYLVRPDGSNLRRITF
                     SGVIDTEPNFSPDGQYLLFSSDRGGSVQIYRMPVNGGPEQRMTFGEGNNFSPRYSPDG
                     SSFVFAHRSSGKFYIAVQDFQTGQMEILTEGGWEKKPSFAPNGKLILFASEARGRGIL
                     ATVSSDGRVKQHMFTQGGDAREPVWGPNP"
     gene            complement(20710..21381)
                     /gene="tolA"
                     /locus_tag="VFW59_05860"
     CDS             complement(20710..21381)
                     /gene="tolA"
                     /locus_tag="VFW59_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX5363772.1"
                     /translation="MSPVVYHEANRLPAGILALAVHGAFFALLYFGFSWQTEPVAAMS
                     VELWDSIPSAPSAKPKVEEKVEQKVEAPAPPPAPEKQPEIALPDKKAKPVEKKPEKKP
                     EKKVKEPQVDEQALREQAEQTERAAATGRMVDEYTGKISAKIRSRIVMPPGVPDDARA
                     EFAVTVLPGGSVLNPRLLKSSGNAAYDNAVERAILKAQPLPLPPDAALFNKFREMKLG
                     FRPKE"
     gene            complement(21378..21800)
                     /gene="tolR"
                     /locus_tag="VFW59_05865"
     CDS             complement(21378..21800)
                     /gene="tolR"
                     /locus_tag="VFW59_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX5363773.1"
                     /translation="MRTNRRSNNRLMNEINVVPYIDVMLVLLVIFMVTAPMMNPGQID
                     LPQVGQSLAPPVAPLEVIIKKDSSLALRDHSRGSKESIVTRDELIAILKGRQGDLADQ
                     AVVISADKNVRYEEVINVMDLLQQQQIKKVGLLTQNRK"
     gene            complement(21826..22509)
                     /gene="tolQ"
                     /locus_tag="VFW59_05870"
     CDS             complement(21826..22509)
                     /gene="tolQ"
                     /locus_tag="VFW59_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX5363774.1"
                     /translation="MEVTQDLSFVHLISNASVLVQLVMGLLLLVSMMSWWYIFLKMFS
                     IRAEVRLTEAFEDEFWRNHDLNELYRRSSGSSLHGEQGALERIFAAGFAEFVKLKKQQ
                     HGGDVSAVMDGTRRAMRATYQREMDRLESHLAFLASVGSVSPYVGLFGTVWGIMNAFR
                     GLANVGQATLAHVAPGIAEALVATAMGLFAAIPAVIAYNRYSHDINRLSTRFESFTEE
                     FSNVLQRQP"
     gene            complement(22650..23057)
                     /gene="ybgC"
                     /locus_tag="VFW59_05875"
     CDS             complement(22650..23057)
                     /gene="ybgC"
                     /locus_tag="VFW59_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX5363775.1"
                     /translation="MSKHKIFSWPTRVYYQDTDAGGVVYHANYVNFMERARTEWLRTF
                     GYSNAGLMQELGVMFVVRKLELNYLRPALLDDMLDVTAQLKEVGRSRITLEQTVTRGD
                     ELMTVAEVHLVCVGVESFKPVSVPEVLSKHWKI"
     gene            complement(23050..24105)
                     /gene="ruvB"
                     /locus_tag="VFW59_05880"
     CDS             complement(23050..24105)
                     /gene="ruvB"
                     /locus_tag="VFW59_05880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5363776.1"
                     /translation="MIHNDDLSAASRIISAAPASRQEEALERALRPKQLDEYVGQEKI
                     RGQLEIFIEAAKRRGEPLDHVLLFGPPGLGKTTLAQIIAREMGVNIRHTSGPVLERAG
                     DLAALLTNLEPNDVLFIDEIHRLSPVVEEILYPALEDYQIDILIGDGPAARSVTLDLP
                     PFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPEELQRIVMRSSGLLEMNLSQDGALE
                     IAKRSRGTPRIANRLLRRVRDFADVKADGDASREVADAALTMLDVDTRGLDLMDRKLL
                     LTVIEKFMGGPVGVDNLAAAIGEERDTIEDVLEPYLIQQGYLQRTPRGRMATANAYKH
                     FGLAVASNPVMGDLLDE"
BASE COUNT         5108 a         7686 c         6863 g         4485 t
ORIGIN      
        1 cggcattttc ctccaccgtc accaagagct cgtgctcgcg tgccagctgc tggaccagtt
       61 cggcatccag cggtttgaca aaacgcatgt tggccaccgt cgcatccagt tgttcgcccg
      121 ccaccaacgc gggggccagc atcgaaccga acgccagtat cgccaccttg cgaccactgc
      181 gacgcaactc gccacggccc agcggcaagg cctgcattgc cggcgtagtc gtcacccccg
      241 gcccgcagcc tcgcggatag cgtaccgcgc tcggcgtatc caattgatac gcggtgtaca
      301 acatctgacg acattcgttt tcatcggcag gcgtcatcac cgtcatgtta gggatgcagc
      361 gcagatagga cagatcgaag ctgcccgcat gggtcgcacc gtccgcgccg accagaccgc
      421 cgcgatcgat ggccagcacg accggcaggt tctggatcgc gacgtcgtgg atcagttgat
      481 cgtaggcccg ttgcaaaaag gtcgagtaga ttgccaccac cggcttgtag ccgtcgcatg
      541 ccatgcccgc cgcaaaggta agtgcatgct gctcggcgat gccgacatcg aaatagcgct
      601 gcgggaattc ctgcgcgaat tcgaccatgc cggaaccttc gcacatcgcc ggtgtgatgc
      661 cgaccaggcg ctcgtcctga gccgccatgt cacgcatcca ctggccgaac acctcggtgt
      721 aagtctgcct ggcagccggt ttcggggtga tgccgctggc cgggtcgaac ttggagacgc
      781 cgtgatacag cgtgcagtcc tcctcggcgt gcgcgtaacc cttgcccttc tgcgtgacga
      841 tatgcaggaa ctgcggccct tcgagggcac gtatgttgcc cagcgtatcg accaacacgt
      901 cgagatcgtg gccgtcgatc gggccgatgt aattgaaacc gaattcctcg aacaaagtgc
      961 tcggcgtgac catgcccttg acgtgctcct cggcgcgctt ggcgaactcc agcaccggcg
     1021 gcatgccctt gagcatcttc tcgctgccgc gccgcatcgc tgcgtagaaa cgccccgaca
     1081 tcagcttcgc caaatagttg ttcagcgcgc cgaccggacg cgaaatggac atatcgttgt
     1141 cgttgaggat caccagcaga ttcgcatcca tcgcgccggc attgttcagc gcttcgaatg
     1201 ccatgccacc ggtcatcgca ccgtcgccga tgatcgccac cgctcgccgc gcgaaccctt
     1261 ccagccgcgc cgccgcagcc atgcccagcg ccgccgaaat cgaggtgctg gagtgtccgg
     1321 taccgaaggt atcgtaggga ctctcctcgc gcttgggaaa accggcgata ccgcccgcca
     1381 tgcgcaagcg gctcatcgcc tcgcgccgcc cggtaagtat cttgtgcgca taggtctgat
     1441 gaccgacatc ccacaccagc cggtcatccg gcgtgttgta gacgtaatgc agcgcgatgg
     1501 tcagctcgac cgtgcccaga ttagaggaca gatgcccgcc ggtcttgctc accgattcga
     1561 ccagaaactc gcgcaactcc tgcgccagct gcggcaactg ttcccgttcc agcttgcgca
     1621 gatcgtccgg ggagtcgatg gcgttcagca attggggcat cagaactttc tcaatacgat
     1681 gaaatcggcc agctcgcgca aacgttgcgc cgcatcaccg aattcagcca gggtttccag
     1741 cgcctcgcga tgcaggcgtt gcgccatgtc gcgcgcaccc ggcaatccga gcaggctgac
     1801 ataggtcggc ttgtcgttat ccgcatcctt gcccgccgtc ttgcccagcg tcgcggtatc
     1861 cgcctcgcaa tcgagcacat cgtccaccac ctgaaatgcc aggccgatca gcttgccgaa
     1921 gcggtccagc ttttccagct gctccgcatt caaatggttc ccgcaatgcg cgcccagcag
     1981 gatcgccgca cggatcagcg cgccggtctt gtggatatgc atgatttcca gctcgggcaa
     2041 cgagagcgac ttgcccacgc tggccagatc gatcgcctga ccgcccgcca tgccgcgcga
     2101 gccggaagcc acggcgagct gcttgaccat ctccagttgg cgcgacgcat cgtcggcgag
     2161 gcgatgttcc gccagcagct ggaacgccag cgtctgcagg ctgtcgccga ccagcagcgc
     2221 ggtcgcctcg tcgtattgca catggcaggt cggcttgccg cgacgcagca cgtcgtcgtc
     2281 catgcagggc atgtcgtcat gcaccagcga ataggcatgg atcaattcga ccgcagcggc
     2341 agcgacatcc agccgcgcgg gatcggctcc ggccagctcg cctgctgcga acgccagcag
     2401 cgggcgcacg cgcttgccgc cgtccagcac cgcgtaacgc atcgcctcat gcagacgagt
     2461 tggcgcgaca tcggcgctag gtaacagccc gcgcagcacc tcttcgaaat gggattgccg
     2521 ggccgcaacc caactttgga agtcaacgtt catgctcagt ttgccccgct ctcgccactg
     2581 gcggagaaat ctttcagtac accgtcttcc agcacgcgca cctgttgctg cgcatcttcc
     2641 agccgggtgc ggcagaacgc cagcaactcg gcgccgcgct tgtaggcggc cagcgaatct
     2701 tccagcgaca acttgcccgc ctccatatcg gcgacgatct gctccagctc ggccatcgcc
     2761 gcttcgaaac ccggtatttt ctgcgtttta cccgccatga ttgtctctgc tagcgaaaga
     2821 gaggcatttt acccaagggg gctgccctga gccaattttt atgcatggcc atgcaacatg
     2881 ccgcagttaa atgtaaaaac gtcgttcttg cgaaccaagc cccgaaaaat ccgcaccgag
     2941 ggaaagacat caacatggaa aaccatccaa gtcatggcag aatgctgaac taatagactt
     3001 gcgcgagaac taaggaagct tgggctcgtt aagcattaca agtacaaaaa gacatggaaa
     3061 aacctaaaaa gctgggcaag tacgaaatcc ggcgggagtt gggccggggt gcgatggggg
     3121 tcgtctacga ggcgtttgat ccgcttatcg aacgcacggt cgccatcaaa acgatcctga
     3181 aatcctccat cgacaaatcg gaagtcgaag aggccttcaa tcgctttcgt cgtgaggctc
     3241 gcgcagccgg tcgcctgtca caccccaaga tcgttgccat ctacgagtac ggcgaagacg
     3301 atgacatggc cttcatcgtg atggagctta tccacggcaa ggaactgaag gaacacttcg
     3361 atcacgagga cagcttcagc atcgatgacg gcatacgcat cgtgctgcaa atcctcgatg
     3421 cgctcgatta ttcgcacgcc cgtggcgtgg tacaccggga catcaaaccg gccaacattc
     3481 tgatcaccca tagcggaaaa ataaaaatcg cggatttcgg catcgcaaaa gtcgattcgt
     3541 cgttgctcac ccaggtcggc gcggtcatgg gaactccaac ctatatgcct ccggaacagt
     3601 tcatggggca cgaagtggat cggcgcgccg acctctactc taccggcgtc attctgtatc
     3661 agttcctgac cggcaaacgc ccattcaccg gcggcgtcat ctcggtcatg caccaggcag
     3721 tcaatcagga agccactccg ccctccgcga tcaatccaga tgtttcaccg caactggatg
     3781 aggtggtaaa aaaagcgatg gccaaacggc cggaagatcg ttttcaaagc gcagcggaat
     3841 tcatggccgc gctcaaggcg gccgctcaag catctgcccc ggccaaaacg ccgacaagcg
     3901 cctccgacaa gaccattgtt gacctgcaca aagcattcaa ctccgacgac accatcaagc
     3961 tgccgggccg gaccaagccg acagaagcca gccggacgaa cgatatcgag tcctggcaaa
     4021 gcatcacaaa cagccagaat ccggccgatt tcaaccgcta cctgcaagaa ttccccgatg
     4081 gggagtttgc gcaattagcg cgattacgtc tcgagtcgct ggagaaacta tcggctcagg
     4141 ctcgcgcagc cgcagagcgg gcacgcctcg aacaagaagc ccaggtgcgt gatgcacttg
     4201 cagagaaaag aaggcggatg gccgaacagc aggctgcagc cgaaaaagcc caggcacaag
     4261 ccaagattca ggccgaagcc gaaatcagac gccagcgaga agccgcagaa aaagcgcagt
     4321 gggcgcaaaa gatcgcagaa atcaaaaacg aagcggataa agccagagcc gccgaagcca
     4381 tcaagcgcga aaaagaagcg gcggagcagg cgcagcgcgc ccaggagtta tcggcgtctt
     4441 tatcacaacg cgcccacaag ttcgtcgaag tcgtctccaa acgagaggcg atcgtcgatg
     4501 ccgaacgcaa gctgaagatg gagcacaaga agaatctgcg tgaagaagtg gaacgcaaaa
     4561 aacaggccaa gatgaaggtg ctcgccaaaa gggatgccgc aggaattcag gcccctgttg
     4621 cagtcgaaga aaagctgcaa cgcgaaaccg aactcgccca atgggataaa gagctggccg
     4681 agatgaaagc gcgtgccgac aactcggaac gcctcaggaa agaagcggaa gaacgcatcg
     4741 cacaagcgca taagcgcacc cgctccctga tcctcaccgt cggcgcagta ttatcgatac
     4801 tgctgatcgg cgttttgatc gctctgctcg ctgcaaaata actgcccgcc atcagaccgg
     4861 gggcgccgtt cagccttggc agaagaactc gatgacctcg gtgggatcct tcttgtaggg
     4921 gtgcccaagg ctgatactgc cactctcgcg caacaccaat tcctcacggc gcaactggat
     4981 ttcagtttca ccgttaatga tcacgaagct tccatttgag ctgatatcga cgaacacgaa
     5041 cttgtcgcgg cgacgctcga ttttggcatg catccgcgac gcccggcgat cctcaatcac
     5101 gatatccgcc tgctcatcgc ggcctatcgt gatggcagga tgagcggcat cgaccgtgaa
     5161 agtttcaccc agatggatca gcttcagggt cggctcggca gcaggaggcg gaatggtgtt
     5221 gccgaccatc atggtcattt cttcgctttc ctgccagatg acctctctga cctcgatatc
     5281 gtcagccttc cctttgatcg acagcgcatt caaggtgcgc gtactggcac gcaagatggg
     5341 cggaagcagc gccaccgttg tcgaggtggt gatgatttga ccggcattgg caatgcctgc
     5401 catgcgcgcc gccacattga ccgtgtcgcc gaacacatcg ccatcgccgg tactttccag
     5461 caccgggccg aaatgcaaac cgacgcgaat cgccacgcgc gtattgtcga tcggcgcccg
     5521 cgcggtcatg accagctgca tctcacaggc agcctgcacc gcagacatca catccgggaa
     5581 tacggccatg atctcatcgc cgatggtttt aaccacccgg ccggaatgtg cctcagtcaa
     5641 tcgccgcaat atatcgaggc aggcgtcgat ggcggcaaat gcgcgcgcat cacccagaac
     5701 ctcgtacaga cgggtgctgc cgctcacatc ggcgaacatc acggcctttt cctgattggt
     5761 gttgattgga gttgtatcgt tcacgctatt cattccaatt gaatccagac gcgcattata
     5821 agcacaggat caacctccgc cgagcagcaa cccggcacgg gtaaaattgc ccacccccga
     5881 ggcggggact tgcgtcataa ttccaaaaga atttcccggt tcgcgggcca ttattcaata
     5941 accgcacgat ttttagcaaa atgcccacac tcctcaatct gccccagcac atctacattt
     6001 tcgccgccct gctcggcgcg ttactgttct cgtttgtgat cttcttcctg atcccgtcat
     6061 ttatcgtatc gcgacatcta tccagagcga tcaggcaact cggcgcactg aacggcaagc
     6121 cggacggcgc gatggatcag atcttccgca agaccggcac gctcgaacat ctgtggcagg
     6181 aatatgcgga tacgctgcac aagcaaagcg cggatggaca accccaggca tcgcgcttcc
     6241 gctccaccat ccccgccggc gtggtattca ggccggacat cctggtggac atcccgttgc
     6301 gcaccgattt tttcaaacac ctgccaggat tatttaccgg catcggcatc atcggcacgt
     6361 tctatggcct gttgatcgga ctgcagtcct tcgtggtttc cgagaacccg gtcatcgttc
     6421 gcgccagctt gaccaagctg ctacatggcg tatccgaagc cttcctcgtg tcggccgctg
     6481 ccatcacgct ggccatggtg atcactttca tcgaaaagct gatcaccacc cgcatgaatg
     6541 cgaaggtcga aaagctggta cagcttctgg atggactgtt cgagagcggc gcgggcgaag
     6601 agtatctggc gcgactggtg aaagcatcgg aatcgtcctc cgcccaaacc gccggactgc
     6661 tcaagggcga gctgaaacag atcctcaccg agttgaccga acaacaaatt gccgccgccc
     6721 aggccgggtc gatcgccctc ggagaccgca ttgccgccag catcgaagac agcatgaaag
     6781 ccccgctgac ggagatggca aactcgctga agaacgtgca caacaatcag gactcggcgg
     6841 tacaaaccat gctgacgggc gtgctggaga atttcagccg ccagatgcag gacctgttcg
     6901 gcgggcagat caccggcatc aacagcatgc agcagcagac catcgatgcg ctgcaggccg
     6961 ccgtcgcgac gctggaacgc atggctgccg atgtcggatc ggaaggccag cgcagcaccg
     7021 ccgcgatggc cgaacaactg gccgaatcca tcgccgcagc ggagtccaga cagcgcatta
     7081 tgaacgacaa gctcggcgaa ttcgtcgagc agatgcgcca gacatccgct caaacccaga
     7141 acgaagcaca ggaaaggctg aacagcaccc tgaccgagct gtccgaacgc atgggtacgg
     7201 tgatcgaggc gctgagcgat caggtgcgtt cggcgaccga tgtcagcatg aagcagcagc
     7261 agaatctggc ggcgcagagc cagaacatgg tcggtcagtt cagcgagcag atcttcgcca
     7321 tggtcgaggg cgtaaacaag gccgtcggcg aaatgaaagc agccgtcatg gcgatgcgca
     7381 atacgaccgg cgaggccatg tccaaactca acaatggcgc ggacacgctg tatctggcgg
     7441 ccaaggattt cgccaaagcc ggtcagggcg tcaccgccac gctggacaaa tcggccgccg
     7501 tcgccaccca gctcactcag gctgcgggct cggtcgcctc cgcctccaag ggcctgaacg
     7561 gcatgctggc cgactacaac gcggcgcgcg actccatgat ccagatggtc ggctcgctgc
     7621 aactgctggt cgaacaggtg cgccgcgacg cctccatgtc gggcgaggtg ctcgcaagga
     7681 tcgaggccgc cacggccaag ctggtcgagg cacagaaaga agccgacagc tacctgacca
     7741 aggtgtccga agtgatcggc gtcgcgcacg aatccttcag cgaaggcatg gtcaaggcgg
     7801 tgggcgaagc caatcgcgac ttccaccagg ccctgtcgga ttccgtgaag ctgctgcgcg
     7861 aaggcgttca ggaactcgaa agcacccttg atgcggccac cagtctgtaa ggcccgccat
     7921 gattagcaaa tatctgatcc tcggcaaccg caacaaggac gaaggtgaga agcctttctg
     7981 gatctcgttc gccgacctga tgacggcatt gatggtgatg ttcctgctgg tgatgagcgt
     8041 cgcgctgctg gcagtcacca atgaagtctc ggcggtcgac cagaagaagg cgcaacgcga
     8101 acaggagatc gacaaaatcc tggaaaaaat cgagaaggcc gccgagcagt atccgggcat
     8161 ctccatcgac aagaaccgca acgtcatcga cttcggcgac cgcgcccgct tcgacaccgg
     8221 cagctcgcgc ctttccagcg aacaggccca gtatctgagc cagttcgtct ccgaggtgct
     8281 gtccatctcg cgcgaccagc tcggccagaa atggatcaag cgcatcgtcg ccgaaggatt
     8341 cgccgatcag cgcggcgact acctgttgaa cctgaacctc agcctgcaac gcagccagcg
     8401 cgtgttatgc gtgttgctcg cgccttcccc cgctcaggag cacttcctga atccggcaga
     8461 acaggaacag gtgcgcgatc tgttcctggt gggcggctac tcgttcaatt cggccaaggc
     8521 ttcgctggaa gaaagccgcc gcatcgaact gcgcctggag ttttaccagc tggacgagat
     8581 ccaccctgcc ctgaacgaca tccctcgcgg taatttcggc acctgccgga tatgaacgaa
     8641 tgaaagagct gcagcaactg caaagaaccc tgcgcaacct caaccgggtt gcgctcgcca
     8701 aattgcgccc gggcgattcg atacagatgc tcagtgagcg caccaagctg aaagaatgga
     8761 tcggcggctc gacccaactg gatgagacca cctcccgcac catagaagac gcgctgcgcg
     8821 cctatcggga gaacaactac ctcaagggtc tgcgccagat acggctggtt tgttacggct
     8881 gcacgcaaac gctcgacgac ggctaccgcc tgatcgacaa ccgtgaagca ttcgagcaga
     8941 tgctgctcta tgcgggaaga tacggcaatc gcccgagcgc gctgcgcaag ctttatcacg
     9001 gcttgctggg ctgctacttc tcctatgatc cctacgcgcc ggatgtcagc ccggccgctc
     9061 gcggcaactg ggaactgctg agagcgtttt taaggaaaca tctcgagctg gtccagacgc
     9121 gcggatttac gccggactgg cttgcggcac tgtcccagca tccgaatctg ctgggcgaag
     9181 atccctgcca cccctacgag aatctggtgt ttcagggcga ctggtcggaa ttcgacgcac
     9241 tgcgcgagct gctcgcaatc agcaccagct cgtggctggt acggcaaatg gtcatgtccc
     9301 cgatcaagac cgtggaaagc atggacgatg cagcattcag ggagcacatc gacagcatcc
     9361 tgctgctgct gcacagctat cggctctacg ccgacgcggg actggcgatc ctgctggacc
     9421 gccatgtgcg atgcaccgac cagaagatca gccgctcgct cagggacttc gccgtcgcgc
     9481 tgtggggcaa cccctggcta cccgagaata gccaccagtg gcaatgcagc gcgagcgccc
     9541 gggagatgct ggcgcactgg ctcaagcgcc atctgctgaa aggcttcttc tcgctgctgt
     9601 ccaacgacga caagaagcat tcacggcgct ttaacttctg ggaactctac agcgcagact
     9661 tgaccggcat gtacttcgca ctggggaaag acgcgctgga agtcagcaac atggaaatct
     9721 acaaattccg ccgcaccgca aaagggctga tcacccggct ccccgaaggc aaacccgacg
     9781 tacacgcctg catcatgcaa ttcaagaacc accacgtggt ggaattcaat cgtgacaacg
     9841 gcatggccta cctctatgac ctgaagctgg gaacccctca cttctacctg agcaagggct
     9901 gggccgaaat cggtgcgctc agcgtgaccg aagtcagcca gggcgtcgac gtctcgcgcc
     9961 agtccagacc gctacgccat caggacaccc cgcaactgag ctgggagggc gtcttcgcca
    10021 gagagctggg cgcgagcgaa aacgccctca aggcgttctg ccagaaatac cagtgcacct
    10081 ataccgatag cagcgatcag gacgggcggc tgtggatacg tccccgcgac ctcgcccgct
    10141 acggcccgga agtctggtcg atattgagcg gctggggcta caccctgtcg aatgcagaaa
    10201 acggttattt ccgttcggtc agtctgaaca tctcgctgtg acaacaagag gccacccgaa
    10261 ggtggcctct tgcaacgctc aatgacgcat cattcagaag ggaatatcgt cgtcgaagtt
    10321 gtcgaagctg gccggtgcgg ctgccggagc cggacgggac ggtgcagcag aacgggcagg
    10381 cgcgctggaa cgtgccggtg cggactggtc atcgaccacc tcgaagctgc cgccgccgga
    10441 tgacttgccg cccagcatct gcatctcgtt gacgatgatc tcggtgatgt agcgctcctg
    10501 accttccttg gtcgtgtact tgcgcgactg gatgcggcct tcgatgtaga cctgcgaacc
    10561 cttcttcaga tactcgccgg cgatctcggc cagacggcgg tagaacacca gattgtgcca
    10621 ttcggtcttt tcctgcttct cgccgctctt gtccttccag ttttcggaag tcgccagcgt
    10681 ggcattgctc accgcctcgc cgttggtcat gtaacgcgtc tccgggtctt tgcccagacg
    10741 acccaccaga atcaccttgt tcaccgacat atcaagctcc ctttatcaat tgttctaccg
    10801 ccgcttcatc gaagccctgc atatccacct tgaggcaggc catgccttcg ccagccacca
    10861 ccatcgcctc gcgcacaccg gcgagcagca acagttgccg gcgcaattct accgcagccg
    10921 catcgctcag ttcgggcaaa tgatacagcc gggtgcgcac cgccgccgga ggcgtcatgc
    10981 cggaggccgc catcagccag agcaacagca gcgcgatgct gaacacgaac accgcatcgc
    11041 cgccgacgaa ctgcatcagc gcgccgcccg ccgccgcgcc gaagaacgca ccgaggaact
    11101 gcacgctgct gtagaccccc atcgccgtgc ccttggccga caagggcgcg atcttgctga
    11161 tcatcgaggg cagcgtcgcc tccagcacgt tgaacgcggt gaagaacagc agcagcgcaa
    11221 tcgccacgcc ccacaggctg tgctgcgcga acatcagcag gatcatcgcc gacgcagcca
    11281 gcgcgatgga cgccatgaag atctgtttca tcttggcctt cttctcggcg atgatgatgg
    11341 gcggcaccat cagcgcgaac gccaccagca tcaccggcag atagatgtgc cagtgatccg
    11401 ccacatcgag tccgtccgcc ttcatgatga acggcacctg catgaacacc gacatcagga
    11461 tcgcgtgcag cgagaagatg ccgaagtcca gacgcagcag ctccctgttg cgcagcacct
    11521 cgccgaagtg gccgtgaccg gcctcggtat cgctatgaaa acgggtgatc ttcggatcgg
    11581 gtatgacgaa gcgcaccacc gccatcgcca gcaccgccag cacgccggtc agcgcgaaca
    11641 ggccgggcac gccgatcatg ccgttgagca gcggcgacaa caccagcgac agcgagaacg
    11701 tcacgccgat ggtcatgccg atggtcgcca tcgccttggt gcggacttcc tcgcgcgtca
    11761 ggtcggcggt caacgccatc accgccgcat tcagcgcacc ggcgccctgc accacgcgac
    11821 cggcgatcac ccagtagatg tcgtcggcag acgcggcgat aaagctgcca atggcgaaca
    11881 acaccaggct gatatagatc accggcttgc gcccgtagcg gtctgacaac cagccggcgg
    11941 gaatctgcag aatcgcctgc gtcaacccgt aagcgccgag cgcgacaccc atcagcagat
    12001 ggctctcgcc gccgggcaga tgttccgcat actgcgcgaa caccggcagg ataatgaaca
    12061 ggcccagcat gcgcaggcca taaatacccg ccaggccaaa gctggcacgg cgttcgacgg
    12121 gggtcatgga agtgggttcg gacataggga aacgacgatt tttaagagct gcgcatctta
    12181 acagagatga gggggatagt ccgttggcga gctatcggcc gcttggagcg ccaacgctgc
    12241 acctcggcat ccgaaacgaa tcatgcagcc tcccggcctc tgcggataaa cctcccatcc
    12301 agtttccttt ccaaccctca gtggcgacag gttgggaatg catcaaagtt cgcgtatatt
    12361 gtcgggtcgt tttccagaaa gtcgccccgc atggaacaga tccgcatccg tggtgctcgc
    12421 acccacaatc tcaagaacat caatctcgac ctgccgcgcc acaagctggt ggtgatcacc
    12481 ggcctgtccg gctccggcaa gtcctcgctc gcgttcgata cgctgtatgc cgagggacaa
    12541 cggcgctatg tggaatcgct gtcggcctat gcgcgccagt tcctgcagct gatggagaaa
    12601 cccgacgtcg acctgatcga ggggctgtcg cccgccatcg cgatcgaaca gaaagccacg
    12661 tcgcacaatc cgcgctcgac cgtgggcacg gtcaccgaga tacacgatta cctgcgcctg
    12721 ctgtttgcgc gcgcgggcga cccctattgc ccgcagcacg atctggtgct ggaggcgcag
    12781 tcggtaggcc agatggtgga tcacgtgctg gaactgccgg aagacacccg catcatgatc
    12841 ctgtcgccgc tggtggtgga tcgcaagggc gagcaggcgg atctgttcga cgagttgcgc
    12901 gcgcagggct tcatgcggct gcgcgtgaac ggcacggtgt atgagatcga ctcgctgccg
    12961 aagctggaga agaacaagaa gcacaccgtc gagatcgtgg tggacaggct caaggtgcat
    13021 cccgattcca agcagcggct ggccgaatcg ttcgagaccg cgctgcgtca cgccgagggg
    13081 cgcgcggtgg cggtggagat ggattccgga aaagagcata tgttctcggc caagttcgcc
    13141 tgtccgatct gcaactactc gctgccggaa ctcgagccgc gcctgttctc gttcaacaat
    13201 ccgatgggcg cctgccccaa gtgcgacggg ctgggcaaca tcagcttctt cgatccgaag
    13261 cgcatcgtcg ccttccccac gctcagcctg agctcgggtg cgatcaaggg ctgggacaga
    13321 cgcaaccagt tctatcacca gctgctctcc ggcctggcca aacactacga gttcgatctg
    13381 gagcagtcgt tcgagagcct gccggagaaa gttcagcagg tggtgctgta cggttcgggg
    13441 cgcgaggaga tcgcgttcaa ctacctcaac gagcgcggca agctcagcct gcgcgaacat
    13501 cccttcgagg gcgtggtcaa caatctggag cgccgttaca aggagaccga ctcccccacc
    13561 gtgcgcgaag agctggcgaa gttcctcaac agctgcgtct gcaccgaatg ccacggcgcg
    13621 cgcctgcggc tggaggcgcg caatgtgcgc atcgccgaca agaacctgca cgagatcagc
    13681 gcgctgccgc tgaaacagac gctggctttc ttcaagacgc tgacgctctc cggccacaaa
    13741 caggccatcg cggacaagat cgtgctggaa atctccagcc gcctgacctt tttgaacaac
    13801 gtcggcctgg actatctgtc gctggaacgc tccgccgaga cgctgtccgg cggcgaggcg
    13861 cagcgcatcc gtctggcctc gcagatcggc tccggcctga ccggcgtgat gtacgtgctg
    13921 gacgagccct cgatcggcct gcaccagcgc gacaacgacc gcctgctgga cacgctgaag
    13981 atgctgcgcg acatgggcaa cagcgtgatc gtcgtggagc acgacgagga cgcgatcctg
    14041 catgccgact acgtggtgga catggggccg ggcgcgggcg aacacggcgg cctcatcgtc
    14101 gcccagggca cgcccaagga aatcctcgcc aacgaacagt cgctcaccgg cgactacctg
    14161 tccggccggc gcagcatccc gatgcccaag gaatttctca agcccgatcc ggagcgcatg
    14221 ctgaccatca ccggcgcgtc gggcaacaac ctgaaagagg tcaccctcaa tctgccggtc
    14281 ggcctgctgg tctgcgtcac cggcgtgtcc ggttcgggca aatccacgct gatcaacgac
    14341 acgctgtatc ccgtcgtgtc gcagcatctg taccgcagca ccaccgaacc ggccccctat
    14401 gccgagatcg aggggctgga gttcttcgac aaggtcatca acgtggatca gtcgcccatc
    14461 gggcgcacgc cgcgctcgaa cccggcgacc tacaccgggc tgttcacgcc gatccgcgag
    14521 ctgttcgcgg gcgtaccctc ggcgcgcgag cgcggctacg ggccgggccg cttctcgttc
    14581 aacgtcaagg gcgggcgctg cgagtcctgt cagggcgacg gcgtgatcaa ggtggagatg
    14641 cacttcctgc cggacgtgta cgtgccctgc gacgtgtgcc acggccatcg ctacaaccgc
    14701 gagacgctgg agatcaacta caagggccgg aacatccacc agattctgga gatgacggtg
    14761 gaacaggcgc acgagttctt cgcgcccgtg ccggtgatcg cgcgcaagct gcagaccctg
    14821 ctcgacgtcg gcctgggcta catcacgctg ggccagtccg ccacgacctt gtccggcggc
    14881 gaggcgcagc gcgtgaagct gtcgctcgaa ctgtccaagc gcgacaccgg gcgcacgctg
    14941 tacatcctcg atgaaccgac caccggcctg cacttccacg acatcgcgct gctgctcaag
    15001 gtgatccaca agctgcgcga acagggcaac accgtggtgg tgatcgagca caacctcgac
    15061 gtgatcaaga ccgccgactg ggtgatcgat ctcggccccg agggcggcga cggcggcgga
    15121 cgcatcatcg ccgagggctc gcccaaggac atcgcgcaga acaagaacag cgtgaccgga
    15181 aaatacctga agcaggtgat gaaacgctaa ggcagggagg tcaattgcga catcgaatac
    15241 tcgtcacact gaccctgctt gcgatcgcgt cgcccggcgc aatcgccctg ccgcagcaag
    15301 ctccggcgac accctccatc gtcctgcctg ccgatgaagg catccgtttt tcctgcccgc
    15361 cggaacggct cgaagccatt gaatccggca tgtcggccta tctggccaag ctgcatatcg
    15421 cccccgcgct gatcatcgca aggatagacc gcgcgcaggg catcgctgtt tacaccctga
    15481 acacccccgg ggaagacacc aacaccctcg atctgcgcga ccgccccgaa ctgcgcatcg
    15541 aggacgacac cgtcagcttg cctgcggggc atgggaaaaa gaaaagggtc atcaccgtat
    15601 ccaagaagga aatcctgctg gcgctgctgc aacatggccg actcaccgaa ttcaaggaca
    15661 gcgcctgcga tgtacaggca ctgcaggatc acgtcggcat acgccagaac atcgcggcct
    15721 ggggggaaaa cctgaattgg gtatggccga atggcggccc ggcaaagtgg aacgacaagt
    15781 actgggatcg cggcacgccc agagcaggat ttccgctcca tcaggctctc gacgatgtgt
    15841 tcatgaaccc gcgccgatac gccatcggct gttacacctc cagcaagctg ctgatggtgc
    15901 aagggatgct ggattatttt cgccgcatca aacaggatcc cgcgcggcag aagcagctcg
    15961 aacataggct gatgaaggac ggagaaccgc tgaagaacat cgaacccggc aggatgtggg
    16021 acttcgaagc agacttcgat ccgcaggagc tcaatcgccc cggcaaacta ttgagaatcg
    16081 cctacggcat cgcgccgaag aacttcattc ccggcgactg gatccatttt ttcaatagcg
    16141 acccggtcag ctatcagaag accggctatg aaggctcgaa tccggtctat ctgggacgaa
    16201 acaaattcgc cgattacttc aacgaccacg atcactcata cagctaccag cagaagctgg
    16261 atgaggtgta ccaatggcgg aacggcgttt tcagccgttc gcgcgatgcc gcaaagataa
    16321 agccgcttag cgccggtgag attgaacgtt taagccttcc gcccgcccgg ggcggcctcc
    16381 tgacagacat gagggtctcc ccttatttct tcggccatga aagattgcct gaactggaca
    16441 catccccggc tgcggaataa cccactctgc aggggattgc gacggacgaa aaagaggatc
    16501 agtctttggg actgacgctg gttcggtttt ttggattggg tgatacagga agtggtgccg
    16561 cttgtcggat tcgaactgac gacctaccgc ttacaaggcg gttgctctac caactgagct
    16621 aaagcggcga tgctagcaag catgacgctg ctcacattaa aaatggtggg tcgggcgaga
    16681 ttcgaactcg cgaccaacgg attaaaagtc cgctgctcta ccggctgagc taccgacccc
    16741 gtactgcaag aggcgcgaat tataccgata atttgcgccc ggtcaacgct aaatgcacat
    16801 tgatttcatc attcgtttat ttttatattt cctgcgcaag ataaaccgtc ggatcgtcga
    16861 tattcgcggc ggaaaatccg gcacgacgca agcgacagga atcgcacaca ccgcaagctc
    16921 gccccgcctc atccgcctga taacaggaga ctgtctgcgc ataatccacg ccgagcgccg
    16981 tgccctgccg gatgatctct gccttggaca ggttcagcaa tggcgcatgc agcgtcagcg
    17041 catgaccttc taccgcagcc ttggtagcaa gattcgccat gcgttcgaat gcctcgatat
    17101 aagccggacg acaatccgga tagcccgagt aatcgaccgc gttcacaccg atgaaaatat
    17161 cctgcgcctg caacacctcg gcccatgcca gtgcgagcga cagcatgatg gtgttgcgcg
    17221 cgggaacata ggtcgaggga attccggctg ccaattgctg cggcaccgca atgttgttgt
    17281 cggtcagcga cgacccgccg aatccggtca ggtcgatgta caccacgcgg tgttcatgcg
    17341 cgcccagcat gctggcgacg cgctgcgcgg cggcaagctc ggaatggtgg cgctggccgt
    17401 aatccacact cagcgcatag caggtatatc cctgcgcacg cgccatcgcc agcaccgttg
    17461 cagaatccag cccgccggat aacagcacca ctgcgttctt cgtcgtcatc gccccgcctc
    17521 gttcggccaa agtatcttgt gcaactggac ctgcatgcgc accggcagac ggtcgcgcag
    17581 gatccactcg gccagctgcg tcgcactcag cgacgcatgg gcgggcgaga aggtcacttc
    17641 gcatctctcc gcgaggcggt gttgagccag cgtctgtttg gcccacaggt agtcgttctc
    17701 gtcgcacagc acgaatttta tttcgtcatg cgcgttcagc aacgccagat tgtcccatcg
    17761 attcttgccc gcttcgccgg aagcgggtgt cttaatgtcc agcacgcgca ttacccgcgc
    17821 atcgacgccg ccgatgtcca gcgcgccgct ggtttccagc gacacctcat aaccggcatc
    17881 gcacagcgcc gtcatcagcg gcagagcatt cttctgcgcc agcggttctc cgccggtgac
    17941 gcagacatag cgcggcgcat aactggcgac ctgttcgagg atttccgtca ggctggcatt
    18001 gcgcccgccg gtgaaggcgt agctggtatc gcagtaggtg cagcgcaggg ggcagccggt
    18061 caggcgaatg aagaccgtag gcaggccgat gcggcgggtc tcaccctgca acgagaagaa
    18121 tatttcgctg atgcgcagtt gcgcatcctg agtgacgtgc gacatgggaa ttacttcttg
    18181 gaagcgagtt gcttcttggc ttgggccgcg acatcgctgt ccggatattt ggagatgagc
    18241 agcttgagtg tcttctgtgc gttctcgccc tgcttcaact cctgctggca tccggcgatg
    18301 ttgaacagca cctcggcatc tttgggctgc gaaggataat ccttgcgcaa gacgcgatag
    18361 gtctccagcg ccgccttgta gttcttcatg gcgtactggg cattgcccag ccaataagac
    18421 gcattgggca catacaccga ttccggatat ttcctggtga actcctggaa cgccttgatg
    18481 gcatccagat ggttgcccgc cttgaacagg gcataggctg cttcataggc gcgattctcc
    18541 tgcgccgtgt caccgctgtc ggacttggcc gcatcgcctt gcccgaccgt atctgaggat
    18601 tcgaaatgac gcacgcgcgt atccaaatcc acataaaaat ctttttcgcg tttctctgca
    18661 tcctgcagac cgcgaactaa ctcttcattc tgtccgcgca atttgcgcat ttcgccgttc
    18721 agcgtttcaa tttgtgtcag caagtccagc atggacttgg tttgctgctc gcttgccgct
    18781 tccactttgg acaaacgcgc ctcaagcagc tgaatctgtt tgtgtgcttc gtcgtccgaa
    18841 aacaaccccg cctgcgcggg cgcaacaaaa aacagactca gcaacaggag agcgcgctgc
    18901 cttaacatgg tttacttcgc gtagttcaga tcggtgcgac gattctgagc ccaggaagcc
    18961 tcgtcatgac cggaagcttt tggcttctct tcgccgtagc tgacgctatc gagctgagca
    19021 gtttttgcac cgctcacttc cagcattttc ttcacgccat cggcacgacg ctgacccaga
    19081 gccaggttgt actcgttgct gccgcgctcg tcgcagttgc cttccagacg caccttgcgg
    19141 tcggcatggt tcgccagata ttgaccgtga gcctgaatca tcggcttgtc ggcttcctga
    19201 acgacggaaa cgtcgaatgg atagtaggcg ctacgcttgg ccaggatgct ggctgggtcg
    19261 ttcagtgcat caacgctgga tgcagatgca gcctgagtgg atgcagcagt gcctgctgca
    19321 gaaccggctg gagctgctgt ggtttctgct ggcttgttgc tggcgcaagc ggccaacaga
    19381 ttgaccaaca cgagactgat aacgagtttt ttcatagtgc acccctttgc taggttagtt
    19441 aaggattggg tccccacacc ggttcacgtg cgtcaccgcc ttgtgtgaac atgtgctgct
    19501 tgacgcgacc gtcgctggaa acggtcgcca atataccacg accgcgcgct tcactggcaa
    19561 acaggatcag ttttccattg ggagcgaaac tgggtttttt ctcccagcca ccctcggtca
    19621 gaatctccat ctgcccggtc tgaaaatcct gcacggcgat gtagaacttg ccgctgctgc
    19681 gatgtgcaaa cacaaagctg ctgccatccg gactgtagcg cggcgagaag ttatttccct
    19741 caccgaaggt catgcgctgc tcgggtccac cgttcaccgg catgcggtaa atctgcacgc
    19801 tgccgccacg atcggaggag aacagcaggt attgcccgtc cggcgaaaaa ttgggttcgg
    19861 tatcgatcac gccgctgaag gtgatgcgcc gcagattgct gccatcggga cgcaccagat
    19921 agatctgcga accgccgtcg cgcgtcagca cgacagccat ctgcttgcca tccggcgacc
    19981 aggccggcgc gctgttgctg ccgcgaaaat cggccagcac cttgcgctga ctggtaagaa
    20041 gcgattgcac atacaccacg gcatggccgg tctcgaagct cacataggcc agatggctgc
    20101 catccggcga ccaggatggc gacatgatgg gttcgttatg cgccaggatg acctgctcgt
    20161 tatagccgtc gctgtctgcc acgatcaggc ggaagttgct cccctgccgg ttcacatagg
    20221 cgatgcgggt gctgaacacg cccttgtcgc cggtcagctt ctcgtaaatc atgtcggcga
    20281 tgcgatgacc gaccgcacgc gcctgcccat cgcccgccga cacggcctgt gcgatgagtt
    20341 cattctgttt gaccacatcg aacagacgga agtgcgcctc gatgcggcca ttgggctggg
    20401 ccgtcacccg tccgatcgac agcgcatccg caccgcgcac ctgccagtcc gcatagctca
    20461 cttgcgacgg ctcatccaga aacttgcccg ccagatccac catgcggaac aggccgctgc
    20521 gctgcagatc tccggtcacc acctcgttga tggttttcga cagctcgctg tcgccgccga
    20581 agggcacaag ggccaccggt atctgttttt cacccgcacc gatgatctcg atattcagcg
    20641 ccgccgatgc aacagacaac tgcaccaaca acagcaacgc caccattcca cgtattacct
    20701 gcatcaacac tactccttcg gcctgaagcc caatttcatt tcacgaaact tgttaaacaa
    20761 cgccgcatcc ggcggcagcg gcaagggctg cgccttcagg atcgcacgct cgaccgcatt
    20821 gtcgtaggcc gcattgccgc tggacttgag caggcgcgga ttcagcaccg agccgcccgg
    20881 cagcaccgtc acggcgaact cggcgcgcgc gtcgtccggc acacccggcg gcatcacgat
    20941 gcgactgcgt atcttggcgg aaatcttgcc ggtatattca tccaccatcc ttcccgtcgc
    21001 cgcagcgcgc tcggtctgtt ccgcctgttc gcgcagcgcc tgctcgtcca cctgcggctc
    21061 tttgactttc ttctcgggct ttttctcggg cttcttctcg accggcttgg ccttcttgtc
    21121 cggcaacgcg atctccggct gtttctccgg cgcgggagga ggggcaggcg cttcgacctt
    21181 ctgctcgact ttttcctcga ccttgggctt ggcggacggc gcagacggga tgctgtccca
    21241 caactccacg ctcatcgcgg ccacgggttc cgtctgccag gagaaaccga aatacagcag
    21301 cgcgaagaat gcgccatgca ccgccaaggc gagtatcccc gccggcagtc tgttagcttc
    21361 gtgataaacg acaggactca tttccggttc tgcgtcagca acccgacctt cttgatctgt
    21421 tgctgctgca acaagtccat cacattgatg acttcctcat agcgcacatt cttgtcggcg
    21481 gaaatgacca ccgcctgatc ggccagatcg ccctgccgcc ccttcaggat cgcgatcagc
    21541 tcgtcgcgcg tgacgatgct ttccttgctg cctctggaat ggtcgcgcag cgccagcgag
    21601 ctgtctttct tgatgatgac ttccaagggt gccaccggcg gcgccagcga ctggccgacc
    21661 tgcggcagat cgatctgccc gggattcatc atcggcgcgg tgaccatgaa gatcaccagc
    21721 agcaccagca tcacgtcgat gtagggcacg acgttgatct cgttcatcag acgattgttc
    21781 gagcggcgat tagtcctcat gactgactct cctctgcccg gtcatttacg gctgacgctg
    21841 caacacgttg gagaactctt cggtgaagct ctcgaaacgc gtggacaggc gattgatatc
    21901 gtgcgaataa cggttgtagg cgatcacggc ggggatcgcc gcgaacaggc ccatcgccgt
    21961 ggccaccagc gcctcggcga tgcccggcgc gacatgggcc agcgtcgcct gcccgacatt
    22021 ggcaagaccc cggaacgcat tcatgatgcc ccaaaccgtg ccgaacagac cgacataggg
    22081 gctgaccgaa ccgaccgagg cgaggaaggc cagatgcgat tccagcctgt ccatctcgcg
    22141 ctgataggtc gcgcgcatcg cgcggcgtgt gccgtccatc accgcactga catccccgcc
    22201 gtgctgctgt ttcttcagct tcacgaattc ggcgaagcct gccgcgaaga tgcgctccag
    22261 cgcgccctgt tcgccgtgca gggaactgcc gctcgagcgg cgatacagct cgttcagatc
    22321 atggttgcgc cagaactcgt cctcgaacgc ctcggtcagc ctgacctcgg cgcgaatcga
    22381 aaacatcttc aggaagatgt accaccagga catcatcgac accagcagca gcaaccccat
    22441 caccagttgc accagcacgc tggcgttgct gatgagatga acaaaagaca gatcctgcgt
    22501 cacttccatt gcatttcctt cccgttaaat cttcaaaacc tgctatccac gaaaaacact
    22561 aaactcacga aacaaaatca atgctcatga accacccctc tatttttcgt gtcttttcgt
    22621 gacgttcgtg gacaaatgca gtttccccat taaatcttcc agtgtttgct caatacctcc
    22681 ggcacgctga ccggcttgaa actctccacc ccgacgcaca ccagatgcac ctcggccacg
    22741 gtcatcagct cgtcgccccg cgtcaccgtc tgctccagcg tgatgcggct gcgtccgacc
    22801 tccttcaact gcgcggtcac gtccagcatg tcgtcgagca atgcaggcct gagataattc
    22861 agctccagct tgcgcaccac gaacatcacg cccagctcct gcatcaatcc ggcgttgctg
    22921 taaccgaacg tgcgcaacca ctcagtccgc gcccgctcca taaagttcac ataattcgcg
    22981 tgatacacca ccccgcccgc atcggtatcc tgataataca cacgggtagg ccagctaaaa
    23041 atcttgtgtt tactcatcca gcaagtctcc catcaccggg ttgctcgcga ccgccagccc
    23101 gaaatgtttg taggcgttcg ccgtcgccat gcggccgcgc ggcgtgcgtt gcagatagcc
    23161 ctgctggatc agatagggtt ccagcacgtc ctcgatggtg tcgcgctctt cgccgatcgc
    23221 ggcggccagg ttgtccacgc cgaccgggcc gcccatgaat ttctcgatca cggtgagcag
    23281 cagcttgcgg tccatgaggt ccagcccgcg cgtatccacg tccagcatgg tcagcgcggc
    23341 atccgccacc tcgcgactcg catcgccgtc cgccttcacg tcggcgaagt cgcgcacgcg
    23401 acgcagcaat cggttggcga tacgcggcgt gccgcgcgag cgcttggcga tctccagcgc
    23461 gccgtcctga gacaggttca tctccagcag accggacgag cgcatcacaa tgcgctgcaa
    23521 ttcctccgga gtgtagaact ccagccttgc tacgatgccg aagcggtcgc gcagcggatt
    23581 ggtcagcatg cccgcgcgcg tggtcgcccc caccagcgtg aacggcggca ggtcgagcgt
    23641 caccgagcgc gccgccgggc cgtcgccgat caggatgtcg atctggtagt cctccagcgc
    23701 gggatacagg atttcttcca ccaccggaga cagacggtgg atctcgtcga tgaacaaaac
    23761 atcattcggt tcgagattgg tcagcagcgc ggccagatcg cccgcgcgtt ccagcaccgg
    23821 gccggaggtg tggcggatgt tcacccccat ctcgcgcgcg atgatctgcg ccagcgtggt
    23881 cttgcccaga cccggcgggc cgaacagcag cacgtgatcg agcggctcgc cgcgccgctt
    23941 ggccgcctcg atgaatatct ccagctgccc gcgtatcttc tcctgaccga catactcgtc
    24001 aagctgcttc ggccgcagcg cgcgctccag cgcctcctcc tgacgcgagg cgggagcggc
    24061 cgaaatgatg cgagatgcgg cggaaaggtc gtcgttgtgg atcatgcctt ggataataac
    24121 ttaagtgact tcacgatttg ga
//
LOCUS       DASUIV010000157         5021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_36653, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000157 DASUIV010000000
VERSION     DASUIV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36653"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFW59_05885"
     CDS             complement(<1..239)
                     /locus_tag="VFW59_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363777.1"
                     /translation="MSTKPSFNPAADERTGVADRRLFDDIRARIARVPRVRLAITPTP
                     MIEAPNLAQHLGGPRIFIKRDDLTGIAFGGNKLRNL"
     gene            complement(443..1420)
                     /locus_tag="VFW59_05890"
     CDS             complement(443..1420)
                     /locus_tag="VFW59_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5363778.1"
                     /translation="MRISVLSAIAGLLMLAGSVSAQESWPNKPISLVLPYAVGGGADL
                     MARSLAESMRKTLGQPVNVLNVAGAGSVLGSRQVASAAPDGYTLLMNHMGLATAPALY
                     KNLQFDPVKSFEHIGLYAEIPMLIVAGKDFPANNAKELIEYVRKNKDKVTMASSGMGS
                     GTHLCAMLFEKAVGAKVTMIQYKGSAPAYMDIQSGRVDLMCDSTGGSVAQVKGGGVKA
                     FMVTGNKRIQSLPNVPYSAEAGLKDLGLMTVWYGLFAPAGTPRPIVERLSAAMQAAVR
                     DPVVAKLMDNWDATLYEPKYAVPAAMHDKMSSQVEMWTRLIQNAGLAPQ"
     gene            1629..2621
                     /locus_tag="VFW59_05895"
     CDS             1629..2621
                     /locus_tag="VFW59_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5363779.1"
                     /translation="MNLLRIQAFLTVAETRNISRAAAQLEVAQSVLSRHIRAMEEELG
                     CRLFERTGRGVALTDAAERLAPRLRSALDEMRRATAEAVETADQPSGVVRLGVVPAAA
                     HPLVGLLYQRVTSRFPRIALQFVEGFSNPLEEKLAVGDIDLAVINRYGRAQRRAEERL
                     CVVDSYVIGPPGAFQPKGRDMTFKQLAELPLVLAARPNSLRLELDQLCQRVGVQLRVV
                     VESDSLLIMKDLIVRSDLYTLLPRQAVYEELEHGLLDAARLITPGLPRTLSLLSAPRG
                     PTSAATRAVSREIREIVQTSMVLAPSGIEPRVALVAAGGPRRRVEVGISQRDCS"
     gene            complement(2710..2835)
                     /locus_tag="VFW59_05900"
     CDS             complement(2710..2835)
                     /locus_tag="VFW59_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363780.1"
                     /translation="MTMGVDERCPSWAMSKPGWWREDDVFCHSLANVVDVAGSRE"
     gene            complement(2878..4413)
                     /locus_tag="VFW59_05905"
     CDS             complement(2878..4413)
                     /locus_tag="VFW59_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5363781.1"
                     /translation="MNTENSKKSAPRIALLGLHLEANGFAPPTQRKDFENQCWEEGEA
                     ISVLARQVSHLPAELPGFYARMDATGPWTAVPVIMISATPGGPIMKDVFQDFLDRVQR
                     RLLAALPVDGVYVASHGASSATDDLDSDGTLLALIRDIVGPTVPVVVTHDLHCNVSER
                     LVQCADALIAYRTNPHVDQRERAAEAADLMRRLLGGLKTKKAFIRMPIAAPTVTLLTA
                     QGPYADLIKLGQELTQPPVLNVSVTGGCVFGDLPKCGMTINVTADNDQAAADAVARQI
                     ARAAWADRDRYFRKLTSLDEAVNLARRAASEDSLPIILADVADNPGGGGRGNTVWLLE
                     ALHAAKAAGVVMGMFIDSGLVEDALLVGEGAHFQAVFNRTESEFSKRFECGATVEKIT
                     DGIAVGRRGILAGMQFNLGPTALLRLDGSGLRVAVASLRQQMADPRMLEMLGIDIAKI
                     KCLVVKSRGHFRAGFDEFFTPEQIYEVDTPGLTSPVLGNFAWKHMKRPIWPLDAETTW
                     NEY"
     gene            complement(4572..>5021)
                     /locus_tag="VFW59_05910"
     CDS             complement(4572..>5021)
                     /locus_tag="VFW59_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363782.1"
                     /translation="ETQPVVIAAALRAAGFTVDLQTMDWQTLVTRRANQKPPAEGGWN
                     IFASNSAVANTFDPLSSAQVPANGKKAWFGWPDVPEIEKLRTQFAHASSESERKKITE
                     QLQKLVIDEGVIVPLGQFVIMTAYNKKLTGVLDASLPLFWNIKKAAK"
BASE COUNT         1065 a         1504 c         1405 g         1047 t
ORIGIN      
        1 aggttgcgca gcttgttgcc gccgaaggcg atgccggtga ggtcgtcgcg cttgatgaaa
       61 atgcgcggac cgcccagatg ttgggccagg ttcggcgcct cgatcatcgg cgtcggcgta
      121 attgcaaggc gcacacgcgg cacgcgtgcg atgcgcgcgc ggatgtcatc gaacaggcgg
      181 cgatcggcca cgccggtgcg ctcgtcggcg gcgggattga agctaggctt ggtggacacg
      241 gtcattccag ctttcggagt agagatgggt tctgtcgcct tgtgcgggca ggaaggatcg
      301 gtacccttgc aacagcgcgc gtaacgcgaa attcttgatg tcggaaattg ctgacaggca
      361 catggcccaa ggtcaggagc agacccaccc gcgcgtggcg ggaaggtcat cggtccacag
      421 tagtctgttc gggcaagctg aattactggg gggcgaggcc ggcgttctgg atcagacgcg
      481 tccacatctc aacctgcgag gacatcttgt catgcatggc tgcgggtacg gcgtacttcg
      541 gctcatagag cgtggcgtcc cagttgtcca tcagcttggc gactaccggg tcgcgtaccg
      601 ctgcctgcat cgccgcggaa aggcgctcga cgatgggtcg gggagttccg gccggtgcga
      661 acagtccgta ccacaccgtc atcaacccga gatccttgag gccggcctcg gccgagtagg
      721 ggacgttcgg caggctttgg atgcgcttgt tgccggtcac catgaaggcc ttcacgcctc
      781 cgccttttac ctgcgcaacg ctgccgccgg tactgtcaca catcagatcg acgcggccgg
      841 actggatgtc catgtaggca ggtgccgagc ccttgtactg gatcattgtg accttggcgc
      901 cgacggcctt ttcgaacagc atggcgcaaa gatgggttcc gctacccatg cccgaggacg
      961 ccatcgtgac cttgtcctta ttcttgcgga cgtattcgat gagttccttg gcgttattgg
     1021 cggggaaatc cttgccggcg acgatcagca tcgggatctc ggcgtacaag ccgatgtgct
     1081 cgaaactttt caccgggtcg aattgcaggt ttttatacag tgcaggcgcg gtggcaaggc
     1141 ccatgtggtt catcagaagg gtgtagccgt ccggggcggc gctggccacc tgccgcgaac
     1201 cgagcacgct gccggcacct gccacgttca acacgttgac cggctgtccc agcgtcttgc
     1261 gcatcgattc ggccagcgac ctggccatca ggtcggcgcc accaccaacg gcgtagggca
     1321 gcaccaacga gatgggtttg ttcggccagg actcctgggc cgaaacagaa ccggcaagca
     1381 tcagaagtcc agcaattgcg cttaaaacag aaatccgcat actccgtctc ctttaaatgt
     1441 aagaaatttc gtcataacta atgccctgtc tgccaaggaa ggtctgcata agacagcctg
     1501 atgcaggcat gtcttatgca gacccttttc aggtggctcg cgaaggcaag tatagaaagt
     1561 gatcatgatg caaaaaagag atcagcaagc atttctgtta tgcttttcaa gcacttcttt
     1621 ggaatcccat gaacctgctc agaatccagg ccttcctcac cgtggcggag acacgcaaca
     1681 tctcgcgcgc cgcggcgcag cttgaggtgg cgcagtcggt gctcagccgc cacatcaggg
     1741 cgatggaaga agaactcggt tgccgcctgt tcgagcgcac cggccgcggc gtcgcactaa
     1801 ccgacgcggc agagcggctt gctccccgcc tgcgttccgc cctggacgaa atgcggcggg
     1861 ccacggccga ggccgtggaa acggccgacc agcccagcgg cgtggtccgg ctgggcgtgg
     1921 tgcccgccgc ggcgcatcct ctggtgggac tgctctacca gcgcgtcacc tcgcgctttc
     1981 cgcgcattgc cctgcagttc gtcgaggggt tcagtaatcc actcgaagag aagcttgccg
     2041 tcggtgacat cgacctcgcc gtgatcaacc gctacgggcg cgcccagcgc cgtgccgagg
     2101 aacggttgtg cgtggtggac agttacgtga tcggcccgcc cggggccttt cagccgaagg
     2161 gtcgggacat gacgttcaag caactggcag agttgcccct ggtgctggcg gctcgcccca
     2221 acagcctgcg cctggaactg gatcagctgt gccagcgcgt cggcgtgcag ctgcgtgtgg
     2281 ttgtggaatc tgactcgctg ctgatcatga aagacctgat cgtgcgaagc gatctctata
     2341 ccctgctgcc acggcaagca gtgtacgaag aactggaaca tggcctgctg gatgcggccc
     2401 gcctgatcac gcctggcctg cccaggacac tgtcactgct ctccgcgccc aggggtccaa
     2461 cctcggcggc aacacgcgcc gtgtcacgcg agatacgcga gattgttcag acctcgatgg
     2521 ttctcgcacc gagtggcatt gaacctcgag tggctttggt ggctgcgggc ggcccccgtc
     2581 gacgcgtcga ggtgggcatc tcccagcggg actgctcctg atcccacgtg cgaaaacgga
     2641 tactgaaccg ttgccagcat tgggatcgca aggatttagc ttgggtcagt ccatcgtcgg
     2701 cacttgggtc tattctcgac tgcccgcaac atccaccaca ttagccagac tatgacaaaa
     2761 aacatcatcc tcgcgccacc agcccggctt gctcatcgcc cacgacggac aacgctcatc
     2821 gactcccata gtcaagaatt ttctcccgcc ttgtcataac ccgagacagg cgttcactca
     2881 gtattcattc catgtcgtct cggcatcaag cggccaaatc gggcgtttca tgtgcttcca
     2941 cgcgaaattt cccaatacag ggctagtcaa accaggcgta tctacttcat atatctgttc
     3001 cggcgtgaag aattcgtcaa acccagcccg gaaatgaccg cgacttttca ccaccaggca
     3061 ttttattttg gcaatatcga ttccaagcat ttccagcata cgcggatcgg ccatctgctg
     3121 acgcaagctg gcaacagcca cacgcaatcc agatccgtca agcctcagca gtgccgtagg
     3181 ccccagatta aattgcattc cggccaatat accccgtcgg cccacggcga tgccatcagt
     3241 gatcttttcc actgttgcac cacactcgaa acgctttgaa aattcgcttt ctgtacgatt
     3301 gaatacggcc tgaaaatgcg cgccttctcc gacaaggagg gcgtcctcaa ccaggccgga
     3361 atcaataaac atgcccataa ccaccccagc cgccttggcc gcgtggagtg cctccaaaag
     3421 ccatactgtg ttgccacgcc cgccgccgcc cggattatct gccacatcgg ccagaataat
     3481 cggcaggctg tcttcagacg cggcccgtct tgccagatta acagcctcat ccagcgacgt
     3541 cagtttgcga aaataacggt cccgatcagc ccaggctgcg cgagcaattt gccgtgccac
     3601 agcgtcggca gccgcctgat cgttatctgc ggtcacatta atggtcatgc cgcattttgg
     3661 aagatcgcca aacacgcatc cacctgtgac agacacatta agaacgggtg gctgtgtcaa
     3721 ttcctggccg agcttgataa gatcggcata cgggccttgt gcagtcaaca gcgtcacagt
     3781 gggtgctgca atcggcatcc gaataaatgc cttcttcgtc tttaatccgc cgagcagccg
     3841 acgcatcaga tctgctgcct cggctgcgcg ttcgcgctgg tcaacatggg gattggtgcg
     3901 ataggcaatc agtgcgtcgg cgcactgaac aaggcgttcg gaaacattac agtgcaggtc
     3961 gtgcgtcact accaccggca ccgtgggccc gacgatatcc cggatcagcg ctagcaaagt
     4021 accgtcactg tccaaatcgt ccgtagccga tgaggcccca tggcttgcaa catacacgcc
     4081 atccacagga agcgcggcaa gcagcctgcg ctgtactcga tccaaaaaat cctggaatac
     4141 gtccttcata atgggcccac caggcgttgc actgatcatg atgacaggca ccgctgtcca
     4201 tgggcctgtt gcatccatgc gcgcatagaa acccggcaac tcggcaggca agtgacttac
     4261 ctgccgcgcc aggacactga tcgcctcacc ctcttcccag cactggttct caaaatcctt
     4321 ccgttgcgta ggcggcgcaa atccatttgc ctccaggtgc aggcccagca gcgcaattcg
     4381 cggcgccgat ttcttactgt tttcggtgtt catgaaatag tctttttaaa gaatcaggta
     4441 aagataatcc ctgaaaatag caaccaaatt atttcgcatt aagcaacaag cgcaggaatt
     4501 gaccacggta tttagtatcg tggggaagcg tggacaacag ccgtcgtgca atataagcta
     4561 gcatgtagac ctcacttagc cgccttttta atattccaga atagaggaag cgacgcatcc
     4621 agaacacccg tcagtttttt gttatatgca gtcataatga caaattgccc aagcggcacg
     4681 atcacaccct catcgattac gagtttttga agttgttccg ttattttttt acgctcggat
     4741 tcgctggagg catgcgcgaa ttgagtacgc aacttctcaa tttcaggcac atcaggccag
     4801 ccgaaccagg ccttcttgcc gttagcagga acctgggcag agcttagtgg atcgaatgta
     4861 tttgcaacgg cactattaga agcaaaaatg ttccagcccc cttcggcggg cggcttttgg
     4921 ttagcacggc gggtaaccag cgtttgccag tccattgtct gcaaatcaac agtgaagccc
     4981 gcagcacgca acgcggccgc gatcactaca ggctgcgttt c
//
LOCUS       DASUIV010000158         6132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1060320, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000158 DASUIV010000000
VERSION     DASUIV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060320"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFW59_05915"
     CDS             complement(<1..514)
                     /locus_tag="VFW59_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5363783.1"
                     /translation="MDITELLAFGVKHKASDLHLSAGLPPMIRVHGDMRRINLPAMEH
                     KEVHALVYDIMNDGQRKFYEENKEVDFSFEVPGLARFRVNAFIQNRGAAAVMRTIPSK
                     ILSLEDLKCPKSFEGISEYPRGLVLVTGPTGSGKSTTLAAMVNHRNENEMGHILTVED
                     PIEFVHESKKS"
     gene            569..1270
                     /locus_tag="VFW59_05920"
     CDS             569..1270
                     /locus_tag="VFW59_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5363784.1"
                     /translation="MTTIASNMQATRAAIAAAASAAGRRAEDVILLAVSKTFPAEAVR
                     EAYQAGQRLFAENYLQEGVDKIAELQELAIEWHFIGPIQSNKTRPIAEHFAWVHSVDR
                     LKIAERLSAQRPTDLPPLQICLQVNVSNEPSKSGVAPEQTAALAQDIAALPNLRLRGL
                     MTVPAPSDDIPAQRAAFAQLRELFEQLNRDGLRLDTLSMGMSHDFPAAIAEGATLVRI
                     GSAIFGNRTYPKETT"
     gene            1267..2088
                     /gene="proC"
                     /locus_tag="VFW59_05925"
     CDS             1267..2088
                     /gene="proC"
                     /locus_tag="VFW59_05925"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5363785.1"
                     /translation="MKICFIGGGNMAAALIGGMTSKGFKADQISVVEINPDNRAKLQR
                     DFAVRAVAQLAEGVAGSDVVVFAVKPQQLREVAQQAGPLLDGKLLISIAAGIRASDLA
                     RWAGNQAVVRAMPNTPALVQSGMTGLYAMPEVSAAQREQAQEILISVGETLWLDDEAM
                     VDAVTAISGSGPAYVFYFIEALQKSAQELGFNADDARRLSLATFLGASKLAAASEEEV
                     GVLRARVTSKNGTTERALLSLAANRVDEHIAQAAQAAAARAREMADELGNQGEPA"
     gene            2085..2651
                     /locus_tag="VFW59_05930"
     CDS             2085..2651
                     /locus_tag="VFW59_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5363786.1"
                     /translation="MMQEALMFLFDVLLQPFAAILLLRFHLQWLRAPLRNPIGEFVMV
                     LTDFLVLRTRRFIPSFRGLDSATLLLALLFELLYLIGLLWAQGYPFSAFPLPGLLFLS
                     AVKLLKTSIYLLMAAVFAQAILSWLNPHTAAAPLLDAVTRRFLQPLRRIVPMLGNIDL
                     SGLLLFIICQLMVIVPITMLERIAARLL"
     gene            2652..2939
                     /locus_tag="VFW59_05935"
     CDS             2652..2939
                     /locus_tag="VFW59_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEX5363787.1"
                     /translation="MTEWYRRQGEVLTLTLHIQPGAKRSELAGLHGEALKIRLAAPPV
                     EGRANEALLKFIAKLFEVPVRQVELKQGGQSRHKVIAITGSNVDPLSLLDS"
     gene            complement(2970..3875)
                     /locus_tag="VFW59_05940"
     CDS             complement(2970..3875)
                     /locus_tag="VFW59_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5363788.1"
                     /translation="MEQRSLSADDYILFAAIVEQGSLVRAAESLAMPKATVSRRLTNL
                     ETALGQRLLIRTTRRLTLTEFGQEFLDHCRRIAEEVATAQDFVRSQEARPRGHLRVSM
                     PGDYARQYFSRAIATFIETYPEIQLDLDLSSRRVDLIGERFDLAIRMGTLENDATLVA
                     RKIDEHLLGLYASPIYLALHPAPRHPDDLQHHAAVRLLSARGAAIPWKLVRGKEVWEG
                     VPPGRLTLNSPDMIQQLLLDGAGIGAVPCRLAAEEVRFKRLVRVLPEWSLPAVPAWAV
                     MPMRRYLPVKTRAFLSHLELFLGNG"
     gene            3965..4825
                     /locus_tag="VFW59_05945"
     CDS             3965..4825
                     /locus_tag="VFW59_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5363789.1"
                     /translation="MNTATTTTLNHSRSIERIVEGVATSDGAGVKLTRVLTGKLQRRL
                     DPFLMLDAFGSDDPDDYIAGFPDHPHRGFETVTYMLRGRMRHRDSAGHEGLLGDGGVQ
                     WMVAGRGVVHSEIPEQEDGVMEGFQLWLNLPAQSKMTDPWYRDYASVAIPEYVTQEGV
                     TVRVIAGSSNGVAGAVSREITEPLYLDIHLPAGTGFSTAIPASHNAFIYVYRGAVKVG
                     GVQVDSQRMGILSNSADGLSLSASGDARLILVAGKPLNEPIVQYGPFVMNSQEEIRQA
                     LDDFRAGHFA"
     gene            4890..5438
                     /locus_tag="VFW59_05950"
     CDS             4890..5438
                     /locus_tag="VFW59_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX5363790.1"
                     /translation="MSTPITRYSNTAIALHWLMALLIFAAFPLGLYMHDLPLSPTKLQ
                     LYSYHKWIGITVLLLAVFRVMWRATHTPPALPDSLPRWQQLASHATHQFLYVLIFAVP
                     LSGWLMSSAKGFKTLWFGVLPLPDLVGKDKALGELLGNLHASLNYLLLAMVVLHVAAA
                     LKHRLIDRDDVMNRMLPTGERK"
     gene            5435..6007
                     /locus_tag="VFW59_05955"
     CDS             5435..6007
                     /locus_tag="VFW59_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5363791.1"
                     /translation="MNILKYLAVCGLTLACSQVSAQEFKQVLANESSVTFGYKQMGVP
                     MDGKFNRFAAQLAFDPSRVANAQARIDIDVASIDTGSTEANDEVVGKLWFNAKTYPAA
                     SFTSAGLKALGGNRYEARGKLSIKGRTLDIVAPVTFQATGSRGSFDGSFTIKRLDYAI
                     GEGEWTDVGTVANEIQVKFHLVVNATPSKK"
BASE COUNT         1239 a         1924 c         1820 g         1149 t
ORIGIN      
        1 gcgatttctt ggattcatgc acgaattcga tcgggtcttc caccgtcagg atgtggccca
       61 tctcgttctc gttgcggtgg ttcaccatcg ccgccagcgt ggtggacttg cccgaaccgg
      121 tcgggccggt caccagcacc aggccgcgcg gatactcgga aatgccttca aaactcttcg
      181 gacatttcag atcttccagc gacagaatct tggaaggaat ggtacgcatg accgcagccg
      241 cgccgcgatt ctggatgaag gcattgacgc ggaaacgcgc cagaccggga acctcgaagg
      301 aaaagtcgac ctccttgttc tcctcgtaga acttgcgctg accatcgttc atgatgtcgt
      361 ataccagcgc atggacttcc ttgtgctcca tcgcgggcag attgatgcgg cgcatgtcac
      421 catgcacacg gatcatcggc ggcaggccag cggagagatg caggtcggaa gccttgtgct
      481 tgacgccgaa ggcgagcagt tcagtgatat ccattataat cttcccgatt caatgagacg
      541 gcctgaatgg ccgctagtgc gccggattat gacaacaatc gcttccaaca tgcaagccac
      601 gcgcgccgcg atagccgcag ccgccagcgc ggcgggtcgc cgcgcagagg atgtcatcct
      661 gctggcggtc agcaagacct ttcccgccga ggcggtacgc gaggcctatc aggccggtca
      721 gcgcctgttc gccgaaaact atctgcagga aggcgtcgac aagatcgccg aactgcaaga
      781 actggctatc gagtggcatt tcatcggccc gatccagagc aacaagaccc gcccgatcgc
      841 cgaacatttc gcctgggtac acagcgtgga tcgcctcaag atcgcggaac gcctgtcggc
      901 gcagcgccca accgacctgc cgccgctgca gatctgcctg caggttaacg tcagcaatga
      961 acccagcaag agcggtgtag cgccggaaca gaccgccgca ctggcacagg acatcgccgc
     1021 gctgcccaat ctgcgcctgc gcggcctgat gaccgtcccg gcacccagcg acgacatccc
     1081 ggcacagcgc gcggccttcg cacagctgcg cgagctgttc gagcaactca accgcgacgg
     1141 cctgcgactc gacacgctgt cgatgggcat gtcgcacgat ttcccggcgg ccatcgccga
     1201 aggcgctacc ctggtgcgca tcggctcggc cattttcggc aaccgtactt atcccaagga
     1261 aacaacatga aaatttgctt catcggcggc ggcaacatgg ctgcggcact gatcggcggc
     1321 atgaccagca agggcttcaa ggccgatcag atcagcgtgg tggaaatcaa cccggacaac
     1381 cgcgcgaagc tgcaacgcga cttcgcggta cgcgccgtgg cgcaactggc cgaaggcgtg
     1441 gcgggcagcg atgtcgtggt gttcgcggtg aaaccgcaac agctgcgcga ggtcgcgcaa
     1501 caggccgggc cgttgctgga cggcaagctg ctgatctcca tcgccgccgg catccgtgca
     1561 agcgacctgg cgcgctgggc gggcaatcag gccgtggtgc gcgcgatgcc caacaccccc
     1621 gcgctggtac agagcggcat gaccggcctg tatgccatgc cggaagtcag cgccgcgcaa
     1681 cgcgaacagg cgcaggagat tctgatatcg gtgggcgaaa cactctggct ggacgacgaa
     1741 gccatggtgg atgcggtcac cgcgatctcg ggcagcggcc cggcctatgt gttctacttc
     1801 atcgaggcgc tgcagaaatc cgcgcaggaa ctgggcttca atgctgacga tgcccgccgc
     1861 ctgagtctgg cgacctttct cggtgccagc aagctggccg ccgcgagcga agaagaggtc
     1921 ggcgtgttgc gcgcgcgggt cacgtcaaag aacggcacca ccgaacgcgc gctgctgagc
     1981 ctggccgcca accgcgtgga cgaacacatc gcccaggcgg cacaggcggc ggcagcgcgc
     2041 gcgagagaga tggccgatga actcggcaac caaggagaac cagcatgatg caagaagccc
     2101 tgatgttcct gttcgatgta ctgctgcagc ctttcgcggc catcctgctg ctgcgctttc
     2161 atctgcaatg gctgcgcgca ccgttgcgca atcccatcgg cgagttcgtc atggtgctca
     2221 ccgattttct ggtgctgcga acccgccgct tcatcccctc attccgcgga ctggacagcg
     2281 cgacgctgtt gctcgcgctg ctgttcgaac tgctctacct gatcggcctg ctgtgggcgc
     2341 agggctatcc tttctcggcc ttcccgctgc cgggcctgct gttcctgtcg gccgtcaaac
     2401 tgctcaagac cagcatctat ctgctgatgg cggcggtgtt cgcccaggcc atcctgtcct
     2461 ggctgaatcc ccataccgca gcagccccgt tgctggatgc cgtcacccgc cgcttcctgc
     2521 aaccgctgcg ccgcatcgtg ccgatgctcg gcaacatcga cctgtccggt ctgctgctgt
     2581 tcatcatctg ccagctcatg gtgatcgtgc cgatcaccat gctggaacgc atcgcggcca
     2641 gattgcttta aatgaccgag tggtatcgcc gtcagggcga ggtactcacg ctgacgctgc
     2701 atatccagcc gggcgcgaag cgtagcgaac tcgccggact gcacggcgag gcgctcaaaa
     2761 tccggcttgc cgccccgccg gtagagggtc gcgccaacga agcgctgctg aagttcatcg
     2821 cgaaattatt cgaggtgccg gtgcggcagg ttgagctcaa acagggcggg cagtcacgcc
     2881 acaaggtcat cgcgattacc ggcagcaatg tcgacccgtt aagcctgctg gatagctgat
     2941 acggttcaac ccgccggcag cgggctgttt cacccattgc ccagaaacag ctcaagatgc
     3001 gacaggaagg cgcgcgtctt gaccggcagg tagcggcgca tcggcatcac cgcccaggcc
     3061 ggaacggcag gcaacgacca ttccggcaac actctgacca gccgcttgaa ccgcacctcc
     3121 tccgccgcca accggcacgg caccgccccg ataccggcgc cgtccagcaa taactgctgg
     3181 atcatgtcgg gcgaattcag cgtcagccgt ccgggcggaa ccccttccca gacctccttg
     3241 ccgcgcacca gcttccaggg aattgccgcc cctcgcgcag acagcaggcg aaccgcagca
     3301 tggtgctgca gatcgtccgg atgccggggt gccggatgca gcgccagata gatcggactg
     3361 gcatacagcc ccaagagatg ctcgtcaatt ttccgcgcaa ccagcgtcgc atcgttttcc
     3421 agcgttccca tgcgtatcgc cagatcgaaa cgttcgccga tcagatcgac gcgccgcgag
     3481 gacaggtcga gatccagctg gatctcggga taggtctcga taaaggtggc gatcgcacgg
     3541 gagaaatatt gcctcgcgta atcgcccggc atggagacgc gcaaatgccc gcgcggccgt
     3601 gcctcctgac tgcgcacgaa gtcctgcgcg gtggcgacct cttcggcaat gcgacgacaa
     3661 tgatcgagaa attcctgacc aaattcggtc agcgtgaggc gacgggtcgt gcgtatcagc
     3721 aagcgctgcc cgagcgccgt ttcaagattg gtcaagcggc gggaaaccgt ggctttcggc
     3781 atcgccagac tctccgccgc gcgcaccaga ctcccctgtt caacgatggc ggcgaacagg
     3841 atgtaatcat ctgcagacag gcttctttgt tccatttgtg gaacagtcta tcccagtacc
     3901 ctgactttat ctattcccgc agcggcttat catgcagtca ctttctccaa ctaaggagtc
     3961 ttccatgaac accgccacaa caaccaccct gaatcactcg cgcagcatcg agcgcatcgt
     4021 cgaaggcgtc gccacctcgg acggcgcagg cgtgaaactg actcgcgtac tcaccggcaa
     4081 gctgcaacgc cgtctcgacc ccttcctgat gctggacgca ttcggcagcg acgatcccga
     4141 cgactacatc gccggcttcc ccgatcatcc gcatcgcggc ttcgaaacgg tgacttatat
     4201 gctgcgcggc cgcatgcgcc atcgcgacag cgccggtcac gagggactgc tgggcgacgg
     4261 cggcgtgcag tggatggttg ccgggcgcgg ggtggttcac tcggagatcc ccgaacagga
     4321 agacggcgtg atggaaggct ttcagctttg gctcaacctg cccgcacaga gcaagatgac
     4381 cgatccgtgg tatagagact atgccagtgt agcgattccc gaatacgtca cccaggaagg
     4441 cgtcaccgtg cgggtgatcg cgggcagcag caacggtgtg gcgggtgcgg tttctcgcga
     4501 aatcaccgag ccgctgtatc tcgacatcca tcttcctgcg ggaaccggat tctcgaccgc
     4561 catccccgcc tcgcacaacg cgttcatcta cgtctatcgc ggcgcggtaa aggtcggggg
     4621 agtgcaggtg gattcgcaac gcatgggcat tctgagcaac agtgccgacg gcttgtcgtt
     4681 atcggcatcg ggagatgcgc gcctgattct ggtggcaggc aaaccgctca acgagcccat
     4741 cgtgcagtac gggccgttcg tgatgaactc gcaggaagag atacgtcagg cactggatga
     4801 ttttagagcc gggcatttcg cgtaaatgag cattcgcagg ctgggcacgt tgttctgcct
     4861 gccctgacaa atttttgagg aggcaattta tgagcacccc catcacccgc tatagcaaca
     4921 ccgccatcgc cttgcactgg ctgatggcac tgctgatctt cgcggcgttt ccgctggggc
     4981 tgtatatgca cgacttgcct ttatcgccca ccaagttgca gctctacagc tatcacaaat
     5041 ggataggcat cacggtgctg ctgctcgctg tgttcagggt gatgtggcgc gcgacgcaca
     5101 cgccgcccgc gctgcccgac agcttgccgc gttggcagca actcgccagc catgccacgc
     5161 accagttttt gtatgtattg atctttgcgg taccgctgtc cggctggctg atgagctcgg
     5221 ccaagggctt caagaccttg tggttcggcg tgttgccgct gcccgatctg gtcggcaagg
     5281 ataaggcgct gggcgagttg ctcggcaacc tgcatgcgag cctgaattac ctgttgctgg
     5341 cgatggtagt gctgcatgtc gcagccgcat tgaagcatcg tttgatcgac cgtgatgacg
     5401 tgatgaaccg catgttgcct acaggagaac gaaaatgaac attctgaaat acctcgcggt
     5461 gtgcgggctg acgctggcat gcagtcaagt ctccgcacag gagttcaagc aagtgctggc
     5521 caacgagagt tcggtgacgt tcggctacaa gcaaatgggc gtaccgatgg acggcaagtt
     5581 caacaggttt gccgcgcagc ttgcgttcga tccgtccagg gtcgccaatg cgcaagcgcg
     5641 catcgacatc gatgtggcca gcatcgacac cggatcgacc gaggccaacg atgaagtggt
     5701 gggcaagttg tggtttaacg ccaagactta tccggctgcg agctttacct ctgccggatt
     5761 gaaagccttg ggcggcaacc gttatgaggc tagaggcaaa ctcagcatca agggccggac
     5821 actggatata gttgcacccg tcacttttca ggccacaggc agtcgcggtt cattcgacgg
     5881 cagcttcacc atcaagcggc tcgactacgc catcggcgag ggcgaatgga ccgatgtcgg
     5941 caccgtcgca aatgaaattc aagtcaagtt tcacctcgtc gtcaatgcca caccgagcaa
     6001 gaagtagttt caaatccaac caccaccaag gagaacacca tgaaaaaact agctaccctg
     6061 acgctcgcat ccctgctgtc ttccgctgct tatgccgcac cggaaactta tgtgatcgac
     6121 ccgaatcaca gc
//
LOCUS       DASUIV010000159         4595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_781213, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000159 DASUIV010000000
VERSION     DASUIV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781213"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFW59_05960"
     CDS             complement(<1..371)
                     /locus_tag="VFW59_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX5363792.1"
                     /translation="MSPSNSLSYRDAGVDIDAGDRLVENIKPYAKRTMRPEVLSGIGG
                     FGGLVEISKKYREPVLVSGTDGVGTKLKLAFELNRHDTVGIDLVGMSVNDILVQGAEP
                     LFFLDYFACGKLDVDAATEVI"
     gene            449..1507
                     /locus_tag="VFW59_05965"
     CDS             449..1507
                     /locus_tag="VFW59_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5363793.1"
                     /translation="MTLSRFAAVQWLSFVAVAGLLLYLLGPILTPFVAAAILAYICNP
                     LVRRLCGWKLSRGFAVVLVMAGLLLLFALSLLIMLPLLQKEVGLFVTRFPDWLEAVRL
                     NLLPQLQQRFGIVLEWDSQALKNLLLSHWQSAGGIASKLLPWLGSSGGALLALLANLL
                     LIPLAMFYLLRDWNGIIAHLDNLVPRHWHAKVHEIATEVDRVLAEFLRGQISVMLLMS
                     VFYVLLLWLVGLEFALPIGLVAGMLVFVPYLGMITGLALATLAATMQFTSFGGVLMVW
                     AVFGTGQLIEGMLVTPWLVGNRVGLHPLAVVFALMAFGQLFGFFGVLLALPMAAIFLV
                     ALRYAKAIYLNSSMYRKP"
     gene            1504..2175
                     /gene="hda"
                     /locus_tag="VFW59_05970"
     CDS             1504..2175
                     /gene="hda"
                     /locus_tag="VFW59_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEX5363794.1"
                     /translation="MSQLLLDIVHGSRPTLENFVAGRNLELLSALRHALAGGSERSFY
                     LWGESGSGKSHLLKSCISAAREAKRSTAYACGSVPLQSADRVADVVAVDDVEQLDDAA
                     QIALFDLYNRMREGGGLLLVSGTASPLHLNLRDDLRTRLGWGLVYQLHGLSDEEKAQA
                     LMQHAQSRGFALPPEVTQYLLRHGRRDLPSLMAALDGLDAHSLRLHRAPSVPLLKEVL
                     QQELK"
     gene            2172..2834
                     /locus_tag="VFW59_05975"
     CDS             2172..2834
                     /locus_tag="VFW59_05975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5363795.1"
                     /translation="MKLALFDLDNTLLNGDSDFEWSQFLIRIGVLDRELFEAKNLAFY
                     EHYKAGTLDINEFLDFQLKPLSRHARKTLDAWHEQFMQESVLPMITRPSRELVAKHQA
                     AGDICVIITATNSFVTAPIAREFGIDHLIATEPEHRDGEFTGRVAGVPSFREGKITRL
                     DSWLSERGWTLDSFADSVFYSDSLNDLPLLRKVKRPVAANPDDTLRAHAEQHGWPIVN
                     LR"
     gene            2843..3067
                     /locus_tag="VFW59_05980"
     CDS             2843..3067
                     /locus_tag="VFW59_05980"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5363796.1"
                     /translation="MSPLVLYGTSGCHLCAAARVALERAGVSAVSVDIAEEAELLERY
                     GMRIPVLRRIDDGAELGWPFDRDAVERFVA"
     gene            complement(3131..>4595)
                     /locus_tag="VFW59_05985"
     CDS             complement(3131..>4595)
                     /locus_tag="VFW59_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5363797.1"
                     /translation="GMAHLALQSESLPKIKNHLEKIKFSGEHLLGIIDEILDFSKLDA
                     KKVKLDNIAFKLNGMIENVIGMTAEKAHAKGIELTVDVAPSLDIELHGDPLRLSQVLI
                     NFIDNAIKFTKKGRVTLRVHQLDEDESSCTLRFEVQDTGIGISGMDKCKLFQPFQQAD
                     ASITREYGGTGLGLAISKQLVEMMNDGKIGVQSSPGEGSAFWFSARFNKSFHPIGKSD
                     ETYTEQPSGMPPSFSGARILIAENHPLNQEVIIEFLENADAIACVAQNGAEAIDLLTK
                     EHFDCVFMDVQMPVMDGYQATRLIRADPALANIPIIAMTANVGQEDRDRCLAAGMNDF
                     ISKPFKPQKLYAMLAKWLPKHSAPHANGQPAEAVHEELKSDPAVIDFSVLAELVGTEK
                     QKMREFARRFVKLMRNDMEVLMEALELEDIESLQKWGHNNKSTARMIGAIGFSELCQT
                     LEWHSKNDGNIEQARQIASKLHLLLNRINEQISHVLT"
BASE COUNT          780 a         1320 c         1444 g         1051 t
ORIGIN      
        1 ttgatgactt cggtggcggc atccacgtcg agtttgccgc aggcgaagta gtcgaggaag
       61 aacagcggct ccgcgccctg caccagaatg tcgttcacac tcatgccgac cagatcgatg
      121 ccgacggtgt cgtggcgatt cagctcgaag gcgagcttca gcttggtgcc gacgccgtcg
      181 gtacctgata ccagcacggg ttcccggtat tttttcgata tttccaccag gccgccgaag
      241 ccgccgatgc cggacagcac ttcggggcgc atggtgcgct tggcgtacgg tttgatgttc
      301 tcgacaagac ggtcgcccgc atcgatatcg acgccggcat cgcggtagga cagggaatta
      361 gatgggctca agttgagttc tcgctttcta aaaggtaaag tgcagctcgt ttttatatag
      421 ttcgttttcg ccgcgaattt tacccgaaat gaccttatcc cgctttgctg ccgtgcagtg
      481 gctgtcgttc gtcgcagtcg ccgggctgtt gctctacctg ctcgggccta tcctgacgcc
      541 ctttgtggcg gcggccatcc ttgcctatat ctgcaatccg ctggtgcggc ggttgtgcgg
      601 ctggaagctg tcgcgcggtt tcgcggtggt gctggtgatg gccggattgc tgctgctgtt
      661 cgccctgtcg ctgctgatca tgctgccgct gctgcagaag gaggtggggc tgttcgtcac
      721 gcgcttcccg gactggctcg aggcggtgcg cctgaatctg ctgccgcagt tgcagcaacg
      781 cttcggcatc gttctggaat gggacagtca ggcattgaag aatctgctgc tcagccactg
      841 gcagagcgcg ggcgggatcg catcaaaact gttgccctgg ctgggcagca gtggcggcgc
      901 cttgctcgct ctgctggcca acctgctgct gattccgctg gcgatgttct acctgctgcg
      961 cgactggaac gggatcatcg cgcatctcga caacctcgtg ccgcgccact ggcatgcgaa
     1021 ggtgcacgag atcgcaactg aagtggaccg tgtccttgcc gaattcctgc gcggccagat
     1081 atcggtgatg ctgctgatga gtgtgtttta cgtgctgttg ctgtggctgg tcgggctgga
     1141 gttcgcgctg cctatcggtc tggtcgccgg gatgctggtg ttcgtgccct atctcggcat
     1201 gatcaccgga ctggcgctgg caacgctggc ggcaacgatg cagttcacct cgttcggcgg
     1261 tgtgctgatg gtgtgggcgg tgttcggcac ggggcagctg atcgagggca tgctggtcac
     1321 gccctggctg gtcggtaacc gggtcgggct gcatccgctg gcggtcgtgt tcgcactgat
     1381 ggcgttcggc cagttgttcg gtttcttcgg tgtgctgctg gccctgccga tggccgcgat
     1441 cttcctggtg gccctgcgct atgcgaaggc catctatctg aacagctcga tgtatcgcaa
     1501 accatgagcc agttgctact cgatatcgtt cacggcagcc gcccgacgct ggagaatttt
     1561 gttgccggcc gcaacctcga attgctctcg gcgctgcgtc atgcgctggc gggcggcagc
     1621 gaacgcagct tctatctgtg gggcgagagc ggcagcggca agagtcatct gctcaaatcc
     1681 tgcatcagcg ccgcacgcga ggccaagcgc agcacggcct atgcctgcgg cagcgtgccg
     1741 ctgcagagtg cagaccgcgt ggcggacgtg gtggcggtgg acgatgtcga acaactcgac
     1801 gatgccgcgc agatcgcgct gttcgacctg tataaccgca tgcgcgaggg cggaggtctg
     1861 ctgctggtga gcggcacggc atcgcccctg cacttgaatc tgcgcgacga cctgcgcacg
     1921 cgtctgggct gggggctggt gtatcaactg cacggtctga gcgacgaaga gaaggcgcag
     1981 gcgctgatgc agcacgccca atcgcgcggc ttcgccttgc cgccggaagt cacgcaatac
     2041 ttgctgcgcc acgggcggcg cgacctgccc agcctgatgg cggcgctgga cggactggac
     2101 gcgcactcgc tgcgcctgca tcgcgcaccc tcggtaccct tgctcaagga agtcttacaa
     2161 caggagttga agtgaaacta gccttattcg atctggacaa tactctgctc aacggcgaca
     2221 gcgatttcga gtggtcgcaa tttctgattc gcatcggcgt gctcgaccgc gagctgttcg
     2281 aggcgaagaa cctcgcgttc tatgaacact acaaggcggg gacgctggac atcaacgaat
     2341 tcctcgactt ccagctcaag ccgctgtcgc gccatgcgcg caagacgctg gacgcgtggc
     2401 acgagcagtt catgcaggag agcgtgctgc cgatgatcac ccggccgtcg cgcgagctgg
     2461 tggcgaagca ccaggcggcc ggcgatatat gcgtcatcat caccgccacc aacagcttcg
     2521 tcaccgcgcc gatcgcgcgc gagttcggca tcgaccatct gatcgccacc gaaccggagc
     2581 acagggacgg cgaattcacc ggccgcgtcg ccggtgtgcc gtcgttccgc gaaggcaaga
     2641 tcacccggct ggacagctgg ctgagtgagc gcggctggac gctggacagc ttcgccgaca
     2701 gcgtgttcta cagcgactcg ctcaacgatc tgccgctgct gcgcaaggtg aagcgtccgg
     2761 tcgccgccaa cccggacgac acgctgcgcg cgcatgccga gcagcacggc tggcccatcg
     2821 tcaacctgcg ctaggttttc gcatcagccc gctcgtccta tacggcacct ccggttgcca
     2881 cctgtgcgct gcggcgcgag tcgcgctgga gcgtgcgggc gtatccgcag tgtctgtcga
     2941 tatcgccgaa gaggccgaac tgctggagag atacggcatg cgcatcccgg tgttgcggcg
     3001 tatcgatgac ggcgcagagc tcggctggcc gttcgatcgg gatgccgtgg agcgtttcgt
     3061 tgcttgatgc agcagccagg aagtagtgct ctatttcggc cagaggacag gcaggggttg
     3121 gttgtaacgc tcaggtcagg acgtgcgaga tttgttcatt gatgcggttg agcaacaggt
     3181 gcagcttgct ggcgatctgt ctggcctgtt cgatgttgcc atcgtttttg ctgtgccatt
     3241 ccagcgtctg acacagctcc gagaatccta tcgcgcctat cattctggct gttgacttgt
     3301 tgttgtgccc ccatttttgg aggctctcga tgtcttccag ttcaagggcc tccatcagca
     3361 cttccatgtc gttccgcatc aatttaacga acctgcgggc aaattcgcgc atcttttgtt
     3421 tttcggtacc gacgagttcg gccagcactg aaaaatcgat caccgccgga tcgctcttca
     3481 attcctcatg cacggcttct gcaggctgtc cgttagcgtg aggcgccgag tgtttgggga
     3541 gccacttggc cagcatggcg tagagctttt gcggtttgaa cggcttgctg atgaagtcat
     3601 tcatgcccgc tgccaggcag cgatcccggt cttcttggcc gacgttggcg gtcatggcga
     3661 tgatcgggat gttggccaat gccgggtcgg cccggatcag ccgagtcgcc tgatagccgt
     3721 ccatcaccgg catctggaca tccataaaga cgcagtcgaa gtgctccttg gtcaacaggt
     3781 cgatcgcttc tgcaccgttc tgggcgacgc aggcgatggc atcggcattt tcgagaaact
     3841 cgatgatcac ttcctgattg agcggatggt tttcggcaat cagaatgcgc gcgccgctga
     3901 acgagggggg catgcctgat ggctgttcgg tataggtctc atcgctcttt ccgatggggt
     3961 ggaatgactt gttaaagcgt gcgctgaacc agaaggcgct gccttcgccg ggcgaacttt
     4021 gcacgccgat cttgccgtca ttcatcatct ccaccagttg cttgctgatg gcaaggccca
     4081 ggccggtgcc gccgtattcg cgcgtaatgg aagcatcggc ctgctggaac ggctggaaca
     4141 gtttgcattt gtccatacca ctgatgccta tgccggtgtc ttgcacttca aacctgaggg
     4201 tgcagctgct ttcatcctcg tcgagctggt ggacgcgcag ggtgacccga ccttttttcg
     4261 tgaatttgat ggcattgtcg atgaagttga tcagcacctg actgagtctg agcgggtcgc
     4321 cgtgcagctc aatatcgagt gatggggcga catcgaccgt cagttcaatg ccttttgcat
     4381 gagccttttc tgcggtcatg ccgatgacgt tttcgatcat gccgttcagt ttgaaggcga
     4441 tgttatcgag cttgaccttt ttcgcatcca gcttggagaa atctagaatc tcgtcgatga
     4501 ttccgagcag atgttccccg gagaatttta ttttctcgag atgatttttg atctttggga
     4561 ggctctccga ttgaagtgcc agatgtgcca tgccg
//
LOCUS       DASUIV010000160         6504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1061163, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000160 DASUIV010000000
VERSION     DASUIV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061163"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /gene="aceE"
                     /locus_tag="VFW59_05990"
     CDS             <1..1944
                     /gene="aceE"
                     /locus_tag="VFW59_05990"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX5363798.1"
                     /translation="AISMAGREKLDNLIFVINCNLQRLDGPVRGNGKIIQELEGVFRG
                     AGWNVIKVVWGGAWDRLLAKDKTGLLLKRMEEVVDGEYQTYKSKDGAYVRSHFFGKYP
                     ELLELVADMSDDEIWRLNRGGHDPRKVFAAYTAAVKHKGQPTVILAKTVKGYGMGSSG
                     EAQNPTHQQKKMDGESLRKFRDRFNIPVSDADLPNLPFVRPAEDSQEMKYLRDRVAEM
                     GSVPSRMPEAQPLPMPASEAFDAMLKGTDGREISTTMAFVRLLGALVKDKHIGKQVVP
                     IVPDESRTFGMEGMFRQLGIFSQVGQLYTPQDADQLMYYKEDKTGQILQEGINEAGGM
                     ADWIAASTAYANHGVAMIPFYIYYSMFGFQRIGDLAWAAGDLRARGFMVGGTAGRTTL
                     NGEGLQHQDGHSHLMAATIPNCVSYDPVFSYELAVIVREGMRRMYVEQEDVFYYLTVM
                     NENYEHPAMPKGAEVGIVKGMYLFREGKAKANAPKVQLLGSGTIMREAIAAADLLEKD
                     FGIAADLWSVTSFNELRRDGLDCERWNMLHPEAKPRASFVEQCLAKRSGPVIAATDYI
                     KSYADQIRPYVTARYKVLGTDGFGRSDTRKKLRQFFEVDRHYIVVAALKALADEGTIE
                     AVQVSRALKLYSINPDKPNPTTV"
     gene            1963..3222
                     /gene="aceF"
                     /locus_tag="VFW59_05995"
     CDS             1963..3222
                     /gene="aceF"
                     /locus_tag="VFW59_05995"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEX5363799.1"
                     /translation="MAEIKQVLVPDIGSYKDVGVIEVMVKVGDSIKAEDNLLTLETDK
                     AAMDVPSPYDGVVKEVKVKVGDKVSEGGVILLLEVSGAAAAATAAPAAAPAATPAPVA
                     AKAAPAVTTSVQPVQAAGGKGHASPAIRRFARELGVDIAQVKGSGEKSRVTKEDVQNF
                     VKQSLAQPRGAAGGLAVLDMPVVDFAKFGAIETKPLSRIKKISGANLHRNWVTIPHVT
                     QFDEADISEMEAFRKQLGAEYAKQNIKITPLAFLVKAAAAALQQFPDFNASLDAGGEN
                     LVLKQYCHIGVAVDTPDGLMVPVLRDVDKKGLVQIAKELGELGARAREKKITAADMQG
                     GCFTISSLGGIGGTAFTPIINAPEVAILGVSRSSMKPVYQDGEFVARLMLPLSLSYDH
                     RVIDGAAAARFTTYLGQVLSDIRRLAL"
     gene            3226..3885
                     /gene="nadD"
                     /locus_tag="VFW59_06000"
     CDS             3226..3885
                     /gene="nadD"
                     /locus_tag="VFW59_06000"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX5363800.1"
                     /translation="MSNRPVGILGGTFDPIHFGHLRLAEEMLELAGLEKIRFIPSGVP
                     PHRDSPQVSPQHRSAMVKLAIADQPAFMLDEREVARTTPCYTVDTLRELRAELGSEKP
                     LCLLMGGDAFLQLHTWHEWEQLFELAHIVVGHRPGYALDERIGSTTATLRNHYHRRLC
                     SVADIAQSPCGGVAELAMPKLEISATDIRRRVAEGRTIRYLLPAAVADYINQHHLYTS
                     C"
     gene            3879..4244
                     /gene="rsfS"
                     /locus_tag="VFW59_06005"
     CDS             3879..4244
                     /gene="rsfS"
                     /locus_tag="VFW59_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5363801.1"
                     /translation="MLTIEEKTKAVVDALEDIKADDIVVVDTSKLSPLFDRMIIASAK
                     STRQTKAIADNVVVKLKEQGEQVFGREGGDSGEWILVDLGEVLVHIMQPAVRAYYNLE
                     ELWTKGQDFRPKLAAKKPE"
     gene            4278..4748
                     /gene="rlmH"
                     /locus_tag="VFW59_06010"
     CDS             4278..4748
                     /gene="rlmH"
                     /locus_tag="VFW59_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX5363802.1"
                     /translation="MKLIIVSVGHKMPDWIATGFNEYAKRMPREAKIELVEIKPEPRT
                     TGKTTPQIMEAEAQRILGALPQSCLRIALDEHGAQPTTKKLAAQMQDWMREGRDVAFI
                     IGGADGLHESVKQAAQHLMALSSLTLPHAMVRVLLAEQLYRAHSLMHNHPYHRE"
     gene            4872..5492
                     /locus_tag="VFW59_06015"
     CDS             4872..5492
                     /locus_tag="VFW59_06015"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEX5363803.1"
                     /translation="MSNIQSRIYLASQSPRRRELLKQVGINFEMLLLRSNPGRNQDVD
                     ETPHPDETPDVYVRRVCSVKAEAGCLAVRQRNLPSFPVLAADTTVTLDGKIFGKPDHA
                     EHAAEMLRALSGREHQVLSAVAVAMGEHTEVAVSVSTVRFAVLDEERIRRYLLTREYT
                     DKAGAYAIQGHAGAFIEHIAGSYSGVMGLPLFETIELLRHFDYPTI"
     gene            5500..>6504
                     /locus_tag="VFW59_06020"
     CDS             5500..>6504
                     /locus_tag="VFW59_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5363804.1"
                     /translation="MSEEILINVTPQETRVAVMQLGVVQDLHIERSSSRGIVSNVYMG
                     RVKRVLPGMQSAFIDIGLERSAFLHVADIWGNGSEGSEARPIEKILQDGQTLMVQVIK
                     EPIGSKGARLSTQISIAGRLLVYLPQETHIGVSQRIESVEQRDALRARLQALLPEGHQ
                     GGYIIRTVVEAAIEPDFAADISYLDKLWNNLKTASQTAGAPQLLYRELDISLRVLRDF
                     VTLETTRILVDSRSTHQKMLEFAQAYNPLATERLEHYPGVRPLFDLYSVEEEIERALA
                     KRVDLKSGGYLIIDQTEAMTTVDVNTGGFVGLRNFDDTIFKTNLEATQVIARQLRLRN
                     L"
BASE COUNT         1338 a         1902 c         2086 g         1178 t
ORIGIN      
        1 gcgatttcga tggccggacg cgagaagctg gacaacctga tcttcgtgat caactgcaac
       61 ctgcaacgtc tcgacggccc ggtgcgcggt aacggcaaga tcatccagga gctggaaggt
      121 gtgttccgtg gcgcaggctg gaacgtcatc aaggtggtct ggggtggcgc atgggatcgc
      181 ctgctggcca aggacaagac tggcctgctg ctcaagcgca tggaagaagt ggtcgatggc
      241 gaataccaga cctacaaatc caaggatggc gcctatgtgc gatcacactt cttcggcaaa
      301 tatcccgagc tgctggagct ggttgccgac atgtcggacg acgagatctg gcgtctgaac
      361 cgtggcggtc acgacccgcg caaggtgttc gctgcctaca ccgcagcggt aaagcacaag
      421 ggccagccga ccgtgattct tgccaagact gtgaagggtt acggcatggg ttcgtccggc
      481 gaggcgcaga atccgaccca tcagcagaag aagatggacg gcgagtcgct gcgcaaattc
      541 cgcgaccgtt tcaatattcc ggtgagcgat gccgatctgc ctaacctgcc tttcgtgcgt
      601 cccgccgaag acagtcagga aatgaaatat ctgcgcgacc gcgttgcgga aatgggctcg
      661 gtgccatcgc gcatgccgga agctcagcca ttgccgatgc cggccagcga ggcgttcgac
      721 gcgatgctga aaggtaccga cggccgcgag atctccacca ccatggcgtt cgtgcgcctg
      781 ctgggtgcgc tggtcaagga caagcatatc ggcaagcagg tcgtgcctat cgtgccggac
      841 gagtcgcgca ccttcggcat ggaaggcatg ttccgccagc tcggcatctt ctcgcaggtg
      901 ggccagttgt acaccccgca ggacgccgat cagctgatgt attacaagga agacaagacc
      961 ggccagattc tgcaggaagg catcaacgag gcgggcggca tggccgactg gatcgcggct
     1021 tcgaccgcct acgccaacca cggcgtggcg atgatcccgt tctacatcta ctactctatg
     1081 ttcggcttcc agcgcatcgg cgatctggcc tgggcggcgg gcgacctgcg cgcgcgcggc
     1141 ttcatggtcg gcggcacggc cggacgtacc acgctgaacg gcgaaggtct gcagcatcag
     1201 gacggccaca gccatctgat ggcggcgacc atccccaact gcgtcagcta cgacccggtg
     1261 ttctcctacg agctcgcggt gattgtgcgc gagggtatgc gccgcatgta tgtggagcag
     1321 gaagacgtgt tctattacct gacggtgatg aacgagaact acgaacaccc ggccatgccg
     1381 aagggcgcgg aagtcggcat cgtcaagggt atgtatctgt tccgcgaagg caaggccaaa
     1441 gccaatgcgc ccaaggtgca gctgctgggc agcggtacca tcatgcgcga ggcgatcgcc
     1501 gccgcagatc tgctggagaa agacttcggc atagccgccg atctgtggag cgtgaccagc
     1561 ttcaacgaac tgcgtcgcga tggtctggac tgcgagcgct ggaacatgct gcatccggaa
     1621 gccaagccgc gcgcaagctt cgtcgagcag tgtctggcca agcgcagcgg cccggtgatc
     1681 gcggcgaccg actacatcaa gagctacgcc gatcagatcc gcccctatgt gaccgcgcgt
     1741 tacaaggtgc tgggaacgga cggtttcggt cgttcggata cgcgcaagaa gctgcgtcag
     1801 ttcttcgagg tggacaggca ctacatcgtc gtggcggcac tcaaggcgct ggccgacgaa
     1861 ggcacgatag aggcggttca ggtgagccgc gcgctcaagc tgtacagcat caatccggac
     1921 aaacctaatc cgaccacggt gtaagggctc agggagaata cagtggcaga aatcaaacaa
     1981 gtactggtgc cggatatcgg cagctacaag gacgtgggcg tcatcgaggt gatggtcaag
     2041 gtgggcgaca gcatcaaggc ggaagacaat ctgctgacgc tggagaccga caaggcggcg
     2101 atggatgtgc cgtcgcctta tgacggcgtg gtcaaggaag tgaaggtcaa ggtaggcgac
     2161 aaggtgtcgg aaggcggcgt gattctgctg ctggaagtga gcggcgctgc cgcagctgca
     2221 acggctgcgc ccgccgcagc gcctgcagca acacccgcac cggttgcagc caaggcggca
     2281 cctgccgtca cgaccagcgt gcagccggtt caggcagcgg gtggcaaggg acatgcgagt
     2341 ccggcgatcc gccgtttcgc gcgcgagctg ggcgtggaca tcgcgcaggt caaaggctcg
     2401 ggcgagaaga gtcgcgtcac caaagaggac gtgcagaact tcgtcaagca gtcgctggcg
     2461 caaccgcgcg gtgcagcggg cggtctggcg gtgctcgaca tgccggtggt ggacttcgcc
     2521 aagttcggcg ctatcgagac caagccgctg tcgcgcatca agaagatatc cggcgcgaat
     2581 ctgcaccgca actgggtgac gattccgcat gtcacgcaat tcgacgaggc cgatatcagc
     2641 gagatggagg cgttccgcaa gcagctgggt gcggagtacg ccaagcagaa tatcaagatc
     2701 acgccgctgg cgtttctggt caaggctgcg gccgccgcgc tgcagcagtt tcctgatttc
     2761 aatgcatctc tggatgccgg cggcgagaat ctggtactca agcagtactg ccatatcggc
     2821 gttgcggtgg atacgccgga cggtctgatg gtgccggtgc tgcgcgacgt ggacaagaag
     2881 gggctggtgc agatcgccaa ggaactgggc gagctcggtg cgcgtgcgcg cgagaagaag
     2941 atcactgcgg ccgacatgca gggcggttgc ttcacgatct ccagtctggg cggcatcggc
     3001 ggtacggcgt tcacgcctat catcaacgcg ccggaagtgg cgatcctggg cgtgtcgcgt
     3061 tccagcatga agccggtgta tcaggacggc gaattcgtcg cgcgtctgat gctgccgctg
     3121 tcgctgtcct acgaccatcg cgtgatcgac ggtgctgcgg cggcgcgctt caccacctat
     3181 ctcgggcagg tgttgtcgga catccgccgg cttgcgctgt aatcggtgag caacagaccg
     3241 gtcggtatcc tgggcggcac cttcgacccg attcatttcg ggcatctgag gttggccgag
     3301 gaaatgctgg aactggccgg tctggagaag atacgtttta ttcccagcgg ggtgccgcct
     3361 catcgcgact cgccgcaggt ttcgccgcaa catcgcagtg caatggtgaa actggccatc
     3421 gcggatcaac cggcgttcat gctggacgag cgcgaagtgg cgcgtaccac gccctgttat
     3481 accgtggata ccttgcgtga attgcgtgcg gaactgggca gcgaaaagcc gctgtgcctg
     3541 ctgatgggcg gtgacgcctt tctgcagctg catacctggc acgagtggga gcagctgttc
     3601 gaactggcgc atatcgtggt gggacaccgc cccggttatg cgctggacga acgcataggc
     3661 agcacgacgg cgaccttgcg caatcactat caccggcggc tgtgctcggt ggcagacatt
     3721 gcgcaaagtc cgtgcggcgg ggtcgccgaa ctggcgatgc ccaagctgga aatatcagcc
     3781 accgacatcc gtcgccgtgt ggccgaaggt cgcaccatcc gttatctgct gccagccgca
     3841 gtggcggact acattaatca acatcactta tacacatcat gctaactatt gaagaaaaaa
     3901 ccaaagccgt cgtcgacgcg ctcgaagaca tcaaggccga cgacatcgtc gtggtcgata
     3961 ccagcaagct cagcccgctg ttcgaccgca tgatcatcgc cagcgcgaaa tccacccgcc
     4021 agaccaaggc catcgccgac aacgtggtgg tcaagctcaa ggagcagggc gagcaggtgt
     4081 tcgggcgcga aggcggcgac agcggcgagt ggatcctggt ggatcttggc gaagtgctgg
     4141 tgcatatcat gcagccagcg gtacgcgcgt attacaacct cgaagagctc tggaccaagg
     4201 gtcaggattt ccgtcccaag ctggctgcca agaagccgga gtaggagcgc ggtgcgccgt
     4261 actcgcattg ccgacctatg aagctcatca tcgtctcggt cggtcacaag atgccggact
     4321 ggatcgcgac tggctttaac gaatatgcca aacgcatgcc gcgcgaggcg aagatcgaac
     4381 tggtagagat caagcccgag ccgcgcacca ctggcaagac caccccccag atcatggagg
     4441 ccgaggcgca gcgcattctc ggcgcgctgc cgcagagctg tctgcgcatc gcgctggacg
     4501 agcacggcgc gcaacccacc accaagaagc tcgccgcgca gatgcaggac tggatgcgcg
     4561 aggggcgcga tgtggcattc atcatcggcg gtgcggacgg tctgcacgag tcggtcaagc
     4621 aggcggcaca gcacctgatg gcgctgtcct cgctcacgct gccgcacgca atggtgcgcg
     4681 tgctgctcgc agaacagttg taccgggcac acagcctgat gcacaatcac ccctatcaca
     4741 gggagtagac atgcagagag tagaaagtag accagcgcta tgcgcttcag cgggaagcgg
     4801 gaaaatctcc ccttccttgt caccggctcc cgattcccca ctttccactt cccactcgcc
     4861 aatcttcagc catgagtaac atccagtcgc gtatctatct ggcttcgcag agtccgcgtc
     4921 gccgtgaatt gctcaagcag gtcggtatca atttcgagat gttgctgctg cgttccaacc
     4981 ccggccgcaa tcaggatgtg gacgaaacgc cccatcccga tgaaacgccg gatgtctatg
     5041 tgcgccgtgt gtgcagcgtg aaggcggagg ccggctgtct cgcagtcagg cagcgcaatc
     5101 tgccctcctt cccggtgctg gcggcggata cgaccgtgac cctggacggt aaaatcttcg
     5161 gcaagcccga tcatgccgaa catgccgccg aaatgttgcg cgcactttcc gggcgcgagc
     5221 atcaggtgct cagcgcggtc gccgtggcga tgggcgagca taccgaagtg gccgtgtccg
     5281 tttccacggt gcgctttgcg gtgctcgacg aggagcgtat ccgccgttac ctgctgacgc
     5341 gcgaatacac cgacaaggcg ggtgcctatg cgatacaggg gcatgccggc gcattcatcg
     5401 agcacatcgc cggcagctat tccggtgtga tgggcttgcc tttgttcgag accattgaac
     5461 tgctgcgcca cttcgattac cccaccatct aaatactcca tgagcgaaga aatactgatc
     5521 aacgtcaccc cgcaggagac gcgcgttgcg gtgatgcaac tcggtgtcgt gcaggatttg
     5581 cacatcgagc gcagcagcag tcgcggtatc gtcagtaacg tatacatggg cagggtcaag
     5641 cgcgtgctgc ccggcatgca atcggccttc atcgacatcg ggctggaacg ttcggcattt
     5701 ctgcatgttg ccgatatctg gggaaacggc agcgaaggtt ccgaggccag gccgatcgag
     5761 aagatcttgc aggacggtca gacgctgatg gtgcaagtca tcaaggagcc catcggcagc
     5821 aagggtgcgc gtctgtccac ccagatcagc atcgcaggaa ggctgctggt ttatctgccg
     5881 caggagaccc atatcggggt gtcccagcgt atcgagagcg tcgagcagcg cgacgccctg
     5941 cgcgccaggt tgcaggcgtt gttgccggag gggcatcagg gcggctacat catccgtacc
     6001 gtggtcgagg cggccatcga gcctgacttc gccgccgata tcagttatct ggacaagctg
     6061 tggaacaacc tgaagactgc ttcgcagacc gcaggcgcgc cgcaattgct gtatcgcgaa
     6121 ctggatatca gcctgcgtgt gctgcgcgat ttcgtcacgc tggagaccac gcgtattttg
     6181 gttgattcgc gcagcaccca ccagaagatg ctggagttcg cgcaggccta taacccgctt
     6241 gcgaccgagc ggctggaaca ttatccgggc gtgcgtcccc tgttcgatct gtacagcgtg
     6301 gaggaggaaa tcgagcgcgc gctggccaag cgggtcgatc tgaaatccgg cggttatctg
     6361 atcatcgacc agaccgaggc gatgaccacg gtcgacgtga ataccggcgg ttttgtcggg
     6421 ctgcgcaatt tcgacgacac catcttcaag accaatctcg aagccactca ggtcattgcg
     6481 cgccagctgc ggttgcgcaa tctc
//
LOCUS       DASUIV010000161         7447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_410616, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000161 DASUIV010000000
VERSION     DASUIV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410616"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFW59_06025"
     CDS             complement(<1..785)
                     /locus_tag="VFW59_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX5363805.1"
                     /translation="MKKPKKLRSRKAGMPPGSLVHIGEVKTAKPAISVLDFGPDSLSE
                     SKLPDTASLAAYTRQNTNLWGNIYGLRNPADLTAIGSTFQLHPLVLEDILNNDQRQKV
                     DAYDNYLYVVLHRYEITTAPLSLVQDQISLIIGPDFLLSFQERQSRTFEPVRQRLRSD
                     RGNIRSAGTDMLAYSLFDSVVDSYFAVVEQLDDYAEKLEIEILGHPGPAVLENIHQFK
                     RCVSQLRRNLYPLRELLGSLDREHAHFFAPEIQLYLRDVYDHTV"
     gene            complement(782..1549)
                     /locus_tag="VFW59_06030"
     CDS             complement(782..1549)
                     /locus_tag="VFW59_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEX5363806.1"
                     /translation="MKVADHERRFGGVARLYGPVAAERLKQASICVVGVGGVGSWTLE
                     ALARTGIGQLAAVDLDMVAESNTNRQIHALGDIYGKAKVDAMAERILAINPDCDFTCI
                     EDFVTPENVAEILRPHFDVVIDAIDQTRVKAAMIAHCRLQGTPIVVAGAAGGQIDPTQ
                     VRISDLAATVQDPLLAKVRALLRREYGFPRDTRKKFGVSAVFSTEALRYPTAPASCEA
                     SAGPAGLSCAGFGSSVCVTSVFGMAAAARAIDLLLTK"
     gene            1729..2697
                     /gene="ispB"
                     /locus_tag="VFW59_06035"
     CDS             1729..2697
                     /gene="ispB"
                     /locus_tag="VFW59_06035"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEX5363807.1"
                     /translation="MTVEQLYALIGPDMKAVDAVIRDRLYSDVVLVRQVAEYIIGGGG
                     KRMRPALVLLSAGAMGYQGRHHHDLAAVVEFIHTATLLHDDVVDESALRRGRDTANAM
                     FGNAASVLVGDFLYSRAFQMMVEVDEMRVMRVLSDATNVIAEGEVLQLMNCHDADVDE
                     QRYLQVIRYKTAKLFEAAAQLGAILGGATPEVEQALADYGMHLGTAFQLVDDVLDYSG
                     QEADTGKHLGDDLAEGKPTLPLIYVMQNGTPEQAACVRRAIEEGGRDDFPAVLAAIHA
                     SDALGHARRQAALEAERAREAILCLRDSNYREALIQLSAFAVERNY"
     gene            2716..2792
                     /locus_tag="VFW59_06040"
     tRNA            2716..2792
                     /locus_tag="VFW59_06040"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2750..2752,aa:Pro,seq:cgg)
     gene            2981..3211
                     /locus_tag="VFW59_06045"
     CDS             2981..3211
                     /locus_tag="VFW59_06045"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEX5363808.1"
                     /translation="MKYLLLIAFVAVIWWVLKNRAGRPAQRPSKAPPDPEMMVICAHC
                     GVHLPQSDSVVENDVYFCSEAHRQAGKASGRP"
     gene            3208..4818
                     /locus_tag="VFW59_06050"
     CDS             3208..4818
                     /locus_tag="VFW59_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5363809.1"
                     /translation="MTDWLSSTWEASWRPFLYFNLYRLLLGGLALLGALLPEGWIASL
                     HLSYSLIFLLSAVIYLFVVGAGLLAAIHWQNRFNFQLSLQVMTDVAAIGLLMYTGGGV
                     GSGIGALMLVSLATASLVGRGRLVLFYAALATLATLGGQVFGVFFGDFEPATIVQAGF
                     LSAGFFATAILARLLGQRLMVNEELARQRGISLENQSRISQRVIERMQDGVLVVDSAG
                     QVQRHNPMVEAMLGSAPAPGVHLADYCRPLAAALADWKVSSNEATTDFQVPAGGELRA
                     RFEQTTSSAGEVLVFLEDLGRIKERAQQLKLAALGRLTASIAHEIRNPLSAIGHAGEL
                     LREERRGAMQERLLKILADNVARLDRIVADILELGRRDRMQAESLDLGRFCSQFLESF
                     ENVQGVVPGVIVLEAADDLGIAFDRSHLDQIMWNLLSNAVRHARGAPGSVRLRACTGA
                     GKGLVELHVIDDGGGVPEAAKGQIFEPFFTTHHLGTGLGLFIARELCEANGATLELVS
                     DANHGHFIIVGRSDTCLLPEVSDARAAN"
     gene            4776..6125
                     /locus_tag="VFW59_06055"
     CDS             4776..6125
                     /locus_tag="VFW59_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5363810.1"
                     /translation="MSVARSERRPRGELSRVLVIDDEPDIRELIDLTLSRMGLAAACA
                     GSVAEARQALADGDFQLCLTDMRLPDGDGLEVVRFIAEHHAQTPVAVITAYGSTENAV
                     AALKAGAFDYLAKPVGLEQLRALVKSALRVPGGQHQDDPLQSLTGESAAMQQVRAMIE
                     KLARSQAPIYISGESGSGKELAARLIHVRSAQSVGPFVPVNCGAIPENLMESEFFGYR
                     KGAFTGAESDRDGFFQTASGGTLFLDEVADLPLAMQVKLLRAIQEKRVRKVGAVTEEP
                     VDVRIICATHRNLRDCVDRGTFRQDLYYRLNVIELRMPSLRERMEDIPSLVAAILKKL
                     CGDAIPQISAVALQALAAYPFPGNVRELENILERATALCSGAAIEADDLQLAPDEMGS
                     ENHGRGSETLDDYLNRLERQAILEALQKTEGNRTAAARLLGVTFRSLRYRLERLGIE"
     gene            6125..6691
                     /gene="ampD"
                     /locus_tag="VFW59_06060"
     CDS             6125..6691
                     /gene="ampD"
                     /locus_tag="VFW59_06060"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEX5363811.1"
                     /translation="MRWRGDGWLKGARWVFSPNQGGRPAGCEVSLIVLHNISLPPDNF
                     SGNWVERFFTNQLDPRAHPYFSTIAKLQVSAHFLVRRDGRVIQFVGCNRRAWHAGQST
                     WQGRENCNDYSVGIELEGSDSQPFTAKQYAELWPLIRKLRARYPIRALAGHCHVAPGR
                     KTDPGPYFDWSVVRIHCPGLALPAEVGG"
     gene            complement(6668..>7447)
                     /locus_tag="VFW59_06065"
     CDS             complement(6668..>7447)
                     /locus_tag="VFW59_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5363812.1"
                     /translation="NRHILRVYEGRPQNIPVGPETNFQPTPELLRQHWNARTAGLLVA
                     SPANPTGTLLTLPEIGALAEACREMQGHFLVDEIYHGLTYEIDAPTACAAGDDIWVIN
                     SFSKYFQMTGWRLGWMVIPEAYVRDIEKLAQNLVLCPSAPAQYGALAAFAPETIAILE
                     ARRAEFRARRDFLAPALESIGFRITSRPEGAFYLYCDCSALSNDSFALARDLLEKTGV
                     AATPGLDFGSNAPKKHIRFAYTTGIDRLAEAVNRLAAYFGR"
BASE COUNT         1384 a         2114 c         2358 g         1591 t
ORIGIN      
        1 acggtgtggt catatacatc gcgcagataa agctggattt ccggcgcgaa aaaatgcgca
       61 tgctcgcgat ccaggctgcc gagaagttcg cgtagcggat acagattgcg gcgcagttgg
      121 gatacacagc gtttgaactg atggatgttt tcgagaactg caggccctgg gtgtccgaga
      181 atctcgattt cgagcttctc ggcataatcg tccagttgtt cgaccaccgc gaaatagcta
      241 tcgaccaccg aatcgaacag cgaatacgca agcatgtcgg tgccggcgct gcggatattc
      301 ccccggtccg accgcaggcg ctggcgtacc ggctcgaaag tccgtgactg gcgctcctga
      361 aaactgagga gaaagtctgg cccgatgatc aggctgatct ggtcctgaac caggctaagc
      421 ggggcagtcg tgatctcgta gcggtgcaga acgacataga gatagttgtc gtaggcgtcc
      481 accttctgtc gctggtcatt gttgagaatg tcctcgagta ccagtgggtg tagctggaaa
      541 gtgctgccga tggcggtgag gtcggcagga ttccggagac catagatatt tccccacagg
      601 ttcgtgttct ggcgcgtgta ggcagccaga ctggcggtgt ccggaagttt actttcgctc
      661 agggaatccg ggccaaagtc gaggaccgat atcgccggct tggccgtctt gacctcgccg
      721 atatgaacca gcgagccggg cggcatgcca gccttgcgcg aacgcagctt tttcggcttt
      781 ttcacttggt cagcagcagg tcgatggcac gcgctgcagc agccatgccg aagaccgagg
      841 tgacacagac cgacgagccg aatcccgcgc agctcaaccc tgccggaccc gccgatgcct
      901 cgcagcttgc tggcgcagtt gggtaacgca gtgcctctgt cgagaatacg gcggataccc
      961 cgaatttttt cctggtgtcc cggggaaatc catattcgcg acgcaatagc gcacgaacct
     1021 tggccagtag cgggtcctgt acggttgccg cgagatcgct gatccttacc tgcgttggat
     1081 cgatctggcc gccggcggcg ccggcaacga caattggagt tccctggagg cggcaatggg
     1141 cgatcatcgc tgccttgaca cgtgtctggt cgatggcgtc gatcaccaca tcgaagtggg
     1201 gtcgcaggat ctcggccacg ttctcgggtg tgacgaaatc ctctatgcag gtgaagtcgc
     1261 agtcagggtt gatggccaga atacgttcag ccatcgcgtc gaccttggct ttgccgtaga
     1321 tgtccccgag ggcatgaatc tgccggttgg tgttggattc tgccaccata tcgaggtcga
     1381 ccgcagcaag ctgcccgatg cctgtcctgg cgagtgcttc cagcgtccag gagccaaccc
     1441 cgccgacgcc gacgacacag atgctggctt gcttcagccg ctcggccgcc acgggcccgt
     1501 acaggcgggc aaccccgccg aagcggcgct cgtgatcggc caccttcaaa tcgtgctgca
     1561 tcgtgccgcc aaagacaggg ccaatatgat tttttaccga tttgctcatg acctgccgag
     1621 tttagccgaa agcgccggcc gggaagaagg ccaaatcctg atttccgtgt cttgacggcc
     1681 cgtctgccca cgcctaagat tccgcgctta ccccaaagca ggtttaatgt gaccgtggaa
     1741 cagctttacg cccttatcgg gcctgacatg aaggcagtgg atgcggtcat ccgcgatcgt
     1801 ctttactcgg acgtcgtgct cgttcgccag gttgcagagt acatcatcgg tggtggcggc
     1861 aagcgcatgc gcccagcgct cgtgttgctg tcagcaggtg ccatgggtta tcagggcagg
     1921 catcaccatg atctggccgc ggtcgtcgag ttcattcata cggccaccct gttgcacgac
     1981 gatgtggtcg acgaatctgc gttgcgacgc ggtcgggata ccgccaatgc catgttcggc
     2041 aatgcggcca gcgtcctggt aggcgatttc ctgtattcac gtgccttcca gatgatggtc
     2101 gaggttgatg aaatgcgcgt catgcgcgtt ctctccgatg cgaccaatgt gattgccgaa
     2161 ggcgaagtgc tgcaactcat gaattgccac gatgccgatg tcgatgagca gcgctatctg
     2221 caggtcatcc gttacaagac ggccaagcta ttcgaggctg ctgcacaact tggtgccatt
     2281 ttgggtggtg ccacgcccga ggtcgaacag gcattggctg attacggcat gcatctggga
     2341 acagctttcc agctggtcga tgacgtgctc gactattcag gccaggaggc agataccggc
     2401 aagcatctcg gcgatgacct ggccgaaggc aagccgacgc tgccgctgat ttacgtaatg
     2461 cagaatggga cgccggaaca ggctgcctgt gtccgccggg cgatcgagga aggtggtcga
     2521 gatgatttcc ccgctgtcct ggcggctatc cacgccagtg atgcacttgg ccatgcccgc
     2581 cggcaggcgg cgcttgaggc cgagcgcgca agagaagcaa ttctctgtct gagggattca
     2641 aattatcggg aagctctgat acaattgtca gcctttgcag ttgagaggaa ttactagaag
     2701 cctctcataa agcatcggag tgtagctcag cctggtagag cactgcgttc gggacgcagg
     2761 ggtcgcaagt tcgaatcctg tcactccgac catttaaagc ccacgtaatt cattgaatta
     2821 cgtgggcttt tcatcttcta gagcccggcg gccatctctt gatgagcgaa taattgtggt
     2881 attctgaatt gtcgttcagc atactttccc tcagcttggg tcatcgcacc ccagcgtgct
     2941 tctgctgaag tttcagtcgg taaactcagg atcaacgaag atgaaatacc tgctgctcat
     3001 cgcttttgtc gctgtgatct ggtgggtatt gaaaaatcgc gctggccgac ccgcgcaacg
     3061 cccctcgaag gcaccccctg atccggaaat gatggtgatc tgcgcccatt gtggcgtgca
     3121 tttgccgcag agcgacagcg tcgtcgagaa cgacgtgtat ttctgctccg aagcgcatcg
     3181 gcaggccggt aaagcctctg gcaggccgtg accgactggc tgtcttcgac ctgggaggcc
     3241 agttggcgtc ccttcctgta cttcaatctc tatcgcctgc tccttggtgg ccttgcgttg
     3301 ctgggcgcat tgttgcccga gggatggatc gcctcgctgc acctctctta ttcgctgatt
     3361 ttcctgctgt cggcggtgat ttacctgttt gttgtcggtg ccggcctcct ggcagccatc
     3421 cattggcaga atcggttcaa ttttcagctt tcgttgcagg tgatgaccga tgtcgcggcg
     3481 atcggcctgc tgatgtacac gggtggcggt gttggcagcg gcatcggcgc cctgatgctg
     3541 gtttcactgg caaccgccag cctggtcggg cgtggccgcc tggtgctttt ttatgcagcg
     3601 ctggcgacgc tggcgacgtt gggggggcag gtcttcggtg tcttcttcgg cgatttcgag
     3661 ccggcgacca tcgtacaggc agggttcctg tcagccggat tctttgcaac ggccatcctg
     3721 gcgcgcctgc tgggtcagcg cctgatggtc aacgaggaac tggcccgcca gcgcggcatc
     3781 agcctggaga atcagagccg aatcagccag cgggtcatcg aacggatgca ggatggcgtt
     3841 ctggtggtgg atagtgctgg gcaggtgcag cggcacaacc cgatggtcga ggcgatgctc
     3901 ggttcggcac ctgctccagg ggttcatcta gctgactact gccggcccct ggccgctgcg
     3961 ctggcggact ggaaggtttc cagtaatgag gcgaccacgg attttcaggt gcctgccggt
     4021 ggtgaactgc gtgcccgctt tgaacagacg acaagcagcg caggggaggt tctcgtattt
     4081 ctcgaagatc tcggccgtat caaggagcgt gcgcaacagt tgaaattggc tgctttggga
     4141 cggttgactg ccagcattgc ccatgaaatc cgcaacccac tttccgccat cggccatgcc
     4201 ggcgagttgc tgcgggagga gcgtcgaggc gccatgcagg agcggctatt gaagattctg
     4261 gccgacaacg tggcacgcct cgaccgcatc gtcgcggata tcctcgaact cggccggcgc
     4321 gaccgcatgc aggccgagtc gctggatttg ggcagattct gctcgcagtt cctcgagagc
     4381 ttcgagaatg tccaaggggt ggtgccgggt gtcatcgttc tcgaagcggc ggacgatctc
     4441 gggatagcct ttgatcgtag ccacttggac cagataatgt ggaacctgct gtccaatgcg
     4501 gtacgccatg ccaggggtgc gcctggtagc gtgcgcttgc gggcttgcac aggtgccgga
     4561 aagggactgg tcgaactgca tgtcatcgac gatggcggcg gtgttccgga ggccgccaag
     4621 ggccagattt tcgagccatt cttcacgacc catcatctcg gcaccgggct gggtctcttt
     4681 atcgctcgcg aattgtgcga ggcgaacggt gccacgctgg aactggtgtc tgatgccaat
     4741 catggtcatt tcattattgt tgggagaagc gacacatgtc tgttgcccga agtgagcgac
     4801 gcccgcgcgg cgaattgagc cgtgtgctgg ttatcgatga tgagccggat atccgtgagc
     4861 tgattgatct cacgctgtcg cgcatgggct tggcggccgc ctgcgccggc agcgtcgccg
     4921 aggccaggca ggcgctggca gatggcgatt tccaactttg cctgaccgac atgcgcctgc
     4981 ccgatggcga tggcctcgag gtagttcgtt ttatcgccga gcatcatgcg cagacaccgg
     5041 tagcggtgat tacggcctac ggcagcaccg agaacgcggt ggcggcactc aaggctggcg
     5101 ccttcgacta tctggccaag ccggtggggc tcgaacagtt acgcgccctc gtcaagtcgg
     5161 cgctgcgcgt tcccggtggc cagcatcagg acgatccgct gcagtcgctg accggcgagt
     5221 cggcagcgat gcagcaggtt agggcgatga tcgagaagct ggcgcgcagc caagcgccca
     5281 tctacatctc cggtgagtcc gggagcggca aggaactcgc tgcgcgcctg attcatgtgc
     5341 gcagtgccca gtctgtcggc ccgttcgtcc cggtcaactg cggtgcgatc ccggaaaacc
     5401 tgatggaaag cgagttcttc ggctatcgca agggcgcctt caccggagcc gagagcgacc
     5461 gtgacggatt ttttcagacg gccagcggtg gcacgctctt tctggatgag gttgccgatt
     5521 tgccactggc catgcaggtc aagctgctgc gggcgattca ggaaaagcgg gtccgcaagg
     5581 tcggtgccgt gaccgaggag ccggtcgatg tgcgcatcat ttgcgcgacg caccggaatt
     5641 tgcgcgattg cgttgacagg ggaaccttcc gccaggattt gtattaccgc ctgaatgtca
     5701 tcgagttgcg gatgccgtcg ctgcgtgagc gaatggagga tattccttca ctggtcgccg
     5761 ccatcctgaa aaagctgtgc ggcgatgcca ttccgcaaat ttccgcggtt gccttgcagg
     5821 cgctggccgc ctatcctttc cccggcaatg tgcgcgagct ggaaaacatt ctggagcgtg
     5881 cgacggcact gtgctccggg gcagcaatcg aggcggatga cctgcaactg gcgccggatg
     5941 aaatggggag tgaaaatcac gggcggggca gcgagacgct cgatgactat ctcaatcgcc
     6001 tcgaacgcca ggccatactc gaagccttgc agaagacgga gggcaaccgg accgcggcag
     6061 cacgcctgct cggcgtcacc ttccgctcgc tgcgctaccg tctggaaagg ttgggcatcg
     6121 agtgatgcgc tggcggggcg atggctggct gaaaggcgcg cgctgggtgt tctctccgaa
     6181 ccaagggggc aggcccgccg gctgcgaggt ttcgctgatc gtgctacaca acatcagtct
     6241 gccgcccgat aatttttccg ggaattgggt tgagcgattt tttaccaacc aacttgaccc
     6301 acgggcccat ccgtattttt cgacgattgc taagctgcag gtttcagcgc attttttggt
     6361 gcgccgcgac ggtcgtgtga tccagtttgt tggctgcaac cggcgtgctt ggcatgccgg
     6421 tcagtcgact tggcagggca gggagaattg caatgactat tcggtcggta tcgaactcga
     6481 aggcagtgac agccagccgt ttacggcgaa gcagtacgcg gagctgtggc cgctaatcag
     6541 gaaacttcgt gcccgctatc cgatccgcgc gctggccggt cactgtcatg tggcgcccgg
     6601 ccgcaagacc gaccctgggc catatttcga ctggtccgtc gttcgcatcc actgcccggg
     6661 ccttgcgcta cctgccgaag taggcggcta accggttgac ggcttcggcc aggcggtcga
     6721 tgccggtggt gtaagcaaaa cggatgtgtt ttttcggcgc gttgctaccg aaatccagcc
     6781 caggtgtggc ggcaacgccg gttttttcaa ggaggtcgcg agccagcgcg aagctgtcat
     6841 tgctcagggc cgaacagtcg caatacaggt agaaggcgcc ttccgggcgg gaggtgatac
     6901 gaaagccgat gctttccagt gctggcgcca gaaagtcccg gcgtgccctg aactcggcgc
     6961 ggcgcgcttc gaggatggca atggtttccg gcgcaaacgc agccagcgca ccatactgcg
     7021 ccggcgccga tgggcaaaga accaggttct gggccagctt ctcgatatct cgcacatagg
     7081 cctccggaat gaccatccag cccagtcgcc agccagtcat ctggaaatat ttcgagaaac
     7141 tgttgatgac ccagatgtcg tcgccggcag cgcaggccgt cggggcatcg atttcatagg
     7201 tcaggccgtg atagatctcg tcgaccagga aatgtccctg catctcgcgg caggcctctg
     7261 ccagcgcccc gatctccggc agtgtcagca gcgtgccggt cggattggcc ggcgaggcga
     7321 cgagcaagcc ggcggtcctg gcgttccagt gctggcgcaa gagttccggt gtcggctgga
     7381 aattggtttc cggcccgacc gggatgttct gcggtcgacc ttcgtaaacc cgcaaaatat
     7441 ggcggtt
//
LOCUS       DASUIV010000162         6023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1340025, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000162 DASUIV010000000
VERSION     DASUIV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340025"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2644)
                     /gene="ligD"
                     /locus_tag="VFW59_06070"
     CDS             complement(65..2644)
                     /gene="ligD"
                     /locus_tag="VFW59_06070"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX5363813.1"
                     /translation="MATPSLDQYQHKRNFRRSPEPESTAPAVQTGHLYVMHKHAARRL
                     HFDLRMEQDGVLRSWALPKGAPLEIGEKRLAIEVENHPLEYGEFEGNIPKGEYGGGTV
                     MLWDVGLWQTDGRFDDKQIDFILTGAKLKGAWTLVRMRDWDVHGKNKQWLLIKRTDHP
                     QHDLHPDDLSVASGRSMAEIAANSEHHDAGARPPPPSAKDFPHARKAPPPATLPPQLA
                     TPGERAPTGQAWLHEIKFDGYRIVAHIEHGKARLMTRNGHDWTKRLHAQARQLEALPV
                     QQAILDGELVALSAGGATSFRELQEALSRKQTAQLVYQLFDLVYLDGYDLSALPLHVR
                     KQALAQLLQAAGYDPGAGGILRYSDHVQGQGPEFFEQACTLGLEGIISKRAAAPYRSG
                     RSKLWLKVKCTTQHAEFVVGGYTPPAGARSGFGALLLGSFRDGQLEYAGRLGTGFSNR
                     QLEQLHATLQQQETELSPFAPSPMLTAARGVHWVKPTLVVAVEYVERTRDGLLRQPSF
                     IGIREDVEPGQVESTGDNLEEAALAAAPRSARHATTGAVEIAGVQLTHPERILYPELG
                     VTKLALARYYEAIHEWVLPYLAQRPLVLLRCPKGRQACFYQKHLANSQAGSVPRIAIP
                     EGGTVRNYVYVRSLADILELVQHGVLEFHAWGCRVEDIEHPDLLVFDLDPAPEVAWQE
                     VLRTARELRERLHGLGLTAFPRTTGGKGLHLVVPLQPDADWESVKSFARAVSEQHARD
                     EPRMLTTNMSMAKRHGRIYLDYLRNTRGSTAIACYSVRARPGAPVAVPLRWDELGPAV
                     RSDHYTVETLQRRLGALRADPWEGFFEMAVPLGAEMLKAVGINEPRPSFRRKPTTVRN
                     KFC"
     gene            complement(2851..3141)
                     /locus_tag="VFW59_06075"
     CDS             complement(2851..3141)
                     /locus_tag="VFW59_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5363814.1"
                     /translation="MRINILSTAERDLEEGYHFYEVQADGLGTYFLDTLYSDIDSLAY
                     FAGVHRIVLGYHRLLSKRFPFAIYYRVANDEVTVFAILDCRRNPSWIREKLS"
     gene            complement(3138..3359)
                     /locus_tag="VFW59_06080"
     CDS             complement(3138..3359)
                     /locus_tag="VFW59_06080"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX5363815.1"
                     /translation="MNRIDVPIAQLSFTQKLDLMEMLWADMASNEKELESPAWHGEIL
                     NERETALNTGKATVSSWQEAKERIRKNVA"
     gene            complement(3446..3736)
                     /locus_tag="VFW59_06085"
     CDS             complement(3446..3736)
                     /locus_tag="VFW59_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX5363816.1"
                     /translation="MGKLHNIHPGEILLEEFLRPMEISQTRLANSIGVPPRRINEIVL
                     GKRGITADTALRLAKVFGTSVQFWMGLQDEYELREARSAIKDQLEHMMPIAA"
     gene            complement(3747..4028)
                     /locus_tag="VFW59_06090"
     CDS             complement(3747..4028)
                     /locus_tag="VFW59_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5363817.1"
                     /translation="MIIGFASRETERIWRAEVSRKLPHEIQQVALRKLFMLDKAQTLN
                     DLRIPPANRLEALKDDRKGQHSIHINDQWRICFVWTSNGPDAVEIVDYH"
     gene            complement(4309..5706)
                     /gene="nrfD"
                     /locus_tag="VFW59_06095"
     CDS             complement(4309..5706)
                     /gene="nrfD"
                     /locus_tag="VFW59_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5363818.1"
                     /translation="MSALAEDKDGIGANPAWLRVRDDALRSLGTPGAKFWGVLALMVC
                     IASIGVACEAYQYNVGMGVGNFNNPHVWSLYVSSFIFWIGMSHSGTLLSAILHITKSE
                     WRKPVYRFAEAMTVFSLATASLFLFIHLGRSWQIYYAIPYPNERGLWPSFQSPLIWDA
                     LAIATYFASSVVFLYVGCIPDFAICRDNTRGWRRRFYGALALEWQGTDRHWRNFRVTY
                     LTIACFMIPLAVSVHSIVATDFAVSQQPGWHVTSYPPYFVAGALYSGCAAIITLFVIL
                     RYAFGFQQYLTLPILRKMVRLTFAMAMVWTYLNCAEFASVWYGPDQAPKDVLLQKMTG
                     VYAPFWWTMIFCGSVLPFVMLVKRLQTSIPAMFTVSLLLNFGMWVERWMIVTPTMAAS
                     YYPWTWDTDQWPSLIQWGIVFGSFGFFCTMLMIFFKFVPSISIYEVAGLAIKEQQADK
                     RYWQRIPAEPGAAPR"
BASE COUNT         1138 a         1806 c         1845 g         1234 t
ORIGIN      
        1 ggttgttgag ttggcgctct gggttctatc ccatacaacc atgcactgca acacttccat
       61 cgggtcaaca aaatttattc cggacagtag tgggcttacg ccggaatgac ggcctgggtt
      121 cattgattcc caccgccttg agcatctccg cccccagcgg taccgccatc tcaaaaaatc
      181 cttcccacgg atcagcccgc agcgccccga ggcggcgctg tagcgtctcc accgtgtaat
      241 ggtcggaacg cactgcgggg ccgagttcgt cccagcgcag cggcaccgcc accggcgccc
      301 ccggtcgggc gcgaaccgag tagcaggcga tggcggtcga gccgcgcgtg ttgcgcaggt
      361 agtcgagata gatcctgccg tggcgtttcg ccatgctcat gttggtggtc agcatgcgcg
      421 gctcgtcccg cgcatgctgt tccgataccg cacgggcaaa gctcttcacc gactcccaat
      481 ctgcatctgg ctgcagcggc acaaccagat gcagtccctt gccgccggtg gtgcgcggaa
      541 atgctgtcag ccccaaccca tgcaggcgct cgcgcagctc gcgtgcggtg cgcagcacct
      601 cttgccaggc cacctcaggt gcgggatcga ggtcgaacac cagcaggtct gggtgttcga
      661 tgtcttccac acggcagccc cacgcgtgga actccagcac gccgtgctgc accagttcca
      721 gaatgtcggc gagcgagcgg acgtagacat agttgcgcac tgttccgcct tccggaatcg
      781 cgatgcgcgg caccgagccg gcctggctat tcgccagatg cttctggtaa aagcaggctt
      841 ggcggccttt cgggcagcgc agcagcacca gggggcgttg cgcgagatag ggcagcaccc
      901 attcgtggat ggcttcgtaa tagcgggcca gcgccagttt ggtcacgccc agttccgggt
      961 agaggatgcg ctcggggtgc gtgagctgga ctccggcgat ctccaccgcg cctgtggttg
     1021 cgtgccgcgc cgagcggggg gcggcggcta acgcggcctc ttccagattg tcgccggttg
     1081 attcgacctg cccgggctcc acgtcttcgc gtatgccgat aaaactgggc tggcgcaaca
     1141 ggccgtcgcg ggtgcgctcg acgtactcga ccgcgaccac cagcgtcggt tttacccaat
     1201 gcacgccgcg cgcagcggtc agcatgggcg agggcgcaaa aggcgatagc tcggtctcct
     1261 gttgctgcaa cgtcgcgtgc aactgctcga gttggcggtt gctgaagccg gtgcccagcc
     1321 gccccgcata ctccagttgc ccatcgcgaa agctgccgag caatagcgcg ccgaagccgc
     1381 tgcgcgcacc ggcgggcggc gtgtagccgc cgacgacgaa ctcggcgtgc tgggtggtac
     1441 atttcacctt gagccacagc ttgctgcggc cgctgcgata gggcgcagcg gcgcgcttgg
     1501 aaatgatgcc ctcaaggcca agcgtgcagg cctgctcgaa gaactccggc ccctgtccct
     1561 gcacatgatc cgaatagcgc agtattccgc cagcgcctgg gtcgtaaccc gccgcttgca
     1621 gcaactgcgc cagcgcctgc ttgcgcacat gcagcggcag cgcagagagg tcgtagccgt
     1681 ccagatagac cagatcgaaa agctgataga cgagctgtgc ggtctgcttg cggctcagcg
     1741 cctcctgcaa ctcgcgaaag ctggtcgcac cgcctgcgct aagcgccacc agttcgccgt
     1801 cgagaatggc ctgctgcacc ggcaacgcct ccagttgccg cgcctgggcg tgcaaccgtt
     1861 ttgtccagtc gtggccgttg cgcgtcatca gccgcgcctt gccgtgctcg atgtgcgcca
     1921 cgatgcgata accgtcgaac ttgatttcgt gcagccaggc ttggccggtc ggcgcgcgtt
     1981 cgccgggagt ggcaagctgc gggggcaggg tggcgggcgg cggcgctttg cgggcatgcg
     2041 gaaagtcttt tgcactgggc ggcggagggc gcgcgccagc gtcgtgatgc tcactgttcg
     2101 cggcgatctc cgccatgctg cggccgcttg ccacgctcag gtcgtcgggg tgcaggtcgt
     2161 gctgcgggtg gtcggtgcgc ttgatcaaca gccactgctt gttctttccg tgcacgtccc
     2221 agtcgcgcat gcgcaccagc gtccaggccc ccttgagctt ggcgccggtg aggatgaagt
     2281 cgatctgctt gtcgtcaaaa cggccatcgg tttgccacag gcccacatcc cacaacatca
     2341 ccgtgccgcc gccgtattcg cccttgggga tgttgccctc gaactcgcca tactcgagcg
     2401 ggtggttctc cacctcgata gcgaggcgct tctcgccgat ctccagcggc gcacccttgg
     2461 gcagcgccca actgcgcagc accccgtcct gctccatgcg cagatcgaaa tgcaaacgcc
     2521 gtgcggcatg tttatgcatc acgtagagat gcccggtttg caccgccgga gccgtgcttt
     2581 cgggttcggg gctgcggcgg aaattgcgct tgtgctggta ttgatcgagg gagggagtcg
     2641 ccatgagttt tcacctgcct ggctgtagtc cctgaatggt agtggtcgtg aagggaacgc
     2701 gtgatgggct gaacgcggta attgcgcggg aaggattgct actgtctgca ctgatgcggt
     2761 tcgagaagta gaaattgcta ccgcacaaaa actactccgg gccgtcggcg acgaggttac
     2821 agggttcgct ggcggagagc gggttttggc tcatgagagt ttttccctta tccagcttgg
     2881 attacgtcgg caatccagta tggcgaatac ggtgacttcg tcgttggcga ctcggtaata
     2941 aatagcgaat ggaaagcgtt tggagagtag ccggtggtag cccagtacga tgcggtgtac
     3001 gcccgcaaag taggcgagtg aatcaatgtc ggagtagagc gtatccagaa aataggtgcc
     3061 gaggccgtcg gcttgtactt cgtagaagtg gtaaccttct tcgaggtcgc gctctgcggt
     3121 gctgaggatg tttatcctca tgcgacgttt ttcctgattc tttctttggc ttcttgccag
     3181 cttgaaacgg ttgctttgcc tgtattcagg gcggtttcgc gctcattgag tatctcgcca
     3241 tgccacgccg gagattcgag ctctttttcg ttgctggcca tgtcagccca aagcatctcc
     3301 atcaaatcca gtttttgggt gaatgaaagc tgggcaatgg gaacatcgat tctgttcatt
     3361 tactactctc ctgtgaatag ttgggagtag gccacgcttt cacgcagcca tgcatgttta
     3421 ttaattccgt cgtgcggcaa gatggctatg ccgcaatcgg catcatgtgt tccagttgat
     3481 ctttgatcgc gctacgggct tcgcgcaatt cgtattcgtc ctgcaaaccc atccagaatt
     3541 gcacggaagt gccgaacacc ttggcgaggc gcagtgcggt gtctgcggtg atgccgcgtt
     3601 tgcccaacac gatttcgttg atgcggcgcg gcggtacgcc tatcgagttc gccagcctgg
     3661 tctggctgat ttccatcggg cgcaagaact cttcgaggag tatttcgccg gggtgtatat
     3721 tgtgtaattt gcccatgttc atctccttaa tggtagtcga cgatctcgac cgcatcgggg
     3781 ccgttgcttg tccacacaaa acagatacgc cactgatcgt tgatgtggat gctgtgctgg
     3841 cctttccggt catctttcaa tgcttcgagc cggttggccg ggggtatcct caaatcgttg
     3901 agcgtttgcg ctttgtcgag catgaacagc ttgcgcaagg cgacttgctg aatctcgtgc
     3961 ggcaatttgc gcgacacttc ggcgcgccaa attctttcgg tttctctgga tgcaaatccg
     4021 ataatcatgc gccagcataa cgaatatcgt tatgggcggc aactgacgtg aatttaatgg
     4081 gacagatcgc gcttatgcgt caacgagcgc gtaaaccttt gcctcccagg ggaagatggg
     4141 ttcgcgcgcg acccaatccg ccggaacgtc gcgatcttgc gtaatttccc gccacttctt
     4201 cccgttgggt gtcgccggcc agttcgacac gcgatactcc gcgttctcgc tgagggcatt
     4261 ggcggtgagc catattcaat tacctccgtt gtgcatggcg cagcccgctt accggggagc
     4321 ggcgccgggt tccgccggta tccgctgcca gtaccgcttg tcagcttgct gttccttgat
     4381 cgcgaggccg gcgacctcgt agatcgaaat cgatggaacg aacttgaaga agatcatcag
     4441 catcgtgcag aagaacccga agctgccgaa cacgatgccc cactggatca ggctgggcca
     4501 ctggtcggtg tcccaggtcc acgggtaata gctggcggcc atggtcggcg tcacgatcat
     4561 ccagcgctcc acccacatgc cgaaattaag cagcaacgac acggtgaaca tcgcggggat
     4621 gctggtctgc agccgcttga ccagcatcac gaacggcagc accgagccgc agaaaatcat
     4681 cgtccaccag aacggcgcgt agacgccggt catcttctgc agcagcacat ccttgggtgc
     4741 ctggtctggg ccgtaccaca ccgacgcgaa ttcggcgcag ttcaggtagg tccacaccat
     4801 cgccatcgcg aaggtcaggc gcaccatctt gcgcaggatg ggcagcgtca ggtattgctg
     4861 gaagccgaac gcgtagcgca ggatcacgaa caacgtgatg atcgccgcgc agccggaata
     4921 cagcgcgccc gcgacgaagt acggcgggta cgacgtcaca tgccagcccg gctgctgcga
     4981 cacggcaaaa tcggtcgcga cgatggaatg caccgacacc gccagcggga tcatgaagca
     5041 ggcgatcgtc aaataggtca cgcggaaatt ccgccagtgg cgatccgtcc cctgccattc
     5101 cagcgccagc gcaccgtaga agcgcctgcg ccagccgcgc gtgttgtcgc ggcagatcgc
     5161 aaagtcgggg atgcagccga cgtagaggaa cacgaccgac gacgcgaagt aggtcgcgat
     5221 cgccagcgca tcccagatca gcggcgactg gaagctgggc catagcccgc gttcgttggg
     5281 gtaggggatc gcatagtaaa tctgccatga ccggccgagg tggatgaaca ggaacaggga
     5341 agcggtcgcc agcgagaaca ccgtcatcgc ctcggcaaag cggtagaccg gcttgcgcca
     5401 ttcggacttg gtgatgtgca ggatcgccga cagcagcgtg ccggaatggc tcatgccgat
     5461 ccagaagatg aacgacgaca catagagcga ccacacgtgc ggattgttga aattgcccac
     5521 gcccatgccg acgttgtact gataggcctc gcacgccacc cctatggagg cgatgcagac
     5581 catcagcgcg agcacgcccc agaattttgc gcccggcgtg cccaggctcc gcagcgcatc
     5641 gtccctgacc cgcagccacg cggggtttgc ccctatgccg tccttgtcct ctgcaagcgc
     5701 gctcattgtc acctctggtt ttttgaggtg acaagtagag gcgcgtatcg gtaacgaatc
     5761 aatcaggtga attgcgtagg catcgcagac tccggcgttt tcttgtcgcc tatcgcgcac
     5821 cggtttgcgg cgagcttgcc ttggaaccag actggaaacc tatgcgtccg ccttcggctc
     5881 caacggtaat ccccccagaa attagcggca gttaaaagta gaattttctc aaatagaaag
     5941 cgaggaagtt ttacatgaaa aaatcacgtt tttcagacag ccagatcatt gccgtactca
     6001 agcaagccga ggctggcagc ccg
//
LOCUS       DASUIV010000163         5509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1154676, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000163 DASUIV010000000
VERSION     DASUIV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154676"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFW59_06100"
     CDS             complement(<1..564)
                     /locus_tag="VFW59_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX5363819.1"
                     /translation="MKMKKLAGLISLLCMSPAWAIDPFTVKDIRVEGIQRTEAGTVFS
                     YLPVKVGDTMDDSQAAAAIKALYGTGFFKDVRLEVEQGVLIVLVRERPSIASIEVNGV
                     KDFPKDQLKDNMKYAGLAEARIFDKGALDKATQELKRQYVARGKYGVSVTTSVTELER
                     NRVGIVFNVVEGEVSKIKQINIVGNSAY"
     gene            complement(571..1926)
                     /gene="rseP"
                     /locus_tag="VFW59_06105"
     CDS             complement(571..1926)
                     /gene="rseP"
                     /locus_tag="VFW59_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX5363820.1"
                     /translation="MTTLLAFVGAIALLVVFHELGHYWVARRCGVKVLRFSIGFGKPI
                     YIKRFAGSDTEWTVSAIPLGGYVKMLDEREGEVAEDELQYTFNRKPVLQRMAIVVAGP
                     LANFLLAIVLYWALFIHGVPGLKPTLGDVPNGTPAAAAQLQPGETIVSINGEPVPSWQ
                     EVRWMLLELALQKAEARIEAQTASGSTALHALNLSGLEPKDLDGEFLDKLGMHLYQPV
                     IQPVIGKLTEGSVAQRAGLQEGDVILRVNGAAVAKWNEVVDAVRAHPGQTVRLEIRRG
                     GTVAEIAVIPQAVTESGKTVGKIGAGPQVDKAAWQAMFIEVSYSPLDAFGQSLRKTWE
                     TSAISLKMMGKMVMGEVSMKNLSGPITIADYAGQSAAMGAVAYLGFLALISISLGVLN
                     LLPIPLLDGGHLLYYVAELLKGSPVSEQAWEIGQKIGIALLGTLMVFAIYNDINRLIS
                     G"
     gene            complement(1923..3101)
                     /gene="ispC"
                     /locus_tag="VFW59_06110"
     CDS             complement(1923..3101)
                     /gene="ispC"
                     /locus_tag="VFW59_06110"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX5363821.1"
                     /translation="MQHLTILGSTGSIGTSTLDVVARHPDKFKVVALTANSQTDLLFR
                     QCVQFKPRFAVLLDEAAAAQLRQRMREAGLGTEVLCGVRALEEVSALPEVDAVMAAIV
                     GAAGMRPTLAAARAGKKILLANKETLVMAGHVFMDAVRASGSALLPIDSEHNAIFQAL
                     PRGYDGDMARNGVRRILLTASGGPFRNTPLSELHSVTPDQACAHPNWSMGRKISVDSA
                     SMMNKGLEVIEAHWLFNASADDIQVVVHPQSVIHSLVEYVDGSVLAQLGNPDMRTPIA
                     YGLAYPERIDSGVASLDLFKVATMNFVAPDFERFPCLGLAYQALRAAGTAPAMLNAAN
                     EVAVAAFLDSRISFLSIPRVIETVLSALPVNPVHGLDDVLAADAEARSVAQQQVLKLA
                     "
     gene            complement(3104..3928)
                     /locus_tag="VFW59_06115"
     CDS             complement(3104..3928)
                     /locus_tag="VFW59_06115"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5363822.1"
                     /translation="MLKSRVITAVVLLVLLLSALFFLPAMAWDALVVVMVMQGASEWA
                     KLAKLPGRNANVFWGLLLLTMAALLWFDASHTENEQVYMHLLVYAVSALLWLFVVPTW
                     LMIGWKVKQPGLMALVGWSVLIPTGLAMLDLRAVSPWLLLFVMGLVWVADSAAYFAGR
                     KFGKHKLAPSISPGKTWEGVAGAIAGVTVYVLLVWSFGPVAVHREVLPILLLAAWWWV
                     GLAVIGDLFESAIKRQAGVKDSGALLPGHGGLLDRIDALTSTLPLAAMVILFQRLG"
     gene            complement(3921..4727)
                     /locus_tag="VFW59_06120"
     CDS             complement(3921..4727)
                     /locus_tag="VFW59_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX5363823.1"
                     /translation="MALFKSSTRIIPDAPAVPRHIAVIMDGNGRWAKQRFMPRVAGHQ
                     RGVEALREVVKACRDMGVEYLTVFAFSSENWRRPADEVSFLMSLFLKMLEREVARLHK
                     NNIRLKIIGDRSRFDDKLMQAMLDAETLTANNTGLTLTIAANYGGRWDMMHAVQTMLR
                     EHPQLAQTFTEEDLQPYLSMSDAPEPDLFIRTGGEQRISNFMLWQLAYTELYFTDTLW
                     PAFDRSELDKAVASYQKRERRFGRTSEQLRGEQLPAEQTAVEPPQGSVDA"
     gene            complement(4795..5352)
                     /gene="frr"
                     /locus_tag="VFW59_06125"
     CDS             complement(4795..5352)
                     /gene="frr"
                     /locus_tag="VFW59_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5363824.1"
                     /translation="MIADIKKTTEQKMNKSLDALKADLGKVRTGRAHTGLLDHVMVDY
                     YGSPTLVTQVANVTLIDARTIGVQPWEKNMVGPVERAIRDSDLGLNPATNGDMIRVPM
                     PMLTEERRRDLIKVVKAEGENAKVAVRNIRRDANNHLKEALKKKEIAEDEERRTQDEI
                     QKLTDRFVAEIDKALAAKEVDLMAV"
     gene            complement(5368..>5509)
                     /locus_tag="VFW59_06130"
     CDS             complement(5368..>5509)
                     /locus_tag="VFW59_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5363825.1"
                     /translation="LQVMDATALTLCRDQKLPIIVFSIFKPGALKRVVFGEGEGTLVR
                     CD"
BASE COUNT         1065 a         1848 c         1551 g         1045 t
ORIGIN      
        1 ataggcgctg ttgccgacga tattgatctg cttgatcttg gatacttcgc cttccaccac
       61 gttgaacacg atgccgacgc ggttgcgctc cagctcggtc acgctggtcg tcacgctgac
      121 gccgtacttg ccgcgcgcca cgtactggcg cttcagctcc tgcgtggcct tgtccagcgc
      181 gcctttgtcg aaaatgcgcg cctcggccag cccggcgtat ttcatgttgt ccttgagctg
      241 gtccttgggg aaatccttca cgccgttgac ttcgatgctg gcaatcgacg ggcgctcgcg
      301 caccagcacg atcagcacgc cctgctcgac ttccaggcgc acgtccttga aaaatccggt
      361 gccgtacaaa gccttgatcg ctgcggcggc ctggctgtca tccatggtat cgccgacctt
      421 gaccggcaga tagctgaaca ccgtaccggc ctcggtacgc tgaattcctt cgacgcgaat
      481 atccttgacg gtgaatggat cgatggccca agcgggagac atacaaagca gggaaatcaa
      541 ccctgccagt ttcttcatct tcatctgcaa ttaacctgaa ataaggcgat ttatatcgtt
      601 atatatggca aagaccatca gcgtgccgag cagcgcaatc cctatcttct gcccgatctc
      661 ccatgcctgc tcggaaaccg gactgccctt gagcaactcg gcgacataat acagcaagtg
      721 tccgccatcc aataagggga tgggcagcaa attcagcacg ccgaggctga tgctgatcag
      781 cgccagaaaa cccagatagg ccaccgcgcc catcgcggcg gactgcccgg cgtaatcggc
      841 aatcgtgatc ggcccgctca ggtttttcat cgacacctcg cccatcacca tcttgcccat
      901 catcttcagg ctgatggcgg aggtctccca ggtcttgcgc agcgactggc cgaaagcgtc
      961 gagcggcgaa tagctcacct cgatgaacat cgcttgccat gcggccttat cgacctgcgg
     1021 accggcgccg atcttgccga ccgttttgcc ggattcggtg acggcttgcg ggatcaccgc
     1081 tatttccgca accgtcccgc cgcgccggat ttccagccgc acagtttgcc ccggatgcgc
     1141 gcgcaccgca tcgacgacct cgttccactt cgcgactgcc gcgccattca cccgcaggat
     1201 gacatcgccc tcttgcagcc cggcgcgttg cgccacgctg ccttcggtga gcttgccgat
     1261 gacgggctga atgacaggct ggtagagatg catgccgagc ttgtcgagaa actcgccgtc
     1321 caggtctttc ggctccagcc cgctcaggtt cagcgcgtgc agcgccgtgc tgccgctggc
     1381 ggtttgcgct tcgattctcg cttcggcctt ttgcagcgcg agttccagca gcatccagcg
     1441 cacttcctgc cagctcggca cgggctcgcc attgatgctg acgatggtct cgcccggctg
     1501 caattgcgcg gcggcagccg gtgtgccgtt cggcacgtcg cccagcgtcg gcttcaagcc
     1561 cggcacgccg tgtatgaaca aggcccaata cagcacgata gccagcaaaa aattcgccag
     1621 cggcccggcc accacaatcg ccatgcgctg caacaccggc ttgcggttga aggtgtattg
     1681 caactcatct tctgcgacct cgccttcgcg ctcgtccagc atcttgacgt agccgcccag
     1741 cgggatcgcg gaaactgtcc attcggtatc gctgccggcg aagcgcttaa tgtaaatcgg
     1801 cttgccgaac cctatcgaga agcgcaacac cttcacgccg caacggcgcg cgacccagta
     1861 gtgccccagc tcgtggaaca ccaccagcag cgcgattgcg ccgacgaagg ctaacaaggt
     1921 ggtcatgcga gtttcagaac ctgctgctgc gcaacgctgc gcgcctcggc atccgccgcc
     1981 agcacatcgt ccagaccatg caccggattg accggcaacg ccgacagcac cgtctcgata
     2041 acgcgcggaa tcgacaggaa ggaaatccgg ctatcgagga atgccgcgac cgcgacttcg
     2101 ttcgccgcgt tcagcatcgc cggagccgtg ccagccgcgc gcaacgcctg atacgccaag
     2161 cccaggcagg ggaagcgctc gaagtccggc gcgacgaaat tcatcgtcgc caccttgaac
     2221 agatccagcg aggcgacgcc ggagtcaatg cgctccgggt aagccaggcc ataggcgatc
     2281 ggcgtgcgca tatcggggtt gccgagctgc gccagcaccg agccgtccac atattcgacc
     2341 agcgaatgga tcacgctctg cggatgcacc accacctgaa tgtcgtcggc gctcgcgttg
     2401 aacagccagt gcgcctcgat gacttccagc cccttgttca tcatgctcgc cgaatccacc
     2461 gaaatcttgc gccccatgct ccagttcgga tgcgcgcagg cctgatccgg cgtgacgctg
     2521 tgcagctcgc tcagcggcgt gttgcggaac gggccgcccg atgcggtgag caggatgcgg
     2581 cgcacgccgt tgcgcgccat atcgccgtcg tacccgcgcg gcaacgcctg gaagatcgcg
     2641 ttgtgttcgc tgtcgatggg cagcagcgcc gaaccgctgg cgcgcaccgc atccatgaac
     2701 acatgccccg ccatcaccag cgtttccttg ttcgccagca aaattttctt gcccgcacgc
     2761 gcagcagcca gcgtcgggcg catgcctgcc gcgccgacaa tggccgccat caccgcgtcc
     2821 acttcgggca gcgcggaaac ttcctcgagc gcccgcacgc cgcacaacac ctcggtgccc
     2881 aaccctgcct cacgcatgcg ctggcgcaac tgcgcggcgg cagcctcatc cagcagcact
     2941 gcaaaacgcg gcttgaactg cacgcattgc cggaacagca aatcggtctg gctgttggcc
     3001 gtcagtgcga cgaccttgaa tttatcggga tggcgggcca ccacgtccag cgtgctggtg
     3061 ccgatactgc ctgttgaacc caggatggtt aagtgctgca cggctacccc aatctctgaa
     3121 acaaaatcac catcgccgcg agcggcaacg tcgaagtcag cgcgtcgatg cggtccagca
     3181 aaccgccgtg gccgggcaac aatgcgccgc tatccttcac gcctgcctga cgcttgatcg
     3241 ccgactcgaa caggtcgccg atcaccgcca gcccgaccca ccaccacgcg gccagcaaca
     3301 ggataggcag cacctcgcga tgcacggcaa cgggcccgaa gctccacacc agcagcacat
     3361 agaccgtgac gcccgcgatt gcgcccgcga caccttccca ggtcttgccg gggctgatgc
     3421 tgggagccag cttgtgcttg ccgaacttgc gtccggcgaa atatgccgcg ctgtccgcga
     3481 cccacaccag ccccatcacg aacagcagca gccaagggct gaccgcgcgc agatcgagca
     3541 tcgccagccc ggtcgggatc agcaccgacc agccgaccag cgccatcagg ccgggctgct
     3601 tgaccttcca gcctatcatc agccaggtcg gcacgacgaa cagccacagc aacgccgata
     3661 cggcatacac cagcagatgc atgtatacct gttcattctc cgtatgggag gcgtcgaacc
     3721 agagcagcgc ggccatcgtc agcagcagca ggccccagaa aacattcgca ttgcggcccg
     3781 ggagctttgc cagcttcgcc cactcggacg cgccctgcat caccatcacg accaccaacg
     3841 catcccacgc catggcgggc aggaaaaaca gcgccgacaa caacagcacc agcaaaacaa
     3901 ccgccgtaat cacgcgcgac ttaagcatca acactccctt gcggcggttc aaccgccgtc
     3961 tgttcggcgg gcaactgctc gccgcgcaac tgttcgctgg tccggccaaa acgccgctcg
     4021 cgtttttgat aagaagcaac cgccttgtcc agctcgctgc ggtcgaaagc gggccacagc
     4081 gtgtcggtaa agtacagctc ggtatacgcc agttgccaca gcatgaaatt gctgatgcgc
     4141 tgctcgccgc cggtgcggat gaacagatcg ggctcgggcg catcgctcat cgacaggtag
     4201 ggctgcaaat cttcttcggt aaaggtctgc gccagttgcg ggtgctcgcg cagcatcgtc
     4261 tgcacggcat gcatcatgtc ccagcgaccg ccgtaattcg ccgcgatggt cagcgtcagg
     4321 ccggtgttat tggccgtcag cgtctccgca tccagcatcg cttgcatgag cttgtcgtcg
     4381 aagcggctgc ggtcgccgat aattttgagg cggatattgt ttttgtgcag tctggcaact
     4441 tcgcgttcca gcattttcag gaacagcgac atcaggaagg aaacttcgtc ggcgggacgc
     4501 cgccagtttt cgctgctgaa cgcgaacacg gtcaggtact ccactcccat gtcgcggcag
     4561 gctttcacca cttcgcgcaa cgcctccaca ccgcgctgat gaccggccac gcgcggcatg
     4621 aagcgctgct tcgcccaacg cccgttgccg tccatgatga cggcgatatg gcgcggcact
     4681 gcgggagcgt cggggatgat gcgggtggag cttttgaata aagccatttc aggatcggag
     4741 atcgaggagc gagaaccggg aacgctcaac gagcatcccc ggctctccat cccgtcagac
     4801 cgccatcaaa tcgacttctt tggcggctag cgccttgtcg atttcggcga caaagcggtc
     4861 ggtgagtttc tggatttcat cctgggtgcg gcgctcttcg tcttcggcga tttccttctt
     4921 cttgagcgct tctttcagat gattgttggc gtcgcggcgg atattgcgca cggcgacctt
     4981 ggcgttctcg ccttcggcct tgaccacctt gatcagatcg cggcggcgct cttcggtcag
     5041 catcggcatc ggcacgcgaa tcatgtcgcc gttggtagcc ggattcagcc ccagatcgga
     5101 atcgcgaatc gcgcgttcga ccgggccgac catattcttt tcccacggct gaacgccgat
     5161 ggtgcgcgcg tcgatcagcg tgacgttggc gacttgcgta accagtgtcg ggctaccgta
     5221 ataatccacc atcacatggt ccagcagacc ggtatgcgcg cgcccggtgc gcaccttgcc
     5281 gaggtcggct ttcagcgcat ccagcgactt gttcatcttt tgttcggtcg tttttttgat
     5341 gtccgcaatc atggtttatc tccgaaatca atcacagcgc actaatgtgc cttcgccttc
     5401 gccaaacacc acgcgcttca gagcgccggg cttgaaaata ctaaacacga tgataggcag
     5461 cttctggtcg cggcacagcg tcaacgccgt cgcgtccatc acctgcaga
//
LOCUS       DASUIV010000164         5437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_874392, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000164 DASUIV010000000
VERSION     DASUIV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874392"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFW59_06135"
     CDS             complement(<1..494)
                     /locus_tag="VFW59_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5363826.1"
                     /translation="MTADTSLLATLAAIVGDGGMLTGDAATPYCTDWRGRYRGNALAV
                     VFPADTAQVSEVVGLCARHDIAIVPQGGNTSLCGASVPLAGSSPQMVVNLSRMNRIRA
                     LDATNYTMTVEAGCKLASLYEAAEQAERLFPLGLTAIAPHCEIGGNLSTNAGGIGVLR
                     YGNAR"
     gene            complement(499..1251)
                     /locus_tag="VFW59_06140"
     CDS             complement(499..1251)
                     /locus_tag="VFW59_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor-1 alpha"
                     /protein_id="HEX5363827.1"
                     /translation="MEAIQRFTLADLSLPVKTLFTGYLLAIGLGLLVAGAQIMLTHGM
                     ADGKFGLSVDDIVYSYHGNHDGANASKLEAKLYGSMKDNAPPEVRLALLKWAHAGAPE
                     GEWEGSIKPQVEQYCAPCHANMPGLANIADKAVMDTMTKRDEGQSFATLTRVSHIHLF
                     GVSFIFFFVGWIFTYATGFSPMAKAILIFTPFGFLMVDVISWWLTKLDPNFAWLVIIG
                     GFGYSIASTIMICTSLYQMWIMPLRKLKAPAA"
     gene            complement(1317..3512)
                     /locus_tag="VFW59_06145"
     CDS             complement(1317..3512)
                     /locus_tag="VFW59_06145"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5363828.1"
                     /translation="MPIVRVALDVPLPALFDYSTEERAAPGQRVIVPFGRRQMVGVVM
                     ECVATSDMDPERIKPVQQLLHDGAPLSAELLDLLRFCSDYYRHPVGQVVVSALPTRLR
                     SDKPLAVKPPENYRLSAAGAALDLDSFPGRRVVQRRILVRLSEGAASLSQLRDLSATA
                     GAQLKALLAEGYVEHCEDAVVVSGHKLVGAHRLTDEQQQAVDAVSEAQGYGCFLLHGI
                     TGSGKTEVYVHLMHRVLQPCPERGLRDGGQVLLLVPEINLTPQLEQYFRSRFPDTSFA
                     SLHSGLSEGERLHNWQQAQSGAAQIVLGTRLAVFAELPRLALIIVDEEHDGSFKQQDG
                     LRYSARDVAIFRASQHKVPIVLGSATPSLESYHNAQSGRYRMLKLSGRALATASLPAV
                     RCVNVNQTVMHHGISENLLREIELRIARREQSLLFINRRGYAPVLMCAGCGWLSSCPN
                     CAGKLVLHLKDRRLRCHHCGHQTRVPQACPSCGNADLQPVGSGTQRVEDVLQERFPGA
                     RILRVDRDSTRNKRAWQTMREQIHANEVDILVGTQMLAKGHDFPALTLVGVLNPDSAL
                     YSSDFRAPERLFAQLAQVAGRAGRADKPGEVLIQTAFPDHPLYRALQQHDFDGFAATQ
                     LAERQMAGFPPFVFQAMLRAEGRQEGEVFAFLNQARAAAVDLQAEVEVFGVVPAALAR
                     RANHVRAQLLIQGATRKALQQFMRAWQPALDALPSQKLRWSLDVDPQEF"
     gene            3766..4587
                     /locus_tag="VFW59_06150"
     CDS             3766..4587
                     /locus_tag="VFW59_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5363829.1"
                     /translation="MPAFPKSVRRILELSLDINCSPKELVAVIEKDPVMTMKLLRALN
                     SAYYSFPKQITSVNQAVVYLGLNTIKNMALSFAALGTLPKNNAAGFDMQRYLLHSLTT
                     AGLARLLAQKFNVADANPADCYIAGLLHDFGKVVFAQSMPEEFKQALEKSAAESMPLH
                     LAEQAVIGSDHTEIGAMLVEKWQFPIGISTLIREHHSLSARHTGVAACLFVANQISKQ
                     LGYATSDYLNIDEMPAELVAQFGGDLPEVIASLGDISTLLQEAQAYAQAYQEPNT"
     gene            4584..>5437
                     /locus_tag="VFW59_06155"
     CDS             4584..>5437
                     /locus_tag="VFW59_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5363830.1"
                     /translation="MKVKFWGVRGSIPAPGPNTVRYGGNTTCIEVRSDEDDLIILDGG
                     TGIFPLAQSLLKSLPITAHIFNTHSHWDHIQGLPFFIPIFIPGNKIYLYGAVDPVSGE
                     GPERIMNVQMQYSFFPVREAEMKSRIEYVTAMPHQPVMVGKTKITPTMLNHPVVNLGY
                     RVESNGKSVFFTGDHEPYANIYAPGDEGNAEYARLITDQRASVIDAIRGVDVLIADSS
                     YTIEEHPQKLGWGHGTFDSSIELAIAAGAKILYCTHHEPTRSDDDLERAFAAALARYA
                     TALNGLDVR"
BASE COUNT         1164 a         1734 c         1552 g          987 t
ORIGIN      
        1 cgcgcgttgc cgtagcgcag cacgccgatg ccgcccgcgt tggtggaaag attgccgccg
       61 atctcgcagt ggggcgcgat ggcggtgagg ccgagcggaa acagccgctc ggcctgttct
      121 gccgcctcgt acagcgaggc cagcttgcag cccgcctcga cggtcatggt gtagttggtc
      181 gcatccagcg cacgaatgcg attcatccgc gacaggttga cgaccatttg cggcgaactg
      241 ccagccagcg gcacggaagc cccgcacagg ctggtgttgc cgccctgcgg cacgatggcg
      301 atatcgtgcc gcgcgcacag ccccaccacc tcgctgactt gagcggtatc ggcggggaac
      361 accaccgcca gcgcattgcc gcgatagcgc ccgcgccagt ccgtgcaata aggtgttgcc
      421 gcatcccccg tcaacatgcc gccgtcgcca acgatggcgg cgagcgtcgc gagcaggctg
      481 gtatcggctg tcattggact aggccgctgg cgccttgagt ttgcgcaacg gcatgatcca
      541 catctgatac agcgaggtgc agatcatgat ggtcgacgcg atgctgtaac cgaaaccgcc
      601 gatgataacc agccaggcga agttaggatc cagcttggtc agccaccagg agatcacatc
      661 gaccatcaga aatccgaacg gcgtgaagat caggatcgcc ttggccatcg gggagaaccc
      721 agtcgcatag gtgaatatcc agccgacgaa gaagaagatg aaggagacgc cgaacagatg
      781 gatgtgcgac acgcgggtca gcgtggcgaa cgactgcccc tcgtcgcgtt tggtcatcgt
      841 atccatcacc gccttatcgg cgatgttggc caatcccggc atattggcgt gacatggcgc
      901 gcaatactgc tctacctgcg gcttgatgct gccttcccac tcgccctcgg gtgcaccggc
      961 atgcgcccac ttgagcaggg ccagccgcac ctcaggcggc gcattgtctt tcatcgagcc
     1021 gtagagttta gcctccagct tggaagcatt cgcgccatcg tggttgccgt ggtagctgta
     1081 cacgatgtca tccacagaca gaccgaactt gccgtccgcc atgccgtggg tcagcatgat
     1141 ctgcgccccg gccaccagca gccccagacc gatcgccagc aaatagccgg tgaacaatgt
     1201 cttaacgggc aaggaaagat cggccagggt gaaacgctga atcgcttcca ttttatattc
     1261 cgggtaattg aactgcggcc attatatcgc tccgcgcgac cataccggtc ggcgcattag
     1321 aactcctgcg gatcgacatc cagcgaccag cgcaactttt gcgagggcag cgcatccagc
     1381 gccggctgcc aggcacgcat gaattgctgc aaggccttgc gtgtcgcccc ctggatcagc
     1441 agctgcgcgc gcacgtgatt ggcgcggcgc gccagtgcgg cgggcaccac gccaaagacc
     1501 tcgacctcgg cctgcaagtc gacggcggcg gctcttgcct gattgaggaa ggcaaacacc
     1561 tcgccttcct gcctcccttc ggcgcgcaac atcgcctgaa acacgaacgg cgggaatccc
     1621 gccatctgcc gctcggcaag ctgtgtcgcg gcgaagccgt cgaaatcgtg ctgctgcagc
     1681 gcgcgataca gcgggtgatc ggggaaggca gtctggatca acacctcgcc gggtttgtcg
     1741 gcgcgcccgg cccggcccgc cacctgcgcc agctgcgcga acagccgctc cggcgcacga
     1801 aagtcgctgc tgtacaacgc actatccgga ttgagcaccc ccaccagcgt caacgccgga
     1861 aagtcgtgcc ctttggccag catttgcgtg ccgaccagaa tatccacctc gttggcgtga
     1921 atctgttcgc gcatggtttg ccaggcgcgc ttgttgcgcg tgctgtcgcg gtcgacgcgc
     1981 agaatccgcg ccccgggaaa acgctcctgc agcacatcct ccacccgctg cgtgccgcta
     2041 ccgaccggct gcagatcggc gttgccgcaa ctcggacagg cttggggcac gcgcgtctga
     2101 tgaccgcaat gatggcaacg caggcgtctg tcctttagat gcaacaccag cttgcccgcg
     2161 cagttcgggc aggacgacag ccagccgcaa ccggcacaca tcagcacggg cgcatagccg
     2221 cgccggttga tgaacagcag actctgttcg cggcgagcga tgcgcagctc gatctcgcgc
     2281 aacagattct cgctgatacc gtgatgcatc acggtctgat tgacgttgac gcagcgcacc
     2341 gcaggcagac tggccgtagc caatgcgcgg ccactgagct tgagcatgcg gtagcgcccg
     2401 ctctgcgcat tgtgataact ctccagcgaa ggcgtggccg acccgagcac gatgggaacc
     2461 ttgtgctgac tggcgcgaaa gatcgccaca tcgcgcgccg aatagcgcaa cccatcctgt
     2521 tgcttgaacg agccgtcgtg ttcctcgtcg acgatgatca gcgccagcct gggcagctcg
     2581 gcaaacaccg ccagccgcgt gccgagcacg atctgcgccg cgccggactg cgcctgctgc
     2641 cagttatgca gacgctcgcc ctcggagagc ccgctgtgca ggctggcaaa ggaagtgtcc
     2701 gggaaacggc tgcggaagta ttgctccagt tgcggcgtga ggttgatctc cggaaccagc
     2761 aacaacacct gcccgccgtc ccttagaccg cgctcgggac aaggctgcaa aacgcgatgc
     2821 atcagatgca catacacctc ggtcttgccg ctgccggtga tgccatgcaa caggaagcag
     2881 ccatatccct gcgcctcgct gaccgcatct actgcctgct gctgctcatc ggtcagcctg
     2941 tgcgccccga ccaatttgtg gccggataca acgaccgcat cctcgcaatg ttcgacatag
     3001 ccctccgcca gcagcgcctt cagctgcgca cccgccgtgg cagataaatc cctgagctgc
     3061 gaaaggctgg ctgcgccttc ggacagtctg acaagaatgc gccgctgcac gacccggcgc
     3121 ccggggaaac tgtcgagatc gagcgccgca cccgccgcac tcaaacgata attttcgggt
     3181 ggctttaccg ccagcggctt gtccgaacgc aatcgcgtcg gcagtgcgga caccaccacc
     3241 tgccccacag gatgacggta ataatcgctg cagaaacgca acagatcgag caactccgcc
     3301 gacagcggcg cgccatcgtg cagcaactgt tgcaccggct tgatgcgttc cggatccatg
     3361 tccgaagtcg cgacgcattc catcaccacg ccgaccatct gcctgcgccc gaacggcacg
     3421 atcacccgct gaccgggcgc ggctcgctct tccgtcgagt aatcgaacag ggctggcaac
     3481 ggaacatcaa gcgcgacgcg gactataggc ataggcctcg attgtcgcag aaatgcaaaa
     3541 tccgcagaag gaagtcctcg atgcgttttc aaatcgggtt aatattctcg gcatgaaatg
     3601 caagaccgaa tcccatgcgg aacttgggta tttgtaccgc ataaaaaatt taaatcatgt
     3661 ctcattccaa attaagttca gcgattacaa aatatttgta caatgcccgg catgattgat
     3721 acctctaccg actcaacaag caaacttgtt tctgcggtcg aaaaaatgcc ggcctttccg
     3781 aaaagcgtac gacgcatttt ggagttgtca ctcgacatca attgctcccc caaggaattg
     3841 gtggcggtca tcgagaaaga ccccgtgatg accatgaagt tgttgcgcgc actcaactcg
     3901 gcttattaca gctttcccaa gcagatcacc tcggtcaatc aggccgtggt ctatctcggg
     3961 ctgaatacga tcaagaacat ggccctgtct ttcgcggcgc tgggcaccct gcccaagaac
     4021 aatgccgccg gattcgacat gcagcgctat ttgctgcact ccttgacgac cgccggcctt
     4081 gcccgcctgt tagcgcagaa attcaacgtg gcggacgcca atccggcgga ttgttatatc
     4141 gccggactgc tgcacgactt cggcaaggtg gtgtttgcgc aatccatgcc tgaagaattc
     4201 aagcaggcgc ttgagaaaag cgccgcagaa tcgatgccac tgcatctcgc agagcaagcg
     4261 gtgatcggct cggatcacac cgagatcggc gcgatgctgg tggagaagtg gcaattcccg
     4321 atcgggatat ccacgctgat acgcgagcat cactcgctct ccgcccgcca caccggtgtg
     4381 gcagcctgcc tgttcgtcgc caatcagatc agcaaacagc tgggatacgc cacatcggat
     4441 tatctgaaca tcgacgagat gcctgcagaa ttagtcgcgc aattcggcgg agatctgccg
     4501 gaggtgatcg ccagcctggg agatatttcc acgctgctgc aagaggcgca ggcttacgca
     4561 caggcatatc aggaacccaa cacatgaaag taaaattctg gggggtgcgc ggctccatcc
     4621 ccgctcccgg ccctaacacc gtgcgttatg gcggcaacac cacctgtatc gaagtgcgct
     4681 cggacgagga tgacctgatc attctggatg gcggcaccgg aatattccca ttggcgcaat
     4741 cgctgctcaa gtccttgccc atcaccgctc acattttcaa tacgcacagc cactgggacc
     4801 atattcaggg actcccgttt ttcattccga tcttcattcc cggcaacaaa atctatctgt
     4861 atggcgcggt cgatcccgtc agcggggagg gcccggaacg catcatgaac gtacagatgc
     4921 aatacagctt ctttccggtg cgggaagcgg agatgaaatc gcgcatcgaa tatgtgacgg
     4981 cgatgccgca tcaaccggtg atggtgggca agacaaaaat cacccccacc atgctcaatc
     5041 atccggtagt caaccttggc tatcgcgtgg aaagcaacgg caagtcggtg ttcttcactg
     5101 gcgaccacga accctacgcc aacatctatg cacccggcga cgaaggaaat gccgaatacg
     5161 cccggctcat taccgaccag cgggcatccg tcatcgacgc tatcaggggc gtggatgtgc
     5221 tgatcgccga cagctcctac accattgagg aacacccgca aaaactgggc tggggacacg
     5281 gcaccttcga cagcagcatc gagctggcga tcgcggcggg cgcaaaaatc ctgtactgca
     5341 cccaccatga accgacgcgc tcggacgacg acctggagcg agccttcgcc gccgcactag
     5401 ccagatacgc gaccgcacta aacggactgg atgtgcg
//
LOCUS       DASUIV010000165         7749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_967725, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000165 DASUIV010000000
VERSION     DASUIV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967725"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1457
                     /locus_tag="VFW59_06160"
     CDS             78..1457
                     /locus_tag="VFW59_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5363831.1"
                     /translation="MQDNKDSMTPVERRASFGLAGIYGLRMLGLFIILPIFALYAEHL
                     PGGESHMLIGIALGAYGLTQAILQIPAGWLSDRYGRKPVIYAGLALFAIGSFIAASAD
                     DIYWIIAGRVVQGAGALNAAVMALTADLTREEHRTKAMAMIGMTIGITFSLSLILSPL
                     LNSVLGVPGIFALTGALAIAAMLVVKFVIPDPAITRFHTDTEAGHGRFGEVLRNKDLL
                     RLDFGIFSLHAILMSVFMQVPFVLQADGLDVAQHWQIYLPVMLIAFVLMVPPIIIAEK
                     KAKMKQVFMGAVALALVAQALLMFAQHSLWGVAAALLIFFTAFNVLEATLPSLISKIA
                     PLASKGTAMGVYSSVQFLGAFFGAAAGGALMQFVGHDAVFWFSIVLLALWLVVASGMR
                     PPAAVRTRMYHLAEMTEAEGAQLQQRLAQLQGVREALVVAAEGMACLKVEMQGFDEAA
                     VEQLVIKGA"
     gene            1459..1944
                     /locus_tag="VFW59_06165"
     CDS             1459..1944
                     /locus_tag="VFW59_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX5363832.1"
                     /translation="MSVNKVILVGRLGKDPETRYMTNGEAVTNVSLATSENYKDKSGE
                     KQERTEWHNLVFYRRLAEVAGEYLKKGSQVYVEGRIQTRKWQDKEGKERYTTEIIVNE
                     MQMLGGKSTGGSFEVMDQSAPERSAPERSAPARSAAPAARQAPAPAKSSFDNFDDDIP
                     F"
     gene            complement(2030..2884)
                     /gene="rpoH"
                     /locus_tag="VFW59_06170"
     CDS             complement(2030..2884)
                     /gene="rpoH"
                     /locus_tag="VFW59_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX5363833.1"
                     /translation="MNATICFPVPSTVGSLEAYIQSVNRFPVLSQEEEFAYATRFKTE
                     NDLDAARQLVLSHLRVVVSVARGYAGYGLPQADLIQEGNIGLMKAVKRYDPARGVRLV
                     SFALHWIRAEIHEFVVRNWRLVKIATTKAQRKLFFNLRSMKQGLEPLKPQQISEIARQ
                     LNVSEHDVVEMESRFAGHEVALEPTGDSDDESYSPIQYLSDDAEHEPSHILETAEREH
                     LQTTGLANALANLDGRSRRIVEARWLSEGNAATLHELAAEFNVSAERIRQIEQKALSK
                     MQTALSAA"
     gene            3181..4485
                     /locus_tag="VFW59_06175"
     CDS             3181..4485
                     /locus_tag="VFW59_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363834.1"
                     /translation="MVVNSVECMPYKLTAIATLVAMLYAPCARSEEPDAPVLVFRGFG
                     SMGVVHSSEGNADFVGNFSQPNGAGFTRSWSTDVDTRIGAQANATINERLSAAVQVVA
                     QHRYDNSYSPQIEWANLKYQFTPEIGVRAGRTLLATFMESDSRLVSYSYPWVRPPQEI
                     YGMLPVTNKDGVDAFYRIQTGEATNTVQASYGDTILKTPGGGEVRGRPIFDINDTLEY
                     GAGTVRIGYTKLRGDVHSPTLDNLFGGLAQFGNTVSAIPGLQATGAQALALVSKYQLI
                     RFPYSVVTVGGSYDPGTWLLMGEWASAKTSAAISSANAWYVTGGYRFGDFMPYLTLAK
                     LKVDKLSEPGIQTAGLPPALAQTALALNSGLNAVINGYALSQQTVSMGLRWDFARSMA
                     LKLQYDRLSLGAGSTGWLGNIQPGFQPGGRVNVLSAVADFVF"
     gene            4497..4928
                     /locus_tag="VFW59_06180"
     CDS             4497..4928
                     /locus_tag="VFW59_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5363835.1"
                     /translation="MHLTKQIGLCVLGLALGIGANAAEADIVVVVSPSSPLLKMSKHQ
                     LADIYLGKASYFPDGKKAEPIDQPEGAAARDEFYAKFIGKSPVQVKAYWSKTIFTGRG
                     EPPREVESGIEVKNRVTENPYAIGYIEQNEVDDSVRVLLPQ"
     gene            complement(4971..7292)
                     /locus_tag="VFW59_06185"
     CDS             complement(4971..7292)
                     /locus_tag="VFW59_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5363836.1"
                     /translation="MHMSERWNAVGLWVRHALGRATEPHILFPAFAVLFLAAIWGMTA
                     NLIVVEHDAARHAAAASSRELVETYEAQVLRSLREIDQTLKIVKYAYEHKAATDALAE
                     LKSQALLPPDIVYEVSIANTQGTVIQSSRPVWMSNVSDQDFFMTQFFGNTISVGRPRK
                     NPDSGEWELVFSRRLGHADGSFAGVAMVTVDAAFFVSGYEPSKLGEHGVLGILGMDGI
                     FRARRTGDAVSAGETVDYTGATRGFDQPDSEAALTVNPWDGVRRYTGARQLYDFPLTV
                     IVGLSEDEQLAEVDRNMRVYFWRAVAGSTLLLLVVGALGRLSWQLAQTRLRAIEEQAA
                     HAKRIEHLAYHDVLTSLPNRSMFSTFLHQGINQARRHNKQLSVLFLDLDHFKPINDTL
                     GHEAGDQLLREVAERLKSCLRDSDTVARLGGDEFVVLLPELDEEKYQTTVAKKILASI
                     AKPFVLLGQEFRVTASIGISTYPQDGLDEQTLTKNADIAMYHAKEHGKNNFQFYSENL
                     DANSLERRTLESSLRHALEHQEFQLHYQAQRDIRSGRITGIEALLRWQRPDLGMEAPM
                     QFIPVAEETGLIVPIGKWVLKTACQQNMAWQSQGLPRLPMSINLSARQFFDETLLTNI
                     KATLRESGMQPSLLELGITENLLMRDVQKTLRVLTALRDMGVLIAIDNFGAVHSSLAT
                     LKQFPLDTIKIDQTYIHDVASGADKSRLTQAIVVLGHALGKTVVAQGVETREQAELLR
                     EHACDEFNGFYFNRPVPPEQFAELLRERGPVMK"
BASE COUNT         1481 a         2274 c         2453 g         1541 t
ORIGIN      
        1 tacaacatcc catatacctg ccagcagcca tgacgacctg ttaaaatgca cgggtttttc
       61 agtcaacata tctccccatg caagacaata aagattcaat gaccccggtg gaacgccggg
      121 ccagctttgg tttggcgggc atttacggcc tgcgcatgct ggggctgttc atcatcctgc
      181 cgatcttcgc tttgtatgcc gagcatctgc ccggcggcga gagccatatg ctgatcggca
      241 tcgcgctcgg cgcttacggc ctgacgcagg ccatcctgca gattccagcc ggttggctgt
      301 ccgaccgcta cgggcgcaag ccggtgatct acgccgggct ggccctgttc gctatcggca
      361 gctttatcgc ggcttcggcg gacgacatct actggatcat cgccgggcgg gtggtgcagg
      421 gcgcgggcgc gctgaacgcg gcggtgatgg cgctgactgc cgacctgacc cgcgaggagc
      481 accgcaccaa ggcgatggcg atgataggca tgactatcgg tattaccttt tcgctgtcgc
      541 tgatcctgtc gccgctgctc aacagcgtgc tcggcgtgcc gggcatcttt gcgctgaccg
      601 gcgcgctggc gattgcggcg atgctggtgg tgaaattcgt gattcccgat ccggcgatca
      661 cgcgcttcca caccgatacc gaggccgggc acgggcgctt cggcgaggtg ttgcgcaaca
      721 aggatctgct gcgcctggat ttcggcatct tctcgctgca cgcgatcctg atgtcggtgt
      781 tcatgcaggt gccgttcgtg cttcaggccg acgggctgga tgtcgcgcag cattggcaga
      841 tttacctgcc ggtgatgctg atcgcattcg tgctgatggt tccgccgatc atcatcgccg
      901 agaaaaaggc caagatgaag caggtgttca tgggcgcggt ggcgctggcg ctggtcgcgc
      961 aggcgctgct gatgttcgcg cagcacagcc tgtggggcgt ggccgccgcg ttgctgatat
     1021 tcttcaccgc gttcaacgtg ctggaagcca cgctgccgtc gctgatcagc aagatcgcgc
     1081 cgctggcatc caaaggcacg gcgatgggcg tgtacagcag cgtgcagttt ctcggcgcgt
     1141 tcttcggcgc ggcggcgggc ggcgcgctga tgcagttcgt cggccacgat gcggtgttct
     1201 ggttcagcat cgtgctgctg gcgctgtggc tggtcgtcgc atccggcatg cgtccgcccg
     1261 ctgcggtgcg cacccggatg tatcatctgg ccgagatgac cgaggcggaa ggcgcgcaat
     1321 tgcagcaacg gctcgcgcaa ctgcaaggcg tgcgcgaagc gctggtggtg gcggcggaag
     1381 gcatggcctg cctcaaggtg gaaatgcagg gatttgatga agcagcagtc gaacaactcg
     1441 tgataaaagg agcatgacat gtcggtgaat aaagtgatct tggtgggccg tctgggcaag
     1501 gacccggaga cccgttacat gaccaacggt gaggcggtga ccaatgtctc gctggcgaca
     1561 tcggaaaatt acaaggacaa gagcggcgag aagcaggagc gtaccgagtg gcacaatctg
     1621 gtgttctacc gccgtctggc cgaagtggcc ggcgaatatc tgaagaaggg ttcgcaagtt
     1681 tacgtcgaag gccgcatcca gacccgcaag tggcaggaca aggaaggcaa ggagcgctac
     1741 accaccgaaa tcatcgtcaa cgaaatgcag atgctgggcg gcaagtccac cggcggcagc
     1801 ttcgaagtga tggaccagtc cgcgccggag cgttctgcgc ccgagcgctc cgcaccggca
     1861 cgctccgccg ccccggcagc cagacaggct ccggccccgg ccaagagcag cttcgataat
     1921 ttcgacgacg atattccgtt ctgaggttat cttgcgaaca caaaaaagcc cctgtttcag
     1981 gggctttttt ttgcgcccgg cagggcgaaa tcggcgtgct gcccgcgtat caggctgcgg
     2041 acagtgccgt ctgcatcttg cttaacgcct tctgctcgat ctggcggatg cgttcggcgg
     2101 atacattgaa ttcggcagcc agttcatgca gcgtcgcggc atttccctcg gacagccagc
     2161 gggcttcgac aatccggcgg ctgcggccat cgagattggc gagggcattc gcgaggccgg
     2221 tcgtttgcaa atgttcgcgt tcagccgttt cgaggatgtg cgaaggttca tgttccgcgt
     2281 cgtcggacag gtactggatc ggagagtagc tctcatcgtc gctgtcacct gtcggttcga
     2341 gcgcaacttc atgccccgcg aagcgcgatt ccatttccac gacatcgtgt tcgctgacgt
     2401 tgagctggcg ggcgatttcg ctgatctgct gcggcttgag cggctccagc ccctgcttca
     2461 tgctgcgcag gttgaagaac agcttgcgtt gcgctttggt ggtggcgatc ttgaccagac
     2521 gccagttacg cacgacgaac tcgtggattt cggcgcgtat ccagtgcagc gcaaacgaaa
     2581 ccagacgtac gccgcgtgcg gggtcataac gcttgaccgc cttcatcagg ccgatattcc
     2641 cctcctggat caagtctgcc tgcggcaggc cgtatccggc ataaccgcgc gcgacgctga
     2701 ccaccacgcg caagtgcgat agcaccaact ggcgcgcggc atccagatcg ttttcagtct
     2761 tgaagcgtgt cgcataagcg aattcttctt cctgactcag cacggggaaa cggttcaccg
     2821 actggatata agcctccagg ctgccgacgg tactgggtac aggaaaacaa atggttgcat
     2881 tcatggttac cctccttttg tctgatttta gcactcgtgg agtgagagtg ccaagctgcc
     2941 aaattgttcg gtgagcgttg cgggcaacca ggcaagaaac aataaaaaac cgggaagagc
     3001 gcaggatagg agcgattccc gggcaagtaa atgcatagat tcctgtatgt ctttacatgc
     3061 ctcaccctag tgtttcgtcc ggcggcggaa aagataattg ccgcataaat ctgggcaagc
     3121 cccatatgca ccgaataaat cgatgcgccg aataaatcgc agcaggcacg gaggcaagtc
     3181 atggtggtaa acagcgtcga atgcatgccg tacaaactga ccgccatcgc aacgctggtg
     3241 gcgatgctgt acgcgccttg cgcgcgcagc gaggagccgg acgcgcctgt tctggtattc
     3301 aggggattcg gttcgatggg tgtcgtgcat tcgagcgaag ggaatgccga ttttgtcggc
     3361 aatttttcgc agccgaacgg ggcgggcttc acccgaagct ggagtaccga cgtggatacc
     3421 cgcatcggcg cgcaggcgaa cgccactatc aatgaacgtc tgtcggcggc ggtgcaagtt
     3481 gtcgcgcagc atcgctacga caacagctat tcgccgcaaa tcgaatgggc caacctgaaa
     3541 tatcaattca cgccggagat cggcgtcagg gccggacgca cgctgctggc cacgttcatg
     3601 gaatccgact cgcggctggt gagttacagc tatccgtggg tgcggccgcc acaggagata
     3661 tacggaatgc tgcccgtgac aaacaaggat ggcgtcgatg cgttctaccg cattcaaacc
     3721 ggagaggcga ccaatacggt gcaggcctct tacggcgata cgatattgaa gacgccgggc
     3781 ggcggggagg tcaggggacg gcctattttc gacattaacg acacgctgga gtacggcgcg
     3841 ggcaccgttc gcatcggcta taccaagctg cgcggcgatg tgcatagccc gacgctggac
     3901 aacctgtttg gcggactcgc gcaattcggt aatacggtat cggcgatacc cggcttgcag
     3961 gcgaccggcg cgcaggcgct ggcgctcgtc agcaaatacc agttgatccg cttcccatat
     4021 tcggtggtga cggtaggcgg gagctacgat ccgggaacct ggctgctgat gggcgaatgg
     4081 gccagcgcga aaacctccgc cgcgatttcc agcgccaatg cgtggtatgt cacgggcgga
     4141 taccggttcg gcgatttcat gccctacctg acactggcaa aactcaaggt ggacaagctg
     4201 tccgaacccg gcatccagac ggcgggattg ccgcccgcgc tggcgcagac cgcgctggcg
     4261 ctcaacagcg ggctgaatgc ggtaatcaac ggctacgcgc tgtcccagca aaccgtgtcg
     4321 atggggctgc gctgggattt cgcgcggagc atggcgctca agctgcaata cgaccgcctg
     4381 agtctgggcg cggggtctac gggatggctg ggcaacattc agccgggatt tcagccgggc
     4441 ggcagagtca acgtgctcag cgccgttgcc gatttcgtat tctgacgaga tcagatatgc
     4501 acctcaccaa acaaatcgga ttatgcgtct tggggcttgc gctgggcatc ggggccaatg
     4561 cggcggaggc cgacatcgtg gtggtggtct cgccgagcag cccgctgctc aaaatgagca
     4621 agcatcaact ggcggatatt tatctgggca aggcgagcta tttcccggac ggcaagaaag
     4681 ccgagcctat cgatcagccg gaaggcgctg ccgcgcggga cgagttctac gcaaaattta
     4741 tcgggaaatc gccggtgcag gtaaaggcct attggtccaa gaccattttt accggcaggg
     4801 gtgagccgcc cagagaagtc gaaagcggca tcgaagtcaa aaatcgcgtc acggaaaatc
     4861 cctacgccat cggctacatc gagcagaacg aagtggacga ttcggtcagg gtgctgctgc
     4921 cgcagtaaag cgcctgcgcg tcggtccggc agcggcgcgc cgttcaggcg ctacttcatc
     4981 acggggccgc gctcccgcag caattccgca aactgctcgg gcggtacggg gcggttgaaa
     5041 tagaacccgt tgaactcgtc gcaggcatgc tcgcgtagca gctcggcctg ctccctcgtc
     5101 tccacgcctt gcgcgaccac cgttttgccc agcgcgtggc ccagcacgac aatggcctgg
     5161 gtcaggcggc tcttgtccgc gccgctggcg acatcgtgga tatacgtctg gtcgatcttg
     5221 atggtgtcga gcgggaattg cttgagcgtg gcgagcgacg aatgcacggc gccaaagttg
     5281 tcgatggcga tcagcacgcc catatcgcgc agcgcggtca gcacgcgcag ggttttttgc
     5341 acgtcgcgca tcagcaggtt ttcggtgatg ccgagttcca gcagcgatgg ctgcatgccg
     5401 ctttcgcgga gcgtcgcctt gatgttggtc agcaatgtct cgtcaaaaaa ctggcgggcg
     5461 ctcaggttga tggacatggg caggcgcggc agcccctgac tctgccatgc catgttttgc
     5521 tggcaagcgg tcttcagcac ccatttgccg atcggcacga tcaggcccgt ctcttccgcg
     5581 accgggatga actgcatcgg ggcctccatg ccgagatcgg gccgctgcca gcgcagcagc
     5641 gcctcgatgc cggtgatcct gccgctgcgg atgtcgcgct gggcctgata gtggagctgg
     5701 aattcctgat gctccagcgc gtggcgcaga ctcgattcca gcgtgcgccg ctccagcgag
     5761 ttcgcatcga gattttcgga atagaactgg aagttgtttt tgccgtgctc cttggcgtgg
     5821 tacatcgcga tgtccgcgtt tttcgtcagc gtctgctcgt ctagcccgtc ttgcggatag
     5881 gtgctgatgc cgatgctggc cgtgacgcga aactcctgcc cgagcagcac gaacggtttc
     5941 gcgatggagg cgaggatttt ttttgccacg gtggtctggt atttctcctc gtccagctcc
     6001 ggcagcagca ccacgaactc gtcgccgccc agccgggcga cggtatcgct gtcgcgcagg
     6061 caggatttga gccgctccgc cacctcgcgc agcaactggt cgccggcctc gtgtcccagc
     6121 gtgtcgttga tcggcttgaa atggtcgaga tcgaggaaca gcaccgacag ttgcttgttg
     6181 tggcggcgcg cctgattgat gccctgatgc aggaaggtgc tgaacatgct gcggttcggc
     6241 agcgacgtga gcacatcgtg ataggcgaga tgctcgatgc gcttcgcgtg ggccgcctgt
     6301 tcctcgatgg cgcgcagacg ggtctgggcg agctgccagc tcagccgtcc cagcgcgccg
     6361 accaccagca gcagcaacgt gctgcccgcg accgcgcgcc agaaatagac gcgcatgttc
     6421 cggtcgactt ccgcgagctg ctcgtcttcg gacaagccga cgatcacggt cagcggaaag
     6481 tcgtaaagct ggcgcgcgcc ggtgtagcgc cgcacgccgt cccacgggtt gaccgtcagc
     6541 gcagcctcgc tgtccggctg atcgaagccg cgtgtcgcgc cggtgtagtc aaccgtttct
     6601 ccggcagaca cggcatcgcc ggtgcgcctc gcccggaata tgccatccat cccgaggatg
     6661 ccgagcacac cgtgttcgcc cagtttcgac ggctcgtaac cgctgacgaa aaaggccgca
     6721 tcgaccgtca ccattgccac gccggcaaac gatccgtcgg cgtggccgag cctgcggctg
     6781 aataccagct cccactcgcc cgagtccgga ttcttgcgcg gacgtccgac cgaaatcgtg
     6841 ttgccgaaga actgcgtcat gaaaaaatcc tggtcggaga cattgctcat ccagaccggt
     6901 cgcgagcttt ggatgaccgt gccttgcgtg ttcgcgatac tgacctcata cacaatatcc
     6961 ggcggcaaca gcgcctggct cttgagttcc gccagcgcat ccgtagccgc tttgtgttcg
     7021 tatgcgtatt tgacgatctt gagcgtctgg tctatctcgc gcagcgagcg caatacctgc
     7081 gcctcgtagg tttcgaccag ctcgcggctg gatgcggcgg cggcatgtcg cgcggcatca
     7141 tgttcgacga cgatcaggtt ggcggtcatt ccccagatcg cggcgagaaa cagcaccgca
     7201 aacgccggga acaggatgtg cggctcggtc gcccgtccca gcgcatggcg cacccacagg
     7261 ccgaccgcat tccagcgctc gctcatatgc atggaattcc cctgccgccg ggccagcgcc
     7321 agcatgcccg ccgggggccg gggctatcgt gttgaagcgc gtataacatc ctgaaggctt
     7381 ccttatggct ggcttgcggg aatttacggc atgccgacga tccggattat tgcccgtcca
     7441 gggaagcggc acactggggt taaacccgca tacccggcga agtggctgcg cattttgacg
     7501 ggccgacgac gcgaatttcc ggccatgaag tggccggggt ggatgccggt atggaagggg
     7561 agtactgaac aagtccgttc gccctgagct tgtcgaaggg tgaactttgc gtgccgctga
     7621 tttattgctg tcctccgctc atggttcgac aagctgataa aacaactagc cattcgacta
     7681 ggcagtcaaa aaatgccagc caagtcgatg gttatcacca cgaacggagg acttgtttcg
     7741 cgtaggtta
//
LOCUS       DASUIV010000166         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_38790, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000166 DASUIV010000000
VERSION     DASUIV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38790"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFW59_06190"
     CDS             <1..760
                     /locus_tag="VFW59_06190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061391.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5363837.1"
                     /translation="VEKYVRSPRHVEIQVFADSHGAVTYLFERDCSLQRRHQKVIEEA
                     PAPGIDRKLIAKIGERCAEACRTIGYRGAGTFEFLFENGEFYFIEMNTRVQVEHPVTE
                     MITGIDIVQQQIRIAAGEKLPFRQRDIQFRGHAIECRINAEHPYKFTPSPGRITSWHV
                     PGGPGIRVDSHVYANYFVPPHYDSMIGKLIAYGDTREQAMARMRTALSEMAVEGIDTN
                     VPLHRELMQDAAFMNGGTSIHYLEERLAERSKGK"
     gene            760..1653
                     /gene="prmA"
                     /locus_tag="VFW59_06195"
     CDS             760..1653
                     /gene="prmA"
                     /locus_tag="VFW59_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX5363838.1"
                     /translation="MPWLTLIVDTDAAHAEALSEALLELGALSVDLLDADADTPDEQA
                     IFGEPGEPPPGVWQHNRVSALFDADKDVEAILRDAAADIGLKDLPEHRVETLDDNDWV
                     RLTQSQFEPIPISPRLWIVPTWHQPSDPSAINIVLDPGLAFGTGSHPTTRLCLRWLDG
                     NIKGGESVLDYGCGSGILAIAALKFGAASAVGIDVDAQAVSASRDNAIANQVENVRFA
                     LPDGTSGGQYDVVVANILTNPLRMLAPLLAGAAKQGGRIVLSGILEEQAQDVMDIYAQ
                     WFDLNAPVFEDGWSCLSGTKR"
     gene            1653..2651
                     /locus_tag="VFW59_06200"
     CDS             1653..2651
                     /locus_tag="VFW59_06200"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEX5363839.1"
                     /translation="MDGTTLCPHCDTRFRIAGTQLEAHQGMVRCGSCMQAFDARPGFI
                     PDQPDPQLELPILEDQVTATAEPTVAQVVQAPESPVSESPALELVEETPQAVTEIEFA
                     SDEAVEPVAIDAAPVSSDEAPVEPEHDGSLDFSRPADASADEMPSGKPLVIQPALLAG
                     SGPDEEAFSPPPRTRRWPWIIGLIVLLLLLCAQAAYFFRIELAARLPGMKPALVSYCK
                     LLNCSVPLPQNAELMSIESSELEADPEHDSRITLVALLRNRASHAQAFPHLELSLNDT
                     EDKVLARRTFKPADYLPATESEAVGLKPNHELNIRLKLDTTDLRPTGYRLALFYPQ"
BASE COUNT          517 a          885 c          833 g          499 t
ORIGIN      
        1 cgtggagaag tacgtccgtt cgccgcggca cgtcgagatc caggtctttg cggacagcca
       61 cggtgctgtg acctacctct tcgagcgcga ctgctcgctg cagcgccgcc accagaaggt
      121 gatcgaggaa gcccccgcgc ccggcatcga ccgcaagctg atcgcgaaga tcggcgaacg
      181 ctgcgccgaa gcctgccgca ccatcggcta ccgcggcgcc ggcaccttcg aattcctgtt
      241 cgagaacggt gagttctatt tcatcgaaat gaacacgcgt gtgcaggtcg agcatccggt
      301 caccgagatg atcaccggca tcgacatcgt gcagcagcag atccgcatcg cagcgggcga
      361 gaagctgccg ttccgtcagc gcgacatcca gttcaggggg catgccatcg agtgccgcat
      421 caacgccgag catccctaca agttcacccc gtcgccgggc cgcatcactt cctggcatgt
      481 gccgggcgga cccggcatcc gtgtcgattc gcatgtgtat gccaactatt tcgtgccgcc
      541 acattacgac tcgatgatcg gtaagctgat cgcttacggc gatacccgcg agcaggccat
      601 ggcgcggatg cgcaccgcgc tgtccgagat ggcggtggaa ggcatcgata ccaatgttcc
      661 gctgcatcgc gaattgatgc aggacgccgc cttcatgaac ggcggcacca gcatccatta
      721 tctggaagag cggctggcag aacgcagcaa aggcaagtaa tgccctggct gacgctgatc
      781 gttgacaccg atgccgcaca cgctgaggcg ctgagcgagg ctctgctcga actcggcgcg
      841 ttgtcggtgg acctgctgga tgccgatgcc gatacgccgg acgaacaggc gatctttggc
      901 gagcccggcg agccgccgcc cggcgtgtgg cagcacaacc gcgtcagcgc gctgttcgac
      961 gccgacaagg atgtcgaagc gatcctgcgc gatgcggctg ccgacatcgg actgaaagac
     1021 ctgcccgagc atcgcgtcga gacgctggac gacaacgact gggtgcgtct cacccagtcc
     1081 cagttcgaac ccatccctat ctctccgcgt ctgtggatcg tgccgacctg gcatcagcct
     1141 tccgacccga gcgcgatcaa tatcgtgctc gatccgggcc tcgccttcgg taccggcagc
     1201 catcccacca cgcggctctg tttgcgctgg ctggacggca acatcaaggg tggcgagagc
     1261 gtgctggact acggttgcgg ctccggcatc cttgccatcg ccgcgctcaa gttcggtgcg
     1321 gccagcgcgg tcggcatcga tgtcgatgcg caggcggtga gcgccagccg cgacaacgcc
     1381 atcgccaacc aggtcgagaa tgtgcgcttc gccttgccgg acggcacgtc tggcgggcaa
     1441 tatgacgtgg tcgtggcgaa catcctgacc aatccgttgc gcatgctcgc gccgctgctg
     1501 gcgggtgcag cgaagcaggg cggccgtatc gtgctgtccg gcatcctcga ggagcaggcg
     1561 caggacgtga tggatatcta tgcgcaatgg ttcgatctga acgcgccggt gttcgaggac
     1621 ggctggtcct gtctgtcggg aaccaagcgc tgatggacgg caccaccctc tgccctcatt
     1681 gcgatacccg ctttcgcata gccgggacgc agctcgaggc gcatcagggc atggtgcgtt
     1741 gcggcagctg catgcaagcc ttcgatgccc gccccggttt cattcccgat cagcccgacc
     1801 cgcaactgga actgccgata ctggaagatc aggtaacggc gacggccgag ccgacagtgg
     1861 cccaggtcgt gcaggcgcca gagtcgcccg tttccgaatc gcctgcactg gagctggtag
     1921 aagaaacacc gcaggcagtc accgagatcg agttcgcctc tgatgaagcg gtcgagcccg
     1981 ttgccatcga tgcggctcct gtctcgtcag atgaagcgcc tgtcgagccg gaacatgacg
     2041 gttcgctcga cttcagtcgg cctgccgacg cttctgccga tgagatgcca tccggcaagc
     2101 cgctggtgat ccagcccgcg ctgctggccg gtagcgggcc ggatgaagag gcgttttccc
     2161 ctccgccccg cacgcgccgt tggccatgga tcatcggtct gattgtgttg ttgttattgt
     2221 tgtgcgcaca agcggcttat ttcttccgca tcgaactcgc cgcgcgtctg cccggcatga
     2281 aacccgcgct ggtttcctac tgcaagctgc tcaattgcag cgttccgctg ccgcagaatg
     2341 ccgagctgat gagcatcgag tcctccgagc tggaagccga cccggaacat gacagccgga
     2401 tcacgctggt cgccttgctg cgcaaccgcg ccagccatgc gcaagcgttt ccgcatctgg
     2461 agctctcgct caacgatacc gaagacaagg tgctggcccg ccgtacattc aagccggccg
     2521 actacctgcc cgccaccgaa agtgaagctg tcggcttgaa gccgaaccac gaactcaaca
     2581 tccgcctgaa gctggatact accgatctca ggccgacggg gtataggctg gcgctgttct
     2641 atccgcaata acgctcttac tctattcaga gagcgggttt aaacaccatc aatccataca
     2701 cgatcagcat cgaaggaaat gccagcatgc cgag
//
LOCUS       DASUIV010000167         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_412153, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000167 DASUIV010000000
VERSION     DASUIV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412153"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VFW59_06205"
     CDS             <1..1396
                     /locus_tag="VFW59_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX5363840.1"
                     /translation="KGPDYIDPMWLGQAAGRPCRNLDLYLMERDDIVATFAKHAAEVN
                     LVEGNKGLYDGLALDGSNSNAALAKMLDLPVMLVIDARGMTRGIAPLILGYQAFDRDI
                     NFAGVILNNLGGSRHESKLRAVIEHYTDLPVIGAIHHDERLSIEERHLGLMPSNESHI
                     ASAKIKQIGEAIAEQVDLDRLLALSQKAPLAIPHSAPLPNPLPQAGEGAIVKSNFQFP
                     DKVRIAIARDCSFGFYYADDLDALEAAGAELVPFDALRDAQLPEVDALYIGGGFPETC
                     AAELEANTSLRGEIRQAIENGMPAYAECGGLMYLSRGIEYQGRTYEMAGAIPGDVKMH
                     AKPIGRGYVHLKEDEAHPWPRPRPLSNPSPQSSPASGRGDEREKQPTIFAHEFHYSSL
                     ENLPEGSRFAYHVERGYGIDGDRDGLVVHNLLASYTHLRTIGSCYWATRFVAFIRRCK
                     HHQSEQTTISELAK"
     gene            1393..1992
                     /locus_tag="VFW59_06210"
     CDS             1393..1992
                     /locus_tag="VFW59_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HEX5363841.1"
                     /translation="MTEQLYQAAIASFDKANAEDPNKETADGQQYPKELLYARRMTEM
                     QERFAPEASEAVKLAVRAQHIQRWKIPRSDYAMDKPGYMLWRTTLYKFHAETAGKLMK
                     EVGYDDEMIERVKAIVSKKELKTNPETQMMEDIVDLVFIEHYMLAFAGQHADYDEAKW
                     ILIIKKTWNKMSQRAHEFALSGKIALPEALVPLILKAVQ"
     gene            1994..>2487
                     /locus_tag="VFW59_06215"
     CDS             1994..>2487
                     /locus_tag="VFW59_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX5363842.1"
                     /translation="MDFLPIFANVRNRLCLVVGGGSVGSRKAGVLLEAGAKVRVVAPE
                     IDEALKANNNVEAVVARFEPQHLDGVTLVIAATNDREVNKQVSELAQARNIPVNVVDE
                     PALCSFIMPAILDRSPLMVAFSSGGASPVLTRMMRGKLETVIPQNYSRLAAFAERFRE
                     LVKTR"
BASE COUNT          525 a          738 c          776 g          448 t
ORIGIN      
        1 gaagggcccg gattacatcg acccgatgtg gctgggtcag gccgccggac gcccctgccg
       61 caatctcgat ctgtatctga tggagcgcga cgatatcgtc gccactttcg caaagcacgc
      121 cgccgaagtg aatctggtcg agggcaacaa ggggttgtat gacggcctcg cgctggacgg
      181 cagcaacagc aacgccgcgc tggcgaagat gctggatttg ccggtgatgc tggtgatcga
      241 tgcgcgcggt atgacgcgcg gtatcgcacc gttgatcctc ggctatcagg cgttcgaccg
      301 cgacatcaat tttgccgggg tgatactcaa caatctcggc ggatcacgcc acgaatccaa
      361 gctgcgcgcg gtgatcgagc attacaccga cctgccggtg atcggcgcga ttcatcacga
      421 cgagcggctc agcatcgagg agcgtcatct cggcctgatg cccagcaacg agtcgcatat
      481 cgcgagcgcg aagatcaagc agatcggcga ggccatcgcg gaacaggtcg atctggaccg
      541 gttgctcgcg ctgtcgcaga aggcgccgct ggcaattccg cacagcgccc ctctccccaa
      601 ccctctcccg caagcgggcg agggagcaat cgtgaaaagc aattttcaat ttcctgacaa
      661 ggtacgcatc gccatcgcgc gcgactgttc cttcggtttc tactacgccg acgatcttga
      721 cgcgctggag gcggccgggg cagaactggt tccattcgac gcgctgcgcg atgcacagtt
      781 gccggaagtg gatgcgctgt acatcggcgg cggttttccc gagacctgtg ccgccgagct
      841 ggaggcgaac acctcgttgc gcggcgagat caggcaggcg atcgagaacg gtatgccggc
      901 ctatgccgag tgcggcgggc tgatgtatct gtcgcgcggc atcgagtatc aggggcgcac
      961 ctatgaaatg gccggcgcga ttcccggcga cgtgaaaatg cacgccaaac ccattggtcg
     1021 cggctatgtt catctcaagg aagacgaggc gcatccgtgg ccgcgccccc gcccgctttc
     1081 gaacccctct cctcaatcct ctcccgcaag cgggcgagga gacgaacgag aaaaacaacc
     1141 gacaattttt gcgcacgagt tccattactc cagtctggaa aatcttccgg agggcagtcg
     1201 cttcgcttac catgtcgagc gcggttacgg catagacggg gacagggacg gtctcgtcgt
     1261 gcataacctg ctcgcttcgt acacccatct gcgcactatc ggcagttgct actgggcgac
     1321 acgttttgtg gcgttcatcc ggcgctgcaa gcatcatcaa tctgaacaga caacaatcag
     1381 cgagctcgca aaatgacaga acagctatac caggcagcca ttgcctcctt tgacaaggcc
     1441 aatgccgaag atccgaacaa ggaaacggcg gacggccagc agtatcccaa ggagctgctc
     1501 tatgcgcggc gcatgaccga gatgcaggag cgtttcgcac ccgaagcctc ggaagcggtg
     1561 aagctggcag tgcgcgccca gcatatccag cgctggaaga ttccgcgcag cgactatgcg
     1621 atggacaagc ccggctacat gctgtggcgc accacgctgt acaaattcca cgcagaaacg
     1681 gccggaaagc tgatgaagga agtgggctac gacgacgaga tgatcgagcg tgtcaaagcc
     1741 atcgtcagca agaaagagct caagaccaat cccgagacgc agatgatgga agacatcgtc
     1801 gatctggtgt tcatcgagca ctacatgctg gcgtttgccg ggcaacatgc cgactacgat
     1861 gaggcgaagt ggatactcat catcaagaag acctggaaca agatgtcgca gcgcgcgcat
     1921 gagttcgcgc tgtcgggcaa gatcgcgttg ccggaggcgc tggtgccgct gatcctgaag
     1981 gcggtgcaat agcatggact tcctgccgat ctttgcgaat gtccggaaca ggctttgcct
     2041 cgtggtcggc ggcggttcgg tcggttcgcg caaggcgggc gtgctgctcg aagccggtgc
     2101 gaaggtgcgc gtggtcgcgc cggagattga cgaagcgctg aaggcgaaca acaacgtcga
     2161 ggctgtcgtc gcccgtttcg agccgcagca tctcgacggc gtgacgctgg tcatcgccgc
     2221 gaccaacgac cgggaggtga acaagcaggt ctccgaattg gcacaggcgc gcaacatccc
     2281 ggtcaatgtg gttgacgagc ccgcgctgtg ctctttcatc atgcccgcga tactcgaccg
     2341 ttcgccgctg atggtggcgt tctccagcgg aggtgcatcg ccggtgctga cgcgcatgat
     2401 gcgcggcaag ctggagaccg tcattccgca gaactacagc cgtctggccg cctttgccga
     2461 gcgcttccgc gaactggtga agacgcg
//
LOCUS       DASUIV010000168        10643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1341721, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000168 DASUIV010000000
VERSION     DASUIV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10643
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341721"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2683
                     /gene="gcvP"
                     /locus_tag="VFW59_06220"
     CDS             <1..2683
                     /gene="gcvP"
                     /locus_tag="VFW59_06220"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX5363843.1"
                     /translation="EYAYVEHLRGIAAKNRLYKSYIGLGYYNTILPAVIQRNVLENPG
                     WYTAYTPYQAEIAQGRLEALLNFQTMIVDLTGMEIANASLLDEATAAAEAMTMLHGLR
                     PRDAADKNSFFVSHECFPQTIELLKTRAKPLGIELVIGDFKTVTLNDKLYGALLQYPT
                     ADGTVHDYADFVARAKANGMTIAVAADILSLVLLTPPGEWGADVVLGSTQRFGVPMGY
                     GGPHAAYFACRDAHKRSMPGRIIGVSVDADGNPALRMALQTREQHIRREKATSNICTA
                     QALLAIMAGMYAVYHGADGLRGIAAQVHRSTALLAAELKKLGYTVAEGVFFDTLKLMH
                     TDNVKVHALAEAAQINFRYTVDGLSISLDQTTSVEDLNAILAVFAQKAGKTAPLLTAA
                     QLSAQALLVKPRQSAILSHPVFNAYRSETEMMRYIKRLENKDLSLTHSMISLGSCTMK
                     LNAASEMLALTWPEFANLHPFVPLEQVEGFQELIAGLDAALSEITGFAQMSFQPNSGA
                     SGEYAGLLVIQAYHRSRGEAQRNVVLIPSSAHGTNPASAAMCGMHIVVVKCDARGNID
                     VDDLRAKAEEHKADLSCLMVTYPSTHGVYEESIKDITAIIHANGGQVYMDGANMNAQV
                     GLTSPGNIGADVCHLNLHKTFAIPHGGGGPGAGPIGVAEHLTPFLPSHPVVKVGGAQG
                     IHAVSAAPYGSALILLISYGYIKMMGGAGLTEATKMAILNANYIKESLKDHYATLYSG
                     ANGRCAHEMILDCRDWKKDGVEVADIAKRLMDFGFHAPTTSFPVVDTLMVEPTESESK
                     AELDRFVGAMIAIRKEVEEVVSGRVDKKDNILKHAPHTAKSVCANEWARPYSREQAAY
                     PLSWVRDNKFWPAVARVDNVYGDKNLICACPPTESYT"
     gene            2694..3215
                     /gene="tpx"
                     /locus_tag="VFW59_06225"
     CDS             2694..3215
                     /gene="tpx"
                     /locus_tag="VFW59_06225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX5363844.1"
                     /translation="MSRNTADKTAVTLGGAPVTLYGTFPVVGQVAPAFTLVDKDLKDV
                     SLQSYAGKRKVLNIVPSLDTAVCATSARKFNEAASKLDNTVVLVISADLPFAMSRFCA
                     AEGLDNVTTLSLMRGRDFMRNYGVKIADTVLSGVTARAVLVLDENDRVLHAELVSDIT
                     HEPNYEAALAALG"
     gene            3272..5998
                     /locus_tag="VFW59_06230"
     CDS             3272..5998
                     /locus_tag="VFW59_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX5363845.1"
                     /translation="MEMVHTGTHAQVSANNNWHSLPAQDVLAALDAVSDGLSSDDAQR
                     RLEQYGPNRLTPPSRRGPLLRFLLQFHNLLIYVLLASAAVTALLAHWVDTAVIVGVVV
                     INAIIGFIQEGKAERAMDAIRRMLSPEATVLREGQRRVVEAGALVPGDIVLLQSGDKV
                     PADLRLLNAKNLRIEEAALTGESAAVEKNAVPVDAQAVPGDRYCMAYSSTLVVYGQGK
                     GVVVATADETEIGRISAMLEEVETLTTPLLRQMETFGRWLTVVIMALAVVCFLFGWLV
                     HSFGFGDMFLAAVSMAVAAIPEGLPAVLTITLALGVQRMAKRSAIVRRLPAVEALGSV
                     TTICTDKTGTLTRNEMTVQRVITAAQVFEVSGTGYAPHGGFSSDGIETGMTEQALDLL
                     RAGLLCNDAQLHENVGVWHVAGDPTEGALIPLALKAGMDAQFEQGAMPRTDHIPFESE
                     HRFMATLHHDHAGRALVYVKGAVEQVLAMCNKQRGAGEDEALNLPYWQERMAGSGALG
                     QRVLALAMKTVDGGQRELSFTDMAGGFTLLGMVGISDPPREEAIAAVRDCIAAGIRVK
                     MITGDHADTARAIAAQIGIGKGLALTGVEIDALDDEALRQAVREVDVFARASPAHKLR
                     LVTALQANGEIVAMTGDGVNDAPALKRADIGVAMGRKGTEVAKEAAEMVLADDNFATI
                     AHAVEEGRTVYDNIKKAIVFIMPTNGGEAGIVLIAILFGMTLPITPVQILWVNMVSAV
                     TLALALSFEQAEPDVMRRPPRAAGEPILSGFMIWRISFVSVLLTLGTVALFQWALARG
                     VSLEAARTLAVNALVTGEITYLFNCRYLLAPVRSWQDFTGNRYVLLSIGVLILLQLAF
                     TYLPFMQRLFGVVALDAAAWVSILGFGVLLFVIVETEKLWLSRCAARAAGRR"
     gene            6067..6498
                     /locus_tag="VFW59_06235"
     CDS             6067..6498
                     /locus_tag="VFW59_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5363846.1"
                     /translation="MKILVAVDLADATEKVVNKVEELAPLLSAAVWLLHVAEPEPDFV
                     GLDVGPQHVRDALSNVFHHEHVQIQQIAERLRSRGIDTTALLVQGATAEGILQEAAKL
                     QVDMIVLGSHGSGAVRQIVLGSVSAGVLQKSACPVLLVPIR"
     gene            6654..8069
                     /gene="atpD"
                     /locus_tag="VFW59_06240"
     CDS             6654..8069
                     /gene="atpD"
                     /locus_tag="VFW59_06240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5363847.1"
                     /translation="MNLGTVVSVRGSVVDMRFEHRLPPIYSLLHAGTDDRISIEVLSQ
                     LDAHRVRGIALTPTQGLARGMQVTDTGGPLKTPVGKATLSRMFNVFGDTIDRQPELAD
                     VEWRSVHRAPPSLARRSTQSEVFETGIKIIDVLAPLERGGKAGLFGGAGVGKTVLLTE
                     MIHNMVGHQEGVSIFCGIGERCREGEELYRDMKEAGVLPNMVMVFGQMNEPPGSRFRV
                     GHAALTMAEYFRDDEHRDVLLLIDNIFRFIQAGMEVSGLMGQMPSRLGYQPTMGTELS
                     QLEERIANTDTGAITSIQAVYVPADDFTDPAAVHVFSHLSASIVLSRKRASEGFFPAV
                     DPLQSSSKMATPGIVGERHYAVAQEIRRTLAQYAELRDIIAMLGLEQLSVEDRNVVGR
                     ARRLERFLTQPFFTTEQFTGFKGTLVSLDDALDGCERILRDEFKDYPESALYMIGKID
                     EAIAKNKAGPEAEHAGVAHES"
     gene            8059..8448
                     /locus_tag="VFW59_06245"
     CDS             8059..8448
                     /locus_tag="VFW59_06245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX5363848.1"
                     /translation="MNLKVLLPYKVFAEKRGVSRIVARTGEGSFGLLPRRLDCVAALV
                     PGILTFETPEEGEVYIAVDEGILVKAGKDVLISVRNAIGGMDLDKLHAAVGREFLALD
                     DQEKMLRSVLAKLERGFIRRFAEFHHE"
     gene            8441..8776
                     /locus_tag="VFW59_06250"
     CDS             8441..8776
                     /locus_tag="VFW59_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX5363849.1"
                     /translation="MSEERHNKALPDETKFSRQVGEMASLKLKAQRHATQTVWAGLGM
                     MGLVGWSVAIPTLLGAALGIWIDEHYPGSHSWTLALLAIGLGLGCFNAWHWVSKEEKE
                     IHAEGGGHD"
     gene            8769..9071
                     /locus_tag="VFW59_06255"
     CDS             8769..9071
                     /locus_tag="VFW59_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX5363850.1"
                     /translation="MTDPLGLVSASAMGFLLGAFFFGGLWWTVRRGVASERVALWFFG
                     SMLLRTGVVMLGFYFLMGDSWQRLLAGLFGFVLARMAVTRLTGQAKVSAREAEHAP"
     gene            9061..9756
                     /locus_tag="VFW59_06260"
     CDS             9061..9756
                     /locus_tag="VFW59_06260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5363851.1"
                     /translation="MHLSPDEIIFWQSGLFKLNATIVYTWGLMAVLVIGSKLVTRKLS
                     MGLQRTRWQNLLEIVVSGIEKQIGEVGLRRPQKYIGFLGTLFLFVASASLFTIVPGYE
                     PPTGSLSTTAALAVCVFVAVPLFGIGASGLGEYLREYLQPTFIMLPFNIISELSRTLA
                     LAARLFGNMMSGTMILAILLTITPYVFPVALSMLGLLTGMVQAYIFSILAAVYIAAAT
                     RSGRPDSAVTDEK"
     gene            9781..10062
                     /locus_tag="VFW59_06265"
     CDS             9781..10062
                     /locus_tag="VFW59_06265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5363852.1"
                     /translation="MDSTTIIAVASIVTAGLTIAIGSIGPALGEGRAVSTALTSLAQQ
                     PDASATITRTLFVGLAMVESIAIYCFVVSMILLFANPFWNHAIAQAAGK"
     gene            10066..>10643
                     /locus_tag="VFW59_06270"
     CDS             10066..>10643
                     /locus_tag="VFW59_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5363853.1"
                     /translation="MLIDWFTVIAQVFNFLILVWLLKRFLYRPILDAIDAREKRIAAE
                     LADADAKKAEALKERDEFQRKNEAFDQQRAALLGRAMDEAKAERQRLLDEARKAADEL
                     SAKRQEILRSEAHNLNQALGSRARQEVFASARKTLADLASVSLEERMGEVFIRRLREM
                     DGKGKAGLGEALKSASSPGIVRSAFDLPAEQRA"
BASE COUNT         1980 a         3099 c         3538 g         2026 t
ORIGIN      
        1 agagtatgcc tatgtcgaac atctgcgcgg catcgcggcg aagaaccggc tttacaaatc
       61 ctatatcggc ttgggttatt acaacaccat cctgcctgcg gtgatccagc gcaacgtgct
      121 ggaaaatccg ggctggtaca ccgcttacac gccttatcag gcggagatcg cgcaggggcg
      181 tctggaagcg ctgctgaatt tccagacgat gattgtggat ttgaccggca tggagatcgc
      241 caatgcatcg ctgctggacg aggccaccgc agccgcagaa gcgatgacca tgctgcacgg
      301 cctgcgcccg cgcgacgcag ccgacaagaa cagcttcttc gtatcgcatg aatgtttccc
      361 gcagaccatc gagttgctga agacgcgtgc gaagccgctg ggcatcgaac tggtgatcgg
      421 cgacttcaag accgtcacgc tgaacgacaa gctgtacggc gcgctgctgc aatatccgac
      481 cgcagacggc acggtgcacg actacgccga tttcgtcgcg cgcgccaagg ctaacggcat
      541 gaccatcgcg gtggcggcgg acatcctgag tctggtgctg ctgacgccgc cgggcgaatg
      601 gggtgcggat gtggtgctgg gctccacgca gcgcttcggc gtgccgatgg gctacggcgg
      661 cccgcatgcg gcctatttcg cctgccgcga cgcgcacaag cgctcgatgc cggggcgcat
      721 catcggcgtg tcggtcgatg ccgacggtaa tccggcgttg cgcatggcgc tgcaaacccg
      781 cgagcagcat atccgccgcg agaaggcgac ttccaacatc tgcaccgcgc aggcactgct
      841 ggcgattatg gcgggcatgt atgcggtgta ccacggcgcg gacggtctgc gcggcatcgc
      901 cgcacaggtg catcgctcca ctgcattgct ggcagctgag ttgaagaagc tcggctacac
      961 cgtcgccgaa ggcgtgttct tcgacacgct gaaactcatg cataccgaca acgtgaaggt
     1021 gcatgcgctg gcggaagcgg cgcagatcaa cttccgttat accgtcgacg gcctgagcat
     1081 ctcgctggac cagaccactt cggtcgaaga cctcaacgcc atcctcgcgg tgttcgcgca
     1141 aaaagcgggc aagacagcgc cgctgctcac tgcggcgcag ctatccgcgc aagcgctgct
     1201 cgtgaagccg cgccagtccg ccattctgag ccatccggtg ttcaatgctt accgttccga
     1261 gacggagatg atgcgttaca tcaagcggct ggagaacaag gatctgtcgc tgacgcattc
     1321 gatgatctcg ctgggttcct gcaccatgaa gctgaacgcg gcctcggaga tgctggcgct
     1381 gacctggccg gaattcgcca acctgcatcc cttcgtgccg ctggaacagg tggaaggttt
     1441 tcaggagctg atcgccggac tggacgcggc gctgtccgag atcaccggct tcgcgcagat
     1501 gagcttccag cccaacagcg gcgcgtccgg cgaatacgcg ggcctgctgg tgatacaggc
     1561 ctaccaccgt tcgcgcggcg aagcgcagcg caacgtggtg ctgatcccct cctccgcgca
     1621 tggcactaat cctgccagtg cggcgatgtg cggcatgcac atcgtggtgg tgaaatgcga
     1681 tgcacgcggc aacatcgacg tggacgatct gcgcgcgaag gccgaggagc acaaggccga
     1741 cctgtcctgt ctgatggtga cttaccccag cacgcacggc gtatatgaag agtccatcaa
     1801 ggacatcacc gcgatcatcc acgcgaacgg cggccaggtg tacatggacg gcgcgaacat
     1861 gaatgcgcag gtcggcctga ccagccccgg caacatcggc gcggacgtgt gccacctgaa
     1921 cctgcacaag actttcgcga tccctcacgg cggcggcggg ccgggcgccg gcccgatcgg
     1981 cgtggcggag catctcaccc cgttcctgcc gtcgcatccg gtagtcaagg tcggcggcgc
     2041 gcagggtatc cacgcggtgt ctgcggcgcc ttacggcagc gcgctgatcc tgctgatctc
     2101 ctacggctac atcaagatga tgggcggtgc ggggctcacc gaggcgacca agatggcgat
     2161 cctgaacgcg aactacatca aggaatcgtt gaaggaccat tacgccacgc tgtacagcgg
     2221 agctaacggc cgttgcgcgc acgagatgat cctcgattgc cgcgactgga aaaaagacgg
     2281 cgtggaagtg gccgacatcg ccaagcggct gatggacttc ggcttccacg cgccgaccac
     2341 ttcgttcccg gtggtggaca cgctgatggt cgagccgacc gagagcgaat cgaaagccga
     2401 gctggaccgt ttcgttggcg cgatgatcgc catccgcaag gaggtcgagg aggtggtttc
     2461 cggccgtgtc gacaagaagg acaacattct caagcatgcg ccgcataccg cgaagtcggt
     2521 gtgcgccaac gagtgggcgc gtccttactc gcgcgaacaa gccgcgtatc cgctatcatg
     2581 ggtgcgtgac aacaagttct ggcctgccgt cgcacgcgtc gacaacgtat acggtgacaa
     2641 gaacctgatc tgcgcctgtc cacccaccga gtcgtatacc tgaggagagc tgaatgtcca
     2701 gaaacaccgc cgataagact gctgtaacgt taggcggtgc tcccgtcacc ctgtacggaa
     2761 ccttccctgt cgtgggccag gtcgcacccg cattcacgct ggtggataaa gacctgaagg
     2821 acgtgtcgct gcaaagctat gcgggcaagc gcaaggtgct gaacatcgtg ccgagtctgg
     2881 ataccgccgt gtgtgccacc tctgcccgca agttcaacga ggccgccagc aagctggaca
     2941 acaccgtggt actggtgatc tccgccgacc tgccgttcgc catgagccgc ttctgcgcgg
     3001 ccgaagggtt ggacaatgtg acgaccctgt cgctgatgcg agggcgcgac ttcatgcgca
     3061 attacggcgt gaagatcgcg gatacggtgc tgtccggtgt caccgcccgt gccgtgctgg
     3121 tgctggatga gaacgatcgc gtgctgcatg ccgagctggt cagcgacatc acccatgagc
     3181 cgaactatga ggcggcgctg gctgcgctgg gctgagcggc gtcaacgcgg tggtaacatg
     3241 gaggcccgtg ctcgtctgat ttgaaagggt gatggaaatg gtgcataccg gcacgcatgc
     3301 ccaagtttcc gcaaacaaca actggcacag tctgcctgct caggatgtgc tggccgcact
     3361 cgatgcggtg tcggacggtc tctcatccga cgacgcccaa cggcgtctgg aacaatacgg
     3421 tccgaaccgg ctgacgccgc ccagccgacg cggaccgctg ctgcgcttcc tgctgcaatt
     3481 ccataacctg ctgatctatg tgctgctggc ttcggcggca gtcacggcct tgctcgcgca
     3541 ttgggtggat accgcagtga tcgtcggtgt ggtggtgatc aacgcgatca tcggtttcat
     3601 tcaggagggc aaggcggaac gcgcgatgga tgcgatccgc cgcatgctgt ctcccgaggc
     3661 cacggtgttg cgtgagggcc agcgcagggt ggtggaagcc ggggcgctgg tgcccggcga
     3721 catcgtgttg ctgcagtccg gcgacaaggt ccccgccgac ctgcgcctgc tgaacgcgaa
     3781 gaacctgcgc atcgaggaag cggcgctgac cggcgaatcc gccgccgtgg aaaaaaatgc
     3841 cgtccccgtg gacgcgcaag ccgtgccggg cgaccgctac tgtatggcct attccagcac
     3901 gctggtggtg tacggacagg gcaagggcgt ggtggtggcg accgccgacg agaccgagat
     3961 cgggcgcatc agcgcgatgc tggaagaggt cgagacgctg accactccgc tgctgcggca
     4021 gatggaaacg ttcgggcgct ggctgacggt cgtcatcatg gcgctggccg tggtctgttt
     4081 tctgttcggc tggttggtac acagcttcgg cttcggcgac atgttcctcg cggcggtgag
     4141 catggcggtg gcggccatcc cggaaggctt gcctgcggtg ctgaccatca cgctcgcatt
     4201 gggcgtgcag cgcatggcaa agcgcagcgc catcgtgcgg cggttgccgg cggtcgaagc
     4261 gctgggttca gtcaccacca tctgcaccga caagaccggc acgctgaccc gcaacgagat
     4321 gacggtgcag cgcgtaatca ccgcagcaca ggtgttcgag gtgagcggga caggctatgc
     4381 gccgcacggc ggtttttcgt cagacggcat cgagaccggc atgacggaac aggcactcga
     4441 cctgctgcgt gccggtctgc tctgcaacga cgcgcagctg catgaaaacg tcggcgtctg
     4501 gcacgtcgcg ggcgatccga ccgagggcgc gctgatcccg ctggcgctga aggccgggat
     4561 ggatgcgcaa ttcgagcagg gtgcaatgcc gcgcaccgac cacatccctt tcgaatccga
     4621 acatcgtttc atggcgacgc tgcatcacga tcatgcgggc cgtgccctcg tctacgtgaa
     4681 aggggcggtg gaacaggtgc tggcgatgtg caacaagcag cgaggcgcgg gcgaagacga
     4741 ggcactgaac ctgccgtact ggcaagaacg catggccggg agcggcgcgc tcggccagcg
     4801 cgtgctggcg ctggcgatga agacggtgga tggcggccag cgcgaactga gtttcaccga
     4861 catggcaggc ggctttacgc tgctgggcat ggtcggcatc agcgacccgc cgcgcgagga
     4921 ggccatcgcc gcagtgcgcg attgcattgc tgctggcatc cgcgtcaaga tgattaccgg
     4981 agaccatgcc gataccgcgc gcgcgattgc cgctcagatc ggcataggca aggggcttgc
     5041 gctgacaggc gtcgaaatcg acgcgctgga cgacgaggct ctgcgtcagg cggtgcgcga
     5101 ggtggatgtg ttcgcgcgcg cgagtcccgc gcacaagctg cgcctcgtca cggccttgca
     5161 ggccaacggc gagatcgtcg cgatgaccgg cgacggggtg aacgatgcgc ccgcgctgaa
     5221 gcgcgccgac atcggcgtgg cgatgggcag aaaaggcacg gaagtcgcca aggaagcggc
     5281 ggagatggtg ctggccgacg acaacttcgc gaccatcgcg cacgcggtcg aagagggccg
     5341 caccgtctac gacaacatca agaaggctat cgtgttcatc atgccgacca atggcggcga
     5401 ggccggcatc gtgctgatcg cgatcctgtt tggcatgacg ctgccgatca cgccggtgca
     5461 gattctgtgg gtgaacatgg tgagcgcggt gacgctggcg ctggcgctgt cgttcgagca
     5521 ggccgagccg gacgtgatgc gccgtccgcc gcgcgccgct ggcgagccca tcctgtccgg
     5581 cttcatgatc tggcgcatca gctttgtctc ggtgctgctc acgctcggga cggtcgcgct
     5641 gttccagtgg gcgcttgcgc gcggcgtcag tctggaggcg gcccgcacgt tggcggtcaa
     5701 cgcgctggtg acgggcgaaa tcacttacct gttcaattgc cgctacctgc tcgcgccggt
     5761 gcgcagctgg caggatttca ccggcaaccg ctatgtgctg ctcagcatag gcgtgctgat
     5821 ccttttgcag ctcgcattca cctatttgcc gttcatgcag cggctgttcg gtgtggtcgc
     5881 gctggatgcg gctgcatggg tatcgatcct cggcttcggc gtgctgctgt tcgtcatcgt
     5941 cgagaccgaa aagttgtggc tcagtcgatg cgccgcgcgt gctgccggcc gcaggtaagt
     6001 tcgtgcggca acggagagcc cggatcgttt tcagcttggc ggatgcacaa tgaagggagg
     6061 gaagaaatga aaattctggt ggcggttgac cttgccgatg cgacggagaa ggtggtgaat
     6121 aaggtcgaag agctggcgcc gttgctgtcg gcggcggtgt ggctgcttca tgtggccgag
     6181 ccggagccgg attttgtcgg gctggacgtc ggcccgcagc atgtccggga cgccctgtcg
     6241 aacgtatttc accatgagca cgtccagata cagcagatcg ccgagcgctt gcgcagcagg
     6301 ggaatcgata cgacggccct gctggtgcag ggcgcgacgg cggaagggat tctgcaggaa
     6361 gccgcaaaac ttcaggtgga tatgatcgtc ctgggttcgc atggcagtgg cgcggtgcgg
     6421 cagatcgtgc tgggcagtgt cagcgccggg gtgctgcaaa aatcggcgtg cccggttctg
     6481 ctcgtgccga tccgctagca tcgctgccgt gacgttgctc ccgggaatgt ttcgcccggc
     6541 ttgcccgctt ccttcggcgc agccctgcaa aagatctggg tgtatgctat ggcccgattg
     6601 caagaatcca ttggagatag acgacaaaga atatggatag cggaagcgct tctttgaatc
     6661 tgggtacggt cgtttcggtg cgcggtagcg tcgtggatat gcgctttgag catcgtttgc
     6721 cgccgattta ctcattgctg catgcgggga ctgacgaccg gatctccatc gaggttctgt
     6781 cccagctcga tgcgcatcgg gtacgcggca tcgcactgac gccgactcag gggctggctc
     6841 ggggcatgca ggtaacagac accggaggcc cgttaaagac cccggtgggg aaggccacgc
     6901 tctcgcgcat gttcaatgtg ttcggcgata ccatcgaccg tcagccggag ttggccgatg
     6961 tcgaatggcg cagcgtgcat cgcgcccctc cttctctggc gcgccgctcc acccaatccg
     7021 aagtattcga aaccggcatc aagatcatcg atgtgctggc gccgctggag cgcggcggca
     7081 aagcggggct gttcggcggt gcgggtgtgg gcaagacggt gctgctgacg gaaatgatcc
     7141 acaacatggt cgggcatcag gagggggtca gtatcttttg cggcatcggc gaacgttgcc
     7201 gcgaaggcga ggaactttac cgcgacatga aagaggccgg ggtgttgccc aacatggtga
     7261 tggtgttcgg gcagatgaac gaaccgcccg gcagccggtt ccgggtgggg cacgccgcgc
     7321 tgacgatggc ggaatatttc cgcgatgacg aacaccgcga tgtgttgttg ctgatcgata
     7381 acatcttccg cttcatccag gcaggcatgg aggtctcggg tttgatgggg cagatgccct
     7441 cgcgtctcgg ttaccagccg acgatgggca ctgagttgtc gcagctggaa gagcgcatcg
     7501 ccaataccga caccggcgcg atcacctcga tccaagccgt gtatgtgccg gcggacgact
     7561 tcaccgatcc ggcggcggtg catgtgttct cgcatctatc cgcctccatc gtgctttcgc
     7621 gcaagcgcgc gagcgaaggt ttctttccgg ctgtcgatcc gttgcaatcc agttccaaga
     7681 tggctacccc gggtatcgtg ggcgaacggc actacgccgt ggcgcaggag atccgccgca
     7741 cgctggcgca atatgcggaa ctcagggata tcatcgcgat gctgggtctg gagcagcttt
     7801 cggtggagga ccgcaacgtg gtcgggcgtg cgcgccgtct ggagcgtttc ctgacccagc
     7861 ccttctttac cactgagcaa ttcaccggtt ttaaaggcac gctcgtcagc ctcgacgatg
     7921 cgctggatgg ctgtgagcgc atcctgcgcg atgaattcaa ggactatccc gagagtgcgc
     7981 tctacatgat cggcaagatc gatgaggcga ttgcaaagaa caaggccggg ccagaggcgg
     8041 aacatgcggg cgttgcacat gaatcttaag gtcctcttgc cgtacaaggt cttcgccgaa
     8101 aagcgcggcg tgtcgcgcat cgtcgcacgg accggcgagg gttcgttcgg actcttgccc
     8161 aggcggctcg actgcgtggc ggcactggtg ccggggatac tgactttcga gacgcctgag
     8221 gagggggagg tttacatcgc cgtcgatgag ggcatcctgg tcaaagccgg caaggatgtg
     8281 ctgatctcgg tgcgcaacgc gattggcggc atggatctgg ataaactgca tgcggcggtg
     8341 gggcgggagt tcttggcgct ggatgatcag gaaaaaatgc tgcgttcggt gttggctaag
     8401 ctggagagag gattcattcg tcgtttcgcg gagttccatc atgagtgaag aacggcacaa
     8461 caaagcgtta ccggacgaaa ccaaattcag ccgtcaggta ggcgagatgg cctcgctcaa
     8521 actcaaggcg caacgtcacg ccactcaaac cgtgtgggcc ggtctgggca tgatggggct
     8581 ggtcggctgg tcggtggcga tcccaacgct gctcggtgcc gcactcggca tctggatcga
     8641 cgaacactat cccggcagtc actcctggac gctggcattg ctggccatcg ggctgggact
     8701 ggggtgcttc aatgcctggc actgggtgtc caaggaagaa aaagaaatcc atgcggaggg
     8761 cggcggtcat gactgatcca ttgggtctcg tctcggcttc ggctatgggg tttctgctgg
     8821 gtgcattctt cttcggcggc ctgtggtgga ccgtgcgccg tggggtggca tcggagcggg
     8881 tggcgctctg gttcttcggc agcatgctgc tgcgaaccgg tgtcgtgatg ctcggattct
     8941 atttcctgat gggcgacagt tggcaaagac tgctggcggg cctgtttggc ttcgtgctgg
     9001 cgcgcatggc cgtgacgcgt ctgaccggac aggcgaaagt gtcggcgcgg gaggcggagc
     9061 atgcacctta gtcccgacga gatcatcttc tggcaaagcg ggttattcaa actcaatgcc
     9121 accatcgttt atacatgggg gctgatggct gtgctggtga tcggctcaaa actcgtcacg
     9181 cgcaaacttt ccatggggct gcaacgcacc cgttggcaga atctgttgga gatcgtcgtg
     9241 agcggtatcg agaaacagat cggagaagtc ggtctgcgtc ggccgcaaaa atatatcggc
     9301 tttctgggca cgctgttttt gtttgtcgcc tcggcaagcc tgttcaccat tgttcccggt
     9361 tatgagccgc cgacgggctc gctttcgacc actgccgcgc tggcggtctg cgtgttcgtg
     9421 gcggtgccgt tgttcggtat cggggcgagc ggtctgggcg agtatctccg ggaatatttg
     9481 cagccgacct tcatcatgtt gccgttcaat atcatcagcg agctttcgcg cacgctggca
     9541 ctggctgcgc gcctgttcgg caacatgatg agcgggacga tgatcctcgc tattctgctg
     9601 accattacgc cttatgtatt tccggtcgcg ctgagtatgc tcggcttgct gaccggcatg
     9661 gtgcaagcct atatcttcag tattctggct gcggtctaca tcgcggcggc cacgcgatca
     9721 ggcaggccgg attccgctgt aacagatgaa aaatgaatac tcaccaggaa aggaaatgcc
     9781 atggatagca cgaccattat cgcagtcgca tcgatcgtca cggcgggtct gaccatcgcc
     9841 atcgggagca tcggccccgc gctgggcgaa gggcgcgcgg tctccacggc gctgacctcg
     9901 ctggcgcagc aacctgatgc ctcggcgacg atcacccgca ccctgttcgt gggactggcc
     9961 atggtcgagt ccatcgcgat ctactgtttc gtggtctcga tgatcctact gttcgccaac
    10021 ccgttctgga accatgccat cgctcaggcc gccggaaagt aagccatgct gatcgactgg
    10081 ttcaccgtca tcgcgcaggt cttcaacttc ctcattctgg tgtggttgtt gaagcgcttc
    10141 ttgtaccgtc ccattctcga tgcgatcgat gcaagggaga aacggatcgc cgcagaactg
    10201 gcggatgcgg atgcgaaaaa agccgaggcc ctgaaggagc gcgacgagtt ccagcgcaag
    10261 aacgaagcgt tcgaccagca gcgtgccgcc ctgttgggcc gggcaatgga tgaggcgaag
    10321 gccgagcgtc agcgtctgct cgacgaggca cgcaaggcgg cggatgagtt gagtgccaag
    10381 cgccaggaaa tcttgaggag cgaagcccac aacctgaatc aggcgctcgg cagccgggcc
    10441 aggcaggagg tctttgccag tgcgcgcaag acgctggccg atcttgcctc cgtgagcctg
    10501 gaagaacgca tgggcgaagt gttcatccgc cgcctgcgcg agatggacgg caaggggaaa
    10561 gcaggcctgg gcgaggccct taagtcagcg tcgtctccag ggatcgtgcg cagcgcattt
    10621 gacctgcctg cggagcaacg ggc
//
LOCUS       DASUIV010000169         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_969162, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000169 DASUIV010000000
VERSION     DASUIV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969162"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFW59_06275"
     CDS             complement(<1..547)
                     /locus_tag="VFW59_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5363854.1"
                     /translation="MAILSTGALRQGRAISFLLAGSVMWGLFWWPLKAFDAAGIHVSF
                     VQAIAYAAATLVLLPLVWRSWPEWRDQLGLVLLICLLGGWANASFATSLAEGNVVRVM
                     LLFYLAPVWTILAARIFLAEPFTALRLSALALALTGLVATLGGAEMFATPLSLVDLLA
                     LSSGLAFALNNVAIRVGHNLPE"
     gene            complement(613..1590)
                     /locus_tag="VFW59_06280"
     CDS             complement(613..1590)
                     /locus_tag="VFW59_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5363855.1"
                     /translation="MDIKKLIATVALLTASFSVGAATPVKIALTTWIGYSPFYVAEGM
                     DMYKKHGLKVTLQTFADPAMLPSAVAGKAVDGALMTYDQVIGAAAQGQMFKVVMPIDY
                     SNGGDAVLADMSIKKIPDLKGKKVAYAPLSPSDFLISYALKINGMSDKDISPVNMTPE
                     AIPSAMASKAVPAGATYEPNVSQILAMGGGKQFHVIYSSKQAPGLIADVLVFEDAYIK
                     KNGKEVAALIKAYLDGLAYLKSHPKEASEIIGKAIGVSGKEVLAQLEGVYNIPLKEMA
                     LPFKPGKDTKSYFVSGEVINEILLKSGQIKKPVAIAATLDDQFVKALSK"
     gene            complement(1797..>2321)
                     /locus_tag="VFW59_06285"
     CDS             complement(1797..>2321)
                     /locus_tag="VFW59_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX5363856.1"
                     /translation="FGESQEIAARMGIACSFETHRSRSLFNPWVTLAVLERLPELKLT
                     CDFSHWVVVMERQLDDDWDAVQAVAKHAHHIHARVGYDQGPQVPHPAAPEYAGALASH
                     QRCWEAIWDAQRAAGFSVATMTPEFGPDGYLHTLPFTQQPVADLWQINAWMGKTEQTH
                     FLEWNSRIAQGGDA"
BASE COUNT          451 a          724 c          688 g          458 t
ORIGIN      
        1 tttccggcag gttgtgcccg acccggatcg cgacgttatt caacgcgaag gcgaggccgg
       61 aggagagcgc cagaagatcg acgagcgaca gcggcgtggc gaacatctcc gctccgccca
      121 gcgtcgcaac cagtcccgtc agtgctagcg ccagcgcgct aaggcgcaat gcggtgaagg
      181 gctcggccag gaaaattcgt gccgccagta tcgtccacac cggggccaga tagaacagca
      241 gcatcacccg caccacgttg ccttcggcca gcgaggtggc gaacgatgcg ttcgcccagc
      301 cgcccagcag gcagatgagc agcaccagtc cgagctggtc gcgccattcc ggccaactgc
      361 gccagacgag aggcagcaac accagcgttg ccgcagcgta ggcgatggct tgcacaaagc
      421 tgacgtggat acctgcagca tcgaatgcct tgagcggcca ccagaacagg ccccacatga
      481 ccgagcccgc gagtaaaaac gagatcgctc tgccttgccg caaagcgcca gtcgaaagaa
      541 tagccatcgg atgtcccggt agaaaaagaa acgcccgccc cgaccatggc caaggcgggc
      601 gaatggagcc gcttatttcg acaatgcctt cacgaactgg tcgtcaagcg tggcggcaat
      661 cgccaccggc ttcttgatct ggccgctctt cagcagaatc tcgttgatga cttcgccgct
      721 gacgaagtag gacttggtgt ccttgcccgg cttgaacggc agggccatct ctttcagcgg
      781 gatgttgtac acgccttcca gctgcgccag cacttccttg ccggacacac caatggcctt
      841 gccgatgatc tcggaagctt ccttcgggtg cgacttcagg tatgccagac cgtccagata
      901 ggccttgatc agcgccgcga cttccttgcc gttcttcttg atgtacgcgt cctcgaacac
      961 cagcacgtcg gcgatcaggc cgggagcttg tttggacgaa tagatcacgt ggaactgctt
     1021 gccgccgccc atcgcgagga tctgcgacac gttcggctca taggtcgcac cggcgggcac
     1081 agccttggat gccatcgccg aggggatcgc ttccggcgtc atgttgaccg gactgatgtc
     1141 cttgtcgctc atgccgttga tcttcagcgc ataggagatc aggaagtcgg agggagacaa
     1201 aggtgcataa gcgaccttct tgcccttcag gtcggggatt ttcttgatcg acatatccgc
     1261 cagcactgcg tcgccgccgt tggaatagtc gatgggcatc accaccttga acatctggcc
     1321 ctgcgccgcc gcaccgatca cctggtcgta ggtcatcagc gcgccgtcga ccgccttgcc
     1381 cgcaacggcg gaaggcagca tcgccgggtc ggcgaaggtt tgcagggtca ctttcagacc
     1441 gtgcttcttg tacatgtcca tgccttccgc aacatagaac ggcgagtagc cgatccaggt
     1501 ggtcagcgcg atcttgaccg gcgtagcggc gccgacagag aacgaagccg tcagtaacgc
     1561 gacggttgca atgagtttct tgatatccat ttggcaatct cctgaattaa attaacgaac
     1621 cgattggcta gggtggtgtt cgccctttcc gacttccata cctttcgcag caggttgcat
     1681 gccaaccttg ccgcgcggcg ttctggcctg ttgcgccgtt gctgctactg cgtgatgcag
     1741 atactttaag cgcgctatac gggcataccg cccggcgggt gcacgaaatc gggcgttcag
     1801 gcatctcctc cttgtgcgat gcggctgttc cattcgagaa aatgtgtctg ttccgttttg
     1861 cccatccatg cattgatctg ccacaggtcg gcgaccggct gttgcgtgaa cggcagggta
     1921 tgcagataac cgtccggacc gaactcgggc gtcatcgtgg cgacggaaaa tcctgctgcg
     1981 cgttgcgcat cccagattgc ctcccagcag cgttggtgcg atgccagcgc accggcatat
     2041 tctggcgcgg cggggtgcgg cacttgcggt ccttggtcgt agccgacgcg ggcatggatg
     2101 tgatgcgcat gcttcgccac tgcctgcacc gcatcccagt cgtcatccag ttgtcgctcc
     2161 atcaccacca cccagtggct gaaatcgcag gtgagcttca attccggcaa gcgctccagc
     2221 acggccagcg tgacccacgg gttgaacagc gagcggctgc gatgcgtctc gaagctgcat
     2281 gcgatgccca tgcgcgccgc gatctcctgc gactcgccga a
//
LOCUS       DASUIV010000170         5342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_412928, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000170 DASUIV010000000
VERSION     DASUIV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412928"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..689)
                     /gene="nusB"
                     /locus_tag="VFW59_06290"
     CDS             complement(219..689)
                     /gene="nusB"
                     /locus_tag="VFW59_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5363857.1"
                     /translation="MSEGVNGLEVQKAPSKSPRHRARELALQGIYQWRVSGSDVQDIE
                     KHTLGEKNLGRYDKPMFSRLLRGALAQHESVEALLTPHLDRPMSELSPVEFSVLLLGA
                     FELSQHLEVPYKVVINEAVELAKTFGGTDGHKYVNGVLDKLAPLVRAVEFAARK"
     gene            complement(686..1138)
                     /gene="ribH"
                     /locus_tag="VFW59_06295"
     CDS             complement(686..1138)
                     /gene="ribH"
                     /locus_tag="VFW59_06295"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5363858.1"
                     /translation="MKEIKKNLDGKGLRIGIVQSRFNTEVCDGLLGACRAQLVQHGVA
                     EDDITLATVPGALEIPLVLLTMAESGDYDALIALGAVIRGDTYHFEVVSNESARCVGD
                     VQLVSRTPIANAILTTDTDEQAVARMHIKGGEAALVAIEMVNLLRGLS"
     gene            complement(1138..2235)
                     /gene="ribBA"
                     /locus_tag="VFW59_06300"
     CDS             complement(1138..2235)
                     /gene="ribBA"
                     /locus_tag="VFW59_06300"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX5363859.1"
                     /translation="MSDVAPIQEIIEEIRAGRMVVLVDEEDRENEGDLVFAAEFVTPE
                     MVNFMAKHGRGLICLTLTQSHCDQLNLPLMVSANGLPLATNFTVSIEAASGVTTGISA
                     ADRARTIQVAANKHAKPADIVQPGHIFPLRAQNGGVLVRAGHTEAGCDLAQLAGLTPA
                     SVICEILKDDGEMARLPDLIPFAKQHGLKIGTIADLIEYRVHNETLIERVARRTVQTA
                     HGELDLVTYVDQTTQRTHLALVKGDIRPEHETLVRVHEPLSVMDFLEQTSYPNSTSVH
                     EALARIAAAPAGVLVLMHHPETSQALLARAAAKPEAHHAQWDPRNYGIGAQILRDLNV
                     GKMCLIATPRKLPSMTGFGLEIVRYCHNKEQ"
     gene            complement(2232..2837)
                     /locus_tag="VFW59_06305"
     CDS             complement(2232..2837)
                     /locus_tag="VFW59_06305"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5363860.1"
                     /translation="MFSGIIADVGVIKSAQDRDGGLRLTVATEALGMGDVQLGDSIAV
                     NGVCLTVVKIDGNMFTVDVSRETLNCTVGLGAQGARVNLEKALRLSDRLGGHLVSGHV
                     DGVGDVVSFTDIGESWRLAVRAPQELAKFIAVKGSVTINGVSLTVNEVVGAVFNINLI
                     PHTLAMTTLNELKAGAQVNLEIDLIARYVERMMNAEKEQTK"
     gene            complement(2988..3317)
                     /locus_tag="VFW59_06310"
     CDS             complement(2988..3317)
                     /locus_tag="VFW59_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5363861.1"
                     /translation="MTSALGFAGGKKVMLKQPCVYILASQRNGTLYVGVTSDLVKRIW
                     EHRNDTVDGFTKKYSVHTLVYYELHEDMLTAITREKQIKKWNRDWKIKLIESVNPEWR
                     DLWEDIV"
     gene            complement(3442..4530)
                     /gene="ribD"
                     /locus_tag="VFW59_06315"
     CDS             complement(3442..4530)
                     /gene="ribD"
                     /locus_tag="VFW59_06315"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5363862.1"
                     /translation="MFDAADSRWMAQALRLAERGLCTASPNPRVGCVLVKHAALVGEG
                     WHVRAGEPHAEVHALRAAQEAARGATAYVTLEPCSHHGRTPPCADALIAAGVTRVVIA
                     MQDPNPQVAGQGIAKLRAAGIPVECGLMEAAARELNIGFCARMTRGMPWVRSKIGMSL
                     DGRTALANGVSQWITGAAARQDVQHWRARSCAVLTGIGTVLADDPQLNVRDRVVERQP
                     MRVVLDSELQMPPSACILQGGGVVIYTAVQDAQKSAMLEKAGATVCMLPGAEGRVDMD
                     AALRDLAQRGVNEVLVEAGSTLNGALLKAGLVDELLLYVAPQLLGDAARGMAALGELT
                     RLDQRVDLKWQDVRQIGDDLRITVRVER"
     gene            complement(4530..5009)
                     /gene="nrdR"
                     /locus_tag="VFW59_06320"
     CDS             complement(4530..5009)
                     /gene="nrdR"
                     /locus_tag="VFW59_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX5363863.1"
                     /translation="MKCPFCKGDNTQVVDTRESEEGDSIRRRRRCPSCDKRFTTYETV
                     ELRMPQVVKQNGIRSEFSVAKLRTSFSRALHKRPVSTELVDAAIDHVTQKIFSLGERE
                     IGSRLIGEMVMKELLKLDKVAYIRFASVYKSFQDVGDFTDAVDEVSEPPTRKAPKKS"
     gene            complement(5102..>5342)
                     /gene="glyA"
                     /locus_tag="VFW59_06325"
     CDS             complement(5102..>5342)
                     /gene="glyA"
                     /locus_tag="VFW59_06325"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749062.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5363864.1"
                     /translation="AHITVNKNAIPNDPQKPFVTSGIRIGSPAMTTRGFKELEAEKLA
                     HLIADVLDAPNDDAVIARVVGEVKKLTAQFPVYGA"
BASE COUNT         1016 a         1786 c         1450 g         1090 t
ORIGIN      
        1 gaccgccagc accttgcccg ccgaaggttg cagcagcgcg gcatcgtcgc cgacacccag
       61 cagggtatgg ggcgtggcac ggctgaaatg gcggcggatc agatcgaatt cagacatgac
      121 tggggagtgg aaagtggaaa gtggggagtg gggggcagct ttccccactt accactgagg
      181 cgcgaagcgc cgttccactc accactcact gctctccatc atttgcgtgc cgcgaattcc
      241 acggcgcgca ccaatggcgc cagcttgtcg agcacaccgt tcacatattt gtggccgtcg
      301 gtaccgccga aggtcttggc caactcgacc gcctcgttga tgaccacctt gtaaggcact
      361 tcgagatgct gcgacaactc gaatgcaccg agcagcaaca cggaaaattc cactgggctg
      421 agttcggaca tcggacgatc cagatgcgga gtcaacagcg cctcgacgct ctcgtgctgc
      481 gccagcgcgc cgcgcaacaa ccgggagaac atcggcttat cgtaccgacc gagattcttt
      541 tcaccgagag tatgcttctc gatgtcctgc acatcgctgc cggacacgcg ccactggtag
      601 atgccttgca gcgccagttc gcgggcacga tgacgcgggc tcttgctcgg cgccttctgg
      661 acttcgagtc cgttcacgcc ctcgctcatg acaagcctcg cagcaggttg accatctcga
      721 tggccaccag cgcggcctcg ccgcccttga tgtgcatgcg cgcgacagcc tgttcgtcgg
      781 tatcggtggt gaggatggca ttggcgatcg gcgtgcggct aaccagctgc acatcgccca
      841 cgcaacgcgc cgattcgttc gataccacct cgaagtgata ggtgtcgcca cggatcaccg
      901 cgcccagcgc gatcagcgca tcgtaatcac cgctctccgc catggtcagc agcaccaagg
      961 ggatttcaag cgcgcccggc acggtcgcca gcgtgatgtc gtcttccgcc acgccgtgct
     1021 gcaccagttg cgcgcggcat gcgcccagca aaccgtcgca cacctcggtg ttaaagcggc
     1081 tttgcacgat accgatacgc aggcccttgc cgtccagatt ctttttgatc tctttcatta
     1141 ctgctccttg ttatgacaat agcgcacgat ttccagaccg aaaccggtca tgctgggaag
     1201 tttgcgcggg gtcgcgatca gacacatctt gccgacattc aggtcgcgca gtatctgcgc
     1261 gccgataccg taattgcgcg ggtcccattg cgcgtgatgc gcttccggtt tggcggcggc
     1321 gcgcgccaac agcgcctgcg aagtctcggg gtgatgcatc agcaccagca ccccggcagg
     1381 tgccgccgcg atacgggcca gcgcctcatg cacgctggtg gaattcgggt agctcgtctg
     1441 ctcgagaaaa tccatcaccg acagcggctc gtgtacgcgc accagcgtct catgctcagg
     1501 ccggatatcg cccttgacca gcgccagatg ggtgcgctgc gtggtctggt ctacataggt
     1561 caccagatcg agctcgccgt gagcggtctg caccgtgcgg cgcgccacgc gttcgatcag
     1621 cgtctcgttg tggacgcggt attcgatcag gtcggcgatg gtgccgatct tcagcccatg
     1681 ctgtttggcg aaagggataa ggtcgggcag acgcgccatc tcgccgtcgt ccttgagtat
     1741 ctcgcagatc accgaagcgg gcgtcagtcc ggccagttgc gccagatcgc agccagcctc
     1801 ggtgtgtccg gcacgcacca gcacgccgcc gttttgcgcg cgcaacggga agatgtggcc
     1861 gggctgcacg atatcggcag gcttggcgtg cttgttcgcc gccacctgta tggtgcgcgc
     1921 gcgatccgcc gccgagatac cggtggtcac gccgctcgcc gcctcgatgg acaccgtaaa
     1981 attagtcgcc agcggcaggc cgttggcgct caccatcagc ggcaggttga gctgatcgca
     2041 atgcgattgc gtcaaggtca gacagatcag cccgcgtccg tgtttggcca tgaagttgac
     2101 catctccggc gtgacgaatt cggcggcgaa caccagatcg ccctcgttct cgcgatcctc
     2161 ctcgtccacc agcacgacca tgcgcccggc gcgtatttct tcgatgattt cctgtatcgg
     2221 tgcgacgtcg ctcatttcgt ttgttctttc tctgcattca tcatgcgttc cacatagcgc
     2281 gcaatcagat cgatctccag attcacctgc gcgcccgcct tcaattcgtt caacgtcgtc
     2341 atcgccagcg tgtgcgggat caggttgata ttgaataccg caccaactac ctcattaacg
     2401 gtgagactga ctccattgat tgtgaccgag cctttcaccg cgatgaattt cgccagttcc
     2461 tgcggcgcac gcaccgcaag ccgccagctc tcgccgatgt cggtgaacga caccacgtcg
     2521 cccacgccat ccacatgacc gctgaccaga tgcccgccca gccgatccga caagcgcaac
     2581 gccttttcca gattgacccg cgcaccctgc gctcccagcc cgacggtgca attcaaggtc
     2641 tcgcgcgaca cgtccacggt gaacatgttg ccgtctatct tcacgacggt cagacacacg
     2701 ccgttcaccg cgatgctgtc gccgagctgc acgtcaccca tgccgagcgc ctcggtcgca
     2761 acggtcagac gcaagccgcc atcgcgatcc tgcgcggatt tgatgacgcc gacatcggcg
     2821 atgattccac taaacatacc tttactcctt tactccgtca ttccggcgca ggccggaatc
     2881 cagcgatcaa gatattcccg cgtagcggac aaaatctgta gttgttccgc ttcgcggcat
     2941 ttcgcattct gctggattcc cgcgttcgcg ggaatgacaa caatgaatta cacgatgtct
     3001 tcccacaaat ctcgccattc cggatttacg gattcgatca atttaatctt ccagtcccga
     3061 ttccacttct tgatctgttt ttcgcgcgta atggcggtca acatatcctc atgcagctcg
     3121 taataaacca aagtgtggac actatatttc ttggtaaaac catcaaccgt gtcgttcctg
     3181 tgttcccaga tacgcttcac cagatcactg gttaccccga cgtaaagcgt accgttgcgc
     3241 tggcttgcga gaatgtaaac acaaggctgc ttgagcataa ccttcttacc tcccgcaaaa
     3301 ccaagagcgc tcgtcattcc cgcgcaggcg ggaatccagc tttaaaaatt tgcgcgcgag
     3361 cgcaacaaaa ccgctcaaga attggccgct acgcggaatt gttatattgc tggattcccg
     3421 cctgcgcggg aatgacaccc tttatcgttc cacccttacc gtgatgcgca gatcgtcccc
     3481 gatctggcgc acatcctgcc atttgagatc gacgcgctga tccagtcgcg tcagctcacc
     3541 caatgccgcc atgccgcgtg ccgcatcgcc gagcagctgc ggcgcgacat acagcaacaa
     3601 ttcatccacc aagcccgctt tcagcaaggc gccgttcagc gtgctaccgg cctccaccag
     3661 cacctcgttg acaccccgtt gcgccaaatc acgcaacgca gcatccatat ccacccgccc
     3721 ttccgcaccc ggcaacatgc agaccgtcgc ccctgccttt tcaagcatgg cgctcttctg
     3781 cgcatcctgc acggcggtat agatcaccac cccgccgccc tgcaagatgc aagccgacgg
     3841 aggcatttgc aactcgctgt ccagcaccac gcgcataggc tgacgctcga ccaccctgtc
     3901 gcgcacattc agttgcgggt cgtccgccag caccgtaccg ataccggtca acaccgcgca
     3961 ggagcgtgcc cgccagtgct gcacatcctg ccgcgccgcc gcgccggtga tccattggct
     4021 caccccgttg gccagcgcgg tgcgtccgtc cagactcatg ccgatcttgc ttctgaccca
     4081 gggcatgccg cgtgtcatgc gcgcgcagaa accgatattc agttcacgtg ccgccgcttc
     4141 catcagtccg cactcaaccg ggattccggc ggcgcgcagc ttcgcgatac cctgccccgc
     4201 cacttgcgga ttcgggtcct gcatcgcgat caccacgcgc gtcacaccgg cagcgatcag
     4261 cgcatcggca cagggcggcg tgcgtccgtg atgactgcag ggctccagcg tcacataggc
     4321 cgttgcgccg cgcgccgcct cttgcgcggc acgcaaagca tgcacttcgg catggggctc
     4381 gcccgcacgc acatgccagc cttcaccgac caatgcagca tgctttacca gcacgcagcc
     4441 gacgcgcgga ttagggctgg cagtacacag accgcgctcg gccaatcgta acgcctgcgc
     4501 catccagcgg ctgtctgcgg catcgaacat caggatttct ttggcgcctt gcgtgtcggc
     4561 ggctcactca cctcgtccac cgcatcggtg aaatcgccca catcctgaaa gctcttgtac
     4621 accgaggcaa aacggatgta ggccacttta tcgagcttga gcaattcctt catcaccatc
     4681 tcgccgatca ggcgggaacc gatctcgcgc tcccccagcg aaaatatctt ctgcgtgaca
     4741 tgatcgattg cggcatccac caactcggtc gataccggac gcttgtgcag cgcacgcgaa
     4801 aagctggtgc gcaacttggc cacactgaat tcgctacgga taccgttctg cttgaccacc
     4861 tgaggcatgc gcaactccac cgtctcatag gtagtaaaac gcttgtcgca cgacgggcag
     4921 cggcggcggc gacgtatgct gtcgccttcc tcgctttccc gcgtgtccac cacctgggtg
     4981 ttatcgcctt tgcagaacgg acatttcatg gctgagtggt cagtagtgag tagtcagtag
     5041 tgagtggcga gggcggcaga aactcaccca ctcaccactt cccaatttcc actcacccgc
     5101 tttaggctcc gtagaccggg aactgcgccg tcagcttctt cacttcgccc acgacgcgag
     5161 cgatcaccgc gtcgtcgttc ggcgcgtcca gcacatcggc gatcaggtgt gcgagcttct
     5221 ccgcttccag ttccttgaag ccgcgcgtgg tcatcgccgg actaccgata cggatgccgc
     5281 tcgttacgaa cggtttctgc gggtcgttcg gaatggcgtt cttgttgacg gtgatatggg
     5341 cg
//
LOCUS       DASUIV010000171         8965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1156813, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000171 DASUIV010000000
VERSION     DASUIV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156813"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFW59_06330"
     CDS             <1..265
                     /locus_tag="VFW59_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363865.1"
                     /translation="DVPSMALTRQNGGNSIAVYKSGRGLREKKTLDTCKKLLQAERVN
                     FIAPADYIKDSELERRVQLLLKSIIAKIEYEKELFDCRKKHAI"
     gene            284..745
                     /locus_tag="VFW59_06335"
     CDS             284..745
                     /locus_tag="VFW59_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEX5363866.1"
                     /translation="MILSSLSQSSRYAALHPLFTRAFDYIRDTDLMALKPGRHDIVGD
                     DLFAIVEQVPGRTREAAQLEAHRRYIDIQLVLEGVDEMGWKPLADCHDPVSEHSAERD
                     IRFFRDEPAAWIATPPGHFCIFFPEDAHAPLVSSSTIRKVIFKVTVNPAGL"
     gene            complement(784..1635)
                     /gene="ttcA"
                     /locus_tag="VFW59_06340"
     CDS             complement(784..1635)
                     /gene="ttcA"
                     /locus_tag="VFW59_06340"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX5363867.1"
                     /translation="MNDIVQPIHFESRSTNFNRLKGRLESSVGKAIGDFNMIEDGDTV
                     MVCLSGGKDSYTLLDILLTLRKRAPIDFRIVAMNLDQKQPGFPADVLPNYLKNLGVEY
                     HIETQDTYSIVKEKIPEGKTTCSLCSRLRRGIIYKVAGELGANKIALGHHRDDMVETL
                     FLNMFFGGKLKAMPPKLVTDKGDHIVIRPLAYCAEKDIARYARGMEFPIIPCNLCGSQ
                     ENLQRQNIKEMLTNWERQYPGRSQTIFTAMQNVKPSHLLDGSLFDFKNMQLGAVVEEG
                     DVAFDDH"
     gene            complement(1643..2383)
                     /locus_tag="VFW59_06345"
     CDS             complement(1643..2383)
                     /locus_tag="VFW59_06345"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEX5363868.1"
                     /translation="MHNKVVLVTGGAKRVGAAICRRLHASGAMIAIHYRHSAAEAQAL
                     QDELNALRADSAALFQADLIDTQALPQLVAAVVGQFGQLDALVNNASSFYATPLAEID
                     ETQWGDLLGTNLKAPLFLAQAAAPELRRRHGAIVNIVDIHAERPMQGHLLYSVAKAGL
                     VALTKGLAQEMSPQVRVNAVAPGVIVWPEGGEWEDVERRRKIVAHTLLKREGEPDDIA
                     RTVRFLLNDAPYITGQVIAVDGGRSVNI"
     gene            2449..3603
                     /locus_tag="VFW59_06350"
     CDS             2449..3603
                     /locus_tag="VFW59_06350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5363869.1"
                     /translation="MSDHLPSPSPEAAQHSAQLTDLIRRDIAAHGGWISFARYMELAL
                     YAPGLGYYTAGAHKFGEAGDFTTAPELSALFGRTLARQLAEIMAHSTPHILELGAGSG
                     KLAADLLAELEQLGSLPDSYSILEISADLRERQQALLRERLPHLSGRVRWLDALPESF
                     GGAMIANEVLDALPVHLVHWQDSALSERGVALSGEGFCWQDSPINDGDLLQAAQKIAV
                     PDGYVSEICLAQRGLINSLAQRLEQGALLFIDYGFGAREFYHPQRSSGTLMCHYRHRA
                     HGDPFFLPGLQDITAHVNFTDVAECGIDAGLELLGYTSQAFFLINCGITELLSGTSPE
                     QVRDYLPLSAQLQKLTSPAEMGELFKVIALGKEMANPLCGFARGDLSRLL"
     gene            complement(3605..4081)
                     /locus_tag="VFW59_06355"
     CDS             complement(3605..4081)
                     /locus_tag="VFW59_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX5363870.1"
                     /translation="MMKRMAYLYLTLAMASPLAVAADGVDALLENKEPATPIERAFNK
                     LDANHDGVISRGEVQLKQTALLLDNFAKIDENKDDGISLQEFQKFLETLRAKQEEFFN
                     RLKAADKNGDGEWTLKELKSAKAKLPSLEKNFDAIDSNHDKKITAQEIDAFAHAIR"
     gene            complement(4078..5322)
                     /locus_tag="VFW59_06360"
     CDS             complement(4078..5322)
                     /locus_tag="VFW59_06360"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEX5363871.1"
                     /translation="MTDKSAIYCVGGAVRDRLLGLPVTDRDWVVVGSTPEEMEAQGFR
                     PVGRDFPVFLHPQTHEEYALARTERKTAPGYKGFAIHASPEVTLEEDLLRRDFTVNAI
                     AQDADGNLIDPHGGIADLHAGVLRHVSDAFAEDPVRILRGARFAARFDFAIAPETLAL
                     MRKMVADGEVDALVQERVWQELARGLMEAKPSRFFATLRACGALARILPEVDALFGVP
                     QPEKYHPEIDSGIHTLMVVDDAASQHFALEVRFAALTHDLGKATTPPELLPRHIGHEL
                     RGVELLKILCERLRVPADCRDLALLAARYHGDIHRAFELRSETVIRLFQSADAWRKPE
                     RFAQLLQACSADARGRTGHEQDAYPQADYLLQLMQIARAVDAGKIASDCADKATIPDR
                     VRQARIAAIEQTVNECRQTKGQ"
     gene            complement(5331..7295)
                     /locus_tag="VFW59_06365"
     CDS             complement(5331..7295)
                     /locus_tag="VFW59_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5363872.1"
                     /translation="MRFILYFLLAFVPAALAAQPSKHLPPEMAATDDAAFLAAHDAFR
                     AGDRAKLDRLAPRFKKTPLEVYISYYQLRLGLEQADPQAVRDFLARPEDTPMVDRLRG
                     EWLKQLANRQQWQLFDAEYPRLLDEDAELACYALQSRRRSDEQAALQEARRLWFSGKG
                     QPDSCGPLFDAAIGAGIIVEQDIWQRLRLALEAGNVSLAKQLADKLTDAAGLATALGR
                     AAADAEGYLDKLSLDKDSRGQRTVVLFALQRLAKQSPDLAFTRWQRFAANFSEAEQHY
                     FFGWLAYEAARKQDGRALQWYRAAMGEPLSEQQAAWRVRAALRAYDWSAALMAINAMS
                     PQQQRDPAWQYWKARALQVMGKPQDAQKLLVSLSAGRHFYGQLASDELVGVPVLSAAE
                     PAYKSTPQDIASTQVLPGIQRTLALYRMGMRTEASKEWSWTLRNFNDRELLTAAEIAR
                     RNEMYDRAINAADRTVSTHDFSLRYLAPYRDALQGHIREQGLDEAWVYGLMRQESRFV
                     TAAKSNVGAAGLMQIMPSTASWLARKLGLKDYRGELIHQLDTNLKLGTYYMKTVLSWF
                     DDSPVLASAAYNAGPSRARRWRGDFPLEGAIYAETIPFDETRDYVKKVMSNTVYYAAQ
                     FGNAPRSLKQRMGVVAAKAPDNQQAIPDEK"
     gene            7397..>8965
                     /gene="msbA"
                     /locus_tag="VFW59_06370"
     CDS             7397..>8965
                     /gene="msbA"
                     /locus_tag="VFW59_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEX5363873.1"
                     /translation="MKQMTSTQLYLRLLGYVKPYWRVFAAAILGMAVSAATEPLLPAL
                     LKPLLDGTFVNKDDAVIRWAPVLLLIIFFVRGIASFVGTYAIHWVGNRLVMDLRNKMF
                     NKLLALPTRFYDDHATGNLISKLTFDVTNVTAAATSVVTITIRDSIIVVGLLGWLFYL
                     NWQLTLLSLLVAPVVALVISTINRRLRATSRDSQRAMGDVTQVIEESVTAHKVVKLFG
                     GQRYESERFHEQANWVRRHAVKQAAAASANVPIVQMVAAIALSIIIYIATEQSRSNEI
                     TVGAFLSFVTAMLMLTSPLKRLTGVSEFMQRGLAAAESVFELLDTDGEVDNGKIEIGR
                     ASGHLSFEKVAFSYQIDDRLALRDVTLDIPAGQTVALVGASGSGKTTLANLVPRFYTP
                     TSGRITLDGQDITELTLASLRANIALVSQEVVLFNDTLSANIAYGQMREVPQTEIVAA
                     ATAAHALEFINDMPQGFDTLIGEKGVRLSGGQRQRIAIARAILKDPPILILDEATSAL
                     DTESESLVQQALFNL"
BASE COUNT         1640 a         2936 c         2687 g         1702 t
ORIGIN      
        1 cgatgttccc agcatggcac ttacacgcca gaacggtggc aactctatag cggtttacaa
       61 atcagggcgg ggtttgcgtg agaagaaaac tttagacacc tgcaaaaaac tcttacaggc
      121 tgaaagagta aactttattg cgccagctga ttatattaag gactcggaac tggaacgccg
      181 ggttcagttg ctacttaagt ccatcatcgc caaaattgaa tatgaaaaag agttgtttga
      241 ttgtcggaaa aaacatgcca tataaatcta cgcagttaac tatatgatcc tctcctccct
      301 ctcccagtct tcccgctacg ccgcgctgca tccgctgttc acgcgcgcct tcgactacat
      361 ccgcgacacc gatctgatgg cgcttaaacc ggggcggcac gacatcgtcg gcgacgacct
      421 gttcgccatc gtggagcagg tgccgggacg cacgcgcgag gcggcgcaac tggaggccca
      481 tcgccgttac atcgacatac agctggtgct ggagggagtg gacgagatgg gctggaagcc
      541 gctggcggac tgccacgatc cggtgagcga gcacagtgcg gagcgcgaca tccgcttctt
      601 ccgcgacgaa ccggccgcgt ggatcgcgac cccgcccgga catttctgca tcttcttccc
      661 ggaagatgcc catgcaccgc tggtgagcag cagcacgata cgcaaggtga tcttcaaggt
      721 gacggtgaat cctgcggggc tctaaggcct cgtcattccg gccttcgccg gaatgacgga
      781 agtttagtgg tcgtcgaagg ccacgtcccc ttcttctacc accgcaccca attgcatgtt
      841 cttgaagtcg aacaggctgc catccagcag gtgcgacggt ttaacgttct gcatcgccgt
      901 gaagatggtc tgcgaacgtc ccggatactg gcgctcccag ttggtcagca tctccttgat
      961 gttctggcgt tgcaggtttt cctgcgagcc gcacaggttg cacgggatga tggggaactc
     1021 catgccgcgc gcgtagcggg cgatgtcctt ctcggcgcaa taggccagcg gacggatgac
     1081 gatgtgatcg cccttgtcgg tgaccagctt gggcggcatg gctttcagct tgccgccgaa
     1141 gaacatgttc aggaacagcg tctccaccat gtcgtcgcgg tgatgtccca gcgcgatctt
     1201 gttcgccccc agttcgcccg ccaccttgta gatgatgccg cgacgcaggc gcgaacacag
     1261 tgagcaggtg gtcttgccct ccgggatctt ctccttgacg atggaatagg tatcctgcgt
     1321 ctcgatgtga tactcgacgc ccagattctt cagatagttc ggcagcacat cggcggggaa
     1381 ccccggctgc ttctggtcga gattcatcgc gacgatacgg aaatcgatag gcgcgcgctt
     1441 gcgcagcgta agcaggatgt cgagcagggt ataggagtcc ttgccgcctg acaggcacac
     1501 catcacggtg tcgccgtcct cgatcatatt gaagtcaccg atggcctttc cgaccgagct
     1561 ttccaaacgg cccttgagtc gattgaagtt ggtggagcgg gattcgaaat ggatgggttg
     1621 tacgatgtcg ttcatagtgt tttcagatgt tgacggaacg tccgccatcg actgcgatga
     1681 cttgtccggt gatgtaaggt gcatcgttaa gcaggaatcg cacggtgcgc gcgatgtcgt
     1741 ccggttcgcc ttcgcgcttg agcagcgtgt gcgccacgat cttgcggcga cgctcgacat
     1801 cttcccattc gccgccttcc ggccagacga tgacgccggg agcgaccgca ttgacgcgca
     1861 cctgcggtga catctcctgt gccagcccct tggtcagcgc gaccagtccg gccttggcga
     1921 cgctgtacag cagatgcccc tgcatcgggc gctcggcatg gatgtccaca atattgacga
     1981 tggcgccgtg acggcggcgc aactccggtg ccgccgcctg ggcgaggaac aacggcgcct
     2041 tcagattggt gccgagcagg tcgccccatt gcgtctcgtc gatctccgcc agcggcgtgg
     2101 catagaagct ggaggcgttg ttgaccagcg catccagctg tccgaattga ccgaccaccg
     2161 cagcgaccag ttgcggcagg gcttgcgtgt cgatcagatc ggcctggaac aaggctgcac
     2221 tatccgcacg caaggcattc agctcgtcct gcaacgcctg cgcctcggca gcagagtgac
     2281 gataatggat ggcgatcatc gcgccggacg catgcagacg gcggcatatc gccgcaccga
     2341 cgcgcttcgc gcctcccgtc accagtacca ccttgttatg catgcgtcct tcctcgtttg
     2401 ttcgcgacca agctgctaaa atccagcatc cgcgaattat acccgaccat gtccgatcat
     2461 ctgccctcac ccagtcccga agccgcccaa catagtgcgc aactgaccga tctcatccgg
     2521 cgcgacatcg ccgcccacgg cggctggata tcgttcgcac gctacatgga gctggcgctg
     2581 tatgcgcccg gcctgggcta ttacacggcg ggagcgcaca agttcggcga ggccggcgac
     2641 ttcaccaccg caccggaact gtccgcgctg ttcggccgca cgctggcgcg ccagctggcc
     2701 gagatcatgg cgcacagcac gccgcatatc ctggaactgg gcgcgggcag cggcaaactg
     2761 gctgccgact tgctggccga gctggagcaa ctcggcagcc tgccggacag ctattcgata
     2821 ctcgagatca gcgccgacct gcgcgaacgg cagcaggccc tgttgcgcga acggctgccg
     2881 catctgtccg ggcgcgtgcg ctggctggat gcgttgccgg agagcttcgg cggcgcgatg
     2941 atcgccaacg aggtgctcga tgcgttgccg gtacatctgg tgcactggca ggacagcgcg
     3001 ctgtccgaac gcggcgtcgc gctcagcggc gagggcttct gctggcagga cagcccaata
     3061 aacgatggcg acctgctgca ggccgcacaa aaaattgcgg tgccggacgg ctatgtcagc
     3121 gagatctgcc tcgcccagcg aggtctgatc aacagtctcg cccaacggct ggagcagggt
     3181 gcgctgctgt tcatcgacta cggtttcggt gcgcgcgagt tctaccaccc gcagcgcagc
     3241 agcggcacgc tgatgtgcca ctaccgccat cgcgcccacg gcgatccgtt cttcctgccc
     3301 ggcttgcagg acatcaccgc acatgtgaac ttcaccgatg tcgccgaatg cggcatcgac
     3361 gcgggactgg agttgctggg ctatacctcg caggccttct tcctgatcaa ctgcggcatc
     3421 accgaactgc tgagcggcac ctcgccggag caggtacgcg actacctgcc gctgtccgcg
     3481 caattgcaga aactcaccag cccggcggag atgggcgagc tgttcaaggt gatcgccttg
     3541 ggcaaagaaa tggcgaaccc gttgtgcgga ttcgccagag gcgacctgag ccgattgctg
     3601 taacttaacg gatcgcgtga gcgaacgcgt cgatttcctg cgcggtgatc ttcttgtcgt
     3661 ggttgctgtc gatcgcatcg aagttctttt ccagcgaggg cagctttgcc ttggccgact
     3721 tcagctcctt cagcgtccat tcgccgtcgc cgttcttgtc ggcggccttc agccgattga
     3781 aaaactcttc ctgcttcgcg cgcagcgtct ccagaaactt ctggaattcc tgaagactga
     3841 tcccgtcgtc cttgttctcg tcgatcttgg caaagttatc gagcagcaat gccgtttgct
     3901 tcagctgcac ttcaccgcgc gaaataacgc cgtcgtgatt ggcgtccagt ttattgaagg
     3961 cgcgctcgat cggtgtagcg ggctccttgt tttccagcag cgcatccact ccgtccgccg
     4021 caacggccaa cgggctcgcc atcgcaaggg tcaggtacag gtaagccatt cgtttcatca
     4081 ttgtcctttc gtttgtctgc actcgtttac ggtttgttcg atcgcggcga tacgagcctg
     4141 ccgcacgcgg tcggggattg tagccttatc cgcacaatcg ctggcgatct tcccggcatc
     4201 taccgcgcgt gcgatctgca tcagctgcag caggtaatcg gcctgcgggt aggcatcctg
     4261 ctcgtgtccg gtgcgtccgc gcgcgtccgc gctgcaggcc tgcagcaatt gcgcgaaacg
     4321 ctccggcttg cgccaggcat cggcggactg gaacaggcgg atgacggtct ctgatcgcag
     4381 ctcgaaggcg cgatggatgt cgccgtggta gcgcgccgcc agcagcgcga ggtcgcggca
     4441 gtctgccggc acgcgcaggc gctcgcacaa aatcttcagc aattccacgc cgcgcagttc
     4501 gtggccgata tggcgcggca aaagttcggg cggagtcgta gccttgccca gatcgtgggt
     4561 cagcgcggcg aaacggacct ccagcgcaaa atgctggctg gccgcgtcgt ccaccaccat
     4621 cagggtgtgg atgccactgt cgatctcggg atgatatttc tccggctgcg ggacaccgaa
     4681 cagcgcatcc acctcgggca gtatccgcgc cagcgcgccg catgcgcgca aggtggcgaa
     4741 gaaacgcgac ggctttgcct ccatcagtcc gcgcgccagc tcctgccaga ctcgttcctg
     4801 caccagcgca tcgacttcgc cgtcggcgac catcttgcgc atcagcgcga gggtttccgg
     4861 cgcgatggca aaatcgaagc gtgctgcaaa gcgcgcgccg cgcaggatgc gcaccgggtc
     4921 ttcggcaaag gcatcgctga cgtgacgcag cacacctgca tgcaggtcgg cgattccgcc
     4981 gtggggatcg atcagattgc cgtccgcatc ctgtgcgatc gcattgacgg tgaagtcgcg
     5041 gcgcaacaag tcttcttcca gcgtcacctc tggcgaggca tggatcgcga aacccttgta
     5101 tcccggcgcg gtcttgcgct cggtgcgcgc cagcgcgtat tcctcatggg tctgcggatg
     5161 caggaacacc ggaaagtcgc ggccgaccgg acggaagccc tgtgcctcca tttcctccgg
     5221 cgtgctgccg accaccaccc agtcgcgatc cgtgaccggc agccccagca agcgatcccg
     5281 caccgcaccg cccacgcaat agatggcgga tttatcggtc atgtcggcac tcacttctca
     5341 tccggaatgg cttgctgatt atcgggcgcc ttggcggcga ccacgcccat gcgctgcttg
     5401 agcgaacgcg gtgcgttgcc gaattgcgcg gcgtaataca cggtattgct catcaccttt
     5461 ttcacgtaat cgcgcgtctc gtcgaacgga atggtctcgg cgtagatggc cccctccaga
     5521 ggaaaatcgc cgcgccatct gcgcgcccgg ctaggacccg cattgtaggc cgccgaagcc
     5581 agcaccggac tgtcgtcgaa ccagctcagc acggtcttca tatagtaggt gccgagcttc
     5641 agattggtgt cgagctggtg gatcagctca ccgcgatagt ctttcagacc gagcttgcgg
     5701 gccagccagc tggcggtcga gggcatgatc tgcatcagcc ccgccgcgcc gacattcgac
     5761 ttggccgcag tgacgaagcg gctttcctgg cgcatcagac cgtagaccca cgcctcgtcc
     5821 agcccctgct cgcgaatatg gccctgcaac gcatcgcggt acggggccag atagcgcaga
     5881 ctgaaatcat gggtgctgac ggtgcggtct gccgcattga tggcgcggtc gtacatctcg
     5941 ttgcgccgcg cgatctcggc cgcagtgagc aattcgcggt cgttgaaatt gcgcaacgtc
     6001 cagctccact ccttcgatgc ctcggtgcgc atccccatgc gatacagcgc gagggtgcgc
     6061 tggatacccg gcaacacctg cgtcgaagcg atgtcctgcg gcgtcgattt ataggccggc
     6121 tcggccgcgc tcagcaccgg aacccccacc agctcgtcgg acgccagctg accgtagaaa
     6181 tgccgtccgg cgctcagcga caccagcaac ttctgtgcat cctgcggctt gcccatcacc
     6241 tgcagcgcac gcgccttcca gtattgccag gcgggatcgc gctgctgctg cggactcatc
     6301 gcgttgatcg ccatcagcgc ggccgaccag tcgtacgcgc gcaacgccgc gcgcacccgc
     6361 caggcggcct gctgctcgct gagcggctcg cccatcgcgg cgcgatacca ttgcagcgcc
     6421 cgcccgtcct gcttgcgcgc ggcctcatag gccagccagc cgaagaaata gtgctgctcg
     6481 gcctcggaaa aattcgcagc aaacctttgc cagcgggtaa acgccagatc gggcgactgc
     6541 ttcgccagac gctgcaaggc aaacagcacg accgtgcgct ggccgcgact atccttatcc
     6601 agcgacagtt tatccagata cccctccgca tccgctgcgg cgcgccccag agccgttgcc
     6661 agcccggcag cgtcggtcag cttgtcggcc agctgtttgg cgagcgacac attgcccgcc
     6721 tccagcgcca gacgcaggcg ctgccagata tcctgctcga cgatgatgcc cgcgccgatc
     6781 gcagcatcga acagcgggcc gcaactgtcc ggctgcccct tgccgctgaa ccagagcctg
     6841 cgcgcctcct gcagcgccgc ctgctcatcg ctgcggcgcc gggattgcag ggcataacag
     6901 gccaattcgg catcctcgtc gagcaggcgc ggatattcgg catcgaacag ctgccattgc
     6961 tgcctgttgg ccagctgctt gagccattcg ccgcgcagac ggtcaaccat aggcgtgtct
     7021 tccggccgcg ccagaaagtc gcgcacggcc tgcggatcgg cctgttccag ccccagacgc
     7081 aactggtagt agctgatgta cacctccagc ggcgtcttct tgaagcgtgg cgcgagacga
     7141 tccagcttgg cccggtcacc ggcccggaag gcatcgtgag cagccagaaa cgcggcatcg
     7201 tcggtagccg ccatctccgg cggcaggtgt ttggatggct gtgcggccag tgcggcgggc
     7261 acgaaagcca gcaggaagta cagtatgaat ctcatgccta ttttcgaaaa atcaatcgcg
     7321 gcgagcatag cacatcggcc cttccttacg cccccgaggc ggcaggagag gccgcttttc
     7381 tgctaaagtg cgcgccatga aacagatgac cagcacccag ctttacctgc gcctgttagg
     7441 ctatgtcaaa ccctattggc gcgtgttcgc cgccgccatt ctcggcatgg cggtatccgc
     7501 cgccaccgaa ccgctgctgc ccgccctgct caaacccctg ctggacggca ccttcgtcaa
     7561 caaggacgat gcggtcatcc gctgggcccc cgtgctgctg ctgatcattt tcttcgtgcg
     7621 cggcatcgcc tccttcgtcg gcacctatgc gatccactgg gtcggcaacc ggctggtgat
     7681 ggacctgcgc aacaagatgt tcaacaagct gctggcgctg cccacgcgct tctacgacga
     7741 tcacgccacg ggcaacctga tctccaagct gaccttcgac gtgaccaacg tcaccgccgc
     7801 cgccaccagc gtggtgacca tcaccatccg cgattccatc atcgtcgtgg gcctgctggg
     7861 ctggctgttc tacctcaact ggcagctgac gctgctgtcg ctgctggtcg cgccggtcgt
     7921 ggcactggtc atcagcacca tcaaccgccg cctgcgcgcg accagccgcg attcgcagcg
     7981 cgcgatgggc gatgtgaccc aggtgatcga ggagagcgtg accgcacaca aggtggtcaa
     8041 gctgttcggc ggccagcgct acgaaagcga gcgcttccac gaacaggcca actgggtgcg
     8101 ccgtcacgcg gtgaaacagg ccgctgctgc gtcggccaac gtgcccatcg tgcagatggt
     8161 ggccgcgatc gcgctgtcca tcatcatcta tatcgccacc gaacagtcgc gcagcaacga
     8221 gatcaccgtg ggcgccttcc tgtcgttcgt caccgcgatg ctgatgctga cctcgccgtt
     8281 aaagcgcctg accggggtca gcgaattcat gcagcgcggc ctggccgcag ccgaaagcgt
     8341 gttcgagctg ctggacaccg acggcgaagt cgacaacggc aagatcgaaa tcggccgcgc
     8401 cagcggacat ctgtccttcg agaaggtggc gttctcctac cagatcgacg acaggctggc
     8461 cctgcgcgat gtcacgctgg acatcccggc gggacagacc gtcgcgctgg tcggcgcctc
     8521 gggcagcggc aagaccacgc tcgccaatct ggtgccgcgc ttctacacgc cgaccagtgg
     8581 gcgcatcacg ctggacggcc aggacattac cgaactgacg ctggccagcc tgcgcgccaa
     8641 catcgcgctg gtgagccagg aggtggtgct gttcaacgac accctctccg ccaacatcgc
     8701 ctacggccag atgcgcgaag tcccgcagac cgagatcgtc gcggcggcga ccgccgcgca
     8761 tgcgctggaa ttcatcaacg acatgccgca aggcttcgac accctgatcg gcgaaaaggg
     8821 cgtacgcctg tccggcggcc agcgccagcg catcgccatc gcccgcgcca tcctcaagga
     8881 tcccccgatc ctcatcctgg atgaagccac ctccgcgctc gacaccgaat cggagagcct
     8941 ggtccagcag gccctgttca acctc
//
LOCUS       DASUIV010000172         4979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_133577, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000172 DASUIV010000000
VERSION     DASUIV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133577"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VFW59_06375"
     CDS             <1..1726
                     /locus_tag="VFW59_06375"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5363874.1"
                     /translation="ASDGLNAALNVAFKGGAITGMLVVGLGLLGVAGYYTLLTQLGTS
                     TAEATHALVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTIIATMVLGGLLITGSPEAVIYPLVLGGVSIIAS
                     IIGTFFVTAREGGKIMNALYRGLIVSGVLAVLAFYPVTTWILGEGVMIDGKLVSSMDL
                     YLASLIGLVLTAAMVLITEYYTATEYSPVRHIAQASTTGHGTNVIAGLGVSMKSTAAP
                     VLAVCASIGGAYYLGGLYGIAIAATSMLSMAGIIVALDAYGPITDNAGGIAEMAGLDE
                     SIRNITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHAINAKAILLDPLAQAMT
                     FDLSNHMVIIGLFIGGMVPYLFAAMGMEAVGRAAGSVVVEVRRQFKEIPGIMAGTAKP
                     EYGTCVDMLTKAAIREMIVPSLLPVAVPLIVGYFLGAQALGGVLVGTIITGIFVAISM
                     TTGGGAWDNAKKYIEEGNYGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIV
                     ALMIVPLI"
     gene            2309..2491
                     /locus_tag="VFW59_06380"
     CDS             2309..2491
                     /locus_tag="VFW59_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363875.1"
                     /translation="MNNLFSMLPILLVGAALLMAGILINRGKKQSLRSAIAESLGWGL
                     GFLFSFSAEILLLANS"
     gene            complement(2519..3694)
                     /locus_tag="VFW59_06385"
     CDS             complement(2519..3694)
                     /locus_tag="VFW59_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5363876.1"
                     /translation="MTEQPTINWSEAGKACSARWRAESGSPPPRRVVVADDTMKADAA
                     YRLACEGTALLWRGDFQNARQLLTALARRIERKPRKPVLSKAEGPVLSKAEGASKLTA
                     EAFHLHRQAQSQRARVLSMLLLPLDGDYSIPLRRAPDARQACVEAYGDADGLPSVVSL
                     RELQGLIGAHEWRKKGVEIPALGARIHPYYGVFSPLRGEYIQLVNEAPLPSTELAFDI
                     GTGTGVLAAVLARRGVKQVVATDQDLRALACARENLERMALSGQVEVVQADLFPAGRA
                     PLIVCNPPWVPARPSSPIEYAIFDPDSRMLRGFLGGLAAHLSPGGEGWLILSDLAEHL
                     GLRRRDELLALFEAAGLQVLGRQEVKPHHSKAADDTDPLHAARAAEVTSLWRLAAKG"
     gene            complement(3755..4729)
                     /gene="rlmF"
                     /locus_tag="VFW59_06390"
     CDS             complement(3755..4729)
                     /gene="rlmF"
                     /locus_tag="VFW59_06390"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HEX5363877.1"
                     /translation="MAARPRRGTKGASADKNALHPRNPHRGRYDFRQLIACTPELAAF
                     VATNEHGIETIDFADPLAVKALNRALLQQQYGIGGWDIPAQYLCPPIPGRADYLHYLA
                     DLLAEGGVIPQGPSVRVLDIGVGANCVYPLIGHRSYGWQFVGTDIDPVALANAQRILD
                     ANGLSEVIGLRLQRSRSSVFAGVVESTERFDLAMCNPPFHASLEEACAGTQRKWRNLG
                     KPPAKNSAGLLNFGGQGAELCCEGGEEAFVSRMVDESASYAGNCLWFTTLISKSASLP
                     GVYRALKKTGVRESRTIDMAQGQKKSRIVAWTFLDESQQRAWRAARRK"
     gene            complement(4729..>4979)
                     /locus_tag="VFW59_06395"
     CDS             complement(4729..>4979)
                     /locus_tag="VFW59_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363878.1"
                     /translation="DHPVVDEIREVIEPAENPDGTRIVDLHVWRVGKESYACAVSIVT
                     HSETLSPEMLREQLRQHEEIVHTTIEIHRCACQACPAN"
BASE COUNT          956 a         1640 c         1441 g          942 t
ORIGIN      
        1 agccagcgac ggactgaatg ccgcattgaa cgtcgccttc aagggcggcg cgatcaccgg
       61 catgctggtg gtcggcctag ggctgctggg cgtggccggt tattacaccc tgctgacaca
      121 gctgggcacc tcgaccgccg aagcgaccca tgcgctggtc ggcctcgcgt tcggcggctc
      181 gctgatctcc atcttcgccc gtctcggcgg cggcatcttc accaagggtg cggacgtcgg
      241 cgccgatctg gtgggcaagg tcgaagccgg tatccccgag gacgacccgc gcaacccggc
      301 agtcatcgcc gataacgtcg gcgacaacgt cggcgactgc gccggcatgg cggcagacct
      361 gttcgagacc tacgccgtga ccatcatcgc caccatggtg ctcggcggcc tgctgatcac
      421 aggctcaccc gaggcggtga tctatccgct ggtactcgga ggcgtctcca tcatcgcctc
      481 catcatcggc accttcttcg tcaccgcacg cgagggcggc aagatcatga acgcgctgta
      541 tcgcggcctg atcgtatccg gcgtgctggc cgtgctcgcg ttctacccgg tgaccacctg
      601 gatactcggc gaaggcgtga tgatagacgg caagctggtc tcgtcgatgg atctgtatct
      661 ggcctcgctg atcggcctgg tactgaccgc agcgatggtg ctgatcaccg agtattacac
      721 cgccaccgaa tacagcccgg tgcgccacat cgctcaggct tccaccaccg gtcacggcac
      781 caacgtgatc gccggtctgg gcgtgtcgat gaaatcgacg gccgctccgg tactggccgt
      841 ctgcgcctcc atcggcggcg cctattatct gggcggcctc tatggcatcg ccatcgccgc
      901 gacttcgatg ctgtcgatgg cgggcatcat cgtcgcactc gatgcctacg gcccgatcac
      961 cgacaatgcc ggcggcatcg ccgaaatggc cggtctggat gaaagcatcc gcaacatcac
     1021 cgacccgctc gacgcggtgg gcaacaccac caaggcggta accaagggct acgcgatcgg
     1081 ctccgccggt ctggccgcac tggtgctgtt cgccgactat acccatgcga tcaacgccaa
     1141 ggcgattctg ctcgatccgc tcgctcaggc catgaccttc gacctgtcca accacatggt
     1201 catcatcggc ctgttcatcg gcggcatggt gccctacctg ttcgccgcga tgggcatgga
     1261 ggcagtcggt cgcgccgcag gatcggtggt ggtcgaagtg cgccgccagt tcaaggaaat
     1321 tcccggcatc atggctggca ccgccaaacc ggaatacggc acctgcgtgg acatgctgac
     1381 caaggccgcg atccgcgaga tgatcgtgcc gtcgctgctg ccggtcgcgg taccgttgat
     1441 cgtcggctat ttcctgggcg cacaggcgct gggcggcgtg ctcgtcggca cgatcatcac
     1501 cggcatcttc gtcgcgatct cgatgaccac cggcggcggc gcatgggata acgccaagaa
     1561 atacatcgaa gaaggtaact atggcggcaa gggctcggat gcgcacaagg ccgccgtcac
     1621 cggcgacacc gtgggcgacc cttacaagga taccgcaggc cctgcggtga atccgctgat
     1681 caagatcatc aacatcgtcg cgctgatgat cgtgccgctg atctgatgcc tggctgagcg
     1741 cacaaagaaa accgggcata tgcccggttt tttatgtcgc tcactatccg aagtagtgcc
     1801 gcgaaccagt cagacaatcc atctgacggg taacagacta cgccgtcgta tcgactattc
     1861 gcccaatgtg aacatcgtat cccggcacca catgttgttc gtccgcagga gacttctttt
     1921 ctgcggccgt gcggatgctc ttttgcgccg acctgtccct gccctgactg gatcttcgtc
     1981 cctctggcct gtgctcgata tattgcacgc tacctatcaa gtccatgatt tcctcctgtg
     2041 tcatcaataa catcggcaaa ctcgctggat tctttatgcc cgcttgcatg cacccgactg
     2101 ccccggccat tcggcaccgc cccggcaggt caaagcacac tcggcaaaat cccgtctcga
     2161 aagcttcaca tttacatcac aaaaatgaaa cagcgcctca ctaaagtggc ttcaggaaaa
     2221 agcgaggccg gtcgtcgcat atgacacagc agctcaggac gagcaccagg ccaaactgat
     2281 aaccagaacc aatcgttggg agacgctgat gaataattta ttttccatgc tgccgatact
     2341 gctcgttgga gcggcgctgc tgatggcggg catcctgatc aaccggggca agaaacagag
     2401 tctgcgcagc gcaatcgcgg aaagtctggg atgggggctg ggcttcctgt tttcattcag
     2461 cgccgagatc ctgctgctgg cgaatagttg aatcgagacc ggctacccgt ttggggcctc
     2521 agcccttggc tgccagccgc cacaaagagg tcacttctgc cgcccgcgcc gcgtgcaacg
     2581 ggtcggtgtc gtcggcagcc ttggaatgat gcggcttcac ttcctgtctg cccagaacct
     2641 gcaaacccgc agcctcgaat aacgccagca gttcatcgcg gcggcgcagc cccagatgct
     2701 cggccaagtc agacaggatc agccagcctt cgcctcccgg cgacagatgc gcggccagtc
     2761 cgccgaggaa gccgcgcagc atgcggctat cgggatcgaa gatcgcatac tcgatgggcg
     2821 aactcgggcg cgcaggcacc cagggcgggt tgcacacgat caacggtgcg cggcctgcag
     2881 ggaacagatc ggcctgtacc acctcgacct gtccgctcaa tgccatgcgc tcaaggttct
     2941 cgcgtgcgca ggccagcgcg cgcaagtcct gatcggtcgc caccacctgt ttgacgccgc
     3001 gacgcgccag caccgcagcc agcacaccgg tgccggtacc gatatcgaaa gccaattcgg
     3061 tcgagggcaa cggtgcctcg ttcaccagtt ggatatattc gccgcgcaac ggcgagaaca
     3121 caccgtaata aggatggatg cgcgcaccca gcgccggtat ttcgacgccc ttcttgcgcc
     3181 actcgtgcgc gccgatcaat ccctgcagtt cacgcaacga aacgaccgaa ggcagtccgt
     3241 cagcatcgcc gtaggcttcc acacaggctt gccgggcatc cggcgcacgc cgcaacggaa
     3301 tgctgtaatc gccgtccagc ggcagcaaca acatggacag cacccgcgcg cgttgcgatt
     3361 gcgcctgacg atgcagatga aacgcctcgg ctgtcagctt cgacgcccct tcggccttgc
     3421 tcaggacagg cccttcggct ttgctcagga caggcttgcg cggcttgcgc tcgatgcgcc
     3481 ttgccagcgc ggtcagcaac tggcgcgcat tctgaaagtc accgcgccac aacagcgcgg
     3541 tgccctcgca ggcgagacga taggcggcat ccgccttcat cgtatcgtcg gccacgacca
     3601 cgcgtctggg cggcggcgaa ccgctctcgg cgcgccaacg cgcagaacaa gccttgcccg
     3661 cctcgctcca gttgatggtc ggttgttcag tcatcgcaaa atgcccctga tgttcatggt
     3721 tgtgtacatc cgaattcctc tgattgatcc ggcactattt ccggcgcgcc gcccgccatg
     3781 cgcgctgttg gctctcatcg agaaaagtcc aggccacgat gcggcttttt ttctgcccct
     3841 gcgccatgtc gatggttctg ctctcccgca ccccggtctt tttcaatgcg cgataaacac
     3901 ccggcagact ggccgacttc gagatcagcg tggtgaacca gagacagttg cccgcatagc
     3961 tcgcgctctc atccaccata cggctcacga aggcttcttc gccgccctcg cagcacaact
     4021 cggccccctg cccgccgaag ttcagcagcc ctgcagaatt tttggccggc ggtttaccga
     4081 gattgcgcca tttgcgctgc gtgcccgcgc aggcctcctc cagcgacgca tgaaacggcg
     4141 gattgcacat cgcaagatcg aaccgttcgg tcgactctac cacgcccgcg aagactgagg
     4201 atcgggagcg ttgcaatcgt agcccgatga cttcgctcaa accgttcgca tccagaatgc
     4261 gttgcgcgtt ggcgagcgcc accgggtcga tgtcggttcc gacaaactgc caaccatagg
     4321 aacggtgacc gatcagcgga tagacacagt tggcaccgac accgatatcg agcacccgca
     4381 ccgaagggcc ctgcggaatg acaccgccct ccgccaacag gtcggcgaga taatgcagat
     4441 agtccgcgcg ccccgggatc ggcgggcaaa gatactgcgc cgggatatcc cagccaccaa
     4501 taccatactg ttgctgcaac agcgcacggt tgagcgcctt gacggccaac gggtcggcga
     4561 aatcgatcgt ctcgataccg tgctcgttcg tcgccacgaa tgccgccagc tcgggggtgc
     4621 aggcgatgag ttgcctgaag tcgtaacgtc cccgatgggg attgcgcgga tgcagcgcat
     4681 tcttatcggc ggaagccccc tttgtccccc ggcgtggtct ggccgccatc agttcgcggg
     4741 gcaagcttgg catgcacagc gatgaatctc gatcgtcgtg tggacgattt cctcgtgctg
     4801 ccgcaactgc tcacgaagca tctcgggcga gagcgtttcg ctgtgcgtca cgatgctgac
     4861 cgcgcaggca taggattctt tcccgacacg ccacacatgc agatcgacga ttctggtccc
     4921 atccggattt tcagcaggct cgatgacttc acgaatctca tccaccacgg gatgatcca
//
LOCUS       DASUIV010000173         8599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_40754, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000173 DASUIV010000000
VERSION     DASUIV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40754"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VFW59_06400"
     CDS             <1..1212
                     /locus_tag="VFW59_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX5363879.1"
                     /translation="GSIFYSRNGQFQVDKNGFIVNSQGHKVSGYLPNAAGMIVAAQPV
                     PLQVNAADLTPKTSVNVAVGANLDSRSLGAGVGGAVPVTPVFNPLDPTSYNNSTSMTV
                     YDSLGVNHVASLYFQRQPIAPTTTAAAIAAAANSTTLTSAAGMAVGNTITIAGAGPLG
                     VPLTATISAIAGNVVSFSPSTSTAVAAGAAVTSNAASSNWNTYLTVDSNLVPAPVPPA
                     APTPLTTLTFDALGKLTGPAVPQLGVVTSAAFAPAGAALQPLTFNFSQTTQYGGNFGV
                     NTLTQDGYASGRLNGFSTSQDGTVLGRYSNGQSRPLGQVLLANFTNPQGLQPVGNNEW
                     AESAASGQPLIGTPGSSSLGVLQSSAVEDSNVDLTAELVNMITAQRVYQANAQTIKAQ
                     DQVLQTLVNLR"
     gene            1264..2007
                     /gene="flgF"
                     /locus_tag="VFW59_06405"
     CDS             1264..2007
                     /gene="flgF"
                     /locus_tag="VFW59_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEX5363880.1"
                     /translation="MDKLIYTAMTGASHVLQQQAAVSENLANASTPGFRATLNTFRAV
                     PLVGEGLPTRTFVVDSTAGADFTPGAFQQTGRSLDVAVNGAGWVVVQGADGREAYTRN
                     GSFQVSSSGILQTRTGLNVLGDGGPITIPPDTEVTFAKDGTISTVPSGSQATSVIAVG
                     RLKLVNPPEAQLERGGDGLFRMKDGTTAAADAKVEVAPGSLETSNVNTVEAMVTMISL
                     ARKFDMQMKMLQSADNNARQASQIFALNG"
     gene            2056..2838
                     /gene="flgG"
                     /locus_tag="VFW59_06410"
     CDS             2056..2838
                     /gene="flgG"
                     /locus_tag="VFW59_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX5363881.1"
                     /translation="MIRSLWISKTGLDAQQTQMDVVANNLANVSTNGFKRSRAVFEDL
                     LYQTIRQPGAQSSQQTMIPSGLQIGTGVRPVAAERIHTQGNLQQTGNQLDVAIQGAGF
                     FQILMPDGSTAYTRDGSFQRDSQGQMVTAGGFAVQPAITLPANSINVSIGRDGVITVT
                     QPGVVAPVQVGSLQLATFINPSGLMSQGENLYQETASSGTPSTNVPGTNGAGLLNQSY
                     VETSNVNVVEEMVNMIQTQRAYEINSKAIQTSDQMLQKLSQM"
     gene            2874..3608
                     /locus_tag="VFW59_06415"
     CDS             2874..3608
                     /locus_tag="VFW59_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX5363882.1"
                     /translation="MQMLGRVGLLVVALLGLAGCASNPPPSIVHQPMTAKPVEYKPVQ
                     YADGAIFHAGANERPLFEDRRARNVGDILTINIVEKTTGNRKSSNSSANTNSIVAKAP
                     TIGSLGGNVVTPAAGLISPFVQKVLNLFSVTGSGASSTSGSSAGAASEDLTGTITVTV
                     IEALPNGNLLVSGEKQVGLNSSDEYIRFSGVVNPTTITGANTVQSTQVADARLEYRTA
                     GAMRQVVNDAQTLGFLGRLFMSVLPF"
     gene            3690..4787
                     /locus_tag="VFW59_06420"
     CDS             3690..4787
                     /locus_tag="VFW59_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX5363883.1"
                     /translation="MKRIFSIMSVLMLLSPFAASAERIKDMASIQGVRDNQLIGYGIV
                     VGLDGSGDQTTQTPFTVQSIISMLSQMGVNLPAGTSLQLKNVAAVMVTATLPPFSKPG
                     QTIDVTASSIGNAKSLRGGTLLMTPLKGANGQVYAMAQGTVLVGGVGASSGGASVQVN
                     HLSVGRVPAGATVEREVPTVLGQGEFIHLELHANDFTTASRIVEVINSQVGPDLAAAL
                     DGRMIEVRAPAGNQRVAFIAKIENLEVNPAQGIAKVIINARTGSVVMNQAVTLSACAV
                     AHGNLTVVINTDSAVSQPNAKSDGKTVQTDKTSIDVKADKGGLVQLQAGVNLSEVVKA
                     LNSMGATPQDLLAILQAMKSAGALRAELEII"
     gene            4787..5665
                     /gene="flgJ"
                     /locus_tag="VFW59_06425"
     CDS             4787..5665
                     /gene="flgJ"
                     /locus_tag="VFW59_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEX5363884.1"
                     /translation="MVNRLDISSKLALDAQSLDQLRAQARQSPDQALKAAAQQFESVF
                     LNMMLKSMREATPQDGMMESDQTRMFTGMLDQQLAQNMSSRGVGLADIMVQQLSRSLP
                     GQAAAVESGGVQVPLDLQSTATPASRGISALPSAYSENAQQDFVNRMLPHATQASRAS
                     GVPAQLMLGQAALESGWGKREIRMADGSNSFNLFGIKATAGWNGKVAEVMTTEYRDGV
                     AHKQIERFRAYSSYAEAFQDHARLIGSNPRYAEVLQQAGNPAGMAVAIQRAGYATDPN
                     YADKLVRVMGMMKTEV"
     gene            5758..7686
                     /gene="flgK"
                     /locus_tag="VFW59_06430"
     CDS             5758..7686
                     /gene="flgK"
                     /locus_tag="VFW59_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX5363885.1"
                     /translation="MGSNLFTSSLSGMNAAQYGLTTTQHNISNANTPGFTRQETVVSS
                     RPGSQFGGGFIGQGVDVTGVKRIYDQFLSTQVLQEQSQATYLTTYYTALKQIDNLVAD
                     PTAGAAPAMRDFFAATNGVSNSPESIPARQTMLSNAQFAVNRFQAIDQRLTDIANGLS
                     AQLTSSVDLVNSYSKQIAALNGSIKRAVATGNGQQPNDLLDQRDQLITKLNQEVKTTT
                     LKQADGTMNVFIGTGQTLVVDEQAMSLRVVRSATDPSKVDVAYQNNNSSVVMQQSSLQ
                     GGNLGAFLGFRDQTLEPMRNELGRVALSLAMSVNQQNQAGLDLRGQPGKALFNAAAPR
                     VDMGGLNQGTATVTASIANLAAITTSDYQLKVNGGGSYTMVRLSDNFVTNITPPAVMP
                     QTIDGISVDISAGAIAGDSFLIRPTANAARDLAVMTNDPASIAAAAPIRGNATLTNLG
                     TGVIGAGSVNMPLPLDPNLQAPVTITFNAGNTYTVSGTLSLPAVSPVTLPYTPGTPIN
                     LSYNGWTTQITGTPSTNDTFTVSANTNATGDSRNALLMANFQTTGVLDGGTTGIQAAY
                     SQLVGRVGAKTNEFAVTSVAQTNMVAETIKQQQSTSGVNLDEEAANLLRFQRAYEASA
                     KALQVSNTMFDALLALGK"
     gene            7694..>8599
                     /gene="flgL"
                     /locus_tag="VFW59_06435"
     CDS             7694..>8599
                     /gene="flgL"
                     /locus_tag="VFW59_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEX5363886.1"
                     /translation="MRISSNTMYDTSVAAMTNQQARLLQTQQQITSGRRILTAADDPV
                     AAARALDITQSDAMNTQYASNRDAARHSLSLTEANLQGVTSLLQDVKTAVVNAGNGAF
                     TVSDRRSIATELNGRLQELIGLANSTDGIGNYLFSGFQTRTPPFANTPGGVAYFGDDG
                     QRMVQVSQARQLSSTNTGAEVFMRIKDGNGTFTTGAAATNTGTGVISIGSLGNPPPTA
                     AQAGNSYQLAFTVSPPATPGLPDVITYTVTGTDALGAPIPSASLPNPGSLPVPNTASY
                     VSGQAISFNGIQFDIQGVPKTGDTFT"
BASE COUNT         1799 a         2313 c         2713 g         1774 t
ORIGIN      
        1 ggctctattt tttacagccg caacggccag ttccaggtgg acaagaacgg tttcatcgtc
       61 aatagtcagg ggcacaaggt ctccggctat ctgccgaatg ccgccgggat gatcgttgca
      121 gcccagccgg tgccgttgca ggtcaatgcg gccgatctga cgcccaagac ctcggtcaat
      181 gtggcggtcg gcgcgaatct ggattcgcgt tcgctgggtg cgggggtggg cggtgcagtg
      241 ccggttacgc ctgtatttaa tccgctggat ccgacgtcgt ataacaattc gacctcgatg
      301 acggtgtatg acagtctggg cgtcaatcat gtggcatcgc tgtactttca gcgtcagccg
      361 atagcgccta cgacgactgc tgcagccatc gctgctgctg cgaattcgac gacattgacg
      421 tcggcggccg ggatggcagt cggcaacacg attaccattg ccggagccgg cccgctgggc
      481 gtgccgctga ctgcgaccat ctcggcaatc gcgggtaacg tggtgtcgtt ctcgccatcg
      541 acgagtacgg ctgttgctgc tggcgcggcg gttaccagca atgccgcttc gtcgaactgg
      601 aacacctatc tgaccgtgga cagcaatctg gtgcctgcgc cagttccgcc agcagcgcct
      661 actccgttga ccacgttgac cttcgatgcg ctggggaaat tgacggggcc agccgtgccg
      721 caactcggtg tggtgacgtc tgctgcattc gccccggccg gggctgctct tcagccgctg
      781 actttcaatt tctcccaaac cacgcaatac ggcggaaatt tcggtgtaaa caccctgacg
      841 caggatggct atgcgtcggg ccgcttgaat ggtttcagca ccagtcagga tggcacggtg
      901 ctggggcgat actccaacgg ccagtcgcgg ccattggggc aggtgctgct ggcgaacttc
      961 accaacccgc agggcttgca gccggtcggc aacaacgagt gggccgagtc ggcagcctcg
     1021 ggccagccgc tgatcggcac gccgggttcc agcagtctgg gagtgttgca gtcttctgcg
     1081 gtcgaagatt ccaacgtgga cttgacggca gagctggtga acatgatcac cgcgcagcgc
     1141 gtctatcagg cgaatgccca gaccatcaag gcgcaggatc aagtgctgca aacactggtg
     1201 aatctgcggt aatagttagg caggggcacg gcgcgtcgtg cccctgcagc ccaggagaac
     1261 gcaatggata aattgatcta taccgcaatg accggcgctt cgcatgtgct gcagcagcag
     1321 gcggcggtgt cggagaatct ggcgaatgcg agtaccccgg gtttccgggc tacattgaac
     1381 acctttcgtg ccgtgccgct ggtgggtgag ggcttgccga cacgaacctt cgtggtggat
     1441 tccacggcgg gggcggactt tacgccgggg gcttttcagc agaccgggcg tagtctggat
     1501 gtggcggtga atggcgcagg ctgggtcgtg gtgcaggggg cggacgggcg cgaggcttat
     1561 acgcgcaacg gcagttttca ggtgtcgtcg agcggcatat tgcagactcg aaccggtttg
     1621 aatgtgctgg gcgatggcgg ccccatcact attccgccgg atactgaagt gaccttcgcc
     1681 aaggacggta cgatttcgac ggtgccaagc ggttctcagg cgacctcggt gatcgcagta
     1741 ggccgactga agctggtcaa tccgcctgag gcccaactcg aacgcggagg cgatggcctg
     1801 tttcgcatga aggatggcac gacagcggcg gcggatgcca aggtggaggt ggcgccgggc
     1861 agtctggaaa ccagtaacgt aaataccgta gaagcgatgg ttaccatgat ctcgctggcg
     1921 cgcaagttcg atatgcagat gaaaatgctg cagagcgcag acaataacgc aagacaggcc
     1981 agccagatat tcgcgctgaa tggctagtag gggcgcggcg cgcctctgcg tttctttatt
     2041 aaaaggataa gaatcatgat acgttctctg tggatttcca agacgggtct ggatgctcag
     2101 cagacccaga tggatgtggt cgccaacaat ctggctaacg tcagcaccaa cggcttcaag
     2161 cgttcgcgtg cggtatttga agatttgctg tatcagacca tacgtcagcc cggggcgcag
     2221 tcgtctcaac agaccatgat tccatcggga ttgcagatcg gtaccggtgt tcgtccggtg
     2281 gccgctgaac ggattcatac tcagggcaac cttcagcaga ccggaaatca actggatgtt
     2341 gcgattcagg gggcgggttt ctttcagatt ctgatgccgg atggttcgac ggcatatacc
     2401 cgcgacggct cttttcagcg cgatagtcag gggcagatgg ttaccgccgg tggcttcgcg
     2461 gtgcagccgg cgatcacgct gcctgcaaac agcatcaatg tgtcgatcgg tcgcgatggc
     2521 gtgattacgg tcacccagcc gggcgtggtg gcgccggtgc aagtgggcag cctgcaactg
     2581 gcgaccttca tcaatccgtc tggcttgatg agtcagggtg agaatctgta tcaggagacc
     2641 gcctcgtccg ggacgccgag caccaatgtg cccgggacga acggtgcggg tctgttgaac
     2701 caaagctatg tggagacttc taacgtcaac gtagtggagg agatggtgaa tatgatccag
     2761 acgcaacgcg cttatgagat caattcgaag gcgattcaga cctcggatca gatgctgcag
     2821 aagctgtcgc agatgtaatg cttaagtgag tggtttaatg tcaggcgtgg ggtatgcaaa
     2881 tgttgggtag ggtcggtttg ttggtcgtgg cgttgcttgg tctggctggt tgtgccagta
     2941 atccgccgcc gagtatcgtg catcagccga tgacagccaa gccggtcgag tacaagcccg
     3001 tgcaatatgc ggatggagcg atattccatg cgggcgcgaa cgagcggccc ctgttcgagg
     3061 accggcgcgc ccgcaacgtg ggtgacattc tgaccatcaa tatcgtggaa aaaaccacgg
     3121 gcaaccgcaa gtccagcaat agttctgcca ataccaattc gatcgtcgcc aaggccccga
     3181 ccattggttc gctgggcggg aatgtcgtga cgccagcggc gggcctgatc tctccctttg
     3241 ttcagaaagt gttgaatttg ttctcggtga caggttccgg agcaagcagt accagcggtt
     3301 ccagtgcggg ggcggcgagt gaagatttga ccggaaccat cacggtgacg gtgatcgagg
     3361 cattgccgaa cgggaacttg ctggtgagcg gcgagaagca ggtggggctc aacagcagcg
     3421 acgaatatat tcgtttctca ggcgtggtta atccgactac aatcacggga gcaaacactg
     3481 tgcaatcgac acaagttgcg gatgcccgcc tcgaatatcg gacggcgggc gcgatgcgtc
     3541 aggtcgtgaa cgatgctcag accctgggtt ttctggggag gctcttcatg tcggtgttgc
     3601 cattctaggg cggatggatt gagggttgag caatgggctg tcggctctcc gctccccgat
     3661 tatccttggc ctaattgagg agcagggtaa tgaagcgaat tttttctatc atgtctgtgc
     3721 tgatgctgct gtcgccattt gcggcttctg cagagcgcat caaggacatg gccagcatcc
     3781 agggggtgcg tgacaaccag ttgatcggct acggtatcgt ggtggggctg gatggcagtg
     3841 gtgaccagac cacgcagacc ccttttaccg tgcagagcat catcagcatg ctatcccaga
     3901 tgggcgtgaa tctgcccgca ggtaccagtc tgcaactcaa gaacgtggcg gcggtcatgg
     3961 tgacggctac gttgccgccc ttttccaagc caggtcaaac cattgatgtc accgcttctt
     4021 cgataggcaa tgcgaagagc ctgcgcggtg gcacgctgtt gatgacgccc ttgaaggggg
     4081 cgaatgggca ggtctatgcg atggcgcagg gcactgttct ggtcggtggg gtgggcgctt
     4141 cttccggtgg tgcctcggtg caggtgaatc atttgagtgt cgggcgtgtg ccagccgggg
     4201 cgacggtcga gcgcgaggtg cctaccgtgc tgggccaagg ggagtttatt catcttgaat
     4261 tgcatgccaa cgatttcacc acggcttcgc gcatcgttga ggtgatcaat tctcaggttg
     4321 gtcccgatct ggctgcggca ctggatggcc ggatgatcga ggtgcgagca cctgcgggca
     4381 atcaacgggt tgctttcatt gccaaaatag aaaatctgga agtcaatccg gcacagggca
     4441 tagccaaggt catcatcaat gcccggacgg gttcggtcgt gatgaatcag gcggtgacat
     4501 tgagcgcttg tgccgtggcg cacggcaatc tgacggtggt gatcaatacg gacagcgcgg
     4561 tgagccagcc gaatgcgaaa tcggacggta aaacggtgca aaccgacaag acctcgatcg
     4621 atgtcaaggc ggacaagggc ggtttggtgc agcttcaggc cggcgtgaat ctcagcgagg
     4681 tggtcaaggc gctcaattcg atgggggcaa ccccgcagga tctgttggct attctgcaag
     4741 cgatgaagtc agccggtgcc ttgcgggctg agctggagat tatctgatgg tcaatcgtct
     4801 ggatatctct tccaagctgg cgctcgatgc acagagtctg gatcaattgc gtgcgcaggc
     4861 aaggcaatcg cccgaccagg cgctcaaggc ggcagcacag caattcgaat cggtatttct
     4921 gaacatgatg ctgaaaagca tgcgtgaggc gacaccgcag gacggcatga tggagagcga
     4981 tcagacgcgg atgttcaccg gcatgctgga tcagcagctg gcgcagaaca tgtccagccg
     5041 tggtgtgggg ctggccgata tcatggttca gcagctcagt cgcagtctcc cggggcaagc
     5101 tgctgccgtc gagtcgggag gggttcaggt tcctctggat ctgcagtcga ccgctacgcc
     5161 agcctcgcgc ggaatcagtg ccttgccgtc tgcatacagc gagaatgccc agcaggactt
     5221 tgtgaaccgg atgctgccac acgctacgca ggcgagtcgg gcaagcggtg tgccagcaca
     5281 attgatgctc ggacaggctg cgctggaaag tggctggggc aagcgcgaga tccgcatggc
     5341 tgatggcagc aatagcttca atctttttgg aatcaaggcc actgcaggtt ggaacggcaa
     5401 ggttgccgag gtgatgacga ccgagtatcg cgacggggtt gcgcacaagc agatcgaacg
     5461 gttccgagct tattcttctt atgccgaagc atttcaggac cacgcgaggc tgattggttc
     5521 caatccgcgc tatgcggaag tattgcagca ggcgggtaat cctgccggga tggccgtcgc
     5581 gatccagcgg gcgggctacg cgaccgatcc gaactatgcg gacaaactgg tgcgagtgat
     5641 ggggatgatg aagacggagg tctgatcatg cctgtttcat cttcgtcggt ataaagtatt
     5701 cgctgctggt gtcgataaca cagctatgta cgcctgttgg cgaaagaagg ggaagtcatg
     5761 ggaagcaatt tgtttacctc atcattgagc ggcatgaatg cggctcagta cggtctgacg
     5821 accacccagc acaatatttc caatgcgaat acgccgggct tcacccgtca ggaaacggtg
     5881 gttagttccc ggccgggctc gcagtttggc ggaggtttca tcggccaggg tgtcgatgtg
     5941 acaggggtca aacgcatcta tgaccagttc ctgtcaacgc aggtgttgca ggaacagtct
     6001 caggccactt acctcacgac ctactacacg gcgctcaagc agatagacaa tctggttgcc
     6061 gatccgaccg caggggccgc gcccgcgatg cgggattttt tcgccgccac gaatggtgtt
     6121 tccaatagtc cggaatccat ccccgcgcgc cagaccatgt tgagcaatgc gcagttcgcc
     6181 gtgaaccggt ttcaggcgat tgaccagcgt ctgaccgata ttgccaacgg gctgagtgct
     6241 cagctcacca gcagcgtgga tttggtcaac agttattcga aacagatcgc ggccttgaat
     6301 ggcagtatca aacgtgccgt cgcgacgggc aacggacagc aacccaacga tctgctcgat
     6361 cagcgcgacc agctgattac gaagctgaat caggaagtaa agaccaccac gctgaagcaa
     6421 gcggacggga cgatgaacgt gtttatcgga accggtcaga cgctggtggt tgacgaacag
     6481 gcgatgtctt tgcgggtggt gaggtctgcg actgacccca gcaaggtgga tgtcgcctat
     6541 cagaacaata attcgtccgt ggtcatgcag cagagcagtc ttcagggcgg gaatcttggg
     6601 gcctttctgg gcttccgcga ccagacgctg gagccgatgc gcaatgagct ggggcgggtg
     6661 gcgctgagtc tggcgatgag cgtcaaccag caaaatcagg cgggtctgga tttgcgcggg
     6721 cagccgggca aggctctctt caacgcggcg gcgccgcgcg tcgatatggg cggcctgaat
     6781 cagggcaccg caactgtcac cgcgagtatc gccaatctgg ctgcgatcac aaccagcgat
     6841 taccagctta aggtgaatgg tggcggcagt tatacgatgg tgcgtttatc cgacaatttc
     6901 gtgaccaaca tcaccccccc cgccgtgatg ccgcagacga tagatggcat atccgtcgat
     6961 atttcggctg gcgcgatcgc cggggacagc ttcctgattc ggcccactgc gaatgccgcg
     7021 cgcgacctcg cggtgatgac caacgatccg gcttcgattg cggcagcggc gccgatacgc
     7081 gggaatgcaa ctctgacgaa tctaggaacc ggagttattg gtgctggatc ggtgaacatg
     7141 cctttgccgc tggatcccaa tctgcaggcg ccagtcacga tcaccttcaa tgccgggaat
     7201 acctataccg tatcggggac gctttcactg cctgccgtta gcccagtgac actgccttat
     7261 acgcccggaa cgccaatcaa cctgtcctac aacggctgga cgacacaaat taccggtaca
     7321 ccatcgacca acgatacttt tacggtcagc gcgaatacca atgccaccgg tgatagccgc
     7381 aatgccttgc tgatggcgaa cttccaaacc acgggtgtgc tggacggcgg cacgacgggc
     7441 attcaggcgg catacagcca gttggtgggg cgggtcggtg ccaagaccaa cgagtttgcg
     7501 gtgaccagcg tggcccagac caatatggtg gccgagacga tcaaacagca acagtccacg
     7561 tcgggggtca acctcgatga ggaagcggca aatctattgc gttttcagcg cgcgtacgag
     7621 gcctcggcca aggcgctgca agtttccaat accatgttcg atgcgctatt ggcgctagga
     7681 aagtaggtgt gaaatgcgaa taagctctaa taccatgtat gacaccagcg tggctgcgat
     7741 gaccaatcag caagcacgcc tcttgcagac gcaacaacag atcacttccg gcaggcgcat
     7801 cctgacggct gcggacgatc ccgttgcggc cgccagagcg ctggatatca ctcagtccga
     7861 tgcgatgaat acccagtatg cgtcgaatcg tgacgcggcc agacactctt tatctcttac
     7921 agaggccaac ttgcagggcg tgaccagcct gttgcaggat gtgaagaccg cagtggtaaa
     7981 cgcgggcaat ggtgcattca ccgtcagcga tcgcagatcc attgcgacgg agttgaatgg
     8041 ccggttgcag gagctgatcg gcttggcaaa cagcaccgac ggtatcggca attatctgtt
     8101 ctccggcttc cagacccgca ccccgccctt cgccaatact cccggtggcg tagcgtattt
     8161 tggggacgat gggcagcgca tggtgcaggt gagtcaggct cgtcaattgt cgtccaccaa
     8221 tacgggcgca gaagtgttca tgcgcatcaa ggatggtaac ggtacgttca ccaccggcgc
     8281 tgcggcgacc aataccggta cgggggtgat cagcatcggc tccttgggca acccgccccc
     8341 cacggctgct caggccggga atagttacca gctggccttt accgtgtcgc ctccggctac
     8401 cccgggtttg ccggatgtga tcacctatac tgttacggga acggatgctc ttggcgcgcc
     8461 gattccttca gccagtctac ccaatccagg gagcctgcca gtgcccaata cggcttctta
     8521 cgtaagtggt caggcgatca gtttcaatgg catccagttc gatattcagg gcgtgcccaa
     8581 aactggcgac accttcacg
//
LOCUS       DASUIV010000174         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1157572, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000174 DASUIV010000000
VERSION     DASUIV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157572"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1033
                     /locus_tag="VFW59_06440"
     CDS             293..1033
                     /locus_tag="VFW59_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5363887.1"
                     /translation="MLQLKTVDKKLSGGSLKNLPELAWIHDERGAFAAAANTAREQEK
                     TRVARELHDELAQPLTALKMDTIWVRDNFVLAPEAAAARLGEMVEMLDRTIAATRRIA
                     SDLRPLLLDDLGLVPAVEWLVSKFTQRGGVACTLAVDEALELQEPCATAVFRIIQESL
                     ANVAKHAGASEVAVTIEKLRGAVTLSVRDNGCGFSPAAPRRPQSLGVLGLRERAQLLK
                     GTITIESAPGQGTCIQVSIPLPQASASR"
     gene            1030..1674
                     /locus_tag="VFW59_06445"
     CDS             1030..1674
                     /locus_tag="VFW59_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5363888.1"
                     /translation="MIRIVIADDHAIVREGLKRIIALADDMEVVGEAADGTEVMQRVR
                     ESIFEVLVLDLSMPGRSGMELIKLVHAERPKLRILVLSMHQELQYAVRAIKAGAGGYL
                     TKESAPDQLVQALRRIAAGGAYISAEVAEQLALGAMPGGVSAAHETLSDREFEVFSLI
                     VAGNSVTNIAARLNLSVKTISTHKTNLMHKMGLQNQSELIRYAIKNEVGDALDH"
     gene            1769..2611
                     /locus_tag="VFW59_06450"
     CDS             1769..2611
                     /locus_tag="VFW59_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5363889.1"
                     /translation="MKASLVQMLVHLDASPQVAQRLTAARQIAQAHGAAVTALYAVTP
                     NFVELPFVPELGQNVAADLREIDDELRASARATYEQSLTDSSVYMAWAEIRDDPVISL
                     FARQAFFADLLVLGQHDPSGKPSSGVPADFAESVLVASGKPALILPYAGMLPAVIGET
                     AVIAWKPTREAALAVSAAMPLLQRARRVHVLAWRGEEMAVDSKLLSLDGYLKLHGVDA
                     VWHSQGDEPPALGELLLSRACDLDADLLVMGCYGHSRAREWILGGTSRTVLRSMTLPV
                     LMAH"
     gene            2764..>2952
                     /locus_tag="VFW59_06455"
     CDS             2764..>2952
                     /locus_tag="VFW59_06455"
                     /inference="COORDINATES: protein motif:HMM:NF021621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX5363890.1"
                     /translation="MMKPDWMLIANASEARLLRREQGNLLVVLENFEHPESRSKVSDQ
                     VDDKMGWEKSDRGFGGSAY"
BASE COUNT          585 a          799 c          955 g          613 t
ORIGIN      
        1 gagatatttc cgtaggaata ctcttgtgtt tgccctattg ttcaattgtg ccgggggaga
       61 agtcagaatc ttccgcatca gtcagcgcaa gccaatagaa gaaatacatc aagcgatgga
      121 agtctgaagt tactatgcga agctgcaaca aagagtttct ggagggttta tggaaagttt
      181 tagtgggtct agcgccacag ccagttcgcc gatcagtttt ggggctcgtc cagcagggca
      241 ttactttttt ggaccgcgcc gaagcggcga aaattcacca atggatgcgg cgatgcttca
      301 gctcaagaca gttgataaaa agctttctgg tggatcgctg aaaaacttgc ccgagcttgc
      361 ctggatccat gacgaacgcg gcgccttcgc ggcggcggcc aacaccgccc gcgaacaaga
      421 aaaaacccgc gtggcgcgtg agttgcacga cgagcttgcc caacccctga cagcgttgaa
      481 gatggacacg atatgggtac gcgacaactt tgtgcttgcg cccgaggccg cagcggccag
      541 gctgggcgaa atggtggaga tgctggaccg tacgattgca gccacccgcc gcattgcgtc
      601 agacttgcgg ccgctgttgc tggatgattt ggggctggta cccgccgtcg aatggctggt
      661 gagtaaattc acccagcgcg gcggtgttgc ctgcacgctt gcggtggatg aagcgctgga
      721 actgcaggaa ccgtgcgcga cggcggtgtt ccggatcatt caggagtcac tggccaacgt
      781 cgccaagcac gctggtgcgt cagaggtcgc cgtgaccatc gaaaagctgc ggggtgccgt
      841 cacgttgtct gtgcgggaca acggctgtgg attttccccg gcagcgccgc gaaggccgca
      901 gtctctgggc gtcctgggct tgcgcgagcg ggcgcagttg ctcaagggaa cgatcactat
      961 cgagagcgcg ccgggtcaag ggacatgcat ccaggtgtct attccgttac cgcaggcgag
     1021 cgcatcgcga tgatcaggat cgtgatcgcc gatgaccatg ccatcgtcag ggaagggctc
     1081 aagcgcatta ttgcgttggc cgatgacatg gaggttgtgg gtgaggcagc tgacggaacg
     1141 gaagtcatgc agcgcgtgcg tgaatccatt ttcgaggttc tggtactgga tctgtccatg
     1201 cccgggcgta gcggtatgga gctgatcaag ctggtgcatg ctgaaagacc caaactgcgc
     1261 attctcgtgc tcagcatgca tcaggagttg cagtacgccg tgcgcgccat caaggccggt
     1321 gccggcggtt acctgaccaa ggaaagcgcg ccggatcaac tggtccaggc cctgcgcagg
     1381 attgccgcgg gtggcgccta catcagtgcc gaggtggccg aacaactggc cttgggcgcc
     1441 atgccgggcg gggtttccgc cgcgcacgag acgttgtcgg accgcgagtt cgaggtgttt
     1501 agcctgattg tggccggcaa ttccgtgacc aatattgcgg cgcgcctcaa tctttcggtc
     1561 aagaccatca gcacccacaa gaccaacttg atgcacaaga tgggcctgca aaaccagagc
     1621 gagttgattc gttatgcgat taaaaacgaa gtgggtgacg ccctggatca ttgaatacgg
     1681 cggtggcggc tgattgacgt ttatcaacac gattggcgct ggcggtacat aagcttgcca
     1741 gatcgaaagt ctgttgatgg agtcggtcat gaaagcttcc ctggtgcaaa tgctggttca
     1801 tcttgacgct tcgccgcagg tggcgcaacg actgacggcg gctcgccaga tcgcgcaggc
     1861 gcatggtgct gctgtgacgg cgttgtacgc ggtgacgccg aactttgtag aacttccttt
     1921 cgtgcccgaa ctcggccaaa acgtcgcggc cgatttgcgc gagatcgacg acgagttgcg
     1981 ggcaagcgcg cgcgccactt acgagcaatc gctgaccgat tccagtgtct acatggcatg
     2041 ggccgagatt cgcgacgatc cggtaatttc cttgtttgca cgacaggctt tctttgccga
     2101 tctgctggtg ctgggtcagc acgacccttc cggcaagcca tcgagcgggg tgccggctga
     2161 ttttgccgaa agcgttctgg tcgccagcgg caaaccggcc ttgatcctgc cctatgcggg
     2221 catgctgcca gcggtgatcg gcgaaaccgc tgtgatcgcc tggaagccta cccgcgaggc
     2281 tgcactagcc gtcagcgcag ccatgccgct gctacaacgc gcgcggcgcg ttcatgttct
     2341 tgcctggcgt ggtgaggaga tggcggtcga cagcaagctg ttgagcctgg acggctacct
     2401 caagcttcat ggggtggatg cggtttggca cagccagggc gacgagcccc cggcactggg
     2461 tgagttactg ctgtcgcgcg catgcgatct ggatgccgac ctgctggtga tgggctgcta
     2521 tggccatagc cgtgcgcgcg agtggattct gggcggcacc tcgcgcacgg tgctccgctc
     2581 catgaccttg ccggtgttga tggcgcactg atggcacttt gatggtgggt gcaacttgcg
     2641 caacgatggc gtcacttttc tctgtatttc acgcttgaca agcttcaatg gcgccagagg
     2701 aatcgggcgc tattgtggcg aacaattcca cccaggccga agccctccat ctcgaaggac
     2761 acgatgatga aacccgattg gatgctgatt gccaatgctt ctgaggcgcg gctgctgcgg
     2821 cgcgaacagg gcaatctgct ggtggttctc gaaaacttcg agcatccgga gagccgcagc
     2881 aaggtcagcg accaggtgga tgacaagatg ggatgggaga agtctgaccg gggctttggc
     2941 ggctcggcct ac
//
LOCUS       DASUIV010000175         3829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1342935, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000175 DASUIV010000000
VERSION     DASUIV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342935"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFW59_06460"
     CDS             <1..261
                     /locus_tag="VFW59_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S) protein"
                     /protein_id="HEX5363891.1"
                     /translation="ARRLPPFALRLLARDEDVEVRRIVAARMLAADAATMLADADWVV
                     RLEAAQHAPLESIAELVDDIEPDVRSVVRQRLGEFLQGDHEN"
     gene            292..1257
                     /locus_tag="VFW59_06465"
     CDS             292..1257
                     /locus_tag="VFW59_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363892.1"
                     /translation="MSIYTPDETVLRKLAADIEAIPPRPHHSRLLELANSIRPGCTFS
                     HALDRGGWYRPGGVIAADGRVIADNLEIWARAELGDCGGDMHELVERHADSGLMVTRH
                     SGRTHYFVAAYGPMPSDFLQLEVEELQEVLDRNLIDAANPPDDLQELTEPLAPEVLDA
                     QAVGAPRYHFRRLIDVRQMVGKASSAAGRGKGLSRLLPEWSHSSAAARGHFSDHWIVG
                     LREHQDRYRNPVVSVSLVSRHARELKPFHWNIELSGVEMLAQLQAFDRAAGYPSAWYF
                     HLVAGTFTPPKVAYAVASDLDAGFGYLPDTEVALLRNWVDAPYSV"
     gene            1543..1797
                     /locus_tag="VFW59_06470"
     CDS             1543..1797
                     /locus_tag="VFW59_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5363893.1"
                     /translation="MTQDREVDLTAGMCVDDVASLGRLVRQVRNRQGMTQADILGLAS
                     TGNRFIVELENGKPTVRLDKVLAVLDVLGLELQVRKKGRR"
     gene            1797..3041
                     /locus_tag="VFW59_06475"
     CDS             1797..3041
                     /locus_tag="VFW59_06475"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX5363894.1"
                     /translation="MDALDVFRGGEKVGTLYDEMPLRFEYAESWCNRPDAIPVDPGLD
                     TAVASHAGAAVEAYFENLLPEARVREFLQLRYQTTTVFGLLKETGGDTAGDLVLVPRG
                     GHPGAPSYRAASWEEIGASIGTPSGGLQEQSEEGIRISLAGAQTKTSVLLHADGTPAI
                     PLGTSPSTHIVKPDIKGMEGVWCSALNETMVMKLAAAVGMGVAETAYQPLIKACVVKR
                     YDRVPKADGNIVRLHQLDLCQLDGKPSTIKYEADGGPGLARCYRILRENGVPASDLKR
                     LLQWVFFNLFVGNHDGHAKNLSLYYPPGEGARLAPFYDLLSTTLYAGLSRKFALRLGG
                     ENLPGKMDRACIERMCAELGVKSGYAVKLADEVAAGIAAHTDGVAHELAKITEPGSEQ
                     TMLERLSQHILGNAKKVSVRLR"
     gene            3275..>3829
                     /locus_tag="VFW59_06480"
     CDS             3275..>3829
                     /locus_tag="VFW59_06480"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX5363895.1"
                     /translation="MRNTALLTDQQLEAEIARLRTSIEMWAKARDLWFDCGFASYIEH
                     VDGEPTDLPIVTLLLCEGELRDVLFGEDSRCYETEFSKLLNDLGYWYENRDGVSAAIY
                     PVDELLAEKFSEYFHWQWVCSLIKEDTADVYEELYAHFANRPDDLYRLNWREFEILLF
                     RIFQSHGFKAELGPGRGDEGIDLRL"
BASE COUNT          781 a          993 c         1228 g          827 t
ORIGIN      
        1 gcacggcgtc ttcctccttt cgcattgcgt ctgctggcgc gggatgagga tgtggaggta
       61 cgccgcatcg ttgccgcgcg catgctagct gccgacgcgg caacgatgct ggccgacgcg
      121 gattgggtcg tgcgcctgga agccgctcag catgcgccgc tggagtccat cgcggaactg
      181 gtggatgaca tcgagcccga tgtgcgcagt gtggtgaggc agcgtctggg cgaattcctg
      241 caaggggatc atgaaaacta acagtatcgg gaaggcaaag aggataagac catgagcatt
      301 tacacaccag atgaaacagt attgcgcaaa ctcgcagccg acatcgaggc gattccgccg
      361 cgcccccatc acagccggct gctggagttg gccaacagta ttcgtcccgg ttgcaccttt
      421 tcgcatgcgc tggatcgcgg cggctggtac cgtcccggcg gcgtcattgc ggcagacggc
      481 agggtcattg ccgataatct ggaaatatgg gcgagggcgg agctgggaga ttgcggcggc
      541 gacatgcacg agctggtcga gcgtcatgcc gacagcggtc tgatggtgac gcgccacagc
      601 ggacgcaccc attacttcgt cgcggcctac ggcccgatgc ccagtgattt tctgcaactc
      661 gaggtggaag agctgcagga ggtgctggac cggaatctga tcgatgcagc caacccgccg
      721 gatgacttgc aggaactgac cgaaccgctg gctcccgagg tgctcgatgc ccaggcggtc
      781 ggtgcgccac gttaccattt tcgccgcctg atcgatgtgc gccagatggt cggcaaggcg
      841 tcctcggccg cagggcgcgg caaggggctg tcgcgcctgt tgccggaatg gagtcatagc
      901 agcgcggcgg cgcgcggtca tttcagcgac cactggatcg tcgggttgcg cgagcatcag
      961 gatcgttacc gcaatccggt cgtgtccgtc tcgctggtgt cgcgtcatgc gcgtgaactc
     1021 aagccgttcc actggaacat cgagctttcc ggcgtggaga tgctggcgca gctgcaggcc
     1081 ttcgatcgcg ccgccggcta tccttccgcc tggtatttcc atctggtggc gggcaccttc
     1141 acgccgccca aggtcgccta tgcggtggcg agcgatctgg atgccggatt cggctacctg
     1201 ccggataccg aagtcgccct gctcagaaac tgggtggacg caccgtactc cgtctagcct
     1261 aactgtccgt actgccgata tattctcgaa aaatatatcg gccgttttcc ttcccggtgc
     1321 ggcgggccgc gagggctgaa gcacttgcga tgcggcctcc tttaatcatg taaatcaacc
     1381 ccgtgccggt cggtatggtt tcagtgccta taattaccta tcggtaatat atgtgccttc
     1441 ctagtggatg gttaagcata aatttaccga ccggtaattt tttgttgcga cttggcggag
     1501 aatcagccat agtattaccg aacggtaatg aaaggagggg ctgtgactca agatagggag
     1561 gtcgacctga cggcggggat gtgcgtcgac gatgttgctt cgctggggcg cttggtgcgg
     1621 caggtacgca accggcaggg gatgacgcag gcggacatcc ttggcttggc tagcacgggc
     1681 aatcgtttca tcgtggagct ggagaacggc aagccgaccg tgagactgga taaggtgctg
     1741 gccgtgctcg acgtgctggg actggagttg caggtcagga agaaaggcag gcgctgatgg
     1801 atgcgctgga tgtattccgc ggcggcgaga aagtcggcac cctgtacgat gaaatgccgt
     1861 tgcgtttcga atatgccgaa tcctggtgca acaggccgga tgccatcccc gtcgatcccg
     1921 gcctggatac tgccgtcgcc agtcatgcgg gggcggcggt cgaggcctat ttcgagaacc
     1981 tgttgccgga agcgcgggtg agggagttcc tgcaactcag gtaccagacc accacggtat
     2041 tcggtttgct gaaggagaca gggggcgaca cggcgggcga cttggtgctg gtgcctcggg
     2101 gcgggcatcc tggtgcgccg tcataccggg cagcaagctg ggaagagatc ggtgcatcaa
     2161 tcggcacacc ttcgggcggt ttacaagagc agtccgagga gggcatccgt atctctctgg
     2221 cgggagcgca gacgaaaacc agcgtgcttc ttcatgcgga tggtacgccc gcaatcccgc
     2281 tcggcacctc gccttccaca cacatcgtca agcccgacat caagggcatg gaaggcgtgt
     2341 ggtgttcggc gctgaatgag acgatggtga tgaagctggc ggcagccgtt ggaatgggcg
     2401 tggccgaaac ggcctaccag ccgcttatca aggcctgcgt ggtcaagcgt tacgaccgcg
     2461 tgccgaaagc ggatggcaac atcgtcaggc tgcatcagct cgacctgtgc caactcgatg
     2521 gcaaaccctc gacgataaaa tacgaagccg atggcggccc cggcctggcg aggtgttatc
     2581 gcatcctgcg cgaaaacggc gtgccagcat ccgacctcaa gcgcttgctg cagtgggtgt
     2641 ttttcaacct gtttgtcggc aatcacgacg gccatgccaa gaacttgtcg ctgtattacc
     2701 cgccgggtga gggagcccgg ctcgcgccgt tctacgactt gctttcgact acgctctatg
     2761 ccggactctc ccgaaagttc gcgctcaggc tgggcggtga aaatctcccg gggaaaatgg
     2821 atcgtgcctg tatcgaaagg atgtgtgcgg aactgggcgt aaagtctgga tatgcagtga
     2881 agcttgccga cgaggtagcg gcaggcattg cggcccatac ggacggcgtc gcacatgaac
     2941 tggcgaaaat taccgagccg ggcagcgagc agaccatgct ggaacgcctg agccagcaca
     3001 ttctcggtaa tgcgaaaaaa gtaagcgtaa ggttgagata gatgctccgg tgcgcgtgtc
     3061 gttctcgcgg ggcaaggtcg cggggacggg tgtgttcaag caggtgtcga agctggtcaa
     3121 gtcaggggag cgcagggctt ctgaaaatat acaggccttt tttctgcttg gtttcgcgat
     3181 gatgtagggc taggatgcgg gacgatgttt ggtggtcggt ctaggtaagt cgatgctgac
     3241 ccctttaatc cacctacggt gctaaggtga cgcgatgcga aatacggccc ttttgaccga
     3301 ccagcaactt gaagcggaaa tagcacgcct tcgaacctct attgagatgt gggcaaaagc
     3361 tcgcgacttg tggttcgatt gtgggtttgc ttcatacata gaacatgtag atggtgagcc
     3421 aactgatctt cccattgtga cattgctctt gtgcgaagga gagttgcgcg atgtcctgtt
     3481 tggtgaagat tcgcgatgtt atgaaactga gttctcgaag ctacttaatg acttaggtta
     3541 ctggtacgag aacagagacg gtgtatctgc tgctatttat ccggtcgacg aattacttgc
     3601 tgaaaaattc tcggaatatt ttcattggca atgggtgtgt agccttataa aggaagacac
     3661 tgccgatgtt tatgaagagt tgtatgctca tttcgcaaat cgaccggatg atctctatcg
     3721 tttgaactgg cgtgaatttg aaattctgct attccggata tttcaaagtc atggtttcaa
     3781 agcagagctt ggtccgggca gaggcgatga aggaattgat ttgcgactt
//
LOCUS       DASUIV010000176         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_693833, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000176 DASUIV010000000
VERSION     DASUIV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693833"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /gene="argA"
                     /locus_tag="VFW59_06485"
     CDS             <1..673
                     /gene="argA"
                     /locus_tag="VFW59_06485"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX5363896.1"
                     /translation="SKGELLKELTVAQAQKVLGGKRKLSDDVSLFLPCAIRACEAGVA
                     RTHLISRHTDGSVLQELFSDEGIGSMVVESALNTLREASIDDIGGIIELLRPLEESGI
                     LVRRSRELLEREIDRFVVLEHDHRIIGCAALYPFPDEAAAELACLAVDAQCRDRGYGE
                     AVLNHMISVAKSQGLKKLFVLTTRTAHWFIERGFKESDVSELPEQKKSLYNYQRKSKV
                     FVRKI"
     gene            complement(703..1248)
                     /gene="rfbC"
                     /locus_tag="VFW59_06490"
     CDS             complement(703..1248)
                     /gene="rfbC"
                     /locus_tag="VFW59_06490"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX5363897.1"
                     /translation="MKAIQTAIPDLLILEPRVFGDERGFFFESFNRRTFAELTGRDVE
                     FVQDNHSRSVRNVLRGLHYQIQHPQGKLVRAVQGSVLDIVVDIRRSSPTFGKHVSAEL
                     SAENKRMMWIPEGFAHGFVVLTETAEFLYKTTDYWYPEHERCIRWDDPVLAIDWQLQG
                     APSLSAKDSLGKAFAEAETFA"
     gene            complement(1245..2135)
                     /gene="rfbA"
                     /locus_tag="VFW59_06495"
     CDS             complement(1245..2135)
                     /gene="rfbA"
                     /locus_tag="VFW59_06495"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX5363898.1"
                     /translation="MTKRKGIILAGGSGTRLHPVTLAVSKQLLPIYDKPMIYYPLSTL
                     MLAGINDILIISTPQDTPRFQQLLGDGSAWGLNLQYAVQASPDGLAQAFVIGREFIGN
                     DASALVLGDNIFYGHSFHQQLESATQQKDGATVFAYHVRDPERYGVVEFDAGGRAISL
                     EEKPTQPKSNYAVTGLYFYDNSVLDIAANLKPSPRGELEITDVNRTYLEQGRLSVEIM
                     GRGYAWLDTGTHESMLDASQFVQTIEQRQGLKVACPEEIAYRKGFIDAAQLERLAAPL
                     LKSGYGNYLMQVLREGTFSL"
     gene            complement(2132..>2440)
                     /locus_tag="VFW59_06500"
     CDS             complement(2132..>2440)
                     /locus_tag="VFW59_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX5363899.1"
                     /translation="AHAIRTAQRQPEVSGLYHLVAGGETSWHGYATYVIEFARQAGIP
                     LRVDAIQPVPTSGFPTPAKRPHNSRLDTTKFQDTFGLFLPPWQNGVARMLAEILDKQP
                     "
BASE COUNT          458 a          751 c          760 g          471 t
ORIGIN      
        1 ttccaagggc gagctgctca aggagctcac cgtcgcccag gcgcagaaag tgctgggagg
       61 gaaacgcaag ctgtcggacg atgtgagcct gttcctgccc tgcgcgatcc gcgcctgcga
      121 agccggggtg gcgcgcactc atctgatcag tcgccatacg gacggctcgg tactgcagga
      181 gctgttcagc gacgagggca tcggcagcat ggtggtggag agcgcgctca acaccctgcg
      241 cgaggcttcc atcgacgaca tcggcggtat catcgagctg ctgcgcccgc tggaggagag
      301 cggtatcctg gtacggcgtt cacgagagtt gctggaacgc gagatcgacc gctttgtggt
      361 gctggagcac gaccaccgca tcatcggctg cgccgcgctt taccccttcc cggacgaggc
      421 cgccgccgaa ctcgcctgtc tggcggtgga tgcgcagtgc cgcgaccgcg gctacggcga
      481 ggccgtgctc aaccacatga tctcggtcgc caaatcgcag gggctgaaga aactgttcgt
      541 gctgacgacg cgcaccgcac actggttcat cgagcgcggc ttcaaggaga gcgacgtctc
      601 cgaactgccc gaacagaaga aatccctata caactatcag cgcaagtcca aggtgttcgt
      661 gcgcaaaatc taggcacggg aaaagcgcac tggctgcgag cgctatgcga aggtctccgc
      721 ctcggcaaac gccttgccca gactatcctt ggccgacagc gagggggcgc cctgcagctg
      781 ccagtctatc gccagcaccg gatcatccca gcgtatacag cgctcgtgtt ccgggtacca
      841 gtagtcggtg gtcttgtaca ggaactccgc cgtctcggtc agcaccacga agccatgcgc
      901 gaaaccttcc ggtatccaca tcatgcgttt gttctcggcg gacagttctg cgctgacatg
      961 cttgccgaaa gtcggcgaac tgcgacggat atccaccact atgtccagca cactgccctg
     1021 caccgcgcgc accagctttc cctgcgggtg ctggatctgg tagtgcaggc cgcgcagcac
     1081 gtttctcacc gagcgcgaat ggttgtcctg cacgaattcg acatcgcgcc ccgtcagttc
     1141 ggcaaaggtg cggcggttga agctctcgaa gaagaacccg cgctcatcgc cgaacaccct
     1201 gggttcgagg atcaacagat ccgggatggc ggtctgaatc gctttcatag cgaaaacgtc
     1261 ccttccctga gcacctgcat cagatagttg ccatagccgc tcttgagcag cggcgctgcg
     1321 aggcgttcca gttgcgcggc atcgatgaat cccttgcggt aggcgatctc ttccgggcag
     1381 gcgaccttga gcccctggcg ctgctctatg gtctgcacga actggctcgc atccagcatg
     1441 gactcgtgcg tgccggtatc cagccaggca taaccgcgcc ccatgatctc gaccgacagc
     1501 ctgccctgct ccagataggt gcggttcacg tcggtgatct ccagttcgcc gcgcggcgag
     1561 ggcttgaggt tggcggcgat gtccagcaca gagttgtcgt agaaatacag cccagtcacc
     1621 gcatagttgg acttgggctg cgtcggcttt tcttcgagcg agatcgcacg gccgcctgcg
     1681 tcaaactcca ccacgccata acgctcggga tcgcgcacgt ggtaggcgaa caccgtcgcg
     1741 ccatccttct gctgggtcgc actttcgagc tgctggtgaa acgagtgtcc gtagaagatg
     1801 ttgtcgccca gcaccagtgc ggaggcgtcg ttgccgatga actcgcgtcc gatcacgaag
     1861 gcctgcgcga gtccgtcagg cgatgcctgt acggcatatt gcagattcaa gccccaggcg
     1921 ctgccgtcgc ccagcaattg ctggaagcgc ggcgtgtcct gcggcgtgga gatgatcagg
     1981 atgtcgttga tacccgcgag catcagcgtg gacagcggat agtagatcat cggcttgtcg
     2041 tagatcggca gcagctgctt ggaaaccgcc agcgtgaccg gatgcagacg ggtgccggag
     2101 ccgcctgcca gaatgatgcc cttgcgtttt gtcatggttg tttgtccagt atttctgcca
     2161 gcatgcgtgc cacaccattt tgccacggtg gcaggaacaa gccgaaagtg tcttgaaatt
     2221 tcgtcgtatc cagccgcgaa ttatgcgggc gctttgcggg ggtggggaag ccgctggtcg
     2281 gcacgggctg gatggcatcg accctgagcg gaatacccgc ctgccgcgcg aactcgatca
     2341 cgtaagtggc gtaaccgtgc caggaggtct cgccgcccgc taccagatga tacagtccgg
     2401 atacttccgg ctgcctttgc gcggtgcgga tcgcatgggc
//
LOCUS       DASUIV010000177         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1159047, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000177 DASUIV010000000
VERSION     DASUIV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159047"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFW59_06505"
     CDS             <1..444
                     /locus_tag="VFW59_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5363900.1"
                     /translation="AADQAAAVCVAQGIEHGLIELGGDIQVIGPHPDLSPWIVGIRHP
                     RLADGIMATVEISRGAIASSGDYERYIVVDGVRYCHLLDPSTGWPVQGLASVSVISEQ
                     CLVAGSVATMAMLKGNAGKQWLAALGVPHLWMDEAGNSGGNIHPQ"
     gene            complement(504..944)
                     /locus_tag="VFW59_06510"
     CDS             complement(504..944)
                     /locus_tag="VFW59_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX5363901.1"
                     /translation="MSTISGISGMGAMPQAMSGASGRMAPQTKMSNLFQQIDTANTGR
                     ITKAQFEQAFNNMNPPASFKAIGLDAAFSKLDPAGRGSVSKDDFINGMKSMMTHRRHH
                     NEANVEKTSAPVPQTVPSSTAALNALGGAPDVSNVIGGNISTSA"
     gene            complement(1077..1622)
                     /locus_tag="VFW59_06515"
     CDS             complement(1077..1622)
                     /locus_tag="VFW59_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEX5363902.1"
                     /translation="MTHETEIIAATQLWLEKAVIGLNLCPFAKAVHVKKQIRYVVSAA
                     TTPEALLEDLMTELRRLQDTSPEEIDTTLLIHPYVLTDFLDFNDFLDTVDIAVAEPEF
                     GDEFQVASLHPQYQFAGTAVDDIENYTNRSPYPTLHLLREDSVDRAVEAFPDADQIPD
                     TNIETLKRLGHEGWAKLGLPK"
     gene            complement(1693..2673)
                     /locus_tag="VFW59_06520"
     CDS             complement(1693..2673)
                     /locus_tag="VFW59_06520"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX5363903.1"
                     /translation="MHTPHPFSSLTPDAVLDALEGIGLHSDGRLLALNSYENRVYQVG
                     MEDGPPLIVKFYRPERWSALAILEEHAFVSELAEREIPVVPALKIADRTLHDFNGYRF
                     AVFTRHGGRAPELDDRDTLEWLGRFIGRIHAVGKLERFAHRPTLDIESFGVEPVEYLL
                     AHDFIPAELIEVYRGVTAQALDGVRRCFGRAGTVPHLRLHGDCHVGNVLWTDAGPHFV
                     DFDDSRMGPAIQDLWMLLSGERDEQTRQLGDLLAGYEDFCEFDRRELHLLEALRTLRL
                     IHYSAWIARRWNDTAFTTAFPWFNTQRYWQDRILELREQIAMMDEPPLHC"
     gene            complement(2733..>3500)
                     /locus_tag="VFW59_06525"
     CDS             complement(2733..>3500)
                     /locus_tag="VFW59_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5363904.1"
                     /translation="NQPAVKNFDPQDLWSHSMTIAIVMDAISQAMPKTLRPDENQIFL
                     AGLLHDIGLMALHHIDREASDELHHQLRLQPKRSMQDIEQELLGMTHGFIGAQLVRHW
                     FLPKEIIEVVGLHHSAHIGALTRTNPLVRLVNLAEKLLPDFGIAEHTSAAIDESEWHE
                     LGIAPARADELSALVNELAMQVVQLPETHDATQASMPLKDSSPLGVEQYVAQRMASAQ
                     PEPVPADVESGFSAALVRLKKPVQWIGAKFRRLRRRD"
BASE COUNT          694 a         1056 c         1053 g          697 t
ORIGIN      
        1 gccgccgatc aggccgcagc cgtctgtgtt gcgcagggca tcgaacatgg tctgatcgaa
       61 ctgggcggcg acatccaggt gatcggcccg catcccgacc tctcgccctg gatcgtcggc
      121 atccgccatc ccagactggc ggatggcatc atggccaccg tcgagatctc gcggggtgcc
      181 atcgcctcca gcggcgacta tgagcgttac atcgtggtgg acggagtacg ttactgccac
      241 ctcctcgacc cctctaccgg ctggccggtg cagggcctgg cttcggtgag cgtcatcagc
      301 gaacaatgtc tggtcgcggg cagtgtcgcg acgatggcaa tgctaaaagg caacgctggc
      361 aagcagtggc tcgcagcgct tggcgtgccc catctctgga tggatgaagc cggaaattca
      421 ggcggaaata ttcatcccca atgagaaacg ccgccacgaa ctcgtggcgg cgcttcccgg
      481 acatcttgat tcagaggtgt cgatcaggca gaggtgctga tgttgccacc gatcacgttg
      541 ctgacgtctg gcgcaccgcc caacgcattc agtgcagccg tgctgctcgg cacggtctga
      601 ggaaccggcg cgctggtctt ctcaacattc gcttcattgt gatggcgacg atgcgtcatc
      661 atggacttca tgccgttgat gaaatcatcc ttggagacgc tgccacggcc ggcaggatcg
      721 agcttgctga aagcggcatc cagaccgatg gccttgaaag aagcgggcgg gttcatgttg
      781 ttgaacgcct gttcgaactg ggccttggtg atgcggccgg tattggcagt atcgatctgc
      841 tggaacaggt tggacatctt ggtctgaggc gccatgcggc cgcttgcacc cgacatcgcc
      901 tgcggcattg cgcccatgcc tgaaatcccg ctgatagtgg acattgcttc tcctgaaatt
      961 aataaagatg gataaattat tcggccgcca tcgtagcgca gaatgcgcat cacactgggc
     1021 cgaacagggg gaataaatcc cctctaatca aaccgggacg acatccggac gagcgcctat
     1081 ttcggcaatc ccagcttcgc ccagccctcg tgccccagcc gcttcagcgt ctcgatattc
     1141 gtgtcgggga tctggtcggc atcggggaag gcttccaccg cgcggtccac gctgtcttcg
     1201 cgcagcaggt gcaaggtcgg gtaaggcgaa cgattggtgt aattctcgat atcgtccacc
     1261 gctgttccgg cgaactgata ttgcggatgc aggctggcaa cctggaactc atcgccgaat
     1321 tccggctccg caacggcgat atcgaccgta tccagaaaat cgttgaagtc gaggaagtcg
     1381 gtgagcacgt aggggtggat caacagggtg gtatcgatct cttccgggga cgtgtcctgc
     1441 agcctgcgca gctcggtcat cagatcttcc agcaaggcct ccggcgtggt ggccgcgctc
     1501 accacataac gtatctgttt cttgacatgc accgccttgg cgaaggggca cagattgagt
     1561 ccgatgacgg ctttttccag ccacaactgg gtggcggcga tgatttcggt ttcatgggtc
     1621 atgacgctct ttcttggttc gggtgttaaa aacagatcgg atgaggatga agtcgtcatc
     1681 cgtttgcgca gttcaacaat gcagcggcgg ctcgtccatc atcgcgatct gttcgcgcaa
     1741 ttccaggatg cggtcttgcc agtagcgctg ggtgttgaac caggggaaag cggtggtgaa
     1801 tgcagtgtcg ttccagcgcc gcgcgatcca tgccgagtag tggatcagac gcagggtgcg
     1861 caaggcttcg agcagatgca gctcgcgccg gtcgaattcg cagaagtcct catagcccgc
     1921 caacaggtcg cctaactggc gtgtctgttc atcgcgctcg cccgacagca gcatccacag
     1981 gtcctgtatc gcagggccca tgcggctgtc gtcgaaatcc acgaagtgcg gccccgcgtc
     2041 ggtccacagc acattgccga catggcagtc gccgtgcaga cgcaaatgcg gcaccgtacc
     2101 ggcgcgaccg aagcagcgcc gcaccccatc cagcgcctgc gcggtcacgc cgcgatacac
     2161 ctcgatcaat tctgccggga tgaaatcgtg cgccagcaaa tactcgacag gctccacgcc
     2221 gaagctctcg atatccagcg tcgggcgatg ggcgaaccgc tccagcttgc cgaccgcatg
     2281 gatgcgtccg atgaagcgtc ccagccattc cagcgtgtcg cggtcgtcca gttcgggcgc
     2341 ccgcccgccg tgccgggtga acacggcaaa acgatagccg ttgaaatcgt gcagcgtgcg
     2401 gtcggcaatc ttcaacgcgg gcaccaccgg aatctcgcgt tcggccagct ccgagacgaa
     2461 cgcgtgctcc tccagaatcg ccaaagccga ccagcgttcg ggacggtaga acttgacgat
     2521 cagcggtggc ccgtcctcca tgcccacctg atagacgcga ttttcataac tattgagcgc
     2581 gagcaaccgg ccatcgctgt gcaggccgat gccctccagc gcatcgagca cggcatcggg
     2641 ggtgagagaa ctgaaaggat ggggagtatg catgggcggg attgtacggc actacgacgg
     2701 cgaccgtcgc ccccgcgtca tggaattgac ggctagtccc tgcggcgcaa tctgcggaac
     2761 ttcgcgccta tccattgcac cggtttcttc aaccgcacca gcgccgcaga gaaacccgat
     2821 tcgacatcgg ccggaaccgg ctcgggctgg gcggatgcca tacgctgtgc cacatattgc
     2881 tcgacgccca gcgggctgga gtccttgagt ggcatggagg cctgggtggc atcatgggtc
     2941 tcgggcagtt gcacgacctg catcgccagt tcgttgacca atgcggagag ctcgtctgcg
     3001 cgtgccggag cgatccccaa ttcatgccac tcgctctcgt cgatggcggc gctggtatgt
     3061 tcggcgatgc cgaaatccgg caacaacttt tcggctagat tgaccagcct gaccagcgga
     3121 ttggtgcggg tcaatgcgcc gatgtgcgcc gagtgatgca agcccaccac ctcgatgatc
     3181 tccttgggaa gaaaccagtg ccgcaccaac tgcgcaccga tgaaaccatg ggtcatgccc
     3241 agcaattcct gttcgatatc ctgcatggaa cgcttgggct gcaaacgcag ctgatgatgc
     3301 aactcgtcgc tcgcctcccg atcgatatga tgcaaggcca tcaagccgat gtcatgcagc
     3361 aggcccgcga gaaatatctg gttctcgtcc gggcgcagcg tctttggcat cgcctgcgaa
     3421 atcgcatcca tcacaatggc gatggtcatg ctatgcgacc acaagtcttg cggatcgaaa
     3481 ttcttcaccg caggctgatt
//
LOCUS       DASUIV010000178         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_42284, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000178 DASUIV010000000
VERSION     DASUIV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42284"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..559
                     /locus_tag="VFW59_06530"
     CDS             125..559
                     /locus_tag="VFW59_06530"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HEX5363905.1"
                     /translation="MAKTTELEAAYRATTYRVFLPGGVCDLRPGVASETLRCWLETAG
                     AGRFAILTAHNPGSSLIDAGQNALRQSQLECDLLESGYEPYAGENVADQDAWIDEESC
                     FIPDISVAEALALGRKYGQNAIICGGDDGVPELVWIGDQATP"
     gene            complement(594..1412)
                     /locus_tag="VFW59_06535"
     CDS             complement(594..1412)
                     /locus_tag="VFW59_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5363906.1"
                     /translation="MMRSVPAFTPFFRLLTALLIATVVAGCGLFPETKDETIGWSANK
                     LYAEAKEAQSDGAWDKAAKYYEKLEARYPYGRFAQQAQLELGYVYWKAGETGSALAAT
                     DRFIKLHPTHPAVDYAYYLKGLINFNEDLGWAGYISTQDPTERDPKALRESFDAFREL
                     ATRFPNSKYTPDAIQRMNYLVNAMASHEVHVARYYYNRGAYVAAANRAQNAIKTYPSA
                     PAIEEAMYILIAAYDKLGMTDLRDDADRVMRKNFPDSRFYAEGLERKSRWWHLW"
     gene            1411..2370
                     /gene="rluD"
                     /locus_tag="VFW59_06540"
     CDS             1411..2370
                     /gene="rluD"
                     /locus_tag="VFW59_06540"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEX5363907.1"
                     /translation="MKNPLEDRADYSRTQANPACVPDRCAGRRLDQVLAELWPQHSRN
                     RLQGWVRDGLVEVDGRIESEPKRKLLGGERLTLNEPREARELAEQPEDIPLDIVFEDE
                     ALLVINKPAGLVVHPGSGNWSGTLMNALLHHVPGIAEVPRAGIVHRLDKDTSGLLVVA
                     KTLEVQTDLVRQLQARTVKRQYLAFVAGVVYVQSTIDAPIGRHPAQRVKMAVVPETRG
                     GKAAVTHYRGLEQFDHCALVECTLETGRTHQIRVHMASVKHPLIGDKVYGRVDPRLPE
                     FPRQALHATRLALIHPLEQRLRQWEVPMPADMAELLETLRDDG"
     gene            2363..3106
                     /gene="pgeF"
                     /locus_tag="VFW59_06545"
     CDS             2363..3106
                     /gene="pgeF"
                     /locus_tag="VFW59_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX5363908.1"
                     /translation="MAECYVPDWAVPANIRALQTTRSGGTSKAPWASFNLGNHVGDGA
                     AAVAANRALLVSRLPAEPLWLTQVHGIVAVDADQRPKIREADAAFARQPGSVCVVMTA
                     DCLPVLLCDRQGTIVAAAHAGWRGLAAGVLESTVAAMKVDPGELLVWLGPAIGPRCFE
                     VGNEVRDAFIADNSAAGLAFEPHEAGKWLCDIYLLARQRLQRMGIMAISGGGECTVTE
                     ADRYFSYRRDGVTGRMASLIWLESLVQSV"
     gene            3136..>3516
                     /locus_tag="VFW59_06550"
     CDS             3136..>3516
                     /locus_tag="VFW59_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5363909.1"
                     /translation="MSTLSWIIVVALAGGLISVLAAAAMTFAVGTHRVNMLISYAIGA
                     LLGAAFLEILPHALEHGQPHRMAATVLFGILVFFVLEKLVLWRHCHHDHCEAHDAQAP
                     AHDHGRSGLLILVGDTFHNFVDGIL"
BASE COUNT          625 a         1008 c         1207 g          676 t
ORIGIN      
        1 gacgaagcga gcaacagcat cagcaaggcc gagacgcgga cgcgccagtt gtcgcgcaag
       61 ctgcgtgatg tcgaggcgct gccggcgagc gagtcggaac aactgatcgg ggtcgcggaa
      121 ttcgatggcg aagacgactg aactggaagc cgcctaccgg gcgacgacct accgtgtatt
      181 cctgccgggc ggtgtttgcg acctgcgccc cggcgtcgct tcggagacat tgcgttgctg
      241 gttggagacg gctggtgccg gcaggtttgc gatcttgaca gcgcacaacc cgggttcgtc
      301 gctgatcgac gccgggcaga atgcgctgcg ccagtcgcaa ctggagtgtg atctgctgga
      361 atcggggtac gagccctatg ccggcgagaa tgtggccgac caagacgcct ggatagacga
      421 ggagagctgt ttcatccccg atatttcagt cgccgaggcg ttggcgctgg gcaggaaata
      481 cgggcagaac gcgatcatct gcggcggcga tgacggggtg cccgaactgg tctggatagg
      541 cgaccaggca accccatagc cataacgggt tgcctgacgc aattaccaaa gatttaccag
      601 agatgccacc agcgcgattt gcgctcgagg ccttcggcgt agaagcggct atccgggaag
      661 ttcttgcgca tcacacggtc ggcgtcatcg cgcagatcgg tcatgcctag cttgtcgtag
      721 gcggcgatca ggatatacat cgcttcctcg atggcgggtg ccgaagggta ggtcttgatg
      781 gcgttctggg cgcggttggc ggccgcgacg taggcgccgc ggttgtagta gtagcgggcg
      841 acatgaacct catgcgaggc catggcattg accagatagt tcatgcgctg gatggcgtcc
      901 ggcgtgtatt tgctgttggg aaagcgtgtt gccagttcgc ggaaggcgtc gaacgattcg
      961 cggagggcct tggggtcgcg ttcggtcggg tcctgcgtgc tgatgtagcc ggcccagccg
     1021 agatcctcgt tgaagttgat cagccctttg aggtagtagg cgtagtcgac cgccggatgg
     1081 gtcgggtgca gcttgatgaa gcggtcggtg gcggcgagtg ccgagccggt ttcgccggcc
     1141 ttccagtaaa cataaccgag ttcgagctgg gcttgctggg caaagcgccc gtacgggtaa
     1201 cgcgcttcca gcttttcgta atacttggcg gccttgtccc aggcgccgtc ggattgcgct
     1261 tccttggctt cggcatacag cttgttggcc gaccagccga tggtttcgtc cttggtttcg
     1321 ggaaaaagac cgcagccggc aacaacggtt gcgatcaata gcgccgtcag caggcgaaag
     1381 aagggggtga atgcgggtac acttcgcatc atgaaaaatc ctttggaaga ccgcgcggat
     1441 tatagccgaa ctcaggcgaa tcccgcctgc gttcctgatc gctgcgccgg gcggcgtctc
     1501 gatcaggtgc tcgccgaact atggccgcag cattcgcgca atcgcctgca ggggtgggtg
     1561 cgggacggac tggtcgaggt tgacggacgc attgagagcg aaccgaagcg caaactgctg
     1621 ggtggcgaga ggctgaccct gaacgaaccc cgcgaggcca gggaactggc cgaacagccg
     1681 gaagacattc cgctcgacat cgtcttcgag gacgaggcgc tgctggtgat caacaagccg
     1741 gccggcctcg ttgtgcaccc gggcagcggc aactggagcg gcacgctgat gaatgccttg
     1801 ctacatcacg tgcccgggat cgccgaagtg ccgcgggcgg gcatcgtgca tcggctggac
     1861 aaggacacca gcggcctgct tgtcgtcgcc aagacgctgg aagtccagac cgacctggtg
     1921 cgccaactgc aggcgcgcac cgtcaagcgt cagtatctgg ctttcgtcgc gggtgtcgtc
     1981 tatgttcagt cgacgatcga tgcgccgatc ggccgtcacc cggcgcagcg cgtgaagatg
     2041 gccgtggttc cggaaacccg gggtggcaag gcggccgtca cccactaccg cgggctggag
     2101 caattcgacc attgcgcact ggtcgaatgc acgctggaaa ccgggcgcac gcatcagatt
     2161 cgcgtccaca tggcctccgt caagcacccg ctgatcggcg acaaggttta tggccgggtc
     2221 gatccgcgct tgccggaatt tccccggcaa gccctgcatg ccacgcgcct ggcgctgatt
     2281 catccgctgg aacagcgcct gcgccagtgg gaggtgccga tgccagccga catggctgag
     2341 ctgctggaaa ccttgcgcga cgatggctga gtgctatgtg ccggactggg ccgttccggc
     2401 gaacatccgg gccttgcaga cgacgcgcag tggcggaaca agcaaggcac cttgggcgag
     2461 tttcaatctc ggcaatcacg tgggtgatgg cgcggcagcg gttgctgcga accgcgcatt
     2521 gctggtttcc cgcctgccgg ccgaaccgtt atggctgacg caggtgcatg gcattgttgc
     2581 ggtcgacgcc gatcagaggc caaaaatcag ggaggccgac gcggcgttcg cgcggcaacc
     2641 gggcagcgtc tgcgtggtga tgacggccga ttgcctgccg gtgcttctgt gcgatcgcca
     2701 gggaacgata gtcgccgccg cgcatgccgg ctggcggggg ctggcggccg gcgtgctgga
     2761 aagtactgtt gccgcgatga aagttgatcc cggggaactg ctggtctggc tggggccggc
     2821 aatcggcccg cgctgctttg aagtgggcaa cgaggtgcga gatgccttca ttgcagacaa
     2881 ttcggcagct ggccttgcct ttgagccgca cgaggcgggc aaatggctgt gcgacattta
     2941 tcttctcgcg cggcaacgtc tgcagcgcat ggggatcatg gcgatcagcg gcggcggaga
     3001 gtgcacggtg accgaagccg accgttattt ttcctatcgc cgtgacgggg tgaccgggcg
     3061 gatggcgagc ctgatctggc tggagagctt ggtacagagc gtataatccg cgccttcccc
     3121 cgaatctggt ttcccgtgag taccctttct tggattattg tcgtcgcgtt ggccggcggc
     3181 ctaataagcg ttctggcggc ggccgcgatg acctttgctg tcggcacgca tcgcgtcaat
     3241 atgctgattt cctacgcgat tggcgcgctg ctgggagccg cgtttctgga aattctgccg
     3301 catgcgctgg aacacggaca gccgcaccgc atggcggcaa cggtcctgtt cggcattctg
     3361 gttttcttcg tgctggagaa actggtgctg tggcggcact gccaccacga ccactgcgaa
     3421 gcgcacgacg cgcaggcgcc ggcacacgat cacgggcgct ccggtctgct gatcctggtc
     3481 ggtgacacct tccacaattt tgtcgacggc atcctg
//
LOCUS       DASUIV010000179        13333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_415387, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000179 DASUIV010000000
VERSION     DASUIV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415387"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VFW59_06555"
     CDS             <1..617
                     /locus_tag="VFW59_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5363910.1"
                     /translation="RNVDEMIGEIRLVQESVNQIAGSVKEFVESTHAIAGMTQQVKDI
                     AEQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAEKSAQSASEIDQVTNSLNQKST
                     HVEGTVQAGLRALLTTQEHIERVATVLSAASAAVTQSSHGVNDIASSVAEQGLASNEI
                     SRNVERIAQMAEENHAAVQSNGQEIVRLEQLAGELRSAVSRFRV"
     gene            674..2746
                     /locus_tag="VFW59_06560"
     CDS             674..2746
                     /locus_tag="VFW59_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX5363911.1"
                     /translation="MQNKNAIVTKNEIPFPAGAVLVSKTDTKGVITYCNDAFEAISGF
                     SRAELIGRSHNIVRHPDMPPQAFKWLWDTLKSERPWRGMVKNRCKNGDFYWVRATVAP
                     IIEGGKIIGYVSVRRAPTRAQIAEAEKFYAELNQSGRQVVSKYEKLRIKNWSMKGKLQ
                     AAIQIPLLVILSVAQFYITGALKDDALRQAADRGDQIANQVIDSENMLMVTGQIGDVA
                     TRKLLLHKVTSSGNVKTAAVVRSPQLIEQYGGLPEEHISDDVQRQVIAGKKQSVMFAK
                     DAVGDPVMRIVTPYLASKDFHGTDCTSCHTVAEGEAVGASDVVIDLKPDFARIHSMEL
                     NVLLGQLGLQVFLFFFIGYCVDRFIRSPMADVGSEFRNIMEGNLDTELDISIHDEMGD
                     LLCEIQSMQAYLRTMVDEIVTPVTSMQRRIEDVDGRVSGVAANALTEQDHIQSIAATM
                     EELSQSITEVANMATDSLSDAKAMQRIVEENNGNMELSIQATTRVADTVQSSSRTISD
                     LGISIEKIGAIAKAIEEIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTA
                     TSTKDIARTIGEITSISGAAVKTMHDAVSEVETGIGLIRKNGEGLKEIMNASINVAGR
                     IEHIATAAGEQSQAGEGVANSLERINGLVDNNAQSASEAKQAMDELSKSASELRKAGY
                     PLTKCAME"
     gene            2900..3841
                     /locus_tag="VFW59_06565"
     CDS             2900..3841
                     /locus_tag="VFW59_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEX5363912.1"
                     /translation="MSDLLRNIDARTKLAGTNKLEILMFSLGKDMRNGREETFGINVF
                     KVREVMRIPAITRAPEMPPAVEGMVSLRGQLVPVIDLAKYIGMQTDGEPEIMIVTEYN
                     GHTQGFLVRAVDNILRLDWSLMRVPPAMLAAEMGGLVTAVTELKDKRLVMMLDVEKVL
                     AETGHFGDDEAVFNAVKPLGKERTIFFADDSSVARNQISRTLDAMQVKHLSAVNGRQA
                     WADLCRLADLADSTGVPLKETIQVILTDVEMPEMDGYMLTRKIKSDKRFNGIPVLMHS
                     SLSSTSNEQMGKAVGVDEYVPKFEAQKLSQTLARLLS"
     gene            3926..4873
                     /locus_tag="VFW59_06570"
     CDS             3926..4873
                     /locus_tag="VFW59_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEX5363913.1"
                     /translation="MSYLDDMSNDEQHGLLGQIDARTSLAGSNKMEILLFSLGSGEKF
                     GINVFKVKEVCPAGKITRTPNMPSGVHGIVSLRGHVMPVLSLANFMNISAENAHQTMM
                     VAEYSNHILGFLVEKVDRIIRVDWDKVRATEGMLSDKGALITAITELPDGSLISILDV
                     EQILANAFGEGVVGAVERVNSEHDMCVFFADDSMVARRKIAEVLDKMGVKHIQATNGD
                     EAWERLRSMADAAQSTGGDLRDQIQVILTDAEMPQMDGYVLTQHIRSDRRFEGIPVVM
                     HSSLSSEANRAMGRKVGVDYYVPKFDSGVLSSTLRPLLG"
     gene            4943..5341
                     /gene="cheY"
                     /locus_tag="VFW59_06575"
     CDS             4943..5341
                     /gene="cheY"
                     /locus_tag="VFW59_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEX5363914.1"
                     /translation="MGIENTRFLVVDDFSTMRRIVRNLLKELGFTNVQEAEDGVEALA
                     KLRADEFDFVVSDWNMPNMTGIELLRNIRADAKLKHLPVLMVTAEAKKENIIEAAQAG
                     ASGYVVKPFTAATLDEKLKKIFTANPHLNK"
     gene            5350..6069
                     /gene="cheZ"
                     /locus_tag="VFW59_06580"
     CDS             5350..6069
                     /gene="cheZ"
                     /locus_tag="VFW59_06580"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEX5363915.1"
                     /translation="MNSTSASNDSDDLESLFDSIVSANAAESAPAASSASETGGDKVL
                     NQIGHMTRTLHNTLRELGLNKEIEKAASSIPDARDRLNYVATLTEKAAERVLNATEVA
                     QPIMDKVGGESARLAGQWQLLFDKKLDVEQFKSLVMQTQTFLNEVPNHTKATSAQLTE
                     IMMAQDFQDLTGQVIKKITEVTKTMEEQLVSLLLETAPPEVKAEFDTGLLNGPVINAA
                     GRTDVVTTQDQVDDLLESLGF"
     gene            6160..8028
                     /locus_tag="VFW59_06585"
     CDS             6160..8028
                     /locus_tag="VFW59_06585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX5363916.1"
                     /translation="MNDFVGMEELLGDFLLEAGEMLSDVDSKLMELEKRPDDSGLLNE
                     IFRGFHTIKGGAGFLNATELVALCHLTENLFDKLRNRELKLSAELMDVIFAATADVRK
                     MFSALQQNLQPTPAPAEILANLKAALNGEPITGGFVAPNAAVASPSAPQAPAAAPHAG
                     AVPEGVDWNALYGALTGTDIVAAVGATRDAEKIAGAISEEEATKKVVGRRAADVPGSS
                     VGRRDGDVPAKEAKETTIRVDTERLDQVLNLSGEIGLTKNRLNHLRSDILQGRNDVDT
                     LRELDRSVTQLDMLVVNLQNAVMKTRMQPIGRLFKKYPRLARDLARSLGKDVELVLKG
                     EETEMDKTMIEDLNDPLVHLVRNAVDHGVETIEGRIAAGKPEKSLVELSARQEGDHIL
                     ITITDDGRGMRPEVIRNKAIEKGLITNEMANTLDESQCLDLIFLPGFSTKDEISSVSG
                     RGVGMDVVKTNISKLNGSITIISEPGRGSVFTISLPLTLAILPVLILRLGDQSFAVPL
                     SMVREILSVTPEQLQQVSGRATMVVRGEVLPVLPLAQLIGWEASDVPPEVGVLMQFGN
                     NSFILSADGFAGHDDVVIKSLDTFRPKGVAGVTMSSEGDIVLILDIKELLGDVRGH"
     gene            8057..9265
                     /locus_tag="VFW59_06590"
     CDS             8057..9265
                     /locus_tag="VFW59_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5363917.1"
                     /translation="MSKTNPAGRVRWGMLISAGLLTAHALYFRSLDVALADALLFLAL
                     VVTWGYLTKQGGLEEAGREVANGLGDAELIHDSNAFHVQLGKEVSGQLTSAHTELGNT
                     QAILSDAINTLVGTFTALADEVRAQQALTLFITEGGGGDTDGHDSKKKFEHFVQLTSD
                     AMNEFVDSTVQNSKHAMELVEKMDAINAEVGSILRILNEVEGIAKQTNLLALNAAIEA
                     ARAGEAGRGFAVVADEVRNLSESTNKFSKQIRGLVHNVNDALVDAEGAINKLAASDMT
                     FVMDSKQHVQTMMSDLGELNATIAKNAIELHQINAKVERNVGVAVATLQFQDMSSQLI
                     AHAQMRITALQEVANQMSAGADSPNRTEYLQQIATYRDSLHEHVVTLDEKKSNPVAQD
                     NFETGDIELF"
     gene            9405..9770
                     /locus_tag="VFW59_06595"
     CDS             9405..9770
                     /locus_tag="VFW59_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5363918.1"
                     /translation="MAKTVLTVDDSASIRQMVAFTLKSAGYTVIEGVDGEDGLAKAKS
                     NAVNLVLTDQNMPKMDGLTLIKTLRGLPQYRATPILMLTTESSDTMKSQGKAVGATGW
                     LVKPFDPQKLLEVVKKVIG"
     gene            9832..11958
                     /locus_tag="VFW59_06600"
     CDS             9832..11958
                     /locus_tag="VFW59_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5363919.1"
                     /translation="MSIDMSQFYQVFFEESGEHLANMESLLLGLDLDAPDDDQLNAIF
                     RAAHSIKGGSGTFGFTDMTEVTHVLETLLDRIRKHEIAITSEMVDAFLQAGDVLHGLL
                     DVHQNGGVADEAASIDIRARLEKLTRDPVGKGEVEPVAPAPVQEAKSVPSVQHKYQIR
                     FAKTAEIFPAEAQLNNLLEDLANVGKVEDQVIAASEVTLMLTTYMGEAELRDIFSFFL
                     EPEQLTITPQGDVAASASASSLEEDGYGFFVDPGQLQAAIEDEQGFGFFVEPEQLKAE
                     AENAQGYGFFTDVEAPAQVESASTSAAVTPDETPHRRATDKAPAAAETSIRVSVEKVD
                     QMINLVGELVITQSMLAEIASHLDPVLNESLVNGLAQLELNTRELQEAVMSVRMMPIN
                     FVFSRFPRVVRDLAGKLNKKVQLKTVGEGTELDKGLIEKISDPLTHLVRNSLDHGIES
                     PEERIAKGKDPQGTITLRAAHQGGNIVIEVSDDGAGLNRERILAKAREKGIPVSDGMS
                     DADVWQLIFAPGFSTAAVVTDVSGRGVGMDVVKKNIEGIGGRVELSSQFGKGSAVTIR
                     LPLTLAILDGLSVAVGDQIYIVPLSFISESLQPKGAEIKEISGQGKVVHVRGEYLPLI
                     ALHEVFKIKPKVTEPEDGILVLLEGEGKKVALFVDDLLGQHQVVIKSLETNYRRVPGI
                     SGATIMGDGRVAMIMDVGAMIKLAQA"
     gene            12041..12805
                     /locus_tag="VFW59_06605"
     CDS             12041..12805
                     /locus_tag="VFW59_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX5363920.1"
                     /translation="MKIKLLSSLILAALLVPGAALAASPFTANVGLTTDYLVRGISQT
                     SGNPAVQGGVDFVHSSGLYVGVWGSNVSWITDFGATGSASLELDTYAGYRNSFAEDFS
                     YDVGFIRYNYPGTYTPAANTVKADTNEVYGAIGYKWLTAKYSYALGDFLTVANARGTN
                     YLELNASVPVADSGITFGAHYGKQTYRGPSVVGVTALTDPSYSDYKLSVARDWDGYLF
                     TLAYSGTNAPKGGFYTTPAGKDLARGTAVLSVSHSF"
     gene            12853..>13333
                     /locus_tag="VFW59_06610"
     CDS             12853..>13333
                     /locus_tag="VFW59_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5363921.1"
                     /translation="MQDNQGQAADGNNRELLTFTLGSEEYGIDILKVQEIRGYDAVTT
                     IANSPEFIKGVINLRGIIVPIVDMRIKFNLGNVTYNELTVVIILNVANRVVGMVVDGV
                     SDVIALSADQLKAAPEFSGTMDTQYITGLGTVDDRMIIIVDIEKLMSSGDMELIEQVA
                     "
BASE COUNT         2925 a         3395 c         4284 g         2729 t
ORIGIN      
        1 atcgcaatgt ggacgagatg atcggtgaaa ttcgtctggt acaggagtcg gtcaatcaga
       61 tcgccgggtc ggtcaaggag ttcgtcgaga gcacccacgc gatcgccggc atgacgcagc
      121 aggtgaaaga tattgccgag cagaccaact tgctggcctt gaatgctgct attgaagccg
      181 cccgcgccgg cgagcagggg cgcggctttg cggtggtggc ggacgaagtg cgcaagctgg
      241 ccgagaagtc tgcgcagtcg gccagcgaga tcgatcaggt aaccaattcg ctgaaccaga
      301 agtcgaccca cgtcgagggc acggtgcagg caggtctgcg tgcgttgctg acgactcagg
      361 agcatatcga gcgcgtggcg acggtgttga gcgcggcgag tgccgccgtt acacagtcca
      421 gccatggtgt gaatgacatc gcctcgtcgg tggccgaaca gggtttggcg agcaacgaga
      481 tttcgcgcaa cgtggagagg atcgcgcaga tggcggaaga gaaccacgcg gcggtgcaat
      541 cgaatggtca ggagatcgtg cgactggagc aactggctgg cgagttgcgt tcggcggtaa
      601 gccgattcag ggtatagggg cagcgccctg cccgtacggg ggatgcgcgg atttcactgg
      661 ttgagaggta ggcatgcaaa acaagaacgc gattgtgacg aagaacgaga tcccttttcc
      721 agcgggggcg gtgctggtgt cgaagaccga caccaagggg gtcattacct attgcaacga
      781 tgcgttcgag gcgatctcgg gtttctcgcg tgccgagctg atcggcagga gccacaacat
      841 cgtgcgtcac cctgacatgc cgcctcaggc cttcaagtgg ctgtgggaca cgttgaaatc
      901 cgagcgcccc tggcgtggca tggtcaagaa ccggtgcaaa aatggcgact tttactgggt
      961 gcgagccacg gtcgctccca ttatcgaagg cggcaagata atcggttacg tgtcggtacg
     1021 cagggctccg acccgtgcgc agattgccga ggcggaaaag ttctacgccg agttgaatca
     1081 atccggcaga caggtagttt cgaagtacga aaaattaagg atcaagaact ggtcgatgaa
     1141 gggcaagttg caggcagcga tccagatacc gctgctggtg atactttccg tcgcgcagtt
     1201 ctatatcacc ggagcgctca aggatgacgc gctcaggcag gcggcggaca gaggcgatca
     1261 gatcgccaat caggtcatcg atagcgaaaa catgttgatg gtgaccggtc agatcggtga
     1321 cgtggcgacg cgcaaactgt tgctgcataa agtaacttcg tccggtaacg tcaagacggc
     1381 agccgtggtg cgctcgccgc aactgatcga gcagtacgga ggcctgccgg aggaacatat
     1441 cagcgacgat gtgcagcgtc aggtgatcgc gggcaagaag cagagtgtga tgtttgccaa
     1501 agatgccgtc ggcgatccgg tgatgcgcat cgtcacgccc tatctcgcca gcaaggattt
     1561 tcacggcacc gactgcacgt cttgtcacac ggttgccgaa ggcgaggccg ttggcgcctc
     1621 cgatgtggtg atcgatctca agccggactt cgcgcgcatc cattccatgg aactgaacgt
     1681 gctgctggga cagctcggtt tgcaggtgtt cctgtttttc ttcatcggtt actgtgtgga
     1741 tcgctttatt cgcagcccga tggcggatgt gggcagcgag ttcagaaata tcatggaggg
     1801 caatctcgac accgagctgg atatttccat tcatgacgag atgggcgatc tgctgtgcga
     1861 gatacagagc atgcaggctt atttgcgcac catggtggat gagatcgtca cgccggtcac
     1921 ctcgatgcag cgtcgtatcg aggatgtcga cggccgtgtt tccggggtcg ccgccaatgc
     1981 gttgaccgag caggaccata tccagagtat cgccgcgacg atggaagagc tcagccagtc
     2041 gatcaccgag gttgcgaaca tggcgacaga ttccctgagc gatgcgaaag cgatgcagcg
     2101 tatcgtcgaa gagaataacg gcaacatgga gttgagcatt caggcgacga cgcgagtggc
     2161 cgatacggtg cagtcgtcca gtcgcacgat ctccgatctg ggcatttcca tcgagaagat
     2221 cggcgcgata gccaaggcca tcgaggagat cgccgagcag actaacttgc tggcgctgaa
     2281 tgccgcgatc gaggcggctc gcgccggtga gcaggggcgc ggtttcgcag tcgtcgccga
     2341 cgaggtgcgc aagctggccg aacgcaccgc gaccagtacc aaggacatcg cccgcaccat
     2401 cggggagatc acttccattt ccggggctgc ggttaaaacc atgcatgacg ccgtttccga
     2461 agtggaaacg ggtatcggcc tgatacgcaa gaacggcgag ggtttgaagg agatcatgaa
     2521 cgcctccatc aacgtcgcgg gacgcattga gcatatcgcg acggccgcag gagagcagtc
     2581 tcaggcgggc gagggtgtgg cgaatagtct ggaacgaatc aatggtttgg tggacaataa
     2641 cgcacagtcg gccagtgaag ccaagcaggc catggatgaa ttgtcgaaat ctgccagcga
     2701 attgcgcaag gcaggttatc cgttgaccaa gtgtgcgatg gagtgatgcg gcgcgtatgg
     2761 cgccgcgtgg atgctggcgc ctatgtgacc catctgggta tgccgtaggc taaggcagga
     2821 ataatcacag agcctggttg taagaatgag tggttggcga cagatgcacc gtcggtcgca
     2881 agccaagtga ggagaagaaa tgtcggactt gttaagaaat atcgatgcgc gcaccaagct
     2941 ggcgggaacc aataaactgg aaatcctgat gttttcgctt ggaaaggata tgcgaaacgg
     3001 gcgcgaagag accttcggta tcaacgtatt caaggtgcgc gaggtcatgc gcattcccgc
     3061 gatcacgcga gctcccgaga tgccgccggc agtcgagggg atggtgagtc tgcgcgggca
     3121 gttggtgccc gtaatcgatc tggccaagta catcggcatg cagaccgatg gcgaaccgga
     3181 gatcatgatc gttaccgagt acaacggcca cactcagggt ttcctggtgc gggccgtgga
     3241 caatatcctg cgcctggact ggtcgttgat gcgcgtgcct ccggcgatgc tggctgccga
     3301 aatgggcggg ctggtgactg ctgtcaccga attgaaggac aagcggttgg tgatgatgct
     3361 ggacgtggag aaggtgctgg ccgagacggg gcacttcggc gatgacgagg cggtattcaa
     3421 tgccgtgaag ccgctgggca aggagcgcac catcttcttt gccgacgact cgtcggtggc
     3481 gcgcaatcag atttcccgca cgctggatgc catgcaggtg aagcatttgt ccgcagtcaa
     3541 tgggcgtcag gcatgggcgg atctttgcag gctggccgac ttggcggatt cgaccggcgt
     3601 cccgctcaag gagacgattc aggtcattct caccgatgtg gagatgcccg agatggacgg
     3661 ttatatgctg acgcgcaaga tcaagtctga taaacgtttt aacggtattc cggtattgat
     3721 gcactcatcg ctctccagca cttcgaacga gcagatgggg aaggctgtcg gtgtggatga
     3781 gtatgtgccc aagtttgagg cgcagaagtt gtcgcagacg ttggcacggc tgttgagttg
     3841 attcagtgtt gatcgcgatg gggctcgcct catcgctgtg aatgtgtctg ccggctggtc
     3901 tggttgccgg tattaggaga tgggaatgag ttatttggat gacatgtcga acgatgaaca
     3961 gcacgggttg ctggggcaga tagatgcacg caccagcctg gccggttcca acaagatgga
     4021 aatcttgctc tttagcctgg gtagcggcga gaagttcgga atcaacgtgt tcaaggtcaa
     4081 ggaggtatgc ccggcaggca agatcacgcg cacaccgaac atgccgtccg gggtgcatgg
     4141 catcgtctcg ttgcgcggac atgtgatgcc ggtgctgagc ctggcaaact tcatgaatat
     4201 tagcgcggag aatgcgcacc aaaccatgat ggtggccgag tacagcaacc atattctggg
     4261 ctttctggtg gagaaggtcg accgcatcat tcgggtggac tgggacaagg tgcgcgccac
     4321 cgaggggatg ttgtccgaca agggcgcgct gatcaccgcg atcaccgagc tgccggacgg
     4381 ttcgctgatt tcgattctgg acgtcgagca gattctggcc aatgccttcg gcgagggcgt
     4441 ggtgggcgct gtcgagcgag tgaactccga gcatgatatg tgcgtgttct ttgcggatga
     4501 ttcgatggtg gcgcgccgca agatcgccga ggtgctcgac aagatgggcg tcaaacatat
     4561 tcaggcgacg aatggcgatg aggcatggga acgtctcagg tcgatggccg atgcggcaca
     4621 gtctacgggt ggggatttgc gcgaccagat acaggtcatc cttaccgatg ccgagatgcc
     4681 gcagatggac ggttatgtgc tgacgcaaca catcaggtcg gatcgccgtt ttgaggggat
     4741 cccggtggtg atgcattcct cgctgtcttc cgaggcgaac cgtgccatgg ggcgcaaggt
     4801 gggggtggat tattacgtgc ccaagttcga ttccggggtg ttgtcgtcga cattgcgtcc
     4861 tctgctgggt taagaacgat ggatcggtcg tgcgccgatt tgaaacagat ttgcagtatt
     4921 ggttagtatt cgagaggttg gtatgggaat agaaaataca agatttctgg tggttgacga
     4981 tttttcaacg atgcgccgca tcgtgcgaaa tcttctcaag gagctgggct ttaccaatgt
     5041 gcaggaggcc gaagacggcg tcgaggctct ggccaagctg cgcgccgatg agttcgactt
     5101 cgtggtgtcc gactggaata tgccgaacat gacgggcatc gagttgctgc gcaatattcg
     5161 tgcggatgcc aagctgaagc atctgccggt gctgatggtg acggcggaag cgaagaagga
     5221 gaacatcatc gaggccgcac aggcgggcgc ttccggttat gtggtgaagc cgtttactgc
     5281 ggcgacgctg gatgagaagt tgaagaagat atttaccgcc aatccgcatc ttaacaagtg
     5341 agccatgcca tgaactcaac ttcagcaagt aacgactccg acgatctcga atcgttgttc
     5401 gatagcatcg tttcggcgaa tgctgcggaa tccgcaccag cggcttcttc tgcctctgaa
     5461 acggggggcg acaaggtgct caaccagatt ggtcatatga cgcgcacgct gcacaacacc
     5521 ctgcgcgaac tgggcttgaa caaggagatt gaaaaggcgg cgtcgagtat tcccgatgcg
     5581 cgcgatcgct tgaattatgt cgccactttg acggaaaagg ctgccgagcg agtgttgaac
     5641 gccaccgagg tggctcagcc catcatggac aaggtagggg gcgaatcggc gcggttggcc
     5701 gggcagtggc agctgttgtt tgacaaaaaa ctcgacgtgg agcaattcaa gagtctggtc
     5761 atgcagaccc agaccttcct gaacgaggtg cccaatcata ccaaggcaac cagtgcgcaa
     5821 ttgactgaga tcatgatggc gcaggacttt caggatctga cagggcaggt gatcaagaag
     5881 attaccgaag tgaccaaaac catggaagag caactggttt cgttgttgct ggagaccgcg
     5941 ccgccagagg tgaaggccga gttcgatacc gggttgctca acgggcctgt tatcaatgcg
     6001 gcgggacgaa ctgatgtggt gactactcag gatcaagtgg acgatttgct ggaaagcctg
     6061 ggattttaat tacgtagggg cacggcgcgc cgtgcccgta cagattgcgg cgcgccgtgc
     6121 atgcagattg cggtcgtgcc cctacgcaag gagaaaaaaa tgaacgattt tgtcggcatg
     6181 gaagagttgc tcggcgactt cctgttggaa gcgggggaga tgttgtctga cgttgacagt
     6241 aaactcatgg agctggaaaa acgtccggat gatagtggct tgctcaacga gatatttcgc
     6301 ggctttcata ccatcaaggg aggcgcgggt ttcctcaatg cgaccgaatt ggtggcgctg
     6361 tgccatctca ccgaaaatct gttcgacaag ctgcgcaatc gcgagttgaa gctgagtgcg
     6421 gaattgatgg acgtgatctt tgcggcgacg gccgatgtgc gcaagatgtt cagcgcgttg
     6481 cagcagaatc tgcagcctac gccagcgccc gcagaaatat tggctaacct gaaggcggca
     6541 ttgaatggcg agcccatcac gggtggcttc gttgccccga atgccgctgt ggccagcccc
     6601 tccgcgccgc aagctcctgc cgcagcgcct catgcggggg ctgtgccaga gggagtggac
     6661 tggaatgctt tgtacggtgc cctgacgggc accgacatcg tcgccgccgt gggtgcgacg
     6721 cgcgatgcgg aaaagatcgc cggcgcgatc tccgaggaag aggcgaccaa aaaagtggtt
     6781 ggtcgtcgtg ctgccgatgt gccgggcagc tcagttggcc gtcgcgatgg cgatgtgccg
     6841 gccaaggagg ccaaggagac cacgatacgc gtggacaccg agcgtctgga tcaggtgctg
     6901 aatctgtccg gggagatcgg tttgaccaag aatcggttga accatctgcg ctccgatatt
     6961 ctacaggggc gtaacgacgt ggacaccctg cgcgaactgg atcgctccgt cacgcagctg
     7021 gacatgctgg tggtgaatct gcagaacgcg gtcatgaaga cgcgcatgca gcctatcggt
     7081 cgtctgttca aaaaatatcc tcgtttggcg cgtgatctgg cgcgttctct gggcaaggat
     7141 gtcgagctgg tgctgaaggg cgaagagacc gagatggaca agaccatgat cgaggatctg
     7201 aacgacccgt tggtgcactt ggtgcgcaac gcggtggatc atggcgtgga gaccatagag
     7261 ggacgtatcg ccgcaggcaa gccggaaaag agtctggtgg aattgagtgc gcgtcaggaa
     7321 ggcgatcaca tcctgatcac gatcaccgac gatgggcgtg gtatgcgccc cgaggtgata
     7381 cgcaacaagg ccatcgagaa ggggctgatt accaatgaga tggccaatac cctggatgag
     7441 agtcagtgtc tggatctgat cttcctgccg gggttttcga ccaaggatga aatttccagc
     7501 gtgtcgggcc gcggtgtcgg catggacgtg gtgaagacga atatctccaa actcaacggg
     7561 tcgatcacga tcatctccga gcctgggcgc ggaagtgttt tcacgatttc gctgccgctc
     7621 acactggcga ttctgccggt attgatcttg cgtttgggcg atcagtcgtt tgccgtacct
     7681 ctgtctatgg tgcgcgagat tctgtcggtt acgccggagc agctgcagca ggtttccggc
     7741 cgcgcgacca tggtcgtgcg tggcgaagtg ctgccggtgc tgccattggc gcaactgatc
     7801 ggttgggagg ccagcgatgt gccgcccgag gtcggggtgc tgatgcagtt cggcaataat
     7861 agttttatct tgtcggccga tggttttgcc ggccatgacg atgtggtgat caagtcactg
     7921 gataccttcc ggcccaaggg tgtggcgggc gttaccatgt ccagcgaagg cgacatcgtg
     7981 ctgattcttg atatcaagga gttgctcggc gacgtgcgcg gacactgatc caatgtttca
     8041 aagaggatag ccaaatatga gtaaaactaa tccggctggc cgggtgcgat gggggatgtt
     8101 gatcagcgca ggattgctga ccgcccatgc actgtacttc cgcagcctcg atgttgcctt
     8161 ggcggatgcg ttgctgtttc tggctctggt ggtgacctgg gggtatctga cgaaacaggg
     8221 cggcctcgag gaggcgggca gagaagttgc gaatggcttg ggcgatgcgg agctgattca
     8281 cgacagtaat gcttttcatg tgcagttggg caaggaagtg tccgggcaat taacgtccgc
     8341 gcacaccgag ttgggcaaca cacaggcgat actcagcgac gcaatcaaca cgctggtggg
     8401 taccttcacc gccctcgcag atgaagtgcg tgctcagcag gcattgacgc tgttcattac
     8461 cgagggtggt ggaggcgata ccgatgggca tgattcgaag aaaaaattcg aacatttcgt
     8521 gcagctgaca tccgatgcga tgaacgagtt cgtcgacagc acggtgcaga acagcaagca
     8581 tgcgatggag ctcgtggaga agatggatgc gatcaatgcc gaggtcggca gtatcctcag
     8641 aatcctgaac gaggtggagg gtatcgccaa gcagaccaat ttgctggcgc tgaatgcggc
     8701 gatcgaagcg gcgcgtgccg gtgaagccgg gcgagggttt gccgtggtgg ccgacgaggt
     8761 gcgcaatctt tccgagagca ccaataaatt cagcaagcag atccgtggct tggtgcataa
     8821 cgtcaacgat gcgctggtcg atgccgaggg cgcgatcaac aagctggccg cgagcgatat
     8881 gaccttcgtg atggactcca aacagcatgt gcagaccatg atgtcggatt tgggggagtt
     8941 gaatgccacc atcgccaaaa atgccatcga gttgcatcag atcaatgcca aggtggagcg
     9001 caatgttggc gttgccgttg cgacgctgca atttcaggat atgagttctc agttgatcgc
     9061 tcatgcgcag atgcgtatca ctgccttgca ggaagtcgcc aatcagatga gcgctggagc
     9121 cgacagtccg aatcgtaccg agtacctgca gcagatcgca acttatcgcg attcgttgca
     9181 tgagcatgtg gtgacactgg atgaaaagaa atccaatccg gtcgcgcagg ataattttga
     9241 gacgggcgat atcgagttgt tctgacagga cttcgatgcc tcaaattcag ccgcgttctc
     9301 acgtggccaa ttctcggtcg gttgacaggt ttgacccttc attgcatcct tcggccgagc
     9361 aaagtattta cctgaaattt caataaccag aacgagcgag aaaaatggcc aaaaccgtac
     9421 tgaccgttga cgattccgct tccattcgac agatggtggc ttttaccctt aaatccgcag
     9481 gctacaccgt gatcgagggg gtggatggcg aagatggact ggccaaggcc aagtccaacg
     9541 ccgtgaatct ggtgctgacc gaccagaata tgcccaagat ggacggcttg acgctgatca
     9601 agacgctgcg cgggttgccg caataccggg cgacgccgat cctgatgctg actaccgaat
     9661 ccagcgatac gatgaagtcg cagggcaagg ccgtcggcgc aacaggttgg ctggtcaagc
     9721 cattcgaccc gcagaaactg ttggaagtcg tcaagaaggt gatcggctaa gacgtagtag
     9781 gggcacggcg cgccgtgccc ccactttcac atcgcatacc aggggaagcc aatgtctatc
     9841 gacatgagcc agttttatca ggtgttcttc gaggaatcag gcgaacatct tgccaacatg
     9901 gagagcctgc tgcttgggct ggatcttgac gcgcccgacg acgatcagtt gaacgcgatc
     9961 ttccgtgccg cccactccat caaggggggg agcggcacct tcggttttac cgacatgacc
    10021 gaggtgacgc acgtactgga aacgttgctg gatcgtatcc gcaaacatga aatcgcgatc
    10081 acctccgaaa tggtggatgc tttcttgcaa gctggcgacg tactgcatgg tctgctggac
    10141 gtgcatcaga acgggggcgt tgccgatgag gctgcatcga tcgatattcg cgcaaggctg
    10201 gagaagctga ctcgagatcc ggttggcaag ggtgaggtcg agcctgttgc gccagccccg
    10261 gttcaggaag cgaagtcggt tccctccgtg cagcacaaat accagatccg tttcgccaaa
    10321 actgcagaga tattccccgc agaagcgcaa ctcaataacc tgctggaaga cctggcgaat
    10381 gtgggcaagg tcgaggatca agtgattgcc gcaagcgagg tgacgctgat gctcaccacc
    10441 tacatgggtg aagccgagct gcgcgacatc ttttcgttct tcctggagcc tgagcaactc
    10501 acgatcacgc cacagggcga tgtcgcggcc agcgcgtcag cctcctcgct ggaggaggat
    10561 ggttatggtt tcttcgtcga ccccgggcag ctccaggcgg cgatcgagga tgagcagggc
    10621 ttcggtttct ttgtcgagcc cgagcaactc aaggccgagg ccgagaatgc gcagggttac
    10681 ggcttcttta ctgatgtcga ggctccggcc caggttgaat ccgcatcgac ctctgctgcg
    10741 gtaacgccag acgagacacc gcatcgtcgt gcgaccgaca aggctcccgc cgctgccgag
    10801 acctcgattc gcgtcagcgt ggaaaaagtc gaccagatga tcaatctggt cggcgagctg
    10861 gtcattaccc aatccatgct ggcggaaatc gcgtcccatc tggatccggt gttgaacgag
    10921 tcgctggtta acgggttggc gcagctcgag ctgaataccc gcgagctgca ggaagcggtg
    10981 atgtcggtgc gcatgatgcc gatcaacttc gtgttcagcc gcttcccgcg tgtggtgcgc
    11041 gatctggccg gcaagctcaa caagaaggtg cagctgaaga ccgtaggcga gggcaccgag
    11101 ctggacaagg ggctgatcga gaagatcagc gacccgctga cccatctggt gcgcaacagt
    11161 ctggatcacg gtatcgagtc gcccgaggag cgcatcgcca agggcaaaga tccgcaggga
    11221 accatcacgc tgcgtgccgc gcatcagggc ggcaacatcg tcatcgaagt gtcggacgac
    11281 ggcgccggcc tgaatcgcga acgcattctg gccaaggcca gagaaaaagg cattcccgtc
    11341 agcgacggca tgagtgatgc cgatgtgtgg cagctgatct tcgcccccgg cttctccaca
    11401 gccgccgtgg tcaccgatgt gtcggggcgc ggcgtgggca tggatgtggt gaagaagaac
    11461 atcgagggca ttggcggacg ggtcgagctt tcctcgcagt ttggcaaggg cagtgccgtc
    11521 acgattcgcc tgccgctgac actggcgatt ctggatgggc tctcggtcgc cgtgggtgac
    11581 cagatataca tcgtgccgct cagctttatc agcgaatcgt tgcagcccaa gggcgcggag
    11641 atcaaggaga tcagcggaca aggcaaggtc gtgcatgtgc gcggtgagta tctgccgttg
    11701 atcgctttgc acgaggtgtt caagatcaag cccaaggtga ccgagcctga ggacggcatc
    11761 ctggtgttgc tggaaggcga gggcaagaag gtcgccctgt ttgtcgacga tctgttgggc
    11821 cagcaccagg tggtgatcaa gagcctggag accaattacc gcagagtgcc cggtatttcc
    11881 ggcgcgacca tcatgggtga tggccgcgtg gcgatgatca tggatgtggg agcgatgatc
    11941 aagctggcgc aggcgtagtg aacttcgcgg cataacggcc ggtgttctgg ttcgggcagg
    12001 gtaaacctgg cggattattg aattgctaaa ggagcttgtg atgaagatca aactgttgag
    12061 ctccctgatt ttggccgcac tgctggtgcc tggtgcggca ctggcagcat caccgttcac
    12121 cgccaatgtc ggcttgacta ccgattatct ggtgcgcggc atttcgcaga catctggcaa
    12181 tccagccgtg caaggcggcg tcgatttcgt tcattccagt ggtctgtatg tcggtgtgtg
    12241 gggttcgaat gtgagctgga tcaccgactt tggcgcaacc ggtagcgcca gcctggagtt
    12301 ggacacctac gccggttacc gcaacagctt tgccgaagac ttcagctacg atgtcggctt
    12361 catccgctat aactaccccg gcacctatac cccggctgcg aataccgtca aggcggatac
    12421 caacgaggtc tacggcgcga tcggttacaa gtggctgact gccaagtatt cttatgcgct
    12481 gggcgatttt ttgaccgtgg cgaatgccag gggcacgaat taccttgagc tgaatgccag
    12541 cgttcctgtg gccgatagcg gcatcacctt cggtgcgcat tacggcaaac agacctaccg
    12601 tggcccgtcg gtcgttggcg tcacggcttt gaccgatcct tcctattccg actacaagtt
    12661 gagtgtggcc agggactggg atggctacct gttcacactg gcctactcgg gcaccaacgc
    12721 gcccaagggc ggtttctaca ccacgccggc cggcaaggat ttggcgcgag gcaccgctgt
    12781 gttgtcagta tcccattcgt tctgatttgt taacgttcgc ggcgcgcgtc gcactaaaaa
    12841 aggagatgca ccatgcaaga taaccaaggg caggcggcag atggcaataa ccgcgagctg
    12901 ctgacattta cactcggcag cgaggaatac ggcatcgaca tcctcaaggt gcaggagata
    12961 cgcggctacg atgcggtcac caccatcgcc aattcgcccg agttcatcaa gggcgtgatc
    13021 aacctgcgcg gcatcatcgt gcctatcgtg gacatgcgca tcaagttcaa cctcggcaac
    13081 gtgacctata acgaactgac cgtagtcatc atccttaacg tcgcgaaccg cgttgtggga
    13141 atggtggtcg acggcgtgtc ggacgtaatc gcgctgagtg cggatcagct caaggcagct
    13201 ccggaattca gcggcacgat ggatacccag tacatcaccg gactgggtac cgtagacgac
    13261 cgcatgatca tcatcgtcga tatcgaaaaa ctcatgtcca gcggagatat ggaattgatc
    13321 gaacaagtcg cag
//
LOCUS       DASUIV010000180         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_136123, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000180 DASUIV010000000
VERSION     DASUIV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136123"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFW59_06615"
     CDS             complement(<1..800)
                     /locus_tag="VFW59_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363922.1"
                     /translation="MYKPLVPLATAARRWEGAAFWLMLAKAAGLFSAAIGALVLLGWM
                     LDMPMLKSIAPGWVAMKANTAVGFILSGLTLWLKASETSRSNRHWRHVEQALASVTIL
                     LGLFTMLEFQAGIDLGIDHWLFHEPPDAIGTLAPGRMAPATALCFVLLGSALLLDGKT
                     RRSGVSISALALLMALLALAAGLVYLYDTDNLYGLGYAMQLAAHTALGFLVLAAGLLC
                     SLPEHGVVALLRRRDSGGALARRLLPVTLLLPVLIGWLKLEGVRAGLY"
     gene            1222..1905
                     /locus_tag="VFW59_06620"
     CDS             1222..1905
                     /locus_tag="VFW59_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX5363923.1"
                     /translation="MQYQTATQTGTLAIEQNKVLRNTYMMLALTMIPTVIGAFIGMSV
                     NFSFMAEHPIMSALLMFGAMMGMMFAVSALRNSVWGIVALLGFTFVAGVLLGPILQVA
                     LHLKNGAQLVGIAAGGTGIIFFSLATIATVTKKDFSFMGKFLFIGLILIVVASLANMF
                     FQIPALSLTISAAAVMIFSAYILYDVSQIVRGGETNYVMATLGLYLNIYNLFINLLSL
                     LMALSGERE"
     gene            1972..2319
                     /locus_tag="VFW59_06625"
     CDS             1972..2319
                     /locus_tag="VFW59_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HopJ type III effector protein"
                     /protein_id="HEX5363924.1"
                     /translation="MELNTFLQRLNEAPETVAFNDTIAVIEALYDFTPTAFTNGNLRN
                     EAGQNNGSCKLFSFARLNGLSQQQTLACFGAYYRDDVLKHPEATDHQNIRNFMLTGWD
                     GIAFDGVALTPKR"
     gene            complement(2418..>5006)
                     /locus_tag="VFW59_06630"
     CDS             complement(2418..>5006)
                     /locus_tag="VFW59_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5363925.1"
                     /translation="VLTIHYPKSLMRPAIFQNLSIVVALGLLTLLIEIFIIRSVLQNQ
                     VAAPLARLIRATRLVGQSGKPLDKKDLPIDSGDEIGELANSFACMAARVHDTQEALES
                     RVLQRTEELAVVSAQAQRANEAKSLFLANMSHEIRTPMNAIIGMTHLAQRTSPTPKQQ
                     GYLSKIDNAAKSLLAIINDILDFSKIEAGKLELEHSTFALDEVLSSFADIVGHKAEQK
                     GIEIILAVDPDTPRHLVGDSLRLGQILINLVNNAVKFTEQGEIVISVKPEVLSDEQVH
                     LRFSVQDTGIGMTPEQLAGLFQSFSQTDISTTRKYGGSGLGLAISKQLAELMGGRIWA
                     ESVAGQGSTFSFTATLGVAQEVSPTQHLALLGELRGKKILVVDDSDNVREALSVMLGS
                     YDVKVQAVSSGQAALSALSTASLDGTPFDLVLMDWRMPGMDGIETSRRIKAEQTLALT
                     PAILMVSAFEREEVMHQSDAAGLDGFLIKPVSESSLIDSITSLFCGHHAAPEHMPELS
                     ASSHLTGRRVLLVEDNAINRELAIELLADLGISVEIAVNGREGVARATTEEFDLVLMD
                     IQMPEMDGLTATRLIRADERLRDLPVIAMTAQAMSGDREKSLEAGMNDHIVKPIDPKI
                     LTETLSKWMVASPAHPDGAQSAAQVATVRGGDELPEHLPPFDIPAALARASGKSGLLC
                     KMLLSFHDTYADAGAELRRLIAEGKREEAMRLAHSLKGDAGTLEAVAVRDAASAVEYA
                     FRANEMEALASLIDALEEMLIPAVAATDKLKRNKTAIPPSPSTVSFDQAEVVAMSAAL
                     RKLLSLNNTKARKLYASFSEKLRGCGRDEELIELEESLDKFDFSNARLALDRLMEKLD
                     FPEAKL"
BASE COUNT         1067 a         1547 c         1368 g         1024 t
ORIGIN      
        1 tcatacagcc ccgcacgaac cccttccagc ttcagccagc cgatcaagac cggcagcaac
       61 aacgtgacag gcagcagacg tcgggccagc gcgccgccgc tgtcgcgccg acgcagcaat
      121 gcgaccacac cgtgctccgg caaggaacag agcagaccgg ccgcgaggac aaggaagccc
      181 agcgcggtat gcgcagccag ctgcatggca taccccaagc catagaggtt gtcggtatcg
      241 tagagataga ccagcccggc agcgagcgcc aaaagcgcca tcaacagggc aagggcggaa
      301 atactcaccc cgctgcgccg ggtcttgcca tccagcagca atgccgaacc cagcaacaca
      361 aagcacagtg cggtcgctgg cgccatgcgc cccggcgcca gcgtgccgat ggcgtccggt
      421 ggttcatgga acaaccagtg gtcgatgccg agatcaatac cggcctgaaa ttccagcatg
      481 gtaaacaggc cgagcaggat agtgacgctg gccaatgcct gttcgacatg acgccagtga
      541 cggttggatc gagacgtttc gctggccttc agccagagtg tcagacccga cagaatgaaa
      601 cctacggccg tattcgcctt catcgcgacc cagcccgggg cgatactttt cagcatgggc
      661 atatccagca tccagcccaa cagcacgaga gcaccgatgg ctgcactgaa cagccccgca
      721 gctttggcga gcatcagcca gaatgcggca ccctcccagc ggcgcgctgc cgtagctaaa
      781 gggacgaggg gtttatacat tccacaggca gccacgaaca tgcggcatga aaccatccga
      841 agccagccat tccagagcat gttgcgtaac gtgttctatc ttgtgctccc atcattgagg
      901 acaacaaact ggaaactgcg acatcccccg ccggcggaga aatcgcatct taacgtattg
      961 tcatccaacc aaccccaaag aatcggactg cgagcctgtc cggagaagaa gccgacgttc
     1021 cgcatcagaa cctgaatata ttttgcatca tgagtcatgc cggagcgcgc aatgcggatt
     1081 gcgcaattga aactttttct gcacttggtt agtcttatca ccgctgcaat gagaagcacc
     1141 gtaaaatacc gcagccgtca ctacgggtaa cgcagatttt gaagtggcgc agatttttaa
     1201 cgtttaacca ggagttcgca catgcaatac caaaccgcaa cgcaaaccgg cacactcgcc
     1261 atcgaacaga acaaggttct gcgcaacacc tacatgatgc tggcgctgac catgattccc
     1321 acggtcatcg gtgcgttcat cggcatgtcg gtcaatttct cgttcatggc ggagcacccg
     1381 atcatgagcg cgctgctgat gttcggcgcg atgatgggca tgatgttcgc ggtctccgca
     1441 ctgcgcaaca gcgtgtgggg catcgtcgcc ctgctggggt tcaccttcgt cgcgggcgtg
     1501 ctgctcggcc ctatcctgca ggtcgcgctg catctgaaga acggcgcaca gctggtcggc
     1561 atcgccgcag gcggaaccgg catcatcttc ttctcgctgg ccaccatcgc caccgtgacc
     1621 aagaaggatt tcagcttcat gggcaagttc ctgttcatcg gcctgatcct gatcgtcgtc
     1681 gcctcgctgg cgaacatgtt cttccagatc cccgcgctca gcctgaccat ctccgctgct
     1741 gccgtgatga tcttctcggc ctacatcctg tacgacgtca gccagatcgt gcgcggcggc
     1801 gagaccaact acgtgatggc gacgctgggc ctgtacctga acatctacaa cctgttcatc
     1861 aacctgctga gcctgctgat ggcgctcagc ggcgaacgcg aataaggctt catccgcatc
     1921 aagagaaagg cggcttttgc cgccttttgt ttatccgaca ggaatatatc catggaactg
     1981 aatacttttt tgcaacgcct caacgaagcg cccgaaaccg tggccttcaa cgacaccatc
     2041 gcggtgatcg aagcacttta cgacttcacc cccaccgcct tcacgaacgg caacttgcgc
     2101 aacgaagccg gacagaacaa cggctcctgc aaactgttct cgttcgcgag gctcaacggc
     2161 ctgtcgcagc aacagaccct ggcctgcttc ggcgcctatt accgcgacga cgtgctcaaa
     2221 cacccggaag cgacggatca ccagaacatc aggaatttca tgctgacggg ctgggatggc
     2281 attgcgttcg acggtgtagc gctgacgccg aaacgataat cgggattatc gatgccgtca
     2341 ccggaaatat cagcgagcaa ttcgatgttc gtcagcggat cgtcgatcca ccagaatcct
     2401 ggactgcact tgtgtcatta cagctttgct tccgggaaat cgagtttttc catcaagcgg
     2461 tccagcgcca accgcgcatt cgaaaagtca aacttatcca gactttcctc aagctcaatg
     2521 agttcttcat ccctcccgca tccgcgcaat ttttcgctaa aactcgcgta gagcttgcgg
     2581 gccttggtgt tgttgagcga aaggagcttt cttaacgccg ccgacatcgc gacgacctcc
     2641 gcttgatcga aacttacggt cgatggggat ggcggtatgg cggttttgtt ccgcttcagc
     2701 ttgtccgtgg cggcaacggc cggaatcagc atctcttcca gcgcatcgat cagggaggct
     2761 aacgcttcca tctcgttcgc acggaatgcg tattcgacag cggaagccgc atcgcgcacc
     2821 gccacggctt ccagtgttcc ggcatcgccc ttgagcgaat gcgccagccg catggcttcc
     2881 tcgcgcttgc cctccgcaat cagcctgcgc agttccgccc cagcatccgc ataagtatca
     2941 tgaaaactca acagcatttt gcacagcaat cccgacttgc cgctcgccct tgccaaggct
     3001 gcaggaatgt cgaacggagg gaggtgctcg ggaagctcgt ctccaccgcg aaccgtcgcc
     3061 acctgcgcag ccgactgcgc cccatccgga tgcgcgggag atgccaccat ccacttactc
     3121 aacgtctcgg tcagtatttt tggatcgatc ggcttcacga tatggtcatt catgcctgct
     3181 tccagactct tttcccggtc accgctcatg gcctgggccg tcatggcaat gacgggcaga
     3241 tcgcgcaggc gctcatcggc gcggatcaat cgcgtcgccg tcagcccgtc catctccggc
     3301 atctggatgt ccatcagcac cagatcgaac tcttccgtcg tggcacgtgc cacaccttcg
     3361 cgaccattga cggcgatctc gacagaaatg ccgaggtcgg ccagcagctc tatcgccagt
     3421 tcgcggttga tcgcgttgtc ctcgaccagc aacacgcgcc gcccggtcag atgggatgaa
     3481 gcgctcaatt cgggcatgtg ttctggcgct gcgtggtgtc cacagaaaag actagtgatg
     3541 ctgtcgatca gggacgattc gctgacaggc ttgatgagga agccatccag tccggcagca
     3601 tcggattgat gcatgacttc ttcccgttcg aacgcggaga ccatcaggat cgcaggggtc
     3661 agcgcaagcg tttgctcggc cttgatgcgg cgcgaggttt ctattccgtc catcccgggc
     3721 atgcgccaat ccatcaacac caggtcaaag ggtgttccat caagggaagc cgtgctcagc
     3781 gccgacaggg ctgcctgacc ggaagacacc gcctgcacct tgacgtcgta gctgccgagc
     3841 atcacagaca gcgcttcgcg cacattgtcg ctgtcatcga cgaccagtat cttcttgccg
     3901 cgcaactcgc cgaggagagc gaggtgctgc gtcggcgaca cttcctgtgc cacaccgagc
     3961 gtggccgtaa aactgaaggt gctgccttga cccgctacgc tctctgccca gatgcgtccg
     4021 cccatcagtt cggcaagttg tttgctgatg gccagcccca gcccgctgcc gccgtatttg
     4081 cgggtggtcg agatgtcggt ctgggagaac gactggaaca aacctgccag ctgctccggc
     4141 gtcatgccga tgccggtatc ctgcaccgag aagcgcaagt gcacttgctc atcgcttagc
     4201 acttcaggct taacggaaat gacgatctcg ccctgctcgg tgaacttgac ggcgttgttg
     4261 accaggttga tgagaatctg ccccagccgc aaggaatcgc cgaccagatg acgcggtgta
     4321 tccgggtcga ccgctaggat gatctctatg cctttttgct cggctttatg cccaacgata
     4381 tctgcgaagc tgcttagtac ttcgtccagc gcgaacgtcg aatgctccag ttccagtttg
     4441 cccgcttcga tcttggagaa gtccagaatg tcgttgatga tggcgagcag cgactttgcc
     4501 gcattgtcga tcttgctcag atagccctgc tgcttcgggg taggtgaagt gcgctgtgcc
     4561 agatgggtca taccgatgat cgcattcatc ggggtgcgga tctcgtggct catgttggcg
     4621 aggaacaggg atttcgcttc gttagcccgt tgcgcctgtg cgctgacgac agccaattcc
     4681 tcggtgcgct gcaacacccg gctttccaat gcttcctgcg tgtcatgcac gcgtgcggcc
     4741 atacaggcga agctattcgc cagttcgccg atctcatcac ccgaatcgat tggcaagtct
     4801 ttcttatcca gcggtttacc ggattgacct accagacgcg tggcgcgaat caagcgcgcc
     4861 agcggcgctg ccacctgatt ctgcaacacg gaacggatga tgaatatctc gatcagcagc
     4921 gtgagcaggc ccagcgcgac cacgatgctc aagttctgaa aaatggcggg acgcatcaga
     4981 gatttcggat aatggatggt cagcac
//
LOCUS       DASUIV010000181         6672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1066457, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000181 DASUIV010000000
VERSION     DASUIV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066457"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFW59_06635"
     CDS             <1..250
                     /locus_tag="VFW59_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5363926.1"
                     /translation="YVIDETQQVAKDYGAVCTPDFFGFNASMELQYRGRLDASRKEAA
                     PDAARDLFDAMLQVAQTGQGPREQLASMGCSIKWKNEA"
     gene            305..1087
                     /locus_tag="VFW59_06640"
     CDS             305..1087
                     /locus_tag="VFW59_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5363927.1"
                     /translation="MLKAVAAAVRLVAAEEIMPRYLKVTHQHKSDGSLCTDADTAAQA
                     ALIRRLQAIHNVPVLGEEMSAGEQHAIWQAGKDGLWCIDPIDGTSNFVRGLPYFAISV
                     ALLREGKSVLGVVYDPVADELFAGELGKGAFLNGEELIGRDPVGSLSQALASVDLKRL
                     DAKLVLQLATQPPYSSQRNFGASALDWCYTAAGRYDMYLHGGQKLWDYAAGLLILAEA
                     GGHACCIESDDFAQGDIWQRSVIAARDETLFKAWKSWIRAQQ"
     gene            1101..2474
                     /gene="trkA"
                     /locus_tag="VFW59_06645"
     CDS             1101..2474
                     /gene="trkA"
                     /locus_tag="VFW59_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX5363928.1"
                     /translation="MKILILGAGQVGSTVAESLVGEANDITVVDSDSHKLRQLQDRLD
                     LRTLTGNAAHPSVLEAAGIADADMLLAVTQSDEVNLVACKLAASLYNTPTRIARIRST
                     EYLNRPEVFSSDNFCVDFSICPEQILTEYIAKLIEFPEALQVQRFFQGRASLVAVRAF
                     EGGPLVGKPLSFLRTHMPHVETRVAAIFRQDGPIIPEGDTVVQDGDEVFFIAATDNIR
                     SVLKEMRRMDKPAKRVMIVGGGNIGRRLAKTLERDYQVKLIEYNKKVCEKLSGDLKNT
                     LVLNGDGTDEKLMQQEHVDEVDVFCALTNDDENNIMAALLAKQGGARKVIALINRGAY
                     VDLLQGGKIDIVLSPAHVTIGSLLAYVRQGDVAAVHSLRRGAAEALELVAHGDRQSSR
                     VVGRRIEEINLPKGATIGAVVRGAEVIMGHHNTVIEAGDHVIVFVIDKKMVQKVEKLF
                     QVSIGFF"
     gene            2474..3934
                     /locus_tag="VFW59_06650"
     CDS             2474..3934
                     /locus_tag="VFW59_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX5363929.1"
                     /translation="MGRTLTVLHALSLMLVVFSIAYVLPVAAALIDGDGSVLLDFLLA
                     MVWTASAGTLMWMLTRRYKGELSIRHGYLLVVTMWTAMPAFATIPLLLTIPELSFTDA
                     YFETMSGITTTGATVLTGLDTLPPAINIWRHELNWLGGLGIIVLAVAVLPLLGIGGRQ
                     LFKAETPGPMKESALTPRIADTARNLWVVYLGITVACILALKWAGMNWLDAICHAFAT
                     LGLGGFSTHDASVGYFNSPQIEFVLIVFMLLAAMNFATHFMALRGKTLRVYLQDAEAV
                     AMLGVVLASCLGIALFLWWQGTYPGFWTALRHASFNLVSIATDCGFASVDFAQWPIFA
                     PLWMLFLSCIVASSGSTGGGIKMMRTLVLFKQAGREFLLLLHPAAINPMKIGGHVVPN
                     KIVFAVLGFIFLYFMTIVTLTFALLISGLDFISSFTAIIACINNAGPGLGVVGPAGNY
                     QGLTDFQTWICTLAMLTGRLEIFTVLILFTPRFWQR"
     gene            3983..4543
                     /locus_tag="VFW59_06655"
     CDS             3983..4543
                     /locus_tag="VFW59_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5363930.1"
                     /translation="MSEFNLTNHFLIAMPSMQEGMFAGTLAYICEHNENGALGIVINR
                     PISLTLSEMFDQINIPLHQPRLEKMPVYFGGPVQTERGFVLHDTKGEWQSSLRINDKL
                     SLTTSKDILEAIGQGEGPHNLIVTLGYSGWDQGQLEHEISENVWLTVPANEHILFELP
                     PEERLAAAMALLGVDFVSLSDEAGHA"
     gene            4575..5123
                     /gene="ruvX"
                     /locus_tag="VFW59_06660"
     CDS             4575..5123
                     /gene="ruvX"
                     /locus_tag="VFW59_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5363931.1"
                     /translation="MSKTPSGTTPSILNPQSQILSGTLLAFDFGTRRIGIAVGNSLLG
                     RANPLETIDDEKNDNRFARIAALIAEWQPVALVVGLPGNDDGTPHELTALCRRFANRL
                     SGRFRLPVMLVDERYTSLAASSRLNEAGVHGRKQKALLDQYAAQQILQAYFDEPSSGI
                     LIVRDIAKNRDEQPESKEPRSD"
     gene            5211..6164
                     /locus_tag="VFW59_06665"
     CDS             5211..6164
                     /locus_tag="VFW59_06665"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX5363932.1"
                     /translation="MNPQLNKHGELQHLLTTEGLPVRIVRDILDRAATFLDHAEGNEI
                     KKVPLLHGKSVFNVFFENSTRTRTTFEIAAKRLSADVVNLNIGSSSTSKGETLLDTVD
                     NLCAMHADMFVVRHAQSGAAHFIAQHVAPDIHVVNAGDGRHAHPTQALLDMFTIRHYK
                     KEFHNLRVAIVGDVLHSRVARSQIHALTTLGVPEVRVIAPKTLLPTMVENLGVRVYHD
                     MAQGLKDVDVVLMLRLQNERMQGAALPSAQEYFKNYGLTEEKLAYAKDDAIVMHPGPM
                     NRGIEIDSRVADGPRSVILSQVTFGIAVRMAVMSILAGNKQ"
     gene            6161..>6672
                     /locus_tag="VFW59_06670"
     CDS             6161..>6672
                     /locus_tag="VFW59_06670"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227584.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5363933.1"
                     /translation="MNLHIKNGRLIDPKNNLDAMQDVYLAAGKVAAIGTAPAGFVADR
                     VIDASGLIVAPGLIDLAARLREPGYEYMATLESEMEAAVAGGITSLACPPDTEPPLDE
                     PGLVDMLRRRAKSLMRARVYPVGALTLKLEGKRLTEMAELASSGCVAFSQANHPIADT
                     QVLWRALQYAA"
BASE COUNT         1259 a         1950 c         2174 g         1289 t
ORIGIN      
        1 ctatgtgatc gacgagactc agcaggttgc gaaggactac ggcgcggtgt gtacccccga
       61 tttcttcggt ttcaacgcca gcatggagct gcaatatcgc ggccgcctgg atgcctcgcg
      121 caaggaagcg gcgccggatg cggcgcgcga cctgttcgac gcgatgctgc aggtcgcgca
      181 gaccgggcag gggccgcgcg agcagctcgc cagcatgggc tgctccatca aatggaaaaa
      241 cgaggcttga gccgcgatgg cgacgcgtag cgtgaagcgc ccgactaccc gcagcatggg
      301 ggcgatgctc aaggccgtgg ccgcagcggt caggctggtc gctgccgaag agatcatgcc
      361 gcgttatctc aaggtgacgc atcagcacaa gagcgacggc agtctgtgta ccgacgcgga
      421 tacggcagcc caggccgcac tgatccgcag gctgcaggcg atccacaatg tgcccgtgct
      481 gggcgaggag atgtcggccg gcgaacagca tgcgatctgg caagccggca aggacggact
      541 gtggtgtatc gatccgatcg acggcacgtc caatttcgtg cgcggcctgc cctatttcgc
      601 catctcggtg gcactgctgc gtgaaggcaa gagcgtgctt ggcgtggtgt acgacccggt
      661 ggcggatgaa ctgtttgccg gcgaactggg caagggcgcg tttctgaatg gcgaggaatt
      721 aatcgggcgc gacccggtcg ggtcgctcag ccaggcgctg gccagcgtcg atctgaaacg
      781 gctggatgcg aagctggtgc tgcaattggc gacccagccg ccatacagtt cgcagcgcaa
      841 tttcggcgcc agcgcgctgg attggtgcta caccgctgcg ggtcgctatg acatgtatct
      901 gcacggcgga cagaagttgt gggactatgc cgccggtttg ctgatactcg cggaagcggg
      961 cggtcatgcc tgttgcatcg aaagcgacga tttcgcgcag ggcgatatct ggcagcgttc
     1021 ggtgatcgcg gcgcgagacg agacgctgtt caaagcatgg aaaagctgga tacgcgcgca
     1081 acaatgatgc ggaggatgtc gtgaaaatat tgattttggg agcggggcag gtcggctcca
     1141 ccgtggcgga gagtctggtc ggcgaggcca atgacatcac cgtggtggat tccgatagcc
     1201 acaagttgcg ccagttgcag gacaggctgg atctgcgcac gctgaccggt aatgcggccc
     1261 acccatccgt tctggaagcg gcgggcatcg ccgatgccga catgctgctg gcggtgacgc
     1321 agagcgatga ggtcaatctg gtggcctgca agctggcggc cagcctgtac aacacgccga
     1381 cccgcattgc gcgcatccgc tcgaccgaat atctcaatcg tccggaagta ttcagcagcg
     1441 acaacttttg cgtcgatttt tcgatctgcc ccgagcagat tctcaccgaa tatattgcca
     1501 agctgatcga attccccgag gcgctgcagg tgcagcgctt cttccagggc cgggcatcgc
     1561 tggtggcggt gcgtgccttc gagggcggtc cgctggtggg caaaccgctg agttttctgc
     1621 gcactcacat gccgcatgtc gagacgcgtg tcgcggcgat cttccggcag gatgggccca
     1681 tcattccgga aggcgatacc gtggtgcagg atggcgacga ggtgttcttc atcgcggcta
     1741 ccgacaatat ccgcagcgtg ttgaaggaga tgcgccgcat ggacaagccc gccaagcggg
     1801 tgatgatcgt gggcggcggc aacatcgggc gcagactcgc caagacgctg gagcgcgact
     1861 atcaggtcaa actgatcgag tacaacaaaa aggtttgcga aaagctgtct ggcgatctga
     1921 agaacacgct ggtgttgaac ggcgacggaa ccgacgagaa gctgatgcag caggagcatg
     1981 tcgatgaggt tgacgtgttc tgcgcgctga ccaacgacga cgagaacaac atcatggcgg
     2041 cgctgctggc caagcagggc ggcgcgcgca aggtgattgc gctgatcaac cgtggcgcct
     2101 atgtcgatct gctgcagggc ggcaagatcg acatcgtatt gtcgcccgcg catgtcacca
     2161 tcggctcgct gctggcctat gtccgccagg gcgacgtggc ggcggtgcat tcgctgcggc
     2221 gcggtgcggc ggaagcgctt gaactggtcg cccatggcga ccgtcaatca tcccgagtgg
     2281 tggggcggcg tatcgaagag atcaacctgc ccaagggcgc gaccatcgga gcggtcgtgc
     2341 gcggtgcaga ggtcatcatg gggcaccata acacggtgat cgaggcgggg gatcacgtca
     2401 tcgtgttcgt catcgacaag aaaatggtcc agaaggtcga aaaactgttt caggtcagca
     2461 tcgggttctt ctgatgggac gcacgctgac cgttcttcat gcgctgagtc tgatgctggt
     2521 cgtgttcagc atcgcctatg tcctgccggt ggcggctgcg ctgatcgacg gggatggcag
     2581 cgtgctgctg gacttcctgc tggcgatggt gtggacggct tccgccggga cgctgatgtg
     2641 gatgctgacc cggcgctaca agggggagct gtcgatccgc cacggttatc tgctggtggt
     2701 cacgatgtgg accgcgatgc cggccttcgc caccatcccg ttattgctga cgatacccga
     2761 gctgtcgttc accgacgcct atttcgagac catgtccggc atcaccacca cgggggcgac
     2821 cgttctgacg gggctggata cgctccctcc ggcgatcaat atctggcggc atgaactgaa
     2881 ctggctgggc ggcttgggga tcatcgtgct ggcggtggcg gtgctgccgc tgctgggcat
     2941 cggcggacgc cagctgttca aggcggagac gccggggccg atgaaggaat ccgcgctgac
     3001 cccgcgcatc gccgatacgg cacgcaatct gtgggtggtg tatctgggta tcaccgtggc
     3061 ctgtattctt gcgctgaaat gggccggaat gaactggctg gatgcgatat gccatgcctt
     3121 tgccacgctg ggcttggggg gcttttcgac gcacgatgcg agtgtcggct atttcaattc
     3181 gccgcagatc gaattcgtgc tgatcgtgtt catgctgctg gcggcgatga atttcgccac
     3241 gcatttcatg gcattgcgcg gcaagactct gcgggtctat ctgcaggatg ccgaagcggt
     3301 tgcgatgctg ggcgtggtgc tggcgagttg tctgggtatc gccctgttcc tctggtggca
     3361 gggaacctat cccggcttct ggacagcatt gcgccatgcc agtttcaatc tggtgtccat
     3421 cgccacggac tgcggcttcg ccagcgtgga ctttgcccaa tggccgatct tcgcgccgct
     3481 gtggatgctg ttcctgtctt gtatcgtcgc gagctccggt tcgaccggcg gcggcatcaa
     3541 gatgatgcgt acgctggtgc tgttcaagca ggccggtcgc gagttcctgc tgctgctgca
     3601 tccggcggcg atcaatccga tgaagatcgg cggtcatgtg gtgccgaaca agatcgtgtt
     3661 cgcggtgctg ggtttcatct tcctgtattt catgaccatc gtcacgctga ccttcgcgct
     3721 gctgatcagc ggcctcgatt tcatctcgtc gttcaccgcc atcatcgcct gcatcaacaa
     3781 tgccggtccg ggtctgggcg tggtcggccc ggccggtaac tatcaaggac tgacggactt
     3841 tcagacctgg atctgcacgc tggcgatgct gacaggccgt ctggagatct ttaccgtgct
     3901 gatcctgttc accccgcgct tctggcaacg ctaaccatgc cgctcgctat gacgtcgcgc
     3961 gggttgcagc tacaatgcga ccatgtctga attcaatctc accaatcact tcctgatcgc
     4021 catgcccagc atgcaggaag gcatgttcgc cggcacgctg gcctatatct gcgagcataa
     4081 cgagaacggc gcgttgggca tcgtgatcaa tcgcccgatc agtctgacgc tgagcgagat
     4141 gttcgaccag atcaacattc cgctgcatca gccgcgactg gaaaagatgc cggtgtattt
     4201 cggcgggccg gtgcagaccg agcgcggctt cgtgctgcac gatacgaagg gcgagtggca
     4261 atcctcgttg cgcatcaacg acaagctgtc gctgaccacg tccaaggata ttctcgaggc
     4321 cataggccaa ggtgaggggc cgcacaatct gatcgtcacg ctgggttact ccggctggga
     4381 tcaggggcag ctggagcacg agatctcgga aaatgtctgg ctgaccgtgc cggccaacga
     4441 acacatcctg ttcgagctgc cgccggaaga gcgtctggcg gccgcgatgg cgctgctcgg
     4501 cgttgatttc gtctcgctct cggatgaagc cggacatgcc tgatatggca ggaatcagga
     4561 ttgaggaatt gggattgagt aaaacaccaa gcggtaccac tccctcaatc ctcaatcctc
     4621 aatcccaaat cctctccggc acgctgctcg cattcgactt cggcacccgg cgcatcggca
     4681 tcgccgtcgg caacagcctg ctgggtcggg ccaatccgct ggaaaccatc gacgacgaga
     4741 aaaacgataa ccgctttgcg cgcatcgccg cgctgatcgc cgaatggcag ccggtcgcgc
     4801 tggtggtcgg cctgccgggc aatgacgacg gcacgccgca cgaactgacc gcgctgtgcc
     4861 gccgtttcgc caaccgcctc agcgggcggt tccgtttgcc cgtgatgctg gtcgatgaaa
     4921 ggtatacttc gctcgccgcg agttcgcggt tgaacgaggc tggcgtgcat ggccgcaagc
     4981 agaaggcact gctcgaccag tatgccgcgc aacagatatt acaggcgtat tttgatgaac
     5041 catcttcggg aatcctcatc gtccgagaca tcgcgaaaaa tcgtgacgag cagcccgaga
     5101 gcaaggagcc gcgcagtgat tgacgagaaa ataacgggtt ccggcacaga cttgcctccg
     5161 gcatatgtcg gcgccgggcg agcattgatc cggtgcgcga aggagtgtcg atgaatccac
     5221 agctgaacaa acacggcgag cttcagcatc tgctgaccac cgagggcctg ccggtgcgca
     5281 tcgtgcgcga catcctcgac cgtgccgcca ccttcctaga tcacgccgaa ggcaacgaga
     5341 tcaagaaggt gccgctgctg cacggcaagt cggtgttcaa cgtgttcttc gagaacagca
     5401 cccgcacccg aaccaccttc gagatcgcgg ccaagcggct gtcggccgac gtggtcaatc
     5461 tgaacatcgg ctcgtcttcc accagcaagg gcgagaccct gctggatact gtggacaacc
     5521 tgtgcgcgat gcatgccgac atgttcgtgg tgcgccacgc gcagtcgggc gcagcgcatt
     5581 tcatcgcgca gcatgtcgcg ccggacatcc acgtcgtcaa tgccggcgac ggccgccatg
     5641 cgcatccgac gcaggcgctg ctggacatgt tcaccatccg ccactacaag aaggaatttc
     5701 acaacttgcg cgtggcgatc gtcggcgacg tgctgcactc gcgcgtggcg cgttcgcaga
     5761 tccacgcgct gaccacgctg ggggtgccgg aagtgcgcgt gatcgcgccc aagacgctgc
     5821 tgccgaccat ggtcgagaat ctcggcgtgc gggtttatca cgacatggcg cagggtctga
     5881 aggatgtgga cgtggtgctg atgctgcgcc tgcagaacga acgcatgcag ggcgcggcgc
     5941 tgccctcggc gcaggaatat ttcaagaact acggcctcac cgaagagaag ctggcctatg
     6001 cgaaagacga tgccatcgtg atgcatccgg ggccgatgaa ccggggcatc gagatcgatt
     6061 cccgcgtggc cgacggcccg cgctcggtga tcctgtctca ggtgactttc ggcatcgcgg
     6121 tgcgcatggc ggtgatgagc atcctcgcgg ggaataaaca atgaacctcc atatcaagaa
     6181 cggtcgtctg atcgacccga agaacaatct cgatgcgatg caggatgtct accttgcggc
     6241 gggcaaggtc gcggccatcg gcaccgcgcc ggcgggtttt gtcgccgaca gggtcatcga
     6301 tgcgagcggc ctgattgtcg cacccggcct gatcgatctg gcggcgcggc tgcgcgagcc
     6361 gggctatgaa tacatggcga cgctggaatc tgaaatggaa gcggcggtgg cgggtggcat
     6421 caccagcctc gcctgcccgc ccgacaccga gccgccgctg gacgagccgg gactggtcga
     6481 catgctgcga cgccgggcga aatcgcttat gcgcgcgcgc gtctatccgg tcggcgcact
     6541 tacgctcaag ctcgaaggaa agcgcctgac cgagatggcg gagcttgcct ccagtggttg
     6601 cgttgccttc tctcaggcga accatccgat tgccgacacg caagtgctgt ggcgcgcgct
     6661 gcagtacgcg gc
//
LOCUS       DASUIV010000182         8128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_42991, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000182 DASUIV010000000
VERSION     DASUIV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42991"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFW59_06675"
     CDS             <1..482
                     /locus_tag="VFW59_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX5363934.1"
                     /translation="LYGLQSLDFVLMFIPVEPAFMLAISHDSDLWQDAWKKNVLLVSP
                     STLLFVVRTVAHPWRQEQQNRNAQDIASRGAELYDKLAGFVDDLENLGAKLQQAQKAY
                     DGAYGKFTGGRGNVIRQAEMLKELGVKPTKQLPQKLVDLAQDEGLADHGRLPGGADR"
     gene            620..1630
                     /locus_tag="VFW59_06680"
     CDS             620..1630
                     /locus_tag="VFW59_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5363935.1"
                     /translation="MKKTVISTALLGALLSMTAAQAGEFEGSWVGAKLGVNSSNITGL
                     DTKNATTYGIEGGHNWDMGGFLLGADVFADFNNKATHNPGAVSYGSDAYGLDAKLGLE
                     AGKWLPYAKLGYARTNGNGQAGAIGGSDVHLGLGVEYKFAPSWSLAGEYTTAAGKTGV
                     SKLNNNNLTLGVNYYFGAPSAAPAAVAAAVVKEEPKPVAKAAPAPKEAWKIIKEEKPV
                     RIEGASFDTNSAKLKPTADAKLQQVVDFAAKYPEANVEVTGHTDDRGNDRYNQKLSEK
                     RAAAVKSNLVKKGIAADRISAKGLGETMPVADNKTQAGRAANRRVEVQYTVREEKKVR
                     VE"
     gene            1759..3363
                     /locus_tag="VFW59_06685"
     CDS             1759..3363
                     /locus_tag="VFW59_06685"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX5363936.1"
                     /translation="MKLAIAQINSVLGDLPGNVARISQYAEQARAQGARLLLTPELSL
                     CGYSPEDLLLRDGFYLACAQALEELAAKLPDIAVVVGHPLERDGKRYNAASLLRGGRV
                     DATYLKYELPNYAVFDEERYFEHGTEPCIFELDGVRFGLNICADIWEAEAARRARDAG
                     AQVLLVLNASPYAMGKQASRQRVIRERIAESQMAVVYANLVGGQDELVFDGNSFAMDA
                     QGVLSAQGVSFEEQLIMLELRDDLRPVGALIPGLSEEAEVYRALCLGVRDYIGKNRFP
                     GVLLGMSGGIDSALTLVVAVDALGADKVHAVMMPSAYTAQISLDDSREMVKTLGVRYD
                     EFPIAPMLQGYLDTLKTTFSGRAEDTTEENLQARIRGTLLMAMSNKFGSLVLTTGNKS
                     EMAVGYATLYGDMAGGFAVLKDVCKTLVYRLARYRNTLGAAIPERIITRPPSAELRAD
                     QTDQDSLPPYDVLDAILAGYVEQDKSIPEIVAQGYAEADVRRVVHLIRISEYKRRQSA
                     VGIRVTERSFGKDWRYPITVRYQDRF"
     gene            3476..3709
                     /gene="acpP"
                     /locus_tag="VFW59_06690"
     CDS             3476..3709
                     /gene="acpP"
                     /locus_tag="VFW59_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5363937.1"
                     /translation="MSIEQRVKKIVAEQLGVNESEVKSDSSFVNDLGADSLDTVELVM
                     ALEEEFECEIPDEDAEKITTVQQATDYVLAHQK"
     gene            3742..4974
                     /gene="fabF"
                     /locus_tag="VFW59_06695"
     CDS             3742..4974
                     /gene="fabF"
                     /locus_tag="VFW59_06695"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5363938.1"
                     /translation="MSKRRVVITGLGIISPVGIGIPAAWQSIVAGKSGITRISHFDPS
                     QLACQVAGEVKDFDVTQYLSGKEARRMDKFIHFGLVAGMEAFKDSGLEVTEQNAERIG
                     VNIGSGIGGLPMIEDTHNDYLAAGPRKISPFFIPGTIINMISGNLSIMYGLKGPNLAM
                     VSACTTGTHCIGESARIIEYGDADVMVCGGSEATICPLAVGGFSSARALSTRNDDPAT
                     SSRPWDKDRDGFVMGEGAGVLVLEEYEHAKARGAKIYAELAGYGMSGDAYHMTSPSTD
                     GPKRSMLNALRNAGLNPQDVNYVNAHGTSTPLGDKNETEAIKLAFGDHAHKLVVSSTK
                     SMTGHLLGGAGGVESVFCALAVHHQIAPPTINILEQDPECDLDYCANTARDMKIDVAV
                     NNNFGFGGTNGSLVFRKV"
     gene            4989..5813
                     /gene="pabC"
                     /locus_tag="VFW59_06700"
     CDS             4989..5813
                     /gene="pabC"
                     /locus_tag="VFW59_06700"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEX5363939.1"
                     /translation="MLVNGLPGNKISIRDRGLLYGDGVFRTLRVSQGKALHWPLHYLK
                     LQHDCAKLDMTCPGEALLRSELDQVLAQHPDGVVKLIVTRGEGARGYAPPVNVEPTRI
                     WDFAPQPDYPPEWVTHGIKAHLCHLRLSAQPRLACVKHLNRLENVLAAAELREAQAHD
                     GELAEGLLLDSAGHLIEGTRSNLFMVSQGHLVTPDLSRCGVAGIQRGRVMAWALQHGK
                     ALQVRDIGLEEALHADELFVVNSVIELWPIRALGQRHWSHFPLGERIRQALEKEEM"
     gene            5813..6790
                     /gene="mltG"
                     /locus_tag="VFW59_06705"
     CDS             5813..6790
                     /gene="mltG"
                     /locus_tag="VFW59_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5363940.1"
                     /translation="MRRLFALMVFLVLLAAVGGGYYALRPLPLPAVPFAFELKPGSSL
                     TAMARQMRQDGLLDHEQLFIWFVRLFGKSGQIKAGSYALEEPVSPLELLRMISEGQVS
                     QRRIVVIEGWTFRQMRDALNASPDITHDSLSLTDAEILQRIGAPEPHPEGLFFPDTYY
                     FAAGSSDLSIFRRAYQIMQQHLQAGWEAREANLPLQTPYQALILASIVEKETGVESDR
                     SMIAGVFVNRLRQGMLLQTDPTVIYGLGQSFDGNLRRRDLHADTPYNTYTRGGLTPTP
                     IALPGQAALQAALHPAQTNALYFVARGDGSSYFSSSLSEHNQAVNKYQK"
     gene            6787..7389
                     /gene="tmk"
                     /locus_tag="VFW59_06710"
     CDS             6787..7389
                     /gene="tmk"
                     /locus_tag="VFW59_06710"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5363941.1"
                     /translation="MSRFITFEGMDGAGKSTHLAWFADTLRQRGLDVVVTREPGGTPL
                     GEQLREMLLHQTMSMGTEALLMFAARMEHLDQLIKPALQAGKWVISDRFSDASFAYQG
                     AGRGMDWDKLSQLEQWVHGDLQPDLTVFFDVPVEVARERLSNNVALDRFEQEQSDFFD
                     RVRGGYHKRVQQNPHRYAVVDAAQSLADVKRQLEAIIESI"
     gene            7391..>8128
                     /gene="holB"
                     /locus_tag="VFW59_06715"
     CDS             7391..>8128
                     /gene="holB"
                     /locus_tag="VFW59_06715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX5363942.1"
                     /translation="MQKIYPWQENDWARLQELRKRPPHGLLLKGGQGIGKFDLAMNFA
                     QSLLCEQPDEAGFACDACPSCHWFSQGSHPDFRLLQSEAQSQEGEDGEDAKSASGKKP
                     SKQISVDQVRGLADFFGMSAHQGGRRAVVIHPVEAMNSNAANALLKSLEEPPKGLLFI
                     MVSHKPQQLLPTILSRCLSFALTPPDAASAVRWLKEQGVKNPAEALAASGFSPLQAVN
                     MAEQVGSEERSKLLRAIRQPETLDVFAL"
BASE COUNT         1626 a         2171 c         2632 g         1699 t
ORIGIN      
        1 agctctacgg cctgcaatcg ctggatttcg tgctgatgtt catcccggtg gagcctgcct
       61 tcatgctggc gatttcgcat gacagcgatc tgtggcagga cgcgtggaag aagaatgtgc
      121 tgctggtaag cccgagcacc ttgctgttcg tggtgcgcac cgtggcgcat ccgtggcggc
      181 aggaacagca gaaccgcaac gcgcaggata ttgccagccg cggtgccgag ctctacgaca
      241 agctggccgg cttcgtggac gatctggaaa atctgggggc gaagctgcag caggcgcaaa
      301 aggcttacga cggggcctat ggcaagttca ccggcggtcg cggcaatgtg atccgtcagg
      361 ccgagatgct caaggagctg ggggtcaagc ccaccaaaca attgccgcag aaactggtcg
      421 atctggcgca agacgagggt ctggccgatc atggccgttt gccgggcggg gcggatcggt
      481 agattgaaga ttctgttgca aaattaacga atcttccact tatactggtt ggtccggcta
      541 ttttatggtt agtatgctct ttatagggtg ggtttctccc tattccattg catcccattt
      601 acattgtcag gagaaaaata tgaagaaaac tgtaatcagt actgctttgc tgggcgctct
      661 gttgagcatg actgcggcac aggccggcga attcgagggt agttgggtcg gtgccaagct
      721 gggtgttaac agttctaata tcaccgggct ggatactaaa aatgccacga cctacggcat
      781 tgaaggcggc cacaattggg acatgggtgg tttcctgttg ggcgcggacg tgtttgccga
      841 ttttaataac aaggccactc ataatcccgg tgccgtgagc tacggcagtg atgcctatgg
      901 tttggatgcc aagctgggcc ttgaagctgg caagtggctg ccatacgcca agctgggcta
      961 tgcgcgcacc aatggcaatg gtcaggctgg ggcgatcggt ggcagcgatg tgcacctcgg
     1021 gctgggtgtg gaatacaagt ttgcaccaag ctggagtctg gcgggcgagt acacgacggc
     1081 cgccggtaaa actggcgtga gcaagctgaa caacaacaat ctgacactgg gcgttaacta
     1141 ctacttcggc gcaccttctg cggcaccggc ggccgttgcc gctgcggttg tgaaagaaga
     1201 gccgaagccg gttgctaaag cggccccggc gcctaaagag gcatggaaga tcatcaagga
     1261 agagaagccg gtgcgcatcg aaggtgccag ctttgacacc aactctgcca agctgaagcc
     1321 gactgcggat gcaaagctgc aacaggttgt cgacttcgcc gccaaatatc ccgaggctaa
     1381 cgtcgaggtg accggtcata ccgatgatcg cggcaatgat cgctacaatc aaaaactgtc
     1441 cgagaaacgt gcggctgcgg tgaagtccaa tctggtgaag aaggggattg ctgccgatcg
     1501 tatttctgcc aagggtttgg gcgaaaccat gccggtggca gataacaaga cccaggctgg
     1561 acgtgcggcc aatcgtcgcg tcgaggtgca gtacaccgtt cgtgaagaga aaaaggttcg
     1621 cgtcgagtaa gtcgtcgcag caagtcgtgc aagaggcggg ccggaaacgg cccgcctttt
     1681 tgttgtctgc cgcattaaat cgccgggcgg ggcggtggta agataccggc cttgaatcct
     1741 taaccaatca ggctcgtaat gaagctggca attgcacaaa tcaatagcgt gttaggcgat
     1801 ctgccgggga acgttgcccg gatatctcag tacgccgaac aggcaagagc gcagggtgcg
     1861 cgactcctgc tgaccccaga actgagtctt tgcggctact cgccggaaga cctgttgctg
     1921 cgcgacgggt tttatctcgc ctgtgcgcag gcgctggagg agctcgccgc gaagctgccg
     1981 gacatcgccg tcgttgtcgg gcacccgctg gagcgggacg gcaagcgcta caacgcggcc
     2041 tcgctgctgc gcggagggcg tgtcgacgcg acctatctca aatatgagct gccgaactat
     2101 gcggtgttcg atgaagagcg ttatttcgag catggtacgg agccctgcat attcgagctg
     2161 gatggagtgc gtttcgggct gaatatctgt gccgatatct gggaggcgga ggcggcgcgg
     2221 cgcgcacggg atgccggggc tcaggtgctg ctggtgttga atgcctctcc ctatgccatg
     2281 ggcaagcagg catcgcgtca gcgcgtcatc cgtgaacgca tcgccgagag ccagatggcc
     2341 gtggtctacg ccaatctggt cggtggtcag gatgagctgg tgttcgacgg caattcgttt
     2401 gcgatggatg cccagggcgt gctgagcgct cagggcgtct ccttcgaaga gcagttgatc
     2461 atgctggagc tgcgcgacga tctgcgccct gtgggggcgt tgattccagg gttgagcgag
     2521 gaggccgagg tgtaccgggc gctgtgtctc ggggtgcgcg actacatcgg caagaaccgc
     2581 ttccccggtg tgttgctggg catgtccggc ggcatcgact cggcgctgac gctggtggtc
     2641 gcggtggatg cgctgggggc ggacaaggtc catgcggtga tgatgccgtc ggcctatacc
     2701 gcccagatca gcctggacga ttcgcgcgag atggtgaaga cgctgggcgt gcgttacgac
     2761 gaattcccga tcgcacctat gttgcaaggc tatctggata ccctgaaaac gacattttcc
     2821 gggcgtgccg aggataccac cgaggagaat ctgcaggccc ggatacgcgg taccttgctg
     2881 atggcgatgt cgaacaagtt cggctcgctg gtgttgacca ccggcaacaa gtccgagatg
     2941 gcggtgggct acgccacgct gtacggcgat atggcgggcg gcttcgcggt gctgaaggat
     3001 gtctgcaaga ctctggtgta tcgtttggca cgctatcgca atacgctggg ggcggcgatt
     3061 cccgagcgca tcattacccg cccgccgagt gccgagttgc gggccgatca gaccgaccag
     3121 gacagtttgc ctccctacga tgtgctggat gcgattctgg ccggctatgt ggagcaggat
     3181 aagtcgatac cggagatcgt ggcgcagggc tacgcagaag cggacgtgcg ccgggtggtg
     3241 cacttgatcc gcatcagcga atacaagcgt cgccagtctg cggtgggcat ccgtgtcacc
     3301 gaacggagct tcggcaagga ttggcgttac ccgatcaccg tgcgttatca ggaccgtttt
     3361 taggctgttt cgagtccctt gtttttcggg gggtgcaagt agtgatttgg aatttcgggc
     3421 gattttgata caatcgcctc cgctttttta acttaatcag ggtaggagtc ttaaaatgtc
     3481 tattgaacaa cgcgttaaaa agatcgtggc cgaacaactg ggcgtgaatg agtctgaggt
     3541 caagagcgat tcttcgttcg ttaacgatct gggtgcggat tctctggata cggttgaact
     3601 ggtgatggcg ctggaggaag agttcgagtg cgaaattcct gatgaagatg cagaaaagat
     3661 caccaccgtg cagcaagcca ccgattacgt tctggctcac caaaagtaat ttctcccttc
     3721 tgtttttaaa acggagtcag tttgtctaaa cgaagagttg taattaccgg actggggatc
     3781 atttccccgg tcggtatagg gattcccgca gcctggcaga gcatcgtcgc gggaaaatca
     3841 ggtatcaccc gaatcagtca tttcgatccc agtcagcttg cctgccaagt cgcaggcgag
     3901 gtgaaggatt tcgatgtgac gcaatatctg tccggcaagg aagcgcgccg tatggataag
     3961 ttcatccatt tcggtctcgt ggccggcatg gaggcgttca aggattccgg gctggaagtc
     4021 accgaacaga atgccgagcg tatcggcgtg aacatcggtt ccggcatcgg tggtctgccg
     4081 atgatcgagg atacgcacaa cgattatctc gctgccggtc cgcgcaagat ttcgccgttc
     4141 ttcattccgg gcaccatcat caacatgatc tcgggcaatc tgtcgatcat gtacggtctg
     4201 aaagggccga atctggcgat ggtctccgct tgcaccaccg gtacccactg catcggcgaa
     4261 tccgctcgta tcatcgagta cggcgatgcc gacgtgatgg tgtgcggcgg atccgaggcg
     4321 accatttgtc cgctggcggt aggcggcttt tcctccgcac gcgcgctgtc cactcgcaac
     4381 gacgatccgg ccacttccag tcgtccgtgg gacaaggatc gcgacggttt cgtgatgggt
     4441 gagggtgccg gtgtattggt gctggaagaa tatgagcatg ccaaggcacg cggcgcgaag
     4501 atttacgccg agctggccgg ttatggcatg agcggcgacg cttaccacat gacctcgcca
     4561 tccaccgacg ggccgaagcg cagcatgttg aatgcgttgc gcaacgccgg actgaatccg
     4621 caggacgtga attacgtcaa cgctcacggc acttcgaccc cattgggcga caagaacgag
     4681 accgaggcga tcaagctggc gttcggcgac catgcccata agctggtggt cagttcgacc
     4741 aagtcgatga ccggccactt gctgggcggt gccggcgggg tcgagtcggt gttctgcgcg
     4801 ttggcagtac accatcagat cgcgccgccg accatcaaca ttctggagca ggatcccgag
     4861 tgcgatctgg attactgtgc gaacacggca cgggacatga agatcgatgt cgcggtcaac
     4921 aacaacttcg gcttcggcgg aaccaacggc tcgctggtat tccgtaaggt ttaaccttat
     4981 cccacgcgat gctggtcaac ggtctacccg gtaacaagat cagcatccgc gatcggggtc
     5041 ttctttatgg cgacggggtg tttcgcaccc tgcgcgtcag tcaaggcaag gcgttgcact
     5101 ggcccttgca ttatctgaag ctgcaacatg actgtgccaa gctggacatg acctgtcccg
     5161 gggaggcatt gttgcgttcc gaattggatc aggtgctggc gcagcatccg gacggtgtgg
     5221 tcaaactgat cgtcacgcgc ggcgagggcg cgcgcggcta tgcgccgccg gtcaatgtcg
     5281 aaccgactcg catctgggat ttcgcgccgc agcccgacta tccgccggaa tgggtaactc
     5341 atggcatcaa ggcgcacctg tgccacttgc gcctgtctgc gcaaccccgc ttggcctgtg
     5401 tcaagcatct gaaccgtctg gagaatgtgc tggcggcggc ggagttgcgc gaggcgcagg
     5461 ctcatgatgg cgaactggcc gaaggcctgc tgctggatag cgcaggccat ctgatcgaag
     5521 gtacccgcag caacctgttc atggtgtcgc aggggcatct ggtcacgccg gatctgtcgc
     5581 gttgcggtgt ggcgggtata cagcgaggac gggtcatggc ctgggcgctg caacatggca
     5641 aggcgttgca ggtgcgcgat atcgggctgg aagaggcctt gcatgccgac gagttgtttg
     5701 tcgtgaacag cgtcatcgaa ctgtggccga tacgcgcctt ggggcagcgt cactggagcc
     5761 actttccgct cggcgaaaga attcgccagg cacttgagaa agaagagatg tgatgcggcg
     5821 actatttgcc ctgatggttt ttctggtgtt gctcgctgcc gtgggaggcg gatattacgc
     5881 cttgcgtccg ttgccattgc ctgcggttcc gtttgcattc gagttgaagc cgggcagcag
     5941 tctgacggcc atggcacggc aaatgcggca ggacggactg ctggatcatg agcagttatt
     6001 catctggttt gtccgcctgt tcggcaaatc cgggcagatc aaggcgggca gctatgcgct
     6061 ggaagagccg gtatcgccgc tggagctgct gcgcatgatc agcgaggggc aggtcagcca
     6121 gcgccggatc gttgtcattg aaggctggac cttcaggcag atgcgcgatg cgctgaatgc
     6181 cagtcccgat atcacgcatg acagcttgag cctgactgat gcggagatct tgcagcgtat
     6241 cggcgcgccg gagcctcatc cggaaggcct gttttttcca gacacttatt attttgccgc
     6301 tggcagcagt gacctgtcga tcttccgccg tgcctatcag atcatgcagc agcatctgca
     6361 ggcaggatgg gaagcacgag aggcgaatct tccgttgcag acgccgtatc aggcattgat
     6421 tctggcctcg atcgtcgaaa aggagacggg agtcgagagc gaccgcagca tgatcgccgg
     6481 ggtgtttgtg aatcgcttgc gccaaggcat gttgctgcaa accgacccga ccgtgatcta
     6541 cgggctggga cagagtttcg acggcaacct gcgcaggcgc gatctgcatg ccgatacgcc
     6601 ttacaacact tatacccgtg gcgggctgac cccgaccccg attgcgttgc cgggccaagc
     6661 cgcgttgcag gccgcgctgc acccggcgca gaccaacgcg ttatacttcg tggcgcgagg
     6721 ggatggcagc tcctatttct ccagttcgtt gagcgaacac aatcaagccg ttaacaaata
     6781 tcaaaaatga gcagattcat taccttcgaa ggcatggatg gcgcgggcaa gagcacgcat
     6841 ctggcctggt tcgccgatac tttgcggcaa cgcgggctgg atgtagtcgt cacgcgtgag
     6901 ccgggtggaa cgccgttggg cgagcagctg cgcgagatgc tgttgcatca gactatgagc
     6961 atgggcaccg aggcgttgct gatgttcgcc gcgcgcatgg agcacctcga ccagcttatc
     7021 aagcccgcgt tgcaggcggg caagtgggtg atttcggatc gcttcagcga tgccagtttc
     7081 gcctatcagg gagccgggcg tggcatggac tgggacaaac tgagccagct ggagcaatgg
     7141 gtgcatggcg atttgcagcc cgatctgacg gtgttcttcg atgtcccggt cgaggtggcg
     7201 cgcgagcgct tgtcgaacaa tgttgcgctg gatcgcttcg agcaggagca gagcgatttc
     7261 ttcgatcgtg tgcgcggcgg ctatcacaaa cgtgtgcagc aaaatccgca ccgctacgcg
     7321 gtggtcgatg cggcgcaatc tctggctgac gtcaaacgcc agctcgaagc gatcatcgag
     7381 tcgatctgac atgcagaaaa tctatccctg gcaggagaac gactgggcgc gtttgcagga
     7441 gctgcgcaag cggcccccgc acggcttgct gctcaagggc gggcagggca taggcaagtt
     7501 cgatctggcg atgaattttg cccagagcct gttatgcgaa cagccggacg aggcgggctt
     7561 tgcctgcgac gcctgtccat cctgtcactg gttctctcag ggatcgcatc cggactttcg
     7621 tctgctccag tccgaggcgc agagccagga gggcgaggat ggtgaggatg ccaagtctgc
     7681 gtccggcaag aaacccagca agcagatttc cgtcgatcag gtgcgcggtc tggccgattt
     7741 cttcggcatg tcggcgcatc agggcggacg ccgtgcggtc gtcatccatc ccgtggaggc
     7801 gatgaacagc aatgccgcga atgcgctgct gaagagtctg gaagagccgc ccaaggggct
     7861 gctgttcatt atggtgtcgc acaagccgca gcagcttctg ccgaccattc tgagccgttg
     7921 cctgtctttc gccttgacgc ctcccgatgc ggcgtccgca gtgcgctggc tgaaagagca
     7981 gggggtgaag aatcccgccg aggcgctcgc cgcctccggc ttctcgccat tgcaggcggt
     8041 caatatggcg gagcaggtcg gcagtgaaga gcgtagtaag ctgttgcgcg ccatccgtca
     8101 gcccgagacg ctggatgtgt tcgcgctg
//
LOCUS       DASUIV010000183         9865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_415781, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000183 DASUIV010000000
VERSION     DASUIV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415781"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VFW59_06720"
     CDS             <1..907
                     /locus_tag="VFW59_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5363943.1"
                     /translation="GTVYSGEIRARNEAALGFRIGGKLVERLVDAGAVVKAGQALARL
                     DAADASLEAGAADAQYRQAEADIGRYRELRAKGFVSQSALEAKEAQFKAVAAQAGLAR
                     NQADYTVLRADRAGVIAATLADAGQVVGAGQPVVRLAGDGGREVAIAIAESQLAGLRV
                     GAPAEITLWAGEGEETRYSGHLRELSPAADPLSRTYPARVTLDKADARVALGMTARVS
                     FGRKNQDGSFIVPLPALFQQGDRAAVWVVAADRSISLRPVAVAAYRDDGAVIASGLAA
                     GERIVAAGVHKLSAGEKIRVIESTK"
     gene            904..3975
                     /locus_tag="VFW59_06725"
     CDS             904..3975
                     /locus_tag="VFW59_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5363944.1"
                     /translation="MKNRFPNLSAWALTHQQMVLYLIIVLMTAGVISYFKLGRAEDPD
                     FTFKVMVVRTIWPGATAQEVERELTERIEKKLQETPWVDVVQSASKPGESLVFVMLKD
                     YSPKAEVPEAWRQVRKKLDDIRHTLPVGVQGPFPNDEFGDVQINVFALTGDGFDLAAL
                     RRYADRAALELRRVPDVKRVELIGVQPEQIHIDVSPNRLASLGISAMQVADALQKQNA
                     VSPAGFIETGHDRIRLRVSGGLDSVEQIRNADLLVNGQHLRLGDIARVSRGLVDPPAP
                     MMRVAGQPAIGLGIVMAKGGNVIDLGANLKQSMKNISDELPVGVEVHVVANQPEVVHG
                     SIHLFESSLTEAILIVLAVSFLSLGWRTGTVVALSIPLVLAITFFMMKIFGIDLQRIS
                     LGALVIALGLLVDDAIIAVEMMVVKMEQGWDRFKAATFAYTSTAFPMLTGTLITAAAF
                     TPVGFSKSAASEYTVSIFQVVTIALVTSWIVAVVFTPYLGYKLLDPKKLIARAQRHGE
                     DIYDTPFYRRFRTLLTWCLRNRWKVIGGTVLIFVLSMAAFGKFVQKQFFPAASRLELM
                     VDVWLPQGASLKATAQEVERIERMLKGDAAVDYYSSYIGNGAPRFFLSLDQQLFADNF
                     GQFVIVTKDLKAREDLKQRLEQRFAADDYSHLRVRVVRLENGPPIGYPVQFRVLGEDV
                     AQIRVIAEQVAVLMRASEHLQNVNFNWNEKIKSLRVEVDQDRARKLGTSSQEVAQAMQ
                     AWLNGVALTQYREGDQLIDVVWRGGAGEETRSLDRLPDIDIPLAGGRHVPLAQIAKLV
                     PVQEEGIIWRRNRLPAMTVRADMADKVQPATVSVQLNAQLDELRAKLPSGYRIEMGGS
                     VEESAKGQTAIMAVMPLMLVGVFTLLMLQLQSISRSVIVLLTAPLGLIGVALALLIFQ
                     VPFGFVANLGFIALAGMIMRNSVILVDQIRQDEEAGKNQWEAIIGSTVRRFRPIMLTA
                     AAAILAMIPLTRQVFWGPMAVSIMGGLVVATLLTCLFLPALYAAWYRVKEE"
     gene            4096..4335
                     /locus_tag="VFW59_06730"
     CDS             4096..4335
                     /locus_tag="VFW59_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363945.1"
                     /translation="MGYAELISKLEALPSEKRAEVFDFIEFLSARCDAASGKVLPHEE
                     WSDAEFSRMAMQQALRGIEDEPVSYTRSDLRESWQ"
     gene            4332..4679
                     /locus_tag="VFW59_06735"
     CDS             4332..4679
                     /locus_tag="VFW59_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5363946.1"
                     /translation="MKRAGQIVLTAFPYSDLSGNKLRPVLMLRQASRYDDWLVCMVSS
                     QVHQAEAGLDEIINLSDADFGNSGLKSQSVLRLSRLAVLDGALLLGSIGSIDSARLSS
                     VRQRLAKWVAAEI"
     gene            4783..5418
                     /locus_tag="VFW59_06740"
     CDS             4783..5418
                     /locus_tag="VFW59_06740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5363947.1"
                     /translation="MNKIAPTKAETALLEPFAGLTLERIHVPASRAEFAAASAEIRAA
                     GIVGFDTESKPTFVTGDVSEGPHVVQFALHDKAYLFQLHHAEARPFLIELLQSEQLLK
                     VGFGLKSDSGHIRRKFGVEFRAVVDLNRVFSMDGYHKEMGVRAAVALLFNQRFPKSKK
                     VTTSNWSQPQLSSQQLLYAANDAYAALKVLEALNLPRAELPVMGLPQPLQN"
     gene            5552..6328
                     /locus_tag="VFW59_06745"
     CDS             5552..6328
                     /locus_tag="VFW59_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5363948.1"
                     /translation="MTVVAVFNQKGGVGKTTTSLNLAAALARRGYKTTGIDLDPQAQF
                     GSSVGVTAQSGDDSIYGLFQRNRPLHEIIQASSSGIGIIPSHTEMAKVDALYGKGYNA
                     INKLNMSLKVEKTGGDNEHWILDCCPVIGVLSLNAIFASDGLIVPMSADYLSIKGALQ
                     IQKTLKALELVLKRRINRRYLLTRFDSRRRMARDVLETAKKEFGADVCRTLISENVSL
                     AESPLQNKTIFEHAPNSRGAKDYDALLDELLADGFIQQRV"
     gene            complement(6337..6552)
                     /locus_tag="VFW59_06750"
     CDS             complement(6337..6552)
                     /locus_tag="VFW59_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363949.1"
                     /translation="MQKYGFSVKTRNGHLVEHIQISGKDLPDAERKLRQMYMHCEIIQ
                     TQTVSSEKELSQSADIEDVLTLIVKHS"
     gene            6717..7040
                     /gene="fdxA"
                     /locus_tag="VFW59_06755"
     CDS             6717..7040
                     /gene="fdxA"
                     /locus_tag="VFW59_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX5363950.1"
                     /translation="MTYVVAESCIRCKYTDCVDVCPVDCFREGPNFLVIDPDECIDCT
                     LCVAECPAEAIYAEDDLPESQRHFIQLNAELAKKWKPIVAKKPAPPDADQWREVKDKL
                     HLLER"
     gene            7037..9715
                     /locus_tag="VFW59_06760"
     CDS             7037..9715
                     /locus_tag="VFW59_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5363951.1"
                     /translation="MNPNFFHRVAQRILHAQEAPDLRAATILLPNYHAAQPLVQALGA
                     IANTPILLPRMVTFTDWAQTVTLDSPIQPDTRRIAALYQALRERDWFGDADLWSLSRE
                     LLALMDELTRHHVALPESETEFAEQLVEAYRARSGQALQFEARVVHELWFAMTSGEDM
                     DAVSAYQQRLARLVESVDGPLYVLQTCELAAPERRFLDACRERVAVEVFDVRAMAADT
                     PDCALLSRALQRDADSADLKSDAARLQHQPQAKLGARLQLFAAQGLEQEAQAADVQVR
                     RWLLEGKQSIAVVVQDRLVARRARALLERAQVQVRDETGWTMATLSVSTVLMRWLEAV
                     QGEFYYQDVLDLFKSPFLFADAPAERKQAAFLFEQLVRRHGTVAQLESFIELAEREER
                     ELVQPLVRLRQAAQALSRKTASLSEWLAALHDSLEILGVLPGWAQDAAGQQLAQALTQ
                     WAEELHEDGTRCSFSEWRRWLAQQLDLNTFRDFAVESPVLFTHLAATRWRSFDAVLLL
                     GCDAAHLPAPANAGQWFNDAVRSSLGLPVSGVQQAQVRDDLLALLALNGTVMASWQES
                     RNGEPNLLSPHLEMLRALHLLAYRDDLAAGELGAMLNAARVQSEAFALPPANEMPRPA
                     AQPALIPSHISPSGYNSLVACPYQFYARHLLRLNELDEVREELDKRDYGTWVHAALQR
                     FHDEFNVLLEHGREQLEAGLRRISTEVFAEALAHDYLAQAWLLRWQAQIPAYLDWQLA
                     CERDGWRYSGAETPFSFNVAENLILRGRIDRIDVQAEDAEVLRVLDYKTQAVAPLRNK
                     LKDAGEDVQLACYAFDLHAGEAAFVALDGAKVDAVQPPHDINELAQLNIARLKSVFER
                     MGQGEAMPAHGAEQVCRYCEMNGLCRHRQWQEAARG"
     gene            9669..>9865
                     /locus_tag="VFW59_06765"
     CDS             9669..>9865
                     /locus_tag="VFW59_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5363952.1"
                     /translation="MAYAVIGSGRRRRVDNRTALDPKRSVVVEACAGSGKTWLLVSRI
                     VRLLLDGVPPGEILAITFTRRA"
BASE COUNT         1748 a         2939 c         3354 g         1824 t
ORIGIN      
        1 tggcactgtc tacagcggcg agattcgcgc gcgcaacgag gcggcgctgg gtttccgcat
       61 cggcggtaag ctcgtcgagc ggctggtgga tgcgggggcg gtggtcaagg ccgggcaggc
      121 gctggcgcgg ctcgatgcgg cggatgcgag cctggaggct ggggcggcgg atgcgcagta
      181 ccggcaggcc gaggccgaca ttgggcgtta ccgcgagttg cgtgcgaagg gcttcgtgag
      241 ccagtcggcg ctggaagcga aggaggccca gttcaaggct gtcgcggcac aggccgggct
      301 ggcgcgaaat caggccgact acaccgtgtt gcgcgccgat cgtgcgggcg tgatcgcggc
      361 gacgctcgcg gatgcggggc aggtggtggg cgcggggcaa cccgtggtgc gcctggcggg
      421 cgacggggga cgtgaggtcg ccatcgcgat cgcggaatcg cagctggccg gactgagagt
      481 cggcgcgccc gccgaaatca cgctgtgggc gggcgagggc gaggagacgc gttacagcgg
      541 gcatctgcgc gaactctcgc ccgcagccga tccgctcagc cgcacctatc cggcgcgcgt
      601 gacgctggat aaggccgatg cgcgcgtggc gctgggcatg accgctcgtg tcagcttcgg
      661 cagaaaaaat caggatggca gtttcatcgt gccgttgccg gcgctgttcc agcagggcga
      721 tcgggctgcg gtgtgggtcg tcgcggcgga ccgcagcatc agcctgcggc cggttgcggt
      781 cgcggcctat cgcgacgacg gcgcggtgat cgcgagcggt ctggcggcgg gggagcgcat
      841 cgtcgccgcc ggcgtgcaca agctcagcgc gggcgagaag attcgcgtca tcgaaagcac
      901 gaaatgaaaa atcgttttcc caatctttcc gcctgggcgc tgacgcacca gcagatggtg
      961 ctgtatctga tcatcgtgct gatgacggcc ggggtgatct cctatttcaa actcgggcgc
     1021 gcggaagatc cggacttcac cttcaaggtg atggtggtgc gcaccatctg gccgggcgcg
     1081 accgcgcagg aggtggagcg cgagctgacc gaacgcatcg agaagaagct gcaggagacc
     1141 ccgtgggtgg acgtggtgca gtcggcctcc aagccgggcg aatcgctggt gttcgtgatg
     1201 ctcaaggatt actcgccgaa agccgaagtg ccggaagcct ggcggcaggt gcgcaagaag
     1261 ctggacgaca tccgccacac cttgccggtc ggcgtgcagg gacctttccc caacgacgag
     1321 ttcggcgatg tgcagatcaa cgtgttcgcg ctgaccggcg acggattcga cctcgcggcg
     1381 ttgcgccgct atgccgaccg tgccgcgctg gagctgcgtc gtgtgccgga cgtgaagcgc
     1441 gtcgagctga tcggcgtgca gcccgagcag atacatatcg acgtgtcgcc gaaccggctg
     1501 gcgagtctgg gcatcagcgc gatgcaggtg gccgatgcgc tgcagaaaca gaatgcggtg
     1561 agtccggcgg gctttatcga gaccggccat gaccgcatcc gcctgcgcgt gagcggcggg
     1621 ctggacagcg tcgaacagat acgcaacgcc gatctgctgg tgaacggcca gcatctgcgt
     1681 ctgggcgaca tcgcccgcgt cagccgcggt ctggtcgatc cgcccgcgcc gatgatgcgc
     1741 gttgcggggc agcccgcgat cggcctcggc atcgtgatgg ccaagggcgg caacgtcatc
     1801 gacctcggcg cgaacctgaa gcagtccatg aaaaacatct cggacgagct gccggtcggc
     1861 gtcgaggtgc atgtggtcgc caaccagccc gaggtggtgc atggctccat ccatctgttc
     1921 gagagttcgc tgaccgaggc gatcctgatc gtgctggcgg tgtccttcct gagcctgggc
     1981 tggcgcaccg gcacggtggt ggcgctgtcc attccgctgg tgctggcgat caccttcttc
     2041 atgatgaaga tattcggcat cgacctgcag cgcatttcgc tgggcgcgct ggtgatcgcg
     2101 ctcggcctgc tggtggacga tgcgatcatc gcggtggaga tgatggtggt gaagatggag
     2161 cagggctggg accgcttcaa ggctgccacc ttcgcctata cctcgaccgc gtttccgatg
     2221 ctgaccggca cgctgatcac cgccgccgcg ttcacgccgg tgggattttc caaatcggcg
     2281 gcgagcgaat acaccgtctc gatctttcag gtggtgacca ttgcgctggt gacctcgtgg
     2341 atcgtcgcgg tggtgttcac gccctatctg ggctacaagc tgctcgatcc gaagaagctg
     2401 atcgcccgcg cgcagcggca cggcgaggac atctacgaca cgccgttcta ccgccgcttc
     2461 cgcacgctgc tgacctggtg cctgcgcaac cgctggaagg tgatcggagg cacagtgctg
     2521 atctttgtgc tgtcgatggc tgcgttcggc aaattcgtgc agaagcaatt cttccccgca
     2581 gccagccggc tggagctgat ggtggacgtt tggctgccgc agggcgcgtc gctcaaggcg
     2641 accgcgcaag aggtggagcg catcgagcgg atgctcaagg gtgacgccgc cgtggattac
     2701 tactcttcct atatcggcaa cggtgcgccg cgtttcttcc tgtcgctcga ccagcagctg
     2761 ttcgccgaca acttcggcca gttcgtcatc gtgaccaaag acttgaaggc gcgcgaagac
     2821 ctcaagcagc ggctggaaca gcgcttcgcg gccgacgact attcgcatct gcgcgtgcgc
     2881 gtggtgcggc tggagaacgg cccgccgatc ggctatccgg tgcagttccg cgtgctgggc
     2941 gaggatgtcg cgcagatacg cgtcattgcc gaacaggtgg cggtgctgat gcgtgccagc
     3001 gagcacttgc agaacgtgaa ctttaactgg aacgagaaga tcaagagcct gcgcgtcgag
     3061 gtcgatcagg accgcgcgcg caagctcggc acctccagcc aggaagtcgc gcaggccatg
     3121 caggcctggc tgaacggcgt cgcactcacc caatatcgcg agggcgacca gctgatcgac
     3181 gtggtgtggc gcggcggcgc gggcgaggag acgcgttcgc tggacaggct gcccgacatc
     3241 gatattccgc tggctggcgg gcgtcacgtg ccgctggcgc agatcgcgaa actcgttccg
     3301 gtgcaggaag agggcatcat ctggcggcgc aaccgcctgc cggcgatgac cgtgcgcgcc
     3361 gacatggcgg acaaggtgca gcccgcgacg gtatcggtgc agctcaacgc gcagctggac
     3421 gagttgcgcg ccaaactgcc cagcggctac cgcatcgaga tgggcggcag cgtcgaggaa
     3481 tccgccaagg ggcagaccgc gatcatggcg gtgatgccgc tgatgctggt cggcgtgttc
     3541 acgctgctga tgctgcagct gcagagcatc agccgcagcg tgatcgtgct gctcaccgcg
     3601 ccgctcggtt tgatcggcgt cgcgctcgcg ctgctgatct ttcaggtgcc gttcggcttc
     3661 gtcgccaacc tcggtttcat cgcgctggcc ggcatgatca tgcgcaactc ggtgattctg
     3721 gtggaccaga ttcgtcagga cgaagaggcg ggcaagaacc agtgggaagc catcatcggc
     3781 tccaccgtgc gccgcttccg ccccatcatg ctcaccgccg cagccgccat cctcgcgatg
     3841 atcccgctga cccgtcaggt gttctgggga ccgatggcgg tcagcatcat gggcggactg
     3901 gtggtggcga ccttgctgac ctgcctgttc ctgcccgcgc tgtatgcggc gtggtaccgg
     3961 gtgaaggaag aataggattt gggatcgagg atttgggatt ttggatcgag cctgttccgg
     4021 ttaaaggccg tagaaccgtt ggtcgtggaa atcatttatc atctcccgcc atagggatgt
     4081 tgccaggagg gcgagatggg ctatgcggaa ttgatcagta agctggaggc gctgccgagc
     4141 gagaagcgcg cggaggtgtt cgactttatc gaattccttt cggcgcgttg cgatgcggca
     4201 tccggcaagg tgttgccgca tgaagaatgg tcggatgccg agttttccag aatggcgatg
     4261 cagcaggcgc tacgcggcat cgaggacgaa cccgtttctt atacacgcag cgacctgcgc
     4321 gaaagctggc aatgaagcgg gccggacaaa tcgtcctgac ggctttccct tattccgatc
     4381 tttccggaaa caagcttcgt cccgtgctga tgttgcgaca ggcatcccgc tatgacgatt
     4441 ggctggtatg catggtttcc tcgcaagtgc atcaggccga agccggactg gatgagatca
     4501 tcaatttatc cgacgcagac tttggcaatt ccggcttgaa gtcgcagagc gtgttgcgcc
     4561 tctcgcgtct ggctgttctg gatggtgcgc tgctgctagg cagcatcgga agcatagact
     4621 cagcgcggct gagttccgtg cgccagcgtt tggcaaagtg ggtggccgcc gaaatttagt
     4681 ccgcctgagt cgccatcgcg cctgatcgag tccagtcgca ggtgactgtc gagagggccg
     4741 aaagcaagcc cattgtccaa ttcaaatatc agtctttcct ctatgaacaa gatcgcaccg
     4801 accaaggccg agaccgcgct gctggagccg ttcgccggac tcacgctgga acgcatccat
     4861 gtgcccgcca gccgggccga gttcgctgct gcctccgccg agatcagggc ggcaggcatc
     4921 gtcggcttcg ataccgaatc gaagccgacg ttcgtcacgg gcgatgtcag cgaggggccg
     4981 catgtggtgc agttcgcgct gcacgacaag gcctatctgt ttcagctcca tcatgcggag
     5041 gcgcgcccgt ttttgatcga actgctgcaa tcggaacagc tgctcaaggt cggcttcggg
     5101 ctcaagtccg atagcggtca tatccgcagg aagttcggcg tcgagttccg tgcggtggtc
     5161 gacctcaaca gggtgttcag catggacggc taccacaagg aaatgggcgt acgtgcggcg
     5221 gtggcgctgt tgttcaacca gcgcttcccc aagtcgaaga aggtcaccac ctccaactgg
     5281 tcgcagccgc aactttcctc gcagcaattg ctctacgcgg cgaacgatgc ctatgcggcg
     5341 ctcaaggtgc tggaggcgct gaacctgccc cgcgcagaac tgcccgtgat gggcttgccc
     5401 cagccgttgc agaattgagg gggcaggcca gcctcgaaat tgacgtgaat tgatccggtg
     5461 ccgatcctgc ttgcgcaggt ttcggcaaag acactgcagc gctctgctag aatgcccccc
     5521 actgtcatac gaagccgact ccccagtaat catgactgtc gtcgctgtat tcaatcagaa
     5581 gggtggggtg ggaaaaacca ccacgtcgct caatcttgcc gccgccttgg cgcggcgcgg
     5641 ctataaaacc accggcatcg atctggatcc gcaggcgcag ttcggttcct ccgtcggcgt
     5701 gacggcccaa tcgggcgatg acagcatcta cggtctgttc cagcgtaacc gtcccctgca
     5761 tgaaataatt caggcctcgt cgagcggcat cggcatcatc ccgtcgcaca ccgagatggc
     5821 caaggtggat gccttgtacg gcaagggtta caacgcgatc aacaagctca acatgagtct
     5881 gaaggtggaa aagaccggag gcgataacga gcactggata ctcgactgtt gtccggtgat
     5941 cggcgtgctg tcgctgaatg cgatcttcgc cagcgacggg ctcatcgtgc cgatgtcggc
     6001 ggattatctt tccatcaagg gagcgctgca gattcagaag acgctgaagg cgctcgagct
     6061 ggtgctgaag cgccgcatca acaggcgtta tctgctcacg cgtttcgact cgcgccggcg
     6121 catggcccgg gacgtgctgg agacggcgaa aaaggaattc ggagcggatg tctgccggac
     6181 actgatatcg gagaatgtca gcctggccga aagcccgctg cagaacaaga ccatcttcga
     6241 gcatgcgccc aacagtcgcg gtgccaaaga ttacgatgcg ctgctggatg agttgctggc
     6301 ggacgggttc atccagcaac gcgtttgaaa ccggattcag ctgtgtttga cgatcagcgt
     6361 cagcacatct tcgatatccg ccgattgcga cagctccttc tcggagctga cggtctgggt
     6421 ctggatgatc tcgcagtgca tgtacatctg gcgtagttta cgttccgcat ccggcaaatc
     6481 cttgccggat atctggatgt gctcgaccag gtgaccgttc cgcgtcttca cggagaaacc
     6541 gtatttttgc atcgcttcct cggggaaatc atgacaaccg cagagtagag gcattgcagc
     6601 gggggcgtca agcggggcgg atattggctg ccgcgcattg gtcgcggcag agggctttgc
     6661 tagaatgccg tgctggtgga agcatgccgg gactgaaaca gtattggaga atagatatga
     6721 cctatgtggt tgccgagagc tgtatccggt gcaagtacac cgactgcgtg gacgtgtgtc
     6781 cggtggactg cttccgcgag ggaccgaatt ttctggtgat cgaccccgat gagtgtatcg
     6841 actgcaccct gtgcgtggcc gaatgtccgg ccgaggcgat ctatgccgag gacgacctgc
     6901 cggagagcca gcgtcacttc atccagctga atgccgaact ggcgaaaaag tggaagccca
     6961 tcgtcgcgaa gaagcccgca cctcccgatg ccgaccagtg gcgcgaggtc aaagacaaac
     7021 tgcatctgct ggaacgttga acccgaattt ttttcatcgg gtcgcgcagc gcatcctgca
     7081 tgcgcaggag gcgcccgacc tgcgtgcggc gaccatcctg ctgccgaact atcatgccgc
     7141 ccagcccttg gtgcaggcgc tgggggcgat tgcaaacacc cccatcttgt tgccgcgcat
     7201 ggtgaccttc accgactggg cgcaaaccgt cacgctcgac tccccgatac aacccgacac
     7261 caggcgcatc gcagcgctgt atcaggcgct gcgcgaacgc gactggttcg gcgacgccga
     7321 tctgtggagc ctgtcgcgcg aactgctggc gctgatggac gaactcacgc gccatcatgt
     7381 cgccttgccc gagtccgaaa ccgaattcgc cgaacagctg gtcgaggcct accgcgcccg
     7441 cagcgggcag gcgctgcagt tcgaggcgcg cgtggtgcac gagctgtggt tcgcgatgac
     7501 ctccggcgag gatatggatg cggtgagcgc ctaccagcag cgtttggcgc ggctcgtcga
     7561 atcggtcgac gggccgctgt atgtgctgca gacctgcgaa ctcgccgcgc ccgagcggcg
     7621 ctttctggat gcctgtcgcg agcgtgttgc ggtcgaggtg ttcgatgtgc gcgccatggc
     7681 ggcggatacg cccgactgcg cgctgctgtc gcgcgccttg cagcgcgacg cggacagcgc
     7741 cgatctgaag agcgacgcgg cacggttgca gcatcagccg caggcgaagc tgggggcacg
     7801 cctgcagctg ttcgccgcgc aggggctgga gcaggaggcg caggctgccg atgtgcaggt
     7861 gcgccgctgg ctgctggaag gcaaacaatc catcgcggtg gtggtgcagg acaggctggt
     7921 cgcgcgccgt gcgcgtgcct tgctggagcg cgcacaggtg caggtgcgcg acgaaaccgg
     7981 ctggacgatg gcgacgctgt cggtcagcac ggtgctgatg cgctggctgg aggcggtgca
     8041 gggcgagttc tattatcagg acgtgctcga tctgttcaag tcgcctttcc tgttcgccga
     8101 tgcgcccgcc gagcgcaagc aggccgcctt tctgttcgaa cagctggtgc gtcgtcacgg
     8161 caccgtcgcg caactggaaa gcttcatcga gctggccgaa cgcgaggagc gcgagctggt
     8221 gcaaccgctg gtgcgcctgc gtcaggcggc gcaggcactg tcgcgcaaga ccgcgtcgct
     8281 atccgaatgg ctggctgcgc tgcatgacag tctggagata ttaggcgtgt tgccgggctg
     8341 ggcgcaggat gcggcaggcc agcaactggc ccaggcgctc actcaatggg cggaagagct
     8401 gcatgaggac gggacgcgtt gcagcttttc cgaatggcgg cgctggctcg cgcagcagct
     8461 cgacctgaac accttccgcg acttcgcggt ggaaagcccg gtgctgttca cccacttggc
     8521 cgcgacgcgc tggcgcagct tcgatgcggt gctgttgcta ggctgcgatg cggcgcatct
     8581 gcccgcgcct gcgaatgccg gacagtggtt caacgatgcg gtgcgttctt ccctcggcct
     8641 gccggtgagc ggcgtgcagc aggctcaggt gcgcgacgac ctgctggcct tgctggcgct
     8701 gaacggcacc gtgatggcga gctggcagga aagccgcaac ggcgagccga atctgctcag
     8761 ccctcatctg gaaatgctgc gcgcgctgca cctgctggcc tatcgcgacg atctcgccgc
     8821 cggtgagttg ggcgcgatgc tgaatgccgc gcgagtgcag agcgaggcgt tcgccttgcc
     8881 gccggcgaac gagatgccgc gtcctgccgc gcaacccgca ctgattcctt cgcatatctc
     8941 gccttccggc tacaacagtc tggtcgcctg cccctaccag ttctatgcgc gccacctgct
     9001 gcgtctgaac gagctggacg aggtgcgcga ggaactcgac aagcgcgatt acggcacctg
     9061 ggtacacgcc gccttgcagc gcttccatga cgaattcaac gtgctgctgg agcatgggcg
     9121 cgaacagctc gaagcggggc tgcgtcgcat cagcacggag gtgttcgccg aagcgctggc
     9181 gcacgactat ctggcgcagg cctggctgtt gcgctggcag gcgcagatac cggcttatct
     9241 ggactggcag ctggcctgcg agcgcgacgg atggcgctat tccggggccg agactccgtt
     9301 ctcgttcaat gtcgcagaga atttgatcct gcgcggacgc atcgaccgca tcgatgtgca
     9361 ggcagaggat gcggaggtgc tgcgggtgct ggattacaag acgcaggcgg tcgcgccgct
     9421 gcgcaacaag ctgaaggatg cgggcgagga tgtgcagctg gcctgttacg ccttcgacct
     9481 ccatgcgggc gaggccgctt tcgtcgcact ggacggtgcc aaggtcgatg cggtgcagcc
     9541 gccgcatgac atcaatgagc tggcgcagct gaacatcgcg cgcctgaaga gcgtgttcga
     9601 acgcatgggg cagggcgagg ccatgcccgc gcacggcgcg gagcaggtat gccgctattg
     9661 cgagatgaat ggcctatgcc gtcataggca gtggcaggag gcggcgcgtg gataaccgca
     9721 ccgccctcga tccgaaacgc agcgtcgtcg tcgaggcctg cgcgggcagc ggcaagacct
     9781 ggctgctggt gtcgcgcatc gtgcggctgc tgctcgacgg cgtgccgccc ggcgagattc
     9841 tcgcgatcac cttcacccgc cgcgc
//
LOCUS       DASUIV010000184         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_879329, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000184 DASUIV010000000
VERSION     DASUIV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879329"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VFW59_06770"
     CDS             <1..847
                     /locus_tag="VFW59_06770"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX5363953.1"
                     /translation="SDGSEALEALASEEKAFANWLKRNVKPHKLPGYAVVVLSLKKPG
                     EAPGDVSAEQFDALADLADRYSFGELRVTHMQNLVLADVKQSDLVELWYAASEQGLAT
                     PNIGLLTDVICCPGSDFCNLANARSIPLATAVSNRFESIDFQHDIGDIELNISGCINA
                     CGHHHVGHIGILGVDKNGEDRYQVSIGGQQGNGLRLGKVIGSSFSAAETPEVIASLLD
                     LYVQERHEDERFVDTVQRLGVQPFKEHVYKTATAVALGLADDAALHEEAVVASYDDPY
                     YSRRF"
     gene            852..1379
                     /locus_tag="VFW59_06775"
     CDS             852..1379
                     /locus_tag="VFW59_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX5363954.1"
                     /translation="MIIMRESIIRNGSIAQDDWGVLRLDEGDTPEGVEVPQEKIIVPL
                     KVWQAQREKLQHTDLGVWLDSDERAEELAGDLDRFAVIAVNFPKFADGRGYTLAYRLR
                     VRLGYRGELRAIGDVLRDQLFYMQRVGFDAFETRPDKDINEALKGLSDFSVRYQVSAD
                     EAQPLFRREQRGEAA"
     gene            1376..2077
                     /locus_tag="VFW59_06780"
     CDS             1376..2077
                     /locus_tag="VFW59_06780"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX5363955.1"
                     /translation="MSALKQKIEQTVEVLWQAAGEFAPVTFANSLGAEDMVLTDLIAK
                     RAIDIEMFSLDTGRLPAETYVLMQQLRERYKLPLKVYFPESASVEAYVAQHGVNGFYD
                     SVENRKGCCFVRKVEPLRRALSGKGAWVTGMRREQAATRGSLEVSSFDADNGLQKFNP
                     LLEWSNAEVWEYIKANDVPYNKLHDQFYPSIGCAPCTRAITPGEDIRAGRWWWENPEN
                     KECGLHVASSIPKRA"
     gene            2244..>3083
                     /gene="cysD"
                     /locus_tag="VFW59_06785"
     CDS             2244..>3083
                     /gene="cysD"
                     /locus_tag="VFW59_06785"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX5363956.1"
                     /translation="MSKHTFTHLDALESESIHIMREVAAECKNPALLFSGGKDSIVML
                     RLAEKAFRPCKFPFPLVHIDTEHNFPEVIATRDKLAADLGERLIVRSLEDSIKRGTIV
                     IKDESKPRNPYQSVTLLETIEEFGFDACMGGARRDEEKARAKERIFSHRDSFGQWQPK
                     AQRPELWTLFNTRLQPGEHFRVFPISNWTELDVWQYIEREQIALPSLYYAHQRRIVER
                     KGLLVPVTELTPAREGEQVIEKTVRFRTVGDITCTCPVDSPATTAAEIVIETLAADVS
                     ERGA"
BASE COUNT          648 a          869 c         1021 g          545 t
ORIGIN      
        1 gtcagatggc agcgaggcgc tggaagcgct ggcgagcgag gagaaggcct tcgcgaactg
       61 gctgaagcgc aacgtgaagc cgcacaagtt gcccggctac gcggtcgtcg tgctgtcgct
      121 gaagaagccg ggcgaggcgc ccggtgacgt gagcgccgag cagttcgatg cactggccga
      181 cctggccgac cgctacagct tcggcgaact gcgcgtgaca cacatgcaga atctggtgct
      241 ggccgacgtg aagcaatccg atctggtcga gctctggtac gcagcctccg agcagggatt
      301 ggcgacgccg aacatcggtt tgctgaccga cgtgatctgc tgcccgggga gcgatttctg
      361 caatctggca aatgcgcgtt ctatcccgct ggccacggcg gtctcgaacc gtttcgaaag
      421 catcgatttc cagcacgaca tcggcgacat cgagctgaac atctcgggct gcatcaatgc
      481 ctgcggtcac catcatgtcg gccatatcgg catactgggc gtcgacaaga acggcgagga
      541 tcgctatcag gtgtcgatcg gcggccagca gggcaacggt ctgcgtctgg ggaaggtgat
      601 cggcagttcg ttctccgcag cggaaacgcc ggaggtgatc gccagtctgc tggatctgta
      661 tgtgcaggag cggcacgagg acgagcgttt cgtcgatact gtgcagcgtc tgggcgtgca
      721 gccgttcaag gagcatgtgt acaagacggc gacagcggtc gcgctggggc tggcggacga
      781 tgcggcgttg catgaggaag ccgtcgtggc gagctacgac gatccttact attcgcggag
      841 gttttaaggt catgatcatc atgagagaga gcatcatccg taacggcagc atcgcgcagg
      901 atgactgggg cgtgttgcgg ctggatgaag gcgatacacc ggaaggcgta gaagtgccgc
      961 aagaaaagat catcgtgccg ctgaaggtat ggcaggcgca gcgcgagaag ctgcagcaca
     1021 ccgatctggg cgtgtggctg gacagcgacg agcgcgccga ggagctggcg ggcgatctcg
     1081 atcgctttgc ggtgatcgcg gtgaacttcc ccaagttcgc cgacggacgc ggctacaccc
     1141 tggcctaccg gttgcgcgtg cggctgggct atcgcggcga actgcgcgcg atcggcgacg
     1201 tgctgcgcga tcagctgttc tacatgcagc gggtcgggtt cgatgcgttc gagacgcgtc
     1261 cggacaagga catcaacgag gcgctgaagg ggctatccga tttctcggtg cgttatcagg
     1321 tctcggcaga cgaggcgcaa ccattgttca gacgcgagca gcgaggagaa gcagcatgag
     1381 tgcattgaaa cagaagatcg agcagaccgt ggaagtgctg tggcaagcgg ctggagaatt
     1441 tgctcccgtg acctttgcga acagtctggg tgcggaagac atggtgctga ccgatctgat
     1501 tgcgaagcgc gcgatcgata tcgagatgtt cagtctggac acgggacgtc tgcctgccga
     1561 gacctacgtg ctgatgcagc agttgcgcga acgttacaag ctgccattga aggtctactt
     1621 cccggaaagc gcgtctgtcg aagcctatgt cgcacagcat ggcgtgaacg gcttctacga
     1681 cagcgtcgag aaccgcaagg gctgctgctt cgtgcgcaag gtcgagccgc tgcgccgtgc
     1741 gctgtcaggc aagggcgcat gggtcaccgg catgcgccgc gaacaggcgg cgacgcgtgg
     1801 cagtctggaa gtgtcgtcgt ttgatgccga caacggcctg cagaaattca atccgctgct
     1861 ggaatggagc aacgccgagg tgtgggaata catcaaggcg aacgatgtgc cgtacaacaa
     1921 gctgcacgac cagttctatc cgagcatcgg ctgcgcaccc tgcacccgtg cgatcacacc
     1981 gggcgaagac atccgcgccg gaagatggtg gtgggaaaac ccggagaaca aggaatgcgg
     2041 cttgcatgtg gcaagcagca ttccgaaacg ggcgtagccc gtttgttctc cgggtttcgg
     2101 cgaagactta tgtcgcgcac gaagtaagcg acgttagtcg aagccaaaat ccggatggca
     2161 aagaatgcgg actgcatttt ggcaagagca agtagggtgg gttgagcgta gcgatacccg
     2221 ccatcaaatt gagagagaag ataatgagca aacacacctt tacccatctg gacgcactgg
     2281 aaagcgaatc catccacatc atgcgcgagg tcgcggcgga gtgcaaaaat cccgcgctgc
     2341 tgttctccgg cggcaaggat tccatcgtga tgctgcgcct cgcggagaag gcgttccgcc
     2401 cgtgcaagtt cccgttcccg ctggttcata tcgacaccga gcacaacttc ccggaagtga
     2461 tcgccacgcg cgacaagctg gcggcggact tgggcgagcg tctgatcgtg cgttcgctgg
     2521 aggactccat caagcgcggc accatcgtca tcaaggatga gagcaagccg cgcaacccct
     2581 accagtctgt gaccttgctg gagaccatcg aggagttcgg cttcgatgcc tgcatgggcg
     2641 gtgcgcgacg cgacgaggaa aaggcgcgcg ccaaggaacg catcttttcg caccgtgaca
     2701 gcttcggcca gtggcagccc aaagcccagc ggcccgagct ttggacgcta ttcaacaccc
     2761 ggctgcagcc cggcgagcat ttccgcgtgt tcccgatttc gaactggact gaactggacg
     2821 tgtggcagta catcgagcgc gagcagatcg cgctgccgtc gctgtattac gcgcaccagc
     2881 gcaggatcgt cgaacgcaag ggcctcttgg tgccggtcac cgaactgacg ccggcccgcg
     2941 aaggcgagca ggtcattgaa aagaccgtgc gctttcgcac cgtgggcgac atcacctgca
     3001 cctgccccgt cgacagcccg gccaccacgg ccgccgaaat cgtgattgaa acgctggccg
     3061 ctgacgtgag cgaacgcggc gcc
//
LOCUS       DASUIV010000185         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_787281, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000185 DASUIV010000000
VERSION     DASUIV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787281"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VFW59_06790"
     CDS             <1..975
                     /locus_tag="VFW59_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5363957.1"
                     /translation="PYLRLMKPMIVVEGCLKCHGYQGYKVGDVRGGVGVAIPMQPYLN
                     DLNASFFPRLVTLGLIWLLGVVAIVALIRQVQYSFDAQARAAADMERQNQIIARANAD
                     LQRFAEVTAHHLQEPARRISNYAERLTQQLGDRLDNAEARLSLEFIGQQARRQKNLLR
                     DIERYLAADQPRGKLESIDVQHLVAEMLRRHEPRISEAGATITVGDLPPSRFDSPRMT
                     DLFEVLLDNALIHGQSEHPLHIAIEGERLGSAVRYRVRDNGPGIEAQYREQIFRLFER
                     LTSSGEGTGIGLAIVRRITESCGGRAWIEENPGGGCCVLFELPAGEKP"
     gene            972..1418
                     /locus_tag="VFW59_06795"
     CDS             972..1418
                     /locus_tag="VFW59_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5363958.1"
                     /translation="MNPEKKRFVILLVEDELADAHLVKVALSENHILADLHHVSDGSE
                     ALEYLHRQGPRFADAPRPDLILLDLNMPRMDGRGFLSAIKQEADLRVIPVVVLTTSEV
                     ERDVVSSYDLGAAGYVTKPVDVVQFMAAIRCLSDYWIELVRIPQQR"
     gene            complement(1584..1841)
                     /locus_tag="VFW59_06800"
     CDS             complement(1584..1841)
                     /locus_tag="VFW59_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363959.1"
                     /translation="MFALPSLWNLLISTIVFFIAAWYIHRYLDEQGIPKGMTRGLLVF
                     VLASVVSWGAGEAVDWLQGAQPGEPAGDLQQRLKAVGQEQQ"
     gene            complement(1960..>2448)
                     /locus_tag="VFW59_06805"
     CDS             complement(1960..>2448)
                     /locus_tag="VFW59_06805"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5363960.1"
                     /translation="VIKLLNGYFTEMTAAIHQHGGTVDKFIGDGIMAFFGAPQLLECP
                     ERNAFEAAQEMLVRLEKYNLQLQALNQEKIRIGIGLHVGEVIVGHVGSESRNEYTAIG
                     DVVNTSSRLESITKSLGYPIICSAEVALAVRDHCDLVDLGEQPIKGRSAVRVYGYTPN
                     HA"
BASE COUNT          500 a          736 c          715 g          497 t
ORIGIN      
        1 ccttatctgc gcctgatgaa accgatgata gtcgttgaag gttgcctcaa gtgccatggc
       61 tatcagggtt acaaggttgg ggatgtgcgc ggcggagtcg gggtggctat ccccatgcag
      121 ccatacctga acgacttgaa cgcaagtttt ttcccccgtc tggtgacgct gggcctgatc
      181 tggctgctgg gtgtggtggc catcgttgcc ctgatccggc aggtgcagta cagcttcgac
      241 gctcaggctc gcgctgccgc cgacatggaa cgacagaatc agatcatcgc acgggccaat
      301 gccgatctgc agcgtttcgc cgaggtgacc gcgcaccatt tgcaggaacc cgcgcggcgc
      361 atatccaatt acgccgagcg cctgacgcaa cagctgggcg accggcttga caatgcggaa
      421 gccagactat cgctcgagtt catcggccag caggcacgcc gccagaagaa tctgctgcgc
      481 gatatcgaac gctatctggc ggcagatcag ccgcgtggaa aactcgaatc gatcgatgtg
      541 cagcatcttg tcgccgagat gctgaggcgc catgaaccca ggatcagcga agcgggcgcg
      601 accatcacgg tgggcgatct gccgccctcc cgcttcgact cgccgcgcat gaccgacctg
      661 ttcgaggtgc tgctggacaa tgccctgatc catgggcaga gcgaacatcc cttgcatatc
      721 gctatcgaag gcgaacgtct gggttcggcg gtgcgttacc gcgtccgcga caatggtccg
      781 ggtatcgaag cgcagtatcg ggagcagata ttccgcctgt tcgaacgctt gacatcgagt
      841 ggcgaaggga caggcatcgg attggccatc gtacggcgca tcaccgagag ttgcggcggg
      901 cgcgcgtgga tcgaagaaaa cccgggcggc ggatgctgcg tcttgtttga acttcctgca
      961 ggagaaaaac catgaatcca gaaaaaaaac gcttcgtcat cctgctggtc gaagatgagc
     1021 tggccgacgc tcatctggtc aaggtcgcgc tgtcagagaa tcacattctg gccgacctgc
     1081 atcatgtctc cgatggcagc gaggcgctcg aatacctgca ccgccaaggc ccgcgctttg
     1141 ccgacgcgcc gcgtccggat ctgatcctgc tcgatctcaa catgccccgt atggatgggc
     1201 ggggattcct ctccgccatc aaacaggagg cggatctgcg cgtgattccg gtggtggtgt
     1261 tgaccacctc ggaggtcgaa cgcgacgtgg tctcctccta tgatctgggc gctgccggct
     1321 atgtcaccaa gcccgtggat gtggttcagt tcatggccgc cattcgctgt ctgagcgact
     1381 actggatcga actcgtacgc atcccccaac agaggtaaag cgcccctgcc ccgaagttcg
     1441 ggacaatctc tcagagtaac gttgcaacga tacgctgact ggccaagccg taggggttgc
     1501 tgcgttgcgc gaacgactgg tagcaaccgt ttttcatttc aggcatcaag ccacccggcc
     1561 atcgacgggg aggctccgtg gctttactgt tgctcctgcc cgaccgcctt cagtcgctgt
     1621 tgcaggtcac ccgccggctc cccgggttgc gccccctgca gccagtccac ggcctcgcct
     1681 gccccccatg acacgacaga ggccagcacg aacaccagca agccgcgcgt catgcccttc
     1741 ggaatcccct gttcatccag atagcgatgg atgtaccagg cggcgataaa gaatacgatg
     1801 gtagagatga gcaggttcca cagggagggc agcgcgaaca ttgtcggttt cttgtgagtt
     1861 gagggagttc agtatgaggc agattttacc cgttcaccgt aatgaaccgg gcactgcctc
     1921 gcccctctgc ggcgggccgg aggggcttca ggaaaccatt caggcgtgat tcggcgtata
     1981 tccgtaaacc cggacagccg accggccttt gatcggctgt tcgcccaggt ccaccaagtc
     2041 acagtgatcc cggacagcaa gagccacttc agccgaacag atgatgggat agcctaacga
     2101 cttggtaata ctctccagac gtgaagaggt attcaccacg tcgccgatgg cggtgtattc
     2161 gttgcgcgac tccgaaccga catgtcccac gatcacttcg ccgacatgca agccgatacc
     2221 gatacggatc ttctcctggt ttagcgcctg caattgcaag ttgtacttct ccagcctaac
     2281 cagcatttct tgcgcggctt cgaatgcatt ccgctccggg cattcgagca attgcggcgc
     2341 tccgaaaaaa gccatgatgc cgtcgccaat aaatttgtcc acggttcccc catgctggtg
     2401 tatcgcggcg gtcatctcgg tgaaatagcc gttcaacaac ttgatgac
//
LOCUS       DASUIV010000186         4430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_879886, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000186 DASUIV010000000
VERSION     DASUIV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879886"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFW59_06810"
     CDS             complement(<1..190)
                     /locus_tag="VFW59_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX5363961.1"
                     /translation="MPRIDTGKLSARMRRGEVFSVECPSRDILKHVTSRWGVLVLVAL
                     MEGTHRFSELRRKVGGVSE"
     gene            280..1131
                     /locus_tag="VFW59_06815"
     CDS             280..1131
                     /locus_tag="VFW59_06815"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5363962.1"
                     /translation="MIVVTGATGQLGRLVIQSLLKKVPASGIVAAVRNVGKAEDLAAL
                     GVQVRQADYNQPASWDGALQGADKVLLISSSEIGQRTKQHQAVIDAAKRAGVRLLAYT
                     SVLHADTSILGLADEHRETEAVIRSSGVPFVLLRNGWYTENYAAGIPTALSLGAVYGC
                     AGNGRISSAARADYAEAAAVVLTSGNQTGKTYELAGDSAYTLSEFAAEISRQAGRAIS
                     YVNFPEAEYKKALLGAGLPELFAELLANSDTGVSRGALFDEGHQLGKLIGRPTTPLAT
                     IVKAALS"
     gene            complement(1187..2089)
                     /locus_tag="VFW59_06820"
     CDS             complement(1187..2089)
                     /locus_tag="VFW59_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5363963.1"
                     /translation="MERTMVNGGTYFLVTIAVFFWGANFLLGGYVIHDIPPLWAAAMR
                     FVLGALLMFAIVGVKRENLFAPLRKNIGIYFMLGVVGIVGFNLFFFYALGYTSANNAA
                     LIMATNPLLTTLLAAVVLGERPGLRHLVALPVALVGVAVVVTHGDLHSLRTLQLSIGD
                     MLMLAGDIFWALYNVLCRRYMPQASPLVNTTWIMTAGALVLLCVALGSDEQLATLGTR
                     AAVSMAVMTVGGTVLAYLFWGIGIARLGAAHTSIFMNLIPVFAVLLGALFEEMPTAPQ
                     LVGGLLVLGGVTISMLPSRRPAAA"
     gene            complement(2148..>4430)
                     /locus_tag="VFW59_06825"
     CDS             complement(2148..>4430)
                     /locus_tag="VFW59_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5363964.1"
                     /translation="NRKKTDRHCALRRRIARLAVGIAILFLCLPGVSRAAPVPDGWRA
                     EVVGVRGAAENDPSQDYRDAQHLQTGQPAVATAREENAAPKNSGTQHPEFARHDEAGI
                     KQRQLDQMSLRNERMWFMLGGSVLLGMTALFLLRLRRSHRLLEVSNEQLRQSQDKIGA
                     LNARLEQSVLSSSGELRQQTRYLHTLFETLPVTVWLKDTESRYLAINAPIKDRTPEEV
                     IGKTDLELWPDDIGAVLRASDLEVMQMRRRSTSEVSIRAQDGRIFWREIDKAPVIDED
                     GSVLGTVGVSRDITERRRLEVELANREREFRTLAENAPDNIVRYDLEGRIVYLNALLE
                     QTLGVRAADIIGKLDCEIHPDGSFDAYIKELNRVLASGEDGEVELEFTGGDNGKHYHH
                     VRMVAERDEGGRLTGVLSIGRDITGLKRYEEAREAALAEATRLANSRSEFLAHMSHEL
                     RTPLNGILGYTQILQRDKTLGERGADALNVIRHSGEHLLSLIEDILDLARIEAGHGEL
                     ATSNFALNPLLSIVAEIVGVLAREKELEFSCDLAADLPQGIRVDVKRLRQVLLNLLVN
                     AVKFTDTGRVVLRVSRVTASRLAFAIEDTGCGIAASELETIFQPFEQSGEGRQRFGGT
                     GLGLSISRQLVRLMGGDIAVASRIGEGSTFRFELELPAVDIAPLSLTAVPDAKASTET
                     QGDDSAGALILPPDEEIQTLYRLAQLGSMRDILLYAERIGLLDPRYRPFAARLQQMAE
                     NYQSQAILAYAERILHHGPG"
BASE COUNT          897 a         1428 c         1297 g          808 t
ORIGIN      
        1 tttcgctcac cccgcccacc ttgcggcgca gttcgctgaa acggtgcgtc ccctccatca
       61 aagccaccag caccagtacc ccccagcggc tggttacatg cttgaggatg tcgcgtgaag
      121 ggcattcaac agaaaagacc tcaccgcgcc gcattctggc cgacagtttt ccggtatcga
      181 ttcgtggcat gtcacttacc tttttgtgcg tacttacaaa aagtcattgt aatggatatt
      241 cttcgttcac cccatccacc cacgaagaaa ggaaacatca tgatcgtcgt caccggggcc
      301 accggacaac tgggccgcct cgtcatccaa tcgctgctga aaaaagtccc cgcttccggc
      361 atcgtcgccg ccgtacgcaa tgtgggaaag gccgaagacc tcgccgcgct gggcgtgcag
      421 gtgcgccagg ccgattacaa ccagcccgca agctgggatg gcgcattgca aggtgcggac
      481 aaggtgttgc tgatttcatc cagcgagatc ggccagcgta ctaaacaaca tcaggccgtc
      541 atcgatgccg ccaagcgggc tggtgtaaga ctgctggctt acaccagcgt gctgcatgcg
      601 gacacctcca tacttgggct ggctgacgaa cacagggaaa ccgaggcggt catccgcagc
      661 tccggcgtgc cgttcgtgtt gctgcgcaac ggctggtaca ccgagaacta tgccgcaggc
      721 atccccactg ccctctcgct gggtgcggta tacggctgtg cgggcaacgg acgcatctcc
      781 tccgccgcgc gtgccgacta tgccgaagct gccgccgtgg tgctgacctc cggcaaccag
      841 acaggcaaga cctacgaact ggcgggcgac agcgcctaca ccctgagcga attcgccgcc
      901 gaaatctcgc gccaagctgg ccgcgcgatc agctacgtaa attttcctga agcggaatac
      961 aagaaggcgt tacttggcgc gggcctgccg gaattgttcg ccgaattgct ggcaaactcc
     1021 gataccggcg tatccagggg cgcgctgttc gatgaagggc accaactggg caagctcatc
     1081 ggtcgcccca ccacgccgtt ggcgaccata gtcaaagcgg cgttgtcatg aataccagac
     1141 aggcctgatt ggcatcggct cattcccgcc tgaagataca catcactcac gccgctgccg
     1201 gacgccgcga cggcagcatc gaaatcgtca ccccgcccag caccagcaag ccgcccacga
     1261 gttgcggggc ggtcggcatc tcttcgaaca gcgcaccgag cagcacggcg aataccggaa
     1321 tgaggttcat gaagatggag gtgtgcgccg cgcccagccg cgcgatgccg ataccccaga
     1381 acagataggc cagcacggtg ccgccgactg tcatcaccgc catcgacacg gcggccctgg
     1441 tgccgagcgt cgccagttgc tcgtcgctgc cgagtgccac gcacagcagc acgagcgcgc
     1501 ccgcagtcat gatccaggtg gtgttcacca gcggcgaggc ttgcggcatg tagcggcggc
     1561 acagcacgtt gtacaaggcc cagaaaatat ccccggccag catcagcata tcgccgatgg
     1621 aaagctgcag ggtgcgcagg ctatgcaaat cgccgtgcgt caccaccacc gccacgccga
     1681 ccagcgcgac gggcaaggcg acgaggtggc gcaggccggg acgttcgccc agcacgactg
     1741 cggccagcaa ggtggtcagc aaggggttgg tcgccatgat cagcgcggcg ttgttggccg
     1801 aggtgtagcc cagcgcgtag aagaaaaaca gattgaagcc gacgatgccg accacgccca
     1861 gcatgaagta aatgccgata tttttgcgca gcggcgcgaa caggttttcg cgcttgacgc
     1921 ccacgatggc gaacatcagc agcgcgccca gcacgaagcg catggcggcg gcccacagcg
     1981 gcggaatgtc gtggatcaca taaccgccca gcagaaaatt cgcgccccag aaaaataccg
     2041 cgatggtcac caggaaatac gtgccgccgt tcaccatcgt tcgctccatt agaaaatttc
     2101 atcccgcaaa acagacaggc gctgctgaag ccgtgatgat agcggggtca gcccggccca
     2161 tgatgcaata tccgctctgc ataagcgagg atagcctgag actgatagtt ttccgccatc
     2221 tgctgcagtc gcgcggcgaa cggacgatag cgcgggtcga gcaggccgat gcgttcggca
     2281 tacagcaaga tgtcgcgcat gctgcccagt tgcgccagac ggtacagagt ctgtatttcc
     2341 tcgtccggcg gcaggatcaa tgccccggcg gaatcatcac cctgcgtctc ggtgctggcc
     2401 ttggcatcgg gcacagcggt caaggagagc ggcgcgatat ccacggcagg cagctccagt
     2461 tcgaaacgga aggtgctgcc ctcgccgata cggcttgcga ccgcgatgtc gccccccatc
     2521 aaccgcacca gttgacggct gatggagagt ccgagaccgg tgccgccgaa gcgctgccgc
     2581 ccctctccgg actgttcgaa gggctgaaag atcgtttcaa gctcgcttgc cgcgataccg
     2641 cagccggtgt cttcgatggc aaaggcaagc cgcgacgcgg tcacgcgact gacccgcaac
     2701 acgacccgcc ccgtatcggt gaacttgacg gcattgacca acagattcag cagcacctga
     2761 cgcaagcgct tgacgtcgac acggatgcct tgcggcaagt cggcagcaag atcgcagctg
     2821 aattcgagtt ctttctcccg cgccagcacg ccgacgattt cggcgacgat cgacaggagc
     2881 gggttcagcg caaaattgct cgtggcgagt tcgccgtgcc ccgcttcgat acgggcaagg
     2941 tcgagaatat cctcgatcag cgacagcaga tgctcgccgc tgtgacggat gacgttgagc
     3001 gcatcggccc cccgctcgcc cagcgtcttg tcgcgctgca atatctgcgt atagcccaga
     3061 attccgttca gcggcgtgcg cagctcgtgg ctcatgtggg ccaggaattc gctgcgcgaa
     3121 ttcgccaggc gcgtcgcctc ggccagcgcg gcctcgcgcg cctcctcgta gcgcttcaac
     3181 ccggtgatgt cgcgaccgat ggacagtaca ccggtcaggc gtcctccctc gtcacgctcg
     3241 gccaccatgc gcacatgatg gtagtgttta ccgttatcgc cgccggtaaa ttccagctcg
     3301 acttcgccat cctccccgct ggcgagcacg cgattaagtt cttttatata ggcatcgaaa
     3361 cttccatcgg gatggatttc gcaatcgagc ttgccaatga tgtccgcggc ccggacaccc
     3421 aatgtctgct caagcagggc attgagataa acgatacgcc cctccagatc gtaacgaacg
     3481 atgttgtccg gggcattttc cgccaaggtg cggaattcgc gctcgcgatt ggccaattcg
     3541 acctccagac gcctgcgctc ggtgatatcc cggctgactc cgaccgtacc cagcacgctc
     3601 ccgtcctcgt caatcaccgg agccttgtcg atttcccgcc agaagatgcg gccgtcctga
     3661 gcacggatgg atacttcgct ggtcgagcgg cggcgcatct gcatcacctc gaggtcgctg
     3721 gcgcgcagaa ctgccccgat atcgtccggc cacaactcca gatcggtttt tccgatcacc
     3781 tcttccggcg tgcgatcctt gatgggcgcg ttgatggcga ggtagcggct ctcggtgtct
     3841 ttcaaccaga cggttacggg cagcgtctca aacaaggtgt gcaaatagcg cgtctgctgc
     3901 cgcagttcgc cgctactgga caacacactt tgttcgaggc gggcattgag cgcaccgatc
     3961 ttgtcctgcg attgccgcag ttgctcattg gagacttcca gcagccggtg agagcgccgc
     4021 aaacgcagca gaaataatgc tgtcatgccc agcagcacgc tgccgcctag catgaaccac
     4081 atccgttcgt tgcggaggct catttgatcg agctgacgtt gcttgattcc ggcttcgtcg
     4141 tgccgcgcga actcgggatg ttgcgtgcct gaatttttcg gggcggcatt ctcctccctt
     4201 gcggtcgcaa cggcgggctg gccggtctgc aagtgctgcg catcccgata gtcctgagac
     4261 ggatcgtttt ccgccgcccc ccgcacacca acgacttcgg cccgccatcc gtccggcacc
     4321 ggggcggccc gggaaacgcc gggcagacac aggaagagta tcgcgatgcc aacggccagt
     4381 ctggcaatcc gcctacgcaa agcgcaatgc ctgtcagttt ttttacgatt
//
LOCUS       DASUIV010000187        14862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_416539, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000187 DASUIV010000000
VERSION     DASUIV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 14862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14862
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416539"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VFW59_06830"
     CDS             complement(<1..944)
                     /locus_tag="VFW59_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5363965.1"
                     /translation="MTHYAPLIGALTTLLVLTFILTVGKRAIIHDIPNERSLHTKPVP
                     RMGGIALLAGVLVGWLMLTPFWSWWIMLPTLGLFLLSLLDDIRNLSARTRLIVHFMAA
                     LIALSGANISLLWLIPLLLFIVWMTNLYNFMDGSDGLAGGMALFGFGSYGVGAWLSGN
                     EGFALLNFSIASAALSFLLFNFHPARIFMGDAGSIPLGFLAATLGIYGWQDGIWPLWF
                     PLLTFSPFIADATLTLYQRKCRGEKLSQAHRSHYYQRLVQMGWGHRNTALSEYALMLL
                     AGATALQARNATAENQIWLLLCWIISYGCLSMWIDHSWR"
     gene            1066..2052
                     /gene="waaC"
                     /locus_tag="VFW59_06835"
     CDS             1066..2052
                     /gene="waaC"
                     /locus_tag="VFW59_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX5363966.1"
                     /translation="MAPSFADTKTPQILLVKTSSLGDVLHNLPVVSDIVRHYPDARID
                     WVVEESFAALPRLHPAVRNIIPVAVRRWRRNLLKSDTWREIAAFRSTLATQSYDLAID
                     TQGLLKSAVLMRNARGLRCGFDSRSAREPLAANLYQQTFFVATGQHAVERNRQLVAQA
                     LDYSLEQPADYGIQPPMMARPGWLADESYAVLLHATSRDDKLWHEANWIALGHELHAR
                     NMRCVLPWGSEAERVRSQRLAASIPSAIVPPRLDLNEAADLLGGARAVVGVDTGLAHL
                     AAALGVPTVGIYTATDPVLTGLYAGRRLINLGNIAHAPSVAEVVASLQQVAW"
     gene            2049..3314
                     /gene="waaA"
                     /locus_tag="VFW59_06840"
     CDS             2049..3314
                     /gene="waaA"
                     /locus_tag="VFW59_06840"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEX5363967.1"
                     /translation="MSSRAFYSLALWLLLPYILLRLLWRARKQPEYLHHVGERFGFYP
                     VHDDRPVIWLHAVSVGETRATQSLVARLCAAYPDHRILLTHATPTGRAASEQLYGDQV
                     LRAYLPYDYPFAVSRFLRHFRPRLGILMETEIWPNLIHLAHANGTPLLLLNARLSEKS
                     ARGYARFAGLTRKALQQLAGIAAQTADDAARLAALGGRAVSVMGNLKFDIEAPPVMQE
                     LGRQLREQFGRERRVFLAASTREGEEALLLDVLLSKLPADCLLVLVPRHPQRFAEVAA
                     MLALRGIPYQRRSENRAVAPSVRVVLGDSMGEMFAYYAAADLAFIGGSLLPYGGQNLI
                     ESCAVGTPVLVGPYTYNFAEATRFALAAGAARQVRDVDGLVAETQSLLAQEDALFLMR
                     QGCVGFVASNRGATETALDIIQSRLSAKP"
     gene            complement(3327..4802)
                     /gene="ppx"
                     /locus_tag="VFW59_06845"
     CDS             complement(3327..4802)
                     /gene="ppx"
                     /locus_tag="VFW59_06845"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX5363968.1"
                     /translation="MRHQPILAAVDLGSNSFRLQIARVEGDQLYMLDGLREPVRLAAG
                     LTPDKYLDAEAQQRALDALGRFAERLRDLPPEAVRAVGTNSLRVAKNAEDFIPQAEHV
                     LGFPIEVIAGVEEARLIYLGVAHGLPPSNVNRLVIDIGGGSTEFIIGNGLAPLKLESL
                     YMGCISYSQRFFPDGVITKHNLKQAEIAARNELQTIASEYRGHWQKAFGSSGSAKVLA
                     EILELNGYSKSGITRTGLEHLRADLLKAGDVQLLELQGLRADRAPSLAGGFSIMYAAF
                     CELDIEQMQFAPTALREGVLYDLWGRFHHNDMRDVTTQQFMARYRADAGQAARVALLA
                     AELAQQFLDDGIGETALQTLDWAAKLHEIGISVAHSGYHKHTAYILKNADMPGFSKKE
                     QTRLSLLTLAQRGNLEKLQGQLENTEDLVLAMSLRLAVLFRRNRSDAPLPDMQGHFSG
                     TKFHLTIDTDWLTQNPLSETALQEESRQWKGLGVSLQVLAG"
     gene            complement(4802..5509)
                     /gene="phoU"
                     /locus_tag="VFW59_06850"
     CDS             complement(4802..5509)
                     /gene="phoU"
                     /locus_tag="VFW59_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX5363969.1"
                     /translation="MPSNDHISRQFDAELEAIRANVLQMGGLVESQIKSAVESLLSGD
                     VALMTRVIADDHRVNAMEVKIDEACSQVIARRQPTAGDLRLVMTVVKTITDLERIGDE
                     AEKIARMAKLLSQKHGLALPRYHEITHAAEIAVDMLRKSLDAFARLDVAMAAQVVRQD
                     DQVDEEFRAIMRYLITFMMEDPRTISTSLEILFVAKAIERIGDHAKNMSEYVIYMVKG
                     RDVRHVTVDEIEREVQK"
     gene            complement(5610..6269)
                     /gene="rpiA"
                     /locus_tag="VFW59_06855"
     CDS             complement(5610..6269)
                     /gene="rpiA"
                     /locus_tag="VFW59_06855"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX5363970.1"
                     /translation="MSQDDLKRAVAQAAIEYVPADCIVGVGTGSTANFFIDELAKIKN
                     KIRGAVASSEASAKRLQGHGIEVLSLNEAGDLPVYVDGADEITRHMHMIKGGGGALTR
                     EKIVAAASKKFICLCDSSKLVDVLGKFPLPIEVIPMARSSVARQLVALGGQPMLREGF
                     TTDNGNVILDVYNLKILNPVELESTLDHIAGVVTNGLFARRAADVLLLGTADGVKKMT
                     A"
     gene            6415..8046
                     /locus_tag="VFW59_06860"
     CDS             6415..8046
                     /locus_tag="VFW59_06860"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HEX5363971.1"
                     /translation="MQVRTVATTPFTDQKPGTSGLRKRVPVFKQPHYLENFVQSIFNT
                     IAAPAGATLVLGGDGRYYNREAIQTILKMAAANGFGRVLVGRGGILSTPAASCVIRKY
                     QTHGGIILSASHNPGGPDGDFGIKYNGNNGGPATETVTEAIYAASKTIAQYRLADAPD
                     VALDELGDSRIGGMTVSVIDPVDDYAELMESLFDFGAIRALLAGGFRIKFDAMHAVTG
                     PYAREILVGRLGAPADSVMNAVPLADFGNGHPDPNLTYAHELVEIVYGDNAPDFGAAS
                     DGDGDRNMILGKSFFVNPSDSLALIAANAGLAPGYKKGLAGIARSMPTSGAADRVAAA
                     LGIPCYETPTGWKFFGNLMDAGRVTLCGEESFGTGSDHVREKDGLWAVLFWLNILAVR
                     KQSVEAIVYEHWAKYGRNFYSRHDYEAIPSDAASGVMKLLKDSFESLQGAQFGHLQVK
                     LCDDFSYTDPVDGSVSNGQGVRILFVDGSRIVFRLSGTGTEGATLRIYLESFEPDVSR
                     HHLDAQEALSTLIGIALQISELRQRTGREAPTVIT"
     gene            8118..8504
                     /locus_tag="VFW59_06865"
     CDS             8118..8504
                     /locus_tag="VFW59_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5363972.1"
                     /translation="MANKTIIQTPDAPAAIGTYSQAVRVDHTVYLSGQIGLDPSSMQM
                     VEGIEAQIHRVFQNLRAVATAAGGSMDDVVKLNIFLIDLGNFAKVNEIMATYFHQPYP
                     ARAAVGVASLPRGALVEADGVMYLQD"
     gene            8567..10753
                     /gene="recG"
                     /locus_tag="VFW59_06870"
     CDS             8567..10753
                     /gene="recG"
                     /locus_tag="VFW59_06870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5363973.1"
                     /translation="MRESVRPKGASSRQAADNRFPVQMNSPAKIAPATLAKLAKLGIR
                     HRSDLLLHLPLRYEDETHLAPIATLQPGETVQVQGVITHSEVTFRPRRTLVCRLQDNG
                     SELYLRFMNFYPSQQKQLAVDKQIRAIGEVRMGYYGLEMVHPKCRAVGDDTPLQQSLT
                     PVYPTTAGLSQILLRKLIGNALEVLPLIDTLPESLLKRLRLASFADSLRLLHNPPPDV
                     AASSLEQRSHAAWQRIKFDELLAQQLSMRVHHRERSKRAAPALVPQHRLTDALLKQLP
                     FALTGAQQKVCAEIGRDLALPHPMQRLLLGDVGSGKTVVAALAALQAIENGYQVALMA
                     PTEILAEQHYLKLRDWLEPLGISPVWLSGSLKKKDKLLAAGRIASGETQLAIGTHALF
                     QSSVEFNRLGLAIIDEQHKFGVQQRLALRNKGVGEGAAEGRSEPHQLMMSATPIPRTL
                     AMSYYADLDVSVIDELPPGRTPVVTKLVSDARRDEVFERVRAACVTGQQAYWVCPLIE
                     ESEVSAQADVSAANVKRSGRSQSEALQLQSAVETYETLCTAFPELQVGLAHGKLDSAE
                     KARVMAAFKAGELQLLVATTVIEVGVDVPNASLMVIDHAERMGLAQLHQLRGRVGRGA
                     AHSLCILLYQQPLSELARARLKIIFESNDGFEIAQQDLRLRGPGELLGARQSGVAMLR
                     FADINEDEALLEQAQQAADELLRDHAEAARAHLQRWMASKHDYLHV"
     gene            10746..11291
                     /locus_tag="VFW59_06875"
     CDS             10746..11291
                     /locus_tag="VFW59_06875"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEX5363974.1"
                     /translation="MSDEYWHGTAVGCDEGLVPWLHDRGSLTRRIQQRCQHFAVRQMA
                     SGLARIAFDEAALLGIAPHQLAWSREVFLSADDNPVVFAHSACARQHLRGAWGAVRGL
                     GSKPLGALLFAHPLVERKPLHYKALHAHHPLHRKAATVLGVAPGRLWARRSLFYLHDA
                     PLLVTEVFLPDILRLGRAAGL"
     gene            11329..11949
                     /locus_tag="VFW59_06880"
     CDS             11329..11949
                     /locus_tag="VFW59_06880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5363975.1"
                     /translation="MQSKDHWEKVYTTKAVDAVSWFQEHADYSLALIRATGVAKDAAI
                     IDVGGGASTLVDDLLADGYRDLTVLDLSAVALESARKRLGAHAAGVDWIEADITQAEL
                     PARRFDVWHDRAVFHFLTSPEEREAYVQTVFRSVKPGGHVIVATFAEDGPTQCSGLPV
                     MRYRPDELHAEFGEAFSLLHHEKELHQTPSGAMQCFVYCYCRRVNS"
     gene            complement(11939..12577)
                     /locus_tag="VFW59_06885"
     CDS             complement(11939..12577)
                     /locus_tag="VFW59_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5363976.1"
                     /translation="MDKDKQTYRTTPMLRWSYSLIAPLYDFALSRPLGRVRAQSLESL
                     PTEEPGEVLLSGIGTGLDLPLLPDTHRYTALDFNAAMLSRAISQNNALQIDWVLGDSM
                     ALPFDDERFDHVVLHLILAVVPHPERCLSEAARVLKPGGTIVILDKFLRPQQQAWLRR
                     AANPLSRRLATRLDVVFEEVHRAAPELHKISDVPLLAGGWFRGIVLRKPAKN"
     gene            12664..13143
                     /locus_tag="VFW59_06890"
     CDS             12664..13143
                     /locus_tag="VFW59_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEX5363977.1"
                     /translation="MSKSNINIGIAEKDRKKIAEGLSRVLADTYTLYLKTHNFHWNVT
                     GPMFQTLHLMFMAQYNEIWLAVDIVAERIRALGYPAPGSYKEFSALTCIKDSNGVPNA
                     KEMIKQLVAGQEAVARTAREVLPIAEKAGDQPTVDLLSARMEVHEKNAWMLRSLLEE"
     gene            complement(13414..14484)
                     /locus_tag="VFW59_06895"
     CDS             complement(13414..14484)
                     /locus_tag="VFW59_06895"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HEX5363978.1"
                     /translation="MDNRLPDNCTTLYADLQQKVMDSIALEPGGSFVSKSVKGMTYWY
                     FQTPQVSGQRKQVFVGRETPELLARIETARLAKADAANILNERKRLVAMLSAGGATME
                     KGRPARIIDSMAKAGLFDGGGVLVGSFAFACYGNMLGVAFASSLSRTEDMDFSVQREI
                     QIGMSRDIGAELQRVEPSLKLPQQIRPSAKPFEMIASDGFKVEFLTTRDDPADKFPVP
                     IAQLSIHAQPLEYMDYLIENSQPAVLLYGAGVPVRVPDPARFALHKLAVSQLRPSGAK
                     NKADKDLRQAAAIIGVLLEDNPGLLLLAADAINERADLMCNIVRAGAALLPAPMKEEI
                     DNAVHGKEWDVGTGTAKVRRAK"
BASE COUNT         2708 a         4407 c         4736 g         3011 t
ORIGIN      
        1 cgccagctgt ggtctatcca catcgacagg catccatagg aaatgatcca gcacagcaac
       61 aaccagattt ggttttcggc ggtcgcatta cgtgcctgca atgcggtggc gccggccagc
      121 agcatcaatg catattcgct cagcgcggtg ttgcgatgcc cccagcccat ttgtaccagc
      181 cgttggtaat agtggctgcg gtgcgcttgc gagagttttt ctccgcgaca cttgcgctgg
      241 tagagcgtga gggtcgcatc ggcgatgaag ggcgagaagg tcagcagggg aaaccagagc
      301 ggccagatgc catcctgcca gccatatatg cccagggtag ccgcgaggaa gcccagcggt
      361 attgagcccg cgtcgcccat gaatattctg gcggggtgga agttgaagag cagaaaactc
      421 agggctgccg atgcgatgga aaagttcagc agggcgaaac cctcgttgcc ggataaccat
      481 gcgcctactc cataactgcc aaagccgaac agcgccatgc cgccggccaa tccgtccgag
      541 ccatccatga agttgtacag gttggtcatc cacacgatga acagcaggag cgggatgagc
      601 catagcagcg agatattggc gccggacagt gcgatcagcg cggccatgaa atggacgatc
      661 aggcgggtgc gggccgagag gttgcgtatg tcgtcaagca gcgacagcag gaacaggccc
      721 aaggtcggca gcatgatcca ccagctccag aagggcgtca gcataagcca gcccaccagc
      781 acgccagcca gcagcgcgat gccgcccatg cgcggcacgg gcttggtgtg cagcgagcgt
      841 tcgttgggga tgtcgtgaat gatggcgcgc ttgccgaccg tcaggatgaa tgtcagcacc
      901 agcagcgtcg tcagcgcacc gatcagcgga gcgtaatgcg tcatgccttt taccggaaat
      961 cgaggtggcg tattttacac taccgtccgc aaatcacgct gcgcatcttc ctaaggcgat
     1021 gccgcatcct ataagatgcg cgacagttca catcacggtt cccatatggc gccctctttc
     1081 gccgacacaa aaacgccaca gattctgctg gtcaagactt catcgctggg cgatgtgttg
     1141 cataacctgc ctgtcgtcag cgacatcgtg cgccattacc ccgatgcgcg catcgactgg
     1201 gtggtggaag aaagtttcgc cgccttgccc cgcttgcacc ccgccgtgcg caacatcatt
     1261 cccgtggcgg tgcgccgctg gcgccgcaat ctgctgaagt ccgacacctg gcgagaaatc
     1321 gcggcattcc gcagcacgct tgcgacgcaa agttacgact tggcgataga tacccaaggc
     1381 ctgctcaaga gcgctgtgct gatgcgaaat gcacgcggat tgcgttgcgg tttcgacagc
     1441 cgcagtgcgc gcgaaccgct ggcggccaat ctgtatcaac agacattttt cgtcgcgaca
     1501 gggcagcatg cggtcgagcg caatcgccag ctggtcgctc aggcgctgga ttactcgctg
     1561 gagcagcccg ccgattacgg cattcagccg ccgatgatgg cgcgtcccgg atggctggca
     1621 gacgagtctt atgccgtgct gctgcatgcc accagccgcg acgacaagct ctggcatgag
     1681 gcgaactgga tcgcgctggg ccatgagctg catgccagga acatgcgctg cgtgctgccc
     1741 tggggcagcg aggcggagcg ggtgcgcagt cagcgtctcg ccgcaagcat cccttccgcc
     1801 atcgtgccgc cgcggctgga tctgaacgag gccgctgacc tgctgggcgg tgcgcgggcg
     1861 gtagtcggtg tggatacggg actggcgcat ctggctgccg cattgggtgt gccaaccgtc
     1921 ggcatctaca ctgcgaccga tccggtgttg accggactct atgccgggcg acgcctgatc
     1981 aatctcggca atatcgctca cgcaccgagt gtcgccgagg tggttgcttc tctgcaacag
     2041 gtggcgtggt gagttcaagg gcgttttatt cgctggcgct gtggctgctg ctgccctata
     2101 tcctgctgcg cctgctatgg cgtgcgcgca agcagccgga atatctgcat catgtcgggg
     2161 agcgtttcgg attttatccc gtgcatgatg atcggccggt gatctggttg catgcggtct
     2221 cggtcggcga gacccgcgct acccagagtc tggttgcgcg cttgtgcgcc gcgtaccctg
     2281 atcaccggat attgcttacc cacgctacgc ccaccggacg cgccgccagt gagcagttgt
     2341 atggcgatca ggtgctgcgc gcctacctgc cctacgacta tccgtttgcg gtgagccgct
     2401 tcctgcgtca cttccgtccg cgactcggca tcctgatgga aacggagatc tggcccaacc
     2461 tgatccacct cgctcatgcg aatggcacgc cgttgttgtt gctgaatgcc cgcctgtcgg
     2521 aaaaatcggc gcgcggttac gcgcgattcg ccgggctgac gcgcaaggca ttgcagcaac
     2581 tcgccggcat cgccgcgcaa accgccgacg atgccgcacg cctggctgca ttgggcggca
     2641 gggcggtgtc ggtgatgggc aacctgaaat tcgatatcga ggctccgcct gtcatgcagg
     2701 aactggggcg gcagctgcgc gagcagttcg gtcgtgagcg cagggtattc ctggctgcca
     2761 gcacgcgcga gggggaggag gcgctgttgc tcgatgtgct gttgtcaaag ctgcctgcag
     2821 attgtttgct ggtgctcgtg ccgcgtcatc cgcagcgctt cgcggaggtt gcggccatgc
     2881 tggcgctgcg cggcatcccg tatcagcgcc gctccgagaa tcgcgccgtc gcaccatcgg
     2941 tgcgcgtggt gctgggcgac agtatgggcg agatgttcgc ctactatgcc gccgccgatc
     3001 tggcctttat cggcggcagt ctgctgcctt acggcggaca gaatctgatc gaatcctgcg
     3061 ccgtcggcac gcccgtgctg gtcggtccgt atacctataa tttcgccgag gcgacgcgct
     3121 ttgcgctggc ggcaggagcg gcgcgacagg ttcgggatgt tgacggattg gttgcggaga
     3181 cgcagagctt gttggcgcag gaagatgcgt tgtttctcat gcgtcagggt tgtgtcgggt
     3241 tcgtggcgtc caatcgcggt gcaacggaaa cggcactgga tatcatccag tccaggctat
     3301 ctgcaaagcc gtgatctgat cgcaggctag cccgccaata cctgcaagct cacgcccagt
     3361 cccttccatt gcctgctttc ttcctgcaag gcagtctcgc tcaacgggtt ttgagtcagc
     3421 caatccgtgt cgatggtgag gtggaacttg gttccgctga aatgaccttg catgtcgggt
     3481 agcggtgcgt cgctgcggtt gcggcggaac agcacggcca gacgcaggct catcgcgagc
     3541 acgaggtctt cggtgttttc cagctgcccc tgcagcttct ccagattgcc gcgttgtgcc
     3601 agtgtcagca ggctcagccg ggtttgctcc tttttcgaga agccgggcat gtcggcgttc
     3661 ttcaggatgt aggcggtatg cttgtgatag ccgctgtgcg cgacactgat gccgatctcg
     3721 tgcagtttgg ctgcccagtc cagtgtctgt agcgccgttt cgccgattcc atcgtcgagg
     3781 aactgctgcg ccaattcggc tgcgagcagc gctacacggg cagcctgtcc ggcatctgcc
     3841 cggtagcgtg ccatgaactg ctgtgtcgtg acgtcgcgca tgtcgttgtg gtggaagcgg
     3901 ccccacaggt cgtagagcac gccttcgcgc agggcggtgg gggcgaattg catctgttcg
     3961 atgtcgagct cacagaaggc ggcgtacatg atggaaaaac cgccagccag cgagggggcg
     4021 cgatcggctc gcaggccttg cagctccagc agttgcacat cgccagcctt gagcaggtcg
     4081 gcacgcaggt gttcgagtcc tgtgcgcgtg atgccgctct tgctgtagcc gttcagttcc
     4141 agtatctcgg ccagcacctt ggctgaaccc gaagagccga atgccttttg ccagtggcca
     4201 cgatattcgc tggcgatggt ctgcagttcg ttgcgcgccg cgatctcggc ctgtttgaga
     4261 ttgtgcttgg tgatgacgcc gtcgggaaag aaacgctgac tgtaactgat gcagcccatg
     4321 tacaggcttt ccagcttgag cggggcgagg ccattgccga tgatgaattc ggtggagccc
     4381 ccgccgatgt cgatcaccag ccggttgacg ttcgagggcg gcagtccgtg cgccacgccg
     4441 aggtagatca ggcgggcttc ttcgacgcca gcgatgactt cgatgggaaa ccccagcaca
     4501 tgctcggcct gcgggatgaa gtcttcggcg ttcttcgcta cgcgcagcga gttggtgccg
     4561 acggcgcgca ccgcctcagg gggcaggtcg cgcaggcgtt cggcgaagcg ccccagcgcg
     4621 tcgagcgcac gttgttgcgc ttcggcatcg agatatttgt cgggggtgag gccggcggca
     4681 aggcgtaccg gttcgcgtag cccatccagc atatagagct ggtcaccctc gacgcgtgcg
     4741 atctgcagcc gaaaactgtt cgatcccaga tcgacggcag cgaggatggg ctggtgccgc
     4801 actatttctg aacttcgcgt tcgatctcgt ccacggtcac atgacgtacg tcgcggcctt
     4861 tgaccatgta gatcacatat tcggacatgt tcttggcgtg gtcgccgatg cgttcgatgg
     4921 ccttcgccac gaacaggatt tccagcgagg tggagatggt gcgcgggtct tccatcatga
     4981 aggtgatcag gtaacgcatg atggcgcgga attcctcgtc cacctgatcg tcctgacgca
     5041 ccacctgagc ggccattgcc acatccagtc gcgcgaacgc atccagcgac ttgcgcaaca
     5101 tgtcgacggc gatttcggcg gcatgggtga tctcgtgata gcgcggcagc gcgagaccgt
     5161 gtttctgcga cagcagcttg gccatgcgtg cgatcttctc ggcctcgtcg ccgatgcgtt
     5221 ccagatcggt gatggtcttg accaccgtca tcaccagtcg caggtcgccc gcagtcggct
     5281 ggcggcgggc gatcacctgg ctgcatgcct cgtcgatctt cacctccatc gcattgacgc
     5341 gatggtcgtc ggcgatcacg cgcgtcatca gcgccacgtc gccggagagc agcgattcga
     5401 cggcgctctt gatctgactt tctaccagtc cgcccatctg caggacattg gcgcggatgg
     5461 cttccagctc ggcatcgaac tggcgggaga tgtggtcatt gcttggcatg ctggtattcc
     5521 ttaaaaatcg accggttagt gtaagcggct aatgtgaacg ttagatgaca gccgcgtcgc
     5581 cctctccggc agggaagggg caatggcatt taggcggtca ttttctttac gccgtccgct
     5641 gtgccgagca gcagtacgtc ggccgcacgg cgtgcgaaca ggccgttggt caccacgccg
     5701 gcgatgtgat ccagcgtcga ttccagttct acaggattca ggatcttcag gttgtacaca
     5761 tcgaggatca cattgccgtt gtcggtggta aatccctcgc gcagcatggg ctgaccgccc
     5821 agcgcgacca gttggcgtgc caccgaactg cgcgccatcg ggatgacctc gattggcagc
     5881 gggaatttgc ccagtacgtc taccagtttg ctggaatcgc acaggcagat gaatttcttg
     5941 ctggccgccg cgacgatttt ctcgcgcgtc agcgcgccgc cgccgccctt gatcatgtgc
     6001 atgtggcggg tgatctcgtc cgcgccgtcg acgtataccg gcaaatcccc ggcctcgttc
     6061 agcgacagaa cttcgatgcc atgaccctgt aaacgtttgg ctgaagcctc cgagctcgcg
     6121 actgcgccgc gtattttgtt cttgatcttg gccagttcgt cgataaaaaa gttggcggta
     6181 gagccggtgc ccacgccgac gatgcagtcg gcgggtacat attcaatggc agcttgggcc
     6241 accgcacgct tcaaatcgtc ttgactcatc atggcaagtt tctttctctt gtgtgtgtat
     6301 ttagggttgt gcccgcgatt attgcaggaa ttgcggtgca ggggaacagg cgtggtcgcg
     6361 cagatcgagt ggtaaaatgc acattcggga ttacagacaa cgggaggaac gaatatgcag
     6421 gttcgcacgg ttgcaacgac accctttacc gatcagaagc caggcacctc cggtttgcgc
     6481 aagcgcgtac cggttttcaa gcagccgcat tatctggaga acttcgttca atccatcttc
     6541 aataccatcg cagcacccgc cggcgcgacg ctggtgctcg gtggcgatgg gcgctattac
     6601 aaccgcgagg cgatccagac cattctgaaa atggctgccg ccaacggctt tggccgcgtg
     6661 ctggtcggtc gtggcggcat cctctctacg ccggctgctt cctgcgtgat ccgcaaatat
     6721 cagactcacg gcggcatcat cctgtcggcc agccacaatc ccggcggacc tgatggcgac
     6781 ttcggcatca agtacaacgg caacaacggt ggccccgcga ccgaaaccgt caccgaggcg
     6841 atctacgccg ccagcaagac catcgcccaa tatcgccttg ccgatgcgcc cgatgttgcg
     6901 ctcgacgaac tgggcgacag ccggatcggc ggtatgacgg tgtcggtgat cgatccggtg
     6961 gatgactatg cggaactgat ggagtcgctg ttcgatttcg gcgcgattcg cgcgctgctt
     7021 gccggcggct tccgcatcaa gttcgatgcc atgcatgccg tcaccgggcc ctatgcgcgc
     7081 gagatactgg tggggcgtct gggtgctccg gcggacagcg tgatgaatgc cgtgccgttg
     7141 gcggacttcg gcaacggcca tcccgacccc aatctgacct atgcgcacga actggtcgag
     7201 atcgtttacg gcgacaacgc accggatttc ggcgcggcct cggatggcga cggcgaccgc
     7261 aacatgattc tgggcaagag cttcttcgtt aacccttccg acagcctcgc gctgatcgcc
     7321 gccaatgccg ggcttgcccc gggctacaag aaagggttgg cgggtattgc gcgttcgatg
     7381 cctaccagcg gggcggcgga tcgcgttgct gccgcgcttg gcatcccctg ctatgagacg
     7441 ccgaccggat ggaagttctt cggcaacctg atggatgcgg gtcgcgtcac gctgtgcggg
     7501 gaagagagtt tcggcaccgg ctccgaccac gtgcgcgaga aggacgggtt gtgggcggtg
     7561 ctgttctggc tcaatatcct cgcggtgcgc aagcaatcgg tcgaggccat tgtgtacgag
     7621 cactgggcga aatacggccg caatttttat tcgcgccacg actacgaagc catcccgtcc
     7681 gacgctgcca gcggcgtgat gaagctgctg aaagacagct tcgaatcgct tcagggcgcg
     7741 cagttcggtc atttgcaggt caagctgtgc gatgacttca gctacaccga tccggtggac
     7801 ggcagtgtca gcaacggcca gggtgtccgt atcctgttcg tcgatggctc gcgcatcgtg
     7861 ttccgcctgt ccggcacggg caccgagggc gcgacgctgc gcatctatct ggagagtttc
     7921 gagccggatg tgagccgtca tcacctcgat gcgcaggagg ctttgtcgac gctgatcggc
     7981 attgcgctgc agatatccga attgcgccag cgcaccggac gcgaggcgcc gacagtcatc
     8041 acctgagccg ctataatcgc gcctcaaata cgcatgccgc gccggacagg cgcggtttta
     8101 tctttcaagg agcaatcatg gcaaacaaaa ccattatcca gacccccgat gccccggctg
     8161 caatcggcac ttattctcag gcggtgcgcg tcgatcacac cgtttacctg tccggccaga
     8221 tcggtctcga tccttcctcc atgcagatgg tcgagggcat cgaggcgcag attcatcgcg
     8281 tgttccagaa tctgcgcgcg gtcgcaaccg cagcgggcgg cagcatggac gacgtggtga
     8341 agctgaacat ctttctgatc gatctcggca atttcgccaa ggtgaacgaa atcatggcga
     8401 cctatttcca tcagccctac cctgcccgcg ccgccgtggg ggttgcctcg ctgccgcgcg
     8461 gcgcgctggt cgaggccgac ggcgtgatgt atctgcagga ttaaggcggt acgaatgaag
     8521 gctaacccgt ccgcagattt caccgattga acctcaaaca taaaatctgc gtgaatctgt
     8581 ccgcccaaag ggtgcaagtt cgaggcaagc tgcggataac aggtttccgg tgcagatgaa
     8641 ttctcccgca aagatcgcgc ccgcgacgct ggccaagctc gccaagctgg gcatccgtca
     8701 ccgttcagac ctgctgctgc acctgccgct gcgctacgag gacgagactc atctcgcgcc
     8761 gattgcgacg ctgcaacccg gcgagacggt tcaggtgcag ggcgtcatca cccatagcga
     8821 agtgacgttc cggccacgcc gtacgctggt gtgccgcttg caggataacg gtagcgagct
     8881 gtatctgcgc ttcatgaatt tctatcccag ccagcagaaa caactggcgg tggacaagca
     8941 gatccgcgcc atcggcgagg tgcgcatggg ctattacggg ctggagatgg tgcatccgaa
     9001 atgccgcgcg gtgggcgacg acacgccgct gcagcagagc cttaccccgg tttatccgac
     9061 tacggcgggg ttgtcgcaga tcctgttgcg caagctgata ggcaatgcgc tggaggtgtt
     9121 gccgttgatc gataccctgc cggagtcgct gctgaaaaga ctgcggctgg cgagtttcgc
     9181 cgatagcttg cggctgttgc ataacccgcc gccggacgtg gctgcgagca gtctggagca
     9241 gcgcagccat gcggcctggc aacgtatcaa gttcgacgag ttgctggcgc agcaattgtc
     9301 gatgcgggtg catcaccgcg agcgcagcaa gcgcgccgcc cccgccctcg tgccgcaaca
     9361 ccggcttact gatgcgctgc tgaagcagct gccgttcgcg ctgaccggcg cacagcaaaa
     9421 agtatgtgcc gagatcggtc gcgatctggc cttgccgcat ccgatgcagc gcctgctgct
     9481 cggcgatgtc ggcagcggca agactgtggt tgccgccctt gccgccctgc aggccatcga
     9541 gaacggctat caggtcgcgt tgatggcgcc gactgaaatc cttgctgagc agcattacct
     9601 caagctgcgc gactggctgg agccgctggg catctcgccg gtatggctgt ccggcagcct
     9661 gaagaaaaaa gacaagcttc tcgccgccgg gcgcatcgcc agcggcgaga cgcagctggc
     9721 catcggcacc catgcgctgt ttcagagttc ggtcgaattc aacaggctgg gcctggcgat
     9781 catcgacgag cagcacaagt tcggtgtgca gcaacgcctc gccttgcgca acaagggggt
     9841 tggggagggc gctgccgaag ggcgcagcga accgcatcag ctgatgatga gcgccacccc
     9901 cattccgcgc acgctggcga tgagttatta cgccgatctc gatgtgtcgg tgatcgacga
     9961 acttcccccg ggacgtacgc cggtggtcac caagctggtc agcgatgcgc gccgcgatga
    10021 ggtgttcgag cgcgtgcgtg cagcctgtgt gacggggcag caggcctact gggtgtgtcc
    10081 gctgatcgaa gaaagcgaag tttcggcgca ggctgacgtg agtgcggcga acgttaagcg
    10141 tagcggccgt agccaatctg aggccttgca actccagtcc gcagtcgaga cctacgagac
    10201 gttgtgcacg gctttcccgg aattacaagt cgggctggcg cacggcaagc tcgacagcgc
    10261 cgagaaggcg cgggtgatgg cagcgttcaa ggcgggcgag ctgcaattgc tggttgccac
    10321 caccgtcatc gaagtgggag tggatgtgcc aaacgccagc ctgatggtga tcgaccatgc
    10381 cgagcgcatg gggctggcgc aactgcacca gttgcgcgga cgggtggggc gcggtgcggc
    10441 gcacagcctg tgtatcctgc tttaccagca gccattgtcc gaactggcgc gggcaaggct
    10501 gaagatcatt ttcgagagca acgacggctt cgagattgcg caacaggatc tgcgattgcg
    10561 cggcccgggc gaattgctcg gtgcgcgtca gagcggggtg gcgatgttgc gctttgccga
    10621 catcaacgaa gacgaagcac tgctggagca ggcgcaacag gccgccgacg aattgttgcg
    10681 cgatcacgcg gaagcggcgc gcgcacattt gcagcgctgg atggcaagca aacatgacta
    10741 cctgcatgtc tgacgaatat tggcacggca ccgccgtggg ctgcgacgag ggcctggtgc
    10801 cctggttgca cgaccgtggc tcgctgacgc ggcgcatcca gcaacgctgc cagcattttg
    10861 cggtgcggca gatggcgagc gggctggcgc gcatcgcatt cgacgaggcg gcgctgctgg
    10921 gtatcgcgcc ccatcagctg gcatggtcgc gcgaggtgtt cctgtctgcc gacgataatc
    10981 ccgtggtgtt cgcgcacagc gcgtgcgccc ggcagcatct gcgcggcgca tggggtgcgg
    11041 tgcgcggtct cggcagcaag ccgctcggcg ccctgttgtt cgcccatccg ctggtcgaac
    11101 gcaagccgct gcactataag gcattgcatg cgcatcatcc cttgcaccgc aaggctgcca
    11161 cggtgcttgg ggttgcaccg ggacgtctgt gggcgcgccg ttcgttgttc tatttgcacg
    11221 atgcgccgtt gctggtgaca gaggtgttct taccggatat tctgcggctc ggtagggcgg
    11281 caggcttgta gcgggaattg acgacgcggc atttcatcag gagcagacat gcaatctaag
    11341 gatcattggg aaaaggtcta cacgaccaag gcggtcgatg cggtgagctg gtttcaggag
    11401 cacgccgatt attcgctggc actcatccgc gccactggcg ttgccaagga tgccgccatc
    11461 atcgacgtgg gtggcggcgc ctcgacgctg gtggatgatc tgctggccga cggttaccgt
    11521 gatctgacgg tgctcgatct gtctgctgtc gcgctggaat cggcacgcaa acgactgggt
    11581 gcgcatgccg ccggggtgga ctggatcgag gccgacatca ctcaggccga gctgcctgcc
    11641 cggcgtttcg atgtctggca tgatcgtgcc gtgttccact ttctcaccag tccggaagag
    11701 cgggaagcct acgtgcagac ggtgttccgt tcggtgaagc cgggagggca tgtcatcgtc
    11761 gccactttcg ccgaagacgg gccgacccag tgcagcggtc tgccggtgat gcgttaccgg
    11821 ccggacgaat tgcatgcgga attcggcgag gccttctcgc tgctgcatca cgaaaaggag
    11881 cttcatcaga ctccttccgg ggcgatgcag tgtttcgtct attgctattg ccgcagggtc
    11941 aattcttagc gggtttgcgc agcacgattc cgcgaaacca gccgccggcg agcagcggca
    12001 cgtcgctgat cttgtgcagt tccggtgcgg cccgatgcac ttcctcgaac accacatcca
    12061 gccgggtggc cagccggcgc gacagcggat tggccgcgcg ccggagccaa gcttgctgct
    12121 gcggacgcag gaacttgtcg aggatgacga tggtgccgcc cggtttcagc acgcgcgcag
    12181 cctcggacag gcagcgttcc ggatgcggca cgacggcgag gatcaggtgc agcacgacat
    12241 gatcgaagcg ctcgtcgtcg aacggcagcg ccatgctgtc gcccagcacc cagtcgattt
    12301 gcaatgcgtt gttctgggag atcgcgcggc tcagcatcgc ggcattgaaa tcgagcgccg
    12361 tatagcgatg ggtgtccggt aacagcggca agtccagacc ggtgccgatc ccgctcaaca
    12421 agacctcgcc cggctcttcc gtcggcagcg attccagcga ctgtgcgcgt acccggccca
    12481 gcgggcgcga gagggcaaag tcgtagagcg gcgcgatcag gctgtagctc cagcggagca
    12541 tgggcgtggt gcgataggtt tgtttatctt tatccatgcc gcgagtgtag tggaaatctg
    12601 gctgaactgg gtatacagtg cctcctcggt tctgcatccc aacctgcaat aaaggagggc
    12661 atcatgagca agtcgaatat caacatcggc atcgccgaaa aagaccgcaa gaagatcgcc
    12721 gaaggcctgt cgcgcgtact ggccgatacc tacaccctgt atctcaagac ccacaacttc
    12781 cactggaacg tcaccgggcc gatgttccag accctgcatc tgatgttcat ggctcaatac
    12841 aacgaaatct ggctggcggt cgacatcgtc gcggagcgta tccgggcact gggttacccg
    12901 gcccccggca gttacaagga attttccgca ctgacctgta tcaaggatag caacggcgtg
    12961 cccaatgcga aggagatgat caaacaactc gtcgcggggc aggaggcggt agcgcgtacc
    13021 gcgcgcgaag tgttgcccat cgccgagaag gcgggagacc agcccacggt cgacctgctc
    13081 tccgcccgca tggaagtgca tgaaaagaac gcctggatgc tgcggagctt gttggaagaa
    13141 taagcgcgtt gcgctaacgg tgctggcgtt ctttcggcgt aggctcatgc ggcgtttttt
    13201 gccgcgtgat gccgaagcca aaaaaatgcg tggatgttac gaggctcgtc ggaatagtag
    13261 aggcatcgaa ttgaataggc cacggtaaaa tcgtggccta ttcaattcga tggtgaccct
    13321 cgctgtcccg gcgccccgca gtctccccgc ctcacttcct tgcgtcaata gagccgaata
    13381 tctatatatt ccagccctgt gttcctgtgc agcttatttc gctctccgca cctttgccgt
    13441 ccctgtaccg acatcccatt ccttgccatg cacggcattg tcgatttctt ccttcattgg
    13501 ggccggaagc agtgcggccc cggcgcgcac gatgttgcac atgaggtctg cgcgttcgtt
    13561 gatcgcgtcg gcggccagca gaagcaggcc cggattgtct tcgagcagga cgccaatgat
    13621 cgccgcagcc tgacgcagat ccttgtccgc cttgttcttc gcacccgagg gacgcagttg
    13681 tgataccgcc agtttgtgca gggcgaatct ggccgggtcg ggcacgcgca ctggcacgcc
    13741 cgcgccgtac aggaggacgg cgggctggct gttttcgatc agatagtcca tgtattcgag
    13801 cggctgggcg tggatggata gctgcgctat cggcaccggg aacttgtcgg cgggatcgtc
    13861 tctggtggtc aggaattcga ccttgaatcc gtcgctcgcg atcatctcga agggttttgc
    13921 cgacggcctg atctgctgcg gcaatttgag gctgggttcc acgcgctgca actctgcgcc
    13981 gatgtcgcgc gacatgccga tctgtatttc ccgctggacg gagaaatcca tgtcctcggt
    14041 gcgcgacagg ctggaggcga atgcgacgcc cagcatgttg ccgtagcagg cgaacgcgaa
    14101 cgagccgacc agcacgccgc cgccgtcgaa caatcccgct ttcgccatgc tgtctatgat
    14161 ccgggcgggc cgccctttct ccatcgtcgc gccgccggcg gagagcatgg ccaccagccg
    14221 cttgcgctcg ttcaggatat tggcggcatc ggccttggcg agtctcgccg tttcgattct
    14281 ggcgagaagt tcgggcgttt ccctgccgac gaatacctgc ttgcgctggc cggagacctg
    14341 cggcgtctgg aagtaccagt aggtcatgcc cttgaccgac ttggatacga acgaaccgcc
    14401 cggttcgagc gcgatgctgt ccatgacctt ctgctggaga tcggcataca gggtggtgca
    14461 gttatcgggg aggcggttgt ccatggttat actcagatta atttgaagtg agtataacca
    14521 tattcgctgg gcgaaatggg cgcaatcgca aattctgtgc ggagatgccg ttttcccgtc
    14581 gcgatgtggt ggtcgagatc gtcgccggat agttgttcag ctcgatgagg ctgctcgcag
    14641 atggctggca catgaattcg ctatcgcgca gcgtgtgatg ccggcgccta gaacaccagc
    14701 tcttcgcctt cccatgccag acgcacatcc agtccgttta gtgcggtcgc gtatctggct
    14761 agtgcggcgg cgaaggctcg ctccaggtcg tcgtccgagc gcgtcggttc atggtgggtg
    14821 cagtacagga tttttgcgcc cgccgcgatc gccagctcga tg
//
LOCUS       DASUIV010000188         7249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_787672, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000188 DASUIV010000000
VERSION     DASUIV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787672"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFW59_06900"
     CDS             complement(<1..280)
                     /locus_tag="VFW59_06900"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5363979.1"
                     /translation="MSRLISFAQADSSPGYDHPKAERLVAGNPLRTTREHYVNSSGEV
                     SCGVWACEVGSWRIEFDERSDEYFYVLEGRICITGADGKACEFGPGDAC"
     gene            complement(280..1146)
                     /locus_tag="VFW59_06905"
     CDS             complement(280..1146)
                     /locus_tag="VFW59_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5363980.1"
                     /translation="MPVAGLLSGALVWGLIWYPFRVLEFAGVGGALTTLLTYSLAMVS
                     GAFMLPRVWRELPAAGWWALLLVLSSGWTNFGYMLAILDGEVMRVLLLFYLAPVWTIL
                     FSFWLLGERLNRYGYAIILLSLAGAFVMLWEPQLGLPWPNSLAEWVGLSAGMGFALSN
                     VVSRRAAHLSVESKSFSVWLGSALLSGLFLLAQGALLERVALIDGPSWLMIGLLGIVL
                     CSSSFAVQYGIARVPANRAIILFLFELVVAAVASYFLAGEAMPLRDWLGAALIVSASL
                     LSGKLYSELKES"
     gene            1232..2422
                     /gene="dapC"
                     /locus_tag="VFW59_06910"
     CDS             1232..2422
                     /gene="dapC"
                     /locus_tag="VFW59_06910"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX5363981.1"
                     /translation="MNPDLQRLQPYPFQKLAKLFGEVKPNPGYSAISLSIGEPKHATP
                     QFIKEALGAGLDGLANYPTTAGSDALRGTISEWLGKRYSIPALDAKTQILPVNGSREA
                     LFSFAQAVIDRSQPDPVVVSPNPFYQIYEGAALLGGATPHFLNTLPEDDYALNFAQLP
                     EAVWQRTQLIYVCSPGNPNGHVMPLSEWQTLFEMSDRYGFVIASDECYSEIYFDTPPL
                     GALQAAQQLGRCDYRNLVVFSSLSKRSNVPGMRSGFVAGDASIIAKFTLYRTYHGCAM
                     NPAIQSASIAAWNDEAHVAENRRLYAEKFARVVEILKPVLPVTLPDASFYLWIRTPIA
                     DTAFAQALYRDYNVTVLPGSYLAREARGINPGENFVRLALVANTEQTVEAAQRIAKFV
                     STLN"
     gene            2443..3264
                     /gene="dapD"
                     /locus_tag="VFW59_06915"
     CDS             2443..3264
                     /gene="dapD"
                     /locus_tag="VFW59_06915"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX5363982.1"
                     /translation="MDQLQQIIETAFERRAEITPRNADAQLKEAVDHVITQLDQGKLR
                     VAEKIDGDWVTHQWLKKAVLLSFRLEDNAFIKGGFTNYYDKVPSKFADYNSRDFREGG
                     FRVVPPAAARKGAYIAKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPVQANPTIIGDNCFVGARSEIVEGVILEDNVVISMGVFIGQSTKIYDRE
                     TGEVTYGRVPAGSVVVSGSLPSKDGSYSLYCAVIVKKVDAKTLSKVGINELLRGI"
     gene            3357..4526
                     /locus_tag="VFW59_06920"
     CDS             3357..4526
                     /locus_tag="VFW59_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5363983.1"
                     /translation="MIITPLLQLAVDKQASDLFFSVGAPVNIKIDGIAMPVNAQLLDA
                     DVIKRIAYEMMSPKQAAEFEAKLEMNFSFRYDGLGNFRVNIFRQRNDIAIVVRHVRGK
                     IENVESLHLPSVLKELILEKRGLVLVVGATGSGKSTTLAAMLEHRNNTKSGHILTIED
                     PIEYIFKHNKSIVNQREIGTDTLNYESALSSGMREAPDVLMIGEVRDRETLRHALIFA
                     QTGHLCLTTLHANNSYHALNRIVNFFPYESRQSVLSDLSMCLRAVISQRLVRNTQGKQ
                     VPAVEVLLNTSLIADMIKNDEIDQIREAIEKSVSPGSQTFEQALYKLFKSGKITKEEA
                     MRNADSSSNLSSLIDFSDRTSTMQVPVFDPAQAAPTSAKAGSDFTGIKLNLDEPK"
     gene            4523..4882
                     /locus_tag="VFW59_06925"
     CDS             4523..4882
                     /locus_tag="VFW59_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEX5363984.1"
                     /translation="MKSEVRLYGIPNCGTVKKARVWLDTHDIAYEFHDFKKHGVSETL
                     LSGWLKQIGWQNLLKKTGPTWGQLPDDVKASIVDDASALALMLEKPNVIKRPVLEQDG
                     RVLATGFNTADYEKIKP"
     gene            4922..6052
                     /gene="dapE"
                     /locus_tag="VFW59_06930"
     CDS             4922..6052
                     /gene="dapE"
                     /locus_tag="VFW59_06930"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX5363985.1"
                     /translation="MSHTLDLAKQLISRHSLTPADDGCLNIIGARLATLGFKLEKMRC
                     GEVDNLWARRGNDGPLICFAGHTDVVPTGPVARWDSDPFTPTVRDGMLYGRGASDMKG
                     SLAAFVTAIEKFVAEHPQHRGSISLLLTSDEEGVAVDGTVRVVEALQARGEMLDYCIV
                     GEPTSVARTGDTIKNGRRGSLSGVLTVKGVQGHIAYPHLVKNPIHLASPAIAELAATE
                     WDKGNEYFPPTSWQISNIHGGTGATNVVPGTVEILFNFRHSTASTVEELKARVHAILD
                     RHGLEYDLQWEMSGKPYLTPRGDLVDAVASAIKQVTGIETELSTSGGTSDGRFIADIC
                     PQVIELGPLNATIHKINECVAVADLDALSEIYCLTLHKLLVE"
     gene            6252..7154
                     /gene="prmB"
                     /locus_tag="VFW59_06935"
     CDS             6252..7154
                     /gene="prmB"
                     /locus_tag="VFW59_06935"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5363986.1"
                     /translation="MNNDFSGATHHLETVRDWLRFAVSRFNQAKLFFGHGSADPYDEA
                     AYLILHTLHLPPDQLEPFLDARLTDSERAEVLNIIQRRVEQRVPAAYLTNEAFLGDFS
                     FYVDERVIVPRSFIAELLREQLSPWIAEPEEIGSVLDMCTGSGCLAILAAHAFPNASV
                     DAVDLSPDALAVADRNVSDYGLHDQIELIESDLFTGLAGRKYDLIISNPPYVNAESVA
                     ALPKEYLHEPKLSLGSGEDGLDATRVILAHAAEHLTDNGILIVEIGHNRDALEAAYPN
                     LPFTWLEISAGDEHVFMLHRNDLL"
     gene            7154..>7249
                     /locus_tag="VFW59_06940"
     CDS             7154..>7249
                     /locus_tag="VFW59_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX5363987.1"
                     /translation="MSSVEHYENFPVASVLMPKRLRMPVAAIYHFA"
BASE COUNT         1600 a         2339 c         2054 g         1256 t
ORIGIN      
        1 cgcaggcatc gcccgggccg aattcgcagg ctttcccgtc ggcaccggtg atacaaatgc
       61 gcccttcaag cacgtaaaaa tactcgtcgc tacgttcgtc gaactcgatg cgccatgatc
      121 ccacttcaca ggcccacacg ccgcaggaca cttcgccgct ggaattaacg taatgctccc
      181 gggtggtgcg caacggattg cccgcaacca atcgctccgc tttgggatga tcgtagccgg
      241 gcgaactgtc agcctgcgca aatgagatga gtctggacat caggattctt tcaattcgga
      301 ataaagcttg ccggacaaca gactggcgga aacgatgagt gcggcgccca gccaatcgcg
      361 caacggcatc gcctcgcctg cgaggaagta ggatgcgacg gcagccacca ccagttcaaa
      421 caggaacagg atgatggcgc gattggccgg cacgcgcgcg attccatact gaacggcgaa
      481 actgctggaa cacagcacga tgccgagcag gcctatcatc agccaggatg ggccatctat
      541 caacgcgacc cgttccagaa gcgccccctg cgccagcaga aacaacccgc tcagcagcgc
      601 ggagccgagc cagacgctga aagacttgct ctccacgctc agatgcgcag cgcggcgcga
      661 gaccacattg gacagggcga agcccatgcc cgcagacaag cccacccatt cggccagact
      721 gttcggccaa ggcaggccca gttgcggctc ccacagcatg acgaacgccc ctgccagaga
      781 cagcaggatg atcgcgtaac cgtagcggtt gagccgctcc cccagcaacc agaacgaaaa
      841 caggatggtc cagaccggtg ccagatagaa cagcagcagc acgcgcatca cctcgccgtc
      901 cagtatcgcc agcatataac cgaagttcgt ccagcccgaa ctcagcacca gcaacagcgc
      961 ccaccagccg gccgcaggaa gttcgcgcca cacgcgcggc agcatgaaag ccccgctcac
     1021 catcgccagc gaataagtca gcagcgtcgt cagcgcgccg cccacacccg cgaattccag
     1081 cacccggaag ggataccaga tcaatcccca caccagtgcg ccgctcagca gtcccgcgac
     1141 cggcattaca ttttgttttg atggcactgc cctgcccctt ataatgtgcg ccgatttgtg
     1201 cgcacgatag taaacccgtc cgagagagta gatgaatccc gatttgcaac gactccagcc
     1261 ctaccccttc cagaaactcg ccaagctgtt cggcgaggta aaaccgaacc cgggatacag
     1321 cgcgatcagc ctgtccatcg gcgaacccaa gcatgccacc ccgcaattca tcaaggaggc
     1381 gctgggcgca ggcctcgacg ggctggcgaa ttatcccaca accgcaggca gcgacgcgct
     1441 gcgcggaact atttcggaat ggctgggcaa gcgctacagc atccccgcgc tcgatgcgaa
     1501 gacgcagata ctgccggtga acggcagccg cgaggcgctg ttctccttcg ctcaggcggt
     1561 gatcgaccgc agccagcccg atcccgtggt cgtctccccc aacccgttct accagatcta
     1621 cgagggtgcg gcgctgctgg gcggcgccac gccgcatttc ctcaacaccc tgccggaaga
     1681 cgactatgcg ctgaactttg cgcaactgcc ggaagccgta tggcagcgta cccagctgat
     1741 ctacgtatgc tcgccgggca atccgaacgg acatgtgatg cccttgagcg aatggcagac
     1801 gctgttcgaa atgtcggatc gttacggttt cgtgatcgcc tcggacgagt gctattccga
     1861 gatctatttc gacacgccgc cgctgggcgc gctgcaggcc gcacaacaac tcggacgctg
     1921 cgactacagg aatctggtgg tgttctccag cctgtcgaaa cgctccaacg tgccgggtat
     1981 gcgctccggc ttcgtcgcgg gcgatgcgtc catcatcgcc aagttcacgc tgtaccgcac
     2041 ctatcacggc tgcgcgatga acccggcgat ccagtccgcc agcatcgcgg cctggaacga
     2101 cgaggcccat gtcgcggaga accgccgcct gtatgcggag aaatttgcca gagtggtgga
     2161 gatactcaag ccggtgttgc cggtgacgct gcccgacgcc agcttctacc tgtggatacg
     2221 cacaccgatt gccgataccg ccttcgcgca agcgctgtat cgcgactata atgtgaccgt
     2281 tttgccgggc agctatctgg cgcgcgaggc gcgaggcatc aaccccggcg aaaactttgt
     2341 gcgtctggcg ctggtcgcca atacggaaca gaccgtggaa gccgcacaac gcatcgcaaa
     2401 atttgtctca acgcttaatt aaactctgaa cggaaaatca tcatggatca attgcagcaa
     2461 atcatcgaaa ccgcctttga acgccgcgcc gagatcaccc cgcgcaatgc cgatgcccag
     2521 ctcaaagagg cggtcgatca tgtcatcacc cagctcgatc agggcaagct gcgcgtcgcc
     2581 gagaagatcg acggcgactg ggtgacgcat cagtggctga agaaggccgt gctgctgtcc
     2641 ttccgtctcg aagacaacgc cttcatcaag ggaggattca ccaactacta cgacaaggtg
     2701 ccttccaagt tcgccgacta caacagccgc gacttccgcg aaggcggttt ccgcgtggtg
     2761 cctcctgccg ccgcacgcaa aggcgcatac atcgccaaga acgtggtgct gatgccttcc
     2821 tacgtgaaca tcggcgccta tgtcgacgaa ggcaccatgg tggacacctg ggccaccgtc
     2881 ggctcctgcg cacagatcgg caagaacgtg cacctgtccg gcggcgtcgg catcggcggc
     2941 gtgctggaac cggtgcaggc caacccgacc atcatcggcg acaactgctt cgtcggagcg
     3001 cgctcggaaa tcgtcgaagg cgtgatcctg gaagacaacg tggtgatctc gatgggcgta
     3061 ttcatcggcc agagcaccaa gatctacgac cgcgaaaccg gcgaagtgac ctacggccgc
     3121 gtgccggcag gttcggtcgt ggtcagcggc agcctgcctt ccaaggacgg cagctacagc
     3181 ctgtactgtg cggtgatcgt caagaaggtg gatgccaaaa cactgtctaa agtaggtatc
     3241 aacgaactgt tgagaggcat ctaaaaaaca ggattgagga acaatgattg aggatttaaa
     3301 aatgcagcag ttccgcctct aactccatcc tcaatcctaa ctcccaggag aatctcatga
     3361 tcattactcc gctgctgcaa ctcgctgtcg acaagcaggc ttccgacctg ttcttctcgg
     3421 tcggcgcacc ggtcaacatc aagatcgatg gcatcgccat gccggtcaac gcgcaactgc
     3481 tcgatgcgga tgtcatcaaa cgcatcgcct acgagatgat gtcgcccaag caggcggcag
     3541 aattcgaagc gaagctggag atgaacttct cgttccgcta cgacggcctc ggcaatttcc
     3601 gcgtcaacat cttccgccag cggaacgaca tcgccatcgt ggttcgtcac gtcaggggca
     3661 agatcgaaaa tgtagagagc ctgcatctgc ccagcgtact caaggagctg atcctggaaa
     3721 agcgcgggct ggtgctggtg gtcggcgcga ccggatcggg caagtccacc acgctggccg
     3781 cgatgctgga acatcgcaac aacaccaaga gcggccatat tctgaccatc gaagatccga
     3841 tcgaatacat cttcaaacac aacaagtcca tcgtgaatca acgcgagatc ggcaccgaca
     3901 cgctcaacta cgagagcgcg ttgtccagcg gcatgcgcga agcgccggac gtgctgatga
     3961 taggcgaggt gcgcgaccgc gagacgctca gacatgcgtt gatctttgct cagaccggcc
     4021 atctgtgtct gaccacgctg cacgccaaca acagctacca tgcgctgaac cgcatcgtga
     4081 acttcttccc ctacgaatcg cgtcaaagcg tgctgtccga cttgtcgatg tgtctgcgcg
     4141 cggtgatctc gcaacgcctg gtgcgcaaca cacagggcaa acaggtaccc gccgtggaag
     4201 tgctgctgaa tacctcgctg atcgccgaca tgatcaagaa cgacgagatc gaccagatcc
     4261 gcgaagccat cgagaagagc gtatcgcccg gctcccagac cttcgaacag gcgctgtaca
     4321 agctgttcaa gagcggaaag atcaccaagg aagaggcgat gcgcaatgcc gattcgtcca
     4381 gcaacctgtc ctcgctgatc gacttctccg accgcaccag caccatgcag gtgccagttt
     4441 tcgaccctgc tcaggccgcg ccgacgtcgg ccaaggcggg ttccgacttc accggcatca
     4501 agctcaatct ggatgagccc aaatgaaatc cgaagtgcgg ttatacggca tccccaactg
     4561 cggcacggtg aagaaggcgc gcgtctggct ggatacgcat gacatcgcct acgagttcca
     4621 cgacttcaag aaacacggtg tgagcgagac cctgctatcc ggctggctca aacagatcgg
     4681 ctggcagaac ttgctcaaga agaccggccc gacctggggg cagctgccgg atgacgtgaa
     4741 agcctcgatt gtggacgatg cctcggcact ggcattgatg ctggaaaagc ccaacgtcat
     4801 caaacgcccg gtgctggaac aggatggccg agtccttgct accggtttta acacagccga
     4861 ttacgaaaaa atcaaaccct gatccgtccg cagattatgc ggattcacgc agattaaaaa
     4921 catgagccac actctagact tagccaagca actcatctca cgccattcgc tcacacccgc
     4981 tgacgacggc tgcctcaaca tcatcggcgc ccgcctggca acactcggct tcaagctgga
     5041 gaagatgcgc tgcggcgaag tggacaatct gtgggctcgt cgcggcaatg acggcccgct
     5101 gatctgcttc gccggacata ccgacgtggt accgaccggc ccggtggctc gctgggacag
     5161 cgaccccttc accccgacgg tgcgcgacgg catgctgtac ggacgcggtg cgtcggacat
     5221 gaaaggctct ctcgcggcct tcgtcacagc catcgagaaa ttcgtcgccg aacatccgca
     5281 gcatcgcggc tccatctcgc tgctgctcac ctccgacgag gaaggggtgg ccgtggacgg
     5341 caccgtgcgc gtggtcgagg ccttgcaggc acgcggcgaa atgttggact actgcatcgt
     5401 cggcgagccg acttccgtag cccggaccgg cgacaccatc aagaatggtc ggcgcggctc
     5461 gctgtccggc gtactcaccg tcaagggcgt gcagggccac atcgcctatc cgcatctggt
     5521 gaagaacccc atccatctgg ccagccccgc catcgccgaa ctggcggcga ccgaatggga
     5581 taaaggcaac gaatatttcc cgccgacctc ctggcagata tccaacatcc acggcggcac
     5641 cggggccacc aatgtggtgc ccggcacggt ggagatcctg ttcaacttcc gccattccac
     5701 cgccagcacc gtggaagaac tgaaggccag ggtacacgcc atcctcgacc ggcatggcct
     5761 cgagtatgac ctgcaatggg agatgtcggg caaaccttac ctcaccccgc gcggcgatct
     5821 ggtcgatgcg gtggccagtg ccatcaagca ggttactggc atcgagaccg aactttccac
     5881 cagcggcggc acctcggacg ggcgtttcat cgccgacatc tgcccgcagg tgatcgagct
     5941 cgggccgctc aacgccacca tccacaagat caacgaatgc gtggccgtgg ccgatctcga
     6001 cgcactgtcg gagatctact gcctgacgct gcacaagctg ctggtcgaat gaaatacctc
     6061 gtactgatcc tgctcggcgt actgctgctt tggatcgcct ccgtcttctg gtccaatcgt
     6121 cgccgctccc ctgacgatcg cggaaacggc ggcgcggcag attcagggag cggcgactca
     6181 tcccactgcg ggggtgggtc ggatggggat agcggatgcg gtgatggcgg tggtgactaa
     6241 tgggaacaca aatgaacaac gatttttctg gggcaacaca tcatctcgaa acggtgcgcg
     6301 actggctgcg tttcgcggtc agccgtttca atcaggccaa gctgttcttc gggcacggca
     6361 gcgccgatcc ctatgacgag gcagcctacc tgatcctgca caccttgcac ctgccgcccg
     6421 atcagctgga gcccttcctc gatgcgcgcc tgaccgacag cgagcgcgcc gaggtgctga
     6481 acatcatcca gcgccgggtc gaacaacgcg tgcccgccgc ctacctgacc aacgaagcct
     6541 tcctcggcga cttcagcttc tatgtcgacg aacgggtcat cgtgccgcgc tccttcatcg
     6601 ccgaactgct gcgcgaacag ctcagcccct ggatcgccga accggaagag atcggcagcg
     6661 tgctggacat gtgcacaggc agcggctgtc tcgccatcct cgcggcccat gctttcccca
     6721 atgccagcgt ggatgccgtc gacctgtcgc cggatgcgct ggccgtggcg gaccgcaacg
     6781 tcagcgacta cgggctgcat gaccagatcg agttgatcga atcggacctg ttcaccggac
     6841 tggcgggacg aaaatacgac ctgatcatca gcaacccgcc ctatgtaaat gcggagtcgg
     6901 tcgccgcgct gcccaaggaa tacctgcacg agcccaagct ctctctgggc agcggcgaag
     6961 acgggctgga tgcgacccgc gtcatcctcg cacatgccgc cgaacacctg accgacaacg
     7021 gcatcctgat cgtcgagatc ggccacaacc gcgacgcact ggaagccgcc taccccaacc
     7081 tgccgttcac ctggctggaa atcagcgccg gcgacgaaca tgtgttcatg ctgcacagga
     7141 acgatttgct ctgatgtcgt cggtcgagca ctacgagaac ttccccgtcg catccgtgct
     7201 gatgcccaag cggctgcgca tgccggtggc ggcgatctat catttcgcg
//
LOCUS       DASUIV010000189         7092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_416854, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000189 DASUIV010000000
VERSION     DASUIV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416854"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFW59_06945"
     CDS             <1..972
                     /locus_tag="VFW59_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5363988.1"
                     /translation="ASSPTGGLLGSAPAMQKVRDLIERVARSQAPVHITGESGSGKEL
                     AARLIVQSGARRDKPVIAVNCGAIPEALMESEFFGYKKGAFTGADKDRDGFFQAAHGG
                     TLFLDEVADLPLTMQVKLLRAIQEKRVRKVGATAEDAVDVRLISATHNDLAMRVQQGM
                     FRQDLFYRLNVIQIQMPPLRELREDIGAIAQLTLERLRGGSPVEFSDEALRTLRGYDF
                     PGNVRELENIVERALALCSGGVISVEDLQLSPAGQVHTESTELGDKSPLPDYLDRVER
                     QALLEALHRTGFNRTAAAKLLGLTFRTMRYRMERLGIKDPDGSHDAD"
     gene            962..1534
                     /gene="ampD"
                     /locus_tag="VFW59_06950"
     CDS             962..1534
                     /gene="ampD"
                     /locus_tag="VFW59_06950"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEX5363989.1"
                     /translation="MRINAQGLLEGGEYIPSPNRDARLAGEIDLLVIHNISLPPGEFG
                     GDGVRRLFTNTLDVNAHPYYQTIAGLRVSAHFFVRRDGHVTQFVSCLERAWHAGVSSW
                     QGRERCNDFSIGIELEGCDDRPFSEAQYRALGELSCALRAAYPIRGIAGHSDIAPRRK
                     TDPGPGFDWKRYLIGLSESQLRIQGIDKNI"
     gene            1699..4512
                     /locus_tag="VFW59_06955"
     CDS             1699..4512
                     /locus_tag="VFW59_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX5363990.1"
                     /translation="MHVTGVSDSIQSQPSSFDQYKVIRRNGSVVPFEPGKISVALTKA
                     FIAVNGGQGAGSARVRELVEQLTGSVVSALMRRQPHGGTLHIEDIQDQVELGLMRSGE
                     HDVARAYVLYREERSRSRAKVKSAEPVHVINVKGVDGVLRPLDMTRLSDLVISACEGL
                     GDVVGADAVLKLAVKDLYDGVTQHEVSKCLIFAARSLIEQEPAYNFVTARLLLNNICC
                     ETLGEEVAPKEMATRYAEYFPGYIKRGVAADLLDARLAQFDLPRLAAELDATRDLQFG
                     YLGLQTLYDRYFLHIEGERIELPQAFFMRVAMGLALNEIDREARTIEFYRLLSSFDFM
                     SSTPTLFNSGTQRSQLSSCYLTTVADDLDGIYEAIKENALLAKYAGGLGNDWTPVRAL
                     GAHIKGTNGESQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLETWHLDIEEFLDLRKN
                     TGDDRRRTHDMNTSNWIPDLFMKRVMEGGDWTLFSPSNVPDLHEKFGAAFEQAYVAYE
                     AKAASGEIKLYKKVPAASLWRKMLTMLFETGHPWVTFKDPCNIRSPQQHVGVVHSSNL
                     CTEITLNTSESEIAVCNLGSVNLTAHLYPAGHEKAGQLDHEKLRRTVSTAMRMLDNVI
                     DINYYAVAKARNSNLKHRPVGLGIMGFQDCLHELRIPYSSQEAVEFADRSMEAVCYFA
                     YMASTELAEERGRYASYRGSLWDRGILPQDSLELLRQERGGYVEVDSSTSMDWDILRE
                     RIKQFGMRNSNCVAIAPTATISNIIGVSACIEPTYQNIFVKSNLSGEFTVINNHLVSD
                     LKQLGLWDEVMIADLKYFDGTLAKIDRIPQELRSLYATAFEVEPSWLIEAASRRQKWI
                     DQAQSLNIYMAGVSGRKLDETYKLAWLRGLKTTYYLRTLGATSAEKSTGRAGALNAVP
                     THGGVVEETQFCSIDNPECEACQ"
     gene            4854..5999
                     /locus_tag="VFW59_06960"
     CDS             4854..5999
                     /locus_tag="VFW59_06960"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX5363991.1"
                     /translation="MLTFEDDIKTTTAQFGGMGAAYGKLPVNPQHDDLRMDSADQALT
                     ARVRVEDKAIINSRADVNQLVPFKYKWAWEKYLAACANHWMPQEVNMTADIALWKSNK
                     LSDDERRLVKRNLGFFTTADSLAANNIVLGTYRHISNPECRQFLLRQAFEEAIHTHAY
                     QYIVESLGLDDGEIFNMYHEVPSIRAKDEFLIPYINVLTDPAFKTGTPEADQALLRSL
                     IVFACIMEGLFFYVGFVQILALGRQNKMTGAAEQYQYILRDESMHLNFGVDVINQIKM
                     ENPHLWTSEFREEIRSLMQKGVELEYAYAEDTMPRGVLGLNSNMFKEYLRFIANRRCQ
                     QIGVDVLYPGASNPFPWMAEMIDLKKEKNFFETRVTEYQSGGALSWD"
     gene            6820..>7092
                     /locus_tag="VFW59_06965"
     CDS             6820..>7092
                     /locus_tag="VFW59_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5363992.1"
                     /translation="MAAEKKTKPVAKKAAAVKKPVAKKVVAKPAAKKVVAKKVAAKPA
                     AKKVVAKKVAAKPAAKKVVAKKVAAKPAAKKVVAKPAVKVAAKPAAK"
BASE COUNT         1583 a         1688 c         2163 g         1658 t
ORIGIN      
        1 gcctcttcgc cgacgggagg gctgcttgga tccgcgccag cgatgcagaa ggtgcgagat
       61 ttgatcgagc gcgtcgcacg aagtcaggcg ccggtacata ttactggcga gtcaggcagc
      121 ggcaaagagc tggcagcccg cttgattgtg caaagcggcg cgcgacgcga caagcccgtt
      181 atcgcggtga actgtggtgc gattcccgaa gcgctgatgg agagcgagtt tttcggctac
      241 aagaagggcg cattcaccgg ggccgacaag gacagggatg gcttctttca agcggctcat
      301 ggcggcacgc tgtttctgga cgaggtggcg gacttgccgc tgaccatgca ggtcaagttg
      361 ttgcgcgcaa ttcaggagaa acgcgtgcgc aaagttggcg ccactgctga ggatgcggtg
      421 gacgtgcgcc tcattagtgc aacgcataat gatttggcca tgcgcgtgca gcaagggatg
      481 ttccgtcagg atctgttcta tcggttgaat gtcatccaga ttcagatgcc tcccttgcga
      541 gagttgcgcg aagatatcgg agccatcgcg cagctaacgc tggaaagact gcgtggtgga
      601 tcgcctgtag aattttcgga tgaggcgctg cggactttgc gcggctacga ttttccggga
      661 aatgtgcgcg agctggaaaa tatcgtcgaa cgcgctctgg cgctatgttc cggaggggtg
      721 atctctgtgg aggacctgca attgtcaccg gcaggtcagg tgcatacaga gtccaccgag
      781 ttgggggaca agagtccgct acccgactat ctggatcggg tggagcgtca ggcgttgctg
      841 gaggctctgc acagaaccgg cttcaatcga actgcggcgg ccaagctgct gggattgacg
      901 ttccgcacga tgcgctatcg catggagcga ttgggcatta aggacccgga cggcagccat
      961 gatgcggatt aacgcgcaag ggctgctgga aggcggggaa tatatcccct ccccaaaccg
     1021 tgatgcgcgt ctcgctggtg aaatcgattt gctggtgata cataacatca gcttgccgcc
     1081 gggagagttt ggcggtgacg gtgtgcggcg cctgttcacc aatacactgg atgtcaatgc
     1141 tcacccctat taccagacca ttgcggggtt aagggtgtcg gcgcattttt tcgtgcgtcg
     1201 ggatggtcat gtgactcagt tcgtctcctg ccttgagcgc gcctggcatg ccggagtgtc
     1261 atcttggcag gggcgcgaac gttgtaatga tttttccatc gggatcgaat tggaggggtg
     1321 cgatgaccgg ccgttttccg aggcacagta tcgcgccttg ggtgagttga gctgcgcctt
     1381 gcgagcggcg tatccgatac gaggcatcgc gggccatagc gatatcgcgc cccggcgcaa
     1441 gacagatccg ggacctggtt tcgactggaa gcgctatctg attggactct ctgaaagtca
     1501 actccgtatt cagggaatag ataaaaatat ataggcattt tttctgcttg caatattccg
     1561 cttggacact acaattagct ccgccgctca aaaaaaatac tagatatagt ggtttttggc
     1621 tttcagggtt aaatcagatt tcaagggagt aagcttatgc tgcatgctgt agccgccgat
     1681 ggtgtttcca tgtcttcaat gcatgtcacg ggcgtttccg attccattca gtcccaaccg
     1741 agttcgttcg atcagtacaa ggtgatccgt cgcaacggtt cggttgttcc gttcgaacct
     1801 ggcaaaattt cagtcgcttt gaccaaggcg ttcatcgcgg tgaacggtgg gcagggggcg
     1861 ggctctgccc gtgtgcgcga gctggtcgag cagctgaccg gaagtgtggt ttcggcactg
     1921 atgcgccgtc agccgcatgg cgggacgttg cacatcgagg atattcagga tcaggtcgaa
     1981 ctgggtctga tgcgttccgg tgagcatgac gtggcgcgcg cctatgtgct gtaccgtgaa
     2041 gagcggtctc gttcccgggc caaggtcaag agcgcggagc ccgtgcatgt catcaatgtg
     2101 aagggcgtgg atggggtctt gcgcccgttg gatatgacgc ggctgtccga tctggtgata
     2161 tccgcctgcg aaggcttggg tgatgtggtc ggggcggatg cggtgcttaa gctggcggtc
     2221 aaagacctat atgatggcgt aacgcagcat gaagtcagca agtgcctgat cttcgccgcg
     2281 cgctcgttga tcgagcagga gccggcctat aactttgtga cagcccgcct gttgcttaac
     2341 aatatctgct gcgagacatt gggtgaggag gtcgcgccaa aagaaatggc gacgcgttat
     2401 gcggaatatt tccccggtta tatcaagcgt ggtgtggcgg cagatttgct ggatgcgcgt
     2461 ctggcgcagt tcgacttgcc gcgtctggcg gcagaattgg atgcaacgcg cgatctgcag
     2521 ttcggctatc ttggtttgca gacgctgtat gaccgttact tcctgcatat cgagggtgag
     2581 cgcatcgagt tgcctcaggc cttctttatg cgggtggcga tgggtttggc gttgaacgag
     2641 atcgatcgcg aggcgcgtac catcgagttt taccgccttc tttccagctt cgatttcatg
     2701 agctctacgc cgaccctgtt caattcgggt actcagcgtt cccagctttc ttcctgttat
     2761 ctgaccaccg tggcggatga cctggacggc atctacgagg cgatcaagga gaacgcgctg
     2821 ctggccaagt atgctggcgg gctgggtaac gactggacgc cggtgcgcgc gctcggtgcg
     2881 catatcaagg gaaccaatgg cgagtcgcag ggcgtggtgc cgttcctcaa ggtggtgaac
     2941 gatacggcgg tggcggtgaa ccagggcggc aaacgcaagg gggcggtgtg cgcttacctc
     3001 gagacctggc atctggatat cgaggagttt ctggatctgc gcaagaacac gggcgatgat
     3061 cgtcgccgca cgcatgacat gaatacttcc aactggattc ccgacctgtt tatgaagcgg
     3121 gtaatggaag gcggcgactg gaccttgttc tccccgtcca acgtacctga tctgcacgaa
     3181 aagtttggcg cagccttcga gcaggcttat gtggcctacg aggcaaaagc ggccagcggc
     3241 gagatcaagt tgtacaaaaa ggtgcctgcc gccagtctgt ggcgcaagat gctgaccatg
     3301 ttgttcgaga ccggccatcc ttgggtcacg ttcaaggatc cttgcaacat tcgttcgccg
     3361 cagcagcacg tcggcgtcgt gcatagttct aatctgtgta ccgagattac gctgaacacc
     3421 agtgagagcg agattgccgt gtgcaacctg ggctcggtca acctgaccgc tcacctgtat
     3481 cctgccgggc atgagaaggc tggtcagctc gatcatgaaa agctgcgtcg cactgtcagt
     3541 accgcgatgc gtatgctgga taacgttatc gatatcaact attacgcagt cgccaaggcg
     3601 cgcaattcca atctgaagca ccgcccggtc ggtctgggca tcatgggctt tcaggattgc
     3661 ctgcacgagt tgcgcattcc ttattcctct caggaggctg tcgagttcgc cgatcgctcc
     3721 atggaggcgg tgtgctactt cgcttacatg gcatcgaccg agttggcgga agagcgcggt
     3781 cgctatgcga gctatcgcgg cagtctgtgg gatcgcggca tcctgccgca ggattctttg
     3841 gagttgttgc gccaggagcg tggtggttat gtcgaggtgg atagttccac cagcatggat
     3901 tgggacatct tgcgtgaacg catcaagcag ttcggcatgc gtaactccaa ttgcgtggca
     3961 atcgcgccga cggcgacgat ctccaatatt atcggggtgt ctgcctgtat cgagccgacg
     4021 taccagaaca tattcgtgaa atcgaatctg tctggagagt tcaccgtgat caacaatcac
     4081 ttggtgtcgg atttgaagca gcttggattg tgggatgagg taatgatcgc cgatctgaaa
     4141 tatttcgatg ggacactggc gaagatcgat cgtatcccgc aggaattgcg cagtctctac
     4201 gcaacggcat tcgaagtcga accgagctgg ctgattgagg cggcatcgcg ccgacagaag
     4261 tggatcgatc aggcgcaatc gctgaatatc tacatggcag gcgtgtcagg tcgaaaactg
     4321 gatgaaacct ataagctggc gtggctgcgc ggcctgaaaa ccacctatta tctgcgcacc
     4381 ttgggggcga cctctgcaga gaaatccact ggacgagccg gtgcattgaa tgcggttcca
     4441 actcatggtg gcgtggtgga agagactcag ttctgctcga ttgataatcc agagtgtgag
     4501 gcgtgccagt aggctcatcg cacgcactgg attgcggcga agactaacct tcgggttatg
     4561 tcggagccac aatccagcga agcatgtcag tgacatgctg gcttcaggat tacggcgaag
     4621 gctctcgtgc ccggagggtg aacttgtgta gcaagttaat ctgccaaaca gcggccggag
     4681 ctgtgcgaag cctgccaata ggccctgggt tgcggcgaag gctaaccttc atgtcggagc
     4741 cacaatccag cgaagcctgt cggtagatcg atgcgcaatt tgcgtagcgg caagccggag
     4801 cagcaaatca gaacaaatac gggcagcaat gcccaatgaa aacaggagaa aacatgctga
     4861 cattcgagga cgatatcaag acgaccacag ctcagttcgg cggtatgggc gctgcctatg
     4921 gcaagttgcc cgtcaatccg caacatgacg atctgcgcat ggattccgcg gaccaggctc
     4981 tcactgctcg agtgcgtgtc gaagacaagg cgatcatcaa ttctcgtgcc gacgtgaatc
     5041 agttggttcc gttcaagtac aagtgggcgt gggagaagta tctcgctgct tgtgccaatc
     5101 actggatgcc acaggaagtg aacatgactg ccgacatcgc gctgtggaag agcaataaac
     5161 tgagcgatga cgagcgtcgt ctggtcaagc gtaatctcgg tttcttcact acggcagata
     5221 gtctggctgc gaacaacatc gtgctgggaa cctaccgcca catcagtaat cctgagtgcc
     5281 gccagttcct gttgcgtcag gcgttcgaag aagcaatcca tactcatgcc tatcagtaca
     5341 tcgtcgaaag tctgggcttg gatgatggcg aaatattcaa catgtatcac gaggttccga
     5401 gtattcgtgc caaggatgag tttctgatcc cgtatatcaa tgtattgact gatcctgcat
     5461 tcaagaccgg caccccggaa gcggatcagg cattgttgcg cagcctgatc gtgtttgcct
     5521 gcatcatgga aggcttgttt ttctatgttg gttttgtgca gattctggcg ttggggcgac
     5581 agaacaaaat gaccggtgct gccgagcagt accagtacat tctgcgtgac gagtccatgc
     5641 atttgaattt tggtgtggac gtgatcaacc agatcaagat ggagaacccg catctgtgga
     5701 ccagtgagtt ccgcgaggag attcgcagcc tgatgcagaa gggtgtggag ttggaatatg
     5761 cctacgcaga agacaccatg ccacgcggcg tacttggact gaattccaat atgtttaagg
     5821 aatatttgcg ttttatcgct aaccggcgtt gccagcagat tggtgtggac gtcttgtatc
     5881 cgggggcgag caatccgttc ccctggatgg ctgagatgat cgatctcaag aaagaaaaga
     5941 atttctttga gactcgcgta accgaatacc agagcggcgg tgcgttgtct tgggattaag
     6001 gcaggtaaat tgttcgcgga cagataatcc tgccttgcag cgttgttaag ttcgttaaaa
     6061 attaaaaggg ctccgctttg aaagctaggg ccctttttta ttggtgctcg gtttgagcag
     6121 gaattatgtc gttaatattt ccttgaggaa gatggtgtca aatcctggct ttttattgag
     6181 ccagtgcata accatatgcg gtcaggattt ccgtgctaag gatggtggct gacagcttgg
     6241 gagcaagcaa ttgaaagagg ctatgcgtgc gtaggtttgg aagagttgat aagacgagtg
     6301 attcgaattg gaacaataaa tcgtatctgt taaacacatc aaatgtgttg acatggatca
     6361 caaatccgtt aaagtcgcaa ccgcaatatg tagtaataaa ttctttccga tttactagtt
     6421 gtggttctat gaaaaaaggg ttttcgcttg tgcgaaatag acagtcataa gcgtggatgt
     6481 ttttctccgc gtgttgcgcc agaagcgcaa atgaatagcg agattgagtc tcgaggattt
     6541 tctgtctctc agcttgggcg acgttgggtt ggttttaaat aacgcgtcac cgttaacaga
     6601 acggtggcgc gttgttttgt gcgccgcata attttggttg ttgtgatggg tttatcagcc
     6661 aggttgctgg ctaaatttat agccaatcga ctaagccggc aaagaacgct gactaggtcg
     6721 ctggttaaag agcttcggtc atgccgcagt gcgacatgtt gagggtggta gttgcaggtg
     6781 ttgtatttcg ttaactttca ttatcgcaag gaggttgaaa tggcagcaga gaagaaaact
     6841 aaaccagtgg caaaaaaggc tgcggcagtt aaaaagccgg tagctaaaaa ggttgtagcc
     6901 aagccagcgg ctaagaaggt tgtagccaag aaagtcgcag ccaagccggc ggccaagaag
     6961 gttgtagcca agaaagtcgc agccaagcca gcggccaaga aggttgtagc caagaaagtc
     7021 gcagccaagc cagcggctaa gaaggttgta gcgaagccag cagtcaaggt tgcagcgaag
     7081 ccagcagcca ag
//
LOCUS       DASUIV010000190         6823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1254609, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000190 DASUIV010000000
VERSION     DASUIV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254609"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFW59_06970"
     CDS             <1..548
                     /locus_tag="VFW59_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX5363993.1"
                     /translation="RLRGQSLSYYGMQLAHLGVAVFIIGVTLVKGYETERDVRMNVGD
                     TLQAGGYEFRFNGVTEEQGPNYVAGRGSVTVTQDSKLITELFPEKRQYNASGMPMTEA
                     SIRPGLLRDLYVSLGEQIPDSEGAWAVRVYIKPFVDWIWAGCLFMALGGVLAISDRRY
                     RIHSKKTNQSTAELPLEEKTA"
     gene            545..1099
                     /locus_tag="VFW59_06975"
     CDS             545..1099
                     /locus_tag="VFW59_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX5363994.1"
                     /translation="MMRFILPFVIFVALAAFLYKGLSNVNHNLEQGKDPREIASPLIN
                     KAAPDFSLPLLHEPAKQFSPQEMKGKVWLLNVWASWCGACKDEHPLLMELSRQNILPI
                     VGVDYKDKREDGEATLSKVGDPYTLVIADADGKAFFNYGVYGVPETYLIDKQGIIRYK
                     LTGAISAQNWKNQMLPLIKELQAK"
     gene            1096..1572
                     /locus_tag="VFW59_06980"
     CDS             1096..1572
                     /locus_tag="VFW59_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX5363995.1"
                     /translation="MKYLSILMMCLLPAFSFAGEAKDIADDPVMEARMIKLAEKVRCL
                     VCQSEPVSTSHSDWSNDVRAIMREKMKAGATDQEIMDMLVERFGKSVLFDPPVDKETM
                     PLWIAPFALLLIGGGVLVYQLRKRKGLVAAEPQLSDEDSRRAADLLDQPNKKDTQA"
     gene            1569..2831
                     /gene="ccmI"
                     /locus_tag="VFW59_06985"
     CDS             1569..2831
                     /gene="ccmI"
                     /locus_tag="VFW59_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX5363996.1"
                     /translation="MTLFWIVCAVLLLIALLFLVLPLWRATANNNDVLRDAANLEILR
                     DQSTEMEADLRNGLLTQEAFEQGKRELQARLLDEVKVTEQATRAPRNPAKVLAIVLAV
                     VLPLCSVSLYLTIGNLKALQPKSELAMAEGFGVLRSEEALQALEKKMERLPENPDGWL
                     QLARSYSELQRYPDAVRAYQNLVKLVPNESQLWTDYADAAAMNNGQSLKGDPTKFLDK
                     ALQLDGNNTSALALSGSAAMERGDYFAAVVHWQKLVELLPADYPDVQMIHDGINQAKE
                     FLAMQKGGKQKLAQLATMKGAAETKPAADPALAITGKVSLSPALAAKVAPTDIVFILA
                     RAANGPKMPLAVLRKQVKDLPMEFTLDDSMAMQPQLKLSGFDQVVVVARVSKSGSPMG
                     QPGDLEGLVQSAKPGSKGLNIVIDTVVK"
     gene            3077..4201
                     /locus_tag="VFW59_06990"
     CDS             3077..4201
                     /locus_tag="VFW59_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEX5363997.1"
                     /translation="MLAADICAGATPRELRCKGEWLLRSVAQIDARIHACLESASRPV
                     GGEWVIDAQAISALDTSGAWLLHRTRRDLELGGGTVRFVGLRPEFEALLGLIALRTQS
                     SAIEPAIASGILTRIGRRVWVSLLGAFGLLSFLGASATALMHAVMQPQHIRWKSILYN
                     LQTTGVAALPITGLLSFLLGIVIAYQGAEQLSRVGANIYIVDLVGLSMVRELSPLITA
                     IIIAGRSGSAYTAQIATMKVTEEVDALRTIGVGPLDLLVLPKVIALVIALPLLTVYTD
                     IMGVLGGMVMARAQLDVGFITFFDRLGDAVSLTSFLIGIGKAPVFAVIIALVGCYQGF
                     QVSGSAESVGQQTTKSVVQSIFLVILADALFSIAFSWLGI"
     gene            4198..4986
                     /locus_tag="VFW59_06995"
     CDS             4198..4986
                     /locus_tag="VFW59_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5363998.1"
                     /translation="MRTAVDTAVIEIRDLNTRFGDAVVHRDVSLSVRRGEIFALVGGS
                     GCGKSTMLREIIMLQPPESGSIRVFGQEVIGLSDEQALPFRRRWGVMFERGALFSALT
                     VMENVAMVIREHTGLEPALVDELAALKIALTGLPADAGQKYPSELSGGMRKRAALARA
                     LALDPELLFLDEPTAGLDPLSASGIDELVRYLRDALGLTIMMVTHDLDLLWRVADRVA
                     VLGDGRILGEGTMQELARSEDALIREYFYGPRGRGALEQAWNRK"
     gene            4971..5807
                     /locus_tag="VFW59_07000"
     CDS             4971..5807
                     /locus_tag="VFW59_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5363999.1"
                     /translation="MESKVNFAVVGLFVLILGALLIGGVLWFSGGNSYGKTYDTYLVY
                     MSESVSGLSLDAPVRYRGVQVGGVRRIELSPGNAERVQLTLDIERGTPVKQDTVAVLQ
                     VQGLTGIAHVDLSGGSRNSPPLKAGPDEKYPVIATGPSLMLRLDTAVTALLTNLNRSS
                     ENINALLDQENRVALRHTLASLDKLSGAMAGADLPQLLQRLQRSADAFDRMANETARA
                     GASTAGAVESVRAEALPEARQAIAELRELTASLRRFSEALEHNPGMLLQGRENAKRGP
                     GE"
     gene            5831..6466
                     /locus_tag="VFW59_07005"
     CDS             5831..6466
                     /locus_tag="VFW59_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX5364000.1"
                     /translation="MKSNDTTWLARFAALAPIAVILALLGGCSVLPSAPEPDNLYLLE
                     AGKAPARHVAAARRDLVLAVSMPRARAGFATAQMIWVRQAHELEAYSHNRWADTPARM
                     LAPLLVQTLERSGTFHAVVQSPSSVAATWRLDTELIRLQQDFTVKPSEVRFTLGAQLI
                     DIATQRVIASAQFDESEKSDTEDAYGGVLAANRALERLLVRLAEFCAENAR"
     gene            complement(6620..>6823)
                     /locus_tag="VFW59_07010"
     CDS             complement(6620..>6823)
                     /locus_tag="VFW59_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364001.1"
                     /translation="EDTRHSGGSLFWESQYRAMHGGDFFAERIAVHTPRLYGSMQFPS
                     EIALIEAIGDESDDAHGQLTAMR"
BASE COUNT         1274 a         1946 c         2219 g         1384 t
ORIGIN      
        1 ctcgtctgcg cggccaatcc ttgagctatt acggcatgca gttagcccac ctcggcgtgg
       61 cggtgttcat catcggtgtg accctggtca agggctacga aaccgagcgc gatgtgcgca
      121 tgaatgtggg cgatacgctg caggcgggcg gttacgagtt ccgcttcaac ggcgtcaccg
      181 aagagcaggg gcctaactat gtggcaggac gcggcagtgt gacggtcact caggatagca
      241 agctgatcac cgagctgttc ccggagaaac gtcagtacaa cgcgtccggt atgccgatga
      301 ccgaggcttc gatccgcccc ggcctgctgc gcgacttgta cgtgtcgctg ggcgaacaga
      361 ttcctgacag cgagggtgcc tgggcggtgc gcgtctacat caagccgttc gtggactgga
      421 tctgggccgg ttgtctgttt atggcgctgg gcggcgtgct ggcgatcagc gatcgtcgct
      481 atcgcattca cagcaagaag accaatcagt cgacggccga attgccgctg gaggagaaga
      541 ccgcatgatg cgtttcatat tgccgtttgt gatttttgtg gcgttggctg ccttcctgta
      601 caaggggctg tcgaacgtta accataatct cgagcaggga aaagacccgc gcgagattgc
      661 ctcgccgctg atcaacaagg ctgcgccgga tttctcgctg cctttgttgc acgagcctgc
      721 caaacaattc tcgccgcagg aaatgaaggg taaggtctgg ttgctgaacg tctgggcatc
      781 ctggtgcggt gcctgcaaag acgagcatcc tttgctgatg gaattgtcgc gccagaacat
      841 cctgccgatc gtcggtgtgg actacaagga caagcgcgaa gacggcgagg cgaccctgag
      901 caaggtgggc gatccctata cgctggtcat cgccgatgca gacggcaagg cattcttcaa
      961 ctatggtgtg tatggtgttc cggagactta tcttatcgat aagcagggca tcatccgcta
     1021 caagttgacc ggcgccatca gcgcgcaaaa ctggaaaaat cagatgttgc cgctgatcaa
     1081 ggagttgcag gcaaaatgaa atacctttcc attctgatga tgtgtctgct acccgcattc
     1141 tcgttcgcgg gcgaagcgaa agacattgcc gacgatccgg tgatggaggc tcgcatgatc
     1201 aagctggccg agaaggtgcg ctgtctggtc tgtcagagcg agccggtgtc cacctcgcat
     1261 tccgactggt cgaacgacgt gcgtgcaatc atgcgagaga agatgaaggc cggggcgacc
     1321 gatcaggaaa tcatggacat gctggtcgag cgtttcggca agtcggtact gtttgatccg
     1381 cccgtggaca aggaaaccat gccgctctgg attgcgccgt tcgcgctgtt gctgatcggc
     1441 ggcggtgtgc tggtctatca gttacgcaag cgcaagggct tggtcgctgc cgagccgcaa
     1501 ctctccgatg aggactcgcg ccgtgcggcc gatttattgg atcaaccgaa taagaaagac
     1561 acacaagcat gactctgttc tggatcgtct gcgcagtact gctgctgatc gcactgctgt
     1621 ttctggtgct gcccctgtgg cgcgcaaccg ccaataacaa cgatgtgctg cgcgatgcgg
     1681 ccaatctgga gatattgcgc gaccagtcga ccgaaatgga ggcggacctg cgcaatggcc
     1741 tgctgacgca ggaggccttc gagcagggca agcgcgaatt gcaggcgcgc ctgctggacg
     1801 aggtcaaggt caccgagcaa gcaactcggg cgccacgcaa tcccgcgaag gttctggcta
     1861 tcgtgctggc cgtcgtgctg ccgctgtgca gcgtgtctct ctatctgacc atcggcaacc
     1921 tcaaggcctt gcagccgaaa tccgaactcg ctatggccga gggtttcggt gtgctccgtt
     1981 ccgaggaagc attgcaggcg ctggagaaaa agatggagcg tctgccggag aatccggacg
     2041 gctggttgca gctggcgcgt tcctacagcg agttgcagcg ttatcccgat gcggtgcgcg
     2101 cttaccagaa tctggtgaag ctggtgccga acgagtcgca gttgtggacc gactatgccg
     2161 atgctgcggc gatgaacaac ggccagtcgc tgaagggtga tccgaccaag ttcctcgata
     2221 aggcattgca actcgacggc aacaacactt ccgcgctggc actgtccggt tcggcggcga
     2281 tggagcgcgg cgattacttt gcggccgttg ttcattggca aaagctggtc gagttgttgc
     2341 cggcggacta tccggatgtg cagatgattc acgacggcat caatcaggcc aaggagtttc
     2401 tggccatgca gaagggcggc aaacagaagc tggctcagct cgcgaccatg aagggagctg
     2461 ccgagacgaa gcctgcggcc gatcctgcac tggcgatcac cggcaaggtc tcgttgagcc
     2521 cggcgctggc cgccaaggtt gcgccgaccg acatcgtgtt catcctcgcg cgcgccgcga
     2581 atggcccgaa gatgccgctt gcggtgttgc gcaagcaggt gaaggacctg ccgatggagt
     2641 tcacgctgga cgacagcatg gccatgcaac cgcagctgaa gctgtccggt ttcgatcagg
     2701 tggtggtggt cgcgcgcgtg tcgaagtccg gcagcccgat gggccagccc ggcgatctgg
     2761 aaggtctggt gcaatccgcc aagccgggca gcaaaggcct gaatatcgtg atcgatactg
     2821 tcgtgaagta acggaacgac agctgttcca ttaaggcatc cggtcgcgag accggatgcc
     2881 ttttttcatt cgggatgccg tccttgcgca tccatttgac aggccatagc gtgcggcata
     2941 gcatccctct ggaagcacgg cgatcgctag aaccgagaag cactgaccgt ttgccctgtg
     3001 ctgtcatacc gtatttcaat ccgatagtgc catcaggatg attaacgcgc cgatcacctc
     3061 acgttccacc gcagcaatgt tggcggcaga tatctgtgcc ggtgcgacac cgcgcgagtt
     3121 gcgctgcaag ggggagtggt tgttgcgttc tgtggcgcag atcgatgccc gcatccatgc
     3181 gtgtctcgaa tcggcgtcac gacctgtcgg cggcgagtgg gtgatcgatg cgcaagccat
     3241 cagcgcgctg gatacctcgg gtgcctggtt gctgcatcgc acccggcgcg atctggagct
     3301 cggcgggggg actgtacgct ttgtcggact gaggccagaa ttcgaggcgc tgctcggtct
     3361 gatcgccctg cgtacgcagt cttcggccat tgaacccgcg atagcgtcgg gaatcctgac
     3421 ccgcatcggg cgcagggtat gggtgagttt gctcggcgcg ttcgggctgc tgtcatttct
     3481 cggagccagc gcgaccgcac tgatgcatgc cgtgatgcag ccgcagcata tccgctggaa
     3541 atccatcctg tataacctgc agaccaccgg cgttgcggcg ctgccgatca ccggcttgct
     3601 gtcttttctg ctcggtatcg tgatcgccta tcagggtgcg gagcagctga gtcgcgtcgg
     3661 cgccaacatc tatatcgtcg atctggtcgg cctgtcgatg gtgcgcgagc tctcgccgct
     3721 gatcaccgcg atcatcatcg cgggccgttc cggttctgcc tatacggcgc agatcgccac
     3781 catgaaggtg accgaggagg tcgacgcatt gcgtaccatc ggagtcggcc ccctcgacct
     3841 gctggtgctc cccaaggtga tcgcgctggt gatcgcgctg ccgttgctga ccgtctatac
     3901 cgacatcatg ggggtgctcg gcgggatggt catggcacgc gcccagctcg atgtcggttt
     3961 catcaccttc ttcgaccgtc tcggcgatgc ggtcagtctg acttcgttcc tgatcggtat
     4021 cgggaaggcg ccggtgttcg cggtgatcat cgcgctggtc ggttgctatc aggggtttca
     4081 ggtgagcggc agcgccgaga gcgtcgggca gcagaccacg aaaagcgtgg tgcaatcgat
     4141 ctttctggtg atcctggccg atgcgctgtt ttccatcgct ttcagctggc tgggcatatg
     4201 agaaccgccg tcgacaccgc agtcatcgag atacgggacc tgaacacgcg tttcggcgat
     4261 gcggtcgtac accgtgacgt gagtctttcg gtgcggcgcg gcgagatatt cgcgctggtc
     4321 ggcggcagcg gctgcgggaa gtcgaccatg ctgcgcgaga tcatcatgct gcagccgccg
     4381 gagtcgggct cgatccgggt gttcggtcag gaggtgatcg gactttccga cgagcaggcc
     4441 ttgccgttcc ggcgccgctg gggggtgatg ttcgagcgcg gcgcgttgtt cagcgcgctc
     4501 accgtgatgg agaacgtcgc catggtgatc agggagcaca ccggccttga accggcactg
     4561 gtcgacgagc ttgccgccct caagatcgcg ctcaccgggc tgcctgccga cgcggggcag
     4621 aagtacccga gcgagctttc cgggggaatg aggaagcgtg cggcgctggc gcgcgcgctg
     4681 gcactcgatc cggagctgct gtttctggac gaaccgacgg ccggtctcga tccgctgagt
     4741 gccagcggca tcgacgaact ggtgcgctat ctgcgcgacg cgctcggcct caccatcatg
     4801 atggtcaccc acgacctcga tctgctgtgg cgcgtggcgg atcgtgttgc agtgctggga
     4861 gatgggcgta ttctggggga agggacgatg caggagttgg cgcgctctga agatgcgctg
     4921 atacgggagt atttttacgg tcctcggggc agaggagcat tggagcaagc atggaatcga
     4981 aagtgaattt cgcggtggtc gggctgttcg tgttgatact cggcgcgttg ctgatcggcg
     5041 gcgtgctgtg gttctcgggc ggcaactctt acggcaagac ctacgatacc tatctggtct
     5101 atatgagcga atcggtatcg ggcctgagtc tggatgcgcc ggtgcgttat cgcggtgtgc
     5161 aagtcggcgg cgtgcgccgc atcgagctgt cgccgggtaa cgccgagcgg gtgcagctca
     5221 cgctggatat cgaacggggc acgccggtga agcaggatac ggtggccgtg ctgcaagtgc
     5281 aggggctgac cggcatcgcg catgtcgatc tttccggcgg cagccgcaat tcaccgccgc
     5341 tgaaggccgg cccggatgag aaatacccgg tgatcgcgac cgggccgtcg ctgatgttgc
     5401 gtctggatac cgccgtgacc gcgctgctca ccaatctcaa ccgcagcagc gaaaacatca
     5461 acgcgctgct ggatcaggaa aatcgtgtcg ccctgcgcca cacgctggcc agtctggaca
     5521 agctgtccgg cgcaatggcc ggtgccgatc tgccgcaact gctgcagcgc ctgcagcgca
     5581 gtgcggatgc cttcgaccgg atggcgaatg aaaccgcccg tgccggagcg agcactgcgg
     5641 gcgccgtcga gagcgtgcgc gcggaagcct tgccggaagc gcgtcaggcg attgccgagc
     5701 tgcgcgaact gacggcctcg ctgcgccgct tcagcgaggc gctggagcat aatcccggca
     5761 tgctgttgca gggccgggaa aatgcgaaac gcggtccggg cgagtgacac cctataacgg
     5821 agtcgacaat atgaaatcca acgatacgac atggctcgcc cggttcgccg cactggctcc
     5881 gattgcagta atcctggcgc tgctgggcgg ctgttcggtt ttgccctctg cgccggaacc
     5941 ggacaacctc tatctgctgg aagccggaaa ggccccggct cgtcacgttg cggcggccag
     6001 gcgcgacctc gtgcttgcag tcagcatgcc acgtgcccgt gcaggcttcg ctaccgccca
     6061 gatgatctgg gtgcgtcagg cgcacgaact cgaggcctat tcgcacaacc gctgggcgga
     6121 tacaccggca cgcatgctgg caccgttgct cgttcagacg ctggagcgtt ccggcacttt
     6181 tcatgcggtg gtgcagtcgc cctccagcgt tgcggcaacc tggcgtctgg ataccgaact
     6241 catccgtttg cagcaggatt tcaccgtgaa gccgagcgag gtgcgattca cgcttggggc
     6301 ccagttgatc gacatcgcga cgcagcgggt gatcgcatcg gcgcaattcg acgagagcga
     6361 gaagagcgac accgaggacg cctatggcgg cgtgctcgcc gccaaccggg cgttggagcg
     6421 gctgctggtc agactggcag aattctgcgc cgaaaatgcg cgctgaccga cttgcgccca
     6481 gtctgcagtg gcagcaattc ggggatggtt gagccgccac aaaatggaca ttgaggaatg
     6541 gcaggaatgt gccataaccc gttactggag attgataatt cactgacagt aatcggtcat
     6601 tggcgctaga tatttttcat taccgcattg cagtcagttg cccatgcgca tcatcgcttt
     6661 catcaccgat agcctcgatc aacgcaatct cgctgggaaa ttgcatggag ccatataggc
     6721 gtggtgtatg gacggctatt cgttctgcga aaaaatcgcc accgtgcatg gcccgatact
     6781 gactttccca gaacaggctc cccccgctgt gccgagtatc ttc
//
LOCUS       DASUIV010000191         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_696403, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000191 DASUIV010000000
VERSION     DASUIV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696403"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFW59_07015"
     CDS             <1..990
                     /locus_tag="VFW59_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364002.1"
                     /translation="RITIRSAVPLAHLRTRIHAPFEHLQSCGDIGMVMSSALDVRVED
                     SRNAYRDFHADWETRVADEARLLRELGADLVFSNVGYLPLAGAQQAGIPNAALCSLNW
                     SDIYRHYCGQDEIAAQIHSCYANADAFLRATPGMAMDAPSTGLRTGLTNLVPIEPIAA
                     IARNRRDELNRLLKLSTDEKLVLVSMGGIAGRLPIERWPRVEGVRYLVQAGWRVTHPD
                     VITFESVPVSFSELLASSDALLCKPGYGSFVEAACAGVPALYVNRPDWPETPALIDWL
                     QCHGASREVSRHALENGEITADLQALWHPPRPDRPIPSGGDQVANWLAERLGA"
     gene            complement(1006..>2004)
                     /locus_tag="VFW59_07020"
     CDS             complement(1006..>2004)
                     /locus_tag="VFW59_07020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5364003.1"
                     /translation="ILRLLLQKMADHPAAFTPITYALWYEFITNINPPLNKAVSELLS
                     AGEKLTDDVVCDLYQRHIADGNMAAEQAFKQNLQKVLDNLTQFAEVTDDEAGKFNDGL
                     RKHGEKLNQAVDAETLKHLIGEVAQDTQTMRSSVESLQSKLHESKSEVDRLRQELSSA
                     RSEALNDPLTGIFNRRGFEAQMRSLLANPEMQGKSVSFLMVDIDFFKKINDSYGHLFG
                     DKVIRIVAEVLKAQVKGQDSVARMGGEEFAVILPNTPLEGAQVVAEHIRKLVEKGRIR
                     RPDRDESVGGITISIGIAGCEIGTDWLTALAHSDEALYVSKQSGRNRTTIYSPPKG"
BASE COUNT          362 a          650 c          583 g          409 t
ORIGIN      
        1 cgcatcacca tacgctcggc ggtgccgctt gcccatctgc gcacgcgcat ccatgcgccg
       61 ttcgaacatc tgcaaagctg cggcgacatc ggcatggtaa tgtcctcggc gctggacgtg
      121 cgcgtcgagg atagccgtaa cgcttatcgc gattttcacg ccgactggga gacgcgtgtc
      181 gcagacgagg cccggctgct gcgcgaactc ggcgcagact tggtgttttc caacgtcggc
      241 tatctgccgc tagccggcgc gcagcaggcg ggcattccga atgcggctct gtgttcgctg
      301 aactggtcgg acatctaccg acattactgc ggccaagatg agatcgccgc ccaaatccat
      361 tcctgctacg ccaatgcgga tgcctttctc cgcgccacgc cgggcatggc gatggacgct
      421 ccttcgaccg ggctcaggac aggcttgacc aatctcgtcc caatcgagcc gattgccgca
      481 atcgcacgca atcggcgcga cgagttgaac cgccttctga aactatcgac cgatgagaag
      541 ctggtgctgg tgagcatggg cggcatcgca ggtcgcctgc cgatcgagcg ctggccgcgt
      601 gtagagggtg tgcgctatct ggtacaggca ggctggcggg tgacacatcc cgacgtcatc
      661 accttcgaat cagtgcccgt cagtttcagc gaactgctcg ccagcagcga tgcgctgctg
      721 tgcaagccgg gatacggcag ttttgtcgag gcggcctgcg cgggcgtgcc ggcactctat
      781 gtaaatcgtc cagactggcc ggaaacaccc gccctgatcg actggctgca atgtcacggc
      841 gcaagccgcg aagtctcgcg gcacgcattg gaaaacgggg agatcacagc agacttgcag
      901 gccttatggc accccccccg gccagaccgc cccattccat ccggaggcga tcaggttgca
      961 aactggctgg cagagcggct gggcgcgtga cgcccggcga aaccctcagc ctttgggcgg
     1021 gctatagatc gtggtgcgat tgcgcccgct ctgcttggaa acatagagcg cctcatcgga
     1081 atgagcgagc gccgtcagcc agtcggtacc gatctcgcaa ccggcgatgc cgatagagat
     1141 cgtgatgcca ccgacgctct cgtcccggtc cgggcggcgt atccgcccct tttcgacgag
     1201 cttccggata tgctcggcaa ccacttgagc cccctccagc ggcgtgttcg gcaggatcac
     1261 cgcaaactcc tcgccgccca tgcgggccac cgaatcctgg cccttaacct gcgccttcaa
     1321 cacctcggcg acgatgcgga tgaccttgtc gccgaacagg tgcccgtagc tatcgttgat
     1381 cttcttgaag aaatcgatat ccaccatcag gaagctgacc gacttgccct gcatctcggg
     1441 attggcgagc agacttctca tctgcgcctc gaatccgcgc cggttgaaga tccccgtcaa
     1501 aggatcgttc agcgcctcgc tgcgcgcact cgacaactcc tgacgcagcc tgtcgacctc
     1561 gctcttgctc tcgtgcaact tgctctgcag cgattcgacg gaagaacgca tggtttgcgt
     1621 gtcttgagcg acttcgccga tcaggtgctt cagcgtctcg gcatccactg cctggttcag
     1681 cttctcgcca tgcttgcgca gtccgtcgtt gaacttgcct gcctcgtcgt ccgtcacctc
     1741 ggcgaactgc gtcagattgt ccagcacctt ttgcaggttc tgtttgaacg cctgttccgc
     1801 cgccatattg ccgtccgcga tatggcgctg gtacaggtcg cacacgacat catcggtcag
     1861 cttctctccg gcactcagca actcgctcac cgccttgttc agcggtgggt tgatgttggt
     1921 gatgaactcg taccacaacg cgtaggtaat gggcgtgaag gcggccggat gatcggccat
     1981 cttctgaagc aacaacctca gtat
//
LOCUS       DASUIV010000192         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_417640, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000192 DASUIV010000000
VERSION     DASUIV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417640"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFW59_07025"
     CDS             complement(<1..781)
                     /locus_tag="VFW59_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase, dissimilatory-type subunit
                     alpha"
                     /protein_id="HEX5364004.1"
                     /translation="MAKKMHDTPNLDQLETGPWPSFVTGLKDLAKDKDYVVDLLGQLE
                     TSYKTRKGYWKGGTVGVFGYGGGVIPRFTELKNEDGTPKFKDAAEFHTLRIMPPAGMH
                     YDTGTLRQFCDLWEKHGSGLIAFHGQSGDIMFQGATTENVQKAFDELNEMGFDLGGAG
                     PAVRTSMSCVGAARCEQSCYDEAKTHRAVINTFLDDIHRPSLPYKFKFKFSGCPNDCM
                     NSVQRADMAVIGTWRDNMRTDEGLARKWFEKHGMNELVNDVI"
     gene            1083..1349
                     /locus_tag="VFW59_07030"
     CDS             1083..1349
                     /locus_tag="VFW59_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364005.1"
                     /translation="MAHFRARNKTNKRMHMADIKELTRFKVPLGSQEIELQQIDHAEG
                     GMSLLRIRIREGKRFTIFDIDPATAQHWAAAMQDWAKTQQPHDE"
     gene            1339..1959
                     /gene="cas6"
                     /locus_tag="VFW59_07035"
     CDS             1339..1959
                     /gene="cas6"
                     /locus_tag="VFW59_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HEX5364006.1"
                     /translation="MTSKPAPAAPEMIDLAFDISGNALPANHPFTLWEALINLAPALA
                     DADRAGILPMRLPEHAAATLPKRAKLTLRIPAALAEELTVGLSGEQLDLGGSLLQLGM
                     GRPRAITAYPTIHAHQVASNEDEPAFMEIVRAQLDEMRIACNLICGMRHRIGDETQSI
                     EGYSLVIHDLKADASLKLQYAGLGEARQYGCGIFVPYKVISGLNDD"
     gene            1996..2325
                     /locus_tag="VFW59_07040"
     CDS             1996..2325
                     /locus_tag="VFW59_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX5364007.1"
                     /translation="MGYVVAGNELETDDEGFLLEADYSDEVVAVIAAAENIELTPKHW
                     EVINYMREQYREHGHTPNFRNMLKGVNEFWAEADSKALYDLFPLGPAKQAAKIGGLPQ
                     PYGKGGY"
     gene            2507..2992
                     /locus_tag="VFW59_07045"
     CDS             2507..2992
                     /locus_tag="VFW59_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX5364008.1"
                     /translation="MNTKDLHANVTLDMRGLTCPAPLLGAKRVVNDLTNEQILLLVSD
                     CPGTRDDLFAWAEQTGNQVLKTEPATGGGTGYYVMKGKGETHEANVLLDIRGVVCPGP
                     IVEAKKLLNAMNSGEILKLISNCPGIHSDIVGWTSMTGIKLLESVEVASGEHAFYLQK
                     T"
     gene            2996..3607
                     /locus_tag="VFW59_07050"
     CDS             2996..3607
                     /locus_tag="VFW59_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364009.1"
                     /translation="MSRPNIRIKSNWFKAGREHTPEELAGAVSFVAWRIADNALKNTR
                     RADFEIAIGPQYFAYLVEFLIFNIQVADRIAYRRLSAEDRITFTGTLANRVADTLAEN
                     ESRLMGGAVSECKQRFIERLNRQADGYAEFDYDEQGPSFNFMRYLGYCMEPVVDEKDR
                     SWILDQTMSIEAPEAVEMLEKTLGNLLETEPRQARRRSGTSGD"
     gene            3611..>4187
                     /locus_tag="VFW59_07055"
     CDS             3611..>4187
                     /locus_tag="VFW59_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5364010.1"
                     /translation="MLTTPFDLEAGDAYLRWREHKLAQAITHIDELTVEVKNPAALTT
                     AEREALLSRCRRSNMAIYATRTAVDEQGVQRLGTQLGLLRLDANWLADDQGISRIAVS
                     NGDGPRQAYIPYTDRAIKWHTDGYYNPPERRIRGMILHCVQPAAQGGENRLLDHEITY
                     LMLRDADVEFVRALSAPDAMTIPERTDEHGVA"
BASE COUNT          923 a         1319 c         1183 g          762 t
ORIGIN      
        1 tgatcacgtc gttgaccagc tcgttcatgc catgcttttc gaaccacttg cgcgccagcc
       61 cttcgtcggt gcgcatgttg tcgcgccagg tgccgattac cgccatgtcg gcgcgttgca
      121 ccgagttcat gcagtcgttc gggcaaccgg agaacttgaa cttgaacttg taaggcagcg
      181 agggacggtg gatatcgtcc aggaaagtgt tgatcaccgc acgatgggtc ttcgcctcgt
      241 cgtagcagga ctgttcgcaa cgcgcggcgc ccacgcagga catcgaggta cgcaccgcag
      301 gacccgcgcc gccgagatcg aaacccatct cgttgagctc gtcgaacgcc ttctgcacgt
      361 tctcggtggt cgcgccctgg aacatgatgt cgcccgactg gccgtggaac gcgatcagac
      421 cggaaccgtg cttctcccac aggtcgcaga attgacgcag cgtgccggtg tcgtaatgca
      481 tgccggccgg cggcatgatg cgcagcgtgt ggaattcggc cgcatccttg aacttcggcg
      541 taccgtcctc gttcttcagt tcggtgaagc gcgggatcac accgccgccg taaccgaaca
      601 cgccgaccgt gccgcccttc cagtagccct tgcgggtttt gtacgaggtt tcgagctgac
      661 ccagcaggtc aaccacataa tccttatcct tggccagatc cttcaaaccg gtcacaaaac
      721 tgggccatgg gccagtctcg agctgatcga ggttgggggt atcatgcatc ttcttagcca
      781 taatcttcct ttcagtcgcg tcgcccgaaa ctcttactgc aggcaaacgt tgattgaccc
      841 ggcagaggcg ggatcctgta tctttaaggt ctgtattggc cgcgcttggt attattgttt
      901 cgcggcccgc tcgaaatttc gtacatctca ggcattcacc tttgacgaat actacggtgc
      961 atacctccgg cattccatta ccctgagcaa ctaccctcta taacccttaa gggtggtacg
     1021 cgaccttccc ccagtagtat ttaatgccgc aaggattggg gatagacttc atgcgtccgt
     1081 gaatggcgca cttccgcgca cgcaacaaga ccaacaaaag gatgcacatg gccgatatca
     1141 aggaattgac ccggttcaag gttccgctgg gcagccagga aatcgaattg cagcagatcg
     1201 accatgccga gggcggcatg agcctgttgc gcatccgcat ccgcgagggc aaacgcttca
     1261 ccattttcga catcgatccg gccacggcac aacactgggc cgccgccatg caggactggg
     1321 caaaaaccca gcagccacat gacgagtaaa cccgcaccgg cagccccgga gatgatcgat
     1381 ctggccttcg acatcagcgg gaacgcgctg cccgccaacc accccttcac gctctgggag
     1441 gcgctaatca acctcgcgcc cgcactggcc gacgcggatc gtgccggcat cctgccgatg
     1501 cgcctgcccg aacatgccgc agcaaccctg cccaaacgcg ccaagctgac cctgcgcatc
     1561 cccgcagcgc ttgccgaaga gctgacggta gggctatccg gcgagcaact cgatctgggg
     1621 ggcagcctgt tgcagctggg catgggcaga ccccgtgcga tcacggctta cccgaccatc
     1681 cacgcccatc aagtcgccag caacgaggat gaaccggcct tcatggagat cgtgcgcgcg
     1741 caactcgacg agatgcgcat cgcctgcaat ctgatctgcg gcatgcgcca ccgcatcggc
     1801 gacgagaccc agtccatcga aggatacagc ctggtcatcc atgacctgaa agccgatgcc
     1861 tcgctgaagc tgcaatacgc cggactaggc gaggcgcgcc agtacggctg tgggattttc
     1921 gtaccttaca aagtcatctc cggtttgaac gatgactgag tctgattttt ttacataacc
     1981 tagtggagaa aaacgatggg atacgtagtc gcagggaatg aactggaaac cgacgatgag
     2041 ggctttctgc tggaggcgga ctacagcgat gaggtcgtgg ccgtgatcgc cgccgccgag
     2101 aacatcgagc tgacgccgaa acactgggaa gtgatcaact acatgcgcga gcaataccgc
     2161 gaacacggcc acacgcccaa cttccgcaac atgctcaaag gcgtcaatga attctgggcc
     2221 gaggcagaca gcaaggcgct gtatgacctg ttcccgctcg gcccggccaa acaggccgca
     2281 aagataggcg gcctgcccca gccatacggc aagggcggct attgattcag ggaaaaccca
     2341 tgtccacgaa agacacgaaa agcacgaaag aaaagctcaa tctggtttga acccgacgac
     2401 tcactgatga gcacgggttg tttttatttt tcgtgtgttt cgtgtctttc gtggactagc
     2461 tttcgttttt aactaaccag cttcagcaac aaacaggaca accgacatga acacaaaaga
     2521 cttgcacgcc aacgtaacgc tggatatgcg cggcctgacc tgccccgctc cgctgctggg
     2581 cgccaaacgc gtggtcaacg atctgaccaa cgaacagata ctgctgctgg tctcggactg
     2641 cccgggcacg cgcgacgatc tgttcgcctg ggcggaacag accggcaatc aggtactgaa
     2701 gaccgaaccg gcgaccggtg gcggcaccgg ttactacgtc atgaagggca agggcgagac
     2761 gcatgaagcc aacgtgctgc tggacatacg cggcgtggtt tgcccgggcc cgatcgtcga
     2821 agccaaaaaa ctgctgaacg cgatgaattc cggcgagatc ctgaaactga tcagcaactg
     2881 ccccggcatt cattccgaca tcgtcggctg gaccagcatg accggcatca aactgctgga
     2941 gtccgtcgag gtggcttcgg gagaacacgc cttctatctg cagaagacct gaatcttgtc
     3001 gcgccccaac atccgcatca agagcaactg gttcaaggcc ggtcgcgaac acacgccgga
     3061 ggaactcgcc ggcgcggtct cgttcgtcgc ctggcgcatc gccgacaatg cgctgaagaa
     3121 cacccgccgt gccgatttcg agatcgccat cggcccgcag tatttcgcct atctggtcga
     3181 attcctgata ttcaacatcc aggtggcaga ccgcatcgcc taccgccgcc tgtccgccga
     3241 ggatcgcatc acattcaccg gcacgctggc aaatcgcgtg gccgacacct tggccgagaa
     3301 cgagagtcgg ctcatgggag gagcggtgag tgagtgcaag caacgcttca tcgaacgtct
     3361 caaccggcag gccgacggct atgccgagtt cgattacgac gagcaagggc cgtcgttcaa
     3421 cttcatgcgc tacctcggtt actgcatgga gccggtcgtg gacgagaagg accgcagctg
     3481 gatactcgac cagaccatga gcatcgaggc gcccgaggcc gtcgagatgc tggaaaagac
     3541 cctgggcaat ctgctcgaaa ccgaaccgcg tcaggctcgc cgacgcagcg gaacaagcgg
     3601 cgactgatcc atgctcacca ccccgttcga tctcgaagcc ggtgacgctt acctgcgctg
     3661 gcgcgagcac aagctggcgc aggccatcac ccatatcgat gagctgaccg tcgaagtaaa
     3721 gaaccctgcc gcgctgacga ccgccgaacg cgaggcgctg ttatcgcgtt gccgtcgcag
     3781 caacatggca atctacgcca cccggaccgc cgtggatgag caaggggtgc aacggctcgg
     3841 cacgcaactc ggcctgctgc ggctggatgc caactggctg gccgacgatc agggcatctc
     3901 gcgcatcgcc gtcagcaatg gcgacggacc gcgtcaggct tacatcccct acacagaccg
     3961 cgccatcaaa tggcataccg acggctatta caacccgccc gaacgccgga tacgcggcat
     4021 gatcctgcat tgcgtccagc ccgccgcaca gggcggcgag aatcgtctgc tggatcacga
     4081 gatcacctac ctgatgctgc gagacgccga tgtcgaattc gtccgcgccc tctctgcccc
     4141 ggacgcgatg accatccccg aacgcaccga tgaacacggc gtcgcac
//
LOCUS       DASUIV010000193        13913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1161994, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000193 DASUIV010000000
VERSION     DASUIV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 13913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13913
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161994"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFW59_07060"
     CDS             <1..378
                     /locus_tag="VFW59_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5364011.1"
                     /translation="GPLAGLAAALASIETPWLFAVACDMPFVAPALLEYLAGRRAGYQ
                     AVVPVVQGCPQPLAAFYAGSCLESVREILAGDGKHSLRALLERLDVCYVDEAQLLEHD
                     PQLRSFFDLDTPEDVAALSNGVA"
     gene            378..1631
                     /gene="glp"
                     /locus_tag="VFW59_07065"
     CDS             378..1631
                     /gene="glp"
                     /locus_tag="VFW59_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5364012.1"
                     /translation="MEYLSVSVAQRCVLDSVMTLGVETVRLEQSLGRVLAEDIRANRD
                     LPPYDVSAMDGFALRSADVAVGPVMLEVVEDIKAGDMPTRVVQAGQCARIMTGAPVPQ
                     GADAVIRVEDSEAQTDGRVRFGKAAPAGNDIRLRGEGMRSGDIVLPAGTEITPGVAGV
                     LATVKQAQFQVYRRPRVAILSTGNELEGMDEPLDPNKIPDANSYALMAQVQALGIEPV
                     LLGIARDDPVELERYLRQGLEYDVLLVSGGTSVGVHDYVRPTVEALGVQMKFWRVAMR
                     PGHPLAFGVMGRPNNTQAMVFGLPGNPVSSMVCFEEFVALALRGMMGHARLHRRTVSA
                     RLAHPVKMRPGRTEFVRVVLSRDEQGYVATSTGTQSSGALLSMAKADGLLVVPSDSSG
                     LVANEVVTVQLLDGTAFQDETGFRE"
     gene            1634..2179
                     /gene="mog"
                     /locus_tag="VFW59_07070"
     CDS             1634..2179
                     /gene="mog"
                     /locus_tag="VFW59_07070"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEX5364013.1"
                     /translation="MSIARIGILTISDRASRGEYEDKGGPAMHAWFTRVLSSPWEAVA
                     RVIPDEQPLIEAALRELSDVAGCSLIVTTGGTGPALRDVTPEATEAVCEKMMPGFGEL
                     MRTASLKFVPTAILSRQTAGIRGKSLIVNLPGKPSAIDDCLNAVFPAIPYCIDLIGGA
                     YLESDPEQCKAFRPAHAKPKI"
     gene            complement(2202..3764)
                     /locus_tag="VFW59_07075"
     CDS             complement(2202..3764)
                     /locus_tag="VFW59_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEX5364014.1"
                     /translation="MDKDEKLRAISQLVFELTVASSTTSDLDKLLERLFGMLCTYREL
                     SLQPRGAVVLLNPRGRYFQVAQFGIDPIWKTDFRWTSGSFAAAEIAQECAMETISFPT
                     DSANNPSDATQHTLLLPLRIEDQGVGYAVLFTDSDYQPSAVHLDFMSDLARALSGLVH
                     RSLSNETLRIRELELEEARADALRSLGVASEYRDNETGWHIMRMTNFAQAIAKAMDMP
                     TAQRELLYVAAPMHDVGKIGISDAVLLKPGKLTPAEFETMKTHTQIGVTILTGNDSLI
                     AAAREIAGSHHERWDGTGYPGGLAGEQIPILARICSVADVFDALTSKRPYKQPWTIEE
                     ARDWVIAESGRHFDPAVVSAFELAMPEILRIRELYRDDIIDPKQVLTLPPLAPREGAW
                     VSWDDLLSVGIDIIDEHHRYLFDLINDLFDVVENKRGARQVARLIKSLDAYAKVHFRA
                     EEQMMTHYAFEGISRQQHQHHAFEAKIAEFYEEMHANPLVAQFDVLTYLRDWLIHHIR
                     VEDAQLRSLVSA"
     gene            complement(3837..5369)
                     /locus_tag="VFW59_07080"
     CDS             complement(3837..5369)
                     /locus_tag="VFW59_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5364015.1"
                     /translation="MTPVSAAPLIQALLTCPDCYDHPVTRIELIETHISWVLLTGEYA
                     YKIKKPVNLGFLNFSTVALRRQDCEDEVRLNRRLAPACYLGVIPISGSPDMPRLNGPG
                     EPIEYAVKMRQFPPEATLDQLAERGELGIEQIDRLAARLAHFHLSECEPAPAGSPWGE
                     PDTIAGPVEENFKLLMEKPGSAQQLQRIQDWSITEHRRLEPLMRERKRSGRVRECHGD
                     LHLGNLAWIDGELLIFDCLEFNAALRWIDIISEVAFCYMDLLHRQQPELAARFLNAWL
                     EITGDYDGVALLRYYAVYRAMVRAKVAALRGVQIVEESETTANRAELDSCLQLAERLS
                     QCEPPQLWITHGLSGSGKTTLTQSLLQSRSMIRLRSDVERKRLAGLDALARSGEDIGA
                     GLYSQSASRNTYLQLASLAAHLLKAGWPVVVDAACLERWQRDLFHDLAHRLGATFRIL
                     DIQADPATLRERIAKRTEQGRDASDASMNVLQHQIETREPLAPSELANVTVIAQGELP
                     KL"
     gene            complement(5366..6232)
                     /gene="rsmI"
                     /locus_tag="VFW59_07085"
     CDS             complement(5366..6232)
                     /gene="rsmI"
                     /locus_tag="VFW59_07085"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5364016.1"
                     /translation="MHQLHPQKLEAALYVVATPIGNLRDITLRAIDVLSAADVVAAED
                     TRNTAHLLSHHGISAQRLMAVHQHNERGAADKIVALLQSGQSVAFVSDAGTPAVSDPG
                     ALLVQAVLAAGLRVIPIPGASAAIAALSASGLAAPHFLFYGFLPNKSAARCSALQTLN
                     EHPYTLVFYEAPHRIVECVVDLNTVLGGERQIVLAREVTKLFETIHACRLNEAEAWLL
                     SDSNQQRGEFVVLVSGAEPPSGLSAETLATLTLLLDELPLKQAVQLAAKISGANRSEL
                     YQLALQMKADSE"
     gene            6272..7357
                     /locus_tag="VFW59_07090"
     CDS             6272..7357
                     /locus_tag="VFW59_07090"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX5364017.1"
                     /translation="MSSFAYAIEEPIPAASLPLNNEKPLPHIALLLPLNAELFAHSAE
                     IVRQGFMAAAGTELRSLPVRVYSSSDESSEVVALYRQAVASGARAIVGPLTRSGVVAL
                     AAEQNIPVPTLALNVIEEKPAGPLYFFGMAAESEARQVARQAAQRGFKQAIAISSQTQ
                     LSKRLLFAFEDEWHKLGGATLSEIEFNGDPTPLLDMQSGPDVMVFLSVDAANAQLIRP
                     YLPNRLATYATSQVFVGNQDTLTNYELNDISFVDMPWLLQMDHPAVMIYPRAVPPLSA
                     DQERLYALGIDAFRLIQLMLTRQLGRAMPLDGVTGRIQLYGYTFERTSIPAVFGQGRA
                     LLPDTPAAAIAPMFPEQARKQAETVQQ"
     gene            7354..7698
                     /locus_tag="VFW59_07095"
     CDS             7354..7698
                     /locus_tag="VFW59_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX5364018.1"
                     /translation="MNERGLQAERWAAQYLQQQGLQLVAQNYRGRFGEIDLIMRDGAT
                     LVFVEVRLRRNADFGGAAASIDAHKQRRIIRTAQQYLATLGRIPPCRFDALLLGDAPE
                     QGVQWLKNAFEA"
     gene            7738..8325
                     /locus_tag="VFW59_07100"
     CDS             7738..8325
                     /locus_tag="VFW59_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEX5364019.1"
                     /translation="MNINKRIIKHFEDSAELKLRIKDALAEPIANGAQLFFDCVTNDG
                     KILCCGNGGSVADAQHFAAELLNRFEKERPGLAAVALTTDSSVLTSIANDYDFNQVFS
                     KQVRALGLPGDSLLAISTSGNSTNVVAAIRAAHERSMRVVALTGRDGGEMAAILRPGD
                     VLICVETKSTARIQEVHLLALHCLCDIMDYLLLGE"
     gene            8334..8897
                     /locus_tag="VFW59_07105"
     CDS             8334..8897
                     /locus_tag="VFW59_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5364020.1"
                     /translation="MVSLLLLALLAGSLQGCFPVIAAGVGTGVSMAQDRRTSGAYIED
                     EAIEDKAVDRIGKRYKDGVHVNVTSFNRTVLVTGEVLSESAKAEIGKLVGGIENVRNV
                     TNELAVSGVSSLTSRSSDSLVTSNVKIRFMRDKRFNAEHVKVVTENGTVYLMGIVTRA
                     EADMATEIASTTSGVMRVTRLFEYLGN"
     gene            8897..9382
                     /gene="rfaE2"
                     /locus_tag="VFW59_07110"
     CDS             8897..9382
                     /gene="rfaE2"
                     /locus_tag="VFW59_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX5364021.1"
                     /translation="MYPAPAFEAKLCAADELAARVAVLPRPLVFTNGCFDVLHRGHVT
                     YLAQARALGASLIVGVNSDASVKRLGKGDDRPINRQDDRMAVLAALEAVSLVVPFDED
                     TPLNLILACKPDVLVKGGDWQPESIVGSKEVLGWGGKVHSVPFLHERSTTALLKKIRS
                     L"
     gene            9385..9741
                     /locus_tag="VFW59_07115"
     CDS             9385..9741
                     /locus_tag="VFW59_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX5364022.1"
                     /translation="MTPTEITLHQKSKTLEIAFDDGARYTLPDEFLRVYSPSAEVRGH
                     GEGQEVLQVGKKDVGIVEVAPAGSYALKITFDDGHDSGLYTWEYLYGLGRDQEILWHN
                     YLHRLEAAGATREAAK"
     gene            9803..10534
                     /gene="ubiE"
                     /locus_tag="VFW59_07120"
     CDS             9803..10534
                     /gene="ubiE"
                     /locus_tag="VFW59_07120"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX5364023.1"
                     /translation="MADTHFGFETVAEEEKAKRVAGVFTSVASKYDVMNDLMSVGLHR
                     LWKRFAIGVSGVRSGQRVLDVAGGSADLSRLFLKQVGSSGQVVLTDINNAMLRVGRDR
                     LLDEGITTPVTQCDAEHLPFPDNYFDCVSIAFGLRNVTHKDAALREMKRVLKPGGRVI
                     VLEFSKVAKPLEKAYDFYSFKLLPKMGQLIANDADSYRYLAESIRMHPGQEELKQMMV
                     DAGLERVEYFNLTGGVVAVHRGYKF"
     gene            10601..11536
                     /locus_tag="VFW59_07125"
     CDS             10601..11536
                     /locus_tag="VFW59_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5364024.1"
                     /translation="MSQIPQNVERLAVLFADISGSTALYESRGDEVARRLIAQCITVM
                     VREVHAYKGTLIKTIGDEIMCVFPTAEEAFLSSCAMQNAVEKGRFEGGYPMHIRVGFH
                     YGDVIREASDVFGDTVNVAARVAAITRASQIMTTQAAVDALSPAAREKTRQILRAEFK
                     GKQEQFDISLVIWEMDDMMSTRIGTPVFRKTPDNIDELLLRYHDQTLKVNKERRSVVL
                     GRGETCDLVVQNNFASRQHLKIELRFGKFVAVDQSTNGSYVRFADGHVMRIAREEINL
                     LGKGSISMGQPYSDNDGELIEFSINSPHAAGKNTA"
     gene            11626..11967
                     /locus_tag="VFW59_07130"
     CDS             11626..11967
                     /locus_tag="VFW59_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HEX5364025.1"
                     /translation="MSALPKCPKCSSEFTYEDGSNYVCPECAHEWSKDAAVEGGEQQR
                     VYKDAFGNVLADGDSVTVIKDLKIKGSSSVVKVGTKVKNIRLVDGDHDIDCKIDGIGA
                     MQLKTEFVKKA"
     gene            11989..12615
                     /locus_tag="VFW59_07135"
     CDS             11989..12615
                     /locus_tag="VFW59_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5364026.1"
                     /translation="MPAADLELLYSDASLLVVNKPAGLLAVPGRGADKQDCLSARIQQ
                     EFADALVVHRLDMATSGLMVFARGTGMQSDLSRLFRERLVEKRYIACIAGQISPATGE
                     IALPLGADWPNRPRQKVDADTGKPSLTRYRLLDYDDDSDISRIELEPVTGRTHQLRVH
                     MMAAGHAIVGDRLYGGREASRLMLHARSLSFAHPVGGEPLALASEPPF"
     gene            12672..13508
                     /locus_tag="VFW59_07140"
     CDS             12672..13508
                     /locus_tag="VFW59_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HEX5364027.1"
                     /translation="MKRFLMLLTIAMTCLSLFAATAEAKRFGGGSSFGKQRSMSPQQA
                     QKAPAAAPAPAPGAAPAKPGNKWLGPLAGLAIGAGLGMLFANMGMGGAMGGILMALLA
                     GVAVMFLISMFRKKQPQPAMQYAGAGAPYGSSQPEQPGGGSAVAAPEARSIPADFPVD
                     SFLRSAKTSFIRLQAANDRKDLNDVREYTTPEMFAEVSMQMRERGDAPQKTDVVAIQA
                     DLLEVANEGNLAIASVRFTGQLCENNGAPESIDEIWHVQKDLSDDKSVWLLAGIQQTT
                     LH"
     gene            13539..>13913
                     /locus_tag="VFW59_07145"
     CDS             13539..>13913
                     /locus_tag="VFW59_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364028.1"
                     /translation="MLNHLLLQNRWAMSRLARFAGKTARFNIAPFSLVWTIQPDGSLQ
                     GADAAISADAVCDIPFSLLPRLAMHDERAHAEIRSEGDAALLTEIFFLSRNLHWDAAE
                     DLSHVTGDVAAERIVQAAQDTKQ"
BASE COUNT         2732 a         4007 c         4349 g         2825 t
ORIGIN      
        1 gggccgctag ccgggctggc tgccgcgctg gcaagtatcg agaccccctg gctgtttgcg
       61 gtcgcttgcg acatgccgtt cgtcgcgccc gccttgcttg aatatctggc tgggcgacgg
      121 gccgggtatc aggccgttgt gcctgtggtg cagggctgtc cgcaaccgct ggccgcgttc
      181 tacgccggca gctgtctcga gtcggtacgg gaaattctgg cgggtgacgg caagcacagt
      241 ctgcgcgcgc tgttggaacg cctggatgtt tgttatgtgg atgaggcgca gctgctggaa
      301 cacgatccgc agttgcgcag tttttttgat ctggatacgc cggaggatgt cgcggcgcta
      361 tcgaatggag tagcttgatg gaatatttgt cggtaagtgt ggcgcaacgc tgcgtgctgg
      421 attcggtcat gacgctgggc gtcgagacgg tcaggctgga gcagtcgctg gggcgagtgc
      481 tggccgaaga cattcgcgcc aatcgcgatc tgccgcccta cgatgtctcc gcgatggacg
      541 ggttcgcgct gcgcagcgcc gatgtggccg ttggtccggt catgttggag gtcgttgagg
      601 atatcaaggc cggcgatatg ccgaccaggg tcgtgcaggc cggacaatgt gcccgcatca
      661 tgactggcgc cccggttcca caaggggcgg atgcggtgat acgggtggaa gacagcgagg
      721 ctcaaacaga cggacgggtg cgcttcggca aggccgcacc ggccggcaat gacatccgcc
      781 tgcgcggcga ggggatgcgt agcggtgaca tcgtgttgcc ggcaggtact gaaattaccc
      841 ccggcgtcgc tggagtgctg gcgacggtga agcaggctca gtttcaggtc tatcgtcgcc
      901 cgcgcgtggc cattctgtcc accggcaatg aactggaagg catggacgaa ccgctcgatc
      961 cgaacaagat tccggacgcc aacagctacg cgctgatggc gcaggtgcag gcgctgggta
     1021 ttgagcctgt gctgctcggg attgcacgcg atgatcccgt cgaactggag cgctatttgc
     1081 ggcaggggct ggaatatgac gtgttgctgg tttccggcgg cacgtcggtc ggggtacacg
     1141 actacgtccg tccgaccgtc gaggcgctcg gtgtgcagat gaaattctgg cgcgtagcga
     1201 tgcgtcccgg acatccgctg gcattcggtg tgatggggcg tccgaacaac acgcaggcta
     1261 tggtatttgg gctgccgggc aatccggtgt ccagcatggt gtgcttcgag gaattcgtcg
     1321 ctctcgcatt gcgcggcatg atgggtcatg cgcggctgca ccgacgtacc gtgagcgcac
     1381 gactcgcgca tccggtgaag atgcgtccgg ggcgcaccga gttcgtgcgt gtcgtattga
     1441 gccgcgatga acagggctat gtggcgactt cgaccggcac tcagagcagc ggcgcattgc
     1501 tgtccatggc taaagccgac ggactgctgg ttgttccatc agatagcagc gggctggtgg
     1561 cgaatgaggt ggtgacggtg cagttgctgg atggcacggc atttcaagac gaaacgggtt
     1621 tcagggagtg agcatgagca ttgcgcggat cggaattctg acgatatcgg atcgcgccag
     1681 tcgcggtgaa tatgaggata aaggcgggcc agccatgcac gcctggttca ctcgcgtgtt
     1741 gagttcgccc tgggaggcgg tggcgcgggt gattcccgac gagcagcctc tgatcgaggc
     1801 ggcgctacgc gaactgagcg atgtggccgg ttgcagtctg atcgtcacga ccggcggaac
     1861 cggcccggcg ctacgcgatg tgacgccgga agcgactgag gcggtatgcg agaagatgat
     1921 gccgggtttc ggtgagctga tgcgcaccgc ttcgttgaag ttcgtgccta ccgcgatcct
     1981 gtcgcgccag accgcaggga tacgtggcaa gagcctgatc gtcaatctgc cgggcaagcc
     2041 ctcggccatc gatgactgcc tgaacgcggt gttcccggcg attccctatt gcatcgatct
     2101 gatcgggggc gcgtatctgg aatccgaccc ggagcaatgc aaggcctttc gtcccgcgca
     2161 cgcgaaacca aagatttaat ccccgccgct ggctaggccg gtcaggcgct gaccagcgaa
     2221 cgcaactggg cgtcctcaac ccggatgtga tgaatcagcc aatcgcgcaa ataggtcagc
     2281 acatcgaatt gggcaaccaa tgggttggca tgcatctctt cgtagaattc ggcgatcttg
     2341 gcctcgaaag catgatgttg atgctgctgg cgcgagatgc cctcgaacgc atagtgcgtc
     2401 atcatctgtt cttccgcgcg gaaatgcact ttggcgtagg cgtccagcga cttgatcaat
     2461 ctcgcgactt gacgtgcccc ccgcttgttc tcgaccacat caaacaagtc gttgatcaga
     2521 tcgaacaggt agcggtgatg ttcgtcgatg atgtcgatac ccacgctgag aagatcatcc
     2581 cagctcaccc aggcgccttc tctcggtgca agcggaggca gtgtcagaac ctgcttcgga
     2641 tcgataatgt cgtcgcgata taactcgcgt atgcgcagga tctcgggcat ggccaattcg
     2701 aaggcgctga ctacggcagg atcgaaatgc ctgcccgatt cggcgatcac ccagtcgcgc
     2761 gcctcctcga tcgtccatgg ctgcttgtaa gggcgcttcg atgtcagcgc gtcaaacacg
     2821 tcggctacag agcaaatgcg agccagaatc ggtatctgtt cgccagcgag ccctcccgga
     2881 tagccggttc catcccagcg ttcatgatgc gagcccgcaa tttcccgggc ggcggcgatc
     2941 agcgaatcat tgcccgtcag gatggtgacc ccgatctggg tgtgggtctt catggtttcg
     3001 aactctgccg gggtcagctt gcccggtttc agcaagaccg catccgaaat gccgatcttc
     3061 ccgacatcgt gcatgggggc cgcgacgtac aacaactcgc gttgtgccgt cggcatatcc
     3121 atggccttgg ctatcgcctg tgcaaagttg gtcatgcgca tgatgtgcca accggtttcg
     3181 ttgtcgcgat attcggaggc gacgccaaga ctgcgcaggg cgtcggcgcg cgcctcctcg
     3241 agttccagtt cgcgtatgcg taaggtctcg ttggacaggg agcgatgaac cagaccggac
     3301 agcgcccgtg ccaagtcgct catgaagtcc agatgtaccg ccgagggttg atagtcagaa
     3361 tcggtaaaca gaacggcgta acccacaccc tgatcttcga tgcgcagcgg caataacaag
     3421 gtatgttgcg tcgcgtcact cgggttgttt gcggaatcag ttggaaagga gatcgtttcc
     3481 atcgcgcatt cctgcgcgat ttcggcggcg gcaaacgagc cggatgtcca gcggaagtcg
     3541 gtcttccaga tcggatctat accaaactgg gctacttgaa agtagcggcc gcgcggattg
     3601 agcaggacga cggcgcctcg cggctgcaag gacaattcgc gataggtgca taacatgccg
     3661 aataatcgtt ccagcaactt gtccagatcg gaagtcgtgc tgctggcaac ggtcagctca
     3721 aataccagct ggctgatcgc acgtaatttt tcgtctttgt ccacaaagtc cctcccctaa
     3781 ccactgcttt tttattattg gcgaaacata acaggagacg tgaaaaataa ccacaactat
     3841 agttttggca gttctccttg agcaatcacg gtgacattgg ccagttcgga tggggccaga
     3901 ggctcgcggg tttcaatctg atgctgcagg acgttcatgc tcgcgtccga ggcgtccctg
     3961 ccctgctcgg tgcgtttggc gatacgctcg cgtagcgtgg cggggtcggc ttgaatatcc
     4021 aatatccgaa aggttgcccc gaggcgatgt gcgaggtcgt ggaacaggtc gcgctgccat
     4081 cgctcaagac aggcggcgtc gacgacaacc ggccaccccg ctttcagcag atgcgccgcc
     4141 agagaagcca gctgcaggta ggtgttgcgg ctggctgact gcgaatacag gcctgcgcct
     4201 atatcctccc cgctgcgagc cagcgcatcc agtccggcaa gacgcttgcg ttcgacatcg
     4261 gagcgcaggc ggatcatgct ccggctctgc aagagcgact gggtcaaggt ggtcttgccg
     4321 gaaccggaca ggccgtgggt gatccacagt tgtggcggtt cacattgcga cagccgttcg
     4381 gccagctgca aacaggagtc gagctcggcg cgattggcag tcgtctcgct ctcttccact
     4441 atttgtacgc cacgcaatgc cgcgaccttg gcgcgcacca tggcgcgata caccgcgtaa
     4501 tagcgcagca gcgccacacc atcatagtcg ccggtgattt ccagccaggc attgaggaag
     4561 cgcgcagcca gttcgggctg ctgacgatgc agcagatcca tgtaacagaa tgcgacttcc
     4621 gagatgatgt cgatccagcg caaagccgcg ttgaattcga ggcagtcgaa gatcaggagc
     4681 tcgccgtcga tccatgccag attgcccaag tgcagatcgc cgtgacattc gcgcacccgg
     4741 ccgctgcgct tgcgttcgcg catcagcggt tccagtcgcc gatgttccgt aatgctccag
     4801 tcttggatgc gttgcagctg ttgggcgctt cccggcttct ccatcaataa cttgaagttt
     4861 tcttcaaccg gtccggcgat ggtgtctggt tcgccccatg ggcttccggc gggagccggt
     4921 tcacattcgc tgagatgaaa atgggccagt cgtgcggcaa ggcggtcgat ttgttcgata
     4981 cccagttctc cgcgttccgc caactgatcc agcgtggcct ccggcgggaa ctggcgcatc
     5041 ttcaccgcgt attcgatcgg ttcacccggg ccgttgaggc gcggcatatc cgggctgccg
     5101 cttataggga tcacgccgag atagcaagcc ggagcaagcc ggcggttcag cctgacttcg
     5161 tcctcgcagt cctggcgccg caacgccaca gtgctgaaat tgaggaaacc cagattgacg
     5221 ggcttcttga tcttgtaggc atattctccg gtgagcagca cccaggatat atgggtctcg
     5281 atcaattcta tccgcgtgac cggatggtcg tagcaatctg ggcaggtcag cagtgcctga
     5341 atcaatggcg cggctgacac gggcgtcatt ccgaatcggc tttcatctgg agcgccagct
     5401 gatatagctc gctgcggttt gcgccgctga tcttggcggc cagttgcacc gcctgcttga
     5461 gcggtagttc gtcgagcagc agggtcagtg tggccagggt ctctgccgac aagcccgacg
     5521 gtggctccgc cccggagacc agtacgacga attcgccacg ctgctggttg ctgtccgaca
     5581 gcaaccaggc ttcggcctcg tttaggcggc aggcatggat ggtctcgaac agtttggtga
     5641 cctcgcgcgc cagtacaatc tggcgttcgc cgcccagtac ggtattgagg tcgacaacgc
     5701 attcgacgat gcgatgcggt gcctcgtaga acaccagcgt gtagggatgt tcgttcagcg
     5761 tttgcagcgc actgcagcga gcggcggatt tgttgggcag gaagccgtag aacagaaagt
     5821 gcggtgcagc caggccggag gccgacagcg cggcgatcgc agcgctggca cccggtatcg
     5881 ggatgacgcg cagccccgcc gccagcacgg cctgcaccag caaagcgccg gggtcgctga
     5941 cggcgggcgt gcccgcgtcg ctgacgaagg cgacgctctg accggattgc aataaggcaa
     6001 cgatcttgtc cgccgctccc cgttcattat gctgatgcac agccatcaat cgctgcgcgg
     6061 aaatgccgtg gtgcgaaagc aggtgcgcgg tgttgcgcgt gtcctcggcc gcgaccacgt
     6121 ctgcggcgga tagcacatcg attgcgcgca gggtgatatc gcgcaagttt cctatcggag
     6181 tggcaactac atataatgcg gcttccagtt tttgcggatg taattgatgc aacgcctttt
     6241 cctcttgctt ctgactttgt tcgcactata cgtgagcagt tttgcttacg ccatcgaaga
     6301 gccgataccc gcagcctccc tgccgctcaa caacgagaag ccgctgccgc acattgcact
     6361 gctgctgcca ttgaatgcgg agctcttcgc gcattctgcc gagatcgtgc ggcaaggctt
     6421 tatggcggca gccggaacgg aactgcgttc gttgccggtg cgtgtataca gcagttccga
     6481 cgaaagcagc gaagtcgttg cgctgtatcg tcaggctgtc gccagcggcg cgcgtgccat
     6541 agtcggcccg ttaacgcgca gcggcgttgt tgcgctcgca gcggaacaaa atattccggt
     6601 gccgacgctt gcgctgaatg tgatcgagga gaagcctgcg ggccccctct atttcttcgg
     6661 tatggccgcc gagtcggaag ccaggcaggt cgcccgacag gctgcgcaac gcggcttcaa
     6721 gcaggccatt gccatttcca gccagacgca attgtccaag cgcctgctgt ttgcgttcga
     6781 agatgagtgg cataagctgg gcggggcgac gctgagtgag atcgagttta acggcgaccc
     6841 gaccccgctg ctcgacatgc agtcggggcc ggatgtcatg gtgtttctgt ccgtggatgc
     6901 agccaatgca caattgatcc gcccctatct acccaacagg ctggcgacct atgccacttc
     6961 gcaggtgttc gtaggcaatc aggatacgtt gaccaattac gaactgaacg acatcagttt
     7021 cgtcgacatg ccctggttgc tgcaaatgga tcacccggca gtgatgatct atccgcgcgc
     7081 cgtcccgccg ttgtcggccg atcaggagcg tctgtatgca ttgggcatcg atgcgtttcg
     7141 cctgattcag ctgatgttga cccgtcagtt aggccgcgct atgccgctgg atggcgtgac
     7201 cgggcgcatc cagttgtacg gctatacctt cgagcgcact tccattccag ccgttttcgg
     7261 acaggggcgt gccctgctcc cggatacgcc tgctgccgcc atcgcgccga tgttcccgga
     7321 acaggccaga aagcaggcgg agacggttca gcaatgaatg agcgcgggct gcaggccgaa
     7381 cgctgggccg cgcagtactt gcaacaacag gggctgcaac tggttgcgca gaattatcgt
     7441 ggtcgcttcg gcgagatcga cctgatcatg cgggatggcg cgacgctggt attcgtcgaa
     7501 gtccgcttgc gtcgcaatgc ggatttcggc ggggcggcgg ccagcatcga cgcccataaa
     7561 caacggcgca tcatccgcac ggcgcaacaa tacctcgcca ccctcggccg tatcccgccc
     7621 tgccgcttcg atgcgctgct gctgggtgac gcgccggaac agggcgtgca gtggctcaag
     7681 aatgcgtttg aagcctgatt cggttaatat tcgcgctgat caaacgagct gcctgccatg
     7741 aatatcaaca aacgtatcat caaacacttc gaagatagcg ccgaactcaa gctgaggatc
     7801 aaggatgcac tggccgaacc catcgccaat ggcgcccaac tctttttcga ctgcgtgacc
     7861 aacgatggca agattctatg ctgcggcaat ggcggttcgg tcgccgatgc ccagcacttt
     7921 gccgccgaat tgctgaaccg cttcgaaaag gagcgcccgg gcctggcggc agtcgcgctg
     7981 actaccgaca gctcggtgct gacctccatc gccaacgatt atgatttcaa tcaggtgttc
     8041 tccaagcagg tgcgggcgct cggcctgccc ggcgacagcc tgttggcgat ctccaccagc
     8101 ggcaattcga cgaatgtcgt ggcggcaatt cgcgccgccc atgaacgcag catgcgcgtg
     8161 gtcgccctga ccggacgcga tggcggcgaa atggccgcta ttctgcgccc gggcgatgta
     8221 ttgatctgcg tggagaccaa gagcacggca cgcatccagg aggtgcacct gctggccctg
     8281 cactgtctgt gcgacataat ggattacctg ttgttgggag aatgatgatg cgtatggttt
     8341 ccctgttgtt gctggctttg ttggcggggt cgctgcaagg ttgtttcccg gtgatcgccg
     8401 caggtgtcgg taccggtgtt tcgatggcgc aggacaggcg caccagtggc gcctatatcg
     8461 aggatgaggc tatcgaggac aaggccgtcg atcgaatcgg caagcgctat aaagacggtg
     8521 tgcacgtgaa tgtcaccagc ttcaaccgca ctgtgctggt caccggcgag gtgctcagcg
     8581 agagcgcaaa ggccgagatc ggcaagctgg tgggcggcat cgagaatgtg cgcaatgtga
     8641 ccaacgaact tgccgtctcc ggtgtcagct cgctgacttc gcgcagcagc gatagtctgg
     8701 tcacttccaa tgtgaaaatc cgcttcatgc gggacaaacg tttcaatgcc gagcacgtca
     8761 aggtggtcac cgaaaacggt acggtctacc tgatgggtat cgttacccgt gcggaagccg
     8821 atatggccac cgaaatcgcc agcaccacca gcggcgtgat gcgcgtgacg cggctgttcg
     8881 aatacctcgg caattgatgt atcccgctcc cgctttcgaa gccaagctgt gcgccgctga
     8941 cgaactcgcc gcgcgggtcg ccgttttgcc gcgtccgctg gtattcacca acggctgctt
     9001 cgacgtgctg catcgcgggc atgtcaccta tctcgcacaa gcccgcgccc tcggcgcctc
     9061 gctgatcgtc ggtgtgaaca gcgatgcgtc ggtgaagcgt ttgggcaagg gggatgaccg
     9121 cccgatcaat cggcaggacg accgcatggc ggtgctcgcc gcactggaag cggtcagtct
     9181 ggtggtgccc ttcgacgaag atacgccgct gaatctgatc ctcgcctgca aaccagacgt
     9241 gctggtcaaa ggcggcgatt ggcagccgga gagcatcgta ggcagcaagg aagtgctggg
     9301 ctggggcggc aaggtgcaca gcgtcccgtt tctgcacgag cgctccacta ccgctttatt
     9361 gaaaaagata cgttcgctat gaccatgacg ccaaccgaaa tcacgctgca ccagaaatcg
     9421 aaaacgctgg agatcgcctt cgacgatggc gcacgctaca cgctgccgga cgaatttttg
     9481 cgggtctatt ctccttccgc cgaggtgcga gggcacggcg aggggcaaga ggtgttgcaa
     9541 gtcggcaaga aggacgtcgg catcgtcgag gtggcgccgg caggcagtta tgcgctgaag
     9601 atcaccttcg acgacggtca cgacagcgga ctgtatactt gggaatacct gtacgggctg
     9661 ggacgtgatc aggaaatcct ctggcacaat tatctgcaca ggctggaagc agcaggcgcc
     9721 acgcgcgaag cggccaaata ataattgttc atcggtatag gcggttcatt tccgatggaa
     9781 acttgagatc acaggaataa aaatggcgga tacacatttc ggtttcgaga ccgttgcgga
     9841 agaagaaaaa gccaagcgtg tcgcgggcgt gttcacctcg gttgccagca aatacgacgt
     9901 gatgaacgat ctgatgtcgg tcggcctgca ccgtctgtgg aaacgcttcg cgatcggagt
     9961 gagcggtgtg cgtagcggac agcgcgtgct ggatgtcgcg ggtggctcgg cagacttgtc
    10021 gcgactgttc ttaaagcagg tcggcagcag cggccaggtc gtgctcaccg atatcaacaa
    10081 cgccatgttg cgcgtcggac gcgatcgtct gctcgacgaa ggcatcacca cgccggtgac
    10141 ccagtgcgat gcagaacatc tgcccttccc cgacaattat ttcgactgtg tcagcatcgc
    10201 cttcggcctg cgcaacgtca cgcacaagga tgccgcgctg cgtgaaatga aacgcgtgct
    10261 caagcctggc gggcgggtga tcgtgctgga gttttccaaa gtcgccaagc cactggaaaa
    10321 ggcttacgat ttttactctt tcaagctgct gcccaagatg gggcagctca tcgccaacga
    10381 tgcggacagt taccgctatc tggcggagtc gatccgcatg catcccggtc aggaggagct
    10441 gaagcagatg atggtcgatg ccgggctgga gcgcgtcgag tacttcaatc tgaccggagg
    10501 cgtagttgcg gtgcaccgtg gctacaagtt ctgaacaaca tcatgctgga gatgtcgcca
    10561 tgttccgccg tcacttgccg gactgaattc tagaatcgat atgtcccaga tacctcaaaa
    10621 tgtagaaaga ttggccgtgt tgttcgctga catcagcggc agcaccgcgc tctacgagag
    10681 tcgcggggac gaggttgcgc gccgcctgat cgcccagtgc atcaccgtga tggtgcgcga
    10741 ggttcatgcc tacaagggta cgctgatcaa gaccatcggc gacgagatca tgtgcgtttt
    10801 tccaactgcc gaagaggcct tcctttcttc ttgcgccatg cagaatgccg tagaaaaggg
    10861 gcgcttcgag ggcggttacc ccatgcacat ccgtgtcggc ttccattatg gcgatgtgat
    10921 ccgcgaggca tccgacgtgt tcggcgacac cgtcaacgtt gcggcccggg tcgccgctat
    10981 cacccgcgcc agccagatca tgaccacgca ggctgcggtc gatgcgctgt cgcccgcagc
    11041 ccgcgaaaag acgcgccaga tcttgcgcgc cgaattcaag ggcaagcagg agcaattcga
    11101 catctcgctg gtcatctggg aaatggatga catgatgagc acccgtatcg gaaccccggt
    11161 gttccgcaag acgccggaca atatcgacga attgttgctg cgctatcacg accagacgct
    11221 caaggttaac aaggaacgtc gcagcgtcgt gctggggcgt ggcgagacct gcgatctggt
    11281 cgtgcagaac aacttcgctt cgcgccaaca tctgaagatc gagttgcgtt tcggaaaatt
    11341 cgttgcggtc gatcagagca ccaacggatc ttacgtccgc tttgccgacg gacatgtcat
    11401 gcgcatcgcc cgcgaagaga tcaacctgct cggaaagggc agcatcagca tgggacagcc
    11461 ctactccgat aatgacggag agctgatcga gttttcgatc aactctcccc acgctgcggg
    11521 caaaaacacg gcctgaaaat tcgcttgaat gatcgtcagg cccgggcgtc gcctcgtttg
    11581 tctgcgcccc cttcaaatat caaccaatca aatacaggag acaccatgag cgctttaccc
    11641 aaatgcccga aatgcagttc ggaattcacc tatgaagacg gcagcaatta tgtctgtccc
    11701 gagtgtgcgc atgaatggtc caaggatgcc gccgtagagg gcggcgagca gcagcgcgtg
    11761 tacaaggatg cctttggcaa cgtgctggcc gacggtgatt cggtcacggt catcaaggat
    11821 ctcaagatca agggctcttc ttcggtcgtc aaggtgggca ccaaggtgaa gaacattcgt
    11881 ctggtcgacg gcgaccatga catcgattgc aagatcgacg gcatcggcgc gatgcagttg
    11941 aagaccgaat tcgtgaagaa ggcctgaatc ttcccgcccg agccgcgctt gcctgccgcc
    12001 gatctggaat tgctctatag cgatgcctcg ctgctggtcg tgaacaaacc cgccgggttg
    12061 cttgccgtgc cggggcgcgg agccgacaag caggactgcc tgtcggcgcg gatacaacag
    12121 gaatttgccg atgctttggt ggtgcaccgg ctggacatgg cgacctccgg cttgatggtg
    12181 tttgcacgcg ggactgggat gcagagcgat ttgtcccgtc tgtttcgcga gcgcctggtg
    12241 gagaagcgct acatcgcctg catcgcgggt caaatcagcc cggcaactgg cgagatcgct
    12301 ctgccattgg gggcggactg gcccaatcgc ccgcgccaga aggtcgatgc cgataccggc
    12361 aagccttcgt tgacgcgtta tcgcctgctc gactatgacg acgacagcga tatcagccgc
    12421 atcgaactgg agcccgtcac gggccgcacc caccagttgc gggtgcacat gatggcggcc
    12481 ggccatgcca tcgtcggcga tagattgtat ggcggcaggg aggcatcccg cctgatgttg
    12541 catgcgcgct cgttgagctt cgcccatccg gtcggcggcg agccgctggc tctggcgagc
    12601 gagccgccat tctaggctgt tgccgatgct atacttccgc cctgcttaat taatcacccg
    12661 caaggaaacc tatgaaacgc tttttgatgc tattgaccat tgccatgacc tgtctgagcc
    12721 tgttcgccgc aacggctgag gctaaacgct tcggaggggg cagcagcttc ggtaagcagc
    12781 gctcgatgtc tccccagcag gcccaaaaag cgcccgccgc tgcaccggca cccgctccgg
    12841 gcgcggcacc ggcaaaaccg ggcaacaagt ggctcggccc attggccggc ctggctatcg
    12901 gcgccggatt gggcatgttg ttcgccaaca tgggtatggg tggcgcgatg ggcggcattc
    12961 tgatggcctt gctggcaggc gttgcagtga tgttcctgat ttcgatgttc cgcaagaaac
    13021 agcctcaacc ggccatgcaa tatgccggag ccggagcgcc atacggctca tctcagccgg
    13081 aacagccggg tggcggcagt gctgtcgccg cccccgaggc gcgcagcatc cctgccgatt
    13141 tcccggtgga cagcttcctg cgcagtgcca agaccagctt catccgcctg caggcagcca
    13201 atgaccgcaa ggatctgaac gacgtgcgcg aatacaccac gccggagatg tttgccgaag
    13261 tgtcgatgca gatgcgggag cgcggcgatg ccccgcaaaa gaccgatgtg gttgcgattc
    13321 aggccgacct gctggaagtg gccaacgaag gcaatctggc gatcgccagt gtgcgcttta
    13381 ccggtcagct gtgcgagaac aacggcgcac cggaaagcat cgacgaaatc tggcatgtgc
    13441 aaaaagatct gagtgacgac aagtcggtct ggttgctggc cggcattcag cagactaccc
    13501 tgcactaagc cttcatgttc gcccaacctt cagtcctggt gctgaatcat ctgctgctgc
    13561 agaaccgttg ggcgatgtcc cgtctggccc gctttgcggg aaaaacggcg cgtttcaaca
    13621 tcgcgccgtt ttctttggtg tggactatcc agccggacgg cagtctgcaa ggcgctgacg
    13681 ccgcgatcag cgccgatgcc gtctgcgata ttccgttttc gttgttgccc cgtctggcga
    13741 tgcacgacga gcgggctcat gccgagatcc gtagcgaggg cgatgccgcc ttgctcacgg
    13801 aaatcttctt cctgtcgcgc aacctgcact gggatgcggc cgaagacttg agccacgtca
    13861 ccggtgatgt cgcggccgag cgtatcgtgc aggcggcgca ggatacgaaa cag
//
LOCUS       DASUIV010000194         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1347243, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000194 DASUIV010000000
VERSION     DASUIV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347243"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFW59_07150"
     CDS             <1..231
                     /locus_tag="VFW59_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5364029.1"
                     /translation="ESFFNRAVPEDEPYYLHRDEGADDLPAHLKSSLLGSSLNIPITD
                     GRLRLGTWQGIYLCEHRNHGGSRRIVVTVQGE"
     gene            301..1068
                     /gene="modA"
                     /locus_tag="VFW59_07155"
     CDS             301..1068
                     /gene="modA"
                     /locus_tag="VFW59_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5364030.1"
                     /translation="MMQISLRGFSVTVLVLCGLMSSPVRAGEISVAVASNFAAPMARI
                     AALFQKESGHTVKVSLGASGKLYAQIKGGAPFDAFLSADEELPRLLMQEGSAVGGSRF
                     VYATGRLVLWSAQPDLVDEKGAVLTKGNFNTLAIANPRLSPYGVAAKETLGKLTMWNA
                     IQEKLRKGEDVTQTYQLAAAEKTDLAFIALAQVMRDGKVSEGSWWLVPPEMHHPLRQS
                     AVLLSGAKDQEAAKAFLAYLKSKEARAVMLGFGYELP"
     gene            1274..>2232
                     /locus_tag="VFW59_07160"
     CDS             1274..>2232
                     /locus_tag="VFW59_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5364031.1"
                     /translation="MSPNKNYYWRIAGFYFFYYAFVGMFSPYWSLYLKSIHFNAIEIS
                     ILMSVQPVMRMLAPNLWGWLADHTGKRLLVIQVAAALSALFYLGVFVTTSFWGMLLVL
                     GLMSFFWSASMPLVEATTLSYLGKHTASYGRIRSWGSIGFIVAVVGLGYAFDYIAIAW
                     LLWAGVVCEIGILIFSRQIPHTEVQAHHTDRQPIRQIMLQPRVLALFGACFLMSVAHG
                     PYYTFYSIYLVEHGYAKSAVGGLWALGVICEIGVFFLMPWLVRRYGFTRILIFSFGCA
                     VLRFMLIGYAVDFLTLLLIAQVLHAATFGAYHAASVGMVHESF"
BASE COUNT          393 a          617 c          712 g          510 t
ORIGIN      
        1 gaaagcttct tcaatcgcgc ggtgcccgag gacgagcctt attacctgca tcgcgacgaa
       61 ggcgcggacg atctgcccgc gcatctcaaa tccagcctgc tcggcagcag cctgaacatc
      121 ccgatcaccg atggccgcct aaggctggga acctggcagg gcatctatct gtgcgaacat
      181 cgcaaccacg gcggcagccg ccgcatcgtc gtgacggtgc agggcgagta ggcatccgcc
      241 agaccgttac gcggacttca tcttgcttgt tatgatggca accattccag tcaggagttg
      301 atgatgcaaa tttcattgcg cggctttagc gtcacggttc tggtgttgtg cgggctgatg
      361 agttcgccgg tgcgcgcggg cgagatcagc gttgcggtgg catcgaattt cgccgcgccg
      421 atggcacgca tcgcggcgtt gttccagaag gaaagcgggc acacggtgaa ggtcagtctg
      481 ggggccagcg gcaagttgta tgcgcagatc aagggcggcg cgccgttcga tgcatttctt
      541 tccgcagacg aagagctgcc gaggctgttg atgcaggagg gctcggcggt cggtggctct
      601 cgcttcgttt atgcgacagg caggctggtg ttgtggagtg cgcagcccga tcttgtggac
      661 gaaaaaggcg cagtgctcac caagggcaac ttcaatacat tggctatcgc gaatccccgg
      721 ttatcgccat acggcgtcgc ggcgaaagag accttgggga aattgacgat gtggaatgcg
      781 atacaggaaa agctgcgcaa gggcgaggat gtcacgcaga cttaccaatt ggctgcggcg
      841 gagaagaccg atctggcttt catcgcgctg gcgcaggtca tgcgtgacgg caaggtatcc
      901 gagggttcct ggtggctggt gccgcccgag atgcatcatc ccctccggca aagcgcggtg
      961 ctgttatccg gcgcgaaaga tcaggaagct gccaaggcct ttcttgccta tctcaagagc
     1021 aaggaagcgc gggccgtgat gctcggtttc ggttacgagt tgccttgagg taaggatgcg
     1081 ctgaacaagt cttccgttcg tggtgagctt gtcgaaccat gaacggagga caccaactaa
     1141 tcagcggcca gcaaagttcc cccttcgaca ggctcagggc gaacggattt gtttagtgca
     1201 tccttaacgc gagttccggc gcggcaattt tctccgcatg tgataatgcg cgccattcag
     1261 atgccgcatt gccatgtctc ccaataaaaa ttattactgg cgcatcgccg ggttctattt
     1321 tttctactat gccttcgtgg ggatgttctc gccctactgg agcctgtatc tcaagtccat
     1381 tcatttcaac gccatcgaga tttccatcct gatgtcggtg cagccggtga tgcgcatgct
     1441 tgcgcctaac ctgtggggct ggctggccga ccataccggc aagcggttgc tggtgataca
     1501 ggtggcggcg gcgttgagcg cgctgttcta tctgggcgtg ttcgtgacga ccagtttctg
     1561 gggcatgctg ctggtgctgg ggctgatgag ttttttctgg agcgcgtcga tgccgctggt
     1621 cgaggcgacc acgctttctt atctaggcaa gcataccgcg agctacgggc gcatccgctc
     1681 gtgggggtct atcgggttca tcgttgcggt ggtcgggctg ggctacgctt tcgattacat
     1741 cgccatcgca tggctgttgt gggcgggcgt ggtgtgcgag atcggcatcc tgattttttc
     1801 gcggcagatt ccgcacaccg aggtgcaggc gcatcacacc gaccgtcagc cgatccggca
     1861 aatcatgttg cagccgcgcg tgctggcgtt gttcggcgca tgcttcctga tgtccgtcgc
     1921 gcacggcccg tactacacgt tctattccat ctatctggtc gagcacggct atgccaagag
     1981 cgcggtgggc gggctgtggg cgctcggggt gatctgcgag atcggcgtgt tttttctgat
     2041 gccctggctg gtgcgccgct acggcttcac ccgcatcctg atcttcagct tcggctgcgc
     2101 ggtgctgcgc ttcatgctga taggctacgc ggtggacttc ctgacgctgc tgctgatcgc
     2161 gcaagtgctg cacgccgcca ccttcggcgc atatcacgcc gcatcggtgg gcatggtgca
     2221 cgagtccttc ca
//
LOCUS       DASUIV010000195         9344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_974867, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000195 DASUIV010000000
VERSION     DASUIV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974867"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /gene="thyA"
                     /locus_tag="VFW59_07165"
     CDS             <1..331
                     /gene="thyA"
                     /locus_tag="VFW59_07165"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX5364032.1"
                     /translation="EGRLSCQLYQRSADVFLGVPFNIASYALLTMMMAQVCGLKYGDF
                     VHTFGDAHLYSNHMEQTREQLSREPRPLPTMKINPEVKDILGFKFEDFTLEGYDPHPA
                     IKAAVAV"
     gene            328..813
                     /locus_tag="VFW59_07170"
     CDS             328..813
                     /locus_tag="VFW59_07170"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX5364033.1"
                     /translation="MSLSLIVAMAKNRTIGVNNTLPWRCPEDLKHFKALTMGHHMIMG
                     RKTFDSIGKPLPGRTTVVVTRDRNLAIEGCLIAHSIPEAIKACANDAEIFIVGGADIY
                     TQTLPLADTLYITEIQQDVEGDAHFPEFARNDWQEEAREVHTQETPQPLEYHFVSYRR
                     I"
     gene            870..1421
                     /locus_tag="VFW59_07175"
     CDS             870..1421
                     /locus_tag="VFW59_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEX5364034.1"
                     /translation="MAIKATVFKANLQIADMERHYYQDHALTLARHPSETDERMMVRV
                     LAFALHAHEYLEFGQGMTNDDEADLWRKDLTGAIELWIDVGIPDEKLIRKACGRADQV
                     VVYCYGGRVADMWFAQNSAQFARQKNLTIVNLPPASTQALAKLAQRTMNLQCTIQDGQ
                     VWLGDGESSVQVERAILKAPETR"
     gene            complement(1480..2745)
                     /gene="nhaD"
                     /locus_tag="VFW59_07180"
     CDS             complement(1480..2745)
                     /gene="nhaD"
                     /locus_tag="VFW59_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HEX5364035.1"
                     /translation="MDIWLVTIFVIAYAAIACEHPLKINKSVPALIGAGLLWTIYAAN
                     TGHVSEQLNESLSATAQIVFFLMGAMAIVEVVDAHDGFDVITDKIKTTSMVKLLWLVG
                     FVTFFLSAVLDNLTTTIVMISVCRKLLDQREDRLFFAGVTIIAANAGGAWSPIGDVTT
                     TMLWIGGQITALAIIKGLFIPSLVNMMVPLAVISYMYRGRAVVGPVKVANISVRYETT
                     PFESSFMFYAGIGLLCAVPVFKTLTHLPPFMGILFALGILWLVGELIHRDKEDQQKEP
                     LTIGAALQRIDMQSLVFFIGILLAVATLEHSHILAALAEWLNTTIGSQAIIVFVFGLV
                     SAIVDNVPLVAASMGMYSLTEFPPDSFLWEFLAYAAGTGGSILIIGSAAGVAAMGIEK
                     IEFFWYVRKVSLLALIGYIGGGLAYIAIH"
     gene            complement(2978..4213)
                     /locus_tag="VFW59_07185"
     CDS             complement(2978..4213)
                     /locus_tag="VFW59_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5364036.1"
                     /translation="MSTTILVIGLMVFFAHFLSLQFRKTNIPDVLVLMLVGILIGPLL
                     GVVTSEDFGKIGSLIATIALVVILFESGTSLNLGILGQSLATTGWLSFACFVLTFCVV
                     ALVGMVVLDLGLLPAILLGVTVGGTSSAVVIPMVGALRMGEKAATVLVLESALTDVLC
                     IVGVFALLQIYTQGGVEPGRLVGGVLAALIFAAVIGLIGGIGWLLVLGKVRDFPNTMS
                     STLAYVFIVYGSTEILGFSGAIAALALGITLTNFEKFGLTRFDRIDRNIVPLNEIDLA
                     FYREAVFLLKTYFFVYLGISIHFGAAKLAVAAGIIVLLVYAMRLFLTRLVFRAEAYSL
                     RDAAITSMMAPKGLAAAVLAALPLQYGVAGGEVIRDVTYMVVLVSITFTAVLVMLYPL
                     PAAQKLYSRVLAKPLADIG"
     gene            4429..6399
                     /locus_tag="VFW59_07190"
     CDS             4429..6399
                     /locus_tag="VFW59_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX5364037.1"
                     /translation="MSDHSQLLELLAPAKTAAIGRDAILHGADAVYIGGPSFGARDKA
                     GNSIADIAGLVAFAHRYRAKIFVTLNTILHDAELEAARQQAYACYEAGVDALIVQDMG
                     LLELDLPPIDLHASTQCDIRTPEKARFLAEVGFSQIVLARELTIKEIAKVRAAVPADT
                     VIEHFIHGALCVAYSGQCNISHAHTGRSANRGDCSQACRLPYTLTDAQGQVVAFEKHL
                     LSVKDNNQSDNLRALIDAGVRSFKIEGRYKEAPYVKNITGHYRLLLDEILEERPELSA
                     ASSGKTKLLFTPNPDKTFHRGATDYFSNGRKADIGAFDTPSFVGLPLGSLTKIGADHF
                     ELESNEALANGDGLNYLNKREVTGIRANKVQRIGESANGELWKVWPNEAIAALPGLHP
                     GLTINRSRDHVWEQLLSKKSAERRIGVSVRFAETGNGFSLTLQDEDGICATSSLAADK
                     QPAQHPEQAEAALREQLDRFGSSDFSSHSIAVDWSQPWFVPSSALNQLRRDAVERLEA
                     ARAQAYVRLQRKAAVEPPAQYPEETLSFLANVYNQSARAFYARHGVKLIEAAYEAHGE
                     TGEVSLMVTRHCLRYSMSMCPKQAKGVTGVQGKVRAEPMTLVNGSERLTLKFDCKACE
                     MHVMGKIKKHILKTPPPSVVPVTFHKRRPDAA"
     gene            6545..8167
                     /locus_tag="VFW59_07195"
     CDS             6545..8167
                     /locus_tag="VFW59_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HEX5364038.1"
                     /translation="MQQDANLKFLSIHLDEKGRKVVASFTPEASVEPATPEAFTQAVE
                     AAGFGGYSLYQVPIAQAIAKYNTGSAFEIAIGEALDGKLSVRIDPNMMAAYLSCTLPQ
                     GGAPVQRQDVLDEAAKRGITAALDLEAIDRTLRDGGDNVQIATGRSPVPAVDGKFEIL
                     FPSMKVRSPQLDEHGLANFRELGGIVTVEAGDKLMRIVAPVEGEPGETVTGKIIPVKP
                     GKKVSFSSKLVGVAIDANDPNLLIATIPGSPVALKDTATVEPICIIKDVDLHTGNITF
                     NGTIHVSHDVHVGMTLIATGDIYVDGTVENALLEAGGDIIVKGGIIGASELHANPNEK
                     FHSAIRCDGSCTARFVQNVHITAGNGIFIHDLAMLSELTAGHQIVVGDENSRKGEIIG
                     GSARATMLVKAKNIGSDSSVRTVVVVGADQRLHERLNNTIKDREAAEHKLADIIKLLE
                     LSLSHPGRIPPETIRTAEATRDALTVEIETLREVEAGLNQEISLADGAQVIVEKHVFA
                     GAEIRIGLEHYTASELKEGGVFHISEDKLVFD"
     gene            complement(8300..8620)
                     /locus_tag="VFW59_07200"
     CDS             complement(8300..8620)
                     /locus_tag="VFW59_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5364039.1"
                     /translation="MKIVHLMAMAAALSVSASVFAADDGEALFKKSGCVACHTVEKKS
                     VGPSMKDVAAKYKDDKEAQAKLQEKVRNGGKGSFGTMPMPATKASVSDENIKSIVEWA
                     LSRK"
     gene            8964..>9344
                     /locus_tag="VFW59_07205"
     CDS             8964..>9344
                     /locus_tag="VFW59_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5364040.1"
                     /translation="MENSNRELLTFTLGSEEYGIDILKVQEIRGYDAVTTIANSPEFI
                     KGVINLRGIIVPIVDMRIKFNLGNVTYNELTVVIILNIANRVVGMVVDGVSDVIALTA
                     DQLKAAPEFSGTMDTQYITGLGTID"
BASE COUNT         2298 a         2873 c         2493 g         1680 t
ORIGIN      
        1 ggagggccgg ctttcctgcc aactctacca gcgctccgct gacgtgtttc tcggcgtgcc
       61 gttcaacatc gccagctacg cgctgctgac gatgatgatg gcgcaagtct gcggcctgaa
      121 atacggcgac ttcgtgcaca ccttcggcga cgcgcatctg tattcgaacc acatggagca
      181 gacccgcgaa cagttgtcgc gcgagccgcg tccgctgccg accatgaaga tcaacccgga
      241 ggtgaaagac atcttgggct tcaagttcga agacttcaca ctggaaggtt acgacccgca
      301 tccggcgatc aaggctgcgg tggcggtatg agcctctccc tcatcgttgc gatggcgaag
      361 aaccgcacca tcggcgtgaa caacacgctg ccctggcgct gcccggaaga cctgaaacat
      421 ttcaaggcgc tgaccatggg gcatcacatg atcatggggc gcaagacctt cgactccatc
      481 ggcaagccgc tgccgggacg caccacggtg gtggtcacgc gcgaccggaa tctggccatc
      541 gaaggctgtt tgatcgctca ctcgatacca gaggcgatca aggcatgcgc aaatgatgca
      601 gagattttca tcgtcggcgg cgcggacatc tacacacaga ccctgccgct ggccgacacg
      661 ctgtacatca ccgaaataca gcaagacgtg gaaggcgacg cccacttccc ggaattcgcg
      721 cgcaacgact ggcaggaaga ggcgcgcgag gtgcatacac aggagacgcc gcagccgctg
      781 gaatatcact tcgtcagcta tcgccgcatc tgatcgggca cgacccgcac aactcaccgg
      841 cacgacatgc tgtgccgctc aaggaaatca tggcaatcaa ggcaaccgta ttcaaggcca
      901 atctgcaaat cgcggatatg gagcgtcact actatcagga tcacgcgctg acgctggctc
      961 ggcatccgtc tgaaaccgac gagcgcatga tggttcgcgt gctggcgttt gccttgcacg
     1021 cccacgaata tctggaattc ggtcagggca tgaccaacga cgacgaggcc gacctgtggc
     1081 gcaaggatct gaccggcgcc atcgagctat ggatcgacgt cggcatcccc gacgagaagc
     1141 tgatccgcaa ggcgtgcgga cgcgccgatc aggtggtggt gtattgctac ggcggacgcg
     1201 tcgccgacat gtggttcgcg cagaacagcg cccagttcgc gcgccagaag aatctcacca
     1261 tcgtcaatct gccaccggcc agcacgcagg ccttggccaa gctggcacag cgcaccatga
     1321 acctgcaatg caccattcag gatgggcagg tgtggctggg cgatggcgaa tccagcgtgc
     1381 aggtggaacg ggccatactg aaagcgccag aaaccagatg aagtacatgc ttaaaagttt
     1441 tcctttgcat gtacgccatc aagtgagttt tcgcccgcct caatggatcg cgatatatgc
     1501 aagtccgcca cctatatagc cgataagcgc aagcaagctg acctttctga cataccagaa
     1561 gaattcaatc ttctcgatac ccatcgccgc cacacccgct gcagaaccaa tgatcagaat
     1621 cgatccgccc gttcctgccg cgtaagccag aaactcccag agaaacgaat ctggcgggaa
     1681 ctcagtcaat gaatacatgc ccatggaggc ggccaccagc gggacgttgt ccacgattgc
     1741 gctgaccagt ccaaaaacaa agacaatgat cgcctgactg ccgattgtgg tatttagcca
     1801 ttcggcaagc gcagcaagga tatggctgtg ttcgagcgtt gccaccgcaa gcaaaatacc
     1861 aatgaagaac accagcgatt gcatatcgat tcgctgcagg gcagcaccaa tcgtcagggg
     1921 ctctttctgt tgatcttctt tgtcgcgatg aatcaattca ccaactaacc agaggattcc
     1981 aagggcaaac aaaatcccca taaacggggg caagtgggtc aaggtcttga aaacagggac
     2041 ggcgcacaac aggccgatcc cggcatagaa cataaagctg ctctcaaatg gagtcgtctc
     2101 gtatcgcacg ctgatattcg ccaccttgac ggggccgaca actgcccttc ctcgatacat
     2161 atagctaatc actgccaacg gaaccatcat gttcaccaag gaagggataa acagcccctt
     2221 gatgattgcg agagcggtaa tctgcccgcc tatccagagc attgtagtcg tgacgtcccc
     2281 tatcggcgac caggctccac cggcattggc ggcaataatg gtcacccccg caaaaaacag
     2341 ccggtcttct ctttgatcca acaattttcg acacacagat atcatgacga tagtcgtcgt
     2401 cagattatcc agaacagcac ttagaaagaa cgtaacgaaa ccgaccagcc acagcagctt
     2461 aaccatactg gttgtcttga tcttgtccgt aatcacatcg aagccatcat gggcatccac
     2521 aacctcaacg attgccattg ctcccatcag gaagaaaaca atctgcgccg ttgcagaaag
     2581 actttcattt agctgctcgg agacatgtcc tgtgttagcc gcgtagatgg tccacaacag
     2641 tccagcccca attagggctg gaacgctctt gttgatcttg agagggtgct cacaagcgat
     2701 tgccgcataa gcgatgacaa aaatcgtgac taaccatata tccattatca tttctccgct
     2761 gaggcgctgt tttcaatttt ctcgttcagt ttgcgcatcc aggaaccgca actaaagcat
     2821 cagagaaacc cgttcttatt ttttctgccg ggtatagctg aaacaaggaa agtgccactc
     2881 ttgccatttc cctgtatgaa taggattgat gctgctcgtc accatccaaa ctgctaccgg
     2941 ctttgcctaa taacgaacaa ggatttcccc ttctaactta accaatatct gcaagcggct
     3001 tagccagcac acggctataa agtttctggg ctgcgggcaa ggggtaaagc attaccagca
     3061 cagcagtgaa cgtaatgctg accagcacca ccatataagt gacatcgcgg atcacttcgc
     3121 cgcctgccac gccgtactgt aacggcagtg cggccagcac ggcggcggcc agtcctttcg
     3181 gcgccatcat cgaggtgatc gcggcatcgc gcagactgta cgcttccgcg cgaaatacga
     3241 gccgggtcaa gaacaatcgc atcgcataaa ccagcagcac gatgatgcct gccgcaacgg
     3301 cgagcttcgc tgcgccaaag tgaatagaga tgccgagata gacaaagaaa taggttttga
     3361 gcaggaacac tgcctcgcga tagaaggcca agtcgatctc attcaacggc acgatatttc
     3421 ggtcgatacg atcaaagcgg gtcaggccaa atttctcgaa attggtcagc gtgataccca
     3481 gtgccagcgc ggcaatcgca ccggagaagc cgagtatctc ggtcgaacca tagacaataa
     3541 aaacgtaagc cagcgtggaa gacatcgtgt tcgggaaatc gcgcaccttg cccagcacca
     3601 acagccaacc aatacctcca ataagcccga taaccgcagc gaagatcagc gcggcgagta
     3661 cgccgccgac caacctgccg ggctcaacac ctccctgagt gtaaatctgc agcagcgcaa
     3721 acacgccgac gatgcacaac acgtcggtca gtgccgattc cagcaccaga acggtggctg
     3781 ctttctcgcc catacggagc gcaccgacca tgggaataac cacggcagaa gaggtgccgc
     3841 ccaccgtaac accgagcaaa attgcgggca acagaccaag atccagcacc accatcccga
     3901 caagcgccac gacacaaaaa gtcagcacaa aacaggcgaa gctcaaccag ccggtagtcg
     3961 ccaacgattg gcccagtatg cccagattca gcgaggtgcc gctctcgaac agaatcacca
     4021 ccagcgcgat ggtggcgatc agcgaaccga tcttgccgaa gtcttcagat gtgacgacgc
     4081 ccagcaacgg cccgatcaga ataccgacca gcatcagcac cagcacgtcg gggatattgg
     4141 tcttgcggaa ttgcagcgac agaaagtgtg cgaagaacac catcagtccg atgacaagga
     4201 ttgttgtgga catcaggatg tctcctattg aacgaatttg attatacata tcatccggtt
     4261 tctggatgtc tgccaccggc gaaccgacat tcaggcagat cagagcatct gatccgggag
     4321 cggctcctat ccgcatcaat ggcactcgaa taccaattac gctccaatgc cgacatccat
     4381 tccatccggc acaaggcaac tcaccgcctc cggtataatc cgccgcctat gtcagaccat
     4441 tcccaactcc tcgaactgct cgctccggcc aagacggccg ccatcggacg cgatgccatc
     4501 ctgcacggcg ccgacgcggt ctatatcggc gggccgtcgt tcggcgcgcg cgacaaggcg
     4561 ggcaactcga tcgccgacat cgccggactg gtggcattcg cacaccgcta ccgcgccaag
     4621 atattcgtca cgctgaacac catcctgcac gacgccgaac tggaagcggc gcgccagcag
     4681 gcatacgctt gctacgaagc gggcgtggac gcgctgatcg tgcaggacat gggactgctg
     4741 gaactcgatc tgccgccgat cgacctgcat gcctccaccc agtgcgacat ccgcacgccg
     4801 gaaaaggccc gctttctggc agaggtcggc ttctcgcaga tcgtgctggc gcgcgaactg
     4861 accatcaagg agatcgccaa agttcgcgcc gccgtgcccg ccgacaccgt gatcgaacac
     4921 tttatccacg gcgcgctgtg cgtggcctat tccggccagt gcaacatcag ccatgcgcac
     4981 accggacgca gtgccaatcg cggcgactgt tcgcaggcct gccgcctgcc ctacacgctg
     5041 accgatgctc aaggacaagt cgtcgcgttc gagaagcatc tgttgtcggt gaaggacaac
     5101 aaccagagcg acaacctgcg cgcgctcatc gatgcgggcg tgcgcagctt caagatcgag
     5161 gggcgctaca aggaagctcc ctacgtgaag aacatcaccg gccactatcg tttgctgctg
     5221 gacgagatcc tcgaagagcg gcccgaactg agcgcggcat ccagcggcaa aacaaaattg
     5281 ctgttcaccc ccaacccgga caagaccttc catcgcggcg cgacggatta tttctccaac
     5341 ggccgcaagg cggatatcgg cgcgttcgac acgccctcct tcgtcggcct gccgctgggc
     5401 tcgctgacga agatcggcgc agaccatttc gagctggaat cgaacgaagc gctggccaac
     5461 ggcgacgggc tcaattacct gaacaaacgg gaagtgaccg gcatacgcgc gaacaaggtg
     5521 caacgcatag gcgaaagcgc gaacggcgag ctgtggaagg tctggccgaa cgaggccatc
     5581 gccgccctgc ccggcctgca tccggggctc accatcaacc gcagccgcga ccatgtctgg
     5641 gagcagctgc tcagcaagaa atccgccgaa cgccgcatcg gagtgagcgt gcgctttgcc
     5701 gagaccggca atggcttttc gctcacactg caagacgaag acggcatctg cgcgacctcg
     5761 tccctcgctg ccgacaagca gcccgcacaa cacccggaac aggccgaagc cgcgctgcgc
     5821 gagcaactcg accgcttcgg cagcagcgat ttttcctcgc actcgatcgc ggtcgactgg
     5881 tcgcaaccct ggttcgtacc cagctccgca ctgaaccaac tgcgccgcga tgcggtggaa
     5941 cgactcgaag ccgcgcgcgc ccaggcttac gttcggctgc aacgcaaagc cgccgtcgag
     6001 ccgcccgcgc agtatcctga ggagacgctg tccttcctcg ccaatgtcta caatcaatcg
     6061 gcgcgcgcct tctatgccag acacggcgtg aagctgatcg aggcggccta cgaggcgcac
     6121 ggcgagaccg gcgaggtatc gctgatggtc acccgccact gcctgcgcta ctcgatgagc
     6181 atgtgcccga aacaggccaa aggcgtgacc ggggtgcaag gcaaggtgcg cgccgaaccg
     6241 atgacgctgg tcaacggcag cgagcgcctc accctgaagt tcgattgcaa ggcctgcgag
     6301 atgcatgtca tgggcaagat caagaaacac atcctgaaga ctccgccccc ctcggtggtg
     6361 ccggtaacat tccacaagcg ccgccccgac gccgcgtgaa taaccaggcc gcccctgttt
     6421 ttttccgcat gacgatcccg aaccatcagc ggacgatctc gtaaggtatt acgcactact
     6481 tccccacagg gatagttggc tatactgacg gagcggcatg tgtctgagcc atcaggggaa
     6541 aacaatgcaa caagatgcga atcttaaatt cctgtccatt catctggatg aaaaaggaag
     6601 aaaggtagtg gcgagcttca cgccggaagc aagcgtggaa cccgccacac ccgaggcgtt
     6661 cacgcaggcc gtcgaggccg ccgggtttgg cggatacagc ctgtaccagg tgcccatcgc
     6721 gcaggccatc gctaaataca ataccggttc agcgtttgag atcgcgattg gcgaggcgct
     6781 ggacggcaag ctctccgtac gcatcgatcc caacatgatg gcggcttatt taagctgcac
     6841 cctgccgcag ggcggtgcgc cggtgcaacg acaggatgtg ctggacgagg ccgccaaaag
     6901 aggcatcact gccgcgctgg atctcgaagc catcgaccgg acgctgcgcg atggcggcga
     6961 caatgtacag atcgccacgg gaagatcgcc cgtgcccgcc gtagacggca agttcgaaat
     7021 cctgtttccc agcatgaaag tccgcagccc ccagctcgac gaacatgggc tggccaattt
     7081 ccgcgaactg ggcggcatcg tcaccgtcga ggcgggagac aaactgatgc gcatcgttgc
     7141 accggtcgaa ggagaaccgg gagagaccgt gaccggcaag atcattccgg tcaaacccgg
     7201 aaaaaaagtt tcattttcat ccaagctcgt gggcgtcgcg atcgatgcca acgatcccaa
     7261 cctgctgatc gccacaatcc ccggcagccc ggtggccttg aaagacacgg ccaccgtaga
     7321 gccgatctgc atcatcaagg atgtcgacct gcataccggc aacatcacct tcaacggtac
     7381 gatacatgtg tcccatgacg ttcatgtcgg catgacgctc atcgccaccg gggatatcta
     7441 tgtcgatggc acggtcgaaa acgcgctgct ggaagcgggc ggcgacatca tcgtcaaggg
     7501 gggcatcatc ggcgcctcgg aactgcacgc caatccgaac gaaaaattcc actccgcgat
     7561 ccggtgcgat ggctcgtgta ccgcccgctt cgtgcagaac gtccacatca ctgcgggaaa
     7621 cggcatcttc atccacgacc tcgccatgct cagcgagctg actgccgggc accagatcgt
     7681 tgtcggcgat gaaaacagcc gcaaaggcga aatcatcggc ggctccgccc gcgccaccat
     7741 gctggtcaag gccaagaaca tcggctccga ttccagcgtg cggacggtgg ttgtggtcgg
     7801 cgcggatcag cgcctgcatg aacggctgaa caatacgatc aaggatcgcg aagcggccga
     7861 acacaagctt gccgacatca tcaagctgct cgaattgagc ctgtctcacc ccggacgcat
     7921 accacctgaa accatcagga ctgcagaggc gacacgcgac gccctcactg tcgagattga
     7981 aactctgcgg gaagtggaag caggattgaa tcaggagatc agtctcgccg acggcgcgca
     8041 ggtgatcgtg gaaaaacatg tcttcgccgg cgccgaaata cgcatcggcc tggaacatta
     8101 caccgcgtca gaactcaagg aaggcggcgt gttccatatc agcgaagaca aacttgtttt
     8161 tgactgaccg gaggaatgcc atcgcgcgca gctggctcaa tctcccgcgc agccgagcag
     8221 cgcgtccatt gagccactca ggaaaatttc cgcgcataaa aaaagccgac tcgcgtcggc
     8281 tttttattca gaccagaact tacttgcggc tcagcgccca ttccacaatg ctcttgatat
     8341 tctcatcgct aaccgaagcc ttggttgccg gcatcggcat cgtaccgaaa gaacccttgc
     8401 cgccattgcg cactttttct tgcagcttgg cttgagcctc tttgtcgtcc ttgtacttcg
     8461 ccgccacatc cttcatcgac ggaccgacag acttcttctc taccgtatga catgctacgc
     8521 agccactttt cttgaacagt gcctcaccgt catccgcagc aaatacgctg gcagaaacag
     8581 acagtgctgc ggccatggcc atcaaatgaa caatcttcat aacattctcc ttttgtgaaa
     8641 aaacggtaag aacattctac acccgaagcg ccgcaggaat acaggctgaa cagaatcttc
     8701 ctcacgcaag ccaaaaacat tttcagacag gcatcacccc catggaacat ggccggcgcg
     8761 caacataacg gaacgcccga gaacgtttaa accatggcag atacacacca gaaagattag
     8821 cttcgctcac cccctgcgcg aatgacccga cgccccggat aaagttatcg ttcatcggct
     8881 ggcacaatcc ctgcccccgc tttacaatgc aaattgcata aacactccag acaacgacca
     8941 tgcaagaaga tcaggctcta aagatggaaa acagcaaccg tgaattactg acattcacac
     9001 tcggcagcga agaatacggt atcgacatcc tcaaggtgca ggagatacgc ggctacgatg
     9061 cggtgaccac catcgccaac tcgccggagt tcatcaaggg cgtgatcaat ctgcgcggca
     9121 tcatcgtgcc gatcgtcgac atgcgcatca agttcaacct cggcaacgtg acctataacg
     9181 aactgacggt ggtcatcatc ctgaacatcg ccaatcgcgt ggtgggcatg gtggtggacg
     9241 gcgtgtccga cgtgatcgcg ctgacggccg accagctcaa ggccgcaccg gaattcagcg
     9301 gcacgatgga tacccagtac atcaccggac tgggtacgat tgac
//
LOCUS       DASUIV010000196         7165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_882044, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000196 DASUIV010000000
VERSION     DASUIV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882044"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFW59_07210"
     CDS             complement(<1..1322)
                     /locus_tag="VFW59_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5364041.1"
                     /translation="MIAQELEVSLHLAFVEARQKRHELITVEHLLLAMLDNPSAAQIL
                     RSCNVDLDDLRRVVSAFINTHTPIVPGDGEVDTQPTVGFQRVIQRAILHVQSANKKEV
                     VGANILAALFGEKDSHAVHFLTERGVNRIDVVNQIVHGITKGDSGKAAQPQVEGEAEQ
                     PAADEGVLKNYTLDLNAHALAGKIDPLIGRDLELERVIQTLCRRRKNNPLLVGEAGVG
                     KTAIAEGLARRIVENEVPEILRDAQVFSLDMGSLLAGTKYRGDFEQRLKSVLKELSAI
                     PNAVLFIDEIHTMIGAGAASGGTMDASNLLKPALSNGTLKCIGATTYQEYRGIFEKDH
                     ALSRRFQKVDVPEPSVEQTIEILKGLKSRFEEHHSVKFSAAAITSAAELSARFINDRH
                     LPDKAIDVLDEAGAAQRILPKSKQKKTIGKHEIEEIIAKIARIPTRTVS"
     gene            complement(1324..1632)
                     /gene="clpS"
                     /locus_tag="VFW59_07215"
     CDS             complement(1324..1632)
                     /gene="clpS"
                     /locus_tag="VFW59_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX5364042.1"
                     /translation="MANQQQSNSVLAPEQTKIKPPPMYKVVLFNDDYTTMDFVVDVLQ
                     RFFAMNRECALQTMLKIHNDGSASCGVYSRDVAETKVVQVTEYAKQHGHPLRCGTEEI
                     "
     gene            1875..2078
                     /locus_tag="VFW59_07220"
     CDS             1875..2078
                     /locus_tag="VFW59_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5364043.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGSEDLFAHFSAINMSGFKSLKE
                     GQKVSFDLVQGPKGKQASNIQAA"
     gene            complement(2148..2852)
                     /locus_tag="VFW59_07225"
     CDS             complement(2148..2852)
                     /locus_tag="VFW59_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364044.1"
                     /translation="MKKSRIALLILLAIATVALYLWFNNPLNGLVKSAIEKFGSEMTQ
                     TQVSVSRVKLSASNGRGTISDLSLGSPKGFKSPHAFKVDHIEVAIEAATVTDDVILIH
                     KILIAAPDIVYEKNDSGTNFHAIQRNVERYLGSDKKPSPADKKTSKKMIIESLVIRDI
                     KVSYNGMLNLTLPDIELHNIGKRNGGATSAQVVNAIIGELNTKMAISLAKTAAIGAVG
                     GVAVGVGMGIKSLLGK"
     gene            complement(2973..5195)
                     /locus_tag="VFW59_07230"
     CDS             complement(2973..5195)
                     /locus_tag="VFW59_07230"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX5364045.1"
                     /translation="MTSPKIIYTMVDEAPALATYSLLPIVQAFTKAAGVTVETRNISL
                     AGRILANFPEHLTAAQKIADELTELGELTLKPEANIIKLPNVSASSPQLKAAIKELQS
                     QGFNIPDYPDDPKTDAEKDIKARYGKVLGSAVNPVLREGNSDRRAAASVKQFARKNPH
                     KMGAWSSDSRTHVAHMNTGDFYGSEKSVTVSEAGSFKIEFVGQDDNVTVLKDKTPVKA
                     GEIIDACVLSRRALRDFYEAQMQDAKQQGVLLSLHLKATMMKISDPIMFGHAVTVYFK
                     DVFEKHAATIKKLGVNVNNGFGDLIAKIASLPEDQRAEIEADIQACYQSRPELAMVNS
                     DKGITNLHVPSDIIVDASMPAMIRDSGKMWGTDGKLHDTKAMIPDRCYARIYQVVIDD
                     CKKHGAFDPKTMGSVPNVGLMAQQAEEYGSHNKTFEAPANGKIRAVDESGKTLLEQDV
                     EQGDIFRMCQAKDAPIQDWVKLAVTRARLSNTPAVFWLDKNRAHDAQIIRKVERYLKD
                     HDTSGLDISIMTPEEAIQFSLDRIRAGLDTISVTGNVLRDYLTDLFPILELGTSAKML
                     SIVPLMNGGGLFETGAGGSAPKHVQQFQQEGYLRWDSLGEFLALGVSLEHLAQTFKNA
                     KAQVLADTLDQANGKILENNKSPARKIGELDNRGSHFYLALYWAQALAAQDKDAALKA
                     KFAPLAKVLTDNEAKINAELIAAQGKPVDMGGYYHPDFDKTSKAMRPSATFNAALDAI
                     "
     gene            5442..5984
                     /locus_tag="VFW59_07235"
     CDS             5442..5984
                     /locus_tag="VFW59_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEX5364046.1"
                     /translation="MSRILLFNKPYGVICQFSRDGLHPTLADYIVLPDFYPAGRLDTD
                     SEGLLLLTDDGKLQHRITDPKHKLPKTYWVQVEGVPDEVALEQLRRGVQLKDGMTLPA
                     GARLIDEPAMLWPRNPPIRERKAIPASWLELTIREGRNRQVRRMTAAVNFPTLRLIRY
                     AIGDWTLDGIAPGQWRQEEV"
     gene            6015..6326
                     /locus_tag="VFW59_07240"
     CDS             6015..6326
                     /locus_tag="VFW59_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364047.1"
                     /translation="MLRNSILFVALLSLGMPASAGTPREIAGKPAAKLVGTWHSAQET
                     ITFKPNGLVVYKGHSHYFAAGNGVIQIKSKHVIRNLPYRMFDGKLTVTDNGVDTVYTR
                     E"
     gene            complement(6475..>7165)
                     /locus_tag="VFW59_07245"
     CDS             complement(6475..>7165)
                     /locus_tag="VFW59_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5364048.1"
                     /translation="AAAIHGNKSQSARTKALAQFKDGSLPVLVATDIAARGLDIDMLP
                     HVVNFELPNVPEDYVHRIGRTGRAGSNGAAISLVDSEEFQHLKNIERLIKHQIPKVAI
                     DSFVPPTNIPADAPRAPRPQHGQKRHSNTTASAPRTAQKPRDGQAPRQPREQQARNGQ
                     QPRTAQPGNGGQPGKPRQPQPKKTGDQALSEAARHQAMPQPGLFQPAPRVRREHVAGK
                     PAGVPRGRGRG"
BASE COUNT         1256 a         1999 c         2195 g         1715 t
ORIGIN      
        1 gacacggtgc gggtcgggat gcgcgcgatc ttggcgatga tctcctcgat ctcgtgcttg
       61 ccgatggtct tcttctgttt cgatttcggc aggatgcgct gtgcggctcc ggcttcgtcg
      121 agcacgtcga tggccttgtc gggcagatgg cggtcgttga tgaagcgtgc ggaaagttcg
      181 gccgcgctgg tgatcgccgc cgcgctgaac ttgacgctgt ggtgttcctc gaagcggctc
      241 ttcagcccct tgaggatttc gatggtctgc tcgaccgaag gctcgggtac gtccaccttc
      301 tggaagcggc gcgacagcgc gtgatccttc tcgaagatgc cgcgatattc ctgataggtg
      361 gtcgcaccga tgcatttaag cgtgccgttc gacagcgcgg gcttgagcag gttcgaggca
      421 tccatggtgc cgccggaggc cgcacccgcg ccgatcatgg tgtggatctc gtcgatgaac
      481 agcaccgcat tggggatcgc gctcaactcc ttcagcacgg atttgaggcg ctgctcgaag
      541 tcgccgcgat atttggtgcc cgccagcagc gagcccatat ccagcgaaaa gacctgagcg
      601 tccctgagta tctccggcac ctcgttctcc acgatgcgcc gcgccagacc ttcggcgatc
      661 gcggtcttgc ccacgcccgc ctcgccgacc agcagcgggt tgttcttgcg gcggcggcac
      721 agggtctgga tcacgcgctc gagttcgagg tcgcgcccga tcagcgggtc gatctttccg
      781 gccagcgcat gcgcattcag atccagcgtg tagttcttca gcacaccttc atcggcggcg
      841 ggctgttcgg cctcgccttc gacttgcggt tgagccgctt tgcccgagtc gcccttggtg
      901 atgccatgca cgatctggtt caccacgtcg atgcggttga cgccgcgttc ggtcaggaaa
      961 tgcacggcgt gcgaatcctt ttcgccgaac agggcggcca gaatattggc gccgaccact
     1021 tccttcttgt tggccgactg cacatgcagg atcgcgcgct ggatgacgcg ctgaaatccg
     1081 accgtgggct gagtgtccac ctcgccatcg ccgggaacga tgggggtgtg ggtattgatg
     1141 aacgcagaca cgacccggcg caggtcgtcg aggtcgacgt tgcaggagcg cagtatctgg
     1201 gccgccgagg ggttatccag catcgcgagc agcaggtgct ccacggtgat gagttcatgg
     1261 cgtttctggc gcgcctcgac aaaggccaag tgcaggctga cttccaattc ttgcgcgatc
     1321 atttcagatt tcctccgttc cgcatctgag cggatgcccg tgttgctttg catactcggt
     1381 cacttgcacg accttggtct ccgcgacatc gcgggaataa accccgcatg atgccgatcc
     1441 atcgttgtgt atcttgagca tagtctgcag cgcgcattcg cggttcattg cgaagaaacg
     1501 ctgcaaaaca tcgaccacaa aatccatcgt ggtgtaatcg tcgttgaaca aaaccacctt
     1561 gtacatcggg ggcggtttta ttttcgtctg ttccggcgcg agtaccgagt tgctttgttg
     1621 ttggtttgcc atggtggttc tatgttgggg attcttacat gaggttcgat tctcatgctc
     1681 aatgattagc gatgcttttt caagcctatt caaaaaatat tttttaaacc ctcttgacga
     1741 tgccggcaaa atgattgtaa aaagtacact ggtgtttgat tttaagctct ctgcagtact
     1801 ggttttgcca tgtacgaact tagattcaaa cggtcttgcg gggttttccc gtttttttgt
     1861 aaggaagtaa tcacatggca actggtacag tcaaatggtt caatgatgcg aagggttttg
     1921 gtttcatcac tccggatgat ggtagcgaag atttgtttgc acatttctct gcgatcaata
     1981 tgagtggttt caagtccctc aaagaaggcc agaaggtctc cttcgatctg gtgcaaggcc
     2041 caaagggtaa gcaagcatcc aatattcaag ccgcctgata cgcggttttt ttacgggtaa
     2101 aaaagcccgc cgtattccgg cgggcttttg tctttggtct cgccgatcta tttgcctaac
     2161 aggctcttga tccccatccc cacaccgact gccacgccgc cgaccgcacc gatggcggcc
     2221 gtcttggcca gcgagatcgc catcttggta ttcagttcgc cgatgatcgc gttgacgacc
     2281 tgagcggagg tcgcccctcc gttgcgcttg ccgatgttgt gcagttcgat gtccggcagg
     2341 gtcagattca gcatgccgtt gtagctgact ttgatgtcgc gaatgaccag agactcgatg
     2401 atcattttct tgctggtctt cttgtcggca ggcgatggtt tcttgtcgct gcccagatag
     2461 cgttcaacgt tgcgctggat cgcatggaag ttggtgccgc tgtcattctt ttcatagacg
     2521 atatcgggtg cggcgatcag tatcttgtgg atcaggatga cgtcatcggt aacggtggcc
     2581 gcctcgatgg cgacctcgat gtgatcgacc ttgaacgcgt gcgggctctt gaagcctttg
     2641 gggctgccca gcgacaggtc ggagatcgtg ccgcgtccat tgctcgcgga tagtttgacc
     2701 cggctgacgc tgacttgggt ctgggtcatt tccgagccga acttctcgat ggccgatttc
     2761 accagcccat taagcggatt gttgaaccag agatagaggg cgactgttgc gatggcaagc
     2821 aggatcagca atgcgatacg agattttttc acgatgtttt ctttgccgtt gaacgcgcca
     2881 gtttaacttt gcctgcagta aaaacccggc aggagccggg tctgttacag ggccatgtgg
     2941 cctgcggtct gtcgattgcg atcagggccg cctcagatgg cgtccagggc ggcgttgaac
     3001 gtcgcgcttg gacgcatcgc tttcgaggtc ttgtcgaagt ccgggtggta atagccgccc
     3061 atatcgaccg gcttgccctg tgccgcgatc aattcggcgt tgatcttcgc ttcgttgtcc
     3121 gtcaatacct tggctaacgg cgcgaacttg gctttcagtg ccgcatcctt gtcctgtgcg
     3181 gccaaagcct gcgcccagta cagggcgagg tagaagtggc tgccccggtt gtccagttcg
     3241 ccgatcttgc gggcgggcga cttgttgttc tccagtatct tgccgttggc ctgatccagc
     3301 gtgtccgcca gaacctgcgc cttggcgttc ttgaaggtct gcgccagatg ttccagcgag
     3361 acgcccagtg ccaggaactc gcccagcgaa tcccagcgca gataaccttc ctgctggaat
     3421 tgctgcacat gcttcggcgc cgaaccgccc gcgccggtct cgaacaatcc gccgccattc
     3481 atcagcggca cgatggacag catcttggcg ctggtgccca gttcgagtat cgggaacagg
     3541 tcggtgaggt agtcgcgcaa cacgttgccg gtgacggaga tggtgtctag tcctgcgcgg
     3601 atgcgatcca gcgaaaactg gatggcttct tccggcgtca tgatgctgat gtccaggccg
     3661 ctggtgtcgt ggtccttcag atagcgttcc actttacgga tgatctgcgc atcgtgcgca
     3721 cggttcttgt ccagccagaa tacggcaggc gtgttggaga ggcgggcacg ggtgaccgcc
     3781 agcttgaccc agtcctgaat cggcgcgtct ttcgcctggc acatgcggaa gatgtcgccc
     3841 tgttccacgt cctgctccag cagggtcttg ccggactcgt ccaccgcgcg gattttgccg
     3901 ttggccggag cctcgaaggt cttgttgtgc gagccgtact cttccgcctg ttgcgccatc
     3961 aggccgacgt tgggtaccga gcccatggtc ttggggtcga acgcgccgtg tttcttgcag
     4021 tcgtcgatga ccacctgata gatgcgcgca tagcaacggt cggggatcat cgccttggtg
     4081 tcgtgcagct tgccgtccgt gccccacatc ttgcccgagt cgcggatcat cgccggcatg
     4141 gacgcgtcga cgatgatgtc gctgggcaca tgcaggttgg tgatgccctt gtcggaattc
     4201 accatcgcca gttccgggcg ggactggtag caggcctgga tgtcggcttc gatctcggcg
     4261 cgctgatctt cgggcaagct ggcgattttg gcgatcaggt cgccgaagcc gttgttgacg
     4321 ttcacaccca gtttcttgat ggtcgccgcg tgtttctcga atacatcctt gaagtaaacc
     4381 gtgacggcgt ggccgaacat gatggggtcg gagattttca tcatggtggc cttcaggtgc
     4441 agagacagca gcacgccttg ttgcttcgcg tcctgcatct gcgcctcgta gaaatcgcgc
     4501 agcgcgcggc ggctcaagac acaagcgtcg atgatctcgc ctgccttgac gggagttttg
     4561 tccttgagca cggtgacatt gtcgtcttgg cctacgaatt cgattttgaa gcttccggct
     4621 tcggagacgg tgacggattt ttcgctgccg tagaaatccc cagtattcat gtgtgcgacg
     4681 tgggtcctgg agtcggagct ccatgcgccc atcttgtgcg ggttcttgcg cgcgaactgc
     4741 ttgaccgagg cggccgcgcg gcggtcggag ttgccttcgc gcagcacggg gttcaccgcg
     4801 ctgccgagca ccttgccgta gcgcgccttg atgtctttct cggcatccgt tttcggatca
     4861 tcgggataat ccgggatgtt gaaaccctgc gactgcaatt ccttgatcgc cgctttcaac
     4921 tggggcgacg atgcgctgac gttcggcagc ttgatgatgt tggcttccgg tttcagcgtc
     4981 agctcgccaa gctcggtcag ttcatcggcg atcttctggg cggcggtcag atgctcgggg
     5041 aagttcgcga gtatccgtcc cgcgagggag atatttcggg tttcaacggt aactccggct
     5101 gctttggtga acgcctgaac gatgggcagc aaagagtaag tcgccagtgc cggcgcttca
     5161 tcgaccatcg tgtaaataat ttttgggctc gtcatgtcgt tctcgtctcg taaaatttgg
     5221 atcatttagt taatgatcgg taactattta ttcgtatact tcacgtcact ggctggctaa
     5281 tgtcgccgtg cgctatccgc accgtttgcg gtgcgtcatc tgaaacttgt ttcaccgggg
     5341 gaaaaggggc aatgtctctt gctatcgcaa cggcgatagc ttcatagatc gctttatcca
     5401 gtggcagccg tgagtgaaac cgcaaacttt gaaaaaatga aatgagccgt atcctgctgt
     5461 tcaacaaacc ctatggtgtg atctgccaat tcagccggga cggactgcat ccgacgctgg
     5521 cggattatat cgtgcttccc gatttttatc cggccggacg gctggacacc gacagcgaag
     5581 gcttgttgct gctgaccgac gacggcaagt tgcaacaccg catcaccgat ccgaaacaca
     5641 agttgcccaa gacctattgg gtgcaggtgg aaggcgtgcc ggatgaggtt gcgctggaac
     5701 aattacggcg cggcgtacaa ctcaaggatg gcatgacgct gcccgccggg gcgcgcctga
     5761 tcgacgaacc ggcgatgcta tggccgcgca atccgccgat ccgtgaacgc aaagcgattc
     5821 cagccagctg gctggaactg accatacggg aaggacgcaa tcgtcaggtg cggcgcatga
     5881 ccgcagcagt caatttccct accttgcgtc tgatccgtta cgcgatcggc gactggacgc
     5941 tggacggcat cgcgccgggg cagtggcggc aggaagaggt gtgatgctgg cggggatatt
     6001 aaagggagat tgcgatgttg cgtaattcga ttttgtttgt tgcgttgttg tcgctaggga
     6061 tgcctgcaag cgcaggcacg ccccgcgaga tcgccgggaa accggcggcc aagctggtgg
     6121 ggacttggca cagtgcgcag gagacgatta ctttcaagcc caacggactc gtcgtctaca
     6181 agggacacag ccattacttc gccgcaggca acggggtgat ccagatcaag agcaagcatg
     6241 tcatccgcaa ccttccctac cggatgttcg atggcaaact gaccgtgacc gacaacggcg
     6301 tggacacggt ctatacccgg gagtaaatgg ttttgtggga gcggcttcag ccgcgattcc
     6361 gatggttaat ccaactgcct atcgcggctg aagccgctcc caccaagggg agtcgcttca
     6421 tcaacagtac aaccctaccc ttgcaggaat gccgtccatg cgacagtcga ggaatcagcc
     6481 gcgtccccgt ccgcgtggaa cacccgctgg ttttccggcc acgtgttcgc gccggacgcg
     6541 gggcgcaggc tggaacaagc ccggttgcgg catcgcctga tgacgcgcgg cttcagatag
     6601 tgcttgatcg ccggtctttt tcggttgcgg ctggcgtggc ttgccgggtt gtccgccgtt
     6661 gcccggctgt gctgtgcggg gctgctgacc gttgcgtgcc tgttgctcgc gcggttgacg
     6721 cggcgcttgg ccgtcgcggg gtttctgggc tgtacgcggt gcgctggcgg tagtgttgct
     6781 gtggcgtttc tgtccgtgtt gcgggcgcgg tgcgcgcggc gcatcggccg ggatgttggt
     6841 cggcggcacg aagctgtcta ttgccacttt cggaatctga tgtttgatca ggcgctcgat
     6901 gtttttgaga tgctggaact cttcgctgtc caccagcgag atcgccgcgc cgttgctgcc
     6961 cgcgcgaccg gtacggccga tgcggtgtac gtagtcttcg ggtacgttcg gcaattcgaa
     7021 gttcaccaca tggggcagca tgtcgatatc cagtccgcgc gcggcgatat cggtcgcaac
     7081 cagcaccggc agcgagccgt ccttgaattg cgccagcgcc ttggtgcgcg ccgattggct
     7141 cttgttgccg tggatcgcgg cggcg
//
LOCUS       DASUIV010000197         5107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_698272, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000197 DASUIV010000000
VERSION     DASUIV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698272"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFW59_07250"
     CDS             complement(<1..425)
                     /locus_tag="VFW59_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364049.1"
                     /translation="MFAKSVSTARNTGALLKANWVLVALALTAFAGSAWAVEAMPAES
                     AMPWWGWPLALFVTCFFLGIVAVPSGVGGGVLFVPIVGGFFPFHLDFVRGAGLLVALA
                     SALAAGPSLLRGGLANLRLALPLAVLASASSIAGAMIGLA"
     gene            complement(425..1342)
                     /locus_tag="VFW59_07255"
     CDS             complement(425..1342)
                     /locus_tag="VFW59_07255"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5364050.1"
                     /translation="MRPAFSVLFLTTLIGVGQGLFITLFLCELIAPGWLTHEFMIGGA
                     VLVLIFSGLGGISSVFHLGHPERAWRAMTMWRTSWLAREGIALPSFMAAVFAYGLAHW
                     TGFGNTLIIGTVGVLLAIALYVCTGMIYGAIKVLKEWAHPMTLVNFIVLGCASGTTLA
                     AAYASLAAPAYASPELVAPLAQAAIAWTVLGMLTRGASMWRNATLRPVSTLQTAIGIR
                     HPHIVQKSQGSMGGSFNTREFFHGKSPAFVRGVMVTMVLGAFVLPLFLVLAVPLLAFV
                     VQYVGLLAERWYFFAEAQHPQNLYYQGKS"
     gene            complement(1355..2077)
                     /gene="soeB"
                     /locus_tag="VFW59_07260"
     CDS             complement(1355..2077)
                     /gene="soeB"
                     /locus_tag="VFW59_07260"
                     /EC_number="1.8.5.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase subunit SoeB"
                     /protein_id="HEX5364051.1"
                     /translation="MTQLALVIDLNVCVGCHACVTNCKQWNTSGAAGSLTDCNPYEAE
                     PNGTFFNRVQTFEVGTFPQTETVHFPKSCLHCEDPACVPVCPTGASYKRKEDGIVLVD
                     YDKCIGCKYCSWACPYGVREFDEQRKVMTKCTLCVDRIYDQKLPEAERKPSCVLACPP
                     GARLFGDVHDPESEVSVAIRERGGYALMPEAGTKPSNHYLPRRKTTLSVKEDELYRSD
                     NPHKIDGMPFDRNLPPTTEELN"
     gene            complement(2074..4980)
                     /locus_tag="VFW59_07265"
     CDS             complement(2074..4980)
                     /locus_tag="VFW59_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX5364052.1"
                     /translation="MPSPIHDPVSSPLPDAITETRTTTCYMCACRCGIRVHLRDGEVR
                     YIEGNPDHPLNKGVICAKGGSGIMKQYSPARLTKPLRRKPGAERGANQFEEISWEETF
                     DILEKRLSHLRATDPKKFALFTGRDQMQALTGMFARQFGTPNYAAHGGFCSVNMAAGM
                     IYTIGGSFWEFGGPDLERAKVFYMIGTAEDHHSNPLKSAIGDFKRNGGRFVAINPVRS
                     GYAAIADEWIPIKPGTDGALILAIIREIIALGLYDREFLARYTNSGQLVNQDEGSPEF
                     GMMLRNPEGEKINPLRPHNFYWWDRLSDKPVADHTKDTDPVLFGSFTMPDGTKVKPAF
                     QLLKERVADCTPEWAAGITGIDAATIKRIAHEMGITARDQKIELPIPWTDWWGEEHET
                     VTGNPVAFHAMRGLAAHSNGFQTIRSLAILMSLLGTIDRPGGFRHKAPYPRAIPPNAR
                     PPKGPEAVQPNTPLNGAPLGWPESPDDLFVDAEGKPVRLDKAFSWEHPLSAHGLMHNA
                     ITNAWRGDPYPIDTLLLFMANMAWNSSMNTAEVRNMLVDKNEDGGYKIPFIVVCDAFQ
                     SETTAFADLILPDTTYLERHDCMSMLDRPISEFDGPVDAVRIPVVAPKGECKPFQDVL
                     VELGSRLKLPAFTTAEGGRKFKDYPDLVVNYETAPGSGIGFLTGWRGKDGDQAMRGEP
                     NPKQWEAYEKNGCFHQYHLPPSSQYMRNWNKEYMDWSQQAGLRRDNDPIIIHIYSEIL
                     QGFRLAAEGKRPGKQPPDELRERVATYFDPLPFWYAPLEDEQSDLKHYPLNALTQRPM
                     AMYHSWDSQNAWLRQIHTYNYLFVNTRTAEAAGISDGGWMWVESQWGKVRCLCRYSEA
                     VEPGTVWTWNAIGKSSGAWNLTGDANESKQGFLLNHLISEELPATANGKRFSNSDPIT
                     GQAAWYDVRVRIYPAEPGEPEESFPQFDTMEPVPGMEPRKMGWQAWFAGKGKNK"
BASE COUNT         1034 a         1587 c         1525 g          961 t
ORIGIN      
        1 gcgaggccga tcatggcacc cgcgatcgaa ctggccgaag ccagcaccgc cagcggaagt
       61 gccagtcgca gattggccag cccgccgcgc aacagcgacg ggccggctgc cagcgcgctt
      121 gccaatgcca ccagcaaacc cgcaccgcgc acgaagtcga gatggaatgg gaagaaaccg
      181 ccgacgatag gcacgaacag cacgccacca ccaacaccgg acggcaccgc gacgatgcca
      241 agaaagaaac aggtcacgaa caatgccaac ggccagcccc accaaggcat ggcgctctcg
      301 gccggcatgg cttcaacagc ccaagcgcta ccggcaaagg cggtcaacgc caatgccacc
      361 agcacccaat tggccttgag cagtgcgcca gtattccttg ctgttgaaac ggattttgca
      421 aacatcagga tttcccctgg taatacagat tctgcggatg ctgcgcctcg gcgaagaaat
      481 accagcgctc tgccagcaag ccgacatact gcactacaaa tgccagcaac ggcactgcca
      541 gcaccagaaa cagcggcaat acgaaggctc ccagcaccat cgtcaccatc acgccacgaa
      601 cgaaagcggg gctcttgcca tggaagaatt cgcgcgtgtt gaatgaaccg cccatggagc
      661 cctgtgattt ctgcacgata tgcggatgac ggatgccgat ggcggtctgc aaggtgctga
      721 ccgggcgcag tgtcgcattg cgccacatgg atgcacctcg ggtcagcatc cccagcacgg
      781 tccaggcaat tgcggcctga gccaatggtg ccaccagctc aggtgacgcg taagcaggcg
      841 cagcaaggct cgcataggct gctgcgagcg tcgtgccgga ggcgcaaccc agcacgatga
      901 aattcaccag cgtcattggg tgagcccact ccttcagcac cttgatcgca ccatagatca
      961 tgccggtaca gacatacaac gcgatcgcca gcaacacgcc gaccgtgccg atgatcagcg
     1021 tgttgccgaa acccgtccag tgcgcgaggc cataggcgaa caccgccgcc atgaaggatg
     1081 gcagggcgat gccttcacgc gccagccagg aagtgcgcca catcgtcatt gcgcgccagg
     1141 cgcgctccgg atgccccaga tggaacaccg aagagatacc acccaggccg ctgaatatca
     1201 gcaccagcac ggcgccgccg atcatgaatt catgggtcaa ccagccgggg gcgatcagtt
     1261 cgcacaggaa cagcgtgatg aacaggccct ggccgacacc gatcagggtg gtcaaaaaga
     1321 gtacggaaaa agctggacgc atgttgggta tgccttagtt gagttcttca gtggtcggcg
     1381 gcaagttgcg gtcgaacggc atgccatcga tcttgtgcgg attgtccgag cgatacagct
     1441 cgtcttcttt cacactcagg gtagtcttgc gacgcggcag ataatgattg gacggcttgg
     1501 tccccgcctc aggcatcagc gcatagccgc cgcgctcgcg gatcgcgacc gacacttcgg
     1561 actccggatc atgcacatcg ccgaacaggc gcgcgcccgg cggacaggcc agcacgcagg
     1621 aaggcttgcg ctctgcttcc ggcagtttct gatcgtagat gcggtccacg cacagcgtgc
     1681 acttggtcat gaccttacgc tgttcgtcga actcgcgcac gccgtacgga caggcccagg
     1741 agcaatactt gcaaccgatg catttgtcgt aatcgaccag cacgatgccg tcttccttgc
     1801 gcttgtagga tgcaccggtc gggcacaccg gcacgcaggc cgggtcttcg caatgcagac
     1861 aggatttcgg gaaatgtacc gtctccgtct gcgggaaggt accgacctcg aaggtctgga
     1921 cgcgattgaa gaaggtgccg ttcggctcgg cctcataggg attgcagtcg gtcaacgagc
     1981 cggccgcgcc ggaggtattc cactgcttgc aattggtcac gcaggcatga caacccacgc
     2041 agacgttcag atcaatgacg agtgctaatt gagtcatttg ttttttcctt taccggcaaa
     2101 ccacgcctgc cagcccattt tccttggttc catgcccggc accggctcca tggtgtcaaa
     2161 ctgcgggaag ctctcctccg gctcgcccgg ttccgcagga tagatgcgca cgcgtacgtc
     2221 gtaccaggct gcttgcccgg tgatcgggtc ggagttggaa aaacgcttgc cgttcgccgt
     2281 cgcaggcagc tcttccgaga tcaggtgatt gagcaggaaa ccctgtttcg attcattggc
     2341 atctccggtc aggttccacg caccggagga cttgccgatc gcgttccagg tccacaccgt
     2401 gcccggctcg accgcctcgg aataacggca gagacagcgc accttgcccc actgcgactc
     2461 cacccacatc cagccgccgt cactgatgcc tgccgcttct gcggtgcgcg tgttgacgaa
     2521 cagatagttg taggtatgaa tctggcgcag ccaggcgttc tgcgaatccc acgagtggta
     2581 catcgccatc gggcgctgag tcagcgcatt cagcggatag tgtttcagat cgctctgctc
     2641 atcttccagc ggcgcatacc agaacggcag cggatcgaaa taagtggcga cgcgttcacg
     2701 caactcatcc ggcggctgct tgcccggacg cttgccctct gccgcgagac ggaagccctg
     2761 cagaatctcg gaatagatat ggatgatgat cggatcgttg tcgcggcgca gaccggcctg
     2821 ttgcgaccag tccatgtact ccttgttcca gttgcgcatg tactggctcg aaggtggcaa
     2881 gtgatactgg tggaaacagc cgttcttctc gtaggcttcc cattgcttcg gattcggctc
     2941 gccgcgcatg gcctgatcgc catccttgcc gcgccagccc gtcaaaaagc cgataccgga
     3001 gcccggtgct gtctcgtagt tgaccaccag atcggggtag tccttgaact tgcgaccgcc
     3061 ttcagccgta gtgaatgcag gcaacttgag gcgactgccc aactcgacca gcacgtcctg
     3121 gaacggcttg cactcaccct tgggcgcaac caccggaatg cgcaccgcat ccaccgggcc
     3181 gtcgaactcg gagatcggac ggtcgagcat ggacatgcaa tcgtgccgct cgagataggt
     3241 cgtgtccggc aggatcagat cggcgaatgc ggtggtttcc gactggaacg cgtcgcacac
     3301 tacgatgaat ggaatcttgt aaccaccgtc ttcattcttg tcgacgagca tgttgcgcac
     3361 ttccgccgtg ttcatgctgg agttccatgc catattcgcc atgaacagca gcaacgtatc
     3421 gatcgggtaa ggatcgccgc gccatgcatt ggtgatggcg ttgtgcatca ggccgtgggc
     3481 cgacagcgga tgctcccagg aaaatgcctt gtccagacgc accggcttgc cctcggcatc
     3541 cacgaacagg tcatccggac tctccggcca gcccagcggc gcaccgttca gcggggtatt
     3601 cggctgcacg gcttccgggc ctttcggcgg gcgggcattc gggggaatcg cacgcgggta
     3661 cggcgccttg tggcggaagc cgccgggcct gtcaatcgtg cccagcagcg acatcaggat
     3721 cgccagcgag cgtatggtct gaaagccgtt ggagtgcgca gccagaccgc gcatcgcatg
     3781 gaaggcgacc ggattgccgg tgacggtttc gtgctcttcg ccccaccagt cggtccaggg
     3841 aatcggcaac tcgatcttct ggtcgcgtgc ggtgatgccc atctcatgcg cgatgcgctt
     3901 gatggtcgcc gcatcgatgc cggtaatccc ggcagcccat tccggcgtgc aatcggctac
     3961 gcgctctttc agcaactgga acgcgggctt gacctttgtt ccgtccggca tggtaaagct
     4021 gccgaacaac accggatcgg tatccttggt gtggtcggcc accggcttgt cgctcaggcg
     4081 atcccaccag tagaaattgt gcggacgcag cggattgatc ttctctccct ccggattgcg
     4141 cagcatcatg ccgaattcgg gactaccctc gtcctgattc accagctggc cggagttggt
     4201 gtagcgcgcg aggaactcgc ggtcgtacag ccccagcgcg atgatctcgc ggatgatcgc
     4261 caggatcagc gcgccgtcgg tgcccggctt gatcggaatc cactcgtcgg caatcgcggc
     4321 ataaccgctg cgcaccggat tgatcgcgac gaagcgtccg ccgttgcgct tgaagtcgcc
     4381 gatcgcactc ttcagcggat tggagtgatg atcctcggcg gtaccgatca tgtagaaaac
     4441 tttggcgcgc tccagatcgg ggccgccgaa ttcccagaac gaaccgccga tggtgtagat
     4501 catgcctgcg gccatgttca ccgagcagaa gccgccgtgc gcggcatagt tgggtgtgcc
     4561 gaactggcgc gcgaacatgc cggtcagcgc ctgcatctgg tcgcgtccgg tgaacagcgc
     4621 aaacttcttc ggatcggtgg cgcgcagatg cgacaggcgc ttttcgagga tatcgaaggt
     4681 ctcttcccag ctgatttcct cgaactggtt cgcgccgcgc tcagctccgg gcttgcggcg
     4741 cagaggcttg gtcaggcgcg ccggggaata ttgcttcatg atgcccgaac cgcccttggc
     4801 acagatcacg cccttgttca gcggatggtc gggattgccc tcgatatagc gcacctcgcc
     4861 gtcgcgcaga tgcacgcgga tgccgcagcg gcaggcgcac atataacagg tggtcgtacg
     4921 cgtctcagtg attgcgtcgg gcagcggaga tgaaaccggg tcgtgtatgg gcgatggcat
     4981 aatctgtcgt cgttgaattt gcatatcgca gcaatagcgc ggaaagccca cgctgcgagc
     5041 gtttaaaacc tcgccattta ggcgcaatcg cttgcccgac gtctataccc acatcgggtg
     5101 agacggc
//
LOCUS       DASUIV010000198         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_46359, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000198 DASUIV010000000
VERSION     DASUIV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46359"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..430
                     /locus_tag="VFW59_07270"
     CDS             158..430
                     /locus_tag="VFW59_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364053.1"
                     /translation="MPISRVNFFVRKHMSHIEMVGVLMRIFSFSLVSWLGPESPFLFV
                     WIFNTTDAVILSWCSVLKKDWAYTMLNMFWIMVGVVGIFRATHFIH"
     gene            573..1715
                     /locus_tag="VFW59_07275"
     CDS             573..1715
                     /locus_tag="VFW59_07275"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX5364054.1"
                     /translation="MNASTNSIGIVSAKRASFDTPLVFRSGAVLPRYELVYETYGKLN
                     ADKSNAVLICHALSGHHHVAGQYADQPKNVGWWDNMVGPGKPINTDKFFVIGLNNLGG
                     CHGSTGPSSVNPETGKPYGASFPVITVEDWVESQARLADQFGIRQFAAVIGGSLGGMQ
                     ALQWSLAHPERVRHVLAIASAPRLTAQNIAFNDVARNAILTDPDFHGGDYYQHGVVPT
                     RGLRLARMLGHITYLSDDAMADKFGRELRTDKFNYGYDIEFEIESYLRYQGDKFASYF
                     DANTYLLMTKALDYFDPSHDFGGDLNKAFARAKSDFLVISFTTDWRFSPQRSREIVRP
                     LLHNRRNVSYAEITSTHGHDSFLMQHEHYFDVMRAYLDNVASEFGK"
     gene            1718..2332
                     /gene="metW"
                     /locus_tag="VFW59_07280"
     CDS             1718..2332
                     /gene="metW"
                     /locus_tag="VFW59_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX5364055.1"
                     /translation="MRQNDITRLAAERPDFAAIAAWIPKGASVLDLGCGDGSLLRYLK
                     ETRAVRGYGVEISDLDIVSCIANGVNVIQNDLDSGLSDFEDNSFDFVMLSQTLQATRH
                     TEALIREMLRVGREGIVSFPNFAYWKNRLNVLLGNMPVSSELPYQWYDTPNVHLCTLH
                     DFESFCRAQHVTVLERRVMTGEREVSLLPNLLGSTAVYRFQRAV"
     gene            2450..3709
                     /locus_tag="VFW59_07285"
     CDS             2450..3709
                     /locus_tag="VFW59_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEX5364056.1"
                     /translation="MTVWQQLFTRRMLICVFVGFSSGLPLYLLFNLVPAWLRSEHVDL
                     KTIGLFALIQFPYTWKFLWSPLLDRYVVPAFGRRRGWMLLTQIGLLAVIAAMGGFSPQ
                     SDLRTIAWVATLLAFLSATQDIVLDAYRRELLSDAELGLGNSVHVNAYRIAGLVPGSL
                     SLILADFLPWDMVFIVTALFMLPGVVMTLLVSEPHRASPPKTLREAVVEPFHEFITRS
                     GWNSALLILAFLFFYKLGDSMCTALATPFYLDMGFSKTQIGLIAKNAGLWPAVIGGLL
                     GGLWMVKIGINRALWLFGVVQVLSIFGFAWLASIGHHAEITSVELAQLAVVIGLEALG
                     VGLGTAAFVAFIARTTHPAYTATQFALFTSLAAMPRTFANAATGWLVEAMGWMGFFLL
                     CAVLAVPGMLLLLKVAPWGEKQPDSGA"
     gene            3788..>4322
                     /locus_tag="VFW59_07290"
     CDS             3788..>4322
                     /locus_tag="VFW59_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364057.1"
                     /translation="MGDLGEARCGEKTEALDSVFDLLPGIVSHVDFLAPTAHAQKRAI
                     AFDVGGAERDCCTQFGFYGSAAVFDIPVGQQRNAHFHIAAQIGRIAPEGQIAYRFVRP
                     HPLEQGFMDVPGFRIVNEIPQRLLADVPAGHALPVIGCGEQVPLIVEIDQQIVLVYLI
                     NDFKRITWHSLAPCQSGG"
BASE COUNT          784 a         1352 c         1299 g          887 t
ORIGIN      
        1 ccgagcgtca agcagcgcgt gctgaccacc agtctgccgg aactcaccac gctcacgcag
       61 aagattttgc gtgccgacga cccgctgcgc atccgcgagt tgctggacaa gctgaatgcc
      121 tgagccgtta aactagcggg aaacggacac ttttcaaatg ccaattagtc gcgttaattt
      181 tttcgtgcgc aagcacatgt cgcatatcga aatggtcggc gtgctgatgc gcatcttcag
      241 cttttcgctg gtcagttggc tcggcccgga aagccctttc ctgttcgtgt ggatattcaa
      301 cacgaccgac gccgtcatcc tttcctggtg ttcggtgctg aagaaagact gggcctacac
      361 catgctcaac atgttctgga tcatggtcgg cgtggtcggt atcttccgcg cgacccactt
      421 cattcattga caggttttcg caaaacacgg aaactaccag ccattcagtg ccgatttgac
      481 atcagcttgc ggggcactta aaacattcca gctaaagcga ggcaacccgc tccgcgcaag
      541 ctgaacgggt tttgtttttg gggaagagca atttgaacgc atcgacaaat agcataggta
      601 tcgtcagcgc aaagcgcgcg tcgttcgaca ctccgctggt gtttcgcagc ggtgcggtgt
      661 tgccgcgcta cgagctggtg tatgaaacct acggcaagct taacgccgac aaatccaacg
      721 cagtcctgat ctgccacgcg ctgtccgggc atcaccatgt tgcgggccaa tacgccgacc
      781 agccgaagaa cgtcggctgg tgggacaaca tggtcgggcc gggcaagccg atcaacaccg
      841 acaaattctt cgtgatcggg ctgaacaacc tcggcggctg ccacggctcg accgggcctt
      901 catccgtcaa tccggaaacc ggcaagcctt acggcgcgag cttcccggtc atcaccgtgg
      961 aagactgggt cgagtcgcag gcgcggctgg ccgaccagtt cggcatccgg caattcgcgg
     1021 cggtgatcgg cggcagcctg gggggcatgc aggcgctgca atggtcgctg gcgcaccccg
     1081 agcgcgtgcg ccatgtgctg gcgatcgcca gcgcgccgcg cctcaccgcg cagaacatcg
     1141 cgttcaacga tgtcgcgcgc aacgcgatcc tgaccgatcc agattttcac ggcggcgatt
     1201 attaccagca cggcgtcgtg ccgacgcgcg gcctgcggct ggcgcgcatg ctgggacaca
     1261 tcacctacct gtccgacgac gcgatggcgg acaaattcgg gcgcgaattg cgcaccgaca
     1321 aattcaatta cggctacgac atcgagttcg agatcgaatc gtacttgcgc tatcagggcg
     1381 ataagtttgc gtcctacttc gacgcgaaca cctacctgct gatgaccaag gcgctggatt
     1441 acttcgaccc gtcgcacgat ttcggcggcg acctgaacaa ggccttcgcg cgcgccaaat
     1501 cggatttcct ggtgatctcg ttcaccaccg actggcgctt ctcgccgcag cgctcgcgcg
     1561 agatcgtgcg gccgctgctg cataaccggc gcaacgtcag ctacgcggaa atcacttcga
     1621 cgcacggcca cgactccttc ctgatgcagc acgagcatta cttcgacgtg atgcgcgctt
     1681 acctcgataa cgtcgccagc gagttcggca aatgagcatg agacagaacg acatcaccag
     1741 actcgccgcc gagcggcccg acttcgccgc catcgccgca tggataccca agggggcctc
     1801 ggtgctcgac ctcggttgcg gcgacggcag cctgctgcgc tacctcaagg aaacccgcgc
     1861 ggtgcgcggc tacggcgtgg agatcagcga cctcgacatc gtgtcgtgca tcgccaacgg
     1921 cgtgaacgtg atccagaacg acctcgactc cggcctgtcc gatttcgagg acaactcgtt
     1981 cgacttcgtg atgctgtcgc agacgctgca agccacgcgc cacaccgagg cattgatccg
     2041 cgagatgctg cgcgtcgggc gcgagggcat cgtcagcttc cccaacttcg cctactggaa
     2101 gaaccgcctc aacgtgctgc tcggcaacat gccggtgtcc agcgagctgc cctaccagtg
     2161 gtacgacacg cccaacgtcc acctgtgcac gctgcacgac ttcgagagct tctgccgcgc
     2221 ccagcatgtc acggtgctgg agcgccgcgt gatgaccggc gaaagagaag tgagcctgct
     2281 gcccaacctg ctcggcagca cggcggtgta ccggttccag cgtgcggtat aagcccgcca
     2341 ataccctctc cctagccctc ccccgcaagc gggggagggg acagagtctc ccctctccca
     2401 ctggtgggag aggggcaggg ggagagggcg cactcaaagg taaaatcaat tgaccgtctg
     2461 gcaacaactc ttcacccgcc gcatgctgat ctgcgtgttc gtcgggttct cttccggtct
     2521 gccgctgtac ctgctgttca acctcgtgcc tgcctggctg cgcagcgagc atgtcgatct
     2581 gaagaccatc ggcctgttcg cgctgatcca gtttccctat acctggaagt tcctgtggtc
     2641 gccgctgctt gaccgctacg tcgtgcccgc gttcggcagg cggcgcggct ggatgctgct
     2701 gacgcagatc ggcctgctgg cggtgatcgc cgcgatgggc gggttctcgc cgcagagcga
     2761 cctgcgcacc atcgcgtggg tcgcgacgct gctcgccttc ctcagcgcca cgcaggacat
     2821 cgtgctggat gcctaccgcc gcgagctgtt gtctgacgct gagctgggcc tgggcaattc
     2881 ggtgcatgtg aacgcctacc gcatcgcggg tctggtaccg ggctcgctgt cgctgatcct
     2941 cgccgacttc ctgccgtggg atatggtgtt catcgtcacc gcgctgttca tgctgcccgg
     3001 cgtggtgatg accttgctgg tgagcgaacc gcaccgtgcc tcgccgccca agaccttgcg
     3061 tgaagcggtg gtcgaaccgt tccacgaatt catcacgcgc agcggttgga acagtgcgct
     3121 gctgatcctc gcgttcctgt ttttctacaa gctcggcgac agcatgtgta ccgcgctggc
     3181 cacgccgttc tatctggaca tgggcttcag caaaacgcag atcggcctga tcgccaagaa
     3241 cgccgggctg tggcccgccg tgatcggcgg attactgggc ggcctgtgga tggtgaagat
     3301 cggcatcaac cgcgcgctgt ggctgttcgg cgtggtgcag gtgctgtcga tcttcggctt
     3361 cgcatggctg gcgtccatcg gccatcacgc cgaaatcacc agcgtcgaac tcgcgcagct
     3421 cgccgttgtg atcggcctcg aagcgctggg cgtggggctg ggcaccgcag ccttcgtcgc
     3481 cttcatcgcg cggaccaccc accccgccta caccgccacc cagttcgcgc tgttcaccag
     3541 tcttgccgcg atgccgcgca ccttcgccaa tgctgcgacc ggctggctgg tcgaggcgat
     3601 gggctggatg ggattcttcc tgttgtgcgc ggtgctggca gtgccgggga tgctgctgtt
     3661 gctgaaggtc gcgccgtggg gcgagaaaca gcctgattcg ggggcataac gggcgagaaa
     3721 tcgtttgccc tttttgttat ttgccgtcat ccttttccag gttgctcagg catgagtcgc
     3781 acatcgtatg ggagatttgg gggaggcgcg ctgcggagaa aagaccgagg cgcttgatag
     3841 cgtcttcgac ctcctgccag gaattgtttc ccacgtcgat tttcttgcac caactgcaca
     3901 tgcgcagaaa cgtgccatcg ctttcgatgt cggcggcgcc gagcgcgatt gctgcacgca
     3961 attcggtttt tacggttcgg cagcggtatt cgacattccg gtcgggcagc agcgaaatgc
     4021 tcatttccat atagcggcgc aaatcgggcg catcgcaccg gaagggcaga tcgcgtatcg
     4081 gtttgttcgc ccgcacccgc ttgagcaggg tttcatggat gtgccgggtt tccgcatcgt
     4141 gaatgaaatc ccacagcgat tgcttgccga tgttccggct ggacatgcgc tgcccgtcat
     4201 tggctgcggc gaacaagtcc cactgatcgt tgaaatagac cagcagatcg tcttggtcta
     4261 tctgataaac gacttcaagc ggattacttg gcatagcctt gctccctgtc agtctggtgg
     4321 gt
//
LOCUS       DASUIV010000199         7415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_975811, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000199 DASUIV010000000
VERSION     DASUIV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975811"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="VFW59_07295"
     CDS             complement(<1..1577)
                     /locus_tag="VFW59_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5364058.1"
                     /translation="MSSFKYLVVGSLLLTACAHSPQQVVPAVSAAPAEIAPVLPNIEL
                     SDELLYEYLLTEIASQRGHHALALEGSADLVRKTRDPRLARRAAQLAVESGQMDKAVD
                     AFRQWQELEPNATLAGHLLSSILLRGGRLDEAKQEFAKQLKASEPNFATEFTLVFQQV
                     MSYPDKAAALKLMRELAQPYPQVAEARWAVAYLAHRAGDDELALAEVQQARSLRPEWE
                     VPVLLEAQLLLKKEPQQALDRMHRYLSSYPDAREIRLQYARALLEQKQYQSARTEFQR
                     LAQESPDNPDMAFAIALISLQLKDFKGAEEQLRQALGEGKKDQDRVQYYLGQLSEAKE
                     DVDGALEHYRAVVDGEYRYAAQLRAVYLLNVRDGLDPARAYLQQIRIDNNQQRVQLLL
                     VETQLLREAKRFAEAYQVLQQGLEQLPNHPDLLYEAAMLADKIDKHDVSEKLLRKLIQ
                     LQPNHAHAYNGLGYSLLERNERVVEALGLVEKALKLAPDDAAIMDSVGWGYYRNGRLD
                     DSVNMLRRAFASNPDPE"
     gene            1654..2454
                     /locus_tag="VFW59_07300"
     CDS             1654..2454
                     /locus_tag="VFW59_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5364059.1"
                     /translation="MSDISPEITLSAQNLTRSFGKQQIIHDVSLTLRRGEVLGLLGHN
                     GAGKSTTLQMLTGCLQPDSGKIEICGIDLLQQPVLAKRHLGYLPEIPPLYRELCVNDY
                     LGFAARLRGIPTGDVAAALMQTKQRCGLEAVGGKIIATLSKGYQQRVGIAQAIIHNPA
                     VIVLDEPTVGLDPAQIRDIRTLIRDLGDSSSVILSTHLLGEVESLCDRVEIMQKGKLI
                     YGDTTARLANYGGEPNQLEANFIRITEQATAAHANMNVTTPDAETAEK"
     gene            2451..3194
                     /locus_tag="VFW59_07305"
     CDS             2451..3194
                     /locus_tag="VFW59_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5364060.1"
                     /translation="MIRLIAMRELRSLFTLPSSWFIFAVLQFIFAWFFLARLEAFLEI
                     QPQLAQLANPPGVTITIAAPMFNTIALLLMMLTPMYTMRLIAEERRNQTLQLLLSAPL
                     SDRHIVLGKFLGMLLFMIALTISVPVMLYSLALGTSLDHGLMLSNAIGLLLLTASFAA
                     LGLYVSALTSQPVIAAIGALAAQVGLWLADIGASAPDSPWHALSPLGHFQNFNAGLLS
                     SSDILFFVLFTGGFLLLTIRRLHNNRIYG"
     gene            3198..4544
                     /locus_tag="VFW59_07310"
     CDS             3198..4544
                     /locus_tag="VFW59_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX5364061.1"
                     /translation="MLRKFLTAPSLRNILFVLLLLGTIIGLSQLSSRHNWQRDITLNA
                     SNSLEPSSVTVLGQMTGPLDITVYATEHDPRLGDIRKLIREFVSLYQRYKPDLKLTFI
                     DPEKEPEKARDARIQLNGEMVVSYAGRSEHLTQINEQVLTSTLLRLAHTRDQTVMYLD
                     GHGERKLDGIANHDLGELFGAKLKQNGFRINSLNLALAQEVPDNTSVLVITQPQVDLL
                     PGEMDKLLRYVARGGNLLWLLDAEPLRGLEPLAEKLDLLLPPGIVIDPSATGMNVPVT
                     WSLGAAYPPHAITHDFNLITAFPSARPLAWNESSDWERHVLLEVAPRGWVSRYPAQNK
                     PFDKLHDTPGPAVIAAALQRHINDREQRIVVVGSGAFLANSFAGNGGNVDLGVNMVNW
                     LANEDSLITLQPRAAKDSNLTLNRTQLNVISFGFLIGLPLLLAGVGVRMWWKRRRA"
     gene            4705..5529
                     /gene="mutM"
                     /locus_tag="VFW59_07315"
     CDS             4705..5529
                     /gene="mutM"
                     /locus_tag="VFW59_07315"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX5364062.1"
                     /translation="MPELPEVETTRRGLEPHLVGATVTDVVVRNARLRWPIPDELPLR
                     GRAIRALHRRAKYLLVEFEHGTLILHLGMSGSLRIQPVGTPAEKHDHFDLVLANGTLM
                     RLRDPRRFGAVLWHEGDIHSHPLLAALGPEPLEQGPQQSAFDAAHLYRATRDRSVSIK
                     QCIMDNHIVVGVGNIYANEALFRAGIKPRLAAGKLSRERCARLVEAIRATLTEAIAQG
                     GSTLRDFVNASGQPGYFQQHYWVYGRAGESCRRCGTVIRQIKQGQRSSFYCATCQR"
     gene            5603..6472
                     /locus_tag="VFW59_07320"
     CDS             5603..6472
                     /locus_tag="VFW59_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5364063.1"
                     /translation="MNAKQEHLELGQFIPPVYHYNMLTNPERMAAFRTAIELAVPVGA
                     KVLELGGGTAVLSFFAAQRASKVWCVEKNPEMVREARRILALNPGSERVELVEADAYD
                     YLPPEPVDVVICEMLHVGMLREKQIPVIESFKRNYLAKFGAPLPRFIPEAFIQAVQPL
                     QQSFDFDGYYAPSTFFQNPSEIQPRSEELGKPAIYQMAIYEAPLPADCNWRGEIVIEQ
                     DGTLNALRFITKNLIAIDEAAQRTVDWFGHYLVTPLSDPLSVTAGQRLAVCFDYRPGD
                     PISALRPSVTPIT"
     gene            6632..>7415
                     /locus_tag="VFW59_07325"
     CDS             6632..>7415
                     /locus_tag="VFW59_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5364064.1"
                     /translation="MSEKISLSNDVTFAGLKLAEPILRAIADAGYTNPTPVQAQAIPQ
                     VLAGGDLLAGAQTGTGKTAGFTLPVLHRLSEKPAANPRAGAPRCLILTPTRELAAQVE
                     ESVQTYGKYLPLKSMVMFGGVNINPQIKALKSSVDILVATPGRLLDHVGQKTLDLSSV
                     EILILDEADRMLDMGFIRDIKKILALLPKQRQNLLFSATFSDEIKTLADGLLHNPGYV
                     EVARRNTTSELVEQSVHLIPQKLKSYLLAHLIKHNDWKQVLVF"
BASE COUNT         1535 a         2490 c         2026 g         1364 t
ORIGIN      
        1 atctcgggat cgggattgct ggcaaaggcg cgacgcagca tgttaacgct gtcatccagg
       61 cgcccgttgc ggtaataacc ccaacccacg ctatccatga tggccgcatc atccggcgca
      121 agcttcagcg ccttttccac caggccgagc gcttccacca cgcgttcgtt gcgctccagc
      181 aggctgtatc ccagcccatt gtaggcatgg gcatgattcg gctggagctg gatcagcttg
      241 cgcaacagct tttcggaaac atcatgctta tcgatcttgt ccgccagcat ggcggcctcg
      301 tagagcagat cgggatgatt gggcagctgc tccaggcctt gctgcaacac ctgataggcc
      361 tctgcgaatc gctttgcttc gcgcagcaac tgcgtctcga ccagcagcaa ctgaacacgc
      421 tgctggttat tgtcgatgcg tatctgttgc agataagcgc gcgccgggtc cagaccatcg
      481 cgcacgttca gcagatagac cgcgcgcagt tgcgccgcat agcgatattc gccatccacc
      541 accgcgcggt aatgctccag cgccccatcg acatcctctt tcgcctcgct caactgaccg
      601 aggtaatact gcacccgatc ctgatccttc ttgccctcgc cgagcgcctg ccgcagctgc
      661 tcctccgccc ccttgaagtc cttcaactgc agggagatca acgcgatcgc aaaggccatg
      721 tccggattgt ccggactctc ctgcgccaga cgctgaaatt cagtccgcgc ggattggtat
      781 tgtttctgct ccagcaaggc gcgggcatac tgcaatcgaa tctcgcgcgc atcgggataa
      841 ctcgacagat agcggtgcat cctgtccagc gcctgttgcg gctctttctt caacaacaac
      901 tgcgcctcca gcaaaaccgg cacctcccac tccggacgca gactgcgcgc ctgctgcacc
      961 tcggcgagtg ccagctcgtc atcgccggca cggtgtgcca gataggccac ggcccagcga
     1021 gcctcggcca cttgcgggta aggctgcgcc aattcgcgca tcagcttcag cgcggccgcc
     1081 ttgtccggat aggacatgac ctgctgaaac accagagtga attcggttgc gaaattcggc
     1141 tcgctcgcct tcaactgctt ggcgaactcc tgcttcgcct cgtccagccg tccgccgcgc
     1201 aacaggatcg aggacagcaa atgtccggcc agcgtcgcgt tcggctctaa ttcctgccat
     1261 tgccggaatg catcgacggc cttatccatc tgcccggact cgacggccaa ctgcgctgcg
     1321 cgccttgcca gacgcgggtc gcgcgtcttg cgcaccagat cggcgctccc ctccagcgcc
     1381 aacgcatgat gaccgcgctg actggcgatc tcggtcaaca gatattcata aagcaactcg
     1441 tcgctcaatt cgatattggg caacacaggt gcgatctctg ccggcgcggc tgatacagcc
     1501 gggacgacct gctgcgggga atgtgcacag gctgtgagca gcaaactgcc gacaaccaga
     1561 tatttaaatg agctcatgtc actactgctt ttgatgcggg cgcacatatt aggttatatt
     1621 tagccgtacc cacaaccaag acaatccgct accttgtccg atatctcacc cgaaatcacc
     1681 ctttccgcgc aaaacctgac gcgcagcttc ggcaaacagc aaatcatcca cgacgtctcg
     1741 ctgacattgc gacgcggcga ggtgctgggt ttgctgggcc acaacggcgc aggcaagagc
     1801 acgacattac agatgctgac cggctgcctg caaccggaca gcggcaagat cgagatctgc
     1861 ggcatcgacc tgctgcaaca acctgtgctg gcgaagcggc atctgggcta tctgccggag
     1921 attccgccgc tgtatcgcga actctgcgtc aatgactacc tgggcttcgc cgcgcgcctg
     1981 cgcggcatcc cgaccggcga tgtcgccgcc gcgctgatgc agaccaagca gcgttgcgga
     2041 ctggaggccg tgggcggcaa gatcatcgcg acgctatcca agggttacca gcagcgcgtc
     2101 ggcatcgcgc aagccatcat ccacaatcct gcggtgatcg tgctggatga acccaccgtt
     2161 ggcctcgacc cggcacagat tcgcgacatc cgcaccctga tccgcgatct gggcgacagc
     2221 agcagcgtga tcctctccac ccacttgctg ggcgaagtcg aaagcctgtg cgaccgggtg
     2281 gagatcatgc aaaagggcaa actgatctac ggcgacacca cggcacggct ggcgaattac
     2341 ggcggcgagc caaaccagct ggaagcgaat ttcatccgca tcacggaaca ggcaaccgcc
     2401 gctcacgcca acatgaacgt cacgacacct gacgcggaga ccgccgaaaa atgattcgtc
     2461 taatcgccat gcgcgaactg cgcagcctgt tcaccctgcc gtcttcctgg ttcatcttcg
     2521 cggtgctgca attcatcttc gcctggttct tcctggcgcg actggaggcc tttctcgaaa
     2581 tacaaccgca actggcacaa ctcgccaatc cgcccggcgt gacgatcacc atcgccgcgc
     2641 cgatgttcaa caccatcgcg ctgctgctga tgatgctgac accgatgtac accatgcgcc
     2701 tgatcgccga agagcgccgc aaccagacgc tgcaactgct gctgtcggcg ccgctgtccg
     2761 atcgccacat cgtgctcggc aaattcctcg gcatgctgct cttcatgatc gcgctgacca
     2821 tcagcgtgcc ggtgatgctg tacagcctgg cgctcggcac ctcgctcgat cacggcctga
     2881 tgctctccaa cgcgatcggt ctgctgctgc tcaccgccag cttcgcagcg ctggggctgt
     2941 atgtctccgc gctcaccagc cagccggtga tcgccgccat cggtgcattg gccgcgcaag
     3001 tcggcctgtg gctggccgat atcggtgcct cggcgcctga ttcgccctgg cacgcgctct
     3061 cccctctggg gcattttcag aacttcaatg ccggcctgct gagcagcagc gacatcctgt
     3121 tcttcgtgct gttcaccggc ggattcctgc tgctgacgat acgtcgcctg cacaacaacc
     3181 gcatatacgg ataaaccatg ctgcgtaaat tcctcaccgc tccatcgctg cgcaacatcc
     3241 tgttcgtgct attgctgctc ggcaccatca tcggcctgag ccaactctcc tcgcgccaca
     3301 actggcagcg cgacatcacg ctcaacgcca gcaacagcct ggagccatcc agcgtgacgg
     3361 tgctcggcca gatgaccggg ccgctcgaca taaccgtgta cgccaccgaa cacgaccctc
     3421 gtctgggcga catacgcaaa ctcatccgcg aattcgtctc gctctatcag cgttacaaac
     3481 ccgacctcaa actcaccttc atcgacccgg aaaaagaacc cgaaaaagcg cgcgatgccc
     3541 gcatccagct caatggcgaa atggtggtga gctacgccgg acgcagcgaa catctgaccc
     3601 agatcaacga acaggtgctt accagcaccc tgctgcgcct ggctcacacc cgcgaccaga
     3661 ccgtgatgta tctggacggg catggcgaac gcaaactgga cggcatcgcc aaccacgacc
     3721 tgggcgagct gttcggtgcc aagctcaaac agaacggctt ccgcatcaac agcctgaatc
     3781 tggcactggc acaggaagtc cccgacaaca ccagcgtact ggtcatcacc cagccgcagg
     3841 tcgatctgtt gcccggcgaa atggacaaac tgctgcgcta tgtggctcgc ggcggcaacc
     3901 tgctgtggct gctggatgcc gaaccgctgc gcggactgga accgctggcc gaaaaactcg
     3961 acctgctgct gccgccgggc atcgtgatcg acccttctgc gaccgggatg aacgtcccgg
     4021 tcacctggtc gctgggcgct gcctatccgc cccacgccat cacccacgac ttcaatctga
     4081 tcaccgcctt cccctccgcc cgcccgctgg catggaacga aagctccgat tgggaacgcc
     4141 atgtgctgct ggaagtcgcc ccgcgcggct gggtcagccg ctatcccgca cagaataagc
     4201 cgttcgacaa actgcacgac acgccgggcc ctgcggtcat cgccgcagca ctgcaacgcc
     4261 acatcaacga ccgcgaacaa cgcatcgtcg tggtcggcag cggcgccttc ctcgccaaca
     4321 gtttcgcggg caatggcggc aacgtggatc tcggcgtgaa catggtgaac tggctggcca
     4381 atgaggatag cctcatcacc ctgcaaccgc gtgccgcgaa agacagcaac ctgacgctca
     4441 accggacgca gctcaacgtc atcagcttcg gcttcctgat aggcctgccg ctgctgctgg
     4501 cgggggttgg cgtcaggatg tggtggaaac gccgccgcgc ataaacttaa aaaactttca
     4561 tccacgaaac acacgaaaag cactaaaaaa atatctaatc gaaagtcaca acttgcccag
     4621 caggtgacgt atgtgctcat gaaatgcttt ttaatttgtt cgtgtttttc gtggacaact
     4681 gctttttcga gaataaatta aggcatgcct gaactccccg aagtcgagac cacccgccga
     4741 ggactggagc cgcatctggt cggcgcgacc gtgacggatg tggtggtccg caatgcgcga
     4801 ctacgctggc cgatcccgga tgaactgccg ttgcgcggac gcgccattcg cgcactgcac
     4861 cgccgcgcaa aatatctgct ggtcgaattc gagcacggca ccctgatcct gcatctgggc
     4921 atgtccggca gcctgcgcat ccagcccgtc ggcacacccg cagagaaaca cgatcacttc
     4981 gacctggtgc tcgccaacgg cacgctgatg cgcctgcgcg acccgcgccg cttcggcgcg
     5041 gtgctatggc atgaaggcga catccacagc catccactgc tcgccgcact cgggcccgaa
     5101 ccgctggagc agggcccaca gcaatccgcc ttcgacgccg cacatctcta tcgcgccacg
     5161 cgcgaccgca gcgtcagtat caaacaatgc atcatggaca atcacatcgt ggtcggcgtc
     5221 ggcaacatct acgccaacga ggcgctgttc cgcgcaggca tcaaaccgcg actcgcggcg
     5281 ggtaaattaa gccgcgagcg ttgcgccaga ctggtcgaag cgatacgcgc cacgctgacc
     5341 gaggccatcg cgcagggagg cagcacgctg cgcgatttcg tgaacgcatc cggccaaccc
     5401 ggctacttcc agcagcatta ctgggtctat ggccgcgcag gcgaatcctg ccgtcgctgc
     5461 ggcacggtca tcagacaaat caagcaaggg caacgctcca gcttctactg cgccacatgc
     5521 cagcggtaac tgcttgacct ttcaatcgct tgctccaaca atgcccgcag accaaccgac
     5581 acgatcagcc cacgtgcaca ccatgaacgc aaaacaggaa catctggaac tgggacaatt
     5641 catcccgccc gtctaccact acaacatgtt gaccaatccg gaacgcatgg ctgctttccg
     5701 cacggccatc gaactggcgg ttccggtcgg cgccaaggtg ctggaactgg gcggcggaac
     5761 ggcagtgctg tcgttcttcg cagcccagcg cgcgtcaaaa gtgtggtgcg tagagaagaa
     5821 tcctgaaatg gtgcgcgagg cacgccgcat tctggcgctg aatcccggca gcgaacgcgt
     5881 cgagctggtc gaagccgacg cctacgacta tctgccgccc gagccggtgg atgtggtgat
     5941 ctgcgagatg ctgcatgtcg gcatgctgcg cgaaaaacag atccccgtga tcgaatcgtt
     6001 caaacgcaac tatctggcga agttcggcgc gccgctgccg cgtttcatac cggaggcatt
     6061 cattcaggcg gtgcagccgc tgcaacaatc gttcgatttc gacggctact acgcccccag
     6121 caccttcttc cagaatccgt ccgagatcca gccccgaagc gaagaactgg gcaaaccggc
     6181 gatctaccag atggccatct acgaggcgcc cttgccggcg gattgcaact ggcggggcga
     6241 gatcgtcatc gaacaggatg gcacgctgaa cgcactgcgc ttcatcacca agaacctgat
     6301 cgccatcgat gaggccgcgc agcgcaccgt agactggttc ggccattatc tggtgacgcc
     6361 cctgtccgac ccgctgtccg tcacagcggg ccaacgactg gcggtatgct tcgattaccg
     6421 tccgggcgac ccgatctcgg cactgcgccc cagcgtcacc ccgataacct gagcaccgaa
     6481 tagcctgaaa caggtataat gcgcccgccc tgcacccagc caatcggtgc agcggccttt
     6541 ccagttcact ccagatcgtt tcgatccctt attcgtctgc cccgattggt gccgttcaac
     6601 gcgaacggca ggccgtcgca ggagttttgt tttgtcagaa aaaattagtt tgtccaatga
     6661 tgtcactttc gccggcctga agctggcaga acccatattg cgcgccatcg ccgacgccgg
     6721 ttacacgaac cctacgccgg ttcaggcaca ggcgattccg caggtactgg caggcggcga
     6781 tctgctggcc ggcgcacaaa ccggcaccgg caagaccgca ggtttcaccc tgccggtcct
     6841 gcatcgcctg tccgagaagc ccgccgcgaa tccccgcgca ggcgctccgc gctgcctgat
     6901 cctgaccccg acgcgcgaac tcgccgcgca ggtggaagaa tcggtgcaga cctacggcaa
     6961 atatctgccg ctgaaatcca tggtgatgtt cggcggtgtg aacatcaatc cgcagatcaa
     7021 ggcattgaag agctcggtcg acattctggt cgccacgccc ggacgcctgc tcgaccacgt
     7081 cggacagaag acgctggatc tgtccagcgt cgagatcctg atcctcgacg aggctgaccg
     7141 catgctggac atgggcttca tccgcgacat caagaaaatc ctcgcgctgc tgccgaagca
     7201 acgccagaac ctgctgttct cggccacctt ctcggacgag atcaagaccc tggccgacgg
     7261 cctgctgcac aatccgggct atgtcgaagt ggcgcgccgc aacaccacct ccgaactggt
     7321 cgagcagagc gtgcacctga tcccgcagaa gctgaagagc tatctgctcg cgcacctgat
     7381 caagcacaac gactggaagc aggtgctggt gttca
//
LOCUS       DASUIV010000200         3389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_698759, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000200 DASUIV010000000
VERSION     DASUIV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698759"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..554
                     /locus_tag="VFW59_07330"
     CDS             390..554
                     /locus_tag="VFW59_07330"
                     /inference="COORDINATES: protein motif:HMM:NF015798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX5364065.1"
                     /translation="MSRGSEHSKAICTLCADICQSCGDECAKHQMAHCQQCAKACLKC
                     AEECRKMVAL"
     gene            551..754
                     /locus_tag="VFW59_07335"
     CDS             551..754
                     /locus_tag="VFW59_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364066.1"
                     /translation="MKTLLAFAVGAVALLTTGVTLAQNGNMMNGGSWGGGWMGGYGGV
                     WTPILLVIVVVALVAWILGRGGK"
     gene            831..1256
                     /locus_tag="VFW59_07340"
     CDS             831..1256
                     /locus_tag="VFW59_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005180709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364067.1"
                     /translation="MTHFRFIPLAAVLASATLLAACNTAPAMPMGAASANSMATPDQM
                     ARMDAQMKTMQGMHEKMMNAKTPAERSKMMAEHRKAMQDGMEMMSSMSSAGMGDMKGM
                     QGMSGDMGAHHQMMAKRMEMMQTMMKMMMDQMPPAPGKQ"
     gene            1267..1731
                     /locus_tag="VFW59_07345"
     CDS             1267..1731
                     /locus_tag="VFW59_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX5364068.1"
                     /translation="MNPRTESPAGPPSGAAYGFYCTLGDTSFADAVARVTEALKVEGF
                     GVLTDIDVQATMKAKLGVDVPPYRILGACNPPLAHRALTAQPDIGLLLPCNVIVREVA
                     ADSFTVGFMDPVAVMRLTDDPEVAKIADEVRGRLERVRTALLTAAPGSTPSH"
     gene            1862..>3389
                     /locus_tag="VFW59_07350"
     CDS             1862..>3389
                     /locus_tag="VFW59_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5364069.1"
                     /translation="MAVLIDPQRTKVLRATPRLMQILRVLARHKFLGALFGRGHWPSP
                     KEVRETFEALGLVFLKFGQVLALRRDLLPVAYINELELLHDQLPAMGIDVVRATVEVE
                     LGAPLTKLFSSFNETPLAAATIAQVHEATLMGGRHVAVKVQRPGLTAIISTDIAALTY
                     LVRLGEGIFPSLRALDLLVVVRELANSLHRETDFNREARSIVLIRAALADVPDLWIPD
                     VVAERSGATVLTMEYSAGERVDLYAKRHPEAMPRVVDTLVKLMLHSIFEEGLFHADPH
                     PGNVFVLPDGRLSLLDFGNTGDLDEPMRESLTLLLEAVVKGDARAATEAYLEMAPTSE
                     KVNRVGLLVDIKAVLYEIRRTNLADVSIGNAFESLLRAGSRNGVHNPAEFVQLTRAFA
                     ILESMIGILAPDYNHMGSFREEIARLTAQHFAPEQIKGTTNKLVRELERLIINAPGDT
                     RRILRRFAEGDLGRLPSLEALGGRFSRNLERLASSIVFAALVISGAMLMLMPLGGRHH
                     I"
BASE COUNT          729 a          932 c         1024 g          704 t
ORIGIN      
        1 ttccaaccag tgactggccg aataatccgg cagtaccgaa tcgaccgata catcgtggtg
       61 tggttggttt gcgcggcagt ttctgtgcga aacctggcat agatggcgtt gcaaaagatg
      121 acaggaagag ggcatgaaga cttacaacat gctgtggggg aatgcagaga agatgaggga
      181 cttttccgtt ttcatgtacc cacaatccag ggaaaattca aatgcacccc caaaaattca
      241 acgcttgcat cgaggcgtgt aatgcgtgcg cagtagcctg taatcactgt gccgcttcat
      301 gtcttcacga gcccaacgtc aagatgatgg cgcattgcat tgcattggac atggattgcg
      361 ctcaaatctg tgcactggct gcggcagcga tgtcgcgcgg cagtgaacat agcaaagcga
      421 tctgcacctt gtgcgccgat atttgtcaat cttgtggcga cgagtgtgca aaacaccaaa
      481 tggcgcattg tcagcaatgt gctaaagcct gcctaaagtg cgctgaagag tgccgcaaga
      541 tggtggcatt atgaagacct tgcttgcttt tgccgttggt gcggtagcac tgttgaccac
      601 cggcgtcacg ttggcgcaga acggaaacat gatgaacggt ggctcgtggg gcggtggctg
      661 gatgggtgga tacggcgggg tatggacgcc catcttgctt gttatcgtcg ttgtcgctct
      721 cgtcgcatgg atcctcgggc gaggcggcaa atgacagaag acaaacaccg cctctgaata
      781 aattctctct cgctattttc aacgttactt ccctcaccca ggagtctgcc atgacccatt
      841 tccgattcat tccacttgcc gctgtcctcg ccagcgctac cctattggcc gcctgcaaca
      901 ctgccccggc gatgcccatg ggcgctgcat ctgccaacag catggcgaca cccgaccaga
      961 tggccaggat ggatgcgcag atgaagacca tgcagggcat gcacgaaaag atgatgaacg
     1021 ccaagacccc ggcagaacgc agcaagatga tggccgaaca caggaaagca atgcaggacg
     1081 gcatggaaat gatgagcagc atgtccagtg ctggcatggg cgacatgaaa ggcatgcagg
     1141 gcatgtctgg cgatatgggc gcgcaccatc agatgatggc caagcgcatg gagatgatgc
     1201 agaccatgat gaaaatgatg atggaccaaa tgccgccagc gccgggcaaa cagtaaggag
     1261 atcgccatga acccacgcac tgaatccccc gcaggcccgc cgtcgggtgc agcctatggt
     1321 ttctactgca cgctgggcga caccagcttt gctgatgcgg tagcccgcgt gactgaagcc
     1381 ttgaaggttg aaggctttgg cgtgctgact gacatcgacg tgcaggcgac gatgaaggcc
     1441 aagctggggg tcgatgttcc cccctatcgc atcctcggcg catgcaaccc tccgctggcg
     1501 caccgcgcct tgactgctca gcccgacatt ggcctgctgc tgccctgcaa tgtcatcgta
     1561 cgggaagtgg ccgctgatag cttcacggtg ggtttcatgg accccgtggc tgtgatgcgg
     1621 ctcaccgatg atcctgaggt agccaagatt gcggacgagg tccgcggccg cctggagcgg
     1681 gtgcgtacgg cgctgctaac ggcagcgccc ggcagcaccc cgagccatta agtgtgcgcg
     1741 ccgatgccga ctattgggtg atctcttgaa tacggagcga cttcacactt tccggtaacg
     1801 gcatcaagcg ccaagattgg ttttttagaa gccaatctga aactttttaa aggtaaagcg
     1861 catggctgta ttgatcgacc cgcagaggac caaagtgctg cgagcgacgc cgcgactaat
     1921 gcaaattctg cgcgtgctcg ctcgacacaa gttcctggga gccttgttcg gcagggggca
     1981 ttggccgtcg ccgaaagagg ttcgggaaac cttcgaagcg ttggggctag tctttcttaa
     2041 attcggacaa gtgctcgcct tgcggcgcga cctgctgccg gtcgcctaca tcaatgagtt
     2101 ggaactgctg cacgaccagt tgccggcgat gggcattgac gtggtgcgcg cgacggttga
     2161 agtcgaactg ggcgccccgt taactaagtt gttctcgtcg ttcaacgaga cgccgctagc
     2221 cgccgccacc atcgcccagg tgcatgaggc gacattgatg ggcgggcgtc acgtggccgt
     2281 gaaggtgcag cggcccggcc ttaccgcaat aatctccacg gatatcgcgg cactgactta
     2341 tctggtacgg ctgggagaag ggatttttcc gagtctacgc gccctcgacc tcctcgttgt
     2401 agttcgtgaa ttggccaata gtcttcaccg ggaaactgat ttcaatcgcg aagcgcgttc
     2461 cattgtgctc attcgcgccg cgctggcaga tgtccccgac ctctggattc cggatgtcgt
     2521 ggcggagcgt tcgggagcaa ccgtgctcac gatggaatac tcggccggcg aacgggtgga
     2581 tctgtacgcc aagcggcacc cggaagcgat gccgcgggtg gtcgacacgc tggtaaagct
     2641 gatgctgcat tcgatctttg aagaagggtt gtttcatgcc gacccgcatc caggcaatgt
     2701 ctttgttctg ccggacgggc ggctctccct tctggatttc ggcaatacgg gcgaccttga
     2761 cgagccgatg cgcgaatcgc tgacgcttct gctcgaagcg gtcgtcaaag gcgacgcccg
     2821 ggcagccacc gaggcctatc tcgaaatggc accgacgagc gaaaaagtca accgtgtggg
     2881 gttgttggtg gacataaagg cagtgcttta tgaaatccgc aggaccaatc tggcggatgt
     2941 ttcaatcggg aatgcgtttg aatcgctact gcgtgccgga agccggaacg gggtacacaa
     3001 tcccgccgaa ttcgtccagc taacgcgtgc tttcgccatc ctggagtcga tgattggcat
     3061 actcgccccg gactacaacc acatgggatc gtttcgcgaa gagatcgcac gactgaccgc
     3121 tcaacacttt gcaccggagc agataaaggg aacaacaaac aagcttgtgc gcgaactgga
     3181 gcgcctgata atcaatgcgc ccggcgatac gcgacgcatc ctgcggcgtt tcgccgaagg
     3241 cgatctgggc cgactgccga gcctggaggc gctgggaggt cgctttagcc gcaatctgga
     3301 gcggctcgcc agttccattg tcttcgcggc gctcgtgatc agcggcgcga tgctgatgct
     3361 tatgccgttg ggcggcaggc atcacattc
//
LOCUS       DASUIV010000201         3995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_790819, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000201 DASUIV010000000
VERSION     DASUIV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790819"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFW59_07355"
     CDS             <1..247
                     /locus_tag="VFW59_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5364070.1"
                     /translation="GIRINAINPGPIDTGGFQRLPLPPDVHQAIKTDIASRSPIKRFG
                     TPEEVAKVALFLASDDSAYVVGEEIVVDGGISLVCLP"
     gene            256..1056
                     /locus_tag="VFW59_07360"
     CDS             256..1056
                     /locus_tag="VFW59_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364071.1"
                     /translation="MSNKEQQDAIRTRIWQEEPEPDNPFATRTARCRGYNVYGEMLGQ
                     ARWVEMLFLLFHEELPMPAQADLLEALAVALANPGPRDASVHAAMCGGICGSTAASSL
                     MAALAVGAGQLAGGREVFLAMQGWAECGTDLDVWQRHLGTLDATPGSIWPKPEHPPGF
                     DPHGVSTATSVTQTLACLTQYSAGPCLPWLQQNLTELETAAGYPLALSGVAAAAFADL
                     GCTPEQGEMLHLLLRLPGAAAHALEQRTVGYKKFPFGVVELEKTGEQT"
     gene            1053..1874
                     /locus_tag="VFW59_07365"
     CDS             1053..1874
                     /locus_tag="VFW59_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364072.1"
                     /translation="MTRPDLLKEYTGILKSRMGAAFPGSHAIFRGHDLHADLKDLDWV
                     ELYVFGITGRRFTKAQLRLMQAIWTYTSYPDVRLWNNRVAALAGSSRSTGTLGMAAAL
                     AVSEAAIYGGGILARAIDFFVRTQKQLENGGELSACIREELTTHRNLGGYGRPLVNGD
                     ERIPHLMALAKSLGLDQGPYLRLAFVIEDALLAGRWRMKMNYAALVAALGADLGLSAR
                     EFSLFMFPVFLAGMPSGYIEAVERPEGTLYPVSCGGIRYEGQSPRAWPGISGERE"
     gene            1927..>3995
                     /locus_tag="VFW59_07370"
     CDS             1927..>3995
                     /locus_tag="VFW59_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5364073.1"
                     /translation="MEALNYRTLYVLLALAWSGTAAAQLSEEEELAQVYGDKATISLA
                     TGSRQPVARAPAVATAITSQDIAAMGATDLDQALASVPGLHVSVTNIAMNPIYSFRGI
                     HTRYNPQVLMLVNGLPITNAFAGDRSQLWGGMPLENVARIEIIRGPGSALYGADAFSG
                     VINVITKTAADIRGTEYGLRAGSFNSKDAWIQHGGQLGALDAAFYLGAGNTNGSKGVI
                     RQDAQSAWDACFSAGCPTAAPPIAAIPGYTPVSRAPGPLNTERKALDARADLSFEAWR
                     FRAAYQQRELGVGAGLAGALDPNARAPESRTYLDLSYEQPNWVTNWDISAVVGFFDIK
                     EKPANPYFTLFPAGAFGGAFPNGMIANPAHSERQTHGSVSAVYNGFEQHRVRVGTGYR
                     AVDLYQTSELKNFDARFVPLPALVQSAGNPALVYLLPHKRNLAFAFAQDEWSFARDWT
                     LTAGVRHDSYSDFGGTTNPRVALVWDAAYNIVVKAMHGTAFRAPSFSEQYAINNPVTV
                     GSPNIKPETIATDELAFAWQATGDLQANLNFFHYRMRNIIRPVGTTYNNSGDQTGRGL
                     ELEAIWSATDDLKLSGSYSLQHSIDSASGKDAGMSPHRRLFARADWQVAPFWQLGTTL
                     NHVAGRMREPGDPRALIPDYTLVNLTMRREKFAGNWEVRAMVSNLFDRKAWEPTFLTV
                     GMPSDLPL"
BASE COUNT          797 a         1245 c         1200 g          753 t
ORIGIN      
        1 cggcatccgc atcaatgcga tcaatcccgg ccccatcgac accggcggct tccagcgcct
       61 gccgctcccg ccggatgtgc atcaagccat caaaaccgac attgcaagca gatcgccgat
      121 caagcgtttc ggcacgcccg aagaagtcgc aaaggtcgcg ctctttctcg cctccgacga
      181 ctcggcttat gtcgtcggcg aggaaatcgt cgtagacggc ggcatcagtc tggtttgttt
      241 accttaggag caggcatgtc caacaaggaa caacaggatg ccatccgcac ccgcatctgg
      301 caggaagagc ccgagccaga caatccgttc gcgacccgca ccgcccggtg tcgcggttac
      361 aacgtctacg gcgaaatgct cggacaggcg cgctgggtcg aaatgctgtt cctgctgttc
      421 catgaggagt tgccgatgcc agcgcaagcg gatctgctgg aagcattggc agtcgcactg
      481 gccaaccccg gtccgcgcga tgcgtcggta catgccgcga tgtgcggcgg catctgcggc
      541 tcgaccgcag catcctcgct gatggccgcg ctggctgtcg gggccggaca actggccgga
      601 gggcgcgagg tatttctggc gatgcaaggc tgggcagaat gcggcaccga tctcgatgtc
      661 tggcagcgac acctcggtac tctggacgcg actcccggca gcatctggcc gaagccggaa
      721 catccgcccg gtttcgatcc tcatggcgtc agtacggcca cttcggtgac gcagacgttg
      781 gcctgtctga cgcaatacag cgctggtccc tgtctgccgt ggctacagca gaacctgacc
      841 gaactcgaaa cggcggcggg ctatccgctc gcgctgtccg gcgtggctgc cgccgcgttc
      901 gccgacctgg gctgcacgcc ggaacaaggc gagatgttgc atctgttgtt gcggctgccc
      961 ggcgctgcgg cccatgcgct ggaacaacgc actgtcggct acaaaaaatt cccgttcggc
     1021 gttgtagaac tggaaaaaac cggagagcaa acatgacccg ccccgacctg ctgaaagaat
     1081 ataccggcat attgaaaagc cgcatgggcg cggcctttcc cggcagccat gcgatcttcc
     1141 gtggccatga tctgcatgcc gacctgaagg acttggattg ggttgaactg tatgtgttcg
     1201 gcatcaccgg acgacgcttc accaaagcgc aattgcggct gatgcaggcc atctggactt
     1261 acaccagcta ccccgatgtc aggctgtgga ataaccgtgt cgccgcgctg gcgggcagct
     1321 cgcgcagcac cggaaccttg ggtatggcgg cagccttggc ggtttcagag gcggctattt
     1381 atggcggcgg cattcttgcc cgtgccatag attttttcgt ccgtacgcaa aaacaactgg
     1441 aaaacggcgg ggaattgagc gcttgcatcc gggaggaact gacgacgcat cgcaacttgg
     1501 gcggctatgg acgtccgctg gttaacggag atgaacgcat accgcactta atggcgcttg
     1561 ctaaaagcct gggcctggat caaggcccat atttgcgcct cgcttttgtg atcgaagacg
     1621 cactgcttgc cggacgctgg cgcatgaaaa tgaattacgc cgcattagtc gctgcgctgg
     1681 gtgccgatct cggactgtcg gcacgcgagt tttccctgtt catgtttccg gtgttcctgg
     1741 ccggtatgcc gtcgggatac atcgaggccg tggagcgccc ggaaggaacg ttatatccgg
     1801 tttcttgtgg cggcatccgg tatgagggac aatccccgcg agcctggccg gggataagcg
     1861 gggaacgaga atagcagcac atcgaaattt gcatcattga cccaaacgaa catcaggggt
     1921 atcgcaatgg aagctttgaa ctatcgcaca ctctacgtct tgctggcttt ggcctggagc
     1981 ggcactgctg cggcgcagct ttccgaggag gaagagctgg cgcaggtcta cggcgacaag
     2041 gccacgatca gcctcgccac cggcagccgc caaccggtgg ctcgcgctcc cgctgtagcg
     2101 acggccatta cctcgcagga catcgcggcg atgggagcaa ccgatctcga tcaggcgctg
     2161 gccagcgtgc caggcctgca cgtttcggta acgaatatcg cgatgaatcc gatctacagt
     2221 tttcgtggca tccatactcg ctacaacccg caggtgctga tgctggtcaa cggcctcccg
     2281 atcaccaacg cttttgcggg cgatcgcagc cagctctggg ggggcatgcc gctggagaac
     2341 gtcgcgcgca tcgaaatcat tcgcgggccg ggctcggcgc tgtacggcgc ggatgctttc
     2401 tccggcgtga tcaatgtcat caccaagacc gccgccgaca ttcgcggcac cgagtatggc
     2461 ctgcgtgccg gctcgttcaa ctcaaaggat gcgtggatac agcacggcgg ccagctgggc
     2521 gcactggatg cggccttcta tctgggtgca ggcaacacca atggttcgaa aggcgtcatc
     2581 cggcaggatg cgcaatccgc ctgggatgcg tgcttcagcg cgggatgccc aacagcagcc
     2641 ccgcccatag cagccatacc cggttacacg cccgtatcgc gcgcccccgg accgctcaac
     2701 accgaacgca aggcgctcga tgcacgagcc gatctgtcct ttgaagcatg gcgtttccgc
     2761 gcagcctatc agcaacgcga actgggcgta ggggcagggc ttgccggagc gctggatccc
     2821 aatgcgcgcg cgcccgaatc cagaacctat ctggacctga gttatgagca acccaactgg
     2881 gtgacaaact gggatatatc cgctgtcgtt ggatttttcg acatcaagga aaaacccgcc
     2941 aacccatact tcacgctctt tccggctgga gcattcggcg gagcctttcc caacggcatg
     3001 atcgccaatc ccgcccactc ggagcgccag acgcatggca gcgtttccgc cgtctataac
     3061 ggcttcgagc agcatcgcgt gcgcgtgggc acagggtacc gggcagtgga tttgtaccag
     3121 accagcgaac tgaaaaactt cgatgcgcgc tttgttccgc tgcccgcact ggtgcagtca
     3181 gccggcaatc ccgcgctggt ctacttgctg ccgcacaaac gcaacctggc ttttgcgttc
     3241 gcgcaggacg aatggagctt cgcccgcgac tggactctga ccgcaggcgt gcggcatgac
     3301 agttattccg atttcggcgg caccaccaac ccgcgcgtcg cgctggtgtg ggatgccgcc
     3361 tacaacatcg tggtcaaggc gatgcacggc acggcttttc gcgccccatc gttctccgaa
     3421 caatacgcca tcaacaatcc ggtgacggtc ggcagcccga acatcaagcc ggagacgatt
     3481 gccaccgacg agctcgcgtt cgcctggcaa gccaccggcg atctgcaagc caacctcaat
     3541 ttcttccatt accgcatgcg caacatcatc cgaccggtcg gcacgacgta caacaattcc
     3601 ggggatcaaa ccggacgagg attggagctg gaggcgatct ggagtgccac cgacgacctg
     3661 aaactttccg gaagctattc gctgcaacac tccatcgata gcgcctccgg caaggatgcg
     3721 gggatgtcgc cgcatcgtcg gctgttcgcg cgcgccgact ggcaagttgc gccattctgg
     3781 caattgggaa ccacgctcaa tcatgtggcc ggacgaatgc gcgaacccgg cgatccccgc
     3841 gcgctcattc ccgactacac cttagtgaac ctgaccatgc ggcgtgaaaa gtttgccggg
     3901 aactgggagg tgcgcgcgat ggtgagcaac ctgttcgacc gcaaagcctg ggagccaact
     3961 ttcctgacgg tgggaatgcc ttccgatctg ccgct
//
LOCUS       DASUIV010000202         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1071243, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000202 DASUIV010000000
VERSION     DASUIV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071243"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..888
                     /locus_tag="VFW59_07375"
     CDS             112..888
                     /locus_tag="VFW59_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5364074.1"
                     /translation="MTIRAMWKGVIQFNAIRVPVKLYGAIEDRNVHFRLLHRGDLEPV
                     HQALVNPQTDEVVSRENTGRAYITESGDRVLLNQSELEALEPEPSRDIEILSFLPQHV
                     IDHRWYDRPYYLGPDEDSAEFFALADALEAMQVEGLARWVMRNKSYVGALQLYQGYPM
                     LMSLHHTEEVIPVEALAPPKGPPLEQRELGMARQLIEMLESEFEPQEYHDEFRDRVLA
                     MLDAKAHGKQVHKRALPQRESSIDLSQALESSIQQMRKRA"
     gene            917..1807
                     /locus_tag="VFW59_07380"
     CDS             917..1807
                     /locus_tag="VFW59_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5364075.1"
                     /translation="MDKDDTDQVEEQEDEQEQPHAIWSGIIAFGLVSLPVSLFPAHSG
                     KIALKMVDAKGALLKRQFFCADDNRPLERDDIVRSYEIEKYHYVVVEDEELDALEPKK
                     SREIDLKRFVPVSAINPVYFEHAYFLVPDGDTTKAYRLLAKSMEDEHKAGIATFVMRG
                     KDYLVAIIAEGGILRAETLRFHDELRTPEQIGLPQRQPADKNRVERMRTAIKKMTHKH
                     FDSELLSDPHTRNLQQRIEAKLKAGTDVLKAPEPAAAEAESNVIDLMQVLKERLQGLR
                     PHASDQPAKPERSAHREKSR"
     gene            1944..2459
                     /locus_tag="VFW59_07385"
     CDS             1944..2459
                     /locus_tag="VFW59_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5364076.1"
                     /translation="MNIRNLWSFPMLAVSLALCLPQLAHAEMSATEPAARNIEEMKFG
                     PIAPLPTCMSGAVQNGDPAKGAFIVAVKLTTGCSIPWHWHSAAEYLMIVSGTGRMEMK
                     DNQPKTIRAGSFVQLPAHHVHQFTCKNKCTFFFYSDGAWDIHYVDAQGTEIPPDDALK
                     AVKEKPAQAMK"
     gene            2651..3655
                     /locus_tag="VFW59_07390"
     CDS             2651..3655
                     /locus_tag="VFW59_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5364077.1"
                     /translation="MRISLLRQRLRDLGAKPCHADRLLRGWSQVSSYDRKGSPAEDFF
                     PLAMRNELPAIEAELNGLAHLHGEYPADDEVARLLVKLSDGQMVESVLLPRGGLCVST
                     QVGCAVGCVFCMSGRAGLLRQLSSAEIVAQVVLARRRRAVSKVVFMGMGEPAHNLDNV
                     LEAIELLGTQGNIGHKNLVLSTVGDLRVFERLPLGTVKPALALSLHTTDAALRAKLLP
                     HAPQIPVEELVARAEAYARATNYPVQYQWTLIEGINDSDAEVERIVELISGKYAVMNF
                     IPFNEVDGLAYRRPSPERIAAMVQRLKQHGILAKIRDSAGQEIEGACGQLRARAVKAR
                     "
     gene            3758..4606
                     /gene="asd"
                     /locus_tag="VFW59_07395"
     CDS             3758..4606
                     /gene="asd"
                     /locus_tag="VFW59_07395"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441436.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEX5364078.1"
                     /translation="MSDRLAVLPQYLIPKQLLTVLAGKFANARLGGLTTAVIHRFVAR
                     YGVNMQEAANPDIAGYASFNEFFTRPLREGARPQAEADLICPVDGAISQCGAIERDSI
                     FQAKGHSYTTTALVGGDADLAAQFADGSFATLYLSPKDYHRIHMPCAGRLRRMIHVPG
                     ELFSVNPTTARGVPGLFARNERVVCVFDSDFGPFVLTLVGATIVGSMATVWHGVVNPP
                     RPGTVREWRYDGQEITLQKGQEMGRFLLGSTVVMLFPKDALNFNPEWQPAKALRMGEA
                     MGNKPR"
     gene            complement(5079..>5438)
                     /locus_tag="VFW59_07400"
     CDS             complement(5079..>5438)
                     /locus_tag="VFW59_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5364079.1"
                     /translation="TLILFQQPFGINALVGLIALSGILMRNTLILIGQINHNKAAGLD
                     PFRAVVEATVQRARPVILTALAAILAFIPLTHSVFWGTLAYTLIGGTFAGTILTLVFL
                     PAMYSIWFKIKPVQSQS"
BASE COUNT         1182 a         1580 c         1632 g         1044 t
ORIGIN      
        1 ttatggctcg gcaggctggg ctgttctctt gaaattattc cggacagcag tgggccttcg
       61 ccggaatgac gagttgcgga attgcggtcc ttaacatgat ggaggtgcaa gatgactatc
      121 cgcgcgatgt ggaaaggtgt gatccagttc aatgcaatcc gcgtgccggt gaagctgtac
      181 ggtgccatcg aggatcgcaa cgtgcacttc cggttgctgc atcgtggcga ccttgagccg
      241 gtccatcagg ccctggtcaa tccgcagacc gacgaggtcg tttcccgcga aaacaccggc
      301 cgcgcctaca tcaccgaatc gggcgaccgg gtgctgctga atcagtcgga gctggaggcg
      361 ctggagccgg agccgtcgcg cgacatcgag attttgagct tcctgcccca gcatgtgatc
      421 gatcatcgct ggtacgaccg tccctattac cttgggccgg acgaagacag cgcggaattc
      481 ttcgcactgg ccgatgcgct ggaggcgatg caggtggaag gtctcgcgcg ctgggtgatg
      541 cgcaacaagt cctatgtcgg cgcgttgcag ttgtatcagg gctatccgat gctgatgtcg
      601 ctgcaccaca ccgaagaggt catccccgtg gaagcgctcg ctccgcccaa ggggccgccg
      661 ctggaacagc gcgagctggg catggcaagg cagttgatcg agatgctgga atcggaattt
      721 gagccgcagg aatatcacga cgaatttcgc gaccgcgtgc tggcgatgct ggatgccaag
      781 gctcatggaa aacaggtgca caaacgcgct ttgccacaac gcgaatcgag cattgatcta
      841 agtcaggcgc tggagagcag tatccagcag atgcgcaaac gtgcctgagg tgtgacacga
      901 ggaaaggtgg tgtgacatgg acaaagacga cactgatcaa gtcgaggaac aggaagatga
      961 gcaggagcag ccccacgcca tctggtcggg gatcattgcc ttcggtctgg tgagtcttcc
     1021 ggtcagcctg tttcccgccc acagcgggaa gatcgcgctg aagatggtcg atgccaaggg
     1081 tgcgctgctc aagcggcagt tcttctgcgc cgacgacaac cggccgctgg agcgggacga
     1141 tatcgtgcgc agttacgaga tcgagaagta ccactatgtg gtggtcgaag acgaagagct
     1201 ggatgcgctg gagccgaaga aatcgcgcga gatcgacctg aagcgtttcg tgcccgtcag
     1261 cgctatcaac ccggtctatt tcgaacatgc ctatttcctc gtccccgacg gcgacacgac
     1321 caaggcctac cggctgctgg caaagagcat ggaggacgag cacaaggccg gcatcgccac
     1381 cttcgtgatg cggggcaagg attacctggt ggcgatcatc gcagagggag gcatcctgcg
     1441 tgcggagacg ctgcgctttc acgacgagct gcgcacgccc gagcagatcg gcctcccgca
     1501 acgccaaccc gccgataaaa atcgcgtcga gcggatgcgc acagcgatca aaaagatgac
     1561 gcataaacat ttcgacagcg aactgctgag cgacccgcac accagaaatc tccagcagcg
     1621 catcgaagca aaactcaagg caggcaccga cgtgctgaag gccccggaac ccgcagcggc
     1681 cgaggccgaa agcaacgtga tcgacctgat gcaggtgctc aaggagcgct tgcagggact
     1741 ccggccgcat gcatctgacc agccggccaa accggagcgt tccgcgcatc gggagaaatc
     1801 gcgctagcac catcatcggg agcgcacgga tacacgatgt tgcgtattga gatgttggcc
     1861 ggttcaacta tatttcaatg acagcgattg cgcgtccatg cgcatccccc tctcaaccgt
     1921 atctgctcat acaggaggca aaaatgaata tcagaaacct atggtcgttc cctatgctgg
     1981 ctgtgtcctt ggccctgtgt ctgccgcagc ttgctcatgc tgagatgtcc gcaaccgaac
     2041 ctgcggctcg taacatcgaa gagatgaagt tcggtccgat cgccccgctg ccgacctgca
     2101 tgtcaggggc cgtgcaaaat ggcgatccgg cgaaaggcgc tttcatcgtc gccgtcaaat
     2161 tgactacagg gtgttcgatc ccctggcatt ggcatagtgc ggccgagtat ctgatgatcg
     2221 tgagcggcac gggccggatg gagatgaagg acaaccagcc gaagacaatc cgggccggaa
     2281 gctttgtcca gctaccagct catcacgtcc accaattcac ctgcaagaac aagtgcacat
     2341 tcttcttcta ttccgacggg gcctgggaca ttcattatgt ggatgcgcag ggaaccgaga
     2401 tcccgcccga cgatgcgctg aaagccgtga aggaaaaacc ggcccaggcg atgaagtaga
     2461 ttccggtgcg gatgaaaaaa gggagtttgc gtgaccccgc gcacggggtc acgcgctgct
     2521 tgcgatccag ccctccccga ttctccccca ccgtcgtgaa ccgtccccca agtcgatgca
     2581 tgagttaaaa tccgtacagt ttccccgcac actttccaaa cccgctttga ttctcaccgc
     2641 ccactccccg atgcgcatct ccctccttcg tcaacgcctg cgcgacctcg gcgcgaagcc
     2701 ttgccacgca gatcgtctgt tgcgcggctg gagtcaggtg agttcgtacg acagaaaagg
     2761 cagtcccgca gaggattttt ttccgctggc aatgcgcaac gagttgcccg ccatcgaggc
     2821 ggagctgaac ggactggcgc acctgcacgg tgagtatccc gccgacgatg aagttgcccg
     2881 gctgctggtc aagctgtctg acggccagat ggtggagagc gtgctgctgc cgcgcggtgg
     2941 tctgtgcgta tcgacgcaag tcggttgtgc ggtgggatgt gtgttctgca tgagcggccg
     3001 agccggtttg ctgcgccagc tcagcagcgc cgagatcgtc gcgcaggtgg tgctcgcgcg
     3061 ccgacgccgc gcggtgagca aggtggtgtt catgggcatg ggcgaaccgg cccacaatct
     3121 cgataacgtg ctcgaagcaa tcgagttgct tggcactcag ggcaacatcg gccacaagaa
     3181 tctggtgctc tcgacggtcg gcgacctgcg cgtgttcgag cggcttccgc tgggaacagt
     3241 caaacccgcg ctcgcgcttt cgctgcacac caccgatgcg gcgctgcgcg ccaaattgct
     3301 gccgcatgcg ccgcagattc ccgtagaaga actagtggcg cgcgccgaag cgtatgcgcg
     3361 cgccaccaac tatccggtgc aataccaatg gacgctgatc gagggcatca acgacagcga
     3421 tgccgaggta gagcgaatcg tggagctcat ctccggcaaa tacgccgtga tgaacttcat
     3481 ccccttcaac gaagtcgatg gcctggccta tcgccgccca tcacccgaac ggattgcagc
     3541 catggtgcag cgcctcaaac agcacggcat cctcgccaag atacgcgatt cggcaggaca
     3601 ggagatcgag ggggcgtgcg ggcagttgcg ggcgcgggca gtgaaagcga gataagccac
     3661 accggccttc gccggtatga cgacctgatg aattattcgg gcttaattcc atctcgcccc
     3721 gttataattc gccctttcgt cgttactgaa agtcatcgtg tctgatcgtt tggccgtgtt
     3781 accgcaatac ctgatcccca aacagttgct gactgtgctg gcaggcaagt ttgccaatgc
     3841 ccgtctgggt ggcttgacca ccgccgtaat ccaccgcttt gtggcgcgtt acggcgtgaa
     3901 catgcaggag gcggccaatc cggacatcgc cggttatgcg agcttcaatg agtttttcac
     3961 gcggccgttg cgcgagggcg cgcgcccgca ggcggaggcc gatctgatct gtccggtgga
     4021 cggcgcgatc agccagtgcg gcgccatcga acgcgacagt atttttcagg ccaaggggca
     4081 cagctatacg accacggcgc tggtcggcgg tgatgccgat ctggccgcgc aatttgccga
     4141 cggcagcttt gccacgctgt atctcagccc caaggattat caccgtatcc acatgccgtg
     4201 cgcgggacgc ttgcgccgca tgatccatgt gccgggcgag ctgttttcgg tgaacccgac
     4261 gactgcgcgc ggcgtgccgg gtctgttcgc acgcaatgag cgggtggtgt gcgtgttcga
     4321 tagcgacttc gggcctttcg tattgacgct ggtcggcgcc actatcgtcg gcagcatggc
     4381 aacggtgtgg catggcgtgg tcaacccgcc ccgccccggc acagtgcgcg agtggcgcta
     4441 cgatggtcag gaaatcaccc tgcagaaggg ccaggagatg ggccgcttcc tgctcggctc
     4501 cacagtcgtg atgctgttcc ccaaagatgc gctgaacttc aatcccgaat ggcagcctgc
     4561 caaggcgctg cgcatgggcg aagcgatggg caacaagccg cgctaaggct ctggcgacta
     4621 aaggaaagta ggcatccaca aaaccggttt attggagtag catgattttt ccttaatcac
     4681 gacaaggagt tcgatcatgc ccaagtcatc acatcacgca aatcgggata aagacatgaa
     4741 aaaggagcct ctgatgacgc cgaaggaaaa aaggatggcc aaacgtgcga agaagcatag
     4801 ccaggatgtt caaccgctta tcgcacctcc tgataccacc gctcattaag cgcccgtctg
     4861 tcgcctgaca atctctaatg cagaaacaga aagtccggca tcgccgggct ttctgttttt
     4921 ataactgtct attctttatc gaatccggtc gccctgcttc aaatacgctg tgctgcttat
     4981 atgcagccga cagaagacaa gattcaatcc tcgcggtgga ctttgtctgc cctaaccaca
     5041 atctgcatga aggataaatg atggatcccg attgcagatc agctttgcga ttggactggc
     5101 ttgatcttga accagatgga atacatggcc ggcaaaaaca ccagggtcaa aatcgtcccg
     5161 gcaaaggtgc cgccaatcag tgtgtaagcg agcgtccccc agaacaccga gtgagtcagc
     5221 ggaatgaagg ccaggattgc ggccagtgca gtcaagatca ccggtcgcgc acgctgcacg
     5281 gttgcttcaa cgaccgcacg gaacggatcc agccccgccg ccttgttgtg gttgatctgc
     5341 ccgatcagga tcagcgtatt gcgcatcaga attccggaca gcgcaatcaa gccgaccagc
     5401 gcattgatcc cgaacggttg ctggaacaga atcagggt
//
LOCUS       DASUIV010000203         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_233671, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000203 DASUIV010000000
VERSION     DASUIV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233671"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..909)
                     /locus_tag="VFW59_07405"
     CDS             complement(4..909)
                     /locus_tag="VFW59_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5364080.1"
                     /translation="MAPALHRFSFFAMGSQCELQLYAGDESAASSAAQCAIDEVLRIE
                     ARYSRYRDDSILAKINRAAAQGTSLPIDEETATLLDYAYACHAKSDGLFDITSGVLRR
                     AWDFDASRLPEPRQLDALLPLVGLHKIRWERPRLSFDLPGMELDFGGIAKEYAADQAA
                     AVCVAQGIEHGLIELGGDIQVIGPHPDLSPWIVGIRHPRLADGIMTTVEISRGAIASS
                     GDYERYIVVDGVRYCHLLDPSTGWPVQGLASVSVISEQCLVAGSVATMAMLKGNAGKQ
                     WLAALGVPHLWMDEAGNSGGNIHPQ"
     gene            complement(920..2017)
                     /locus_tag="VFW59_07410"
     CDS             complement(920..2017)
                     /locus_tag="VFW59_07410"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX5364081.1"
                     /translation="MKPLNSLNFSGGPGALPESVLRQVQEAILEVPEVGLSILGISHR
                     SDWFAAVITELENNIRTLLGVSDNYHVLFLQGGATQQFSMVPMTLLRGKSHAAQYLHT
                     GYWSGKAIPEAKREGPIQVIWSGAECGYSRLPADAELAFSADAPYLHYISNETVEGLQ
                     FHRVLGRDDVPRVCDMSSDFLSHPVDAERFSLIYAHAQKNIGPAGVTVVLVRDELLKD
                     ANTDLPGFLNYRSHIQAHSNFNTPPVFSIYVVLLVSRWLLNEIGGVARMAEINHGKAE
                     LLYRMLDGSDGFYRGRAAVPDRSLMNVVFNLPSPELQQRFLTETQAAGFSGLAGHRAI
                     GGIRASIYNALTLSAVEQLAGFMEDFQRRAA"
     gene            complement(2043..3281)
                     /locus_tag="VFW59_07415"
     CDS             complement(2043..3281)
                     /locus_tag="VFW59_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364082.1"
                     /translation="MNDNDHMHPSETPFRGLAPFLRLSIAGADLQPVAQQLLALAQDN
                     GDDANLWMNLSLAMQCLGQRDLGMAMQQQALALKRIYHIAATIQPARFRLLMLMVPGD
                     LSANTPLECLLEDGDIDLDCYYVTPGDPLAQPLPEHDAVIVAIGESDDNHPTLEALEL
                     ALAHWPKPVINAPLYIRTTERAVASALLQDVPGLVIPPTLHASRPVLLEIAAGESSLP
                     ELSGGCDFPIILRPVGSQAGRDLDRITDPAGIALDLARVNAEDFFLSPFIDYSGTDGQ
                     FRKFRVALIDGVPYACHMGVSSHWMVHYVNAGMYEDAHKREEEAAFMANFDDFAQRHH
                     EALKAIHERTGLDYLCIDCAETQDGQLLIFEVDHVMVVHAMDPEELFPYKQFHMRKVR
                     DAFRDFLFRRAAEFAATKPS"
     gene            complement(3287..4183)
                     /gene="rsgA"
                     /locus_tag="VFW59_07420"
     CDS             complement(3287..4183)
                     /gene="rsgA"
                     /locus_tag="VFW59_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX5364083.1"
                     /translation="MSAILHGRIFAAFGRQYLARLDDGSEIACLTRGKKSEVVCGDEV
                     EIKRTGDASSVDGSGSQGVIERILPRRSLLHRSDAFREKYIAANVTQIIVVVAIEPGF
                     SDELLARCLIAAFDQKLEVLIVLNKCDLKTANNTAQRQLAPYRAIGYRVLELSAKQDV
                     SALRPYLHGHTSVLVGQSGMGKSTLINALLPEAHAATREISTALDSGKHTTTHARLYP
                     LDESSSLIDCPGVQAFGLHHLSFGEIEQGFAEFAHYLGQCRFRDCHHLHEPGCALREA
                     VAAGKIDARRLELFHQITANKA"
BASE COUNT          778 a         1301 c         1465 g          843 t
ORIGIN      
        1 ttctcattgg ggatgaatat ttccgcctga atttccggct tcatccatcc agagatgggg
       61 cacgccaagc gctgcgagcc actgcttacc agcgttgcct tttagcatcg ccatcgtcgc
      121 gacactgccc gcgaccaggc attgttcgct gatgacgctc accgaagcca ggccctgcac
      181 cggccagccg gtagagggat cgaggaggtg gcagtaacgt actccgtcca ccacgatgta
      241 acgctcgtag tcgccgctgg aggcgatggc accccgcgag atctcgacgg tggtcatgat
      301 gccatccgcc agtctgggat ggcgtatgcc gacgatccag ggcgagaggt cgggatgcgg
      361 gccgatcacc tggatgtcgc cgcccagttc gatcagaccg tgttcgatgc cctgcgcaac
      421 acagacggct gcggcctgat cggcggcgta ttccttggcg atgccgccga agtcgagttc
      481 catgccgggc aggtcgaacg atagccgggg gcgctcccag cgaatcttgt gcaggccgac
      541 cagcggcagc aaggcgtcga gttgccgtgg ttcgggaagg cgagacgcat cgaagtccca
      601 ggcccggcgc agtacgcccg aggtgatgtc gaacaggccg tcgcttttcg cgtggcaggc
      661 ataggcgtag tcgagcagcg tggcggtctc ttcgtctatc ggcagggatg tcccttgcgc
      721 agctgcccgg ttgatcttcg caagaatgct gtcgtcgcgg tatcgggaat agcgcgcttc
      781 gatgcgcagc acctcgtcga tcgcgcattg tgcggcgctc gatgccgccg actcatcccc
      841 cgcatacagc tgcaattcac actgcgagcc catcgcgaag aacgaaaaac ggtgcagcgc
      901 gggtgccatg gcggtgtttt caggcggccc ggcgttggaa gtcttccatg aatccggcca
      961 gttgttcgac tgcggacagg gtcagcgcgt tgtagatcga ggcgcggatg ccgccgatgg
     1021 cgcgatgccc cgccagcccg gagaacccgg ctgcctgagt ctcggtcagg aagcgttgct
     1081 gcaattcggg cgagggcagg ttgaacacca cgttcatcag cgacctgtcc ggtacggcgg
     1141 cgcggccgcg ataaaagccg tcactgccgt ccagcatgcg gtacagcagc tccgccttgc
     1201 cgtggttgat ttcggccatg cgcgcgacgc cgccgatctc gttcagcagc cagcgcgaga
     1261 ccagcagcac cacgtagatc gaaaagaccg gcggggtgtt gaaattggaa tgcgcctgaa
     1321 tgtggctgcg gtaattgagg aagccgggca ggtcggtgtt cgcgtccttg agcagctcgt
     1381 cgcgcaccag caccacggtg acaccggccg ggccgatgtt cttctgcgca tgggcataga
     1441 tcagcgagaa gcgctccgca tcaacggggt gcgacagaaa atccgaggac atgtcgcaga
     1501 cgcgcggcac atcgtcgcgg cccagcacgc gatggaactg cagcccctcg accgtctcgt
     1561 tggagatgta gtggaggtag ggcgcgtccg ccgagaaggc cagctcggcg tcggccggca
     1621 gtctgctgta accgcattcc gctccgctcc agatcacctg tatcgggcct tcgcgcttcg
     1681 cttccgggat ggccttgccg ctccagtagc cggtgtgcag gtattgcgcc gcatgcgact
     1741 ttccgcgcag cagggtcatc ggcaccatgg aaaactgctg cgtcgcgccg ccctgcaaaa
     1801 acagcacgtg atagttgtcg gacacgccga gcagcgtgcg gatgttgttt tccagttcgg
     1861 tgatgacggc ggcgaaccag tcggaacggt ggctgatgcc caggatcgac agcccgactt
     1921 cgggcacttc gagtatggct tcctgtacct ggcgcaatac gctctccggc aaggcgccgg
     1981 gaccgccgga aaaattcaga ctgttgagtg gtttcatctg tcggtaggtt cccagatcat
     2041 gttcaggacg gcttcgtggc tgcgaactcg gcagcccggc ggaaaagaaa gtcgcggaag
     2101 gcatccctga ccttccgcat atggaattgc ttgtagggga acagctcttc cggatccatg
     2161 gcgtgcacaa ccatgacatg gtcgacctcg aatatcagca gctggccatc ctgcgtctcc
     2221 gcgcagtcga tgcagaggta atcgagcccg gtgcgttcgt gtatggcttt cagcgcctca
     2281 tggtggcgtt gcgcgaagtc gtcgaaattc gccatgaagg cggcttcctc ttcccgcttg
     2341 tgcgcatcct catacatgcc cgcattcacg taatgcacca tccagtgcga cgacacgccc
     2401 atgtggcagg catagggtac gccgtcgatc agtgcaaccc ggaacttgcg gaactggccg
     2461 tccgtaccgc tgtaatcgat gaagggcgac aggaagaagt cttctgcatt gacccgggct
     2521 agatcgagcg cgattcctgc cgggtcggtg atgcggtcga gatcgcgtcc ggcctgcgag
     2581 ccgacgggac gcaggatgat ggggaagtcg caccctcccg atagttcggg caggctggat
     2641 tcgcccgcag cgatctccag cagcaccggg cgcgaggcgt gcagcgtcgg cgggattacc
     2701 aggcccggca catcctgcag cagcgcgctg gcgacggcgc gttccgtggt gcggatgtac
     2761 aggggggcgt tgatcaccgg tttgggccag tgggccaggg ccagctccag cgcttccagc
     2821 gtggggtggt tgtcgtcgga ttcgccgatg gcgacgatca ccgcatcgtg ctcgggcagc
     2881 ggctgggcca gcggatcgcc gggggtgacg taatagcaat ccagatcgat atcgccgtct
     2941 tcgagcaggc attcgagcgg cgtgttggcg gacaggtcgc ccggcaccat cagcatcagc
     3001 aggcggaatc tggccggttg tatggttgcc gcgatgtggt agatgcgttt gagcgccagc
     3061 gcctgttgct gcatggccat gccgagatcg cgctgcccca gacactgcat ggccagagac
     3121 aggttcatcc acaggttcgc atcgtcgcca ttgtcttggg cgagcgccag caattgctgt
     3181 gcgacgggct gtagatcggc gcctgcgatg ctcagtcgca gaaaaggagc cagcccgcga
     3241 aagggcgtct cggacggatg catgtgatcg ttgtcgttca ttgagcctaa gccttgtttg
     3301 cggtgatctg gtggaacagc tccagccgcc ttgcatcgat cttgcctgct gccacggctt
     3361 cgcgcaatgc gcagcccggt tcatgcaggt gatggcagtc gcgaaagcgg cactggccga
     3421 gatagtgtgc gaactccgcg aaaccctgtt cgatctcgcc gaagctcagg tgatgcaggc
     3481 cgaaagcctg cacgccgggg cagtcgatca gcgaactgct ctcatccagc ggatacaggc
     3541 gcgcatgggt ggtggtgtgt tttccggaat ccagcgcggt ggagatttcg cgggtagcgg
     3601 catgcgcctc cggcagcaag gcgttgatca gcgtggactt gcccatgccg gactggccga
     3661 ccagcacgct ggtgtggccg tgcaggtatg ggcgcagcgc ggagacgtcc tgttttgcgc
     3721 tgagctccag cacgcggtag ccgatggcgc gatagggcgc gagctggcgt tgcgcggtat
     3781 tgttggcggt tttcagatcg catttgttca gcacgatcag cacctccagc ttctggtcga
     3841 aggcggcgat caggcagcgt gccagcagct cgtcgctgaa gcccggttcg atcgcgacga
     3901 ccacgatgat ctgggtgacg ttcgccgcga tatatttttc gcggaaggca tcggagcggt
     3961 gcagcagcga gcggcgcggc aggatgcgtt cgatcacgcc ctgcgaaccg ctgccgtcca
     4021 cgctggaggc atcgccggtg cgtttgattt cgacttcgtc accgcacacc acttcgctct
     4081 tcttgccgcg cgtcagacag gctatctcgc tgccgtcgtc gagacgggcc agatattggc
     4141 gcccgaaggc ggcgaagatg cggccgtgaa gaatggcgct caaagcgggg acggcgcgtg
     4201 catggtcgcg gcagggttca tgaggtttat ttcagctggt agtaacgatt gagataggcg
     4261 ccgaggtgct gctcgtcctg agccgacagg gtgatgccga gattgccgga gcacatgccc
     4321 atctgctcga ccagttgcgc cgcgttgtgc accttgcggt cggcgcgggt gaatatcgcg
     4381 ctgccgt
//
LOCUS       DASUIV010000204         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_605908, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000204 DASUIV010000000
VERSION     DASUIV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605908"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1184)
                     /locus_tag="VFW59_07425"
     CDS             complement(3..1184)
                     /locus_tag="VFW59_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5364084.1"
                     /translation="MRLRYGAMPAAYGALLLALLAGSAHAGQQDELNNLRKRIGALQQ
                     ELEKTSESKSEVADALRESERAISDSNRELATLSTQQREADQKLGALQSDAQRLSRNI
                     TGQQSQLGELLYQQYLGGRQDYLKLILNNQNPNQVARELTYYQYIARNRARWLNSMRG
                     DLDALNAVSIATREQREKLATLRAEQLSQKKILEQEQRTRKQMLGSISEQLRQQRREI
                     GRLQRNEQRLSQLVEKIAKMLAKPKSRSLFRNNDLPDNRFDGKPFEQLKGKLTLPVTG
                     TVANRFGDTRPDSTVRWKGLFLRTSSGQNVKVVASGRVVFADWLRGFGNLIIVDHGNG
                     YMSLYGNNETLYKQVGDVLRGGDTLASVGNSGGNEDSGLYFELRHESKPLNPVDWLAA
                     R"
     gene            complement(1181..2710)
                     /gene="gpmI"
                     /locus_tag="VFW59_07430"
     CDS             complement(1181..2710)
                     /gene="gpmI"
                     /locus_tag="VFW59_07430"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5364085.1"
                     /translation="MKITPVLLLILDGFGYREEAESNAVMAARKPNFDKLWRDYPHTL
                     IQASEKHVGLPSHQMGNSEVGHLNIGAGRVVYQELSRVDVAIEDGSFYTNPALSAAVA
                     QAKQNDGALHILGLLSAGGVHSHESHIFAMLEMAARAGLKKIYLHPFLDGRDTPPRSA
                     AHSLQLLQQKCDELGVGQIAGIVGRFYAMDRDNRWERVQLAYDLLTQGRAEFTAGDAL
                     SALEQAYARDESDEFVKPTAITGTAMQDGDVAVFMNFRADRAREITRALTDEQFDGFT
                     RTRFPKLASFVTLSSYGEDFHLPCAYTPEAIHNGFGEYLSNLGLKQLRIAETEKYAHV
                     TYFFNGGKEQPYPGEDRILVPSPKVATYDLQPEMSAFEVTDKLVAAIQSRQYQAIICN
                     YANGDMVGHSGDMNAATRAIEALDICIGRVVEAMQSIGGEVILTADHGNAEQMADPAT
                     HQAHTAHTLNPVPFLYIGRKAVLIEGGALRDLAPSLLTMMGLPQPAEMTGHSLINLQH
                     K"
     gene            2804..3106
                     /locus_tag="VFW59_07435"
     CDS             2804..3106
                     /locus_tag="VFW59_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5364086.1"
                     /translation="MGERLIDREEDIQLAAQAIKAIAHPLRLKILCVLGDQEISVQDI
                     VEHVGTSQSNISQHLAILRDKGVLATRKDANRVFYRISDLRTLKLVVMMREVFCTV"
     gene            3171..>3356
                     /locus_tag="VFW59_07440"
     CDS             3171..>3356
                     /locus_tag="VFW59_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5364087.1"
                     /translation="MKNVLIGGVLLMGMVAAQAAEQLAVAPVQYREVAQTYSADGMVE
                     AVRQSTVSAQIGGRVKEI"
BASE COUNT          549 a          916 c         1128 g          763 t
ORIGIN      
        1 cgttatcttg ccgccagcca atcgaccgga ttgagcggtt tgctctcgtg gcgcagttca
       61 aagtataaac cggaatcttc gttcccgccg ctgttcccga cggaggccag cgtgtcgccg
      121 ccgcgcagca catcccccac ctgtttgtag agcgtctcgt tgttgccgta caggctcatg
      181 tagccgttgc cgtggtcgac gatgatcagg ttgccgaagc cgcgcaacca gtcggcgaac
      241 accacccggc cggaggcaac gactttgaca ttctgtccgc tggaagttct gaggaacaat
      301 cctttccagc gcaccgtgct gtcggggcgg gtatcgccga agcggttggc gacggtgccg
      361 gtgaccggca gcgtcagctt gcccttgagc tgttcaaagg gtttgccgtc gaagcggttg
      421 tccggcaggt cgttgttgcg gaacagcgag cgcgatttcg gcttggcgag catcttggcg
      481 atcttctcga ccagctggga gagccgctgc tcgttgcgtt gcaggcggcc gatctcgcgg
      541 cgttgctggc gcaattgttc cgagatgctg cccagcatct gtttgcgagt gcgctgttcc
      601 tgttccagta tctttttttg cgacagttgt tcggcgcgca gcgtcgccag tttttcgcgc
      661 tgttcgcggg tggcgatgct gaccgcattc agggcgtcca ggtcgccgcg catgctgttg
      721 agccagcgcg cacggttgcg cgcgatgtat tggtagtagg tcagttcgcg tgcgacctga
      781 ttggggttct ggttgttgag aatcagcttg agataatcct gcctgccgcc gaggtattgc
      841 tggtaaagca gttcgccgag ctgggactgc tggccggtga tgttccggct caggcgctgc
      901 gcatcgcttt gcagcgcgcc gagtttttgg tcggcttcgc gctgctgggt ggagagcgtg
      961 gcgagttcgc ggttgctgtc gctgatggcg cgttccgatt cgcgcagtgc gtccgccact
     1021 tcggatttgg attcgctggt cttttccagc tcctgctgca gcgcgccgat gcgcttgcgc
     1081 aggttgttca gctcatcctg ctggcccgca tgtgcgctgc ctgccagcag cgccagcagc
     1141 agtgcgccat aagccgcagg cattgcgccg tatcgcagcc tcatttgtgc tgcagattta
     1201 tcaggctgtg cccggtcatc tctgcgggtt gcggcaggcc catcatggtc agcaggctgg
     1261 gcgcgagatc gcgcagtgcg ccgccttcga tcagcactgc cttgcgcccg atatacagga
     1321 acggcaccgg attcagcgtg tgcgcggtgt gcgcctgatg ggtggccgga tcggccatct
     1381 gttccgcgtt gccatggtcg gcggtgagaa tgacttcgcc gccgatggac tgcattgcct
     1441 cgaccacgcg gccgatgcag atgtccagtg cctcgatggc gcgggtggca gcattcatgt
     1501 cgccgctgtg accgaccatg tcgccgttcg catagttgca gatgatggcc tggtactggc
     1561 ggctctggat cgcggcgacc agcttgtcgg tcacttcgaa ggcgctcatc tcgggctgga
     1621 ggtcgtaggt ggcgaccttg ggcgatggca ccagaatgcg gtcttcgccg ggatagggct
     1681 gctctttgcc gccgttgaag aaataggtca catgcgcata tttctcggtc tcggcgatgc
     1741 gcagctgctt taagcccaga ttggaaaggt attcgccgaa gccgttatgg atggcttcgg
     1801 gcgtgtaggc gcagggcaga tggaagtctt cgccgtagct gctcagcgtg acgaagctgg
     1861 cgagtttcgg gaagcgggtg cgcgtgaagc cgtcgaactg ctcgtcggtc agcgcgcggg
     1921 tgatctcgcg tgcgcggtcg gcgcggaagt tcatgaacac ggctacgtct ccgtcctgca
     1981 ttgcggtgcc ggtgattgcg gtcggtttga cgaactcgtc ggattcgtcg cgggcatagg
     2041 cctgttccag cgcggacagc gcgtcgccgg cggtaaattc cgcacggcct tgcgtgagca
     2101 ggtcgtaggc gagctgcacg cgctcccagc ggttgtctct gtccatcgca tagaagcgac
     2161 cgacgatgcc ggcgatctgg ccgacaccca gttcgtcgca tttttgctgc aacagttgca
     2221 gcgaatgagc cgcgcttctg ggcggcgtgt cgcgaccgtc caggaagggg tgcaggtaaa
     2281 tcttcttcag accggcgcgc gccgccatct ccagcatcgc gaagatgtgg ctctcgtggc
     2341 tgtgcacgcc gccggcggac agcaggccca gaatgtgcag cgcgccgtcg ttctgcttgg
     2401 cctgtgcgac ggcggcgctc agcgccgggt tggtgtagaa actgccgtcc tcgatggcga
     2461 cgtccacgcg gctgagttcc tgatagacca cacgacccgc gccgatgttg aggtgcccga
     2521 cctcggagtt gcccatctga tgcgaaggca ggccgacgtg tttttccgag gcttggatca
     2581 gggtgtgcgg gtagtcgcgc cacaatttgt cgaaattggg tttgcgcgcc gccataacgg
     2641 cgttggattc ggcttcttcg cggtaaccaa agccatcgag aatgagcagg agaacgggag
     2701 tgatcttcat tgatgtaata tgtggggcgc gtcgttttaa acagcgctat tttatctgaa
     2761 ttcaatgccc ttggtgtgaa gcacggggtg agacggggga atcgtgggcg agaggttgat
     2821 cgacagggaa gaagatatac aactggcggc gcaggccatc aaggcgatcg cgcatccgtt
     2881 gcgcctgaag atattgtgcg tgctgggcga tcaggagatc agcgtgcagg acatcgtcga
     2941 gcatgtgggg acttcgcaaa gcaacatatc gcagcatctg gcgatattgc gcgacaaggg
     3001 ggtgctcgcg acccgcaagg atgccaaccg ggtgttctat cgcatcagcg atttgcgcac
     3061 gctcaagctg gtggtgatga tgcgcgaggt gttctgcacg gtttaggacc ttgttttttt
     3121 gtgtttatta ggatatccta ataaacgtaa ttaattatta aggtggtctg atgaaaaatg
     3181 tcctgatcgg cggtgtgttg ttgatgggta tggtcgctgc gcaggcggca gagcagcttg
     3241 ccgtcgcacc ggtgcaatat cgcgaggttg cgcagaccta tagcgccgac ggcatggtcg
     3301 aggcggtgcg tcagtccacg gtgtccgcgc agatcggcgg tcgcgtgaag gaaatt
//
LOCUS       DASUIV010000205         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1259063, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000205 DASUIV010000000
VERSION     DASUIV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259063"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFW59_07445"
     CDS             <1..278
                     /locus_tag="VFW59_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364088.1"
                     /translation="DVAGSRDGDFHCISSSKNEACIMPQNRGRRQRGSLIETQVPLTD
                     LAGGLRYYLPARCNDVEAIAEGCCELSADTHRPDGSALAENQDERTI"
     gene            262..1329
                     /locus_tag="VFW59_07450"
     CDS             262..1329
                     /locus_tag="VFW59_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5364089.1"
                     /translation="MSGQSSKNGSIVIVDDNPDNLRVLSGVLEGAGFVVRPAISGEIA
                     LSSIRRNPPDLVLLDIRMPGMDGYETCRQLKDDPLTRDIPVIFISALQETGDKVAAFS
                     AGGVDYVTKPFQFEEVLARVRVHLELHHIKLHLDDIVTAKTEALRETMASLLASQQKY
                     RDLLEQIIDSFGLAVEYRDPYTAGHQRRVAELAGAIATEMGMQSDALEGLRLGALVHD
                     LGKIRVPSEILNRPGKLGELEMGLIRTHSQTGTDILHGVNFSRPVAQMVMQHHERIDG
                     SGYPKGLKGDEILLEARIIAVSDVVEAMVSHRPYRAALGVEKALEEIREGSGRLYDPE
                     VVGVCLELFEQRDFKFSELAF"
     gene            complement(1343..1732)
                     /locus_tag="VFW59_07455"
     CDS             complement(1343..1732)
                     /locus_tag="VFW59_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX5364090.1"
                     /translation="MTDINFKREISATLDEAVERATKALGAEGFGILTRIDMHAKIKE
                     KTGKDIVPTVILGACNPNLAYEAYTANSDVASLLPCNAVVREIAPGRVSLEFAKPTGM
                     MKMLGDEKLVALAAEADVKIARALASI"
     gene            complement(1906..2406)
                     /locus_tag="VFW59_07460"
     CDS             complement(1906..2406)
                     /locus_tag="VFW59_07460"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX5364091.1"
                     /translation="MTTLRALQSDITHLKVDAIVNAANSSLLGGGGVDGAIHRAAGPE
                     LLAECAALGGCPTGEARLTQGYRLPAKFVIHTVGPVWHGGEHNEAQLLASCYRESLKL
                     AAAHQLNSIAFPCISTGVYGYPAALAAEVAIAAVRDFLRAPCSLQEVIFCCHSASDLA
                     LYQARL"
BASE COUNT          487 a          739 c          830 g          488 t
ORIGIN      
        1 ccgacgtagc cggttcccgc gatggcgatt ttcattgcat cagtagttca aaaaacgagg
       61 cgtgcattat gccgcagaac agggggcggc gtcagcgggg cagtctgatc gaaactcagg
      121 ttccgctgac tgaccttgcc ggtggcttgc ggtactattt gccggcccgc tgcaacgatg
      181 tcgaggcaat agcggagggg tgttgcgaat tatctgccga tacacatcga ccagacggtt
      241 cggcgttagc ggagaatcaa gatgagcgga caatctagca agaacggctc catcgtcatc
      301 gtcgatgaca atccggacaa tctgcgcgtg ttgagcggcg tgctggaggg cgcgggtttt
      361 gtcgtgcgtc ccgcgatcag cggcgagatc gcgctgagtt cgataaggcg gaatccgccc
      421 gatctggtgc tgctggacat ccgcatgccc ggcatggacg gttacgagac ctgccgccag
      481 ctgaaggacg acccgctcac ccgcgacatc ccggtgatct tcatcagcgc gctgcaggag
      541 accggcgaca aggtggcggc cttctcggca ggcggggtgg attacgtgac caaaccgttc
      601 cagttcgagg aggtgctggc gcgggtgcgg gtgcatctgg agctgcatca catcaaactt
      661 catctggacg acatcgtcac ggcgaaaacc gaggctttgc gcgaaaccat ggcgtcgctg
      721 ctcgcgagcc agcagaaata ccgcgatttg ctggagcaga tcatcgattc cttcggtctg
      781 gcggtcgaat atcgcgaccc ctataccgcc gggcaccagc gccgcgtcgc cgaactggca
      841 ggcgccatcg ccaccgagat gggcatgcag agcgatgcgc tggaagggtt gcgcctgggc
      901 gcgctggtgc acgatctggg caagattcgc gtgccgtcgg agatactcaa tcgtcccgga
      961 aagctgggcg agctggagat ggggctgatc cggacccact cccagaccgg caccgacatc
     1021 ctgcatggcg tgaacttctc ccggccggtc gcgcagatgg tgatgcagca tcacgagcgc
     1081 atcgacggca gcggctatcc gaaggggttg aagggggatg aaatcctgct ggaagcccgc
     1141 atcatcgcgg tgtccgacgt ggtcgaggcg atggtctcgc atcgccccta tcgcgccgcg
     1201 ctcggcgtgg aaaaggcgct cgaagaaatc cgcgagggta gcggcaggct ctatgacccc
     1261 gaggtggtcg gggtctgcct ggagctgttc gaacagcgcg acttcaagtt cagcgaactg
     1321 gcgttctagg caccggcgag cttcagatgc tggccagcgc gcgggctatc ttcacatcgg
     1381 cttcggctgc cagcgccacc agtttttcgt ctcccagcat cttcatcatg cccgtaggct
     1441 tggcaaactc cagcgagacc cggcccggcg cgatctcgcg caccaccgca ttgcagggca
     1501 gcaggctcgc cacgtcgctg ttcgcggtgt aagcctcgta ggccagattc ggattgcacg
     1561 cgccgaggat caccgtcggc acgatgtcct tgccggtctt ctccttgatc tttgcatgca
     1621 tgtcgatgcg ggtcaggatg ccgaagccct ctgcgcccag cgccttggtg gcgcgttcga
     1681 cggcctcgtc cagcgtggcg gaaatttcgc gtttgaagtt gatgtcggtc atggtgttcc
     1741 tccgttcggt gatgtgaaat aaaaaacagc catcagcaga ttacacagat tacgcaagat
     1801 taaacaatcg ggttgtgaac tttggtctgt tactcggttg agccaggatg ctagcagagt
     1861 gaagcggttt ggaatctgcg caatctgtgg attcaggttg ttcctttaca gtcgcgcctg
     1921 gtacagcgcc agatcgctgg ccgaatggca gcagaagatc acctcctgca acgaacaggg
     1981 cgcgcgcaga aaatcgcgca ccgccgcaat cgcgacctcg gccgccaggg ctgccggata
     2041 gccatatacg ccagtgctga tgcaggggaa ggcgatgctg ttcagttgat gtgcggcggc
     2101 cagcttcagg ctctcgcggt agcaggaggc cagcagctgc gcctcgttgt gctcgccgcc
     2161 atgccacacc ggccccacgg tatggattac aaattttgcg ggcagccggt agccctgggt
     2221 gagccgcgct tctccggtcg ggcagccgcc cagcgcagcg cattccgcca gcagctccgg
     2281 ccccgccgct ctatggatcg cgccgtccac gccgcccccg ccgagcagcg aggagttcgc
     2341 cgcgttgacg atcgcgtcga ccttgaggtg ggtgatgtcg gactggaggg cgcggagggt
     2401 ggtcatagtg tgcctctgtg tgatgttgag agggaaggtg caagcattat tgcgccggat
     2461 aacatcaaaa tcaaaaccgt ggggggtagg atgggttgag cgaaagagat acccatcgaa
     2521 aaccgtcggg ggttgcccga cagc
//
LOCUS       DASUIV010000206         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_607062, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000206 DASUIV010000000
VERSION     DASUIV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607062"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VFW59_07465"
     CDS             <1..1006
                     /locus_tag="VFW59_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5364092.1"
                     /translation="DPDKVVAGNAPFELHPVGEPQSGKPYRRGVLLTHGLSDSPYFMR
                     HLAAFFQRNGFRVMVPLLPGHGTQPGDLLDLHWQEWAKTVRYGADRLSAEVDELYLAG
                     YSAGAALSLLQSAQDERVRGLFLFAPALQIDARARWANMHRLYSGLLPQAAWLSLMPD
                     RDLYKYESFCKNAAAQMYALTQALPQRGCDIPVFAAASADDATVHAAATLRFMRQACH
                     PHRHLLWYATQPPQVADERVEWIDSALPGQRILSSAHTAIVLAPEDAYYGGEYVNCLH
                     YYPHDMPRYTQCSETSRDVWRGEVTGENLQRGLLRRLMVNPHYAAMEKSMQRFIDGLP
                     "
     gene            complement(1019..1438)
                     /locus_tag="VFW59_07470"
     CDS             complement(1019..1438)
                     /locus_tag="VFW59_07470"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HEX5364093.1"
                     /translation="MSEIDPSTIKAYRETNYYIAAPAPFVLRVGVTSEALARICAQHR
                     TDCCAFVTASNPYSRLVDDQENADRQAGLARELERRGLTFFHGTGRHPSGSWPAESSY
                     LVLGLSLADAKMLGEAYQQNAILWCGPDAVPELILLR"
     gene            complement(1513..2550)
                     /locus_tag="VFW59_07475"
     CDS             complement(1513..2550)
                     /locus_tag="VFW59_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEX5364094.1"
                     /translation="MNFQQLRIIREAVRCNFNLTEVGNALFTSQSGVSKHIKDLEDEL
                     GVELFVRKGKRLLGLTDPGRELLEIVERILLDANNIKNLVEQYSKRDEGQLTVIATHT
                     QARYSLPQVITEFKKTFPKVHLKLHQAGPAEIVTMLLNGEADIGVATEALADVTRLDS
                     FPYYSWHHAVIVPKGHPLTRLMDAEGASPLLNPLPQAGEEANERYAASSFSGLTLKAL
                     AEYPIVTYHEGLTGRAKIDQSFADAGIAPDIAMSALDADVIKTYVELGLGIGIIASMA
                     FNPAKDSDLRLLRCDHLFGANTTYIALRRGHYLRSFAYRFIELCSPALDETTIRAGLT
                     TLRVKDFDERP"
     gene            complement(2531..>3255)
                     /locus_tag="VFW59_07480"
     CDS             complement(2531..>3255)
                     /locus_tag="VFW59_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX5364095.1"
                     /translation="HNEAEIKRRVHELLSLVQLDWFAERYPAQLSGGQRQRIALARAL
                     AVEPKILLLDEPFGALDAQVRKELRRWLRRLHDELHITSIFVTHDQEEALEVADRVVV
                     MNKGRIEQVGTPDEVYAAPATPFVYQFIGNVNLFHSRDHAPWTEQHGDAVAYVRPHDI
                     DISVTPQDDTALPVEVKLVRAIGSIVRVEVAADGSSELIEIELSRERFDGAPLASGDK
                     VFIRPRQFRVFETGEQNELSAA"
BASE COUNT          596 a          988 c         1025 g          646 t
ORIGIN      
        1 agatccggac aaggtcgtcg ccgggaatgc gccgttcgag ctgcatcccg tcggagagcc
       61 gcaaagcggc aagccttacc ggcgcggtgt gctgctgacg catggactgt ccgattctcc
      121 ctatttcatg cgccatctcg cggcattctt tcagcgcaac ggtttccggg tgatggtgcc
      181 gttgttgccg ggacacggca cgcagccggg cgatctgctc gacctgcact ggcaggaatg
      241 ggcgaagacg gtgcgctacg gagcggatcg tctgtccgcc gaagtggacg agctctatct
      301 ggcgggctat tcggcaggcg ctgcattgag cctgttgcag agcgcgcagg acgagcgtgt
      361 gcgcggcctg tttctgttcg ctcctgcact gcagatcgat gcccgcgcac gctgggcgaa
      421 tatgcaccgg ctgtatagcg ggctgttgcc gcaggcggca tggctgagtc tcatgcccga
      481 ccgcgatctc tataaatacg aatcgttctg caagaatgcc gccgcgcaga tgtatgcgct
      541 gacgcaggcc ttgccgcaac gcggctgcga cattccggtg ttcgccgcag ccagcgccga
      601 cgatgcgacg gtgcatgctg cggcgacgct gcgcttcatg cggcaggcat gccatccgca
      661 tcgccatctg ctgtggtatg cgacgcagcc gccgcaagtc gcggatgagc gcgtcgaatg
      721 gatcgacagc gcattgcccg ggcaacgcat cttgagcagc gcgcacaccg ctatcgtatt
      781 ggcgcccgag gatgcgtatt acggcgggga atatgtgaac tgcctgcatt actacccgca
      841 tgacatgccg cgctacacgc aatgcagtga aacttcgcgc gatgtctggc ggggagaagt
      901 gaccggggaa aatctgcagc gcggtctgtt gcggcgtctg atggtcaatc cgcactacgc
      961 ggcgatggag aaatccatgc agcgctttat cgatggcttg ccctgatcgt tgttgaactt
     1021 atctgagcag gatcaattcc ggcacggcat cggggccgca ccagaggatc gcattttgtt
     1081 ggtatgcctc gcccagcatt tttgcatcgg caagcgataa ccccagcacc agataactgc
     1141 tctccgcagg ccagctccca gacggatggc ggccggtgcc gtggaagaag gtcaggccgc
     1201 gccgctccag ttcacgcgca agcccggcct gacggtcggc attctcctga tcgtcgacaa
     1261 ggcggctata gggattgctg gcggtgacga aggcgcagca gtcggtgcga tgttgcgcgc
     1321 agatccgggc cagcgcctcg ctggtgacgc cgacccggag tacgaacggc gcaggcgccg
     1381 cgatgtaata gttcgtctcc cgataagcct tgatggtcga tggatcgatt tctgacatgg
     1441 cgcatgttgg ataagaagtc tgatcgtatt ctacggcgat gaaccgtggc cggcatcggc
     1501 gcccgaaata aattacggcc tttcgtcgaa gtcctttact cgaagcgtcg tcaggcctgc
     1561 gcgtatggtg gtctcgtcca gggccgggga gcacagttcg atgaagcggt aggcgaagct
     1621 gcgcaaatag tgcccgcgcc gcaacgcgat ataagtcgta ttcgcaccga acaggtgatc
     1681 gcatctcaac aggcgcaggt cgctatcctt ggccgggttg aaagccatcg aagcgatgat
     1741 gccgataccc agacccagtt ccacataagt cttgatcaca tccgcatcca gcgcggacat
     1801 ggcgatatcc ggcgcgatgc ccgcatcggc gaacgactgg tcgatcttgg cgcggccggt
     1861 caagccttcg tgataagtga cgatgggata ctcggccagc gccttcaagg tcagcccact
     1921 aaatgaagaa gccgcgtagc gttcgttcgc ttcctctccc gcttgcggga gaggattgag
     1981 gagaggggaa gcgccctcag catccattaa ccgtgttaat ggatgccctt tcggcacgat
     2041 caccgcatgg tgccacgagt aatagggaaa cgaatcgagc cgggtgacgt cggccagcgc
     2101 ctcggtcgcc accccgatgt ccgcctcgcc gttcagcagc atggtcacga tctcggcagg
     2161 tccggcctga tgcagcttga gatgcacctt gggaaaggtc ttcttgaact cggtgatcac
     2221 ctgcggcaag gagtagcgcg cctgcgtgtg ggtggcgatg acggtgagct ggccttcgtc
     2281 gcgcttgctg tactgctcca ccagattctt gatgttgttg gcatcgagca ggatacgctc
     2341 gacgatctcc agcaactcgc gtccgggatc ggtcagtcca agcagacgct tgcccttgcg
     2401 cacgaacagc tccacgccca gctcgtcttc gaggtctttg atgtgcttgg acacgccgga
     2461 ctgcgaggtg aacagcgcat tgcccacctc ggtcaggttg aagttgcaac gcaccgcctc
     2521 gcggatgatc ctaagctgct gaaagttcat tctgctctcc tgtctcgaac acgcggaact
     2581 ggcgcggccg gatgaatacc ttgtcgccgc tcgccagcgg cgcgccatcg aagcgttcgc
     2641 gggaaagttc gatctcgatc agctcgctgc tgccgtctgc cgccacttcc acgcgcacga
     2701 tcgagccgat ggcgcgcacc agtttaactt caaccggcag ggcggtgtcg tcctgcggcg
     2761 tgacgctgat gtcgatgtcg tgcgggcgca cataggccac cgcgtcgccg tgctgctcgg
     2821 tccaaggcgc atggtctcgg ctgtggaaca ggttcacgtt gccgatgaac tggtacacga
     2881 acggcgtggc gggcgcggca tacacctcat ccggcgtgcc cacctgttct atcctgccct
     2941 tgttcatcac caccacgcgg tcggccacct ccagcgcctc ttcctgatcg tgcgtgacga
     3001 agatgctggt gatgtgcagc tcgtcgtgca ggcggcgtag ccagcggcgc aactccttgc
     3061 gcacctgcgc atccagcgcg ccgaacggct cgtccagcag caaaattttc ggctcgacgg
     3121 cgagcgcgcg cgcgagtgcg atgcgctggc gctgcccgcc ggaaagctgc gcggggtaac
     3181 gttcggcgaa ccagtcgagt tgcaccaggc tcaacaactc gtgcacgcgg cgtttgatct
     3241 cggcttcatt gtggc
//
LOCUS       DASUIV010000207         5581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_516618, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000207 DASUIV010000000
VERSION     DASUIV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516618"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /gene="dsbD"
                     /locus_tag="VFW59_07485"
     CDS             complement(<1..1347)
                     /gene="dsbD"
                     /locus_tag="VFW59_07485"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEX5364096.1"
                     /translation="MRTLLLALMLIIAPFAHAGFLDSLGGAKQQTFLPPDQAFGLQVL
                     VRDAHTLQANFNVVEGYYLYRDKISFTSKKGSVRIKAVNLPHGEMKQDPSFGQVEVFH
                     RSFQVEIELDRLADGAGGVLLDASYMGCSEKGLCYPPIVKTLRIELPDAKTGLREPEL
                     TEAPSPAYIKPLPAGGNENSRIARLFAGGSFWLIVSSFFGAGLLLALTPCVFPMIPIL
                     SGIIVGRGHKITKTHAFVLSLAYVLGMAITYAAAGVAAGFSGNLISNALQTPWVLGSF
                     AAVFVVLSLSMFGFYELQLPNALQSKLTDASNRLHGGHLSGVFVMGALSAIIMGPCVA
                     APLAGALLYIGQTHDAVLGGTALFALAMGMGAPLLLIGSSAGVLLPRAGAWMEAVKRF
                     FGVLLLGLAIWIVSPVIPVGVQMALWAALLVFSGIYLRALDPLPHHSSGWSRLVKGV"
     gene            complement(1407..1724)
                     /gene="cutA"
                     /locus_tag="VFW59_07490"
     CDS             complement(1407..1724)
                     /gene="cutA"
                     /locus_tag="VFW59_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX5364097.1"
                     /translation="MEVLVVLTNLPDAASAERLARQLIEQRLAACVNQLAPCNSTYRW
                     QGEIETATEVPLLIKTTRAAYPLLEQAIRAAHPYELPEIVALPVAAGLPGYLAWVAQE
                     TGE"
     gene            complement(1724..2725)
                     /gene="holA"
                     /locus_tag="VFW59_07495"
     CDS             complement(1724..2725)
                     /gene="holA"
                     /locus_tag="VFW59_07495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5364098.1"
                     /translation="MQITSDDLPRHLAKGLAPLYVVYGDALLLAIEAADSIRAAARAA
                     GYSERETFVAEQHFRWGELRNSAQSLSLFAERKVIDLRIPSGKPGVEGGQALQDYVAN
                     MSPDILTLISLPKLDRTTQKSRWFAALESSAVMVTAEDIPRQAMPGWIAGRLKRQEQS
                     ASKDTLEFLGDRCEGNLLAAFQEIQKLALLFPAGELSFDQVKDAVMDVARYDIFKLSE
                     AMLGGDTARFVRILDGLRAEGTATVLVLWAISEDIRTLGKVLQATGRGGNIDGALREA
                     RVWGPRQGLIGNAARRFKFPHVERAMHQAARLDKTIKGLRQGDVWDELLQLGLRFTR"
     gene            complement(2728..3213)
                     /gene="lptE"
                     /locus_tag="VFW59_07500"
     CDS             complement(2728..3213)
                     /gene="lptE"
                     /locus_tag="VFW59_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX5364099.1"
                     /translation="MRARMFVLLTALLLGACGFHLRGQAGMPFATLYLDAPNTSFVAD
                     LRRGLEANNVKLLPSPEKADVVLNIVSETPEKRILSLGGSGRVTEYQLRYRVSLRAYD
                     NKQQEWIPAEEMELQRDYSYSDAAILAKESEEAMLYQSMRSDMVQQILRRLSHAKPQP
                     Q"
     gene            complement(3234..>5581)
                     /gene="leuS"
                     /locus_tag="VFW59_07505"
     CDS             complement(3234..>5581)
                     /gene="leuS"
                     /locus_tag="VFW59_07505"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5364100.1"
                     /translation="YSNIDYMRKQLQSLGLGIDWSREIATCKPDYYRWEQWLFTRLFE
                     KGVIYKKTSTVNWDPVDNTVLANEQVIDGRGWRSGALVEKREIPMYFFRITQYAEELL
                     ADLDQLDGWPEQVKTMQANWIGKSFGVRFAFEVCAEGESEALSPSPSPASGGGEQELL
                     WVYTTRADTIMGVTFVAVAAEHPLALRAAQGNPALAAFIEDCKHGSVAEADMATMEKK
                     GMDTGIKVTHPLTGEQVPVWVGNYVLMGYGEGAVMAVPAHDERDFGFAKKYDLPIKQS
                     IAVKDQAFSDDAWAEWYGDKDGVCVNSGKYDGLNYDQAIDAIAADLAAKGLGEKKTQF
                     RLRDWGISRQRYWGCPIPIIHCPSCGDVPVPDDQLPVVLPENVVPDGAGSPLAKMPEF
                     YECSCPKCGGAARRETDTMDTFAESSWYYARYASPNCTTGMVDKAAAAKWLPVDQYIG
                     GIEHAILHLLYARFFHKLMRDEGLVPGDEPFKNLLTQGMVIAPTFYRDAENGKKLWIN
                     PAEVDVATDERGRPTGAVLKADGLPVIIGGTEKMSKSKNNGVDPQAIIDEYGADTARL
                     FMMFAAPPDQSLEWSDAGVEGAFRFLKRVWKAAHDHVEQGVVAAYAGGELSAAAKALR
                     FQLHHTIAKVDDDLGRRKTFNTAIAANMELLNALAKFDEQTPAARAVTQEVLEAIVLM
                     LCPIVPHLSQALWTELRPGSDVLEQSFPVSDPAALVQDEIELVIQVNGKLRGSIRVAK
                     DADKATIERLALEHEAVQKQMAGAVAKKVVVVPGRLINIVV"
BASE COUNT         1056 a         1880 c         1649 g          996 t
ORIGIN      
        1 cacccccttg accagtcggc tccagccgct ggaatggtgc ggcagaggat ccagcgcccg
       61 cagatagatg ccggaaaaga ccagcagcgc cgcccacaac gccatctgga ccccgaccgg
      121 aatgaccggc gagacgatcc agatcgccaa ccccagcagc agcacaccga agaagcgttt
      181 gaccgcctcc atccacgcgc ccgctctggg cagcaatacg cccgccgagc tgccgatcag
      241 cagcagcggc gctcccatcc ccattgccag cgcgaacaag gcggtgccgc ccagcaccgc
      301 atcgtgggtc tgacctatat atagcaacgc acccgcaagc ggcgcggcca cgcaggggcc
      361 catgatgatg gccgacagcg cgcccatcac gaacaccccg gacagatggc cgccgtgcag
      421 cctgttgctg gcatcggtca gcttgctttg cagcgcgttg ggcaattgca gttcatagaa
      481 gccgaacatg gagagcgaca gcaccacgaa cacggcggcg aagctgccca gcacccaggg
      541 cgtctgcagg gcattggaaa tcagattacc ggagaagccc gccgccacac cggctgccgc
      601 ataggtaatc gccatcccca gcacataggc cagcgacagc acaaaggcat gggtcttggt
      661 gatcttgtga ccgcgcccga cgatgatgcc ggacaggatg ggaatcatcg gaaacacgca
      721 gggcgtcaat gccagcaaca accccgcacc gaagaaggac gacacgatca accagaagct
      781 gccgccggcg aacagcctgg cgatccggga attctcattg ccgccggcgg gcagcggctt
      841 gatgtaagcc ggtgacggcg cctcggtcag ctctggctca cgcaggccgg tcttggcatc
      901 gggcaattcg atccgcaacg tctttacaat cggcggatag cacaatccct tttcgctgca
      961 gcccatgtaa ctggcgtcca gcaagacacc gccggccccg tcggccaggc gatccagctc
     1021 gatctcgacc tggaacgaac ggtgaaacac ctcgacctga ccgaacgagg gatcctgctt
     1081 catttcgcca tgcggcagat tcaccgcctt gatgcgaacc gaacccttct tgctggtgaa
     1141 gctgatcttg tcgcgataga gatagtaacc ctcgaccacg ttgaaattgg cttgcagcgt
     1201 gtgtgcatcg cgcaccagca cttgcaggcc gaacgcctga tccggcggca aaaaggtctg
     1261 ctgtttggcg ccgcccaagc tgtcgagaaa accggcatgg gcaaacggtg cgatgatcaa
     1321 catcaatgcc aacaaaagag tacgcatgat ctagtccaaa taattcataa gaaaacctgc
     1381 aagccgctcc cacaatacat ccattgttat tccccggttt cctgcgccac ccatgccaga
     1441 tagccgggca agccagccgc aaccgggaga gcaacgatct cgggaagctc ataagggtga
     1501 gcggcgcgta tcgcctgttc gagaagcgga taggcggcgc gcgtggtctt gatcagcagc
     1561 ggcacctcgg tggccgtctc gatctcaccc tgccagcgat aagtcgagtt gcagggggca
     1621 agctgattga cgcaggccgc gaggcgctgc tcgatcaact gtcgcgccaa gcgctcggcg
     1681 gatgccgcat ccggcagatt ggtgagcacg accagcacct ccatcaccgg gtgaacctca
     1741 ggccgagttg caacagttca tcccacacat caccctgacg caggcccttg atggtcttgt
     1801 ccagccttgc ggcctgatgc atggcgcgct cgacatgcgg gaacttgaac cggcgcgcag
     1861 cattgccgat caagccctga cgcggccccc acacccgcgc ctcgcgcaac gcgccgtcga
     1921 tgttgccgcc gcgcccggtg gcttgcagca ccttgcccag cgtgcggata tcctcgctga
     1981 tcgcccacag caccagcacc gtggcggtgc cctcggcgcg cagaccgtcg aggatgcgga
     2041 caaagcgcgc ggtatcgccg cccagcatcg cctccgacaa tttgaagatg tcgtagcgcg
     2101 ccacgtccat caccgcgtcc ttgacctgat cgaacgacag ctcgcccgcc ggaaacagca
     2161 gtgcgagctt ctggatctcc tggaacgcgg cgagcagatt gccctcgcaa cggtcgccga
     2221 ggaattccag cgtatccttg ctggccgact gctcctgacg cttcaaccgt ccggcgatcc
     2281 agcccggcat ggcctgacgc ggaatatcct cggcggtgac catcacggcg ctgctttcca
     2341 gcgcggcgaa ccagcggctc ttctgtgtgg tgcgatcgag tttgggcagc gagatcaacg
     2401 tgaggatgtc cggactcatg ttggcgacgt agtcctgcaa cgcctgcccg ccttccacgc
     2461 cgggttttcc ggacggaatg cgcaggtcga tcaccttgcg ctcggcgaac aacgacaggc
     2521 tctgcgcact gttgcgcagc tcgccccatc tgaaatgctg ttcggcgaca aaggtctcgc
     2581 gctcgctgta acccgccgcc cttgcggcgg cgcggatgct gtccgcagcc tcgatcgcca
     2641 gcagcagcgc gtcgccatac accacgtaca gcggcgcaag gcccttggca aggtgacgcg
     2701 gcagatcgtc gctggtgatt tgcatgctta ttgtggctgg ggtttggcgt ggctcaggcg
     2761 gcgcaggatc tgctgcacca tgtcgctgcg catgctctgg tacaacatcg cctcttcgct
     2821 ctccttggcc agaatcgcgg catcgctgta agaataatcg cgctgcaact ccatctcctc
     2881 ggcggggatc cactcctgct gcttgttgtc gtaggcgcgc agcgagacgc gataacgcaa
     2941 ttgatactcc gtcacacgcc cgctcccgcc cagcgacagg atccgcttct cgggtgtctc
     3001 ggacacgata ttgagtacca catcggcctt ctctggcgac ggcagcagct tcacattatt
     3061 ggcctccaac ccccggcgca gatcggcgac gaaggaggtg ttgggtgcat ccagatacag
     3121 cgtcgcgaac ggcatgcccg cctgtccgcg cagatggaaa ccgcaggcgc cgagcaacaa
     3181 agcggtcaac aacacaaaca tccgtgcgcg catctcgttc tcccaaataa acactagacg
     3241 acgatattga tcaaacgacc cggcaccacc acgaccttct tcgccacagc ccctgccatc
     3301 tgtttctgca ccgcttcatg ttcgagggcc agccgctcga tggtcgcctt gtcggcatcc
     3361 ttggcgacgc ggatgctgcc gcgcagcttg ccgttcacct ggattaccag ctcgatctcg
     3421 tcctgcacca gcgcggccgg gtcgctcacc gggaaagact gctccagcac atcgctgccc
     3481 gggcgcagct ccgtccacag cgcctggctc aggtgcggca cgatcgggca gagcatcagc
     3541 acgatggctt ccagcacttc ctgcgtcacg gcacgcgcgg ctggcgtctg ttcatcgaac
     3601 ttcgccagcg cattgagcag ctccatgttg gccgcgatcg cggtattgaa ggtcttgcga
     3661 cgccccagat cgtcgtccac cttggcgatg gtgtggtgca gctggaagcg cagggccttg
     3721 gcagcggcac tgagttcgcc gcccgcatag gccgcgacca caccctgttc gacatggtcg
     3781 tgcgccgctt tccagacgcg cttgaggaag cggaatgcgc cctccacgcc cgcatccgac
     3841 cactccagcg actggtccgg cggagcggca aacatcatga acaggcgcgc ggtgtcggcg
     3901 ccgtattcgt cgatgatggc ctgcggatcg acgccgttgt tcttggactt ggacatcttc
     3961 tcggtgccgc cgatgatcac cggcaggccg tcggccttca gcaccgcacc ggtcgggcgg
     4021 ccgcgctcgt cggtcgccac gtccacctcg gccggattga tccacagctt cttgccgttc
     4081 tcggcatcgc gatagaaggt cggcgcgatc accatgccct gtgtcagcag gttcttgaac
     4141 ggctcgtcgc ccggcaccag cccctcgtcg cgcatcagct tgtggaagaa gcgcgcatac
     4201 aggaggtgca ggatcgcgtg ttcgatgccg ccgatgtact ggtccaccgg cagccatttg
     4261 gcggccgccg ctttgtccac catgccggtg gtgcaattcg ggctggcata gcgcgcgtaa
     4321 taccaggacg actccgcgaa ggtgtccatg gtatcggtct cgcgccgggc cgccccgccg
     4381 catttgggac agctgcactc gtagaactcg ggcatcttcg ccagcgggga acccgcgccg
     4441 tccggcacca cgttctcggg caacaccacc ggcaactggt cgtccggcac cggcacgtcg
     4501 ccgcaactcg ggcaatggat gatcggaatc gggcagcccc agtagcgctg gcgcgagatg
     4561 ccccagtcgc gcagacggaa ctgcgtcttc ttctcgccca ggcctttcgc ggccagatcg
     4621 gcggcgatcg catcgatggc ctgatcgtaa ttcaggccgt cgtacttgcc ggagttcacg
     4681 cacacgccgt ctttgtcgcc ataccactcg gcccaggcat catcgctgaa agcctgatcc
     4741 ttgacggcga tggactgctt gatcggcagg tcgtattttt tcgcaaagcc gaagtcgcgc
     4801 tcgtcatgag ccggaacggc catcaccgcg ccctcgccgt aacccatcag cacatagttg
     4861 cccacccaga ccggaacctg ttcgccggtc agcggatggg tcaccttgat gccggtgtcc
     4921 atgcccttct tctccatggt cgccatgtcg gcttcggcca cgctgccgtg cttgcaatcc
     4981 tcgatgaagg cggcgagcgc cggattgccc tgcgcggcgc gcagcgccag cggatgctcg
     5041 gcggccaccg cgacgaaggt cacgcccatg atggtgtcgg cgcgggtggt gtagacccac
     5101 agcaattctt gctcccctcc cccgctcgcg ggggaggggc tgggggagag ggcctcggat
     5161 tcgccttcgg cgcaaacttc aaacgcgaat cgcacgccga agctcttgcc gatccagttc
     5221 gcctgcatgg tcttgacctg ctccggccag ccgtcgagct gatcgaggtc ggccagcaac
     5281 tcttcggcat actgggtgat gcggaagaaa tacatcggga tttcgcgctt ctcgaccagc
     5341 gcgccggagc gccagccacg gccgtcgata acctgctcgt tcgccagcac ggtgttgtcc
     5401 accggatccc agttcaccgt ggaggtcttc ttatatatga cccccttctc gaacagacgg
     5461 gtaaacagcc actgctccca gcggtagtaa tccggcttgc aggtggcgat ctcgcgcgac
     5521 cagtcgatgc ccagccccag cgattgcagc tgcttgcgca tgtaatcgat gttggaatag
     5581 g
//
LOCUS       DASUIV010000208        17873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1260687, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000208 DASUIV010000000
VERSION     DASUIV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 17873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17873
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260687"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1436
                     /locus_tag="VFW59_07510"
     CDS             177..1436
                     /locus_tag="VFW59_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5364101.1"
                     /translation="MTGMMPGNDKTAQRTAGNDTIDSSGLLETNMTNINRPFALLLML
                     SALLSACNDKQATPPGGPGAGMPPPEVEVIEVDAGNATLTRDLTGRLQAYRTAQVRAR
                     VEGVIEKRLFKEGSEVKAGTSLYRIDARTYRATAEAARADLSIARQTVNRYRSLLDIK
                     AVSQQEYELAMGKFKQAEAALTRAQEDLENAEVPAPISGHIGRAAVTEGALVGKGEAT
                     LLATIEQLDPVYANFTQSSNDLLRLRQAIASGQLKRPDSAEVELLLEDGSVYPLKGRT
                     FFTDMAVDPSTGSVALRAEFANPQHALLPGMFVRVRFPEAVANNVIRVPQRAVLAGPQ
                     GQFVMVVGAEDKVRPQPVKTAGMVAGDFIITSGLTGGEQVIVNGLQKARPGTVVKPVP
                     LGGATSPAASAPAAIPAASSTAATGKQ"
     gene            1446..4562
                     /locus_tag="VFW59_07515"
     CDS             1446..4562
                     /locus_tag="VFW59_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5364102.1"
                     /translation="MFAKFFIDRPVFAWVIALIILLAGALALRGMPVTQYPAVAPPAL
                     SVSITYPGASAQVIEETAVALLEQEMNGIENLLYMESSSNLGSGSITLTFEAGTNLDL
                     AAVETQNRIKRAEARLPEEVRRLGITVAKSARNYVMFIALFSPNKSMDDVALGSYAAA
                     NVLDSIRRVPGVGEAMLFGTEYSMRLWLQPEKLQSYGLSPADVSRAVRAQNSQLATGE
                     LGQLPAVAGQQTNAVIVTRSRLSTPEEFGEIIIRANPDGSALRVKDIARVELGAQDYS
                     RNARLDGQPTAAIAIRLTPGANALETVGAIKSRMGELAKFFPEGIDWAVPYDTSKFID
                     ISIKEVLKSLAEALVLVVLVMYLFLGNLRATLIPTIVIPVALVGAMVGLYLLGYSINV
                     LTLFAMVLAIGLVVDDAIVVVENVERIMTTEGLSPRDATRKAMDQIVGAIIAITLVLS
                     AVFIPMAFFAGSTGVIYRQFAVTLVLTMAFSALMALTLTPALCATLLKHTVAQKREGW
                     FTRFFARATRGYSTLVTGVVTKAGRYLLIYAVILLAMGWLFAKLPNSFLPEEDQGYFI
                     NVIQLPAGATQERTLAVLSQVEQYYLGLPEVDHVVGVAGFSFFGRGQNAAIAFVRLKG
                     WDERPGADSSAPALARKANMMLFRIKQAMIFAINPPPIPELSAAGGFDFRLQDRGGVG
                     YEKLLEARGMALGLAMKEPALAGVRPEGQEAGPQLFLDIDRIKAETLGVSITDLNETL
                     QSMLGTAYINDFVRQGRVLRVQMQAEADTRRTPEDLLNVTVRNNKGGMVPLGELATAR
                     WVVGSPKLDRYNGLPAMKISGSAAPGHSTGEAMAAMERVAEKLPPGLGFEWSGASREE
                     MLSGAQAPLLFGISMIVVFLCLAALYESWSIPFAVMLVVPLGVFGAVLAVTLRELPND
                     VYFKVGLIAIIGLSAKNAILIIEFARKLQDDGMGLIEATLEACRLRFRPILMTSIAFI
                     AGVLPLAISTGAGAQSRHAIGTGVMGGMIAATVLAVFLVPVFFVVVRRFFPGHARKQA
                     EHEETHHE"
     gene            4555..6003
                     /locus_tag="VFW59_07520"
     CDS             4555..6003
                     /locus_tag="VFW59_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX5364103.1"
                     /translation="MNSKLALAALTLALAGCSLAPAYQRPAAPLPSAWPDQARALPGE
                     RLAHADGWQDYFSDPRLQALIAASLENNRDLRIATARIAEARAQYGIQQADRLPNFNL
                     SANRNASLTPAGASAFSPNALHSQRYDVSVGLTAFELDFWGRVASLNAAAKASYLATE
                     SAQRAFRLSLISDVANAYLSVLELRERSELAKSAAEARAESRMLASRRRELGASGDLD
                     YLQVEAAYQAALAEQANLARQLAAAGNLLDLLVGRGVTGMNDLPAGRPLAAQDIGTAR
                     IADLPAEALLRRPDVVAAEQRLIASNANIGAARAAFLPRIALTGSAGTASRTLTGLFD
                     AGSGAWSFQPSITLPLFDAGRNSANVDLAEARKVIAVAEYEKTLQQAFREVSDLLSAR
                     ARLAEQLAAQQANATAQAQLLTLVDARYQAGIANRLELLDAQSASFTSRQNELQLRRT
                     LLTATAQLYKALARGADDLSETETAPRGVATK"
     gene            6000..6647
                     /locus_tag="VFW59_07525"
     CDS             6000..6647
                     /locus_tag="VFW59_07525"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HEX5364104.1"
                     /translation="MNVAVECKSVSEQTRERLVTAAREVFSECGFQGATVREICRRAG
                     ANIAAVSYHFGSKDGLLAEALHFESLRLLQETNAQSDACPEIRLGLFIRDFMHMLLDE
                     HNASSQCRIMARELADPTPGLDKIVREAIAPLHGFLARLTREIVGDKIGEAALHRCVH
                     SIMGQCLLYHHSHPVLQRLHPELRFDSQEIDAIARHIADFSLNGLRALGKQDSRP"
     gene            complement(6644..7096)
                     /locus_tag="VFW59_07530"
     CDS             complement(6644..7096)
                     /locus_tag="VFW59_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX5364105.1"
                     /translation="MKQNNFLLMLAISIAVAGCASGKGGDVYTREEARQVQTVQTGVV
                     EGARPVKIEGTKSMIGTGAGAVVGGVAGSTVGQGKDAQIGAVIGAVIGGIAGSAIEEG
                     TTREDAMEITIKLDSGRMISVVQGGKEEFKPGDKVRVLQINGETRVAR"
     gene            7342..8931
                     /locus_tag="VFW59_07535"
     CDS             7342..8931
                     /locus_tag="VFW59_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5364106.1"
                     /translation="MTDPTQSPSSITPIQRRVVLAACFGTFLEWYDFLTFASLATYFS
                     VLFFPSDNPVAALLASLATFGVGMLVRPLGAAVFGSLGDKYGRRPIFITTIFLMGMTT
                     FAVGLLPTYAQVGMLAPVLLLVLRLLQGFAVGGEIGGAAVYLTEHAPTNRRGFYTSVL
                     QLMGPLGIMASMLQVVALQYWLSPAEFSEWGWRVPFLLSILLLAISMKSRMSMIESPI
                     YLEMQESNTLSKAPLRECLRDRRTLGRMALLFFCISAGGSLLFFSSQVYTNVYLKSVV
                     KLDARLAGTLVMFSTLALFPLTVFCGWLSDRIGRRPIMLAGLLLGALAIFPVFLGLLH
                     YGNPALAQFNRNVTVQLSGEGCHYNPFVSPRNDCERNQEWLTKQGVIYQLNEADGTPV
                     QVAVQDQLPVAAFQPDELKRTLQAADWPEQADAQQTNRYAIVGLLLVLVLALAMITGP
                     QTAVLAELFPARTRYTAAALPHNLSAGWIGGMSPFMVTLIGTRMGDVSLGITYPVALL
                     SLAFVVGLLFLPETKNVPLEH"
     gene            8948..9976
                     /gene="pyrC"
                     /locus_tag="VFW59_07540"
     CDS             8948..9976
                     /gene="pyrC"
                     /locus_tag="VFW59_07540"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5364107.1"
                     /translation="MELKITRPDDWHLHLRDGALMQSVLPDTARQFARAIVMPNLRPP
                     VTTTVQAQEYRARILAALPQGMKFEPLMTLYLTDNTSASEIKKAVSSGIVQAVKLYPA
                     GATTNSDAGVTDIRKTYAALEEMQRSGMPLLVHGEVTENSIDIFDREAVFIERVMQPL
                     LKDMPELRVVFEHITTQDAAQFVTNAPDNIAATLTPQHLLYNRNAMLVGGIRPHFYCL
                     PILKRETHREALVKAATSGNKKFFLGTDSAPHAQHTKENACGCAGCYSAHSAIELYAE
                     AFEAAGALDKLEGFASFHGADFYRLPRNTDTITLRKEEWQVPASVGFGEHQLVPLRAN
                     ETMKWKLL"
     gene            9986..10531
                     /locus_tag="VFW59_07545"
     CDS             9986..10531
                     /locus_tag="VFW59_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5364108.1"
                     /translation="MSVMPYLNASPVIGERVYLHPSCQVIGDVTIGDDSSVWCNTVLR
                     GDVNRIVIGRGTNVQDLTMGHVSHKTTEKPEGSPLVIGDYVTVGHSVILHGCTIGNEC
                     LIGMGSIVMDDVVIPDRVMVGAGSLISPGKVLESGMLYMGRPAKPVRALTADETAYLR
                     YSAEHYMRLKDNYLKDLKLSA"
     gene            complement(10663..11091)
                     /locus_tag="VFW59_07550"
     CDS             complement(10663..11091)
                     /locus_tag="VFW59_07550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX5364109.1"
                     /translation="MAMTVHVDVVSAEESIFSGLAEFVIIPGEDGELGIYPRHSALMT
                     RIKPGSVRVKRPDQEQEELIYVSGGMLEVQPGVVTVLADTAIRGGDLDEVRALEAKQA
                     AEEAMKNRSSDIDYAQAQAELLEAMAQLHVIQQLRKQGRH"
     gene            complement(11107..12528)
                     /gene="atpD"
                     /locus_tag="VFW59_07555"
     CDS             complement(11107..12528)
                     /gene="atpD"
                     /locus_tag="VFW59_07555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5364110.1"
                     /translation="MSQGKIVQCIGAVVDIEFPRDQMPKVYEALQLADAGTSTVEAGL
                     TFEVQQQLGDGVVRTIAMGSSDGLRRGMLVNASGNGISVPVGTGTLGRIMDVLGRPID
                     EAGEIKCTEKREIHQKAPKFDELSPSVDLLETGIKVIDLVCPFAKGGKVGLFGGAGVG
                     KTVNMLELINNIAKQHSGLSVFAGVGERTREGNDFYHEMSEAGVIDTADFTKSKVAMV
                     FGQMNEPPGNRLRVALSGLTMAEYFRDEGRDILFFVDNIYRYTLAGTEVSALLGRMPS
                     AVGYQPTLAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFLHLDSTVVL
                     SRDIASLGIYPAVDPLDSTSRQLDPLVVGEEHYNVARGVQQTLQRYKELRDIIAILGM
                     DELAPEDKLAVARARKIQRFLSQPFHVAEVFTGAPGKYVTLKDTIKGFKGIVEGEYDH
                     LPEQAFYMVGSIEEAVEKAKTLQ"
     gene            complement(12556..13419)
                     /gene="atpG"
                     /locus_tag="VFW59_07560"
     CDS             complement(12556..13419)
                     /gene="atpG"
                     /locus_tag="VFW59_07560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX5364111.1"
                     /translation="MPGSKEIRTKIKSVENTRKITRAMEMVAAAKMRKAQERMRAARP
                     YAEKIRNVAAHLSRANPEYKHAFLVKRESIKNVGLIVVTSDKGLCGGLNTNVLRLAVS
                     QMKTWEGEGKGLTVCPIGNKGFGFMNRVGAKVGSHLTGLGDTPHIEKLIGAVKVLLDA
                     YVAGEIDAIYLSYNHFVNTMKQEPVVEQLLPLSGEQLGTAEGSWDYIYEPDAKVVIDE
                     LLVRYIESLVYQAVVENMASEQSARMVAMKAASDNAKSVIGELKLVYNKARQAAITKE
                     ISEIVGGAAAV"
     gene            complement(13433..14974)
                     /gene="atpA"
                     /locus_tag="VFW59_07565"
     CDS             complement(13433..14974)
                     /gene="atpA"
                     /locus_tag="VFW59_07565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5364112.1"
                     /translation="MKLNPSEISNLIRSRIDNFEALTQARTEGTVVSVTDGIVRVHGL
                     SDVMQGEMLEFPGNTFGLALNLERDSVGAVVLGDYEHITEGDTVKCTGRILEVPVGPE
                     LRGRVVNALGQPIDGKGPINAKMTDKIEKLAPGVISRKSVDQPVQTGLKSIDSMVPVG
                     RGQRELIIGDRQTGKTAVAVDAIINQKGQNMTCIYVAIGQKASTVANVVRKLEEHGAM
                     EYTIVVAATASDSAAMQFLAPYAGCTMGEYFRDRGEDALIVYDDLTKQAWAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNADYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESDLFNAGIRPAINAGVSVSRVGGAAQTKVIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLERGRLVTELMKQLQYCPLSVAEMAVTLFT
                     VNKGYFDDVPVAKALAFESALHANLKSNNKSLMDAIESTKDLSADNEKLLVAAIETFK
                     ATAVY"
     gene            complement(15005..15538)
                     /locus_tag="VFW59_07570"
     CDS             complement(15005..15538)
                     /locus_tag="VFW59_07570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX5364113.1"
                     /translation="MSEAITTARPYAQAAFDEAQKLSDLKGWSDVLKSLAEAVLNPEL
                     RSVISSPRVAKAQLAGLLSDVLGGQLKPQQLNLIKVLAENQRLLVSPEISAIFEMLKA
                     EAEKTVNVVVESAFELSAAQQEKIVVSLKKRMAREVKLVCNVNKELLGGVVIRAGDKV
                     IDGSARTRLGEMANALA"
     gene            complement(15542..16012)
                     /locus_tag="VFW59_07575"
     CDS             complement(15542..16012)
                     /locus_tag="VFW59_07575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5364114.1"
                     /translation="MNINLTLFAQAMTFAILIWFTVKFVWPPLLAAIESRQKTIADGL
                     ADAERAKHDLELAAKRSADILREAKEKAGDIVASGEKRASEIVEQAKAQAKVEGDRIV
                     AGAKAEVDQEVFRAKEQLRTQVSAIALAGAGKILGREIDAKAHNDLLEKLAAEL"
     gene            complement(16058..16333)
                     /gene="atpE"
                     /locus_tag="VFW59_07580"
     CDS             complement(16058..16333)
                     /gene="atpE"
                     /locus_tag="VFW59_07580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5364115.1"
                     /translation="MENTVQFLAMVQAYTGIGIGLIIGLGAAGACIGIGIMCASFLDG
                     AARQPELIPTLQGKVFLLLGLIDASFIIGVGLAMMFAFANPLLAVIK"
     gene            complement(16389..17198)
                     /gene="atpB"
                     /locus_tag="VFW59_07585"
     CDS             complement(16389..17198)
                     /gene="atpB"
                     /locus_tag="VFW59_07585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5364116.1"
                     /translation="MSTEHELTPSSYIEHHLSFNVKQVAEGGFWTVNFDTLVVSGLLG
                     LITFGLLWWVVRGATAGVPTKRQAFVELLVEFVDDQVKGIFHGDRHKFVAPLALTVFV
                     WVLMMNAMDFLPIDIMAWIYHNVFGLEKFRAVPTSDINATFALALSVWFLMIFFSIKV
                     KGLGGWIHELFCAPFGSNPLVWPANLLFNLIEYVSKPLSHSLRLFGNMYAGEIIFLLL
                     GMWAATGLAGTIFGALLGAGWAIFHILIVVLQAFIFMMLTVVYIAMAHESH"
     gene            complement(17207..17626)
                     /locus_tag="VFW59_07590"
     CDS             complement(17207..17626)
                     /locus_tag="VFW59_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX5364117.1"
                     /translation="MNKNSQGLTMDKVINKKISRAFSKAARWQIIITALISIVSLLLA
                     GVNAAISAIAGGASVLVGAYVGMVLARRPSGGTPGGVLITLLKAEAVKVLVIAVLLLV
                     TFKYYQGLVPLALIGGLAGSALASGAGLRTVNNENNE"
BASE COUNT         3674 a         5581 c         4974 g         3644 t
ORIGIN      
        1 aaacacctat gagttcgcag gccgcatctt gccgcaagtg caacctcaca tcaacccgct
       61 tcgagagcca aaccgggtac gaccggctac cgcaggttga ttttcaaaca tgcgtttgat
      121 atgctcgtcc cgcctctcca ccgggtatcg cctttcgctc tgacgatgtc gcagacatga
      181 ctggcatgat gccgggcaat gacaagacgg cgcagcgaac tgccggcaac gacaccatcg
      241 attcctcagg attattggaa accaacatga cgaatatcaa ccggcctttt gcgctcctgt
      301 taatgctttc cgccttgctg tcagcctgca acgacaaaca ggcgacgccc cccggcggcc
      361 cgggagccgg catgccgcca cccgaagtag aggtgattga ggtcgatgcc ggaaacgcga
      421 cactgactcg cgacctgacg gggagattgc aggcctaccg cacggcacag gtgcgcgctc
      481 gcgtcgaggg ggtgattgag aagcgcctgt tcaaggaagg cagcgaggta aaggccggga
      541 catcgctgta tcgcatcgat gcgcgcacct atcgcgctac ggcagaagcg gcacgagccg
      601 atctgtcgat cgcccgccag accgtcaacc gttatcgctc gctgctggac atcaaggcgg
      661 tcagccagca ggaatatgag ctggcgatgg gcaagttcaa gcaggccgag gccgcgctga
      721 cccgggcaca ggaagacctg gaaaatgccg aggttcccgc acccatctcc ggacacatag
      781 gccgcgcagc ggtgaccgaa ggcgcactgg tcggcaaggg cgaagccacg ctgctggcga
      841 ctatcgaaca gctcgatccg gtctatgcca acttcaccca gtccagcaac gatttgctgc
      901 gtctgagaca agcgattgcc tctggccagc tcaaacgccc cgattctgcc gaagtcgagc
      961 tgctgcttga agacggcagc gtctacccgc tgaagggacg gactttcttt accgacatgg
     1021 cggtcgatcc gagcaccggc tcggtggcat tgcgcgccga gttcgccaac ccccagcacg
     1081 cgctgttacc gggcatgttc gtgcgcgtcc gcttccccga ggcggtggcg aacaacgtca
     1141 tacgcgtacc gcaacgcgcc gtgctcgccg ggccacaggg tcaattcgtg atggtggtcg
     1201 gcgcggagga caaggtcaga ccgcaaccgg tgaagacggc cggcatggta gccggcgact
     1261 tcatcatcac ctccggactg accggaggcg aacaggtcat cgtgaatggc ttgcagaaag
     1321 cgaggccggg caccgtggta aagccggtac cgctgggcgg cgcaacatcg ccggccgcct
     1381 cggctccggc agctatcccg gccgcctcat cgaccgctgc cactggcaag caataaggac
     1441 gcgccatgtt tgccaagttc tttatcgatc gtccggtctt cgcctgggtc attgccctga
     1501 tcatcctgtt ggccggcgcc ttggcgctgc gcggcatgcc cgtcacgcaa tacccggccg
     1561 tcgcgccccc cgctttgtcg gtctccatca cctatccggg cgcctcggcg caagtgatcg
     1621 aggaaacggc ggtcgcgctg ctcgagcagg aaatgaacgg catcgagaat ctgctgtaca
     1681 tggagtcgtc gtccaatctg gggagcggca gcatcaccct gacctttgag gcgggcacca
     1741 atctcgatct ggccgccgta gaaacacaga accgcatcaa gcgcgccgag gcgcgtctgc
     1801 ccgaagaagt gcggcgtctg ggcatcaccg tggcgaaatc ggcgcgcaac tacgtgatgt
     1861 tcatcgcgct gttttcaccc aacaagagca tggatgacgt ggcgctgggc agttacgctg
     1921 ccgccaacgt gctcgacagc atccgccgcg tacccggcgt gggcgaggcg atgctgttcg
     1981 gcaccgaata ttccatgcgc ctgtggctgc aaccggagaa gctgcaaagc tacggcctgt
     2041 ccccggcgga cgtgtcgcgc gcggtgcgcg cccagaattc ccagctcgcc accggcgaac
     2101 tgggccagct acccgcagtg gcgggccagc agaccaatgc cgtgatcgtc acgcgcagcc
     2161 gcctctccac accggaggaa ttcggcgaaa tcatcattcg cgccaacccg gatggctcgg
     2221 ctctgcgcgt gaaggatatc gcgcgggtgg agcttggcgc acaggactac agccgcaacg
     2281 cccgtctcga cgggcaaccc accgcagcca tcgcgatccg cctgaccccc ggtgccaacg
     2341 cgctggagac ggtcggcgcc atcaagtcgc ggatgggcga actggccaag ttcttccctg
     2401 aaggaatcga ttgggccgtg ccttacgaca cctcgaaatt catcgacatc tcgatcaagg
     2461 aagtactcaa gagtctggcc gaagctctgg tgctggtggt gctggtgatg tatctgttcc
     2521 tcggcaatct gcgcgcgacg ctgattccca ccatcgtgat cccggtcgca ttggtcggtg
     2581 cgatggtggg cctgtatctg ctgggctatt ccatcaatgt gctgaccctg ttcgcgatgg
     2641 tgctggccat cggcctcgtg gtggatgacg ctatcgtggt cgtggaaaac gtcgagcgca
     2701 tcatgacgac cgaaggcctg tcgccgcgcg acgcgacgcg caaggcgatg gatcagatcg
     2761 tcggcgcgat catcgcgatc acgctggtgc tgtcggcggt attcatcccg atggcattct
     2821 tcgccggctc gaccggcgtc atctatcgcc agttcgccgt cacgctggtg ttgaccatgg
     2881 cattctcggc gctgatggcc ctgacgctga ccccggcgct gtgcgcgacc ctgttgaagc
     2941 atacggtcgc ccagaagcgc gaaggctggt tcacccggtt ctttgcccgc gcgacccgag
     3001 gctatagcac cctggtgacc ggagtcgtca ccaaggcggg gcgttacctg ctgatctatg
     3061 cggtcatcct gctggcgatg ggctggctgt tcgccaagtt gccaaacagc ttcctgccgg
     3121 aagaagacca gggctatttc atcaacgtca tccagctacc ggctggcgca acacaggaac
     3181 gcacgctggc cgtgctgtcg caggtcgagc aatactacct tggcctgccg gaagtcgatc
     3241 acgtggtggg cgtagccgga ttctccttct tcggccgcgg tcagaatgcg gcgatcgcct
     3301 tcgtgcgcct caagggctgg gacgaacgcc ccggtgcgga cagcagcgcc cccgcgctgg
     3361 cacgcaaggc gaacatgatg ctgttccgca tcaagcaggc gatgatcttc gccatcaacc
     3421 cgccccccat ccccgaactg tcggcggctg gcggattcga cttccgcttg caggatcgcg
     3481 gcggcgtagg ctacgaaaaa ctgctggaag cgcgcggcat ggcgctgggc ctggcgatga
     3541 aagaaccggc attggccggt gtgcgtccgg aagggcagga ggccgggccg cagctgttcc
     3601 tcgacatcga ccgcatcaag gccgaaacgc tgggcgtctc gatcaccgac ctcaacgaga
     3661 ccttgcagtc catgctgggc acggcttata tcaacgactt cgtgcgtcag ggcagggtac
     3721 tgcgtgtgca gatgcaggcc gaggccgata cccgccgtac accggaggat cttctgaatg
     3781 tcaccgtacg caacaacaag ggcggcatgg tgccgctggg cgaattggca acggcacgct
     3841 gggtggtcgg ttcgcccaag ctggatcgct ataacggcct gcccgcaatg aagatttccg
     3901 gctcggcggc tcccggccac agcaccggcg aggcgatggc ggcgatggaa cgggttgctg
     3961 aaaaattgcc gccgggactc ggcttcgaat ggtctggcgc atcgcgtgaa gagatgttgt
     4021 cgggtgccca ggcgccgttg ctgttcggca tctccatgat cgtggtgttc ctctgtctgg
     4081 cggcactgta tgagagctgg tctatcccgt ttgcagtgat gctggtggtg ccgctcggcg
     4141 tattcggagc ggtactggcc gtcaccctgc gcgaattgcc caacgacgtg tacttcaagg
     4201 tgggcctgat cgctatcatc ggcctgtcag ccaagaacgc catcctgatc atcgagtttg
     4261 cacgcaagct gcaggacgac ggcatgggtc tgatcgaagc gacgcttgaa gcttgccgtt
     4321 tgcggttccg ccccatcctg atgacctcca tcgccttcat cgccggagtg ctgccgctgg
     4381 ccatctccac cggagctggc gcacaaagcc gccatgccat cggcaccggc gtgatgggcg
     4441 gcatgatcgc agccacggtg ctggcggtgt ttctggtgcc ggtattcttc gtggttgtgc
     4501 gccgtttctt ccccggccat gcacgcaaac aggccgagca tgaggagacc catcatgaat
     4561 agcaagcttg cactggcggc attgacgctg gcactggccg gttgttcgct ggccccggcc
     4621 taccagcgcc ctgccgcacc gctcccctcc gcctggccgg accaggccag agcattgccg
     4681 ggcgaacgtc tggcacatgc cgacggctgg caggattact tttccgatcc ccgcctgcaa
     4741 gcgctgatcg ccgcatcgct ggaaaacaac cgcgacctgc gcatcgccac cgcccgcatc
     4801 gccgaagccc gcgcacaata tggcatccag caggccgatc gcctgcccaa tttcaatctg
     4861 agtgccaacc gcaatgcttc gctgaccccg gctggcgcgt ccgccttcag ccccaacgcc
     4921 ctgcacagcc agcgttatga cgtcagtgtc gggctgactg cgttcgaact ggatttctgg
     4981 ggccgcgttg ccagcctgaa tgcggcggcc aaggcgagtt atctcgccac cgaatcggcg
     5041 caacgcgcct tccgcctgtc gctgatatcc gacgtggcga acgcttacct gtccgtgctg
     5101 gagttgcgcg aacgcagcga attggcgaag agcgccgccg aggcgcgtgc cgaaagccgg
     5161 atgctggctt cccgtcgccg tgaactgggt gcatccggcg atctggatta cctgcaggtg
     5221 gaggccgcct atcaggccgc gctcgccgag caggccaatc tggcacgcca actggccgca
     5281 gccggaaatc tgctcgacct gctggtcggt cggggagtga ccggcatgaa cgacctgccc
     5341 gccggtcgcc cgcttgccgc acaggacatc ggcactgccc ggatcgccga tctgcccgcc
     5401 gaagcgttgc tgcgtcgtcc cgatgtggtt gccgccgagc agcgcctgat cgcttcgaac
     5461 gccaatatcg gcgcagcccg tgccgccttt ttgccgcgca tcgcgctgac cggcagtgcc
     5521 ggtacggcta gccgcaccct gaccggactg ttcgatgcgg gcagcggtgc atggagcttc
     5581 cagcccagca tcactctgcc gttgttcgac gccggacgca actcggccaa cgtagatctc
     5641 gccgaagcac gcaaggtgat cgccgtagcc gaatacgaga agactttgca acaggcattc
     5701 cgcgaagtgt ccgacctgct gagcgcacgc gcccgactcg ccgaacaatt ggccgcccag
     5761 caggcgaatg caacggcaca ggcgcaactg ctgacgctgg tcgatgcgcg ctatcaggcc
     5821 ggtatcgcca accgtctgga actgctggac gcacagagcg cctcctttac cagccggcaa
     5881 aatgagctgc agttgcgccg cacgctgctg acggccaccg cgcaactcta caaggcgctg
     5941 gcgcgcgggg ccgacgacct gtctgaaacc gagactgccc cacggggggt cgctaccaaa
     6001 tgaatgttgc cgtcgaatgc aaaagcgtca gcgaacagac ccgcgaacgt ctggtgacgg
     6061 cagcgcgcga agtgttttcg gaatgcggtt ttcagggagc gaccgtgcgg gagatctgcc
     6121 gccgtgccgg ggccaacatc gcggcggtga gctaccattt cggcagcaag gacggcctgc
     6181 tggccgaggc gctgcacttc gagtcgttac gactgctgca ggaaaccaat gcccagagcg
     6241 atgcctgtcc ggaaatacgg ctgggcctgt tcatccgcga cttcatgcac atgctgctgg
     6301 acgagcataa cgcctcgtcg caatgccgca tcatggcacg cgaactggcc gaccccactc
     6361 ccgggctgga caagatcgtg cgcgaagcca ttgcaccgct gcacggcttt cttgccagac
     6421 tgacacgcga gatcgtcggt gacaaaatcg gcgaagcggc gctgcatcgc tgcgtgcaca
     6481 gcatcatggg acaatgtctg ctctatcacc actcccatcc ggtgttgcaa cgcctgcacc
     6541 ccgaactgcg cttcgatagc caggagatcg atgccatcgc gcggcacatc gccgatttct
     6601 cgctgaacgg gctccgcgcc ttgggcaaac aagactccag accttagcgc gccacgcggg
     6661 tttcgccgtt gatttgaagc acccgcacct tgtcgccggg cttgaactct tctttgccgc
     6721 cctgtacgac cgagatcatt cgcccactgt cgagcttgat ggtgatttcc atggcatctt
     6781 cacgggtggt gccttcttct atcgccgagc ctgctatacc gccgataacc gctccgatga
     6841 cggcaccgat ctgggcgtcc ttgccctgtc cgacagtgct gccagcaacc ccgcccacta
     6901 ccgcgccggc acccgttccg atcattgatt tggtgccctc gatcttgacc ggacgggcgc
     6961 cttctactac gcccgtttga acggtttgca cctgacgcgc ctcctcgcgg gtataaacat
     7021 cccccccttt gccgctcgcg cagccagcaa ccgcaatgga tattgccaac atcagcagaa
     7081 aattattttg tttcatgacg gctatcccct aatcgttgtg tatgcctgaa tgatccgacg
     7141 gtgtaagtta caccaatcgc ttagttctgc tcaatagaat aaacagcagt gaacttcgat
     7201 gccatgattt catttccgtt ccggacggtt tctttatttt cgtatcactt tggattgaat
     7261 ccttctcaag acgtggcata ccccctgcga tctcgttcag tagcgtgtct cgccccatta
     7321 ccggcctgat agaatgccgg gatgactgac cctacccaat ccccctcctc cattacgcca
     7381 attcagcgcc gggtcgtcct cgccgcctgt ttcggcacct ttctggagtg gtatgacttt
     7441 ctgaccttcg cctcgctggc gacctatttc agcgtgctgt tcttcccttc cgacaatcct
     7501 gtcgcggcgt tgctggccag tctggcgact ttcggtgtcg gcatgctggt gcgcccgctg
     7561 ggcgcggcgg tgttcggttc gctgggggat aagtacggtc gccggcccat cttcatcacc
     7621 accatcttcc tgatgggtat gaccaccttt gcggtcggcc tgttgccgac ctatgcccag
     7681 gtcggcatgc tggcgcccgt cttgctgctg gtgttgcggc tgctgcaagg cttcgcggtg
     7741 ggcggcgaga tcggcggcgc agcggtatat ctgaccgagc atgcgcccac taaccggcgc
     7801 ggcttctata ccagcgtact gcaattgatg gggccgctgg gcatcatggc ttccatgctg
     7861 caagtggtcg cgctgcaata ctggttgagt cctgccgagt tcagcgagtg gggctggcgc
     7921 gtgccgttct tgctttccat cctgctgctc gccatctcca tgaagtcgcg catgagcatg
     7981 atcgaatcac ccatctatct ggagatgcaa gagagcaaca cgctgtccaa ggcaccgttg
     8041 cgcgaatgcc tgcgcgaccg tcgcacactg ggacgcatgg cactgctgtt cttctgcatc
     8101 tcggcgggcg gatcgctgct gttcttttct tcacaggtct ataccaacgt gtatctgaag
     8161 agcgtagtga aactcgatgc ccggctcgca ggcacgctgg tgatgttcag tacgctcgcg
     8221 ctgttcccgc tgaccgtttt ctgcggctgg ctctcggatc gcatcgggcg caggccgatt
     8281 atgctggcag gattgctgct cggtgcactg gccatcttcc cggtgtttct ggggctgctg
     8341 cattacggca accctgcgct ggcccaattc aaccgcaacg tcaccgtaca attatccggc
     8401 gagggctgtc attacaatcc cttcgtttcc ccgcgcaacg attgcgagcg caatcaggag
     8461 tggctcacca agcaaggcgt gatctatcaa ctgaatgaag ccgacggaac gccggtacag
     8521 gtcgcagtgc aagatcaact gccggttgcg gccttccagc cggatgaatt gaagcgtacg
     8581 ctacaggctg ccgactggcc cgaacaggcc gatgcacagc agaccaaccg ctacgcgatc
     8641 gtcggcctgt tgctggtgtt agtactcgca ctcgcgatga tcaccggccc gcaaaccgcc
     8701 gtactggccg agctgttccc cgcacgcacg cgctacaccg ccgccgcatt gccgcacaac
     8761 ctgagtgccg gctggatcgg cggcatgtcg cccttcatgg tcacgctgat cggcacgcgc
     8821 atgggggatg tgtcgctggg catcacttac ccggtcgcac tgctgagcct ggcattcgtc
     8881 gtcggtctgc tattcttgcc ggaaacgaaa aacgtccccc ttgaacatta aacctgtgag
     8941 caaaatcatg gaacttaaaa tcacccgacc cgacgattgg catctgcacc tgcgcgacgg
     9001 cgcgctgatg caatcggtat tgccggacac ggcgcgccag ttcgcccgcg cgatcgtaat
     9061 gcccaacctg cgtccgccgg tgactaccac ggttcaggca caggaatacc gcgcacgcat
     9121 cctcgccgct ttgccgcaag gcatgaagtt cgagccgctg atgacgctct acctcaccga
     9181 caacacctct gcctccgaaa tcaaaaaggc cgtgtccagc ggcatcgttc aagcagtcaa
     9241 gctgtatccg gcgggggcga ccactaactc cgatgcgggt gtgaccgaca tccgcaagac
     9301 ctatgccgcg ctggaagaga tgcagcgcag cggcatgccg ctgctggttc acggcgaagt
     9361 caccgaaaac agtatcgaca tcttcgaccg cgaagcggta ttcattgagc gcgtgatgca
     9421 accgttactc aaggacatgc ccgagttgcg cgtggtgttc gagcacatca ccacccagga
     9481 tgccgcacag tttgtaacga atgcgccgga caacatcgcg gcaacgctca cgccgcaaca
     9541 cctgctctac aaccgcaacg ccatgctggt cggcggcata cgtccgcact tctactgcct
     9601 gccgatactc aagcgcgaga cgcaccgcga ggccctggtc aaagccgcca ccagcggcaa
     9661 caagaagttt ttcctcggca ccgacagcgc gccccatgcg cagcacacca aggagaatgc
     9721 ctgcggctgc gccggatgtt acagtgcgca tagcgccatc gaactgtatg ccgaagcgtt
     9781 cgaggcggca ggcgcattag acaaactgga aggcttcgcc agtttccacg gcgcggattt
     9841 ttaccgcctg ccgcgcaata ccgacactat caccctgcgc aaggaagagt ggcaggtgcc
     9901 tgcgtcagtc ggatttggcg aacatcaact cgtgcctctc cgagccaatg aaaccatgaa
     9961 gtggaaactg ctgtaaggag atgccatgtc tgtgatgccc tatctgaatg cctcacccgt
    10021 catcggcgag cgcgtctatc tgcacccatc ctgccaagtc atcggcgatg tgaccatagg
    10081 cgatgacagt tcggtgtggt gcaacacggt gctgcgcggc gacgtgaacc gcatcgtgat
    10141 cgggcgcggc acgaacgtgc aggatctgac catggggcat gtatcgcaca agacgacgga
    10201 gaagccggaa ggctcaccgc tggtcatcgg cgactatgtg acggtgggac attcggtgat
    10261 cctgcatggc tgcactatcg gcaacgagtg cctgatcggc atgggttcca tcgtcatgga
    10321 cgatgtcgtg atccccgatc gggtgatggt cggtgcgggc agcctgatct cgccaggcaa
    10381 ggtgctggaa agcggcatgc tctacatggg tcgtccggcc aagcccgtgc gcgcgctgac
    10441 cgcagatgag accgcctatc tgcgttattc cgccgaacac tacatgcgcc tgaaagacaa
    10501 ttacctgaag gatttgaagc tgagcgcctg attgatcaag cagaaaccat cgatggtgaa
    10561 gcaagcgctc accgcatttg aagctctgca gccacgactg ctgcacccaa caaaaaagca
    10621 gccgaggctg cttttctgat tattaccgag caccgatcgg aatcagtgtc tgccctgctt
    10681 gcgcaattgc tgaatgacat gcaactgtgc catggcttcc agcaattcgg cttgcgcctg
    10741 agcgtagtcg atatcggaag aacgattctt catcgcttcc tcagccgcct gcttggcttc
    10801 cagagcacga acttcgtcca ggtcgccacc gcggatcgcg gtgtcggcca gtacggtgac
    10861 tacgccaggt tgcacttcca gcatgccgcc tgaaacgtag atcagttctt cctgctcctg
    10921 atccggacgc ttgacgcgaa cggaacccgg tttgatgcga gtcatcaggg cagagtgacg
    10981 tgggtagata cccaattcgc catcttcgcc cgggatgatt acgaactcag ccagaccgga
    11041 gaagatggac tcctccgcgc taactacgtc gacatgtacg gtcatggcca taatggatac
    11101 tcccgattat tgcagcgtct tggctttttc aactgcctct tcgatgctgc caaccatgta
    11161 gaaggcctgc tctggcagat gatcgtattc accttcaacg atacccttga agcccttgat
    11221 ggtgtccttc agggtgacgt acttgccggg cgcgccggtg aacacttcgg caacgtggaa
    11281 cggttgcgac aggaagcgct ggatcttacg ggcgcgagcc acggccagtt tgtcttccgg
    11341 tgccaattcg tccataccca gaatcgcgat aatgtcacgc agttccttgt aacgctgcag
    11401 agtctgctgc acgccacggg caacgttgta gtgctcttcg cccacgacca gcggatccag
    11461 ctgacgggag gtggagtcca gtggatcaac cgcagggtag atacccagcg aggcgatatc
    11521 acggctcaac acaacggtcg aatccaggtg caagaaggtc gttgcaggag acgggtcggt
    11581 caagtcatcc gctggcacgt aaacggcctg aatggaagtg atcgaaccga ccttggtgga
    11641 agtaatacgc tcttgcaggc ggcccatttc ttcagccagt gtaggctgat aacccacggc
    11701 ggaaggcata cgacccagca acgcggacac ttcggtaccg gccagtgtgt aacggtagat
    11761 gttgtccacg aagaacagga tgtcacggcc ttcatcacgg aagtattcag ccatggtcag
    11821 accggacagc gcaacgcgca gacggttgcc tggcggctcg ttcatctgac cgaacaccat
    11881 cgcaacttta gacttggtga agtctgcagt gtcgataacg cccgcttcgg acatttcgtg
    11941 gtagaagtcg ttaccttcgc gagtacgctc acccacgccg gcaaacaccg acagaccgct
    12001 gtgctgcttg gcgatgttgt tgatcagttc cagcatgtta acggtcttgc ccacaccggc
    12061 gccgccgaac agaccaacct taccgccttt ggcaaacggg caaaccaggt caataacctt
    12121 gataccggtt tccagcagat caaccgaagg agacagttcg tcaaacttgg gagctttctg
    12181 gtggatctcg cgcttttcgg tgcacttgat ctcgccggct tcatcgatcg ggcggcccaa
    12241 cacgtccatg atgcggccca atgtgccggt acccactggc acagagatgc cgttacccga
    12301 tgcattaacc agcatgccgc ggcgcaggcc gtcggaagaa cccatggcaa tggtacgcac
    12361 cacgccgtcg cccaactgct gctgcacctc gaaggtcagg ccagcttcaa cagtggaagt
    12421 acctgcgtcg gccaattgca aggcttcgta caccttgggc atttgatcac gcgggaattc
    12481 gatgtcaacc accgcgccga tacactgaac aatctttcct tgactcattt taagattccc
    12541 cgtctaaatt aattcttaaa ccgctgccgc accgccaaca atctccgaaa tctctttcgt
    12601 gatcgccgct tgacgcgctt tgttgtaaac cagcttgagt tcgccgatga cgctcttcgc
    12661 gttatcagaa gcggccttca tcgccaccat acgcgcactc tgctcggacg ccatattctc
    12721 tacgacagcc tgataaacca aggactcgat gtagcgcacc agcaactcgt cgatcaccac
    12781 cttggcatcc ggctcgtaga tgtagtccca gctgccttca gcggttccca gctgttcacc
    12841 ggacaacggc agcagttgct cgacaaccgg ctcctgcttc atggtgttga cgaagtggtt
    12901 atagctcagg tagatcgcat caatctcgcc ggccacgtaa gcatccagca gcaccttgac
    12961 agcgccgatc agcttctcga tatgcggagt gtcacccaaa ccggtcaaat gagaaccgac
    13021 cttggctccc acgcgattca tgaagccgaa tcccttgttg ccgattggac acaccgtgag
    13081 ccccttgccc tcgccttccc atgtcttcat ctggctgaca gccaaacgca gtacgttggt
    13141 attcaagccg ccgcacagac ccttgtcgga agtaacgacg atcaaaccga cgttcttgat
    13201 gctttcgcgc ttaaccagga atgcatgctt gtattccgga ttggcacgcg acaagtgggc
    13261 tgccacattg cggatctttt cggcataggg acgcgcggca cgcatgcgct cttgcgcttt
    13321 gcgcatcttc gctgctgcca ccatctccat tgcacgcgtg atcttgcgcg tattttcaac
    13381 gctcttgatc ttggtgcgga tttctttact accaggcatt tttcaccccg ccttagtaaa
    13441 cagccgttgc cttgaaggtc tcgatagccg caaccaacaa cttttcgttg tcggcggaca
    13501 ggtccttggt ggactcgatc gcatccatca gcgacttgtt gttcgacttc aggtttgcgt
    13561 gcaaggccga ctcaaatgcc agcgccttag caacaggcac atcatcgaag taacccttgt
    13621 tcacggtaaa cagcgtgact gccatttctg cgaccgacaa tgggcagtat tgcaactgct
    13681 tcatcaattc ggttaccaga cgaccgcgct ccagctgctt gcgagttgcc tcgtccagat
    13741 cggaagcgaa ctgcgcaaac gcagccagtt cacggtactg agccagagcc agacgcacac
    13801 cgccacccag cttcttgatg actttggtct gcgctgcacc acccacacga gacaccgata
    13861 caccggcgtt gatcgcagga cggataccgg cgttgaacag gtcgctctca aggaagatct
    13921 gaccgtcggt aatcgaaatc acgttggttg gaacgaatgc ggacacgtcg ccagcctgag
    13981 tttcaatgat aggcagcgcg gtcaaggaac cggtcttgcc cttcacttca cccttggtga
    14041 aagcttcgac atagtcggcg ttcacgcgag ctgcgcgctc cagcaaacgg gagtgcaggt
    14101 agaacacgtc gcctgggtac gcttcacgac caggtggacg acgcagcaac agggagatct
    14161 gacgataagc ccaggcctgc ttggtcaagt cgtcgtaaac gatcagcgca tcttcgccgc
    14221 gatcgcggaa atattcgccc atggtgcaac cggcgtacgg tgccaggaat tgcatcgctg
    14281 cggaatcgga agcggtagca gcaaccacga tggtgtattc catcgcgccg tgctcttcca
    14341 gcttgcgcac cacgttagcc acggtcgaag ccttctgacc gatcgccacg tagatacagg
    14401 tcatgttctg acccttttga ttgatgatcg cgtccacagc cacggcagtc ttgccggtct
    14461 gacggtcgcc gatgatcagt tcgcgctggc cgcggccgac tggaaccatg gagtcgatgg
    14521 acttcaggcc tgtttgcact ggctgatcaa ccgacttacg ggagataacg cctggcgcaa
    14581 gcttctcgat cttgtcggtc atcttggcgt tgatcgggcc cttgccgtca atcggctgac
    14641 ccagcgcatt cactacacga ccgcgcaact ctggaccaac cggcacttcc agaatacgac
    14701 cggtacactt gacagtgtcg ccttcggtaa tgtgctcgta atcacccagc accaccgcac
    14761 ccacggagtc gcgctccagg ttcagcgcga ggccgaaggt gttgcccggg aattccagca
    14821 tttcgccctg cataacatcg gacaagccgt gaacgcgaac gataccgtcg gttacgctga
    14881 ccaccgtacc ttcggtgcgc gcctgagtca gcgcttcgaa gttatcgatg cgactgcgaa
    14941 tcaaattgct aatttcggaa gggttgagct tcatctgggt ttcctcattc ttgataattc
    15001 tttatcaggc cagggcgttc gccatttcgc cgagtcgggt gcgcgcagaa ccgtcgatca
    15061 ccttatcccc ggcgcgaatc accacgccgc ccagcaattc tttgttaacg ttgcagacca
    15121 gcttaacttc gcgagccata cgcttcttca gcgatacaac gattttttcc tgttgagcag
    15181 ccgacaattc gaaagcagac tccaccacca cattgacggt tttttctgct tcagccttta
    15241 gcatttcaaa gatggccgaa atctccggcg acaccagcaa acgctggttc tcagccagaa
    15301 ccttgatcag gttcaattgc tgcggcttga gctgaccacc cagaacatcg cttagcagac
    15361 cggcaagctg cgctttagca acacgcgggc tactgatcac tgaacgaagt tccggattaa
    15421 ggacagcttc ggcaagagac ttcaacacat ccgaccagcc tttcaggtcg gacagctttt
    15481 gtgcttcgtc gaatgctgct tgggcataag gccgtgctgt ggtaatggct tcagacatgg
    15541 cttagagttc cgcggcgagt ttttcaagca aatcgttgtg cgccttggca tcgatctcgc
    15601 gacccagaat cttgcccgcc ccagccagcg caattgccga cacttgagtg cgcaactgct
    15661 ctttggcgcg gaaaacttct tgatcgactt cagctttagc tccggcaacg atacggtctc
    15721 cctcgacctt ggcttgcgcc ttggcctgtt caactatctc gctggcgcgc ttttcaccgc
    15781 tagccacgat atcgcccgcc ttttcttttg cttcacgcaa aatatcggca gagcgcttgg
    15841 ccgccaattc cagatcgtgc ttggcacgtt ccgcatcagc caagccatcc gcaatggtct
    15901 tttgacgcga ctcaatcgcc gccaacaaag gtggccagac aaatttgacg gtaaaccata
    15961 tcaaaatggc aaacgtcatt gcctgtgcaa acagtgtcag gttaatgttc atttcgattc
    16021 cttacgctgg atagtgccgc ctgcaatggt cgattaatta cttgataacc gcgagcaatg
    16081 ggttagcaaa tgcaaacatc attgccagac caacgccgat aatgaaggat gcgtcgatca
    16141 gacccagcag caggaacact ttgccttgca gtgtagggat cagctcaggt tgacgagcag
    16201 cgccatccag gaagcttgcg cacatgatac cgataccgat acaagcaccc gcagcgccca
    16261 gaccaatgat cagaccgatg ccgatgccgg tgtaagcctg aaccatcgcc aaaaattgta
    16321 ctgtgttttc catctttact tcctttcagt ttacaaggtt gattaaagtc aattactaca
    16381 aaaaatgttt agtggctttc gtgcgccatc gcgatgtata caaccgtcag catcataaag
    16441 ataaacgctt gcagcaccac gatcaggata tggaatatcg cccagcccgc gccgagcaac
    16501 gcaccaaaaa ttgttcctgc caaacccgtc gccgcccaca tgccgagcaa caggaagatg
    16561 atctcacccg catacatgtt gccgaacaga cgcaaggaat gagacaaagg cttggaaacg
    16621 tattcaatca gattgaacag caaattggcc ggccaaacca gaggattgct gccaaacgga
    16681 gcgcagaaca actcgtgaat ccagccaccc aaccccttga ccttgatgct gaagaaaatc
    16741 atcaggaacc atacggacag ggccagcgcg aaagtcgcat tgatatcgga ggtcggcacc
    16801 gcccggaatt tctccaggcc gaatacgttg tggtagatcc aagccatgat gtcgataggc
    16861 aggaagtcca ttgcattcat catcagcacc cagacgaaaa ccgtcagcgc cagcggggcc
    16921 acaaacttgt ggcggtcccc gtgaaagatg cccttgacct gatcgtcgac aaactcaacc
    16981 agcaactcaa caaatgcctg acgcttggta ggcacgccgg cggtggcgcc acgcaccacc
    17041 caccacaaca aaccgaaagt gatcagacca agcaaacccg acacgaccaa cgtgtcaaag
    17101 ttgaccgtcc agaatccgcc ctcggcgact tgtttgacgt tgaagcttag atgatgctcg
    17161 atgtaactgg atggtgtcag ttcgtgttca gtggacatcc cagaccctac tcgttattct
    17221 cattattcac ggttcgcaaa cccgcgccag atgccagcgc agaccctgcc aaccccccta
    17281 tcaaagccag cggaaccagc ccttgataat acttgaaggt aaccagcaat agcaccgcta
    17341 tcaccaacac cttaaccgcc tcagccttca gcaacgtaat cagcacaccg cccggcgttc
    17401 cgccactcgg cctgcgcgcc agcaccatcc cgacataagc acccacgaga acggacgcgc
    17461 ctcccgctat tgcggaaatc gccgcgttta cacccgccaa cagcagggaa acaatcgaga
    17521 ttagtgccgt gatgatgatt tgccaacgtg ccgctttgct gaatgcgcgg cttatttttt
    17581 tgtttattac tttatccatc gtcagccctt ggctattctt gttcatcaag ggcatcaaaa
    17641 gcccgcgcat tttctaccat ccccttgcgg gaagtcaagc ttaattacgc cctattctgc
    17701 gcgtaatcta gactcgcgtg cgcaacatgg ccatcaactc ttccagatgg tcattgctgg
    17761 aatactcaat gatcaatttg ccgccgcctt tgctgcctga tttgatttcc acgcgcgtac
    17821 caagataggc gctcatctct tcctgcaacc gctcagtatc ccggctgggc tgg
//
LOCUS       DASUIV010000209         9864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1352375, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000209 DASUIV010000000
VERSION     DASUIV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352375"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFW59_07595"
     CDS             <1..1197
                     /locus_tag="VFW59_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5364118.1"
                     /translation="AAYGCATCIGNAGPLREDIDKTITDNNLIAAAVLSGNRNFEARI
                     HPNLKANFLASPPLVVAYAIAGKMNINLDTEPLGMGRDGQPVFLKDIWPNSHEIQTVM
                     KYAADPAVYRKLYSDLTKDLPLWNAIEAPTGNQYQWDDSTYIACPPFFDAATPEVADI
                     KGAKALALLGDSVTTDHISPAGSFKPTTPAGKYLQGRGVAVKDFNSYGARRGNHEVMM
                     RGTFANVRIKNLMLPLNADGSRTEGGYTLYEGSQMAIYDAAMKHIADGTPTVIFAGEE
                     YGTGSSRDWAAKGTQLLGVKAVIAKSYERIHRSNLVGMGVLPLQFKGDDTAASLNLTG
                     DETFDLSGIAGDLKPQQDVTLIIHRRDGSQQSVSLLLRIDTPIEVDYYRNGGILPFVL
                     RELVGK"
     gene            1229..2065
                     /locus_tag="VFW59_07600"
     CDS             1229..2065
                     /locus_tag="VFW59_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5364119.1"
                     /translation="MTIDADLAIKELTSKGIKLPPQPKVLLELQQKFASDDYSMKQLA
                     AIIGSDPGITAMLFKAVRSPVFGGTKKLDDLEQVLMVIGVKQTYTLVQAVSLSSSLSN
                     KARKAFEVFWTRSGEVAQIAALIAAERVSICNIFPEQAYMAGIFHECGVPVLMQRFPN
                     YCDKLQLNKACCWPSLSEEDAQYNVDHSSIGYLVARHWKLPDFVCQAILYHHDMPREE
                     VGTVRTLVAILQLAIHFYHRIGRVDDPVWYKLRAEVLEELGLSVDGEQDYFELISEQF
                     LN"
     gene            2101..3051
                     /locus_tag="VFW59_07605"
     CDS             2101..3051
                     /locus_tag="VFW59_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364120.1"
                     /translation="MVFAHLFGQKQQAPDLLPAVDHAIASVEPLLKQINGYPDDYLAP
                     VSSALAYAHALATSLPGPLTVNRESYASDPLVHALFPSLESVTEALSSSLPLQTLLRQ
                     IPSDGELYALMGMRRQEKTVVGMQLSGQLVQHDVVQNMVYFTSHTIDNPASSELQARE
                     KIAMSFFDRLIAKVAQRIAQRKLGQQELLQEKDLLIAQLRSADEAARPALEGRLADLL
                     DRLQATVVTLELERYPEDFAAILLDPAQHLRLEQTPIKLDSMGIRLPDQDIAQGKIIV
                     FDDLIGYDRRNWTVTLVRCSDIPHEAFASKLERAYRTLTI"
     gene            complement(3106..3543)
                     /locus_tag="VFW59_07610"
     CDS             complement(3106..3543)
                     /locus_tag="VFW59_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX5364121.1"
                     /translation="MNRRELLAGGIALAGAALVGNAQAAAHDHGHHHGGAVSGALTLA
                     IGDCIQKGEVCLNHCIELLGQGEKEMAACAKTVNEVLAVCGALQQLANQNSQQLAKMA
                     SIAMEVCKQCEDECKKHADKHESCKACGESCAACYAECRKIAA"
     gene            complement(3569..4120)
                     /locus_tag="VFW59_07615"
     CDS             complement(3569..4120)
                     /locus_tag="VFW59_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5364122.1"
                     /translation="MSIRTVLRMGDVRLQQVAAPVRDFGSGDLRELLCDMRDTMKALD
                     GAGLAAPQIGVGLRVVIFGVLPDQQDPQNPRYPEAGSVPYTVLINPVITPLGDEREEG
                     WEGCLSLPGLRGLVPRFLHIRYQGCDEYGALIDRTVSGFHARVVQHECDHLDGILYPM
                     RMPDMSRFGYTAELFPGRESQEE"
     gene            complement(4210..5904)
                     /locus_tag="VFW59_07620"
     CDS             complement(4210..5904)
                     /locus_tag="VFW59_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364123.1"
                     /translation="MLTLGARLHSTRWLLLAMLVLLHCTLLFGVESPWARPLLLAHMG
                     LFLIWQPLWRGERELSPVALALIALAALAMTYWLNWWVVAFWLIGLFGLVGARVFSFR
                     DRWTRQLYLAVVAYLLAVLLLWVVPHLFAAQATIGVGRVIMSYMLPLWLVAMALLPGS
                     KEQPDEARAVDFIYSLLLFMLLALVVLGSLAFMTLAQLDYPDALLRTLFLIGLLLLAL
                     GGLWNPRFSPSLMRRTGFGGLRAMFSRYLLSVGTPFENWLRQLAEAAQREPDAPSYLR
                     RAIALLGDLPWLSGLSWQTSDDAGQIGEVTQHEVQVQEGGLRLTLYARHAHSPSILLH
                     VYLLAQMIGYFYQAKQREQNLRDITRLQAVYETGSRLTHDLKNMLQSLHSLTAIAQSQ
                     EERSKQLLQQQLPQLTRRIEQTLSKLHQPQQDDAAELALHVWWDGLRLRNQHQSVAWR
                     SPDVLPEIGIPAALFDCVLDNLLDNALKKRQAERDIAIAVELHSAPLCLRVCDSGSPI
                     AAQLSTNLLRNVVVSDNGLGIGLYQAARWAEQLGYRLTLSSNQPGAVCFELTEHKP"
     gene            6077..6706
                     /locus_tag="VFW59_07625"
     CDS             6077..6706
                     /locus_tag="VFW59_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5364124.1"
                     /translation="MKRHQSGFTLIEIAIVLVIIGLLLAGVMKGQELINSAKVKNLAA
                     DFKNVPVFIYGYQDKFKALPGDDSAAIAHVTATANGNGDGLIGGGWPAAGESLNFWQH
                     VRLAGLAAGPTDMAAADYIQKNAVGGDIGITSSADSPINNLKGSYIVCSKGIAGKFAK
                     QLDVTLDDSNTATGSLQVAVNTATTAAKANAEATAAIVDDTPYLVCMGF"
     gene            complement(6760..7506)
                     /locus_tag="VFW59_07630"
     CDS             complement(6760..7506)
                     /locus_tag="VFW59_07630"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEX5364125.1"
                     /translation="MLAIIGGRGLTELKNLKITHRQVLRTPYGEPSGAFMFGTLNQHE
                     VVFLARHGYGHTIPAHLVNYRANLWALREQGVREVVSVATVGGIRPDLTPGSIVLPDQ
                     IVDYTHGRDATFFDVRDSRYVNADFTLPYSAGLRRRILQSAAMTKQPCLDGGVYAATQ
                     GPRLDSAAEINRYERDGADMVGMTGMPETALAMELELNYATIAVVANYAAGRGDSKNG
                     INMEQVNATASAAMDKVRSILECVVSCSDD"
     gene            complement(7581..8135)
                     /locus_tag="VFW59_07635"
     CDS             complement(7581..8135)
                     /locus_tag="VFW59_07635"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEX5364126.1"
                     /translation="MNRSIQHANEILRNAELICPAEQVQAALQRVAQQIKDGLSGKRP
                     LVLSVMGGAVVFTGQLLPLLDFPLDFDYVHVSRYGDARQGGQMHWKVEPRENVRGRVV
                     LVLDDILDEGYTLAALKERVLELGASEFYSAVFADKQHGKAKPIHADFVGMELPDRFV
                     FGYGMDIEGAWRNLPAIYAVREQD"
     gene            complement(8138..8983)
                     /gene="galU"
                     /locus_tag="VFW59_07640"
     CDS             complement(8138..8983)
                     /gene="galU"
                     /locus_tag="VFW59_07640"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX5364127.1"
                     /translation="MGSRFLPATKASPKEMMPVVDKPLIQYAVEEAVAAGITDMIFIT
                     GRNKRAIEDHFDKAYELEAELEMRGKTELLRLVQEVIPEHVNCIFIRQSAPLGLGHAV
                     LCAQHVVNDEPFAVILADDLIDGEPPAVKQMVEVFWKHRCSVLGVQDVPRDQTKSYGI
                     VDATAFEPDIELVRGIVEKPRPEDAPSTLAVVGRYILTPRIFHHLEKVQKGAGGEIQL
                     TDGIAALMQEEQMLAYRFKGTRYDCGSKLGYLQATVALGLKHPELCAEFADYLISLKL
                     ERCCR"
     gene            complement(9088..>9864)
                     /locus_tag="VFW59_07645"
     CDS             complement(9088..>9864)
                     /locus_tag="VFW59_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5364128.1"
                     /translation="HIVREEGEADWRCSGGLFCPAQRKQALLHFAGRRAMDIEGLGDK
                     LVEQLVDGGIVHTPADLYHLGMSALVDLERMGEKSALNLLDAIEQSKHTTLARFIYAL
                     GIRNVGEATAKDLARHFGKLENLMQADEAALQQVPDVGPIVAQSLLAFFAEPHNLEVV
                     NALRACGVEWAEHEVQAVRESHLSGKTFVLTGTLSGMSRDQAKEKLEALGAKVSGSVS
                     KKTDYVVAGTEAGSKLEKALALGVAVLDEDKFMGLLGGEE"
BASE COUNT         2083 a         3275 c         2710 g         1796 t
ORIGIN      
        1 gcagcctacg gttgtgcgac ttgcatcggc aatgcgggcc cgttgcgcga agatatcgac
       61 aagaccatca ccgacaacaa cctgatcgcg gcggccgtac tgtccggcaa tcgcaacttt
      121 gaggcgcgca tccacccgaa cctgaaggcc aacttccttg cctcgcctcc tctggtggtg
      181 gcctacgcca tcgcgggcaa gatgaacatc aatctggaca ccgagccgct gggcatgggc
      241 cgcgacggac aaccggtatt cctgaaagac atctggccga acagccacga gattcagacg
      301 gtgatgaagt acgcggccga ccctgcggtg taccgcaagc tctacagcga cctgactaaa
      361 gacctgccgc tgtggaacgc catcgaagca ccaaccggca accagtacca gtgggacgac
      421 tccacctaca tcgcctgccc gccgttcttc gatgcggcca cacccgaagt tgccgacatc
      481 aagggggcga aggcgctcgc actgctgggc gactcggtca ccaccgacca catcagcccg
      541 gcgggcagct tcaagccgac cacccccgca ggcaagtacc tgcaagggcg tggcgtggcg
      601 gtgaaggatt tcaacagcta cggcgcgcga cgcggcaacc acgaggtgat gatgcgcggc
      661 accttcgcca acgtgcgcat caagaacctg atgctgccgc tcaatgcgga cggcagccgc
      721 accgaaggcg gctatacgct gtacgaaggc tcccagatgg cgatctacga cgccgcgatg
      781 aaacacatcg ccgacggcac cccgaccgtg atcttcgcgg gcgaagaata cggcaccggc
      841 tcttcccgcg actgggcggc caagggcacc cagttgctgg gcgtgaaggc tgtcatcgcg
      901 aagagctacg agcgcatcca ccgcagcaat ctggtgggca tgggcgtgct gccgttgcaa
      961 ttcaagggag atgacaccgc agcgtcactt aatctgaccg gtgacgagac cttcgatctg
     1021 agcggcatcg ccggcgacct gaaaccgcaa caggacgtga ccctgatcat tcaccgccgc
     1081 gacggcagcc agcagagcgt cagcctgctg ctgcgcatcg acaccccgat cgaggtggac
     1141 tactaccgca acggcggcat cctgcccttc gtgctgcgcg aactggtcgg caagtgattg
     1201 cccccctatt gaacggcgga aacccgacat gaccatcgac gcagatctgg cgatcaagga
     1261 actgaccagc aagggcatca agctgccacc gcaacccaag gtgctgctgg agctacagca
     1321 gaagttcgcc agcgacgact acagcatgaa acagctggcg gccatcatcg gcagcgaccc
     1381 cggcatcacc gcgatgctgt tcaaggcggt gcgctctccg gtattcggcg gcaccaagaa
     1441 gctcgacgac ctcgagcagg tgctgatggt gatcggggtc aaacagactt acacgctggt
     1501 gcaggcggtg tcgctgtcca gcagcctgtc gaataaggct cgcaaggcgt tcgaggtatt
     1561 ctggacccgt tccggcgaag tcgcccagat cgcggcactg atcgccgccg agcgcgtgtc
     1621 gatctgcaac atcttcccgg agcaggccta catggccggc atcttccacg aatgcggcgt
     1681 accggtgctg atgcagcgct ttccgaacta ctgcgacaaa ttgcagctga acaaagcctg
     1741 ctgctggccc agtctgagcg aggaagatgc gcaatataac gtcgaccaca gcagcatcgg
     1801 ctatctggtg gcgcgccact ggaagctgcc ggacttcgtg tgtcaggcga tcctgtatca
     1861 ccacgacatg ccgcgcgaag aagtcggcac ggtgcgcacg ctggtcgcca tcctgcaatt
     1921 ggcgattcac ttctaccatc gcatcggccg ggtcgatgat ccggtctggt ataaattgcg
     1981 cgccgaggtg ctggaggagc tgggcttgag cgtggacggc gagcaggatt acttcgaact
     2041 catcagcgag cagttcctga attaaaatca cccatcgctg gcaggcgtca caaaggaggc
     2101 atggtgttcg cacatttgtt cggtcagaag caacaagccc ccgatctgct gcctgccgtc
     2161 gaccatgcca tcgccagcgt ggagccgctg ctcaaacaga tcaatggcta tccggacgac
     2221 taccttgccc ccgtgtcttc cgcgctggcc tatgcccacg cgctggcgac cagtttgccg
     2281 gggccgctga ccgtcaaccg cgagtcctat gccagcgatc cgctggtgca cgcgctgttc
     2341 ccttccctcg agtctgtcac cgaagcgctg tccagcagcc tgcccttgca gaccctcctg
     2401 cgccagattc cctccgacgg tgaactgtat gccctgatgg gcatgcgtcg gcaggaaaaa
     2461 accgtggtcg gcatgcagct gtcggggcag ctggtacagc acgatgtcgt gcagaacatg
     2521 gtctatttca ccagccatac catagacaac ccggcatcca gcgagctgca ggccagagaa
     2581 aagatcgcca tgagtttctt cgaccgcttg atcgccaagg tggcgcaacg tatcgcgcaa
     2641 cgcaagctgg gccaacagga attgctacag gaaaaagatt tgctgatcgc gcaattgcgc
     2701 tctgccgacg aagcggcccg ccccgcactg gaaggtcggc tggcagacct gctggatcgt
     2761 ctgcaagcca cggtcgtcac gctggaactg gagcgttacc cggaagactt cgccgccatc
     2821 ctgctggatc cggcgcaaca cctgcggctg gaacagacgc cgatcaagct ggacagcatg
     2881 ggcatccgtc tgcctgacca ggatatcgcg cagggaaaaa tcatcgtatt cgacgatctg
     2941 atcggctacg accggcgcaa ctggaccgtt accctggtgc gttgcagcga catcccccac
     3001 gaagcctttg ccagcaagct ggaacgcgcc taccgcaccc tgacgatttg aaggagccgc
     3061 cacgaggcgg cgcccggacg gccaaatcct caagcctgat gccaatcagg ctgcgatctt
     3121 cctgcattcc gcataacagg cggcacagct ctcgccgcag gccttgcagg actcgtgttt
     3181 gtccgcgtgc ttcttgcact cgtcttcgca ctgcttgcac acctccatgg cgatggaggc
     3241 catcttcgcc agctgctgcg aattctggtt ggccagttgt tgcaacgcac cgcacaccgc
     3301 cagcacctcg ttcacggttt tggcacaggc cgccatttcc ttctcgccct gccccagcaa
     3361 ctcgatgcag tgattcaggc agacctcacc tttctggatg cagtcgccga tcgccagcgt
     3421 gagcgcaccg gaaaccgccc cgccatgatg atgaccatgg tcatgtgcag cagcctgcgc
     3481 gttgcccacc agcgcggcgc ccgccaatgc gataccgccc gccaacaatt ctcttctgtt
     3541 catgctcttc tccttggcca aaataaattt attcttcctg cgactcgcgc cccggaaaca
     3601 gctcggcagt gtagccgaaa cggctcatgt cgggcatccg catcggatag aggatgccat
     3661 ccagatggtc gcattcgtgc tgcacgacgc gtgcgtgaaa tccgctcact gtccggtcga
     3721 tcaacgcacc gtattcgtcg cagccctgat agcggatatg cagaaagcgc ggcaccagac
     3781 cccgcaaacc cggcagactc aggcaacctt cccagccctc ctcgcgctca tcgcccagcg
     3841 gcgtgatgac cggattgatc agcacggtgt aaggcacact ccccgcctcg ggataacgcg
     3901 gattctgcgg gtcttgctga tccggcagca caccgaatat cactacgcgc aagcccacac
     3961 cgatctgcgg cgcagccagc ccggcgccgt ccagcgcctt catggtgtcg cgcatgtcgc
     4021 acagcaactc gcgcaaatcc ccactgccaa aatcccgcac cggtgccgcc acctgctgca
     4081 ggcgcacatc ccccatgcgc aatacggttc tgatactcat atcggtttat cctgaaaaat
     4141 aaattatcca cgaaatacac gaacagaccc gggctggcaa cggattcgtt tcgcggatga
     4201 aataaggttt taaggtttat gttcagtcaa ttcaaaacac acggcacccg gctgattgct
     4261 gctcagcgtc aaccggtagc ccaactgctc cgcccagcgc gccgcctgat acaaccctat
     4321 ccccagaccg ttgtcggaga ccaccacatt gcgcaacaga ttggtggaca gctgcgccgc
     4381 gatcggcgag ccgctgtcgc agactctcag acacagcggt gccgagtgca actcgactgc
     4441 gatggcaata tcgcgctcgg cctggcgttt cttcagggca ttgtcgagca gattatcgag
     4501 tacgcaatcg aacaaggccg ccggaatccc gatctccggc aagacatcag ggctacgcca
     4561 tgccacggac tgatgctgat tgcgcagccg cagcccatcc caccagacat gcagcgcaag
     4621 ctcggcggca tcgtcctgct gcggctgatg cagcttgctc agcgtctgct cgatacgccg
     4681 cgtgagttgc gggagttgtt gttgcaacag ttgcttcgag cgttcctcct gactctgcgc
     4741 gatggcggtg agcgaatgca gcgactgcaa catgttcttc agatcatggg taagacgtga
     4801 gccggtttca taaacggcct gcaagcgagt gatgtcgcgc aggttctgtt cgcgctgctt
     4861 ggcctgatag aaatagccga tcatctgcgc cagcaaatag acatgcagca agatgctggg
     4921 gctgtgcgca tgccgggcgt acagtgtcag acgcagcccg ccctcctgca cctgcacctc
     4981 atgctgagta acctccccga tctgtcccgc atcatcgctc gtctgccagg aaagcccgga
     5041 cagccagggg agatcaccga gcaaggcgat ggcgcgacgc aaataggagg gcgcatcggg
     5101 ctcgcgctgc gccgcctcgg ccaactgcct gagccagttc tcgaacggcg ttccgacact
     5161 caacaggtag cgcgagaaca tcgcccgcag accgccgaaa ccggtccggc gcatcagcga
     5221 tgggctgaaa cgcggattcc acaatccgcc gagcgccagc agcagcaaac cgatcaggaa
     5281 cagagtgcgc aacagcgcat ccgggtagtc gagctgcgcg agcgtcatga aggccagact
     5341 gcccagcacc accagcgcca gcagcatgaa caacaacagg ctgtagatga agtcgaccgc
     5401 tcgcgcctca tcgggctgct ccttcgagcc gggcaataac gccatcgcca ccagccacag
     5461 cggcaacatg tagctcatga tcacgcggcc cacgccgatg gtcgcttgtg ccgcaaacag
     5521 atgcggcacc acccacagca acagcaccgc cagcagatag gcgaccacgg cgaggtagag
     5581 ctggcgcgtc cagcgatcac ggaaggagaa cacccgagcg ccgaccaatc cgaacagacc
     5641 gatcaaccag aaggcgacga cccaccagtt cagccagtag gtcatcgcca gcgcagccag
     5701 cgcgatcagc gccagcgcca ccggactcaa ctcgcgctcg cctcgccaca gcggctgcca
     5761 gatcaggaac agccccatgt gcgccagcaa cagggggcgc gcccacggac tctccacccc
     5821 gaacagcaac gtgcagtgca gcagcaccag catcgccagc agcagccagc gggtgctatg
     5881 cagacgcgcc cccagtgtca acatttcgtc atcaacctgt cgatatcctg tcaaaaaacc
     5941 accgtccacc actcctgacg ccatcgccac cctttgcaac aaggcggttc cgatgcagcc
     6001 gaatcacaat gacagcgcat tggcacgaca tttgcggtaa attaggcaca cactaaacaa
     6061 acgggagact accataatga aacgccatca atcaggcttc acactcatcg agatcgccat
     6121 cgtgctggtc atcatcggtc tgctgctcgc gggtgtcatg aaaggccagg agctgatcaa
     6181 cagcgcgaag gtcaagaacc tcgccgccga cttcaagaat gtccccgtgt tcatctatgg
     6241 ctatcaggac aagttcaagg ctctgccggg cgatgatagc gcagctatcg cccacgttac
     6301 ggcaaccgcc aacggcaacg gcgatggctt gatagggggt ggctggccag ctgccggcga
     6361 gtcgcttaac ttctggcaac atgttcgcct cgccggactc gcggccggac cgaccgacat
     6421 ggcggcagcc gattacatcc agaagaatgc cgtgggcgga gacatcggca tcaccagctc
     6481 agccgatagc ccgatcaaca acctgaaggg cagttatatc gtctgctcga aggggattgc
     6541 gggtaaattc gccaaacaac tggatgtcac gctggacgac agcaacaccg cgaccggctc
     6601 gctgcaagtg gcagtcaaca ccgcgacaac agccgccaag gccaatgcgg aagccaccgc
     6661 agccatcgtt gacgacaccc cttacctggt ctgcatggga ttctgatcga aatacccggc
     6721 gtgacattca agcccgcttc ggcgggcttt ttttgcgttc taatcgtcgc tgcagctcac
     6781 tacacactcc agaatgctgc gcaccttgtc catcgccgca ctggcggtgg cattgacctg
     6841 ttccatgttg atgccgttct tgctatcacc ccgccccgcc gcgtaattcg cgacgaccgc
     6901 gatggtggca tagttcagct ccagctccat cgccagcgca gtttcaggca tcccggtcat
     6961 acccaccatg tcggctccat cgcgctcgta gcgattgatc tcggcggcgc tgtccagccg
     7021 tggtccctgc gtcgcggcgt acacgccgcc atccagacaa ggctgtttcg tcatggcggc
     7081 actctgcagg atgcggcggc gcaaccctgc actgtagggc agggtgaagt cggcgttgac
     7141 atagcgcgag tcgcgcacat cgaagaaggt cgcatcgcgc ccgtgggtgt aatcgacgat
     7201 ctgatccggc aacacgatgc tacccggagt caggtctggg cggattccgc ccacggtcgc
     7261 caccgaaacg acctcgcgca cgccctgctc gcgcaaggcc cacaggttgg cgcgataatt
     7321 gaccagatgc gccggaatag tatgtccata gccatggcgc gccagaaaga ccacctcatg
     7381 ctgattcagc gtaccgaaca tgaatgcacc ggacggctcg ccgtagggcg tacgcaacac
     7441 ctgccgatgc gtgatcttca ggttcttcag ttcggtcagg ccgcgaccgc caataattgc
     7501 caacatttat ttctcctttg aggattgagg attgagaatt gagaaaaccc gatgcactgt
     7561 ttttacccga tcctcgctcc tcaatcctgc tctctgactg catatatcgc aggcaaatta
     7621 cgccatgcgc cttcgatatc catgccataa ccgaacacga agcgatccgg caattccatg
     7681 ccgacaaaat ccgcatgaat gggcttggcc ttaccatgct gcttgtcggc gaataccgca
     7741 ctgtaaaatt cgctggcgcc cagttccaga acacgctctt tcaatgccgc cagtgtgtaa
     7801 ccctcgtcga ggatatcgtc cagaaccagc accaccctac cgcgcacgtt ctcgcgaggc
     7861 tcgaccttcc agtgcatctg cccgccttgc cttgcatcgc cataacgcga cacatgcacg
     7921 taatcgaaat ccagcgggaa atcgagcaag ggcaacaact gaccggtaaa caccaccgca
     7981 ccgcccatca ccgaaagcac cagcggccgc ttcccactca ggccatcctt gatctgctgc
     8041 gcgacccgct gcaacgcggc ctgcacctgc tccgccggac agatcagctc ggcgttgcgc
     8101 agaatctcgt tcgcatgctg gatagaccta ttcatgctca ccggcagcac ctttccagct
     8161 tcaggctgat caggtagtcc gcaaactccg cgcacaattc gggatgcttc aaccccaatg
     8221 ccaccgtcgc ctgcaggtaa cccagtttgg agccgcagtc gtagcgcgtg cccttgaagc
     8281 gataggccag catctgctct tcctgcatca atgcggcgat gccgtcggtc agctggattt
     8341 cccctcccgc tcccttctgc accttttcca gatgatgaaa gatacgcggc gtcaggatgt
     8401 aacgcccgac caccgccagc gtggaaggag cgtcttccgg acggggcttc tcgacgatgc
     8461 cgcgcaccag ctcgatatcc ggctcgaacg cggtggcgtc gacgatgccg taacttttgg
     8521 tctgatcgcg cggcacatcc tgcaccccca gaacggaaca gcgatgcttc cagaacacct
     8581 ccaccatctg cttgacggca ggcggctcgc cgtcgatcag atcgtcggcg agaatgacgg
     8641 cgaacggttc atcattcact acatgctgtg cgcacaatac ggcatgcccc agccccaatg
     8701 gcgcagactg gcggatgaag atgcaattga catgctcggg aatgacctcc tgaaccaggc
     8761 gcagcaactc cgtcttgccg cgcatttcca gttcggcttc cagctcatag gccttatcga
     8821 aatgatcctc gatcgcgcgc ttgttgcgcc cggtgatgaa gatcatgtcg gtgataccgg
     8881 ccgcgaccgc ctcttccacc gcatactgga tcagcggctt gtccaccacc ggcatcatct
     8941 ccttggggct ggccttggtc gcgggcagaa aacggctccc catgcccgcc acagggaaga
     9001 ccgccttggt gattttcttg ctgcgccgat cgaagcggct gtgacgcttt tccgtgtatt
     9061 cgttatatcc cggaacggcc caattcatta ttcttctccc cctaacaagc ccatgaactt
     9121 gtcctcatcc aatactgcca cccccagtgc cagggccttt tccagcttgc tgcccgcttc
     9181 cgttccagcc accacgtaat cggtcttttt cgacacgctg ccactgacct tggctccgag
     9241 cgcctccagt ttttctttgg cctgatcgcg gctcatgccg ctcagcgtcc cggtcagcac
     9301 gaaggtcttc ccgctcaggt gcgattctcg cacagcctgc acctcgtgtt ccgcccattc
     9361 cacgccgcag gcgcgcaagg cgttgacgac ttcaagattg tgcggctcgg caaagaaagc
     9421 cagcagactc tgcgcgacga tgggcccgac atcgggcacc tgctgtaacg ccgcttcatc
     9481 cgcctgcatc aggttctcca gcttgccgaa gtggcgcgcc agatccttcg cggtcgcctc
     9541 gcccacattg cggatgccca gcgcatagat gaaacgcgcc agcgtggtgt gcttgctctg
     9601 ctcaatcgca tccagcaagt tcagcgcgga tttctcgccc atgcgctcca gatcgaccag
     9661 cgccgacatg cccaagtgat acagatcggc gggcgtatgc acgatgccgc cgtccaccag
     9721 ttgctcgacc agcttgtcgc ccaagccctc gatgtccatt gcgcggcgtc cggcgaagtg
     9781 cagcaacgcc tgcttgcgtt gcgccgggca gaacaggccg ccgctgcaac gccagtcggc
     9841 ctcgccctcc tcgcgcacga tatg
//
LOCUS       DASUIV010000210         9484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1167769, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000210 DASUIV010000000
VERSION     DASUIV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167769"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..2224
                     /locus_tag="VFW59_07650"
     CDS             257..2224
                     /locus_tag="VFW59_07650"
                     /inference="COORDINATES: protein motif:HMM:NF042647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein A"
                     /protein_id="HEX5364129.1"
                     /translation="MTSSESNNAAANVSSEDMVFPSFIYNRVDGVFVDLPRLLAADGG
                     LERFVERMFASGARFSGLDYDAFLKLLYDADWLVEMQSKATSLKLAESIVRFPPERQA
                     LYRKVKIGEGAKRAEYMFEPVHFDESYKEPVYGPAGADGIAPILSYETMIREVSTKLD
                     FDELVADMWLKGVKFGLREEAIRKIIASGDTIRLPIAMHLDPTASRDAEIIEVCADLH
                     RDNSPKLLANGKADLGVYKNRFPHVNKDTRLLKKVPRALGKPGYKVTGEIIEPEVPKD
                     LDLHPFAAAGTVVEQNEEGEFIVAAMDGFIGIDAKSNSISITEKIETKDGISAKTTGD
                     LALEVEEFIEHGEVQEGRTVKGKHMTFMADVFGNVISEGGNIYIAGNASGGRLEALDG
                     NITLASRATREVVRAIAGEVTATQCESSLLVGRIVRVEYAVNCEIIADEVYADTVEGC
                     IITGKLVKIKSSGERRGKETLVTMLIPDCSGVEQRIAAMQKQLGEMQAAIVGRLAGVE
                     TLKSDLEFAKFLGLHARIESGAIKLTNEQAVNWQKLVSKNAKAMQQMAALNKEIGTLE
                     KLVKDLEEEVSYSEREQDEMGNGVACEIETVVGHTVGQTLRSSAGITAFNGMSGGAIR
                     TSLQTMDAFKERIFIGDDGSIAWKFREAPVA"
     gene            2232..2795
                     /locus_tag="VFW59_07655"
     CDS             2232..2795
                     /locus_tag="VFW59_07655"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5364130.1"
                     /translation="MTDAHEMDLTETRLDTAVMYQGSFIEVRKDSVRLPDGRVSHREF
                     ITHPGAVAVLAMLDNGNLVMERQYRYAPQREFIELPAGKIDHGEDTLLTAQRELLEET
                     GYVASEWTHLATTWPCIGYADERMEYFLARGITHQGRNLDDGEFLEVFELSLSDALEW
                     IRLGKINDSKTIVGLFWLEKHLNHWRE"
     gene            2924..3451
                     /locus_tag="VFW59_07660"
     CDS             2924..3451
                     /locus_tag="VFW59_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5364131.1"
                     /translation="MTQYSKRMVMVHWLTLALLVAAWFLGDELAESTDESMATLAGYL
                     VHGAVGAAVLLLTLSRLLFRRKDGTPAPVGSGLMDKVATGIHHALYTVLFLLPVSGMM
                     TVLTSDAGKALLAGDAALLPKDGGYEHVFAHEVHEVLVTVLIVLVVIHVLGAIKHQFI
                     MKDGLMERMSLRRKD"
     gene            complement(3605..3793)
                     /locus_tag="VFW59_07665"
     CDS             complement(3605..3793)
                     /locus_tag="VFW59_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364132.1"
                     /translation="MYTILSRALPDAAQKEAITDCILIDFNLLKKQAAQQKHPWAAAL
                     KQCQALLPTKLSTDFVGK"
     gene            complement(3926..4816)
                     /locus_tag="VFW59_07670"
     CDS             complement(3926..4816)
                     /locus_tag="VFW59_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364133.1"
                     /translation="MLVRLRREYAQLITSGAQLLLLLVGAQLESRAGWLSTLSLMALV
                     SFVAWLSALYRLRTIRDTPTSKVASAAQGYVELSGLGQPFGDAPLISQLRMLPCLWYR
                     YKIEQHTREDKWKVIDSGESSDSFLLRDNSGDCIVDPEHAELITKHRDQWRDGDYRYT
                     EWKLIKGDSLYVIGEFRTHSGAIAFDDRKELIALLAEWKRDMPALHARFDLDRNGELD
                     MQEWMLARQAAKREVAKMQRAAQALPDVHLIGPPRDGKLFLISNLTPERLARRYLFWS
                     MAHLAIFFGALLGISWWLSH"
     gene            complement(4822..5388)
                     /locus_tag="VFW59_07675"
     CDS             complement(4822..5388)
                     /locus_tag="VFW59_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5364134.1"
                     /translation="MNSLIFFGLIAVALLYGITLYNHLVNVKHAVAKAWSNIDVLLKQ
                     RHDELPKLVEVCRQYKEFEQSTLQKVIEARSQVQSAREHQDVTALGQAEGLLRAGLGN
                     IFAVAEAYPELKANENFMQLQNRITGLENTIADRRELYNEAVNIYNVDIEQFPAVLIA
                     NLFHYEAKPLLEFSASEKTDVDMKQLFS"
     gene            complement(5463..6209)
                     /locus_tag="VFW59_07680"
     CDS             complement(5463..6209)
                     /locus_tag="VFW59_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5364135.1"
                     /translation="MSISIDISEQSGVRYLHFGSTWIQGAMRIARPWNLELEYTREMM
                     ASLLLRSPPRKVLLIGLGAASLTKFLYRNCPQAHLTVVEIEPGVVAAARQFFKLPEDS
                     RRINLVIGDGVEYVLGTDKKFDLILVDGFNEHAHPGALNTLPFYQACSTRLSDKGIMA
                     VNLLGLSRGSMGGFTHILKAFDDRALLLPSCASGNTIALAATGTPISIPFDELQKNAS
                     ALKTETGLNLLPTLTRLAQANTCIDDTLVL"
     gene            complement(6262..7083)
                     /locus_tag="VFW59_07685"
     CDS             complement(6262..7083)
                     /locus_tag="VFW59_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5364136.1"
                     /translation="MPHSTEIRPEQSIELLKELHILTRDGKLNQDSRRKLKQVYHLFH
                     FIEPLLTEVLSTGDSLTLADHGAGKSYLGFILYDLFMKAHGSGHIYGIETREELVERS
                     RMLADKLGFARMSFLNLSVEDSIQSPLLPERIDVVTALHACNTATDDAIKFALHKQAR
                     FIVLVPCCQAEVAAALNRNKNQSFGKTPLSEIWRHPIHTREFGSHLTNVLRCLLLESH
                     GYQVTVTELVGWEHSMKNELIIAKRQGAPRKNSRERLEHILHELNLQELQERFAY"
     gene            7307..8914
                     /locus_tag="VFW59_07690"
     CDS             7307..8914
                     /locus_tag="VFW59_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5364137.1"
                     /translation="MRIGVRLAIGFSVVVVVFLANLLQVGVSFSRLMQDIGHIKNETL
                     PYVLVVDQMDTSRSEVQQWLTDVSATHNRDGYKDAEEAAKKFLDGVAKYKEMYQREND
                     ADNLHKMEAIEVSFKDFYATGKEMAEAYITHGLDAGNLIMERFDGDSEAVSKRLAEFR
                     EQQVTEAGQRTAGALELADSTLRLMIGGGLVATLLGIVCSVLITRSVVRPVGRMRSTM
                     VEVGKNSDFTRRIAVDSGDEIGQTANSFNELMGALQATFSQMHDNIDKIFAASHQLAT
                     SSHQVATSSGHQSEATSAIAATLEQVTVSISHVSDRAHEALEMSRNSGGLSEKGGEII
                     HNAATEMVQIADTVRQTSGSIEALGQQSTQISSIVQVIKEIAEQTNLLALNAAIEAAR
                     AGEQGRGFAVVADEVRKLAERTSKATEEISQMIEEVQNTTHGAVANMSGTVRLVDGGV
                     ALAQQAGDAINQIKEESNKVLKTISDISAALEEQSSASNDIAIHVEKVAHMTDANRAA
                     TDGAAGAADDLADLAEVMRTAVNRYKF"
     gene            8962..>9484
                     /locus_tag="VFW59_07695"
     CDS             8962..>9484
                     /locus_tag="VFW59_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX5364138.1"
                     /translation="MASWTKKLMMVALAASNAAMAEEAPDWRAETLSGDWGGARSSLY
                     DKGVSVDITHKSDLLANTSGGRARGGVWLMNTEAAVGLDMDKLAGWRGGSAFVQYHVQ
                     HGDQPKNFNQTYVGSFAGVSNIETGTSTGQFYQAWLQQNSADDRLSLLGGLYAIDSEF
                     YVTESSGLFIQPPY"
BASE COUNT         2065 a         2212 c         2952 g         2255 t
ORIGIN      
        1 ttagcgtgaa tggcctgggg gtcctgatgc tgggcttctt gcccggttcg ttgatgtcgg
       61 tttgtgctgt gtcggtgcag caatcgttgc tgctgcatta ggctgaaccg agacagggtt
      121 aatccaatga ctcccgctgc ggcgggagtc attgtttgca aggaatattg tttgagtggc
      181 ggcgccgaaa tcgggtaatg tagccacacc gcgcttaaag caggcgtttt acaacggaga
      241 gaattgaaga gccaaaatga cttcatccga atcaaataat gccgcagcca atgtctcatc
      301 ggaagatatg gtgtttccca gttttatcta taaccgggta gatggggtgt tcgtcgattt
      361 gccgcgtctg ttggcggctg acggcgggct ggagcgtttc gttgagcgca tgtttgctag
      421 cggggctcgt ttttccggtc tggactacga cgcatttctc aagttgctgt atgacgcaga
      481 ctggttggtg gagatgcaat ccaaggcgac gagtctgaag ttggccgaga gcatcgtgcg
      541 ctttccgccg gaacgccagg ctctttatcg caaggtcaaa atcggcgaag gagccaagcg
      601 cgccgaatac atgtttgaac cggttcattt cgacgagtcg tataaggagc ctgtctatgg
      661 gccagccggg gctgacggta tcgcgcccat actgagttac gaaaccatga ttcgggaagt
      721 gtcgaccaag ctggacttcg atgagttggt tgcagacatg tggttgaagg gcgtgaagtt
      781 cgggctgcgc gaagaggcga tccgaaaaat catcgcgagc ggcgatacca tacgtctacc
      841 gattgcaatg catctcgatc cgacggcgag tcgtgatgcc gagatcatcg aggtgtgcgc
      901 cgacttgcat cgtgacaatt cgccgaagct cttggcgaac ggcaaagcgg acttgggggt
      961 gtacaaaaat cgcttccctc acgtgaacaa ggatacccgt ctgcttaaga aagtgccgcg
     1021 cgcactgggc aagccgggct acaaagtgac cggcgagatt atcgaaccag aagttcccaa
     1081 ggacttggat ttgcatccct ttgccgctgc cggcacggtc gtcgagcaga atgaggaggg
     1141 tgagttcatc gtggcggcca tggatggatt catcggcatt gatgccaaat cgaacagcat
     1201 ctccatcacc gagaagattg agaccaagga tgggatcagc gcaaagacga ccggagatct
     1261 ggcactggag gtagaggaat tcatcgaaca cggcgaggtg caggaaggcc gtacggtcaa
     1321 aggcaagcac atgaccttta tggcggacgt ctttggtaac gtcatctcgg agggcggcaa
     1381 tatttatatc gcaggcaatg cctcgggcgg gaggctggag gctctggatg gaaatattac
     1441 tcttgctagc cgtgccacgc gagaggtggt acgtgcgatc gcgggcgagg tgacggctac
     1501 tcagtgcgaa agcagcttgc tggttggcag gattgtgcgc gtggagtatg cggtgaactg
     1561 cgagattatc gccgatgagg tgtatgccga taccgtggag ggttgcatca tcactggcaa
     1621 gctcgtcaag atcaaatctt ccggcgagcg gcgcggcaag gaaaccttgg tgaccatgtt
     1681 gattccggat tgttccggag tagagcagcg tatcgccgcc atgcagaaac agctgggcga
     1741 gatgcaggcc gccatcgtcg ggcggctggc tggggtagaa acgctgaaat cggatctgga
     1801 gttcgccaag tttctgggct tgcacgcgag aattgaaagc ggcgcgataa agctcaccaa
     1861 tgagcaggcg gtcaattggc agaagctggt gagcaagaat gcgaaagcga tgcagcaaat
     1921 ggctgcattg aacaaggaaa ttggcacgct ggaaaaactc gtcaaagatc tcgaggagga
     1981 ggtttcttat tccgagcgcg agcaggatga aatggggaat ggcgttgctt gcgagatcga
     2041 aactgtcgtt ggacataccg tcggacagac cttgcgttca tctgcgggaa taacggcctt
     2101 taacggtatg tccggtggcg ccatcagaac ctcgctgcaa accatggatg ccttcaagga
     2161 gaggattttc attggggatg acggttcgat tgcctggaag ttccgggaag cacctgttgc
     2221 ttgaattggt catgaccgac gctcatgaga tggatttaac cgagacgagg ctggatacgg
     2281 ctgtaatgta tcagggaagc ttcatcgagg ttcgcaagga tagtgtgcgt ttgcccgatg
     2341 gcagggtgag ccatcgcgaa ttcatcactc atcccggggc ggtcgcggtg ctggcgatgc
     2401 tggataacgg caatctggtg atggagcggc agtaccgtta tgcgccgcaa cgcgaattca
     2461 tcgaactacc cgcaggcaag atcgaccacg gcgaagatac tctgctcacc gcacaacgtg
     2521 aactgctgga ggaaacgggt tatgttgcca gcgagtggac gcatcttgcc acaacctggc
     2581 cttgtatcgg ctatgccgac gagcgtatgg agtattttct ggcgcggggc atcactcatc
     2641 aggggcgcaa tctcgacgat ggcgagtttc tggaggtgtt tgaactgtcg ttatccgacg
     2701 cgctggagtg gatacgtttg ggcaagataa atgacagcaa gacgatcgtc ggcctgttct
     2761 ggctggagaa gcatctcaat cattggcgag agtgaattca gccgcgattt gaaatgtcgg
     2821 catggctcag taatttcggg taaattgcgc ccgttttcta acgatggaac tttgatggga
     2881 tggactcatc tcaccggttc gtaaaatttc taggagtgag gttatgacgc agtacagtaa
     2941 acggatggtg atggttcatt ggttgacctt ggccctgctg gtcgccgcat ggttcctcgg
     3001 agatgaattg gcagaatcga ccgatgagag catggcgacg ctggcaggct atctggtgca
     3061 tggggcggtg ggcgcggcgg tgctattgct gaccctctca cgcttgttgt tccgtcgcaa
     3121 ggatggcacg ccagcgcctg tgggcagcgg tttgatggac aaggttgcga cgggcatcca
     3181 ccatgcattg tataccgtgc tgttcttgtt gccggtgagt gggatgatga cggtgcttac
     3241 cagcgatgcc ggtaaggctc tgctggccgg ggatgcggcc ttgctgccga aggatggcgg
     3301 ttatgagcat gtgtttgccc acgaggttca cgaagtgctg gtgacggtgt tgatcgtgct
     3361 ggttgtgatc catgtgctgg gggcgatcaa acatcagttc atcatgaagg atggtttgat
     3421 ggagcgcatg tcgctacgcc gtaaagatta aggcgtcgtt tgactggcag agaagggcgg
     3481 tgttgcaacc gccctttttt attggcaggc atttaatgta ccccttgctg tgagtgtgac
     3541 cgcagttatt gctcatgctt attcgagaaa attcgagcta acccaccaac aggaaagagt
     3601 atttttattt acccacaaaa tctgtggata actttgtggg taacagtgcc tggcactgct
     3661 ttaatgccgc tgcccatggg tgtttttgtt gcgctgcttg ttttttaagt aaattaaaat
     3721 ctattaaaat acaatccgtt atggcttctt tctgggcggc atctggcagc gcgcgggaaa
     3781 ggatggtgta caagctggct ttgtgaatga tttttcgttg tcaatcttta ttttgggtgt
     3841 tttttacgct gctctgcgcg acttttccct cggctaccgg cttgatttat ctaggccgtg
     3901 aggttcggaa atgttcagtc gtctctcagt ggctcagcca ccagctgata ccgagtagcg
     3961 caccaaaaaa tattgccaga tgtgccatgc tccagaacag gtagcggcgc gccagcctct
     4021 ctggggtgag gttgctaatc agaaaaagtt tgccgtcgcg cggcggaccg atgaggtgga
     4081 catcgggcag ggcttgagcc gcgcgctgca tttttgccac ttcacgcttg gccgcctggc
     4141 gagccagcat ccattcctgc atgtccagtt cgccgttgcg gtcgagatcg aagcgggcat
     4201 gcagtgcggg catgtcgcgt ttccattcgg ccagcaaggc aatgagttcc ttgcgatcat
     4261 cgaatgctat cgcaccgcta tgtgtgcgga attcgccgat gacatacagg ctgtcgccct
     4321 tgatcagctt ccattcggtg taacgataat caccatcgcg ccattggtcg cgatgcttgg
     4381 tgatgagttc tgcatgctcc ggatcaacta tgcaatcacc gctgttgtcc cgcagcagga
     4441 acgagtcgct gctctcgccg ctgtctatga ctttccattt gtcttccctg gtatgttgct
     4501 cgatcttgta gcgataccac aggcagggca acatcctgag ctggctgatc aacggggcgt
     4561 ctccaaaggg ctgcccgagt ccgctgagct cgacatagcc ctgtgccgcc gaggcgactt
     4621 ttgaagtcgg tgtgtcgcgt atcgtgcgta atcgatacag agcagaaagc caggcgacga
     4681 agctgacgag agccatcagc gaaagcgtgc ttaaccagcc tgcgcgtgat tccagctgtg
     4741 cgccgacgag cagcaacagc agttgtgcgc cggacgtgat cagttgcgcg tattcgcggc
     4801 gcagccggac tagcatggtg cttagctgaa cagttgtttc atgtcgacat ccgttttctc
     4861 gctggcggag aattccagca gcggcttggc ttcatagtga aacaggttgg caatcagtac
     4921 ggcagggaat tgttcgatgt ctacgttgta gatattcacg gcttcgttat acagctcgcg
     4981 gcggtcggca atcgtgtttt ccagtccggt gatgcggttc tgcagttgca tgaagttctc
     5041 gtttgccttc aattcggggt aggcttctgc taccgcaaag atgttgccca gccctgcgcg
     5101 caacaatcct tccgcctgcc ccagtgcggt gacatcctga tgttcacgcg cggattgcac
     5161 ctgcgagcgt gcctcgatca ccttttgcag ggtgctctgc tcgaattcct tatattgcct
     5221 gcatacttcg accagttttg gcagttcatc gtgccgctgt ttgagcagca catcgatgtt
     5281 ggaccacgcc ttggcgacgg catgctttac gttcaccaga tggttgtaca gggtgatgcc
     5341 atagagcagc gcgacggcga tcaggccgaa aaaaatcaga gaattcatgt tcgctcccga
     5401 atgaggttgg atgtgccctc atgctaatgc aaaatgggag ctcggtgtat ggctggcgag
     5461 ggttacagaa ccagtgtgtc gtcaatgcag gtattggctt gcgctaaccg cgttagcgtc
     5521 ggtagcaggt tcaggccagt ttccgtcttt aaggcagagg cattcttttg gagttcgtcg
     5581 aatggaatgc tgatcggggt tcccgttgcg gcaagcgcga tggtattgcc gcttgcgcag
     5641 gaaggaagca gaagtgcgcg atcatcaaat gccttcagaa tatgcgtgaa gccccccatg
     5701 gagccccggc tgaggccgag cagattgacg gccatgatgc ccttgtcgct taatcgggtg
     5761 ctgcatgcct gatagaacgg cagggtgttc aaggcgccgg ggtgcgcatg ctcgttaaat
     5821 ccatccacca gaatcaggtc gaatttcttg tcggtgccaa gcacatactc gacgccgtcg
     5881 ccgatcacca gattgatgcg tctggaatct tccggcagct tgaagaactg ccgtgccgcc
     5941 gcgactaccc cgggctcgat ctctactacg gtgaggtggg cctgcggaca gttgcgatac
     6001 agaaatttgg tcaacgaggc tgcgccaaga ccaatcagta gaaccttgcg ggggggcgaa
     6061 cgtagcagca acgaagccat catttcgcgg gtgtattcca gttccagatt ccaggggcgt
     6121 gcgatgcgca ttgcgccttg tatccatgtg gagccgaaat gcagatagcg aacgccggat
     6181 tgttcggaaa tgtcgataga gatactcatg tggaaatttg ttttggatgt gtgaacggta
     6241 acgatagggg cggcagtggg gttaataggc gaatcgttct tgcagttcct gtagattcaa
     6301 ttcatgcagg atgtgttcca gacgttcgcg cgaatttttg cgcggggcac cctggcgctt
     6361 agcgatgata agctcgttct tcatcgagtg ctcccagcct accagttccg tgaccgttac
     6421 ctgatagccg tgcgattcca gcagtaaaca tctcagtacg ttggtgaggt ggctgccgaa
     6481 ttcgcgggtg tggatggggt ggcgccatat ttcggatagc ggcgttttgc cgaacgattg
     6541 attcttgttg cgattcaatg ccgccgcgac ttcggcctgg cagcagggaa ccagcacgat
     6601 aaatctggcc tgtttatgca gtgcgaattt gatggcatcg tcagttgccg tgttgcaggc
     6661 atgcaatgcg gtaacgacat cgatgcgctc gggtaatagc ggcgactgaa tggagtcttc
     6721 taccgacagg ttgagaaagg acatgcgcgc gaagccgagt ttgtcggcca gcatgcgtga
     6781 tcgctcaacc agttcttcgc gggtttcgat gccatagatg tgtccggacc cgtgtgcttt
     6841 catgaacagg tcgtacagaa tgaagccgag ataagacttc cctgctccgt gatccgccag
     6901 ggtgagcgag tcgccggtgc tcaagacttc ggttagcaag ggttcgatga agtggaacag
     6961 gtggtaaacc tgcttgagct tgcggcggct gtcttggttg agtttgccat cgcgcgtcag
     7021 gatatgcagc tccttgagca gttcaatgga ttgttcgggt ctgatctcgg tagagtgggg
     7081 catggcgtat ttggctaatg gctaacagcc gacattctac aagcaccgag cttggcaagt
     7141 gcaatgcggg cgtgaaaggg gctgggcgcg ttgatgagac ttatgcacaa gcctgtttgt
     7201 tgagggtgaa gggctggggt agaataacgc actatacctt tttagcccgg ctggtcagtg
     7261 actgcggaat tcaaggttgt tcagggagat tatataagtg actaatatgc gtattggagt
     7321 gcggcttgct atcggatttt cggtagtggt tgtggttttt ttggcaaatc tattgcaggt
     7381 gggcgtgtcg ttctcccggc tcatgcagga catcgggcat atcaagaacg agaccttgcc
     7441 gtatgtgctg gtcgtcgatc aaatggacac gagtcgttcc gaggttcagc aatggctgac
     7501 cgatgtgtct gcgacgcata accgcgatgg ttataaggat gccgaagagg cggccaagaa
     7561 atttctggat ggcgtagcta aatacaaaga gatgtaccag cgggaaaatg acgctgacaa
     7621 tttgcacaaa atggaggcga tagaagtcag tttcaaagac ttctatgcaa caggcaagga
     7681 gatggctgaa gcctatatca cccatggtct ggatgccggc aacttgatca tggagcgctt
     7741 cgatggggat agtgaggctg tttccaagcg gctggccgag tttcgtgagc agcaggtgac
     7801 cgaggctggg caacggactg caggagcgct ggagttggct gattccaccc tgcgtttgat
     7861 gatcggtggc ggtctcgtgg ctaccttgtt gggaattgtg tgctcggtgt tgatcactcg
     7921 ctcggtggtg cgccccgttg ggcggatgcg ttctacgatg gtcgaggttg gaaagaatag
     7981 tgatttcact cgccgtatcg cagtagacag cggtgacgaa atcggtcaaa ccgccaactc
     8041 gttcaacgaa ttgatggggg cgttgcaagc caccttttcc cagatgcacg acaacataga
     8101 caaaatattc gcagcttcac atcagttggc cacgtcttcc caccaggttg cgaccagttc
     8161 ggggcatcaa agcgaagcga cgtcggctat cgcagcgacg cttgaacaag ttaccgtaag
     8221 tatcagtcat gtttccgatc gtgcgcatga agcgctggag atgtcccgta attctggcgg
     8281 gctgtctgag aagggcggtg agattattca caacgcggct accgaaatgg ttcagatcgc
     8341 ggataccgta cgccagactt ccggatccat tgaggctctg gggcagcagt cgacacagat
     8401 ttcctcgatt gttcaggtta tcaaggaaat cgccgagcaa acgaatttgc tggcgttaaa
     8461 tgccgctatt gaggcggctc gtgctggtga gcaagggcga ggttttgctg ttgttgcgga
     8521 cgaggtgcgc aaactggccg aacgcaccag caaggccacc gaggaaatca gtcagatgat
     8581 tgaggaggtt caaaatacta cgcacggtgc cgtggccaat atgtcgggta cggtcaggct
     8641 ggtggatggg ggcgtcgcct tggcgcaaca ggctggtgac gcgattaatc agatcaagga
     8701 agaatcgaac aaggtgctta agacgatcag cgatatttcc gctgcgttgg aagagcagag
     8761 tagcgccagc aatgatattg ccattcatgt cgagaaggta gcgcacatga cggatgcaaa
     8821 cagggcggcg acagatggtg cggcaggcgc cgcagatgat ctggccgact tggcggaagt
     8881 gatgcgtacg gcagtgaatc gatacaaatt ctagttcgct gtttaagtga tggcgtggtg
     8941 aaacgataag gggagtgaac gatggcatct tggacgaaaa aactcatgat ggtcgcgctg
     9001 gctgcgagca atgcggcaat ggcagaggag gcgcccgact ggcgtgcaga aacactgagt
     9061 ggggattggg gtggagctcg ctccagcctc tacgataagg gtgtttcggt ggatatcacg
     9121 cacaagagcg atctgttggc gaacacctcg ggtgggcggg ctcgcggcgg ggtgtggctg
     9181 atgaacaccg aggctgcggt gggccttgat atggataagt tggcgggctg gcgtggaggc
     9241 agtgccttcg ttcagtatca tgttcagcat ggcgatcagc ccaaaaactt caatcaaaca
     9301 tatgtaggca gtttcgccgg tgtgagtaac atcgagacgg gaacgagtac ggggcaattt
     9361 tatcaggcgt ggttgcagca gaactcggct gacgatcgtc tctcgctgct gggggggcta
     9421 tacgccatcg attcggaatt ctatgtgact gagagctccg gattgtttat tcaacctcct
     9481 tatg
//
LOCUS       DASUIV010000211         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_423670, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000211 DASUIV010000000
VERSION     DASUIV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423670"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2406)
                     /locus_tag="VFW59_07700"
     CDS             complement(<1..2406)
                     /locus_tag="VFW59_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5364139.1"
                     /translation="MTTWHQEYRILHPVKGVRWMESNTNPQPHPDGGVIWYGYVFDIT
                     ERKRAEEVLRFIAQGEWLNSGEIFLNALVRYLGQILNVDYVVIDKIASDPAYAETVAL
                     YAKGEVAPNIRYPLQGTPCANVMTGNLCSYPVNVQGLFPEDGLLVEMQAVSYVGLPLW
                     DTNGKVIGLIAVLDGKPMADSNPVKSILQLVATSAAAELKRSLMEQVLRESHESLREA
                     QRISHVGNWELDLASNTLHWSDEIYRIFEIAPEQFGNSYEAWISFVHPEDREAVDRAY
                     TESVKNHTPYEIEHRALLADGRIKYVQERCETLYDQDGRALRSIGTVQDITERKQLEA
                     ELRASRNFLDSVIDSVSDPIFVKDRQHRWTLLNDAFCNFIGKPREALLGKSDYDFFPK
                     EQADVFWEKDELVFESGQGNLNEESFTSAGGEEHFIQTRKTPFVAGDGREMLVGVIRD
                     ISDLKRYEVAREAALAEAVRLAKLRSEFLAHMSHELRTPLNGILGYAQILQRNKSLGE
                     QHAVALNVIRQSGEHLLALIDDILDLARIESGKLELVMSDIPLARFLRVVAEIVAVRA
                     RQKSVEFSCELAPDLPEAVLGDEKRLRQVLLNLLSNAIKFTDQGKVTLCVSRASPSRL
                     AFAVKDTGIGIEPSDQETIFQSFEQSGDAQHRLGGTGLGLAISRKLVRLMGGDIQLES
                     RVGYGSTFRFELVLPAVNLAPVALLPAQIATGYAGARRKVLIADDLAENRALVLDLLT
                     PLGFAMIEASDAHEALELARSMSPDLMLMDASLSSMDFCEAIRRLRNMPELMHMPIVV
                     LSAE"
BASE COUNT          476 a          742 c          702 g          513 t
ORIGIN      
        1 ctccgcactc agcacgacga tgggcatatg catcaattcc ggcatgttgc gcaggcgacg
       61 aatcgcctcg caaaaatcca tagaggacag gctggcatcc atcagcatca gatcgggcga
      121 catcgaacgg gccagttcca acgcctcatg cgcatcgctc gcctcgatca ttgcgaatcc
      181 cagcggggtc agcagatcga gcaccagggc gcgattctcg gcaagatcgt cggcaatcag
      241 caccttgcgg cgcgccccgg cataaccggt agcgatctgc gccggaagca gcgctaccgg
      301 tgcaagattc acggcaggca acaccagctc gaagcggaag gtgctgccat aaccgacgcg
      361 gctctcgagc tgaatatccc cgcccatcag acgcaccagt ttccggctga tggccagccc
      421 cagccccgtg ccgccaagcc ggtgttgcgc atcgcccgac tgttcgaagg actggaagat
      481 cgtttcctga tcggatggtt cgatgccgat accggtatcc ttgacggcga aggccagtcg
      541 cgacgggctc gcacggctga cgcacagggt gaccttgcct tgatcggtaa atttgatggc
      601 attggacagc aggttgagca acacctgacg cagccgcttt tcgtcgccca ggacagcctc
      661 cggcagatcg ggtgccaatt cgcaggaaaa ctcgacactc ttctgccgcg ccctgaccgc
      721 aacgatctcg gcgacgacgc gcaggaacct ggccagcggg atatcgctca tgaccaattc
      781 cagcttgccg gattcgatgc gggccagatc gaggatgtcg tcgatcagcg ccaacagatg
      841 ctcgccgctc tggcggatga cgttgagtgc gacggcatgc tgctcgccca gcgacttgtt
      901 gcgttgcaat atctgcgcgt agccgagaat gccgttcagc ggcgtgcgca gttcgtggct
      961 catgtgcgca aggaactcgc tgcgcagctt tgccagccgc acggcctcgg ccagcgcggc
     1021 ctcgcgtgcc acctcgtagc gcttgagatc ggagatgtcc cgtatcaccc cgaccagcat
     1081 ttcgcggcca tcgcccgcaa cgaacggggt cttcctggtc tggatgaaat gttcttcgcc
     1141 gcccgccgag gtgaaacttt cttcgttcag attgccctgt ccggactcaa acacgagttc
     1201 gtctttttcc cagaacacat cggcctgctc tttggggaag aaatcgtaat cggacttgcc
     1261 gagcagcgcc tcgcgcggct tgccgatgaa gttgcaaaac gcatcgttga gcagcgtcca
     1321 gcggtgctgt ctgtccttga cgaagatcgg gtcggaaacc gagtcgatca cgctgtcgag
     1381 gaagttgcgc gatgcccgca gttcggcttc cagttgtttg cgctcggtga tgtcctgcac
     1441 ggtaccgatg gagcgtagcg cccggccatc ctgatcgtag agggtctcgc aacgttcttg
     1501 cacatatttg atccgcccgt ccgccagtaa cgcgcgatgt tcgatctcgt agggggtatg
     1561 gtttttcacc gactcggtat aagcgcgatc caccgcctcg cggtcttcgg gatgaacgaa
     1621 gctgatccaa gcctcataag aattgccgaa ttgttcgggt gcaatctcga agatgcggta
     1681 aatttcatcc gaccagtgca atgtgttgct ggccagatcc agttcccagt tgccgacgtg
     1741 ggaaatgcgt tgcgcctcgc ggagcgattc gtggctctcg cgcaggacct gttccatcag
     1801 ggaacgtttc aattccgccg cagcactggt cgccaccagt tgcaggatgg atttcaccgg
     1861 attgctgtca gccataggct tgccatccag cacggcgatc aggccgatca ctttaccatt
     1921 cgtgtcccac aacggaagtc ccacataact cacggcctgc atctctacca gcaggccatc
     1981 ttcggggaac agcccttgaa catttaccgg gtagctgcac aggtttcctg tcatcacatt
     2041 ggcgcaaggg gtgccttgaa gcgggtagcg gatattggga gcgacttcgc ctttggcata
     2101 gagcgccacg gtttcggcat aggccgggtc ggatgcgatc ttgtcgataa ccacataatc
     2161 gacattcagt atctggccca gataacgcac cagcgcattc aggaagattt cgccgctgtt
     2221 cagccactcg ccttgggcaa taaatctgag cacctcctcg gcgcgcttgc gctcggtgat
     2281 gtcgaacaca tagccgtacc agatcacccc gccgtccggg tgtggttgcg ggttggtatt
     2341 gctttccatc cagcgtaccc ctttgacggg gtgcaatatc cggtattcct ggtgccaggt
     2401 cgtcatggtt cgcgccgact cggcgatggt ttc
//
LOCUS       DASUIV010000212         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_702862, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000212 DASUIV010000000
VERSION     DASUIV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702862"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1853
                     /gene="typA"
                     /locus_tag="VFW59_07705"
     CDS             39..1853
                     /gene="typA"
                     /locus_tag="VFW59_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5364140.1"
                     /translation="MSRALRNIAIIAHVDHGKTTMVDKLLSQAGTWSAHQMVAERVMD
                     NNDLEKERGITILAKNCAVNYEGVHINIVDTPGHADFGGEVERVLGMVDGVLLLVDAV
                     EGPMPQTRFVTKKALALGLRPIVVINKVDRPGARPDWVIDQTFDLFDKLGATEEQLDF
                     PVVYASALNGYATLDLAKPSTDMRPLFDAVLKNVPAPSAAAIDEPLQLQISSLEYSSF
                     VGRIGVGRVLRGTIKPGQDVMVMNGDKSSKRGRINQVLGFQGVDRILLTEAKAGDIVL
                     INGIEEIGIGVTIADINKPEALPMPKVDEPTLTMNLMVNTSPLCGQEGKFITSRQIRD
                     RLTKELLVNVALRVEETDNTDVFLLAGRGELHLTILLENMRREGFEMGVGKPRVVYRD
                     IDGVKCEPFEVLTVDVEDANQGAIMEELGRRRGDLQDMQPDGHGRVRLEYRIPARGLI
                     GFQSEFMTLTRGTGIMAHVFDDYAPVKADMPGRRNGVLISAEQGDAVAYALWKLEDRG
                     RMFVSPGDKLYEGMIIGIHSRDNDLVVNPIKGKQLTNVRSSGTDEAVRLTPPIRLTLE
                     SAVEFIDDDELVEITPLSVRMRKRYLTENERKRQGRGL"
     gene            2067..>3351
                     /gene="rmuC"
                     /locus_tag="VFW59_07710"
     CDS             2067..>3351
                     /gene="rmuC"
                     /locus_tag="VFW59_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX5364141.1"
                     /translation="MRGEAQVEMARLNERLSAAQEDVRRLIEEHAESEDGLRQLKQEL
                     GDLREKLGAAESTVANQATQLLALQREKEELAALRDRLTAEQQRLSNQVTELTTSLDA
                     ERSQGLEKLQLLQNAEAQLTDRFKTLASEILEDKSKRFTEQNQTNINQLLEPLKVRIT
                     EFQGKVQEVYVQEGKDRSALAEQVKQLMALNNQLSADAHNLTRALKGQAKTQGNWGEL
                     ILERVLEASGLRRGHEYDVQESHTRADGSRAQPDVVVHLPEDRHLIVDAKVSLNAYEE
                     YANAETDPQRDAAMKRHLDSVRGHIKDLSEKNYQQLYGLQSLDFVLMFIPVEPAFMLA
                     ISHDSDLWQDAWKKNVLLVSPSTLLFVVRTVAHLWRQEQQNRNAQDIASRGAELYDKL
                     AGFVDDLENLGAKLQQAQKAYDGAYGKFTGGRGNVI"
BASE COUNT          703 a          916 c         1084 g          648 t
ORIGIN      
        1 agaatgcgcg ccctttgaac tttgcaggta gcagaaccat gtcccgcgca ctccgtaaca
       61 tcgccattat cgcccacgtt gaccacggca aaaccaccat ggtcgataag ctgctctccc
      121 aagctggtac ttggtccgct caccagatgg ttgccgagcg cgtgatggac aacaacgatc
      181 tggaaaagga acgcggcatc accattctgg cgaagaactg tgcggtgaat tacgagggtg
      241 tgcatatcaa catcgtcgac acaccgggcc acgccgactt cggcggcgag gtcgagcgcg
      301 tgctgggcat ggtggacggc gtattgctgc tggtggatgc ggtcgaaggc ccgatgccgc
      361 agacccgttt cgtgaccaag aaggcactgg ccctcggtct gcgtcctatc gtggtgatca
      421 acaaggttga ccgtccgggt gcgcgcccgg actgggtgat cgaccagact ttcgatctgt
      481 tcgacaagtt gggcgcgacc gaagagcagc tggacttccc cgtcgtgtat gcctcggcat
      541 tgaacggtta cgccacgctc gatctggcca agcccagcac cgacatgcgc ccgttgtttg
      601 atgccgtgtt gaagaatgtg cctgcacctt ccgctgccgc catcgatgag ccgctgcaac
      661 tgcaaatctc ctcgctggaa tactccagct tcgtgggccg catcggtgta ggccgcgtgt
      721 tgcgcggcac catcaagccg ggccaggatg tgatggtgat gaatggcgac aagtcgtcca
      781 agcgcggccg catcaaccag gtgctgggtt ttcagggcgt cgatcgcatc ctgctgaccg
      841 aagcgaaggc gggcgacatc gtgctgatca acggtatcga agagatcggt atcggcgtaa
      901 ccatcgccga catcaacaag cctgaagcgc tgcctatgcc caaggtcgac gagccgaccc
      961 tgaccatgaa cctgatggtc aatacctcgc cgctgtgcgg ccaggaaggc aagttcatca
     1021 ccagccgtca gatccgtgat cgtctgacta aagagttgtt ggtgaacgtg gcgttgcgtg
     1081 tcgaggaaac cgacaacacc gacgtgttcc tgctggcggg tcgcggcgaa ttgcatctga
     1141 cgattctgct ggaaaacatg cgccgcgaag gcttcgagat gggtgtgggc aagcctcgcg
     1201 tggtgtaccg cgacatcgac ggcgtgaagt gcgagccgtt cgaagtgctg accgtggacg
     1261 tggaagatgc gaatcagggc gcgatcatgg aagagctggg ccgtcgtcgt ggcgacctgc
     1321 aagacatgca gccggatggt cacgggcgtg tgcgcctgga ataccgtatt cctgcgcgcg
     1381 gcctgatcgg cttccagtct gaattcatga cgctgacacg cggcaccggc atcatggcgc
     1441 acgtgttcga cgattacgcg ccggtcaagg ccgatatgcc gggacgccgc aacggcgtgc
     1501 tgatttccgc agagcagggc gacgccgtcg cttatgcgct gtggaagctg gaagatcgcg
     1561 gccgcatgtt cgtgtcgccc ggcgacaaac tgtacgaagg catgatcatc ggcatccaca
     1621 gccgcgacaa cgatctggtg gtcaacccga tcaagggcaa gcagctgacc aacgtgcgct
     1681 cctccggcac cgacgaggct gttcgtctga caccgccgat ccgtctgacg ctggagtctg
     1741 cggtcgaatt catcgatgac gacgagctgg tcgagatcac gccattgtcg gtgcgcatgc
     1801 gcaaacgtta cctgactgaa aacgaacgca agcgtcaggg ccgcggtctg taatttgcgt
     1861 tttagtctga cgtgaagaaa agggatgctc aggcatccct ttttatttgg atcggttgat
     1921 tgtcatatac tgataaaaaa gtttttgagg gagtagttat ggagcagttg ggcggcgttt
     1981 tcatttctct tttaatcggt ctgttgctcg gggttgccgt catttggctg gttttgcggg
     2041 agcgcatcgc gtctgtggag atgcgtatga gaggcgaggc tcaggttgag atggcgcgtc
     2101 tcaatgagcg tttgagtgcg gctcaggagg atgtgcggcg tctgatcgaa gagcatgccg
     2161 agtcggaaga cggtctgcga cagctcaagc aggagctggg ggatttgcgc gagaagctgg
     2221 gcgcggcaga gtccacggta gccaaccagg ccacgcagtt gcttgcgctg caacgcgaaa
     2281 aagaagagct cgccgcgctg cgcgaccggc tcactgccga acagcagcgc ctgagcaatc
     2341 aggtgaccga gctgaccacc agcctggatg cggagcgttc gcagggtctg gagaaattgc
     2401 agctgttgca gaatgccgag gcgcagctga cggatcgctt caagacgctg gccagcgaga
     2461 tactggaaga caagtccaag cgcttcaccg aacagaacca gaccaatatc aaccagttgc
     2521 tggaaccgct caaggtcagg atcaccgagt ttcagggcaa ggtgcaggag gtctatgttc
     2581 aggaaggcaa ggatcggtcg gcgctggccg aacaggtcaa gcagctgatg gcgctgaaca
     2641 accagctctc tgcggacgcg cacaacctga cccgcgcgct caaggggcag gccaagacgc
     2701 agggcaattg gggcgagttg atactggagc gggtgctgga ggcttccggc ttgcgccgtg
     2761 ggcatgaata cgacgtgcag gaaagccata cccgcgcgga cggttcgcgt gctcagccgg
     2821 acgtggtggt gcatctgccg gaagaccggc atctgatcgt cgatgccaag gtgtcgctca
     2881 acgcgtacga ggaatatgcc aacgcggaga ccgatcccca gcgcgatgcc gcgatgaagc
     2941 gtcatctgga ttcggtgcga ggtcatatca aggatttgtc agagaagaat tatcagcagc
     3001 tctacggcct gcaatcgctg gatttcgtgc tgatgttcat cccggtggag cctgccttca
     3061 tgctggcgat ttcgcatgac agcgatctgt ggcaggacgc ctggaagaag aacgtgctgc
     3121 tggtcagccc gagcaccttg ctgttcgtgg tgcgcaccgt ggcgcatctg tggcggcagg
     3181 aacagcagaa ccgcaacgcg caggacattg ccagccgcgg tgccgagctc tacgacaagc
     3241 tggccggttt cgtggacgat ctggaaaatc tgggggcgaa gctgcagcag gcgcaaaagg
     3301 cttacgacgg ggcctatggc aagttcaccg gcggtcgcgg caatgtgatc c
//
LOCUS       DASUIV010000213         5642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_887301, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000213 DASUIV010000000
VERSION     DASUIV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887301"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VFW59_07715"
     CDS             complement(<1..985)
                     /locus_tag="VFW59_07715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5364142.1"
                     /translation="MEIIQIVRRYGPTGGMERYVWELTHELARLGHRVVVLCEHQTTT
                     DKPDGIEVHELGTMFYRPRWLYYWRFGRRVERWLKQHPHPGWLIHSHERVGVHDVTTF
                     HGPPFASVRDKPWWKKISLRIAMQLFMERRELNTARAIVPNSEIIRRQLAHYYPEYAH
                     KLTPPVVPGVLPTVQRPARTVPPDGGVIGFVGYEWQRKGLPLAVAIAQELRRSRPKLE
                     LWVVGPEPDEVRALFAGWQDGYRLLGWRSDAEHLREFDLLLHPARAEPYGMVISEAMA
                     ARVPVVVSDRCGAAAQISTEAGIVVALDAPLQEWVTATTLQLNRTQPPPPFM"
     gene            1088..2083
                     /locus_tag="VFW59_07720"
     CDS             1088..2083
                     /locus_tag="VFW59_07720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5364143.1"
                     /translation="MDSITSSAKPLCDLCGAEGLLAQDKVADPDGCITGDWSFRRCSN
                     AACGVFWLDPAPLESELWKAYASYHTHTRKSSNKLTRAALSLAHRVLKFILLPVWLIN
                     GLKREADNLRFMTLADEPAGRLLDVGCGAGRFLNRMRKRGWEVEGVDFDPQAAQKVAA
                     RYAIKTHVGDLVTCALPADSFDVVTMSQAVEHLFDPRKNLQECLRILKPGGLLVMTTP
                     NVASRGAALFGAFWRGWEPPRHLHLFSVASLRQLTEQCGFEVVEAGTFSAGSAVVYRV
                     SRSNQLAHDGETANPLALLFWGYRQELSEYLDQQSACDTGQNVLIRARKPMQGAD"
     gene            2086..2925
                     /locus_tag="VFW59_07725"
     CDS             2086..2925
                     /locus_tag="VFW59_07725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5364144.1"
                     /translation="MFSIIIPTWNNLALLQLCIRSIRQNSAHAHQIIVHVNDGSDGTL
                     AWVREQGFAHTASPENIGICLAVNEAAMHATRDYILYLNDDMYCCPGWDSALIDRLGQ
                     LDTDLFMLSGTMIEPRDTNNPCVIVRDYGREVESFDEAALLAELPQHRKADWCGATWP
                     PTLVSRRWWFKVGGYSSEFSPGMSSDNDFSMKLWHAGCRVFLGVGDSLVYHFQCKSTG
                     KVKKNDGGKQFLNKWGVRQSVFDRYYLRRGQLAKGLALEEPEDTREFRWQMFRSRLKR
                     ALG"
     gene            complement(2922..3671)
                     /locus_tag="VFW59_07730"
     CDS             complement(2922..3671)
                     /locus_tag="VFW59_07730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5364145.1"
                     /translation="MPLLSVVLITKNEAANIRACLESVAWADEIIVVDSGSTDDTVAI
                     CREFTPHVFVHADWQGFGIQKNLALDHASKDWILSLDADERVTPELRAQLINAMTEGP
                     YDGFYLPRLSQFCGKFIRHSGWHPDYVLRLFKRGAARFSDDLVHESVQLQGPTGKLTS
                     PLLHYSYLTKDDVERKVAHYSDSAAQQMLRAGKRATSVDAPLRGGWAFLRTYFLRLGF
                     LDGLAGWDIARMNARTTYWKYRKLAALNSTR"
     gene            complement(3674..5509)
                     /locus_tag="VFW59_07735"
     CDS             complement(3674..5509)
                     /locus_tag="VFW59_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX5364146.1"
                     /translation="MLRKSNSRIVIVVLHDAIAAALAWNMAYLLRFNFDLPGNVVAEI
                     KHTLPWIVPLQLFIFWRFSLYRGLWRYASTTDLRRIVLGVALSAAAISLLLWMLRLGE
                     IIPRSVLVIDPLLLILLMGGGRFAYRMMKEHSLYSSIKLNSEPVLVLGAGDAAALLSK
                     DLAKSATWQQVGFLDDDKEKHNRTLNDVKILGSLDQLSHWAEHFNVEHVIIAMPSSSH
                     QQRKRAIHLCNQAGVKALTVPSFDDLMSGKVAISQLRPIELDDLLGRDPVELDNAGLH
                     GLLTGKVAMVTGAGGSIGSELCRQIARFSPAKLVLFELSEFALYTLEQELRQTFPTLN
                     FVCLVGDVRDADRVAEALRLHQPAVLFHAAAYKHVPMMEQHNAWQAIRNNVLGTHTVA
                     RAAQQHGVGKFVLISTDKAVNPTNVMGASKRLAEMACQALQPSSSTRFISVRFGNVLG
                     SSGSVIPKFRAQIAQGGPITVTHPEITRYFMSIPEAAQLVLQAGLMGRGGEIFVLDMG
                     EPVKIVDLAKDLIRLSGFSEDDIQIEFSGLRPGEKLYEELLANDENTLPTPHPKLRIA
                     QARQADAAWLEQLLKWTEASTMPDDQVRNALVEWVPEYTPTSNGH"
BASE COUNT         1098 a         1643 c         1762 g         1139 t
ORIGIN      
        1 gcatgaaagg cgggggtggt tgcgtccgat tcaattgaag tgtggtggct gtgacccatt
       61 cttgcagggg ggcgtccagt gccaccacga tgcccgcctc agtgcttatc tgtgcagcgg
      121 cgccgcatcg gtccgatacc accaccggca cgcgtgccgc catcgcttcg gagatcacca
      181 tcccataggg ttccgcgcgt gccgggtgca gcagcaggtc gaattcgcgc agatgctcgg
      241 cgtcggagcg ccagcccagc aggcgatagc catcctgcca gccggcaaaa agcgcgcgca
      301 cctcgtcggg ctccggcccg actacccaga gttcgagctt cgggcggctg cggcgcagtt
      361 cctgtgcaat cgccacggcc agcggcaaac ccttgcgctg ccattcgtaa cccacgaagc
      421 cgatcacgcc gccatcgggc ggcacggtgc gcgcggggcg ctgcacggtt ggcagcacgc
      481 ctggcacgac aggaggtgtg agtttgtgcg cgtactcggg atagtagtgc gccagctgcc
      541 gacgaatgat ttcggagttg ggcacgatgg cgcgagcggt attcagttcg cggcgctcca
      601 tgaatagctg catggcaatg cgcaaagata tcttcttcca ccagggcttg tcgcgcaccg
      661 aggcgaaggg cgggccgtgg aaggtggtga cgtcgtgcac gcccacgcgc tcatggctgt
      721 ggatcagcca gccgggatgc gggtgctgtt tcagccagcg ttccacgcgg cgaccgaaac
      781 gccagtaata cagccagcgt ggccgataga acatggtgcc cagttcatgg acttcgatgc
      841 cgtcgggttt gtccgtcgtg gtttgatgtt cgcatagcac cacgacgcga tggccgagcc
      901 gtgccagttc atgagtgagt tcccagacgt agcgttccat gcctcctgtc gggccgtaac
      961 gacggacgat ttgaattatt tccatgcaac ctctgacgcc tgctccgcaa ccaatataaa
     1021 atgcgggctc aaattaaatg cggcccgtgg caaatctttt gcccggccgc gacaggccac
     1081 taaaaagatg gacagtataa caagcagcgc taaaccctta tgcgatctct gtggtgccga
     1141 gggattgctg gcgcaggata aagtcgccga tcctgacggg tgcatcacgg gagactggag
     1201 ctttcgacgc tgctccaatg cggcttgcgg cgtgttctgg ctggatccgg cgccactgga
     1261 aagcgagctg tggaaggcct atgccagcta ccatacccat acgcgcaaat catccaacaa
     1321 actgaccaga gccgcgctca gtctggcgca tcgcgtactc aagttcatcc tgcttccagt
     1381 ctggctcatc aacgggctga agcgcgaggc ggataatctg cgcttcatga cccttgccga
     1441 tgagcctgct ggcaggctgc tggacgtggg ctgcggcgcg ggacgttttc ttaaccggat
     1501 gagaaagcgc ggctgggagg tcgagggtgt ggatttcgat ccccaggccg cacagaaggt
     1561 tgccgcgcgc tatgccatca agacccatgt cggtgatctg gtcacctgtg ctttgcctgc
     1621 cgacagtttc gatgtcgtca cgatgagtca ggcggtggag catctgttcg atccacgcaa
     1681 gaatctgcaa gagtgtttgc gcatcctcaa gccaggcgga ctgctggtga tgaccacgcc
     1741 gaatgtggca agccggggtg ccgcgctgtt cggcgcgttc tggcggggct gggagccgcc
     1801 tcggcatttg cacctgttct cggtcgcatc cctgcgccag ctcaccgaac agtgcggttt
     1861 cgaggttgtc gaggcgggta ccttttctgc aggttctgcg gtggtctatc gcgtgagccg
     1921 ttccaaccag ctggcacatg atggcgaaac agccaacccg ctggccttgc tgttctgggg
     1981 ttatcggcaa gaattgagcg aatatctcga tcagcaatcc gcgtgcgaca ccgggcagaa
     2041 tgtcctgatt cgagcgcgca aacccatgca aggagcggac tgaccatgtt ttctatcatc
     2101 atcccgacct ggaacaatct ggcgctgctg caactgtgca tccgcagtat ccggcaaaat
     2161 tctgcccatg cgcaccagat catcgtgcat gtcaacgacg gcagcgacgg cacgctggcc
     2221 tgggtaagag agcagggctt tgcgcacacc gcctcgccgg agaatatcgg tatctgtctg
     2281 gccgtcaacg aggccgcaat gcacgcgacg cgcgactaca tcctgtatct gaacgacgac
     2341 atgtattgct gtcccggctg ggacagcgcg ctgatcgaca ggctcgggca gctggatacc
     2401 gatctgttca tgctctccgg caccatgatc gagccgcgcg acactaacaa tccctgtgtg
     2461 atcgtgcgcg attacggccg cgaggtggaa agttttgatg aagcagctct gctcgcggaa
     2521 ctgccgcaac atcgcaaagc cgactggtgc ggggcgacct ggccgccgac gctggtgagc
     2581 cgccgctggt ggttcaaggt cggcggctat agcagcgagt tctcacccgg catgagcagc
     2641 gacaacgatt tttcgatgaa actgtggcat gcggggtgcc gcgtgttcct gggcgtgggc
     2701 gattcgctgg tctaccactt tcagtgcaag tccaccggca aggtgaagaa gaacgacggc
     2761 ggcaagcagt tcctgaacaa atggggcgtg cgtcaatcgg tgttcgaccg ctactatctg
     2821 cggcgcggcc agcttgccaa ggggttggcg ctggaggagc ctgaggatac gcgcgaattt
     2881 cgctggcaga tgttccgcag ccggttgaaa cgcgcgctgg gttaacgcgt ggaattcagc
     2941 gcggcgagtt tgcggtattt ccaataggtg gtgcgcgcat tcatgcgggc gatgtcccat
     3001 ccggcaaggc cgtcgaggaa ccccaggcgt aaaaaatagg tgcgcagaaa ggcccagcca
     3061 ccgcgcaacg gcgcatcgac tgacgttgcg cgtttgcccg cccgcaacat ctgctgcgcg
     3121 gcggagtcgg agtagtgcgc caccttgcgc tcgacatcgt ctttggtcag atagctgtag
     3181 tgcagcagcg gactggtcag ttttccggtg gggccttgca gctgtaccga ctcatgcacc
     3241 agatcgtccg agaaccgggc cgcgccgcgc ttgaacaggc gcagcacata atccgggtgc
     3301 cagccggaat gacggatgaa tttgccgcag aactgcgaca gacggggcag gtagaagcca
     3361 tcgtatggcc cctccgtcat cgcgttgatg agttgcgcac gcagctctgg cgtcacgcgc
     3421 tcgtcggcat ccagcgataa tatccagtct ttgctggcat ggtcgagtgc gaggtttttc
     3481 tggatgccga aaccctgcca atcggcatga acaaaaacgt gaggcgtgaa ttcgcggcag
     3541 attgcgacgg tgtcgtcggt gctgcccgag tccactacga tgatctcgtc ggcccatgcc
     3601 accgactcca gacaggcgcg gatgttggca gcctcgttct tggtgatgag gacgacggac
     3661 agcaagggca cgcctaatgc ccgttggatg tcggggtgta ctccggcacc cactctacga
     3721 gtgcgttgcg tacctgatcg tccggcatcg tgcttgcttc ggtccatttg agcagctgtt
     3781 ccagccaggc tgcatcggcc tgtctcgcct gtgcgatgcg cagtttcgga tgcggcgtgg
     3841 gcagcgtgtt ttcgtcgttg gccaacaatt cctcatatag tttttcgccc ggacgcaggc
     3901 cggaaaactc gatctggatg tcgtcttcac tgaagccgga caggcgtatc aggtctttcg
     3961 ccagatcgac gatcttcacg ggctcaccca tgtcgagcac gaatatctcg ccgccacggc
     4021 ccatcagtcc ggcctgcagc accagttgcg ccgcttccgg gatggacatg aagtagcgcg
     4081 tgatctcggg atgggtcacg gtgatcggtc cgccctgcgc gatctgcgcg cggaacttcg
     4141 gaatgacgct gccggacgag cccagtacat tgccgaagcg caccgagatg aagcgcgtgg
     4201 aactactcgg ttgcagcgcc tggcaggcca tctcggccag ccgtttggag gcgcccatca
     4261 cgttggtggg attcaccgcc ttgtcggtgg agatcagcac gaacttgcct acgccatgct
     4321 gttgcgcggc acgggccacg gtgtgggtgc cgagcacatt gttgcgtatc gcctgccagg
     4381 cattgtgctg ttccatcatc ggcacatgct tgtaggcggc ggcgtggaat agcaccgctg
     4441 gttgatgcaa tcgcagcgcc tcggctactc gatccgcatc gcgcacatcg ccgaccagac
     4501 agacgaagtt cagcgttggg aaggtctgtc tcagctcctg ttccagtgta tagagcgcga
     4561 attcgctcaa ttcgaacagc accagtttgg cgggagaaaa ccgcgcgatc tggcggcaga
     4621 gttctgagcc gatggagccg cctgcacccg tgaccattgc gaccttgccg gtcagcaggc
     4681 cgtgcagccc ggcattgtcc agctccaccg gatcgcgccc cagcaggtcg tccagttcga
     4741 tggggcgcag ttgcgatatc gcgaccttgc cgctcatcag atcgtcgaag gacgggacgg
     4801 tcaacgcttt gacgccggcc tgattgcaca gatggatcgc gcgtttgcgc tgctggtgac
     4861 tgctggaggg catcgcgatg atgacgtgtt cgacgttgaa atgctcggcc cagtgcgaca
     4921 gctgatccag cgagcctaag atttttacgt catttagcgt gcgattgtgc ttttctttat
     4981 cgtcgtccag aaagcctact tgttgccagg tagcgctctt ggccagatcc ttggatagca
     5041 gggcggccgc atctcctgcg cccagcacca gtacaggttc ggagttcagc ttgatgctgc
     5101 tgtacagact gtgctccttc atcatgcggt aggcgaaacg tccgccaccc atcagcagga
     5161 tcagcagcag aggatcgata accaataccg agcgagggat gatctcgcca agtcgcagca
     5221 tccagagcaa cagggagatt gcggcagccg agagggcaac gcccagcacg atacggcgca
     5281 ggtcggtggt gctggcatag cgccagaggc cgcggtacag gctgaaacgc cagaagatga
     5341 acagttgcag cggcactatc cagggcaaag tgtgtttgat ttcggcaacc acattgccgg
     5401 gcaagtcgaa attaaagcgc agcagatagg ccatattcca ggccagcgct gctgcaatgg
     5461 cgtcgtggag cacgacgata acaattctgc tgttcgattt acgtaacatc catgctctcc
     5521 agcttgcttc gatgctgtcg ccagctgtgc tctatccaca tcgacaaggc tccatacacg
     5581 atgacccaga aaagcagcag caaggcctga ttctgggcgg ttgagttgat attcagcagg
     5641 gc
//
LOCUS       DASUIV010000214         5113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1074917, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000214 DASUIV010000000
VERSION     DASUIV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074917"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VFW59_07740"
     CDS             <1..171
                     /locus_tag="VFW59_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEX5364147.1"
                     /translation="GNCFECDNCYGVCPDNAVIKLGTGKRFEFNYDYCKGCGICVAEC
                     PCGAIKMVAEDI"
     gene            238..1185
                     /locus_tag="VFW59_07745"
     CDS             238..1185
                     /locus_tag="VFW59_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364148.1"
                     /translation="MKQADYVKTLDWLRANPPFDELCSRYPDEWTAVQDDIAAIIERG
                     VAEELKAYLERFSAQGSSPAQSARSREAALSKFIRSRMAHEAVKKLSLATLAADTGVT
                     KGNKLRFNWFNGFIAQRLLFARQLERKPVSLFWFRLFWPLLWQRKRLMPLVQPRGIYC
                     FYSQELVGELAEMIGTRSCLEIGAGDGTLTRFLKNRGAQVTASDNHGWDNAVDYPDWV
                     VKLDACTALSHYQPEVVICSWPPAQNDFERCVFNTASVQLYIVIGSRHRFAFGNWDDY
                     KRQGAFDRVEDEHLGALLLPPELESAVHVFRRKQSDDHA"
     gene            1187..1519
                     /gene="hypA"
                     /locus_tag="VFW59_07750"
     CDS             1187..1519
                     /gene="hypA"
                     /locus_tag="VFW59_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX5364149.1"
                     /translation="MSLAESVRQIIEEAGQAQGFTRVKTVWLEIGQLSSVEPEAMRFC
                     FEAAMAGSIAAQARLEIVETPGQGRCETCVRDVPIIALHEACPYCGGYQVKVTGGDAM
                     RVKELEVE"
     gene            1519..2382
                     /gene="hypB"
                     /locus_tag="VFW59_07755"
     CDS             1519..2382
                     /gene="hypB"
                     /locus_tag="VFW59_07755"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX5364150.1"
                     /translation="MCMTCGCGIGQTLIGGHALRHSRKEGGALRFRAIEHGKPHTHEH
                     AAMDFGAGQAGSHAPGVTQTRMVKLERDILAKNNDYADENRRYLAAHGIFALNLVSSP
                     GSGKTTLLVKTIAALNAQLPLAVIEGDQQTEYDAERIRAAGAPAIQINTGKGCHLDAQ
                     MVGQAMQRLALPDDSLLLIENVGNLVCPAGFDLGEAHKVVILSVTEGEDKPLKYPDMF
                     RAADLMLLNKCDLLPYLSFDADMAVQYAHRINPGLKVIRTSATSGEGMEEWQEWIADG
                     CRIAIAEKLKV"
     gene            2384..3496
                     /locus_tag="VFW59_07760"
     CDS             2384..3496
                     /locus_tag="VFW59_07760"
                     /inference="COORDINATES: protein motif:HMM:NF036810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX5364151.1"
                     /translation="MAQQDTVIELSCSGPSVLACGAWLKNTVCVTRGDRAYVSQLIGD
                     LDSADTCLMLEQSVARLCAKLDVRPEIVAHDLHPDFFSSRYAQSYAEQHGLPIVAVQH
                     HHAHIAAICAEHHVTEPVLGLALDGVGLGTDGGAWGGELLRVHGAAFQRVGHLAPLAM
                     PGGDRAAREPWRMAAAALYQLGRGAEIAARFPNQSGAQTIETMLQRSLNCPTTSSMGR
                     LFDAAAGLLNVSAVQAFEAQAAIQLQQHAEGHGPVASLNQGYRIGVDGILDFSPLLAE
                     LADCRNADYGAALFHATLAAGLADWVTRTAAEQGVDLVAFGGGCFHNAILLKALSDRL
                     AASGLQVLTAHQIPPNDSAISLGQAWVALQSKKRGS"
     gene            3498..3743
                     /locus_tag="VFW59_07765"
     CDS             3498..3743
                     /locus_tag="VFW59_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX5364152.1"
                     /translation="MCLAIPARIEEMVGADSAIVNLGGMRKEVSLALVEDVAVGDYVI
                     VHVGYALQRLDTEEAERTLAAFAEMGVMNDLADGSGI"
     gene            3740..4855
                     /gene="hypD"
                     /locus_tag="VFW59_07770"
     CDS             3740..4855
                     /gene="hypD"
                     /locus_tag="VFW59_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX5364153.1"
                     /translation="MKYVDEFRDGELAKNIAANIAREARVDRSYRLMEFCGGHTHAIS
                     RYGLADLLPTSVRMIHGPGCPVCVLPIGRVDQAIRLAREHDVILCTYADTLRVPASEN
                     LSLMRAKAAGADVRMIYSTQDALQVARDNPERQVVFLAIGFETTTPPTAVAVKQAAAE
                     GLGNFSILCNHVLTPAAMHAILAVEGGVALDGFVGPAHVSTVIGSQPYEIFARDYGKP
                     VVIAGFEPLDVMQAVLMLVRQINEGRSEVENEFTRAVTRDGNRKAQALVDEVFELRDT
                     FEWRGLGSVPHSALKLRPAFSAFDAEARFGVDYVAVPDNKACECAAILRGQKRPQECK
                     IFGTVCTPANPVGSCMVSSEGACAAHYSYGRFREEGV"
     gene            4852..>5113
                     /locus_tag="VFW59_07775"
     CDS             4852..>5113
                     /locus_tag="VFW59_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX5364154.1"
                     /translation="MSTVKPNYTRALDWKTGRVDMSYGSGGRAMAQLISELFAAEFDN
                     PYLGQGNDGALLPPANGRLVMSCDSHVVSPLFFAGGDIGCLSV"
BASE COUNT         1045 a         1431 c         1645 g          992 t
ORIGIN      
        1 ggcaactgtt tcgaatgcga caactgttac ggcgtatgtc cggataacgc ggtgatcaag
       61 ctcggaaccg gtaaacgctt cgagttcaat tacgactatt gcaagggttg cggcatttgc
      121 gtggcggagt gtccctgcgg ggcgatcaag atggtggcgg aggatatctg aacaaaggat
      181 gattcagatt caacggcaac atagaatgcc gaccaccgcc atgttgaata aattttcatg
      241 aagcaagccg attacgtaaa aacgctggat tggttaagag ccaatccccc tttcgatgag
      301 ctgtgttcca ggtatcccga cgaatggacg gcagtgcaag acgatattgc cgccattatc
      361 gagcgcgggg tggccgaaga gctgaaagcc taccttgagc gtttctccgc tcagggatca
      421 tcgcccgctc aaagcgcacg atcacgggag gctgccttat cgaaattcat acgcagccgc
      481 atggcgcacg aggccgtcaa gaaactcagc ctggccacgc tggctgcgga taccggagtg
      541 acgaagggta ataagctgcg cttcaactgg ttcaacggtt ttatcgccca gcgtctgctg
      601 ttcgccagac aattggagag aaagccggtt tccctgttct ggttccggct attctggccg
      661 ctgttatggc agagaaaacg cctgatgccg ctggtgcaac ccagaggcat ttattgtttc
      721 tattcgcaag agctggtcgg agagctggca gaaatgatcg gcaccagaag ctgtctggag
      781 atcggtgcgg gcgacgggac attgacgcgc ttcctgaaga atcgaggcgc acaggttacg
      841 gcaagcgaca atcacggctg ggacaatgcg gtggactatc cggactgggt cgttaaactc
      901 gatgcctgta cggcactgag tcactaccaa ccggaggtgg tcatctgttc ctggcctccg
      961 gcgcagaatg atttcgagcg ctgtgtgttc aatacggcca gtgtgcagtt atatatcgtc
     1021 atcggcagcc gccatcgttt cgcgttcggt aactgggacg attacaaacg gcaaggtgct
     1081 ttcgataggg tggaggacga gcatctgggt gcgctgctgt tgccgccgga actggaatct
     1141 gccgtccatg tattccgacg caagcaaagt gacgatcatg catgagatgt cattagccga
     1201 aagcgtgcgc cagatcatcg aagaggcggg gcaggcgcaa gggtttaccc gagtcaagac
     1261 cgtgtggctg gaaattggcc aactctccag tgtggaacct gaagccatgc gtttctgctt
     1321 tgaggcggcg atggcaggca gcatcgccgc acaagccaga ctggaaatcg tcgaaacgcc
     1381 gggacaggga cgatgtgaaa cctgtgtccg cgatgtgccg atcattgcgc tgcatgaagc
     1441 ctgtccatat tgcggaggct atcaggtaaa ggtgaccggt ggcgatgcaa tgcgagtcaa
     1501 agaactggag gtcgagtgat gtgcatgacc tgcggctgtg gaatcggcca gacattgatc
     1561 ggtggacatg ccttgcgcca ctctcgcaag gagggtgggg cattgcgctt tcgtgccatt
     1621 gaacacggca agccgcacac gcatgaacac gcagcgatgg acttcggtgc agggcaggcg
     1681 ggttcgcatg caccaggggt gacacagacg cgcatggtga agctggagcg cgacatcctc
     1741 gccaagaata acgactatgc ggatgagaac cggcgctatc tcgccgcgca cggcatcttc
     1801 gcactcaacc tggtctccag ccccggttcc ggcaagacca cgttgctggt aaaaacgatc
     1861 gccgcactga acgcccagct gccgctggcc gtgatcgaag gcgaccagca gaccgaatat
     1921 gatgcggaac gcatccgcgc cgccggtgca cccgccatcc agatcaacac cggcaagggc
     1981 tgccatctgg atgcccagat ggtcggccaa gccatgcaac ggctggcttt gccggacgac
     2041 agtctgctgt tgatcgaaaa tgtcggcaat ctggtgtgcc cggccggatt cgatctgggc
     2101 gaagcacaca aggtcgtcat cctgtcggtc accgaggggg aggacaagcc gctgaagtat
     2161 ccggacatgt tccgcgccgc cgacctgatg ctgctgaaca agtgcgactt gctgccatac
     2221 ctgagtttcg atgccgatat ggctgtccaa tatgcgcacc gcatcaatcc cggactcaag
     2281 gtgatccgta cctcggcgac cagcggtgaa ggcatggaag aatggcaaga gtggatcgca
     2341 gacggctgcc gtatcgccat cgccgaaaaa ctgaaagtct gaattggctc aacaggatac
     2401 cgtcatcgaa ctgtcatgct ccgggccatc tgtgctggcc tgcggtgcct ggttgaagaa
     2461 cacggtatgc gtgacgcgcg gcgatcgggc ctatgtctcg caactgatcg gcgatctgga
     2521 tagcgccgat acctgcctca tgctggagca gagcgtggct cgcctgtgtg ccaaactcga
     2581 tgtcaggccc gagatcgtcg cacacgatct gcatcctgat tttttcagca gccgctacgc
     2641 gcagtcctac gccgagcagc acggcctgcc catcgttgcg gtgcaacacc accacgccca
     2701 catcgccgcc atctgcgccg aacaccacgt caccgaaccc gtgctcgggc tggcgctgga
     2761 cggcgtggga ctgggcacag atggcggtgc atggggcggt gagttgctgc gggtgcacgg
     2821 ggcggcattt cagcgtgtcg ggcatcttgc accattggcg atgccgggag gcgaccgcgc
     2881 cgcgcgcgaa ccttggcgca tggcggctgc ggcgctgtat caactggggc ggggcgccga
     2941 gatagccgcg cgtttcccga accagtccgg cgcacagacg atcgaaacga tgttgcagcg
     3001 aagtctgaac tgtccgacga cttccagcat gggcaggctg ttcgatgcgg cggcgggctt
     3061 gttgaatgtg agcgcagttc aggcattcga agcacaggca gccattcagc ttcagcaaca
     3121 tgcggaaggg catgggccgg tcgcctcgct gaatcaaggc taccggatcg gcgttgatgg
     3181 catcctggat ttcagccctc tgcttgcgga actggccgat tgccgaaacg cggactatgg
     3241 cgcggcattg ttccatgcca ccctcgcagc gggactggca gactgggtaa cgcgcacagc
     3301 ggcagagcag ggcgtcgatc tggtcgcatt cggtggaggc tgttttcata acgctatcct
     3361 gttgaaagcg ttgtctgata ggcttgccgc gtcaggctta caggtcctga cggcgcatca
     3421 aataccgccc aacgacagcg cgatctcgct ggggcaagca tgggtcgcac tgcaatctaa
     3481 gaaacgagga agctgaaatg tgtctcgcca tacccgcacg tatcgaagaa atggtcggtg
     3541 cagatagcgc catcgtcaat cttggcggca tgcgcaagga ggtctcgctc gcgctggtag
     3601 aggatgtcgc cgtcggcgac tacgtcatcg tacatgtcgg ttatgcgctg caacgccttg
     3661 ataccgaaga ggccgagcgc acgctggcgg cctttgccga gatgggggtg atgaatgact
     3721 tggccgatgg ttcgggcata tgaagtacgt cgacgaattc cgcgacggcg aactggcaaa
     3781 gaacatcgcc gccaacattg cccgtgaggc cagggtcgac cgcagttacc ggctgatgga
     3841 gttttgcggc ggccataccc acgccatttc gcgttatggg ctggcggact tgttgccgac
     3901 cagcgtgcgg atgatccatg gcccgggttg cccggtgtgc gtgctgccga tagggcgggt
     3961 ggatcaggcg atccggctgg cgcgcgagca cgacgtgatc ctgtgcacct atgccgacac
     4021 gctgcgcgtt ccggcctccg aaaacctctc gctgatgcgc gccaaggcgg cgggggcgga
     4081 cgtgcgcatg atctattcga cgcaggatgc gctgcaggtc gcgcgcgaca atccggaacg
     4141 gcaggtggtc tttctcgcca tcggtttcga aaccaccacg ccgccgactg cggtcgcggt
     4201 caaacaggcg gctgcggagg ggctgggcaa cttcagcatc ctgtgcaatc acgtgctgac
     4261 cccggcggcg atgcatgcga ttctggccgt cgaaggaggt gtcgcgctgg acggtttcgt
     4321 cggcccggcg catgtctcga cggtgatcgg cagccagccc tatgagatat tcgcccgcga
     4381 ctacggcaag ccggtggtga tcgcggggtt cgagccgctg gatgtaatgc aagccgtcct
     4441 gatgctggtg cgccagatca acgaggggcg ctcggaagtc gaaaacgagt tcacccgtgc
     4501 agtgacccga gacggcaacc gcaaggcaca ggcactggtc gacgaggtat tcgaactgcg
     4561 cgatacgttc gagtggcgcg gtctgggcag tgtgccgcac agtgcgctga agctgcgccc
     4621 ggcattctcc gccttcgatg ccgaagccag gtttggcgtc gattatgtcg cggtgccgga
     4681 caacaaggcc tgcgaatgcg cggcgattct gcgcggccag aaacgtccgc aggagtgcaa
     4741 gatattcggc acggtctgca ccccggccaa tccggtcggc tcctgcatgg tgtcttccga
     4801 aggcgcatgt gcggcacatt acagttacgg acggttccgg gaggaaggcg tatgagcacg
     4861 gttaaaccca attacacccg cgcgctggac tggaagaccg ggcgcgtgga catgtcttat
     4921 ggttccggcg gacgagcgat ggcgcagctg atctcggaac tgtttgccgc agaattcgac
     4981 aatccctatc tggggcaggg caatgacggc gcattgctgc ctccggccaa cggacggctg
     5041 gttatgtcct gcgacagcca tgtggtgtcg ccgctgttct tcgcgggcgg cgatatcggc
     5101 tgtctgtcgg tgc
//
LOCUS       DASUIV010000215         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1353481, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000215 DASUIV010000000
VERSION     DASUIV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353481"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /gene="arsB"
                     /locus_tag="VFW59_07780"
     CDS             complement(<1..918)
                     /gene="arsB"
                     /locus_tag="VFW59_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX5364155.1"
                     /translation="MNLFERYLTLWVALCIMAGVVLGQFLPGVFQAIASLEVAKVNIP
                     VGVLVWVMIVPMLLRIDFGALHQVKAHARGIGVTLFVNWAVKPFSMAFLGWLFIRHVF
                     ADYLPQDQLDSYIAGLILLAAAPCTAMVFVWSNLVKGDPYFTLSQVALNDTIMIFAFA
                     PLVALLLGVASIAVPWDTLFVSVVFYIVIPVIVAQLLRRRLLVNGEDGFNHAMARIAP
                     FSMGALLVTLVLLFALQGNAIVAQPLVIALLAVPILIQVLFNSGLAYWLNRRLGVVHC
                     VAAPSALIGASNFFELAVATAISLFGFQSG"
     gene            complement(928..2682)
                     /gene="arsA"
                     /locus_tag="VFW59_07785"
     CDS             complement(928..2682)
                     /gene="arsA"
                     /locus_tag="VFW59_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HEX5364156.1"
                     /translation="MKFLEQPPRFLFFTGKGGVGKTSIACATSIQLAEAGKRVLLVST
                     DPASNVGQVFGVSIGNRITAIPAVPQLAALEIDPQAAAQVYRDRIVGPVRGVLPDAVV
                     KSIEEQLSGACTTEIAAFDEFTALLIDSALTREYDHIIFDTAPTGHTIRLLQLPGAWS
                     GFLDEGKGDASCLGPLAGLEKQREQYKAAVDALADGSRTRLILVARAQQATLREVART
                     HEELAAIGLKQQYLVINGILPHAETANDPLAAAIHEREQTALGAIPEVLKALPLDQVM
                     LKPFNLVGLEALRHLLVVTPPQSQINVAAPIELDEPGLSELVDGIAADGHGLVMLMGK
                     GGVGKTTLAAAIAVELAHRGLPVHLTTSDPAAHLTETLSGTLDNLTVSRIDPHAETER
                     YRQHVLEMKGSQLDAQGRALLEEDLRSPCTEEIAVFQAFSRIIREAGKKFVVMDTAPT
                     GHTLLLLDATGAYHREVTRQMGDKGLHFTTPMMQLQDSKQTKVLIATLAENTPVLEAA
                     NLQTDLRRAGIEPWAWIINTSLAAASAQSPLLRQRAANELREINAVASQYAQRYAVVP
                     LLRNEPVGVTRLLELAAG"
     gene            complement(2756..3370)
                     /gene="arsD"
                     /locus_tag="VFW59_07790"
     CDS             complement(2756..3370)
                     /gene="arsD"
                     /locus_tag="VFW59_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HEX5364157.1"
                     /translation="MKKLQIFDPALCCSTGVCGVDVDQALVGFSADVDWARQNGAQIE
                     RFNLAQQPLAFAENAAVKVFLETSGQEALPLALLDGEVVLSGRYPSRDELAGWFGIAT
                     APSLFTAQVAELVAIGAAIACNCEPCFKYHYDQARKLGVSDLDMRYAVDLAQKVKDTP
                     SRVMLALAERYLGPSTSSTTGKANSCCVTEPVAIVQPASKKCCG"
     gene            complement(3381..>3794)
                     /locus_tag="VFW59_07795"
     CDS             complement(3381..>3794)
                     /locus_tag="VFW59_07795"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX5364158.1"
                     /translation="LGKGRFKAYSAGSKPAGRVNPGALELLQQQGYSIEGLRSKSWDE
                     FAVPGAPEFDFIFTVCDNAAGETCPLWLGHPATAHWGIPDPAHVEGDEARRAAFKKAY
                     AQLSRRIQLFMNLPIEKLDKLALKEKLTEIGRIQD"
BASE COUNT          803 a         1171 c         1093 g          727 t
ORIGIN      
        1 gcccgactgg aagccgaaca ggctgatcgc ggtggcgacg gccagttcga aaaaattgct
       61 cgcgccgatc agcgccgacg gcgcggcgac gcaatgcacc acgcccagcc gacggttcag
      121 ccagtaggcc aggccggagt tgaacagcac ctggatcagg ataggcacgg ccagcagcgc
      181 gatcaccagc ggctgcgcga cgatggcatt gccctgcaac gcgaacagca gcaccagcgt
      241 caccagcagc gcgcccatcg agaacggcgc gatgcgcgcc atcgcatgat tgaacccgtc
      301 ctcgccgttc accagcaacc tgcggcgcaa taactgcgcg acgatcaccg gaatcacgat
      361 gtagaacacc accgacacga acagcgtgtc ccacggcacg gcgatgctgg cgaccccgag
      421 cagcagcgcc accagcggcg cgaacgcgaa aatcatgatg gtgtcgttga gcgcgacctg
      481 cgacaacgtg aaatacggat cgcccttcac cagattgctc cacacgaaca ccatcgcggt
      541 acagggtgcg gcggccagca ggatcagccc ggcgatatag ctgtcgagct ggtcctgagg
      601 caggtagtcg gcgaacacat ggcgaatgaa cagccagccg aggaaagcca tcgagaacgg
      661 tttcaccgcc cagttgacga acagcgtgac gccgatgccg cgcgcatgcg ccttcacctg
      721 atgcaacgcg ccgaagtcga tgcgcagcag catcggcacg atcatcaccc acaccaatac
      781 gccgacggga atgttcacct tggcgacttc caggctggcg atggcctgaa atacgccggg
      841 aaggaattgc ccgagcacga ccccggccat gatgcacagc gcgacccaga gggtcagata
      901 acgttcgaac agattcatgg cattgtccta tccggcagcc agttcaagca ggcgcgttac
      961 acccacaggc tcattcctga gcagcgggac aactgcataa cgttgcgcat actgactggc
     1021 gacagcattg atttcgcgaa gctcattcgc ggctctttgc cgcagcagcg gcgattgggc
     1081 ggaagctgcc gccaaacttg tgttgatgat ccatgcccaa ggctcgatgc ctgctcgacg
     1141 caagtccgtt tgcaaattgg cggcttcgag taccggcgtg ttttctgcca atgtcgcaat
     1201 cagcaccttg gtctgtttgg agtcctgcaa ttgcatcatc ggggtggtga agtgcagtcc
     1261 cttgtcgccc atctggcgtg tgacttcgcg gtgataagcc ccggtcgcat cgagcagcaa
     1321 caacgtgtgg cccgtcggag ccgtgtccat caccacaaac ttcttgcccg cctcgcgaat
     1381 gatgcgcgag aaagcctgga ataccgcgat ttcctcggtg cagggcgagc gcaaatcctc
     1441 ttccagcaga gcgcggcctt gcgcatcgag ttgagagccc ttcatttcca gcacatgctg
     1501 acgatagcgt tcggtctcgg catgaggatc gatgcggctc accgtcaggt tatcgagagt
     1561 cccgctgagc gtttccgtca gatgggcagc cgggtcggat gtggtgagat gcacaggcaa
     1621 gccgcgatgc gccagctcta cagcgatagc agccgccaag gtagtttttc cgacaccgcc
     1681 cttacccatc agcatcacca ggccatgtcc atccgctgcg atgccatcca ccaattccga
     1741 aaggccgggt tcatccagtt caatcggtgc agccacgttg atttgagact gcggcggtgt
     1801 gactaccagc aaatggcgca gcgcttccaa cccgaccagg ttgaagggct tgagcatgac
     1861 ttgatcgaga ggcagcgctt tcaggacttc gggaatagcg ccgagcgccg tttgttcgcg
     1921 ctcgtgaata gccgcagcca acggatcatt tgctgtttcc gcatgaggca gaatgccgtt
     1981 tatcaccaga tactgctgct tcaacccgat ggcagcgagt tcttcatgcg tgcgcgcaac
     2041 ttcgcgcaag gtcgcctgct gagcgcgagc gaccagaatc agccgcgtgc ggctgccatc
     2101 ggccagcgca tcaacggcgg ccttgtattg ttcccgctgt ttttcaagac cggccaatgg
     2161 gccgaggcat gatgcatcgc ctttaccctc atccagaaag ccgctccatg caccgggcag
     2221 ttgcaacaac cggatcgtgt gcccggttgg cgcagtatcg aaaatgatgt gatcgtattc
     2281 tctggtcagt gcggaatcga tcaacagtgc ggtgaactcg tcgaatgcgg cgatttccgt
     2341 ggtgcatgcg ccggagagtt gctcctcgat gctttttacc accgcatcgg gcaacacgcc
     2401 gcgcaccgga ccgacgatgc gatcacgata gacctgcgcc gctgcctgcg ggtctatctc
     2461 cagcgcagcc agttggggta cggcaggaat ggcggtgatg cgattgccga tggagacacc
     2521 aaatacctgc ccgacgttgg aggcaggatc ggtgctgacc agcagcacgc gctttcccgc
     2581 ttccgccaac tggattgatg tggcgcaagc gatggaggtc ttgccgacgc cacctttgcc
     2641 ggtgaagaac aggaaacgcg gcggttgttc gaggaacttc atgggcgact cctaattgtc
     2701 gaagtacggt atgtagcgat gggtatcgct ttgctcaacc catcctacgg cgcaattatc
     2761 cgcagcactt tttggaagca ggttggacga tagccactgg ttccgtcaca cagcatgaat
     2821 tcgctttccc ggtggtgctt gatgtggagg gacccagata acgttcggcg agagccagca
     2881 tgacgcgcga cggcgtgtcc ttcaccttct gtgccagatc gaccgcatag cgcatatcca
     2941 gatcgctcac gccgagtttg cgcgcctgat cgtagtggta tttgaaacaa ggctcgcaat
     3001 tgcaggcaat ggccgcgccg atggcaacga gttccgcaac ctgtgccgta aacagcgagg
     3061 gcgcagtcgc gatcccgaac cacccggcca gttcatcgcg gctagggtag cgaccggaca
     3121 gaacaacctc tccgtcgagc aatgccagcg gcagcgcttc ctgccccgat gtttcaagaa
     3181 aaactttcac cgccgcattc tccgcaaacg ccaacggttg ctgggcgaga ttgaagcgct
     3241 cgatctgcgc cccgttctgc ctagcccagt ccacgtcagc cgaaaagccg accaaagcct
     3301 gatcgacatc gacaccgcac acgcccgtac tgcaacacag cgcaggatca aaaatttgca
     3361 gtttcttcat cacattctcc ttagtcttga atacgcccga tttcagtgag tttctctttc
     3421 aatgcaagtt tgtccaattt ctcaatcggt aagttcataa acaactgaat ccggcgcgac
     3481 agttgggcgt aggccttttt gaacgcggcg cgccgggcct cgtcgccctc gacatgcgcc
     3541 gggtcgggga tgccccaatg cgcagtagcc ggatgcccca gccacagcgg acaggtctcg
     3601 cccgccgcgt tgtcgcacac ggtgaagatg aaatcgaatt ccggtgcacc gggaacggcg
     3661 aactcgtccc agcttttgct gcgcagtccc tcgatgctgt aaccctgttg ctgcaatagc
     3721 tccagtgccc caggattgac gcgtccggcc ggcttgctgc ccgcgctgta ggccttgaac
     3781 ctgcctttgc ccag
//
LOCUS       DASUIV010000216        16465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_518602, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000216 DASUIV010000000
VERSION     DASUIV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 16465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16465
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518602"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..628
                     /gene="fliS"
                     /locus_tag="VFW59_07800"
     CDS             182..628
                     /gene="fliS"
                     /locus_tag="VFW59_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEX5364159.1"
                     /translation="MNATTAISAYQRVGVESGVAAADPHKLISMLYQGALLAIANAKS
                     SIARKDIPAKGKAISHAITIIDEGLNASLDKKVGGELAQNLSSLYEYMGSRLLTANIK
                     NDVAALDEVARLLTDLKSAWDDIRPTVTPAANATVESAPKPIYARG"
     gene            635..958
                     /locus_tag="VFW59_07805"
     CDS             635..958
                     /locus_tag="VFW59_07805"
                     /inference="COORDINATES: protein motif:HMM:NF017237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HEX5364160.1"
                     /translation="MGIPQVIANYESLSVLTGQMSEAARQGEWDRLIDLEQQCSRQVS
                     SMKPIDAAVALDEPARQLKIRLIKDILSHDAEIRNRTQTWMVQLQRILQSNQQEQKLH
                     RAYGG"
     gene            1064..2089
                     /locus_tag="VFW59_07810"
     CDS             1064..2089
                     /locus_tag="VFW59_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX5364161.1"
                     /translation="MLPANVVNALQTLANSAKPPLALDNNVPAAISKLETGQQLQASV
                     LSKLGEGLFKVQLAGQTMQMRLPGNIQSGDTIKLQVVSVQPRITFSMMASTNPLSTPD
                     QIGDTARLLSNLTDRPIERPLVQQMGSKAVWAATLQAPDVRQLATGLHDALSKSGLFY
                     ESHQAQWVRGERSINQLLEEPQNLLTGKSLTLSPGDTQQSAQDKLAAAQPSQPSAKSD
                     NAPLIAKELQPLVQQQLHTLENHHMVWMGQVWPEQQMQWEIKGEPKHQGRQQDERQWS
                     TEMELSLPKLGDVRARLVFAESGLRLTLHAADAATTALFNRSLPQLKNSLDNAGIALT
                     AAIVEKP"
     gene            2086..2391
                     /locus_tag="VFW59_07815"
     CDS             2086..2391
                     /locus_tag="VFW59_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX5364162.1"
                     /translation="MSDAKPSAQQVAIALAYGQGNSGAPKVVAKGRGLVAQAIIERAK
                     QNGVFVHESEDLVGLLMKVELDQEIPPQLYMAVAELLAWLYRLEHADAPVPPPFMKL"
     gene            2429..3229
                     /locus_tag="VFW59_07820"
     CDS             2429..3229
                     /locus_tag="VFW59_07820"
                     /inference="COORDINATES: protein motif:HMM:NF018967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEX5364163.1"
                     /translation="MKKDIPLTIETITEEDEQECLVTSARQIKSILRTIADAGTNSAL
                     YYTPNHNFIMTSVLDVDEDGLWIEQGSTATVNYLITESKKLTLVSSHAQVKVQFTVDV
                     AASVSYDGYPALFLPLPSKLYRLQRREFFRLSLPPNEQLNCIINVAKAERRSEASPPV
                     DLAVSDLEIPAADISGGGIGLICRQDDVELQPGETYSGCQITLPGAGPIFVNFTVMNL
                     VILSTTRSGKTIKRAGCEFTNLDSQTTAKLQRFITDMQRNKAANSLLV"
     gene            3447..4223
                     /locus_tag="VFW59_07825"
     CDS             3447..4223
                     /locus_tag="VFW59_07825"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HEX5364164.1"
                     /translation="MKKDIPLTIESITDADEQECRITSSRQIASILRNIAETGSNAAL
                     YYNSKRDFIMTTILDVDEDGMWVEQASSATVNHQIEESKRIKLVSSHNQVKVQFGADG
                     ASSVTYDERPAFFIPIPDSIYRFQRREYFRLSLLPSEQMRCIINMARPEAPGTIVQIE
                     VPVADISGGGIGLTCMEGEVDINAGESYSKCQINLPGVGPVLVNLIVRNLVPVSTTKS
                     GKTISRAGCEFKDLDGQTTVKLQRFITDKQRLMAANATSL"
     gene            complement(4237..4563)
                     /gene="fliE"
                     /locus_tag="VFW59_07830"
     CDS             complement(4237..4563)
                     /gene="fliE"
                     /locus_tag="VFW59_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX5364165.1"
                     /translation="MNTSAVDSLLAQMRVAKAAATGMREPSQVATQPGKVDFSDALKS
                     ALDGVAQAQSKSEDMRKAFVLGDERVSLSDTMIAMQKSSINFQTAIQVRNRVVQAYND
                     IMNMQV"
     gene            4730..6370
                     /gene="fliF"
                     /locus_tag="VFW59_07835"
     CDS             4730..6370
                     /gene="fliF"
                     /locus_tag="VFW59_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX5364166.1"
                     /translation="MAEQADNVATNRFNRLTLQQKLGLGAGVAALFALIAGAWIWGQT
                     PEYRVLYSNLSDRDGGAIIASLQQMNIPYKFAEGGGALLVTSNQVHEARLKLASQGLP
                     KGGNVGFELMENQRFGITQFAEQVNYQRALEGELARSVESIGAVAAARVHLAIPKPSV
                     FVKEHQQPSASVVLSLQGGRLLDSAQVSAIIHLISSSVPEMSAKNVTVVDQNGTLLSA
                     DHNGNVNEGLDANQLKYVQQIEHSYIQRIEMLLGPMLGANNLRAQVSADIDFSRTEQT
                     SEVFKPNQPPNESAVRSQQISETLNGNGLTASGVPGALSNQPPAPATAPIVAASPAVG
                     VNAGTDLAGTDLNVSNLQKESTINYEVDRTIRHTILPVGAIKRLSVALVVNGNRKVVD
                     AKGKSVTKPFTDAEKEQINNLVKEAMGFDQNRGDSLNVQIASFADTTENIEETPLWKQ
                     PDAIELGKELLKYALIAAIMLFVVFRIIKPAFQSLINPASETGGFSAAPEIVEPEEAG
                     ATYKPTSPGFESTLMAAKQLAMQDPKIVASVIKEWVEQ"
     gene            6367..7359
                     /gene="fliG"
                     /locus_tag="VFW59_07840"
     CDS             6367..7359
                     /gene="fliG"
                     /locus_tag="VFW59_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX5364167.1"
                     /translation="MINGIDRSAIFLMSLGETEAAAVLKYLEPKEVQKISAAMMKLKN
                     LSREDIAGVFNDFMISAAGKTDIGLDSNEYIRNMLIKALGDDKAAGLLDRIMHNSDTS
                     GIESLKWMDPGAVAEMIGNEHPQIIATILVHLEPDLAAEIMKMFAERTRNDVLLRIST
                     LDGVQPLALRELNDVLSKLLSGGGTGKKTLHGGVETAAEILNFMGHTLEAEMMENVRS
                     YDPELAQKIEDKMFVFENVLEIDDRGIQLILREVQSESLIVSLKGASQELREKIFKNM
                     SSRASEMMREDLEAKGPVKLSEVEANQKEILKIVRRLADEGQISLGGKSEEAYV"
     gene            7352..8005
                     /locus_tag="VFW59_07845"
     CDS             7352..8005
                     /locus_tag="VFW59_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEX5364168.1"
                     /translation="MSSVLIPKEELTAFQRWELASFDPPPPPKEHVLTPEEEQAEIER
                     VTAAELERIRQQAYDEGYAQGQEAGYASGSAQIQAEIGQLQTAMQELHTALNQVDTEL
                     AQSLLDLSLEIARKMVGEALKIKPEFILNTVSSAIGNLPHFNQHAHLVLHPEDVELVR
                     KHMGDNLAHGGWKLIGDSSIKRGGCRVETTHTKVDATVEARWKRIVESIGEDESWLA"
     gene            7993..9408
                     /gene="fliI"
                     /locus_tag="VFW59_07850"
     CDS             7993..9408
                     /gene="fliI"
                     /locus_tag="VFW59_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX5364169.1"
                     /translation="MASLNPSSHSAKWRGLLDESREHIAQCQTMPASGHLTKVTGLVM
                     EAVGLKMSVGSTCLIHLPSGVVAAEVVGFSGEKLFLMPENDVYGLVPGARVTPIDSIH
                     IPTLGSKPPLPELRASDSAKQLPVGDMLLGRVVDGAGRPLDQFGPLNNTSFAPLQSRP
                     FNPLERARIDTVLDVGVRAINSLLTVGRGQRMGLFAGSGVGKSVLLGMMARYTNADIT
                     VVGLIGERGREVKEFINDILGEEGLARSVIVATPADTSPLMRLQGAAYATTIAEYFRD
                     QGKNVLLIMDSLTRYAMAQREIALAVGEPPATKGYPPSVFAKLPQLVERAGNGLEGSG
                     SITAFYTVLTEGDDQQDPIADSARAILDGHIVLSRRLVEQGHYPAIDIEASISRVMSH
                     LVSPQHQALVQRFKHLYAHYQRNRDLISVGAYVRGSDPLLDEAILLYPRMAQFLKQGM
                     HDCETYQASAAQLSALFEPVH"
     gene            9420..9896
                     /gene="fliJ"
                     /locus_tag="VFW59_07855"
     CDS             9420..9896
                     /gene="fliJ"
                     /locus_tag="VFW59_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX5364170.1"
                     /translation="MATPFSLTTLLNLAEHHSDSATRKLGQLNQQEYSAQQQLEILLE
                     FRRDYQAQMQKDTEAGMSPAALRNFQQFIYKLDEAIAQQRRQLENSKASTQLGRDEFD
                     VTQRKLKSLDTLRQRHIETQKQIANKAEQKELDEHTGRMAARRMSDELNQAAENEK"
     gene            9927..11057
                     /locus_tag="VFW59_07860"
     CDS             9927..11057
                     /locus_tag="VFW59_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX5364171.1"
                     /translation="MTPVQISPAPQPSGNAKASSQTDSATNNQTADGQPAEPFAKVLS
                     RQVDKSDSPAQKTDAADAGKTDATPQTAAPAVVQPDALAAIMSQIPVEMRGANVTDRA
                     ALISTEGRLPAGIDTALPKIDAALSQIDARTQGKATTSPQADLSAADAHKKTPAGSRN
                     GQDKDFASTLKAETKSLAADTDAMHKSMLGRTDITSDASQLVASQTSAASTISQATLA
                     AMTGTSIGNQPAGPAQTIATPLGSAAWGDDFSQKVSWMITQKNQVAELHLNPPNLGPL
                     DVVIKITDNQATALFTSPHAMVRDAVESALPKLRELLADNGIMLGNATVGDQSPRERE
                     AGEFSGRTTGTSAQRDTDTGLTEPTVPVAAARRHNGVLDTFA"
     gene            11152..11739
                     /locus_tag="VFW59_07865"
     CDS             11152..11739
                     /locus_tag="VFW59_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX5364172.1"
                     /translation="MAKGTKPGAKPEEQEEVAPPQKSKKKLIIIIVAAVLVIGAAAVA
                     AVLLLKPAHPRQSETEETTSEHKVEDSKFPAKFIDVGTFTTNLAPDEGDRFVQTAITF
                     KISKPELEEKISATRPELQHRINMLLQSKLPSELAASEGKTQLAAQIKLQAEEVLGLR
                     KATPTAGAEPAAGGPVAAKPQAGLDEVLFTSFLIQ"
     gene            11764..12753
                     /gene="fliM"
                     /locus_tag="VFW59_07870"
     CDS             11764..12753
                     /gene="fliM"
                     /locus_tag="VFW59_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX5364173.1"
                     /translation="MSEFLSQDEVDSLLKGVTGDTDEVNEDEASAPGTVRSYNLASQE
                     RIVRGRMPTMEVINERFARLFRIGLFNFIRRTPEISVGPVRVLKFGEFIRNLHVPTNL
                     NIIQAKPLRGNGLFIFDPNLVFLVVDNMFGGDGRFHTRVEGREFTQTEQRIIQNLLLV
                     VFETYGKAWEAVHPLEFEFVRSEMNPQFANIATPNEVVIVTTFDIELGGNGGSFHVCM
                     PYSMLEPVKDLLYSTMQGEHLAMDHRWLQLLSKQVQSAQIELIASLGSARITMEQVLR
                     MRSGDIIPLSVNDNITATVDGIPVMECKYGSFNNQYALRVEKMLSTSEGEGNA"
     gene            12806..13213
                     /gene="fliN"
                     /locus_tag="VFW59_07875"
     CDS             12806..13213
                     /gene="fliN"
                     /locus_tag="VFW59_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX5364174.1"
                     /translation="MAEQTTTATAEDDWGAALAEQTAAEKAPAEPAKPHVFEKFGSGG
                     SPTSQNDLDMILDIPVNLTVELGRTKMPIKNLLQLAQGSVVELAGMAGEPLDVLINGF
                     LIAQGEVVVVNDKLGIRLTDIITPAERLRRLNR"
     gene            13215..13691
                     /gene="fliO"
                     /locus_tag="VFW59_07880"
     CDS             13215..13691
                     /gene="fliO"
                     /locus_tag="VFW59_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX5364175.1"
                     /translation="MPVQFRYAFAAFSLAFAQLTAAVEGTRPGYVPPPPAVSSGSIVQ
                     IIFSLLLVLAVVVLVGWLLRRMNLSQQGTGSQLKMIGGIAIGQRERIVLIEIDDTWLV
                     VGVGPGQIRTLHTLPKPEGDTATSRAGQPDQTEGKFAALLSSMLNQRPSAKKSDAP"
     gene            13681..14430
                     /gene="fliP"
                     /locus_tag="VFW59_07885"
     CDS             13681..14430
                     /gene="fliP"
                     /locus_tag="VFW59_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028727.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX5364176.1"
                     /translation="MRPSSLAILAVIALTALAQPAWAEMGIPALTSSPAPGGGQTYSL
                     SLQTLLVLTSLSFLPAIMLMMTGFTRIIIVLSLLRSALGTPSSPPNQVLIGLSLFLTF
                     FVMSPVFDKMYIDAYLPYSENKMDFTQAVDKASIPLKTFMLRQTREADLALFVRISNS
                     EPLQSADQVPLRILVPSYVISELKTAFQIGFVIFIPFLIIDMVVASVLMSMGMMMLSP
                     SIISLPFKIMLFVLADGWNLIIGSLAQSFYT"
     gene            14446..14715
                     /gene="fliQ"
                     /locus_tag="VFW59_07890"
     CDS             14446..14715
                     /gene="fliQ"
                     /locus_tag="VFW59_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX5364177.1"
                     /translation="MTPETVITIGEQAVEMTLMVSAPLLLTALVIGLVVSIFQAATQI
                     NEMTLSFIPKMLGIFVVLIFTGPWMISMLVDYIQRLYGNIPWMIG"
     gene            14737..15513
                     /gene="fliR"
                     /locus_tag="VFW59_07895"
     CDS             14737..15513
                     /gene="fliR"
                     /locus_tag="VFW59_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX5364178.1"
                     /translation="MISITSAQLDAWLATLLFPLVRILAMISTAPVLGNKQVPKRVKI
                     GLSALLAILIAPTLSSIPLVPVGSPQGLLIIIQQIIIGVAMGFTMRMVFTAVEMAGEL
                     SGLQMGLGFASFYDPMNSSYSPVIARWLGMIAILAFLATDGHLYMLSALAESFSTLPI
                     GNAMPNQGFYNVVNWGGSIFAFGLQIALPIVAALLITNIALGILTRAAPQLNLFAVGF
                     PITLTIGFVVLAISMPYLSPLLDRVVQEGLDKMLGILVKG"
     gene            complement(15583..>16465)
                     /gene="flgL"
                     /locus_tag="VFW59_07900"
     CDS             complement(15583..>16465)
                     /gene="flgL"
                     /locus_tag="VFW59_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEX5364179.1"
                     /translation="LANSTDGIGNYLFSGFQTRTPPFANTPGGVAYFGDDGQRLVQAS
                     ASRQLSSTNTGAELFMRIKDGNGTFTTGAAATNTGTGVISIGSLGNPPPTAAQAASTY
                     QVAFTVSPPATPGLPDVITYVVTDTTLATQVLPVPPLPAVPGVPYVSGQAISFNGIQF
                     DIQGVPKTGDTFTVAPSANESVFKTISDLVNLLNVEQAGGKLSNGIVRGLNNLDNALN
                     NVLTTRASVGVRLNELDALQNEGDNLGLQFKQSLSLLQDVDYNKAISDLTMQNTALQA
                     AQQTFSKVSGLSLFKYI"
BASE COUNT         3991 a         4933 c         4401 g         3140 t
ORIGIN      
        1 agttccatca aagcgatcgg cacacgccgc accgagatga gcgcgcgtct ggcaacggtc
       61 gagaagaact accgcgcaca gttctccaga ctggacgcga tgctgggcag catgaaccag
      121 accagcaatt acctcaccca gcaactggcc agcttgccga agtattaacc agggaaccaa
      181 catgaatgcc accacagcca tcagcgcgta ccaaagagtc ggcgtcgaat cgggcgtcgc
      241 tgcggccgat ccacacaagc tgatctcgat gctgtatcag ggagccttgc tggctatagc
      301 caatgcgaag agcagcatcg cacgcaaaga cattcccgcc aaaggcaagg ccatctccca
      361 tgccatcacc atcatcgacg aaggcctgaa tgccagcctg gacaagaaag tgggcggcga
      421 actggcgcag aatctgtcgt cactgtacga gtacatgggc tcgcgcctgt tgacggccaa
      481 tatcaagaat gacgtggccg cgctcgatga agtggcacgt ctgctgaccg acctgaagag
      541 cgcctgggac gacatacgcc cgactgtgac acccgccgct aatgcgacag ttgaaagcgc
      601 gcccaaacca atctacgcca gaggataacg ccccatgggt atcccacaag tcattgccaa
      661 ttacgaatcg ctttccgttc tcaccggaca gatgagcgaa gcggccagac agggcgaatg
      721 ggatcgcctg attgaccttg agcagcaatg cagccgtcag gtcagttcga tgaaaccaat
      781 cgatgctgcg gtcgctctgg acgagcctgc acggcagctg aagattcgcc tcatcaagga
      841 tatcctgtcc cacgacgccg agattcgcaa tcgcactcag acatggatgg ttcagttgca
      901 acgcatcctg caaagcaatc agcaggaaca aaaattgcac cgggcctacg gcggataact
      961 gcgatctgcc cgctcaaccc ggcggatagc tgccatgcaa ttggttatag caccgacttg
     1021 aactgaatca ccacccgccc tgacaggaca gtacctcacg ccaatgctgc ccgccaatgt
     1081 cgtcaatgca cttcaaacac tcgccaattc agcgaagccg ccgctcgcgc tcgacaataa
     1141 cgtccctgca gcgataagca aacttgaaac agggcagcaa ctgcaagcct ccgtcctgtc
     1201 caagttaggc gaagggctgt tcaaggtcca gctggcgggt caaaccatgc agatgcgctt
     1261 gccgggaaac atccagagcg gcgacacgat caagcttcaa gtcgtgtccg ttcagccacg
     1321 gatcaccttc agcatgatgg cgtctaccaa ccctctgtcc acgcccgacc agataggcga
     1381 tacggctcgc ctgctatcca accttaccga tcgtccgata gaaaggccgc tggttcaaca
     1441 gatgggaagc aaagccgtct gggcagcgac gctgcaggct cccgacgtcc ggcaacttgc
     1501 gacgggtctg cacgatgcgc ttagcaagag cgggctgttc tatgaatcgc atcaggcgca
     1561 atgggttcga ggcgaacgca gcatcaacca gttactggag gaacctcaaa acctcctgac
     1621 cgggaaatcg ttaacgctct cacccggcga cacgcagcaa agcgctcagg ataagctcgc
     1681 ggcggcacag ccatcccagc catcggccaa gagcgataac gccccgctca tcgccaaaga
     1741 attgcagccg ctggtacaac agcaactgca cacgctggaa aaccatcaca tggtatggat
     1801 ggggcaagtt tggcctgaac aacagatgca atgggaaatc aagggagaac cgaaacatca
     1861 aggccggcag caggatgagc ggcaatggag caccgaaatg gagctatccc tccctaaact
     1921 gggcgatgtt cgcgcccggc tggtatttgc cgaaagtgga ctgaggctaa cgctgcatgc
     1981 agcggatgca gcaaccacag cactcttcaa ccgatccttg ccgcagctta aaaactctct
     2041 cgacaatgcc ggcatcgcac tcaccgccgc gatagtggag aaaccatgag cgacgccaag
     2101 ccctccgcac aacaggtggc catcgccctc gcctatggcc agggtaacag cggcgcacct
     2161 aaagttgtcg ccaaagggcg cggcctggtt gctcaggcaa tcatcgagcg cgccaaacaa
     2221 aacggagtat tcgtgcacga atccgaagat ctggtcggat tgctgatgaa ggtcgaactc
     2281 gatcaggaaa ttccgccaca actgtatatg gcagtagccg aactgctagc ctggctgtat
     2341 cgccttgagc atgcagatgc tcccgtgcct ccgccattca tgaagctcta actttttatt
     2401 ccatggccag tttgttatca tcgcggcgat gaaaaaagac atccccctga ccatcgagac
     2461 cattaccgag gaagacgaac aggaatgcct cgtcacttca gccagacaaa taaaatccat
     2521 cctgcgcacc atagctgatg ccgggaccaa tagcgcgctt tactacaccc ccaatcataa
     2581 cttcatcatg acctccgtac tggatgtcga tgaggacggc ctgtggatcg aacaaggcag
     2641 cactgccacg gtcaattacc tcatcaccga gagcaagaag ttaacgctgg tcagttcgca
     2701 tgcacaggtc aaggtgcaat tcaccgtaga tgtcgctgcc agtgtaagtt atgacggtta
     2761 tcccgcattg tttctacccc tgccaagcaa gctctatcgc ctgcagcgcc gcgagttttt
     2821 cagattatcc ttgccaccca acgagcagct gaactgcatc atcaatgtcg ccaaggcgga
     2881 gaggcgctca gaagcatcgc cgcccgtcga tttggcggtg agtgacctcg aaatacctgc
     2941 tgcggacatc agcggtggcg gcatcgggct tatttgcaga caagacgatg tcgagctcca
     3001 gcccggagaa acctattctg gctgccagat cacacttcct ggagccggcc ccatcttcgt
     3061 caatttcacc gtcatgaatc tggtcatact gagcacgacc agatcaggca agacgattaa
     3121 acgcgcgggc tgcgaattca cgaacctcga cagccaaaca acggcgaaac tgcagcgctt
     3181 catcaccgac atgcaacgga acaaggccgc caacagtttg ctggtataac gcgtcattcc
     3241 cgcccacgca tgaattgaac gggcttctag gccaaaaatc tgccagccca tgaaaatcac
     3301 ccccgaattg gatttttccg agcacaatca ggtgaaagcg gccccgcttt cgcccggcct
     3361 gcgcgaacac gccttagcgc gcattcctca ggcatttgtg ttgctctagc tttgtttcct
     3421 atgcacagtt tgctattatc cgcgcgatga aaaaagatat tcccttgacc atagagagca
     3481 ttaccgacgc tgacgagcaa gaatgccgca tcacgtcatc caggcaaatt gcgtcgatat
     3541 tgcgcaatat cgccgagacc gggtcaaacg ccgcgctgta ctacaactcc aagcgagact
     3601 tcatcatgac caccattctg gatgtcgatg aagacggcat gtgggtggaa caggcaagca
     3661 gcgcaacggt gaaccaccag attgaagaga gcaagagaat caagctagtc agttcgcaca
     3721 accaggtgaa agtccagttt ggggcggatg gagcgagcag cgttacctat gatgagcgtc
     3781 ccgccttctt tataccgata cccgacagca tctatcgctt tcagcgccgc gaatacttcc
     3841 gcctgtcgct actaccttcc gaacagatgc gctgcatcat caatatggcc cgcccagagg
     3901 cgccaggcac catagtccag attgaggtgc cggttgccga catcagcggc ggcggcatcg
     3961 gattgacctg catggaaggt gaggttgata ttaacgcagg ggaaagctac tcaaaatgcc
     4021 agatcaatct gccgggagtg ggtcctgtgc tggttaacct gatcgtcagg aacctcgttc
     4081 ctgtttccac caccaagtcc ggcaagacca tcagtcgcgc cggctgtgaa tttaaagatc
     4141 tggatggtca gaccaccgta aaactacagc ggttcatcac tgacaaacag cgactgatgg
     4201 ccgccaacgc gacctcgctg taacctccca gagcgatcaa acctgcatgt tcatgatgtc
     4261 gttgtaggcc tgcacaaccc ggttacgcac ctgaattgcc gtctggaaat tgatactcga
     4321 tttctgcatg gcgatcatgg tgtcgctcag gctgacgcgc tcgtccccca gcacaaaggc
     4381 cttgcgcatg tcttcggatt tgctctgagc ctgagccacc ccgtccagcg cagattttaa
     4441 cgcatcagag aagtcgacct tccctggctg cgtcgcaacc tgactgggct cacgcattcc
     4501 cgtcgcggcg gccttagcca cacgcatttg cgccaacaaa ctatccactg ccgatgtatt
     4561 cactattccc cctttttcag gcagtgccac attaaccgaa ggggcgccat acagacgcgg
     4621 cgaaaaggca tggatttaca gcggtattca ggttttcaaa tacctatgag tgcatcgata
     4681 atgtgcacag cgtccgaacc ttcaacctca ggaacgaccc gtaatcacta tggcagaaca
     4741 agcagacaac gttgcgacca atcgcttcaa ccggctcacc cttcagcaaa aactggggct
     4801 gggagcgggg gttgctgcgc tattcgcact gattgccggc gcgtggatat gggggcaaac
     4861 gccggaatat cgggttttat acagcaatct atccgatcgc gacggcggcg cgatcatcgc
     4921 ctcgctgcag cagatgaaca tcccctacaa gtttgccgag gggggcggcg cgctattggt
     4981 gacctccaat caagtacatg aagcacggct gaaactcgcc tcgcaaggac ttcccaaggg
     5041 cggcaatgtc ggttttgaat tgatggaaaa tcagcgcttc ggcatcaccc agttcgccga
     5101 gcaagtcaac taccagcgcg cgcttgaagg cgagctggcc agatcggtag aaagcatagg
     5161 cgccgtcgcc gcagcaaggg tgcaccttgc catccccaag cccagcgttt tcgtcaagga
     5221 acaccagcaa cccagcgcct cggtcgtctt gagcctgcaa ggagggcgcc tgcttgattc
     5281 ggcccaggtc agcgcgatca tccatctgat ttccagcagc gtgccggaga tgtcagccaa
     5341 gaatgtgacc gtagtcgacc agaacggcac gctactgagt gccgatcaca acggcaacgt
     5401 gaacgaaggg ctcgacgcga accagctcaa gtacgtgcaa caaatcgagc acagctacat
     5461 acagcgcatc gaaatgttgc tcggccccat gctgggcgca aacaacctgc gcgcccaagt
     5521 gtctgccgac atcgactttt cacgcaccga gcaaacctcc gaggtcttca aaccgaacca
     5581 acctcccaac gaaagcgcgg ttcgcagcca gcaaatctcc gagaccctga atggcaacgg
     5641 tctcacggcc agcggcgtgc cgggcgcgct aagcaatcaa ccgcccgctc cggccaccgc
     5701 ccccatcgtc gccgcctcgc ccgccgtggg cgtcaacgca ggcacagacc tggcaggcac
     5761 agacctgaat gtatcgaacc tgcaaaaaga gtccaccatc aattacgagg tcgaccgcac
     5821 catacgccac accattcttc ctgtcggcgc gatcaagcgc ttgtctgttg cgctggtggt
     5881 caatggcaac cgcaaggtgg tcgatgcaaa aggaaaaagc gtcacgaaac cctttaccga
     5941 cgcagaaaaa gaacagatca acaatctggt caaagaggcg atggggttcg accagaatcg
     6001 cggcgacagc ctgaacgttc agatcgcctc tttcgccgac acgactgaaa atattgaaga
     6061 aacgccatta tggaaacagc cggacgcgat tgagctaggg aaggagctgc tcaaatatgc
     6121 attgatcgcc gcgatcatgc tatttgtcgt tttccgtatc atcaaaccgg cgttccaaag
     6181 cctgattaac ccagcgagcg aaaccggtgg attctctgcc gcccccgaga tcgtcgagcc
     6241 ggaagaggcg ggcgcaacgt acaaaccgac ctcgcctggc ttcgagagca ccttgatggc
     6301 cgccaagcaa cttgccatgc aagaccctaa aatcgtcgcc agcgtaatca aggaatgggt
     6361 ggagcaatga tcaacggcat agatagaagc gcgatttttc tgatgtcgct gggcgagaca
     6421 gaggctgcag cggtcctcaa gtacctcgaa cccaaggagg tgcagaagat cagcgcggcc
     6481 atgatgaagc tgaagaacct cagccgcgaa gacatcgcgg gggtattcaa tgacttcatg
     6541 atttcagcag cgggcaagac cgacatcggc ctggactcca atgagtacat ccgcaacatg
     6601 ctcatcaagg cgctgggcga cgacaaggcc gccggcctgc tggatcgcat catgcacaac
     6661 agcgatacca gcggtatcga gagcctgaaa tggatggatc cgggagccgt agccgaaatg
     6721 atcggcaacg aacacccgca gatcattgcg accattctgg tgcatctgga accagatctc
     6781 gccgccgaaa ttatgaagat gttcgccgag cgaacccgca acgatgtgct gctgcgcatc
     6841 tccacgctgg atggcgttca gccgctggcc ttgcgcgaac tcaacgatgt cttgagcaag
     6901 ctcctgtcag gcggcggcac cggcaagaaa acgctgcacg gcggcgtcga gaccgcagcg
     6961 gaaatcctca acttcatggg ccacacgctg gaagccgaaa tgatggagaa tgtgcgcagc
     7021 tatgatcctg aactggcaca gaagatcgaa gacaagatgt tcgtcttcga gaatgtgctg
     7081 gagatcgatg atcgcggcat ccagctgatc ctgcgcgaag ttcagtccga gtcgctgatc
     7141 gtctcgctca aaggcgcgag ccaggaattg cgcgagaaga tattcaagaa catgtcttcc
     7201 cgcgcatccg aaatgatgcg cgaagatctc gaggccaagg ggcctgttaa actgagcgag
     7261 gtcgaggcga atcagaagga aatactcaag atcgtgcgtc gcctggccga cgaaggccag
     7321 atatctctag gtggtaagag cgaggaggct tatgtctagc gtactcattc ccaaggaaga
     7381 gctcacagca ttccagcgct gggagttggc ttccttcgat ccgcctccgc cccccaaaga
     7441 gcacgtgctc acccccgaag aagagcaggc agagatcgag cgcgtcaccg ccgccgaact
     7501 ggagcgcatt cgccagcagg cctatgacga gggctatgct caaggacagg aagcaggcta
     7561 cgccagcggc tccgcacaga ttcaggccga gattggacag ctccagacag cgatgcagga
     7621 gctgcacacg gcactcaacc aggtcgatac ggaactggct caatcgctgc tggacttgtc
     7681 gcttgagatt gcgcgcaaaa tggtgggcga ggcactcaag ataaagccag aattcatact
     7741 gaacaccgtt agcagcgcta tcggcaattt gccgcatttc aatcagcacg cccatctggt
     7801 cttgcacccg gaagatgtcg aactggtgcg caagcacatg ggagacaacc tggcgcatgg
     7861 cggctggaaa ctgatcggcg acagcagcat caagcgcggc ggatgccgcg tcgagaccac
     7921 tcacaccaag gtcgatgcca ccgtcgaagc gcgctggaaa cgcatcgtcg aatcgatcgg
     7981 agaggacgaa tcatggctag cctgaatcca tcctctcata gcgccaaatg gcgcggcctg
     8041 ctcgacgaaa gtcgcgaaca catcgcgcaa tgccagacca tgccagctag cggccacctg
     8101 acaaaagtca ccggcttggt gatggaggcg gtcggcctga agatgtcggt aggcagcaca
     8161 tgcctgatcc acttgccgag cggcgtggta gcggcggaag tcgtcggctt ctccggcgaa
     8221 aaactgttcc tgatgcctga aaatgatgtg tatggcctgg tgcccggcgc acgcgtgaca
     8281 ccgatcgact ccatccatat cccaaccttg ggcagcaaac caccgctccc cgaactgcgc
     8341 gccagcgatt cagccaaaca actacccgtc ggcgacatgc tgctcggtcg agtcgtggat
     8401 ggtgcgggcc gcccgctcga tcaattcggc ccgttaaaca acaccagctt tgcgccgcta
     8461 caaagccgcc ccttcaaccc gcttgaacgc gcacgcatcg acaccgtact cgacgtcggc
     8521 gtgcgcgcca tcaacagcct gctgaccgtc ggtcgcgggc aacggatggg actgttcgcc
     8581 ggcagcggcg tgggtaagag cgtcttgctc ggcatgatgg cgcgctacac caatgccgac
     8641 atcaccgtgg tcggattgat cggcgaacgg gggcgcgagg tcaaggagtt catcaacgac
     8701 attcttggcg aggaaggatt ggctcgttca gtgatcgtcg ccacccccgc agacacatcc
     8761 cctttgatgc gactgcaagg cgcggcctat gcgacaacca tcgccgaata tttccgcgat
     8821 caaggcaaga acgtcttgct gatcatggat tcgctgaccc gctatgccat ggcccagcgc
     8881 gaaatcgcac tggcggtcgg cgaacccccc gccaccaagg gttatccacc ctcggtgttt
     8941 gccaagttgc cgcaactggt agagcgcgct ggcaacggcc tggagggcag cgggtcgatc
     9001 accgcctttt atacggtgct aaccgaaggt gacgaccaac aagaccccat cgccgacagt
     9061 gcgcgcgcca ttctcgatgg ccacatcgtg ctgtcgcgcc gactggtaga acagggacac
     9121 taccccgcca tcgacatcga ggcgtcgatc agccgagtca tgtcccatct ggtctctccg
     9181 caacatcaag cgctggtaca gcgcttcaaa cacctgtacg ctcactacca acgcaaccgt
     9241 gacctgatca gcgtcggcgc ctacgtgcgc ggcagcgacc cactgctgga cgaggccatc
     9301 ctgctctacc ccaggatggc acagtttctg aaacagggca tgcacgactg cgagacctat
     9361 caggcgagcg ccgcacaact ctcggcgctt tttgagccag tacactagtg gactgaatca
     9421 tggcgacccc tttctctctg acaacgctgt taaatctggc tgaacaccac agcgattctg
     9481 cgacgcgcaa gctcggccag ttaaatcagc aggaatatag cgcgcaacag caattggaga
     9541 ttctgcttga gtttcgccgc gattatcaag ctcagatgca gaaagacacg gaggcgggta
     9601 tgagtcccgc tgcattgcgc aattttcaac aattcatcta caagcttgat gaggccattg
     9661 cgcagcaacg caggcaactc gaaaacagca aggcctcgac ccagctcggc cgcgatgaat
     9721 tcgacgtgac tcaacgcaaa ctgaaatcgt tggacacgct acgacagcgc catattgaaa
     9781 cgcaaaaaca aatcgccaac aaggcggaac agaaggaact ggacgaacac accggccgca
     9841 tggccgcccg ccgcatgagc gacgaattga atcaggctgc ggaaaacgag aaatagaccg
     9901 cgcgatccca cataaaggaa ataactatga cgcccgtaca gatcagcccc gccccacagc
     9961 cctctggcaa cgccaaggca tcgtcgcaaa cagacagcgc gacaaacaat cagaccgccg
    10021 atgggcagcc tgccgaaccc ttcgccaagg tactgtcacg ccaggttgac aagagtgact
    10081 cgcccgctca gaagacggac gcagcagacg ctggcaagac tgatgcgaca cctcagaccg
    10141 ccgcccctgc cgtcgtccag cccgatgcgc tggctgcaat catgtcgcaa atccccgtgg
    10201 aaatgcgcgg agcaaatgtc acagaccgcg ccgccctcat cagcacggag ggccgcctgc
    10261 ctgcgggtat tgatacggca ctgcccaaaa tagatgcggc cctctctcag attgatgcac
    10321 gaacacaggg gaaagccacc acctcacctc aagccgatct gtccgctgcc gatgcgcaca
    10381 aaaaaacacc cgcaggatcg cgaaacggtc aggataaaga ttttgcctcg acactcaagg
    10441 ccgagaccaa gtcgcttgca gccgataccg atgcaatgca taaaagcatg cttggtcgca
    10501 ccgacattac cagcgatgca tctcagctag tcgcgtcaca aaccagcgcc gcctccacga
    10561 tctcgcaggc aacgctcgcc gcgatgaccg gcacctctat cggcaaccaa cccgccggac
    10621 cagcccagac catcgcgaca ccgcttggca gcgcagcctg gggggatgat ttctcgcaaa
    10681 aggtcagctg gatgattacg caaaagaatc aggtcgccga actgcacctg aaccccccga
    10741 atctcggccc gctggatgtg gtcatcaaga tcaccgacaa tcaggctacc gccctgttca
    10801 cctcgcctca cgccatggta cgcgatgcgg ttgaaagcgc tttgcctaaa cttcgcgaac
    10861 tactggccga taatgggatc atgttgggca atgcgacggt tggcgatcaa tcaccgcgcg
    10921 aacgtgaagc cggggaattt tcaggcagga ctacaggcac atcggcgcag cgggatactg
    10981 ataccggcct gacagaaccc actgttccgg ttgcggcggc acgacgacac aacggtgtgc
    11041 tggacacatt tgcttgagcg ccccataacg ggctacatta aaagaagatt acgagttcaa
    11101 acgcgttatc attcgcgcct gaatttgagc aaactaccgt aaggacagaa tatggcaaag
    11161 ggaaccaagc caggtgcaaa acctgaagag caggaagaag tcgctccacc ccaaaagagc
    11221 aaaaagaaac tgatcatcat tatcgtcgcg gcggtgctcg tcataggtgc ggctgcagtg
    11281 gcggctgtac tgttgctgaa gccagctcat cctcgccaga gcgaaaccga agaaacgacc
    11341 agcgagcata aggttgaaga ttccaagttc ccagccaaat ttatcgacgt aggcaccttc
    11401 accaccaacc tcgcaccaga cgagggtgac cgttttgtgc aaactgccat tacattcaaa
    11461 atcagcaaac ccgaactgga agaaaaaatc tctgccaccc ggcccgaatt gcaacaccgc
    11521 attaacatgc tattgcaaag caaacttcct tcagagctgg cggcttccga gggaaaaacg
    11581 caactggctg ctcagatcaa attgcaggcc gaagaggtat tgggattgcg taaagccacg
    11641 ccaacagcag gtgccgaacc agcagctggc ggccccgtcg cggccaagcc ccaggcaggc
    11701 ctggacgaag tgctgtttac ctcatttctc attcagtaaa acagaatcga acgataacga
    11761 acgatgagcg aatttctctc acaagatgag gtcgactccc tactcaaggg ggtcaccggc
    11821 gatacggacg aggtcaacga ggacgaagca tcggcccccg gaactgttcg ctcgtacaat
    11881 cttgcttcgc aggaacgcat cgtacgtggg cgcatgccca cgatggaggt tatcaacgaa
    11941 cgtttcgccc ggctgttccg tatcggcttg ttcaatttca ttcgccgcac acctgaaatt
    12001 tcggtcggtc cggttcgagt tctaaaattt ggcgagttca tccgcaacct gcatgttccc
    12061 accaacctga acatcattca ggccaagcca ctgcgcggca atggcctgtt tattttcgac
    12121 ccgaatctgg tgtttcttgt cgttgacaac atgttcggcg gagatgggcg attccatacg
    12181 cgcgtagagg gacgcgagtt cacccagaca gagcaacgca tcatccagaa tctgctgctg
    12241 gtggtgttcg agacctatgg caaagcctgg gaagccgtac atccactgga gttcgagttc
    12301 gtgcgctccg agatgaatcc gcagttcgcc aatatcgcca caccgaacga ggtcgtcatc
    12361 gtcacgacat tcgatattga gctgggcggc aatggcggat cattccacgt ttgcatgcct
    12421 tattccatgc tggaaccggt caaggatctg ctctacagca ccatgcaggg cgaacatctg
    12481 gcaatggatc atcgctggct gcaacttctg tccaaacagg tacaatccgc acaaatcgaa
    12541 ctgattgcgt cacttggaag tgcacgcatc accatggagc aggtactgcg catgcgtagc
    12601 ggcgacatca tcccgctctc agtcaacgat aacatcaccg ctaccgtaga cggtattccc
    12661 gtgatggaat gtaaatatgg atcatttaat aatcagtacg cgctgagagt cgaaaaaatg
    12721 ctttcgacca gcgaaggaga aggtaatgcc tgacgaatcc ggcctcaacg aagacgcgat
    12781 aaccgccgac gattggggcg ctgccatggc agagcaaaca accactgcca ctgccgaaga
    12841 cgactggggt gccgcattgg ccgaacagac cgccgcagag aaggcgcctg ccgaaccggc
    12901 caaaccgcat gtgtttgaaa agttcggctc tggtggcagc ccgaccagcc agaacgatct
    12961 ggacatgatt ctggatatcc cggtcaattt gaccgtcgag ctgggacgca ccaagatgcc
    13021 gatcaaaaat ctgctgcaac tcgcacaagg ctcggtcgtc gaactggcag gcatggcagg
    13081 agaaccgctc gacgtactga tcaatggctt cttgattgca caaggagagg tcgtggtggt
    13141 gaacgacaaa ctgggtatcc gtttaaccga catcatcacc cccgccgaac gcctgcgccg
    13201 cctgaacaga tagcatgcca gttcaatttc gttacgcctt cgccgcattc tcattggcgt
    13261 tcgcccagct gactgcggcc gttgaaggaa cgcggccggg ttacgtcccg ccgcctcccg
    13321 ccgtcagttc cggcagcatc gtacagatca tcttcagcct gctgctggta cttgcggtcg
    13381 tcgttctggt tggctggctg ttgaggcgca tgaacctgtc ccaacaaggg acgggcagcc
    13441 aactcaaaat gataggcggc atcgcaatag gccagcgtga gcgcatcgtt ctcatcgaaa
    13501 tcgatgacac ctggctggtg gtaggggttg ggcccggtca gatacgcacc ctgcacacac
    13561 tccccaaacc cgagggagat acggcaacgt ccagagccgg tcaacctgac cagaccgaag
    13621 gcaagtttgc cgcgctgctg tcgtccatgc tcaaccaacg cccatccgcc aagaagtccg
    13681 atgcgcccta gctctcttgc aatactggcg gtgatcgcac tcaccgcact ggcgcagcca
    13741 gcttgggccg aaatgggcat tccagccctg accagcagcc ctgcacccgg cggtggccaa
    13801 acctatagct tgagcctgca gacgctgctg gtgcttacct cgctcagttt tctgccggcg
    13861 atcatgctga tgatgaccgg tttcacgcgc atcatcattg tcctgagcct gttacgttcg
    13921 gcgcttggta cgccatcctc gccccccaat caggtcttga tcgggctgtc gctgtttctc
    13981 actttcttcg tcatgtcgcc ggtattcgac aagatgtaca tcgatgccta cctgccctac
    14041 agcgaaaaca agatggactt cactcaggcg gtagacaagg ccagcatccc gctgaagact
    14101 ttcatgctgc gccagacgcg cgaggccgat cttgcgctgt tcgtccgcat ttcaaacagc
    14161 gaaccactgc aaagtgccga tcaggtaccg ctgcggattt tggtaccgtc ttatgtcatc
    14221 agcgagctca agaccgcatt ccagatcggt ttcgtcatat tcattccatt cctcatcatc
    14281 gacatggtgg tcgccagcgt actgatgtcc atgggtatga tgatgctgtc gccctccatc
    14341 atttcgctgc ccttcaagat catgttgttc gtattggccg acggctggaa tctgatcatc
    14401 ggttcactgg cccaaagttt ttatacctga tgagacggcc acatcatgac tcctgaaacc
    14461 gtcatcacca tcggggaaca agcggttgaa atgaccctga tggtctcggc ccccttgctg
    14521 ttgaccgcac tggtcatcgg cctggtggtg agcatctttc aggccgcgac ccagatcaac
    14581 gaaatgacgc tatccttcat tcccaagatg ctcggaatct ttgtggtgct gatttttacc
    14641 ggcccctgga tgatcagcat gctggtcgat tacattcagc gcctgtacgg caacataccc
    14701 tggatgatcg ggtaatcaga acgtgctgcc agtcacatga tcagcatcac cagcgcgcag
    14761 ctcgatgcct ggctcgccac gttgttattc ccgctggtgc gcatcttggc catgatctct
    14821 accgcccccg tgctcggcaa caagcaggtt cccaagcggg tcaagatcgg cctgtcggcg
    14881 ctgctggcca tcctcatcgc cccgacgctt tcatcgattc cgctggttcc agtaggctcc
    14941 ccgcagggat tgctgatcat catccagcaa atcatcatcg gtgtggcgat gggatttacc
    15001 atgcgcatgg tcttcaccgc cgtcgaaatg gccggcgaat tgtctggact acagatgggg
    15061 ctgggatttg ccagcttcta cgacccgatg aattcgtctt attcacccgt catcgcgcgc
    15121 tggctcggca tgatcgccat cctggctttt ctggcgacgg atgggcatct gtacatgctg
    15181 tcggcgctgg cagagagctt cagcacctta cccataggca atgccatgcc gaaccagggc
    15241 ttttacaatg tggtcaactg gggcggcagc atctttgctt tcggcttgca gatcgcgtta
    15301 cccatcgtgg ccgcgttgct gatcaccaat atcgcgctgg gcatcctcac ccgcgcggca
    15361 ccgcaactca acctgtttgc agtgggcttc cccatcacgc taaccatcgg tttcgtcgta
    15421 ctggcgatct cgatgcccta cctcagccct ctgctggaca gagttgttca ggaagggctg
    15481 gacaagatgc tcggcatact ggttaagggc tagccgagct gataaaaaaa tcgttcgttc
    15541 aggccactga acgaacgatg attggcatct tgaaaccgcc gattagatgt acttgaacag
    15601 cgacagccct gaaaccttgg agaaggtctg ctgcgccgcc tgcaatgcgg tgttctgcat
    15661 ggtcaggtcg ctgatggcct tgttgtaatc gacatcctgc aacaaagaca gggactgctt
    15721 gaattgcaga cccaaattgt cgccctcgtt ctgcaaggca tccagctcat tgagccgcac
    15781 ccccaccgat gcacgggtgg tcagtacgtt attcaacgca ttgtccagat tattcaagcc
    15841 acggacgatg ccattgctca acttgccgcc agcctgctcg acattcagca agttaaccag
    15901 atccgagatg gtcttgaaca cgctctcgtt ggcactgggt gcgaccgtga aggtgtcgcc
    15961 agttttgggc acgccctgaa tatcgaactg gatgccattg aaactgatgg cttgcccact
    16021 gacgtatggc acccccggta cggcgggtaa aggtgggacc ggaagcacct gagtagctaa
    16081 cgtcgtatcc gtcaccacat aggtgatcac atccggcaaa cccggggtag ccggaggcga
    16141 cacagtaaac gcgacctgat aggtgctggc tgcctgagct gctgtcgggg gcgggttgcc
    16201 caaggagccg atgctgatca cccccgtacc ggtattggtc gccgcagcac cagtggtgaa
    16261 cgtaccgtta ccatccttga tgcgcatgaa caattctgcg cccgtattgg tggacgacaa
    16321 ctgacgcgat gcgctcgcct gcaccagacg ctgaccatca tccccaaaat acgctacgcc
    16381 accgggagta ttggcgaagg gaggggtgcg ggtctggaag ccggagaaca gataattgcc
    16441 gataccgtcg gtgctgtttg ccaag
//
LOCUS       DASUIV010000217         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_425026, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000217 DASUIV010000000
VERSION     DASUIV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425026"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..1526)
                     /locus_tag="VFW59_07905"
     CDS             complement(348..1526)
                     /locus_tag="VFW59_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5364180.1"
                     /translation="MTDSGNRPDQCDIAIIGGGPVGAALAVALKDSGLDIRVLEARQA
                     DTASVDARALALSCGSRLLLDRLGVWNKLDPVSSIRTIHVSQRHSFGRAVLRAAELNV
                     PELGYVLPYPVLQDALDSAMRGSAAQVVYGAAVTQLKGEADLAVIGYRHEDSEHELHT
                     RLAVVADGGRLLAQQYPPEVRDYGQSALITHVTCTSPKPDTAYERFTTQGPLALLPYQ
                     NGYELVWTAPHERAQEMLGWNDAKFLIELHQHFGDRVGSFTGVGKRSCFALGLKRAPQ
                     QTPMPHTVLLGNAAQTLHPVAGQGFNMGVRDAWELAQEILDAAPNELGTETMLSAYRN
                     SRRTDREIGIRFTDGLVRLFSNDLPMLTPARAAALSALDILPFAKKFVAKRMMFGANG
                     "
     gene            complement(1519..2826)
                     /gene="pepP"
                     /locus_tag="VFW59_07910"
     CDS             complement(1519..2826)
                     /gene="pepP"
                     /locus_tag="VFW59_07910"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEX5364181.1"
                     /translation="MSLDPTPYVQRRARLLEKMQHGIAVIPTAPEVPRNGDTHYEYRH
                     DSNFYYLTGFAEPEAVLVLISGDEPQSILFCRDKNLEREIWDGFRHGPEAAQESFGFD
                     AAYPIDQLDDKLAELMGNQPVLFYPLGKDAAWDQRLLKLRAAVQAKVRSGVRAPDEVR
                     DVRALLHEMRLFKDGHELDIMRRAAAISTQAHRRAMRFVRPDQFEYEVEAELLHEFCR
                     QGARHPAYTSIVAAGSNACVLHYVDNDARLEEGELLLIDAGCEVGGYASDITRTFPVN
                     GRFSSAQRDVYEIVLASQAAAIAAAQPGAHWEAPHQAALRVLAQGFIDLRLCRGSVDE
                     VIEHESYKRFYMHRTGHWLGMDVHDVGDYKIGADWRPLQAGMVLTVEPGCYIRPADDV
                     PLDLWNIGIRIEDDVLITATGNEVLTDAAPKTVAAIEETMRHD"
     gene            complement(2823..3569)
                     /locus_tag="VFW59_07915"
     CDS             complement(2823..3569)
                     /locus_tag="VFW59_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5364182.1"
                     /translation="MILAAGRGERMRPLTDHTPKPLLPVGGKPIIVWHIEQLVRAGIT
                     ELVINHAHLGHQIEAALGDGSQFGASIRYSPEASALETAGGIANALPLLLPSPACGES
                     VARGAQPAGGEGEPFAVINGDIFCDYDFSLLPARASALLASGDSAHLVLVDNPEQHPD
                     GDFHLRNGRILPSDDSSRLTFSGIGLYCPELFATSPRGSVAPLAPLLRQQIAAGRASG
                     EHHRGWWVDVGTPQRLTELDSRLRSQQNAT"
BASE COUNT          582 a         1114 c         1259 g          692 t
ORIGIN      
        1 catataaaca aggcgggatg ccaacgcaac atttcaatgg taggcgtgct cgcctaccat
       61 tgaccgcttt gctgtgattt cccctgtgcg ccgccgagta gcgcaggctg gtcaggggat
      121 gtcggcgagg actgtctgag cgcgtagcgc gagttccgca gccgcctgac cagacgagca
      181 acgcagggaa ccccgtaggg gcgggaaacc ggggtcgcct tttcttcggt taccttcttt
      241 tggcgaagca aaagaaggta acttgctgtc gggcaacccc cgacggtttt gatctggatt
      301 ttgacatcgg cccttcgacg ggctcagggc gaacggccaa tattcgacta cccgttcgcc
      361 ccgaacatca tccgcttcgc gacaaacttc ttcgcgaacg gcaggatgtc cagtgcgctg
      421 agcgcggcgg cgcgggccgg ggtcagcatt ggcaggtcgt tggagaacag ccgcaccagc
      481 ccgtcggtga agcggatgcc gatctcgcgg tcggtgcggc ggctgttgcg gtaggcggac
      541 agcatcgtct cagtgcctaa ctcattgggt gcggcgtcga gtatctcctg tgccagttcc
      601 catgcgtcgc gcacgcccat gttgaaaccc tgtccggcca ccgggtgcag ggtctgtgcg
      661 gcgttgccca gcaggacggt gtgcggcatc ggggtctgtt gcggcgcgcg tttaagtcct
      721 aatgcgaagc agctgcgttt gccgacgccg gtgaagctgc cgacgcggtc gccgaaatgc
      781 tggtgcagct cgatcagaaa cttcgcgtcg ttccagccca gcatctcctg cgcgcgttcg
      841 tgcggtgcgg tccacaccag ttcgtatccg ttctggtagg gcagcagcgc cagcgggccc
      901 tgagtggtga agcgctcgta tgcagtgtcg ggcttagggc tcgtgcaggt gacatgggtg
      961 atcagcgcgc tctggccgta gtcgcgaact tcgggcggat attgctgcgc cagcaatcgc
     1021 ccgccgtcag cgaccaccgc gaggcgggtg tgcagttcgt gttcgctgtc ttcgtgccga
     1081 tagccgatca cggcgaggtc ggcttcgcct ttcagctggg tgacagcggc gccgtagacg
     1141 acctgtgctg cggagccgcg catcgcgcta tccaaggcat cttgcagcac cgggtaaggc
     1201 agcacgtagc caagttcggg gacattcagt tctgcggcgc gcagcacggc gcggccgaag
     1261 ctgtgacgct gtgagacgtg gatggtgcgg atggatgaga ccgggtcgag tttattccac
     1321 acgccgaggc gatccagcag caggcggctg ccgcaggaca gcgccagtgc gcgggcgtca
     1381 acgctggcgg tgtccgcctg gcgggcttcc agcacgcgga tgtccaggcc gctgtctttc
     1441 agtgcgacag ccagtgctgc gccgaccggg ccgccgccga tgatggcgat atcgcattga
     1501 tcgggtctgt tgccggaatc agtcatggcg catggtctcc tcaatcgcgg cgacggtctt
     1561 cggtgccgca tccgtcaaca cttcattgcc ggtcgcggtg atcagcacgt cgtcttcgat
     1621 gcggatgccg atgttccaga gatccagcgg cacgtcgtcg gcggggcgga tgtagcagcc
     1681 gggttcgaca gtcagcacca tgccggcttg cagcgggcgc cagtcggcgc ctatcttgta
     1741 gtcgccgaca tcatgcacgt ccatgcccag ccagtggccg gtgcggtgca tgtagaagcg
     1801 cttgtagctt tcgtgttcga tcacctcgtc cacgctgccg cgacacagcc tgaggtcgat
     1861 gaatccctgc gcgagcacgc gcaacgcggc ctgatgcggt gcttcccagt gtgcgccggg
     1921 ttgtgcggcc gcgatcgccg ccgcctgtga ggccagcacg atctcgtata cgtccctttg
     1981 cgcggacgag aagcgcccgt tcaccgggaa ggtgcgcgtg atgtccgagg cgtagccgcc
     2041 gacttcgcag ccggcatcga tcagcagcag ctcgccttct tctagccggg cgtcgttgtc
     2101 cacgtaatgc agcacgcagg cgttcgaccc ggcggcgacg atggaggtgt aggcggggtg
     2161 gcgcgcgccc tggcggcaga attcatgcag cagttcggcc tcgacctcgt attcgaactg
     2221 gtcggggcgg acgaagcgca tcgcacgccg gtgcgcctga gtcgagatgg cggcggcgcg
     2281 gcgcatgatg tccagttcgt gcccgtcctt gaacagccgc atctcgtgca gcagagcgcg
     2341 cacatcgcgc acctcgtccg gtgcgcgcac accgctgcgc accttcgcct gcacggcggc
     2401 gcgcagcttg agcaggcgct ggtcccaggc tgcatccttg cccagcgggt agaacagcac
     2461 tggctggttg cccatcagct cggcgagctt gtcgtcgagc tgatcgatcg ggtaggcggc
     2521 atcgaagccg aagctttcct gtgcggcctc ggggccgtgg cggaagccat cccagatctc
     2581 gcgttccaga ttcttgtcgc ggcagaacag gatcgactgc ggttcatcgc cggaaatcag
     2641 caccagcacc gcctcgggtt cggcgaagcc ggtcaggtag tagaagttgc tgtcgtggcg
     2701 atactcgtaa tgggtgtcgc cgttgcgcgg cacctcgggt gcggtgggaa tgacggcgat
     2761 gccgtgttgc atcttctcca gcagccgggc gcggcgctga acgtaggggg tcggatcaag
     2821 gctcatgttg cattctgctg tgaacgcagg cggctgtcga gttcggtcag gcgctgcggt
     2881 gtgccgacat cgacccacca gccgcgatga tgttcgccgc tggcgcggcc tgcagcgatc
     2941 tgttgccgca acagcggcgc gagcggggcg acgctgccgc gcggactggt ggcgaacagt
     3001 tcgggacaat agagaccgat gccgctgaag gtgagtctgg atgagtcgtc cgatggcaga
     3061 atgcgcccat tgcgcagatg gaagtcgcca tcggggtgct gctctggatt gtcgaccagc
     3121 acgaggtgag cgctgtcgcc gctggccagc agcgcagacg cgcgggcggg cagcagcgag
     3181 aagtcgtagt cgcagaagat gtcgccgttg atgacggcga aaggttctcc ctctccccca
     3241 gccggctgcg cccccctcgc tacgctctcc ccgcatgcgg gagaggggag taaaagtggc
     3301 agtgcgttgg cgatgccgcc cgcagtctcg agcgcgctgg cttcgggcga atagcggatg
     3361 ctcgcaccga actggctgcc gtcgcccagc gcggcttcga tctgatggcc gagatgggca
     3421 tggttgatca ccagttcggt gatcccggcg cgcaccagtt gctcgatgtg ccacacgatg
     3481 atgggtttgc caccgaccgg cagcagcggc ttgggcgtgt ggtcggtgag cgggcgcatg
     3541 cgttcgccgc ggcctgcggc caggatcatg gctctcaaaa gctgtaccct tcctgcagtt
     3601 cgcgcggctc cagttcttcc agcaggttca gcagcggctt gaggtcg
//
LOCUS       DASUIV010000218         8444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1076363, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000218 DASUIV010000000
VERSION     DASUIV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076363"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFW59_07920"
     CDS             complement(<1..612)
                     /locus_tag="VFW59_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364183.1"
                     /translation="MTNKGRILAVDDTPESLRLLTDILKAEGYEVRAAISGELALHAA
                     ALNPPELILLDIRMPGMNGYEVCRSLKAQPATRDVPVIFVSAASETSEKVEGFAVGGV
                     DFVTKPYQRDELLARVRTHLQLNRLSNHLEELVEERTAELRVSERKLKASLLESISAL
                     AATVELRDPYTAGHQRRTAELAVAIAGELQLTAEQTEGIHLAAI"
     gene            complement(609..3152)
                     /locus_tag="VFW59_07925"
     CDS             complement(609..3152)
                     /locus_tag="VFW59_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364184.1"
                     /translation="MALVILVASVSIGVNLLNYLYLSREAATLYDNKSLEYAANLHGM
                     LEPALWEVDDSLIQRIGDAFSANPEIALLIVRDEKQRVIYRYEKPNAVQTNRLIAVQH
                     NQQDIGSVELGLSLAAYEDKDRQLLLNSLITSAILILALLAASRWLLSSRLKEPIGAV
                     IDATREIVEGKYQQKEIPETYVEFTPILLAQKNMSEAIASREESLRLLNENLVTEIIE
                     RKLATEALRESEERWQFALEGAGDGVWDWNIQTDQTIFSKRWKAMIGYAEDEFDNSFE
                     TWKQHIYPDDLEPTLAAIQDYFDGRSQAYAVEFRMRCKDGSLKWILARGKIVERDEHG
                     RPLRMIGTHSDISGRKQAEEELRRYKDHLEEEVQLRTADLMLARNAAETANQAKSVFL
                     ASMSHELRTPMNAILGFSALMRKDPLLSPEQRDTLDIIKRSGEHLLNLINDVLEVAKI
                     EAGRVQLESAPFDLGVLVRDVIDMMQIRAQQKGLQLLLDQSSEFPRYINGDEARLRQI
                     LINLLGNAVKFTQQGGVTLRLGTRENSYSHLLIEVEDSGMGIKAEDQERIFQPFVQVG
                     TPSDQKGTGLGLTLTRQFVQLMGGTISVESEPGRGSLFRLDLPLHPAAESEIIESTRA
                     IKDEIVGLAPGQPGYRILIVEDQLENQLLLKKLMKDIGIQVRVADNGQQALDAFLEWR
                     PDLIWMDRRMPVMDGIEATRQIRKLPGGKDVKIVAVTASAFAEQRSEMLDAGMDDFVR
                     KPYRFNEIYECLTRLLGVQYTYAETSPQEEPAPLALTAEMLTALPQDLRHELRDALES
                     LEAERISSVIQRVAPLDAQLHKTLSQLAGRFDYPAILKTLQTNQTKDSA"
     gene            complement(3215..4039)
                     /locus_tag="VFW59_07930"
     CDS             complement(3215..4039)
                     /locus_tag="VFW59_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364185.1"
                     /translation="MNSATARMKALGLALLLSLAMPGMVRAAVAPVVFQSTETPPFWS
                     ASLPDNGLGGELLSLLSEAAGLPHRLEYLPVARFRSSLAPYIVGDPDILISKEHRAIF
                     PLCVFRAAFFFYKPHHEALEFRGIESLKGHTMGVLRGTVDDRDYFIRHGISIEESDSN
                     ESLLKKLHKGRLDFAILVDMTGIYTIKKLFPNEQDNFAQAAIPGSARPIAAMVDLSAP
                     EGRAIAARYRQVINKTLRSQKHRDILERYLGAGKIPPDTFEILEKYIKFYETTWAE"
     gene            complement(4036..4629)
                     /locus_tag="VFW59_07935"
     CDS             complement(4036..4629)
                     /locus_tag="VFW59_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364186.1"
                     /translation="MKNYKIPGFSSAWKVIGRLLAVSLLLYSWSAEATAPRITPKLFG
                     EETQTDILRYRLEQSASAPQSEGELLAELATEAFKAAGAAPVLDVQPSKQLAKYALFN
                     NEAMALIGSPQDFSAKEKKSYRLVTFYLRGGTSDGAPVAMIFGKRARADELYKSFNKG
                     MQAILKSGKYQEILEKHHVKIPSDYAGRLTRLNPAWR"
     gene            complement(4647..5975)
                     /locus_tag="VFW59_07940"
     CDS             complement(4647..5975)
                     /locus_tag="VFW59_07940"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5364187.1"
                     /translation="MKRFILPFLLAFSLLAPPVAAEDIMVVKPEEVGLSSPRLERISD
                     LIRSHVDQRKMAGAVVLVARHGKIAYFETFGQADEGKPMRRDTMFRICSMTKPVVTLA
                     ILQLYEEGKLLLSDPVAKYIPEFSHPKVVEMLPPGSNPAFRLVPAKRDITIKDLLTHT
                     AGMPYPFASEWYPQDRVLRQMHVLYEEAGISSGMYETEGTIGDMVKRLARLPLASQPG
                     EAFIYGMAADVQGYLVEVVSGMKLDDYIAEHIFKPLKMNDSYFFVPESKQGRMSAVWK
                     SDWQGKLEKVSPGPHREGDYVYSPTFQTQGPKTFLSGGVGMVTTPYDYFRFAQMLLNK
                     GELDGVRLVSRKTVELMTTNQIGKLSESTLHDEGWKFGLGLGLQVDREHNVDAGDVGT
                     FEWAGLYSTRFSVDPKEEKITIFLSQTHPFNHHFDLWDKLLVLSSSSIAD"
     gene            complement(6139..6849)
                     /locus_tag="VFW59_07945"
     CDS             complement(6139..6849)
                     /locus_tag="VFW59_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEX5364188.1"
                     /translation="MKKLLTPILFAAALLSQPVHAGENEIRQSLQSKLPKIGKIEHIV
                     KTPYSGLYEVVIGDQLLYTDAKGEYLFDGNVIDLKTRSNLSEARRQQLFAIDFDKLPL
                     DIALKRVKGDGSRRMAYFTDPNCGYCKKLEKELSKVSNVTLYLFIGPIFSPASAETVR
                     NIQCAKDPLKTWDDLMLNGNAPAQASCATKTDRVMALAKKMGVNGTPNLVFGNGMQAP
                     GYMPAEELEKQLNDSRKK"
     gene            complement(6867..8039)
                     /locus_tag="VFW59_07950"
     CDS             complement(6867..8039)
                     /locus_tag="VFW59_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HEX5364189.1"
                     /translation="MNYDIVIIGGGLVGASLAASLRHSGLSLALIESQHSPSLPAAGD
                     LGNWDSRIYAISPGSRRFLEEAGAWAHLDQSRVAPVEQMRVFGDADAKLEFSAYQMGT
                     AELACILENRALQHALWQVLQQQDNLTLLHPARCASLTVGEDAAELTLQDGRNIKAKL
                     VVGADGRDSWVRNQVGISAAPVDYQQHGVVANFSVEHPHRNIAWQWFQPDGILALLPL
                     PGNRVSMVWSVRPEKSAELLALTHEALCDQVSAAAQHTLGEMKVITQAAAFPLRLLVL
                     PHIAESRIALIGDAAHNMHPLAGQGVNTGFRDARQLTNILLERGGCSDCGDAQLLRRY
                     DRKRKEDIYSMQATTYGLKHLFNNDDPLLRKLRNLGMDATNYLTPLKKLLVQHALN"
     gene            8113..>8444
                     /locus_tag="VFW59_07955"
     CDS             8113..>8444
                     /locus_tag="VFW59_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX5364190.1"
                     /translation="MKIGRYQLKNNLIVAPMAGVTDRPFRMLCKRMGAGMAVSEMVAS
                     NSLLYGSEKTKRRANHEGEVDPISVQIVGADPKMLAQAARYNVDHGAQIIDINMGCPA
                     KKICNVMAG"
BASE COUNT         1566 a         2451 c         2495 g         1932 t
ORIGIN      
        1 gatggcggcc agatggattc cttcagtctg ctctgccgtg agctgcagct ccccggcgat
       61 ggcgaccgcc agttcggcgg tgcgacgctg atggccggcc gtataggggt cgcgcaactc
      121 aaccgtggcc gccagtgcag aaatggattc gagcaggctg gcctttagct tgcgttcact
      181 gactctcagt tcggcggttc gttcctcgac cagttcttcc agatggttgc tcagacggtt
      241 cagttgcaaa tgggtgcgga ctctggccag caattcatcg cgctgataag gcttggtgac
      301 gaaatccacg cccccgactg cgaagccctc tactttttcg gacgtttcac tggcggcgct
      361 gacgaagatc acgggtacgt cgcgcgttgc gggctgtgcc ttcaggcttc ggcacacttc
      421 atatccgttc atcccgggca tgcgaatatc gagcaggatc aattcgggcg gattcaaggc
      481 ggctgcgtgc aaggccagtt cgccgctgat cgcggcacgc acttcataac cttccgcctt
      541 caggatgtca gtcagcagcc tcaatgattc cggcgtgtca tcgactgcga gtatccttcc
      601 tttgtttgtc atgcagaatc tttcgtctgg tttgtctgca aggttttcag gatagcagga
      661 taatcgaaac gccccgccag ttgtgagagc gtcttgtgca attgcgcatc gagcggcgcg
      721 acgcgctgga taacggagct gatgcgttcg gcctcaagcg attccagggc atcgcggagt
      781 tcgtggcgca aatcctgcgg cagtgccgtc agcatctccg cagtcaatgc caaaggagcg
      841 ggttcttcct gcgggctggt ctcggcatag gtatattgca cacccagcag cctggtcagg
      901 cattcataga tctcgttgaa tcggtagggc ttgcgcacga agtcgtccat gcccgcgtcg
      961 agcatttcgc tgcgctgttc cgcgaaggcc gatgcggtaa ctgcgacgat cttcacgtcc
     1021 ttgccgccgg ggagcttgcg gatttgtcgc gttgcttcga tgccgtccat cacgggcatg
     1081 cgcctgtcca tccagatcag atcaggccgc cactccagaa atgcatccag ggcctgttgg
     1141 ccgttgtcgg ccactctgac ctgaatgcca atatccttca tgagtttttt gagcaatagc
     1201 tgattctcca actgatcctc gacgatcagg atgcgatatc ccggctggcc gggagccagt
     1261 cccacaatct cgtcctttat tgctcttgtc gactcgatta tctcggattc ggcggcggga
     1321 tgcagcggca gatcgaggcg gaagagggag cctcggcccg gctcgctctc tacgcttatc
     1381 gtgccgccca tcagttgcac gaactgccgg gtgagcgtca atcccagacc ggtgcccttc
     1441 tggtcgctgg gcgtcccgac ttgcacgaag ggctggaaaa ttctttcctg gtcttccgcc
     1501 ttgatgccca tgccggaatc ctcgacctcg atcaacaggt gggagtatga attttctcgc
     1561 gtgcccaggc gcagcgtaac gccgccttgc tgggtgaatt tgaccgcatt gccaagcaaa
     1621 ttgatcaata tctgacgcag ccgcgcctcg tctccgttga tatagcgggg aaactcggaa
     1681 gactggtcca gcagcagttg caagcctttc tgctgagcgc ggatttgcat catgtcgatg
     1741 acgtcccgga ccagcacgcc gagatcgaat ggggcgctct ccagttgcac ccggcccgct
     1801 tcgatcttgg ccacttccag cacatcgttg atcaggttca acaggtgttc gccgctgcgt
     1861 ttgatgatgt cgagggtgtc gcgctgctct ggcgatagca atgggtcttt acgcatcagg
     1921 gcggagaagc cgagtatggc attcatcggc gtgcgcaatt cgtggctcat gctggccaga
     1981 aatacgctct tcgcctgatt ggccgtttcg gccgcattgc gcgccagcat cagatcggcg
     2041 gtgcggagct gcacttcctc ctccagatgg tccttgtatc gccggagttc ttcttctgcc
     2101 tgcttccgtc cggaaatgtc ggaatgcgta ccgatcatgc gcaatggccg gccatgctca
     2161 tcgcgctcca cgatcttgcc gcgtgcaagt atccatttca ggctgccatc cttgcagcgc
     2221 atgcggaatt cgacggcata agcttgtgac ctgccgtcaa aataatcctg tatcgcagcc
     2281 agcgtcggtt ccagatcgtc gggatagata tgctgcttcc aggtctcgaa gctattgtcg
     2341 aattcgtcct cggcatagcc gatcatggcc ttccagcgtt tgctgaagat cgtctgatcg
     2401 gtttgaatat tccagtccca cacgccgtct ccggcaccct ccagcgcgaa ttgccagcgc
     2461 tcctcgcttt cccgcaatgc ctctgtggcc agtttgcgtt caatgatttc cgtcacgaga
     2521 ttttcgttga gcaggcgcaa gctctcctct cggcttgcaa tggcctccga catgtttttt
     2581 tgggcgagca gaataggggt gaattccaca taggtttcag gaatttcttt ttgctgatat
     2641 ttgccttcga cgatctcccg tgtcgcgtcg atcaccgcac ctatgggctc tttcagtcgg
     2701 gatgacagca accatcgcga agccgccagc agagcgagaa tgagaatcgc cgatgtgatc
     2761 aggctgttta gcaacagctg cctatccttg tcctcatagg cggctaatga cagtccgagt
     2821 tcgacactgc caatatcctg ttggttgtgc tgaacggcaa tcaggcggtt cgtctgaact
     2881 gcattgggct tctcatagcg atagatgacc cgctgcttct catcgcggac gattaacaag
     2941 gcgatttcag ggttggccga gaaggcgtca cctatgcgct ggatcaggct gtcgtcgact
     3001 tcccagagcg caggttcgag catcccgtgc aggttggctg catactccag cgacttgttg
     3061 tcatagaggg tcgccgcctc gcgtgagagg taaaggtaat tgagcagatt gacgccgatc
     3121 gataccgacg cgaccagtat caccagagcc attctgagat cgcggtaaag tgacgtttgc
     3181 ttcccggatg acggttccat attgttccgc cgaattattc ggcccaggtg gtttcgtaaa
     3241 atttgatgta tttttccaat atctcaaacg tatcgggcgg gattttcccg gccccgagat
     3301 agcgttccag aatatcgcga tgtttctggc tgcgcagcgt tttattgatg acctgacggt
     3361 aacgcgctgc aatggctctt ccctccggcg ccgacagatc gaccatggcc gcgatcggac
     3421 gcgccgaacc cggaatggca gcctgagcaa aattatcctg ttcattgggg aagagttttt
     3481 tgatcgtgta gatgccagtc atgtctacca gtatcgcgaa atcgagcctc cccttgtgca
     3541 acttcttcag cagcgattca ttcgagtcgc tctcttctat gctgatgcca tgcctgatga
     3601 aataatcccg gtcgtcgact gtaccgcgca atacccccat ggtgtgccct ttcaggctct
     3661 cgatgccacg gaattcgagc gcctcatggt gcggcttgta aaaaaagaag gccgcgcgga
     3721 acacgcacaa gggaaagatc gcacggtgtt ccttggatat caggatatcg gggtcgccga
     3781 cgatatacgg ggcaaggctg gatcggaaac gtgccaccgg caaatattcg agcctgtgcg
     3841 gcaggccggc cgcttccgat aacaggctca acagttctcc gcccaagccg ttgtcaggca
     3901 atgaggccga ccagaatgga ggggtttcgg ttgactgaaa aaccacgggt gcgaccgctg
     3961 cccgcaccat tcccggcatt gccagcgaca gcagcagtgc cagacccaat gccttcatgc
     4021 gcgcggttgc ggagttcatc tccaggccgg attgagccgc gtcaaacgcc ctgcataatc
     4081 ggacggaatc ttgacgtggt gcttctccag tatctcctga tactttccgc tcttgaggat
     4141 cgcctgcatg cccttgttga aggatttata cagttcgtct gcgcgcgccc gcttgccgaa
     4201 tatcatcgca accggcgccc cgtctgaggt gcctccccgc agatagaacg tcaccagacg
     4261 gtagcttttc ttttctttag ccgaaaaatc ctgcgggctg ccgatcagcg ccatcgcctc
     4321 gttgttgaac agcgcgtact tggccagctg cttggacggt tgcacatcca gcacaggggc
     4381 tgcccctgcc gccttgaacg cctcggtcgc gagctccgcg agcaattccc cttccgactg
     4441 cggcgcggat gcagactgtt ccagacgata tctcaggatg tcggtttggg tttcttcgcc
     4501 gaagagtttc ggcgtgatgc gcggagcggt cgcctccgcg ctccacgaat acagcagcag
     4561 gcttactgcc agcaagcgcc cgatgacttt ccatgccgag gagaagccag gaattttgta
     4621 atttttcatg atgagcctcg cgattatcag tctgcaatac tggaactgga cagcaccagc
     4681 aacttgtccc acaggtcgaa gtggtgattg aaaggatggg tctgcgacag gaagatcgtg
     4741 atcttctctt ctttcggatc gacgctgaag cgcgtggaat aaaggcccgc ccattcgaat
     4801 gtgccgacat cgcccgcatc cacattgtgt tcacggtcta cctgaagccc caggcccagt
     4861 ccgaatttcc agccttcgtc atgcagcgtg ctctccgaga gttttccgat ctgattggtc
     4921 gtcatcagtt cgacggtctt gcggctgacc agacgcacgc catccagctc gcccttgttc
     4981 agcaacatct gggcgaaacg gaaatagtcg tagggcgtcg taaccatgcc gacgccgccc
     5041 gagagaaatg tcttcggtcc ctgggtctgg aaggtcgggg aatagacata gtccccttcg
     5101 cgatgcgggc caggggacac tttttccagc ttgccttgcc agtcgctctt ccacacggca
     5161 gacattctgc cctgcttgct ttccggcacg aagaaatagc tgtcgttcat cttcagcggc
     5221 ttgaagatat gctcggcgat gtagtcgtcg agtttcatgc cggagaccac ttcgaccagg
     5281 tagccctgaa cgtccgccgc catgccgtag atgaacgcct ctccaggctg gctggccagt
     5341 ggcaggcggg ccagccgctt gaccatgtct cctatcgtcc cttcggtctc gtacattccg
     5401 ctgctgatgc cggcctcctc gtatagcaca tgcatctggc gcagcacgcg gtcctggggg
     5461 taccactcgg acgcaaatgg gtaaggcata cctgcggtgt gggtcagcag atccttgatc
     5521 gtgatatccc gcttggcagg aaccagcctg aaggccggat tggagcccgg cggcagcatc
     5581 tcgacgacct tgggatgcga aaattccgga atgtatttgg cgacaggatc ggacagcagc
     5641 agcttgccct cctcgtacag ctgaaggatc gccagggtga ccacaggctt ggtcatcgag
     5701 cagatacgga acatcgtgtc cctccgcatc ggcttgccct catcggcttg cccgaacgtt
     5761 tcgaagtagg cgatcttgcc atgccgcgcg accagcacga ccgctccggc catttttctc
     5821 tgatcgacat ggctgcggat caggtcgctt attctttcca gacgcgggga ggacaacccg
     5881 acctcttccg gtttgaccac catgatgtcc tcggccgcga cgggtggggc gagaagagaa
     5941 aaggccagca agaacggcag tatgaagcgc ttcatggtca cctcctgtgg ggcgttcgcc
     6001 cattcactgc gcgaacggag tgtgaatact accagcgttc tccttggatc ggcataggac
     6061 tgtcctgatg ggcgcgggtg ctgaattccg gctgtgccgc aggatccgcg ccccggctct
     6121 tgagccgcgc tttagcgctt atttcttccg cgagtcgttc agttgttttt ccagttcttc
     6181 ggcgggcatg tagccggggg cttgcatgcc gttgccgaat accagattgg gggtgccgtt
     6241 cacgcccatc ttcttggcga gcgccatcac tctgtcggtc ttggtcgcgc aggatgcctg
     6301 ggcgggtgca ttgccgttga gcatcaggtc atcccaggtt ttgagcgggt ctttggcgca
     6361 ctggatgttg cgcacggtct ctgcggatgc gggcgagaag atcgggccga tgaacaggta
     6421 gagcgtcacg ttggatacct tgctcagttc tttctccagt ttcttgcagt agccgcagtt
     6481 cggatcggtg aaataggcca tgcggcgcga gccgtcgccc ttgacgcgct tgagagcgat
     6541 gtccagcggc agcttgtcga agtcgatggc gaacagttgc tggcggcgcg cttcggatag
     6601 gttgctgcgg gtcttcaggt cgatcacgtt gccgtcgaac aggtattcgc ccttcgcgtc
     6661 ggtgtacagc agctggtctc cgatcacgac ttcatagaga ccggagtagg gggtcttgac
     6721 gatatgttca atcttgccga tcttcggcag cttgctttgc agcgactggc ggatctcgtt
     6781 ttctccggcg tggacgggct gtgacagcag ggccgcagcg aacaggatgg gggtgagcag
     6841 tttcttcatg tcttgccttt aatcgtttaa ttgagtgcgt gttggaccag tagcttcttc
     6901 agcggggtca ggtagtttgt ggcatccatg ccgagattgc gcagcttgcg caacagcgga
     6961 tcgtcgttgt tgaacaggtg tttgaggccg taggtggtgg cctgcatcga gtagatgtct
     7021 tccttgcgtt tgcggtcgta gcggcgcaac agctgcgcat cgccgcagtc gctgcaaccg
     7081 ccgcgctcca gcaggatatt ggtcagctga cgcgcatcgc ggaagccggt gttcacaccc
     7141 tgcccggcca agggatgcat gttgtgcgcg gcatcgccga tcagcgcgat gcgcgattcg
     7201 gcgatgtgcg gcagcaccag caggcgcaac gggaaggcgg cggcttgcgt gatgaccttc
     7261 atctcgccca gcgtgtgctg tgcggcggcg gaaacttgat cgcagagcgc ttcgtgagtg
     7321 agcgccagca actcggcgga tttctccgga cgcaccgacc acaccatgga tacgcgattg
     7381 cccggcagcg gcagcagtgc gaggatgccg tccggctgga accattgcca ggcgatgttg
     7441 cgatgcgggt gttcgacgct gaagttggcg accacgccgt gctgctggta gtccaccggt
     7501 gccgcgctga tgccgacctg attgcgcacc caggagtcgc ggccgtccgc gccgaccacc
     7561 agttttgcct tgatgttgcg accgtcctgc agcgtgagtt cggcggcgtc ttcgcctacg
     7621 gtcagcgagg cgcaacgcgc cggatgcagc agcgtgagat tgtcctgttg ctgcaatacc
     7681 tgccacagcg cgtgttgcag cgcgcggttc tccagtatgc aggccagttc ggcagtgccc
     7741 atctggtagg cgctgaattc cagcttcgca tcggcgtcgc cgaataccct catttgttcc
     7801 acgggtgcga cgcgactttg atcgagatgg gcccaggcgc ccgcttcctc gaggaagcgg
     7861 cggctgccgg ggctgatcgc gtagatgcgg ctgtcccagt tgccaaggtc accggcggct
     7921 ggcagcgaag gtgagtgctg gctctcgatc agcgccagcg acaggccgct gtgcctgagc
     7981 gaagcggcca gactggctcc aaccaggccg ccgccgatga tgacgatgtc gtaattcatg
     8041 gtgcgtcatc atagcgagat ttccccacgc tgtccgcttg ctttataatc accgccccct
     8101 tcgagaaaca tcatgaaaat cggtcgctac caactcaaga acaacctgat cgtcgcaccc
     8161 atggcgggag tgaccgatcg gcctttccgc atgctgtgca aacgcatggg cgcgggcatg
     8221 gcggtgagcg agatggtggc ctcgaattcg ttgctgtacg gctcggagaa gaccaagcgc
     8281 cgcgccaacc acgagggcga ggtcgatccg atatcggtgc agatcgtcgg ggccgatccc
     8341 aagatgctgg cgcaggccgc gcgctacaac gtggaccacg gcgcgcagat catcgacatc
     8401 aacatgggtt gtccggccaa gaagatatgc aacgtgatgg cggg
//
LOCUS       DASUIV010000219         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1263369, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000219 DASUIV010000000
VERSION     DASUIV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263369"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /gene="rdgC"
                     /locus_tag="VFW59_07960"
     CDS             complement(<1..226)
                     /gene="rdgC"
                     /locus_tag="VFW59_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEX5364191.1"
                     /translation="MWFKNLLVYRLNEWPVTPDALEEKLSQHILQACSGLEMQRHGWV
                     SPKADGEPLVHALGQQLLISLGTEKKLLPST"
     gene            440..2449
                     /locus_tag="VFW59_07965"
     CDS             440..2449
                     /locus_tag="VFW59_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5364192.1"
                     /translation="MLSKLNSVQRDAASYLGGPLLVLAGAGSGKTRVITHKIAYLVEE
                     CGYEARNIAAITFTNKAATEMRERVGTLLPGSRSKGLVVSTFHSLGMNILRAEAGLLG
                     YKPKFSIFDSSDSWKILSELTNSGDKQEIRDLQTQISNWKSAFISPAQAEAQVEDDLA
                     KVQARVYARYQETLRAYQAVDFDDLIHLPVVLFKEHPEALARWQHRLRYLLIDEYQDT
                     NDCQYQLTRLLAGVRAAFTAVGDDDQAIYAWRGASIENLHNLRNDYPSLRVIKLEQNY
                     RSSQRILKVANHLINHNTKVFEKKLWSDHGIGDPIRIYAARDDEQEAESVVVKLLAHK
                     FENRTKFSDYAILYRSNYLARTFEEQLRAQRVPYTVSGGTSFFDKAEIKDIISYLRLI
                     ANPDDDPAFIRAITTPKRGIGNATLEKLGSYAGTRKISLFEAAFEPRMAEELPPRQHE
                     DLMMFCKFINRIGDRAEADPCGELLDELLRAINYETWLYDSHESRQAEAKWKNVEDFI
                     GWIKRKAEADEKSLIAMVQTIALINMLEGKDEEPDAVTLSTLHAAKGLEFPHVFIIGV
                     EEDILPFRDSDEKGIEEERRLMYVGVTRAERTLQISYCNRRKRGKDWSACEPSRFLDE
                     MPQEELVYAGAHADAAPTVTKDEGMDKLARLKAMLNRPVEDNGNG"
     gene            2436..3284
                     /locus_tag="VFW59_07970"
     CDS             2436..3284
                     /locus_tag="VFW59_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5364193.1"
                     /translation="MATAEMTAARPDVAEGEPAELRQAALFWLAERDAARKAAGVRRL
                     AQAWAAGGIELDAEAVLTAHQTIPGHPEKPELVAPRLVKHRSMITPEGRAILIHALVH
                     IEFNAINLALDALWRFAAMPRDYYADWLQIADEEALHFSLLAEHLRGQGYAYGDFPAH
                     NSLWEMAVKTRHDVLARMALVPRTMEARGLDVVPSLRDKLAQAGDKAAAAIMDIILRD
                     EIGHVAIGNRWYGHLCEQRGLEPVATYARLAAEHQAPLLRGPFNLEARRAAGFSEPEL
                     VALRQR"
     gene            3545..3868
                     /locus_tag="VFW59_07975"
     CDS             3545..3868
                     /locus_tag="VFW59_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5364194.1"
                     /translation="MRVFNLLTHQEICRELGQRCRRLRLQANLSQLELAERTGVSLSS
                     IRRLEASGQSTLELLVRVTQALHLTNQLESLLVLPVLSIADAERNAATDMRQRAASTR
                     RKKSA"
BASE COUNT          812 a         1187 c         1254 g          708 t
ORIGIN      
        1 cggtcgaggg aagcagcttc ttctcggtgc cgaggctgat caataattgc tggccgagcg
       61 catgcaccag cggctcgcca tcggccttgg gcgagaccca gccatggcgt tgcatttcca
      121 ggccggagca ggcttgcagg atgtgttgcg agagtttttc ttcgagggcg tcgggagtga
      181 ccggccattc attgagccga tagaccagca gattcttgaa ccacataaat tgagaccgga
      241 gcagagtaaa aaggctggcg attctatcgt ttcgtctggt cgtattccag cggtctttct
      301 gttttttgct cagtggcctc cgttactggc gcatggagga agcccggaag cgacgggcgg
      361 ggttttcggt caggctttgg tatcatgcac gcctttcgcg acacatctcc cccgtattca
      421 gtatgcccgg tatttcagta tgttaagcaa gctcaattcc gtgcagcgcg atgccgcgtc
      481 ctacctcggc ggcccgttgc tggtgctggc cggggcgggc agcggcaaga cgcgcgtcat
      541 cacccacaag atcgcctatc tggtcgagga gtgtggctat gaggcgcgca acatcgcggc
      601 gatcaccttt accaacaagg cggccaccga gatgcgcgag cgcgtcggca cgctgctgcc
      661 tggcagccgg tccaaggggc tggtggtcag caccttccat tcgcttggga tgaacatcct
      721 gcgcgccgag gccgggctgc tgggctacaa gccgaagttt tccatcttcg attccagcga
      781 cagttggaag atattgagcg agctgaccaa ctccggcgac aagcaggaaa tccgcgactt
      841 gcagacgcag atctcgaact ggaagtcggc cttcatctcg cccgcgcagg ccgaagcgca
      901 ggtggaggac gacttggcga aagtgcaggc gcgcgtttat gcccgctatc aggagacgct
      961 gcgcgcctat caggcggtgg acttcgacga cctgatccac ctgccggtgg tgctgttcaa
     1021 ggaacatccg gaggcgctgg cgcgctggca gcaccggctg cgctatctgc tgatcgacga
     1081 atatcaggac accaacgatt gccagtacca gttgacccgg ctgctggccg gtgtccgtgc
     1141 cgcgttcacc gccgtgggcg acgacgatca ggcgatctat gcctggcgcg gcgcgagtat
     1201 cgagaacctg cacaacctgc gcaacgatta tcccagcctg cgcgtgatca agctggagca
     1261 gaactatcgc tcgtcgcagc gcatcctcaa ggtggcgaat cacctgatca accacaacac
     1321 caaggtgttc gagaaaaagt tgtggagcga ccacggcatc ggcgacccga tccgcatcta
     1381 cgcggcgcgc gacgacgagc aggaggccga gagcgtggtg gtgaagctgc tggcgcacaa
     1441 gttcgagaac cgcaccaagt tttccgacta cgcgatcctg tatcgcagca attatctggc
     1501 gcgcaccttc gaggagcagt tgcgcgcgca gcgcgtgccg tacaccgtca gcggcggcac
     1561 atcattcttc gacaaggccg agatcaagga catcatctcc tacctgcggc tgatcgcgaa
     1621 cccggacgac gatccggcct tcatccgcgc gatcaccacg ccgaagcgcg gcatcggcaa
     1681 cgccacgctg gagaagctgg gcagctatgc cggtacgcgc aagatcagcc tgttcgaggc
     1741 ggcgttcgag ccgcgcatgg ccgaagaact gccgccgcgc cagcacgaag acctgatgat
     1801 gttctgtaaa ttcatcaacc gcatcgggga tcgcgccgag gccgatccgt gcggcgagtt
     1861 gctggacgag ctgctgcgcg cgatcaacta cgagacctgg ctgtacgact cgcacgagtc
     1921 gcggcaggcc gaggccaagt ggaaaaacgt cgaggacttc atcggctgga tcaagcgcaa
     1981 ggccgaggcg gacgagaaat cgctgatcgc gatggtgcag accatcgcgc tgatcaacat
     2041 gctggaaggc aaggacgagg agcccgacgc ggtcacgctg tccacgctgc atgccgccaa
     2101 ggggctggag ttcccgcatg tgttcatcat cggcgtggag gaggacatcc tgccgttccg
     2161 cgacagcgac gagaagggca tcgaggaaga acgccgcctg atgtacgtcg gggtcacccg
     2221 cgccgaacgc accctgcaaa tcagctactg caaccggcgc aagcgcggca aggactggag
     2281 cgcctgcgaa ccgagccgct tcctcgatga aatgccgcag gaggaactgg tctatgccgg
     2341 ggcccatgcg gatgccgcgc ccaccgtgac caaggacgag ggcatggaca agctggcgcg
     2401 gctgaaggcg atgctgaaca gaccggtgga agataatggc aacggctgaa atgaccgccg
     2461 ctcgccccga cgtggcggag ggtgaaccgg cagagttgcg gcaggccgcg ctgttctggc
     2521 tggcggaacg cgatgccgcg cgcaaggccg caggcgtgcg tcgtctggcg caagcctggg
     2581 ctgcgggcgg gatcgagctg gatgccgagg ccgtattgac ggcgcaccag actattcccg
     2641 gtcatccgga aaagcccgag ctggtcgcac cccgtctggt caagcaccgc tcgatgatta
     2701 cgccggaggg ccgcgcgatc ctgatccatg cattggtgca tatcgagttc aacgcgatca
     2761 atctggcgct ggatgcgctc tggcgttttg cggccatgcc gcgcgactat tacgccgact
     2821 ggctgcaaat cgcggacgag gaggcgctgc atttctcgtt gctggccgaa cacctgcgag
     2881 ggcagggcta cgcctacggc gatttccccg cgcacaacag cctgtgggaa atggcggtca
     2941 agacgcggca cgacgtgctc gcgcgcatgg cgctggtgcc ccggacgatg gaggcgcgcg
     3001 ggctggacgt cgtgccttcg ctgcgcgaca agctggcgca ggcgggcgac aaggctgcgg
     3061 cggcgatcat ggacatcatc ctgcgcgacg agatcggcca tgtcgcgatc ggcaaccgct
     3121 ggtacggcca cctgtgcgaa cagcgcggtc tggagccggt tgccacctac gcgcggctgg
     3181 ccgccgaaca tcaagcgccg ctgctgcgcg gcccgttcaa tctggaggcc cggcgcgctg
     3241 cgggattcag cgagccggag ttggtcgcgt taaggcaacg ctgattaagt cgtcagtccc
     3301 gcgaaggcgg gaatctagct tgttgattta acagggttcc cgccttcgcg ggaacgacaa
     3361 aatcatactt gttcagcctt accttaaagt gaaattcgcg tggacagccg ccggtcgttt
     3421 ttcggtgtga gataccgagg catgctgtcc cgaccaaacg ccccccataa acttgcgatc
     3481 cagcacgcaa aacaagaatt caaaaaagag cagttaaagt aagtttattg atacaatcgt
     3541 tcatatgaga gtatttaatc tgctaaccca tcaggaaatc tgccgcgaac taggccagcg
     3601 ttgtcggcgt ttgcggttgc aggcaaatct cagccaactc gaactggccg aacgcacagg
     3661 cgtttcgctt tcctccatcc gccgtttgga agccagcggg caatccacgc tggaactgct
     3721 ggtacgggta acccaggctt tgcacctcac gaaccagttg gagagcctgc tggttttgcc
     3781 cgtactcagc atcgccgatg ccgagcgcaa tgccgctacc gacatgcgcc aacgggctgc
     3841 gtcgacgcgc cgcaaaaaat ccgcatgaaa aaactacagg tcatctatgc aggctggggc
     3901 gaacgctgga cgctcggcac actggcagat accggccagc acattctgtt tgaatattcc
     3961 c
//
LOCUS       DASUIV010000220         6376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_333393, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000220 DASUIV010000000
VERSION     DASUIV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333393"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFW59_07980"
     CDS             <1..1307
                     /locus_tag="VFW59_07980"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5364195.1"
                     /translation="GAINSYFRAYHFSNVAYDRSLFRAALALADQVEVVQGKVVVDMP
                     QKAIDLLEYDKDDWVYYRVTDPQGHTIAGEDDLALPAPLPVPGGHQYFDSTLGDKVVR
                     VVAFSLPLAGTSAQGSVLVQVAETKSKRDQLADEIITSMMLPQILIVLFSGFMVYFGV
                     RWGLSSLDRLQRAIESRSHLDLNAIPIEGSPREVHPLLHSMNDLLRRLQASMVQQQRF
                     TADVSHQLRTPLAGIQTQAEMALRETDPVRIHHALEWIRSGTVRLSHLVNQLLALARV
                     EVGADRNVDLHELDLVQLARDTTSEWVARALNRQIDLGFEATRGEVLIRGNLLLLQEL
                     ISNLLDNAIRYTPVRGKITVTVDVDENGAILKVEDSGPGIPVAEREHIFERFYRSQES
                     AGCGLGLAIVREIALIHHAQASVESGADGRGTQVRIQLPTSR"
     gene            complement(1379..2251)
                     /locus_tag="VFW59_07985"
     CDS             complement(1379..2251)
                     /locus_tag="VFW59_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364196.1"
                     /translation="MQLAHITTRPPHLVPALEALARRADNQEAAQTLLDELSGITVLV
                     ARKFSNERTADGLLEAFHLTTGCISLAISQANLPEDADAHLSFLLHHGAEHVFQMGFR
                     HIKALSALPYSAFISDFDRDPQIQQRDIKLLFAEICRADPIAPWSGDTLYNHELLDRQ
                     NIQKIVECAQWLRKKHYAGPVKSRDMDAHAVIGIAIIFAILGDGRIVARTGQEEIESL
                     IQRARKTRPDIDAGWNELLNKIPAEYQPILRERMDEYRNTIIKKILSKTSAKTVLIEI
                     QKNYAGDEQEVDYE"
     gene            complement(2284..3072)
                     /locus_tag="VFW59_07990"
     CDS             complement(2284..3072)
                     /locus_tag="VFW59_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364197.1"
                     /translation="MADDKNFPAISRQVTELVLAGSTEHAEEAINETAEQFGDLAVVT
                     ILDTLEPQVAAMHLAAFDGGKLSLATLLISPKAWAESLAFFAATWSEDMIEDEPEVLT
                     ESLFAHLHGVIFSSDDPERRAALIHEALSTDWGTTAFAVVFSTAIEDIIELANDIYSR
                     GPHSSGLTTSDHDVIPLALEIARSDADAWDRVLFEIFPDWQPGENQLRARSEEDDEEE
                     PHHDDALARTTHELLVRLRRQVPGKALGTPKKGARPSLGDDIFA"
     gene            complement(3065..3595)
                     /locus_tag="VFW59_07995"
     CDS             complement(3065..3595)
                     /locus_tag="VFW59_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364198.1"
                     /translation="MNKNIHHEFAYQSRELGVQIEDTLGDPPDRKNYARVIASLIGEY
                     AKGTEIDDVNMLSFALAEHLRFDDPAGLLAQCKQYDEGDAAKTVAMVSCADSEAAKAL
                     AAVSAKNPELARKAILTVFLFKNTKRWSEADESLEALEEEDNEKNWDDDIEDDERGVP
                     GQDVIDLFDKREDGNG"
     gene            complement(3608..3916)
                     /locus_tag="VFW59_08000"
     CDS             complement(3608..3916)
                     /locus_tag="VFW59_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5364199.1"
                     /translation="MANPYNILGVSPTATTEEIKKAYRSLAMRHHPDRNSHASSEVRF
                     NAIKTAYDLLSDPQKRAEYNQSLNNRIIIDPDNEARTLWLSLFDRCGLVVPQNHHQST
                     "
     gene            4125..5219
                     /gene="apbC"
                     /locus_tag="VFW59_08005"
     CDS             4125..5219
                     /gene="apbC"
                     /locus_tag="VFW59_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX5364200.1"
                     /translation="MSFTESDVQSALKSLIDPNTRKDFVSGKSVKNVKVSGNDVSLDI
                     TLGYPAKSVWAEIQTMVETHLKASLPGAGKISANVSSKVVPHAVQRGVKLVEGVKNII
                     AVASGKGGVGKSTTAVNLALALAAEGARVGMLDADIYGPSQPTMLGIEGRPKSRDGKS
                     IEPMEGHGLQAMSIGFMIEGTDAPMIWRGPMVTQALDQLLHQTRWDNLDYLVVDLPPG
                     TGDVQLTLAQKVPVTGAVIVTTPQDIALLDARKGLKMFEKVGIKIVGIVENMSTHICS
                     QCGHEEHIFGAGGGEKMCADYNTEFLGSLPLDIRIREQADSGAPTVVSDPDGNIARVY
                     RQIARRVAVKVAEMAQDHSAAFPKIVVQNT"
     gene            5246..5767
                     /locus_tag="VFW59_08010"
     CDS             5246..5767
                     /locus_tag="VFW59_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5364201.1"
                     /translation="MTIGGGKSSLAGLVIQGSGVASSGRIASMLENAHMFKDMEWAQI
                     EALSNYIQLYHADPNTVLFHEGDRGDFMCIVLEGKLEVRKEDAQRVDKTVSTVMPGRS
                     LGEMTMVDGEPRSATAVAVEESLLAVLTQENFKLIMHDRPALAVKLLYKIAQLISQRL
                     RQTSGMLVDYLEN"
     gene            complement(5764..>6376)
                     /gene="prmC"
                     /locus_tag="VFW59_08015"
     CDS             complement(5764..>6376)
                     /gene="prmC"
                     /locus_tag="VFW59_08015"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5364202.1"
                     /translation="EREFFGLNFKVTPATLIPRPDTELLVELALQKIPQQGRVLDLGT
                     GSGAIALSIAHARPDAEVIAVDASPEALEVAQDNARRLKLANVRLLHSDWFSALQDAR
                     FVLIVSNPPYIASADAHLEQGDLRFEPRTALASGSDGLDDIRRIIADAKAHLLADGWL
                     LFEHGYDQAERVRELLRQAGYAEVFSAHDLSGIERVSGGRAAF"
BASE COUNT         1204 a         1770 c         2046 g         1356 t
ORIGIN      
        1 taggcgccat caattcttat tttcgcgctt atcatttctc gaatgtggcc tatgaccgat
       61 cgttgtttcg cgccgcgctg gcattggccg accaggtaga agtcgtgcag ggcaaggtgg
      121 tggtggatat gccgcagaag gccatcgatt tgctggaata tgacaaagat gattgggtct
      181 attatcgcgt caccgatccg caaggccata cgatcgccgg cgaggatgac ctggctttgc
      241 ccgccccgct gcccgtcccg ggtgggcatc aatattttga cagcacactc ggtgacaaag
      301 tcgtccgtgt cgttgcgttt tcattgccgc tggcaggcac gagcgcacaa ggcagtgttt
      361 tggttcaggt ggccgagacg aaatccaaac gggatcagct ggccgatgaa atcattacat
      421 cgatgatgct gccgcaaatc ctgatcgtgc tgttttccgg atttatggtg tacttcggtg
      481 tgaggtgggg gctttcttcg ctggaccggc ttcagcgtgc catcgaatcg cgttcacacc
      541 tcgacctgaa cgcgataccg atcgaaggct cgccgcgcga ggttcatccc ctgctgcatt
      601 ccatgaatga cctgttgcgc cgcctgcagg ccagtatggt tcagcagcaa cgttttaccg
      661 ccgatgtctc gcatcagctc cgtaccccgt tggcgggcat acagactcag gctgaaatgg
      721 ctttgcgtga aaccgatccg gtgcgcatcc atcacgcgct ggaatggata cgctcgggaa
      781 cggtcaggtt gtcgcatctg gtcaatcagt tgctggcgct tgcacgggtc gaagtcgggg
      841 ctgacaggaa cgtggatctg catgagcttg atcttgtgca actggcgcgc gatacgacct
      901 ccgaatgggt ggctcgcgcc ttgaatcggc agatcgattt aggctttgag gcgactcggg
      961 gcgaggttct gatacgtggc aacctgctgc tgttgcagga gctgatttcc aatttgctgg
     1021 ataacgcgat tcgttatacg ccggtgcgtg gaaaaatcac ggtgacggtt gatgtggatg
     1081 aaaatggcgc gatccttaag gttgaagaca gcggccccgg cattcctgtc gcagagcgcg
     1141 agcatatctt tgaacgcttc taccggtcgc aggaaagtgc cgggtgcggg ctggggctgg
     1201 ctatcgtgcg cgagatcgcg ctgatacatc atgcacaggc gagtgtggaa agcggggcgg
     1261 atggccgggg cacgcaggtc aggatacaac tgccaacaag ccgctagatt accaggcggt
     1321 catacacagg gggcgttagt gagcggacag aaagcgtacg gtcaatgcgg tgcggagatc
     1381 actcgtagtc gacctcttgt tcatcgcccg cgtaattctt ctggatctcg atgaggactg
     1441 tttttgcgct ggttttagac aggatctttt tgatgatggt gttgcggtat tcgtccatgc
     1501 gttcgcgcag tatcggctga tattcggccg gaatcttgtt cagcagttca ttccagcccg
     1561 catcgatatc ggggcgtgtc ttgcgggcgc gctgaatcag gctctcgatc tcttcctgtc
     1621 cggtgcgcgc gacgatgcgc ccgtcgccca ggatcgcaaa aataatcgcg atgccgatga
     1681 cggcatgggc atccatgtca cggcttttga ccgggcccgc gtaatgtttt ttgcgcaacc
     1741 attgcgcaca ttcgacgatc ttctggatgt tctgccgatc cagtaattca tggttgtaga
     1801 gggtatcccc gctccacggg gcgatcgggt ccgcacggca gatttcggca aacagcagct
     1861 tgatatcgcg ctgctgtatc tgtgggtcgc gatcgaaatc cgaaatgaag gccgagtagg
     1921 gcagggcgga taacgccttg atatgccgga accccatctg gaaaacatgc tctgcgccgt
     1981 ggtgcagcag gaaggataga tgtgcatcgg cgtcctccgg gaggttcgcc tgactgatgg
     2041 ccagagagat gcatcctgtc gtcagatgga atgcctcgag caggccatct gccgtgcgct
     2101 cattggagaa tttcctggcg acgagcacgg tgatgccgct cagttcatca agcagtgtct
     2161 gggcggcctc ctgattgtcc gcgcgtcgtg ccagcgcttc gagtgccggc acgagatggg
     2221 gcgggcgcgt ggtgatgtgg gcgagttgca tggcgttaac ttgttgttcc tgacgacggt
     2281 gcatcacgca aagatgtcat cgcccagact tgggcgggcg ccttttttgg gcgtgccgag
     2341 cgctttgcct ggaacttgcc tgcgcaggcg cacgagtaac tcgtgggtgg ttctggccag
     2401 cgcatcgtca tgatggggct cttcttcatc gtcttcttcg gagcgtgcgc gcaactggtt
     2461 ctcgccgggt tgccagtcgg ggaagatctc gaacagtacc cgatcccagg cgtctgcatc
     2521 gctgcgggcg atttcgagcg cgagcgggat cacgtcgtgg tcggaagtcg tcaggccgct
     2581 gctgtgcggg ccgcgcgagt agatgtcgtt agcgagctcg atgatatcct cgatggctgt
     2641 cgagaaaacc acggcaaagg ctgtggttcc ccagtcggtc gacagcgcct catggatcag
     2701 cgccgcgcgc cgctccgggt cgtcgctgga aaagatcacg ccatgaagat gggcgaacag
     2761 ggattcggtc agcacctccg gctcgtcctc gatcatgtct tcgctccagg tggcggcgaa
     2821 gaaggccagg ctctcggccc aggctttggg cgagatcagc agcgtggcca gcgacagctt
     2881 gccgccatcg aaggccgcga ggtgcatcgc ggcgacttgc ggttccagcg tgtccagtat
     2941 cgtgaccacg gccaggtcgc cgaattgctc cgccgtctca ttgatggctt cttcagcgtg
     3001 ctcggtagag ccggccagaa ccagctcggt cacctgccgg ctgatcgccg ggaaattttt
     3061 gtcatcagcc attgccgtcc tcgcgcttgt cgaacagatc gatcacgtct tgcccgggga
     3121 cgcctcgttc atcatcctct atgtcgtcat cccagttctt ttcgttgtct tcttcttcca
     3181 gcgcttcgag cgactcgtct gcttcgctcc agcgcttggt gttcttgaac aggaatacgg
     3241 tcaggatcgc cttgcgtgcc agctccggat tcttggcgga aaccgcagcc agtgccttgg
     3301 ccgcctcgct gtccgcacaa gacaccatgg cgactgtctt tgcggcatcg ccttcatcgt
     3361 attgtttgca ttgcgccagc aacccggcag ggtcgtcgaa gcgcaggtgc tcggccagtg
     3421 caaagctcag catgttgaca tcgtcgatct ccgttccctt cgcgtattcg ccgatcaggg
     3481 aggcgatgac gcgcgcatag tttttacggt ccggcggatc gcccagggta tcttcgatct
     3541 ggacgcccag ttcgcgtgac tgataggcaa attcgtgatg tatgtttttg ttcatgagct
     3601 ggtgtactta agttgattga tgatggttct gcggtaccac aaggccgcag cgatcgaaca
     3661 atgagagcca cagggtgcgc gcctcgttat cgggatcgat gatgatgcga ttgttcaggc
     3721 tctggttgta ttcggccctt ttctggggat ccgaaagcaa atcataggcc gtcttgatcg
     3781 cattgaatcg aacctccgag ctggcgtgag aatttctgtc cgggtggtgg cgcatcgcaa
     3841 gcgagcgata ggccttcttg atctcttcag tcgtcgcggt cggtgaaacg ccgaggatgt
     3901 tgtatggatt tgccaacggg tgcctcctgt gagggggcga ttttacctat taacggcgag
     3961 attggtgggg ggattgctgc aattggcgga ttcgacggac gccgggccct gatttggcgt
     4021 cctgattgcg cgtcggggga aggccgcgcg cagcggtgaa tgcttcctgt gaggaggagc
     4081 ggctagctgg tagaatcgcg gcattgtcta tccaggagtg agcaatgagt tttaccgaat
     4141 ccgacgtgca gagcgcgttg aagagcctca tcgatccgaa taccaggaag gacttcgtca
     4201 gcggcaagtc cgtcaagaac gtcaaagtca gcggcaacga tgtgtcgctg gatatcacgc
     4261 tgggctaccc ggccaagagc gtctgggcgg aaattcaaac catggtcgag acgcacctca
     4321 aggcctccct tccgggcgcc ggcaagatca gcgcgaacgt gtcgagcaag gtggtgccgc
     4381 atgcggtaca gcgcggcgtg aagctggtgg agggggtgaa gaacatcatc gccgtggcga
     4441 gcggcaaggg cggcgtgggc aagtccacga cggcggtgaa tctggcgctg gcgctggccg
     4501 ccgagggcgc gcgcgtgggc atgctggacg ccgacatcta cggcccgtcg cagccgacca
     4561 tgctgggcat cgaaggtcgt ccgaagtcgc gcgatggcaa gtccatcgaa ccgatggaag
     4621 ggcacggcct gcaggcgatg tctatcggct tcatgatcga gggaaccgat gcgcctatga
     4681 tctggcgcgg cccgatggtg acgcaggcgc tcgatcagtt gctgcaccag acccgctggg
     4741 acaacctcga ctatctggtg gtggacttgc cgccgggcac cggtgacgtg caactgacgc
     4801 tggcgcagaa ggtgccggtg accggcgctg tgatcgtcac cacgccgcag gacatcgcgc
     4861 tgctggatgc gcgcaagggg ctcaagatgt tcgagaaggt cggcatcaag atcgtcggca
     4921 tcgtcgagaa catgagcacc catatctgtt cgcaatgcgg tcatgaagag catatcttcg
     4981 gcgcgggtgg cggcgagaag atgtgcgccg actacaacac cgaattcctc ggcagtctgc
     5041 cgctggacat ccgcatccgc gaacaggccg attcaggggc gccgacggtg gtgtccgacc
     5101 cggatggcaa tatcgccagg gtataccgcc agatcgcgcg ccgcgttgcg gtgaaggtcg
     5161 cggagatggc gcaggatcac agtgcggcat tcccgaagat tgtggtacaa aacacctgat
     5221 aacagattga aaacggggaa ctgaaatgac aatcggcggc ggcaagtcgt ccctggcggg
     5281 actggtaata caggggtcgg gcgtggcttc atcggggcgc attgcctcca tgctggaaaa
     5341 tgcccacatg ttcaaggata tggagtgggc tcagatcgaa gcgctgtcca actacatcca
     5401 gctctatcac gccgatccca acaccgtgct gtttcacgag ggcgatcgcg gcgacttcat
     5461 gtgcatcgtg ctggaaggca agcttgaagt ccgcaaggaa gatgcgcagc gtgtcgacaa
     5521 gaccgtcagc acggtgatgc cgggacgcag tctgggcgag atgaccatgg tcgacggcga
     5581 gccgcgctcg gcgacggcgg tggcggtgga agaatcgctg ttggcggtgc tcacgcagga
     5641 aaatttcaaa ctgatcatgc atgaccgtcc tgcgctggcg gtcaagctgc tgtacaagat
     5701 cgcgcagttg atcagccagc gcctgcgcca gaccagcggc atgctggtcg actacctcga
     5761 aaactagaac gcggcgcgcc cgccgctcac gcgttctatc ccgctcagat catgcgccga
     5821 gaatacctcg gcgtatcctg cctgtcgcaa caactcccgc actcgttccg cctgatcgta
     5881 gccatgctcg aacagcagcc agccatcggc aagcagatgc gccttcgcgt ccgcgatgat
     5941 gcgccggata tcgtccagtc cgtcggagcc tgaggccagc gccgtgcgcg gttcgaaacg
     6001 cagatcgccc tgctccagat gcgcatcggc ggaggcgatg taaggcgggt tcgagacgat
     6061 caggacgaag cgcgcgtcct gtagcgcgga gaaccagtcg ctgtgcaaca ggcgcacatt
     6121 ggcgagcttc aaccgccgcg cgttgtcctg tgccacctcc agcgcttcgg gggatgcatc
     6181 caccgcgatg acttcggcgt cggggcgcgc atgcgcgatg gacagtgcga tcgcgccgct
     6241 gccggtgccg aggtcgagca cgcgaccttg ctgcgggatt ttttgcaacg ccaactccac
     6301 cagcagctcg gtgtcggggc gcgggatcag cgtcgcgggg gtgaccttga aattcagccc
     6361 gaagaactct cgctcg
//
LOCUS       DASUIV010000221        16550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1076990, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000221 DASUIV010000000
VERSION     DASUIV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 16550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16550
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076990"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1395)
                     /locus_tag="VFW59_08020"
     CDS             complement(73..1395)
                     /locus_tag="VFW59_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5364203.1"
                     /translation="MSTASLFDAHQPAAPLAERLRPTHIDEVIGQTHLLGPGKPLRLA
                     FQSGKLHSMILWGPPGVGKTTLARLMASAFDADFVPLSAVLSGVKDIREAIAQAESTL
                     QQSGRHTILFVDEVHRFNKSQQDAFLPFVEQGLVTFIGATTENPSFELNNALLSRAQV
                     YVLQSLSEAEMVQLFERAQQTALQGITFDAAARERIIGYADGDARRLLNVLEQLQTAA
                     AAADLNNIDIAFLDNTLAQKLRRFDKGGEAFYDQISALHKSVRGSNPDAALYWFTRML
                     DGGADPRYLARRIVRMAWEDIGLADPRALQLTNDAAATYERLGSPEGELALAQAVLYL
                     AVAAKSNAGYVAYNAARAFVAQDGSREVPLHLRNAPTKLMKQLDYGRDYRYAHNEEGG
                     YAAGENYFPDGMPQVSFYEPTARGLEAKISEKLHHLRELDAAAKKRNG"
     gene            complement(1406..2020)
                     /gene="lolA"
                     /locus_tag="VFW59_08025"
     CDS             complement(1406..2020)
                     /gene="lolA"
                     /locus_tag="VFW59_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX5364204.1"
                     /translation="MKLIIAVILLCLAPLTVNAGGIDKLKEFIASTRSAQANFSQVVL
                     DQNGKRIQSASGVMQFVRPGKFRWTYQKPYEQLIVGDGAKFWLYDKDLNQVTVRKLDA
                     ALGSSPAALLSGSNEIERGFVLKESGVQDGLDWLQASPKSQDSSFNAIRMAFDAQSDL
                     VVMELNDQFGHKTVLRFSGLKRNPQLAPSLFKFVPPKGADVLAE"
     gene            complement(2094..4364)
                     /locus_tag="VFW59_08030"
     CDS             complement(2094..4364)
                     /locus_tag="VFW59_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5364205.1"
                     /translation="MAEAGGELPGNKLFMLLRESRWLLLAALAVYFTVGLYGYDPADT
                     AWSHSASHARTVNPGGVLGAYLSDLLFYLFGVSAWWWVVFILQRVWAGYRELRADSLF
                     CKRTFWVSVIGFAVLLLASSALEAIRLYTLQVALPLAPGGMFGALIGEALSHLLGFTG
                     ATLLLLALIATGLSVFSGLSWLRLVDWLGAALEGIWKWAWRTWHTRQDKRIGAQAMQE
                     RTVVVEEEKKRVVEHQPIHIEMPLAEVVKSTRVIEEKQAPLFSEMPDSPLPPLHLLDQ
                     PAPHVETVSVDTLEFTSRLIERKLKEFGVEVKVVGAYPGPVITRYEVEPAVGVKGSQI
                     VNLIKDLARALSVVSIRVVETIPGKAYMALELPNPKRQMVQLSEILGSQVYADMGSAL
                     TMAMGKDIAGKAVVADLAKMPHVLVAGTTGSGKSVAINAMILSLLYKSTPQQVRLLLV
                     DPKMLELSVYEGIPHLLCPVVTDMRQAASGLNWCVQEMDRRYKLMSALGVRNIAGYNQ
                     KVRDAIKAEQPLTNPFSITPDSPEPLEELPLIVVFIDELADLMMVVGKKIEELIARLA
                     QKARASGIHLVLATQRPSVDVITGLIKANVPTRVAFQVSSKIDSRTILDQMGAEALLG
                     QGDMLYLPPGSGYPQRVHGAFVSDQEVHRVTEYLKAQGMPQYIDGVLNSLEEAESEGG
                     EGGPALDAEADPLYDQAVDIVLKSRRASISSVQRQLRIGYNRAARLIEQMEASGIVSA
                     MQSNGNREVIAPARQD"
     gene            complement(4357..5022)
                     /locus_tag="VFW59_08035"
     CDS             complement(4357..5022)
                     /locus_tag="VFW59_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5364206.1"
                     /translation="MRILLVDDHVLFREGMRLVLQQLPDAIEVLEAGSFDDGLKLAEE
                     HPELDLALLDLNMPGSEGVVSIRYFNQRYPHIPVVVVSGEDSRMIMEKAMAAGALGFV
                     CKNSSASVMLSALNLVLSGGVYIPPQMLRQPVVVQRQETERADRRSSRTNEFGLTGRQ
                     MEVLQCIAAGLSNKEIADKLCLAEGTVKIHVAATYQTLRVSNRMEAVRVGEQLGLIGV
                     GNG"
     gene            complement(5040..6443)
                     /locus_tag="VFW59_08040"
     CDS             complement(5040..6443)
                     /locus_tag="VFW59_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5364207.1"
                     /translation="MSLLDKFYVWTSSDLNAENLPIRAGQLRARIDNYPQMIVSQLLV
                     GALLCAMLWDAVAHRVLIGWLCAQWVLHGVEAYHWWRYRGRLQGLDECRAWRTKLIVF
                     VTIIGSIWGAAVVLMFVPGDLAYQALLICVVLGVAAGAVTLNPVFPPSLYIFLGLLIL
                     PAVLVNLVAGDRTHFILGVLLSIYLAFVLNAGRELSRTFEQSLRGEWENTRLVGQLVK
                     EKARAEQALYSAESASQMKSKFFAAANHDLRQPMHALTMYLEVLKNAPLSASAKSSVE
                     QIERSVEVMSSMFDILLDISKLDAGVVQAQPELFALAPLLDKLRDEFVMLARDKGLRL
                     EVADCSEAVYSDPMLLERILRNLLSNAVRYTELGQVVLSCRETERGLQIEVCDSGIGI
                     APEHLPHIFEEYYQVNNKQRDRSKGLGLGLAIVERLARLLGGDLQVRSTLGEGTCFSL
                     VLPAPGADVMIPGDLAA"
     gene            complement(6440..7039)
                     /locus_tag="VFW59_08045"
     CDS             complement(6440..7039)
                     /locus_tag="VFW59_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5364208.1"
                     /translation="MFRLKLLIALLLILGLTACGEPKLPSPFHASEVGGKLAQADFRL
                     TDYNGKPRTLADFRGKAVVLFFGYIHCPDVCPTTLADLAQTMRLLGKDAERVQVLFVT
                     VDPERDTPALLAQYVPTFDPAFLALSGDAQATAQAAEAFAVSYRKQTTSSGYSMDHSS
                     SSFIIDPAGRVRLLAPYGQRAEWLADDLRLLLALDRSKR"
     gene            complement(7026..7736)
                     /locus_tag="VFW59_08050"
     CDS             complement(7026..7736)
                     /locus_tag="VFW59_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5364209.1"
                     /translation="MTEFGIVAVFFVGLLGGVHCLGMCGSIVGILTAQLPKEGARWPF
                     HLAYNGGRLASYAVAGALVGGIGQAGLLLRDEVPVQHLLFALSSLMLIVLGLYLAGIW
                     GFVRRIERMGGVLWQRIQPLTRRLLPVNTPLRALLLGSLWGWLPCGLVYSVLLTALAG
                     GSVQNGAAIMLAFGLGTLPNLLAIGLFWDRVRHWVQAPRVRLAAGLMVAGFGVYGLVK
                     VAYTLFTYGWSGSCHVPA"
     gene            complement(7729..8406)
                     /gene="bioD"
                     /locus_tag="VFW59_08055"
     CDS             complement(7729..8406)
                     /gene="bioD"
                     /locus_tag="VFW59_08055"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX5364210.1"
                     /translation="MNYFVTGTDTCVGKTLVSCALLHAFAARGNSVVGMKPVAAGCDD
                     ELHEDVRQLRAASNVQASRGQINPYSFVHPVAPHLAARFIGVRINIERILDSYAELNA
                     QSDVVIVEGAGGFCVPLNDEQDSADLVQRMNIPVVLVVGMRLGCLNHALLTVEAIAAR
                     GLTLAGWVANVVDADMAMLDENIATLKQRIAAPLLGIVPHRAAPDAREVASLLNLDLL
                     DQGDLHD"
     gene            complement(8403..9275)
                     /gene="bioC"
                     /locus_tag="VFW59_08060"
     CDS             complement(8403..9275)
                     /gene="bioC"
                     /locus_tag="VFW59_08060"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEX5364211.1"
                     /translation="MNEFEIDKKQVRRAFSRAAADYDAAAVMQREVCARMLERLDYIK
                     LQPSRILDIGSGTGWGTRQLAERYPSAQLVALDIAIGMLRNARGRSGWWQKLFGGAKQ
                     GYVCADVEALPVAAASVDMVWSNLALQWCNDLPATFVELHRVLKVDGLLMFSTFGPDT
                     LKELRRAFDGVDGHNHLNRFADMHDIGDMLVRGGFAEPVMDMEYLTLTYEDVRGVLHD
                     LKAIGAHNATSGRGQGLMGKQAWARLIDNYESSRSNGKLPATYEVVYGHAWKPKPRTT
                     RDGASIVQTSFKLK"
     gene            complement(9272..10066)
                     /gene="bioH"
                     /locus_tag="VFW59_08065"
     CDS             complement(9272..10066)
                     /gene="bioH"
                     /locus_tag="VFW59_08065"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEX5364212.1"
                     /translation="MSLHVETQGSGAPLLLIHGWGMHGGMWGEVAGRLAQDCRVSTVD
                     LPGHGRSGIEDRGSRVEDSALDDIVDALSAQFDEPLTVCGWSLGGQVALRWAQRHAPQ
                     VQRLVLVASTPCFVRREGWDCAMAADTLAAFAEALQQNYAQTLRRFLALQLRGSERER
                     ELLAELRAALFSRGEPELGALRSGLDILRDCDLRQALSGIGQPTLVIAGERDTLTPPQ
                     ASQYMVDSMPQAQLATITGAAHAPFLSHPDEFVACLQHFLHDRVFP"
     gene            complement(10050..11204)
                     /gene="bioF"
                     /locus_tag="VFW59_08070"
     CDS             complement(10050..11204)
                     /gene="bioF"
                     /locus_tag="VFW59_08070"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX5364213.1"
                     /translation="MPFTQLQNELDERAAQGLLRRRRTLDSPQSPHVSVDGKSYLAFS
                     SNDYLGLAHHPLLIAALQQGAQQWGVGAGAAHLVSGHFDPHQQLELQLAAFVGKPAAL
                     LFSTGYMANLGVVQALVGKGDTVFADKLNHASLNDAMLLSRAEVKRYRHNDMEQLAQL
                     LGQERAGRKIVITDAVFSMDGDMAPLSELLALCERYDAWLYVDDAHGFGVLGGQGRGS
                     LAHCGIASPRIIYMATLGKAAGVSGAFVAAEQVVIDTLINHARSYVYTTATPPALSVA
                     LSDSLKLIAQGDALRAHLTRLIVRLRAGLGDLPWQLMPSDTAIQPLLVGDNQQALALS
                     AGLRERGIWVAAIRPPTVPQGTARLRITLSAAHSEADVERLIGGLHELAC"
     gene            complement(11316..12311)
                     /gene="bioB"
                     /locus_tag="VFW59_08075"
     CDS             complement(11316..12311)
                     /gene="bioB"
                     /locus_tag="VFW59_08075"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX5364214.1"
                     /translation="MTTQTIAFVRPIKQSTAPQRWSVEEVEALFKLPFSDLMYRAQQI
                     HREHFDPNAVQLSTLLSIKTGGCSEDCGYCPQSAFHSAGVENKKMLDVAEVVNAAKAA
                     QQAGADRFCMGAAWREPYKEDMESVVEMVKSVRALGMETCATLGMLSDEQTEQLRVAG
                     LDYYNHNLDTSPEFYGDIISTRDYQDRLDTLDRVRRAGMHVCSGGIVGMGESLTQRAG
                     LVAQLANMDPYPESVPINNLVKVEGTPLAESAELDPLDFVRTIAVARITMPTARVRLS
                     AGRQQMSDAIQALCFLAGANSIFYGDQLLTTGNPDVARDRALLDKLGMYPFAEKH"
     gene            12438..13151
                     /locus_tag="VFW59_08080"
     CDS             12438..13151
                     /locus_tag="VFW59_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX5364215.1"
                     /translation="MSIISDAYLNICAMIGRALPAQPCVLCGIMSRNGLWCTACNRTL
                     PYLDGPHCPICALPTPHSERCGHCLSHPPEFNRTAAAFAYAYPLDQLIQAMKYREQLA
                     LAHAFATRLTPRIDRNHLPDYIIPMPLHPEKLRARGFNQSLLLAAAIARELNLALLPH
                     ACQRVRDTPPQSALPWKDRKKNVRDAFRCDLDLSGKHIALVDDVLTTGASLNALTAAI
                     KARGATEVSAWVVARTIRK"
     gene            complement(13199..14266)
                     /locus_tag="VFW59_08085"
     CDS             complement(13199..14266)
                     /locus_tag="VFW59_08085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5364216.1"
                     /translation="MSVINQVLDQLERRGAHAALKQNMVRVVPQARRNTLSWVLMASG
                     VLWLLGLGLWLGWTQGGANGGARAHRPPSVAMPIVFAPVSSVAVLTEVNSQPTVAPTQ
                     ERVQPASRLSLELSALPVQEPKRKPNPAASAAPVEATEKRSGKSQDAVAPPVYGADAN
                     LSLKQVSRAQQADAEFRRGVASMQQGRGAEAVASYEAALRLDATHDAARQALVALLLE
                     QHRRADAERVLQERLGSKPEHTGFAMLLARLQVERAAEDEALATLERSLRFAERNAEY
                     HAFVAALLQRNNRHAEAVAHYQTALQLQPNHGAWLMGYGLSLQALGRNDAAKAAFLHA
                     LESKTLSPELQAFVRQRLKGL"
     gene            complement(14263..15219)
                     /locus_tag="VFW59_08090"
     CDS             complement(14263..15219)
                     /locus_tag="VFW59_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5364217.1"
                     /translation="MYLQHFGLRELPFNLTPDTSFFFTCTSYQEALNTLLVAARNGEG
                     FIKITGEVGNGKTLLCRKFLAALNQDRRPTTAIGTQDHAEPVTSNSRFVTAYIPNPYL
                     EPRSLLLALAEEFRVPLEKDADQHQLLKGLTHALLECARKGQRALVCLDEAQAMPLES
                     LEVLRLLTNLETEKRKLLQVVLFGQSELNERLQHHSVRQLRQRISFQYELCGLARDEM
                     ERYLRHRLSVAKYAGETLFGKGAVATLYRVTHGTPRLVNILAHKALMLAYGEGRQHVL
                     ARHVRDAAADTPEARRDWLPWALAVTGAVLIIALGILWMRLR"
     gene            complement(15221..>16550)
                     /locus_tag="VFW59_08095"
     CDS             complement(15221..>16550)
                     /locus_tag="VFW59_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX5364218.1"
                     /translation="TVFDALEAIRQMYGYDYKVDGSRIYIQSLAMQTRIFQVNYLTGL
                     RQGTSSLRVTSGSVSDNPTGTTGAAGTTSSTTTRGLDSSKVSMTSSADFWVELGQSLQ
                     AIVGNVDGRSVVISPMSGVIVVRAMPDELKNVAAYLKASQVSIERQVILEAKIVEVQL
                     NDSFQSGVNWGAFKIGPNSATSLGQVSTGTALATTGALTNGTLTATPGLNMAAAAAAV
                     SGQAAGSLFGLAFQTSNFAALLNFLESQGNVHVLSSPRIATLNNQKAVLKVGTDEFFV
                     TNVTNTTTTGTATTSTPSVTLQPFFSGIALDVTPRIDENNEIILHVHPSVSLVTTVNK
                     TVNVGGIGGTLNLPLASSSISETDSIVRARDGQIVAIGGLMRQATFDDQSGLPGLPKA
                     VFGQTNKRSEKRELVILLKPTVVDDDKDWSQDITRSRDRFSSMSGQGGRP"
BASE COUNT         3363 a         5536 c         4683 g         2968 t
ORIGIN      
        1 cggttgtggg tattgggcgc cgcagcatgc tcgggccact gatcggaatt gctctggtaa
       61 tcgttcacgt tgctatccat ttcgtttttt tgctgcggca tccagctcgc gcaggtgatg
      121 caatttttcg gagatcttcg cttccagccc gcgcgcggtc ggctcgtaga aagaaacttg
      181 cggcatacca tccgggaaat aattctcgcc cgccgcataa ccgccctctt cgttgtgcgc
      241 gtagcggtag tcacgcccgt agtccagctg cttcatcagt ttggtcggcg cgttgcgcag
      301 atgcagcggc acttcgcgcg atccgtcctg agccacaaag gcgcgggcgg cattatacgc
      361 gacgtatccg gcattcgact tggccgcgac cgcgagatac agcaccgcct gtgccagcgc
      421 caattcgcct tccggcgagc ccaagcgttc gtaggtggcg gcggcatcgt tggtcaactg
      481 gagcgcgcgc ggatcggcca gcccgatgtc ctcccaggcc atgcgcacga tgcggcgcgc
      541 gaggtaacgc ggatcggcac cgccgtccag catgcgcgtg aaccagtaga gcgccgcatc
      601 cgggttggag ccgcgcaccg atttgtgcag cgccgaaatc tggtcgtaga acgcctcccc
      661 gcctttgtcg aaacggcgca gcttctgcgc cagcgtgttg tccagaaagg cgatatcgat
      721 gttgttcaga tcggctgctg cggccgcagt ctgcaactgc tccagcacgt tgagcagccg
      781 ccgcgcatcg ccgtccgcat agccgatgat gcgctcgcgt gccgccgcat cgaaggtgat
      841 gccctgcaat gccgtctgct gtgcgcgctc gaacagctgc accatctcgg cctcggacaa
      901 ggattgcaag acatagacct gcgcccgcga cagcagcgcg ttgttcagtt cgaacgacgg
      961 gttctcggtg gtcgcgccga tgaaggtgac cagtccctgc tcgacgaacg gcaggaaagc
     1021 gtcctgctgc gacttgttga aacggtgcac ctcatcgaca aacagtatcg tgtggcggcc
     1081 gctctgttgc agcgtcgact cggcctgcgc gatggcctcg cggatgtcct tcactccgga
     1141 caacaccgcc gacaagggca cgaagtcggc atcgaaggcc gaagccatca gccgcgccag
     1201 cgtggtcttg cccacacccg gcggacccca cagaatcatc gaatgcagct tgcccgactg
     1261 gaaggcgagt cgcagtggct tgccggggcc gagcagatgg gtctggccga tcacctcgtc
     1321 gatatgcgtg ggacgcaggc gttcggccag cggcgcggcg ggttgatgag catcgaacag
     1381 actggcggta gacattaaat cgagcttatt cggcgagcac atccgcgccc ttgggcggca
     1441 cgaacttgaa cagggatggt gcgagctgcg gattgcgctt caggccggag aaacgcagca
     1501 ccgtcttgtg gccgaactgg tcgttcagtt ccatgacgac cagatcggac tgcgcatcga
     1561 aggccatccg gatggcgttg aaactgctgt cctgcgactt cgggctagct tgcagccagt
     1621 ccagcccgtc ctgaacgccg ctctccttga gcacaaagcc gcgctcgatc tcgttgctgc
     1681 cggacagcaa ggcggccggg ctgctcccca gcgccgcatc gagcttgcgc accgtcacct
     1741 gattcaggtc cttgtcgtac agccagaatt tcgcaccatc tccgacgatg agttgttcat
     1801 agggtttctg ataggtccag cgaaacttgc cgggacgcac gaactgcatc acaccggatg
     1861 cgctctggat gcgcttgccg ttttgatcca gcaccacctg gctgaaattg gcctgcgcgg
     1921 aacgggtcga ggcgatgaat tccttgagtt tgtcgatgcc gccggcattc acggtcagcg
     1981 gcgcgagaca caacagaatt acagcaataa ttagcttcat ttattttctc tatataagga
     2041 ttggggattg aggatcgagc tgagatcggc ggatttccct cgatcctgaa tcctcaatcc
     2101 tgtcttgcgg gcgcgatcac ttcgcgattg ccgttgctct gcatcgcgct gacgatgccg
     2161 gacgcttcca tctgttcgat caggcgtgcg gcgcggttgt agccgatgcg caactggcgc
     2221 tgcaccgacg agatcgaggc gcggcgcgac ttgagcacga tgtccaccgc ctggtcatac
     2281 agcggatcgg cttcggcatc cagcgcgggg ccgccttcgc cgccctcgct ctctgcttcc
     2341 tccagcgaat tgagcacgcc atcgatgtac tgcggcatgc cctgcgcctt gaggtattcg
     2401 gtgacgcggt gcacctcctg atcggagacg aacgcgccgt gcacccgttg cgggtagccg
     2461 gaacccggcg gcaggtacag catgtcgccc tgccccagca aggcctccgc gcccatctga
     2521 tcgagaatgg tgcgcgagtc gatcttggag gatacctgga acgccacgcg cgtcggcacg
     2581 ttggccttga tcagaccggt gatcacgtcc accgacggac gctgggtcgc cagcaccaga
     2641 tggatgccgg aggcgcgcgc cttctgcgcc agacgcgcga tcagctcctc gatcttcttg
     2701 ccgaccacca tcatcaggtc ggccagctcg tcgatgaaca ccacgatcag cggcagctcc
     2761 tcgagcggtt ccgggctatc cggcgtgatc gagaacggat tggtcagcgg ctgctcggcc
     2821 ttgatcgcat cgcgcacctt ctggttataa ccggcgatgt tgcgcacgcc cagcgcggac
     2881 atcagcttgt agcgccggtc catctcctgc acgcaccagt tcagtcccga tgcggcctga
     2941 cgcatgtcgg tgaccaccgg acagagcaga tgcgggatac cttcgtatac cgacaattcc
     3001 agcatcttgg gatcgaccag cagcaagcgc acctgctgcg gcgtggattt gtacagcagc
     3061 gacaggatca tcgcgttgat cgccaccgac ttgcccgaac cggtggtgcc cgccaccagc
     3121 acatgcggca tcttggccag atcggccacc accgccttgc ccgcgatatc cttgcccatc
     3181 gccatggtca gcgccgagcc catgtcggca tacacctgcg aaccgaggat ttccgacagc
     3241 tgcaccatct gccgtttcgg attgggcagc tccagcgcca tataggcctt gccggggatg
     3301 gtctcgacca cgcggatgct gaccaccgac agcgcgcgcg ccagatcctt gatcagattg
     3361 acgatctggc tgcccttcac gccgaccgcc ggctcgacct cgtagcgcgt gatcaccggt
     3421 cccggatagg caccgaccac cttgacctcg acgccgaact ccttgagctt gcgctcgatc
     3481 aggcgcgagg tgaattccag cgtatcgacc gaaaccgttt cgacgtgggg tgcgggttga
     3541 tccagcagat gcaaaggcgg caacggcgag tccggcattt cggagaacag cggcgcctgt
     3601 ttctcttcga tcacccgcgt ggacttgacc acctccgcca gcggcatctc gatatggatg
     3661 ggctgatgct ccaccacgcg cttcttctct tcttcgacga cgaccgtgcg ctcctgcatc
     3721 gcctgcgcac cgatacgctt gtcctgtcgc gtatgccagg tacgccatgc ccatttccag
     3781 atgccctcca gcgccgcccc cagccagtcc accagtcgca gccaggacag gccgctgaac
     3841 acgctcagac ccgtggcgat cagcgccagc aacagcagcg tcgcgccggt aaagcccagc
     3901 agatgactga gcgcctcgcc gatcagggca ccgaacatcc cgccgggtgc cagcggcagc
     3961 gcgacctgca aggtatacag gcggatagcc tccagtgcgc tgctggccag caataacacc
     4021 gcgaacccga tcaccgaaac ccagaaagta cgcttgcaga agagactgtc ggcgcgcagt
     4081 tcgcgatagc ctgcccacac gcgctgcagg atgaacacca cccaccacca agcggaaacg
     4141 ccgaacagat agaacaacaa atcggaaaga tacgcgccca gcacaccgcc gggattaacg
     4201 gtccgcgcat ggctggcgct gtgcgaccag gccgtatcgg cgggatcata gccgtacagc
     4261 cccaccgtga aataaacggc cagtgcggcc agcagcagcc agcgcgattc gcgcaacagc
     4321 atgaacagtt tgttgcccgg cagttcgccg cctgcctcag ccattgccga caccgatcaa
     4381 acctaactgc tcgcccacgc gcaccgcctc catgcggttg ctcacgcgca aggtctggta
     4441 agtcgccgcc acgtggatct tgaccgtacc ctcggcaaga cacagcttgt cggcgatctc
     4501 cttattggac aaaccggccg cgatgcactg caacacctcc atctgacgcc cggtcaggcc
     4561 gaattcattg gtgcgcgagc tgcgccggtc ggcgcgttcc gtctcctgtc tttgcacaac
     4621 cacgggctgg cgcaacatct gaggcgggat ataaacgccc cccgacagca ccagattcag
     4681 cgcgctcagc atcaccgacg cgctggagtt cttgcacacg aaacccaacg cgcccgccgc
     4741 catcgctttc tccatgatca tccgagagtc ctcgccagac acgaccacca ccgggatatg
     4801 cggataacgc tgattgaaat accggattga aacgaccccc tcgctgccgg gcatattcaa
     4861 atccagcagc gccagatcca gctcaggatg ttcctcggcc aacttcaacc cgtcgtcaaa
     4921 actacccgcc tccagcacct cgatcgcatc gggcaactgc tgcaatacca gacgcatgcc
     4981 ctcgcggaac agcacatggt catccaccag caaaattctc atatatcccc ctgtcccggc
     5041 tatgctgcca agtcgcccgg tatcataaca tccgctccgg gtgctggcag caccaagcta
     5101 aagcaggttc cctcccccag cgtagaacgc acctgcaaat cgccgcccag caatctggcc
     5161 aagcgttcga cgatcgccag tcccagaccc agccccttgc tccggtcgcg ctgcttattg
     5221 ttcacctgat aatactcctc gaagatatgc ggcaaatgtt ccggagcaat gccgatgccg
     5281 ctgtcgcaca cctcgatttg caggcctcgt tctgtctccc tgcaactgag caccacctgc
     5341 cctagctcgg tgtaacgcac cgcgttcgaa agcagattgc gcaggatgcg ctccaacagc
     5401 atcggatcgc tatagacggc ttcggaacag tccgcgacct caagccgcaa gcccttgtcc
     5461 cgcgccagca tgacgaactc gtctcgcaac ttatccagca gcggcgccaa cgcgaacagc
     5521 tccggctgcg cctgcaccac ccccgcatcg agcttggaaa tatccagcag gatatcgaac
     5581 atcgaactca tgacctcaac cgaacgctct atctgctcga ccgacgactt cgccgacgca
     5641 ctcaacggag cgttcttcaa cacctccaga tacatggtca aggcatgcat cggctggcgc
     5701 aaatcatggt tggccgcagc gaagaacttc gatttcatct gactggccga ctcggcggaa
     5761 tatagcgcct gttcggcgcg cgccttctcc ttcaccagct gcccgaccag ccgggtattc
     5821 tcccattcgc cgcgcagcga ctgctcgaag gtgcggctca actcccgccc ggcgttcaac
     5881 acgaacgcca gatagatgga cagcaatacg cccagaatga agtgcgtccg gtcaccggcg
     5941 accagattga ccaagacagc aggaagaatc agcagtccca ggaagatata cagcgaggga
     6001 ggaaagaccg gattgagggt caccgcaccg gcagccaccc ccagcaccac gcaaatcagc
     6061 aacgcctgat aggccagatc gccaggaacg aacatcagca caacggcagc gccccatatc
     6121 gagccaatta tggtgacaaa cacgatcaat ttcgtgcgcc atgccctgca ttcatccagc
     6181 ccctgtaacc gcccccggta acgccaccag tgataggcct cgactccatg caagacccac
     6241 tgggcgcaca gccagccgat cagcacacgg tgcgccaccg catcccacaa catggcgcac
     6301 agcaacgcgc ccaccagcaa ctggctgacg atcatctgag gataattgtc gatacgcgcg
     6361 cgcaactgcc cggcgcgaat cggcagattt tcagcattga gatcgctgga agtccagacg
     6421 taaaacttat cgagcagact caccgtttgc tccgatccag cgccagcaac aggcgaagat
     6481 catccgccag ccactcggcg cgttgcccgt agggcgcgag caaacgcacc cggcctgcgg
     6541 ggtcgataat aaaagagctc gagctgtgat ccatgctgta gcccgagctg gtcgtctgct
     6601 tgcgatagga gacagcgaac gcctctgccg cctgcgctgt cgcctgagca tcaccgctca
     6661 gcgccaggaa agcaggatcg aaggtcggca catactgcgc cagcaacgcc ggcgtgtcgc
     6721 gctccggatc gacggtaacg aacaatacct gcacccgctc cgcgtcttta cccagcaggc
     6781 gcatcgtttg cgccagatcg gccagcgtag tgggacacac atccggacaa tggatgtaac
     6841 cgaagaacag caccaccgcc ttgccgcgaa aatccgccag cgtgcgcggc ttgccgttgt
     6901 agtcggtcaa acgaaaatcg gcctgcgcca acttgccccc gacctcgctg gcatgaaagg
     6961 gcgacggcaa tttgggctcg ccgcaagcgg tgagcccaag gatcagcaac aaggcgatca
     7021 gcagtttaag ccggaacatg acaacttcca ctccatccat aggtaaataa ggtgtacgcc
     7081 accttgacca gtccatacac gccaaacccg gccaccatca ggcccgcagc cagccgcacg
     7141 cgcggcgcct gcacccagtg cctgacgcga tcccagaaca gaccgatggc cagcaagttc
     7201 ggcaaggtgc ccaagccgaa tgccagcatg atggctgcgc cgttttgcac cgaaccgcca
     7261 gccagcgcgg tgagcagcac actgtacacc agaccgcagg gcaaccaccc ccacaaactg
     7321 ccgagcagca aggctcgcaa cggcgtattc accggcaaca ggcgccgggt cagcggctga
     7381 atgcgctgcc ataacacccc acccatccgt tcgatgcgcc gcacaaagcc ccagatgccg
     7441 gcgagataca agcccagcac gatcagcatc aggctggaca aggcaaacag cagatgctgc
     7501 accggcacct cgtcgcgcaa cagcaatccg gcctgaccga tgccgccgac cagcgctccc
     7561 gccacggcat aactcgccag ccgcccgcca ttgtaggcca gatggaacgg ccagcgcgcg
     7621 ccctccttgg ggagctgtgc cgtcaggatg ccgacgatgc tgccgcacat gccgaggcag
     7681 tgcacgccgc ccaacaggcc gacgaaaaat accgcgacga taccgaactc agtcatgcaa
     7741 atccccctga tccagcaaat ccaaattgag cagcgaggca acttcgcgcg catcgggcgc
     7801 cgcgcgatgc ggcacgatac ccagcaacgg cgcggcgata cgctgtttca gcgtggcgat
     7861 attctcgtcc agcatcgcca tgtcggcatc gaccacattc gccacccagc ccgccagcgt
     7921 caggccgcgc gctgcgatcg cctcgacggt gagcaaggca tgattcaaac accccagacg
     7981 catgcctacc accagcacca ccggaatatt cattcgttgc accaggtcgg cactgtcctg
     8041 ctcgtcgttc aagggcacgc aaaaaccacc ggcgccttcc acgatcacca catcggactg
     8101 agcgttcaac tccgcataac tgtcaagaat gcgctcgatg ttgatgcgca cgccgatgaa
     8161 gcgcgcagcc agatgcggcg cgaccggatg tacgaaacta taagggttga tctggccgcg
     8221 gctggcttgc acattgctgg ccgcacgcag ttgccgaacg tcttcgtgca gctcgtcgtc
     8281 gcatcctgcg gcaaccggct tcatcccgac caccgaattt ccgcgcgccg cgaaggcatg
     8341 caacagcgcg cagctgacca gcgtcttgcc gacacaggta tcggttcctg tgacgaaata
     8401 gttcatttca gcttgaatga ggtctgaacg atagatgcgc cgtcgcgcgt ggtgcgcggc
     8461 ttaggcttcc aagcatgacc gtaaaccact tcataggtag caggcaactt gccgttgctg
     8521 cgcgaacttt cgtagttgtc tatcagccgc gcccaggcct gtttgcccat caacccctgt
     8581 ccgcgcccgc tcgttgcatt gtgcgcgccg atggccttga gatcgtgcaa cacgccgcgc
     8641 acatcctcat aagtcagggt gagatattcc atatccatca ccggctcggc gaaaccgccg
     8701 cgcaccagca tgtcgccgat gtcgtgcatg tcggcaaagc gattcaggtg attgtgtccg
     8761 tccaccccat cgaatgcccg ccgcaattcc ttcagcgtat ccgggccgaa ggtgctgaac
     8821 atcagcaacc cgtccacctt gagcacgcga tgcaactcca cgaaggttgc gggcaaatca
     8881 ttgcaccact gcaaggccag attggaccac accatatcga cgctggctgc cgccactggc
     8941 aaggcctcga catcggcgca cacatagccc tgtttcgcgc cgccgaacaa tttctgccac
     9001 cagccagaac gtccgcgcgc attgcgcagc atgccgatgg cgatgtccag tgcaaccagc
     9061 tgcgccgacg gatagcgctc tgccaactga cgcgtccccc agcccgtgcc gctgccgata
     9121 tcgaggatgc gcgacggttg cagcttgatg taatcgagcc gctccagcat gcgtgcgcat
     9181 acctcgcgct gcatcactgc agccgcatcg taatctgccg ccgcacgact gaaggcgcgg
     9241 cgcacttgct tcttgtcgat ttcaaattcg ttcatggaaa aactcggtca tgcaaaaaat
     9301 gctgcaaaca tgcgacaaac tcatcggggt gagacaggaa aggcgcatga gctgcaccgg
     9361 ttatcgtcgc aagctgcgcc tgcggcatgc tgtccaccat atattgcgag gcctgcggtg
     9421 gagtgagggt atcacgttcc ccggcgatca ccagcgtggg ctgcccgatg ccggacagcg
     9481 cctgacgcag gtcgcagtca cgcaggatgt ccagcccgga ccgcaatgcg cccagctcgg
     9541 gctcaccacg actgaacagg gcggcacgca actcggcgag caactcgcgc tcacgctcac
     9601 tgccgcgcaa ttgcagcgcc agaaaacggc gcagcgtctg ggcatagttc tgttgcaatg
     9661 cctcggcgaa agccgccagc gtatccgccg ccatcgcaca atcccaaccc tccctgcgca
     9721 cgaagcacgg cgtactcgct accagaacca aacgctgcac ctgcggtgca tggcgctgtg
     9781 cccaacgcaa cgcgacctgc ccgcccagcg accagccgca gactgtcaac ggctcatcga
     9841 actgcgcaga gagcgcatcg acaatgtcat ccagcgcaga atcctcgacc ctcgatcctc
     9901 gatcctcgat ccccgatctt ccatgtcccg gcagatccac cgtgctgacg cggcagtcct
     9961 gcgccagtcg cccggctacc tccccccaca tgccgccgtg catgccccag ccgtggatca
    10021 gcagcagcgg tgcgccgctg ccttgcgtct caacatgcaa gctcatgcaa tcccccgatc
    10081 aatcgctcga cgtccgcctc gctgtgcgcg gcagacaggg tgatgcgcag ccgcgccgtc
    10141 ccctgcggca cagtgggcgg gcggatcgcc gcgacccaga tgccgcgctc gcgcaggcct
    10201 gcgctcaatg ccaaggcctg ctggttatcg ccgaccagca gcggctggat ggcggtgtcc
    10261 gacggcatca attgccaagg cagatcgccg agtcctgcgc gcaggcgaac gatcagccgg
    10321 gtcagatgcg cgcgcagcgc gtcaccttgc gcaatcagct tcaggctatc ggatagcgct
    10381 acagataaag caggcggggt ggccgtcgtg tagacgtagc tgcgcgcatg gttgatcagc
    10441 gtgtcgatca ccacttgctc cgccgcgacg aaggcgccgg acactcccgc cgccttgccc
    10501 agcgtcgcca tgtagatgat gcggggtgac gcgatgccgc aatgggccag cgagccgcgc
    10561 ccctgccctc ccagcacacc gaagccatgc gcatcatcca catacagcca ggcatcgtat
    10621 cgttcgcaca gagccaacaa ctcagacaac ggcgccatat cgccgtccat gctgaacacg
    10681 gcatcggtga taacgatttt tcgcccagcc ctctcctggc ccagcaactg cgccagttgc
    10741 tccatgtcgt tatgccggta gcgtttcacc tcggcacggg acagcagcat cgcatcgttc
    10801 agcgaggcgt gattgagctt gtcggcgaac accgtgtcgc ccttgccgac cagcgcctgg
    10861 accacgccga ggttcgccat gtagccggtg gagaaaagca acgcggccgg cttgccgacg
    10921 aaggcggcga gttgcagttc gagttgctga tgcggatcga aatgcccgct caccagatgc
    10981 gccgctcccg cgccgacccc ccattgctgc gcgccctgct gcagcgcggc aatcagcaga
    11041 ggatgatggg cgaggcccag atagtcgttg ctgctgaagg ccagatagga cttgccatcc
    11101 actgaaacat gcggcgactg gggactgtcc agcgtgcgtc gccgacgcaa caggccctgc
    11161 gctgcgcgtt cgtcgagttc gttttgaagt tgagtgaatg gcatcaatat cggtaggatg
    11221 ggttgagcac agcgataccc aacaacatac acactatcat caggatgatg ggtatcgctg
    11281 cgctcaaccc atcctacgtt ctgcggataa gttctttaat gtttttccgc gaacggatac
    11341 atgcccagct tatccagcaa tgcccggtca cgcgccacat ccgggttgcc tgtggtcagc
    11401 agctgatcgc cgtagaaaat ggaattcgca ccggcgagga aacacaacgc ctgtatcgca
    11461 tcggacatct gctggcgtcc tgcagacagg cgcacacgcg cagtcggcat ggtgatgcgc
    11521 gccacggcga tggtgcgcac gaaatccagc ggatcgagtt ctgcgctctc tgccagcggc
    11581 gtgccttcga ccttgaccag attgttgatc ggcacgctct cggggtacgg atccatgttg
    11641 gccagttgcg cgaccagccc ggcgcgctgc gtcaggctct cgcccatgcc gacgatgccg
    11701 ccgctgcata catgcatgcc cgcgcgacgc acccgatcca gcgtgtccag cctgtcctga
    11761 tagtcgcgcg tgctgatgat gtcgccgtaa aactccggcg aggtatcgag attgtgattg
    11821 tagtaatcga gcccggccac gcgcagttgc tcggtctgtt cgtcgctcag catgcccagc
    11881 gtggcgcagg tttccatgcc cagcgcgcgc accgacttga ccatctcgac cacggattcc
    11941 atatcctctt tgtaaggctc gcgccatgcc gcgcccatgc agaagcgatc cgcaccagcc
    12001 tgctgtgcgg ccttggcggc gttcaccacc tcggccacat ccagcatctt cttgttctcg
    12061 acacccgcag agtggaatgc cgactgcgga cagtagccgc agtcctccga acagccgccg
    12121 gtcttgatcg acagcaaggt ggacaactgc accgcattcg gatcgaaatg ttcgcgatga
    12181 atctgttgag cccgatacat cagatcagag aacggcagct tgaacagcgc ctcgacctcc
    12241 tccacactcc agcgttgcgg ggcggtactc tgtttgatcg gacggacgaa tgcgatggtc
    12301 tgggtagtca tgtcggctag tgatggatag ttttttaagc acgggattga acggcacgct
    12361 tcacaagtga aacgcttctc attccgcttc ggtatgatgc gacgagagcg gcgcatcatc
    12421 cttgaaagga caataacttg tcaattatca gcgatgccta tctgaacatc tgcgcaatga
    12481 ttgggcgagc attgcccgcc cagccctgcg tgctgtgcgg gatcatgagc cgcaacgggc
    12541 tgtggtgcac cgcctgcaac cggaccctgc cctatcttga cgggccccac tgcccgatat
    12601 gcgccctgcc gacgccacac agcgaacggt gcggccattg cctgagtcac ccgcccgagt
    12661 tcaatcgcac cgccgccgcc ttcgcctacg cctacccact ggaccagctg atccaggcga
    12721 tgaaataccg cgaacaactg gcgctagccc atgccttcgc cacccggctc acgccacgaa
    12781 tagaccgcaa ccatctgccc gattacatca ttcccatgcc gctgcatccg gaaaaactgc
    12841 gcgcacgagg cttcaaccaa tcgctgctgc ttgcggccgc catcgcccgc gaactgaatc
    12901 tcgcactgct gccccatgca tgccagcgcg tgcgcgacac tccgccacaa tcggcgctgc
    12961 cctggaagga tcgcaagaag aatgtacgcg atgcgtttcg ctgcgatctg gatctgagcg
    13021 gaaaacacat cgcgctggta gatgacgtac tgacgaccgg ggccagcctg aatgcgctga
    13081 ctgcggcgat aaaggcacgc ggggcgaccg aagtgtcggc ctgggtcgtc gcacggacga
    13141 tcaggaaata ggatacgggg gtgagttgaa ctcgcggact gccgtatcgc gaccgccgct
    13201 acaaaccctt gagtcgctgc cgaacgaagg cctgcaactc gggactgagc gtcttgctct
    13261 ccaacgcatg caggaacgcg gccttcgccg catcattgcg tcccagcgcc tgcaaggaaa
    13321 ggccatagcc catcaaccac gcgccgtgat tcggctgtag ctgcaaggcc gtctgatagt
    13381 gagcgactgc ctcggcgtga cggttgttac gttgcagcaa cgcagcgaca aaggcgtggt
    13441 attcggcgtt tctctccgcg aagcgcagcg atctttccag cgtggccagc gcctcatcct
    13501 ccgcagcacg ttccacctgc aggcgcgcca acaacatcgc gaaaccggtg tgctccggct
    13561 tgctccccaa acgctcttgc agcacccgtt cagcatcggc acgccgatgc tgttccagca
    13621 acagcgccac cagcgcctga cgcgccgcat catgggtggc atcgagacgc agcgccgcct
    13681 catagctcgc aaccgcctcg gcgcctcgcc cctgctgcat cgatgcgaca ccccggcgga
    13741 actcggcatc ggcctgttgc gcccgactga cctgcttcaa cgacaggttg gcatcggcgc
    13801 cataaacagg cggcgcgacc gcatcctgcg acttgcccga tcttttttcg gtcgcctcga
    13861 ccggcgctgc agaggcagcc ggattcggct tgcgcttcgg ctcctgcacg ggcagcgcgc
    13921 tcaattcgag gctaaggcgc gaggctggct gcacccgctc ctgtgtcggc gcgaccgtcg
    13981 gctggctatt cacctcggtc aagacggcga cgctggaaac aggcgcaaac acgataggca
    14041 tggcgacgct cggcgggcga tgggctcgcg ctcccccatt cgcccccccc tgcgtccacc
    14101 ctagccacag gcccaaccct agcagccata acacgcccga tgccatcagc acccagctga
    14161 gggtattgcg ccgggcttgt ggcacaacgc gcaccatgtt ttgcttgaga gcggcgtgcg
    14221 caccgcgccg ttcgagctga tccagaacct gattgatgac actcatcgaa gccgcatcca
    14281 caagatgccc agcgcaatga tgagcacggc ccccgtcaca gccagcgccc aaggcaacca
    14341 gtcacgccgg gcttccggcg tatcggcggc ggcatcgcgg acatgccggg ccagcacatg
    14401 ctggcggcct tcgccgtagg ccaacatcag ggctttgtgc gcaaggatat tgaccagcct
    14461 gggcgtgccg tgggtgacac gatacagcgt agctaccgcc cccttgccga acagcgtctc
    14521 accggcatat tttgcgaccg acaaacgatg acgcagatag cgctccatct cgtcgcgcgc
    14581 caaaccgcac agttcatact gaaagctgat gcgctggcgc agctggcgca cggaatgatg
    14641 ctgcaggcgt tcattcaact cggactgacc gaacagcacc acctgcaaca acttgcgctt
    14701 ctcggtttcc agattggtga gcagacgcag cacttccaga ctctccagcg gcatggcctg
    14761 cgcctcgtcc agacacacca gcgcgcgctg ccccttgcgt gcacactcca gcagcgcatg
    14821 ggtcaggccc ttcagcaact gatgctggtc ggcatccttt tccagcggca cgcggaattc
    14881 ttcagccagc gccagcaaca ggctgcgcgg ctcaagataa ggattgggga tataggccgt
    14941 gacgaatcgc gagttggagg tcacgggctc agcatgatcc tgagtgccga tggcagtcgt
    15001 ggggcgacga tcctgattca gcgccgcgag gaacttgcgg cacagcaagg tcttgccgtt
    15061 acccacctct ccggtgatct tgatgaaacc ctcgccatta cgcgccgcta ccagcagggt
    15121 attgagcgct tcctgataac tggtgcaggt aaagaagaag ctggtatccg gtgtcagatt
    15181 gaagggaagt tcgcgcaggc caaaatgctg tagatacatg tcaaggcctg ccgccctgcc
    15241 cgctcatact gctgaaacga tcgcggctgc gcgtgatgtc ctgcgaccag tccttgtcgt
    15301 catccaccac ggtcggcttc agcaggatca ccaattcccg cttctcgctg cgcttgttgg
    15361 tctgtccgaa cactgccttc ggcagccccg gcaaaccgga ttgatcgtcg aaggtggcct
    15421 ggcgcatcaa gccgccgatc gccacgatct gtccgtcgcg cgcacgcacg atgctatcgg
    15481 tctcggaaat ggaactggag gccaagggca gattcaacgt gccaccgatg ccgccgacat
    15541 tgaccgtctt gttgaccgtg gtcaccaggc tgacggaagg atggacgtgc agaatgatct
    15601 cgttgttctc gtcgatgcgc ggcgtcacat ccagtgcgat accggagaag aacggttgca
    15661 gcgtcacgct cggcgtggaa gtggtcgccg taccggtggt cgtggtattg gtgacgttgg
    15721 tcacgaagaa ttcgtccgtt cccactttaa gcacggcctt ctgattgttc agcgtcgcga
    15781 tacgcggact ggacagcaca tgtacgttgc cctgcgactc cagaaagttc aacagcgcag
    15841 caaagttgct ggtctggaat gccagcccga acaacgaccc ggcagcctgc cccgaaacgg
    15901 ccgctgcggc tgcggccatg ttgagaccgg gcgtggccgt cagcgtgcca ttggtcaatg
    15961 ccccggtggt ggcaagcgcg gtgccggtgg aaacttgacc gagcgacgtc gcgctgttcg
    16021 gaccgatctt gaaggccccc cagttcacgc cggactgaaa gctatcgttc aactgcactt
    16081 caacgatctt cgcctccagg atcacctgac gctcgatgga gacttgcgat gccttgaggt
    16141 aggccgccac atttttcaat tcgtccggca tcgcgcgcac cacgatgacg ccggacatcg
    16201 ggctgatcac cacactgcgc ccgtccacat tgccgacgat agcctgtaac gactgcccca
    16261 actcgaccca gaaatcggca ctgctggtca tgctcacctt gctgctgtcg agccctcgcg
    16321 tagtggtgct ggatgtggtt ccggcggctc cggtcgtgcc ggttggattg tccgagaccg
    16381 aaccggaagt cacgcgcaac gaggaagttc cctggcgcag tccagtcaga taattcacct
    16441 gaaagatccg cgtctgcatc gccaacgact gaatgtagat gcgcgagcca tccaccttgt
    16501 aatcgtagcc atacatctgg cggatcgctt ccagcgcatc gaataccgtc
//
LOCUS       DASUIV010000222         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_239919, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000222 DASUIV010000000
VERSION     DASUIV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239919"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..609)
                     /locus_tag="VFW59_08100"
     CDS             complement(61..609)
                     /locus_tag="VFW59_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364219.1"
                     /translation="MKKIALALSVLTLSACSTFTPQRYSISADTNVALKSIGVGNINV
                     GAFSGPSSFDKACRGAGPIAPPDNMSFEAYIQKALADELKVAGMFDDKTPKSTLTGAV
                     EKLHFSSSRGLTGGEWDISLRISSSNGKSLFVSEHYEFESGFIADTACKQTAEAYLPA
                     VQNLIGKAVKTPEFKSLLVQSN"
     gene            complement(815..1270)
                     /locus_tag="VFW59_08105"
     CDS             complement(815..1270)
                     /locus_tag="VFW59_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEX5364220.1"
                     /translation="MGKFIWTEQLNIGIDVIDHQHRRIVEYINQLDDARSNGQSREEI
                     AWLINDLVDYTISHFGFEESLQEEAKYPFIKAHKKVHDLFAQRVGEFQERFNKGEDVS
                     KALNSLLITWLFNHIKRDDADYVATVKEHLKNQSGVVEKKKGLFARLFK"
     gene            1690..2136
                     /gene="mraZ"
                     /locus_tag="VFW59_08110"
     CDS             1690..2136
                     /gene="mraZ"
                     /locus_tag="VFW59_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5364221.1"
                     /translation="MFQGSTQLSVDSKGRLAIPARYRDMLLAHCAGSLVLTADADGCL
                     LIYPQPDWQPIREKLLKLSALNPRIRALQRLLVGHAEEVVMDGAGRVLVSPTLRSYAA
                     LDKHVMLIGQGNKFELWDEAKWQAQQDIALSLMSGELPPELEGFSL"
     gene            2157..3080
                     /gene="rsmH"
                     /locus_tag="VFW59_08115"
     CDS             2157..3080
                     /gene="rsmH"
                     /locus_tag="VFW59_08115"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5364222.1"
                     /translation="MTHETVLLSEAVDALEVKPDGIYVDGTFGRGGHSARILAQLGPN
                     GRLIALDKDPTAVAVGRQWTDARFTMVHRGFIELPQVLDELQVTQVDGVLLDLGVSSP
                     QLDEAVRGFSFRFDAPLDMRMDTSSGMTAAQWLATVDEGLLAEVIRDYGEERFAKQVA
                     RAIVTARAVEPIETTRQLVELVGKTVRTREAGQNPATRTFQAIRIYLNRELEELAGVL
                     PECVTRLKPGGRLAVISFHSLEDRIVKHFMRDMAEGDKLPRNVPIRASEVPQGRMHLI
                     GKAVRATAEEVQANPRARSAVLRIAERNDVA"
     gene            3070..3372
                     /gene="ftsL"
                     /locus_tag="VFW59_08120"
     CDS             3070..3372
                     /gene="ftsL"
                     /locus_tag="VFW59_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX5364223.1"
                     /translation="MSRSGNGLNVLLLVAVVMCALSVVTSQHKARKLYIELQKEKESA
                     QQMDVEWGQLQLELSTWATPARVENIAARQLRMQLPANGQVQFIRVEPNGQRDNQS"
     gene            3369..>3640
                     /locus_tag="VFW59_08125"
     CDS             3369..>3640
                     /locus_tag="VFW59_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5364224.1"
                     /translation="MKYAASAKKDVPAKLPGWRATALFVALLAGLLGLIGRSAYLQVM
                     HEDFLQEKGNARYSRVIEVSAHRGMISDRHGEPLAVSTPVESVWASP"
BASE COUNT          734 a          902 c         1175 g          829 t
ORIGIN      
        1 gaagcccttg tagttaacta gacactaaaa atgtccccta ttcttcttcg ggtgggccga
       61 ctagttggat tgaacgagta gcgatttgaa ttcaggggtt ttgactgcct tgcctatcag
      121 attttggact gcagggaggt aagcctccgc agtctgcttg catgcggtat ctgcaataaa
      181 gccgctttca aattcatagt gttccgaaac gaagagtgac ttgccgtttg acgaactgat
      241 gcgcaagcta atatcccact cgccgccagt gaggccgcgc gatgaagaga aatgcagttt
      301 ttctacggct ccggtaagtg ttgatttggg cgtcttgtcg tcaaacattc ctgcaacttt
      361 caattcatcc gcaagcgctt tctggatata cgcctcgaag ctcatgttgt ccggaggcgc
      421 gattggcccc gcgcctctgc aggctttatc aaacgatgat gggccggaga atgctccaac
      481 gttgatgttg ccaacgccaa tcgactttaa ggccacattg gtgtcggcag aaatgctgta
      541 acgctgtggt gtgaacgttg aacacgcgct gagtgtaagg acggaaaggg caagtgcgat
      601 tttcttcata ataaaactcc ctgagtgaaa ataaatccga caaaaagtgg gagcggcgta
      661 cttgtgcgct gccccctgta tttttaacga ctgtattcgc tttgcattat ccaaacaacc
      721 tagcaaggta ggtaggttac gcgccgcaaa aaatctgcta ggaggttcaa cggatagtct
      781 ggatgcggga tgtccgcgct gcgctcgggg ctggttactt gaacagccgg gcgaacagcc
      841 ctttcttctt ttccacgacg ccgctctggt ttttcaggtg ctcttttacc gttgccacat
      901 agtccgcatc gtcgcgcttg atgtgattga acagccaggt gatcagcagg ctgttcaggg
      961 ccttggagac gtcttcgcct ttgttgaagc gctcctggaa ttcgcctacc cgttgagcga
     1021 acaggtcgtg gaccttcttg tgcgccttga tgaacgggta cttggcttct tcctgcaggc
     1081 tttcttcgaa gccgaagtgg gagatggtgt agtcgaccag gtcgttgatc agccaggcga
     1141 tctcttcgcg cgattgtccg ttggagcgcg cgtcgtcgag ttgattgatg tattccacga
     1201 tcctgcggtg ctggtggtcg atgacgtcga tgccgatgtt cagttgctct gtccagatga
     1261 acttgcccac ggtgatctcc tctagcttgg ttacacatgc tcccggtgcg cggcttgcgg
     1321 cgtcgaggcg ttcagattgt ttcccgggtt gcgtgccgtc aaggtacaag tgccatcctc
     1381 gcaggtcatg ccggctgcca atctgccggg ggttgatcgc ccagttaaag caatttctta
     1441 acaatcgcgc gcaccttatc ccatgtcaaa agcaggggca agctgcctga atgggtaggt
     1501 ctcgcatcac aatcgacttt agcttgtttc tgcaattcat tgctttttaa gcatatttgt
     1561 tttgcattaa ctctgtgagc taagcccttg tcacataaag aaaaatcccg ttttccggct
     1621 tgaccgggtg gttttttttc aatatagtgg gtctaagtgg taaaaagtgg gtgaaagtgg
     1681 gattaagtga tgtttcaagg atcgacacaa ctgagcgtag acagcaaggg caggctcgca
     1741 ataccggcaa ggtatcgcga catgctgctt gcgcattgtg caggcagcct ggtgctgacc
     1801 gccgatgccg acggctgcct gttgatctat ccccagcccg attggcagcc gatccgcgag
     1861 aagctgctga agctctcggc gctcaatccg cgcatccgtg ccttgcagcg tttgctggtc
     1921 ggtcacgccg aagaggtggt gatggatggc gcggggcggg tgctggtgtc gccgacgctg
     1981 cgcagttatg ccgcgctgga caagcacgtg atgctgatcg gccagggcaa caaatttgaa
     2041 ttgtgggacg aggccaagtg gcaggcgcag caggacatcg cgctgtcgct gatgagcggt
     2101 gagttgccgc cggagcttga gggcttttcc ttgtgagcga ggcggtggcc tccgagctga
     2161 cgcatgagac ggtgctgttg tccgaggcgg tggatgcgct ggaggtcaag ccggacggca
     2221 tttatgtgga cggcaccttc gggcgcggcg gtcacagtgc gcggatattg gcgcagttgg
     2281 ggccgaacgg caggttgatc gcgctggaca aggatccgac tgcggttgcg gtgggcaggc
     2341 aatggacgga tgcgcgcttc acgatggtgc accgcgggtt catcgagttg ccgcaggtgc
     2401 tggacgagtt gcaggtaaca caggtggacg gcgtcctgct ggatctgggg gtgtcgtcgc
     2461 cgcagctgga cgaggcggtg cgcggattca gttttcgatt cgatgcgccg ctggatatgc
     2521 gcatggacac cagcagcggg atgaccgcag cgcagtggct ggcgacggtg gatgaaggtt
     2581 tactcgcgga ggtgatacgt gattacggcg aagaacggtt tgctaagcag gttgcaaggg
     2641 cgattgttac ggcgcgcgca gtggagccta tcgaaaccac gcggcagctc gtcgagctgg
     2701 ttggcaaaac ggtacgtacc cgtgaagcag ggcagaaccc ggccacgcgg acctttcaag
     2761 ctatacggat ttatctcaac cgggagctcg aagagcttgc gggggtgttg ccagagtgcg
     2821 tgacgcgact caagccgggc ggacgtctgg cggtgatcag cttccattcg ctggaagacc
     2881 gcatcgtgaa gcatttcatg cgcgacatgg ctgagggtga caagttgccg cgcaatgtgc
     2941 cgatccgcgc cagcgaagtg ccgcaggggc ggatgcatct gatcggcaag gcggtgcgcg
     3001 ccacggcgga agaggtgcag gcaaatccgc gcgcgcgcag cgcggtgttg cgcatcgcgg
     3061 agcgcaacga tgtcgcgtag cgggaatggc ctgaatgtgc tgctgctggt ggcggtggtg
     3121 atgtgcgcgt tgagcgtggt cacttcgcag cacaaggcgc gcaagttgta tatcgagttg
     3181 cagaaggaaa aagaaagcgc gcagcagatg gatgtggagt gggggcagtt gcagttggag
     3241 ttgagcacct gggccacacc ggcgcgggtg gagaatattg cagcgcggca gctgcggatg
     3301 cagttgccgg caaatgggca agttcagttt ataagggttg agcctaacgg ccaaagagat
     3361 aaccagtcat gaaatatgcg gctagcgcta aaaaagacgt cccggcaaag ttgcccggat
     3421 ggcgcgccac cgcgttattc gtggcgttgc ttgcaggtct gctgggactg atcggtcgca
     3481 gcgcctatct gcaggtcatg cacgaagact ttttgcagga aaagggcaat gcccgctaca
     3541 gccgggtgat cgaggtcagc gcgcatcgcg gcatgatctc ggatcgccat ggcgagccgc
     3601 tggcggtcag cacgccggtg gaatcggtgt gggccagccc
//
LOCUS       DASUIV010000223         6033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_147800, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000223 DASUIV010000000
VERSION     DASUIV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147800"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFW59_08130"
     CDS             complement(<1..454)
                     /locus_tag="VFW59_08130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5364225.1"
                     /translation="MTQPANNPLSASEPWNLVADGYAETTMHVFEEFAEEAVAASRLK
                     PNSTLLDVACGPGTLALKVAQHAGQIHGIDFSESMLAVFRNKIERAGHRNIELHCGDA
                     QTLPYRDATFDAAFSLFGLMFFPDRQRGFSEIYRTLKPGGSIAITSWAP"
     gene            complement(708..1337)
                     /locus_tag="VFW59_08135"
     CDS             complement(708..1337)
                     /locus_tag="VFW59_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX5364226.1"
                     /translation="MSKYQFPASDIYLPGTDIPRNRPGIETPDLLHEVEATLLRQAYT
                     RFIAELAPSVLFDEEYFKALHRDTYESLYEWAGLYRTVDMSKGGSLFCRAAYLEQEAR
                     RIFRELEREQYLKQAADGTVEKFAERLAHYQSELIALHPFYELNGRITRLFFDLIAVY
                     NGYEPIDYSHALAEGPSGLNAYIEASILCVQRADSSKLQQLILQGLRRA"
     gene            complement(1309..1467)
                     /locus_tag="VFW59_08140"
     CDS             complement(1309..1467)
                     /locus_tag="VFW59_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364227.1"
                     /translation="MQISPSLNKKIAKAVQTSMRIEGCKPVQSDAIKQQALALMEQHR
                     VQVSVPRK"
     gene            complement(1624..2058)
                     /locus_tag="VFW59_08145"
     CDS             complement(1624..2058)
                     /locus_tag="VFW59_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5364228.1"
                     /translation="MTAENQVWSGSTSQITNLGTFILCGLLSLTIVLAIVALPYALWK
                     YLVVKNQKYELTSQRLKSRSGVLSKTTDELELYRVKDTKFEQSFFLRLFGLGNVLLIS
                     SDSTTPVTVIAAVKEAESLREQIRTLVEDRRGQMRVRVAELE"
     gene            complement(2175..2699)
                     /locus_tag="VFW59_08150"
     CDS             complement(2175..2699)
                     /locus_tag="VFW59_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364229.1"
                     /translation="MKHLFESVIPFLLYATLLSGCATEPMISRSVHTTAYPPTAAAKY
                     HIPELPDGYTRLYIYRPQTFIGMMDNPVITINGKPIGNPENPYENRFLPGALFVLDTP
                     GDIAQVSWIWAYDKTDRVLSLSSKESRIWYLRWHIPPLNFPGERYLEVTPQEIAAREI
                     EPLRFTGYVKLPAP"
     gene            complement(2745..3983)
                     /locus_tag="VFW59_08155"
     CDS             complement(2745..3983)
                     /locus_tag="VFW59_08155"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5364230.1"
                     /translation="MSQNPNPPAAFDIERIRRDFPILSREVHGKPLVWLDSAATAQKP
                     QAVIDRVSHFYEHENANIHRGTHALATEACAAYEGARGKLASFLNAASPQEIVFVRGT
                     TEGLNLLAATLGASLQPGDEIVLTEAEHHANIVPWQFTAQKTGAVIRVAPVDDGGDLI
                     LEEYERLLGPRTRIVSITHIANAIGTVMPIREMTQLAKRVGATVVIDGAQGAPHRRVD
                     VQAIGCDFYVFSGHKLFGPSGIGAVYGRRERWDELPPWQGGGGMIRTVTFEKSTFAHP
                     PKRFEAGTPATAAAVGLGAAIDYLNGIGIDAIEKYETDLLDYALQGILGVDGLRVIGM
                     PRDRGGVISLVMDGIPDQEVGKRLDLAGIAVRTGHHCAQPILARFGLESTVRPSFAFY
                     NTRAEADMLVETLRGIGLRR"
     gene            4060..4398
                     /locus_tag="VFW59_08160"
     CDS             4060..4398
                     /locus_tag="VFW59_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364231.1"
                     /translation="MQTKYISYSSTSRPGPLRRLAALVVTVALVALALMFSAVLLAVI
                     LIVGAIAGVYLWWKTRAIRKQFKQMQAQMQNASSRSADTQAFRAEAFGGEIIEGEAVR
                     VDQPDARIRH"
     gene            4404..4823
                     /locus_tag="VFW59_08165"
     CDS             4404..4823
                     /locus_tag="VFW59_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5364232.1"
                     /translation="MWLQREIQLTPKARGFHLITGELLHGLPELRDFRVGMMNVFILH
                     TSASLTLNENADPAVRQDFESWFNRAVPEDEPDYRHRDEGSDDLPAHIKSSLLGASLN
                     IPVGNGRPALGTWQGIYLCEHRNHGGSRRIVVTVQGE"
     gene            4823..5107
                     /locus_tag="VFW59_08170"
     CDS             4823..5107
                     /locus_tag="VFW59_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5364233.1"
                     /translation="MNDALKTLHLVMHGRVQGVWYRDSMRREAEHLGVSGWVRNRSDG
                     AVEATVQGEAGAVDALVRWAHRGPQLAEVERVDVEPGSGSYAGFEVRGSA"
     gene            complement(5133..5552)
                     /locus_tag="VFW59_08175"
     CDS             complement(5133..5552)
                     /locus_tag="VFW59_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5364234.1"
                     /translation="MSFMSWLVIGVLFMLGEIGTGNFYLLSIGLAFIYPAIAAYFDAP
                     TATQLTALGLGVMAHAMAVLILHQIRPSKSAANASADIGQRVEVIEWLDECSARVSYR
                     DREWQADKVYAEMPDAAYGIIKAVQGNRLTISTAETA"
     gene            complement(5628..>6033)
                     /locus_tag="VFW59_08180"
     CDS             complement(5628..>6033)
                     /locus_tag="VFW59_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5364235.1"
                     /translation="RFTLPTALNLALGIATVACHLHARGIMHGDLYAHNIQWNPQGDC
                     LLGDFGAASFLPQDAQQAAALQRIEVRAYACLLEELLERCDAPPEAAHLVTALQDLQH
                     HCDQAAVDERPLFADIRQQLAALQQGASRYGD"
BASE COUNT         1034 a         1695 c         2039 g         1265 t
ORIGIN      
        1 cgggcgccca actggtgatg gcgatgctgc cgcccggttt gagcgtgcgg taaatctcgc
       61 tgaagcctcg ttggcggtcg gggaagaaca tcaggccgaa tagcgaaaag gcggcgtcga
      121 aggtggcatc acgatagggc agggtctgcg cgtcgccgca gtgcagttcg atgttgcggt
      181 gtccggcccg ttcgatcttg ttgcggaata ccgcgagcat ggattcggag aagtcgatgc
      241 cgtgtatttg cccggcatgc tgcgccacct tgagcgccag cgtgcccggc ccgcaggcga
      301 catcgagcag ggttgagttg ggtttgagtc ggctggcggc gacggcttcc tcggcgaatt
      361 cttcgaacac atgcatcgtg gtttcggcgt agccgtcggc gacgaggttc cagggttcgc
      421 tggcggaaag ggggttgttg gctggctgcg tcatgttgat ttaatgcccg tggctcgact
      481 tcgtacaatt cggtctcttg agccgaccgc gcaatgtacc ccgtttaggc gaataaggaa
      541 cctgtccggg ttggcaggtt tatttcggga ggaatctgtg ctagctgccc gaaaaaatca
      601 gggcatggtt gatcccctgt ctgggggcgc ccctcctcaa gccgccaact gctgcgaggc
      661 caatcgcctg ggcgatcttc gcgcgctgga ggaaagcggg ttctggctca tgcgcgccgc
      721 aatccttgaa gaatgagctg ctgtaatttg ctgctgtcgg cgcgctgcac gcacaggatg
      781 gaggcttcga tgtaggcgtt caggccggac ggtccttctg ccagtgcatg gctgtagtcg
      841 atgggttcgt agccgttgta cacggcgatg agatcgaaga acaggcgggt gatgcgtccg
      901 ttgagttcgt aaaagggatg cagggcgatc agttcggact ggtaatgggc gaggcgttcg
      961 gcaaattttt ccaccgtccc gtctgcggcc tgtttcaggt attgctctcg ttccagttcg
     1021 cggaagatgc ggcgggcttc ctgttcgagg taggccgcgc ggcagaacag cgagccgcct
     1081 ttgctcatgt ccacggtgcg gtacaggccc gcccattcgt acagggattc gtaggtgtcg
     1141 cggtgcagcg ctttgaagta ttcctcgtcg aagaggacgg agggggcgag ttctgcgatg
     1201 aagcgggtgt aggcttgtcg gagcagtgtt gcttctactt cgtgcaacaa gtccggcgtt
     1261 tctattcccg ggcggtttct gggaatgtcg gtgccgggaa ggtagatgtc acttgcgggg
     1321 aactgatact tggacacgat gctgttccat cagggcaagc gcctgctgtt tgatggcgtc
     1381 agactgcacc ggcttgcagc cttcgatccg catcgaggtc tgcacagctt tggcgatttt
     1441 cttgttcagg ctggggctga tttgcatcgt tttctcccaa aagcgactcg ccagtcgagg
     1501 caagtattgt tgctgaaaat tatatcagtt ccggctaatc gagggctgca ggattaaata
     1561 gggcggctac cccagccgcg catcctcatt cggcgggttg ccgccgctgg ctgatcctgc
     1621 gcgtcactct aattccgcaa cgcgcacacg catctgtccc cgacgatctt cgaccagggt
     1681 tctaatttgc tcccgcaggg attcggcctc tttgactgcc gcgatgactg tcactggggt
     1741 ggttgaatcc gatgaaatca acaacacgtt tcccaggcca aatagccgca ggaagaacga
     1801 ctgttcaaac ttcgtgtcct tgacgcggta gagttccagt tcatccgtcg tcttggacag
     1861 cacgccgctg cgggacttca atcgttgcga ggtaagctca tacttctggt ttttgacaac
     1921 gagatacttc cacagcgcat aaggcagcgc gacgatcgcc agcacgatgg tgagcgacag
     1981 gagcccgcac agaatgaacg tgccgagatt ggtgatctgg gaggtgctgc cagaccagac
     2041 ttgattttct gcggtcatgt ttttaatttc ctttcgggtt gttgggcggc gtatccgttt
     2101 gggggcggtc gcggtttttt gagtatctca tcattcctcc tgcacaccca gcctcgcttc
     2161 ctgagccagc ctcctcatgg ggctggcagc ttgacgtagc cggtgaagcg caacggttcg
     2221 atttctcgtg ccgcgatctc ttggggtgtc acctcgagat agcgctctcc ggggaaattg
     2281 agtgggggaa tgtgccatct caagtaccag atgcgcgact ctttggatga taaagacaat
     2341 acccggtctg tcttgtcata tgcccagatc catgagactt gagcaatgtc gcccggagta
     2401 tccagtacga acagggcacc cggcaggaaa cggttctcat agggattctc cgggttgccg
     2461 atgggtttgc cattgatggt gataacgggg ttgtccatca ttccaatgaa tgtttgcggg
     2521 cggtatatat atagccttgt gtatccgtct ggcagttctg gaatgtggta tttcgccgca
     2581 gcagtgggag ggtaggcggt cgtgtgtacg ctgcgtgaga tcatgggttc ggtagcgcag
     2641 cccgataaca gcgtcgcata gagcagaaat ggaatgacgc tctcgaaaag gtgtttcatg
     2701 tttcttcctc ctctaatcag ggtattcgac cttcccggga agtgctaccg ccgcaggccg
     2761 atgccgcgca gcgtctcgac cagcatatcg gcctcggcgc gggtgttgta gaaagcgaac
     2821 gagggccgga ccgtggattc cagcccgaag cgcgcgagga tcggttgcgc gcaatggtgt
     2881 ccggtgcgca ccgcgatgcc tgccagatcc agccgtttgc cgacctcctg atccgggatg
     2941 ccgtccatca ccagcgagat gacgccgccc cggtcgcggg gcatgccgat cacgcgcagg
     3001 ccgtcgacgc cgagtatgcc ttgcagcgcg taatccagca gatcggtttc gtatttttcg
     3061 atcgcgtcta tgccgatgcc gttcaggtag tcgatcgcgg cacccagccc cacggccgct
     3121 gcggtggcgg gggtgcccgc ctcgaacctt ttgggcgggt gggcgaaggt gctcttctcg
     3181 aaggtgaccg tacggatcat gccgccccct ccctgccagg gaggcagctc gtcccatctc
     3241 tcgcggcggc cgtagacggc gccgatgccg gaggggccga acagcttgtg cccggagaac
     3301 acatagaagt cgcagccgat cgcctgcacg tccacgcggc gatgcggcgc gccctgtgcg
     3361 ccgtcgatca ccacggtcgc gcccacgcgc ttggccagct gcgtcatctc gcggatcggc
     3421 atcacggtgc cgatggcgtt ggcgatatgg gtgatcgaga cgatgcgggt gcgcggcccg
     3481 agcaggcgct cgtattcttc gaggatcagg tcgccgccgt cgtccaccgg ggcgacgcgg
     3541 atcaccgcgc cggtcttctg cgcggtgaat tgccagggca cgatgttggc gtggtgctcg
     3601 gcttcggtca gcacgatctc gtccccgggc tgcaggcttg cgcccagcgt cgcggcgagc
     3661 aggttgaggc cctcggtggt gccgcgcacg aacacgatct cctgcggcga agcagcgtta
     3721 aggaaggagg cgagcttgcc gcgcgcgcct tcgtaagcgg cacaggcttc cgtcgccagc
     3781 gcatgggtgc cgcgatggat gttggcgttc tcgtgctcgt agaaatgcga tacccggtcg
     3841 atcacggcct gcggcttctg tgccgtcgcg gcgctgtcca gccagaccag cggcttgccg
     3901 tgcacctcgc ggctcaggat cgggaaatcg cggcggatgc gttcgatgtc gaaggcggct
     3961 ggcgggttgg ggttctggct cacggtactt ccctcgtgga gaggagccca tggtacgcga
     4021 ctgtctgcgg cggcagcaag gatgggatag acttgcaaga tgcagacgaa atatatttcc
     4081 tattcctcga ccagccgccc cggcccgttg cgcaggctgg cggctttggt ggtgacggtg
     4141 gcgctggtcg ctctggcgct gatgttctcg gcggtgttgc tcgcggtcat cctgatcgtc
     4201 ggtgcgattg cgggtgttta cctgtggtgg aagacgcgcg cgatccgcaa acagtttaaa
     4261 cagatgcagg cgcagatgca gaacgcttcc tcccgtagcg cggatacgca ggcattccgc
     4321 gccgaggcgt tcgggggcga gatcatcgag ggcgaagccg tccgcgtgga tcagccggac
     4381 gcgcgcatca ggcactgagc gcgatgtggc tgcaaagaga aatacagctg acgcccaaag
     4441 cacggggctt tcatctgatc accggcgagc tgttgcatgg gctgcccgag ctgcgcgact
     4501 tccgcgtcgg catgatgaac gtcttcatcc tgcacacctc ggcatcctta acgctgaacg
     4561 agaacgccga tcctgcggtg cggcaggatt tcgagagctg gttcaaccgt gcggtgccgg
     4621 aggacgagcc tgactaccgc catcgcgacg agggttcgga cgacctgccc gcgcatatca
     4681 aatccagcct gctgggtgcg agcctgaaca tcccggtcgg caacgggcgc cccgcgctgg
     4741 gcacctggca gggcatctat ctgtgcgagc atcgcaacca cggcggcagc cgccgcatcg
     4801 tcgtgacggt acagggggaa tgatgaacga tgcgttgaag acgctgcatc tggtgatgca
     4861 tggccgcgtg cagggcgtgt ggtaccgcga ttcgatgcgc cgcgaggccg agcatctggg
     4921 cgtcagcggc tgggtgcgca accgcagcga cggcgcggtg gaggcgacgg tgcagggaga
     4981 ggccggtgcg gtggatgccc tggtgcgctg ggcgcatcgc ggcccgcagt tggccgaggt
     5041 ggaaagggtg gatgtcgagc cgggcagcgg cagctatgcg ggcttcgagg tgaggggttc
     5101 ggcatgaggc cggtgtcggg tggcgggttc ttctaggcgg tttctgcggt tgaaatggtg
     5161 aggcggttgc cctgcacggc cttgatgatg ccgtaggcgg cgtcgggcat ttcggcgtag
     5221 accttgtcgg cctgccactc ccggtcgcga tagctcactc tggcgctgca ttcatccagc
     5281 cattcgatca cctcgacgcg ctggccgata tccgcagagg cgttagcggc cgacttggac
     5341 ggcctgatct gatgcaggat cagcaccgcc atcgcgtgcg ccatgacgcc caggcccagt
     5401 gcggtcagct gtgtcgcggt gggggcatca aaataggcgg cgatggcggg atagatgaaa
     5461 gccagcccga tgctcagcag gtagaagttt ccggtgccga tctcgccgag catgaacaga
     5521 acgccgatca ccagccatga catgaagctc atacacattc tcctgaaacg ttaaacggct
     5581 tcaatcttgg ctgtggcggt gttttttgta aagcgggccg gcgcttttca gtcgccgtat
     5641 cgggaggcgc cttgctgcaa cgctgccagc tgttgccgga tgtccgcgaa cagcgggcgc
     5701 tcatcgacgg ccgcttgatc gcagtgatgt tgcagatcct gcagggcggt gacgaggtgc
     5761 gcggcttcgg gcggcgcgtc gcagcgttcc agcagctctt ccagcaggca ggcgtaggcg
     5821 cgcacttcga tgcgttgcag cgcggcagcc tgttgcgcgt cctgtggcag gaacgaggcg
     5881 gcgccgaagt cgcccagcag gcagtcgccc tgcggattcc actggatgtt gtgcgcgtac
     5941 aggtcgccgt gcatgatgcc ccgcgcatgc agatggcagg ccacggtggc gatgccgagg
     6001 gcgaggttga gcgcggtcgg cagcgtgaag cgg
//
LOCUS       DASUIV010000224         5674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1356589, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000224 DASUIV010000000
VERSION     DASUIV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356589"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VFW59_08185"
     CDS             <1..172
                     /locus_tag="VFW59_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HEX5364236.1"
                     /translation="NREKWDEYEHAVCDMVDRTSTEIAPWTLVEANDKNFARIKILKT
                     LCQQIEAAMKKK"
     gene            296..1300
                     /locus_tag="VFW59_08190"
     CDS             296..1300
                     /locus_tag="VFW59_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEX5364237.1"
                     /translation="MTRHHVLSLSIGMGALFAAIGTAQAAASKEPIQPIVAVKPKSAA
                     MVELGKKLYFDPRLSKSGFISCNSCHNLSMGGTDNLKTSIGHNWHQGPINAPTVLNSS
                     MNIAQFWDGRAADLKAQAGGPIANPGEMASTHELAINILQSIPAYTAEFKSAFGSDKI
                     DIDKVTTAIAAFEETLTTPDSRFDKWLKGDKKALSKDELAGYKLFKDSGCTACHNGPA
                     VGGNSFQKLGVVEPYKTTSTAEGRIAVTGKDADRFYFKVPTLRNVELTYPYFHDGEAA
                     TLKDAVNTMGRIQLGKSFTEDENSKIVAFLKTLTGKQPSFKMPILPPSNDNTPRPQPF
                     "
     gene            1375..2736
                     /locus_tag="VFW59_08195"
     CDS             1375..2736
                     /locus_tag="VFW59_08195"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat-containing protein kinase
                     family protein"
                     /protein_id="HEX5364238.1"
                     /translation="MQTLQSLRDGKLAGSKRLDLSCGLTRFPEEIFELADSLEILNLS
                     GNALSGLPDELPKLHKLRVIFCSDNRFTRLPDVLGQCPHLEMVGFKANQIASVPAAAL
                     PDKLRWLILTDNRITGLPARLGSCMRLQKLMLSGNRLQSLPDEMAACTNLELLRIAAN
                     RFEALPDWLFSLPRLAWLAYAGNPFSDACEVAAMATQPIAHIDWHALELQHQLGEGAS
                     GVIYRARLQTAGDEAAVAVKIFKGALTSDGLPRSEKAACIAAGRHPGLIPVAGKISGH
                     PEGTPGLVMALIDPSFRNLAGPPSLASCTRDIYPAETEFSLASALNIACGIAAVAEHL
                     HAQGVMHGDLYAHNILRNEKGDCLLGDFGAASFLPNSSDFAQQDAHPAAALQRIEVRA
                     FGCLLEELLERCDAPVEARDILEQLRALQQRCDQSEPAGRPLFGEIRNTLQSLRGEAA
                     IAR"
     gene            complement(2753..3058)
                     /locus_tag="VFW59_08200"
     CDS             complement(2753..3058)
                     /locus_tag="VFW59_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2288 domain-containing protein"
                     /protein_id="HEX5364239.1"
                     /translation="MTSPNQQEIYRAKVNLETSQIAWKELQRFFASGAALVVSRELDL
                     VEAAFQMSQDNAAQIRQWMDEGKFGKVTDEQALAWYEADAVMWAVVVSPWVLVQPSE"
     gene            complement(3162..3701)
                     /locus_tag="VFW59_08205"
     CDS             complement(3162..3701)
                     /locus_tag="VFW59_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5364240.1"
                     /translation="MPIFSYLDTSPDLGDRVYLHASCQVLGDVKIGDDSSVWCNTVLR
                     GDVNRIVIGRGSNVQDLSVGHVSHKTADKPEGSPLIIGDYVTVGHSVILHGCTIGNEC
                     LIGMGSIVMDDAVIPDRVMIGAGSLVSPGKTLESGMLYMGRPAKAVRALTEEEIAYLR
                     YSAEHYMRVKDNYLNTPTR"
     gene            complement(3828..4880)
                     /gene="rsgA"
                     /locus_tag="VFW59_08210"
     CDS             complement(3828..4880)
                     /gene="rsgA"
                     /locus_tag="VFW59_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX5364241.1"
                     /translation="MELTELGLDRGLAEMATCGAGQRLARVTAVDRGRYVVRDEHGEV
                     PAEPTGKYLHAAASSVDMPCVGDWVCVQYHDTGSHATIHDLVPRRSFLRRKSPGKNID
                     FQMIAANIDVAFIVQSCHFDFNVRRLERYLVMVNEGHIEPVLLLTKTDLISEAELERM
                     IGIIRAAGITARIIALSNVTGEGVTQVREVMEAGKTYCLLGSSGVGKTTLINQLLGHD
                     ILETGDVSGTGEGRHTTTRRHLVVLENGALLIDMPGMRELGMLSTGEGLNDSFVDIGE
                     LATRCRFADCSHTNEPGCAIREAIERGELDAEHYQSYLKLKAESEFNEMSYLDKRKKD
                     KAFGKMVKSVLKDRRK"
     gene            complement(4969..5277)
                     /locus_tag="VFW59_08215"
     CDS             complement(4969..5277)
                     /locus_tag="VFW59_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX5364242.1"
                     /translation="MSKTAKLRNIHPGEVLREEFLIPLGITQYRLAKEIGVTEARISA
                     ICSGKRAITADTALRLAAFFGTSSGFWMGLQADYDTEEAAKELSGVLAQIHRFEPLAA
                     "
     gene            complement(5290..5571)
                     /locus_tag="VFW59_08220"
     CDS             complement(5290..5571)
                     /locus_tag="VFW59_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5364243.1"
                     /translation="MITDFHDEETLKVWMGEFSRRLPNQIQDVARRKLRMLHNAQRIE
                     DLRIPPNNRLEALKGDRAGQWSIRINDQWRVCFKWNEGQASRVEICDYH"
BASE COUNT         1157 a         1785 c         1596 g         1136 t
ORIGIN      
        1 caaccgcgag aaatgggacg agtacgaaca cgccgtatgc gacatggtgg atcgcaccag
       61 caccgaaatc gcgccgtgga cgctggtgga agccaacgac aagaacttcg cgcgcatcaa
      121 gatcctgaaa acgctgtgcc agcaaatcga agcggcgatg aaaaagaaat agcaagggaa
      181 ctttagccgg gcagcactct cgaaccggcc tgcttttcaa aagcgggccg ctctcctcgc
      241 gagggtcggc gactatttga tgcaactcgt caccaaccac aaaaaggaaa atatcatgac
      301 cagacatcat gtgctgtcac tgagtattgg catgggggcc ttgtttgccg ctatcggcac
      361 agcgcaggcc gccgccagca aagaacctat ccagcctatc gtcgcggtca agcccaagag
      421 cgccgcaatg gtcgaactcg gcaagaaact ctacttcgat ccgcgtttgt cgaagtccgg
      481 ctttatctcc tgcaactcct gccacaacct gtccatgggc ggcaccgaca acctcaagac
      541 ctcgatcggc cacaactggc atcaaggccc gatcaacgcg cctaccgtgc tgaactccag
      601 catgaatatc gcccagttct gggacggtcg tgcggcagac ctcaaggcgc aagccggcgg
      661 ccctatcgcc aatccgggcg agatggcatc cacgcatgag ctcgcgatca atatccttca
      721 gtccatcccg gcttacacag cggaattcaa gagcgctttc ggctccgaca agatcgatat
      781 cgacaaggtg accacggcta tcgcggcttt cgaagaaacg ctgacaacgc ctgactcccg
      841 tttcgacaag tggctgaaag gcgacaagaa ggcgctgagc aaggacgaac tcgccggcta
      901 caagctgttc aaggatagcg gctgcaccgc ctgccacaac ggccctgcag tgggcggcaa
      961 ctccttccag aagctgggcg tggtcgagcc gtacaagacc accagcacag ccgaagggcg
     1021 cattgcggta accggcaagg atgcggatcg cttctatttc aaggtgccga cactgcgtaa
     1081 cgtcgaactg acgtatccgt acttccacga cggcgaagca gccacgctca aggacgcggt
     1141 caacaccatg ggtcgcatcc agttgggcaa gagcttcacc gaagacgaaa acagcaagat
     1201 cgtcgccttc ctgaagacgc tgaccggcaa acagccatcg ttcaagatgc ctattttgcc
     1261 gccatcgaac gacaacacac cacgtccgca acctttctga taacaagcgt tacggacatt
     1321 cttcgggggc ctctggcccc cgttttgttt ccgcaaccgg tatagttgcg ccctatgcaa
     1381 actcttcaat cgctgcgcga cggcaagctg gccggcagca aacgtcttga cctgtcctgc
     1441 ggcctgaccc gctttcccga agaaatcttc gaacttgccg actcgctgga aattctcaac
     1501 ctgtccggca acgcgttatc cggcttgccc gacgagctgc cgaagctgca caaactgcgc
     1561 gtgatcttct gttcggacaa ccgctttacc cggctgcccg acgtgctggg gcagtgcccg
     1621 catctggaga tggtcggctt caaggccaac caaatcgcca gcgtgcccgc tgccgcactg
     1681 ccggacaaac tgcgctggct gatcctgacc gataaccgga ttaccggcct gcccgcccgg
     1741 ctcggctcat gcatgcgcct gcaaaagctc atgctgtcgg gcaaccgctt gcaaagcctg
     1801 cccgacgaga tggccgcctg cacaaacctc gaattgctgc gcatcgccgc gaaccgcttc
     1861 gaggcattgc cggactggct gttctcgctg ccccgcttgg catggctggc ctatgcgggc
     1921 aacccgttca gcgatgcctg cgaagtcgcg gcgatggcaa cgcagccgat cgcgcacatc
     1981 gattggcacg cgctggagtt gcagcaccaa ttgggcgaag gcgcgtccgg cgtgatctac
     2041 cgcgccaggc tgcaaacggc tggcgacgag gcagcggtgg cggtcaaaat tttcaaggga
     2101 gcgctgacca gcgacggcct gccgcgtagc gagaaagcgg cctgcatcgc cgcaggtagg
     2161 cacccggggc tgatcccggt cgcgggcaag atcagcggtc atcccgaagg tacaccgggg
     2221 ctggtgatgg cgctaatcga cccgagcttc cgcaacctcg cgggcccgcc tagcctcgct
     2281 tcctgcacgc gcgacatcta tccggcggag actgaattta gcttggcatc cgcgctgaac
     2341 atcgcctgcg gcatcgcggc ggtcgcggaa catctgcatg cacaaggcgt gatgcacggc
     2401 gatctgtacg cgcacaacat tttgcggaat gaaaaaggcg actgcctgct gggcgacttc
     2461 ggcgcggcat cttttttgcc gaattcgtca gactttgcgc aacaggatgc gcatcccgcc
     2521 gccgcgttgc aacgcatcga agtgcgtgcc ttcggttgct tgctggagga gttgctggag
     2581 cgttgcgatg cgcctgtcga agcgcgggat attctggaac aactgcgcgc cttgcagcaa
     2641 cgctgcgatc agtccgagcc agccgggcgc ccgctgttcg gcgagatacg gaacacgctg
     2701 caatcgctgc gcggagaggc ggccatcgcc cgatagagcg cggcttttcg gattattcgg
     2761 agggctgcac cagcacccac ggactgacca ccaccgccca catcaccgca tccgcctcgt
     2821 accacgcgag ggcctgctca tcggtcacct tgccgaactt gccctcatcc atccattgcc
     2881 ggatttgcgc cgcattgtcc tgcgacatct ggaatgctgc ctcgaccaga tcgagctctc
     2941 tggaaaccac cagcgccgcg ccgctggcga agaagcgctg caactctttc caggcgattt
     3001 gcgaggtttc gaggttgacc ttggcgcggt agatttcttg ttggttcgga gaagtcattt
     3061 caatatttca atatttagtc agtaaaagag gctgtgtagg agcgggccct gcccgcgatc
     3121 actcagaatg tcgcgggcag ggcccgctcc tacacgctgt tttaacgcgt cggcgtattc
     3181 aaataattat ccttcacgcg catgtaatgc tcggctgagt agcgcaggta agcgatctct
     3241 tcttccgtca gcgcgcgcac cgccttggcg ggacggccca tgtagagcat gccgctctcc
     3301 agcgtcttgc ccggcgacac cagactgcct gccccaatca tcacgcggtc gggaatcacc
     3361 gcatcgtcca tcacgatgga gcccatgccg atcaggcact cgttgccgat ggtgcagccg
     3421 tgcaggatta ccgaatggcc gaccgtcaca tagtcgccga taatcagcgg tgacccttcc
     3481 ggcttgtccg ccgtcttgtg cgacacgtgg cccacgctca gatcctgcac attgctgccg
     3541 cgcccgatca cgatgcggtt cacatcgccg cgcaacacgg tgttgcacca gacggaactg
     3601 tcgtcgccga ttttcacgtc gccgaggacc tggcaggaag cgtgcagata gacgcggtcg
     3661 ccgaggtcgg gggatgtatc gagataagaa aaaattggca tagcggttat aaactttccg
     3721 gccacatcct agccgtatgg atgacgatac gtttgttaag tgctcatgct ttcatctgtc
     3781 ggaaaaataa ttgcgatgca gccctcttta atttcggcgg gattatctca cttccgcctg
     3841 tccttcagca ccgacttcac catcttgccg aaggccttgt ccttcttgcg cttgtccaga
     3901 taagacatct cgttgaactc cgactcggcc ttgagcttga ggtagctctg gtaatgctcc
     3961 gcatccagtt cgccgcgctc tatcgcctcg cggatcgcgc agcccggttc gttggtgtgg
     4021 ctgcaatcgg cgaaacggca gcgcgtggca agttcgccga tatcgacgaa gctgtcgttc
     4081 agaccttctc cggtgctcag catgcccagt tcgcgcatgc ccggcatgtc gatcagcagc
     4141 gcgccattct ccagaacaac aaggtggcgg cgcgtggtgg tgtgccgccc ctcgccggtg
     4201 ccgctgacgt ctccggtctc cagaatgtcg tggcccagca gctggttgat cagcgtcgtc
     4261 ttgcccacgc ccgaagagcc gagcaggcaa taggtcttgc ccgcttccat cacctcccgc
     4321 acctgcgtca cgccttcgcc agtcacattg ctcagcgcga tgatgcgcgc cgtgataccc
     4381 gccgcgcgga tgatgcctat catgcgctcc agctccgcct cgctgatcag gtccgtcttg
     4441 gtcagcagca gcaccggctc gatatgcccc tcgttgacca tcaccagata gcgctccagc
     4501 ctgcgcacgt tgaaatcgaa atggcacgat tgaacgatga acgccacatc gatgttcgcc
     4561 gcgatcatct ggaaatcgat gttcttgccc ggagacttgc gccgcaggaa agaccttctc
     4621 ggcaccaggt cgtggatggt cgcgtgcgac cccgtatcgt gatactgcac gcagacccag
     4681 tcgcccacgc agggcatgtc caccgaggat gcggcggcgt ggaggtactt gcccgtcggc
     4741 tcggcaggca cctcgccatg ttcatcgcgg acgacatagc gcccgcggtc caccgcagtt
     4801 acccgggcca ggcgctggcc tgctccacac gttgccatct ccgcaagccc ccgatccagt
     4861 ccgagttcgg tcagctccat ggtgatcctg tctctaattc agaaaagaac atcgcccttg
     4921 cgggcgacgt gtaagaagct agtgcgatgc tacgaccatt gttcgcaatc aagcggcaag
     4981 tggttcgaaa cgatgtatct gcgccaacac cccagacaat tcttttgcgg cttcctcggt
     5041 atcgtagtcc gcctgcaagc ccatccagaa gccgctcgaa gtgccgaaga acgcagccag
     5101 gcgcaaggcg gtgtccgccg ttattgcgcg cttgccggag cagatcgccg agatgcgcgc
     5161 ttcggtcacg ccgatctctt tggccagacg atattgggta atgcccaggg ggatcaggaa
     5221 ttcttcccgc agcacttcgc cgggatggat gttgcgcagt ttggctgttt tgctcatggc
     5281 tgttactcct cagtgataat cgcagatttc cacgcgacta gcttgccctt cattccattt
     5341 aaagcacacc cgccattgat cgttgatgcg gatactccac tgtccggcgc gatcgccttt
     5401 caatgcctcc agccgattgt tcggtgggat acgcaaatcc tcgatgcgct gcgcgttgtg
     5461 caacatgcgt aacttgcgtc gcgccacgtc ctgaatctgg ttaggcaggc ggcgggaaaa
     5521 ttcacccatc cagaccttga gtgtctcttc gtcgtggaag tcggtaatca tgggaagtaa
     5581 tgctatcgct gtgcgatagt atcgtcaagg ggcgctcaaa agcgccctgt tggagggagg
     5641 cctacttgaa aatttaatgg tgatgctgtc ccat
//
LOCUS       DASUIV010000225         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1265195, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000225 DASUIV010000000
VERSION     DASUIV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265195"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1166
                     /locus_tag="VFW59_08225"
     CDS             132..1166
                     /locus_tag="VFW59_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HEX5364244.1"
                     /translation="MEYRKLGSSDLMVSALSLGTMTFGEQNSEAEAHAQLDYAVSRGV
                     NFIDTAEMYPVAPRAETVHRTETYIGPWLKRQQRDKLIVATKIAGPGRGFGWIRNGPR
                     IDREHLHAAIDASLSRLQTDYVDLYQSHWPDRYVPMFGATSYDVTQERDSVPVAEQLQ
                     ALAELVKAGKVRHIGLSNETPWGVAEFVRCAEQFGLPKIVSIQNSYHLMNRTFESGLS
                     EVCHHANVGLLAYSPLAFGHLTGKYLDNPQARGRINLFPNFGQRYNKVNVPAASAEYV
                     RIARTAGLSPAQMALAYARTRWFTASVILGATSLAQLEENLDSMDVTLPAELLEQIEA
                     THRRYFNPAP"
     gene            1257..>2137
                     /locus_tag="VFW59_08230"
     CDS             1257..>2137
                     /locus_tag="VFW59_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX5364245.1"
                     /translation="MVNRKKAQPASSASPQPPAPQAGEFPIVGLGGSAGGLEAFEQFF
                     RNIPSDSGMAFVLVSHLDPNRASILTEILQRNATIPVLEAQDQMSVQPNHVYVIPPNR
                     DLSIFHGKLLVTVPTMPHGQRMPIDGFLRSLSEDMADRAIGIILSGTGTDGTLGLRAI
                     LGGGGISLVQDPDTAKYDGMPGSAIKAGYATHILPVEKMPGVLLSGSRIQIRQQERQL
                     PQTATSGINRILMLLRATTGHDFSQYKKSTIGRRIERRMSMHEIEDTEVYARYLKEHP
                     AEIHVLFKELLINVTSF"
BASE COUNT          473 a          706 c          581 g          377 t
ORIGIN      
        1 ttaactggtt tcgtgccttt gcgagctttt gcctagcggc aaaattcctg cttaccccgc
       61 ttcgtgtttt tcgtggacaa acagatttat aaattcaaac gaaaactacc gattccacct
      121 aaggactgaa catggaatac cgcaaacttg gcagcagcga cctgatggta tcggccctga
      181 gcctgggcac gatgactttc ggcgaacaga acagcgaggc ggaggcgcat gcgcagctgg
      241 actacgccgt gtcgcgcggc gtgaacttca tcgacaccgc cgagatgtat ccggtcgcac
      301 cgcgtgccga gaccgtgcat cgcaccgaga cctatattgg cccatggctc aaacgccagc
      361 agcgcgacaa gctcatcgtc gccaccaaga tcgcaggtcc agggcgcggc ttcggctgga
      421 tacgcaacgg cccgcgcatc gaccgcgaac atctgcatgc cgcaatcgac gcgagcctga
      481 gccgcctgca gaccgactat gtcgacctgt accagagtca ctggccggat cgctatgtgc
      541 cgatgttcgg cgcgaccagc tatgacgtga cgcaggagcg cgacagcgtg cccgtcgccg
      601 aacaacttca ggcgctggcg gaactggtca aagcgggcaa ggtgcgccac atcgggctgt
      661 ccaacgagac accctggggc gtggccgaat tcgtacgctg cgcggaacag ttcggcctgc
      721 cgaagattgt ctcgatccag aactcctacc atctgatgaa ccgcaccttc gagagcggcc
      781 tgtccgaagt ctgccatcac gcgaacgtcg gtctgctcgc ctacagcccg ctggcattcg
      841 gccatctcac cggaaagtat ctggacaatc cgcaggcacg gggacgcatc aacctgttcc
      901 ccaacttcgg ccagcgttac aacaaggtca acgtccctgc cgccagcgcc gaatacgtac
      961 gcatcgcgcg cacagcaggc ctcagcccgg cgcagatggc gctggcctac gctcgcaccc
     1021 gctggttcac cgccagcgtc atcctaggcg cgaccagcct ggcgcaactc gaagagaatc
     1081 tggacagcat ggatgtcacg ctgccggccg aactgctgga gcagatcgag gcgacccacc
     1141 ggcgctattt caatccggcg ccctgacccc acacaccaga gaaacagccc accaccagcc
     1201 ccctcaatct gtgtaaaatc gctcgccatg tgcaattacc aatcgtaagg gtatacgtgg
     1261 tcaatcgtaa aaaagcacaa ccagcatcct cagccagccc tcagccgcca gcacctcagg
     1321 ctggcgaatt ccccatcgtc gggctgggcg gctcggcagg aggactggag gccttcgaac
     1381 agttcttccg caatatcccg tccgacagcg gcatggcctt cgtcctggtc tcgcatcttg
     1441 atccaaaccg cgccagcatc ctcaccgaaa tcctgcaacg caatgccacc atcccggtgc
     1501 tcgaggctca ggatcagatg agcgtgcagc ccaatcatgt ttacgtgatc cctcccaacc
     1561 gtgatctctc gatatttcac ggcaagctgc tggtgaccgt acccactatg ccgcacggac
     1621 agcgcatgcc catcgatggc tttctgcgct ccctgtccga ggatatggcg gaccgtgcga
     1681 tcggcatcat tctgtccgga accggcaccg atggcacgct agggctgcga gcgatactag
     1741 gcggcggcgg catctccctg gtacaggatc cggacacggc caaatacgat ggcatgccgg
     1801 gcagcgccat caaggcaggc tatgccaccc atatcctgcc ggtggagaag atgccgggag
     1861 tgctgctgtc cggctcccga atccagatca gacaacagga aagacaactt ccccagactg
     1921 cgaccagcgg catcaatcgc atcctgatgc tgctgcgcgc caccaccggg cacgattttt
     1981 cgcagtacaa gaagagcacc atcggtcgcc gcatcgaacg gcgcatgtcc atgcacgaga
     2041 tcgaagacac cgaggtctat gcacgctacc tgaaagaaca tccggccgag atacatgtgc
     2101 tgttcaagga gctgctgatc aacgtcacca gcttctt
//
LOCUS       DASUIV010000226         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_241841, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000226 DASUIV010000000
VERSION     DASUIV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241841"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFW59_08235"
     CDS             <1..180
                     /locus_tag="VFW59_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364246.1"
                     /translation="GLDFLNELERRRQPPLRAVILTGETSSQFIAHISNLPWAVLHKP
                     VNYVRLATCLQSGSA"
     gene            272..535
                     /locus_tag="VFW59_08240"
     CDS             272..535
                     /locus_tag="VFW59_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HEX5364247.1"
                     /translation="MPTDLSTLLGYPAAFLTTAAFVPQAWQSWRTRDLSGISLPMYLM
                     FTLGVALWLGYGLAIGSAPIIAANTVTLLLASVVLWLKIVERS"
     gene            complement(567..1223)
                     /locus_tag="VFW59_08245"
     CDS             complement(567..1223)
                     /locus_tag="VFW59_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX5364248.1"
                     /translation="MSTLPVLSLLETRVLGVLAEKQRTVPDSYPLTLNALVSGCNQKS
                     SRDPVIEAADAEVLAALDALRGLSLIVETSGGRAMRYGHNIERVLHIPSQSAALLAML
                     MLRGPQTAGELRLNCERLHRFADISSVESFLVELSEREAGALVAELPRQPGARENRWT
                     HLLSGEPVIEEFVRASSGDVTVGEIAALKANVARLETEVDELRAMLGRLCAELGVSLG
                     "
     gene            1477..2634
                     /locus_tag="VFW59_08250"
     CDS             1477..2634
                     /locus_tag="VFW59_08250"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5364249.1"
                     /translation="MASYTLYYATNRNHIGNDRWHPDTYGTKFSDDGMENLRFGTATI
                     NADEKIIKKHLNANMKDCGAGDGEKLGVYLAKCAEHSQITAYEESISDRIADTAQKTA
                     KFGSHAMFSDVKTAMKNSSDVLIYIHGFNVSWNEAVGSALALQIMLQNAPTKDKEQDV
                     LVILFTWPSDGMALPWASYKSDRSEATGSGAAVGRALLKARDFLADLRDRAKKGGSEL
                     CGQDIHLLCHSMGNFLLQSALARIFDHTPGNTLPRIFEHVFLCAADVDDTALETEQPL
                     GIVAQIARHVSIYYNREDKAMWVSDYTKGNPERLGHTGAAHPALLHNKIHQVDCTPIV
                     HGIVEHSYYLAGNINADIRASIDGWEQEDPRRLRIRNATLNNVWAMKKLSE"
BASE COUNT          650 a          910 c          771 g          492 t
ORIGIN      
        1 gggctggatt tcctgaacga actggagcgt cggcggcagc ccccgctgcg agccgtcatc
       61 ctcaccggcg aaacctcctc ccagttcatc gcccatatct cgaacctccc ctgggcggtg
      121 ctgcacaaac cggtcaatta cgtcaggctc gcaacctgcc tgcagtcagg ctcggcctga
      181 cggcgagcct catcccgacc acggccagac ctcgacaagc cgcccgcgta cgataaaatg
      241 cgccgcttgc cagcccgcca taacccgaac gatgcccacc gacctcagca ccctgctcgg
      301 ctatcccgcc gccttcctga ccaccgcagc cttcgtgccg caggcctggc agtcgtggcg
      361 cacgcgcgac ctgtccggca tctcgctgcc gatgtatctg atgttcacgc tgggcgtggc
      421 actgtggctg ggctacgggc tggcgatcgg cagcgccccc atcatcgcgg cgaatacggt
      481 gacgctgttg ctggcctcag tggtgctgtg gctgaagatc gtggaacgca gctagtcgcc
      541 cgcaccggcg aacagatacg cagagactag ccgagcgaca cgccgagttc ggcgcacagc
      601 ctgcccagca tcgcacgcag ctcgtccacc tccgtctcca gccgggccac gttggccttg
      661 agcgcggcga tctcgcccac cgtcacgtcg cccgatgaag cccggacgaa ctcctcgatc
      721 accggctcac cggagagcag atgggtccag cggttctcgc gcgcaccggg ctggcgcggc
      781 aactccgcca ccaaggcacc cgcctcgcgt tcggacagtt ccaccagaaa actctcgacc
      841 gaagagatgt ccgcgaacct gtgcagccgc tcgcaattga gccgcaactc tcccgctgtc
      901 tgcgggccgc gcaacatcag catggccagc aaggcggcgg actgagaggg gatatgcaac
      961 acccgttcga tgttgtgccc gtaacgcatc gccctgcccc cgctggtctc cacgatcaac
     1021 gacagcccgc gcagcgcgtc cagcgccgcc agcacctccg cgtcggcagc ctcgatcacc
     1081 ggatcgcggc tgctcttctg gttgcaaccg gaaaccagcg cattcagcgt cagcggataa
     1141 gagtccggca cggtgcgctg cttctcggcg agcacgccta ggacacgggt ttccagcagg
     1201 gagaggacgg gcaaggtaga catcgatgcg gtgcggaggt tgaggagagc cgattatgcg
     1261 aggcggcaca gcgcaggtca atttcaagca gccgaatcgc aaccacaaaa acgtccgcgc
     1321 gcccagctac caaccttggc aggtagtcag ccccccgggg gctggcatag cattttcagc
     1381 gtcacgaagg aaacaccgcc gtgagcaggg ctcaggaatg atgtctgcct tctccgattg
     1441 cccgcatctt ttgaacgtta tcgaaagggg aatcacatgg ccagttacac gctctactac
     1501 gcgaccaacc gcaatcacat tggcaacgac cgttggcatc ctgacacata cggaacaaag
     1561 ttcagcgatg atggcatgga gaatctccgc ttcggtactg caaccataaa cgcagatgaa
     1621 aaaatcatca aaaagcatct gaacgccaac atgaaagact gcggcgcagg agacggagaa
     1681 aagcttggag tgtatctggc gaaatgtgcg gaacactcac agatcacagc ctatgaagaa
     1741 agcattagcg accgcatcgc agatacggcc cagaagacag caaaatttgg ctcgcacgcg
     1801 atgttttcag atgtaaaaac cgcgatgaaa aacagcagcg atgtgctcat ctacatacat
     1861 ggcttcaatg tctcgtggaa tgaagccgtt gggtcggcac tggctttgca aatcatgctg
     1921 cagaacgccc ccacaaaaga caaagaacaa gatgtcctcg tcatattgtt cacctggcca
     1981 tccgacggaa tggcgctacc ctgggcctca tacaaatcag accgctccga agccaccgga
     2041 tctggtgcgg cagttgggcg cgcactcctg aaggcgcgcg acttcctcgc cgacctgcgc
     2101 gaccgcgcaa aaaaaggagg cagcgaacta tgcgggcaag acatccattt actctgtcat
     2161 tccatgggca acttcctgct gcagagcgcc ctggcacgga tattcgacca cactccgggc
     2221 aacaccctgc cgcgaatctt tgaacatgtt ttcctgtgtg ccgcagacgt cgatgacacg
     2281 gcattagaaa cagaacaacc gctgggaata gtcgcgcaga tcgctcgcca tgtttcgatc
     2341 tactacaacc gtgaagacaa ggcgatgtgg gtgtcagact acaccaaggg caacccggaa
     2401 agactcgggc ataccggggc agcgcatcct gcgttattgc acaacaagat tcatcaagtc
     2461 gactgcacgc ccatcgttca cggcatcgtt gagcacagct actaccttgc cggcaacatc
     2521 aacgccgaca tccgcgcaag tatcgacggc tgggagcagg aagaccctcg tcgactgcga
     2581 atacgcaacg caaccctcaa caatgtctgg gcgatgaaaa aactgtctga ataaattccc
     2641 gatttcctct gcttttcgga gaaaagccca tgcgaaccct gaagatcaat atcgtcatct
     2701 tgtcgaccat cctgtgcctt gccgccgtta cgctggagca ctcgatcgca gggagtcccc
     2761 tgttcgcctc gtcgaacatc ggatagatgc tgcccagctc aaggcagagg aactggccgc
     2821 gat
//
LOCUS       DASUIV010000227         9647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_985891, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000227 DASUIV010000000
VERSION     DASUIV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985891"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..734
                     /locus_tag="VFW59_08255"
     CDS             114..734
                     /locus_tag="VFW59_08255"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HEX5364250.1"
                     /translation="MSYILDALKKSDQQRQRGMTPTLPEAQPTLAAPRRTVLGAYGVF
                     AVVLLGAGIAIGWLHPWQAERVAPADEVLAVRSAITVPRPAILAPVSSEANGNTAREW
                     LAQNSTPGVPSSRKADIPVPPADSPREQPVISMAELPLAIRQEIPAMTIQLHAYSSQP
                     KNRLVSINSRMLHEGELLVQDLKLEQITPDGMIFSYKGYRFQRGIR"
     gene            complement(869..959)
                     /locus_tag="VFW59_08260"
     tRNA            complement(869..959)
                     /locus_tag="VFW59_08260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(923..925),aa:Ser,seq:gga)
     gene            complement(1081..1842)
                     /gene="gloB"
                     /locus_tag="VFW59_08265"
     CDS             complement(1081..1842)
                     /gene="gloB"
                     /locus_tag="VFW59_08265"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX5364251.1"
                     /translation="MLTITAIPALRDNYIWALHNDSHAALVDPGEAAPALDFLAAQGL
                     KLDAILCTHRHHDHIGGIAKLREVYNVPVYGRRHPYNPHITHDLREGERLRLDTLAAT
                     FDILDLPGHLDDHIAYLAPGMLFCGDVLFGAGCGRNFEGTLAQLHHSLQRLAQLPDDT
                     RVYCAHEYTEANLRFALACEPGNPELLQRIATTRQLRDAGLPSIPTTIALEKATNPFL
                     RCNAPEILHTLQAQGITDTSELGAFTALRQWKNEF"
     gene            1895..2641
                     /locus_tag="VFW59_08270"
     CDS             1895..2641
                     /locus_tag="VFW59_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5364252.1"
                     /translation="MSEWFASEQGAYVLAREQAYFDRTVADIFGFNALQLGLPEHGFL
                     RDSRMPLRFSAGNQAGNDVRLCCAELSFATASLDLVLLPHVLEFSDHPHQILREVERV
                     LMPEGNLIISGFNPRSLWGLHRAVGRRQGYPWCGHFIALSRLKDWLALLGFEVVGGRF
                     AAYAPPVYQAKWLERFAFMERAGDRWWAVSGGVYFLHAVKRVHGMRLIKPQWNEGLVS
                     KLMPAAPKLNNRITQHTGIDPRAHKEHLEN"
     gene            2647..3135
                     /gene="rnhA"
                     /locus_tag="VFW59_08275"
     CDS             2647..3135
                     /gene="rnhA"
                     /locus_tag="VFW59_08275"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX5364253.1"
                     /translation="MDKEIVDIFTDGACKGNPGVGGWGALLRVKGKERELFGGEAHTT
                     NNRMELLGAISALEALKRHCKVRLHTDSKYVQQGISTWIHGWKLRGWKTADRKPVKNE
                     DLWRRLDAVAAQHQIEWVWVKGHAGHDGNERADELANRGVLSAAENRLAGHPQGGEQC
                     AG"
     gene            3123..3824
                     /gene="dnaQ"
                     /locus_tag="VFW59_08280"
     CDS             3123..3824
                     /gene="dnaQ"
                     /locus_tag="VFW59_08280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX5364254.1"
                     /translation="MRRIVLDTETTGLNPQQGHRIIEVAAIVLEGRRVSKRHFHHYVD
                     PEREVDEEAARVHGFTWDMLRGRAKFADIAAGLLEFIGDSELIIHNAPFDMGFLNNEL
                     ALLGLPPLRNPVLDTLKLARDMHPGKKNNLDALCSRYEIDNAHRSLHGALLDTELLAE
                     VYIAMTRGQESLLGDDSTPSERQAALQELDGERPKLRVLQPTEEELAAHVQQLADIDK
                     ASKGACLWQQLEGAA"
     gene            3821..4669
                     /locus_tag="VFW59_08285"
     CDS             3821..4669
                     /locus_tag="VFW59_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5364255.1"
                     /translation="MSRWRRIAAGVVVVSMAGYLAACGYMWSQQKHLVFEPSPVIQTT
                     PARLGMKFEALRIPVGSGDERGELAAWWVPAERAGAATILYFHGNYRNIGNNLDHTRR
                     LHDLGYNVLLADYRGFGESTGGQPSEARVYEDAEAAWQYLLKVRGIDPRQAFIYGHSL
                     GGAVAIELAVHHPEAAGLITESTFTSMQAMGELEYGYLPVGLILNQRFDSLQKIAALK
                     LPVLLIHGSWDKKIPAAMAQQLYAAAPQPKRLTLIEGGEHNDSGMVGWVEYRDAVSAF
                     VGQYAH"
     gene            4706..5920
                     /locus_tag="VFW59_08290"
     CDS             4706..5920
                     /locus_tag="VFW59_08290"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5364256.1"
                     /translation="MKIDVIIIGAGAAGLMCAMQAAQRGRAVVMIDHSKKLAEKIRIS
                     GGGNCNFTNLDVGPDNFISDNPSFARSALARYKPQDFISLLYKHNIAFHEKTLGQLFC
                     DDGSEAIVAMLRGECDEAGVKRFMGCEVEEIKYTAPVDDDGVHLKRKFHVSTSRGEFT
                     AASLVIATGGLSIPKAGATPFGYKVAEQFGIPIVKLRPGLVPLSVAPDDWKAYADLTG
                     VSLDVVVSFGKRSFRENLLVTHRGLSGPAILQISSYWQHGEPLHIDLLPDLDMAAMLD
                     EQKTSKKHLSNYLTQWFSKGFADAWCGQLAQEQKIESKPLNQYNDKQRKQIAAALHDW
                     QVTPSGTLGYVKAEVTCGGVDTRALSSKTMECTEVPGLYFIGEVVDVTGQLGGYNFQW
                     AWASGYAAGQAV"
     gene            6002..6688
                     /locus_tag="VFW59_08295"
     CDS             6002..6688
                     /locus_tag="VFW59_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEX5364257.1"
                     /translation="MALPLFHGLNPEEIARLRELAGKLLADKAFSGAGGAEPDVAMAT
                     VIAAFAALPVLNLGYGWYEGWQEIVVYPGEFLHEGEQMDEAGVVHHVRHASSGESWEG
                     GPMVLSWQDVEQSGLGEGYNVVIHEFAHKLDMRNGSANGRPSLHSGMNPDDWAHDFLA
                     AYDDLCRRVDGGEEILIDPYAAESPAEFFAVLSECFFEAPDVLHAAYPEVYRQLQRFY
                     LQDPLMRLPA"
     gene            6739..7356
                     /locus_tag="VFW59_08300"
     CDS             6739..7356
                     /locus_tag="VFW59_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5364258.1"
                     /translation="MRALWMILLASLLSGCGYNTFQTTDEQIKASWAEVLNQYQRRAD
                     LIPNLVNTVKGYAAQEQAVLLGVTEARAKVGSIQATPELVNDPQAFANFQAAQGQLTS
                     ALSRLLVVSENYPQLKSDANFRDLQAQLEGTENRITVARKRYIEAVQEYNIAVRSFPE
                     NLTAMVFGYRVKPSFTVENEKAISAPPTVDFAAPPPAIPPAAPAK"
     gene            7443..8303
                     /locus_tag="VFW59_08305"
     CDS             7443..8303
                     /locus_tag="VFW59_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEX5364259.1"
                     /translation="MARKFNFSGCLFAITVLLALLTGAARAEVPQAEVAVPPLSARVT
                     DLTATLDAQQIQSLESRLAAFEAKKGAQLAVLIVPTTQPETIEQYGIRVAEAWKLGRK
                     GVDDGALLLVAKEDRAMRIEVGYGLEGALNDATAKRIVAEVITPFFKRGEFYAGIDAG
                     LDAMMKVVEGEPLPELQRRAPVGSQNMESLLFIAFGAVVVVGGMLRALLGRFPAALLM
                     GGGLGLLAWLTVAPLLIALLVGVMAFVFVLLGGGRGFGGFGGGGFGHGGGGFSGGGGG
                     FGGGGASGRW"
     gene            8541..9038
                     /locus_tag="VFW59_08310"
     CDS             8541..9038
                     /locus_tag="VFW59_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX5364260.1"
                     /translation="MNLKRIMRHLSSGRARVNRSFPPRALEAIERAIAVTETLHDGQI
                     RFAVEAGLDWQPLLAGQTARERAVEVFSQLRVWDTEHNNGVLIYLLLADRDVEIVADR
                     GIHARLGDEVWEAICSEMETAFREGRFEAGALAGIRAVGDHLNRHFPPRNGKPNEMPD
                     SAVLL"
     gene            9131..>9647
                     /locus_tag="VFW59_08315"
     CDS             9131..>9647
                     /locus_tag="VFW59_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5364261.1"
                     /translation="MDTPDRNEWQPQAGDALLIVDVQTDFLPGGSLAVPYGDEVLPVL
                     IRYLRAFSGLPVYATRDWHPERHCSFLAQGGDWPPHCIAGTQGAAFALQLPPAATIIS
                     KAATAEQEAYSGFQGTDLDERLRADNIRRLFVGGLATDYCVLATVRDALRLGYRVMLL
                     HDAIRAVDVRPG"
BASE COUNT         1786 a         2734 c         3268 g         1859 t
ORIGIN      
        1 gcgcaggggc ttacccctga cggtaccgtc ggatatcaga ccatggtgcg cctgagtgcg
       61 gtcgcggatc gagccgcgcc gagattattc cccgagcagg gagccaaata gccatgtcgt
      121 atattctgga tgcgctcaag aaatccgatc agcaacggca gcgcggtatg acgccgacgc
      181 tgccggaagc gcagccgaca ttggcagccc ccaggcgtac tgtgttgggg gcctatggcg
      241 tgttcgcggt ggtgttgctg ggcgcaggta ttgcaatcgg ctggctgcac ccctggcagg
      301 cagaacgagt cgctcctgcg gacgaggtcc tcgcggtgag gtcggctatc acggtgccgc
      361 gcccagccat ccttgcccct gtctcttctg aagctaacgg caataccgca cgggaatggt
      421 tggcgcagaa ctccacgccc ggcgtgccgt cctcccgcaa ggcagatatc ccggttcctc
      481 cggccgactc acctcgggag caaccggtga tatccatggc cgagttgccg cttgccatcc
      541 ggcaagagat tccggccatg acgattcagc tccacgctta ttcaagccag cccaagaacc
      601 ggctggtcag catcaactcg cggatgctgc atgaaggtga attgttggtg caggacttga
      661 agctggagca gatcacaccg gatggcatga tattcagtta caaggggtat cgtttccagc
      721 gcgggatacg ctagcaaggg ttgtgaaatt ttggattaac ttaaggaaac gcagattaag
      781 tcgttatttt ctaatcttgg gtctaattga aaaagagccg aacttgcaac ctgttgaatt
      841 ggttggcaaa tacggctctt tagtatgctg gcggagaagg ggggattcga acccccgttg
      901 ggatattatc ccaaacacgc tttccaggcg tgcgacttaa accactcatc cacctctcca
      961 gatggtactg cttgtatctt cttttggcat gcacaggcgg ttgcggcctg tgtcgagtcg
     1021 acttgttcag tcaactatcc gaagggcgcg cagaatatat cagaatcggt ttcgctgcaa
     1081 tcaaaactcg ttcttccatt ggcgcagggc ggtgaaagcc ccgagttcgc tggtatcggt
     1141 gatgccttgc gcttgcaggg tatggagaat ttccggggca ttgcagcgca ggaaggggtt
     1201 ggtggctttt tccagtgcga tggtggtggg gatgctcggc aggccggcat cgcgcagttg
     1261 ccgggtcgtg gcgatgcgtt gcaggagctc cgggttgccg ggttcgcagg ccagcgcgaa
     1321 gcgcaggttg gcttcggtgt attcgtgggc gcagtagacg cgggtgtcgt ccggcagttg
     1381 cgccagccgt tgcagcgagt ggtgcagctg cgccagcgtg ccttcgaagt tcctgccgca
     1441 gcctgcaccg aacagcacat cgccgcaaaa cagcatgccg ggagcgagat aggcgatgtg
     1501 gtcgtccaga tggccgggga ggtcgaggat gtcgaaggtg gcggctagcg tgtccagacg
     1561 cagtcgttcg ccttcgcgca ggtcgtgggt gatgtgcgga ttgtatgggt gtcgtcgtcc
     1621 atacaccggc acgttgtata cttcgcgcaa ttttgcgatg ccgccgatat ggtcatggtg
     1681 gcggtgggtg cacagaatcg cgtccagctt cagtccctgt gcggccaaaa aatccagcgc
     1741 cggtgcggcc tcgccgggat cgaccagtgc ggcatggctg tcgttgtgca gggcccagat
     1801 gtagttgtcc cgcagcgcgg gaatggcggt gatggttaac atggagcgga cgggaaatta
     1861 ggcgatgccg gattgtaagt taactgcaca gagtttgagc gagtggtttg ccagcgagca
     1921 gggcgcctat gtgctggccc gtgaacaggc gtatttcgac cgcacggtgg ccgatatctt
     1981 cggattcaat gcgctgcagc tgggcctgcc ggagcacggc tttctccgcg acagccggat
     2041 gccgctgcgc ttttctgcgg ggaatcaggc cggcaacgat gtgcgcctgt gttgtgcgga
     2101 gctgtcgttc gccacggcca gtctggatct ggtgctgttg ccgcatgtgc tcgagttttc
     2161 cgatcacccg caccagatcc tgcgcgaggt cgagcgggtg ttgatgccgg agggcaatct
     2221 gatcatcagc ggtttcaatc cgcgcagtct gtggggcttg catcgtgccg tgggtcggcg
     2281 gcagggttat ccgtggtgcg gacatttcat cgccttgtcg cgccttaagg attggctagc
     2341 cttgctggga ttcgaggtgg tgggcgggcg ctttgccgcc tatgcccctc cggtttatca
     2401 ggccaagtgg ctggagcgct tcgccttcat ggagcgggcg ggtgaccgct ggtgggcggt
     2461 gagcggcggg gtgtacttcc tgcatgcggt gaaacgggtg catggcatgc ggctgatcaa
     2521 gccgcagtgg aacgaggggc tggtgagcaa gctgatgcct gccgcgccga aattgaacaa
     2581 cagaatcacg caacatacgg gcatcgatcc ccgtgcgcat aaagaacatt tggaaaatta
     2641 gcaggtatgg ataaagagat cgtggacatt tttactgacg gcgcgtgcaa ggggaatccc
     2701 ggcgtcggcg gctggggcgc gttgttgcgc gtcaagggca aggaacgaga actgttcggc
     2761 ggcgaggcgc ataccaccaa caatcgcatg gagttgctgg gggcgatctc ggcactggag
     2821 gcgttgaagc ggcattgcaa ggtcaggctg cataccgatt cgaaatacgt gcagcagggt
     2881 atcagcacct ggatacatgg ttggaagctg cgcggctgga agacggcgga caggaagccg
     2941 gtgaagaacg aagacctgtg gcgcaggctg gatgctgtgg cggcgcaaca tcaaatcgag
     3001 tgggtgtggg tcaagggcca tgccgggcac gacggcaacg agcgcgccga cgaactggcg
     3061 aatcgcgggg tactttctgc agccgagaac cgtttggccg ggcacccgca gggaggtgag
     3121 caatgcgcag gatagtgctg gataccgaaa ccaccggact gaatccccag caggggcatc
     3181 gcatcatcga ggtggcggcc atcgtgctgg aggggcgccg tgtgtcgaag cgccactttc
     3241 atcattatgt cgatcccgag cgcgaggtcg atgaggaggc ggcccgcgtg catggcttca
     3301 cctgggacat gttgcgcggc cgggcgaagt tcgccgatat cgcagccggt ttactggagt
     3361 tcatcgggga ctccgagctg atcatccaca acgcgccgtt cgatatgggg ttcctcaata
     3421 acgaactggc gttgctcggt ctgccgccgt tgcgcaatcc ggtgctggac acgctcaagc
     3481 tggcgcgcga catgcatccg ggcaagaaga acaatctgga tgccctgtgc agtcgctacg
     3541 agatcgacaa cgcgcatcgc agcctgcacg gcgcgctgct ggataccgag ttgctggccg
     3601 aggtgtacat cgcgatgacg cgcgggcagg agagcctgct gggcgacgat tcgacgccgt
     3661 ccgaacgtca ggcggcactg caggaactgg atggtgagcg tcccaagttg cgcgtgttgc
     3721 agccgaccga agaggagctg gccgcccatg tgcagcaact ggccgacatc gacaaggcca
     3781 gcaagggcgc ctgtctgtgg caacagctcg aaggcgcggc atgagtcgtt ggcgccgtat
     3841 cgcggcaggt gttgtagtgg tatcgatggc ggggtatctc gccgcttgcg gttatatgtg
     3901 gtcgcagcag aaacatctgg tgttcgaacc cagcccggtg atacagacca cgcccgcgcg
     3961 tctggggatg aagttcgagg cgctgcgcat tccggtggga agcggtgatg agcgcgggga
     4021 gctggcggcc tggtgggttc cggcggagcg agcaggggcg gcgaccatac tgtattttca
     4081 cggcaactat cgcaacatcg gcaacaacct ggatcacacg cggcgcctgc acgacctcgg
     4141 ctataacgtg ctgctggcgg actatcgcgg tttcggcgag agcacgggcg ggcagcccag
     4201 cgaagcccgc gtgtacgagg atgccgaggc ggcctggcaa tatctgctga aggtgcgcgg
     4261 catcgatccg cgacaggcgt tcatctacgg ccattcgctg ggcggtgcgg tcgcgatcga
     4321 gctggctgtg catcaccccg aggcggctgg gctgatcacc gaaagcacct tcacctcgat
     4381 gcaggcgatg ggcgagctgg aatatggcta tctgcccgtc ggcctgatac tgaatcagcg
     4441 cttcgattcg ctgcagaaga tcgcagcgct gaaactgccg gtgctgctga tccacggcag
     4501 ctgggacaag aagataccgg cggcgatggc gcagcagctg tatgccgccg caccgcaacc
     4561 caagaggctg accctgatcg agggcggcga acacaacgac agcgggatgg tcggctgggt
     4621 cgaataccgc gatgcggtct cggcttttgt cggacaatac gcccattaac cagttaagcg
     4681 cccatcgagg gcgcaggaac cagatatgaa aatagatgtg attattatcg gcgcaggtgc
     4741 cgccggcctg atgtgtgcga tgcaagccgc gcaacgcggg cgtgccgtgg tgatgatcga
     4801 ccactccaag aaactggcgg agaagattcg catctcgggc ggcggcaact gcaacttcac
     4861 caacctcgat gtcgggccgg ataatttcat ttccgacaac cccagcttcg cgcgttccgc
     4921 gttggcgcgt tacaagccgc aggacttcat ctcgctgctg tacaagcaca acatcgcctt
     4981 ccacgagaag acgctgggac aattgttctg cgatgacggt tcggaagcca tcgtcgcgat
     5041 gttgcgcggc gaatgcgacg aggccggggt gaaacgcttc atgggttgcg aggtcgagga
     5101 gatcaaatat actgccccgg tcgatgacga tggcgtgcat ttgaagcgca aattccatgt
     5161 cagcacctcg cgcggcgaat tcacggcagc ctcgctggtc atcgcgaccg gcggtctgtc
     5221 catccccaag gccggcgcga cgccgttcgg ctacaaggtg gccgaacagt tcggcattcc
     5281 catcgtcaag ctgcgccccg gtctggtgcc cttgagtgtc gcaccggacg actggaaggc
     5341 ctatgccgac ctgaccggcg tgtcgctcga cgtggtggtg agtttcggca agcgctcgtt
     5401 ccgcgagaac ctgctggtca cccatcgcgg cctgagcggc ccggcgatcc tgcaaatctc
     5461 ctcctactgg cagcacggcg aaccgctgca tatcgacctg ttgcccgacc ttgacatggc
     5521 ggcgatgctg gacgagcaga agaccagcaa gaagcacctg agcaattacc tgacgcaatg
     5581 gttctccaag ggcttcgccg atgcatggtg cgggcaactg gcgcaggaac agaagatcga
     5641 gagcaagccg ctgaaccagt acaacgacaa gcagcgcaag cagatcgcgg ctgcgctgca
     5701 cgactggcag gtcaccccga gcggcacgct gggttacgtc aaggccgagg tcacctgcgg
     5761 cggcgtggat acccgcgcgc tgtcgtccaa gaccatggaa tgcaccgagg tgcccggact
     5821 gtatttcatc ggcgaggtag tggacgtgac cggccagctc ggcggctaca acttccagtg
     5881 ggcctgggcg tcgggctacg cggcggggca ggcggtatag caggagatgg ggttcctttc
     5941 cgggctgttc ggctggggga acgctccgct tgtagcggac gatggcgtct ggcaaaggac
     6001 catggcactg ccgctgttcc acggactgaa tcctgaggaa atcgcccgcc ttcgggagct
     6061 ggcaggcaag ctgctggccg acaaggcgtt ctcgggggct ggcggggcgg agccggatgt
     6121 cgccatggca accgtcatcg ccgccttcgc ggccctgccg gtgctgaacc tgggctacgg
     6181 ctggtacgaa ggctggcagg agatcgtggt ctacccgggc gaatttctcc atgagggcga
     6241 acagatggac gaggccggcg tcgtccacca tgtccgccat gcgagcagcg gcgagtcctg
     6301 ggagggcggc ccgatggtgt tgtcctggca ggacgtcgag cagtcgggat tgggcgaggg
     6361 ctacaacgtc gtgatccatg aatttgccca caagctggac atgcgcaacg gcagcgcgaa
     6421 tggccggccg tcgctgcact ccgggatgaa cccggatgac tgggcgcacg acttcctggc
     6481 cgcctacgac gacctctgtc gccgggtgga cggcggcgag gagatcctga tcgatcccta
     6541 tgcggcggag agcccggcgg aattcttcgc ggtgctgtcc gaatgcttct tcgaggcgcc
     6601 cgatgtgctg cacgccgcct atcctgaggt gtaccggcaa ctgcagcgct tctatctgca
     6661 ggatccgttg atgcggctgc cggcctagat actttagtgc tatagttttc ccgctattcc
     6721 aaccttgagg agtcgacgat gcgtgcgcta tggatgattt tgctggcttc gctgttgagc
     6781 ggttgcggct acaacacttt tcaaacgaca gacgagcaga tcaaggcgag ctgggcggag
     6841 gtgctgaacc agtaccagcg ccgcgccgat ctgattccca atctggtgaa cacggtcaag
     6901 ggctatgccg ctcaggagca ggcggtgctg ctgggcgtca ccgaggcgcg cgcgaaggtc
     6961 ggcagcatcc aggccacacc cgagctggtc aacgatcctc aggccttcgc caacttccag
     7021 gccgcgcagg ggcaactgac ttcggcgctg agccgattgc tggtggtgtc ggagaactat
     7081 ccgcaactga agtcggatgc gaacttccgc gacttgcagg cgcagctgga aggcaccgag
     7141 aaccgcatca ccgtggcgcg caagcgttac atcgaggcgg ttcaggaata caacatcgcg
     7201 gtgcgttcct tccccgaaaa cctgactgcg atggtgttcg gttaccgcgt caaaccttcg
     7261 ttcacggtcg agaacgagaa ggcgatctcg gctccgccga ccgtggactt cgccgctccg
     7321 ccccctgcga taccccctgc cgcaccggca aaatgaacac tctggcgcga gtcgttaacc
     7381 tgacactcgc gaagacctta gtccccctct cccgcttgcg ggagagggga ggggagacgg
     7441 ttatggcacg aaaatttaat ttttctggat gtctctttgc cataaccgtt ctgcttgcgt
     7501 tgctgaccgg cgcggcgcgg gccgaggtgc ctcaggctga agtagcggtt ccgccgcttt
     7561 ccgcgcgcgt gacagatctc actgccacgc tggatgcgca acagatacag tcgctggaat
     7621 cccgcctcgc cgcattcgag gcgaagaagg gcgcacagct cgcggtgctg atcgtgccga
     7681 ccacccagcc tgaaaccatc gagcaatacg gaatccgcgt cgccgaagca tggaagctcg
     7741 ggcgcaaggg tgtggacgac ggcgcgctgc tgctggtcgc gaaggaggac cgcgccatgc
     7801 gcatcgaggt cggctacggg ctggaaggcg cgctgaacga cgccaccgcc aagcgcatcg
     7861 tcgccgaggt catcacgccg ttcttcaaac gcggcgaatt ctatgcaggt atcgatgcgg
     7921 gtctcgacgc catgatgaaa gtggtcgagg gcgaaccttt gccggaattg cagcgcaggg
     7981 ctcccgtcgg cagtcaaaat atggagtcgc tgctgttcat cgcgttcggc gcagtggtgg
     8041 tcgtgggcgg catgctgcgc gcgctgcttg ggcgtttccc ggctgcgctg ctgatgggag
     8101 gcgggctcgg cttgctggcc tggctgacgg tcgccccgtt gctgatcgcg ctgctggtcg
     8161 gcgtgatggc cttcgtgttc gtgctgctgg gcggcggacg cggattcggc ggttttggcg
     8221 gaggcgggtt cggccatgga ggcggcggtt tttccggtgg cggaggcggc tttggcggag
     8281 gcggtgcttc ggggagatgg tgagcgcgca tcccgcaaag ctactgcgcg acttgatttc
     8341 ggcgttgcgc ggtgttggtt ccagcatcac gcgctttgcg catgagccta caccgcaact
     8401 tcgttgtcga aatccggggc cgcgtagcgc gcagactgaa agtacattcc ggtttctgcg
     8461 ttccgtgcgc cttaaactca agccgctcgc tacgattttc ggaatgcgcg aaatggcaat
     8521 actgtgatga gagatattga atgaacttga aacgcatcat gcggcatctg tcgagcgggc
     8581 gcgcgagggt caaccgcagt tttccgcccc gtgcgctcga ggcgatcgaa cgcgcgatcg
     8641 ccgtcaccga aacattgcat gacggacaga tacgcttcgc ggtcgaggcc ggactggact
     8701 ggcagccttt gctggccggg cagacggccc gcgagcgcgc cgtcgaggtg ttctcgcaat
     8761 tgcgcgtctg ggacaccgag cacaacaacg gcgtgctgat ctacctgctg ctcgccgacc
     8821 gcgacgtgga gatcgtcgcc gaccggggca tccatgccag actcggcgat gaggtctggg
     8881 aggcgatctg cagcgagatg gagacggcgt tccgcgaagg tcgtttcgag gccggcgcgc
     8941 ttgcggggat acgcgcggtc ggggatcatc tcaatcgcca ttttccgccg cgcaatggca
     9001 agccgaacga gatgccggac agtgcggtgt tgctgtagcg actgcccgga tgaacgcagc
     9061 agaaggctgc atatgcaccg caaggtggtg ccgttgatct gtacaagggt tagcaagagc
     9121 gaggtgagcc atggacacac cggatcggaa tgaatggcag ccgcaggcgg gtgacgcact
     9181 gctgatcgtc gatgtgcaga ccgattttct gcccggaggc agccttgcgg tgccgtatgg
     9241 cgacgaggtg ttgccggtgc tgatccgcta cctgcgcgcc ttttccgggc tgccggtgta
     9301 tgcgacgcgg gactggcatc ccgagcgcca ttgctcgttt ctggcacagg gcggcgactg
     9361 gccgccgcat tgcatcgccg ggacgcaggg cgcggcattc gcgctgcaac tgccacctgc
     9421 tgcgacgatc atctcgaagg ctgccacggc agagcaggag gcctattccg gatttcaggg
     9481 caccgacctc gatgagcggc tgcgcgccga caacatccgc cgcctgtttg tcggcgggct
     9541 tgcgaccgat tactgcgtgc ttgccaccgt gcgcgatgcg ctgaggctgg gctaccgggt
     9601 gatgctgctg catgacgcga tccgtgcggt ggatgtccgg cccggcg
//
LOCUS       DASUIV010000228         8253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_708319, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000228 DASUIV010000000
VERSION     DASUIV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708319"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VFW59_08320"
     CDS             <1..846
                     /locus_tag="VFW59_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX5364262.1"
                     /translation="AVGPRLNAAGRLDDMSAGIAGLLIDDEAAATQIAAELDALNRER
                     RHIEADMQDSALAALETIDPQDGYSLTLFDAGWHQGVIGILASRLKDKFHRPVLAFAR
                     ANNGEIKGSGRSIPGLHLRDALDLVSKRHPALIKKFGGHAAAAGLSIAEADFKTFVAA
                     FEQVSGELLDEGDLVRRIETDGALDPGEMNLDIARLLGQQVWGQGFPAPQFDDAFIVK
                     SQRVVGEKHLRLKLEKQGQLVDGILFNQAEPLPDRVRAVYSLDINEYKGSQSLQLIVR
                     HWQAE"
     gene            857..2119
                     /locus_tag="VFW59_08325"
     CDS             857..2119
                     /locus_tag="VFW59_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5364263.1"
                     /translation="MNLDLLQGNGVAALPQFLTSLAIGLLIGMERERNPSAKAGLRTF
                     ALVAVFGTLCALLSTKLGTPWILLTGLVVVAAMIIAAYFNCPTEENDPGTTTVIALIL
                     SYCLGAMIWYELTRLAVMLAIGITVLLYFKPELRGLSQRLTRRDLVAVLQFAVLTFII
                     LPILPDQSYGPYQAFNPHQAWLMVVLISGLSLTGYAALHIVGSRYGAPLLGFFGGLVS
                     STATTMIYAKHGKSDPTMEKLATSVIVIASLVVLIRLLLVSSVVAYATLSALAPVLVG
                     GLICGLIVVAYLWRKTDVNQELYIPETANPAELHTAIGFGLLYVLVLVGAAWMLDVAG
                     SQGLYIVALVSGLTDVDAITLSSLRLFNVGQLNEVQTVTAIAIAFISNLTFKLGMVMF
                     IGGKQLAKRVAVGFAAMAFGVLAGLIAL"
     gene            2149..3253
                     /gene="prfB"
                     /locus_tag="VFW59_08330"
     CDS             join(2149..2229,2231..3253)
                     /gene="prfB"
                     /locus_tag="VFW59_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293703.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5364264.1"
                     /translation="MEAEQLNAISNQLQDLALRSAELRRYLDFDAKQERLVEVCELSE
                     DPAIWEDAKRAQELGRERKSLEAVVLGLTSIKQGLTDAGELFEMAREENDEESLLSIA
                     SDIKDIEKRVAELEFRRMFSHPMDPNNCFIDIQAGSGGTEAQDWGSMLLRMYLRYCER
                     KGFQVEILEQSDGEVAGIKGASIKVIGDYAYGHLRTETGVHRLVRKSPFDSGNRRHTS
                     FSSVFVYPEVDDSIEIDINPADLRVDTYRASGAGGQHINKTDSAVRLTHIPTGIVVQC
                     QNDRSQHKNRAEAMSMLKSRMYEEELRKRNAEKQALEDTKSDIGWGHQIRSYVLDQSR
                     IKDLRTNHEVGNTQGVLDGDLDDFISASLKQGV"
     gene            3281..4852
                     /gene="lysS"
                     /locus_tag="VFW59_08335"
     CDS             3281..4852
                     /gene="lysS"
                     /locus_tag="VFW59_08335"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5364265.1"
                     /translation="MTTEPTDIHQDDNAVATTANIGEADVKSHSDGESQNQIITERRN
                     KLAEIRKAGVAFPNDFDRTHLANNLHVDFNDQTPEQLEAAQINVSVAGRMMLKRVMGK
                     ASFASVQDMTGRIQLYISNGDTGEEAHNAFKHYDLGDILGASGVLFKTKTGELSVRVT
                     QVRLLTKSLRPLPEKFHGLSDQEQKYRQRYLDLITNEDARNVFKKRTQIVQAIREFFI
                     RHDYLEVETPMLHSIPGGASAKPFVTHHNALDMQMFLRIAPELYLKRLVVGGFEKVFE
                     INRNFRNEGLSTRHNPEFTMLEFYEAYRDYKYLMDFTERLFREVAEKVLGTTQISYQG
                     KELDLGKPFHRLTMVQAIQKFHPQFTDAQLNDRSFVVNELEDLKAKYKPEDGIGGLQL
                     SLFEELAEAHLFEPTFIIDYPVEVSPLARSSDKNHDITERFELFIVGREIANGFSELN
                     DAEDQEARFDAQVAAKDAGDEEAMFKDNDYVRALEYGLPPTAGQGIGIDRLVMLLTDS
                     ASIRDVILFPHMRPE"
     gene            4865..5530
                     /locus_tag="VFW59_08340"
     CDS             4865..5530
                     /locus_tag="VFW59_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5364266.1"
                     /translation="MLNSDTKALLLQHYPMLDGLPVAELDALLAGTNVMQLPAGTVIF
                     DEDQPCQGFPLLLAGNVRVIKSSPNGRELQLYRVGAGESCILTSSCLLGHTPYHARGL
                     VESDVRMVVLSPATFKKLVSTQEAFRNYIFALFSERLTDLMQLVSAVAFQKLDQRLAA
                     LLAGKPSPILTTHQALADELGSVREIVSRLLKNFAEQGWIRLGREQIEIVDIPALRKF
                     STL"
     gene            5616..6080
                     /locus_tag="VFW59_08345"
     CDS             5616..6080
                     /locus_tag="VFW59_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364267.1"
                     /translation="MRLNKLALFAIALWVATIATFVWFFVRGNTTEGTGNRTAVVLQA
                     GERELVLSEMRGLLGATQGILQGINSNDMKQVAAAARSGGMASAADVNPALMAKLPLP
                     FKQLGMSVHHSMDDLAVAAESGKPSAELIGMLKNTLSSCVACHAAWQLQAAK"
     gene            6125..6319
                     /locus_tag="VFW59_08350"
     CDS             6125..6319
                     /locus_tag="VFW59_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX5364268.1"
                     /translation="MKLNVGGIDRIARIIAGLVLIGLAAANMIGVWGYIGLVPLLTGI
                     FKFCPAYAIFGMNTCPMNKD"
     gene            6383..6955
                     /locus_tag="VFW59_08355"
     CDS             6383..6955
                     /locus_tag="VFW59_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEX5364269.1"
                     /translation="MKKRFAFASLLVLSSASALADDLARQREESRVIIGPFIQQLVAE
                     NKKAVMEGGPESAIGVCRDIAPKMSGEISRQNGLRLTRVSLKVRNPLLGTPDIWEQKT
                     LKQFEARLAKGEKPEMLEAAEIVSEPAGKYFRYMKGIVLQPGCVACHGTNEQISDNVK
                     AILATEYPHDQATGYAPGQLRGGVSLKRPM"
     gene            6995..>8253
                     /locus_tag="VFW59_08360"
     CDS             6995..>8253
                     /locus_tag="VFW59_08360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5364270.1"
                     /translation="METKSDHAELQITGMRCASCSARLEKNLNQLPDVKAVVNIATEK
                     ASLTFDPTVTDVATLIEAVRKAGFDAHPVRDFAAEKQARADELRRDRNLFFISMGLTL
                     PLLGEMLLMPLGIHLMLPVWLQWLLASPVQFWTGARFYRGAWSAVRSGGSNMDVLVSL
                     GTSAAYFMSVAVWLLGLDQPVYFEASATLITLVLLGKLLEARAKGKASAALEALIKLQ
                     PKIAHVERNGQLLDVSAGSLRPDDIFIVRPGESMPVDGMVLEGSSSVDEAMLTGESLP
                     QSKQPGAKVYAATLNQQGLLRCRALAVGSHTQLAAIIRLVEQAQGSKAAIQKLADQIS
                     AVFVPTVLGIALLTFAGWWLANAGFTAALINAVSVLVIACPCALGLATPTAVVVATGR
                     AAHAGILVKDAAALERAHKLAVLVVDKT"
BASE COUNT         1711 a         2683 c         2409 g         1450 t
ORIGIN      
        1 gccgtcggcc cgcgcctgaa tgcggcggga cgactggacg acatgagcgc gggcatcgcc
       61 ggactgctga tcgacgacga agccgccgcg acacagatcg ccgccgagct cgacgcgctg
      121 aaccgcgaac ggcgccacat cgaagccgac atgcaggaca gcgcgctggc cgcgctggaa
      181 accatcgacc cgcaggacgg atacagcctg acgctgttcg acgcaggctg gcatcagggc
      241 gtgatcggta tcctcgcctc tcgcctcaag gacaaattcc accgtcccgt gctcgccttc
      301 gcccgcgcca acaacggcga gatcaagggc tccggacgct ccatccccgg cctgcacctg
      361 cgcgatgcac tggatctggt cagcaaacgt catcccgcac tgatcaagaa gttcggcggt
      421 catgcggcgg cggcgggact gagcatcgcc gaggccgact ttaaaacctt cgtcgctgcc
      481 ttcgaacagg tttccggcga actgctggac gagggcgatc tggtcaggcg catcgagacc
      541 gatggcgcgc tggatcccgg cgaaatgaat ctggacatcg cccgcctgct cggccaacag
      601 gtgtggggcc aaggcttccc ggccccgcag ttcgacgacg ccttcatcgt caagagccag
      661 cgcgtggtcg gcgagaagca cctccggctc aagctggaga aacaggggca gctcgttgac
      721 ggcatcctgt tcaatcaggc cgagcccctg ccggatcgcg tgcgcgccgt gtatagtctg
      781 gacatcaacg aatacaaagg cagccagagc ctgcaactga tcgttcggca ctggcaggca
      841 gaataaggat gtgacgatga acctcgatct cctgcaggga aacggcgttg cggccctgcc
      901 gcaattcctt accagcctcg ccatcggcct gctgatcggg atggagcgcg aacgcaatcc
      961 ctcggccaag gccgggttgc gcaccttcgc gctggtcgcc gtgttcggca cgctgtgcgc
     1021 cctgctctcc accaaactcg gcaccccctg gatactgctg accggtctgg tggtcgtggc
     1081 tgcgatgatc atcgccgcct atttcaattg cccgaccgag gaaaacgatc caggcaccac
     1141 cacggtgatc gcactgatac tcagttattg tctgggcgcg atgatctggt acgaactgac
     1201 cagactcgcc gtgatgctgg cgatcggcat caccgttctg ctctatttca aacccgagtt
     1261 gcgcggcctg tcgcaacgcc tgacgcgccg cgatctggtc gccgtgctgc aattcgccgt
     1321 gctcaccttc atcatcctgc ccatcctgcc ggatcagagc tatgggcctt atcaagcctt
     1381 taacccgcat caagcctggc tgatggtggt actcatctcc gggctcagcc tgacgggcta
     1441 tgcggcgctg cacatcgtcg gcagccgcta cggtgcgccc ttgctcggct tcttcggcgg
     1501 actggtttcc agcaccgcca ccaccatgat ctacgccaag cacggcaaga gcgacccaac
     1561 catggaaaag ctcgccacct cggtaatcgt catcgccagc ttggtggtac tgatacgcct
     1621 gcttctggtc agctccgtgg tcgcctacgc cacgctgtcc gcactggcac cggtgctggt
     1681 gggcgggttg atatgcggcc tgatcgtcgt cgcctacctc tggcgcaaga ctgacgtgaa
     1741 tcaagagctc tacatccccg aaaccgccaa cccggccgag ctgcacaccg ccatcggatt
     1801 cgggcttttg tacgtactcg tactggtcgg cgcagcctgg atgctggacg ttgcgggcag
     1861 tcagggtctg tacatcgttg cgctggtatc gggcttgacc gatgtcgatg ccatcacgct
     1921 ttccagcctg cggctattca acgtcggcca gctgaacgaa gtgcaaaccg tcacggcaat
     1981 cgccatcgcc ttcatctcca atctcacctt caaactcggc atggtgatgt tcatcggcgg
     2041 aaaacaactc gccaaacggg tcgccgtcgg cttcgcggcg atggcgttcg gcgttctggc
     2101 cgggctgatt gcgttataat cgcgcccttt tcagcacgcg acgacatcat ggaagccgaa
     2161 caactcaacg ccatctccaa tcagctgcaa gaccttgctc tgcgtagcgc cgaattacgg
     2221 aggtatcttt gacttcgatg ccaagcaaga acgcttagtc gaagtctgcg aactctccga
     2281 agacccggcc atctgggaag acgccaagcg cgcccaggag ctgggccgcg aacgcaagtc
     2341 gctggaagcc gtcgtgctcg gcctgaccag catcaagcaa ggcctcaccg atgccggcga
     2401 actgttcgag atggcgcgcg aggagaatga cgaagagagc ctgctcagca tcgcctccga
     2461 catcaaggac atcgagaagc gcgtcgcgga actggaattc cgccgcatgt tctcccaccc
     2521 gatggatccg aacaactgct tcatcgacat ccaggccggc tccggcggca ccgaggcgca
     2581 ggactggggc tcgatgctgc tgcgcatgta tctgcgctac tgcgaacgca agggcttcca
     2641 ggtcgagata ctggaacagt ccgacggcga agtcgctggc atcaagggcg catccatcaa
     2701 ggtgatcggc gactatgcct acggccatct gcgcaccgag accggcgtgc accgtctggt
     2761 gcgcaagtcg ccgttcgact ccggcaaccg ccgccatacc tcgttctcca gcgtgttcgt
     2821 ctaccccgaa gtggacgact ccatcgagat cgacatcaac cccgccgact tgcgcgtgga
     2881 cacctaccgc gcatccggcg cgggcggtca gcacatcaac aagaccgact cggcggtgcg
     2941 gctcacccac atcccgaccg gcatcgtggt gcagtgccag aacgaccgct cgcagcacaa
     3001 gaaccgcgcg gaggcgatgt ccatgctgaa gtcgcgcatg tacgaagaag agctgcgcaa
     3061 gcgcaacgcc gagaagcagg cgctggaaga caccaagtcc gacatcggct ggggccacca
     3121 gatccgctcc tacgtgctcg accagtcgcg catcaaagac ctgcgtacca accacgaggt
     3181 cggcaacacc cagggcgtgc tcgacggcga cctcgacgac ttcatctcgg ccagcctgaa
     3241 gcaaggcgtt taactgtaac catcattccc agagaacgac atgaccaccg aaccgacaga
     3301 catccatcag gacgacaacg cagttgcgac gaccgctaat atcggcgaag ccgatgttaa
     3361 gtcacatagt gacggcgaga gccaaaacca gatcatcacc gagcgccgca acaagcttgc
     3421 ggagatacgc aaagcgggcg tcgccttccc taacgacttc gatcgcacgc atctggcgaa
     3481 caacctgcat gtcgatttca acgaccagac gccagagcag ctggaagcgg cgcagattaa
     3541 tgtgtccgtg gccgggcgca tgatgctcaa gcgcgtgatg ggcaaggcca gtttcgcctc
     3601 ggtgcaggac atgactgggc gcatacagct gtacatcagc aacggcgaca ccggcgaaga
     3661 ggcgcacaac gcgttcaagc attacgacct cggcgacatc ctgggtgctt ccggcgtgct
     3721 gttcaagacc aagaccggcg agctgtcggt gcgcgttacg caggttcgcc tgctgaccaa
     3781 gtcgctgcgt ccgctgccgg aaaaattcca cggtctgagc gaccaggagc agaaataccg
     3841 ccagcgttat ctcgatctga tcaccaacga agacgcgcgc aacgtgttca agaagcgcac
     3901 gcagatcgtt caggcgatac gcgaattctt catccgccac gactacctgg aagtcgagac
     3961 gccgatgctg cattcgatcc ccggcggtgc ctcggccaag cctttcgtca cccaccacaa
     4021 tgcgctggac atgcagatgt tcctgcgcat cgcgcccgag ctgtatctga agcgtctggt
     4081 ggtgggcggc ttcgagaagg tgttcgagat caaccgcaac ttccgcaacg agggcctgtc
     4141 cacgcgccac aaccccgagt tcacgatgct ggaattttac gaggcctatc gcgactacaa
     4201 gtacctgatg gacttcaccg agcgcctgtt ccgcgaggtc gcagagaagg tgctgggcac
     4261 cacgcagatc agctatcagg gaaaagagct ggacctgggc aagcccttcc atcgtctgac
     4321 catggtgcag gcgatccaga aattccatcc gcagttcacc gacgcacagc tgaacgaccg
     4381 cagcttcgtg gtcaacgaac tggaagacct gaaggcgaaa tacaagccgg aagacggcat
     4441 tggcggcctg cagctttcgc tgttcgaaga gctggccgag gcgcatctgt tcgagccgac
     4501 cttcatcatc gactacccgg tggaagtctc gccgctggca cgcagcagcg acaagaacca
     4561 tgacatcacc gaacgcttcg agctgttcat cgtcggtcgc gagatcgcca acggcttctc
     4621 cgagctgaac gacgcggaag accaggaagc gcgcttcgac gcacaggtcg ccgccaagga
     4681 tgcgggcgac gaggaggcga tgttcaagga caacgactac gtgcgcgcgc tggaatacgg
     4741 cctgccaccg accgcaggac agggcatcgg catcgaccgt ctggtgatgc tgctgaccga
     4801 cagcgccagc atccgcgacg tgatcctgtt cccgcacatg cgcccggaat agcaagacca
     4861 tcccatgctg aacagcgaca ccaaagcgct cctgctccag cattacccga tgctcgacgg
     4921 actgcccgtc gccgaactgg acgcgctgct ggccggcaca aatgtgatgc agcttccggc
     4981 tggcacggtg atattcgacg aggatcagcc ctgccagggc ttcccgctgc tgcttgcggg
     5041 caatgtgcgc gtcatcaaga gttcgccgaa cgggcgcgag ctgcaactgt atcgcgtcgg
     5101 tgctggcgag agctgcatcc tcaccagcag ctgcctgctg gggcacactc cctatcacgc
     5161 gcgcgggctg gtggagagcg acgtgcgcat ggtggtactc tcccccgcga cattcaagaa
     5221 actggtctcc actcaggaag cgttccgcaa ttacatcttc gcgctgttct cggaacgcct
     5281 caccgacctg atgcagctgg tctcggcggt cgccttccag aagctggatc agcgcctcgc
     5341 cgcattgctg gcgggcaaac ccagcccgat actcaccacc catcaggcat tggccgatga
     5401 gctcggcagc gtgcgcgaga tcgtcagccg tctgctgaag aacttcgccg agcagggctg
     5461 gatcaggctg ggccgggagc agatcgaaat cgtcgatatc cccgcactgc gaaagttttc
     5521 gacactgtag ccctctgtga ctttcctcac agacagtcct ccctgccccg cctaatatcc
     5581 gtcaccgttc acattggaca ccaccggata accccatgag actcaacaag ctcgccctgt
     5641 tcgccatcgc cctgtgggtc gccaccattg cgaccttcgt ctggtttttc gtgcgcggca
     5701 ataccaccga aggcacagga aatcgcaccg cagtggtgct gcaggccgga gaacgcgagc
     5761 tggtgctcag cgaaatgcgc ggcctgctcg gcgccaccca gggcatcctg cagggcatca
     5821 acagcaacga catgaaacag gtcgccgcag cggcacgttc cggcggcatg gcttcggccg
     5881 ccgacgttaa ccccgcactc atggcaaaac tgcccttgcc cttcaaacaa ctgggaatga
     5941 gcgtgcacca cagcatggat gacctcgccg ttgcggcgga aagcggcaag ccctctgccg
     6001 aactcatcgg catgctgaaa aacacgctgt cgagctgtgt tgcctgccat gcggcgtggc
     6061 agctgcaggc agccaagtaa tcggataaat tttcaaccca ccttccaacc acaaggagat
     6121 catcatgaaa ctcaacgtag gcggtattga ccgcatcgca cgcatcatcg caggactggt
     6181 tctgattggc ctggccgccg ccaacatgat cggcgtatgg ggctatatcg gcctggtgcc
     6241 attgctgacc ggcatcttca aattctgccc ggcctatgcg attttcggca tgaacacctg
     6301 cccgatgaac aaggactaag caatctttat ccctgacgcc cgatgaagtt catcgggcgt
     6361 tttttacgca caggagatac ccatgaaaaa acgtttcgca tttgcatccc tgctggttct
     6421 ttcttccgcc tccgctctgg ccgacgacct cgccagacag cgtgaagaaa gccgcgtcat
     6481 catcggcccg ttcatccagc aactggtcgc agaaaacaaa aaggccgtga tggaaggcgg
     6541 cccggaatcc gccatcggcg tctgccgcga catcgccccg aagatgtccg gggaaatctc
     6601 gcgccagaac ggattgcgac tcactcgcgt ttcgctcaag gtacgcaacc cgctgctcgg
     6661 cacaccggac atctgggaac agaagaccct gaagcagttc gaggcacgtc tggccaaggg
     6721 tgagaagccg gagatgctgg aagccgccga gatcgtgagc gagcccgccg gcaagtattt
     6781 ccgctacatg aagggcatcg tgttgcagcc cggctgcgtc gcctgccacg gcacgaacga
     6841 acagatatcc gacaacgtga aggcgattct ggcaaccgag tatccgcacg atcaggcgac
     6901 cggctatgca cccggccaac tgcgcggcgg cgtgagcctg aagcgcccga tgtaagccaa
     6961 cgtccgctgt aaatcgtgct acattcggcg tgacatggaa accaaatccg atcacgccga
     7021 actgcaaatc accggaatgc gctgcgcatc ctgctccgcg cgtctggaaa aaaacctcaa
     7081 ccagctgccc gatgtaaaag ccgtggtgaa tatcgccacg gaaaaggcca gcctcacgtt
     7141 cgatccaacc gtcaccgacg tggcaaccct catcgaagcc gtacgcaaag ccggattcga
     7201 cgcccacccg gtgcgcgatt tcgccgccga aaaacaggcg cgcgcggacg aactgcggcg
     7261 cgaccgcaac ctgttcttca tctccatggg cttgaccctg ccgctgctgg gcgaaatgct
     7321 gctgatgccg ctcggcatcc acctgatgct gccggtctgg ctgcaatggc tgctcgcctc
     7381 gccggtgcaa ttctggaccg gtgcacgctt ctatcgcggc gcatggagtg ccgtcagaag
     7441 cggcggcagc aacatggatg tgctggtctc actcggcacc agcgcggcgt atttcatgag
     7501 cgtcgcggta tggctgctcg gtctcgacca gcctgtctac ttcgaagcga gtgcgacgct
     7561 gatcacgctg gtgctgctcg gcaagttgct ggaggcgcgc gcaaaaggaa aagcttccgc
     7621 cgcgctcgaa gcgctgatca agctgcaacc caaaatcgcg catgtcgaac gcaacggtca
     7681 gctgctggat gtctcggcag gttcgctgcg ccccgacgac attttcatcg tgcgccccgg
     7741 cgagagcatg ccggtcgatg gcatggtgct ggaaggctcc tccagcgtgg acgaagcgat
     7801 gctcaccggc gagagcctgc cgcaaagcaa gcaaccgggc gccaaggtgt acgcggcgac
     7861 gctgaaccag cagggcctgc tgcgctgccg cgcgctggca gtcggctcgc atacccagct
     7921 cgccgcgatc atccggctgg tggagcaggc gcagggctcc aaggccgcga tccagaaact
     7981 cgccgaccag atttccgccg tgtttgtacc cacggtgctg ggcatcgcgc tgctgacctt
     8041 cgccggctgg tggctggcga atgccggctt caccgcagcg ctcatcaacg cggtgtcggt
     8101 gctggtcatc gcctgcccct gcgcgctggg cttggccacc cccaccgccg tggtggtggc
     8161 aacaggccgc gccgcccatg ccggaatact ggtcaaggat gccgccgcac tggaacgtgc
     8221 gcacaagctc gcggtgctgg tggtggacaa gac
//
LOCUS       DASUIV010000229        15725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_150136, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000229 DASUIV010000000
VERSION     DASUIV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 15725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15725
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150136"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VFW59_08365"
     CDS             complement(<1..172)
                     /locus_tag="VFW59_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEX5364271.1"
                     /translation="MTQEISTEVLLEKYAEPGETSVEDVRRRVARGLAQAEKPEQRAH
                     WEEAFFQAQESGF"
     gene            complement(175..789)
                     /locus_tag="VFW59_08370"
     CDS             complement(175..789)
                     /locus_tag="VFW59_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364272.1"
                     /translation="MNNRIRQIAEQITALETELDAAIEEHEQRLLYQLEGRRVVFEQA
                     VRDAHRRVKLGVFRWFLTVRPQNYLTMPIIYGAIIPMTLLDLCLSLYQLICFPVYRIA
                     RVKRGDYIVFDHQHLAYLNIIEKAHCLYCSYAVGLLAYATEIIARTEQYFCPIKHARR
                     VRAAHARYKNFLDYGEADDYHGKLEEFRAELAQEADAKTATKKE"
     gene            955..1665
                     /locus_tag="VFW59_08375"
     CDS             955..1665
                     /locus_tag="VFW59_08375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5364273.1"
                     /translation="MNEAIPMRRFFILLGLIYLIEMLLMLTVEPVLISANYPFWIRAI
                     VDSALLAIICVPFMLNYQLRPMQMAYHDSLTGLPNRLLFHDRLEHALLAAKRNKSSCA
                     VVFLDLDRFKPVNDICGHRVGDVVLKQAASRIEGCMRESDTVARIGGDEFAILLSGPV
                     QKADADQVVQKISEALSLPFEVRGKRLDLGVSAGIAFYPQNGTDGMTLLDAADDAMYR
                     AKEIKKSSICDAMQGSRI"
     gene            complement(1662..2498)
                     /gene="serB"
                     /locus_tag="VFW59_08380"
     CDS             complement(1662..2498)
                     /gene="serB"
                     /locus_tag="VFW59_08380"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX5364274.1"
                     /translation="MNLILQALHDIPSAQVEHLAKLSNAARIEQIGPHAYRLREAKPH
                     DGIASYCLEQQIDCGFVAQGKHLSDFGLVVMDMDSTLISIECIDEIADMQGLKPQVSA
                     ITEEAMRGEIDFAESLRRRVALLQGLDEGALLRVYDERLQLNPGAPIMLAALKQHGIK
                     TLLVSGGFVFFTERLKARLGLDHAHANTLEIVDGKLTGRVLGAIVDAQSKADWLVKTR
                     DELGLKPEQVIAMGDGANDLKMMAQAGLSVAYHAKPVVREQASYALNFVGLDGLVNLL
                     DN"
     gene            complement(2498..5899)
                     /gene="mfd"
                     /locus_tag="VFW59_08385"
     CDS             complement(2498..5899)
                     /gene="mfd"
                     /locus_tag="VFW59_08385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5364275.1"
                     /translation="MLFTSKHSRPRYTRLHGSSDALALAQYAGTHSPLVVIAANALEA
                     QRLVEEIPFFDTKLRVHLLPDWETLPYDHFSPHQDLISERLATLHHIRSNACDVVIVP
                     ITTALYPLPPVEHLAAYTFFLKRGEKLDVAQLREQMTFAGYNHVQQVLTPGEYCVRGG
                     IIDLFAMGSVLPYRIDLFDDEIETIATFDVDTQRTLYPVPEVRLLPAREFPLDEAGQA
                     RFRQNFRDRFEGDPSKSRIYKDVSKGIAPSGIEYYLPLFFDKTATLFDYLPKNATLCL
                     HHDVDEAIATFGRDAASRYNLLRGDPQHPLLETKELFLDAEQFFTRLKEFARIDIVAT
                     QEGANLTPCATAAIPPIAVNRRAEIPTQAFQDFLKEYAGRVLLLADSLGRREIMSGYL
                     QEYGLAPAVCEDYAAFNAGKEKFMLGVGPLQNGFLLSDDDLALVTETELYAAQPRSRA
                     ARTAKKSNVEGMLRDLSELKVGDPVVHEQHGIARYQGLVNLDLGEGENEFLLLVYAGE
                     DKLYVPVAQLHVISRYSGGAADTAPLHKLGSGAWDKAKRRAMQQVRDTAAELLNLYAQ
                     RATRKGHQFKLTPHDYEAFAAGFGFEETPDQAAAIEAVINDLQSGKPMDRLVCGDVGF
                     GKTEVALRAAFVAAMDGKQVAILVPTTLLVEQHFQTFSNRFADLPVRIAELSRFRSTK
                     ETTQALKELAEGKVDIIIGTHKLIQKDVKFSNLGLVIIDEEHRFGVHQKERLKALRAE
                     VDVLTLTATPIPRTLAMSLEGLRDFSVIATAPQRRLSIKTFVAQAGNGVIREAVLREL
                     KRGGQVYFLHNEVETINNMAEKLTALLPEASIRIAHGQMNERELESVMRDFHHQRFNV
                     LLCTTIIETGIDVPTANTIIMNRADRFGLAQLHQLRGRVGRSHHQAYAYLLVETEALT
                     AQAKKRLEAIQAMEQLGSGFYLAMHDLEIRGAGEVLGESQSGEMQEIGFSLYTDMLNS
                     AISSLRQGHEPDMAHPLGVTTEINLHTPALLPNDYCGDIHQRLVIYKRLANCATQDDL
                     DDMHQELIDRFGLLPEPAQTLLDSHRLRIAAKPLGISKVDASSEAIQIQFVPNPPIDP
                     MKIITLIQSRRYIKMNGPDKLRIELKHEDLHQRVLAIMQFFKELI"
     gene            5967..6662
                     /gene="ispD"
                     /locus_tag="VFW59_08390"
     CDS             5967..6662
                     /gene="ispD"
                     /locus_tag="VFW59_08390"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5364276.1"
                     /translation="MSEFHALVPAAGFGARMGNDLPKQYLPLAGQPMIYHALTTLCAC
                     PDIATVFVVLAPDDTLFHGYDWSHCGEKLQPLYCGGATRAESVANGLLASELEPDDWV
                     LVHDAARPCVTQALLARLIAELRDDPVGGILAVPVADTLKRADAQQRIACTEPRAGLW
                     QAQTPQMFRAGLLAQALQQCKSVTDEASAIEALGLNPKLVASESTNFKVTYPQDLLLA
                     ELLLQHGKMPLAR"
     gene            6688..7161
                     /gene="ispF"
                     /locus_tag="VFW59_08395"
     CDS             6688..7161
                     /gene="ispF"
                     /locus_tag="VFW59_08395"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5364277.1"
                     /translation="MRIGQGFDVHQLVEGRRLIIGGVDIPYEKGLLGHSDADVLLHAV
                     CDALLGAAALGDIGKHFSDTDAKFKNIDSRILLRETMHLVREQGWRVANVDATIIAQA
                     PKMAPHIAQMVAHIAADLRVEKSAVNVKATTTEKLGYTGRGEGIAAQAVVLLLAN"
     gene            7161..7850
                     /gene="tsaB"
                     /locus_tag="VFW59_08400"
     CDS             7161..7850
                     /gene="tsaB"
                     /locus_tag="VFW59_08400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX5364278.1"
                     /translation="MRVLALETSTEYCSVALWQDGDVVSRCELVGQKHSELLMPMLDG
                     LLKEAGFRLQDVDGIAFGMGPGSFTGVRIACGVTQGLALGANLPVIGVCTLEALAEAS
                     AHDRVIAVLDARMSELYHAAYERQGDDWIAVSEPRLCKPECAPAVTGDGWFIAGSGLA
                     AHSAALQARYAGHLRGEDGAAVPQAAAIAALGARRFARGDSVDAAQALPLYLRDKVAL
                     KTHEREAARKS"
     gene            7847..8302
                     /gene="rimI"
                     /locus_tag="VFW59_08405"
     CDS             7847..8302
                     /gene="rimI"
                     /locus_tag="VFW59_08405"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5364279.1"
                     /translation="MTLRAMTEGDLDAVLAIEQSVQAYPWTRGNFSDSLNHGHLCRVD
                     EAEGELRGYVVLMPTVDEAELLTIGVAGTQQRKGLGRSMLLEMLGVVRGKNMQRVFLE
                     VRSSNAPAIALYRSAGFNEVGVRRGYYRNAEGSEDAIVMACELTGETNG"
     gene            8295..9248
                     /locus_tag="VFW59_08410"
     CDS             8295..9248
                     /locus_tag="VFW59_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5364280.1"
                     /translation="MDRQEAVLRELNLYPQWVLRNPPVVAEETLADAASSGTVRRAGS
                     EISPASMLAEPAVLEAAPLKIEQAMEPVAAAVAQVGEIAPVAVQAVAMMEVPLSEEYA
                     PDLPMPDDGHWGESMPDAVEAPSLLSTLDWTGLKQKVRDCTICKLRAGCSQTVFGVGD
                     EQADWLFVGEAPGEEEDARGEPFVGQSGMLLDNMLLALKLKRTHNVYIANIVKCHPPE
                     NRVPDAEEIAACLPYLHRQIELIQPRVIVALGKTAATALLGHRAALGPLRGTVHDFNG
                     IPLIATYHPAYLLRSPLDKARAWQDLCLAQKTILTQSGRQR"
     gene            9368..13075
                     /locus_tag="VFW59_08415"
     CDS             9368..13075
                     /locus_tag="VFW59_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5364281.1"
                     /translation="MLNGSRLYTRIAIAVSVALVAVGIALVSAEQARLRDAYLSERST
                     HLEKSLQAEELRLSQSIDTLRRDVVFLSNTPPIFGIMRATLNRGIDPRDGNTRAKWEA
                     RLQEIFSAFSKAHPVYYRIRYIGVADGGREIVSFDSRDARVANRNMTYGSREYFKRTL
                     GLRKDQVYLSEFEFQQEPDGSDNGRVQTLRAATPVYTSGGQIFGMVVLSLDVRPLLES
                     ARQGLSADVQTYIANSAGQYLLHPEAGRSFVFGTPDRDKITADFPLLDRMPQFSGDDA
                     SKHAMLNAANGQLLSVARLHFDPDSPGRFLWLLYRIPDVMGAQQRLMLPVAQLATGLG
                     LMLLLGIVALMLLRRAFAPLEQLKLAADQIAAGNHDIKLPENASGEIGALTRALNVML
                     RELSQREADILSINAELEQRVEQRTSDLALSNELLQSAVNESKALLQSAQLQSRRNQV
                     LMATSMDGIHVMDMQGNVVEANEAFCRMLGYSREEVAGLNVADWDVQWSTEELHERFE
                     FLVGKSELFETVHKRKDGSLIDVEITTSGVELDGASFLFASSRDITARKQAEIVMKQH
                     DMVISAAIDGYWMTDLQGYLLEVNQAYADLSGYAIDELVGMHISQLEAIEQSPEEVQA
                     HIAKVLAQGYDRFETQHRRKDGRSIDIEVSTTYLAEAERLFVFCRDISQRKRDEQTLR
                     IAAVAFDAQEAIVITDSHANIVRVNRAFEEITGYCAADVIGKNPRIMSSGRHDWRFYR
                     DMWQCLTENGSWAGEVWDRRKNGQIYPRWMTITAVRNESQIVTHYVGVFTDITERKRA
                     EEEIRNLAFYDALTGLPNRRLLLERLRAALFVSARHRNYGALLFLDMDRFKILNDTLG
                     HDFGDMMLIEVANRIKACVREMDTVVRLGGDEFVVLIEDVSTDAVDAAIKVGTVAEKI
                     REVLARPYLLGEHEHHSSPSIGVSLYHGNAESVNDLLQRADMAMYQVKKGGRNGVCIF
                     DAAMQVNAETNDALAKDLSRAIEQGELQLYFQIQVDEAQRPIGAEALLRWNQPQRGLM
                     LPDQFLPIAEKSLLILDIDRWVLNEACAQLARWGADERMRTLTLGVNISARLFAHPDF
                     VDYLAVMLGTYRIRPELLILESTECMLLSDFQEAAQKMRTLKALGVSLSMDDFGSGYS
                     SLTYLKQLPLDQVKVCKSFVQGVTLNDNDPLLVQAIIELGSKFGLSVLAEGVETEAQF
                     AFLRQHDCRLYQGFLFGKAVPVNEFEALFNP"
     gene            13261..14514
                     /locus_tag="VFW59_08420"
     CDS             13261..14514
                     /locus_tag="VFW59_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEX5364282.1"
                     /translation="MQKLSRPGKMVVFAALAIVLLLAWGYSRQQRAAPPIKIGVLHSL
                     TGTMAVSEAPLVDAVRLAVEEVNQSGGVNGRQIEIVAADCRSDAQYCAGEAERLIAGE
                     GVQALFGCWTSACRKAVKLVVEKHHHLLFYPLQYEGLEQSPNIVYTGAVPNQQVIPMV
                     TWALQQRGKRAYLIGSDYVYPRTANQIVKKLLQAQDGVLVAERYVPLGEKNLDEVARD
                     IAAQRPDFVLNTLNGGSNGHFFRALREAGVLASDIPVFSTSIAEAELAGIGPELVAGH
                     YVAWNYFQSLDTPKNKAFVARFKRRFGGDRVVDDPMEASYIGVKLWANALNAAETFDL
                     STIMNVMSMQTLAAPEGIVAVDYDTQHLWKPVLIGRARADGQFDIVWQSLANIAPAPF
                     PFFIPHAELQSMVRGDSQAGNGGAR"
     gene            14511..>15725
                     /locus_tag="VFW59_08425"
     CDS             14511..>15725
                     /locus_tag="VFW59_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX5364283.1"
                     /translation="MKISVRLLLFFLAVAALPLMLFSYLNLRQDEETLRTEAFGRMSD
                     LADKKAVQVRSYLAERVQDVRLLARGPRVMTAMAVLPGEYLPARRAGAAYAAEDANTH
                     QYFERYVEETGLFYDVFFISVQGEIVYTQKHETDFGSNLLTGSLRDSKFAQAFRAVRM
                     TMEPVISGYEIYEPSHAPALFIAAPVIVGGKLKGVFAVQLGNELLYRVANDATGLGIT
                     GEVTFAQHDGDGVLYTTPMKYRADAAMKYRVQGAELKSLPTSSATSGNSGAGSGPDYR
                     GIPVIAAWRYLPELEWGMVVKVDVDEVLEPIARQRMLLIEALAVCLLLAALTAYYLGR
                     RISRPIRALAQVADQVSAGRLDSRADENAPGELGVFAAAFNRMTVNLQELYRTLEDRI
                     DERTRELNATNEQ"
BASE COUNT         2909 a         4471 c         5178 g         3167 t
ORIGIN      
        1 cgaagccgct ctcctgtgcc tggaagaagg cctcttccca gtgtgcgcgc tgttcgggtt
       61 tctcggcctg agccagtccg cgcgcgacgc ggcggcgcac atcctcgacc gaggtctcgc
      121 ccggctcggc atatttttcc aatagcactt cggtgctgat ttcctgtgtc atcgttattc
      181 ctttttagtt gcggtctttg catccgcttc ttgagcgagt tcggcgcgga attcctccag
      241 cttgccgtgg tagtcgtccg cctcgccgta atcgaggaag tttttgtagc gtgcatgcgc
      301 ggcgcgcacc cggcgtgcgt gcttgatcgg gcagaagtac tgctcggtgc gcgcgatgat
      361 ctcggtcgca taggccagca gcccgaccgc gtacgagcaa tacaggcaat gcgccttctc
      421 gatgatgttc agataggcca gatgctggtg gtcgaacacg atgtagtcgc cgcgcttcac
      481 gcgggcgatg cggtaaaccg ggaagcagat cagctggtac aggctgaggc acagatcgag
      541 cagcgtcatc ggaatgatcg cgccgtagat gatgggcatg gtcaggtaat tctgcgggcg
      601 cacggtcagg aaccagcgga acacccccag cttgacgcgc cggtgcgcat cccgcacggc
      661 ttgttcgaac accacgcgtc ggccttcgag ctggtagagc aggcgctgct catgctcttc
      721 gatggccgca tccagttcgg tctcaagtgc ggtgatctgc tcggcgatct ggcgtatgcg
      781 gttattcatg gctgtttttt catggggagg ggcggcctaa cgatagccca atccagtcag
      841 gcatgggcga ttttatcggg atgtcaattt gcggacaaat tgtgtttgtt tatttatgcg
      901 accaggcgta cattttaccc agaaggcata gagttaatag tcagggagtg ataaatgaac
      961 gaagccattc cgatgcgccg tttcttcata ctgctcgggt tgatctacct gatcgaaatg
     1021 ctgctgatgc tcaccgtcga gcctgtgctg atttcggcca attatccgtt ctggataaga
     1081 gccatcgtcg attcggcact gcttgccatc atctgcgttc ccttcatgct gaactatcag
     1141 ctgcgcccga tgcagatggc ttaccacgac agcctgaccg gcttgcctaa ccgtctgctg
     1201 ttccacgaca ggctggagca tgcgctgctc gccgccaagc gcaacaagag cagttgcgcg
     1261 gtggtctttc tggatctaga tcgtttcaag ccggtcaacg atatctgcgg tcatcgtgtc
     1321 ggcgatgtgg tgctcaaaca ggcggcgtcc cgcatcgagg gctgcatgcg cgagagcgac
     1381 accgtggcgc gcatcggcgg tgacgaattc gccatcctgt tgtccggtcc ggttcaaaag
     1441 gcggatgccg atcaggtggt gcaaaagata tccgaagccc tgtccctgcc gttcgaggtg
     1501 cgcggcaagc ggctggatct cggagtcagt gcggggatcg ctttctatcc gcagaacgga
     1561 accgacggga tgacgctgct cgatgctgcc gacgatgcga tgtaccgcgc caaagaaatc
     1621 aagaaatcgt ctatctgcga cgcgatgcag ggcagcagaa tctagttgtc cagcaggttc
     1681 accagcccat ccagcccgac gaaattcagc gcatagctcg cctgttccct gaccaccggc
     1741 ttggcgtgat aggcgacact tagccccgcc tgagccatca tcttcagatc gtttgcgccg
     1801 tcgcccatcg cgatcacttg ctcgggctta agccccagct cgtcgcgcgt cttcaccagc
     1861 cagtcggcct tgctctgcgc atcgacgatc gctcccagca cccggccggt gagcttgccg
     1921 tcgacgatct ccagcgtgtt ggcgtgggca tggtcgaggc cgaggcgcgc cttcagccgc
     1981 tcggtgaaga atacgaagcc gccggatacc agcagcgtct tgatgccgtg ctgcttgagc
     2041 gccgccagca tgatcggcgc gcccggattc agttgcaggc gctcgtcgta gacgcgtagc
     2101 aaggcgcctt cgtccagccc ttgcagcaac gccacccggc ggcgcaggct ctcggcgaag
     2161 tcgatctcgc cgcgcatcgc ctcttcggtg atcgccgaga cctgcggttt cagtccctgc
     2221 atgtcggcga tctcgtcgat gcactcgatc gagatcagcg tcgagtccat gtccatcacc
     2281 accagtccga agtcggacag atgcttgccc tgcgccacga agccgcagtc gatctgttgt
     2341 tcaaggcaat agctagcgat gccgtcgtgc ggctttgctt cgcgcaggcg gtaggcgtgc
     2401 gggccgatct gctcgatgcg cgccgcattg gataatttgg caaggtgctc gacctgtgcg
     2461 gaagggatgt cgtgtagtgc ctggaggatg aggttcatta aatcagctct ttgaaaaatt
     2521 gcatgatcgc cagcacccgt tggtgcaggt cttcgtgttt taactcgatg cgcagcttgt
     2581 ccggcccgtt catcttgatg taacgccggc tctggatcag cgtgatgatc ttcatcgggt
     2641 cgatgggcgg gttcgggacg aactggatct ggatcgcttc cgaggaggcg tctaccttgc
     2701 tgatgcccag cggtttggcg gcgatgcgca ggcggtggct gtcgagcagg gtctgggcgg
     2761 gctcgggaag caggccgaaa cggtcgatca gttcctgatg catgtcgtcc agatcatcct
     2821 gcgtggcgca gtttgccaga cgcttgtaga tcaccagccg ctggtggatg tcgccgcagt
     2881 agtcgttggg cagcagcgcc ggggtatgca ggttgatctc ggtggtcacg cccaagggat
     2941 gggccatgtc cggctcgtgg ccctggcgca gcgagctgat cgccgagttg agcatgtcgg
     3001 tgtacagcga aaaaccgatc tcctgcatct cgccgctctg cgattcgccg agcacctcgc
     3061 ccgcgccgcg aatctcgaga tcgtgcatcg cgagatagaa gccgctgcct aactgttcca
     3121 tcgcctgaat cgcttccaga cgcttcttcg cctgcgcggt cagcgcttcg gtctccacca
     3181 gcagataggc gtaggcctga tggtgcgagc gtccgacgcg gcctctgagc tggtgcagtt
     3241 gcgccagacc gaagcggtcg gcgcggttca tgatgatggt gttggcggtc ggcacgtcga
     3301 tgccggtctc gatgatggtg gtgcacagca gcacgttgaa gcgctggtgg tggaagtcgc
     3361 gcatcaccga ttccagttcg cgctcgttca tctggccgtg cgcgatgcgg atgctggcct
     3421 cgggcagcag cgcggtcagc ttctcggcca tgttgttgat ggtctcgacc tcgttgtgca
     3481 ggaagtacac ctggccgccg cgcttgagtt cgcgcagcac ggcctcgcgg atcacgccgt
     3541 tgccggcctg tgcgacgaag gtcttgatcg acagtcggcg ctgcggcgcg gtggcgatca
     3601 ccgagaagtc gcgcaggcct tccagcgaca tcgccagcgt gcgcgggatc ggcgtggcgg
     3661 tcagcgtcag cacgtccacc tcggcgcgca gcgccttcag gcgttccttc tggtgcacgc
     3721 cgaaacggtg ctcctcgtcg atgatgacca gcccgaggtt ggaaaacttc acatccttct
     3781 ggatcagctt gtgcgtgccg atgatgatgt cgaccttgcc ttcggccaat tcttttaaag
     3841 cttgcgtggt ttccttggtg gaacggaagc gcgacagctc ggcgatgcgt accggcagat
     3901 cggcgaagcg gttggagaag gtctggaaat gctgctcgac caagagtgtc gttggcacca
     3961 gtatcgcgac ctgcttgccg tccatcgctg cgacgaaggc cgcgcgcagc gcaacctcgg
     4021 tcttgccgaa gccgacgtcg ccgcacacca gccggtccat cggtttgccg gattgcaggt
     4081 cgttgatcac cgcctcgatg gcggcggcct gatccggtgt ctcctcgaag ccgaagccgg
     4141 cggcgaaggc ctcgtaatcg tgcggggtca gcttgaactg gtggcccttg cgggtggcgc
     4201 gctgcgcata caggttaagc agctcggcgg cggtgtcgcg cacctgctgc atcgcgcggc
     4261 gcttggcctt gtcccacgcg ccgctgccca gcttgtgcaa cggtgcggtg tccgccgcgc
     4321 cgccgctgta gcggctgatc acatgcagtt gcgccaccgg cacgtagagc ttgtcctcgc
     4381 cggcatagac cagcagcagg aactcgttct cgccctcgcc cagatcgaga ttgaccaggc
     4441 cctgatagcg cgcgatgccg tgctgttcgt gcaccaccgg atcgccgacc ttgagttccg
     4501 acaggtcgcg cagcatgccc tcgacgttgc tcttcttcgc ggtgcgtgcg gcgcggctgc
     4561 ggggctgggc ggcatacagt tcggtctcgg tcaccagcgc gagatcgtcg tcggagagca
     4621 ggaagccgtt ttgcagcggc ccgacgccga gcatgaactt ctctttgcct gcattgaacg
     4681 cggcgtagtc ttcgcatacc gccggggcga ggccgtattc ctgcagatag cccgacatga
     4741 tctcgcgccg ccccaggctg tccgccagca gcagcacgcg ccctgcgtat tctttcagga
     4801 agtcctggaa cgcctgcgtg ggaatctcgg cgcggcggtt caccgcgatg ggcgggatcg
     4861 cggcggtggc gcagggcgtg aggttcgcgc cttcctgcgt ggcgacgatg tcaatccgcg
     4921 caaactcttt cagccgggtg aagaactgct ccgcatccag aaacagttcc ttcgtttcca
     4981 gcagcggatg ttgcgggtcg cctctgagca ggttgtagcg cgaggctgca tcgcgtccga
     5041 aagtggcgat ggcctcgtcc acgtcgtgat gcaggcacag cgtggcgttt tttggcaggt
     5101 agtcgaacag cgtcgcggtc ttgtcgaaga acagcggcag gtaatactcg ataccggagg
     5161 gggcgatgcc cttgctgaca tccttgtaga tgcgcgactt ggacgggtcg ccctcgaagc
     5221 ggtcgcggaa attctggcgg aaacgcgcct gtccggcctc gtccagcggg aactcgcgcg
     5281 cgggcagcag gcgaacctcc ggcaccggat acagcgtgcg ctgggtatcc acgtcgaagg
     5341 tggcgatggt ctcgatctcg tcgtcgaaca ggtcgatgcg gtagggcagc acgctgccca
     5401 tcgcgaacag gtcgatgatg ccgccgcgca cgcagtattc gccgggggtc agcacctgct
     5461 gcacatggtt gtaaccggcg aaggtcatct gttcgcgcag ttgcgcgaca tccagcttct
     5521 cgccgcgctt gaggaagaag gtgtaggcgg ccagatgctc gaccggcggc agcggataca
     5581 aggcggtcgt gatcggcacg atgaccacat cgcaggcgtt gctgcggata tggtgcaagg
     5641 tcgccaggcg ctccgagatc agatcctgat gcggcgagaa gtgatcgtaa ggcagcgtct
     5701 cccagtccgg cagcagatgc acgcgcagct tcgtgtcgaa gaacgggatc tcttccacca
     5761 gccgctgcgc ttccagcgcg ttggcggcga tcaccactaa tggcgagtgg gtcccggcat
     5821 attgcgccag cgccagcgca tcggacgaac cgtgcaaccg ggtgtaacgg ggacgggaat
     5881 gtttggaggt gaacagcatg taaacggcct gcaacaaagg cgcgcattat aagccaagga
     5941 aagcactttc atttacaatc gcggccatgt ctgaattcca cgccttggtt cccgccgccg
     6001 gtttcggcgc acgtatgggg aacgacttgc ccaaacaata tctgccgctg gccgggcagc
     6061 cgatgatcta tcacgcgctg acgacgctgt gtgcctgtcc cgatatcgcc acggtattcg
     6121 tggtgctggc acccgatgac acgctgttcc acggttacga ctggtcgcat tgcggcgaga
     6181 agctgcagcc gctgtattgc ggcggtgcga cgcgcgccga gagtgtggcg aacggcctgc
     6241 tggcctccga actggagccg gacgactggg tgctggtgca cgatgcggcg cgcccctgcg
     6301 tgacgcaggc gctgctggcc aggctgatcg ccgagctgcg cgacgacccg gtgggcggca
     6361 ttctggcggt gccggtcgcg gacacgctca agcgcgccga cgcacagcag cgcatcgctt
     6421 gcaccgagcc gcgcgcaggc ctgtggcagg cgcagacgcc gcagatgttc cgcgccggct
     6481 tgctggcgca ggcgttgcag caatgcaaaa gtgtcaccga cgaagcgtct gcgatcgagg
     6541 cgctgggcct gaacccgaag ctggtggcga gcgaatcgac gaatttcaag gtgacctacc
     6601 cgcaggatct gctgctggct gagttgctgc ttcaacacgg gaaaatgcct ctcgcccgct
     6661 agcgggcgag gggcggggga gagggtaatg cgtatcggac agggattcga cgtccatcaa
     6721 ctggtggagg ggcggcggtt gattatcggt ggggtggaca tcccctatga aaaagggttg
     6781 ctcggccatt ctgatgcgga tgtgctgctg catgcggtct gcgatgcgct gctgggagcg
     6841 gcggcgctgg gcgacatagg caaacatttt tccgacaccg acgcgaagtt caagaatatc
     6901 gacagccgca tcctgctgcg cgagacgatg catctggtgc gcgaacaggg ctggcgtgtc
     6961 gccaatgtgg acgccaccat catcgctcag gcgccgaaga tggcgcctca tatcgcgcag
     7021 atggtcgcgc atatcgccgc cgatctgcgc gtcgagaaga gcgcggtcaa tgtgaaggcg
     7081 accaccaccg agaagctggg ctacaccggt cgcggcgagg gcatcgcggc tcaggccgtc
     7141 gtattgctgc tggcaaactg atgcgcgttc tcgcactcga aacttccacc gaatactgtt
     7201 ccgtcgcgct gtggcaggac ggcgatgtcg tctcgcgctg cgagctggtc gggcagaagc
     7261 attccgaact gttgatgccg atgctggacg ggctgctgaa ggaggcggga ttcaggcttc
     7321 aggatgtcga cggcatcgcc ttcggcatgg ggccgggttc gttcaccggc gtgcgtatcg
     7381 cctgcggcgt gacgcaggga ctggcgctgg gcgcgaacct gccggtgatc ggggtgtgca
     7441 cgctggaggc cttggccgaa gcttcggctc acgacagggt gatcgcggtg ctggatgcgc
     7501 gcatgagcga gctgtaccac gcggcctatg aaagacaggg cgatgactgg atcgcggtga
     7561 gcgagccccg tctgtgcaag cctgagtgcg ctcctgcggt gacgggggat ggctggttca
     7621 tcgcaggcag cggtttggcg gcgcacagtg cggcattaca agcgcgctat gccggtcatt
     7681 tgcggggcga ggatggcgcg gcggtgccgc aggccgccgc gatcgccgcg ctgggcgcgc
     7741 gtcgctttgc ccggggcgat agcgtggatg ccgcgcaggc cttgcctctg tatctgcgcg
     7801 acaaggtggc gctgaagacc catgagcggg aagccgcccg aaagtcatga cgctgcgtgc
     7861 aatgaccgaa ggggatctcg atgcggtgct ggcgatcgag caatcggtgc aggcctatcc
     7921 ctggacgcgc ggcaatttca gcgactcgct gaaccatgga catctgtgtc gcgtggacga
     7981 ggccgaaggc gaattgcgcg gttatgtcgt gctgatgccg acggtcgacg aggccgagct
     8041 gctgaccata ggcgtggccg gcacgcagca gcgcaagggg ttggggcggt cgatgctgct
     8101 ggagatgctg ggcgtcgtgc gcggcaaaaa catgcagcgc gtgttcctgg aggtgcgcag
     8161 cagcaatgct cccgcgatcg ccttgtatcg cagcgcgggt ttcaatgagg tcggtgtgcg
     8221 gcgaggctat taccggaatg cggaaggcag cgaagatgcc atcgtgatgg cgtgcgaatt
     8281 gacgggtgag acgaatggat aggcaggaag cggtattgcg cgagttgaac ctgtacccgc
     8341 agtgggtact gaggaatccg cctgtggtgg cggaagaaac cttggctgat gccgcgagta
     8401 gcggcacggt gcgccgtgcc ggttctgaaa tatcacctgc ctccatgctg gcggagcctg
     8461 cggtgctgga agcggctccg ctcaagatcg agcaggctat ggagcctgtc gccgcagcgg
     8521 tggcacaggt tggcgagatt gcgccggttg cagtgcaagc ggtcgcgatg atggaagtgc
     8581 cgctgtccga ggaatatgcc cccgacctcc ccatgcccga tgacgggcac tggggggagt
     8641 cgatgcctga cgcagtcgag gcgccatcct tgctgagcac gctggactgg acaggactca
     8701 aacagaaagt ccgcgactgc accatctgca agctgcgcgc cggatgctcg cagacggtgt
     8761 tcggtgtggg cgacgagcag gccgattggc tgttcgtcgg cgaagcgccg ggcgaggagg
     8821 aagatgcgcg cggtgagccg ttcgtggggc agtcagggat gctgctcgac aacatgctgc
     8881 tggcgctcaa attgaagcgc actcataacg tgtatatcgc caacatcgtc aaatgccatc
     8941 cgccggaaaa tcgtgtgccg gatgcggagg agatcgccgc ctgcctgccc tatctgcatc
     9001 gtcagattga actgatccag cccagagtga tcgtggcgct gggcaagact gccgcaaccg
     9061 cgctgctggg tcaccgtgcc gcgctgggcc cgttgcgcgg cacggtgcat gacttcaacg
     9121 gcatcccgct gatcgccacc taccatccgg cctatctgtt gcgcagtccg ttggataagg
     9181 cacgtgcctg gcaggacttg tgtctggcac agaaaaccat cctgacgcaa tcgggccgcc
     9241 agcgttagta tcgaattcct gtttttctaa tcaaaggctt actctgggct aggaatggcc
     9301 tgagcgatgg ttttatgggg ttggaactgt agtagtatcg tcggacactg gttctggggt
     9361 gatccagatg ctgaatggtt cacgtttgta tacgcgcatt gcgattgctg tctccgttgc
     9421 tctggtagcg gtcggcatcg cgctggtatc ggccgagcag gcaaggctgc gcgacgccta
     9481 tctgagcgaa cgcagcactc atctggaaaa atccctgcag gcggaagagt tgcggctcag
     9541 tcagtcgatt gacacactgc gccgcgacgt ggttttcctg tccaacacgc cgcccatttt
     9601 cggcatcatg cgcgccaccc tgaaccgggg gatcgatccg cgcgacggca atacccgtgc
     9661 caagtgggag gcgcgtctac aggagatatt ctccgcattt tccaaggctc atcccgtcta
     9721 ttaccggata cgctacatcg gggtggccga tggcggcagg gagatcgtca gtttcgacag
     9781 ccgcgatgcc agagtcgcca acaggaatat gacttacggt tcgcgcgagt atttcaagcg
     9841 tacgctgggc ttgcgcaaag accaggttta tctatcggaa ttcgaattcc agcaggaacc
     9901 ggatggcagt gataacgggc gcgtccagac gttgcgtgca gccacgcctg tctacacctc
     9961 cggcgggcag atatttggca tggtggtact cagtctggac gtgcgccccc tgctggaatc
    10021 agccaggcag ggactgtcgg cggatgtgca aacctatatc gccaacagcg cgggacaata
    10081 tctgttgcat ccggaggcgg ggcgcagctt tgttttcggt acgccggatc gcgacaagat
    10141 caccgccgat tttccgcttc tggatcggat gccccagttc agcggcgacg atgcatcgaa
    10201 gcatgccatg ctgaatgcgg caaatggcca gttgctgtcc gtcgcacgcc tgcacttcga
    10261 tccggacagt ccggggcgct tcctgtggct gctgtaccgt atacccgatg tcatgggggc
    10321 gcagcagcgg ctcatgctgc ccgtggcaca acttgcaaca gggctgggat tgatgttgtt
    10381 gctcgggatc gtcgcattga tgttgctgcg cagagcgttt gcgccactcg aacaattgaa
    10441 gctggccgcc gatcaaatcg ccgcaggcaa tcacgatatc aaactgccgg aaaatgccag
    10501 cggcgagatc ggcgcgctga cgcgggcgtt gaatgtcatg ttgcgtgagc tgtcgcagcg
    10561 cgaggcggac atcctgagca tcaatgcgga actcgaacag cgggtggaac agcgcaccag
    10621 cgatctggca ctcagcaacg agttgttgca gtcggcggtc aacgagagca aggcgctcct
    10681 gcagagcgcg cagctgcagt cgcgtcgcaa tcaggtgttg atggcgacct cgatggatgg
    10741 catccatgtg atggatatgc agggcaatgt cgtcgaggcc aacgaggcat tctgccgcat
    10801 gctgggttac agccgggaag aagtcgccgg actgaatgtg gccgattggg atgtgcaatg
    10861 gtcgacggaa gagttgcatg aacgcttcga gttcctggtc ggcaagagcg agttgttcga
    10921 gaccgtgcat aagcgcaaag atggctcgct gatcgatgtc gagatcacca cctccggcgt
    10981 cgaactggac ggagcgtctt ttttgtttgc ctccagccgc gatatcaccg cgcgcaagca
    11041 ggcggaaatc gtgatgaaac agcatgacat ggtgatctcg gccgcgatag acggctactg
    11101 gatgaccgat ttgcagggct atctgctgga agtcaatcag gcctatgcgg atttgtcggg
    11161 ctatgcgatc gatgaactgg tcggcatgca tatcagccag ctggaggcga tcgaacaatc
    11221 gcctgaggag gtgcaggcgc atatcgccaa ggtgctggcg cagggctacg accgcttcga
    11281 gacgcagcac cggcgcaagg acgggcggag catcgatatc gaggtgtcga ccacctatct
    11341 ggccgaagcg gagcggctat tcgtgttctg ccgcgacatc agccagcgca agcgcgacga
    11401 gcagacattg cgtatcgccg cagtggcatt cgatgcccag gaggccatcg tgatcaccga
    11461 ttcccatgcc aacattgtcc gggtcaatcg ggcctttgag gagatcaccg gatactgtgc
    11521 cgccgatgtg atcgggaaaa atccccgcat catgagttcc ggacggcatg attggcgatt
    11581 ttatcgcgat atgtggcaat gcctgaccga gaacggttca tgggcgggcg aggtatggga
    11641 ccggcgcaag aacggccaga tctatccacg ctggatgacg atcaccgcag tcaggaacga
    11701 aagccagata gtgacccatt acgtcggcgt gttcaccgac atcaccgagc gcaaacgggc
    11761 ggaggaggag atccgcaatc tggcgttcta cgatgccctg accggtctgc ccaaccggcg
    11821 tctgctgctc gaacggttgc gtgccgcctt gttcgtttcg gcgcgccatc gcaactatgg
    11881 cgccttgctg ttcctcgata tggaccggtt caagatactc aatgacacgc tgggacatga
    11941 tttcggcgac atgatgctga tcgaggtggc caatcgcatc aaggcatgtg tgcgcgagat
    12001 ggataccgtg gtgagactgg gcggggacga attcgtggtg ctgatcgagg atgtcagcac
    12061 ggacgcggtg gatgccgcga tcaaggtcgg aacggtggcg gaaaaaatcc gcgaggtgct
    12121 ggcccggccc tatctgctgg gcgagcatga acatcatagc tcgcccagca tcggcgtctc
    12181 gctctatcac ggcaacgcgg agtccgtgaa cgacctgttg cagcgcgcgg atatggcgat
    12241 gtatcaggtg aagaaaggcg ggcgcaacgg ggtatgcatc ttcgatgccg cgatgcaggt
    12301 caacgccgag accaatgatg cgctggcgaa ggatctgagc cgcgcgatcg aacagggcga
    12361 gttgcagctg tacttccaga tacaggtgga tgaggcgcaa cgaccgatcg gtgccgaagc
    12421 tttgctgcgc tggaaccagc cgcaacgcgg actgatgctg ccggatcagt tcctgcccat
    12481 cgcggaaaaa agcctgttga tactggatat cgaccgctgg gtgctgaatg aggcttgcgc
    12541 acagcttgcc cgctgggggg ccgatgagcg gatgcggacg ctcacactgg gggtgaacat
    12601 cagtgcccgt ctgtttgccc atcccgattt tgtcgattat ttggccgtca tgctgggaac
    12661 ctatcgcatc aggcccgaac ttctgattct ggaatcgacg gaatgcatgt tactgagcga
    12721 ctttcaggag gccgcccaga agatgcgtac actgaaggcg ctcggcgtca gcctgtccat
    12781 ggatgatttt ggcagcggat attcgtcgtt gacctacctc aagcagttgc cgctggatca
    12841 ggtcaaggta tgcaagtcgt tcgtgcaagg cgtgacgctc aatgacaacg atcccttgct
    12901 ggtgcaggcg atcattgagc tgggtagcaa attcggtttg agcgttttgg ccgaaggggt
    12961 agagaccgag gctcagttcg cctttttgcg acaacacgat tgcaggctgt atcagggctt
    13021 cctgttcggc aaggcggtac cggtcaacga attcgaagcg ctgttcaatc cataggggca
    13081 ggccggatga gccgacacca tgatcgtgct catcggttac tcccgctggg gttgtttttc
    13141 tgcccgtagc ggcgaatcga gaagccttgc gcgggggcat ccagaattga tgaaacttcg
    13201 ctgcgagttt ggtagactga aattccgata gcctgcgttt gtatcctctt gaacaacatc
    13261 atgcagaaat tgtcccggcc cggaaaaatg gtggtgttcg ccgcgctggc gatagtgctc
    13321 ctgctggcgt ggggctattc cagacagcag cgtgccgcgc cacccatcaa gattggtgtg
    13381 ctgcattcgc tcaccggcac catggcggtg agcgaggcgc cgctggtcga tgcggtgcgg
    13441 ctggcggtgg aagaggtgaa ccagtcgggc ggggtgaatg gccggcagat cgagattgtc
    13501 gcggccgatt gccgttcgga tgcccaatac tgtgctggcg aggcggaacg gctcattgct
    13561 ggcgaaggcg tgcaggcact gttcggttgc tggacttcgg cctgccgcaa ggcggtcaaa
    13621 ctggtggtcg agaagcatca ccatctgttg ttctatccgc tgcaatacga ggggctggag
    13681 cagtctccca atatcgtcta taccggcgcg gtgcctaacc agcaggtgat tccgatggtg
    13741 acctgggcgc tacagcagcg tggaaaacgc gcctacctga tcggttcaga ttacgtctat
    13801 ccgcgcaccg caaaccagat cgtcaagaag ctgctgcagg cacaggacgg ggtattggtg
    13861 gccgagcgtt acgttccgct gggcgagaaa aatctggatg aggtcgcacg cgacatcgcg
    13921 gcacaacgac cggatttcgt gctgaacacg ctcaacggtg gcagcaatgg ccatttcttc
    13981 cgtgcgctgc gtgaggccgg ggtcctggcc tccgacattc cggtgttttc gaccagcatc
    14041 gccgaggccg aactggccgg aatcgggccg gagctggtgg ccggacatta tgtcgcgtgg
    14101 aattattttc agagcctcga caccccgaaa aacaaagcct ttgtggcaag gttcaaacgg
    14161 cgctttgggg gcgatcgcgt ggtggacgat ccgatggagg cttcctatat cggcgtgaaa
    14221 ctgtgggcga atgcgctcaa cgctgcggag acattcgacc tgtccaccat catgaatgtg
    14281 atgtcgatgc agacgctggc cgcaccggag ggcatcgtcg cggtcgatta cgatacccag
    14341 catctgtgga aaccggtgct catcggccgg gcgcgtgccg atggacaatt cgacatcgtc
    14401 tggcaatccc tggcgaacat tgcgcccgcg ccgttcccct tctttattcc gcatgccgag
    14461 ttgcaatcga tggtgcgcgg cgattctcag gctggcaacg ggggcgcacg atgaaaatat
    14521 ccgtgcgcct gctgttgttc ttccttgcgg tggccgcatt gccgctgatg ctgttcagct
    14581 acctcaacct gcgtcaggat gaagagacgc tgcgcaccga ggcatttggc cggatgtccg
    14641 atctggcgga caagaaggcc gttcaggtca ggagttatct ggcagagcgg gtgcaggatg
    14701 tgcgcctgct ggcgcgcggc ccgcgcgtga tgaccgcaat ggcggttctg cccggagaat
    14761 atctgcccgc gcggcgtgcg ggagcggcct atgccgccga agatgccaat acgcaccagt
    14821 atttcgaacg ctatgtcgag gagacggggc tgttctacga cgtgtttttt atttcggtgc
    14881 agggagagat cgtctatacc cagaaacatg agaccgattt cggcagcaat ctgctcaccg
    14941 gctccttgcg cgattcgaaa tttgcacagg ccttccgtgc cgtgcggatg acgatggagc
    15001 cggtgatctc cggctacgaa atctatgagc cttcgcatgc tcccgcattg tttatcgcag
    15061 cgccggtgat cgttggcggc aagttaaaag gcgtgtttgc ggtgcagctc ggcaacgagt
    15121 tgctgtaccg cgtggcgaac gatgccaccg ggctgggcat caccggcgag gtgaccttcg
    15181 cccagcacga cggcgacggg gtgctgtata caacgcccat gaagtatcgc gccgatgccg
    15241 cgatgaaata tcgcgtacag ggagcggaac tcaagagcct gccgacgagc agcgcgacca
    15301 gtggcaattc cggcgccgga agcgggccgg attaccgtgg tattccggtt attgccgcct
    15361 ggcgctatct gccggaactg gagtggggga tggtcgtcaa ggtggatgtg gacgaggtgc
    15421 tcgagccgat cgcccggcag cggatgctgt tgatcgaggc gctggcggtc tgcctgttgc
    15481 ttgccgcact gaccgcctat taccttggtc gccgcatcag caggccgatc agggccttgg
    15541 cgcaggtggc ggatcaggtg tctgcgggca ggctggacag tcgcgcggac gagaatgcgc
    15601 ccggcgagct gggcgtgttt gctgcggcat tcaaccggat gacggtcaac cttcaggagc
    15661 tgtaccggac gctggaggac cgtatcgacg aacgcacccg cgaactgaac gcgaccaacg
    15721 agcag
//
LOCUS       DASUIV010000230         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1174258, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000230 DASUIV010000000
VERSION     DASUIV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174258"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..1766)
                     /locus_tag="VFW59_08430"
     CDS             complement(336..1766)
                     /locus_tag="VFW59_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023098450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX5364284.1"
                     /translation="MKRFIEGTDRGQSTLFPECLQDWVDENNPVQAIDAFVDELDLAE
                     LGFDGVAPEVTGRPSYHPSILLKLYIYGYLNRVQSSRRLEREAGRNVEVMWLTGRLVP
                     DHKTIADFRKDNGGAIRQVCARFIVLCRAMGLFGEVSVAIDGSKFKAVNNRDKNFTRA
                     KMERRMAQIEESVARYLRQLDSADRQEPSGALTTKTTRLKEKIAKLKEEMRRLHRLKA
                     RMLATPDQQVSLVDPDSRSMATSGRGSGVVGYNVQVAVDIEHHLIIAHEVTNVGSDRA
                     QLARMAKETKATLERANLDVIADRGYFSSEEILECDRAGITVTLPKPMTSNPERFGKQ
                     DFRYVAGEDVYICPAGERLARSFTTQEHGLVLHRYLTNVCQSCAIKHRCTTAKERRIT
                     RWEHEHILEAVQRRLDEHPEKMRQRRETVEHPFGTIKARMGATHFLMKTLPRVASEMA
                     LHVLAYNFTRVMNIIGIQPLMAAIRA"
BASE COUNT          416 a          638 c          611 g          458 t
ORIGIN      
        1 cagttcggac tccagagaag gttccctgaa gtgcgcactc gcattgcgct gatagccgat
       61 caccggcata gctgtgtgct gcgcgcccgc cgaaagcggc cacgccgccg ccgcgccgcc
      121 gagcagggag atgaactcgc gccgctccat ggactgcccg cctcaagccg ggagccgcac
      181 agtaccatac cgttgtgcga gaatgctgcc gtgcagtgcg gcaaatttga tcgtcgaatg
      241 gcagagatgg gtcttggtcg tgtcaaaacg ccttgggata aggggcagtg cggattttgg
      301 tggctttcgc gaatgggagc ctgcttttgg gcggactatg ccctgatcgc tgccatgagc
      361 ggctgaatgc cgatgatgtt catgacgcgg gtgaagttgt aggccagcac gtgaagtgcc
      421 atctctgagg cgacgcgcgg cagcgtcttc atcaggaagt gtgttgctcc catccgggct
      481 ttgattgtgc cgaatggatg ctcgaccgtt tcgcgccgct ggcgcatctt ctctggatgt
      541 tcgtcaaggc ggcgctgaac ggcttcgaga atgtgttcat gctcccatcg cgtgatccgt
      601 cgctctttgg cggtggtgca gcgatgctta atggcgcaac tctgacagac gttggtcaga
      661 tagcggtgca acaccagtcc atgttcttgg gtcgtgaatg agcgcgcgag ccgctcccca
      721 gcaggacaga tgtaaacgtc ctcccccgcc acgtagcgga agtcctgctt gccgaagcgc
      781 tctgggtttg aggtcatcgg cttcggcaag gtgactgtga tgcctgctct gtcgcattcc
      841 aggatttctt cgctgctgaa gtagccgcgg tcagcgatga catcgagatt ggctctttcc
      901 agcgtcgctt tcgtctcctt ggccatgcgc gcaagctgcg cccggtcgct cccgacgttg
      961 gtcacctcat gtgcgatgat cagatgatgt tcgatgtcga cggcgacctg gacattgtag
     1021 ccgacaacac ccgatcctcg cccgctggtt gccatcgagc gagaatcagg atcaaccagc
     1081 gatacctgct ggtcgggcgt cgccagcatc cgcgccttaa gtctatgcag gcgccgcatc
     1141 tcctccttca gcttcgcgat cttctccttc aagcgtgtcg tcttggtcgt gagcgccccc
     1201 gacggctcct gccgatcggc gctgtcgagc tggcgcagat agcgggcaac gctctcctca
     1261 atctgcgcca tccgccgctc cattttcgcc cgagtaaagt tcttgtcccg gttgttcact
     1321 gccttgaact tgctgccatc aatcgcgacg ctgacctcac cgaataggcc cattgctcga
     1381 caaagcacaa tgaaccgggc gcacacctgg cgaatcgccc cgccattgtc cttgcggaag
     1441 tcagcaatcg tcttgtgatc aggaacaagg cggcccgtca gccacatcac ctcgacgttg
     1501 cgaccagcct cacgttcaag ccggcggctc gactgaaccc gattgagata tccgtagatg
     1561 tagagcttca gcaggatcga tgggtgatac gagggacgtc cggttacctc aggagcgacc
     1621 ccgtcaaagc ccaattccgc cagatcaagt tcatcaacaa aggcatcgat cgcctgaacg
     1681 ggattgttct cgtcgaccca gtcttgcagg cactctggaa acagcgtgct ctgtccgcga
     1741 tccgtaccct caatgaagcg cttcatgcga tcctccgcag cagcagaatc acactacgga
     1801 gtgaatcaca gccggcacgt ttttacacag ccagggtcaa ccacgaccaa gtcagttaag
     1861 gccgaatctc cgacaatgtc cgcttatgct ccgatagtta ccggaccagc gcgcatcagc
     1921 aaaacgacga gaaaggccag attcagacgt gagcagatgc agcaatatat gtgcagacgc
     1981 caagcttatt cgatcacctc gtcggcgcgg ccgagcagcg ccgggggcag cgtgagggcg
     2041 aatatctttg ccgtcttctg gttgatcagg agctcgaatt tggtggcctg ataaatcgga
     2101 atgtcacccg gcttggcacc ttt
//
LOCUS       DASUIV010000231         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1175181, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000231 DASUIV010000000
VERSION     DASUIV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175181"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="nagZ"
                     /locus_tag="VFW59_08435"
     CDS             complement(<1..659)
                     /gene="nagZ"
                     /locus_tag="VFW59_08435"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX5364285.1"
                     /translation="MGLGPVMLDVVGKTLTAEDEQRLRHPLVGGVILFARNYDSPGQL
                     AELTAAIRAVRSPPLLIAVDHEGGRVQRFREGFTKIPAMRELGKIWDAHPQRARHLAQ
                     QAGYVLAAELRACGVDFSFTPVLDVDYGQSSVIGDRAFHSDPEAIAELAHSLLLGLKQ
                     AGMHTVGKHFPGHGYVKADSHLDIPVDEREFVDIEMCDLVPFQRMIDYGLSAVMPAHV
                     I"
     gene            complement(817..2163)
                     /gene="xseA"
                     /locus_tag="VFW59_08440"
     CDS             complement(817..2163)
                     /gene="xseA"
                     /locus_tag="VFW59_08440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX5364286.1"
                     /translation="MSEGLFLEEKNRVLRVSELNLAIKRLLENEVPLLWVRGEISNLV
                     KASSGHFYFSLKDDQAQVRCVMFRHKSQILGEPLSNGQQVEVRAVATLYEQRGDFQLT
                     VEQLRPAGLGVLYERFAQLKQRLEREGLFDAERKRELPRFPRRIGVVTSPQAAALRDV
                     LTTLRSRLPGVPVVLYPTPVQGAGSADKIALAIATANQRAECDVLIVCRGGGSIEDLW
                     SFNEQVVAYAIADSTIPVVSGVGHETDFTIADFVADERAPTPTAAAQLVVPDRRELLR
                     GLGDLAQHLQRAQRNRLQNAMQTVDFLQRRLVHPAQQLQRQALQLAQLHQRLQRAYAY
                     RHQHQHWQWLSLARRMQAASGDYGRMQERQSNLAERMTRAMRAAQQRDASRLDNVAQH
                     LIYLDPKQVLARGYSMVQGADGGVVSDASALTVGEALRVTFAKGWVKAEVKETGEK"
BASE COUNT          546 a          785 c          667 g          470 t
ORIGIN      
        1 tagatcacat gcgcgggcat caccgccgag aggccgtagt cgatcatgcg ctggaacgga
       61 accaggtcgc acatctcgat atcgacgaac tcgcgctcgt ccaccggtat gtccagatgc
      121 gaatcggcct tcacataacc atggccggga aaatgcttgc cgaccgtatg catgccggcc
      181 tgcttcaacc ccagcagcag gctgtgcgcc agctcggcaa tcgcctccgg atcgctgtga
      241 aaagcgcggt cgccgatcac gctgctctgc ccgtagtcca catccagcac gggcgtaaag
      301 ctgaagtcca cgccgcaggc gcgcaactcc gctgccagca cataacccgc ctgctgcgcc
      361 agatggcgcg cacgttgcgg atgagcatcc catatcttgc cgagctcgcg catcgccgga
      421 attttggtga aaccttcgcg aaaacgctgc acccgcccgc cctcgtgatc taccgcaata
      481 agcagcggcg gggaacgcac cgcgcgtatc gccgccgtca gctcggccaa ttgccccggc
      541 gagtcgtagt tgcgtgcaaa cagaatgacg ccgccaacca gcggatggcg taaacgttgt
      601 tcatcctctg cggtgagcgt cttgcccacc acatccagca tcaccggccc caaacccata
      661 gcgactcctg atcgatttaa cagctgcgga ttataagggt tttagcggag gaaccgaggt
      721 ggacgcacaa ggaatcggcg tgtggccacc gccgttttga atttggtttg atgcgggtca
      781 tgcatgtttc gatacggcct actgaacaag aacggcttac ttttcccccg tctccttgac
      841 ctctgccttc acccatccct tggcaaacgt aaccctcaac gcctcgccga ctgtcagagc
      901 cgaggcatcc gacaccacgc ccccatccgc cccctgcacc atgctatagc cccgtgccag
      961 cacctgcttc ggatcgagat agatcaggtg ctgcgcgaca ttgtccagcc gcgaggcatc
     1021 gcgctgctgc gcggcacgca tcgcgcgtgt catgcgttcg gccagattgg actgacgttc
     1081 ttgcatgcgt ccataatcgc cgctggcggc ctgcatgcgg cgcgccagcg atagccattg
     1141 ccaatgctgg tgttgatgac ggtaagcgta ggcacgttgc aggcgctggt gcaattgcgc
     1201 cagctgcagc gcttggcgtt gcaattgttg ggcgggatgc accagccggc gttgcagaaa
     1261 gtccaccgtc tgcatcgcat tctgcaggcg gttgcgttgg gcgcgttgca ggtgttgtgc
     1321 cagatcgccc agcccccgca acaattcgcg ccgatccggc accacaagct gggcggcggc
     1381 ggtgggggtt ggcgcgcgtt cgtcggcgac gaaatcggca atggtgaaat cggtctcgtg
     1441 cccgactccg ctgacgacag gaatggtgct gtcggcgatg gcataggcca caacctgttc
     1501 gttgaacgac cacagatctt cgatgctgcc gccgcctcga cagacgatca gcacgtcgca
     1561 ttccgctcgc tgattcgcgg tggcaatcgc cagcgcgatc ttgtccgcgc tgcccgcgcc
     1621 ctgcaccggg gtcggataaa gcaccaccgg cacgcccggc aggcgtgatc gcaaggtggt
     1681 gagcacatcg cgcagcgcgg cggcctgcgg tgaagtgacg acaccgatgc gccgggggaa
     1741 tctcggcaac tcgcgcttgc gctcggcatc gaacaggcct tcgcgttcca gacgctgctt
     1801 gagttgcgcg aaacgctcat acagcacacc caaccccgcc ggacgcagct gctcgacggt
     1861 cagctgaaaa tcgccgcgct gttcatataa ggtagcaacc gcccgcacct cgacctgctg
     1921 accattgctc aagggctcgc ccaatatctg gcttttgtga cggaacatca cgcaacgcac
     1981 ctgcgcctga tcgtccttca gcgaaaaata gaaatggccg gaagatgcct ttaccagatt
     2041 ggatatttcg ccgcgcaccc atagcagcgg cacctcgttc tcaagcaaac gcttgattgc
     2101 aaggttaagt tccgagacgc gcaagacgcg atttttttct tccaaaaata gaccttcaga
     2161 catattgaca atccagaaac aatccacatc ccgcgaaaat acggcgcttc acgccgccca
     2221 ccccgatgca caaacaaata cttagcgtaa ccaattgttt taaataagaa ttaaagcaac
     2281 tcaaaaaaca ggcaggacaa gaaaatccct tatacgacgg caccttaaac aggcatccag
     2341 ccagttaatc cacaaagtta tccacagctt ttgtgggtaa acgacaaaaa tccttatcag
     2401 atacagactt agtactaatc tgcctaagtt aaccctgaga aacgacgatc cagcaatagc
     2461 cggaagcg
//
LOCUS       DASUIV010000232         5224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1268380, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000232 DASUIV010000000
VERSION     DASUIV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268380"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..469)
                     /locus_tag="VFW59_08445"
     CDS             complement(56..469)
                     /locus_tag="VFW59_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364287.1"
                     /translation="MSVTSTSGTQPVTQAAFQQLQLAQARQFAERAAQNARSLQASAN
                     SAQAAADQAQENAKSLSVDASQAHDVALQAARNVQSTESANQIGPQVLGTITQAAQTP
                     AVAVPAPTPSSTPAPAQPTVNTQGQTIGTVINVTA"
     gene            752..1096
                     /locus_tag="VFW59_08450"
     CDS             752..1096
                     /locus_tag="VFW59_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEX5364288.1"
                     /translation="MKKLIAVVCFGFFFIVSSSVFAGGQQEKMKGCNKEAKGAALKGD
                     ERKAFMKKCLSKDYVLKANGAAAAATPAVAEPAAPASTQQDKMKSCNADAKTKGLKGE
                     ERKVFMSSCLKG"
     gene            1219..2562
                     /gene="pcnB"
                     /locus_tag="VFW59_08455"
     CDS             1219..2562
                     /gene="pcnB"
                     /locus_tag="VFW59_08455"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX5364289.1"
                     /translation="MIKKILKRVFRKSPKVKSVGNAQVIPFELHGVPRDQISFGAKRV
                     TDGLQAAGYQAYVVGGAVRDLLLERTPKDFDVATDATPEEIKRVFRRSRIIGRRFRLV
                     HVMFGEEVVEVSTFRSMIEAEDAETDAHGRLLRDNQFGDQEQDAARRDFTANALFYDP
                     STQEIYDYHRGYADTRDHLLRVIGDPATRYREDPVRMLRAVRLSAKLGIKLDAATAAP
                     IAKMKDLLDNVPQARLLDEVLKMLLSGHSVECIQQLRKMNLHQGLLPLLDVILEQPVG
                     EKFVMLALRNTDERLAQEKSVSPSFLFAALLWHEVLATWKKYQQQGERPIPAMHAAMD
                     EVLARQRAQLAIPHRHDAVMKEVWLMQLRLEQRTGQRPFRLLEQPRFRAAFDFLLLRC
                     ASGEVDDELGLWWDEFQDASNERRAEMLQPEGAGESKRRRRKPRKKTVAGAEPKA"
     gene            2562..3056
                     /gene="folK"
                     /locus_tag="VFW59_08460"
     CDS             2562..3056
                     /gene="folK"
                     /locus_tag="VFW59_08460"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5364290.1"
                     /translation="MLHTAFIGLGSNLERPSSQLQRAFADIDSLPDTRLVAHSGLYRS
                     APVGYLDQPDFVNAVAQVVTGLSPQSLLQSLLQIEHDHGRERSFRNAPRTLDLDVLLY
                     DDLQLHEHGLTIPHPQMHLRAFVLQPLLEISPDCIIPGVGKAEMVLSACQDQVLERLA
                     DVAG"
     gene            3043..3687
                     /locus_tag="VFW59_08465"
     CDS             3043..3687
                     /locus_tag="VFW59_08465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX5364291.1"
                     /translation="MSPDRYRYVVVEGPIGVGKTSLAHRLARHLGAATLLEKPDENPF
                     LAKFYQDPTRHALATQLFFLFQRGNEVRDLAQMNLFHSGTVADYLLDKDMLFARLNLN
                     EEEFALYQQIYHTLQLQAPVPDLVIYLQAEPATLIERVRRRAKYYEQTISDAYLSRLT
                     QSYSDFFYHYDEAPVLMVNSEHLNFVDGDEDFTLLLRRIEEMRGPREFFSRGQS"
     gene            3872..4666
                     /gene="panB"
                     /locus_tag="VFW59_08470"
     CDS             3872..4666
                     /gene="panB"
                     /locus_tag="VFW59_08470"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5364292.1"
                     /translation="MRTTLTALQALRKKGEKIAVLTCYDASFAALLEAQGVDVLLVGD
                     SLGMVLQGHETTLPVTLRDMIYHTECVARGAKQTFIVSDMPFGTSQVSPQETFAHAAQ
                     LMAAGAQMVKLEGGAAMVETVQFLTGRGIPVCAHIGLTPQSVHQLGGYRVQGKESAAA
                     QQLLQDAVALEQAGASMVLLEAVPALLAAEITAALAVPTIGIGAGADCSGQVLVLHDM
                     LDIFPGKKARFVKNYMQGAASIADAISRYVVEVKSGSFPAAEHSFA"
     gene            4715..>5224
                     /locus_tag="VFW59_08475"
     CDS             4715..>5224
                     /locus_tag="VFW59_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEX5364293.1"
                     /translation="MTIPSRHEAQQRADQIHVFRDELECLEQEGVVALEPEQRRRVDE
                     HHRNLLAQYAQHFDIDRDRQSSQLSRGMRIASLFGALALSASVFFLFYQFWGWFSMEA
                     QVVVLIAAPLCMLGLTVGIQCKDVSGYFAKLAALVTFACFVLDITMLGQIFNITPSDK
                     ALLPWAALAF"
BASE COUNT         1007 a         1339 c         1657 g         1221 t
ORIGIN      
        1 cggagctaac gggattgatg gccatgatcg atctcctgtc gttgaaatta aatcatcaag
       61 cggtcacgtt gatcactgtg cctatcgtct ggccttgagt gtttaccgtg ggttgcgcag
      121 gtgcgggtgt cgagcttggt gtcggagccg gcactgcaac ggctggtgtt tgagctgctt
      181 gagttatcgt tccgagaacc tgtggcccga tctggtttgc agactctgtg gattgcacgt
      241 tgcgagctgc ctgcaacgca acgtcatgcg cctgactggc atctacgctc agggatttgg
      301 cattttcctg cgcttgatct gcggctgcct gagcgctgtt ggcgctcgct tgtaaggagc
      361 gggcattttg cgcagcgcgc tctgcaaact gccgcgcttg cgccaactgc agttgctgaa
      421 aagcagcttg tgtaacgggt tgtgttcccg atgtcgaggt aacgctcatg atctgcgtac
      481 tcctgattac ttcctcatga ccttcggttg caaatatacg cctaaaactc ttctgaattc
      541 aagtggattt tgttgtttct gggggatgtg cggcgtggcg ccggttttct tgcgcgcttc
      601 gtcaggaaag gtttttccac tcaggacgga ttatctaggt aattcagtct attgacggga
      661 aggcgggagg ggattagggt gccaaacggg ggcgtgccga agcttgttgg cccccgtttt
      721 tgtcgccatc attaattctt tggaggtcgc tatgaaaaag ttgattgcag tggtttgttt
      781 cggattcttt tttattgttt catcttcagt atttgcaggc gggcagcagg agaagatgaa
      841 gggatgtaat aaggaagcaa agggagccgc ccttaagggg gacgaacgca aggctttcat
      901 gaagaagtgt ctgtcgaagg actatgtttt gaaggcaaac ggcgcggctg ctgcggcaac
      961 tccggcagtt gcagagcctg ctgcaccagc gtctacgcag caggacaaga tgaagtcctg
     1021 caatgccgat gccaagacca aggggctgaa aggggaagag cgcaaggtgt tcatgagttc
     1081 ttgtctgaag ggctgatttg tatccgcttg atcgagacgc gccgcaaggc gcgttttttc
     1141 attgcgggga gaaaaaacga tgcggtgtat aattgcaatt ctgtttgcgc cgagtcgcgc
     1201 catatcaatc aataacgcat gatcaaaaaa attcttaaac gtgtattccg caagtcgcca
     1261 aaggtcaaat cggttggtaa tgcgcaggtg attccattcg aactgcatgg cgtgccgcgt
     1321 gatcaaatca gttttggtgc caagagagtg actgatggct tgcaggccgc tggctatcaa
     1381 gcctatgtgg tgggcggtgc agtgcgtgat ctgttgctgg agcgcacgcc aaaagatttc
     1441 gacgtggcga ccgatgctac accggaggaa attaagcgtg tgtttcgccg ttcacgcatt
     1501 atcggacggc gtttccggct ggtgcatgtg atgttcggcg aggaagtagt cgaggtttcg
     1561 actttccgca gtatgatcga ggccgaagat gccgagactg acgcacatgg ccgtttgctg
     1621 cgtgacaatc agttcggtga tcaggagcag gacgcggcgc ggcgcgattt caccgcaaat
     1681 gcgctgttct atgatccttc cactcaggaa atctacgact atcaccgtgg ttatgccgat
     1741 actcgcgatc atttgctgcg tgtgatcgga gatcctgcga cccgttatcg cgaggatccg
     1801 gtacgcatgc tgcgggctgt gcgcctgtcc gccaagctgg gcatcaagct ggatgccgcg
     1861 acggctgcgc cgatcgccaa gatgaaggac ctgctcgaca atgtgcctca ggcgcgtttg
     1921 ctcgatgaag tgttgaagat gttgttgtca gggcattcgg ttgaatgcat ccagcagttg
     1981 cgcaaaatga atctgcatca gggtttgctg ccattgctgg atgtgattct ggagcagcct
     2041 gtgggcgaga agttcgtgat gctggcgttg cgcaataccg acgagcgcct cgctcaggaa
     2101 aaatcggtct caccttcgtt cctgtttgct gcattgctgt ggcatgaagt gctggcgacc
     2161 tggaaaaaat atcagcagca aggcgagcgc ccgattccgg cgatgcatgc ggcaatggac
     2221 gaggtgttgg ccagacagcg tgcgcagctc gcgattccgc atcgtcacga tgcggtgatg
     2281 aaggaggtct ggttgatgca gttgcgtctg gagcagcgca ccgggcagcg cccattccgt
     2341 ttgctggagc agcctcgttt ccgtgcggcc ttcgattttc tgttgttgcg ctgtgccagt
     2401 ggcgaggtgg acgacgagct gggcttatgg tgggacgagt ttcaggatgc cagcaatgaa
     2461 cggcgcgccg agatgttgca gccggaaggt gcgggcgaga gtaaacgtcg ccgccgtaaa
     2521 ccgcgcaaaa aaactgtggc gggcgcagag ccgaaagcgt aatgctgcac accgccttca
     2581 tcgggctggg tagcaatctg gagcgcccaa gcagccaatt acagcgcgcc tttgccgaca
     2641 tcgacagctt gccggacacg cggctggtcg cgcattccgg gctgtatcgc agtgcgccag
     2701 tgggttatct ggatcagcca gacttcgtca atgcggtggc acaagttgtc accggtctct
     2761 cgcctcagtc gctgttgcaa tcgttgctgc agatcgagca tgaccatggt cgcgagcgtt
     2821 cttttcgcaa tgcgccgcgt acgctggatc tggatgtgct gctgtacgac gatctgcaat
     2881 tgcatgaaca tggtctgact attccgcatc ctcagatgca tctgcgtgcc ttcgtgttgc
     2941 aaccgctgct ggagatttcc ccggattgca tcattccagg cgtgggtaag gcggagatgg
     3001 tgttgagtgc gtgccaggat caggtgttgg agcggcttgc cgatgtcgcc ggataggtat
     3061 cgttatgtgg ttgtagaagg gccgattggt gtgggcaaaa ccagcctcgc gcatcgcctc
     3121 gccaggcatc tcggtgcggc aactctgttg gagaagccgg atgagaatcc ctttcttgcc
     3181 aagttctatc aggacccgac gcgccatgcg ctggcgacac agctgttctt tctgttccag
     3241 cgcggtaacg aggtgcgcga tctggcacaa atgaacttgt tccatagcgg aaccgtggcg
     3301 gactacctgc ttgacaagga tatgctgttt gcgcgactga atctgaacga ggaggagttt
     3361 gcgctgtatc agcagattta tcacaccttg cagttgcaag ctcccgtgcc cgatctggtg
     3421 atctatttgc aggccgagcc ggcaacgctg atcgagcgag tgcggcgtcg cgccaagtac
     3481 tatgagcaga ccatctccga tgcttatctg tcgcggctga cgcaaagcta tagcgatttc
     3541 ttctatcatt acgacgaagc gccggtgttg atggtgaaca gcgagcacct taatttcgtc
     3601 gatggcgacg aggattttac cttgctgttg cggcgcatcg aggagatgcg cgggccgcgc
     3661 gaatttttca gcagggggca gagttgaaaa aggtatttca cgattgctct ttctcccgga
     3721 gattcaatat tgcctttctc gttagcttcc tcgcccgctt gcgggagagg attgaggtaa
     3781 gggttgaccg ggtaaccagc gacttgcacg gctctatcgt gcttggtcga atggctagtt
     3841 gtctcaccag aaagttggac agggggctat gatgcgaacc acattgactg cactgcaagc
     3901 cctgcgcaaa aagggcgaga agatcgccgt gctgacctgt tatgacgcga gcttcgccgc
     3961 gctgctcgaa gcgcaggggg tggacgtact gctggtgggc gattcgctcg gcatggtgtt
     4021 gcaggggcat gaaaccacgc tgccggtcac gctgcgcgac atgatctacc acaccgaatg
     4081 cgttgcgcgc ggtgccaagc agacgttcat cgtcagcgac atgccgttcg gtacttccca
     4141 ggtcagcccg caggagacct tcgctcatgc cgcgcaactg atggcggcgg gcgcgcagat
     4201 ggtcaagctg gaaggcggcg cggcgatggt cgagacggtg caattcctca ccgggcgcgg
     4261 catcccggta tgcgcccaca tcgggctgac cccgcaatcg gtgcaccagt tgggcggcta
     4321 tcgcgtgcag ggcaaggaat ccgccgccgc gcagcagttg ttgcaggacg cggtggcgct
     4381 cgaacaggcc ggggccagca tggtcttgct ggaggctgtg ccggccttgc tggcggcgga
     4441 aatcaccgct gcgcttgccg tccccaccat cggcatcggc gcgggcgcgg actgttccgg
     4501 gcaggtgctg gtgctgcacg acatgctgga tattttcccc ggcaaaaagg cgcgcttcgt
     4561 gaagaattac atgcaggggg ctgcctcgat cgcggatgcg atatcccgct atgtcgtcga
     4621 ggtgaagagc ggcagctttc cggctgccga acatagcttc gcctgatttt ccgcacggtt
     4681 gtggcacatc cctgaaaact ttcgggagtc atgaatgact attccttcgc ggcatgaggc
     4741 gcagcagcgc gctgatcaaa tccatgtctt tcgggacgag ctggagtgtc tggagcaaga
     4801 gggcgttgtc gcgctggagc cggagcagcg ccgccgcgtg gacgagcatc accgcaatct
     4861 gctcgcgcag tacgcgcagc atttcgatat agaccgggac aggcaatcca gccagttgtc
     4921 gcggggcatg cgcatcgcgt cgctgttcgg cgcgctggcg ttgtccgcaa gcgtgttctt
     4981 cctgttctac caattctggg gctggttttc gatggaggcg caagtggtcg tgctgatcgc
     5041 cgcgccgctg tgcatgctgg gcctcacggt cgggatacag tgcaaggatg tctcgggata
     5101 tttcgccaag ctggcggcgc tggtgacgtt cgcctgtttc gtgctcgaca tcacgatgct
     5161 ggggcagata ttcaatatca cgccgtccga caaggcgctg ctgccctggg ctgcgctggc
     5221 gttc
//
LOCUS       DASUIV010000233         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_430926, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000233 DASUIV010000000
VERSION     DASUIV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430926"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="VFW59_08480"
     CDS             complement(<1..2287)
                     /locus_tag="VFW59_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HEX5364294.1"
                     /translation="MQQIAKPGWLGLHPSFQNTLLNMVKILLVLMGAQLMAWLMPALP
                     EFKGIPNYLPLHVLLETISVVVSMMVFAVGWNSRSSNLSGNAVLLACVFFSVGMLDFL
                     HTVSYGGMPDFISPNDSQKHLNFWLSARFFASVILLVVAIRPWRPLGFRATRYVIFGS
                     LILVTALINWTVVYHQAWLPDTFVLGQGLTAFKKNSEYSIIALNIVTAVILWNKMREP
                     QTFNVVLLFGAACTLAMSEFFFTLYTTMTGSYNVLGHVYKVIAYLFIYRAIVVETIEE
                     PYNKLALAQQNLATSLKASNTGLWDWNLRTKEVFFSPEWKAQLGYLPDELPDRFSTWE
                     SLLHPDDVGPAVERVKNFLASSNRQYENEFRLRHRDGSYRWIMARGERQFDSNGTCVH
                     LIGSHIDISERKQAEQRIQQLANFDMLTGLPNRMLFEERVKQAIGIAKRERTHLSILF
                     FDLDHFKDINDTLGHHIGDEVLVEVGKRLKSTVRDLDTVSRMGGDEFMLLMPDSDKNG
                     VAQVAKALLVSIAKPYLIEQSEFVVTPSVGIAMYPEDGEDFDTLYRHADTAMYSAKRD
                     GRNNYRFFTQEMQSRTQRTLKIENALRYAIERNELHLYYQPQLTIADTRLIGVEALLR
                     WQHPELGAISPAEFIPIAEKSGQITRIGEWVLRTAVQQLKAWLDSGFPPMIMAVNLSV
                     VQFRHPDLPGLVTSILEEAQLPPECLELELTEGVAMDEPERAITVMDDLHARGVRMSL
                     DDFGTGYSSLNYLKKFSIYKIK"
     gene            complement(2401..>3181)
                     /gene="selD"
                     /locus_tag="VFW59_08485"
     CDS             complement(2401..>3181)
                     /gene="selD"
                     /locus_tag="VFW59_08485"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX5364295.1"
                     /translation="SDVYAMGGTPIMALAIVGMPINKLPVETIRAILQGGESVCKAAG
                     IPLAGGHSIDSVEPIYGLVALGIVHPDKLKKNSDAQAGDVLILGKGIGVGVLAAALKK
                     EALSPAGYAQMIAATTQLNAVGSKLSTLDGVHALTDVTGFGLLGHLLEVCRGSGLTAE
                     LKFDRVPVLSEALPLAQQGYGPGAIDRNLASYGQDIDFAPQLETWQRRLLADAQTSGG
                     LLVSVAAAEADEVLRCFREAGFGQAAVIGAMRQGAARVAVS"
BASE COUNT          684 a          969 c          904 g          624 t
ORIGIN      
        1 tcttgatctt gtatatgctg aacttcttca gataattgag cgacgagtag cccgtgccga
       61 agtcatcgag cgacatgcgc acgccgcgcg catgcaaatc gtccatcacc gtgatggcac
      121 gttccggctc atccatggca acgccttcgg tcagctccaa ttccagacac tcgggaggca
      181 attgcgcctc ttcgaggatg ctggtaacca accccggcag gtcgggatga cggaactgca
      241 ccacagacag gttgacggcc atgatcatcg gcggaaaacc gctgtccaac caggctttga
      301 gctgctgaac ggcagtacgc agcacccatt cgccgatccg ggtgatttgc ccgcttttct
      361 ccgcgatcgg aatgaattct gcgggcgata tcgcgcccaa ctcgggatgt tgccagcgca
      421 gcaaggcttc cacgccgatc aggcgggtat cggcgatggt cagttgcggt tgatagtaca
      481 gatgcaactc attccgttct atcgcgtaac gcagcgcgtt ttcaattttc agcgtgcgtt
      541 gagtgcgtga ctgcatctct tgagtgaaga aacggtaatt gtttcgtcca tcccgtttgg
      601 cactgtacat cgcggtatcg gcgtgccggt aaagcgtgtc gaaatcctcg ccgtcttcgg
      661 ggtacatcgc gatacccacc gaaggggtca ccacgaactc gctctgctcg atgaggtagg
      721 gtttggcgat cgacaccagc aaggccttcg cgacctgggc cacgccgttc ttgtcgctgt
      781 ccggcatcaa cagcatgaat tcgtccccgc ccatccggga aacggtatcc agatcgcgca
      841 ccgtggattt cagccgcttg cccacctcga ccaatacctc gtcgccgatg tggtgcccca
      901 aggtatcgtt gatatctttg aagtgatcga ggtcaaaaaa cagtatggac agatgcgtgc
      961 gctccctctt cgcgatgccg atcgcctgtt tgacacgctc ctcgaacagc atcctgttgg
     1021 gcaagccggt gagcatatcg aaattagcca gttgctggat acgctgctcg gcttgtttgc
     1081 gttcggaaat atcgatatgc gagccgatga gatggacgca ggtgccattg ctatcgaatt
     1141 gcctttcgcc gcgcgccatg atccagcgat aactgccgtc gcggtgacgc aggcggaact
     1201 cgttctcata ttgtcgattc gacgaggcca ggaaattctt caccctctcc acggctggcc
     1261 cgacgtcgtc gggatggagc aatgactccc aagtggaaaa ccggtcaggc aattcgtcgg
     1321 gcagataacc cagctgcgct ttccactccg gcgagaagaa cacctctttg gtacgcagat
     1381 tccaatccca cagcccggta ttggaggctt taagggaggt cgcaaggttc tgctgtgcca
     1441 gagcgagctt gttatagggc tcttcgatcg tctcgactac gatggcgcgg taaataaaca
     1501 gataggcgat caccttgtag acatgaccca gcacgttgta actgcccgtc atggtggtgt
     1561 agagggtaaa gaagaattcg ctcatcgcca aggtgcatgc ggccccgaac aacaggacca
     1621 cgttgaaggt ctgcggctcg cgcatcttgt tccacaggat gacggcggtg acgatattga
     1681 gcgcgatgat cgaatattca gagtttttct tgaatgccgt gagcccctgc cccagcacga
     1741 aggtatccgg caaccacgcc tgatggtaga cgacggtcca attgatcaag gcggtgacga
     1801 gaatcaatga accgaagatg acataacgcg tggcgcgaaa gccaagcggc cgccatggtc
     1861 tgatggccac gaccagcagg atcaccgatg caaaaaaacg cgccgacagc cagaaattga
     1921 ggtgcttttg cgagtcgttg ggcgaaatga aatccggcat gccgccgtag gacacggtgt
     1981 gcaaaaagtc gagcatgccc accgaaaaga acacgcaggc aagcagcacg gcattcccgg
     2041 aaagattgct gctgcgcgag ttccagccga ccgcgaacac catcatggaa acgacgaccg
     2101 agatggtctc cagcaagaca tgcagcggca ggtaattggg aatgcctttg aactccggca
     2161 acgcgggcat cagccaggcc atcagttgcg cgcccatcaa caccagcaga atctttacca
     2221 tgttgagcag ggtattttga aagctgggat gcagccccaa ccagcccggc ttagcgattt
     2281 gctgcatgta aaattcctga atttttatcg aattgaaagg ccgcgcattc tacccgagca
     2341 ctcaggcagc agccgtcgac aaggtcacga agccccgcag aaaaacgcat tgccctgcct
     2401 tcagctcacc gccacccgtg ccgcgccctg acgcatcgca ccgatcaccg ccgcctgccc
     2461 gaagcccgct tccctgaaac agcgcagcac ttcgtcggcc tccgctgccg cgacgctcac
     2521 cagcagaccg ccgctggtct gggcatcggc cagcaggcgg cgctgccagg tttccagttg
     2581 cggggcgaaa tcgatatcct gaccgtagct cgcgaggttg cggtcgatcg cgccggggcc
     2641 gtagccctgt tgcgccagcg gcaaggcttc ggacagcacc ggcacccgat cgaatttaag
     2701 ctcggcggtc aggcccgaac cgcgacagac ctccagcagg tggcccagca gaccgaagcc
     2761 ggtcacgtcg gtcagcgcat gcacgccgtc cagcgtggat aatttgctgc ccacggcatt
     2821 gagctgggtg gtcgcggcga tcatctgcgc gtaacccgca ggcgacaagg cctccttctt
     2881 cagcgccgca gccagcacgc ccacgcctat ccccttgccc aggatgagca catcgcccgc
     2941 ctgcgcgtcg ctgttcttct tcagcttgtc gggatgcacg atgcccagcg ccaccagacc
     3001 atagatcggc tcgaccgaat cgatcgagtg cccgcccgcc agcgggatgc cggcggcctt
     3061 gcacaccgac tcgccgccct gcaggatcgc gcgtatggtc tcgaccggca gcttgttgat
     3121 cggcatgccg acgatggcca gcgccatgat gggggtgccg cccatcgcgt acacatcgga
     3181 c
//
LOCUS       DASUIV010000234         7395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_987471, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000234 DASUIV010000000
VERSION     DASUIV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987471"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFW59_08490"
     CDS             complement(<1..192)
                     /locus_tag="VFW59_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5364296.1"
                     /translation="MSKIKIAIAGCGGRMGRALLEGVAQSDDLMLHAALEHAGSALLG
                     QDASVFGGTRGVAITADVEA"
     gene            complement(189..926)
                     /gene="bamE"
                     /locus_tag="VFW59_08495"
     CDS             complement(189..926)
                     /gene="bamE"
                     /locus_tag="VFW59_08495"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX5364297.1"
                     /translation="MRIKLILVSVLLASCSSLSTPLLSPYKMDIRQGNFISPEMRDKL
                     KLGMSRQQVRYVLGTPMVSDVFHENRWDYAYRLEQGGKLVEEQHLTAFFEGDNLVRVE
                     DNGHVLEGLAAMVPDPVVEPVKEVAKADPGVAVQASVQAWAAAWSAKNVPDYLAAYTS
                     DYAPKGMSRKKWEKLRLDRISRPKVIEVTLSDASVSLQDDDHATVSFIQNYRSDAYRD
                     VVQKTLSLVRQEGRWLIAAEEVTKDKK"
     gene            complement(972..2633)
                     /gene="recN"
                     /locus_tag="VFW59_08500"
     CDS             complement(972..2633)
                     /gene="recN"
                     /locus_tag="VFW59_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX5364298.1"
                     /translation="MLKFLGIRDFVIVSSLELDFSGGFTALTGETGAGKSILIDALSL
                     ALGGRGDASMVRNGCERAEIAAEFDISGLPLLSDWLREQELEGDAGVCLLRRVLDAGG
                     RSRGFINGCSATLQQMREAGEQLLDIHGQHAHQSLLRPDAQRALLDGYAGMATEAEQV
                     AAQYREWQALHRRRIALSENAEAMAAERELLQFQRDELKGLGFNAAAWQTLQEDHARL
                     SHAAGLLETAAFGVDTLSEADNACLAQLNALTSRLRDGMQHDAALGDTLRMLESAQAE
                     LQEAVYALRHYQQRLDADPQRLRELEQRIQEVMDAARKYRVAPEQLNETLQEVESRLA
                     ELGGDADLAALEQQENVARQAYMTAAQKLSAARGKAAAKLAQEITAAMQTLAMEGGSF
                     AVALLPLAEGNVCGLESIEFQVAANPGVPPRSLAKVASGGELSRISLAIQVATSQVAS
                     VPTLIFDEVDSGIGGRVAEIVGHLLKRLGKNYQVLCVTHLPQVAAAADQQWQVSKAVE
                     EGSTLSRITVLDDERRIEEIARMLGGVTITETTRKHAAEMLGAPA"
     gene            complement(2654..3526)
                     /locus_tag="VFW59_08505"
     CDS             complement(2654..3526)
                     /locus_tag="VFW59_08505"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX5364299.1"
                     /translation="MKFPFQNVALIGKYKAPEIAEPLLRLAAFLTSGGLQVVVDNLTA
                     DHLADNSYPVMTLEEMTGVVDLAIVIGGDGTMLNIARTLSPHHIPLIGVNQGRLGFLT
                     DLTLDNMQESIAAMLEGKFVTEQRLLLSAKVLRDGAEVFGGLAFNEIVVHRSQISSMV
                     EFEVRIDGEYLYNQRADGLIVSTPTGSTAYALSAGGPILHPSLDVLELVPVCPHSMSN
                     RPIVVKSSSLLEILMQRTGDVRVRFDSHTTYDLQLHDKIVVTRHPELACLLHPVGHSY
                     YHTLREKLLWNQTL"
     gene            3749..4075
                     /gene="trxA"
                     /locus_tag="VFW59_08510"
     CDS             3749..4075
                     /gene="trxA"
                     /locus_tag="VFW59_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX5364300.1"
                     /translation="MSENIHYVSDATFDAEVLQSPLPVLVDYWAEWCGPCRMIAPILD
                     EIAKEYAGRLTVAKLNVDENQQTPQKFGVRGIPTLMLFKNGNIEATKVGALSKSQLTA
                     FIDSHI"
     gene            4251..5507
                     /gene="rho"
                     /locus_tag="VFW59_08515"
     CDS             4251..5507
                     /gene="rho"
                     /locus_tag="VFW59_08515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5364301.1"
                     /translation="MHLSDIKLKHVSELIEMAAANQIENANRMRKQDLIFALLKSEAK
                     KGESIYGDGTLEILPDGFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDTIEGEIR
                     TPKDGERYVALVKVDKVNDEPPENTKNKILFENLTPLFPTVPLRLERDIRAEENVTGR
                     IIDIVAPIGKGQRGLLVASPKSGKTVMLQHIAHSISSNNPDAVLIVLLIDERPEEVTE
                     MSRTVRGEVVASTFDEPASRHVQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VIPSSGKVLTGGVDANALHRPKRFFGAARNIEEGGSLTIIATALVDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMAEKRIYPAINVNRSGTRKEELLIKADILQRIWLLRKLLYPMDE
                     LEAMEFLLDKIKATKNNAEFFDSMKR"
     gene            5670..5981
                     /gene="rplU"
                     /locus_tag="VFW59_08520"
     CDS             5670..5981
                     /gene="rplU"
                     /locus_tag="VFW59_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5364302.1"
                     /translation="MYAVIKTGGKQYRVIAGETLKIETIPGDVGAAIVLDKVLMVADG
                     DKLSMGKPLLTGASVTATIVSHGRGEKVHIFKMRRRKHYKKSQGHRQNYTEIRIDGIS
                     A"
     gene            6003..6260
                     /gene="rpmA"
                     /locus_tag="VFW59_08525"
     CDS             6003..6260
                     /gene="rpmA"
                     /locus_tag="VFW59_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5364303.1"
                     /translation="MASKKGGGSTSNGRDSHSKRLGVKSYGGELINAGSIIVRQRGTE
                     FHPGDNVGMGKDHTLFAKITGKVVFAIKGAQKRRTVSIVAA"
     gene            6375..>7395
                     /gene="obgE"
                     /locus_tag="VFW59_08530"
     CDS             6375..>7395
                     /gene="obgE"
                     /locus_tag="VFW59_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX5364304.1"
                     /translation="MKFIDESKIEVFAGSGGNGVASFRREKYIDKGGPDGGDGGRGGS
                     VFAQADRNINTLVDFRFTRTHRARNGEAGRGSDCYGKGADDVILRMPVGTVITDINSG
                     EVIADLARDGEIALLAQGGKGGLGNIHFKSSTNRAPRQCTPGEPGEERELQLELKVLA
                     DVGLLGMPNAGKSTFIRAVSAAKPKVADYPFTTLHPNLGVVRVSHEKSFVIADIPGLI
                     EGAAEGAGLGHQFLRHLARTRLLLHLVDIAPMYEGTDPVAEARAILNELKKYDQALYE
                     KPRWLVLNKLDLLENRDEKVAAFLKEFSDFDRHFAISAITGEGCKELTYAIMDHVQKT
                     TDQERE"
BASE COUNT         1584 a         2336 c         2077 g         1398 t
ORIGIN      
        1 cgcctcgaca tccgccgtga tcgcaacgcc gcgcgtgccg ccgaacacgc tggcatcctg
       61 ccccagcagc gcgctgccgg cgtgttccag tgctgcgtgc agcatcaggt catcggattg
      121 cgccacgcct tccagcaatg cgcgccccat gcggccaccg catccggcga tggcgatttt
      181 gatcttgctc atttcttgtc cttagtaact tcttcagcgg cgatcagcca acgcccctcc
      241 tgccgcacca gcgatagcgt cttctgcacc acatcgcgat aggcatcgga acggtaattc
      301 tggatgaacg acaccgtcgc atgatcgtcg tcctgcagac tgacgctggc gtcgctcaag
      361 gtaacctcga tgaccttggg acggctgatg cgatccagcc gcagcttctc ccatttcttc
      421 cggctcatgc ccttcggcgc atagtcggag gtgtaggccg cgagataatc gggcacattc
      481 ttcgccgacc atgctgccgc ccaggcctgc acgctggcct gcaccgccac gcccggatcg
      541 gctttggcga cttccttgac tggctcgacc accggatcag gcaccatcgc cgccaaacct
      601 tccagcacat ggccgttatc ctcgacgcgc accagattat cgccctcgaa gaaggcggtg
      661 agatgctgct cctctaccag cttgccgccc tgctccagcc gataggcata gtcccagcgg
      721 ttctcgtgga acacatcgct gaccatcggc gtgcccagca cgtaacgcac ctgctgtctg
      781 gacatgccca gtttgagctt gtcgcgcatc tccggcgaga tgaagttgcc ctgacggatg
      841 tccatcttgt agggcgacag cagcggggtg gacaggctgc tgcacgaagc gagcagcacg
      901 gagaccagaa tcagttttat acgcataggg ttctcacttc gtttcaatat ttttcgccgc
      961 agcatgatac ctcatgcggg cgctcccagc atttcagcgg catgcttacg cgtggtctcg
     1021 gtgatggtca caccgcccag catgcgggcg atctcctcga tgcgacgctc gtcgtccagc
     1081 accgtgatcc ggctcagcgt gctgccctct tccaccgcct tgctcacctg ccattgctga
     1141 tcggctgccg cagccacctg cggcagatgc gtgacgcaca acacctgata attcttgccc
     1201 agacgcttga gcagatgtcc gacgatctcg gccacgcgcc cgccgatgcc gctgtccacc
     1261 tcgtcgaata tcagcgtcgg cacgctggcg acctgactgg tggcaacctg gatcgccaga
     1321 ctgatgcgcg acaattcgcc gccggaggcc accttcgcca gactgcgcgg cggcacgccg
     1381 ggattggccg cgacctgaaa ctcgatgctc tccagtccgc agacattgcc ttccgccagc
     1441 ggcaacagcg ccacggcaaa gctgccaccc tccatcgcca gcgtctgcat cgcggcggtg
     1501 atctcctgcg ccagcttggc ggcggccttg ccgcgcgccg cactcaattt ctgcgccgcc
     1561 gtcatataag cctgacgggc aacattttcc tgctgctcca gcgccgccag atcggcgtcg
     1621 ccgccgagct ctgccaggcg tgactcgact tcctgcaagg tctcgttcaa ttgttccggt
     1681 gcgacgcggt atttgcgcgc ggcatccatc acctcctgaa tgcgctgctc cagttcgcgc
     1741 aggcgctgcg gatcggcatc caggcgctgc tgataatggc gcagggcgta caccgcctcc
     1801 tgcaattcgg cctgcgcgct ttccagcatg cgcagcgtat cgcccagcgc ggcgtcgtgc
     1861 tgcatgccgt cgcgcaggcg cgaagtgagc gcattgagct gtgccagaca ggcattgtcc
     1921 gcctcgctca gggtgtcgac gccgaaggcg gcggtctcca gcaggcccgc cgcatgagac
     1981 agacgcgcat gatcttcctg caaggtctgc cacgcagcgg cattgaagcc caggcctttc
     2041 agctcgtcgc gctggaactg caacaattcc cgctcggcgg ccatcgcctc ggcattctcg
     2101 ctgagcgcga tgcgccggcg atgcagagcc tgccattcac ggtactgcgc agcgacctgc
     2161 tccgcctctg tcgccatgcc cgcataaccg tcgagcagcg cacgctgggc atcggggcgc
     2221 agcaaagact ggtgcgcatg ctggccgtgg atatcgagca gctgttcgcc cgcttcgcgc
     2281 atctgctgca aggtggcgct gcaaccgttg atgaagccgc gcgaacgtcc gcccgcatcc
     2341 agcacccggc gcagcagaca gacacccgcg tcgccctcca gctcctgctc gcgcaaccag
     2401 tcgctcaata aaggcaggcc ggagatatcg aattcggcgg cgatctcggc gcgctcgcag
     2461 ccgttgcgca ccatggaggc atcgccccgt ccgcccagcg caagcgacaa ggcatcgatc
     2521 agaatggact tgcccgcacc ggtctcgccg gtgagcgcgg tgaagccgcc ggaaaaatcc
     2581 agttcgagcg aggagacgat cacgaaatcg cgtataccca gaaacttcag cattgaatag
     2641 gggagttcat tgattacagg gtctgattcc acagcagctt ttcacgcagc gtgtggtaat
     2701 agctgtggcc aaccggatgc agcaggcagg caagctcggg atggcgcgtg acgacgatct
     2761 tgtcgtgcag ctgcagatca taagtcgtat ggctgtcgaa tcgcacccgg acatcgccgg
     2821 tgcgctgcat cagaatctcc agcagcgacg agcttttcac cacgataggc cgattgctca
     2881 tcgaatgcgg gcacaccggc accagttcga gcacatcgag gctgggatgc aatatcggcc
     2941 cgccggcgga cagcgcatag gctgtcgagc cggtgggggt ggacacgatc aatccgtcgg
     3001 cgcgctggtt atacagatac tcgccgtcga tgcgcacctc gaactcgacc atgctgctga
     3061 tctggctgcg atgcaccacg atctcgttga atgccagccc gccgaacacc tcggcgccat
     3121 cgcgcaacac cttggcagag agcaacaggc gctgctcggt gacgaacttg ccttcgagca
     3181 tcgccgcgat gctctcctgc atgttgtcca gcgtcaggtc ggtcagaaaa cccaggcgcc
     3241 cctgattcac gccgatcagc gggatatggt gtggcgacag cgtgcgggcg atgttgagca
     3301 tggtgccatc gccgccgatc acgatcgcca gatcgaccac gccggtcatc tcctccaggg
     3361 tcatcaccgg ataagaattg tcggccagat gatcggcggt cagattgtcc accacgacct
     3421 gcaagccgcc ggatgtcaaa aaggcagcca gccgcaacaa cggctcggcg atctccggag
     3481 ccttgtattt gcctattaac gcgacattct ggaagggaaa tttcatggtg ggcgattaaa
     3541 ccacatcgcc tccataatga caaaataggc catttggtta aaacggccgc aatgccgata
     3601 aatcaagggg agattcccgg ccacttcaca gggggcgttt ttttggttag gatgccgcat
     3661 cgcatttgat gccaacctct tcaggcaaga ttatttccag gctgtacttt tgttttacct
     3721 tccctaattc ccagaacgag acacaaacat gagcgaaaac atccattacg tatctgacgc
     3781 aactttcgac gcagaagtat tgcagtcccc tctgcccgtg ctggtggact actgggccga
     3841 atggtgcggc ccatgccgca tgatcgcacc gatcctggat gagatcgcca aggagtacgc
     3901 tggccgcctg accgtcgcca agctgaacgt ggatgaaaac cagcagactc cgcaaaaatt
     3961 cggcgtccgc ggcatcccga cgctgatgct gttcaagaat ggcaacatcg aagccaccaa
     4021 ggtcggcgcc ctgtcgaagt cgcaattgac ggcatttatt gacagccaca tctaaagcgt
     4081 ttactgtcgc acccgcattg cccttccgat tcggaaatca gactcatcct ccgcagttcc
     4141 aaatccgaga cgaattcgtt ccaggccatg cgggtggttc accacccggc accaccccat
     4201 aaactcacac cattcctttc gtcacttcca gactccatct gcgcatcgct atgcatctat
     4261 ccgatataaa actaaaacac gtttccgaat tgatcgaaat ggctgcggcc aaccagatcg
     4321 aaaatgccaa ccgcatgcgc aagcaggatt tgatcttcgc gctgctgaag agcgaggcga
     4381 aaaagggcga aagcatctat ggcgacggca cgctggagat cctgccggac ggcttcggct
     4441 tcctgcgctc ccctgacacc tcctatctgg ctggcccgga cgacatctat gtcagcccca
     4501 gccagatccg ccgcttcaac ctgcacaccg gcgacacgat cgagggcgag attcgcaccc
     4561 cgaaagacgg cgaacgctat gtcgctctgg ttaaggtgga caaggtcaac gacgagccgc
     4621 ccgagaacac caagaacaag attctgttcg agaacctgac gccgctgttc ccgaccgtgc
     4681 cattgcgcct cgagcgcgac atccgcgccg aagagaacgt caccggccgc atcatcgaca
     4741 tcgtcgcgcc gatcggcaaa ggtcagcgcg gtctgctggt ggcctcgccc aaatccggca
     4801 agaccgtgat gttgcagcac atcgcccatt cgatctcgtc caacaacccg gatgccgtgc
     4861 tgatcgtgct gctgatcgat gaacgtcccg aggaagtgac cgagatgagc cgcaccgtgc
     4921 gcggtgaagt ggtcgcctcc accttcgacg aaccggccag ccgccatgta caggttgcag
     4981 aaatggtgct ggaaaaggcc aagcgcctgg tcgagcacaa gaaagacgtg gtcatcctgc
     5041 tcgactccat cacccgtctg gctcgcgcct acaacaccgt gatcccgtct tcgggcaagg
     5101 tattgaccgg cggcgtggat gccaacgcat tgcatcgccc caaacgcttc ttcggcgcgg
     5161 cacgcaatat cgaagaaggc ggctcgctga ccatcatcgc caccgcgctg gtagacaccg
     5221 gctcgcgcat ggatgacgtg atctacgagg aattcaaggg caccggcaac atggagatcc
     5281 atctggaccg ccgcatggcc gagaagcgca tctacccggc gatcaacgtc aaccgttccg
     5341 gcacccgcaa ggaagagctg ctgatcaagg ctgacatcct gcaacgcatc tggctgctgc
     5401 gcaaactgct ctacccgatg gacgaactgg aagcaatgga gtttctgctc gacaagatca
     5461 aggcaaccaa aaacaatgcc gagttctttg actccatgaa acgctaaccc gcgccgggaa
     5521 agcaattagg tctcgctatg cgagtttcac aataattttg acggggccgg atatttatgt
     5581 ataatccgcg ccttcgcaaa aacagagttt cagagaaggc taaccttcgg gttatgccgt
     5641 aactaaaacc gcactcaatc gaggttgtta tgtacgcagt tattaaaacc ggtggtaagc
     5701 agtatcgtgt tatcgccgga gaaaccctga aaattgaaac catccccggc gatgtgggcg
     5761 ctgctattgt cctggacaag gtgttgatgg ttgccgatgg cgacaagctg tccatgggca
     5821 agccgttgct gactggcgca tccgtgacag ccaccatcgt gtcgcatggc cgtggtgaga
     5881 aggtgcacat cttcaagatg cgtcgccgta agcactacaa gaaatcgcaa ggtcatcgtc
     5941 aaaactacac cgagatccgt atcgacggca tctccgcgta attctgttaa ggagcatata
     6001 acatggcatc gaaaaaaggc ggcggcagta ccagtaacgg ccgcgactca cactcgaaac
     6061 gactgggcgt taaaagctac ggcggcgaac tgatcaacgc gggcagcatc atcgtacgtc
     6121 aacgcggcac cgagttccat cctggcgaca acgtcggcat gggcaaggac cacactctct
     6181 ttgccaagat caccggcaag gtagtgttcg ccatcaaggg cgcgcaaaag cgtagaacag
     6241 tttccatcgt cgccgcgtaa gcagcgtcgt ttgaagcgaa tcagccctat cgcaaggtag
     6301 ggctttttta tttggttttt cgatgagatc ggcggcgccg agccattgaa ggacaaacag
     6361 ggcttgaaca aacaatgaag ttcatcgacg aatccaagat cgaagtgttc gcaggcagcg
     6421 gcggcaacgg cgtggccagc ttccggcgcg aaaaatacat cgacaagggc ggtccggacg
     6481 gcggtgacgg cggacgaggc ggtagcgtat ttgcgcaagc ggatcgcaac atcaatacgc
     6541 tggtggactt ccgtttcacc cgcacccatc gagcccgcaa cggcgaagcg ggacgcggtt
     6601 cggattgcta cggcaaaggg gcggatgacg tcattctgcg catgccggtg ggcaccgtga
     6661 tcaccgacat caacagcggc gaggtcatcg ccgatctcgc ccgcgatggc gaaatcgccc
     6721 ttctggcaca aggcggcaag ggcggactgg gcaatatcca cttcaaatcc agcaccaacc
     6781 gcgcaccgcg ccaatgcacg ccgggcgaac ccggcgaaga acgcgaattg caactcgaac
     6841 tcaaagtctt ggccgacgta ggtctgctgg gcatgccgaa cgcgggcaaa tccaccttta
     6901 tccgcgccgt ttccgccgca aagcccaagg tggccgacta cccgttcacc acgctgcacc
     6961 cgaatctggg cgtggtgcgc gtgtcgcatg aaaagagctt cgtcattgcc gatattcccg
     7021 gcctgatcga gggtgccgcc gagggcgccg gtctgggcca tcagttcctg cgccatctgg
     7081 cccgcacccg cttgctgttg catctggtcg atatcgcgcc gatgtatgaa ggaaccgatc
     7141 ccgtcgccga ggcgcgtgcc atcctgaacg agctgaagaa atacgatcag gcgctgtatg
     7201 aaaaaccgcg ctggctggtg ctgaacaaac tggatctgct ggagaatcgc gacgagaagg
     7261 tcgccgcctt cctcaaggaa ttttccgatt tcgaccgtca cttcgcgatc tctgcgatca
     7321 ccggcgaagg ctgcaaggaa ttgacctacg cgatcatgga tcacgtacaa aagacaaccg
     7381 atcaggaacg ggaag
//
LOCUS       DASUIV010000235         3488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1175480, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000235 DASUIV010000000
VERSION     DASUIV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175480"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1764)
                     /gene="secA"
                     /locus_tag="VFW59_08535"
     CDS             complement(<1..1764)
                     /gene="secA"
                     /locus_tag="VFW59_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX5364305.1"
                     /translation="MISRALKSIFGSRNDRLLKQYRATVQSINKLEAGVAKLSDDELR
                     QKTDSFRQRFASGESLDALLPEAFAVVREAGSRVLGMRHYDEQLIGGMVLHYGKIAEM
                     RTGEGKTLMATLPAYLNAISGKGVHVVTVNDYLASRDAEWMGRVYGFLGLSVGVILSQ
                     MPYDQKKSAYAADITYGTNNEFGFDYLRDNMASSANERFQRPLNYAVVDEVDSILIDE
                     ARTPLIISGQAEDNVDIYQHMDKLAPKLRRQADEDSEGDFSVDEKSHQILLSEVGHEH
                     AEELLAQAGMLPPGGSLYDPANITLIHLLYAALRAHNLYFRDQHYVVQDGEVVIVDEF
                     TGRLMAGRRWSDGLHQAVEAKEGVAIQKENQTLASITFQNYFRMYHKLAGMTGTADTE
                     AFEFQSIYGLETVIIPPHRPTIRKDMMDKVYLTAKEKHQAVIADIKECYERGQPVLVG
                     TTSIENSELLSELLEQEKLPHQVLNAKQHAREAEIVAEAGRPKMVTIATNMAGRGTDI
                     VLGGNVEKQIELLRADESLDDAAREQRVAQLKAEWQLVHEQVLQSGGLHIIGTERHES
                     RRVDNQLRGRSGRQGDPGSSRF"
     gene            complement(1841..2761)
                     /locus_tag="VFW59_08540"
     CDS             complement(1841..2761)
                     /locus_tag="VFW59_08540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5364306.1"
                     /translation="MNIILVSDRLAKSRSITLGGWQIALLAMSMFVMVWVATFALQYA
                     LARFAPESLNDGVRALLSKIQSEERQQQQTYVNSSLDAMASRVGQMQAQMQRLDALGA
                     RLAKLTGMKPDEFRFDQPPAQGGPLVMLPPEDMSVAGLQQQLATLTQLVSDRSDKLMA
                     LETMLMQNQLSRKLLPSIPPIAHGFYSSNFGWRIDPFTGKSAMHEGVDFMVPEGTAIR
                     ASAGGVVAFSDKHPDYGNMIEIDHGNDIVTRYAHASRLLVKVGQVVRRGEKIAEVGST
                     GRSTGNHLHFEVRYKGIAQNPVRFLKEAAG"
     gene            2794..3216
                     /locus_tag="VFW59_08545"
     CDS             2794..3216
                     /locus_tag="VFW59_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX5364307.1"
                     /translation="MAQPFRSLLGNAPELRPLLNKAHTLSALQRQFASVAPAYLGQSS
                     MVLGLQFGTLSIAVANSTVAAKLRQLAPELVVLLQNKGCEVSGIRVKVQVAYAAPQPQ
                     QRPRELSKTARNALNELGSNLDDSPLKQALQRMARKTG"
BASE COUNT          719 a         1138 c          922 g          709 t
ORIGIN      
        1 gaaacggctg gaaccggggt cgccctggcg tcccgaacgg ccgcgcaact gattgtcaac
       61 acggcgcgat tcgtggcgct cggtgccgat gatgtgcagg ccgccgctct gcagcacctg
      121 ctcgtgcacc agctgccact cggctttcag ctgcgcaacg cgctgctccc gggctgcgtc
      181 atccagactc tcgtcggcgc gcaacaattc gatctgtttt tcgacattgc cgcccagcac
      241 gatgtccgta ccgcgccccg ccatattggt ggcgatcgtg accatcttgg ggcgccctgc
      301 ctcggcgacg atctcggctt cacgcgcatg ctgcttggcg ttcagcacct gatgcggcag
      361 cttttcctgc tccagcaatt ccgacaacaa ctccgaattc tcgatcgaag tcgtgcccac
      421 cagcacgggc tgaccgcgct cgtaacattc cttgatgtcg gcgatcaccg cttggtgctt
      481 ttccttggca gtgagataca ccttgtccat catgtccttg cggatggtcg gacggtgcgg
      541 agggatgatc acggtttcca ggccatagat ggactggaat tcgaacgcct cggtatccgc
      601 cgtgccggtc atgcccgcca gcttgtgata catacggaaa tagttctgga aggtgatcga
      661 cgccagcgtc tgattttcct tctggatcgc gacgccctcc ttggcctcca ccgcctgatg
      721 caggccgtca gaccagcgcc gccccgccat cagacgaccg gtgaactcgt ccacgatcac
      781 cacttcgccg tcctgcacca catagtgctg atcgcggaaa tacagattgt gcgcacgcag
      841 cgccgcatac agaagatgaa tcaaggtgat attggcggga tcgtacaaac tgcctcccgg
      901 cggcagcatg ccggcctgag ccagcaattc ctcggcgtgc tcgtgcccga cctcggaaag
      961 caggatctga tggctcttct catccacact gaaatcgccc tcgctatcct catcggcctg
     1021 gcggcgcaat tttggcgcca gcttgtccat gtgctgatag atgtcgacgt tgtcttcggc
     1081 ctgaccggaa atgatcagcg gcgtacgcgc ctcgtcgatc aggatcgagt cgacctcgtc
     1141 caccacggcg tagttgagcg gacgctggaa gcgctcgttg gcgctggacg ccatgttgtc
     1201 gcgcaggtaa tcgaagccga attcgttgtt ggtaccgtaa gtgatgtcgg cggcgtaggc
     1261 ggattttttc tgatcgtaag gcatctgcga cagaatcacg cctaccgaca agccgaggaa
     1321 gccgtacacc cggcccatcc actccgcatc gcggctggcc agatagtcgt tcaccgtcac
     1381 cacatgcacg cccttgccgg agatcgcatt cagataggcc ggcagtgtcg ccatcagggt
     1441 cttgccctcg ccggtacgca tctcggcgat tttgccgtag tgcaacacca tgccgccgat
     1501 cagctgctcg tcgtaatggc gcatgcccag cacgcgactg cctgcctcgc gcaccaccgc
     1561 gaaggcttcc ggcaacagcg catccagcga ctcaccgctc gcaaagcgct gccggaaact
     1621 atccgtcttc tggcgcagtt cgtcgtcgct caacttcgca acgccagcct ccagtttgtt
     1681 gatggattga acggtggcgc gatactgctt gagcaggcga tcattacggc taccgaagat
     1741 acttttcaac gctctggaaa tcatgttttg ttttctctgc tgcaaaaaat aaaagggtga
     1801 ggcgatcaat cacctcaccc ccggacatac cacctcgcca tcaaccggcc gcttccttca
     1861 gaaaacgcac gggattctgg gcgatacctt tatagcgaac ttcaaagtgc agatggttac
     1921 cagtagagcg ccctgtgctg ccgacctcgg cgatcttttc gccacgccgc accacctgac
     1981 ccactttcac cagcaagcgc gaagcatgcg cataacgcgt cacaatgtca ttgccgtgat
     2041 caatctcgat catattacca tagtcaggat gcttgtccga aaaagccacg acaccgccag
     2101 ccgaggcgcg tatggcagtg ccttccggca ccatgaaatc gaccccttca tgcatcgcgc
     2161 tcttgccggt gaacgggtcg atgcgccagc caaaattcga ggaataaaac ccatgtgcaa
     2221 tcggcgggat agatggcagc aacttgcggc tcagctggtt ctgcatcaac atggtctcca
     2281 gcgccatcag tttgtcgctg cgatcgctca ccagctgcgt caaggtcgcg agctgctgct
     2341 gcaggcctgc caccgacata tcctctggtg gcaacatcac caacggaccg ccttgcgccg
     2401 gcggctgatc gaagcgaaac tcatccggct tcattcctgt cagcttggcc agacgcgcgc
     2461 ccagcgcatc cagacgctgc atttgcgcct gcatctggcc cacgcgcgac gccatcgcat
     2521 ccaggctgct attcacatag gtctgctgct gctggcgttc ctcgctctgt atcttggaca
     2581 acagtgcgcg cacgccatcg ttgaggcttt ccggagcgaa acgcgccaat gcgtattgca
     2641 gcgcaaaagt cgccacccag accatcacaa acatcgacat ggccagcagc gcgatttgcc
     2701 agccgcccag cgtgatgctg cgagattttg ccaaacgatc ggaaactaga ataatattca
     2761 tgcttccctc ccctatttat atttaaccac ctcgtggcac agccattccg ctcactgctc
     2821 ggcaacgctc cggaactccg ccctctgctg aacaaggctc ataccctgtc agcactgcag
     2881 cggcaattcg cgagtgtcgc acccgcctat ctgggtcagt ccagcatggt actggggctg
     2941 caattcggca ccctcagcat cgcggtcgcg aactccaccg ttgccgcaaa attgcgccaa
     3001 ctcgcgccgg aactcgttgt tttattgcag aacaagggct gcgaggttag cggaatacgc
     3061 gtcaaggtgc aagttgcgta cgccgctccc cagcctcagc aacgccctcg cgaactcagc
     3121 aaaaccgcac gcaacgccct gaacgaactc ggctccaatc tggacgactc gccgctcaag
     3181 caggcactgc agagaatggc gcgcaagacc ggctagatcg atgcgctccg gctggcacag
     3241 cagcccgaac acggcattaa cgcagccgga gtaaaaatag acaccagatc gacgcagata
     3301 ccgccgtttt gactgaagtt acgccgcttg cagctgcaac cgcagaaatg ccggcgcgtc
     3361 ttccacgcta tcgaaagtga cgaattccca agcctgttcg tccgccagca aggcgcgcac
     3421 cagcgcatta ttcagcgcat ggccggattt gaatcccgag aatgccccga tcaatgggta
     3481 ccccatca
//
LOCUS       DASUIV010000236         5277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_339183, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000236 DASUIV010000000
VERSION     DASUIV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339183"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFW59_08550"
     CDS             <1..196
                     /locus_tag="VFW59_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364308.1"
                     /translation="AMTYMAPDSPDVVEMIFSIAIRVIVVAAESMSKSAWFPEGVFEA
                     VFQDMNNAWGGAMNEADGVL"
     gene            complement(579..1346)
                     /gene="xth"
                     /locus_tag="VFW59_08555"
     CDS             complement(579..1346)
                     /gene="xth"
                     /locus_tag="VFW59_08555"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5364309.1"
                     /translation="MKLATWNVNSLKVRLPQVLEWLAANPVDALCLQETKQQDADFPQ
                     AELQEAGYHSVFIGQKTYNGVAILSREPAVDVQLGIPNFADEQRRVIAATLGDVRVVC
                     VYIPNGQSVDSDKYQYKLGWLAALQDWLKDELTRYPKLALLGDYNIAPEDADVHDPKA
                     WEGKVLVSEPERAAFKGLQALGLHDSFRLFEQPEKSFSWWDYRMMGFRRNLGLRIDHI
                     LISEPLVAQCKGCVIDKEPRRVERPSDHTPVVVELSV"
     gene            complement(1343..1876)
                     /locus_tag="VFW59_08560"
     CDS             complement(1343..1876)
                     /locus_tag="VFW59_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5364310.1"
                     /translation="MRRIFLLGCLMTLSLPVQAGDLYRWVDGKGKVQYGDAPPPNAAM
                     LEQLEFNDQAAANADLPYETRRAQKDFPVTLYTANDCGEICTQARALLTRRGIPFSDK
                     KLLTKEEIEAFKALSGSDIAPTLGVGKNFLKGFEAGQWNKELDIAGYPKTAPHPAPKA
                     APETPANAVAPAQPVTP"
     gene            complement(1902..3956)
                     /locus_tag="VFW59_08565"
     CDS             complement(1902..3956)
                     /locus_tag="VFW59_08565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5364311.1"
                     /translation="MNTPNLSANPLLDFTGLPRFAEIKPEHVAPAIEQLLDENRALIA
                     RLLADDAEPTWDNFVLPMEDANERLSRAWGPVGHLNAVMNSPELREVYNATLPVITQY
                     YAELGQNLALFGKFKALRNGKEYAGLSAARKKLLENELRDFRLGGAELPEDKKARYLE
                     IQERQSELSSRFSDNLLDATNDYTLVIEHRSELSGLPDDVLQAAQEAASAAGKQGWLF
                     TLKAPSYMPVMQYADNRALREKMYRASATRASEFGRAEWDNGPLMDEIVKLRAEEAVL
                     LGFANYGELSLAAKMADTPQQVVSFMRELAQRARPFAEKDLAELREFARDELNLSELH
                     SWDTTYASEKLREQRYAFSEQEVKQYFPEDAVLPGMFRLVETLYGLRIARAEAPLWHE
                     DARFYDIRDAKGDLVGQFYLDMYARASKRGGAWMDDVITRRRKAAGIQTPVAYLNCNF
                     PAPVGGKPATFSHDDVITLFHEFGHGLHHLLTEVEDMGVSGINGVEWDAVELPSQFME
                     NFCWEWDVVRGMTRQADTNEPLPRALFDKMLAAKNFQSGLQTLRQIEFSLFDMLMHSN
                     FEPGGNKSILQLLDEVRAEVAVLIPPDFNRFPHSFSHIFSGGYAAGYYSYKWAEVLSA
                     DAYSLFEEHGVLNPDVGARFRAEILAMGGSRGAMQSFAAFRGREPSIDALLRHNGLLE
                     QV"
     gene            complement(4131..>5277)
                     /locus_tag="VFW59_08570"
     CDS             complement(4131..>5277)
                     /locus_tag="VFW59_08570"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5364312.1"
                     /translation="HVIDADGLIVAPGLIDLAARLREPGYEYMATLESEMDAAVAGGI
                     TSLACPPDTDPPLDEPGLVEMLKHRARNLNQAHVYPVGALTAGLKGQELTEMAELVDA
                     GCVAFSQVDAPLTDTRVLMRAMQYAATFGYSVWLRPQDSFLAKNGVAHDGEVATRCGL
                     PAIPVSAETIALNTMLTLARDTGVKLHICRLSSGAAVEMIRAAKREGLPVTCDISMNH
                     AHLSEMDIGYFDPNCHLVPPLRSLRDRAALRAGLLDGTIDAICSNHAPVDEDAKQLPF
                     AEAEAGATGLELLLPLVLKWAQQENIALPAALAKATSQPATILGLDAGHLSVGAAADI
                     CVFDPDVYWKVEPAALKSQGKNTPFTGLEMQGKVRYTLVDGQVVYQA"
BASE COUNT          960 a         1694 c         1582 g         1041 t
ORIGIN      
        1 cgccatgacc tatatggctc ctgattcacc tgatgtcgtt gaaatgattt tttcaatagc
       61 gattagagtg attgttgttg cagccgaatc tatgtcaaaa agcgcttggt tccctgaagg
      121 ggtgtttgag gcggtgtttc aggatatgaa taatgcttgg ggtggggcaa tgaatgaggc
      181 ggatggagtt ctgtagagtg ggaacgaagt acccactccg tgtttttaga ttttgacttt
      241 ctttcccctg tgcgccgccg agtagcggag gacggtcggg ggtagtccga cgaatatgtt
      301 tgagcacgtg gccgcgcagc ggatcgtgcg agtttatgag gaggcccgac cgttcgagca
      361 acggagggaa cccacgaagt gggcggcaaa ccgggggcgc cttctttgga ttacctttct
      421 tggcaagaca agaaaggtaa tcagctgccg ggctgccccc ggcgaagttg atttgttttt
      481 gtcattcacc cttcgacaag ctgatgaaac aactagccat tcgactaggc agccaaacaa
      541 cgaccgccaa gtcgctggtt atcagggcga acggccaatt agaccgacaa ttccaccacc
      601 accggcgtgt gatccgatgg gcgctccacc cggcgcggtt ccttgtcgat cacacagccc
      661 ttgcattgcg ccaccagcgg ctcgctgatc aggatgtggt cgatgcgcaa tcctaaatta
      721 cggcgaaagc ccatcatgcg gtagtcccac caggagaaac tcttctccgg ctgctcgaac
      781 aggcggaagc tgtcgtgcag gccgagtgcc tgcagcccct tgaacgcggc gcgctccggt
      841 tcgctgacca gcaccttgcc ctcccatgcc ttggggtcgt gcacgtcggc atcctctggc
      901 gcgatgttgt agtcgcccag taacgccagt ttcggatagc gcgtcagctc gtccttcagc
      961 cagtcctgca gcgcggccag ccagcccagc ttgtactggt atttgtcgga atccacgctc
     1021 tgcccgttgg gtatgtagac gcacaccacc cgcacatcgc cgagggtggc ggcgatcacg
     1081 cgcctctgtt cgtcggcgaa attcgggatg ccgagctgca catcgacggc cggttcgcga
     1141 ctcaggatcg ccacgccgtt gtaggtcttc tgcccgataa acaccgagtg gtagcccgcc
     1201 tcctgcaact cggcctgcgg aaagtcggcg tcctgctgct tggtctcctg cagacacagc
     1261 gcatcgaccg gattggccgc gagccattcc agcacctgtg gcaggcgcac cttcagcgag
     1321 ttgacattcc aggtcgccag cttcatgggg tcacgggctg ggccggggcg accgcattcg
     1381 caggcgtttc cggggccgct ttcggcgcag ggtgtggcgc agtcttggga taaccggcga
     1441 tgtcgagctc cttgttccat tgcccggcct cgaaaccctt caggaaattc ttgcccacgc
     1501 cgagcgtggg cgcgatgtcg ctgccgctca acgccttgaa cgcttctatc tcttccttgg
     1561 tgagcaactt cttgtcgctg aaagggatgc cgcgccgggt cagcagcgcg cgcgcctgag
     1621 tacaaatctc gccgcaatcg ttcgccgtat acagcgtcac cgggaaatcc ttctgcgcac
     1681 ggcgcgtctc ataaggcagg tcggcattcg cggcggcctg atcgttgaat tccagctgtt
     1741 ccagcatcgc cgcgttcggc ggcggcgcat cgccgtattg caccttacct ttgccatcca
     1801 cccagcgata caggtcgccc gcctgtaccg gcagcgataa cgtcatcaaa caacccaaca
     1861 aaaagatgcg cctcattttt ccctcccgtc taacgaacca atcagacttg ctccagcagc
     1921 ccattatgcc gcaacagcgc gtcgatgctc ggctcgcgac cccggaaggc ggcgaacgat
     1981 tgcatcgcac cgcgactgcc gcccatcgcg aggatctcgg cacggaagcg cgcgcccacg
     2041 tccgggttca gcacgccgtg ttcctcgaac aggctgtagg catcggcgga cagcacctcg
     2101 gcccacttgt agctgtagta gcccgccgca tagccgcccg agaagatatg cgagaagctg
     2161 tgcgggaagc ggttgaaatc gggcgggatc agcaccgcga cttccgcgcg cacctcgtcc
     2221 agcaattgca agatgctctt gttgccgccc ggctcgaagt tgctgtgcat cagcatgtcg
     2281 aacagcgaga actcgatctg gcgcagcgtc tgcaggccgc tctggaagtt cttcgcggcc
     2341 agcatcttgt cgaacagcgc gcgcggcagc ggctcgttgg tgtccgcctg acgggtcatg
     2401 ccgcgcacga cgtcccactc ccagcagaag ttctccatga actggctggg cagctcgacc
     2461 gcgtcccatt ccacgccgtt gataccggac acgcccatgt cttccacctc ggtcagcagg
     2521 tgatgcaggc cgtgaccgaa ctcgtggaac agcgtgatca cgtcatcgtg agaaaaggtt
     2581 gcgggcttgc cgccgacggg tgccgggaag ttgcagttca gataggcgac cggagtctgg
     2641 atgccggctg ccttgcggcg gcgcgtgatc acgtcgtcca tccacgcccc gccgcgcttg
     2701 ctcgcccgcg catacatgtc gagatagaac tggccgacca gatcgccttt cgcatcgcgg
     2761 atgtcgtaga agcgcgcatc ctcatgccac agcggcgcct cagcccgcgc gatccgcagg
     2821 ccgtacaagg tctcgaccag acggaacatg cccggcagca ccgcatcctc gggaaaatac
     2881 tgcttcacct cctgctcgga gaaggcgtag cgctgctcgc gcaacttctc gctggcataa
     2941 gtcgtatccc aggaatgcag ctcgctcaga ttcaactcat cgcgggcgaa ctcgcgcagc
     3001 tcggccagat ccttctcggc gaagggccgc gcgcgctgcg ccagctcgcg catgaaagac
     3061 actacctgct gcggcgtgtc cgccatcttc gccgccagcg acagttcgcc gtaattggcg
     3121 aagcccagca gcaccgcctc ttcggcgcgc aacttgacga tctcgtccat cagcggacca
     3181 ttgtcccatt ctgcccggcc gaactccgag gcgcgggtgg ccgaggcacg gtacatcttt
     3241 tcgcgcagcg cgcgattgtc ggcgtattgc atcaccggca tgtaggacgg cgccttcagc
     3301 gtgaacagcc agccttgttt acctgccgca ctcgcggctt cctgcgccgc ctgcaacacg
     3361 tcgtccggca agccgctcaa ctcggacctg tgttcgatga ccagcgtgta atcgttggtc
     3421 gcatccagca ggttgtcgga gaaacgcgac gacaattcgg actggcgttc ctgtatctcc
     3481 agataacgcg ccttcttgtc ttccggcagc tcagcgccgc ccagccggaa gtcgcgcagc
     3541 tcgttttcca gcagcttctt gcgcgccgca ctcagccccg catattcctt gccgttgcgc
     3601 agcgccttga acttgccgaa cagcgcgagg ttctgcccca gctcggcgta atactgcgtg
     3661 atcaccggca gcgtggcgtt gtacacctcg cgcaactccg gcgaattcat caccgcgttc
     3721 aggtgcccca ccggccccca ggcgcgcgac agcctctcgt tcgcgtcctc catcggcagc
     3781 acgaagttgt cccaggtcgg ctctgcatcg tcggccagca gccgcgcgat cagcgcgcgg
     3841 ttctcgtcca gcagctgctc tatcgccggt gcgacatgtt cgggcttgat ctccgcgaaa
     3901 cgcggcagac cggtaaaatc cagcaaagga ttagcggata aattgggtgt attcataatt
     3961 ttttcgctat ataaaactgg ctggatttcg gtgcagacta acctgcttgc aggttatgtc
     4021 gtaatcaaaa tccagcaaag gattatctga agtatttgat gggttcatga ttggtcgtct
     4081 atgtaaggca ggatccccgc ctgagcgggg atgacgtaat tcagcgggtg ctaggcttga
     4141 taaaccacct gaccgtccac cagggtgtag cgcaccttgc cctgcatctc cagcccggtg
     4201 aacggggtgt tcttgccctg actcttcaac gcggcaggtt cgactttcca gtaaacatcg
     4261 ggatcgaaaa cgcagatgtc ggcggcggcg ccgacgctca ggtgaccggc gtccagaccg
     4321 aggatggtcg cgggctgcga ggtggccttg gcgagtgcgg cggggagcgc gatgttctcc
     4381 tgctgcgccc atttcagcac cagcggcagc aacagttcca gcccggtcgc tccggcttcc
     4441 gcctcggcga acggcagctg cttggcatcc tcgtccaccg gcgcatggtt ggagcagatc
     4501 gcatcgatgg tgccgtccag cagaccggcg cgcagcgcgg cgcggtcgcg caggctgcgc
     4561 agcggcggca ccagatggca gttcggatcg aagtagccga tgtccatctc ggacagatgg
     4621 gcgtggttca tcgagatatc gcaggtgacc ggcaggcctt cacgcttggc cgcgcggatc
     4681 atttccacag cggctccgct ggaaagacgg cagatatgca gcttcacgcc ggtgtcgcgc
     4741 gccagcgtca gcatggtgtt gagcgcgatg gtctcggcac tgaccggaat cgcgggcaag
     4801 ccgcagcgcg tggccacttc gccgtcgtgc gccacgccgt tcttggcgag aaagctgtcc
     4861 tgcgggcgca gccagacgct gtagccgaag gtcgcagcgt attgcatcgc gcgcatcagc
     4921 acgcgggtat cggtcagcgg cgcatcgacc tgactgaagg ccacgcagcc cgcatccacc
     4981 agctcggcca tctcggtcag ttcctgcccc ttcagaccgg cggtcagcgc gccgaccgga
     5041 tagacatgcg cctggttcag gttgcgcgcg cggtgcttga gcatctccac cagccccggc
     5101 tcgtccagcg gcggatcggt gtccggcggg caagccagac tggtgatgcc gcccgccacc
     5161 gccgcatcca tctcggactc cagcgtcgcc atgtattcgt agcccggctc gcgcaaccgc
     5221 gcggccagat cgatcagacc gggcgcgacg atcaggccgt cggcatcgat cacgtga
//
LOCUS       DASUIV010000237        16740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_339512, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000237 DASUIV010000000
VERSION     DASUIV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 16740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16740
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339512"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="VFW59_08575"
     CDS             <1..1880
                     /locus_tag="VFW59_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX5364313.1"
                     /translation="KHLERIKEGQTRFDAILTACKEVGPSLFFSLLIITVSFLPVFTL
                     EAQEGRLFAPLAFTKTFAMAGAALLSVTLVPVLMLLFVRGKIMPEAQNPLNRWLIKGY
                     RPLIHAVLQRKRATLAVAVLACIVSLYPAMKLGSEFMPTLNEGTLFYMPASLPGMSVT
                     KAAELLQTQDKIIRSFPEVASVYGKAGRAGTATDPAPMEMFETVINLKPQEDWRPGMD
                     TDKLIAEMDRALQFPGVANSWTMPIKARIDMLSTGIRTPIGIKVYGKNLDEMERLARE
                     IEAVVQKIPGTTSAFAERITGGFYLNIEPDRLALARYGLTMNEVQDVIATALGGENVT
                     TTVEGLERFGVNLRYPRELRSTPEQIAREVLVPTMDGAMIPLGQLARISLSKGAPSIR
                     TENALLSAYIYVDIRDRDIGSYVAEARRAVAAQVKFPPGYYATWSGQFEYMERAAARM
                     KIVIPITLLLIFLLLYLNFRRVTESLIVMLSVPFALVGGVWLLWLLDYNLSVAVAVGF
                     IALAGVAAETGVVMLIYLEQAWRHIQSRCADEGRKPTVADLHAAIMEGAVERVRPKMM
                     TVVAIMAGLLPILWSSGTGSEVMRRIAAPMVGGMLSSAVLTLLVVPVLYALAKAREIK
                     S"
     gene            1932..4355
                     /locus_tag="VFW59_08580"
     CDS             1932..4355
                     /locus_tag="VFW59_08580"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5364314.1"
                     /translation="MTPDIDPVCGMTVAEDKPHHLEHRGRSYRFCSKKCLDKFQANPD
                     AYLHKASAAVQPPPVTGSYTCPMHPEVIRTEPGACPICGMALEPRNAIVEDSAELNDM
                     TRRFRVSAVLVLPVFVLAMVSDLAPQFLPDSVSMTALQWLEFVLATPAVLWGGWPIFQ
                     RGWTSLLNRSLNMFTLIALGVGVAWSYSVAAMLLPGSFPSAMHSMGETVPVYFEAASV
                     IMALVLLGQVMELRARSQTSAAIRLLLGLVPKTARIVRSPLRNPFPLIPSPQSSPASG
                     RGSEREIQSLTPAGEGAIESLREFLHNSNEEDIPLDQVQAGDVLRVRPGEKVPVDGVV
                     LEGTSALDESMVTGESIPVEKTAGARLIGATVNGTGSLLMRAERVGADTLLAQIVHMV
                     SEAQRSRAPIQRLADVTAGYFVPAVIGVALATLAVWGVFGPEPRLAHAVVNAVAVLII
                     ACPCALGLATPMSIMVGTGRGATLGVLIKNAEALETMEKVNTLVVDKTGTLTEGRPKL
                     ASVTPLAGFEEGVVLWLAASLERASEHPLAAAIVKGAQERDITLKAVSEFRSVTGKGV
                     TGTVEGRQVALGNLKLFEQLHIDAGELPARAEALRSDGQTVMLLAIDGIAAGLIGVAD
                     PVKESTAEAIRALHEEGVEVIMLTGDNRVTAEAVAKKLGIARVEAEVLPEQKAGIVKR
                     LQAQGRIVAMAGDGINDAPALAQAQVGLAMGTGTDVAMESAGITLIKGDLNGIVRALR
                     LSRATMKNIRQNLFFAFVYNALGIPVAAGLLYPFFGLLLSPIIAAAAMSFSSVSVIGN
                     ALRLRRIKL"
     gene            4639..6114
                     /locus_tag="VFW59_08585"
     CDS             4639..6114
                     /locus_tag="VFW59_08585"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5364315.1"
                     /translation="MLIGKAVSIFTHDGDVMDVRARIVFGFSVPVLMFFAFGLWVALV
                     MQGASNHLRNVREESIALALMAKDMGMNVAQVQQYLTDISATRGQGGLNEGFKDAERH
                     YNNFNTELAKFEQFFASRGDQKRVKECRLIRRSFDNFYANGLKMAQAYIDGGPSLGNR
                     QMLDFDRTSRVLQGALTPFIKAQLGEMDVAIAQAERDAKTVRVVGLVLGLLAAIISIL
                     VARATVHTFSQYLAKRKQAESALKQARAELNEKNLILMDEKELLEDIVTNMRSGNQLD
                     GATIRCVQSSLERTSGDIVLSAFRPDGAQHVLVGDFSGHGLQASFGNLLVPYIFNRLT
                     AEGCSMRLLLEEVNRVLYRQLPSQLYMAACALELSPDRTQVLVWNCGMPPVLGVTAAQ
                     GINPINSAGLPLGISESIDSFAAHAQIQAEPAMRIYLYSDGITEASSSESGLYGQAQL
                     EALVLRLYREQLPLDVIWQELENYCGGQGLSDDAVMVEISP"
     gene            6314..8380
                     /locus_tag="VFW59_08590"
     CDS             6314..8380
                     /locus_tag="VFW59_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX5364316.1"
                     /translation="MQNKNAYVSQKEIPFPPKAVLISRTDTKGIMTYANDAFVEISGF
                     SREELIGKSHNIVRHPDMPPQAFKSLWDAMKERRPWRGTVKNRCKNGDHYWVRATVAP
                     VFENGEVVGYSSVRRPPTREHVAAAEGLYRRLNQSGDQIESRFERYKFGNWSLSRKLQ
                     FAIQVTLLIALSFGQYSVFEHMKEDARAAAISDAKKLSNEIIDSANMLMVAGQISNPD
                     LRQLLMKKISVAEGVRMAQLVRAKPVVDLYGPGLPEEKLANDVQRQAIESKQQIVAFN
                     EDGTHVHVVTPYLASKDFHGTDCTGCHQAEVGTVLGVSELEIDVTPQFESIATLEKRT
                     LAGQIGLQVFLFFYLAFLVKKYVATPAEVAQREFEKLMQGDMNEEIDISGRDELGVLL
                     CGVQTMQSYLRTIVDEVVTPVGKMQTRIQDMGSRVADVADNAANERQHIQQITGTMDG
                     FSRSVAGVADMAGDSLNDAKAMQRVVEENNRNMELSIAATSKVADTVQSTSRTISDLG
                     TAVNNIGSIANAIKEIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTAAS
                     TKDIATTISEISTISDAAVSSMHMAVTEVETGISLIRKNGDGLREILQATNSVSQRIE
                     HIAGASKEQSEAGISMSASLESISNLVDSNAQAAIEAKLEAEELAKSAVELKKAGYPL
                     TKCAIG"
     gene            8444..8764
                     /locus_tag="VFW59_08595"
     CDS             8444..8764
                     /locus_tag="VFW59_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364317.1"
                     /translation="MKFVPSLPPPRITGVGGGLKVNRLTAVGAVRPVQPRTLPPLVLL
                     PHEVHEPPEVERRHDRPEQVERRKYCRRVQHRPVLVEFRSGLDRRHRSQREGDPTEHV
                     DIEV"
     gene            8900..9769
                     /locus_tag="VFW59_08600"
     CDS             8900..9769
                     /locus_tag="VFW59_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HEX5364318.1"
                     /translation="MKSALWDKKALLGSPLFFPLHPVIAPLAEEGFPSMQDCNDLLLS
                     HQPPITVRNGLPLRFVAQEYGKLEFEAQYEPRCYLKGEVQTRADNWHDLLNALVWLTF
                     PKAKAVINMRHYQALTQAGQDSERMLMGDAVERSQRGAVRDMSTLFDESGVVVACAEE
                     ELGQLLCDFRWRELFWQQRERVRARMGFFLFGHGLYEKALHPYVGVTGQGLLIPVEAA
                     FFGWPLERQLTHLDELLAAYLAAPAHCRSTAELTPVPLLGVPGWAAENAEETYYDNAA
                     YFRPGRRKVKAMR"
     gene            complement(9766..12420)
                     /locus_tag="VFW59_08605"
     CDS             complement(9766..12420)
                     /locus_tag="VFW59_08605"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX5364319.1"
                     /translation="MNPTPDDANPEAEHPLPKAVRALPRMRPILLLPLLVLILGLLAT
                     QLIWSDSQRQLQTELQNEFDYGIHRTEFNIGQRLSDYEDVLYGLQALYSASDEVSRSE
                     FSAYVAGLHLDEHHPGIQSVAFSISVSGAQKEQHLAAIRKQGFPDYTIHPEGIRDLYV
                     PAIYVEPFQGENLREFGLDNYATAERRATLERARDSEQISVSGKLTLAQGVDAQQTSI
                     LMFMPVYRNGVAHNTAESRRANLVGWVSAAFRIQDLAAQLIGASSRNINIDIYDGTEM
                     TAETLLYSSENARDTSAAFKSVRQIQIADRLWTLSFSSKPAFDAKLLHSRTGTLAISG
                     IVVSLILSLFTWLMLYSRERALLDSARLERAKRLAERANQAKSEFLANMSHELRTPMN
                     GILGMTHLALRNETDSHKLNLLEKIQFSGEHLLHTIDNVLDLSSLESGRLHLNPFDFM
                     LREILSDLDGQLTPKASHKGLELAFDIPPELYALKLHGDAARLEQVLLNYACNAIKFT
                     EQGRIVIRARILEEDERSCKLRFEVEDTGIGIDMDKRPPLFTPFWQVDASSSRTHEGS
                     GLGLAICSELVSMMEDGQVGVESALGKGSTFWFSARFDKTGSSVPAGKERKISPELFA
                     NFNGARILVADDNSLSQMVMGELLKSAGASVQSAWSGREALELLQQNRYDCVLMDVQM
                     PLMDGIETTQAIRSDPAWAKLPIIAVTAHASDENRQRCLQAGMNDFMGKPFTPSAFYA
                     TLARWLPIPAVQEATRDAPAPSRQDSTIWSGDSALIDLSMLADLIGDDRTKMREFATK
                     FLTLARADMTQIESALERNDLDMLGKLAHHNKAPARMVGALGFANLCQQLVDHSKYAS
                     TPDQIRETIIQMRPLLDRIEQYIQLKLA"
     gene            complement(12435..13067)
                     /locus_tag="VFW59_08610"
     CDS             complement(12435..13067)
                     /locus_tag="VFW59_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5364320.1"
                     /translation="MIRLMIADDHELVRAGLSQYLDLSPDVKVIAEASDGTTLLEKLR
                     STPVDLVLLDLNMPGLSDADLIMRLRSAYPDLLILVLSMHNEVQTVLRAMKAGASGFI
                     SKNCTPPALLNAIRKVSATGKYLPPEIAEQLAFASTGPHSDSSDPLAMLSQREQQIFR
                     LLVTGVAINEIAEQLCISDKTVSTHKSNLLNKLGLKNIAELVHFAVSHRL"
     gene            complement(13134..14468)
                     /gene="hpnE"
                     /locus_tag="VFW59_08615"
     CDS             complement(13134..14468)
                     /gene="hpnE"
                     /locus_tag="VFW59_08615"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEX5364321.1"
                     /translation="MINPAIIGGGYAGMAAAVALAEQGIPVTVFESAKQLGGRARGVM
                     HNGTQLDNGQHLLLGCYRDTLRLIEQVGGDLEQDFLHLPLQLDLHGQFSLRAPHLPAP
                     LHLLIALLGAQGLSWRERIKAARFMLALRRMDFHLSSDMTVAELLARYGQDADLTLKL
                     WEPLCIAALNTPIRKASAQVLLNVLRDALNQSRADSDMLLPRLDFTALFPQRAATYVE
                     QRGGKVQLSCGVESIGPQHDGFELVTEAGPQHFSHVICAAPPVVAAKLLRPIPALANA
                     VQQIDALEHQPIYTVYVQYPADVTLPHPMLGLHQRFSQWLFDKGKIGGQHGLIAAVIS
                     AEGIHQDLSQEALAGKVVKELLEEFGISRQPAWFKVIAEKRATFCCAPDLQRPVQHTA
                     LSRLWLAGDYTAGDYPATLEGAVQSGLRCAARLRQQCRPDDEKPQPNLGIRL"
     gene            complement(14551..15384)
                     /gene="hpnD"
                     /locus_tag="VFW59_08620"
     CDS             complement(14551..15384)
                     /gene="hpnD"
                     /locus_tag="VFW59_08620"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX5364322.1"
                     /translation="MTPDQYCQDKAAASGSSFYNSFRFLPQDKRRAITALYAFCREVD
                     DVVDECSDENVARTTLNWWRNEVVAIYGGTPQHPVAQALVPIVKQFNLAQEHLLEIID
                     GMEMDLEQARYADFKSLQLYCYRVASVVGLLTVEIFGYTDRETLKYAHDLGIAFQLTN
                     IIRDVGEDARRNRIYLPMDELQQFGVTAADILNANETENFQRLMAFQIERAQRYYRQA
                     LDHLPAVDRKSQRTGLIMAAIYSATLDEVVATGCHVLKGRVSLTPTYKLWLAFKAWFQ
                     N"
     gene            complement(15396..16208)
                     /gene="hpnC"
                     /locus_tag="VFW59_08625"
     CDS             complement(15396..16208)
                     /gene="hpnC"
                     /locus_tag="VFW59_08625"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX5364323.1"
                     /translation="MSSVEHYENFPVASVLMPKRLRRPVAAIYHFARAADDIADEGEL
                     PDEERRRRLDEFRTELKRIETDEEPLTPLFRNLAAEVKQYALPMQPLYDLLDAFSQDV
                     VKKRYADFDELLDYCRRSANPVGNLLLHLYEEATPVNLAYSDAICSALQLINFWQDVA
                     KDWAIGRVYLPQDDLARFGVSEAQIAQGMTDASWHALMQFEVQRAREMMLAGRPLGTI
                     LTGRIGLEMRMIIAGGLRILDKLEAADYDMFNKRPVLRPFDWVIMLAKSAPL"
     gene            complement(16208..>16740)
                     /gene="prmB"
                     /locus_tag="VFW59_08630"
     CDS             complement(16208..>16740)
                     /gene="prmB"
                     /locus_tag="VFW59_08630"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5364324.1"
                     /translation="SPWIAEPEEIGSVLDMCTGSGCLAILAAHAFPNASVDAVDLSPD
                     ALAVAARNVSDYGLNEQIELIESDLFTNLGGRKYDLIISNPPYVNAESVAALPKEYLH
                     EPKLSLGSGEDGLDATRVILAQAAEHLTDNGILIVEIGHNRDALEAAYPNLPFTWLEI
                     SAGDEHVFMLHRNDLL"
BASE COUNT         3178 a         4623 c         5455 g         3484 t
ORIGIN      
        1 acaagcatct ggagcgcatc aaggaaggcc agacgcgctt cgatgcgata ttgacggcct
       61 gcaaggaagt cgggccgtca ctgttcttct cgttgctgat catcactgtg tccttcctgc
      121 cagtgttcac gctggaagcg caggaagggc gcttgtttgc accgctcgcg ttcaccaaga
      181 ccttcgctat ggcgggcgcg gccttgctgt cggtcacgct ggtgccggtg ctgatgctgc
      241 tgttcgtgcg cggcaagatc atgccggagg cgcagaatcc gctgaaccgc tggctgatca
      301 agggctatcg gccgctgatc catgcggtgc tgcaacgcaa gcgggccacc ctcgccgtcg
      361 ccgtactggc ctgcatcgtg agtctgtatc ctgcgatgaa gctgggcagc gaattcatgc
      421 ccacgctgaa cgagggcacg ctgttctaca tgcccgcgtc gttgcccggc atgtcggtga
      481 ccaaggcggc cgagctgttg cagacgcagg acaagatcat caggagcttc cccgaggtgg
      541 cgtccgtgta cggcaaggca gggcgcgcgg gcacggcgac cgatccggct ccgatggaga
      601 tgttcgagac ggtgatcaac ctcaagccgc aggaggactg gcgccccggc atggacaccg
      661 acaagctgat cgcagagatg gaccgtgcgt tgcagttccc cggcgtggcg aactcgtgga
      721 ccatgccgat caaggcgcgc atcgacatgc tctccaccgg catccgcacg ccgatcggca
      781 tcaaggtgta cggcaagaat ctggacgaga tggagcgtct ggcccgcgag atcgaagcgg
      841 tggtccagaa gataccgggt acgaccagtg cgttcgccga gcgcatcacc ggcgggttct
      901 acctgaacat cgaaccggat cgtctggcgc tggcgcgcta cgggctgacg atgaacgagg
      961 tgcaggacgt gatcgccacc gcgctgggcg gcgagaatgt caccaccacg gtcgaaggtc
     1021 tggagcgctt cggcgtgaac ctgcgctatc cgcgcgagtt gcgcagcacg ccggagcaga
     1081 tcgcccgcga ggtgctggtg ccaaccatgg atggagcgat gatcccgttg gggcagctcg
     1141 cccgcatcag cctcagtaaa ggtgcgccct cgatacgcac cgagaacgcg ctgctttccg
     1201 cctacatcta cgtcgacatt cgcgaccgcg acatcggctc ctacgtggcc gaagcgcgtc
     1261 gtgcggtggc ggcacaggtg aagtttcctc ccggctacta cgccacttgg agcgggcagt
     1321 tcgaatacat ggaacgcgcc gcagcgagga tgaagatcgt gatccccatc acgctgctgc
     1381 tgatcttcct gctgctgtat ctgaatttca ggcgcgtcac cgagtcgctg atcgtgatgc
     1441 tgtctgtgcc cttcgcgctg gtgggcgggg tgtggctgct gtggctgctt gactacaacc
     1501 tgagcgtcgc ggtggcggtg ggattcatcg cgctcgccgg tgtcgcggca gagaccggcg
     1561 tggtgatgct gatctatctg gagcaggcct ggcggcatat ccagtcgcgt tgtgccgatg
     1621 aggggcgcaa gcccacggta gccgacctgc atgcggccat catggaaggc gcggtggagc
     1681 gtgtgcgtcc gaagatgatg acggtggtgg cgatcatggc cgggctgttg ccgatactgt
     1741 ggagcagcgg caccggctcg gaagtgatgc gccgcatcgc cgcgcccatg gtcggcggca
     1801 tgctctcttc ggcggtgctg acgctgctgg tggtgccggt gttgtatgcg ctggccaagg
     1861 cgcgcgaaat aaaatcctga tgcacgaggt gcagcagcga tgatagaaat gattcgaaaa
     1921 aggagcgcgc catgacacct gacatcgatc ccgtctgcgg aatgacggtt gccgaggaca
     1981 agccgcatca tctggagcac cgggggcgga gctatcgttt ctgctcgaaa aagtgtctgg
     2041 ataagtttca ggcgaaccct gatgcatatc ttcacaaggc ctcagctgct gttcaacctc
     2101 ctcccgttac cgggagttac acctgtccga tgcatcccga agtgatacga accgaaccgg
     2161 gagcctgccc gatctgcggc atggcgctgg agccgcgcaa tgcgatcgtt gaagatagcg
     2221 ccgaactgaa cgatatgacg cggcgcttcc gggtgagcgc ggtactggtc ttgccggtgt
     2281 ttgtgctggc gatggtttcc gatctcgccc cccaattcct gccagactcg gtttcgatga
     2341 ccgcgctgca atggctggag ttcgtgctgg ccaccccggc ggtgctgtgg ggcggctggc
     2401 cgatcttcca gcgcggctgg acatcgttgc tcaaccgcag cctcaacatg ttcacgctga
     2461 tcgcgctggg tgtcggtgta gcctggagct acagcgtggc agcgatgctg ttgccgggca
     2521 gcttcccgtc cgcgatgcac agtatgggcg agaccgtgcc ggtgtatttc gaggcggctt
     2581 cggtgatcat ggcgctggtg ctgctggggc aggtgatgga gttgcgcgcg cgcagccaga
     2641 ccagcgccgc gatcaggctg ctgctgggcc tcgtccccaa gactgcgcgc atcgtgcgat
     2701 cccctctccg caaccctttc ccgttaattc cctctcctca atcctctccc gcaagcgggc
     2761 gaggaagcga gcgtgaaatt caatcgttaa ctcctgcggg cgagggagcg atcgaatcgc
     2821 tacgcgaatt tctccataac agcaacgagg aagacattcc gctggatcag gtgcaagcag
     2881 gcgatgtgtt gcgcgtgcgc cccggcgaga aggtgccggt ggatggcgtg gtgctggaag
     2941 gcaccagcgc cttggatgaa tccatggtca ccggcgaatc catcccggtg gagaagacgg
     3001 cgggcgcgcg actgatcggg gccacggtga acggcaccgg cagtctgttg atgcgcgcgg
     3061 agcgcgtcgg cgccgacacg ttgctcgcgc agatcgtcca catggtgagc gaggcgcagc
     3121 gttcgcgcgc cccgatccag cggctagccg atgtcaccgc cggttatttc gtgccggcgg
     3181 tgataggcgt tgcactcgcc acgctggcgg tatggggagt ctttgggccg gagccgcgtc
     3241 tggcgcacgc cgtggtcaac gcggtggctg tgctgatcat cgcctgtcct tgcgcgctgg
     3301 ggctggctac gccgatgtcc atcatggtcg gtaccgggcg cggtgcgaca ctcggggtgc
     3361 tgatcaagaa tgccgaggcg cttgagacca tggagaaggt caacacgctg gtggtggata
     3421 agaccggcac gttgaccgaa gggaggccga agcttgcctc ggtcacgccg ctggccggat
     3481 ttgaagaggg ggtcgtgctg tggctggcgg ccagcctgga gcgcgccagc gaacatccgc
     3541 tggcggcggc gatcgtaaag ggggcgcagg aaagggatat cacgctcaaa gcggtcagcg
     3601 agttccgctc ggtcaccggc aagggcgtga cgggaacggt cgaggggcgt caggttgcgc
     3661 tgggcaatct caagctgttt gaacaattgc atatcgatgc cggcgagttg cctgcacgcg
     3721 ccgaggcatt gcgcagcgac ggccagaccg tgatgctgct ggcgatcgat ggcattgcgg
     3781 cggggctgat cggtgtggcc gacccggtca aggaatccac ggcggaggcg atacgcgcac
     3841 tgcacgagga gggtgtggag gtcatcatgc tcaccggcga caatcgcgtc accgctgagg
     3901 cggtggcgaa aaaactgggc atagcccggg tcgaggccga agtcttgccc gagcagaagg
     3961 cgggcatcgt caaacgactg caggcacagg gacgcatcgt ggcgatggcg ggcgacggca
     4021 tcaacgatgc gcccgcgctg gcacaggctc aggtcggtct cgcgatgggg accggcaccg
     4081 atgtggcgat ggaaagcgcg ggtatcacac tgataaaagg cgatctgaac ggcatcgtgc
     4141 gcgcgctgcg cctgtcccgt gccaccatga aaaatatccg ccagaacctg ttcttcgctt
     4201 ttgtctataa cgcgctgggt atcccggttg cggccgggtt gctgtatccg ttcttcggcc
     4261 tgctgctttc gcccatcatc gcggcggcgg cgatgagttt cagctcggtg tcggtgatcg
     4321 gcaatgcatt gcgattgcgt cgcatcaagc tttagccgaa gacgatcctt catggcgagg
     4381 ctgcagccgg tttcggggca tcgtgctggc ggttccggct tgatgggcag ttcgagctgc
     4441 atcgttcccg gcaacctcca ggtcaagacg aataaatcgt ggagaaccgt tgaatttgaa
     4501 cgagtgtaaa taatgccgat taggtattat gggtagactg tttttgcccc aagcaaaaaa
     4561 cggggccagt tagccatgag tggcgagcct gctcgttgca gagacgaagt tacatagggc
     4621 gttttatttt tagattggat gttgatcggc aaggctgtat cgatttttac gcatgacggt
     4681 gatgttatgg atgtacgcgc aagaatagtc tttgggtttt cagttcctgt cctgatgttt
     4741 tttgcattcg gactgtgggt tgctcttgtg atgcaggggg cgtccaatca tctgcgcaac
     4801 gtcagggagg agagtatcgc cttggccttg atggccaagg acatgggaat gaacgttgcc
     4861 caggtgcagc aatatctgac cgatatttcc gccacgcgcg ggcagggtgg gttgaacgaa
     4921 ggattcaagg atgccgagag gcactacaat aatttcaata ccgaacttgc aaagttcgaa
     4981 cagttcttcg catccagagg ggatcagaaa cgcgtcaagg agtgcaggct gatcaggagg
     5041 tctttcgata atttttatgc gaacggactc aagatggcgc aggcctatat cgatggcggg
     5101 ccttctctgg gaaatagaca gatgctggat ttcgacagga ccagccgggt cttgcagggg
     5161 gcgttgacgc cgttcatcaa ggcccagctg ggcgaaatgg acgtggctat cgcacaggcc
     5221 gagcgcgacg ccaaaacggt acgtgtcgtc gggctggtgc tgggcctgct ggccgcgatt
     5281 atttcgatcc ttgtggccag agcgacggtt cataccttca gtcagtacct tgccaaacgc
     5341 aagcaggccg agtctgccct gaaacaggcg cgcgcagagc tgaatgaaaa gaacctgatc
     5401 ctcatggacg aaaaggaatt gctggaggat atcgtgacca acatgcgttc gggaaaccag
     5461 ttggatggag ccacgatacg gtgtgtccag tcatcgctgg agcgaacctc gggcgatatc
     5521 gtgttgtctg ccttccgccc ggatggggcc cagcatgtgc tggtcgggga tttttccggt
     5581 cacggcttgc aggcctcgtt tggaaatctg ctggtcccgt atatttttaa ccgtttgact
     5641 gccgagggct gcagcatgcg cctccttctg gaggaggtga atcgtgtcct gtatcgccaa
     5701 ctcccttccc agctttatat ggcagcctgc gcactggagt tgtcgcctga ccggacgcag
     5761 gtgctggtct ggaattgtgg tatgccgcct gtgcttggtg tgactgctgc gcagggcatt
     5821 aacccgatca attcagcagg gctgccgttg ggtatcagtg aatcgataga cagctttgct
     5881 gcccatgcgc agatccaggc ggaacccgct atgcgcattt atctgtattc ggatggcatt
     5941 accgaggcga gctcgagtga atccggattg tatggacaag cacagctcga ggcgctggtc
     6001 ctgcgccttt accgggagca actgccgctc gatgtgattt ggcaggaact ggagaactac
     6061 tgcggcgggc aaggattgtc ggacgatgcg gtcatggtcg agatttcacc ttaacggaat
     6121 cttgcgaaaa agagggaacc ccatggcttg ccgaatgttt aatgggccct tttccctatg
     6181 ctgtttaagg ttttgcccgg cgcacgggat tgttaatatc atccgagcaa tagtacaggt
     6241 tgacgatgct cgtcaattcc atatcgacaa ggccgaacaa taattagatg cggtcgatca
     6301 ggggggaata gttgtgcaga acaagaatgc ctacgtttca caaaaggaaa taccgtttcc
     6361 accgaaggcc gtactgatat ccagaaccga taccaagggc atcatgactt atgccaatga
     6421 tgctttcgtg gaaatctccg ggttcagtcg tgaagagttg atcggcaaga gtcacaacat
     6481 cgtgcgtcat ccggacatgc cgccgcaggc attcaagtcg ttgtgggatg cgatgaagga
     6541 gcgacgtccc tggcgcggta cggtgaagaa tcgttgcaag aacggggatc attactgggt
     6601 gcgcgccacg gtcgcaccgg tcttcgagaa tggcgaggtc gtcggttatt cctcggtgcg
     6661 ccgtccgccg acccgtgaac atgttgcagc ggccgagggc ttgtatcgcc gcttgaatca
     6721 gagtggtgat cagatcgagt cgagattcga acgctacaaa ttcgggaatt ggtcgctgtc
     6781 gcgcaagctg cagttcgcca ttcaggtcac gctgctgatc gccctgagtt tcgggcagta
     6841 ttccgtgttc gagcatatga aggaagatgc cagggcagcg gccatcagcg atgccaagaa
     6901 actctccaac gagatcatcg atagcgccaa catgttgatg gttgcagggc agatcagtaa
     6961 tcccgatctg cgccagttgc tgatgaaaaa gatcagtgtg gccgagggtg tccggatggc
     7021 gcagttggta cgcgcaaagc cggtggtgga tctgtatggc cccggcctgc cggaggagaa
     7081 gctggcgaac gatgtgcagc gtcaggcgat cgaatccaaa cagcagatcg tggcgttcaa
     7141 tgaggacggc acgcatgttc atgtcgtcac gccttatctg gccagcaagg attttcacgg
     7201 cacggattgc accggttgcc atcaggctga agtcgggacg gtactcgggg tctccgagct
     7261 ggagatcgat gtgacgccgc agttcgagtc gattgccacg ctcgagaaac ggaccctggc
     7321 aggacagatc ggcttgcagg tgttcctgtt cttctatctg gcttttctgg tcaaaaaata
     7381 tgtcgccacg cccgccgagg tcgcgcagcg cgagttcgag aagctgatgc agggcgatat
     7441 gaacgaggag atcgatatct ccggccgcga cgaactgggc gtgttgttgt gcggcgtgca
     7501 gaccatgcag tcctatttgc gcaccatcgt cgacgaggtg gtcacgccgg tcgggaagat
     7561 gcagacacgc atacaggaca tgggcagccg ggtggcggac gtggcggaca acgcggcgaa
     7621 cgagcgtcag cacatccagc agatcaccgg cacgatggat ggcttcagcc gctcggtggc
     7681 cggggtcgcg gacatggccg gagattcgct gaacgatgcc aaggcgatgc agcgggtggt
     7741 cgaggagaac aatcgcaaca tggagctcag catcgcggcg accagcaaag tggccgatac
     7801 cgtgcagagc accagcagga cgatttccga tctgggtacg gccgtgaata acatcggttc
     7861 cattgccaat gccatcaagg agatcgccga gcagaccaac ctgctggcgc tgaatgcggc
     7921 catcgaggcg gcacgtgccg gtgagcaggg acgcggtttt gccgtggtcg cggacgaggt
     7981 gcgcaagctg gcggaacgta ctgcggccag taccaaggat atcgcgacca cgatcagcga
     8041 gatcagtacg atttcggatg cggcggtgag ctccatgcat atggccgtga ccgaagtgga
     8101 gacgggcatc tccctgatac ggaagaatgg cgatggcctg cgggaaatac tgcaggcgac
     8161 caatagcgtc tcgcagcgca tcgagcatat cgcgggcgcc tctaaagaac agtctgaggc
     8221 gggtatcagt atgtcggcca gcctggagag catcagcaat ctggtggata gcaatgcaca
     8281 ggcggccatc gaggccaagc tcgaggccga ggagttggca aaatcggcgg tcgagttgaa
     8341 gaaggccggt tatccgctga ccaagtgcgc catcggctga gccgttcgct ctggtgtcgg
     8401 cgggcgtttc tgatcgttcg cgtatttcat ccgggaggcc agcatgaaat tcgtgcccag
     8461 ccttcccccc ccgagaataa ccggggtggg agggggattg aaggtgaaca ggctgactgc
     8521 ggtcggggcg gtaaggcccg ttcagccgcg cactctgccg ccgttggtgc tgctgccgca
     8581 tgaggtgcat gaaccacccg aagtcgagcg gcgacacgat cggccagagc aggttgagcg
     8641 gcggaaatat tgtcggcgcg tccagcatcg gcccgttctg gtggagttcc gttccgggct
     8701 ggatcgtcgc catcgctcac agagggaagg cgatccgacc gagcatgtcg atatcgaagt
     8761 ttaatgcctg cgttttgggt aaggggggct gccagacttg tccctcaaca atattgagtc
     8821 cgatgtactg gactgctaac gttacataat ttttcgtgaa gtactgcgtg cgttacatca
     8881 ccgaataatc cgccgacaca tgaaatcagc tctctgggac aagaaggcgc tgctggggtc
     8941 tccgttgttc tttccgttac acccggtcat cgcaccgctt gccgaagagg gatttccctc
     9001 gatgcaggac tgtaatgact tgctgctgag tcaccagcct cccatcaccg tacggaatgg
     9061 gctgccgttg cgtttcgtgg cgcaggaata tggcaagttg gaattcgagg cgcagtatga
     9121 gccgcgttgc tatctcaagg gtgaggtgca gacccgcgcc gacaactggc atgacctgct
     9181 caatgctctg gtatggctga ccttccccaa ggccaaggcc gtcatcaata tgcgtcatta
     9241 tcaggcgctg acccaggccg gtcaggactc ggagcggatg ctcatgggag atgcggtgga
     9301 gcgcagtcag cgcggagcgg tgcgcgacat gagtacgctg ttcgacgagt ccggtgtggt
     9361 cgtggcctgt gccgaagagg agcttgggca gttgttatgc gattttcgct ggcgcgagct
     9421 gttctggcag cagcgcgagc gagtgcgggc gcgcatggga ttctttctgt tcggtcacgg
     9481 cctgtatgaa aaggcgctgc acccttatgt cggggtcacc ggccaaggcc tgctgatacc
     9541 ggtcgaggcg gcattctttg gctggccgct ggagcggcaa ctgacccatc tggacgaatt
     9601 gctggcggcg tatctggcag cgccggctca ttgccgcagc acagcggaac tgaccccggt
     9661 accgctgctg ggtgtgcccg gttgggcggc ggagaatgcc gaggaaactt attacgacaa
     9721 tgccgcgtat ttccgaccgg gcagacgcaa ggtcaaagcg atgcgctaag ccagcttgag
     9781 ctgtatgtac tgttcgatgc gatccagcag cgggcgcatc tgaatgatgg tttccctgat
     9841 ttgatccggg gtgctggcat acttgctgtg atccaccagt tgctgacaca gattggcaaa
     9901 acccagcgca ccgaccatcc tcgcgggcgc cttgttgtga tgcgccagct tgcccagcat
     9961 gtcgagatcg tttcgctcca gcgccgattc gatctgcgtc atgtcagcgc gtgccagcgt
    10021 caggaacttg gtggcgaatt cgcgcatttt ggtgcggtcg tctccgatca ggtcggccag
    10081 catcgacaga tcgatcagcg ctgaatctcc agaccatatc gtgctgtctt gcctgctcgg
    10141 tgcaggggcg tcgcgggtgg cttcttgcac cgctggtatc ggcagccagc gtgccagcgt
    10201 ggcgtagaag gcgctgggag tgaagggttt gcccatgaaa tcgttcatcc ccgcttgcag
    10261 gcaacgctgg cggttttcat ccgaggcatg cgcagtcacg gcgatgatcg gcagcttggc
    10321 ccaggcggga tcggagcgga tcgcctgcgt ggtttcaatc ccatccatca atggcatctg
    10381 cacatccatc agcacgcagt catagcggtt ctgctgcaac agttcgagtg cctcgcgtcc
    10441 gctccaggca gactgaaccg acgcgccggc actcttcagc aattcgccca tgaccatctg
    10501 actgagcgaa ttgtcatcgg cgaccaggat gcgtgccccg ttgaaattag cgaacagttc
    10561 gggcgatatc ttgcgctcct tgccggcggg aacggatgag ccggtcttgt cgaagcgagc
    10621 gctgaaccag aaggtgctgc ctttgccgag cgcgctctcc acgccgacct gaccgtcttc
    10681 catcatactg accagttcgc tgcagatggc taatcccagg ccggagcctt catgggtgcg
    10741 acttgatgag gcatcgactt gccagaacgg ggtgaacagg ggggggcgtt tgtccatatc
    10801 gataccgata ccggtatcct caacttcaaa gcgcagcttg caggaccgct cgtcttcctc
    10861 gaggatcctt gcacgaatga caattcgtcc ttgttccgtg aattttatgg cattgcacgc
    10921 atagttgagc agcacctgtt ctagccgagc cgcatcgcca tgaagtttca gggcatagag
    10981 ctcgggcggg atatcgaatg ccaactcgag tcccttgtgg ctcgctttgg gggtgagctg
    11041 gccgtcgagg tcgctcagta tttcgcgcag catgaaatcg aatggattca ggtgcagtct
    11101 gccggattcc aggctggaaa gatcgaggac gttatcgatg gtgtgtaaca gatgttcgcc
    11161 ggagaattgt atcttctcca gcagattgag cttgtggctg tcggtctcgt ttctgagtgc
    11221 cagatgcgtc atgcccagaa tcccgttcat cggcgtacgc aattcatggc tcatgttggc
    11281 gaggaattcg cttttcgcct gattcgcacg ctcggccagt cgcttggcgc gctcaagccg
    11341 cgcgctatcg agcaatgcgc gctcccggct gtacagcatc agccaggtga acaggctcag
    11401 tatcagactg acgactatgc cactgatggc gagggtgccg gtgcggctgt ggagcaattt
    11461 cgcatcgaag gcgggtttgg atgagaagga gagcgtccat agtctgtcgg cgatctggat
    11521 ttgccggacg ctcttgaatg ccgcagatgt gtcgcgcgcg ttttcactgc tgtaaagcag
    11581 cgtctctgcg gtcatctcag tgccgtcgta gatgtcgatg ttaatgtttc tggaggaagc
    11641 tccgatcagt tgcgctgcca gatcctgtat gcggaatgcc gccgacaccc agccgacgag
    11701 attggcgcga cgcgattctg cggtgttgtg tgcgacgccg ttccgataca ccggcatgaa
    11761 catcaggatg ctggtttgct gggcatctac tccctgtgcc agcgttaatt ttccggatac
    11821 gctgatctgt tcggaatcgc gagcgcgttc cagcgttgcg cggcgttcgg ccgtggcgta
    11881 attgtccagt ccgaactcgc gcaaattctc cccctgaaac ggctcgacat agatcgccgg
    11941 aacgtagaga tcgcggattc cttcgggatg gatggtgtaa tcggggaagc cctgcttgcg
    12001 tatggctgcg aggtgttgtt ccttttgcgc gccggatacg ctgatcgaga aagccacgct
    12061 ctggatgcct ggatgatgct cgtcaaggtg caggcctgcg acataggcgg agaattcgct
    12121 gcggcttacc tcgtctgagg cgctatacag ggcctggagt ccgtatagca cgtcctcgta
    12181 gtcggagagg cgttgcccga tattaaattc ggttcgatgt atcccatagt cgaactcgtt
    12241 ttgcagttcc gtctggagtt gccgctgcga gtcactccat atcagctgcg ttgccagcaa
    12301 gccgagaatg agcactaaca gggggagcag cagaatcggg cgcatgcggg gcaaggcgcg
    12361 cacggccttg ggaagaggat gctccgcttc gggattcgcg tcgtccggtg tcgggttcat
    12421 atcagggggt gccgctatag tcgatggctt accgcaaagt gaaccagctc ggcgatattt
    12481 ttcaggccga gcttgttgag caggttgctc ttgtgggtgc tgaccgtctt gtcgctgata
    12541 cagagctgct cggcgatctc gttgatggcg acgcccgtta ccagcaggcg gaatatctgc
    12601 tgttcccgtt gcgacagcat ggccagcgga tcagacgaat cagaatgcgg cccggtcgag
    12661 gcgaaagcca gctgctcggc gatttctggc ggcaggtatt tccccgttgc cgagaccttg
    12721 cgtatcgcat tcagcagcgc agggggggtg cagtttttgc tgatgaatcc agaagcgccc
    12781 gccttcatgg ctcgcaatac ggtttgcacc tcgttgtgca tgctgagcac cagaatgagc
    12841 agatccggat aggcactgcg cagccgcata atcagatccg catcgctcag gccgggcatg
    12901 ttcaggtcga gcaacaccag atcgaccggt gtgctacgca gcttctccag cagggttgtg
    12961 ccgtcgctcg cttcggcgat caccttgacg tctggcgaga gatccagata ttgggacaga
    13021 cctgcacgaa ccagttcgtg gtcatctgca atcatcaaac gaatcatctt gtttcctttg
    13081 gcggcatctt gagttttatg cgctgtgatt agaacggatt ttattcgtgg tgactatagg
    13141 cggatgccta aattaggctg gggtttttca tcgtctgggc ggcactgttg ccgtaagcgt
    13201 gcggcgcagc gcaatccgct ctgtaccgca ccttccagcg tggcgggata gtcgccagcg
    13261 gtgtagtcgc ctgccagcca caggcgggac agggcggtgt gctggacagg gcgttgcagg
    13321 tcgggggcgc agcagaaggt ggcacgcttc tcggcgataa ccttgaacca ggctggttgc
    13381 ctggaaatgc caaactcttc aagcagttcc ttgacgacct tccctgccag ggcttcctga
    13441 ctcaggtcct gatggatacc ttccgcgctg atgacggcgg cgatcaggcc gtgctggccg
    13501 ccgattttcc ccttgtcgaa cagccactgg ctgaagcgct ggtgcagtcc tagcatcggg
    13561 tgtggcaggg tgacgtcagc gggatattgc acatagacgg tgtagatcgg ctgatgctcc
    13621 agcgcgtcga tctgttgcac ggcgttggcg agtgcgggaa tcgggcgcag cagttttgcc
    13681 gcgacgaccg gcggtgcggc acagatgaca tggctgaagt gttgcgggcc tgcctccgtg
    13741 acaagttcga agccgtcgtg ttgggggccg atgctctcta ctccgcagga gagctgcacc
    13801 ttgccgccgc gctgctcgac gtaagttgcg gcccgttgcg ggaacagtgc ggtgaaatcc
    13861 agacgcggca gcagcatgtc ggaatcggcg cgcgattgat tcagcgcatc gcgcagtacg
    13921 ttcagcaaga cctgggcgga agccttgcgg atgggcgtgt tcagcgcggc aatgcacagc
    13981 ggctcccaca gcttgagcgt cagatcggca tcctgcccgt aacgggcgag cagctcggcg
    14041 accgtcatgt cgctggataa atggaaatcc atgcggcgca gcgcgagcat gaagcgagcg
    14101 gccttgatgc gctcgcgcca gctcagcccc tgagcgccga gcagcgcgat gagcagatgc
    14161 agcggagcgg gcaggtgggg cgcgcgcaat gagaactgcc cgtgcaggtc gagttgcagc
    14221 ggcagatgca ggaagtcttg ttcgaggtcg ccgccgacct gttcgatcag gcgcagggta
    14281 tcgcggtagc agccaagcag cagatgctgg ccgttgtcca gttgtgtgcc gttgtgcatc
    14341 accccgcgcg cgcgtccgcc gagctgtttc gcgctctcga acaccgtgac cggaatgcct
    14401 tgctcggcca gcgcgacggc tgccgccatg cccgcatagc cgccgccgat gatggcagga
    14461 ttgatcaatt atttctcccg tatgggagaa atgacgattg tcccctcgcc cgcttgcggg
    14521 agagggttaa ggagagggct gtcgtagctt ttagttctgg aaccatgcct tgaacgcgag
    14581 ccacagcttg taggtcggag tcagcgagac gcgtcccttc agtacatggc agcccgtggc
    14641 gacgacctcg tccagcgtcg cgctgtagat tgccgccatg atcagaccag tgcgctggct
    14701 cttgcgatcc acggcgggca ggtggtcgag tgcctgccgg tagtagcgtt gcgcgcgttc
    14761 gatctggaat gccatcagcc tctggaagtt ctcggtctcg ttcgcattca ggatgtccgc
    14821 tgcggtcacg ccaaactgct gcaactcgtc catcggcagg tagatgcggt tgcgtcgcgc
    14881 atcttcgccg acgtcgcgga tgatgttggt gagctggaag gcgatgccga ggtcgtgcgc
    14941 gtatttcagc gtctcgcgat cggtgtagcc gaagatctcc accgtcagca ggccgaccac
    15001 cgaggcgacg cggtagcagt acagttgcag ggatttgaag tcggcatagc gcgcctgctc
    15061 gaggtccatc tccatgccgt cgatgatctc aagcagatgt tcctgcgcca gattgaactg
    15121 cttcacgatg ggaaccagcg cctgggcgac cggatgctgc ggtgtgccgc cgtagatggc
    15181 gacgacttcg ttgcgccacc agttcagcgt ggtgcgcgcc acgttctcgt ccgagcactc
    15241 gtccaccaca tcgtcgactt cgcggcagaa ggcatacagc gcggtgatcg cgcggcgctt
    15301 gtcctgcggc aggaaacgga agctgttgta gaagctggag ccgctggcag cggccttgtc
    15361 ctggcaatat tggtctggag tcattgtgcg gagagtcaga gcggtgcgct tttggccagc
    15421 atgataaccc agtcgaacgg gcgcaagacc gggcgcttgt tgaacatgtc gtagtcggcg
    15481 gcttccagct tgtcgaggat gcgcaggccg cctgcgatga tcatgcgcat ctccaggccg
    15541 atgcgccccg tgaggatggt gcccagcgga cggcctgcca gcatcatctc gcgggcgcgc
    15601 tgtacctcga actgcatcag cgcgtgccag ctggcgtcag tcatgccctg tgcgatctgc
    15661 gcctcgctga cgccgaatcg cgccagatcg tcctgcggca ggtagacgcg accgatggcc
    15721 cagtccttcg ccacatcctg ccagaagttg atcagctgca gcgcgctgca gatcgcatcg
    15781 gagtaggcga gattgacggg cgtcgcctcc tcgtacagat gcaacaagag gttgccgacc
    15841 ggattggcgg agcggcggca gtagtccagc agctcgtcga aatcggcata gcgcttcttg
    15901 acgacatcct gcgagaacgc atcgagcagg tcatacagag gctgcatcgg cagcgcgtac
    15961 tgtttgacct cggcggccag attccggaac agcggcgtga gcggttcttc gtcggtctcg
    16021 atgcgtttca gttcggtgcg gaactcgtcg agccggcgcc gacgctcttc atccggcagc
    16081 tcgccttcgt cggcgatgtc gtccgccgcg cgcgcgaaat gatagatcgc cgccaccggc
    16141 ctgcgcagcc gcttgggcat cagcacggat gcgaccggga agttctcgta gtgctcgacc
    16201 gacgacatca gagcaaatcg ttcctgtgca gcatgaacac atgttcgtcg cctgcgctga
    16261 tttccagcca ggtgaacggc aggttggggt aggcggcttc cagtgcgtcg cggttgtggc
    16321 cgatctcgac gatcaggatg ccgttgtcgg tcaggtgttc ggcggcctgc gcgaggatga
    16381 cgcgagtggc gtccagcccg tcttcgccgc tgcccagaga gagcttgggt tcgtgcaggt
    16441 attccttggg cagcgcggcg accgattcgg cattcacata cggtggattg ctgatgatca
    16501 ggtcgtactt ccggccgccc aggttggtga acaggtcaga ttcgatcagc tcgatctgct
    16561 cattcaatcc ataatcgctg acgttgcgtg ctgctacggc cagcgcatca ggcgacaggt
    16621 cgacggcatc cacgctggca ttggggaagg catgggccgc gaggatggcg agacagccgc
    16681 tgcctgtgca catgtccagc acgctgccga tctcttccgg ttcggcgatc caggggctga
//
LOCUS       DASUIV010000238        10217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1360640, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000238 DASUIV010000000
VERSION     DASUIV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360640"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFW59_08635"
     CDS             complement(<1..307)
                     /locus_tag="VFW59_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364325.1"
                     /translation="MNTLSTSRPFDPGTCRHNQPSGPTENLQECDSMANEYRNSGMEG
                     RRMNLLHTTLKRIAKLGLLLTMGASMSACAGSMRWKEEVKLHDGQVVVEARYVNLGGY
                     "
     gene            complement(304..849)
                     /locus_tag="VFW59_08640"
     CDS             complement(304..849)
                     /locus_tag="VFW59_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364326.1"
                     /translation="MNAIGSKVDGSLWKEEVQLHDGSRIIVRRAQRYGELGEFDLSLH
                     VQEHTISFELPRTGKTLTFKSQYGADIERTNFNLLALHILDDTPFLIAEANLYRPYNK
                     WRKPALDYVVFKHDGKDWLHIPLMALPAGIKTVNLIVNGCAEDIERAARQHGYVPAEE
                     VRKLNRRLAKPKYHVILRETE"
     gene            complement(1121..1756)
                     /gene="cheD"
                     /locus_tag="VFW59_08645"
     CDS             complement(1121..1756)
                     /gene="cheD"
                     /locus_tag="VFW59_08645"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HEX5364327.1"
                     /translation="MAIAHIPRPPLALPGFEHVRRNWNNTYESYAARLLPGEYYVTIN
                     DEGIYTTLGSCISACIRDRVSGVGGMNHFMLPASADADGWKSTSLSSATRYGNFAMEH
                     LINDILKNGGKRQNLEVKLFGGGRILANMTDVGNRNIVFVRDYLATEGLKVTAEDVGE
                     IYPRMVVYFPATGKVRVKRLRSLHNNVIVEEETRYLSAIESKPVGGDIELF"
     gene            complement(1775..2659)
                     /locus_tag="VFW59_08650"
     CDS             complement(1775..2659)
                     /locus_tag="VFW59_08650"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5364328.1"
                     /translation="MADNDDIVKLVRSVDGIATLPHIFIRINQLVNDPDSSMHDIARV
                     VSQDLSFSMRLLRVANSPFYGFSSSIDTVDKAVSVIGTSKIRSLALSMSVASSFSSLL
                     NDLVSMDNFWRHSLYCAVIARILAKQTGKCDPEVMFTAGLLHDIGELIIFNRLPEQAR
                     EVLLLVQDSVDDLPMHVAERQILGFDHGQVGLELARQWNLPPMLEECIGYHHAVAEVQ
                     HFPREVALVHLANILAQMAELDTLDPADVTAIDPHAWELTGLGVEIVELVVREAQEEI
                     VETEKLFIDQSSVNAATK"
     gene            complement(2745..3488)
                     /gene="fabG"
                     /locus_tag="VFW59_08655"
     CDS             complement(2745..3488)
                     /gene="fabG"
                     /locus_tag="VFW59_08655"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX5364329.1"
                     /translation="MTLQGQIALVTGASRGIGQAIALELGKQGATVIGTATSENGAQA
                     IGAYLDAAGIKGAGLALNVNDAAQTEQVLGAIRQQFGEISILVNNAGITRDNLLARMT
                     DEEWDDVLTTNLKSVFRLSRAVLRAMMKARGGRIISISSVVGSMGNPGQANYAASKAG
                     MVGFTKSLAQEIGSRNITVNCVAPGFIDTDMTQGLGEEQRAKLVEHVPLKRLGQPADI
                     AAAVSFLAGPGAAYITGVTLHVNGGMYME"
     gene            complement(3602..4534)
                     /gene="fabD"
                     /locus_tag="VFW59_08660"
     CDS             complement(3602..4534)
                     /gene="fabD"
                     /locus_tag="VFW59_08660"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5364330.1"
                     /translation="MTKFAFVFPGQGSQSRGMMNGYADFAAVRDTFAEASDVLKQDLW
                     QLVAEGEDAELNATVNTQPIMLTAGVAVYRAWQSQNGATPAMMAGHSLGEYTALVASG
                     ALSFADALPLVRYRAQCMQTAVPEGVGGIAAILGLDDEAVRAVCIEGAQGEVLEAVNY
                     NSPGQVVIAGNRAAVERGMELAKARGAKRAIMLPMSVPSHCSLLKGAAEQLRTYLNDV
                     AVQAPRIPVLHNADVAAYNDAASIKDALVRQLYSPVRWVETVLEFGKQGITHNVECAP
                     GKVLAGLNKRIDTNQQALAINDGEALKAVLVTLA"
     gene            complement(4543..5514)
                     /locus_tag="VFW59_08665"
     CDS             complement(4543..5514)
                     /locus_tag="VFW59_08665"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX5364331.1"
                     /translation="MSSPKHSRIIGTGSYLPAKTLTNYDLEKIVDTSHDWIFSRSGIV
                     ERHVAADGEFTSDLALQASRKAIEAAGIEAGEIDLIIVATTSPDHIFPSTACILQDKL
                     GIKNGCAAFDMQAVCGGFVYALNTADMYIRGGQAKTVLVVGAEVLSRMLDWNDRTTCV
                     LFGDGAGAVVLRASETPGIVACKLHSDGSHRGMLKAEGNIRNGEVDGDPFIKMDGQAV
                     FKFAVKVLSDVVEEVLEDEKLAGSDIDWLVPHQANIRIIEATAKKLGLGMDNVVVTVA
                     KHGNTSAASIPLALDTAVRDGRIKPGQNIMLEAVGGGFTWGAVLIKW"
     gene            complement(5514..6551)
                     /gene="plsX"
                     /locus_tag="VFW59_08670"
     CDS             complement(5514..6551)
                     /gene="plsX"
                     /locus_tag="VFW59_08670"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX5364332.1"
                     /translation="MDVTLAIDAMGGDHGTTVTIPAAVEYLKQFPGDTIVLVGIPDAI
                     ESELRALGVQAHAHLRIHPATEVVGMDEQPQSALRGKKDSSMRVSINLVKSGEASACI
                     SAGNTGALMATARYVLKTLPGIDRPAIASYLPTKNGQVCMLDLGANTDCTAEHLLQFA
                     LMGSTLVTALEHKPNPTIGLLNIGSEDIKGNEVVKQAAELLRASDLNFYGNVEGDDIF
                     KGITDVVVCDGFVGNVALKTAEGVAKMMGGFLREGFGQNLYTKFSALIALPVLKAFKH
                     RLDHRRYNGASFLGLKGIVVKSHGSADVFAFRCAIERAAEEAREGMLHHISEHIEKWH
                     HERQNTEQGVV"
     gene            complement(6632..6811)
                     /gene="rpmF"
                     /locus_tag="VFW59_08675"
     CDS             complement(6632..6811)
                     /gene="rpmF"
                     /locus_tag="VFW59_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5364333.1"
                     /translation="MAVQQNKKSPSKRGMHRAHDFLTNAPTAIEPTTGEVHLRHHISP
                     TGFYRGKKVVKTKGE"
     gene            complement(6833..7291)
                     /locus_tag="VFW59_08680"
     CDS             complement(6833..7291)
                     /locus_tag="VFW59_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX5364334.1"
                     /translation="MSGIVSVAEMPRLLDTLDNPAGSISYSVRGGEGRQGVPFLDVTM
                     TGSCELRCQRCLNAMAHAIQLDTRLLLRDQAGLDALDEGVAGGEEEEFDSILADERLD
                     VLSLLEEEILLSLPIAPRHEPGVCQAADGGSGQKEATHPFAALANLKVVK"
     gene            7402..7986
                     /locus_tag="VFW59_08685"
     CDS             7402..7986
                     /locus_tag="VFW59_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX5364335.1"
                     /translation="MSSQLLVLASTSIYRSELLKRLQLPFETAAPNVDETPRPDESAR
                     ATSVRLAQEKARAVAPAYPDALIIGSDQVALLDGQQLGKPLTHDNAVKQLRAMRGKTT
                     TFYTALTLLNSKTGNLQTEVAKNHVTLRDLDDAEIESYLQKEQPYQCAGSAKSEGLGI
                     ALMSSMSGDDPNALIGLPLILLTEMLRRENVRPF"
     gene            8070..8786
                     /locus_tag="VFW59_08690"
     CDS             8070..8786
                     /locus_tag="VFW59_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5364336.1"
                     /translation="MITGTLYLIPCTLGDTPAEQVLPQHVIDIARKLKHFVVEQPKTT
                     RQFLSALKHEQPIQSLHFATLNEHTESRELADLLTPLLAGEDVGIVSEAGCPGIADPG
                     ADLVNLAHRKGIRVVPLVGPSSILLALMGSGLNGQCFAFHGYLPIAEADRNKTISALE
                     AESAKRKQTQLFIETPYRNEKLFSALLAQCRPQTLLCVACDITLPGELIQTRTIAQWK
                     SQPAPQLNKRPSLFLLLANG"
     gene            8869..9291
                     /gene="dksA"
                     /locus_tag="VFW59_08695"
     CDS             8869..9291
                     /gene="dksA"
                     /locus_tag="VFW59_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX5364337.1"
                     /translation="MPVQPQKPIVPYKAKKGEEYMNPKQLDHFRQILNGIKAGLGEDI
                     DRTVHTMQDEATVFADPNDRATQESDMSLELRNRDRERKLIKRINEMLAKIDAEDYGY
                     CDNCGIEIGLNRLEARPTATLCIDCKTLDEIREKQMAK"
     gene            complement(9453..>10217)
                     /locus_tag="VFW59_08700"
     CDS             complement(9453..>10217)
                     /locus_tag="VFW59_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX5364338.1"
                     /translation="KNGGITVAAKLKMAWSPEQIAGWLKCTYPDDEDYQVSHETIYRS
                     LFIQARGVLKKELLQHLRRTRAMRRSRHHTQKTGDHGRIIDAVSISERPATAEDRAVP
                     GHWEGDLLCGTLSSQIATLVERQTRYVMLVKVAGKDTETVINALIKHAHKLPKELYKS
                     LTWDRGKEMAGHKRFTLATDISVYFCDPQNPWQRGTNENTNGLLRQYFPKGMDISGYT
                     QAQLNGVARKLNERPRKTLNYETPAERFNQLVASTG"
BASE COUNT         2110 a         3176 c         2782 g         2149 t
ORIGIN      
        1 gatagccccc caagttcaca taacgcgcct ccacgacaac ttgcccatcg tgcagcttca
       61 cttcttcctt ccagcgcatc gatcctgcac aggcactcat gctcgccccc atcgtcaaca
      121 gcaatccaag tttcgcaatg cgtttcagtg tcgtatgtaa taggttcatg cggcggccct
      181 ccatacctga attgcgatat tcgttcgcca tgctgtcgca ctcctgtaaa ttttctgttg
      241 gtccgctcgg ctgattgtgt cggcatgtgc cgggatcgaa cgggcgggat gtagataagg
      301 tattcattcg gtctcccgca ggatgacgtg gtatttgggt ttggccagcc tgcggttcag
      361 cttgcgcact tcttctgccg gcacgtagcc atgctggcgg gcggctcttt cgatgtcttc
      421 ggcgcagccg ttgacgatca ggttgacggt ttttattccg gcaggtaacg ccatgagcgg
      481 gatatgcagc cagtctttgc cgtcgtgttt gaaaacgaca tagtccaaag ccggcttccg
      541 ccatttgttg tagggccggt acaggttggc ttcggcgatc aggaacggcg tgtcatccag
      601 gatgtgcagg gccagcaggt tgaagttggt gcgttcgatg tctgcgccgt actggctctt
      661 gaaggtcagc gtcttgccgg tgcgcggcaa ttcgaaactg atggtgtgct cctgaacatg
      721 cagggacaga tcgaattcgc ccagttcgcc ataacgctgc gcacgcctga cgatgatccg
      781 gctgccgtca tgcaactgca cttcttcttt ccacaggctg ccgtccactt tggatccaat
      841 ggcattcatg ctcatatctg cctccatcat ctaaatcaac tccggcacct aaaccgaatg
      901 agttgtactt cgaacggaat gggaatacgc gcgagacggc ttgttgggca caataaatcc
      961 cactggatat atgtgggcat tattccaaat cgacgactaa cagcctagct accgaaatgg
     1021 atagctaggc aagcgacagg atttgtgatt ggggctgccg aaaacggcaa gatacgatct
     1081 gttttcgctt caagcttgga ctgcttctgc agcctatgcc tcagaacagt tcgatgtcgc
     1141 cgccgaccgg tttgctctcg atggcgctga ggtaacgggt ttcttcttcc acgatgacat
     1201 tgttgtgcag ggaacgcagg cgcttgaccc tgaccttgcc ggtggccggg aagtagacca
     1261 ccatgcgcgg gtagatttcg cccacatctt ccgccgtcac cttcaggccc tcggttgcca
     1321 gatagtcgcg cacgaagacg atgttgcgat tgccgacatc ggtcatgttc gccaggatgc
     1381 gccccccgcc gaacagcttg acctccagat tctgacgctt gccgccattt ttcaggatgt
     1441 cgttgatcag gtgctccatc gcgaagtttc cgtaacgggt ggccgaactc aggctggtcg
     1501 atttccagcc gtcggcatcg gcagaagcgg gcagcatgaa atgattcatg ccgcctacgc
     1561 cggagacccg gtcacggatg caggcggaga tgcacgaacc cagcgtggta tagatgccct
     1621 cgtcattgat ggtgacgtaa tactcccccg gtaacaggcg agccgcgtag ctttcatagg
     1681 tgttgttcca gttgcgccgg acatgctcga aaccgggcaa tgccaagggc ggacggggaa
     1741 tgtgggcgat agccataatg attgtctacc gggattattt agttgcggca ttgacagagg
     1801 attggtcgat gaacagcttc tctgtctcga cgatctcctc ctgcgcctcg cgcaccacca
     1861 attcgactat ttcaacgccc aagccagtca actcccatgc atgcggatct atcgccgtga
     1921 catcggcggg atccagggta tcgagctcgg ccatttgcgc cagaatgttg gccagatgca
     1981 ccagtgcgac ttcgcgcgga aaatgctgaa cttcagcgac ggcatgatgg tagccgatgc
     2041 actcctccag catcggcggc aggttccact ggcgggccag ctccagaccg acctgcccgt
     2101 ggtcgaaacc cagtatctgg cgttcagcca cgtgcatggg cagatcatcg acgctatcct
     2161 gcacgagcag caagacttct ctggcctgtt caggcagacg attgaaaatg atcagctcgc
     2221 cgatatcgtg cagcaagccc gccgtaaaca tgacttcagg atcgcacttg cccgtctgtt
     2281 tcgccaggat gcgggcgatc acggcgcaat acaggctatg ccgccagaaa ttgtccatgg
     2341 acaccaagtc gttgagcaag ctggaaaagc tgctggcaac cgacatggac agcgccagac
     2401 tgcgtatctt gctggtgccg atcaccgaca ccgctttatc gacggtatcg atggaggaag
     2461 aaaatccgta gaacgggcta ttggcaaccc gcaacaggcg catggagaac gagagatcct
     2521 gactcaccac tcgggcgatg tcgtgcatgc tgctgtcggg gtcgttgacc agctgattga
     2581 tgcggatgaa gatgtgcggc agcgtggcga tgccatccac gctccgtacc aatttaacga
     2641 tgtcgtcatt gtctgccatg acccgaacct tctcacgatg ccgtcagaca gtcaagcaac
     2701 gcccccatcc ttgacgaggg ccgacgtggc ttcttgcgaa acccctattc catatacatc
     2761 ccgccgttca cgtgcaaggt gacgccggtg atgtaagcgg cgccggggcc ggccaggaaa
     2821 gagacggctg ccgcgatatc ggcaggctgg ccgaggcgct tcaacggcac atgctccacc
     2881 agcttggcgc gctgttcttc gcccaagcct tgggtcatat cggtgtcgat gaaaccgggc
     2941 gcgacgcagt tcacggtgat gttgcggctg ccgatctcct gcgcgagcga tttggtgaaa
     3001 ccgaccatgc ccgccttgga ggcggcgtaa ttggcctgcc ccgggttacc catgctgccg
     3061 accaccgacg agatgctgat gatgcgaccg ccacgggcct tcatcatcgc gcgcagcact
     3121 gcgcgggaaa gacggaacac cgatttgagg ttagtggtca gcacgtcatc ccattcttcg
     3181 tcggtcatgc gcgccagcag attgtcgcgg gtgataccgg cgttgttcac cagtatcgag
     3241 atctcgccga actgctggcg gatagcgccc agcacctgtt cggtctgcgc cgcgtcgtta
     3301 acgttcaaag ccagaccggc acccttgatt ccggccgcat ccagataagc gccgatggcc
     3361 tgagcaccgt tttcgctggt cgcggtgccg atcacggtcg cgccctgctt gcccagttcc
     3421 agtgcgatgg cctgtccgat gccgcgcgat gcgccggtca ccagtgcgat ttgtccctgc
     3481 agtgtcattt gttatctccc caaagttatc aacttccccc gttgcaaaag ggggatggag
     3541 ggggatttga actccgatac gaccaatccc cctgccccct ttttcaaagg gggaaaaatt
     3601 ctcaggccaa cgtaaccagc acagccttca gcgcttcgcc gtcgttgatg gccaaggcct
     3661 gctgattggt gtcgatacgc ttgttcaaac cggccagcac cttgcccggc gcgcattcga
     3721 cgttgtgggt gatgccctgc ttgccgaatt ccagcacggt ctcaacccag cgcaccggcg
     3781 agtacagctg gcgcaccagc gcgtccttga tcgaagcggc gtcgttgtag gcggcgacat
     3841 cggcgttgtg cagcaccgga attcttgggg cctgcaccgc gacatcgttc agataagtgc
     3901 gcaactgttc cgcagcgccc ttcagcaggc tgcaatgcga cggcacgctc atcggcagca
     3961 tgatcgcgcg cttcgcacca cgggccttgg ccagttccat gccgcgctcg actgcggcgc
     4021 gattgcccgc gatcaccacc tgaccgggcg aattgtaatt caccgcttcc agtacctcgc
     4081 cctgcgcgcc ctcgatgcac accgcgcgca ccgcctcgtc gtccagaccg aggatcgccg
     4141 cgatgccgcc cacgccttcc ggtacggctg tttgcatgca ctgcgcgcgg tagcgcacca
     4201 gcggcagcgc atcggcgaaa ctcagggccc cgctagcgac cagcgcggtg tattcgccca
     4261 gactgtgtcc tgccatcatc gcgggcgtcg cgccgttctg cgactgccag gcgcggtaca
     4321 ccgccacacc ggcggtcagc atgatcggct gggtgttgac cgtggcgttc agctcggcgt
     4381 cttcaccttc cgcgaccagt tgccacaggt cttgtttcag cacatcggag gcttcggcga
     4441 aggtgtcgcg caccgcagcg aaatcggcgt aaccgttcat catgccgcgc gattgggagc
     4501 cttgtccggg aaatacgaat gcgaatttag tcatcagttt acctaccact tgattagaac
     4561 ggcgccccag gtgaacccgc cacccacggc ttccagcata atgttttgtc ccggcttgat
     4621 gcgtccgtcg cgcacggcgg tgtccagcgc cagcggaatc gacgccgccg aagtgttgcc
     4681 gtgcttggcg acggtgacca ccacattgtc catgcccaga cccagtttct tggccgtggc
     4741 ttcgatgatg cggatgttgg cctgatgcgg caccagccag tcgatatcgc tgccggccag
     4801 cttttcatct tcgagcactt cctcaactac atcgctcaac accttgaccg caaacttgaa
     4861 aacagcctga ccatccatct tgatgaacgg atcgccatcg acctcgccgt tacggatatt
     4921 gccctcggcc ttcagcatgc cgcgatggct gccgtcggaa tgcagcttgc aggcaacgat
     4981 gcccggcgtc tcggaggcgc gcaacaccac cgcaccggct ccgtcgccaa acagcacgca
     5041 ggtggtacgg tcgttccagt ccagcatgcg cgacagcact tccgcgccga ccaccaaaac
     5101 ggtcttggcc tgccctcctc ggatatacat gtcggccgta ttcagcgcat agacgaagcc
     5161 gccgcagacc gcctgcatgt cgaatgccgc acagccgttc ttgatgccca gcttgtcctg
     5221 cagaatgcag gcggtgctgg ggaagatatg gtccggcgag gtggtcgcga cgatgatcag
     5281 atcgatctcg cccgcctcga taccggcggc ttcaatcgcc ttgcggctgg cctgcaaggc
     5341 gagatcgctg gtgaattcgc cgtcggccgc gacatgacgc tcgacgatgc cgctgcgcga
     5401 aaagatccag tcgtgcgacg tgtcgactat cttctcgaga tcgtaattgg tcagcgtctt
     5461 ggcgggcaga taactgccgg taccgatgat ccttgaatgc tttggggaag acatcagacg
     5521 actccttgct cagtgttttg acgctcgtga tgccactttt cgatatgctc gctgatgtgg
     5581 tgcagcattc cctcgcgcgc ctcttcggcc gcacgttcga tcgcgcagcg gaaggcgaag
     5641 acatctgccg aaccgtgact cttcaccaca atccccttca acccgaggaa actagcaccg
     5701 ttgtagcggc gatgatccag ccggtgcttg aacgccttga gtacgggcag cgcgatcaat
     5761 gcagaaaatt tggtgtacag gttctgaccg aagccttcgc gcaggaagcc ccccatcatt
     5821 tttgccacgc cctcggcggt cttcaacgcg acattgccga cgaaaccgtc gcacaccacc
     5881 acgtcggtga tgcccttgaa gatgtcgtcg ccttcgacgt tgccgtagaa attcaggtcg
     5941 ctggcacgca acaactcggc agcctgtttg accacctcgt tgcccttgat gtcttcgctg
     6001 ccgatattca gcaggccgat cgtgggatta ggcttgtgct ccagcgccgt caccagcgtg
     6061 gaacccatca gcgcaaactg caacaggtgc tcggcggtgc aatcggtatt ggcgcccaga
     6121 tcgagcatac agacctggcc gttcttggtc ggaagatagg aagcgatggc aggccggtcg
     6181 atgcccggca gggtcttcag cacgtagcgt gccgtggcca tcaaggcgcc cgtattgcct
     6241 gcgctgatgc aggcggaagc ctcgccgctc ttgaccagat tgatactgac acgcatcgag
     6301 gaatccttct tgccgcgcag cgcggactgc ggctgctcgt ccatgcccac cacttcggtg
     6361 gcgggatgga tgcgcaaatg cgcatgcgcc tgcacgccca aggcgcgcag ttcggattcg
     6421 atggcgtcgg gaatacccac caaaacaatg gtgtcgccgg gaaactgctt aagatattcg
     6481 actgcggcgg gaatggtgac cgtcgtgccg tggtcgcctc ccatcgcatc tatggctaat
     6541 gtgacgtcca ctaagcgatc cctgggaaga taaagaaagc agaaaagaaa acgcagccgg
     6601 tcatcacgac cggctgcgtc actaccaaga cttattcgcc cttggttttg actactttct
     6661 tgccacggta gaaaccggtc gggctgatgt ggtgacgcag atgcacttca ccggtagtcg
     6721 gctcgatcgc ggtcggtgcg ttggtcagga aatcgtgagc acggtgcata ccacgcttgg
     6781 aaggggactt tttattctgc tgaactgcca tgttaactac tcctcaaaaa aactatttaa
     6841 ccactttcaa attcgctagc gccgcaaaag gatgggttgc ctccttttgg ccgctgccac
     6901 catctgccgc ctgacacaca cccggctcat gcctgggcgc aattggcaga ctcaacaaaa
     6961 tctcttcttc cagcagactc agcacatcca gccgctcatc tgcaagaata ctgtcgaact
     7021 cttcttcctc gccgcctgcg acgccttcat ccagcgcatc cagaccggcc tgatcgcgca
     7081 gcaacaagcg cgtatccagc tgtatcgcat gagccatggc gttcaggcaa cgctgacaac
     7141 gcaactcaca gcttccggtc atcgtgacat ccagaaacgg aacgccttgc ctgccctctc
     7201 ccccgcgcac cgaatagctt atgctgcccg ccggattatc cagcgtatcg agcaacctgg
     7261 gcatctcggc gaccgacact atcccgctca tttgcttgcc atttctggca aaatccaagc
     7321 tatcgattaa aggtcgtgcg tacatgaggc gcgcatgata tatttttcgc tgccctctgt
     7381 caaaaaacct aaggaatatt cttgagttct cagctgcttg tcctcgcatc aacctcgatt
     7441 tatcgcagtg aactgctaaa acgactacaa ctccccttcg agaccgccgc accgaatgtg
     7501 gatgaaaccc ccagacccga cgaatccgcc cgagccacat cggtgcgcct cgcacaggaa
     7561 aaagcccgcg ctgtcgcccc cgcttacccc gacgcgctga tcataggctc ggatcaggtc
     7621 gccctgctgg acggccagca actcggcaag ccgctcaccc acgacaatgc ggtgaagcag
     7681 ctgcgcgcca tgcgcggcaa gacgacgacc ttttataccg cgctgacgct gctaaacagc
     7741 aagaccggca atctgcagac cgaagtcgcg aagaatcacg tcaccctgcg cgatctcgac
     7801 gatgcggaga tcgaatccta tctgcagaag gaacagccct accaatgcgc cggcagcgcc
     7861 aaatccgaag gtctgggcat cgcactgatg agcagcatga gcggcgacga cccgaatgcc
     7921 ctgatcggcc tgccgctgat cctgctgact gaaatgctgc gtcgcgaaaa cgtgaggcca
     7981 ttctaagcca tgacctcacc cctcccccca accctctccc gcaagcggga gagggagcaa
     8041 acgattcgct acgcgaggcc ccgtaaacca tgataactgg aacgctttac ctcatcccct
     8101 gcacgctggg cgacacgccc gccgagcagg tgctgccgca acacgtcatc gacatcgccc
     8161 gaaaactgaa gcacttcgtg gtggaacagc ccaagaccac gcgacagttc ctctccgcgc
     8221 tcaagcacga acagcctatc cagtcgctgc acttcgccac cctcaacgag cacaccgaat
     8281 cccgcgaact ggccgatctg ctcaccccac tgctggcggg cgaggatgtc ggcatcgtct
     8341 ccgaggctgg ctgccccggc attgccgacc ccggcgcgga cctggtcaac cttgcgcatc
     8401 gcaagggcat ccgcgtcgtg ccgctggtgg gcccctcctc catcctgctg gcgctgatgg
     8461 gctccggcct gaacgggcag tgcttcgcct ttcacggcta cctgcccatc gccgaggcgg
     8521 atcgcaacaa gaccatctcg gcactggaag ccgagtcggc caaacgcaaa cagacccagc
     8581 tgttcatcga gacgccttat cgcaacgaaa aactgttttc cgcgctgctc gcccagtgcc
     8641 gtccccagac cctgctatgc gttgcctgcg acatcaccct gcccggcgaa ctgatacaga
     8701 cacggaccat cgcgcaatgg aagtcgcaac ccgcccccca gctgaacaaa cgtcccagct
     8761 tgttcctgct gcttgccaat ggctagcaca gacatcaatc tggttgctca aacccccgct
     8821 ttctggtatt aaagcggact ttagatttta tcccgttgga gaagtgcaat gccggtacag
     8881 ccacaaaagc ccatcgtccc ctataaagca aagaagggcg aggagtacat gaaccccaag
     8941 caactcgacc atttccgcca gatattgaac ggaatcaagg ccgggcttgg cgaagatatt
     9001 gaccgcacgg tgcataccat gcaggacgag gcgacggtat ttgccgaccc caatgaccgc
     9061 gccacgcagg aatccgacat gagtctggaa ctgcgcaacc gtgaccgcga acgcaagctg
     9121 atcaagcgca tcaacgagat gctggccaag atcgacgccg aagactacgg atactgcgac
     9181 aactgcggca tcgaaatcgg cctgaaccgc ctcgaagccc gccccaccgc gacgctgtgc
     9241 atcgactgca agaccctgga cgagatccgc gaaaagcaga tggccaaata acatttggcc
     9301 atctgctttc ggtgaagact tatatgcgcg tagcgcatgt tagtcggaag caaaaacaaa
     9361 tggcgaagac agttacaagc agaaaagacc acggttttcc gtggcctttt gctttcggag
     9421 gaggcggtga aggtctgctt ctcggtgcgg attcaacccg tcgatgcaac aagttggtta
     9481 aatcgttcag ccggtgtttc atagtttagc gtcttccttg ggcgttcatt tagcttcctc
     9541 gccaccccat ttagctgtgc ttgcgtgtat ccggagatgt ccataccttt tgggaagtac
     9601 tgcctcaaca ggccattggt attttcattc gtcccgcgct gccaaggatt ctgagggtcg
     9661 cagaaataga cactgatgtc ggtcgccaag gtaaaacgct tgtggcctgc catctcctta
     9721 ccccgatccc acgtcagcga tttgtagagc tccttcggca gcttgtgagc atgtttgatc
     9781 aatgcattta tgacggtctc ggtatctttc cccgctactt tcaccaacat cacatagcgt
     9841 gtctggcgct caacaagtgt cgcaatctgg ctacttaaag tcccacacaa caggtcgccc
     9901 tcccagtgac ctggcacggc ccggtcttct gccgtggccg gacgctcgct aatcgatacc
     9961 gcgtcaataa ttctgccgtg atcgcctgtc ttctgagtgt gatggcgcga acggcgcatt
    10021 gctcgcgtgc gtctcaaatg ttgcaacaac tctttcttca agacaccacg ggcttgaata
    10081 aacaggctgc gatagatggt ctcgtgcgac acctgatagt cctcgtcatc tggataggta
    10141 cattttagcc acccggcaat ctgctccggt gaccatgcca ttttcagttt ggctgccacg
    10201 gtaatccccc cattttt
//
LOCUS       DASUIV010000239         8007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1177100, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000239 DASUIV010000000
VERSION     DASUIV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177100"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFW59_08705"
     CDS             <1..599
                     /locus_tag="VFW59_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5364339.1"
                     /translation="MGASISQNSENARVTDGMAAQAAKQATDGGEAVKETVAAMKQIA
                     GKIGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLAERSQVAAQEIGDL
                     ASGSVAKAEQAGRLLEQMVPAISKTSDLVQEITAASEEQSSGAAQINTAMNQLNQITQ
                     QNASATEELAVTAQEMSGQAEQLQRLMGFFRVNGAARA"
     gene            945..2450
                     /locus_tag="VFW59_08710"
     CDS             945..2450
                     /locus_tag="VFW59_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5364340.1"
                     /translation="MPVTNVERHLKEGEYIVSKTNLKGQITYVNRPFLEISGYTEEEL
                     LGQPHNMIRHPDMPEGAFADLWKTLQSGKPWRGMVKNRCKNGDYYWVDANANPFWKDG
                     QIVGFMSLRTKPTRAQVEAAEALYRRFREGTARGLTVREGAAVRTGLLGGIARLGKMG
                     IKARASIASVLLVASLGWLGYGADASLRPVLVGAGVALTAYIWWLLAHKILHPLQEGV
                     RSCQMVSSGNLILEIEEEFSGEIGHLMHAIKTMAMNAESIVADVSYASASIGVSSSEV
                     ATTAQSMSSDINAQAASVEQTSASVEQISASINRNNDNARSTGSMASQVARQAIQGGE
                     VVKDTAAAMKQIAGKIGIIDDIAYQTNLLALNAAIEAARAGEYGKGFAVVAAEVRKLA
                     ERSQLAAREIGELAAISVDKSAQAGRLLDEIVPNINKTSELVQEMATASGEQATGAAQ
                     INAAMTQLNQITQQNASATEELAATAEAMSTQARSLEQMMGFFSVHDAVCR"
     gene            2812..3201
                     /gene="flhD"
                     /locus_tag="VFW59_08715"
     CDS             2812..3201
                     /gene="flhD"
                     /locus_tag="VFW59_08715"
                     /inference="COORDINATES: protein motif:HMM:NF017094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEX5364341.1"
                     /translation="MSFMQFHADIRDINLTYLMLAQQMIREDRAAAIFRLGISQNMVD
                     VIDKLTPPQVIKMASSNILLCRFRFDENLLLNMLTDYSKDRLMADAHTTIMMAGQGVE
                     DLVPERGDARKLVFSGSESRRQELLFA"
     gene            3643..4299
                     /locus_tag="VFW59_08720"
     CDS             3643..4299
                     /locus_tag="VFW59_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364342.1"
                     /translation="MSEQNESCARPRRVRLGTPELRGTGKTELALIVEVNEAIKQSIA
                     VSEGISLIAVNANLVAGRAGSRAAGFCIVAGELRRFSDNMAHTNQGWSKLIFELVRET
                     ALNRNQSRYFNLLKAAARGSDKAQAAIAEARERSLNELLITTAHNSARVIELQGLIQR
                     AEKQQVMGVMIARSALIESAYGGAMRPVLDQIATSIDASIGDLISNGRKVELMMSRVN
                     "
     gene            4299..5048
                     /locus_tag="VFW59_08725"
     CDS             4299..5048
                     /locus_tag="VFW59_08725"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5364343.1"
                     /translation="MKKTTVIYANGEHKWLAVSRDPERPNYLIDTNEYLITDGKEALL
                     TDPGGSEIFPAVFSALCEEFDPARITHLFASHQDPDIVSSLALWLQFNPQLRCYTSWL
                     WTTFIPHFGGNDQTFIPLPDEGLKFQLDRIELEAMPAHYLHSSGNFHLYDAKAKLLFT
                     GDIGAALLPETENDLFVKDFDKHIAYIKGFHQRWMGSKEAVLDWCERASNMEIDMLCP
                     QHGSIYQGQDVSRFINWLSELEVGSGVRRKG"
     gene            5052..5384
                     /locus_tag="VFW59_08730"
     CDS             5052..5384
                     /locus_tag="VFW59_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364344.1"
                     /translation="MYEQDIRLNWRAAIVLDNGRQVVHGRLQRLGRKKVVVCADHNLS
                     PGYRCNLAVMLPKSRADETGIVVEASATIAASVLSAMQFYITLDRVTFEGRAQALLDE
                     RLRILGKM"
     gene            5552..6820
                     /locus_tag="VFW59_08735"
     CDS             5552..6820
                     /locus_tag="VFW59_08735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5364345.1"
                     /translation="MNVLIVEPSRTVAFTLSALFGKYGFEPRVARNGQEALDMLKVRP
                     AELLCFAFELGDMDGIEFFVSAKSHKLVHHQPGLMFASTQRKTLINRALMAGVTECFS
                     KRNLDQLEQFVERFASSTHVRISGRVLLVEDSPSAAMFYHQILKRMGLHVDHCKSAEE
                     AIESFAVQAYDLVVTDYILAGAQTGFSVIRAVRESLGKSALTPILAISSFDDMARKVE
                     ILRNGANDFVGKPVVPEELEVRVFNMLKMQKLMQRLESQHAVMKDIAMRDPLTSLYNR
                     HYLNEITPGLIGEAHEGGQNLALMVVDVDHFKQINDTHGHKRGDEVLQQIAKSLQSVC
                     RSDDLVARFGGEEFVILLPGLGLAEATARGEAMRARIAGLEQPGGLALTASIGIAALI
                     PGENYDELFHRADGAMYRAKQGGRNRVAAA"
     gene            7012..>8007
                     /locus_tag="VFW59_08740"
     CDS             7012..>8007
                     /locus_tag="VFW59_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX5364346.1"
                     /translation="MFKHMKIGMRLWLLVLVAITALIFTAAQSLLNLRHSMLEDRQTK
                     TRHVVETAYGVMSYYGALSATQKISPEEARTQAINILRSMRYEEKEYFWVNTLEPRMV
                     MHPMKPELDGKDLSGLKDPNGKLIFVEFANTAKHGGGFVDYFWPKPGSDKPVAKISYV
                     KQYEPWGLVIGSGIYLDDVDSAFMREVLKLGGFAGGALLIIGFSAWVIARSITRPLNL
                     AMDAANKIAAGDLSANIQVDSKDETGQLLVAMKNMNDTLKGLMTDTDVLVKAAAVGDL
                     NTRADASKYQGDFRKLVQGVNDTVTNIAEPMKVTSDYINQIAKGVIPAQITTAYQG"
BASE COUNT         1805 a         2081 c         2541 g         1580 t
ORIGIN      
        1 agatgggcgc ttcgatcagc cagaactctg agaacgccag ggtcaccgac ggcatggcag
       61 cgcaagccgc caagcaggcg accgacggag gcgaggcggt gaaagaaacg gtcgccgcga
      121 tgaaacagat cgcgggcaag atcggcatca tcgacgacat cgcctaccag accaatctgc
      181 tggcactgaa cgcggcgatc gaagcggcgc gtgcgggcga acacggcaag ggtttcgcgg
      241 tggtcgcagc cgaggtgcgc aagctggcgg aacgttctca ggttgccgca caggagatcg
      301 gcgatctggc cagcggttcc gtggccaagg cggagcaggc tggcaggctc ttggagcaaa
      361 tggttccggc gatcagcaag acctccgatc tggtgcagga gatcaccgca gccagcgagg
      421 agcaaagctc gggtgccgcg cagatcaaca ccgcgatgaa ccagctcaac cagatcaccc
      481 agcagaacgc cagtgccacg gaagaactgg cggtgacggc gcaagagatg agcgggcagg
      541 ccgaacagtt gcagcggctg atggggttct tcagggtgaa tggggcggcg agggcttaac
      601 cacaattcgt aggatgggtt gaggccttgg ccgataccca tgcggtgcat ttgatgggta
      661 tcgcgtgctc aacctatcct acgggctttc accctgaagg gtacaagagc cgcgaagctg
      721 gcgaggagat gatgcccctt gtttgagtgg tgcatctatc cctacaacag aatcagatta
      781 tccctctggg tgggggcgaa acattttgtc atcatgcgac gcgctcgatt gcggtgtcgc
      841 aaacgagatg gaaagaataa cgctgtcaag gcggcacagc ttaaggccgc agtgcaaaag
      901 aagttgaatc aatttaaaca aggaagactg ttatgcggat gaatatgccg gttacgaatg
      961 tagagcgcca tctcaaagag ggcgagtaca tcgtttcgaa gaccaatctc aaagggcaaa
     1021 tcacctatgt gaatcgcccc tttctggaga tcagcggata taccgaagaa gaactgctgg
     1081 gccagccgca caacatgatt cgtcatccgg acatgcccga gggcgctttt gccgatctgt
     1141 ggaagaccct gcagagcggc aagccctggc gcggcatggt gaagaaccgc tgcaagaacg
     1201 gcgactatta ctgggtcgat gcgaatgcca atccgttctg gaaagacggc cagatcgtcg
     1261 gtttcatgtc cttgcgcacc aagcccacgc gcgcgcaggt ggaagcggcc gaagccctct
     1321 accgcaggtt tcgcgaaggc acggcgcgcg ggctgacggt acgggaaggg gcggcggtgc
     1381 gcaccggtct gctgggaggg attgccagac tgggcaagat gggtatcaag gcgcgcgcct
     1441 ccatcgccag cgtgttgctc gtcgcaagtc tgggctggct gggttacggc gccgatgcct
     1501 cgttgcgtcc ggtcctggtc ggagcaggcg tggcattgac tgcttatatc tggtggttgc
     1561 tggcccacaa gatcttgcat cccttgcagg agggggtgcg ttcctgtcag atggtatcct
     1621 ccggcaatct gatcctggag atagaagaag aatttagcgg tgagatcggg cacctgatgc
     1681 acgcgatcaa aaccatggcg atgaatgccg agagcatcgt cgccgatgtg agctatgcct
     1741 ctgcctcgat aggcgtgtct tccagcgagg tggcaacgac cgcacagagc atgagttcgg
     1801 acatcaatgc acaggccgcc tcggtggaac agacctcggc ctcggtggaa cagatcagcg
     1861 cctccatcaa ccgcaataac gataacgcca gaagtaccgg cagcatggcg agccaggttg
     1921 ccagacaggc gatccagggc ggcgaggtgg taaaagatac cgccgccgcg atgaaacaga
     1981 tcgcgggcaa gatcggcatc atcgacgaca tcgcctacca gaccaatctg ctggcgctga
     2041 acgcggcgat cgaagccgca cgggccggag agtacggcaa gggctttgcg gtggtggcgg
     2101 ccgaagtgcg caagctggcg gagcgttcgc agttggcggc gcgggagatc ggcgaactcg
     2161 ccgctatctc ggtggacaag tccgcacagg ccggcagatt gctagacgag atcgtgccca
     2221 acatcaacaa gacttccgag ctggtgcaag agatggccac cgcatccggc gagcaagcga
     2281 ccggagccgc ccagatcaac gccgcgatga ctcagctcaa ccagatcact cagcagaacg
     2341 cgagtgccac ggaagagctg gcggccacgg cagaggcgat gagcactcag gccagaagcc
     2401 tggagcagat gatgggcttc ttcagtgtgc atgacgcggt atgccgctga cttgaccggg
     2461 gtgtggcggt ttttgcagga gcgacttaag aagagatgcg gctataccca ttgttagagt
     2521 agtgcatcta tccctacaag ataatcagag tattcctctt ggtaagggca aaacattttg
     2581 tcatcctgcg gcgtacttga ctgttgtatg gcaagcgaga tggaaagaat aacgctgcac
     2641 aggcggcaca gatcaaagtc gcagggttga aaaaaggggg gatatgagca aggtaaaggg
     2701 agcgaacggg cgtctgacgc cactgttgtg tgccttaggg tacaagggat ttccccgcaa
     2761 gcgctgctgc gtatcgaggg ggatatcaac cggcttacaa gagagttaaa catgagtttc
     2821 atgcaatttc acgcagatat acgcgatatc aacttgacct atctgatgct ggctcagcaa
     2881 atgattcggg aagaccgggc cgcagccatt tttcgtttgg ggatcagcca gaatatggtg
     2941 gacgtgatcg ataagctcac cccgcctcaa gtcatcaaga tggcctcgtc caatatattg
     3001 ttgtgccgtt tccgctttga cgaaaatctg ttgctcaaca tgttgaccga ttacagcaag
     3061 gaccgcctga tggccgatgc tcacaccacc atcatgatgg ccggacaggg tgtcgaagat
     3121 ctggttcccg agcggggcga tgcacgcaaa ctggtttttt caggaagcga gtctcggcgg
     3181 caagagttgt tgtttgcata gcgagtggtg aagggccgct cccacaaggt gcagcggttt
     3241 tcgtaggagc ggctttagtc gcgaagggga tgcggagatg caacgtgtcc atcgcggctt
     3301 aagccgctcc cacagggtgt cgcggttttc gtgagacgcc cgtcaatgaa cgcatccggc
     3361 accccgtgtg gatggaacta ctagccattc gactaggctg tcaaaagacg ccagccaagt
     3421 cactggttac cagacgacca gtgctcgggc ctgctgccga tgaaagtggg gattttctta
     3481 cccccatgtc ccttgcctac aaaaaatgaa agatttgctg atagtgaatg gcagatttct
     3541 ttgcaatgat gcgcctcgta taaattttgc gatgcaaaag atcgcaagac tgcacaggcg
     3601 acacagttca aagtcgcagg gttgataaag aatcgagaaa atatgagtga gcagaatgag
     3661 tcctgcgcgc ggcctcggcg tgtgcgcctg gggacgccgg aactgagggg aaccgggaaa
     3721 accgaactcg ccctgatcgt ggaggtgaac gaggcgatca agcaatctat cgccgtctcc
     3781 gaagggatca gcctgattgc ggtcaatgcc aatctggtgg ccggacgtgc cggtagccga
     3841 gctgccgggt tttgtatcgt ggcgggcgag ttgcgccggt tcagcgacaa catggctcac
     3901 accaaccagg ggtggtccaa gctgatcttt gagttggtgc gcgagacggc gctcaaccgc
     3961 aaccagagcc ggtattttaa tttgttgaag gcggcagcgc gtggcagcga caaggctcag
     4021 gcggccattg cagaggccag agagcgcagc ctgaacgaac tgctgattac cacggcgcac
     4081 aattcggcgc gggtgattga attgcagggc ttgatccagc gcgccgagaa gcagcaggtg
     4141 atgggggtga tgatcgcgcg ttccgcgctg atcgagtcgg cctatggcgg cgctatgcga
     4201 ccggtgttgg atcagatcgc gaccagtatc gatgccagca tcggcgattt gatttccaat
     4261 ggacgcaagg ttgagctaat gatgagcagg gtgaactaat gaaaaaaacc acggtaattt
     4321 acgcaaacgg ggaacacaaa tggctggcgg tttcgcgcga tccggagcgc cccaactacc
     4381 tgatcgacac caacgaatac ctgatcacgg atggcaagga ggcgttgctc accgatccgg
     4441 gcggctcgga aatcttcccg gcggtgtttt ccgcgctctg cgaggagttc gatccggcca
     4501 ggatcacgca tttgtttgcc tcgcaccagg acccggacat cgtgtcctcg ctggcgctgt
     4561 ggctgcaatt caatccgcag ttgcgctgct ataccagctg gttgtggacg acgttcatcc
     4621 cgcatttcgg cggcaacgac cagacgttca ttccgctccc cgacgaaggg ctgaagtttc
     4681 agctcgaccg gatcgagctg gaagcgatgc ccgcgcacta cctgcactcg tccggcaact
     4741 ttcatttgta cgatgccaag gcaaagttgc tgttcaccgg cgacatcggt gcggcgctgc
     4801 tgccggagac cgagaacgat ctgttcgtga aggatttcga caagcacatc gcttatatca
     4861 aggggtttca ccagcgctgg atgggctcga aggaggcggt gcttgactgg tgcgagcgcg
     4921 ccagcaacat ggagatcgat atgctgtgtc cgcagcacgg ttcgatctat caggggcagg
     4981 acgtgtcgcg ctttatcaac tggctgtccg agctggaagt cggcagcggg gtacgcagga
     5041 aagggtagtg gatgtacgag caggatattc gcctcaattg gcgcgccgcc atcgtattgg
     5101 ataacggcag acaggtcgtt catggccggt tgcaacgctt ggggcgcaag aaggtcgtgg
     5161 tgtgtgcgga tcacaacttg tcgccgggat atcgttgcaa tctggcggtg atgttgccga
     5221 agagcagggc agacgagacg gggattgtgg tggaggcgag tgccacgatc gccgcctcgg
     5281 tgctctccgc gatgcagttt tacatcaccc tcgaccgggt gacgtttgag ggcagagcgc
     5341 aggccctgct ggatgagcgt ctcaggattt tggggaagat gtgaaattga ttggcagagg
     5401 taacgtattg gctccaacga aacgctgatc aagttgtcat ccccgcgtag gcggggatcc
     5461 agatttttta tttaactggg ttcccgcctg cgcgggaacg acgaatgaga cttaatcaga
     5521 agctccctaa attaatgagc ggaatttaaa aatgaatgtc ttgatcgtag agccttcgcg
     5581 caccgtcgcc ttcacgctgt ctgccctgtt cgggaaatac ggtttcgagc cgcgcgtggc
     5641 cagaaacggt caggaggcgc tggatatgct gaaagtgcgt ccggcagagt tgctgtgctt
     5701 tgccttcgaa ctgggtgaca tggatggcat cgaattcttc gtgtcggcca agtcgcacaa
     5761 gctggtgcac caccagcccg ggctgatgtt cgcctccacc cagcgcaaga cgttgatcaa
     5821 ccgcgcattg atggccgggg tgacggagtg tttctccaag cgcaatctgg accagctcga
     5881 acagttcgtc gaacgctttg ccagcagcac tcatgtgcgt atcagcggaa gggtgctgct
     5941 ggtcgaggat agtccgtcgg cggccatgtt ctaccaccag atcctgaaac ggatggggct
     6001 gcatgtcgat cactgcaaga gcgcggaaga ggcgatcgag agcttcgccg tgcaggctta
     6061 cgatctggtg gtgaccgact atatcctggc cggtgcccag accggtttct cggtgatccg
     6121 cgcggtgcgc gagtcgcttg gcaagagtgc gctgacgcct attctggcga tttcttcttt
     6181 cgacgacatg gcgcgcaagg tggaaatcct gcgcaacggt gcgaacgatt tcgtcggcaa
     6241 gccggtggtg ccggaagagc tggaggtgcg tgtattcaac atgctcaaga tgcagaaact
     6301 gatgcagcgt ctggagtccc agcacgcggt gatgaaggac atcgcgatgc gcgatccgct
     6361 cacctctttg tacaaccgcc attatctgaa cgagatcacg ccgggtctga tcggcgaggc
     6421 ccatgagggc gggcagaacc tggcgctgat ggtcgtcgat gtcgatcact tcaagcagat
     6481 caacgatacc catgggcaca agcgcggaga cgaggtgttg cagcagatcg ccaagtcctt
     6541 gcagagcgtt tgtcgcagcg acgatctggt ggcccgcttc ggcggcgagg aattcgtcat
     6601 cctgcttccc ggtctcggtc tggccgaggc caccgcacgc ggcgaggcga tgcgcgcgcg
     6661 gatcgcgggg ctggaacagc cgggcggcct cgcactcacc gcgagtatcg gcattgccgc
     6721 attgatcccg ggtgaaaact atgacgaact gttccatcgc gccgacggtg cgatgtaccg
     6781 cgccaaacag ggcggtcgca accgcgtcgc ggcagcgtga gtctcaggag ctgttcgtag
     6841 gagcgccggt ttttcgtagg agcggcttga gccgcgaagg ggatgcggat gtacaacgtg
     6901 cctatcgcgg ctaaagccgc tcccacaggg tgcagcaggt tttcgcagga gcggcggatt
     6961 tcgaaggtga cggggaaatg cagcgtgttt aattaaatag ggaggtttgt aatgttcaaa
     7021 catatgaaaa tcggcatgcg tttatggttg ttggtgttgg ttgcgattac ggcgctgata
     7081 ttcacggcag ctcagtcgtt gctgaatctg cggcatagca tgctggaaga caggcaaaca
     7141 aaaacccgcc atgtggtgga aacggcttat ggcgtgatga gttattacgg cgcactttcc
     7201 gccacgcaaa aaatcagccc ggaagaagcc aggactcagg cgattaacat attgcgcagc
     7261 atgcgctacg aagagaaaga atacttctgg gtgaacacgc tggagccgcg catggtgatg
     7321 catcccatga agccggagct ggatggcaaa gatttgtcgg gtttgaaaga tcccaacggc
     7381 aagctgattt tcgtcgagtt cgccaacacg gcaaaacatg gcggcggatt tgtcgattat
     7441 ttctggccga aacccggctc cgataaaccg gtcgccaaaa tctcttacgt caagcagtac
     7501 gaaccctggg gcttagtgat cggcagcggc atttatctgg acgatgtgga tagcgcgttt
     7561 atgcgcgaag tgctgaaact cggcggtttt gccgggggcg ccttgctgat aatcggattt
     7621 tcggcctggg ttattgcccg cagcattact cgccccttga atctggcgat ggatgcagcc
     7681 aataaaattg cggcgggcga tctgtcggcc aatattcagg tcgacagcaa ggacgagacc
     7741 ggacagttgc tggtcgcgat gaagaacatg aacgacacgc tgaaaggctt gatgaccgac
     7801 accgacgtgc tggtcaaggc cgctgcggtg ggcgatctga atacccgtgc cgatgccagc
     7861 aagtatcagg gcgacttccg caagctggtg cagggcgtga acgacaccgt gaccaacatc
     7921 gccgaaccga tgaaggtcac ttccgactac atcaaccaga ttgccaaggg cgtgattcct
     7981 gcgcagatca ccaccgccta tcagggc
//
LOCUS       DASUIV010000240         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1270254, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000240 DASUIV010000000
VERSION     DASUIV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270254"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFW59_08745"
     CDS             complement(<1..672)
                     /locus_tag="VFW59_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5364347.1"
                     /translation="MIKRTRATATPFRRPTKKTEPHVIEGAKKESSAPQRLQKVLALS
                     GFGSRRDMEELIESGRVTVNGEPAKLGMTVSANDIVRIDKRAVKLQTEPQLPRVLIYH
                     KLEGEIVSQDDPEKRASVFEKLPRIRGAKWVSIGRLDINTSGLMMFTTSGELANYFMH
                     PRYEVEREYAVRVMGELSEEQMVMLTQGIELDDGIAAFDVLQDRGGEGSNHWYQVILR
                     EGRNRE"
     gene            complement(942..1487)
                     /gene="scpB"
                     /locus_tag="VFW59_08750"
     CDS             complement(942..1487)
                     /gene="scpB"
                     /locus_tag="VFW59_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5364348.1"
                     /translation="MEEPTTIQEMKNVLEAALLTSPEPLSLDDMLKLFEPRMERATLR
                     MLLDDLKHDWAERTLELVSVASGWRFQARAQYAPILARLQPEKPHRYSRAVMETLAII
                     AYKQPVTRGDIEEIRGVAVNQNVMRTLLERRWIDIVGQREVPGRPSLYGTTKTFLDDL
                     NLRTLQELPPLQEWGQHEIEF"
     gene            complement(1487..2215)
                     /locus_tag="VFW59_08755"
     CDS             complement(1487..2215)
                     /locus_tag="VFW59_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX5364349.1"
                     /translation="MPHDLYIPPEALAVYLDAFEGPLDLLLYLIRKNNLDILDIPMAA
                     LTQQYVDYIEKMQASNLELASDYLLMSAVLIEIKSRMLLPKPAEIEQEDDPRAELVRR
                     LMEYEAMKLAAQGLDAMPQVGRELMVAQAYYEHIPQQNLPGVSLDDLVAAWQGLLQRA
                     RNFQHHRITREQLSVREHMTLILRRLKLDGMVEFAALFDVTEGVQKMVTYFLALLELA
                     REGLVRITQTAGFSPIHIQATETA"
     gene            complement(2263..>3342)
                     /locus_tag="VFW59_08760"
     CDS             complement(2263..>3342)
                     /locus_tag="VFW59_08760"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5364350.1"
                     /translation="ADWHALTTHYDTPQIIEESVWEMVIDWLAAGVDPANATIFIQSR
                     VPEHAELHTLLSMITPLGWLERMPTYKDQQEKLSGKDLATYGFLGYPLLMSADVLIYK
                     ATQVPVGEDQVPHLEFTREIARRFNHIYGREPGFEEKAEAAIKKLGSKKGALYQELRT
                     RYQESGDGEALLSAQALVEEQQGLSIGDKERLLGFLEGGGKIILVEPEAKLTPASKMP
                     GLDGQKMSKSYNNTISLREEPDAVVKKIKTMPTDPARVRRTDPGEPAKCPVWQLHEVY
                     SDEQKREWVQNGCRSAGIGCIECKTPVIEAVLAELKPIQERAAQYAEDPTLVKNIVAD
                     GCEKARKLARETMREVREAMGLSYS"
BASE COUNT          540 a         1012 c         1035 g          755 t
ORIGIN      
        1 ctcgcgattg cggccctcgc gcaaaatcac ctggtaccaa tgattggagc cttcgccgcc
       61 gcgatcctgc aacacgtcga aagcggcgat gccgtcgtcc agctcgatac cctgcgtcag
      121 catcaccatt tgttcttcgg ataattcgcc catcacgcgc acggcatatt cgcgttcgac
      181 ctcatagcgc gggtgcatga agtaattggc gagttcgccc gaggtggtaa acatcatcag
      241 gccggaggtg ttgatgtcca gccgcccgat ggaaacccat ttcgcgcctc tgatgcgcgg
      301 cagcttctcg aacaccgacg cgcgcttttc cgggtcgtcc tggctgacga tttctccttc
      361 cagtttgtgg tagatcagca cgcgcggcag ttgcggctcg gtttgcagtt ttaccgcgcg
      421 cttgtcgatg cgcacgatgt cgttggcgga gaccgtcatg ccgagcttgg ccggttcgcc
      481 gttgaccgtg acgcgaccgg attcgataag ttcctccatg tcgcggcgcg agccgaagcc
      541 cgacagtgcc aacacctttt gcaggcgctg cggcgcggaa ctttctttct tcgctccctc
      601 gatgacatgg ggttcggtct ttttggtcgg ccgcctgaac ggggtcgctg tggctcgtgt
      661 gcgttttatc ataatagggc ttgttcatgc tgatttcgcg tctgcgacgg gggcgatttg
      721 ggatgccatc cgcgaagcaa gacgcgtagt tgtgccgccc acaataagcg gcttgcgaca
      781 aaggagggca cccaaaacgc ccccgtcccg tagggttgcc aagataacac gcgcctgcgg
      841 cgttgcgaag cttgcgaatg gaatgccatt cgctgcactc cgcgccttgc attcatcgtg
      901 ttatcttggc aacgcaggcg tgaaatcagc atgaacaagc cctaaaattc tatctcgtgt
      961 tggccccatt cctgcaatgg cggcagttcc tgcaacgtcc tcaagttgag gtcgtcgaga
     1021 aaggttttag tggtgccgta cagcgatggc ctgcccggca cttcgcgttg tccgacaatg
     1081 tcgatccagc gccgctcaag cagcgtgcgc atgacgttct ggttgaccgc gacaccgcgt
     1141 atttcctcga tgtcgccgcg cgtcaccggc tgtttgtagg cgatgatcgc cagcgtttcc
     1201 atcacggcgc gcgaatagcg gtgcggcttt tccggctgta ggcgggcgag gatgggcgcg
     1261 tattgcgcgc gcgcctgaaa gcgccagccg ctcgccacgg ataccagttc cagcgtgcgc
     1321 tccgcccaat cgtgcttgag gtcgtccagc agcatgcgca acgtggcgcg ctccatgcgc
     1381 ggctcgaaca gcttgagcat gtcgtccagc gacagcggct ccgggctggt gagcagagcc
     1441 gcttccagca cgtttttcat ctcctgtatg gtggtaggtt cttccatcat gcggtttccg
     1501 tggcctggat atggatgggc gagaacccgg ccgtctgcgt gattcttacc agtccttcgc
     1561 gcgccagttc gagcagcgcg agaaaatagg tcaccatctt ttgcacgcct tccgtcacgt
     1621 cgaacaacgc ggcgaattcc accatgccat cgagcttgag acggcgcagt atcagcgtca
     1681 tatgctcgcg caccgacagt tgctcgcgcg tgatgcgatg gtgctggaaa ttgcgggcgc
     1741 gttgcagcaa tccctgccac gccgcaacca ggtcgtccag gctgacgccg ggcaggttct
     1801 gctgcggaat atgctcgtag taggcctgcg ccaccatgag ttcgcgtccc acctgcggca
     1861 tggcatccag tccttgtgcc gccagcttca tggcttcgta ttccatcagg cgccgcacca
     1921 gttcggcacg cgggtcgtct tcttgctcga tctcggccgg cttcggcagc agcatgcgcg
     1981 acttgatctc gatcagcacc gccgacatca gcaggtaatc ggacgccagc tccagattgc
     2041 tggcctgcat cttctcgatg tagtccacgt attgctgcgt aagcgcagcc atggggatgt
     2101 cgaggatgtc caggttgttc ttgcggatca ggtacagcag caggtcgagc ggcccttcaa
     2161 acgcgtcgag atatacagcc agcgcctcgg gcgggatgta caggtcgtgc ggcatctcgg
     2221 cgagcggttc gccgtggatg cgcgcactgg cctgtggggc aatcacgagt aggagagtcc
     2281 catcgcctcg cgcacttcgc gcatggtttc gcgagccagc ttacgtgcct tttcgcagcc
     2341 gtcggcgacg atgttcttca ccagcgtcgg gtcttcggcg tattgcgcgg cgcgctcctg
     2401 gatcggcttg agttcggcca gcacggcctc gatcacgggc gtcttgcact cgatgcagcc
     2461 gatgcccgcg ctgcggcagc cgttttgcac ccattcgcgc ttctgctcgt cggagtagac
     2521 ttcgtgcagt tgccagaccg ggcacttggc cggttcgccc gggtcggtgc ggcgcacgcg
     2581 agccgggtcg gtgggcatgg tcttgatttt cttgaccact gcgtccggtt cctcgcgcag
     2641 cgagatggtg ttgttgtagg acttggacat tttctgcccg tccagccccg gcatcttgga
     2701 ggccggggtg agcttggctt ccggctcgac caggatgatc ttgccgccgc cttcgaggaa
     2761 gcccagcagg cgttccttgt cgccgatgct caagccttgt tgttcctcaa ccagcgcttg
     2821 cgccgatagc agcgcttcgc cgtcgccgga ttcctggtag cgggtgcgta gttcctgata
     2881 cagcgcgcct ttcttgctgc ccagcttttt gatcgcggct tcggcctttt cctcgaagcc
     2941 gggttcgcgg ccgtagatgt ggttgaagcg gcgagcgatc tcgcgggtga attccaggtg
     3001 aggaacctga tcttcgccca ccggtacctg ggtggccttg tagatcagta cgtcggcgct
     3061 catcagcaag gggtagccaa gaaagccgta ggttgcgagg tctttgccgg aaagtttttc
     3121 ctgttggtcc ttgtaggtcg gcatccgctc cagccagccc agcggggtaa tcatcgacag
     3181 cagggtgtgc agttcggcat gctccggcac gcgcgactgg atgaagatgg tggcattggc
     3241 gggatcgacc ccggcggcga gccagtcgat gaccatttcc cacacgcttt cctcgatgat
     3301 ctgcggggtg tcgtagtggg tggtcagcgc atgccagtcg gc
//
LOCUS       DASUIV010000241         4539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_60553, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000241 DASUIV010000000
VERSION     DASUIV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60553"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..867)
                     /gene="pth"
                     /locus_tag="VFW59_08765"
     CDS             complement(292..867)
                     /gene="pth"
                     /locus_tag="VFW59_08765"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5364351.1"
                     /translation="MSQIRLIVGLGNPGREYENTRHNAGFWWVDEFAREHGLNFRSEA
                     KFHGQSVRGNPQGHEIFLLKPQTFMNVSGRAVGALAQFYKIAPAEILVVHDELDLPPG
                     SAKLKLGGGHGGHNGLKDIIAHLGSRDFWRLRLGVGHPGERSEVSGYVLNDPRREERA
                     LVDEAMQRSLAVAHLIIEGKTEAAMLKLHSN"
     gene            complement(952..1587)
                     /locus_tag="VFW59_08770"
     CDS             complement(952..1587)
                     /locus_tag="VFW59_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX5364352.1"
                     /translation="MTIEMNVTNRKTQGTGASRRLRHAGRVPGIIYGSGDAISIELDH
                     NEIYHKLRSEAFHASVLTLNVEGKKDTVLLRDFQMHPFRQQVMHIDFQRVDPKQKMHI
                     KVPLHFLNEEVAPGVKTGGGKVSHVMTELDVTCLPKDLPAFIEVDLGKLELGHSVHVS
                     DLKLPKGVTVSAHGTHTAEAVIATVQVPRGTIEAAAEVAAEAAPAAPAAKK"
     gene            complement(1683..2633)
                     /locus_tag="VFW59_08775"
     CDS             complement(1683..2633)
                     /locus_tag="VFW59_08775"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5364353.1"
                     /translation="MSYDSLMVFTGNANPKLAEAVAQHLKISLGRATVGRFSDGEVMV
                     ELLENVRGKDVFVLQSTCVPTNDNLMEVMVMVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVAITAKVVADMLSSAGVNRVLTMDLHSDQIQGFFDVPVDNIYASPILLADVRK
                     QKYPNLIIVSPDVGGVVRARALAKELDADLAIIDKRRPKPNVAKVMNIIGDVVGRTCL
                     IMDDMVDTANTLCEAANALKEKGASRVLAYCTHPVLSGPAIERIENSAIDELVVTDTI
                     PLSEAARNCSRIRQLSTAELLAETIRRINNEESVSSLFTE"
     gene            complement(2787..2861)
                     /locus_tag="VFW59_08780"
     tRNA            complement(2787..2861)
                     /locus_tag="VFW59_08780"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2827..2829),aa:Gln,seq:ttg)
     gene            complement(2873..3703)
                     /gene="ispE"
                     /locus_tag="VFW59_08785"
     CDS             complement(2873..3703)
                     /gene="ispE"
                     /locus_tag="VFW59_08785"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX5364354.1"
                     /translation="MKLTCPAPAKLNLFLHVVGRRADGYHLLQTLFRFIDLQDTLHFE
                     PRDDGEVHRTNRVEGVPEEQDLCVRAARLLQAETGCALGVDISIEKRIPMGGGLGGGS
                     SDAATTLLALNRLWSLGLSRERLMQLGLQLGADVPVFVFGENAFAEGVGEALQAYSLP
                     EAWYLVLFPPVQVPTEKIFTHPELTRDSVPIIIRALLKQSLRNDLQSVACKLYPEVAR
                     HIDWLSKYGRAMMTGSGACVFAEFDSREQAEAVLRQLPEDMRGVVAQGLEKHPLHDWV
                     "
     gene            complement(3703..4266)
                     /gene="lolB"
                     /locus_tag="VFW59_08790"
     CDS             complement(3703..4266)
                     /gene="lolB"
                     /locus_tag="VFW59_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEX5364355.1"
                     /translation="MIRGLLSLWLILLGGCTLMPSTHAPVARPVQSEVAPFALNGRVA
                     INHNGTHHSAGLRWEHQPASDELLLLGPLGQTGARVYRDARGATLDDGSKHYQAEDVE
                     SLMQQVLGWHLPLSGLHQWVLGLAVEGEAQIERDKQGRIGVLRQSGWEVRYLRYVDDR
                     ADSLPARLQLSRDGLQVQLLIDEWEWQ"
     gene            complement(4263..>4539)
                     /locus_tag="VFW59_08795"
     CDS             complement(4263..>4539)
                     /locus_tag="VFW59_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5364356.1"
                     /translation="VEKALKLAPDDAAIMDSVGWGYYRNGRLDDSVNMLRRAFASNPD
                     PEIAAHLGEVLWVRGDKDEARALLQDSLKSNPDNAPLQAVIKKFIFR"
BASE COUNT          946 a         1482 c         1195 g          916 t
ORIGIN      
        1 acgatcatca attcgatcag ggtaaaacct ttttgtacgt ttttcatgtg aagctccttg
       61 tagagttaac gttgtctaac cgctcgatcc ggttgccaag cctggccaac cttgccggtg
      121 gttaacacac atcgtgtgtc gctggtctat gcagcaatag ccgtgccaga gttgcaagcg
      181 attgttttat tggagccggg aaaaataccc ccgtccccga aacccaaaac ccaacaaaat
      241 ccgtccatcg actgacgact ttcgtcatcc ggatttcctt agccctgaat cctaattact
      301 atgcagcttc agcatcgccg cctcggtctt gccttcgata atcagatgcg cgaccgccag
      361 cgaacgctgc atggcctcgt cgaccaacgc ccgctcttcc cggcgcggat cgttcagcac
      421 atagccggac acctcgctgc gctcgccggg atgaccgacg ccgagacgca gccgccagaa
      481 atcgcgtgaa cccagatggg cgatgatgtc cttcaggccg ttgtgaccgc cgtgtccgcc
      541 gcccagcttg agcttggcgc tgcccggcgg cagatccagc tcgtcatgca ccaccaggat
      601 ctccgccgga gcgatcttgt agaactgcgc cagtgcgccg acggcacgcc cgctcacgtt
      661 catgaaagtc tgcggcttga gcagaaatat ctcgtgcccc tgcggattgc cgcgcaccga
      721 ctgaccgtga aacttggcct cgctcctgaa attcaggcca tgctcacggg cgaattcatc
      781 cacccaccag aaacctgcgt tgtggcgagt gttctcatat tcacggccgg ggttacccag
      841 cccgacgatc aagcgtatct gactcatggc gcgtcaactc atcatgccta aaataaaaaa
      901 cccgccatgc cgaaatcagc aatggcgggt tagcagggat tgaaccctcg attacttctt
      961 ggcggcgggt gcggccggag cagcttcggc tgcaacttca gcagccgctt cgatcgtgcc
     1021 gcgcggcacc tgcaccgtcg cgatcacggc ttctgcggtg tgagtgccgt gtgctgaaac
     1081 ggtcacgccc ttcggcagtt tcaggtcgga aacgtgcacc gaatggccca gctccagctt
     1141 gcccagatcc acttcgatga atgccggcag atccttcggc agacaggtca catccagctc
     1201 ggtcatcacg tggctgacct tgccgccacc ggttttcacg cctggcgcca cttcttcgtt
     1261 gaggaagtgc aacggcacct tgatgtgcat cttctgcttc ggatccacgc gctggaagtc
     1321 gatatgcatc acttgctgac ggaacgggtg catctggaaa tcgcgcaaca gcacggtgtc
     1381 cttcttgcct tcgacgttca gtgtcaacac ggaagcatgg aacgcttcgg aacgcagctt
     1441 gtggtagatc tcgttatgat ccagttcgat cgagattgca tcaccggagc cgtagatgat
     1501 gccgggcacg cgcccagcat gacgcagacg gcggctcgca cccgtacctt gcgttttgcg
     1561 attcgttaca ttcatttcaa tggtcatttt acttctccaa aaacagaacc ctccgcgacc
     1621 agagggttct tactttgcgg cagtccccat gaccgccgct tatgctgctc gatgagtgcc
     1681 gtttactcag taaacagcga actcaccgat tcttcgttat tgatacgtcg tatcgtttcc
     1741 gccagcagct ccgccgtact cagttgccgg atgcggctgc agttacgcgc cgcctcgctc
     1801 aacggaatgg tatcggtaac caccagctcg tctatcgccg aattctcgat gcgctcgatg
     1861 gcaggaccgg acagcaccgg gtgggtacag taagccagca cacgtgacgc gcccttctcc
     1921 ttcagcgcat tcgccgcttc gcacagcgtg ttggcagtgt ccaccatgtc atccatgatc
     1981 aggcaggtgc gcccgacgac gtcgccgatg atgttcatca ccttggccac gttcggcttg
     2041 gggcgacgct tgtcgatgat cgccagatcc gcatccagct ccttggccag cgcacgggca
     2101 cgcaccacgc cgcccacgtc cggcgacacg atgatcagat tcgggtattt ttgcttgcgc
     2161 acatcggcca acaggatcgg gctggcgtag atgttatcta ccggcacgtc gaagaacccc
     2221 tgaatctggt cggagtgcaa atccatggtc agcacgcgat tgacgcctgc gctggagagc
     2281 atatccgcca ccacttttgc ggtgatcgcg acacgcgccg aacgggggcg cctatcctgg
     2341 cgcgcataac cgaaataggg aatcgctgca gtgatgcggc cggcagaagc gcgcttgagc
     2401 gcatccacca tcaccatcac ttccatcaga ttatcgttgg tcggaacaca ggtcgattgc
     2461 aacacgaaca cgtctttgcc gcgcacgttc tcgagcaact caaccatgac ctcgccgtcg
     2521 gaaaaacggc ctaccgtggc gcgtccaaga ctgattttga ggtgctgcgc gactgcttct
     2581 gcgagcttag gattggcatt gccggtgaat accatcaagc tgtcgtagga cacgtcaacc
     2641 ccttaataga gactagggca tctgcccaaa taagtgttcg gccactcgcc taggcgagct
     2701 tgacgttcgc ttcgctcacg tcggcctgca ccgaaactgc gttttggctt cgtccgcacg
     2761 tcttaatgta cgccgaaacc gtattctggc tggggaggta gggatcgaac ctacgaatgc
     2821 cggaatcaaa atccggtgcc ttaccacttg gcgactcccc aatgaactct tgctacaccc
     2881 aatcatgcaa cgggtgcttc tccaaaccct gcgctactac accgcgcata tcctccggca
     2941 actgccgaag taccgcctct gcctgctcgc gactgtcaaa ctccgcgaat acgcaggctc
     3001 ccgatccggt catcatcgcc ctgccgtatt tgctcagcca atctatatgc cgagccactt
     3061 ccgggtagag cttgcatgcc actgattgca gatcgtttcg caggctctgc ttcaagaggg
     3121 cgcgaattat gatgggaacc gaatcgcgtg tcaattccgg atgcgtaaat attttttcgg
     3181 tcggcacttg cacgggcgga aaaagcacga gataccaagc ttccggcagg gagtaagcct
     3241 gcaacgcctc ccccacaccc tcggcaaaag cattctcacc gaacacgaat accggcacat
     3301 ccgcccccag ctgcaggcca agctgcatca ggcgctcgcg cgacaagccg agcgaccaca
     3361 gacgattcaa cgcgagcagt gtcgtggccg catccgaact gccgccgccc aagccgccgc
     3421 ccatcggaat gcgcttctcg atagagatat cgacccctag cgcgcatccc gtctccgcct
     3481 gcaacaggcg cgcggcgcgc acgcaaagat cctgctcttc cggcacgccc tcgacacgat
     3541 tggtgcgatg cacctcgccg tcgtcgcgcg gctcgaagtg cagcgtgtcc tgcaaatcga
     3601 tgaaacggaa cagggtctgc aacagatggt aaccatcagc cctgcgaccg accacatgca
     3661 aaaaaagatt gagcttcgcc ggagcggggc aagtcagctt catcattgcc attcccattc
     3721 gtcgatcagc aactgcacct gcaaaccgtc gcgactcaac tgcaagcgcg cgggcagact
     3781 gtcggcacgg tcgtcgacgt agcgcagata gcgcacctcc cagccagact ggcgcagcac
     3841 gccgatgcgc ccctgcttgt cgcgctcgat ctgcgcctcg ccctccactg ccaagcccaa
     3901 cacccattga tgcagaccgc tcagtggcag atgccagccc agcacctgct gcattagcga
     3961 ctcgacatcc tccgcctgat aatgtttgct gccgtcatcc agtgtcgcgc cgcgagcatc
     4021 ccggtagacg cgcgcacctg tctgacccag cggcccaagc aacaacaact cgtcggatgc
     4081 cggctgatgc tcccagcgca gcccggccga atgatgcgtg ccgttatgat tgatcgcaac
     4141 gcgtccgttc aacgcgaagg gggcgacctc cgactgaacc ggacgcgcca caggcgcgtg
     4201 cgtgctcggc atcaacgtac aaccgcccag caggatcagc cagagcgata acaggccacg
     4261 aatcatcgga aaatgaactt cttgatcacc gcctgcaacg gagcgttgtc cggattcgac
     4321 ttgagactgt cttgcaacag cgctcgcgcc tcatccttgt cgccgcgcac ccacagcacc
     4381 tcgcccagat gcgccgcgat ctcgggatcg ggattgctgg caaaggcgcg acgcagcatg
     4441 ttaacgctgt catccaggcg cccgttgcgg taataacccc aacccacgct atccatgatg
     4501 gccgcatcat ccggcgcaag cttcagcgcc ttttccacc
//
LOCUS       DASUIV010000242         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1178318, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000242 DASUIV010000000
VERSION     DASUIV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178318"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFW59_08800"
     CDS             complement(<1..632)
                     /locus_tag="VFW59_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5364357.1"
                     /translation="MKVGLIGWRGMVGSVLMQRMREEKDFDLIEAVFFTTSNVGGAAP
                     AEGRGIPLKDAFDLNELKAMDAIISCQGGDYTTDVFPRLRAAGWQGYWIDAASTLRME
                     NDAVIILDPVNLDLIKASLSSGVKNYVGGNCTNSILLMGLGGLFHAGLVEWVSSMTYQ
                     AASGAGAQNMRELLNQMGVVHASVADLLANPQSAILEIDRRVAAALRSFE"
     gene            complement(726..1790)
                     /gene="leuB"
                     /locus_tag="VFW59_08805"
     CDS             complement(726..1790)
                     /gene="leuB"
                     /locus_tag="VFW59_08805"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5364358.1"
                     /translation="MKIAVLPGDGIGPEIVAQAVKVLEALRSDGLSIEMEYAHIGGAG
                     YDAAQDPLPEATLKLAKASDAVLLGAVGGYQYDNLPRPMRPEQGLLRIRKGLGLFANL
                     RPAMVYPELVNASSLKPELVSGLDIMILRELTGDIYFGQPRGIRELPNGERQGFDTMH
                     YSESEIRRVAHVGFQSAMKRGKKLCSVDKANVLDTSMFWREIVTEVGREYPEVELSHM
                     YVDNAAMQLVRAPKQFDVILTGNIFGDILSDEASMLTGSIGMLPSASLDANNKGLYEP
                     CHGSAPDIAGKDIANPLATILSVAMMMRYTFEREDVAQRIEAAVRKVLQQGLRTGDIA
                     EAGTRKVGTAAMGDAVVAAL"
     gene            complement(1884..>2124)
                     /locus_tag="VFW59_08810"
     CDS             complement(1884..>2124)
                     /locus_tag="VFW59_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5364359.1"
                     /translation="KVDALFKAVAVNSGYKLRVDLEQQTIALPDGTTYQFDVDAHRKH
                     CLLNGLDDIGLTLQHVAEIKAFEEKHRAAQPWLYA"
BASE COUNT          397 a          710 c          587 g          430 t
ORIGIN      
        1 atctcaaacg agcgcagcgc tgcggccacg cgccggtcga tttccagtat cgccgactgg
       61 ggattggcca gcaggtcggc cacagaagcg tgaaccaccc ccatctggtt cagcagttct
      121 cgcatattct gggctcccgc accggatgcc gcctgatagg tcatgctcga cacccactcc
      181 accagcccgg cgtggaacag accacccagc cccatcagca agatgctgtt ggtgcagttg
      241 ccgccgacgt agttcttgac gccgctcgac agcgacgctt tgatgagatc gagattgacc
      301 ggatcgagaa tgatgaccgc gtcgttttcc atgcgcaagg tggaggccgc atcaatccag
      361 tagccttgcc agcccgccgc gcgcagtctg gggaagacat cagtggtgta gtcgccgccc
      421 tggcaggaaa tgatcgcgtc catcgccttc agctcattca ggtcaaacgc atccttgagc
      481 ggaatgccgc gcccctctgc aggtgctgcg cctccgacat tggaagtggt gaagaacacc
      541 gcctcgatca gatcgaaatc cttttcctcg cgcatgcgct gcatcagaac cgaccccacc
      601 ataccgcgcc aaccgatcaa tcctactttc attctgattc cttcattttc ttaatgggga
      661 agggtacgcg gctgcgatgc caaggcaaaa gccgccgtac cgcttccacc cttccccttt
      721 ttgttttaca gcgccgcgac caccgcgtcg cccatcgccg ccgtacccac cttccgagta
      781 ccggcctcgg cgatatcgcc ggtgcgcagg ccttgctgca acactttacg cacagcggcc
      841 tcaatgcgct gcgcgacgtc ttcgcgctcg aaggtatagc gcatcatcat cgccaccgac
      901 aggatggtcg ccaacggatt ggcgatatcc ttgcccgcga tgtccggcgc ggagccgtgg
      961 cagggctcgt acagcccctt gttgttcgca tccagcgaag ccgaaggcag catgccgatc
     1021 gaaccggtca gcatcgaagc ctcgtcggaa agaatatcgc cgaagatgtt gccggtcagg
     1081 atcacgtcga actgcttcgg tgcacgcacc agctgcatcg cggcgttgtc cacatacatg
     1141 tgcgacagtt cgacttccgg gtattcgcgg cccacttcgg tgacgatctc gcgccagaac
     1201 atgctggtgt ccagcacgtt ggccttgtcc accgagcaaa gcttcttgcc gcgcttcatc
     1261 gcgctctgga aaccgacatg cgcgacgcgg cggatctcgg actcgctata gtgcatggtg
     1321 tcgaagccct ggcgctcgcc gttcggaagc tcgcggatgc cgcgcggctg accgaagtag
     1381 atgtcgccgg tgagttcgcg caggatcatg atgtccagcc cggacaccag ctccggcttc
     1441 aggctcgatg cattgaccag ctcggggtaa accatcgccg gacgcaggtt ggcgaacagc
     1501 cccagcccct tgcggatccg cagcaagccc tgttcgggtc gcatcgggcg tggcaggttg
     1561 tcgtattgat agccgcccac cgcacccagc agcaccgcat cggaggcttt agccagcttc
     1621 agcgtcgctt ccggcagtgg gtcctgcgcc gcgtcgtagc ccgcgccgcc gatatgtgca
     1681 tattccatct cgatgctcag accgtcgctg cgcaacgctt ccaacacctt caccgcttgc
     1741 gcgacgatct ccggtccgat gccgtcaccc ggcaaaactg cgattttcat ttcaaattcc
     1801 cattttccat aaactaaaat caaatccccc gctacgccgc cccctttgac aaagggggct
     1861 atggggaatt tacggcttgc ttgctaggcg tacagccagg gctgcgccgc gcggtgcttt
     1921 tcctcgaacg ccttgatctc ggccacatgc tgcagcgtca ggccgatgtc gtccagcccg
     1981 ttcagcagac agtgcttgcg gtgcgcatcc acgtcgaact ggtatgtcgt accgtccggc
     2041 agcgcgatag tctgctgctc cagatcgacc ctcagcttgt agccgctgtt gactgcaacc
     2101 gccttgaaca gcgcgtcgac cttg
//
LOCUS       DASUIV010000243         8736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1178695, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000243 DASUIV010000000
VERSION     DASUIV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178695"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFW59_08815"
     CDS             complement(<1..707)
                     /locus_tag="VFW59_08815"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987242.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX5364360.1"
                     /translation="MLSPITEQDFKNLAAQGYNRIPLVAETFADLDTPLSLYLKLANK
                     PYSYLLESVQGGERFGRYSFIGLPADTRITVRGKRITLTHKNDETTLEADNPLDFISE
                     YQSRFKVAPLSGLPRFTGGLAGYFGYDTVRYIEPRLAPENNKNISAKPDMIGTPDILL
                     MLTEQLAVIDNLSGKLTFIVYADPSQDDAYALARERLRELIGMMRLPVEIPYAPATPR
                     VEAKSEFGEAAFKQAVE"
     gene            complement(825..1520)
                     /locus_tag="VFW59_08820"
     CDS             complement(825..1520)
                     /locus_tag="VFW59_08820"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX5364361.1"
                     /translation="MQPNIRFTPPLNVQAIVIDLDGTLLHTAPELSEAANRMLREMDY
                     APVSQELLASYIGNGISWLVKRALTGDMHATPDAALYDHALPIFEKHYTDLLLQSRVF
                     DGVIEGLEAMKAAGYRLGCITNKVERYTTPLLEGIGLAKYFEIVLAGDTLPEKKPHPM
                     PLLHAAKFFGVPIEQLLLIGDSLNDAIAARAAGCPIFCVPYGYNHGEPVETLDLDAVI
                     ANLPAALPLIKRA"
     gene            complement(1525..1917)
                     /locus_tag="VFW59_08825"
     CDS             complement(1525..1917)
                     /locus_tag="VFW59_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364362.1"
                     /translation="MHSLRSLLLLAALALAGCDRISGEADKKIYDAEAIGYACRVSLK
                     APEDCMKENETQSPTSVLDGWKAANKDIEKGVIDPTMGKRPASVIHAPQSAVAETATN
                     ADTVKMALPDLKAVAKAGTATTEKPAKH"
     gene            complement(1926..2606)
                     /gene="rpe"
                     /locus_tag="VFW59_08830"
     CDS             complement(1926..2606)
                     /gene="rpe"
                     /locus_tag="VFW59_08830"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX5364363.1"
                     /translation="MFQIAPSILSANFAKLGEEVDNILASGADIVHFDVMDNHYVPNL
                     TIGPLVCEALRKHGVTAPIDVHLMVKPVDRIIPDFAKAGATYITFHPEASEHIDRTIG
                     LIKELGCKAGLVFNPSTPLDILEYTLPKLDMVLLMSVNPGFGGQKFIPHVLDKARKVR
                     AMIDAGGYNCRLEIDGGVGPANILEVAKAGVDTFVAGSAIFGAGKDSDPHRYDSVVAA
                     MRAELAKA"
     gene            complement(2744..3457)
                     /locus_tag="VFW59_08835"
     CDS             complement(2744..3457)
                     /locus_tag="VFW59_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5364364.1"
                     /translation="MTIKAIIFDVDGTLADTEDAHRKSFNKAFAENGLDWNWDVALYD
                     KLLRVTGGKERIKYFVDSFLQGFAKPADYEDFVKHLHKVKTAHYVAMLREGLIPLRPG
                     VRQLIDDARNAGIALAISTTTSPENVAGLLEVGIGRDWADYFAANGCGDIVPHKKPAP
                     DIYFWVLDKLGIPATDCIALEDSENGLRSSLAAGIKTYVTLNHYTRNQDFSGAAGVFN
                     DLSDLGNFYRATGLPLPTA"
     gene            complement(3487..4836)
                     /gene="ffh"
                     /locus_tag="VFW59_08840"
     CDS             complement(3487..4836)
                     /gene="ffh"
                     /locus_tag="VFW59_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5364365.1"
                     /translation="MLDNLTQRLSGVIKNLRGQSRLSESNIQDALREVRMALLEADVA
                     LPVVKDFIAQVKQAALGAEVVGNLNPGQALIGVVHRELTKLMGEHNDALNLATTPPAV
                     ILMAGLQGAGKTTTSGKLAKLLRDQQKKKVLLVSCDVYRPAAIEQLRTLAQQLEIDFF
                     ASDISQKPKDIALAALDYARRHYHDVLIVDTAGRLAIDTAMMEEIQQLHAALKPIETL
                     FVVDAMTGQDAVNTARAFGETLPLTGVILTKLDGDARGGAALSVRQITGKPIKFVGVS
                     EKPNGLEAFHPERMASRVLGMGDVLSLIEEAQRGVNMAEAEKLAKKVQKGEGFDLNDF
                     KMQIVQMRNMGGMSALMDKLPAQLSQAAAGAKVDDKQINRIEGIINSMTPLERSKPEL
                     IKASRKRRIAMGAGVQVMHVNQLLNQFEQSQKMMKMFSKGGMGKLMRGMAGKFPGLR"
     gene            4891..5703
                     /gene="ccsA"
                     /locus_tag="VFW59_08845"
     CDS             4891..5703
                     /gene="ccsA"
                     /locus_tag="VFW59_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5364366.1"
                     /translation="MSNVILSWLAFTIYSVLAVYFWQAQVRGKGDALIRGAAGHWVLV
                     PLALQGYLLVQDVFAGGGFNLSIANALSLIVWLTLGVYWVARFFYPVGGLQALVLPLA
                     AVAVLLPEIFPTSHQLENTGLLAFKAHIAAAMLAYSLFTIAMLHAVLISQVEKRLHHA
                     TLPRVLQNLPPLLTMETLLFRIIGIGFLLLTLTLASGVVFSEAIFGKAWQLNHKVLFG
                     FISWLVFAVLLWGHRFHGWRGRIAVRWTVSGFVLLLLAYLGSKFVIEVLLHR"
     gene            5806..6057
                     /gene="rpsP"
                     /locus_tag="VFW59_08850"
     CDS             5806..6057
                     /gene="rpsP"
                     /locus_tag="VFW59_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5364367.1"
                     /translation="MVIIRLSRGGSKNRPFFNVVVADSRNRRDGRFIERVGFYNPIAA
                     EGQEALRLQMERVSFWQSKGAQLSDAVAKLVKRAGVAAA"
     gene            6092..6592
                     /gene="rimM"
                     /locus_tag="VFW59_08855"
     CDS             6092..6592
                     /gene="rimM"
                     /locus_tag="VFW59_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030861.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5364368.1"
                     /translation="MVIMGRVAAAHGIRGWVKIQPFTEYLDSLLEYGTWWLGHEQGPW
                     REVKVQQSEVHHKTLAALLENCPDRTAAEKLKGLLIAVPRSSLPEQDEDEYYWSDLIG
                     MSVVNEAGEILGVVENLLETGANDVLSVKGSSGSILIPFVDSAVKQVDVASRTIRVDW
                     SADYLS"
     gene            6589..7359
                     /gene="trmD"
                     /locus_tag="VFW59_08860"
     CDS             6589..7359
                     /gene="trmD"
                     /locus_tag="VFW59_08860"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5364369.1"
                     /translation="MIFDVITLFPQMFEALTQYGITRRAAEQGKYVLKTWNPRDFTTD
                     NYRTIDDRPYGGGPGMVMLGEPLAGAINAAKQRQAASGVKKSRVVYLSPQGRLLTHGV
                     VKQLVAQQDEGLILLAGRYEGIDERLIRQYVDEEISIGDYVLSGGELAAMVLVDSLVR
                     QLPGVLGDAESAEQDSFVQGLLDCPHYTRPEEFNGEAVPPVLLSGNHADIQRWRLQQS
                     LGRTWLRRPDLLARRDLTKEESRLLAQFQKEQDPITKE"
     gene            7364..7726
                     /gene="rplS"
                     /locus_tag="VFW59_08865"
     CDS             7364..7726
                     /gene="rplS"
                     /locus_tag="VFW59_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX5364370.1"
                     /translation="MNLIEQLEQEEIARLGKTIPVFAPGDTVVVGVNVVEGERKRVQV
                     FEGVVIAKRNRGLNSAFIVRKISAGEGVERTFQTYSPIIASIEVKRKGAVRRAKLYYL
                     RDRSGKSARIKEKLAARG"
     gene            7843..8325
                     /locus_tag="VFW59_08870"
     CDS             7843..8325
                     /locus_tag="VFW59_08870"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5364371.1"
                     /translation="MRYHRTMDYQAKLRVPFGVLGIRCSADALVGIDFLAVSESAQTP
                     TGEIAAMACEQLRGYFDDPQASLTVPLDLRGTAHRHRVWQAILHIPCGQTRSYGELAA
                     ALHSGAQAVGQACGVNPVPIVVPCHRVIAKAGLGGFMQSAGGATLDIKRWLLKHEGVL
                     "
     gene            8322..>8736
                     /locus_tag="VFW59_08875"
     CDS             8322..>8736
                     /locus_tag="VFW59_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5364372.1"
                     /translation="MNNADLLDEFCDNLWLEDGLSRNTLDSYRRDLRKFGGWLEKQRE
                     ASVLQAAHGDIQGYLAHLVVEQKAKPSSTGRNISSLKRLFRYLLRQGKIAVDPTLQID
                     TPRLPRNLPKSLTEQDVELLLDAPDMQTPLGLRDRT"
BASE COUNT         1561 a         2395 c         2822 g         1958 t
ORIGIN      
        1 ttctccaccg cctgcttgaa cgcggcttcg ccgaattcgg acttggcctc gacgcgcggt
       61 gtggccggcg catagggtat ctcgacgggc agccgcatca tgccgatgag ttcgcgcagc
      121 cgctcgcgcg ccagcgcgta ggcatcgtcc tgcgaagggt ctgcatagac gatgaaggtg
      181 agcttgccgg acaggttgtc gatcaccgcc agctgctcgg tgagcatcag caggatgtcg
      241 ggcgtgccga tcatgtccgg tttggcggag atgtttttat tgttttccgg ggccaggcgc
      301 ggctcgatgt agcgcaccgt gtcgtagccg aagtagccgg ccagtccgcc ggtgaagcgc
      361 ggcaggccgg acagcggcgc gaccttgaaa cgcgactggt attcgctgat gaaatccagc
      421 gggttgtcgg cctcgagggt ggtctcgtcg ttcttgtgcg tcagcgtgat ccgcttgccg
      481 cgcaccgtga tgcgcgtgtc ggcgggcagg ccgatgaagg aatagcgccc gaaacgttcg
      541 ccgccctgca ccgattccag caggtaggaa tagggcttgt tggcgagttt caggtagagc
      601 gacagcggcg tgtcgagatc ggcaaaagtc tcggcgacca gcgggatgcg gttgtagccc
      661 tgcgctgcaa ggttcttgaa atcctgttcg gtgatcgggg acaacataac aactccttaa
      721 aaattatttc gggtacgagg gatggcgatg catgcggtga ggcatgcaat caccatcgcc
      781 agctgctcgt gaccgaaatc tggagtcgtt gatgtgtcat ggcgtcaggc gcgtttgatc
      841 agcggcaacg cggccggcag gttggcaatc accgcgtcga gatccagcgt ctccaccggc
      901 tcgccgtggt tgtagccgta gggtacacag aagatcgggc agcctgccgc gcgcgccgcg
      961 atggcgtcgt tcagcgaatc gccgatcagc agcagctgtt cgatcggcac gccgaagaat
     1021 tttgcggcat gcagcaacgg catcggatga ggcttcttct ccggcagcgt gtcgccggcc
     1081 agcacgatct cgaaatattt ggccaggccg atgccttcga gcaacggcgt ggtgtaacgc
     1141 tcgaccttgt tggtgatgca gcccagacgg taaccggcgg ccttcattgc ttccagacct
     1201 tcgatcactc catcgaagac ccggctttgc agcaacagat cggtgtagtg cttctcgaaa
     1261 atgggcagcg catgatcgta gagtgcggca tccggcgtgg cgtgcatgtc gccggtcagc
     1321 gcgcgtttca ccagccagct gatgccgttg ccgatgtagc tggccagcag ctcctgcgat
     1381 accggcgcat agtccatctc gcgcagcatg cgattggccg cctcggagag ttcgggcgcg
     1441 gtgtgcagca aggtgccgtc gagatcgatg acgatggctt gcacattaag cggtggagtg
     1501 aagcggatgt tgggttgcat agggtcagtg ttttgccggt ttttcggttg ttgctgtgcc
     1561 agctttggct acagccttga ggtcgggtag cgccatcttt accgtatcgg cattcgtggc
     1621 ggtttcagcg accgcgcttt gtggtgcatg gatgaccgat gctgggcgtt tacccatggt
     1681 cgggtcaatc acgccctttt ctatatcctt gttggcggcc ttccagccgt ccagtaccga
     1741 cgttgggctc tgcgtttcat tctccttcat gcagtcttcc ggcgccttga gcgaaacgcg
     1801 acaggcatag cctatcgctt ccgcatcata gatcttcttg tcggcctcgc ccgagatccg
     1861 gtcgcaaccg gccagcgcga gagccgccag cagcaacagg gatcgcagtg agtgcatgac
     1921 ggggcttaag ccttggccag ttctgcgcgc atcgcggcga ctacgctgtc gtagcgatgc
     1981 ggatcgctat ccttgcctgc gccgaagatg gcggagcccg cgacgaaggt atccacgcca
     2041 gccttggcga cttcgaggat gttggccggg ccgacaccgc cgtcgatttc gaggcggcag
     2101 ttgtagccgc ctgcatcgat catcgcgcgt accttgcgcg ccttgtccag cacatgagga
     2161 atgaacttct ggccgccgaa gccggggttg accgacatca gcaataccat gtcgagttta
     2221 ggcagggtgt attccagaat gtccagcggg gtggatgggt tgaacaccag gcctgccttg
     2281 cagccgagtt ccttgatcag gccgatggtg cggtcgatat gctcggaagc ctccgggtgg
     2341 aaggtgatgt aagtcgcgcc cgccttggcg aagtcgggga tgatgcgatc cacgggcttg
     2401 accatcagat gcacgtcgat cggagccgtc acgccatgct tgcgcagtgc ctcgcatacc
     2461 agtgggccga ttgtcaggtt cggcacgtaa tggttgtcca tcacgtcgaa gtgcacgata
     2521 tccgcgccgg aagcgaggat gttgtcgact tcttcgccca gcttggcaaa gttggcagaa
     2581 agaatgctcg gagcgatttg gaacatggca agacccttca gttagttcga attcaaagtg
     2641 ggcattctaa ccgaaagcgg cgcttcggtt cacctcgggg ttggacggcc gcgctaattt
     2701 tcgctgccgc gtcgcgaccc tgatgaggga agaatgcaaa aaattatgcc gtcggcagcg
     2761 gcaatccggt ggcgcgatag aagttgccca gatcgctcag gtcgttgaac acgccagccg
     2821 cgccgctgaa atcctgattg cgcgtgtagt ggttcagcgt gacgtaggtc ttgattccgg
     2881 cggccagact ggagcgcagg ccgttctcgg agtcttccag cgcgatgcag tcggtggcgg
     2941 gaatgcccag cttgtccagc acccagaaat agatgtccgg cgcgggcttc ttgtgcggca
     3001 cgatgtcgcc gcagccattg gcggcgaaat agtcggccca gtcgcggccg atgccgactt
     3061 ccagcaggcc ggcgacattc tccggcgatg tggtggttga gatggcgagc gcaatgcctg
     3121 cattgcgcgc atcgtctatc agctgccgga cgccggggcg cagcgggatc agtccttcgc
     3181 gcagcatggc gacgtaatgc gcggtcttta ccttgtgcag gtgcttgacg aagtcttcgt
     3241 agtcggcggg cttggcaaaa ccctgcagga agctgtcgac gaaatatttg atgcgctcct
     3301 tgccgccggt cactctcagt agtttgtcat acagcgcgac atcccagttc cagtccaggc
     3361 cgttctcggc gaaggctttg ttgaaagact tgcggtgagc gtcttcggtg tcggccagcg
     3421 tgccgtccac gtcaaaaata atggctttga tcgtcatgat ggattcgtgg tgagttgtaa
     3481 gagtggttaa cgcaggcccg ggaattttcc tgccatgccg cgcatcagtt tgcccatgcc
     3541 gcccttgctg aacattttca tcatcttttg cgactgttcg aactggttga gcagctggtt
     3601 gacgtgcatc acctgcacgc cggcccccat ggcgatgcgg cgtttgcgcg aggccttgat
     3661 gagttcgggt ttgctgcgtt ccagcggggt catcgaattg atgatgccct cgatacggtt
     3721 gatctgtttg tcgtccacct tggcgccggc ggcagcctgg ctgagttgcg cgggcagttt
     3781 atccatcagt gcggacatgc cgcccatgtt gcgcatctgc acgatctgca tcttgaaatc
     3841 gttgaggtcg aagccctcgc ctttctgaac cttcttcgcc agtttctcgg cttcggccat
     3901 gttcacgccg cgctgcgctt cctcgatcag cgacagcacg tcgcccatgc cgagtacgcg
     3961 cgaggccatg cgctccgggt ggaaggcttc gaggccgttg ggtttttcgc tgacgccgac
     4021 aaacttgatc ggcttgccgg tgatctgtcg caccgacagc gccgcgccgc cgcgcgcatc
     4081 gccgtccagt ttggtgagga tcacgccagt caacggcagg gtctcgccga atgcgcgggc
     4141 ggtgttcacc gcatcctgtc cggtcatcgc atccacgacg aacagcgtct cgatcggttt
     4201 gagcgctgcg tgcagttgct gaatctcttc catcatcgcg gtatcgatag ccagccggcc
     4261 tgcggtatcg acgatcagca cgtcgtgata atgacggcgc gcgtagtcca gagcggccag
     4321 cgcgatatcc ttcggtttct ggctgatgtc ggaggcgaag aaatcgattt cgagttgttg
     4381 cgccagtgtg cgcagttgtt cgatggcggc gggacggtat acgtcgcagc tgaccagcag
     4441 caccttcttc ttctgttggt cgcgcagcag cttggccagc ttgccgctgg tggtggtctt
     4501 gcctgcgcct tgcaggcccg ccatcaggat caccgctggc ggggtggtgg cgaggttgag
     4561 cgcgtcgtta tgctcgccca tcagcttggt gagctcgcga tgcaccacgc cgatcagtgc
     4621 ttgccccgga ttcaggttgc cgacgacttc ggcgccgaga gcggcctgtt tgacctgggc
     4681 gatgaagtcc ttgactacgg gcagggcgac gtcggcttcg agcagcgcca tgcgcacttc
     4741 gcgcagagca tcctgaatgt tgctctcgct gagacgcgac tgtccgcgca gattcttgat
     4801 gacgccggag aggcgttgag tcaggttgtc cagcatagtg tcattacgta tggtgtagaa
     4861 tcaccgaagt ctaaaacatc ccgatgaaaa atgtctaacg ttatcctctc ctggttggcc
     4921 tttaccatat atagcgtgct cgcagtctat ttctggcagg cgcaggtgcg gggcaagggg
     4981 gatgcgttga ttcgcggcgc agcagggcac tgggtgttgg tgccgctggc gttgcagggt
     5041 tatttgctgg tgcaggatgt gtttgccgga ggcggattca atctgagcat cgccaacgcc
     5101 ttgtcgctga tcgtctggtt gacgctgggc gtgtattggg tggcgcgctt cttctatccg
     5161 gtcggcggct tgcaggcgct ggtgctgccg ctggcggcgg tggcggtgtt gctgccggag
     5221 atctttccga cctcgcatca gctggagaac accggcctgc tggcattcaa ggcgcacatc
     5281 gctgctgcga tgctggccta cagtttgttt accatcgcca tgctgcatgc ggtgctgatc
     5341 tcgcaggtgg agaagcgcct gcatcatgcg accctgccgc gcgtgctgca gaatctgccg
     5401 ccgctgctga ccatggagac cttgctgttc cgcattatcg gcatcggctt cctgttgctg
     5461 acgctgacgc tggcgtccgg tgtggttttc tccgaagcga tcttcggcaa ggcatggcag
     5521 cttaatcaca aggtgttgtt tggcttcatt tcctggctgg tgtttgccgt gctgttgtgg
     5581 gggcatcgct tccacggctg gcgtggacgc atcgcggtgc gctggacggt gagcggcttt
     5641 gtgttgttgc tgctggccta tttaggcagc aagtttgtca tcgaggtgct tctgcatcgt
     5701 tgagtcccgg aaattccctt gtttttcttg tgcttgccac aagctaggga attgtcgtag
     5761 aattcgcgcc ctttcgctgg gctaataact ttaaataggt tttatatggt aatcattcgt
     5821 ctttctcgtg gtggctccaa gaaccgcccg ttcttcaacg tagtggtggc tgactcgcgc
     5881 aatcgtcgcg acggtcgttt catcgagcgc gttggttttt acaatcctat cgctgcagaa
     5941 ggtcaggaag cactgcgcct gcagatggag cgtgtcagct tctggcagag caagggcgcg
     6001 cagctgagcg acgctgttgc caagctggtt aagcgtgccg gagttgcagc agcttaattt
     6061 aagcgtgcag caacctgacg aagcaggccc catggttatc atggggcgag tggcggccgc
     6121 tcacggtatc cgtggctggg tcaaaataca gccctttacc gagtatcttg acagtctgct
     6181 ggagtacggc acctggtggc tcgggcatga gcaaggcccg tggcgtgaag tcaaggtgca
     6241 gcagagcgaa gtgcatcaca agacgctggc ggctttgctg gagaattgcc ctgaccgtac
     6301 tgcggccgag aagctgaaag gcttgttgat cgcggtgccg cgcagcagtt tgcccgagca
     6361 ggatgaagac gagtattact ggtctgacct gatcggcatg tcggtggtga acgaagcggg
     6421 cgagattctc ggtgtcgtgg aaaatctgct cgaaaccggc gcgaacgacg tgttgagcgt
     6481 caaaggcagc agcggttcga ttctgattcc gttcgtggat tcggcagtca aacaggtgga
     6541 tgtggcaagc aggaccatcc gggtggactg gtcggcggat tatctgtcgt gatattcgac
     6601 gtcatcacgc tgtttccgca gatgttcgag gcgttgacgc agtatggcat cacgcggcgc
     6661 gcggcggagc aaggcaagta tgtgctgaag acgtggaatc cgcgcgactt cactacggac
     6721 aactaccgca ccatcgatga tcgtccctat gggggcggtc ccggtatggt gatgctgggc
     6781 gagccgctgg cgggcgcgat caacgcggcc aagcagcgac aggcagccag cggcgtgaaa
     6841 aagagccggg tggtgtatct ttcgccacag ggccgcttgc ttactcacgg cgtggtcaag
     6901 caattggttg cgcagcagga cgaaggactg attctgctgg cgggacgtta tgaaggcatc
     6961 gacgaacgtc tgatccgcca gtatgtggat gaagagattt ccatcggcga ctatgtgttg
     7021 tccggtggcg agttagcggc gatggtgctg gtggatagtc tggtgcggca actgcccggt
     7081 gtgctgggcg atgccgaatc ggcggaacag gactcgttcg tgcagggcct gctggattgc
     7141 ccgcactaca cccgtcccga agaatttaat ggtgaagccg tgcccccggt gttgttgtct
     7201 ggcaatcatg cggacataca gcgctggcgg cttcagcagt cgttgggcag aacgtggttg
     7261 cgcaggccgg atttattggc caggcgtgat ttgacaaaag aggagtctcg gcttctggct
     7321 cagttccaga aggaacaaga cccgataacc aaggagtagt aaagtgaact tgattgaaca
     7381 gttggaacaa gaagagatcg cccgtctggg caagaccatt cctgtgttcg cccctggcga
     7441 tacagtggtg gtcggtgtga acgtggtcga aggtgagcgc aagcgcgttc aggttttcga
     7501 gggtgtggtt atcgccaagc gtaaccgtgg tctgaactcc gcttttatcg tgcgtaagat
     7561 ttccgcaggt gaaggcgtgg agcgtacgtt ccagacctat tcgcctatca tcgccagcat
     7621 cgaagttaag cgcaaaggcg ccgtgcgtcg tgccaagctg tactatctgc gtgatcgttc
     7681 cggcaagtcg gcgcgtatca aggaaaaact ggcagcacgc ggctaagctg cgagcggttt
     7741 ttctgctacg aaaaaacggg agcctcggct cccgtttttg ttttggccgc catatggtgg
     7801 cttcgcatgc gccgatatca gcctttacgg aggcggtcgc ttgtgcgcta tcatcgcacc
     7861 atggattatc aggcgaaatt aagggtgccg ttcggcgtgc tgggtatacg ctgtagcgca
     7921 gatgcccttg tcggcatcga ttttctcgcc gtgtcggaat ctgcgcagac cccgaccggc
     7981 gaaatcgccg cgatggcttg cgaacagttg cggggctatt tcgatgatcc gcaagcatcg
     8041 ctcaccgtgc cgctcgatct gcgcggcacg gcacatcgac accgggtctg gcaggccata
     8101 ttgcatattc cgtgcggcca gacgcgcagc tacggcgagc tggctgcagc gctgcattcc
     8161 ggcgcgcagg ccgtcggtca ggcctgcggg gtcaatcccg ttcccatcgt cgtgccctgt
     8221 catcgcgtga tcgccaaggc agggctgggc ggattcatgc agagtgctgg gggcgctacg
     8281 ctggacatca agcgctggct gctgaagcat gagggcgtgc tgtgaacaac gccgacctgc
     8341 tcgatgaatt ctgcgacaac ctgtggctgg aagacgggct gtcgcgcaac acgctggaca
     8401 gttatcggcg cgacctgcgc aagttcgggg gctggctgga aaagcagcgc gaggcctctg
     8461 ttctgcaggc tgcgcatggt gacatacagg gttacctcgc gcatctggtg gtcgagcaaa
     8521 aggccaagcc gagttccacc gggcgcaata tctccagtct caagcgtctg tttcgctatc
     8581 tgttgcgtca gggcaagatt gctgtcgatc cgacgctgca gatcgacacc cccaggctgc
     8641 cgcgcaacct gccgaaaagc ctgaccgaac aggatgtcga gctgctgctc gacgcgccgg
     8701 atatgcagac gccattgggg ctgcgcgacc gcacca
//
LOCUS       DASUIV010000244         8932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1085256, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000244 DASUIV010000000
VERSION     DASUIV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085256"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /gene="acnA"
                     /locus_tag="VFW59_08880"
     CDS             <1..1665
                     /gene="acnA"
                     /locus_tag="VFW59_08880"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX5364373.1"
                     /translation="TFNTLFSKPVAENGFNKPAAELGKRYTTALPDRSGAVCVPVSGG
                     GRQDGAQNSSEMEMASDHPTASVRCETTDEMLPPVEIGHGDVLIAAITSCTNTSNPGV
                     LLAAGLLAKKAVAKGLRVKPHIKTTLAPGSRVVTEYLTNAGLIEPLAQLGFTVAAYGC
                     TTCIGNSGPLDAKLEETIVKNDLIAAAVLSGNRNFEARIHANIKANFLASPPLVVAYA
                     IAGKMNINLDTEPLGTGKDGQPVYLKDIWPNSAEVQTVMKYAADPAVYQRLYSDLTKD
                     LPLWNAIAAPTGDRYQWDDSTYIARPPFFDAAAPKVGDIVGAKALALFGDSVTTDHIS
                     PAGSFKPTTPAGQYLQGKHVAVKDFNSYGSRRGNHEVMMRGTFANVRVKNLMLPTKED
                     GSRIEGGYTLYKGEQMAIYDAAMKHIADGTPTVIFAGEEYGTGSSRDWAAKGTQLLGV
                     KAVIAKSYERIHRSNLVGMGVLPLQFKGSDSLASLNLTGDETFDLSGINDNLKPQQDV
                     QLSITRKDGSKQNVALLLRIDTPIEVDYYKNGGILPYVLKELVSKA"
     gene            1902..2525
                     /locus_tag="VFW59_08885"
     CDS             1902..2525
                     /locus_tag="VFW59_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364374.1"
                     /translation="MWFNALRSLRLPFRNVIKRRGFTPIPADAEPRLPMKPSALPHRD
                     DDAVLTLYAHYFLASELMLNNYKKLAKKWQQRGRLTPNDRVQLPIYFATWLGLLAVTA
                     EGFKKLAIHKLVQTDRPPDFVELGSQADELGSLLKKYDDALRRFRNTVFHLREDTQDI
                     ERFFNEQPNRLEWAEELQSAFDKFFSSYRILCQVHYAIENRNDELFK"
     gene            2581..3390
                     /locus_tag="VFW59_08890"
     CDS             2581..3390
                     /locus_tag="VFW59_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364375.1"
                     /translation="MVKPSCDRFIHPVPALPRFSDRSPEVVRTLDRIRFELESDDTLE
                     DDLRLGEFTWFAREYPRCYRHHLECAEFRLRSIHDLVSAIHTELAPSVESNPHMFSCS
                     VGDLRVKRVYWDFESYLCEINNALDLLARIAGLVYRRQTPPNFNRLCKTNEEQTAMLS
                     IMQGAKSHWVTRMKSYRDCFTHFTPVDTMLSVGLEQYADGFHIRAKLPVNPSAREILD
                     FRYSRRVELLRYAITVWRHMSALDRAIAKQIAADYSTGAYPARTTGLFRVS"
     gene            3767..4210
                     /locus_tag="VFW59_08895"
     CDS             3767..4210
                     /locus_tag="VFW59_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364376.1"
                     /translation="MTEERPHPTPPQVNAFRMKEYEVIRAEVMDRLKELWALEKFALG
                     GASAIAAWLFTHFKEVNMKVAWWLPFIFLALCAVRFGTGMYHLTDRAAEHLIRIEKYY
                     MGEKGSWEAWFRKQGRNETWAYLLVWAVALIASFTLPFWVCSSSG"
     gene            complement(4385..5026)
                     /locus_tag="VFW59_08900"
     CDS             complement(4385..5026)
                     /locus_tag="VFW59_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5364377.1"
                     /translation="MSKLRVQSFAISIDGFGAGPDQDLEHPIGVRGMELMEWFFPTRV
                     WRQMQGEEGGETGVDNHIAEQGFANIGAWILGRNMFGPIRGPWPDDSWKGWWGEEPPY
                     HTPVFVLTHHPRAPLRMKGGTEFRFVTEGIHAVLEQAKAAADGLDVRLGGGVSTIRQY
                     LNAGLVDELHLAISPVLLGKGEHLLRDIDMHALGYECEKYVAGERAAHVFLHK"
     gene            complement(5037..6008)
                     /locus_tag="VFW59_08905"
     CDS             complement(5037..6008)
                     /locus_tag="VFW59_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX5364378.1"
                     /translation="MNHAIRIHKYGGPEVLQWEEMAVGDPGPGEIGLKQTAVGLNYID
                     VYHRTGEYPQPLPFVPGMSAAGIVESVGKGVAHLKPGDRVAYPMEIGAYAEARLIAAD
                     RVVKLPDAVTDRMAAAMMLRGMTARYLIRDIYKVGPGDTILIHAAAGGVGLLVCQWAA
                     AMGATVIGTVSSDQKAALAKANGCHHPIVYTREDFQARVMEITDGKKLPVVYDGVGKD
                     TYLKSLDCLRPRGLLVLFGSASGPVPSFDTSLLAQKGSLLLTRPTLGTFIATRAQMEE
                     NAADLFAAVTSGKVQIYINQTWPLKDVAQAHRDLEARKTSGSTVLQP"
     gene            complement(6024..6575)
                     /locus_tag="VFW59_08910"
     CDS             complement(6024..6575)
                     /locus_tag="VFW59_08910"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5364379.1"
                     /translation="MAPSLRKQHFPVEKVRRYLEPGPIVLVSSAWKGKRNIMTMGWHT
                     VMEFSPSLVGCIISSANHSFEMVRRSRECVINVPTTALIDEVVGIGTTTGTEIDKFDH
                     FGLTAGAADSVKAPLIRECHANFECKLADARMVDKYNFFIWEVVSAHVAASPKHPETL
                     HYKGEGTFMVAGRIFHRRKLAQM"
     gene            6789..7484
                     /locus_tag="VFW59_08915"
     CDS             6789..7484
                     /locus_tag="VFW59_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX5364380.1"
                     /translation="MTRDHHIERHFRASQTVRDIVIGMADGLTVPFALAAGLSGAVAS
                     SHIITTAGIAEIAAGSVAMGLGGYLAARGDAEHYATELHRERREVQNVPEEEAAEIVA
                     VFKAYGVTAEQCAPVVEALRANRSAWVRFMMRFELGLERPEPGRAIQSAATIAAAYIV
                     GGLIPIAPYFIATDIGQALPYSVAVTLIALAAFGYAKGRYTGTAALRSALQTLLIGGM
                     AAAAAFLLARAIS"
     gene            7511..8905
                     /gene="fumC"
                     /locus_tag="VFW59_08920"
     CDS             7511..8905
                     /gene="fumC"
                     /locus_tag="VFW59_08920"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5364381.1"
                     /translation="MTSRNESDSFGSIAVPAERLWGAQTQRSLEHFHISTELMPEEII
                     MALVAAKRACALVNRDLGLLDADKAAAVVQAADEVLAGQHPDEFPLSVWQTGSGTQSN
                     MNINEVLANRASELLGGERGAGRLVHPNDDVNLGQSSNDIFPTAMHLAAVIGISQQLL
                     PALRALRAALEGKSEAFAAIVKIGRTHLQDATPLTLGQEFSGYVAQLDLAEALIAAAL
                     APLRELAVGGTAVGTGLNTHAEFGERVAAELTRSTGIAFSSAANKFAALAAHDALIAA
                     HGALKTLAAALMKIANDVRWLASGPRSGLGEISIPENEPGSSIMPGKVNPTQCEALTM
                     LCCQVFGNDVALTVGGASGNFELNVFKPLIAHNFMQSVRLLADGMSSFEEYCVRGITA
                     NHARIAELMERSLMLVTALTPHIGYDRAAGIAKHAHANGSTLKQAALTLGYVTEDEFD
                     RWVQPVHMTHPASK"
BASE COUNT         1916 a         2717 c         2572 g         1727 t
ORIGIN      
        1 actttcaaca cgctgttcag caagcccgtc gcggaaaacg gcttcaacaa accggcagcc
       61 gaactcggca agcgctacac caccgcgctg cccgaccgca gcggcgcagt gtgcgtccct
      121 gtctccggcg gaggccgtca ggacggcgcg caaaacagca gcgagatgga aatggcttcc
      181 gaccacccga ccgcatcggt gcgctgcgaa accacggacg aaatgctgcc gccggtcgag
      241 atcggccacg gcgacgtgct gatcgccgcg atcacatcct gcaccaacac gtccaacccc
      301 ggcgtgctgc tggcagcggg cttgctggcg aagaaggccg tcgctaaagg cctgcgcgtc
      361 aagccgcaca tcaagaccac gcttgcgccc ggctcgcgcg tggtgactga atacctgacc
      421 aacgcaggcc tgatcgagcc gctggcgcaa ctgggcttca cggtcgccgc ttacggctgc
      481 accacctgca tcggaaactc cggcccgctg gatgccaagc tggaagaaac catcgtcaag
      541 aacgacctga tcgccgctgc ggtgctgtcc ggcaaccgca acttcgaggc gcgcatccac
      601 gccaacatca aggccaactt cctcgcctcg ccgccgctgg tcgtcgccta cgccatcgcg
      661 ggcaagatga acatcaacct cgacaccgaa ccgctgggta ccggcaaaga cggacaaccc
      721 gtttacctga aggacatctg gccgaacagc gcggaagtgc agaccgtgat gaagtacgcc
      781 gccgatccgg ccgtgtatca acgcctgtac agcgacctga ccaaagatct gccgctgtgg
      841 aacgccatcg cagctccgac cggcgaccgt taccagtggg acgactccac ctacatcgcg
      901 cgtccgccgt tcttcgatgc tgccgcaccc aaggtcggcg acatcgttgg agcaaaggca
      961 ttggcgctgt tcggcgactc agtcaccacc gaccacatca gcccggcggg cagcttcaag
     1021 ccgaccacgc ccgcaggcca atacctgcaa ggcaagcacg tcgcggtgaa ggacttcaac
     1081 agctacggct cgcgtcgcgg caaccacgaa gtgatgatgc gaggcacctt cgccaacgtg
     1141 cgcgtcaaaa acctgatgct gccgactaaa gaagacggct cgcgcatcga aggcggctac
     1201 acgctgtaca agggcgaaca gatggcgatc tacgatgccg caatgaagca catcgccgac
     1261 ggcacgccga cggtgatctt cgcgggcgag gaatacggca ccggctccag ccgcgactgg
     1321 gcagcgaaag gcacgcagtt gctgggcgta aaggccgtca tcgccaagag ctacgagcgc
     1381 atccaccgca gcaacctggt cggcatgggc gtactgccgt tgcagttcaa aggcagcgac
     1441 tcgctggctt cgctgaatct gaccggcgac gaaaccttcg acctgagcgg catcaacgac
     1501 aacctcaaac cgcaacagga cgttcagctt tccatcacgc gcaaggacgg cagcaagcaa
     1561 aacgtcgcac tgctgctgcg catcgacacg ccgattgaag tggactacta caagaacggc
     1621 gggattctgc cgtatgtgct gaaggagttg gtgagcaagg cgtaagcgtt tagctcacga
     1681 tgcaggaaaa gaggaagccc gcgatgcggg cttctttaat ttgggaagtc taatcctttg
     1741 aaggcggaca gcgcgttctc aatgaacgac agttcttcgg ccatagctgc catcccggcc
     1801 aatgacggta gtggcggctt tgcacctcat tccagcccgc ctccttctta gaggctgagc
     1861 cgaatatcta gatatttcgg tctatatgct caaacaaggt aatgtggttc aatgcgcttc
     1921 ggagtctgcg tcttcctttt cgtaatgtaa taaagagacg cggctttacg ccgattcctg
     1981 ccgatgcaga gccaagattg ccgatgaaac cttcagcact tccccatcgc gacgacgatg
     2041 cagtcctaac gctgtatgca cactacttct tagcaagtga gctgatgctc aacaactaca
     2101 agaagttggc gaagaagtgg caacaacgag gccgcctgac tccgaatgat cgcgtgcaat
     2161 tgccgatcta tttcgctact tggcttggac ttcttgccgt gacggccgag ggcttcaaga
     2221 agctggcgat tcataaactg gttcagactg atcgtccacc ggactttgtc gagttggggt
     2281 cgcaagctga cgagctaggc agccttctga aaaaatacga cgatgctttg aggagattcc
     2341 gaaacactgt cttccatttg cgcgaagaca cgcaagacat agaacgcttt ttcaacgagc
     2401 agccgaaccg cttggaatgg gcagaagagc tccagtcagc attcgataag ttcttttcga
     2461 gctatcgtat cctgtgccaa gtgcactacg cgatcgagaa tcgaaacgat gagttgttca
     2521 agtagaagta catgtggcag gtcggcagta catgaggcct aatgttgatt ggagactccc
     2581 ttggttaagc cttcatgtga tcggttcatt caccctgtac ctgctctgcc gcgattctct
     2641 gaccgctcgc cggaagtagt ccgcactctc gaccgcatca ggttcgaact cgaaagcgat
     2701 gacacccttg aggatgatct gcggctgggg gagtttacct ggttcgctcg tgagtatccc
     2761 cgatgctaca ggcatcatct tgaatgcgcg gaatttcggc tgcgcagcat tcacgatctc
     2821 gtttcggcga tccatactga actcgccccg agtgtcgagt cgaacccgca catgttcagt
     2881 tgttcggttg gagacctgcg agtcaaacga gtgtactggg acttcgagtc gtacctatgt
     2941 gagatcaaca acgctcttga tttgctcgca agaatcgccg gtctcgtcta ccgacgtcag
     3001 actcctccga acttcaaccg actatgcaag accaacgagg aacagacggc gatgctgtcg
     3061 atcatgcagg gtgcgaagtc acattgggta acccggatga agagctaccg cgactgcttc
     3121 acccacttca ctccggtaga cacaatgctc agcgttgggc tggaacagta tgccgacggc
     3181 tttcacattc gggcgaagct gcccgtgaac ccttcggccc gggagattct cgactttcgg
     3241 tactctcgac gagtggagtt gctccgatac gccatcacgg tgtggcgaca catgtcagcg
     3301 ctggatcggg ccatcgctaa gcagatcgct gctgactaca gcacgggcgc gtatccggcg
     3361 cgtacaacag gattattcag agtgtcatag caacaatcta acaatggcat cgagcggacg
     3421 gtgccgtcgc tcatttcaac cccgttgcac ggctggttac tagcgcaaaa cggaagtccc
     3481 acggcattgt ttgcaacgac agctaaggcc gaaaagtgga aggtcgcagc caacgtaggg
     3541 cgcaataacc aatgggcatt gcgccgtatg atcgcaaaca tccggtgcaa tgcgctacgc
     3601 ttattgcacc ctacatcact acataacctc ctctcccaat atagataatt gtgcatatat
     3661 gcctgaacgt aaaaaccctg gctcaatgca ttttaactat aacgctatgc gggtttgaac
     3721 gaattgcgga ggcatcgata aatcaatcat ccgcctagga gaagccatga ccgaagaaag
     3781 accgcatccc accccaccgc aagtcaacgc cttcaggatg aaggaatacg aagtcattcg
     3841 ggccgaggtg atggatcgct tgaaggaact ctgggcactg gagaagtttg ctcttggtgg
     3901 tgcatccgca attgcagctt ggctattcac acacttcaaa gaagtaaaca tgaaggtcgc
     3961 ctggtggctt cctttcatat ttctcgcgct ttgtgctgtt cgctttggga cgggcatgta
     4021 ccatttaaca gatcgcgcag cagaacatct gattcgcatt gaaaaatact acatgggcga
     4081 gaaaggtagc tgggaggctt ggtttcgcaa gcaagggcga aatgaaactt gggcgtatct
     4141 tttagtatgg gctgtcgcgt tgatcgcatc cttcacgctg cctttttggg tgtgctcaag
     4201 ttcaggttaa ctcacctagc agcaggaagc gataaaacag cgcctagact aagggggtaa
     4261 gaaattttgt gtaaacggtc ataaggtagg ccaccccaaa tacagcgccc aaaagttgct
     4321 cagttagcgc ggatgaaatc cagcacggca gtcaggactt catcatcccg ccacatgatc
     4381 aggctcactt gtgcaaaaat acatgagccg cgcgttcacc ggcgacgtat ttctcgcatt
     4441 cgtagcccaa ggcatgcatg tcgatgtcgc gcagcaggtg ttccccttta ccgagcagga
     4501 cggggctgat ggcaaggtgc agttcgtcga ccagtccggc gttcaggtac tgccgtatgg
     4561 tagacacgcc gccgccaagc cggacatcga gaccgtctgc ggcggctttc gcctgttcca
     4621 gcacagcgtg tatgccttcg gtgacaaagc ggaactcggt gccacccttc atcctgagcg
     4681 gcgcgcgcgg atggtgcgtc agcacgaaga ccggcgtgtg gtacggcggc tcttcgcccc
     4741 accagccttt ccagctatcg tccggccacg gaccgcggat ggggccgaac atgttgcgtc
     4801 ccagtatcca cgcaccgatg ttggcgaagc cctgttccgc gatgtggttg tcaacgccgg
     4861 tctcgccgcc ctcctcgccc tgcatctgcc gccacactcg agtggggaag aaccattcca
     4921 tcaactccat gccgcgcacg ccgatggggt gctcaaggtc ctggtcaggc ccggcaccga
     4981 aaccgtcgat ggagatagcg aaactctgca cacggagctt ggacatggtt atctcctcag
     5041 ggctgcaaca ctgtcgagcc gctcgtcttg cgcgcttcga ggtcacggtg cgcctgcgcc
     5101 acgtctttca gcggccaggt ctggttgatg taaatctgta ccttgcccga ggtcaccgca
     5161 gcgaacaggt ctgccgcgtt ctcttccatc tgtgcccgcg tggcgatgaa ggtgcccaag
     5221 gtcggccggg taagcagcag ggagcccttc tgcgcgagca gcgacgtgtc gaaggatggg
     5281 accggccccg aggcggagcc gaacagcacc agcaggccgc gcggacgcag gcagtcgagc
     5341 gacttcaggt aagtgtcctt gccgactcca tcgtagacca cgggcagctt cttgccgtcc
     5401 gtgatctcca tcacgcgcgc ctgaaaatcc tctcgggtat agacgatggg atggtggcag
     5461 ccattggcct tggccagcgc cgccttctgg tcactggaca cggtgccgat cacggtcgcc
     5521 cccatcgctg ccgcccactg gcacaccagc aggccgacgc cgccagctgc ggcatggatg
     5581 aggatggtgt cgcccggccc gaccttgtag atgtcgcgga taaggtaacg cgcggtcatg
     5641 ccgcgcagca tcatcgcggc cgccatgcgg tcggtgacgg cgtccggcag tttcaccacg
     5701 cggtcggccg cgatcaggcg ggcctcggca taggcgccga tctccatggg ataggccacc
     5761 cggtcgcccg gcttgagatg cgcgacgccc ttgcctaccg actcgacgat accggccgca
     5821 ctcatgccgg gtacgaaagg cagcggctgc ggatactcac cggtacggtg atagacatcg
     5881 atgtaattca gacctaccgc cgtctgcttc agcccgatct cgcccggccc cggatcgccg
     5941 actgccatct cttcccattg caacacttcg ggaccaccgt acttgtggat gcggattgcg
     6001 tggttcatgt tcgtctcctt tgttcacatt tgcgccagct tgcgccggtg gaatatcctg
     6061 cccgcgacca tgaaggtgcc ctcgcccttg taatgcagcg tctccggatg tttgggcgat
     6121 gccgccacgt gcgcgctcac gacttcccag atgaagaagt tgtacttgtc gaccatgcgg
     6181 gcatcggcca gcttgcattc gaagttggca tggcattcac ggatcagcgg cgccttgacg
     6241 ctgtccgccg cgcctgcggt gagcccgaaa tggtcgaact tgtctatctc ggtgccggtg
     6301 gtggtgccga tgccgacgac ctcgtcgatc agtgccgtcg tcggaacatt gatcacgcac
     6361 tcgcggctgc ggcgcaccat ttcgaaactg tggttggcgg aagagatgat gcagccgacc
     6421 agcgacggcg agaattccat cacggtatgc cagcccatgg tcatgatgtt gcgcttgccc
     6481 ttccacgccg acgagaccag tacgatgggc ccgggttcga gatagcgtcg caccttttcc
     6541 acgggaaaat gctgcttgcg caaagagggt gccatgcgcg actccttgtc acttgatcat
     6601 gctgcaaact agcgccgcta ccgctggcag gcaatacgct aaatggagta atacaactaa
     6661 agtctattgc ccttccctca ctgatccaag gatcacatcc ttgatcgtcc cggaagaatt
     6721 tcaaccttcg atatccccat ccattacaat gcattccact tgtcgcccta taagccgagc
     6781 cacaccccat gacccgagac caccatatcg aacgacactt ccgcgcctcg cagacggtgc
     6841 gcgacatcgt catcggcatg gcggacgggc tgactgttcc gtttgcactg gcggcgggct
     6901 tgtccggcgc ggtggcttcc agccatatca tcaccacggc gggcatcgcg gaaatcgcgg
     6961 cgggttcggt ggcgatgggc ttgggggggt acctcgcggc gcgtggcgat gccgagcatt
     7021 acgcgacgga attgcaccgc gaacggcgag aggtacaaaa cgttcccgag gaagaagccg
     7081 ccgaaatcgt cgcggtgttc aaggcctacg gggtaactgc ggaacagtgc gcgccggtgg
     7141 tcgaggcatt gcgcgccaac cggagcgcct gggtgcgatt catgatgcgc ttcgaactcg
     7201 gtctggaacg tcccgagccg ggacgcgcca tacaaagcgc ggcgaccatc gcggcggcct
     7261 atatcgtcgg cgggctgatt cccattgcgc cctatttcat cgcgaccgac atcggtcagg
     7321 cgctccccta ctccgtcgcc gtgacgctga tcgcattggc ggcattcggt tacgccaagg
     7381 gccgctacac gggcaccgca gcattgcgca gcgcgctgca aacgctgctg atcggcggca
     7441 tggctgcggc agccgcattt ttattggccc gagcaatttc atagtttcaa tttcatcgcg
     7501 gagtgacgtc atgaccagca gaaatgaaag cgactcattc ggcagcatcg cggtacctgc
     7561 cgaacggctg tggggtgcgc agacgcagcg ctcgctcgaa cacttccaca tttccaccga
     7621 gctgatgccg gaagaaatca tcatggcgct tgttgcggca aaacgcgcct gcgcgctggt
     7681 caatcgcgat ctcggcctgc tggatgccga caaggcggca gccgtcgtgc aggccgccga
     7741 tgaagtgctg gccggacagc atccggatga atttccgctc tcggtgtggc agacaggctc
     7801 gggcacacag agcaatatga acatcaacga ggtgctcgca aaccgcgcct cggaactgct
     7861 cggcggcgaa cgcggcgcgg gccggttggt gcatcccaat gacgacgtta atctcggtca
     7921 gtcatccaac gacatcttcc ccaccgcgat gcatctggcc gccgtcatcg gcatttcaca
     7981 acaactcctg cccgcattac gcgcattgcg cgcagccctg gaaggtaaat cagaagcgtt
     8041 cgccgccatc gtcaaaatcg gccgcaccca tttgcaggac gccactccgc tgacgctggg
     8101 gcaggagttt tcaggctatg tcgcgcaact ggatctggcg gaggcactga tcgccgccgc
     8161 gctggctcca ctccgcgaac tggcggtcgg cggtacggca gtgggaaccg gactgaatac
     8221 ccatgccgaa ttcggcgagc gcgtcgccgc agagttgacg cgcagcacag gcatcgcatt
     8281 cagcagcgca gccaacaaat ttgccgcgct ggccgcccat gacgccctga tagccgcaca
     8341 cggcgcgctc aagacgctag ccgcagcgct gatgaaaatt gccaacgacg tgcgctggct
     8401 ggcctccggc ccgcgttcag gtttaggcga aatcagcatc ccggaaaacg agccgggcag
     8461 ctcgatcatg ccgggcaagg tgaacccgac ccagtgcgaa gcgctgacga tgctgtgctg
     8521 ccaggtgttc ggcaacgatg ttgcgctcac cgtcggcggc gcttcgggca acttcgaatt
     8581 gaatgtgttc aagccgctca tcgcgcacaa cttcatgcag agcgtgcggc tgctggccga
     8641 cggcatgagc agcttcgagg aatattgcgt gcgcggcata acagccaatc atgcccgcat
     8701 cgcggaactg atggaacgtt cgctgatgct ggtgaccgcc ctgaccccgc atatcggcta
     8761 cgaccgcgct gccggaatcg ccaaacatgc gcatgccaac ggcagcacgc tgaaacaggc
     8821 ggcgctaacg ctgggttatg tcacggaaga tgaattcgac cgctgggtgc agccagtaca
     8881 catgacccac ccggcatcga aatagcgaga atcagtacgc actgccggag tc
//
LOCUS       DASUIV010000245         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_434452, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000245 DASUIV010000000
VERSION     DASUIV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434452"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /gene="glmS"
                     /locus_tag="VFW59_08925"
     CDS             <1..962
                     /gene="glmS"
                     /locus_tag="VFW59_08925"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5364382.1"
                     /translation="KVNAVTFLACGTSYHAAKVATYWLEEIAGIPAVAEIASEYRYRT
                     SVPSPTALVIVVSQSGETADTLAALNHAKMLGQDKTLTICNVPESALTRASRLKFLTR
                     AGPEIGVASTKAFTTQLAALFLLTLVLAKQRGRLTQQQESQYLHELRHLPSAVQKVLD
                     LEPQIAKLAERFADKHNALFLGRGMHFPIALEGALKLKEISYIHAEAYPAGELKHGPL
                     ALVDKDMPVISVAPNDALLEKLKSNLQEVRARGGELYVFADADTQIRTADGIHVMQMP
                     EHAGYLSPILHTIPLQLLAYYVALQKGTDVDKPRNLAKSVTVE"
     gene            1138..1902
                     /locus_tag="VFW59_08930"
     CDS             1138..1902
                     /locus_tag="VFW59_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5364383.1"
                     /translation="MAVIAVFNQKGGVGKTTTCLNVTAALNLARQSPIALDMDPQGHL
                     TLASGAARTTADAGMAGFFKHKTALPELFQKTPRGWNLIPASLELAKIDAMFGSDPKA
                     ATLLRRGMQEEIAFVGSPVLIDCCPMLGVLTLNALLACDRVLIPVSADFLSLQGVHRL
                     DSALNVLEKKLNRRFDRKIVVTRFDSRRKLAFEVDDKLRERYGALVCTTRIGENAALA
                     ASPMHGQDVFEYASFSPGAADYRTLTEELWEGGFFV"
     gene            complement(1957..3477)
                     /gene="ubiB"
                     /locus_tag="VFW59_08935"
     CDS             complement(1957..3477)
                     /gene="ubiB"
                     /locus_tag="VFW59_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEX5364384.1"
                     /translation="MRLLRLLKIVFVVLRFGLDEFLLAHARLRWMRPPLNMLLFMRDT
                     SQPRAVRLRLALEALGPIFVKFGQMLSTRRDLIPADLADELARLQDQVPPFPSAQAIA
                     QLEAAYGRPLSQVFKSFDETPVASASVAQVHFAVLPDGRDAAVKILRPGIARVIEHDI
                     ALLDICADLMERLWEDGRRLKPRKVIEEFEKYLRDELDLMREASSASQLKRNFANSPL
                     LIVPEVYWDFCSTEVMVMQRMYGMPISQVGALREAGIDIPRLAANGVEIFYSQVFRDG
                     FFHADMHPGNVQVAADGRYIALDFGIMGTLTDSDKHYLAQNFIAFFRRDYKRVAEVHI
                     ESGWAPADTRVDELEGAIRAVCEPIFDKPLREVSFGRVLLRLFQTSRRFGIEVQPQLV
                     LLQKTLLNIEGLGLQLDPELDLWKTAKPWLESWMGEQVGWRGFLKSLREEAPHYATLL
                     PQLPRLLHRHLEKAQTHPQDEILRREFERLSRRNNALSILVLLLLALLVWQQAAVS"
     gene            complement(3482..>3805)
                     /locus_tag="VFW59_08940"
     CDS             complement(3482..>3805)
                     /locus_tag="VFW59_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364385.1"
                     /translation="SEGDAALLTEIFFLSRNLHWDAAEDLSHVTGDVAAERIVQAAQD
                     TKQHLFAAATSLSQAAAEYWTEERPLLVKPGQIATFVGQVDALRDDIARLEQRIKRLQ
                     SSEYP"
BASE COUNT          792 a         1184 c         1151 g          678 t
ORIGIN      
        1 gcaaggtgaa cgcggtgacg ttcctcgcct gcggcaccag ctaccatgca gcgaaggtgg
       61 ccacgtactg gctggaggag atcgccggca ttcctgcggt tgccgagatc gccagcgaat
      121 accgctatcg caccagcgtc cccagcccga cggcgctggt gatcgtcgtc tcgcagtccg
      181 gcgagactgc cgacaccctg gcggcgctca accacgccaa gatgctgggc caggacaaga
      241 cactgacgat ctgcaacgtg ccggagtcgg cgctcacccg ggcctcgcgc ctcaagttcc
      301 tcacccgcgc gggaccggag atcggcgtcg cctcgaccaa ggcgttcacc acccagctcg
      361 ccgcgctgtt cctgttgacc ctggtgctgg ccaagcagcg cggccgcctg acgcagcagc
      421 aggagagcca atacctgcac gagctgcgcc acctgcccag cgcggtgcag aaggtgctgg
      481 atctggagcc gcagatcgcc aagctggccg agcgtttcgc cgacaagcac aacgcgctgt
      541 ttctcgggcg cggcatgcat ttcccgatcg cgctggaagg cgcactcaag ctcaaggaga
      601 tctcttacat ccacgccgag gcttatccgg caggcgagtt gaagcacggc ccgctggcac
      661 tggtggataa agacatgccg gtgatttcgg tcgcaccgaa cgatgcgctg ctggaaaaac
      721 tgaaatccaa cctgcaggaa gtgcgcgccc gcggcggaga actgtacgta ttcgccgatg
      781 ccgacaccca aatccggacg gcggatggca tccacgtcat gcaaatgccc gaacacgcag
      841 ggtatttgag ccctattctg cacaccatcc cgctgcaact gctggcatat tacgtcgcac
      901 tgcaaaaggg aacggatgtg gacaagccga gaaacctcgc aaagtcggta acagtagaat
      961 agctgggagg cgatgacgca aggtttctcg gcttgcggtg aagactctcg tacccggagg
     1021 gtgaacttgc aaagcaagtt aagcccgaaa gggcggtcgg agccacaagc cccgcatttt
     1081 tgctaagcca taataatggc gaggacgatt ttttcgtctg atattcagga gtaccacatg
     1141 gcggtaatcg ccgtattcaa tcaaaaagga ggggtgggta aaaccactac ctgcctgaac
     1201 gtgactgccg cgctgaatct ggcacggcag tcgcccatcg ctctggatat ggatccgcag
     1261 ggacacctga cgctggccag cggcgccgca agaaccactg cggacgccgg catggcgggc
     1321 tttttcaaac acaagaccgc cttgccggag ctctttcaaa agacgccacg cggctggaat
     1381 ctcatcccgg catcgctgga actggccaag atcgatgcga tgttcggcag cgaccccaag
     1441 gcggcgacgc tgttgcggcg cggcatgcag gaagaaattg ctttcgtcgg ctcgcccgtg
     1501 ctgatcgatt gctgtccgat gctcggggtg ctgacactca atgcgctgct ggcatgcgac
     1561 cgcgtgctga tccccgtatc ggccgacttc ctttccctgc agggagtgca tcgtctggac
     1621 agcgcgctga acgtgctgga gaagaagctc aaccgccgct tcgatcgcaa gatcgtcgtg
     1681 acccgcttcg actcgcgccg caagctggca ttcgaagtcg atgacaaatt gcgcgaacgc
     1741 tacggcgccc tggtctgcac cacgcgcatc ggcgagaatg ccgcactggc agccagcccg
     1801 atgcatggtc aggacgtatt cgaatacgca tccttcagcc cgggtgcggc agattaccgg
     1861 accctgaccg aggaattatg ggaaggcggg ttcttcgtct gaccctccca gcaaaaagcc
     1921 ccgtcatgac ggggcttttt gtttgacctg acaaggctag gagactgcgg cttgttgcca
     1981 gaccagcaag gcgagcagca acaggaccag tatcgacagc gcgttattac gccggctcag
     2041 ccgttcaaat tccctgcgca agatttcgtc ctgagggtgg gtttgagcct tctcgagatg
     2101 gcgatgcagc agacggggca gttgcggcag cagggtggcg taatgcggcg cttcctcacg
     2161 caaggatttc aggaagccgc gccagccgac ctgctcgccc atccagcttt ccagccaggg
     2221 cttggcggtc ttccacagat ccagctccgg atcgagttgc aatcccagtc cttcgatgtt
     2281 gagcagcgtc ttctgcagca gcaccagttg cggctggacc tcgatgccga aacggcgtga
     2341 tgtctggaac aggcgcaaca gcacgcgccc gaacgacacc tcgcgcagcg gcttgtcgaa
     2401 gatcggctcg catactgcgc ggatcgcacc ctcaagttcg tccactcggg tatcggctgg
     2461 cgcccagccg gattcgatat gcacctcggc gacacgcttg taatcgcggc ggaagaaggc
     2521 gatgaagttc tgcgccagat aatgcttgtc gctgtcggtc agcgtgccca tgatgccgaa
     2581 atccagcgcg atatagcggc cgtcggcggc cacctgcacg ttgcccggat gcatatcggc
     2641 atggaagaag ccgtcgcgga acacctgcga atagaagatc tccaccccgt tcgctgccaa
     2701 acggggaatg tcgataccgg cctcgcgcaa cgcacccacc tggctgatcg gcatgccgta
     2761 catgcgctgc atcaccatca cctcggtgct gcaaaaatcc caatacacct caggcacgat
     2821 caacagcggc gaattggcga agttgcgctt gagctggctg gcgctggaag cctcgcgcat
     2881 cagatcgagt tcgtcgcgca gatatttctc gaactcctcg atcaccttgc gcggcttgag
     2941 acggcggcca tcttcccaca gccgctccat caggtcggcg cagatgtcga gcagcgcaat
     3001 gtcatgctcg atcacgcgcg cgataccggg acgcagaatc ttgaccgcag catcgcgccc
     3061 gtccggcagc acggcaaaat gcacctgtgc caccgatgcg ctggcaaccg gcgtttcgtc
     3121 gaagctcttg aacacctgac tcagcggcct gccgtaagcg gcttccagct gggcgatggc
     3181 ctgggcggaa ggaaagggcg gaacctgatc ctgcaaccgg gccagttcgt cggccaaatc
     3241 ggcgggtatc aggtcgcggc gcgtcgataa catctggccg aatttgacga agatcggacc
     3301 gagcgcttcc agcgcgagtc gcaaccgtac tgcgcggggt tgcgaggtat cgcgcatgaa
     3361 cagcagcatg ttcagcggag gccgcatcca gcgcagacgg gcgtgggcga gcagaaattc
     3421 atccagcccg aaacgcagga cgacaaatac gattttcaac agacgcaaca ggcgcagcat
     3481 gctaggggta ctcggaagac tgcagacgtt tgattcgttg ttccagacgc gcgatgtcgt
     3541 cgcgcagcgc atcgacctgc ccaacgaagg tcgcgatctg cccgggcttg accagcagcg
     3601 gacgctcctc ggtccagtat tcggcggcag cctgtgacag actggttgcc gctgcaaaca
     3661 gatgctgttt cgtatcctgc gccgcctgca cgatacgctc ggccgcgaca tcaccggtga
     3721 cgtggctcaa gtcttcggcc gcatcccagt gcaggttgcg cgacaggaag aagatttccg
     3781 tgagcaaggc ggcatcgccc tcgct
//
LOCUS       DASUIV010000246         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1179219, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000246 DASUIV010000000
VERSION     DASUIV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179219"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /gene="glyS"
                     /locus_tag="VFW59_08945"
     CDS             <1..1636
                     /gene="glyS"
                     /locus_tag="VFW59_08945"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX5364386.1"
                     /translation="DGWSSVHFVRPAHGLVALHGADIVAISVLGLEAGRNTQGHRFEA
                     ANAVVTLKDADSYARQLETEGAVVASFAARRAEIVRQLTSAAAKEGLKPIDDDALLDE
                     VTALVERPNVLLGQFETEYLEVPQECLILTMKANQKYFPLLDANGKLTNKFLIVSNIR
                     PADASLVIGGNERVVRPRLADAKFFFDQDRKRTLASRVEGLTKVVYHNKLGTQGERVE
                     RVWAIAQAIALQLGGDAMLAAQAGEAAMVAKADLLTDMVGEFPELQGIMGRYYAQHDG
                     LSDDIAYAIEDHYKPRFSGDELPRNTVGICVALADKLETLAGMFGIGQIPTGDKDPFA
                     LRRHALGVIRMLVEKEMPLSLFSLVNIAFAGFSKGLLTNAHAEVETFIYERLGGVMRE
                     QGYSTNEVEAVLCMKPLQLYLVPKQLEAVRAFAALPEAAALAAANKRVGNILKKVEGE
                     VGADVAAALLQEPAEQALHQALGEIAPKADAAFVAGDYTASLQSLAALREPVDAFFDN
                     VMVNAEDEKLRANRLALLARLQQAMNRVADISKLAS"
     gene            1730..>2259
                     /gene="gmhB"
                     /locus_tag="VFW59_08950"
     CDS             1730..>2259
                     /gene="gmhB"
                     /locus_tag="VFW59_08950"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX5364387.1"
                     /translation="MNLVILDRDGVINYDSDQFIKNPEEWKPLPGSLEAIARLNQADY
                     RVVVATNQSGVGRGLFDMAMLNAIHDKMHKACALVGARIDAVFYCPHTNDANCQCRKP
                     KSGMLEEIAARYGVSLAGVPVVGDSLRDLEMAERMEAQPILVLTGKGTKTQAKGGLPA
                     GTLIYPDLAAVAATLV"
BASE COUNT          452 a          644 c          748 g          415 t
ORIGIN      
        1 cgatggctgg agcagtgtgc acttcgtgcg ccccgcacat ggtctggttg cgttgcacgg
       61 tgcggacatc gtggcgatct ccgtgctggg actcgaggcc gggcgcaata cgcagggcca
      121 tcgcttcgag gctgcgaatg cagtcgtgac gttgaaggat gccgacagct atgcacggca
      181 actggaaacg gaaggtgcgg tcgtcgccag ctttgccgca cgccgtgcgg agatcgtcag
      241 gcaattgacc tcagccgcag cgaaggaagg cctcaagcct atcgatgacg atgctctgct
      301 ggacgaagtg accgcgctgg tcgagcgccc caatgtgctg ctcggccagt tcgagaccga
      361 gtatctggaa gtgccgcagg aatgcctgat cctgacgatg aaggcgaacc agaaatattt
      421 cccgctgctg gatgcgaacg gcaaactgac caataaattc ctgatcgtgt cgaacatccg
      481 ccctgcggat gcgagtctgg tgatcggcgg caacgagcgc gtggtgcgtc cgcgtctggc
      541 cgatgcgaag ttcttcttcg atcaggatcg caagaggacg ctggcctcgc gcgtggaagg
      601 tctgaccaag gtggtgtacc acaacaagct gggtacgcag ggcgagcgcg tggagcgcgt
      661 gtgggcgatc gcgcaggcga tcgcgttgca gctgggtggc gatgccatgc tggctgcgca
      721 ggcgggcgag gcggcgatgg tggccaaggc cgatctgctc accgacatgg tcggcgagtt
      781 ccccgagctg caaggcatca tggggcgtta ttacgcgcag catgacggcc tgtccgacga
      841 tatcgcctac gcgatcgaag accattacaa gccgcgtttc tccggcgacg aattgccgcg
      901 caataccgtc ggcatctgtg tcgcgctggc ggacaagctg gaaacgctgg cgggcatgtt
      961 cggcatcgga cagatcccga ccggcgacaa ggatccgttc gcgctgcgcc gtcacgcgct
     1021 gggtgtgata cgcatgctgg ttgaaaagga aatgccgttg agcctgttca gtctggtcaa
     1081 catcgccttc gccggattct ccaaaggatt gctgaccaac gcccatgccg aggtggagac
     1141 cttcatctac gagcgtctgg gcggtgtgat gcgcgagcag ggctatagca ccaacgaggt
     1201 cgaggcggtg ttatgcatga aaccgctgca gctttatctg gtgccgaaac agctggaagc
     1261 ggtgcgcgcc ttcgccgcat tgccggaagc cgccgcattg gccgcagcca acaagcgcgt
     1321 cggcaatatt ctgaagaagg tcgagggcga ggtgggcgcc gacgtcgccg cagcgttgtt
     1381 gcaggagcct gccgagcagg cgttgcatca ggcgctgggc gagatcgccc ccaaggccga
     1441 tgccgccttc gttgcgggcg actacaccgc ttcgctgcaa tctttggccg cgttgcgcga
     1501 acctgtggat gccttcttcg ataacgtgat ggtgaacgcc gaggatgaga agctgcgggc
     1561 caatcgtctc gcgctgctgg ctcggttgca gcaggcgatg aaccgcgtcg cggacatctc
     1621 caagctggcc tcttaatgac aacaggaagt ggggagtggt aagtaaaacc gcgcaacgac
     1681 ttccgactta cgacttacca cttaccactc acaactgacc attacaaaca tgaacctcgt
     1741 tatcctcgac cgcgacggtg tgatcaatta cgactccgac cagttcatca agaacccgga
     1801 ggaatggaag ccgctccccg gcagcctgga agcgatcgcg cggctgaatc aggcagacta
     1861 tcgcgtggtg gtggcgacca accagtccgg cgtcgggcgc gggctgttcg atatggcgat
     1921 gctgaatgcg atccacgaca agatgcacaa ggcctgcgcg ctggtcggcg cgcgtatcga
     1981 tgcggtgttc tattgccctc ataccaatga tgcgaactgc cagtgccgca agcccaagag
     2041 cggcatgctg gaggagatcg cggcgcgtta cggcgtgagt ctcgccggag tgccggtggt
     2101 gggcgattcc ctgcgcgacc tggaaatggc agagcgcatg gaggcacaac caattctggt
     2161 gctcaccgga aaaggcacga aaacacaggc caagggcggt ttgcctgcgg gcacattaat
     2221 ctatcccgac ctggcggcgg tggcggcgac tctggtatg
//
LOCUS       DASUIV010000247         3646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_61973, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000247 DASUIV010000000
VERSION     DASUIV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61973"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFW59_08955"
     CDS             <1..253
                     /locus_tag="VFW59_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5364388.1"
                     /translation="GLKALGGNRYEARGKLSIKGRTLDIVAPVTFQATGNRGSFDGSF
                     TIKRLDYAIGEGEWTDVGTVANEIQVKFHLVVNATPSKK"
     gene            287..853
                     /locus_tag="VFW59_08960"
     CDS             287..853
                     /locus_tag="VFW59_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5364389.1"
                     /translation="MKKIVALTLASLLSSAAYAAPETYVIDPNHSMPRFEYSHFGYSV
                     QLSRFDSTSGKITIDRAAKTGSVDVTIDAKSVNTGSSLFNGHIQGEDFFDTAKYPTIT
                     FKSSKVKFDGDKVASVDGVLTVKGITKPVTLTLNSFLCMPHPMMKKDACGATATTKVL
                     RSDFNMGKYAPYVGDEVTLTIPVESIKQ"
     gene            949..2016
                     /locus_tag="VFW59_08965"
     CDS             949..2016
                     /locus_tag="VFW59_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX5364390.1"
                     /translation="MTQTLFTPTTLGKLQLKNRIVVAPMTRCRATDNVPNELMEKYYR
                     LRAEAGLIIAEGTSPSANGLGYARQPGLFTAAQIQGWKRVNDGVHAAGGKIFVQLMHD
                     GRVSHPANMLAGAKVLAPSAIAAPGEMWTDSKGMQPYPVPAEMTEADIKQAIDEFATA
                     AKNAIEAGFDGVELHGANGYLIDQFLNTATNQRTDRWGGSVENRIRFAVEIAKAAAAA
                     IGADRIGMRISPYGAFNGAAPDAEMDAMYHRLISELNAIGLVYIHIVDHSAMGAPEVS
                     PTLKAWIRSNFKGKYILSGGYDLARANADLDAQRGDLVAFGRPFISNPDLVQKMKSGT
                     ALTEPDFNTFYTPGEKGYTDY"
     gene            complement(2100..2960)
                     /locus_tag="VFW59_08970"
     CDS             complement(2100..2960)
                     /locus_tag="VFW59_08970"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-class carbonic anhydrase"
                     /protein_id="HEX5364391.1"
                     /translation="MFKKQFMVALAATVFSAAVYADVHGHDAMHAEHGTEGPACTGFG
                     PQTPRDIDNKHGENKIGFSLAPDYKEMNLCNIHFHVNAEHKAKEFSIHAEGEHGADGG
                     YQCNMSKHLSAAELAPTAEPVCPSEHGDLKPGDTIEVHWVHSSAPVTPGPTLGACLTD
                     NVKNPDLRVESQVFTLVNDPKALDFNTLDYHGKVVNGYHQADGIPGNTGKPVLFAGST
                     TGPKYNDQTCSPLQVTWSVRPSCAKLDINSVAKWCKGNAFKEDHAHGVRKLVTEPSLL
                     SEIKGDKSKH"
     gene            complement(3056..>3646)
                     /locus_tag="VFW59_08975"
     CDS             complement(3056..>3646)
                     /locus_tag="VFW59_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX5364392.1"
                     /translation="IDQTEAMTTVDVNTGGFVGLRNFDDTIFKTNLEATQVIARQLRL
                     RNLGGIIICDFIDMDTQEHRDAVLDEFKKALARDHTRIGVNGFSSLGLVEMTRKRTRE
                     SLAHVLCQPCPTCQGRGEVKTAQTVCYEILREILREARQFNAREYRILASQQVVDLFL
                     DEESQALAQLSDFIGKPISMQVETLYGQEQYDVILM"
BASE COUNT          792 a         1110 c          995 g          749 t
ORIGIN      
        1 cggattgaaa gccttgggcg gcaaccgtta tgaggctaga ggcaaactca gcatcaaggg
       61 ccggacactg gatatagttg cacccgtcac ttttcaggcc acaggcaatc gcggttcatt
      121 cgacggcagc ttcaccatca agcggctcga ctacgccatc ggcgaaggcg aatggaccga
      181 tgtcggcacc gttgcaaatg aaattcaagt caagtttcac ctcgtcgtta atgccacacc
      241 gagcaagaag tagttttaca tccaaccacc acctaaggag aacaccatga aaaaaatagt
      301 tgccctgacg ctcgcatccc ttctgtcttc cgctgcttat gccgcaccgg aaacttatgt
      361 gatcgacccg aatcacagca tgccgcgctt cgagtacagc cacttcggct actccgtaca
      421 actgagccga tttgactcga cttccggcaa gatcacgatc gatcgcgccg ccaagacagg
      481 ctcggtcgat gtgaccatcg acgccaaatc ggtcaacacc ggctcttctt tgttcaacgg
      541 ccatattcag ggcgaagact tcttcgacac cgcaaaatat cccaccatca ctttcaagtc
      601 gagcaaggtg aagttcgacg gcgacaaagt ggcttccgtg gatggcgtgc tgacggtcaa
      661 gggcatcacc aagcccgtga cgctgacgct gaattccttc ctgtgcatgc cgcacccgat
      721 gatgaagaaa gacgcctgcg gcgcgacagc cacgaccaag gttttgcgtt ccgatttcaa
      781 tatgggcaag tacgcgcctt atgtcggcga cgaagtgacg ctgactatcc ctgttgagtc
      841 gatcaaacaa taaaacccca cccctttgtc cgcagatgaa cgcagatttt ttgttttaat
      901 ctgtgtaatc tgcggatcgt ttttgatttc gattttaagg agtaccacat gacccaaacc
      961 ctgttcaccc cgaccacgct cggcaaactg caattgaaga accgcatcgt cgtggccccg
     1021 atgacccgct gccgggccac agacaatgtt ccaaacgagc tgatggaaaa atattaccgg
     1081 ctacgcgcag aggcgggact gatcatcgcg gaaggaacct cgccatcggc aaacggcctg
     1141 ggctacgccc gtcaacccgg cctgttcacc gccgcccaga tacaaggctg gaaacgcgtg
     1201 aacgacggcg tacatgccgc aggcggaaag atattcgtgc agctgatgca cgacgggcgc
     1261 gtcagccatc cggccaatat gctggccggc gcgaaggtgc tggcaccctc tgctatcgcc
     1321 gcaccgggcg agatgtggac ggacagcaag ggcatgcagc cctaccctgt tcctgccgag
     1381 atgaccgaag cagacatcaa acaagccatc gacgaattcg ccacggcggc aaagaacgcc
     1441 atcgaagccg gattcgatgg ggtcgagctg catggtgcga acggttacct gatcgaccag
     1501 ttcctcaata ccgccacgaa tcaacgcacg gatcgctggg gcggcagcgt cgagaaccgc
     1561 atccgcttcg ccgttgagat cgccaaggct gcggccgccg ccatcggtgc agaccgcatc
     1621 ggcatgcgca tctcccctta tggcgcattc aacggtgccg cacccgatgc cgagatggac
     1681 gcgatgtacc accgcttgat cagcgaactg aatgccatcg ggctggtgta tatccacatc
     1741 gtggatcaca gcgcgatggg cgcaccggaa gtcagcccca cgttaaaggc ctggatacgc
     1801 agcaacttca agggcaagta catcctctcg ggcggctatg acctcgctcg cgccaatgcc
     1861 gatctcgacg cgcagcgcgg cgacctcgtc gccttcggcc gcccgttcat ctccaacccc
     1921 gatctggtgc agaagatgaa gagcggcaca gcgctgaccg agcctgactt caataccttc
     1981 tacacccccg gagaaaaggg ctataccgac tattgattgt cattcgctct tgagcggtaa
     2041 cagcaaaaaa gccggcttac gccggctttt ttgtttactg cccgaacagc gcaataattt
     2101 tagtgcttgc tcttgtcgcc cttgatttcg gacagcaggc taggctcggt caccagcttg
     2161 cgcacgccgt gagcgtgatc ctctttgaag gcattaccct tgcaccactt ggcaacggag
     2221 ttgatgtcga gcttggcgca actcgggcgc acgctccagg tcacttgcag cggcgagcag
     2281 gtctggtcgt tgtacttggg cccggtggtc gaacccgcga acagaaccgg cttgccggtg
     2341 ttgcccggaa tcccgtcggc ctgatggtat ccgttgacca ccttgccgtg gtagtccagc
     2401 gtgttgaagt ccagcgcctt gggatcgttc accagcgtaa acacctggga ctccacgcgc
     2461 aggtcaggat tcttgacgtt gtcggtcaaa caggcgccca gcgtcgggcc tggcgtgact
     2521 ggagcggaag aatgcaccca gtgcacttcg atggtgtcgc ccggcttcag atcgccgtgc
     2581 tcgctcgggc agactggctc tgcggtcggc gccagttcgg ccgcgctcag gtgcttgctc
     2641 atgttgcact gatagccgcc atcggcgcca tgctcgcctt ccgcatgaat ggaaaattcc
     2701 ttcgccttgt gctcagcatt cacgtgaaag tgaatgttgc acagattcat ttccttgtaa
     2761 tccggtgcaa gcgagaagcc aatcttgttc tcgccatgct tgttgtcgat gtcgcgcgga
     2821 gtctgcggac cgaaaccggt acaagcaggc ccctcagtgc cgtgctctgc atgcatcgca
     2881 tcgtggccat gcacgtctgc ataaactgct gcggaaaaaa cggtggccgc gagcgcgacc
     2941 atgaattgct tcttgaacat tgatatctcc ctgagtcgat gtgtctattt gttatgttta
     3001 cgctcttgca agcgcaaacg cagtctagca gaccactggg gtcaacgcac acaccctaca
     3061 tcaggatgac atcatattgc tcttgtccgt acagggtctc gacctgcatc gatatcggct
     3121 tgccgatgaa gtccgaaagt tgcgccaagg cctgcgactc ttcatccaga aacaggtcga
     3181 cgacctgctg cgaggccaga atacggtatt cgcgcgcatt gaactggcgc gcctcccgca
     3241 ggatctcccg caagatctcg tagcacaccg tctgggcggt cttcacctcg ccccgcccct
     3301 gacaggtcgg gcagggctgg cacaacacat gcgccagact ctcgcgggtg cgcttgcgcg
     3361 tcatctccac caagccgagc gaggagaagc cgttcacgcc gatgcgggtg tgatcgcgcg
     3421 ccagcgcctt cttgaattca tccagcaccg catcgcgatg ctcctgcgta tccatatcga
     3481 tgaagtcgca gatgatgatg ccgccgagat tgcgcaaccg cagctggcgc gcaatgacct
     3541 gagtggcttc gagattggtc ttgaagatgg tgtcgtcgaa attgcgcagc ccgacaaaac
     3601 cgccggtatt cacgtcgacc gtggtcatcg cctcggtctg gtcgat
//
LOCUS       DASUIV010000248         9363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_342582, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000248 DASUIV010000000
VERSION     DASUIV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342582"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..862)
                     /locus_tag="VFW59_08980"
     CDS             complement(140..862)
                     /locus_tag="VFW59_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX5364393.1"
                     /translation="MKKSLLALSLMPLFGTAYAADFTDTARVVSSAPIYERVSEPRRE
                     CWTESVQVAPKERSLGGALIGGVAGGLLGSQVGGGNGSTVATGAGAVAGAVIGDRVAN
                     PDSNRSATGGIVGGVAGALLGSQIGGGTGNKAATAVGGVAGVVIGDRVANRDQPRTEQ
                     VERCRQVEDSREVVKGYNVTYRYNGQDVTTTLPYQPGSTVRVAVSIVQEPVAERRYDD
                     TQRRRYDDRSDRQERRYEDRRD"
     gene            complement(987..3770)
                     /locus_tag="VFW59_08985"
     CDS             complement(987..3770)
                     /locus_tag="VFW59_08985"
                     /inference="COORDINATES: protein motif:HMM:NF039312.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAHL domain-containing protein"
                     /protein_id="HEX5364394.1"
                     /translation="MNMLIVSFTENKKRVLGWLLTSVLLVLLFVNSRPFNQNYHDQIV
                     HHVAELSRYNSEQGELVLKQHFHLLNNFDALVSMSGQLQGELHGLRDSDALHALSDEP
                     AIMAAFKKMEARMLLEQSMLERFKSSNAVLRNSMLFLPHTVDELLNTMPPAVARRVGT
                     PLRRLRQDVVTISADASALDFVSLQDDLDEFRRVVATLPSGERASLELVGRHFEQVMR
                     LEAEASKLMRELTLSGKRDGISDEFAETFDAFYAREVRRSRRYLMLLFAVALAMMSYA
                     TYAFYHMRRLNERTQRALNEVRNQKVALDEHAIVTVIDMQGHITYVNQSLVAVSGYWQ
                     EELLGRDYRRIYSGDPDDEVLRDMQATLLRGQVWHGQMLNRAKDGHPYWVEATAVPFM
                     DATGSTYQHVLICTDITAQKEAEHEAMLAREAAEAASKAKGDFLAVMSHEIRTPMNGI
                     IGMTDLALDTDLSDIQRQYLSLVKSSADALLVIINDILDFSKVEAGKMVLEQVPFDIR
                     ELSDATTRLLAVRAAEKGVAVVARLDDGIPAVLLGDPGRLRQVLTNLLGNAIKFSRQG
                     EVTLRMRLLGRSGNKARLRIEVADQGIGIPAEKQVSIFEAFSQADASVTRQYGGTGLG
                     LAIASQLVTAMGGQLGVESEVGRGSVFGFEVEFSISDAALVESGAMVSHPSLPTAMMG
                     MNVLLAEDNAVNQKLAITLLERWGNRVTLANNGREAVELSREQSFDVILMDIQMPEMG
                     GFEATRLIREREARSGRYTPIVAMTANAASEDRQRCLDAGMDDYIAKPIAAAQLLALL
                     QGRSAMAHQAVAPIADSSAPFDYAEAITRADPDVVESIGQAFLDSCDQTLDEIVSAIA
                     ANDDKTLSRGAHTLRGLAGYFNAARIETLSRQLEGISERGDWSQASGISRHLQGEVAL
                     LKTALAAHLANSR"
     gene            complement(3767..4834)
                     /locus_tag="VFW59_08990"
     CDS             complement(3767..4834)
                     /locus_tag="VFW59_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEX5364395.1"
                     /translation="MSFDRPRLRRLSVTLLHDRRLLLALFMLVLFGSLLFFLYIPSLP
                     VKTQPDAVYGDEVVGTVEPIVPIPLEIKLDKNKVALGMRLFHDPRLSGDNTVSCANCH
                     GLDHGGADHAIHSRGVGGQEGVINAPTVFNSGFNFRQFWDGRAETLEEQIDGPINNPI
                     EMASNWPQVVSRLSADVEYKKTFSRIYSTGVTAANIKDAIATFERSLYTPNSRFDRFL
                     RGDKAALNADEEAGYRLFKTIGCVSCHQGVNVGGNLYEKMGVMEGYYVTGSVVTKSDL
                     GRYNQTGLDEQRYEFKVPSLRNVALTGPYFHNGSADTLEEATRLMAWHQLGVKLGAAE
                     QRLIADFLRTLTGEYQGVALK"
     gene            complement(4824..4988)
                     /locus_tag="VFW59_08995"
     CDS             complement(4824..4988)
                     /locus_tag="VFW59_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364396.1"
                     /translation="MKTTDSDGRMKMNVWDDMQIKAALISQRVAVGFIVGARGDRVRS
                     RSDEGARHVI"
     gene            5198..6010
                     /locus_tag="VFW59_09000"
     CDS             5198..6010
                     /locus_tag="VFW59_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364397.1"
                     /translation="MSFLTGIHDLNVFVVEPSAVQSQFIDSMLKSIGIQLPTLFKDGN
                     SALQAMRANRPDVVISSMYLPDMTGGELVEHLRADNWTSGIAFILVSSESNPHHLEPV
                     RQGGASAILGKPFTDEQLKQALSTTLDYLNPDAERIEVSGEVDLEAIKVLLVDDSRSA
                     RKFMRHVLENLGIRNFTEAENGKQAVDLIQQHYFDLVVTDYNMPEMDGRELTEYIRTQ
                     SWQSTVPILMVSSESDQSRLAAVVQAGVSGVCDKPFEPALVRGLLEKMLRVD"
     gene            6063..6398
                     /locus_tag="VFW59_09005"
     CDS             6063..6398
                     /locus_tag="VFW59_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5364398.1"
                     /translation="MTITVQLTDNKARITIAGRFDSSSYKEFKQSYTPVIENPAIRIV
                     EVDVSGVEYLDSAALGMLVQLNEHAKNTKKSITLVSVPGRVSDILRTASADKLFTINL
                     PSGIKMDLR"
     gene            complement(6465..6968)
                     /locus_tag="VFW59_09010"
     CDS             complement(6465..6968)
                     /locus_tag="VFW59_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364399.1"
                     /translation="MTTSSVRPKIMVVDDTPLNLNLMDSILGADYSVQLVSSGAKAIE
                     LATAAAPDLILLDVMMPEMDGFEVCRRLKANPATGHVPVIFVTAKREIADEKLGFEVG
                     ASDFIHKPVSAPIVAARVRTHLKIKFMQDYLQNENLRLQENAEQNSSELEQLKGFIWG
                     APNFARR"
     gene            complement(7172..8293)
                     /locus_tag="VFW59_09015"
     CDS             complement(7172..8293)
                     /locus_tag="VFW59_09015"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5364400.1"
                     /translation="MKLNVMVVDDTQINVTLISHLISKLEDCVAAGYTVPEQGLASCE
                     AQIPDLLIVDYMMPGLDGIEFIRRFRALPGREDIPVLMVTANDQIEVRHQALNVGAND
                     FLTKPIDKIEFMARVRNMLALRRSQRRLERSNLILNDEKELLEDIVTRMRSSSPFDTR
                     GVRYIQSSLERTAGDIVLSAYRPDGAQHVLVGDFSGHGLPAAFGAPLASYIFYQLTAE
                     GRDLRCILDEMNRTLYRQLPTQLYMAASALELSSDRKLARLWNYGLPPTLCLSAVHAM
                     SRVDSKGLPLGIAESLDDFEPHAELAVVPGMCIYQYSDGITEAASPEQELYGQERLVE
                     LVARIYREQLPLEVIWTELERYCAGQGLSDDAAMVEVAV"
     gene            complement(8315..8953)
                     /locus_tag="VFW59_09020"
     CDS             complement(8315..8953)
                     /locus_tag="VFW59_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5364401.1"
                     /translation="MELLTAFIDLVLHLDAHLLKLTADYGMWVYGILFLIIYCETGLV
                     VTPFLPGDSLLFVAGALCGMGALQIEWLIPLLILAAFSGDNTNYWIGRLIGMRLLHRT
                     NGLIKREHIDKTHAFYEKHGGKTILFARFLPIVRTFAPFVAGIGLMRYRLFLAFSALG
                     SVAWIGGLTVAGYFFGNIPLVKNNLTLIIIGIIVITLLPALRELIRHRKQLR"
     gene            complement(8957..>9363)
                     /locus_tag="VFW59_09025"
     CDS             complement(8957..>9363)
                     /locus_tag="VFW59_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5364402.1"
                     /translation="GFFAFFQWEFSLSVLAAVLAVLGYSVNESVVVFDRIRENFRSMR
                     KAEVTEIIDNAITRTISRTIITHGCTQMMVGAMLFLGGETLHYFAMALTIGILFSIYS
                     SVLVASPLLMLLGVSREDFIKPEKTEAEEVLP"
BASE COUNT         2062 a         3003 c         2486 g         1812 t
ORIGIN      
        1 acctgccggt aatgtaacgg tgaagactaa tgcgcatagc gcgttacgtc gaaaccgcta
       61 catcacagga cgattcggtt aatcgtccag cccccaacaa aaaagcccgg taaaaaccgg
      121 gctttttttg aatcaacgat cagtcacgac gatcctcgta acgacgctcc tgccggtcgc
      181 tgcggtcatc gtaacggcgg cgttgcgtat cgtcatagcg acgctccgca accggctcct
      241 gaacgatgct cactgccact cgcacggtcg agcccggctg gtaaggcagg gtggtggtga
      301 catcctgacc gttatagcgg taagtcacgt tgtagccttt gacaacctcg cggctgtctt
      361 ccacctgacg gcaacgctcg acttgctcgg tacgcggttg atcgcggttg gcgacacgat
      421 cgccgatcac gacaccggcg acaccgccca cagcggtggc ggccttgttg ccggttccgc
      481 cgccgatctg actgcccagc aacgcaccgg ccacgccgcc gacgattccg cccgttgcgg
      541 aacgattgct gtcggggttg gcgacacggt ctccgatcac agcgccggcc accgcaccgg
      601 caccggtcgc gaccgtactg ccgttgccgc cgccaacctg actacccagc aacccgccgg
      661 ccacaccacc gatcaacgcg ccgcccagcg agcgctcctt gggtgcgacc tgcaccgact
      721 ccgtccagca ctcccggcgc ggttcgctca cgcgttcata gatcggcgcg ctggaaacaa
      781 cgcgtgcggt atcggtaaaa tcggcagcat aagcggtgcc gaacaagggc atcagactca
      841 aggcaagcaa actcttcttc attttcatca ctcctgaaca gtcccgcaca tacgagactg
      901 caatcagacc gctcccaagc cggcaggttc catttgcgcc gttcgcggga ttccagtcac
      961 gcctcgctgg cggctggcac aatccgctat cggctgttcg ccagatgcgc cgccaatgcc
     1021 gttttgagca gcgccacttc gccctgcaaa tgccgactga taccgctcgc ctgagaccag
     1081 tcgccacgct cggagatacc ctcaagctga cgcgacagcg tctcgatgcg agcggcattg
     1141 aagtagcccg ccagtccgcg cagggtatgc gcgccgcgcg acagcgtctt gtcgtcgtta
     1201 gccgcaatcg cactgacgat ctcatccaga gtctgatcgc aggaatcgag gaaggcctgt
     1261 ccgatactct ccaccacatc cgggtcggcg cgcgtgatgg cttcggcata atcaaaaggc
     1321 gccgaactgt cggcgatggg cgctacggcc tgatgcgcca tggctgacct gccttgcagc
     1381 aaggccagca actgggctgc ggcgatgggc ttggcgatgt agtcgtccat ccccgcatcc
     1441 agacaacgct gcctgtcctc acttgccgca ttggcggtca tcgcaacgat gggggtatat
     1501 ctgccactcc gggcctcgcg ctcgcgtatc aatcgcgtcg cttcaaaacc gcccatttct
     1561 ggcatctgga tatccatcaa aatcacatca aagctctgct cacggctcag ctcgaccgcc
     1621 tcgcggccat tgttcgcgag cgtcacgcgg ttcccccaac gttccagcag ggtgatcgcc
     1681 agcttctgat tgaccgcatt gtcctcggcc agcaagacat tcatgcccat catcgcggtc
     1741 ggcaacgagg ggtgactgac catcgcgcca gactcgacca acgcggcatc gctgatcgaa
     1801 aactcgacct cgaaaccgaa tacgctcccc cgtccaacct cgctctcgac ccccagctgc
     1861 ccgcccatcg ccgtcaccag ctggctggcg attgccagcc ccagaccggt tccgccgtac
     1921 tgccgggtga ccgatgcatc cgcctgactg aaggcctcga agatgctgac ttgcttctcg
     1981 gcggggatgc ctatcccttg atcggccacc tcgatgcgca gccgcgcctt gttgcccgaa
     2041 cgcccaagca gcctcatgcg cagcgtcacc tcgccttgac ggctgaactt gatcgcattg
     2101 cccagcagat tggtcagcac ctgacgcaaa cgtccgggat cgcccagcag cacggcgggg
     2161 atgccgtcat ccagacgcgc aaccaccgcg acccccttct ctgccgcacg taccgcgagc
     2221 aatcttgtcg tcgcgtccga caattcccgg atatcgaacg ggacctgttc cagcaccatc
     2281 ttgcccgcct caaccttgga gaaatccaga atatcgttga tgatcaccag caacgcatcg
     2341 gccgacgatt tcaccaagga caggtattgg cgctgaatgt cgctcaagtc ggtatccaga
     2401 gccagatcgg tcatgccgat gatgccgttc atcggcgtgc gtatctcgtg gctcatcacc
     2461 gccaggaaat cgcctttcgc cttggatgcg gcctcggccg cctcccgggc cagcatcgcc
     2521 tcatgctcgg cctccttctg cgcggtgata tcggtacaga tcagcacatg ctgataagtg
     2581 ctgcccgtcg catccatgaa ggggaccgcc gttgcctcga cccaataggg atgtccgtcc
     2641 ttggcccggt tcaacatctg accgtgccag acttgtcctc gcaacagggt ggcctgcata
     2701 tcgcgcaaca cctcgtcgtc cgggtcgccg gaataaatcc ggcggtaatc cctgcccagc
     2761 aattcttcct gccaataacc gctgacggcg acgaggctct gattgacgta ggtgatatgc
     2821 ccctgcatgt cgatgaccgt cacgatagcg tgctcatcca gtgcgacctt ctggttcctg
     2881 acttcgttca aagcccgctg ggtacgctca ttgagccggc gcatgtggta gaaagcatag
     2941 gttgcataag acatcatcgc cagcgccacg gcgaacaaca gcatcagata gcggcgcgaa
     3001 cgccgcacct cacgcgcata gaacgcatcg aatgtctcgg cgaattcatc gctgatacca
     3061 tcccgcttgc cggaaagcgt caattcgcgc atcagcttgc tggcttcggc ttcaagcctc
     3121 atcacctgct caaagtgcct tcccaccaat tccagtgacg cacgctcacc cgatggcaac
     3181 gtggcaacca cccgcctgaa ctcatccaga tcatcctgca aggacacgaa atcaagcgcc
     3241 gaggcatcgg cactgatggt cacgacatcc tggcgcaggc gccgcagcgg tgttccgacc
     3301 cgccgcgcga cagcgggagg catggtgttg agcagctcgt ccaccgtatg cggcagaaac
     3361 agcatggaat tgcgcagcac cgcattgctg gatttgaaac gctccagcat gctctgttcc
     3421 agcagcatgc gcgcctccat cttcttgaac gcagccatga tcgccggttc gtcgcttaac
     3481 gcatgcagcg catcgctatc gcgcaaaccg tgcaactccc cttgcaactg ccccgacatg
     3541 ctcaccaggg catcgaagtt gttcagcaga tggaaatgct gcttgagcac cagctccccc
     3601 tgttcggagt tatagcggct cagctcggcg acatgatgca cgatctggtc gtggtagttc
     3661 tgattgaaag ggcgcgaatt gacgaacaag agcaccagca agacgctggt caacaaccac
     3721 cccagcacac gctttttatt ctcggtgaaa ctgacgatca gcatgttcat ttcagcgcca
     3781 ctccctgata ctcgcccgtc agcgtgcgca ggaaatcggc gatcaggcgc tgttctgctg
     3841 cgcccaattt cacccccagc tggtgccagg ccatcagacg cgttgcctct tccagcgtat
     3901 ccgccgaacc attgtggaaa tacggcccgg tcaaagccac attacgcaaa gaaggcacct
     3961 tgaactcgta gcgctgctca tcgagtccgg tctggttgta acgccccagg tcgctcttcg
     4021 tcacgacgct gcccgtcacg taataccctt ccatcacccc catcttttcg tacagatttc
     4081 cgcccacatt cacgccctga tgacaggaca cgcagcctat cgtcttgaac aaccggtagc
     4141 cggcttcttc gtcggcattc aacgccgcct tgtcgccacg cagaaagcga tcgaaacgcg
     4201 aattcggggt atacagcgag cgctcgaaag tcgcgatggc atccttgatg ttggccgcag
     4261 tcacccctgt cgaatagatg cgggaaaagg tttttttata ctcgacatcc gcactcaatc
     4321 tgctcacaac ctgcggccag ttactcgcca tttcgatcgg attattgatc gggccatcga
     4381 tctgctcctc cagcgtctcg gccctgccat cccagaattg ccggaaattg aagccgctgt
     4441 tgaacacggt cggcgcattg atcaccccct cctgtccgcc gaccccgcgc gaatggatcg
     4501 catgatcggc tcctccatga tcgagtccat gacagttcgc acaggacacc gtgttatcac
     4561 cggagagcct cgggtcatgg aacagtcgca tgccgagtgc cactttgttc ttatccagtt
     4621 tgatttcgag cggtatcggc acgatcggct cgaccgtccc gaccacctca tctccgtaaa
     4681 ccgcatcagg ctgcgtcttc accgggagtg acggtatata cagaaaaaac agcaatgagc
     4741 cgaacagaac cagcatgaac aacgccaaca gcaacctgcg gtcatgcaac aacgtaaccg
     4801 acaagcgcct caggcgcggc cgatcaaatg acatgacgcg ctccctcgtc agatctggaa
     4861 cgcaccctgt caccccttgc gccgacaata aaaccgacag caacccgctg cgagatcaac
     4921 gcggccttga tctgcatatc gtcccacaca ttcattttca tcctgccatc actatctgtc
     4981 gttttcatga accgatctct ttcgttcgcc ttgatcgcaa ccgcgtgaat accagagaga
     5041 cgctcgtctt ccgatgacgg cgagcaccct gcacgacaac caaccgcata ataaacatgg
     5101 gttctattca ctggcgtttg cctgctgtat ttgcaagaat agcatccttc cgttctcaca
     5161 cttgaatctc ggtggagaac ctttaaaagg aatcagcatg agttttctta ccggcatcca
     5221 cgaccttaac gtgttcgtgg ttgagccctc cgcagtccag tcccagttca tcgattcgat
     5281 gctgaagagt atcggcatcc agctgcccac gctgttcaaa gacggcaaca gcgcgctgca
     5341 ggcgatgcgc gccaaccgtc cggatgtcgt catcagttcc atgtacttgc cggacatgac
     5401 cggaggtgaa ctggtggagc acttgcgcgc cgacaattgg accagcggca tcgctttcat
     5461 tctggtctcc agcgaatcca acccccacca tctggagccg gtacgccagg gcggagcaag
     5521 cgcaattctc ggcaaaccat ttacggacga acaactgaaa caggctttat ccaccacgct
     5581 cgactacctg aatcccgacg ccgaaagaat cgaggtgagc ggcgaggtcg acctagaggc
     5641 catcaaggtg ctgctggtgg acgacagccg cagcgcccgt aaattcatgc gccacgtact
     5701 ggagaacctt ggcatccgga atttcaccga ggccgagaac ggcaagcagg cagtcgacct
     5761 catccagcag cactatttcg acctggtggt gaccgattac aacatgccgg aaatggatgg
     5821 gagggagttg accgaataca tccgcaccca aagctggcaa agcaccgtac ccatcctgat
     5881 ggtgtcgagc gaaagcgacc aaagccgtct ggcagcggtg gtgcaggcgg gcgtatcggg
     5941 cgtgtgcgac aaaccgttcg aacccgcctt ggtaagaggc ctgcttgaaa aaatgctgcg
     6001 cgtcgattag cctgccaggc acgcaacgct ttacagaaca ccttctaatt caggagtcat
     6061 gcatgaccat taccgtacag ctcaccgaca acaaggcacg gatcaccatt gcgggtcgtt
     6121 tcgactcaag cagctacaag gaattcaagc agtcctacac cccggtgatc gaaaacccgg
     6181 cgatacgcat cgtggaggtg gacgtaagcg gagtggagta tctggacagc gcggcgctgg
     6241 gtatgctggt acagctgaac gaacatgcca agaacaccaa aaaatccatc acgctggtca
     6301 gcgttcccgg gcgtgtatcg gacatcttga gaacggcgag cgccgataag ctgttcacca
     6361 tcaacctgcc atccggaata aaaatggatc tgcgctaaaa agcgcgcgcc ccccgattcc
     6421 gtgaccgatc gacaatcagg ctgggcgagc cgccacgaac cgccttaacg tctggcgaag
     6481 ttaggcgcgc cccagataaa ccccttcaac tgctccagct cggaagaatt ctgctcggcg
     6541 ttttcctgaa gccttaagtt ctcgttctgc aaataatcct gcatgaactt gatcttgagg
     6601 tgggttcgca cgcgtgcggc cacgataggc gcactgacgg gcttgtggat gaaatcggat
     6661 gcgccgacct cgaatccaag cttctcatcc gcaatttccc gcttcgccgt gacgaagatg
     6721 acgggcacat ggccggtcgc agggttggcc ttcagacggc ggcacacttc gaatccgtcc
     6781 atctcgggca tcatcacgtc cagcaggatc aaatctggcg cagcagcagt cgccagttcg
     6841 atcgccttcg cgccgctact gaccagctgc acgctgtagt ccgcccccag aatgctgtcc
     6901 atcagattca gattcagcgg cgtatcgtcc accaccatga ttttaggacg tacggaagaa
     6961 gtggtcatcg cattctctct attgttaact tatccgtctg aacggcttgc cgcctcagac
     7021 gcaatcaaat cgcagccgtc ccgtcatggg cgctgcgccc ttgctcgctc atccactttc
     7081 cctgtcgtac gaccggcaga tgaaagcgcc cgcgatttta ctacaagaca aacttgcccc
     7141 gaacttgcgt tcaaataaat ccgcgcattg actatacggc aacctcgacc atcgcagcat
     7201 catcggacaa tccttgtccg gcgcaatacc gctccaactc tgtccaaatc acttccagcg
     7261 gcaattgctc acggtagata cgcgcgacca actcgaccag tctctcctgc ccatagagct
     7321 cctgctcggg cgaggcggct tcggtgatgc catccgagta ttgatagatg cacataccgg
     7381 gcacaacagc cagttcggca tgaggctcga agtcgtcaag cgattcggcg attcccaacg
     7441 gcaagccctt ggagtccact cgcgacatgg cgtggacggc actcagacaa agcgtgggag
     7501 gcaaaccgta attccacagt cgcgccaact tgcgatcaga ggaaagctcc agcgcgcttg
     7561 ccgccatgta aagctgggtt ggcagctgac ggtatagcgt gcggttcatc tcgtccagaa
     7621 tgcagcgcaa atcgcgcccc tcagccgtca gctgatagaa gatataagaa gcaagcggcg
     7681 caccgaacgc cgcaggcaga ccatgcccgg agaaatcacc caccagtaca tgctgcgcgc
     7741 catccgggcg ataagcggac agcacgatgt cgcccgccgt ccgctccagt gacgactgga
     7801 tatagcgcac tccgcgcgta tcgaacgggc tggacgaacg catcctggtc acgatgtctt
     7861 ccagcaattc cttttcgtcg ttgaggatca ggttgcttct ttccaggcgg cgctgactgc
     7921 gccgcagtgc cagcatgttg cgcacgcgcg ccatgaattc gatcttgtcg atgggcttgg
     7981 tcagaaagtc attggcaccg acattcagcg cctgatggcg cacctcgatc tggtcgttgg
     8041 ccgtcaccat cagcaccgga atatcctccc gtccgggaag cgcgcggaag cggcggatga
     8101 actcgatgcc atccagcccc ggcatcatgt aatcgacaat gagcaaatcc ggaatctgcg
     8161 cttcgcacga cgccagcccc tgctcgggga cggtgtaccc ggcagcgaca caatcctcca
     8221 gcttggagat cagatgagaa atcagggtga cgttgatctg cgtatcgtcc accaccatca
     8281 cgttcaattt cattcgaccc cctcaacaaa agactcagcg gagttgcttg cgatgcctga
     8341 tcagttcacg caacgcgggc agcagcgtga tgacgatgat accgatgatg atgagggtga
     8401 gattgttctt caccagaggg atattcccga agaaatagcc cgccacggtg aggccgccga
     8461 tccacgccac actgcccagc gcactgaagg ccaggaacag gcgataacgc atcaggccga
     8521 taccggcgac gaagggcgcg aaggtgcgca cgatgggcag gaagcgcgca aacagaatgg
     8581 tcttgccgcc gtgcttctcg tagaaggcgt gggtcttgtc gatatgctcg cgcttgatca
     8641 ggccgttggt gcggtgcaac agacgcatac cgatcaggcg cccgatccag tagttggtgt
     8701 tgtcgcccga gaacgccgcc aggatcagca aggggatcag ccattcgatc tgcaatgccc
     8761 ccatgccgca cagcgcaccc gccacaaaca gcagcgaatc gccgggcagg aagggcgtca
     8821 ccaccagacc ggtctcgcaa tagatgatca gaaacaggat accgtacacc cacatgccgt
     8881 agtcggcggt gagtttcagc agatgcgcgt ccagatgcag gacgaggtcg atgaaagcgg
     8941 tcagtaactc catggcttaa ggcagcactt cttctgcttc ggtcttctcg ggcttgatga
     9001 agtcctcgcg cgacacgccc agcagcatca gcaagggact cgcgaccagc accgaggaat
     9061 agatgctgaa cagaataccg atggtcagcg ccatcgcgaa gtaatgcagc gtctcgccgc
     9121 cgaggaacag catcgcgccg accatcatct gggtacagcc gtgggtgatg atggtgcggg
     9181 agatggtgcg ggtgatcgca ttatcgatga tctcagtcac ctcagccttg cgcatggagc
     9241 ggaagttctc gcggatacga tcgaacacca ccaccgattc gttcaccgaa tagcccagca
     9301 ccgccagcac cgccgccagt accgacagcg agaactccca ctggaagaag gcgaagaagc
     9361 cga
//
LOCUS       DASUIV010000249         5501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_991625, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000249 DASUIV010000000
VERSION     DASUIV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991625"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="gspF"
                     /locus_tag="VFW59_09030"
     CDS             <1..1179
                     /gene="gspF"
                     /locus_tag="VFW59_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEX5364403.1"
                     /translation="DAAGRSVSGVIQSDTARQVRAQLRSQGLLPSTITQVHARERAGQ
                     LWARGISAAELSLVTRQMATLLDSGLTMEQSLNALIEEAAEPMTREILIGVKTEVTSG
                     QSLASALGAYEKSFPDFYRALVNGGEESGALPTVLQHLADYLDARQALKQKTSLALLY
                     PVLVTVVAIMIVTGLLIYVVPQVVQVFQQSRQSLPLLTRTLIALSDFLRAAWPYLAVL
                     GVGGFAAARMALRQAATRRRWHGLLLNMPGLGLLIRGINTSRFASTLAILVGGGVPLL
                     SALASGARVMTNMVLREAVEHAIERVREGSGLARALGETHAFPPLLIHLIASGEVSGK
                     LEQMLKRAAQLETQALERRLTVFLTLLEPVMILVMGGIVLLIVLAILLPIIQINQLVH
                     "
     gene            1264..1743
                     /locus_tag="VFW59_09035"
     CDS             1264..1743
                     /locus_tag="VFW59_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5364404.1"
                     /translation="MKKILIAAAISAAFAAMPAAAQGYVGIGAGVANTDANHTSYKAF
                     AGIQANQNVGVEIAYNDFGNYRGAWAESWSVAGVGTLPLNETWDVIGKLGVTSNHANF
                     AGSSRHSDILVGLGVAYNFAPNMAVRLEYEDFGNLPAAINGVISKANNLGLNLKYSF"
     gene            complement(1906..2202)
                     /locus_tag="VFW59_09040"
     CDS             complement(1906..2202)
                     /locus_tag="VFW59_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5364405.1"
                     /translation="MNTQENNPAVTCATCLACCCRLEVMLMADDDIPVELTEQDRWGG
                     WVMARLDDGWCAALDRGTMLCTIYSRRPMICREYQAGDSDCIGERILHRIDTAS"
     gene            2314..3096
                     /locus_tag="VFW59_09045"
     CDS             2314..3096
                     /locus_tag="VFW59_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364406.1"
                     /translation="MLTEWLTYLTTDCSPSARELGYLRESIGIRSRYRRCKAAWQPHL
                     KHSRAALLESLHACNDFRTALVFGSGLLLDIPLTELAARFERVYLVDVVHLPEVRRIA
                     RRHANVQCVSHDITGFMEHREALSPDRLDLPPPQRFLDDPSIDWVASVNLVSQLPLLP
                     QDWLRQRFPELDETTLDAWGTRLMRQHLDYLRAFAAPVCLLADREQTIHAREGEVLEQ
                     IDFTARLGLADSPMRQWRWDIAPPGEIAPDTGSYHLVSVHTL"
     gene            3158..4288
                     /gene="zapE"
                     /locus_tag="VFW59_09050"
     CDS             3158..4288
                     /gene="zapE"
                     /locus_tag="VFW59_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX5364407.1"
                     /translation="MSPHNLPGDNSVSPKSWYRAASAQPDFSPDPAQAAAIEELELLW
                     HKLVDFKARRNQFLGRSLLSPDVPKGLYLWGGVGRGKTFLMDAFYASLPYRRKRRIHF
                     HNFMAEVHHEMKLLAHADDPLVALAQRISDSTRLLCLDEFHVDDIADAMILGRLVSEL
                     FKRGVVLMTTSNYPPDGLYPHGLQRQNFLPAIDLIKRELKILHVDGGNDYRTQAMTRE
                     PLFLVPADEANEARMEALFERIAAGIPQRSIEVRGRMLPVKRVARSVVWFDFSELCGG
                     AHAQEDYLAIAQRYPTLFLSDIPKMTSAHAAEMRRFIWLIDVLYDHNVRLVASAAATP
                     EQLDAEGVTAGEFSRTASRLSEMQSQRYLQLPHRAQGVTLNE"
     gene            complement(4347..>5501)
                     /gene="tldD"
                     /locus_tag="VFW59_09055"
     CDS             complement(4347..>5501)
                     /gene="tldD"
                     /locus_tag="VFW59_09055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX5364408.1"
                     /translation="AQATRAIARQGGKQKVPMLHRGARCAIYLPNDPIASMKDADKVA
                     LLERIERYARAIDPRVTQVMAGLAGEYEVVMVARSDGLMNADIRPLVRLSVQVIVEAN
                     GKREQGSAGGGGRFDYDYFDDAMLHRYAEQAVHQAVINLDAGPAPAGEMTVVLGSGWP
                     GILLHEAIGHGLEGDFNRKGSSAFSGRVGERVAAKGVTVVDDGTIASRRGSLQMDDEG
                     NQTQCTTLIEDGILKGYLQDSLNARLMGVPVTGNARRESYAHLPMPRMTNTMMLNGDK
                     TREEIIASVKHGLYAVNFGGGQVDITNGKFVFSTTEAYLIEDGKITRPVKGATLIGNG
                     PDALTRVAMIGNDMALDSGVGTCGKEGQSVPVGVGQPTVRIDGLTVGGTA"
BASE COUNT         1035 a         1769 c         1610 g         1087 t
ORIGIN      
        1 gatgccgccg gccgcagcgt atccggcgtg attcaatccg atacggcccg tcaggtacgc
       61 gcgcagttgc gcagccaggg actgctgcca tcgacgatta cccaggtgca tgcgcgtgaa
      121 cgcgccggtc aattatgggc acggggcata tcggctgccg aactcagtct ggtgacccgt
      181 cagatggcga cattgctcga ctccggcctg accatggagc agtcgctcaa tgccctgatc
      241 gaagaagccg ccgaaccgat gacgcgcgaa atactcatag gcgtcaaaac cgaagtgaca
      301 tccggtcagt cgctggccag cgcactgggg gcttacgaaa aaagctttcc ggacttctac
      361 cgcgcgctgg tgaatggcgg cgaggaatcc ggtgcgctgc cgaccgtatt gcaacatctg
      421 gccgactacc tcgacgcgcg ccaggcattg aaacagaaga ccagcctcgc gctgctttac
      481 ccggttctgg tcacggtcgt ggcgatcatg atcgtcaccg gcctgctgat ctacgtggtc
      541 cctcaggtgg tacaggtgtt tcaacaatcg cggcaaagcc tgccgttgtt gacgcgcacg
      601 ctgatcgcac tcagcgattt tttgcgtgcg gcctggccgt atctcgcagt ccttggcgtt
      661 ggcggatttg ccgccgcccg catggcactg cggcaagccg cgaccagacg ccgctggcac
      721 ggcttgcttt taaatatgcc gggactgggc ttgctgatac gcggcatcaa tacctcgcgc
      781 ttcgccagca cgctcgccat tctggtcggc ggcggcgtgc cgctgctttc cgcactggct
      841 tcaggtgcac gcgtgatgac gaatatggtt ctgcgtgaag cggtcgagca cgccatagag
      901 cgggtgcgcg aaggcagcgg actggcccgc gctctgggcg aaacccatgc cttcccgccg
      961 ctgctgatcc acctgatcgc cagcggcgag gtcagcggca agctggagca aatgctgaaa
     1021 cgtgccgcac agctcgaaac tcaggcactg gagcgacgcc tgacggtctt cctgacgctg
     1081 ctcgagcccg tgatgatact ggtgatgggc ggcatcgtgc tgctcatcgt gctcgccatc
     1141 ctgctcccta tcattcagat caatcagctg gtgcactgag tttacgtgtg tcaacgcaca
     1201 tacctgtctg gcatagcccc gtatattcaa catcccataa tgatcattta caggtaaaca
     1261 cccatgaaaa agattctaat tgcagcagca atcagtgccg cattcgcagc gatgccggcg
     1321 gccgcacaag gctacgtagg tattggagcc ggcgttgcca ataccgatgc caaccatacc
     1381 tcctataaag catttgcggg catacaagcc aaccagaatg tcggcgttga gatcgcctac
     1441 aacgattttg gcaattaccg cggcgcttgg gccgaatcct ggtctgtcgc tggcgttgga
     1501 acgctgccat tgaacgaaac ctgggatgtg atcggcaagc tgggcgtcac ctcaaaccat
     1561 gccaatttcg cgggttcctc gcgtcattcg gacattctgg tcggtttggg cgttgcttat
     1621 aactttgctc ccaacatggc ggttcgcctc gagtacgaag atttcggcaa tcttccggcg
     1681 gcaatcaatg gcgtaatttc aaaagcaaac aatctgggcc taaacctgaa gtactccttt
     1741 taaagaaggc tggcccctgt tgaaaatcag gggttagcca taccccttta atcgagcgtg
     1801 tagtgttttg atggcgccag accggttgaa tccgcaaccc atttcggtca tttgccttgc
     1861 ccgaaagccg gtcgcaattg cgattttgtg tgtcaagcga accgcttatg acgcagtatc
     1921 gatacggtgc agaattcgct ccccgataca gtcgctgtca cccgcctggt attcgcggca
     1981 gatcatcggg cggcggctat agatcgtaca cagcatggtt cccctgtcca gagcggcgca
     2041 ccagccgtca tccagtcgcg ccatgaccca tccgccccac cgatcctgtt ccgtcaattc
     2101 gacgggaatg tcgtcgtcgg ccatcagcat cacctccagg cggcaacagc aggctaggca
     2161 ggtggcgcag gttacggccg gattgttctc ttgggtattc atccttggca ttttcgcacc
     2221 ttatcaagcc gcgaccaacc gctgctgata gaagaggccc gcgatcgcat gtcgcgccaa
     2281 cccataccgc acgggttcgt attaccatgc gccatgctga ccgagtggct tacttacctg
     2341 acgaccgatt gctcgccctc tgcacgcgaa ctgggctatc tgcgcgagag catcggcatc
     2401 cgctcgcgct accggcgctg caaagcggca tggcagccgc atctaaaaca cagccgcgcc
     2461 gccttgctgg aatcgctgca tgcctgcaac gactttcgca ccgcgctggt cttcggttcc
     2521 gggctgttgc tggatattcc gctgaccgag ctggccgcac gcttcgagcg cgtttatctg
     2581 gtcgatgtcg tccatttgcc cgaggtgcgc cgcatcgcgc gccgtcatgc caacgtgcaa
     2641 tgcgtctcgc acgacatcac cggattcatg gaacacaggg aagcactgtc gcccgaccgc
     2701 ctggatctgc cgccgccgca acgttttctc gacgaccctt ccatcgactg ggttgcctcg
     2761 gtcaatctgg tctctcagtt accgctattg ccgcaggact ggctgcgtca gcgctttccc
     2821 gaactcgacg aaacaacact ggacgcatgg ggtacgcggc tgatgcggca gcatctggac
     2881 tacctcaggg ccttcgctgc acccgtctgc ctgctggcag acagggaaca aacgatccat
     2941 gcgcgggagg gcgaggtgct cgaacagatc gactttaccg caagactcgg cctagccgac
     3001 tcgccgatgc ggcaatggcg ctgggacatc gcaccgcccg gagaaatcgc accggacacc
     3061 ggcagctacc acctcgtgtc ggtgcatacc ctataattgc agccgtcgta tgaccatcgt
     3121 cacgcatccg cgcccaccat taagccgaaa acccaccatg tccccgcaca acttgcccgg
     3181 cgataacagc gtcagcccca agagctggta tcgggccgcc agcgcgcagc cggactttag
     3241 ccctgatccg gcacaggcgg cggccatcga agagctggag ctgctttggc ataagctggt
     3301 ggatttcaag gccagacgca accagtttct ggggcgcagc ctgctcagcc cggatgtgcc
     3361 caagggcctg tatctgtggg gcggcgtcgg acgcggcaag acgttcctga tggatgcctt
     3421 ctacgcgtcg ttaccctatc gccgcaaacg ccgcatccac ttccataact tcatggccga
     3481 ggtgcatcac gagatgaaac tgctggccca tgcagacgac ccgctggtcg cgctggccca
     3541 gcgcatttcc gactccaccc ggctgttgtg tctggatgaa ttccacgtcg acgacatcgc
     3601 cgatgcgatg atcctggggc ggctcgtcag cgagctgttc aaacgcggcg tggtgctgat
     3661 gaccacgtcc aattacccgc ccgacggcct gtatccgcac ggcctgcaac ggcagaactt
     3721 cctgcccgcg atcgatctga tcaaacgtga gttgaagata ttgcatgtgg atggcggcaa
     3781 cgactaccgc actcaggcga tgacccgcga gccgctgttt ctggtgcctg ccgatgaggc
     3841 gaacgaggcg cgcatggagg ccctgttcga gcgcatcgcc gccggcattc cgcaacgcag
     3901 catcgaagta cggggacgca tgctgccggt gaagcgcgtc gcgcgcagcg tggtctggtt
     3961 cgatttcagc gagctatgcg gcggcgctca tgcgcaggaa gactatctgg ccatcgccca
     4021 acgctacccc acgctgttcc tgtcggatat accgaagatg acctccgctc acgcagccga
     4081 gatgcggcgc ttcatctggc tgatcgacgt gctgtacgac cacaacgtca gactggtcgc
     4141 ctcggcggcg gcgacaccgg aacagctcga tgccgaaggc gtgaccgcag gcgaattctc
     4201 ccgcaccgcc agccgcctgt ccgagatgca gtcgcagcgc tatttgcaac tgccgcaccg
     4261 cgcgcagggc gtgacgctga acgaataatc gcccggttag aatccgctga ataaaagcgg
     4321 aacaggatcg ttccgcctta agagagttac gccgtgccgc ccaccgtcag gccgtcgatg
     4381 cgcaccgtgg gctggcccac gccgaccgga acgctctggc cttccttgcc gcaggtaccc
     4441 acgccggaat cgagcgccat gtcgttgcct atcatcgcga cccgcgtcag cgcatcggga
     4501 ccgttgccga tcagggtcgc gcccttgacc gggcgggtga tcttgccgtc ttcgatcagg
     4561 taggcttcgg tggtggagaa cacgaacttg ccgttggtga tgtccacctg accgccgccg
     4621 aagttcaccg catacaggcc gtgtttcacc gaggcgatga tctcttcgcg agtcttgtcg
     4681 ccgttgagca tcatggtgtt ggtcatgcgc ggcatcggca ggtgggcata ggattcgcgc
     4741 cgcgcattgc cggtcaccgg cacgcccatc agacgcgcat tcaggctgtc ctgcagatag
     4801 cccttgagga tgccgtcttc gatcagcgtg gtgcactggg tctggttgcc ctcgtcgtcc
     4861 atctgcaagg aaccgcgccg actggcgatg gtgccgtcgt ccaccaccgt cacgcctttg
     4921 gccgcgacgc gttcgccgac gcgaccggag aaggccgaac tgcccttgcg gttgaagtcg
     4981 ccttccaggc cgtggccgat cgcctcatgc aacaggatac cgggccagcc gctgcccagc
     5041 accacggtca tctcgcccgc aggcgcgggg cccgcatcga ggttgatcac ggcctgatgc
     5101 acggcctgct cggcatagcg gtgcagcatc gcgtcgtcga aatagtcgta gtcgaaacgc
     5161 ccgccgccgc ccgccgaacc ctgttcgcgt ttgccgttgg cttcgacgat gacctgtacc
     5221 gacaagcgca ccagcggacg gatgtcggca ttcatcaggc cgtcgctgcg tgcgaccatg
     5281 accacttcgt actcgcccgc cagtcccgcc atcacctggg tgacgcgcgg atcgatcgcg
     5341 cgagcgtagc gttcgatgcg ctccagcagc gcgaccttgt cggcatcctt catgctggcg
     5401 atgggatcgt tgggcagata gatcgcacag cgcgcaccgc gatgcagcat cggcaccttc
     5461 tgcttgccgc cctgacgcgc gatcgcgcgc gtggcctgcg c
//
LOCUS       DASUIV010000250         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_714599, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000250 DASUIV010000000
VERSION     DASUIV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714599"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..742
                     /gene="nth"
                     /locus_tag="VFW59_09060"
     CDS             110..742
                     /gene="nth"
                     /locus_tag="VFW59_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5364409.1"
                     /translation="MNPAKRQQIFARFQAANPHPTTELNYRTPFELLVAVMLSAQATD
                     VSVNHATRQLFPVANTPQAILALGEDALREYIQRIGLYQTKARHVIQTCRLLLERHGG
                     QVPQTREELEALPGVGRKTANVILNTAFGQPTIAVDTHIFRVANRIGLAPGKTVLEVE
                     KKLLKVIPDEFKLDAHHWLILHGRYICQARKPKCGACIIESLCEYKEKVF"
     gene            742..1164
                     /locus_tag="VFW59_09065"
     CDS             742..1164
                     /locus_tag="VFW59_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEX5364410.1"
                     /translation="MFNPTRDEARNFLFESWRKRRAGELLTPLEDLTAQLIDKHPEFH
                     AVLEDPERYQDKDYQPEHGTTNPFLHLMMHLTIEEQISIDQPAGIRAHFARLTRQYES
                     EHEAQHRMMECLGEMIWQAQRNRTQPDALVYLGCLEGR"
     gene            1285..>3119
                     /locus_tag="VFW59_09070"
     CDS             1285..>3119
                     /locus_tag="VFW59_09070"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5364411.1"
                     /translation="MPSKFWLRLLRNYLPVALLLCALGIAFYASESAEDIDGIIRSEH
                     THLRNAALAADRDLLSLARDTQYLANTLPLTKAIDSRHATALEAEAASEFVTYLGAKQ
                     IYSKLRWIDETGREQIHIDRSRNGVTTIAPERDEQDKSRRYYFAETMKKQKGETYLSP
                     LDLEIENGSIELPLHPVLRAATPVFDHAGQRRGLVIVTYDAQELFRDIGNIFESEIGN
                     WMLFDQDGYWLRSPDKADEFGFMLPHRANMAARSPSVWARINAAPQGHFTDESGDLWV
                     FTDIAPYRAIASSAPQDSTPPAMSWKMVKHISTDALAARSKSLRDKTLALVAVLLALS
                     LLVVLRLTRSQLGKERDEAKLRQAASELGQQKFALDQHAIVAITDVNGTITYVNDKFC
                     TITQYPREELLGQNHRMLNSGKHDPEFFQKMLHTIASGEVWHSEVCNRAKDGSLYWVN
                     STFVPLQDESGKPRAFIAISTDVTQTKLHEQRLEEAQRLGKIGHWELDLTSGRLNWSS
                     ETFSIFEIDPQRFSASYEAFLKLVHPEDRELVQQGYNDSVANGTAYDIQHRLLFADGR
                     IKWVSERGVTHYAPDGKALLSVGTVQDITAQKEAEEKLRIASIAF"
BASE COUNT          707 a         1028 c          876 g          508 t
ORIGIN      
        1 gggcgatgcg cccggctgac cggccggacg cgtaccagag cttagcggat ggcgcggctt
       61 ctcctagaat cagcgggccc gttccagacc aatttccgag tcgccgcccg tgaaccccgc
      121 caaacgccag cagatcttcg cccgcttcca ggcggccaac ccccacccca ccaccgagct
      181 gaactaccgc acgccgttcg agctgctggt cgccgtgatg ctgtcggcgc aagccaccga
      241 cgtcagcgtg aaccacgcca cgcggcaact gttcccggtc gccaacaccc cgcaggcgat
      301 actcgcgctc ggcgaagacg cgttgcgcga atacatccag cgcatcggcc tgtaccagac
      361 caaggcccgg catgtgatcc agacctgccg cctgctgctg gagcgccacg gcggccaggt
      421 gccgcagacc cgcgaggaac tggaagccct gcccggcgtg ggccgcaaga ccgccaacgt
      481 gatcctcaac accgccttcg gccaacccac catcgcggtg gacacccata ttttccgcgt
      541 cgccaaccgc atcgggctgg ctcccggcaa gaccgtgctg gaagtcgaaa agaaactgct
      601 caaggtgata cccgacgagt tcaagctcga cgcgcaccat tggctgatcc tgcatggccg
      661 ctatatctgc caggcgcgca aaccgaagtg cggcgcgtgc atcattgaga gcctgtgcga
      721 gtacaaagag aaagttttct gatgttcaac ccgacccgcg acgaagcgcg caacttcctg
      781 ttcgaatcct ggcgcaagcg ccgcgccgga gagctgctca cgccgctgga agacctcacc
      841 gcgcagctca tcgacaagca tcccgagttc catgccgtgc tggaagaccc ggagcgctat
      901 caggacaagg actaccagcc ggaacacggc acgaccaatc cctttctgca cctgatgatg
      961 cacctgacca tagaggagca gatttccatc gaccagccgg cggggatacg cgcgcatttc
     1021 gcccgcctga cccggcagta cgagtccgag cacgaagcgc agcaccgcat gatggaatgc
     1081 ctgggagaga tgatctggca ggcgcagcgc aaccgcaccc agccggatgc gctggtttat
     1141 ctgggctgtc tggaaggccg gtgattctcg attcccgccg ccgagtcgat aaaaaaaccg
     1201 gagccgcacg gcaaccgtcc cgactcgcca cgcctgtgat aatcttcgcc cgctttgcat
     1261 aaaataaatc tgactgacat tttcatgccc tcgaaattct ggctgcggtt gctgcgaaat
     1321 tacctgcctg tagcgctgct cttatgcgcg ctgggcatcg cgttttacgc gagcgaatcc
     1381 gccgaagata tcgacggcat tatccggagc gaacatactc atctccgcaa tgcggcactc
     1441 gcagccgacc gcgacttgct cagtctggcc cgcgatacgc aatacctggc caacaccctt
     1501 ccgctgacaa aagccataga cagccgacac gccactgcgc tggaagcgga ggccgccagc
     1561 gaattcgtca cctatctcgg cgccaaacaa atttacagca agctgcgctg gatcgatgaa
     1621 accggtcggg aacagataca catcgaccgg agcagaaatg gcgtaacaac catcgctccc
     1681 gaacgcgacg aacaagacaa atcccggcgt tactactttg ccgaaacgat gaaaaagcag
     1741 aaaggcgaaa cctacctttc gccgctggat ctggaaatcg aaaacggcag catcgagctg
     1801 ccgctccatc cggtgctgcg cgccgcaacc ccggtattcg atcacgcggg ccaacggcgc
     1861 ggcttggtca tcgttacgta cgacgcgcag gaactatttc gggacatagg aaatattttt
     1921 gaatccgaga tcggcaactg gatgctgttc gatcaggacg gatactggtt gcgcagcccc
     1981 gacaaggcgg acgaattcgg cttcatgttg ccgcaccgcg ccaatatggc ggctcgcagc
     2041 ccgtcggtct gggccagaat caacgccgcc ccccagggac atttcaccga cgaatccggc
     2101 gatttatggg tgtttacgga catcgccccc taccgggcaa tcgcatcttc tgcgccgcag
     2161 gactcaacgc cccctgcgat gtcctggaaa atggtcaagc acatcagcac tgacgcgctg
     2221 gccgcaagga gcaagtcgct gcgcgacaag acgctggcgc tggttgccgt actgctcgca
     2281 ttgagcctgc tggtggtgct gcgcctgacc cgctcacaac tgggcaagga aagggatgag
     2341 gccaagttgc ggcaggcggc aagcgaactc ggccagcaaa aattcgcgct cgaccagcat
     2401 gccatcgtcg cgatcaccga tgtgaacggc acgatcacgt acgtcaacga caagttctgc
     2461 accatcaccc aatacccccg cgaggaattg ctcgggcaaa atcaccgcat gctcaattcg
     2521 gggaaacacg atcccgaatt tttccaaaag atgctgcaca ccatcgcatc gggcgaggtc
     2581 tggcacagcg aagtctgcaa tcgcgccaag gacggcagcc tgtactgggt caacagcacc
     2641 ttcgtgccgc tgcaggatga atcgggcaaa ccccgcgcct tcatcgcgat cagcaccgac
     2701 gtcacccaga ccaagctgca cgagcagcgc ctcgaagaag cgcaacggct gggcaagatc
     2761 ggccattggg aactggacct gacgagcgga cgcctgaact ggtcgagcga aacgttcagc
     2821 atcttcgaga tcgacccgca acgcttcagc gcgtcctacg aggcgttcct gaaactggtt
     2881 cacccggaag accgggaact ggtccagcaa ggttataacg actcggtcgc caacggcact
     2941 gcctacgaca tccagcaccg cctgctgttc gccgacggac gcatcaaatg ggtcagcgag
     3001 cgcggcgtca cgcactatgc gccggacggc aaggcgctgc tgtcggtggg cacggtgcag
     3061 gacatcaccg cgcagaagga agccgaagaa aaactgcgca tcgcctccat cgccttcga
//
LOCUS       DASUIV010000251         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_156304, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000251 DASUIV010000000
VERSION     DASUIV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156304"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="VFW59_09075"
     CDS             complement(<1..2067)
                     /locus_tag="VFW59_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5364412.1"
                     /translation="MMLRKFFDNCPVSEPADVLQLEQTKLLYANLPAAIAINLLLALL
                     LAGVESAVVSPYALFVWLSVVGMTLLLRIALLLAWRRSEIDAGNGGCWLRRFRIAVVA
                     TGAAWGMGALLLFPVGDIDHQSYLAFAMAGLSAGAISALAVDRVSTLGFLIPMLLPLV
                     VRLWLEGGKLALSMGMMVMLFLIFIAMNASRGRRSLHENFQLRIKAEEQEQSLRQKEA
                     SLREAQQTAHVGNWELDLVGNKLSWSDEIYRIFEIDPATFDPSYEAFLNAVHPDDRAA
                     VNLAYTRSLETREPYEISHRLLMGDGRIKWVIEKCNSFFDAQGKPVRSVGTVQDATRE
                     KEFEAILQESERRYRFLFSNNPLPMWVFAEDDFRFLEVNERAIQHYGYSREEFGRMSL
                     RDIRPEEDIALLNQVISSSPSGVVTIEARHQKKDGTSINVRVSTMPMVFAGVPARIAL
                     VQDITEQKQAEQEREWQIRNQQALLDAIQETTFLMERNGTMLVVNGVGAQRLNATPEE
                     LVGKNIYEILPPDLAETRRAHFERIARTGKSETMEDERAGRRLLSSIYPVMDDNGGVS
                     RFAVYAADVTQQRRVEAIEALFSAINQRVLQGQPIAELLQFICGEVARLFNLDLIWLG
                     RKEADGAVSIMTQAGAAIGYVEGVKRLGVRWDDTPQGRGATGTAIRSGQIQVINASNP
                     GFRLWKE"
     gene            complement(2375..2803)
                     /locus_tag="VFW59_09080"
                     /pseudo
     CDS             complement(2375..2803)
                     /locus_tag="VFW59_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
     gene            2803..3098
                     /locus_tag="VFW59_09085"
                     /pseudo
     CDS             2803..3098
                     /locus_tag="VFW59_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293285.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
BASE COUNT          637 a          994 c          862 g          605 t
ORIGIN      
        1 ctccttccac aacctgaaac ccggattgga cgcgttgatg acctgtatct ggccgctgcg
       61 tatcgccgtt ccggtggcgc ctcgtccctg cggcgtgtcg tcccagcgca caccgagacg
      121 cttcacgccc tctacgtagc ctatcgccgc ccccgcctgc gtcatgatgc tgaccgcccc
      181 atcggcctcc ttgcgcccca gccagatcag gtccagattg aacagtctgg ccacctcgcc
      241 gcagatgaac tgcagcaact ccgcgatggg ctgtccctgc aacaccctct ggttgatcgc
      301 ggagaacagc gcctcgatcg cctcgacccg ccgctgctgg gtgacatccg ccgcataaac
      361 cgcaaaacga ctcaccccgc cgttgtcgtc cataaccgga tagatggaac tcaacaaacg
      421 ccggccggca cgttcgtcct ccatggtttc cgacttgccg gttcgcgcga tccgctcgaa
      481 gtgcgccctg cgcgtttcgg ccagatcggg agggaggatt tcatagatat tcttgccgac
      541 caactcctct ggcgtggcat tcaaacgctg cgcccccact ccgttgacca ccagcatggt
      601 gccgttccgt tccatcagga aggtagtctc ctggatggca tccagcaagg cttgctgatt
      661 gcggatctgc cattcgcgct cttgttcggc ctgcttctgc tcggtgatat cctgcaccag
      721 cgcgatacgg gcgggaacgc ccgcaaacac catcggcatg gtgctgaccc gcacattgat
      781 cgacgtgccg tccttctttt ggtgccgagc ttcgatggta acgaccccgc tgggtgaact
      841 ggagatcacc tgattgagca gtgcaatatc ctcctcaggc cggatatccc gcagcgacat
      901 ccggccgaat tcctcgcggc tgtatccgta gtgttgaatc gcccgctcgt tcacctcgag
      961 aaacctgaag tcatcctccg cgaagaccca catgggcagt ggattgttgc tgaacagaaa
     1021 gcgatagcgt cgctcgctct cctgcaagat cgcctcgaat tctttttctc tggtcgcatc
     1081 ctgcaccgtg ccaaccgacc gcaccggctt accctgcgca tcaaaaaagg aattgcattt
     1141 ctcgatcacc catttgatgc gcccgtcgcc catcaacaga cgatggctga tctcataggg
     1201 ctcgcgcgtc tccagcgaac gggtataggc cagattcacc gctgccctgt catccgggtg
     1261 aaccgcgttg agaaaagcct cgtaggaggg atcgaaggta gcagggtcga tttcgaagat
     1321 gcgatagatt tcatcggacc aggacaactt gttgccgacc agatccagtt cccagttgcc
     1381 gacatgggca gtctgttgcg cctctctcag gctcgcctct ttctggcgca agctctgctc
     1441 ctgctcctca gccttgatgc gcaactgaaa attctcgtgc aggctgcgtc ggccacgcga
     1501 ggcattcatc gcgataaaga tcaggaacag catcaccatc atgcccatgg acaaggccag
     1561 ttttccgcct tccagccaaa gacgcacgac caggggcagc agcatcggta tcaaaaaacc
     1621 cagcgtggac acgcgatcga ccgccagcgc cgaaatggcg ccggcgctca gcccggccat
     1681 cgcgaaggcc agataggact gatggtcgat gtcgccgacc ggaaacagca gcaatgcgcc
     1741 catgccccac gccgcgccgg tcgcaacgac cgcgatgcgg aaacgacgta gccagcagcc
     1801 accgtttccg gcgtcgatct cgctacggcg ccaggccagc agcagcgcga ttctgagcag
     1861 cagggtcatc cccaccacgg agagccagac gaacagcgca taggggctga ctaccgcgct
     1921 ttcaacgcct gccagcagca gcgccagcag caggttgatg gcgatcgccg ccggcaggtt
     1981 ggcatacagc agcttggtct gttcaagctg cagcacatcg gccggctcgc tgacggggca
     2041 gttgtcaaaa aatttacgca gcatcatgca tcactccccg attgatcatc ggattcaaaa
     2101 ccggattcac cgcaccgacc gccacgctcg gaatcgcgca aactcgagag ccagtggcgc
     2161 gaatgggttg gggttatcgc tcatccatac ccggtttgtt tttatgggaa ccacatctga
     2221 aattgtactc agcatcagac agtgcgatgt aaatatttac acggaacagt cgaaatatcc
     2281 gccggacagc cactgcgcca gacacaataa aatgcaaaaa aaaacggaag ccgctcgggc
     2341 ttccgttcaa tcggctcgta gcgcagcgga tcgcctaggc tttcagcgcg gccagcactt
     2401 cttccagcat tttcttggca tcgccgaaca acatgcggtt gttgtccttg tagaacagcg
     2461 gattatccac gcccgcatag ccggtcgcca tgctgcgctt catcatgatc gaggtcttgg
     2521 ccttccagac ttccagcacc ggcatgccgg cgatggggct gcccggatct tcctgagctg
     2581 ccggattcac gatgtcgttg gcaccgatca cgatggcgac atcggtatcc ggaaagtccc
     2641 cattgagttc atccatctcc atcacgatgt cgtaaggcac tcgggcttcg gccagcagca
     2701 cgttcatgtg accgggcatg cggccggcga ccgggtggat cgcgaagcgg acgtcgacgc
     2761 cgcgcttggt cagctgatcg tagagctcgc ggacgcggtg ctgcggaacc ggcgggcgaa
     2821 gtgatctcta tcagcgcgac cgagacggca gagctgctgc gcgatgcgaa gagcgtgatc
     2881 atcgtgccgg gctacggcat ggcggtggct caggcgcagc acacggtcaa cgagatcacg
     2941 cagttcttgc gcgagaaagg cgtcaacgtg cgcttcggca tccacccggt tgccggccgc
     3001 atgcccggtc acatgaacgt gctgctggcc gaagcacgag tgccttacga catcgtgatg
     3061 gagatggatg aactcaatgg ggacttcccg gataccga
//
LOCUS       DASUIV010000252         4602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1364290, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000252 DASUIV010000000
VERSION     DASUIV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364290"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFW59_09090"
     CDS             <1..627
                     /locus_tag="VFW59_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX5364413.1"
                     /translation="VALSAGAGNKVLTVPTSAVIDSGTRQVVLVQLAQGRFEPRAVRL
                     GVRSDDYVEVREGVAEGEQVVTSANFLIDAESNLKAVLGGLSVHAAHGGPAAEPQPAV
                     AEHTGHAAPAQPAASAQAVKKPATVGHRAQGILNVVNADGTVSITHEPIATLGWPGMT
                     MDFALANAALAQGIAPGSAISFELVERKPDEWVITKLQVRARHQHEGH"
     gene            629..3847
                     /locus_tag="VFW59_09095"
     CDS             629..3847
                     /locus_tag="VFW59_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5364414.1"
                     /translation="MLNTIIEWSARNRFLVLLATLFIALAGSYAVLKTPLDALPDLSD
                     VQVIVYTEYPGQAPQVVEDQVTYPLTTAMLAVPRSKVVRGFSFFGASFVYVIFEDGTD
                     IYWARSRVLEYLNSIAGHLPKNVAPQLGPDATGVGWVYQYAVLSEKHNLAELRTMQDW
                     YLRYQLTKAHGVAEVASIGGFVQQYQVTVDPVKLRAYGIPLSKVSQVIRESNRDVGGR
                     VVEMAETEYMVRGRGYLRGKSDIENLVVKAERGTPVLLRDIARVELGPDERRGIAELN
                     GDGEVVSGIAMARYGQNALEVIRNIKDKIAEITPGLPEGVSIQTVYDRSELIRRAIAT
                     LKKTLFEEGLIVALVCVVFLMHARSAMVAIVMLPIGVLIAFIAMRLLGMNSNIMSLGG
                     IAIAIGAMVDAAIVMIENAHKHLERLENNPNPPQPSLIREGVMPPPLTRGGREGFGFD
                     QRAAAILSACQEVGPSLFFSLLIITVSFLPVFTLEAQEGRLFAPLAFTKTFAMAGAAL
                     LSVTLVPVLMMLFIRGHIMPESQNPLNRWLIRSYRPVITAVMRRKKTTIAVAALALIL
                     SLYPAMRLGSEFMPTLNEGTLFYMPASLPGMSVTKAAELLQTQNKIIKSFPEVASVYG
                     KAGRAQTATDPAPLEMFETVINLKPQEEWRPGMTTDKLIAELDRALQFPGVANSWTMP
                     IKARIDMLSTGIRTPVGIKVFGKNLDEMERLAKEIEAVVRKVPGASSAFAERITGGFY
                     LNIEPDRQVLARYGLTVGEVQDVIATALGGETVTTTVEGLERFGVNVRYPRELRGTPE
                     QIAREVLVPTMDGAMIPLGQLARVSISKGAPSIRTENALLSAYIYVDIRDRDIGSYVA
                     DARKAVAAQVKFPPGYYATWSGQFEYMERATAKMKIVVPITLLLIFLLLYLNFRRVTE
                     SLIVMLSVPFALVGGVWLLWLLGYNLSVAVAVGFIALAGVAAETGVVMLIYLENAWQE
                     RQAACAAEGRTPVLADLHGAIMEGAVERVRPKMMTVTAIMAGLLPIMWGTGTGSEVMS
                     RIAAPMIGGMISSTVLTLAVIPALYALAKQREITRTISNSKN"
     gene            3859..4206
                     /locus_tag="VFW59_09100"
     CDS             3859..4206
                     /locus_tag="VFW59_09100"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX5364415.1"
                     /translation="MKTLTLAFGITLIASNMAHADAYEHDMSQHRKTMQTASREVKHE
                     GSGVLKAVNLEAGKVQIAHQPIEALGWPAMTMWFALRSPLPQDTKVGDSVRFELEQTQ
                     SKDWVITRIERKR"
     gene            4397..>4602
                     /locus_tag="VFW59_09105"
     CDS             4397..>4602
                     /locus_tag="VFW59_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364416.1"
                     /translation="MKYVPSLPPVVKGGGEELDVHAMGRVKPVKAVALQSLSPIVGQP
                     RVRSENYPFIEEPLERRHAPQALR"
BASE COUNT          888 a         1324 c         1547 g          843 t
ORIGIN      
        1 gtcgcgcttt cggcgggggc gggtaacaag gtactcaccg tgccgacttc cgccgtgatc
       61 gacagcggca cgcgccaggt cgtgctggtg caactggcgc agggaagatt cgagccgcgt
      121 gccgtcaggc tgggtgttcg cagcgatgat tacgtcgagg tgcgggaagg tgtcgcggaa
      181 ggcgagcagg tggtgacttc cgccaacttc ctgatcgacg cggagagcaa tctgaaggct
      241 gtgctgggcg ggctgagtgt tcatgcggcg catggcggcc cggctgccga gccgcaaccg
      301 gcggtggcag aacatacagg gcacgccgca cctgcgcagc ccgccgcatc tgctcaggcc
      361 gtaaagaaac ccgctacggt cggacatcgg gcgcagggca ttctgaatgt ggtcaatgca
      421 gacggcacgg tcagcatcac gcatgagccg attgcgacgc tgggctggcc gggcatgacg
      481 atggattttg cgctggccaa tgctgcgctg gcgcaaggca tcgcgccggg cagcgcgatc
      541 agcttcgagc ttgtcgaacg caaacctgac gaatgggtga tcaccaagct gcaagttcgc
      601 gcgcggcatc aacacgaggg ccactgacat gctgaacacg attatcgagt ggtcggcacg
      661 caaccgtttc ctggtgctgc tggccacgct gttcatcgcg ctggcgggca gctacgcggt
      721 gctcaagacg ccgctggatg cgctgccgga tttgtcggac gtgcaggtga tcgtctatac
      781 cgagtatccc ggacaagcgc cgcaggtggt ggaggatcag gtcacctatc cgctgaccac
      841 cgcgatgctg gcggtgccga gatccaaggt ggtacgcggc ttctcgtttt tcggcgcatc
      901 gttcgtgtac gtgattttcg aggacggcac cgacatctac tgggcgcgtt cgcgcgtgct
      961 ggaatacctc aacagcatcg cgggacactt gcccaaaaat gtcgcgccgc aactcgggcc
     1021 ggacgcgacc ggcgtgggtt gggtgtacca gtacgcggtg ctcagcgaga agcacaactt
     1081 ggccgagctg cgtaccatgc aggactggta tctgcgctat cagctcacca aggcgcacgg
     1141 cgttgcggag gtcgcctcca tcggcggatt tgtgcagcag tatcaggtga cggtcgatcc
     1201 agtgaagctg cgcgcctacg gtatcccgct gagcaaagtc tcgcaggtga tccgcgagtc
     1261 gaaccgcgac gtcggcggcc gcgtggtcga gatggccgag accgagtaca tggtgcgggg
     1321 acgcggttac ctgcgcggca agagcgacat cgagaatctg gtggtgaagg ccgagcgcgg
     1381 cacgccggtg ctgctgcgcg acatcgcacg cgtcgaactg gggccggacg aacggcgcgg
     1441 catcgcggaa ctcaacggcg acggcgaagt ggtgtccggc atcgctatgg cgcgctacgg
     1501 gcagaacgcg ctggaggtga tccggaacat caaggacaag atcgccgaga tcacgcccgg
     1561 tctgccggaa ggcgtgagca tccagacggt atatgaccgc tccgaactga tccggcgcgc
     1621 gatcgcgaca ctcaaaaaaa ctttgttcga ggaaggtctg atcgtcgcgc tggtctgcgt
     1681 cgtgttcctg atgcacgcgc gcagcgcgat ggtcgccatc gtgatgctgc ccataggcgt
     1741 gctgatcgcg ttcatcgcga tgcgtctact tggcatgaac tccaacatca tgagcttggg
     1801 cgggatagcg attgctatcg gcgcgatggt ggatgcggcg atcgtgatga tagagaacgc
     1861 gcataagcat ctggagcggc tggaaaacaa ccccaaccct ccccaaccct cccttatcag
     1921 ggagggagtc atgcctcctc ccctgacaag gggaggccgg gaggggtttg gttttgacca
     1981 gcgagccgcc gccatcctct ccgcctgcca ggaagtcggc ccttcgctgt ttttctcgct
     2041 gctgatcatc accgtgtcgt tcctgccggt gttcacgctg gaagcgcagg agggcaggct
     2101 gttcgcgccg ctggccttca ccaagacctt cgcgatggcc ggtgcggcgt tgctgtcggt
     2161 cacgctggtg ccggtgctga tgatgctgtt catacgcggc cacatcatgc cggagtcgca
     2221 gaatccgctg aaccgctggc tgatccgcag ctaccggccg gtcatcaccg ccgtgatgcg
     2281 ccgcaaaaag accaccatcg ccgtcgccgc gctggccttg atattgagct tgtatcctgc
     2341 catgcgcttg ggcagcgagt tcatgccgac gctaaacgag ggcacgctgt tctacatgcc
     2401 tgcctcgctg ccgggcatgt cagtgaccaa ggcggccgag ttgttgcaaa cgcagaacaa
     2461 gatcatcaag agcttccccg aagtcgcgtc ggtgtacggc aaggcggggc gcgcgcagac
     2521 cgccaccgac ccggcgccgc tggagatgtt cgagacggtg atcaacctca agccgcagga
     2581 ggaatggcgg cccggcatga ccaccgacaa gctgatcgcc gagctggacc gcgcgctgca
     2641 attccccggt gtcgcgaact cgtggaccat gccgatcaag gcgcgcatcg acatgctctc
     2701 taccggcatc cgcacgcccg tcggcatcaa ggtgttcggc aagaacctgg acgagatgga
     2761 gcgccttgcc aaggaaatcg aagccgtggt caggaaggtt ccgggtgcca gcagcgcgtt
     2821 tgccgagcgc atcaccggcg gtttctatct gaacatcgag ccggaccggc aggtgttggc
     2881 gcgctacggg ctgacggtcg gcgaggtgca ggatgtgatc gccaccgcgc tgggcggcga
     2941 gactgtcacg acgactgtcg aagggctgga gcgcttcggc gtgaacgtgc gttatccgcg
     3001 cgagttgcgc ggcacgcctg agcagatcgc gcgcgaggtg ctcgtcccca cgatggacgg
     3061 agcgatgatc ccgctcggcc aattagcccg cgtcagcatc agcaagggcg cgccgtcgat
     3121 ccgcaccgag aacgcgctgc tgtccgccta catctatgtg gacatccgcg accgcgacat
     3181 cggctcgtat gtcgccgatg cgcgcaaggc tgtggcggcg caggtgaagt tcccgcccgg
     3241 ctactacgcg acctggagcg ggcagttcga gtacatggaa cgtgcgactg cgaagatgaa
     3301 aatcgtcgtg ccgatcacat tgctgctgat cttcctgctg ctgtatctca acttcaggcg
     3361 tgtcaccgag tcgctgatcg tgatgctgtc cgtgccgttc gcgctggtcg gcggcgtgtg
     3421 gctgctgtgg ctgctcggct acaacctgag cgtggcggtg gcagtcggct tcatcgcgct
     3481 ggccggcgtt gcggcggaga ccggcgtggt gatgctgatc tatctggaaa acgcctggca
     3541 ggaacggcag gccgcctgcg cagcggaagg gcgcacgccg gtgctggccg acctgcatgg
     3601 cgccatcatg gaaggcgcgg tggagcgggt gcggccgaag atgatgacgg tgacggcgat
     3661 catggccggg ctgctgccga tcatgtgggg taccggcacc ggctcggagg tgatgagccg
     3721 catcgccgcg ccgatgatag gcgggatgat ctcgtctacg gtgctgacgc tggcggtgat
     3781 tcccgcgtta tatgcgctgg ccaaacagcg cgaaattacg cgaacgattt caaacagtaa
     3841 aaactgaaag gaatagcaat gaaaacactg actctggcat tcggcataac gctcatcgca
     3901 agcaatatgg cacatgccga tgcttatgaa cacgacatgt ctcagcatcg gaagacgatg
     3961 caaaccgcaa gccgggaagt taaacacgaa ggcagcgggg tgctgaaagc ggtgaacctg
     4021 gaagcgggca aggtgcagat cgcgcaccaa cccatagagg cgttgggctg gccggcgatg
     4081 accatgtggt tcgcgctgcg ttcgcccttg ccgcaagata ccaaggtcgg tgatagtgtg
     4141 cgcttcgaac tggaacaaac ccagtctaag gattgggtaa ttacccgcat tgagcgaaag
     4201 cgctgaataa ttcgaggggg tgcaatgtcg gtgatagcaa tgcgctccgg cctggtcgga
     4261 taaggttgtg gtcggtatga actttcttgc aaattgcatc gccgcatgtg agcaagcaaa
     4321 atgagcccgg tcatcatttg aaaagtcggg ggctacccct gaccagtgcg ttatcctcag
     4381 ttcgggaggt gaaatcatga aatatgtgcc tagtctaccc ccggtggtaa agggtggggg
     4441 agaggaattg gatgtgcatg ccatgggccg ggttaagccg gtcaaggctg tagcgctgca
     4501 aagcctgtca ccgattgtgg ggcagccgcg cgtccggtct gaaaactacc cttttatcga
     4561 ggagccgctg gagcggcggc atgcgccgca agccttgcgg ga
//
LOCUS       DASUIV010000253        12222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_64685, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000253 DASUIV010000000
VERSION     DASUIV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 12222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12222
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64685"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..531
                     /locus_tag="VFW59_09110"
     CDS             277..531
                     /locus_tag="VFW59_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX5364417.1"
                     /translation="MAHVILAETTASVSELKKNPMATVAAGEGFPVAILNRNEPAFYC
                     VPAKAYEALMDKLEDMELNALADARLKDGKKLVPVKLDDL"
     gene            521..802
                     /locus_tag="VFW59_09115"
     CDS             521..802
                     /locus_tag="VFW59_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5364418.1"
                     /translation="MTYKLSFHPDALAEWRKLDATVSEQFKKKLAERLPHPRVPSAKL
                     AGHQDRYKIKLRSVGFRLVYEVRDGVLLVIVVAVGKRERNAVYRVAAKR"
     gene            871..3168
                     /locus_tag="VFW59_09120"
     CDS             871..3168
                     /locus_tag="VFW59_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5364419.1"
                     /translation="MNNLDHKTAIQSAQLQQLLSVSNVSLITSVLLATILAFMQREVI
                     AAPEFFSWIVLMVLASLLRAATVIKYQRYPSDDAAEIQRRLVVFRFVVLIAGLVWSAA
                     AFLLFPAGGTFHQMQHQSFLIFMMMGLTAGGVVSFSSDLRCALFFIVPIIAAVMLRLF
                     LVGDNLSVAMGVAALLYLGFLIMALRRINGHILENITLRIKASAQEEALRASKERYRS
                     LLGHLPVGILHYDRDLVMTYCNERLAEFLHSSIDRLVGLDMKLVKDQSILPALKLALD
                     GERGYYEGHYRATYTDADGWMEMTCTPYRDDAGTVVGGVAIVHDVSERKQAEERIHQL
                     AFYDPLTLLPNRRLLMDRLQHAVAVGARSDASHALLFIDLDNFKVLNDTRGHDVGDRL
                     LVEAARRLRSCVREGDTVARLGGDEFVVILEDLSVTEEQAVVQAEIAGEKIRATLNQP
                     YRLGAIDHHCSCSIGISLFHDHQVSVDELLKRADTAMYEAKTAGRNALRFFDPAMQSA
                     LEARAALENELRQALPLRQFRLYYQKQVNVDGDPVGAEALLRWIHPERGLVPPMEFIP
                     LAEESELILSIGLWVLETACAQIKAWESHPLSRELQLAVNVSARQFHQADFVDQVRAV
                     VDRFRINPDRLKLELTESVVLDDINDTIAKMRELKRIGVRFSMDDFGTGYSSLAYLTQ
                     LPLDQLKIDRSFVSNIGIKSTDAVIVQSIINMGQNLGMDVIAEGVETEAQRDFLMRQG
                     CDAYQGFLFGRPQPLDEFDRQLGSS"
     gene            3362..3787
                     /gene="ndk"
                     /locus_tag="VFW59_09125"
     CDS             3362..3787
                     /gene="ndk"
                     /locus_tag="VFW59_09125"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5364420.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGQITARFENAGLKIIAARMVQLSR
                     AEAEGFYAVHAARPFFKDLVDFMISGPVMVQVLEGEGAILKNRDLMGATDPKKAEKGT
                     IRADFADSIDANAVHGSDAAETAAVEIAYFFPALNVYSR"
     gene            3803..4885
                     /gene="rlmN"
                     /locus_tag="VFW59_09130"
     CDS             3803..4885
                     /gene="rlmN"
                     /locus_tag="VFW59_09130"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5364421.1"
                     /translation="MKQNLLDFDVQQLTAWFAANGEKPFRAKQVLRWIYKAGESDFDA
                     MSDLAVSLREKLKQIACVQAPKVMREELSDDGTRKWLLDMGTGNAVETVYIPEETRGT
                     LCVSSQAGCALDCAFCSTGKQGFNRNLSVAEIVGQLWWANHQLGKDEDGNWRISNVVM
                     MGMGEPLLNFDNTVSALRLMLDDHGYGLSRRRVTVSTSGVVPAMDRLREECPVALAVS
                     LHAPNDALRDVLVPINRKYPLNELMAACRRYLEKAPRDFITFEYVLLDGVNDSEQQAR
                     ELVKLVRDVPCKFNLIPFNPFPQTQYQRSKPEAIRRFRDVLMQADIVTTTRKTRGEDI
                     AAACGQLAGQVQDKTKRTMQRITEVH"
     gene            4885..5640
                     /gene="pilW"
                     /locus_tag="VFW59_09135"
     CDS             4885..5640
                     /gene="pilW"
                     /locus_tag="VFW59_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEX5364422.1"
                     /translation="MKRLFILSLLLLSACGTPSGGGSQPQSNSRGTASAKIHTELAGM
                     YYERSQLGIALAEIEAALQADRNYAPAYQVRGLIHMTLREDKEAEEDFKQSLHLDKDD
                     SDTHNNYGWFLCQRDRAKESIHHFMAALKNPLYTTPERAYLNAGVCSQKAGNLKDASE
                     FLQRALLLQPGSPQALIALAELNFAKGDYSVAKKYFAEFAQRIESPSAEQLWLGVRIE
                     RKVGDRNAAANYAVQLRKRFPDAQETQWLMQGE"
     gene            5664..6701
                     /locus_tag="VFW59_09140"
     CDS             5664..6701
                     /locus_tag="VFW59_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX5364423.1"
                     /translation="MNTNEGAAMPVLPGAGLREARERLGLSVADAADQTKLAPRQIEA
                     LEADDFAHLPEMAFVRGFVRSYAKILQLDPQPLLAMLPQAIASGAAQVTRSSVEVPFP
                     NAHSPRVQNLVWLGAALFLSVLVVVFAVWHYTSPRSQPEPEKIRPVEMAIPLPVEAHV
                     VAASEVAEASAVAEPEAIEPTPPVNPVTPAPVQPVTLAKPVAAQPAAIAVVKPVLPVA
                     ASAVKATQSAAAATKPAVAAPAAKPLSPVAPPAAKPLPAVVPSASSAPTVLRLTFGEE
                     SWAEVRDAKGNIVSSQINPAGSELNLKGQGPFSLVVGHASTSRLFHKGKQIDLTSHAS
                     ANSDVARLTLE"
     gene            6704..7948
                     /gene="ispG"
                     /locus_tag="VFW59_09145"
                     /gene_synonym="gcpE"
     CDS             6704..7948
                     /gene="ispG"
                     /locus_tag="VFW59_09145"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX5364424.1"
                     /translation="MTAVLIKRRPSQRVQVGRVALGGDAPIVVQSMTNTDTADIGATV
                     KQVYELAQAGSELVRITVNTPEAAAAVPAIRKRLDALGCYVPLIGDFHYNGHRLLTDY
                     PECAQLLAKYRINPGNVGREREEADPFTIMIETAIRYEKPVRIGVNWGSLDQRLVVRM
                     MDEKSRLTEPKDVKEVTRAALIASALESAQRAEQLGMPHDRIVLSCKVSEVQDLIAVY
                     RALARQCDYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRVSLTPAPGGSRTE
                     EVIVAQEILQTMGLRAFAPMVTACPGCGRTTSTVFQELAQSIQAHLRTQMPLWRRQYD
                     GVENMTVAVMGCVVNGPGESKLANIGISLPGTGETPAAPVYIDGEKAMTLRGDNIAAE
                     FTRIVDEYVERKYVKKEMESSE"
     gene            8015..9289
                     /gene="hisS"
                     /locus_tag="VFW59_09150"
     CDS             8015..9289
                     /gene="hisS"
                     /locus_tag="VFW59_09150"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5364425.1"
                     /translation="MSKETLQAVKGMNDILPEEAELWLWFEEVVREWLHSYGYRNIRM
                     PLVEPTALFKRAIGEVTDIVEKEMYSFEDALNGDHLTLRPEGTASCVRAVLQHNLLYN
                     APQRLYYSGQMFRHERPQKGRYRQFHQVGVEALGFAGPDMDAEQIIMCARLWRRLGIR
                     DVTLQLNTLGDSASRHRHREKLITYFEQHESLLDADATRRLHSNPLRILDSKNPAMQE
                     LIAGAPQLMDELEQDAIDHFEAVQAILKRHDIAFEINPRLVRGLDYYNRTVFEWVTTR
                     LGAQGTICAGGRYDGLIEQIGGKPAPAMGFAMGVERLLALVLEDGMTPPKPEVDVYVV
                     HQGDQAARLASQVAEQLRDDKLRVLLHCGGGSFKSQMKKADASGARIAVVIGDDEALA
                     NQVSVKPMQGGEQLRVGVGQLIETINNIIKTR"
     gene            9293..9931
                     /locus_tag="VFW59_09155"
     CDS             9293..9931
                     /locus_tag="VFW59_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5364426.1"
                     /translation="MAALDLQEQEQIDALKAWWKDNGKYVLGAVLLLVIAFGGWRGWQ
                     SHQKNKNVEAATLYAEFTRQLESNDAKRVNDAAAAVMGKYADSAYAAHAALLAAQFNE
                     FKKDVAHAKTQLQWVIDHADEASLKDVARLRLAAILLDEKSYDAAMKLLNAAHPDSFN
                     GLYSDLKGDVLNAQGKTDEARTAYKLAFDKTDAKSMYRNLIQMKMDALGVAK"
     gene            9928..11088
                     /gene="bamB"
                     /locus_tag="VFW59_09160"
     CDS             9928..11088
                     /gene="bamB"
                     /locus_tag="VFW59_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEX5364427.1"
                     /translation="MISRPVRLGSGALLLTALFALSGCETLQGWIGKKDPLVEPAKLE
                     DFAASAKFEVRWHTDIGSAKENLLQPAVTRDAIYGASGEGRLTRLDRASGKQVWRIES
                     GIRVTGGVGAGEGLVLIGSDKGEVLAFDENGKPRWKSQVSSEVLSVPQVSDGVVIVRS
                     GDGRIAGLNAADGKRIWLYERSTPTLVVRSHAGVTIQNGVAFAGFAGGKLLALKVKNG
                     DALWESAVSLPRGNTELERISDITSDPVADDQQVCAIAFQGRVACYDVAQGNSLWSRD
                     ISSDKGLMLMRKYLYVTDAAGTVMALDKISGSTLWKNEQLFMRDTAAPYALGDSVVVG
                     DYEGYLHGLSREDGHFTARIKLGSKIQAAPQPLDDGLLVQTRDGDLYSLSLH"
     gene            11102..>12222
                     /gene="der"
                     /locus_tag="VFW59_09165"
     CDS             11102..>12222
                     /gene="der"
                     /locus_tag="VFW59_09165"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5364428.1"
                     /translation="MLPTVVLVGRPNVGKSTLFNRLTRSRDALVADQPGLTRDRHYGR
                     GRVGTRPYLVVDTGGLEPVAKDGILYEMARQSRQAVDEADVVLFIVDGRAGCTPQDMI
                     IAEQLRKTGKPILLLVNKAEGMQRARVTIDFFELGLGEPFPISSAHGDNVEEVVELAL
                     ENFPAEVEPEEKEEQHPKIAIVGRPNVGKSTLVNAILGEQRVIAFDQPGTTRDSIYID
                     FERDGKQYTIIDTAGVRRRGKVDEAIEKFSVIKTMQAVEDANVVVLVVDGADQITEQD
                     AHVADFVLQAGRALVLAVNKWDGLDAHQRETVKSDIERKLHFLSFAKLHFISALNGSG
                     VDKVLKSVEEAYAAAMIKLPTPKLTRALLTALEKQQPPKG"
BASE COUNT         2412 a         3379 c         4050 g         2381 t
ORIGIN      
        1 ggtcagggga tgtcggcgag gactgtctga gcgcgtagcg cgagttccgc agccgcctgg
       61 ccgtttgagc aacgcaggga accccgaagg ggcggcaaac cggggtcgcc ttctttttgg
      121 ttacttttac ttggcgacgc aagaaaaagt gactagctgt cgggctaccc ccgacggttt
      181 tgtttgtatt ttgaatattt caaactatct ccggagacca ttgccgagac ctgttcaggg
      241 tgctatcttg agccccattg aatggaggtt atgcaaatgg cacatgtgat cctggcggaa
      301 acgacggcga gtgtttccga gctgaaaaag aatccgatgg cgacggtggc ggcaggggag
      361 gggtttccgg tggcaatcct gaatcgcaac gagccggctt tctattgcgt tccggcgaag
      421 gcctatgaag ccttgatgga taagctggag gacatggagc tgaatgccct tgccgatgcg
      481 cgcctgaagg atggcaagaa gctggtgccg gtcaagctcg atgacctata agctcagctt
      541 tcatcccgat gcgctggcgg agtggcgcaa gctggatgcg acggtgagcg agcagttcaa
      601 gaagaagctg gccgaacgtt tgccgcaccc gcgagtcccg tcggccaaac tggcggggca
      661 tcaggatcgt tacaagatca agctgcgcag cgtgggtttt cgtctggtgt acgaagtgcg
      721 ggatggcgtg ctgctggtga tcgtggttgc cgtcggcaaa cgcgagcgca atgcggttta
      781 tcgtgtggct gcaaaacgtt gacactgaag tatgattcca gccgggtgct tcgcacataa
      841 gcatattgaa actcactgta gccgcagacg atgaataatc tggatcataa aacggcaatc
      901 caatcggcgc agttgcagca gctgctctcg gtgagcaatg tgtcgttgat caccagcgtg
      961 ctgctggcga cgatactcgc gttcatgcag cgggaggtga ttgccgcgcc ggaattcttc
     1021 agctggatcg tcctgatggt gctggcttcg ttgttgcggg ccgcaaccgt tatcaagtat
     1081 cagcgctatc catcggatga cgctgccgag attcagcgcc gactggttgt ctttcgcttc
     1141 gtggtattga tcgccggatt ggtgtggagt gcggccgctt ttctgctgtt ccccgcaggc
     1201 ggcacattcc atcagatgca gcatcagagt tttttgatct tcatgatgat ggggctgacg
     1261 gcaggcggcg tggtttcgtt ctcgtccgat ctgcgttgcg cgcttttctt catcgtgccg
     1321 atcatcgcgg cagtcatgct gagactgttt ctggtgggcg ataacctgtc cgtggcgatg
     1381 ggcgtggccg cgttgttgta tctcggcttc ctgatcatgg cgctgcgccg tatcaacggg
     1441 catatcttgg agaacatcac cttgcgtatc aaggcctcgg cgcaggaaga ggctttgcgg
     1501 gccagcaagg agcgttatcg ttccttgctg gggcatttgc cggtcggcat tctgcattac
     1561 gaccgggatc tggtgatgac ctattgcaat gagcgccttg ccgaattttt gcatagcagc
     1621 atcgatcgcc tcgtcggtct ggatatgaag ctcgtcaagg atcagtctat tctgcccgca
     1681 ttgaaattgg cgctggatgg ggagcggggc tattacgagg ggcattaccg ggctacctat
     1741 accgatgcgg acgggtggat ggagatgacc tgcacgcctt atcgcgacga tgcaggcacg
     1801 gtcgtgggcg gcgtcgcgat cgttcacgat gtctccgagc gcaagcaggc ggaagagcgc
     1861 atacatcaac tggccttcta tgatccgctg accctgctgc ccaaccgccg cttgctgatg
     1921 gacaggctgc agcatgcggt tgccgttggc gcacgcagcg acgccagtca tgctctgctg
     1981 ttcatcgatc tggataactt caaggtgctc aatgacaccc ggggccacga tgtcggggat
     2041 aggctgctgg tcgaggcagc ccggcgtctg cgatcctgtg tgcgcgaagg cgataccgtg
     2101 gcccggctgg gcggtgacga gtttgtggtg atactggaag acctgagtgt gacggaggag
     2161 caggcggtag tgcaggcgga gatcgcgggg gagaagatac gcgcgaccct caatcagccc
     2221 tatcggctgg gcgcgatcga ccaccattgc tcttgcagca tcggcatcag cctgtttcat
     2281 gatcatcagg ttagcgtgga cgagttgctc aagcgcgccg acacggcgat gtacgaagca
     2341 aagaccgcag ggcgcaatgc gctgcgcttc ttcgatccgg cgatgcaatc tgcgctcgaa
     2401 gcaagagcgg cgctggaaaa tgagttgcgt caggcgctgc cgctgcggca gttcaggctt
     2461 tattatcaga aacaggtgaa tgtggacggt gatcctgtcg gtgccgaggc gttgctgcgc
     2521 tggatccatc ctgaacgcgg gctcgttcca ccgatggagt tcatcccgct ggcggaggaa
     2581 agcgaattga ttttgtcgat cgggctatgg gtgctggaaa ccgcctgtgc ccagatcaag
     2641 gcgtgggaat cgcacccctt gagccgcgag ctgcaactgg cggtcaatgt cagtgcgcgc
     2701 cagtttcatc aggccgattt tgtcgatcag gtgagggcgg tggttgaccg gttccgcatc
     2761 aacccggata gactcaagct ggaattgacc gagagtgtgg tgctggacga tatcaacgac
     2821 accatcgcga aaatgcgcga gctcaagcgg atcggggtgc gcttttccat ggacgatttc
     2881 ggcaccgggt attcgtcgct cgcctatttg acccagctgc cgctggatca gctgaagatc
     2941 gaccgttcct tcgtgagcaa catcggcatc aagtcaacgg atgcggtcat cgtgcaatcc
     3001 attatcaaca tggggcagaa tctgggcatg gatgtgatcg ccgaaggggt ggagaccgaa
     3061 gcgcaacgcg acttcctgat gcggcagggc tgtgatgcct atcagggctt tttgttcggc
     3121 aggccgcagc cactggatga gtttgaccgg cagctcggtt cgagttgagc cggttcgtga
     3181 gttcgccggg atttgtgccg gattgggcgg caaaacagcg ttgcgccggg cgatgcgggc
     3241 tgggcccgca ccctctcccc cgaggcagca gcgcccgccg tctgcgacag ggcgggcaaa
     3301 gcctgttaga atcgcgaact cagaattttc tgattaactt taatcatttg gagaaagtag
     3361 catggctgtt gaacgtaccc tgtcgattat caaacccgat gcagtagcga agaatgttat
     3421 cggtcaaatc actgcccgtt tcgagaacgc tggcctgaaa atcatcgctg cgcgcatggt
     3481 gcaattgtcc cgtgccgagg ccgaaggctt ctacgctgtg catgccgcac gtcctttctt
     3541 taaggatctg gtggacttca tgatctccgg cccggtgatg gttcaagtgc tggaaggcga
     3601 aggcgcaatt ctgaagaacc gcgacctgat gggcgctacc gatccgaaga aggcagagaa
     3661 gggcaccatc cgtgccgatt tcgccgacag catcgatgcg aatgccgtac acggttccga
     3721 cgctgcagaa actgctgccg tcgagatcgc ttatttcttc ccggcactga acgtctactc
     3781 gcgttaattc acgcttagtc agatgaagca aaacctcctc gacttcgatg tccagcaact
     3841 gactgcctgg tttgcggcca acggtgagaa accgttccgc gccaagcagg tgctgcgctg
     3901 gatttacaaa gccggggagt ccgatttcga tgcgatgagc gacctcgcgg tctctcttcg
     3961 cgaaaaactc aagcagatcg cctgtgtcca ggcgccgaaa gtgatgcgcg aagagctgtc
     4021 cgatgacggc acgcgcaaat ggctgctgga catgggcacc ggcaacgcgg tggagaccgt
     4081 gtatatcccc gaggagacgc gcggcacgct gtgcgtctcc tcgcaggccg gttgcgcgct
     4141 ggactgcgcg ttctgttcga ccggcaagca gggcttcaac cgcaacctca gcgtggccga
     4201 gatcgtcggc cagctgtggt gggcgaatca ccagctcggc aaggatgagg acggcaactg
     4261 gcgcatcagc aatgtggtga tgatgggcat gggcgagccg ctgctgaact tcgacaacac
     4321 cgtgagcgcg ttgcggctga tgctggacga tcacggctat ggcctgtcgc gccgccgtgt
     4381 gacggtgtct acctccggtg tcgtgccggc gatggaccgg ttgcgcgaag agtgtccggt
     4441 ggcgctggcc gtgtcgctgc atgcgcccaa cgacgcgctg cgcgatgtgc tggtgccgat
     4501 caaccggaag tatccgctca acgagttgat ggcggcctgt cggcgctatc tggaaaaggc
     4561 gccgcgcgac ttcattacct tcgagtacgt gctgctggac ggcgtgaacg acagcgagca
     4621 gcaggcgcgc gagctggtga aactggtgcg cgatgtgccc tgcaagttca acctgattcc
     4681 gttcaatcct ttcccgcaga cgcagtatca acgctccaag cccgaggcga tccgtcgctt
     4741 ccgcgatgtg ctgatgcagg cggatatcgt caccaccacg cgcaagacgc gcggcgagga
     4801 catcgcggcg gcctgcggtc agctggccgg acaggtgcag gataagacca aacgaaccat
     4861 gcaacgtata accgaggtgc attgatgaaa agacttttca ttctgtcgtt gttattgttg
     4921 tcggcctgcg gcaccccatc gggcggcggt tcccagccgc aaagcaatag tcgcggcacg
     4981 gccagcgcca agatccacac cgagctggcg gggatgtatt acgagcgttc gcagcttggc
     5041 atcgcgctgg ccgagatcga ggctgcattg caggcggatc gcaattacgc cccggcctat
     5101 caagtgcgcg gattgatcca tatgacgctg cgcgaggaca aggaggcgga ggaagacttc
     5161 aagcagagcc tgcatctgga caaggatgat tcggatacgc acaacaacta cggctggttc
     5221 ctgtgccagc gcgaccgggc gaaggagtct attcaccact tcatggctgc gctgaagaat
     5281 ccgctgtaca ccacgccgga gcgcgcctac ctgaatgccg gagtgtgttc tcagaaggcg
     5341 ggcaatctga aggatgccag tgagtttttg cagcgcgcct tgctgcttca gccgggtagc
     5401 ccgcaggccc tgatcgcatt ggccgagttg aattttgcca agggcgatta ttcggtggcg
     5461 aagaaatatt tcgccgaatt tgcccagcgg atcgagagcc cgagcgccga gcagctctgg
     5521 ttgggggttc gcatcgaacg caaggtgggg gatcgcaatg ctgcggccaa ttatgccgta
     5581 cagttgcgca agcgcttccc cgatgcgcag gaaactcaat ggctcatgca gggtgaataa
     5641 tggaacagca gaacgatact agtgtgaata cgaatgaagg cgcggctatg ccggtgctgc
     5701 ccggggcggg gttgcgcgag gcgcgcgaac gtctcggttt gagtgtggcc gatgcggccg
     5761 atcagaccaa gctcgcaccg cgccagatcg aggcgctgga agccgacgat tttgcacatc
     5821 tgccggaaat ggcgttcgtg cgcggttttg tgcgcagcta tgccaagatc ctgcaactgg
     5881 acccgcaacc cttgctggcc atgttgcctc aggcgatcgc gtcgggtgcg gctcaggtga
     5941 cgcggtcttc ggtcgaggtg cccttcccga atgcacattc gccacgggtg cagaatctgg
     6001 tctggctggg cgccgcgctg ttcttgtcgg tgctggtggt cgtgttcgcc gtttggcatt
     6061 acacctcgcc gcgcagccag cccgagccag agaagattcg cccggtcgag atggcgatcc
     6121 cgttgcctgt tgaggcgcat gtcgttgcgg cttcagaggt ggcagaagct tcggctgtcg
     6181 cggagcccga ggcaattgag ccgactccgc ccgttaatcc ggtgacgcct gccccggttc
     6241 agccggtcac gctggccaag cccgtcgccg cccagcctgc cgcgatcgct gtggttaaac
     6301 cggtgttgcc cgtcgctgcc tctgcggtta aggcgactca atctgcggca gcggcgacca
     6361 agccggccgt tgcggcgcct gcagcaaaac cgctttctcc cgttgcgccg cccgcagcga
     6421 agccgcttcc ggcggttgtc ccttccgcct cgtctgcacc gaccgtgttg cgactgactt
     6481 tcggcgagga gtcctgggcc gaggtgcgcg atgcgaaggg caatatcgtt tcttcccaga
     6541 tcaatccggc aggcagcgaa ttgaatctga aggggcaagg gccattctcg ctggtggtgg
     6601 ggcatgccag cacgtcgcgc ctgtttcata agggcaagca gatcgatctg acctctcatg
     6661 ccagcgccaa cagcgacgtt gcgcgtctga cgctggagta agcatgactg ccgtcctgat
     6721 caagcgccgc ccgtcgcaac gggtgcaggt gggtcgtgtg gcattggggg gcgatgcgcc
     6781 catcgtggtg cagtccatga ccaataccga caccgccgat atcggtgcga cggtgaagca
     6841 ggtctacgag ttggcgcaag ccggttccga gttggtgcgc atcaccgtga atacgccgga
     6901 agccgccgct gccgtccccg cgatccgcaa gcgtctggac gcattaggtt gctacgtgcc
     6961 gctgatcggc gacttccact acaacggaca ccgtctgctt accgactacc cggagtgcgc
     7021 gcagttgctg gcgaaatacc gcatcaatcc cggcaatgtc gggcgcgagc gcgaggaggc
     7081 agacccgttc acgatcatga tcgagacggc gatacgctac gaaaaaccgg tgcgcatcgg
     7141 ggtgaactgg ggcagtctgg accagcgtct ggtggtgcgc atgatggacg agaagtcgcg
     7201 tctgaccgag ccgaaagacg tgaaggaagt cacgcgcgcc gcgctgatcg cctccgccct
     7261 ggaaagcgcg caacgcgccg aacagctggg catgccgcat gaccgcatcg tgctgtcctg
     7321 caaggtcagc gaagtgcagg atctgatcgc ggtgtatcgc gcgctggcgc ggcaatgcga
     7381 ttacgcgctg cacctcggac tgaccgaggc gggcatgggc tccaagggaa tcgtcgcttc
     7441 cactgcggcg ctctcggtgc tgttgcagga aggcataggc gacaccatcc gcgtgtcgct
     7501 gacacccgcg ccgggcggtt cgcgcaccga ggaggtgatc gtcgcgcagg agatattgca
     7561 gaccatgggg ctgcgcgcgt tcgcacccat ggtcaccgcc tgccccggtt gcggacgcac
     7621 caccagcacc gtgtttcagg agctggcgca gagcatccag gcgcatctgc gcacgcaaat
     7681 gccgctgtgg cgccgtcagt atgacggcgt ggagaacatg accgtggcgg tgatgggctg
     7741 cgtggtgaac gggccgggcg aaagcaagct ggcgaacatc ggcatcagcc tgcccggcac
     7801 cggcgagacg cccgccgcac cggtctatat cgacggcgaa aaggcgatga ccctgcgcgg
     7861 cgacaatatc gcggcagagt tcacccggat cgtggacgag tatgtggaac gaaaatacgt
     7921 taagaaagaa atggagagta gtgagtagga agcggcaagt gggagggtat tccctgctcg
     7981 ccacttactg ctttccgctt gctggatttg gaatatgagc aaagaaacac tgcaagccgt
     8041 aaaaggcatg aacgacatcc tgccggaaga ggcggagctt tggctctggt tcgaggaggt
     8101 ggtgcgcgaa tggctgcaca gctacggcta ccgcaacatc cgcatgccgc tggtcgagcc
     8161 gaccgcgctg ttcaagcgcg ccatcggcga ggtcaccgac atcgtcgaga aggagatgta
     8221 cagcttcgag gatgcgctca acggcgacca tctgacgctg cgcccggaag gaaccgcctc
     8281 ctgcgtgcgc gcagtgctgc agcacaattt gctgtataac gcgccccagc gtctgtatta
     8341 cagcggccag atgttccgcc acgagcgtcc gcagaagggg cgttaccgcc agttccatca
     8401 ggtcggcgtg gaggcgctgg gcttcgcagg ccccgatatg gatgccgagc agatcatcat
     8461 gtgcgcaagg ttgtggcgca ggctgggtat ccgcgatgtc acgctgcagc tgaatacgct
     8521 gggggatagc gcctcgcgtc atcgccatcg cgagaagttg atcacctatt tcgagcagca
     8581 cgagtcgctg ctggacgcgg acgcgacgcg ccgtttgcac agcaatccgc tgcgcatcct
     8641 ggacagcaag aatccggcga tgcaggagct gatcgcgggt gcgccccagt tgatggacga
     8701 gctggaacag gatgcgatcg atcatttcga ggcggtgcag gcgatcctga aacgtcacga
     8761 catcgcgttc gagatcaacc cgcgcctggt gcgcgggctg gactattaca atcgcacggt
     8821 gttcgagtgg gtcaccaccc gtttgggcgc gcagggcacc atctgtgcgg gcggccgcta
     8881 cgacggcctg atcgaacaga tcggcggcaa gcccgcaccg gcgatgggtt ttgcgatggg
     8941 cgtggagcgc ctgctggcgc tggtgctgga agacggcatg acgccgccca aaccggaggt
     9001 cgatgtgtat gtggtgcatc agggcgatca ggcagcgcga ctggccagcc aggtggcgga
     9061 gcagctgcgc gacgacaagt tgcgcgtgct gctgcattgc ggcggcggca gtttcaagtc
     9121 gcagatgaaa aaggcggacg ccagcggtgc gcgcatcgcg gtggtcatcg gcgacgatga
     9181 ggcgctggcg aaccaggtca gcgtcaagcc gatgcagggc ggcgaacagt taagggtggg
     9241 cgtgggccag ctcatcgaaa cgatcaacaa cattatcaag acgaggtaga acatggcggc
     9301 attggacttg caggaacagg aacagatcga cgcgctcaag gcgtggtgga aggacaacgg
     9361 caagtacgtg ctcggcgcag tgctgctgct ggtcatcgca ttcggcggct ggcgcggctg
     9421 gcagtcccac cagaaaaaca agaacgtgga ggccgcgacg ctgtatgcgg aattcacccg
     9481 gcaactggag agcaatgacg ccaagcgcgt caacgacgcg gctgcggcgg tgatgggcaa
     9541 gtacgcggat agcgcctatg ccgcgcatgc ggccttgctg gcagcgcagt tcaacgagtt
     9601 caagaaggat gtggcgcacg ccaagacgca attgcagtgg gtgatcgacc atgccgacga
     9661 agcatcgctg aaggatgtgg cgcgtctgcg tctggccgcg atcctgctgg acgagaagag
     9721 ttacgatgcc gcgatgaaat tgctgaatgc cgcgcatccg gattcgttca atggcctgta
     9781 ttccgacctg aagggcgatg tgctgaatgc gcagggcaag accgacgagg cgagaacggc
     9841 ttacaaactg gcctttgaca agaccgatgc caagagcatg taccgcaatc tgatccagat
     9901 gaagatggat gcgttgggag tggcaaagtg atttcgcggc ctgttcgact cggctcgggg
     9961 gcattgctgt tgacggcgct gttcgccttg agcggctgtg aaactttgca gggctggatc
    10021 ggcaagaaag acccactggt cgaaccggcc aagctggaag atttcgccgc gagtgccaaa
    10081 ttcgaggtgc gttggcacac ggatatcggc agcgccaagg agaatctgct gcaaccggca
    10141 gtgacccgtg atgcgatcta cggtgcttct ggcgagggcc ggttgacgcg tctggatcgt
    10201 gccagcggca aacaggtgtg gcgcatcgaa agcggcatca gggtgaccgg cggcgtcggt
    10261 gcgggcgaag gtctggtgct gatcggcagc gataagggcg aggtgctggc tttcgatgaa
    10321 aatggcaagc cgcgctggaa gagtcaagtc tccagcgagg tgttgagcgt gccgcaagta
    10381 tcggacggtg tggtgatcgt gcgcagcggc gacgggcgca tcgccggact gaacgcggcg
    10441 gatggcaaac ggatctggct gtacgagcgc agcacgccga cgctggtggt gcgtagccat
    10501 gcgggtgtga ccatacagaa cggggtggcg ttcgcgggct ttgccggcgg gaaactgctg
    10561 gcgttaaagg tcaagaatgg cgatgccctg tgggagtcgg ccgtatcgtt gccgcgcggc
    10621 aacaccgagc tggagcgtat cagcgatatc accagcgatc cggtggccga tgatcagcag
    10681 gtttgcgcca tcgcctttca ggggcgggtg gcctgctatg acgtcgcgca gggtaactcc
    10741 ctgtggagtc gcgatatctc cagcgacaag gggctgatgt tgatgcgcaa gtatctctat
    10801 gtgaccgatg ccgccggaac cgtcatggcg ctggacaaga tcagcggcag cacgctgtgg
    10861 aagaacgagc aattattcat gcgcgatacc gctgcgccct atgcgctggg cgactccgtt
    10921 gtggtgggtg attacgaggg ttatctgcat ggcctgagcc gtgaggatgg ccatttcacc
    10981 gcacgcatca aattgggaag taagattcag gctgctccgc agccgctgga tgacggtctg
    11041 ctggttcaga cacgcgatgg cgacttgtat tcgctgtcgt tacattaagg aactatttta
    11101 aatgctgcct acagtagtct tggttggtcg tccgaatgtg ggcaagtcca ctctgtttaa
    11161 tcgtctcacg cgcagccgcg atgcactggt cgccgaccag cccggcctga cgcgcgatcg
    11221 tcactatggc cgtggccggg tggggacgcg tccctatctg gtggtcgata ccggcggact
    11281 ggagccggtc gccaaggacg gcatcctgta cgagatggcg cgtcagagcc gtcaggcggt
    11341 agacgaggcc gacgtggtct tgttcatcgt cgacggacgt gccggttgta ccccgcagga
    11401 catgatcatc gccgagcagt tgcgcaagac cggcaagcct atcctgttgc tggtcaacaa
    11461 ggccgagggc atgcagcgcg cccgtgtgac gatcgatttc ttcgagctgg ggctgggcga
    11521 accctttccg atctcctccg cgcatggcga caacgtggaa gaggtggtgg agctcgcgct
    11581 ggagaacttc cctgccgagg tcgagcccga ggaaaaagaa gagcagcacc ccaagatcgc
    11641 catcgtcggt cgtcccaacg tcgggaaatc cacgctggtg aatgccatcc tcggcgagca
    11701 gcgcgtgatc gcgttcgacc agccgggcac cacgcgcgat tccatctata tcgacttcga
    11761 gcgcgacggc aagcaataca ccatcatcga caccgcaggc gtacgccgtc gcggcaaggt
    11821 cgacgaggcg atcgagaagt tctcggtgat caagaccatg caggcggtcg aggatgccaa
    11881 cgtggtggtg ctggtggtgg atggtgctga ccagatcacc gagcaggacg cgcacgtcgc
    11941 cgacttcgtg ttgcaggccg gacgtgcgct ggtgctggcg gtcaacaaat gggacgggct
    12001 ggatgcgcat cagcgcgaga cggtcaagag cgacatcgag cgcaagctgc atttcctcag
    12061 tttcgccaag ctgcatttca tctccgcact gaacggtagc ggggtggaca aggtgctgaa
    12121 gtcggtggaa gaggcctacg cggcggcgat gatcaagctg ccgacgccga agctgacgcg
    12181 cgccctgctg accgcactgg agaagcagca gccgcccaag gg
//
LOCUS       DASUIV010000254         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1366124, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000254 DASUIV010000000
VERSION     DASUIV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366124"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFW59_09170"
     CDS             <1..218
                     /locus_tag="VFW59_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364429.1"
                     /translation="NSEHDCESRAQNIKVACDLLVLWGKRGVVSKLFKPLELWQAQCA
                     GRDHGTRAAGRTFHPGGIATTHCGGTA"
     gene            complement(435..2249)
                     /locus_tag="VFW59_09175"
     CDS             complement(435..2249)
                     /locus_tag="VFW59_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HEX5364430.1"
                     /translation="MYQQIYDPLGNAFFSTLVAAIPILVLLYFIALHRYRDSQGNVHL
                     GISAPYAAFFGVIAAFLVSCLVFKMPIISAVSAFALGSLSGFLGIIWIVLAAMFLYTM
                     SVVAGKFEIVKESIVHISFDRRLQCVLIAFSFGAIIEGTSGFGTPVAIAGAVMVGLGF
                     KPFQSAVLNLLANTAPVAWGAIGTPIVTLAAVSGLDQVTLSAMAGHQLPFVSILVPFW
                     LVATFVKMEGGTWKEAFEVWPAALCAGASFALMQFLASGTNEFHLMTDVVSGVFSVVC
                     TALFLRFVWHPKTRFLLKSEREALTKAGKSTATATTDSTEWKYSYTTKETVHAWLPWV
                     ILILCCALWGMPEFKKYLNNLFSGVTFATTLLGSPFAGTLSLPVWDMPALHNLVQRMP
                     PVAAINAKPEAARFVINWLSAAGTGVFVAAILSGLVLKLNAAQWKEALVQTLHRMKIP
                     VLVIGQVLGLGFLTRYAGTDAVLGLAFTGAGFMYPFFAAYLGWLGVFLTGSDTASNAL
                     FGSLQRITAEQLNLNPILIVATNSTGGVMGKMIDAQSIMVACAACYDDPKERAYALGP
                     IFRTVFWHSIAGAAVIGVIAMLQAYVFPGMIPIPPLGK"
     gene            complement(2695..3009)
                     /locus_tag="VFW59_09180"
     CDS             complement(2695..3009)
                     /locus_tag="VFW59_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364431.1"
                     /translation="MSARLEVRLSSEVQDGRLKSGRFYTRANLARIATDAMLERRLEP
                     EFSAEVQQQLAQIASTGAEAGADIRDLTARLWCSIDNDNSCDLDQFTVCEVLPRGSVK
                     IR"
     gene            complement(3019..>3741)
                     /locus_tag="VFW59_09185"
     CDS             complement(3019..>3741)
                     /locus_tag="VFW59_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEX5364432.1"
                     /translation="TRYDETPQWEFCEGFMAAVICSRRPIAAAEYLPVLLGTPAEGEE
                     PDDESGSFASEAQRERFLALWNQRWNEVVTALDAEVKSLEDEHCYHPEVMDIRGAVAE
                     MPAEEQAAFKGEELPAFAQVWALGFMFAVESWPEEWAAPRDKDAARWLDGALQAVVAM
                     TEDDEDAPELSPLSEDGAPSTSIARLNAFGEAIWAVYDLRELWKTLGPRVETIRKDAA
                     PGRNDLCPCGSGKKYKKCHGAT"
BASE COUNT          814 a         1137 c         1095 g          695 t
ORIGIN      
        1 taaactcgga acacgactgc gaaagccgtg ctcagaacat caaggtggcc tgcgacctgc
       61 tggtgctctg gggcaagcgc ggcgtggtca gcaagctgtt caagccgctt gagctctggc
      121 aggcgcagtg cgcgggccgg gatcacggga cacgcgctgc cggccggaca tttcatcccg
      181 gaggaattgc cacaacccac tgcggcggca ctgcttgatt tttttagccc agacaaatag
      241 gcattcaggt caatttctcg accttgacgc cattttttga gttttcacac aggcttagcc
      301 tgttctggac ggtagtgctt ctggaaagcg gtcactcatt cgctgacggg aggggccttt
      361 cgcccactaa agagaagagc gccgcccttt gcctcaggtc aaggcgacat aacccggatt
      421 tggcgcttca cgcctcactt gccgagcggt ggtatcggaa tcatccccgg gaaaacatac
      481 gcctgcagca tagcgatcac gccgatcacc gcagctccgg cgatcgagtg ccaaaacacc
      541 gtgcggaaaa ttggccccag cgcatatgcc cgctctttcg gatcgtcata gcatgctgca
      601 caggccacca tgatggactg ggcgtcgatc atctttccca tcacgccgcc ggtcgagttc
      661 gtggccacaa tcagtatcgg attcaagttg agctgttcgg ccgtgattcg ttgcaggctg
      721 ccgaacagcg cgttggaggc ggtgtcggaa cccgtgagga aaacaccaag ccagcccaga
      781 taggcggcga agaaggggta catgaagccg gctccggtga acgcgaggcc gaggaccgca
      841 tcggtaccgg cgtaacgcgt gaggaagccg agcccgagca cctgaccaat caccaggacc
      901 gggatcttca tgcggtgaag ggtttgaacc agagcctcct tccattgtgc cgcgttcagc
      961 ttgagaacta gccccgacag aatcgccgcg acgaacacgc cggttccggc agctgacagc
     1021 cagttgatga caaacctcgc ggcctccggt ttggcgttga tcgcagcgac cggcggcatc
     1081 cgttgcacca gattgtgcaa cgccggcata tcccagaccg gcagcgagag cgtgcccgcg
     1141 aaaggtgaac ctaacagcgt cgtggcgaag gtgacgccgg aaaagaggtt attcagatac
     1201 ttcttgaatt ccggcatgcc ccacagcgcg cagcaaagta tcaatatcac ccacggcaac
     1261 catgcatgca ccgtttcctt ggtcgtatag gaatacttcc attcggtgct gtcggtggtc
     1321 gctgtcgctg tgctcttgcc ggctttggtc agcgcttcgc gctcggactt gagaagaaag
     1381 cgagtcttcg gatgccagac aaagcgcagg aacagtgcgg tgcaaacgac cgagaacaca
     1441 cccgagacca catcggtcat caagtgaaat tcgttggttc cagaagcgag gaactgcatc
     1501 agcgcaaacg aggcacccgc gcacagcgcc gccggccaca cctcgaacgc ttccttccag
     1561 gttccgccct ccatcttcac gaacgtggcg accagccaga acggaaccag aatcgagacg
     1621 aagggcagct ggtgacccgc catggcggaa agcgtgacct ggtccagacc gctgaccgcg
     1681 gccagcgtca cgattggcgt gccgatggcc ccccacgcta ctggcgcggt gttggccaga
     1741 agattcagga cggcggactg gaacggtttg aaccccaggc ccaccatgac tgcaccggcg
     1801 atggccaccg gcgtcccgaa gcccgaggtt ccctcgataa tcgccccgaa cgaaaacgcg
     1861 atcagcacgc actgaagtcg ccggtcgaat gagatgtgaa cgatggactc cttcacgatc
     1921 tcgaacttgc ccgctaccac actcatcgtg tacaggaaca tcgcggccag aacgatccag
     1981 atgatgccca ggaaccccga gagcgaccca agcgcaaagg ccgacacggc cgagatgatc
     2041 ggcatcttga agaccagaca ggagaccagg aacgcggcga tgacgccaaa gaacgccgca
     2101 tagggcgcag agatgccgag gtgcacattg ccctgggagt cgcggtaccg gtgcagggca
     2161 atgaaataca ataaaacgag aatcgggatc gcagctacca aggttgagaa aaatgcattc
     2221 cctaatggat catagatttg ctgatacata gcttctcctt tttgattgaa ttacgtgccc
     2281 gacattcctc ttgaatcaac cgttccgaac gaaaataatg cgcaccatac ccgcaaaacg
     2341 acaaatagac aaatagacaa ggttggcggc gttcgggctt caaggatcag atcgcgagat
     2401 tgcctatgaa gctggtaatc cagctggcaa gtgagctggt agcccgtctt tgaaggagag
     2461 cggttcgggg gactcacgat cccgcaagaa ctactgtcac aagccgaccg ggggatcgaa
     2521 tagtccgtcc tgaccagaaa atctggcgag cgtctgctac gaagcaagcg cgtgactgac
     2581 cgttggccgg catcggaaac aagcgtgccg ctgtgtaacc caatgtcgtg ttggttcgcg
     2641 catgctcatc catggctgtg cctatcttga cgaggacatc tacatcggca atggctaccg
     2701 gatcttgacc gagccacggg gcaacacctc gcagaccgtg aactgatcga gatcgcacga
     2761 gttgtcgttg tcaatcgagc accaaaggcg cgccgtgaga tcgcgaatgt ctgcgccagc
     2821 ctcggcgccg gtgctggcga tctgcgcaag ttgctgctgg acttccgctg aaaattcagg
     2881 ctccagcctc ctctcaagca tggcatctgt tgcgatgcgg gcgaggttag cccgggtata
     2941 gaagcgccct gatttcaggc ggccatcttg cacttcactt gaaaggcgca cttcaagcct
     3001 tgcagacatg gcatgcgctc acgtcgcccc gtggcacttc ttgtatttct tgccgctgcc
     3061 gcaggggcac aggtcattgc ggccgggcgc cgcatccttg cgaatggttt ctacacgcgg
     3121 ccccagcgtt ttccacagtt cgcgcaggtc gtacaccgcc cagatcgctt cgccaaaggc
     3181 attgagccgg gcaatgctgg tgctgggggc accgtcttcg ctgaggggtg acagctcagg
     3241 cgcgtcttcg tcatcttcgg tcatggcgac cacggcctgc agcgcgccgt ccagccatct
     3301 ggcggcgtcc ttgtcgcgcg gtgctgccca ttcctcgggc cagctttcca cggcaaacat
     3361 gaagcccagc gcccacacct gcgcaaacgc gggcagctct tcgcccttga aagcggcttg
     3421 ctcttcggcg ggcatctcgg caacggcgcc gcgaatgtcc atgacttcgg ggtgatagca
     3481 gtgctcgtct tccagcgact tcacttcggc gtcgagcgcc gtgacgacct cgttccagcg
     3541 ctggttccag agcgcgagga agcgctcgcg ctgcgcttcg ctggcgaacg agccgctttc
     3601 atcgtcaggc tcctcgccct cggcgggcgt gcccagcagc accggcagat attcagcggc
     3661 ggcgatgggg cggcgcgaac aaatcaccgc agccatgaag ccttcacaaa actcccactg
     3721 cggcgtttca tcgtagcggg t
//
LOCUS       DASUIV010000255         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_65118, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000255 DASUIV010000000
VERSION     DASUIV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65118"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /gene="sucA"
                     /locus_tag="VFW59_09190"
     CDS             <1..897
                     /gene="sucA"
                     /locus_tag="VFW59_09190"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293302.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5364433.1"
                     /translation="DFVVIDSLLSEEAVLGFEYGYATAEPQSLVLWEAQFGDFANGAQ
                     VVIDQFIASGEVKWGRMCGLVMLLPHGYEGQGAEHSSGRIERYLQLCADYNIQVCIPS
                     SPAQMFHLLRRQMLRPMRKPLIVFTPKSLLRSKDAASPLAELTQGRFLPVIPEAEALD
                     GGKVRRIIACSGKVYYDLLNARRAKGISDMAIIRIEQLYPFPHDDFAAQIAAYPNAAE
                     LVWCQEEPGNQGAWHRIQHYLLRHLRPGMRLDYALRPSSAAPAAGYLAVHQEQQKAVI
                     DAAFRDDLDNKPNPGAAHHEHH"
     gene            884..2053
                     /gene="odhB"
                     /locus_tag="VFW59_09195"
     CDS             884..2053
                     /gene="odhB"
                     /locus_tag="VFW59_09195"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX5364434.1"
                     /translation="MSIIEVKVPQLSESVTEATLLTWHKQVGESVAEGENLIDVETDK
                     VVLELPASKSGVLCKIIKGDGEKVGSEEVIAQIDTAAVVQAAAAPADVSPAVPPSVRK
                     LAREHEIDAAALQGSGRSGRVTKEDVLNAMQQVSSAQVSSIQAPQAVPAIAPSGNRPE
                     QRVPMTRIRQRIAERLLQSQQNAAILTTFNEVNMQPVIELRNRYKDAFEKKHGVKLGF
                     SSFFVKAAVLALQKFPVVNASVDGSDIIYHGYYDIGVAIGSERGLVVPILRDADKLSF
                     ADIEKQIADFGTRAKVGKLTMEELSGGTFSISNGGVFGSMLSTPIINPPQSAILGIHA
                     TKDRAVVENGQIVIRPMNYLAVSYDHRIIDGREAVQFLSTIKEALEFPGRVLLDL"
     gene            2063..3055
                     /locus_tag="VFW59_09200"
     CDS             2063..3055
                     /locus_tag="VFW59_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX5364435.1"
                     /translation="MSSFSAYRIFEEDGKSSGRFVELQLADLDAGEVVIRTHYSGVNY
                     KDALAATGAGKVIRRFPCVGGVDAAGVVESSSDTRFKAGDEVLVTGYGMGVSHDGGYA
                     EYVRVPADWVVPLPSGLSMYDAMALGTAGFTAALSIHRLEQNELRPENGKVIVTGATG
                     GVASLAIQMLAQLGYHVVALTGKDAEHDYLKSLGAAEILSRKDVQMGVRPLEKAQWAG
                     ALDAVGGETLAWLTRTMQQDGAIASFGNAGGIELHTTVMPFILRGVRLVGIDSAATAM
                     PLRRKIWQRLASDLRPRLLAQAVHTVPFSALPEVFPKMLQGGSCGRAVISFPAV"
     gene            3276..3360
                     /locus_tag="VFW59_09205"
     tRNA            3276..3360
                     /locus_tag="VFW59_09205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3310..3312,aa:Leu,seq:tag)
     gene            3496..3858
                     /locus_tag="VFW59_09210"
     CDS             3496..3858
                     /locus_tag="VFW59_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364436.1"
                     /translation="MQPIEHYLGPQYSAAVRVRNVASYLATALLLVAIWIFFAGLVCG
                     TLVHFGLGVPAAIVLVQAMMVIGGIVIVAMLPRIKIRNRRYVGLPIFKPTAAGKDPFN
                     SFVTAPPVTPPRFISYAI"
     gene            3940..4251
                     /locus_tag="VFW59_09215"
     CDS             3940..4251
                     /locus_tag="VFW59_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364437.1"
                     /translation="MQQLNSTIQILLARFNGQVLIPFVAASEAAGIPEQTARNRLAKG
                     DYPIPTVLNGSRRFIHVADLSEFIDSLRSPKPQRGRPRKTASASASASASASASASAS
                     R"
     gene            4255..>4851
                     /locus_tag="VFW59_09220"
     CDS             4255..>4851
                     /locus_tag="VFW59_09220"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5364438.1"
                     /translation="MSVKIMGMVFEFYPVGGGEMLLALSLADFADDDGGRIFPSVPTL
                     ARKTRQSERSVQYQLRKMEQSGFIQVVERSRGGVVRGKKYRSNEYRINIGVLSNGAKT
                     APLNQRCNLAQSTVQSDASNGAIAVAPNPPLEPSLEPPLPPLIFPSAVTPEEKTVIGG
                     LMGALPENAKQELLDELAGAIRANAIRRGFVPFIRGLVD"
BASE COUNT         1010 a         1351 c         1439 g         1051 t
ORIGIN      
        1 gatttcgtcg tgatcgattc gctgctgtcc gaagaggcgg tgctgggttt cgaatacggc
       61 tacgccaccg ctgagcctca atcgttggtg ttgtgggaag cgcagttcgg cgactttgcc
      121 aacggcgcgc aagtggtgat cgaccagttc atcgcgtccg gcgaagtgaa gtggggccgg
      181 atgtgcggtc tggtcatgtt gctgccgcac ggctacgaag ggcagggcgc ggagcattcc
      241 tcggggcgca tcgaacgcta tctgcaactg tgcgccgact acaatattca ggtgtgcatc
      301 ccgtcgagcc cggcgcagat gttccacctg ttgcgtcgcc agatgctgcg cccgatgcgc
      361 aagccgctga tcgtgtttac ccccaagagt ctgctgcgca gcaaggatgc ggcttcgccg
      421 ctcgccgagc tgacgcaagg ccgcttcctg ccggtgatcc cggaagcgga ggcgctggac
      481 ggcggcaagg tgcgccgcat catcgcatgc agcggcaagg tgtattacga cctgctcaac
      541 gcgcggcgcg ccaagggcat cagcgacatg gcgatcatcc gcatcgaaca gctatacccg
      601 ttcccgcacg acgacttcgc cgcgcagatt gccgcgtacc caaatgccgc agaactggtc
      661 tggtgtcagg aagagccggg caatcagggc gcatggcatc gcatccagca ttatctgctg
      721 cgccatctgc gccccggcat gcgcctcgac tacgcgctgc gcccgtcgtc tgccgcacct
      781 gctgcgggct atcttgcagt acaccaggaa caacagaagg cggtgatcga tgccgccttc
      841 cgcgacgatc tcgataacaa acctaatccg ggagctgcac accatgagca tcattgaagt
      901 caaagtaccg caactgtccg aatccgtcac cgaagcgacg ttgctgacct ggcataagca
      961 ggtcggcgag tcggttgccg agggcgagaa tctgatcgat gtcgaaaccg acaaagtggt
     1021 gctggagctg cctgcttcca aaagcggcgt actgtgcaag atcatcaagg gcgacggcga
     1081 gaaggttggc agcgaagagg tgatcgcgca gatcgatacc gcagcggtgg tgcaagccgc
     1141 agcagcacct gccgatgtgt ccccggccgt gccgccatcg gtgcgcaaac tggcgcgcga
     1201 acatgagatt gatgccgccg cactgcaagg cagcggacgc agcgggcgcg tcaccaagga
     1261 agatgtgctg aatgcgatgc agcaggtttc ttcggcccaa gtgtcgtcga tccaggcacc
     1321 gcaggccgta cccgccatcg cgccgtccgg caaccgtcca gaacagcgcg tgccgatgac
     1381 gcgcatccgc cagcgcatcg cagaacggct gctgcaatcg cagcagaatg ccgcgatcct
     1441 gactacgttc aacgaagtca acatgcagcc ggtgatcgag ctgcgcaacc gttacaagga
     1501 tgcgttcgag aagaagcacg gcgttaagct cggcttttcg tcattcttcg tcaaggcggc
     1561 ggtgctggcg ttgcagaagt tccccgtcgt caacgcgtcg gtggacggct cagacatcat
     1621 ttatcacggc tattacgaca tcggcgtggc catcggcagc gaacgcggtt tggttgtccc
     1681 catcctccgc gatgccgaca agctgtcctt cgccgacatt gaaaaacaga tcgccgactt
     1741 cggcacgcgt gccaaggtcg gcaaactgac catggaagag ctcagcggcg gcaccttctc
     1801 gatttccaat ggcggtgtgt tcggttcgat gctctcgact cccatcatca atccgccgca
     1861 gtccgccatc ctcggcatcc atgccaccaa ggaccgggct gtggttgaaa acggccagat
     1921 cgtgatccgt ccgatgaact acctcgcggt atcctacgat caccgcatca tcgacggacg
     1981 cgaagcggtg cagttcctgt ccaccatcaa ggaagcgctg gaattcccgg ggcgcgtgct
     2041 gctcgacctg taaaggttac tgatgtcgtc attttccgct tacaggatat tcgaggagga
     2101 cggcaagtcc tccgggcgct tcgtcgagct tcagcttgcc gatctcgatg ccggtgaagt
     2161 cgtcatccga acgcattatt ccggcgtaaa ttacaaagac gcgcttgccg ccaccggtgc
     2221 aggcaaggtg atacggcgtt tcccttgcgt gggcggggtg gatgctgcgg gcgtggtgga
     2281 aagttcgtcc gacactcgat tcaaagcggg cgacgaagtg ctggtcaccg gctacggcat
     2341 gggcgtgagt cacgacggcg gttacgcgga atacgtgcgc gtgcccgccg actgggtggt
     2401 gccgctgccg tcggggctgt cgatgtacga cgcgatggcg ctggggacgg caggttttac
     2461 cgccgcgctg tccattcatc gtctggagca aaacgaactg cgccccgaaa acggcaaggt
     2521 aattgtcacc ggagcaaccg gcggcgtggc gagcctcgcg atccagatgc tggcgcagct
     2581 cggctatcac gtcgtggcgc tgaccggcaa agatgccgaa cacgattacc tcaaatcgct
     2641 cggtgcggca gaaattttgt cgcgcaagga tgtgcagatg ggcgttcgtc cgctggagaa
     2701 agcgcaatgg gccggtgcgc tggatgcggt agggggcgag acgctggcct ggctgacgcg
     2761 caccatgcag caggatggcg cgattgccag cttcggcaac gccggcggca tcgaattgca
     2821 taccacggtg atgcctttca ttttgcgtgg agtgcgcctg gtcggcatcg actccgcagc
     2881 gacagcgatg ccgttgcgcc gcaaaatctg gcagcggctg gcgagtgact tgcgtccccg
     2941 gctgttggcg caggcggtgc ataccgtgcc attttccgca ttgcccgagg tcttcccgaa
     3001 aatgttgcaa ggcggatcgt gcgggcgagc cgtaatcagc ttccctgctg tttagcagat
     3061 aggccgtttg ctggcataag ctcgcggtga aaacgtccgc cacgcaactc ttcgcatccg
     3121 gctttcagta gtttctcccg caatcaggaa ttcgtcgtta tgtggtgcaa gcagcctgcg
     3181 agttgcttgc ctgcgtttaa tgtagtggtt tatcagtgaa gcgaaagctt acagagtgat
     3241 gctataatct tgccccatag ctggaagcat acagcgcgaa agtggcggaa ttggtagacg
     3301 cactggattt aggttccagc gccgcaaggc gtaagagttc gagtctcttc tttcgcacca
     3361 agctaaatca atgagttaga tatactgcgg gataccccgg tatcctgtca actcccgaag
     3421 ttttccaagt aatttctcca gaattttcca tgaattttct tgcgcaaata gggtatgtgc
     3481 tggtattgtt ttggcatgca acccatcgaa cactatctgg gacctcaata tagcgctgcc
     3541 gttagggtgc gcaatgtggc gagttatctc gctacggcgt tgctgctggt cgcgatttgg
     3601 atattttttg ctgggctggt ttgcggaact ttagttcatt ttggtcttgg tgttccggct
     3661 gccattgttt tggtgcaagc gatgatggtg attgggggga tcgttattgt agcgatgctt
     3721 ccacggatta aaatccgtaa taggcgatat gttggcttgc caatcttcaa gcctactgcc
     3781 gcaggcaaag atccctttaa ctcattcgta acagcccctc cggttactcc ccctcgtttc
     3841 atctcctacg cgatttaaat gcgcccgttt agggctggtt tattcgtgat caggagaagc
     3901 tgactgcttg tctcctggtc gcgcgccaag gagacgcaaa tgcagcaact taattctacg
     3961 attcaaattt tgctggcccg gtttaacggt caagtcctaa tcccatttgt ggcggcctct
     4021 gaagccgcag gtatccccga gcaaaccgcc cgaaaccgct tggcgaaagg cgattatcca
     4081 attcctaccg tattgaacgg ctcgcgccgg tttattcatg tggcggatct ttctgagttc
     4141 attgattcgc tgcgctcgcc taagccccag cgaggtcgtc cacgcaagac agcatcagca
     4201 tcagcatcag catcagcatc agcatcagca tcagcatcag catcgaggta atccatgtcg
     4261 gtcaagatca tgggcatggt gttcgagttc tatccggtag gcggcggaga gatgttgctg
     4321 gcgttatctc tggccgattt tgctgacgat gatggcggac gaatatttcc tagcgttcct
     4381 acactcgcac gcaaaacacg ccaatcggag agaagcgtcc agtatcaact tcggaagatg
     4441 gagcagtctg ggttcatcca ggtggtggag agaagtaggg gtggtgtcgt tcggggaaaa
     4501 aagtataggt cgaacgaata ccgcataaac attggggttc tgagtaacgg tgcaaaaact
     4561 gcacccttaa atcaacggtg caatttagcg caatcaacgg tgcaatctga cgcatccaac
     4621 ggtgcaatag ctgttgcacc caacccacca ttagaaccat cacttgaacc accactaccg
     4681 ccgctcatat ttccgtcagc agtcacgcca gaggaaaaga cggtcatagg tggattgatg
     4741 ggggctcttc ctgaaaacgc gaagcaagag ctgctggatg aattggctgg cgcgatacga
     4801 gcaaatgcaa ttcgtcgtgg ttttgttcca tttattcgcg gtttggtcga t
//
LOCUS       DASUIV010000256         9340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_346465, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000256 DASUIV010000000
VERSION     DASUIV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346465"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..277)
                     /locus_tag="VFW59_09225"
     CDS             complement(23..277)
                     /locus_tag="VFW59_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364439.1"
                     /translation="MALLKNAPSGKITNANIVGNVQIGGETMIENQGEIEHSNIKSNI
                     QIESPPTKPKRWWLHLAESIVVSVIAGLILLAITIYLGLR"
     gene            complement(430..903)
                     /locus_tag="VFW59_09230"
     CDS             complement(430..903)
                     /locus_tag="VFW59_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX5364440.1"
                     /translation="MSQPSFLCDEMLGHLCRYLRAAGYDTLLANDSASDADLLRQCSE
                     EGRYFLTQDTLVREHKAACGIALILPHAPLDRLAELVDARFHLDWLEHAFTRCLIDNT
                     PLVQADEAATARIPDDARHPDQPLLHCPKCGRVYWRGSHYKRMHARLEAWQQKKG"
     gene            1189..1773
                     /locus_tag="VFW59_09235"
     CDS             1189..1773
                     /locus_tag="VFW59_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX5364441.1"
                     /translation="MEHTLPALPYAMDALQPHMSKETFEYHYAKHHQAYVTNLNNLIK
                     GTEFENLDLEAIVKKAPAGGVYNNAAQVWNHTFFWNCMKPNGGGAPTGALADAINKKW
                     GSVDEFKKVFQASAVGNFGSGWTWLVKKADGSVDIVNMGAAGTPLTTGDKALLCVDVW
                     EHAYYIDYRNMRPKFVETYLNNLVNWDFVAKNFA"
     gene            complement(1837..3099)
                     /locus_tag="VFW59_09240"
     CDS             complement(1837..3099)
                     /locus_tag="VFW59_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5364442.1"
                     /translation="MIKKVRVDELQPGMFIHDLNCGHVFHPLLHTQFHLDDTRAIDRL
                     IERGVSSVYIDTRLGRDAPDAPTAQEVRDDIDQTLLNMAQRNASADTHPAPLSASDID
                     LAHQVYEEASLTVHELMTTTRLGKHAELEQVRPAVQGIVDSIFRNADPLLHLGTIRNA
                     NQYLFYHSVATCSLLSAFAQQLGIGRDITRQLAIGAMLHDIGKTRIAESILDKPGRLT
                     DAEFDEIKRHVEYGMEILADAPWLTPISLQVVAQHHERYDGGGYPAALKSDHLSQYGQ
                     MAAIVDVYDAITSARSYHAAMEPVEAIRKLREWSEQHFNVELVDHFICCVGIYPTGTL
                     VRLESGLLGIVATHNPANQLKPAVLVTYDTNRSCRIKPYELDLATQSGDRIVGYESAE
                     KWGIDPNEAAYLMAPPGQSRRIDPAPSC"
     gene            3320..5029
                     /gene="ilvB"
                     /locus_tag="VFW59_09245"
     CDS             3320..5029
                     /gene="ilvB"
                     /locus_tag="VFW59_09245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX5364443.1"
                     /translation="MELTGAEIVIRCLQEEQVEYVFGYPGGAVLFIYDALFQQDKVKH
                     ILVRHEQAALHAADGYARSSDKVGVALVTSGPGLTNAVTGIATAYMDSIPMVIISGQV
                     PTYAIGEDAFQEVDAVGITRPCVKHNFLVKDPKDIAATIKKAFYLAKTGRPGPVLVDI
                     PKDVSNQKAIFDYPSSVSIRSYHPVGKGDSMQLRQAAQMLLEAKRPVIYAGGGVVQGD
                     ASQQLIELAHLLGAPCTNTLMGLGGFPASDKQFVGMLGMHGTYEANMAMQNCDVLLAV
                     GARFDDRVIGNVAHFAEVPRKIIHIDIDPSSIAKRVKVDLPIVGDLKLSLDELLGVLR
                     NSKRKPDAADMTAWWKQVDEWRVKGGGLRYRNSTEVIKPQYVIETLHKITGGDAFVAS
                     DVGQHQMWAAQYYKFDKPRRWINSGGLGTMGFGLPAAMGVQLINPGATVACVTGEGSI
                     QMNIQELSTCKQFRLPIKVVALNNRYLGMVRQWQEFFHGNRYSESYMDALPDFVKLAE
                     AYGHVGMRIEKPSDVEPALKEAFARKNDLVFMDFQTDPTENVFPMVPGGKGLSEVILA
                     EDL"
     gene            5029..5520
                     /gene="ilvN"
                     /locus_tag="VFW59_09250"
     CDS             5029..5520
                     /gene="ilvN"
                     /locus_tag="VFW59_09250"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX5364444.1"
                     /translation="MRHIISLLMENEAGALSRVAGLFSARGYNIESLTVAATEDPTLS
                     RMTIVTRGSDAVIEQIYKQLNKLVDVVAVMDLTDGDHLERELMLVKVRAQGEAREELK
                     RMADIFRGRIIDVSNDTYTIELTGACAKLDHFLDAIGRELILETVRTGASGIGRGDRI
                     LKL"
     gene            5540..6556
                     /gene="ilvC"
                     /locus_tag="VFW59_09255"
     CDS             5540..6556
                     /gene="ilvC"
                     /locus_tag="VFW59_09255"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5364445.1"
                     /translation="MKVYYDKDADLSLIKGKKVTIVGYGSQGHAHAQNLKDSGVNVTV
                     ALRKGGASWAKAESAGHKVAEIADAVKGADLVMILLPDESTPAVYHADVEKNLKAGAA
                     LAFAHGFNIHYNQIVPPADVDVIMIAPKGPGHTVRSEYLKGGGVPTLIAVYQDKTGKA
                     KDIALSYAAANGGTKGGVIETNFREETETDLFGEQAVLCGGAVELVKAGFETLTEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGQRVIADQARAAMKE
                     CLTNIQNGNYAKQFILEGRTNYPEMTARRRLNAEHPIEVVGGKLRAMMPWIGKNKLVD
                     QSKN"
     gene            6646..7296
                     /locus_tag="VFW59_09260"
     CDS             6646..7296
                     /locus_tag="VFW59_09260"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX5364446.1"
                     /translation="MNNYPHPIIAREGWPFLAIALAVAVLASSFAPGFLSFLAWIVAL
                     FVLQFFRDPPREIPQDVGAVLSPADGRVIKVERTQDPYGKRDAILISVFMNVFNVHSN
                     RSPVDGKVEQVQYFPGKFVNADLDKASLENERNAVVLTTNDGRTVTFVQVAGLIARRI
                     LCYIKPGDVLARGQRYGFIRFGSRVDVYLPLDASVKVSIGDKVSATTTILAVLPQS"
     gene            7325..8074
                     /gene="pssA"
                     /locus_tag="VFW59_09265"
     CDS             7325..8074
                     /gene="pssA"
                     /locus_tag="VFW59_09265"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX5364447.1"
                     /translation="MDDFTTRKPMNLRRRGIYLLPNLFTTGALFAGFYAIVQSMNGRF
                     EQAAVAIFIAMVLDGLDGRVARLTHTQSEFGAEYDSLSDMVSFGVAPALVMYEWAFKG
                     LGKWGWFAAFIYCAATALRLARFNTNIDVVDKRYFQGLPSPAAAALVAGFVWVMLDYG
                     FKGSELSWYAAALTVFAGLTMVSNLPFYSFKDFNMRKSVPFLVIFLIALFFILISSYP
                     PGVLFALFLLYSLSGYVLWLLRRGKSSQTVA"
     gene            8266..>9340
                     /locus_tag="VFW59_09270"
     CDS             8266..>9340
                     /locus_tag="VFW59_09270"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX5364448.1"
                     /translation="MTDKLIIFDTTLRDGEQSPGASMTKEEKLRIARQLEKLGVDVIE
                     AGFAAASPGDFDAVKSIAETIKNSTVCSLARASENDVRRAGEAIKPAKSGRIHTFIAT
                     SPIHMQHKLRMTEDQVVERAVQAVKWAREYTDDVEFSAEDAIRSEMDFLVRIFDAVIQ
                     AGATTINVPDTVGYSIPVLLGEHFRQLIARVPNSDKVIWSAHCHNDLGMASANSLAAV
                     MNGARQVECTINGLGERAGNAALEEIVMAVKTRRDIFTCDTRIDTAQIVPTSKLVSSI
                     TGYPIQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWGANKMVMGKHSGR
                     AAFRARLQELGIVLDGEEAVNAAF"
BASE COUNT         1832 a         2589 c         2944 g         1975 t
ORIGIN      
        1 aggctattgc gccttatggg gactagcgaa gaccaagata aatggtgatc gcaaggagaa
       61 tgagaccagc tattacgcta acaacgatgc tttcagccaa gtgcaaccac caacgttttg
      121 gctttgttgg tggtgattcg atctggatat tgctcttaat gtttgagtgt tctatttcgc
      181 cttggttttc gatcattgtt tcaccgccaa tttgcacatt cccgacgata ttggcatttg
      241 taatctttcc actgggagcg ttttttaaga gcgccataat gattaccctt gaggtgaatt
      301 aaatacaaaa attgtttaat gatccgctcg aagcattgtt gggccggaga agacgacatc
      361 cagaattcgc tgcttcaaac aactgcaagc ctgctcccaa tccctcgcaa taacaatacg
      421 ccgaacggcc tatccttttt tctgttgcca cgcttccagc cgcgcgtgca tgcgcttgta
      481 gtgcgagcca cgccagtaga ctctgccgca cttcgggcag tggagcagcg gttgatcggg
      541 gtggcgcgcg tcatccggga tgcgcgctgt cgcggcttcg tcggcttgca ccagcggggt
      601 gttgtcgatc aggcagcggg tgaaggcgtg ctccagccag tcgagatgga agcgcgcatc
      661 gaccagttcg gctaggcggt cgagcggggc gtgcggcagg atcagcgcga tgccgcaggc
      721 ggccttgtgt tcgcgcacca gcgtgtcctg tgtcaggaag tatctgcctt cctcgctgca
      781 ctggcgcagc aggtcggcgt cgctcgcgct gtcatttgct agcagcgtgt cgtaaccggc
      841 ggcgcgcagg tagcggcaca ggtggcctag catttcgtcg cagaggaagc tgggttggct
      901 catggcgcga tattaaaccg gaacggtaga cggggaagag cggcaacatt gggcggttgg
      961 gggctttgta ggagcggctt cagccgcgat tgcaatgtat tctccatacc gcctatcgcg
     1021 gctgaagccg ctcctacggg tggaatgttt cgaatgcgat ggcgggcaat ggataggccg
     1081 ccctttaaaa taatgggcga taaaattact caaccattgt tcgtcgggcg gtaattggct
     1141 agaatccggc atcccggaat ccgatccacc gtccacagga gaaataaaat ggaacatact
     1201 ctgccagctt tgccctatgc catggatgcg ctgcaaccgc acatgtccaa ggaaactttc
     1261 gagtaccact acgccaagca ccatcaggcc tatgtgacca atctgaacaa cctgatcaag
     1321 ggcaccgagt tcgagaacct cgatctcgag gccatcgtga agaaggctcc ggctggcggc
     1381 gtttacaaca acgcggcgca ggtgtggaac cacacgttct tctggaactg catgaagccg
     1441 aacggcggcg gtgcgccgac cggtgcgctg gccgatgcca tcaacaaaaa atggggcagc
     1501 gtcgatgaat tcaagaaggt ctttcaggct tccgccgtgg gcaacttcgg ttccggctgg
     1561 acctggctgg tgaagaaggc agacggctcc gtggatatcg tcaacatggg cgcggcgggt
     1621 actccgctga ccacgggcga caaggccctg ttgtgcgtgg acgtgtggga gcatgcctac
     1681 tacatcgact accgcaacat gcgtcccaag ttcgtcgaga cttacctgaa caatctggtg
     1741 aactgggact tcgtggcgaa gaatttcgcc tgagtctcgt cgctgtctgc tgcaacaaaa
     1801 gaaccgggga ttccccggtt ttttgttgcc ggcaggtcag caggacggtg cggggtctat
     1861 cctgcgcgac tggcctggcg gggccatgag gtacgcggcc tcgttcggat cgatgcccca
     1921 tttttctgcg gactcgtaac cgacgatgcg gtcgcccgat tgtgtcgcaa gatccagctc
     1981 atagggtttg atgcggcatg agcggttggt gtcgtaggtg accagcacgg cgggtttcag
     2041 ctgattggcc gggttgtgcg ttgcgacgat gcccagcagg ccgctttcca gcctgaccag
     2101 cgtgcctgtc gggtagatgc ctacgcaaca gatgaagtga tctaccagct cgacattgaa
     2161 atgctgctcg ctccattcgc gcagtttccg gatggcttcc accggttcca tggcggcgtg
     2221 atagctgcgc gcgctggtga tggcgtcata gacgtcgacg atggcggcca tctgtccata
     2281 ttgcgagagg tgatcgctct tcagcgccgc aggatagccg ccgccgtcat agcgttcgtg
     2341 gtgttgcgcg acgacctgga gtgagatggg cgtgagccac ggcgcatcgg cgagtatctc
     2401 catgccgtat tcgacatggc gttttatttc gtcgaattcg gcgtcggtca gtcgtcccgg
     2461 cttgtcgagg atggactccg cgatgcgggt tttgccgatg tcgtgcagca tggcgccgat
     2521 ggcgagttgg cgggtgatgt cgcgcccgat gccgagttgc tgggcgaagg cgctcagcag
     2581 cgagcaggtc gcgacggaat ggtagaacag atactggttg gcgttgcgta tggtgcccag
     2641 atgcagcagc gggtcggcat tccggaagat cgagtcgacg atgccctgta cggcggggcg
     2701 tacctgttcc agttcggcat gtttgcccag acgcgtcgtg gtcatgagtt catgcaccgt
     2761 caggctggct tcttcgtaca cctgatgggc caggtcgata tccgaggcgg agagcggcgc
     2821 cgggtgcgtg tcggccgagg cgttgcgttg cgccatgttc agcagcgtct ggtcgatgtc
     2881 gtcgcgcact tcctgtgcgg tcggtgcatc gggcgcatcg cggcccagcc gggtatcgat
     2941 gtatacggag gagacgccgc gctcgatcag gcggtcgatc gcccgagtgt cgtcgagatg
     3001 gaattgggtg tgcagcaatg gatgaaagac atggccgcaa ttgaggtcat ggatgaacat
     3061 gcccggctgc aattcgtcga cccttacttt cttgatcatg cgtcttcact ccatggggtg
     3121 agccagattt accgggggcg tatggcaagg catcgtcatt atgcgtcgcg caagccaagc
     3181 tcgccagtcc tgtcgcaggg gctgctgtcg ccctgaaaaa tcgcccattc tgacaatagg
     3241 gacgcttctg aggttgacca aaacgccgct aacctttatc ttacgcggtt ctttttggct
     3301 taaatttaag gtgttcgtga tggagttgac cggcgcggaa attgttatcc gctgtttgca
     3361 ggaggagcag gtcgaatacg tgtttggcta tcccggcggt gcagtgctgt tcatttatga
     3421 cgcgctgttc cagcaggata aggtcaagca tattctggtg cgtcacgaac aggcggcgct
     3481 gcatgctgcc gacggttatg cgcgttcgtc cgacaaggtc ggcgtggcgc tggtgacctc
     3541 gggtcccggc ttgaccaatg ccgtgaccgg tatcgcgacg gcctatatgg actcgatccc
     3601 gatggtcatc atcagcgggc aggtgccgac ttacgcgatc ggcgaggacg cctttcagga
     3661 agtcgatgcc gtgggcatca cccgtccctg cgtgaagcat aacttcctgg tcaaggaccc
     3721 gaaggacatc gccgcgacca tcaagaaggc gttctatctg gccaagaccg gacgtcccgg
     3781 ccccgtgctg gtggatatcc ccaaggatgt gtccaatcag aaggcgatct tcgactatcc
     3841 gtccagtgtg agcatccgtt cctatcatcc ggtcggcaag ggcgacagca tgcagctccg
     3901 ccaggcggcg cagatgctgc tggaagccaa gcgtccggtg atctatgccg gcggcggcgt
     3961 ggtgcagggc gatgcctcgc agcagttgat cgagctggcg catctgctcg gtgcgccctg
     4021 caccaacacc ctgatggggc tgggcggctt ccctgccagc gacaagcagt tcgtcggcat
     4081 gctggggatg cacggcacct acgaggccaa catggcgatg cagaattgcg acgtgttgct
     4141 ggcggtgggc gcgcgtttcg acgaccgcgt gatcggcaac gtggctcatt ttgccgaagt
     4201 gccgcgcaag atcatccata tcgatatcga cccgtcctcg atcgccaagc gcgtcaaggt
     4261 ggatctgcct atcgtcggcg acctcaagct gtcgctggac gagttgctgg gcgtgttgcg
     4321 caacagcaag cgcaagccgg atgcggcaga tatgaccgca tggtggaaac aggtggacga
     4381 gtggcgcgtc aagggcggcg ggctgcgtta ccggaattcg accgaggtca tcaagcccca
     4441 gtacgtgatc gagacgctgc acaaaatcac cggcggcgat gccttcgtgg cttccgacgt
     4501 ggggcagcac cagatgtggg cggcgcagta ctacaagttc gacaagccgc gccgctggat
     4561 caattccggc ggtctgggca cgatgggttt cggtctgccc gccgcgatgg gcgtgcagct
     4621 gatcaatccg ggcgcgaccg tggcctgcgt gaccggcgag ggcagcatcc agatgaatat
     4681 ccaggagctg tcgacctgca agcagttccg tctgccgatc aaggtggtgg cgctgaacaa
     4741 ccgttatctg ggcatggtgc gccagtggca ggagttcttc cacggcaacc gttattccga
     4801 gtcttacatg gatgcgctgc ctgacttcgt caagctggcc gaggcttacg gccacgtcgg
     4861 catgcgcatc gagaaacctt ccgacgtcga accggcgctg aaagaggcgt tcgcgcgcaa
     4921 gaacgatctg gtgttcatgg acttccagac cgatccgacc gagaacgtgt tcccgatggt
     4981 gccgggcggc aagggcttgt ccgaagtgat tctggcggag gacctgtgat gagacatatc
     5041 atttccctgc tgatggagaa tgaagcgggc gcgttgtcgc gtgtggccgg attattctcg
     5101 gcgcgcggtt ataatatcga gtcgctgacc gtggctgcca ccgaagatcc gacgctgtcg
     5161 cgcatgacca ttgtcacgcg cggctccgat gcggtgatcg aacagatcta caagcagctg
     5221 aataaactgg tggatgtggt cgcggtgatg gatctgaccg acggcgatca tctggagcgc
     5281 gagctgatgc tggtcaaggt cagggcgcag ggtgaagcgc gcgaggagct gaagcgcatg
     5341 gcggatattt tccgtgggcg catcatcgac gtgtcgaacg atacctacac catcgaattg
     5401 accggggctt gtgcaaaact tgaccatttc ctggacgcga tcggtcgcga gctgattctg
     5461 gaaacggtgc gtaccggtgc ttcgggcatc ggtcgcggcg atagaatttt aaaactttaa
     5521 ttttaacgaa gagggcaaca tgaaagttta ttacgacaaa gacgcagacc tttccctgat
     5581 caagggcaag aaagtaacca tcgtgggtta cggctcgcag ggccacgccc acgcgcagaa
     5641 cctgaaggat tccggcgtca atgtgaccgt tgcgttgcgc aaaggcggcg cttcctgggc
     5701 caaggccgag tccgccgggc acaaggttgc cgagatcgcc gacgccgtga agggcgccga
     5761 tctggtgatg atcctgctgc cggacgaatc gactcctgcc gtgtatcacg ccgacgttga
     5821 gaaaaacctg aaggctggcg cagcgctggc attcgctcac ggtttcaaca tccattacaa
     5881 ccagatcgtg ccgcccgcag acgtggacgt gatcatgatc gcccctaaag gcccgggcca
     5941 caccgtgcgt tccgaatacc tcaagggcgg cggcgttcct acgctgatcg cggtgtatca
     6001 ggacaagacc ggcaaggcga aggacatcgc gttgtcctat gcggctgcca acggcggcac
     6061 caagggcggc gtgatcgaga ctaacttccg cgaagagacc gagaccgatc tgttcggcga
     6121 gcaggccgta ttgtgcggcg gcgcggttga actggtgaag gctggcttcg agaccctgac
     6181 cgaggcgggc tatgcgcctg agatggctta cttcgagtgc ctgcatgaac tgaaactgat
     6241 cgtcgacctg atgtacgagg gcggcatcgc caacatgaac tactccattt ccaacaacgc
     6301 ggaatacggc gagtacgtca ccggccagcg cgtgatcgcc gatcaggcgc gtgccgcaat
     6361 gaaggaatgt ctgaccaaca tccagaacgg caactatgcc aagcagttca tcctggaagg
     6421 ccgtaccaac tatcccgaga tgactgcccg tcgccgtctg aacgcggagc atccgatcga
     6481 agtcgtaggc ggcaagctgc gcgcgatgat gccttggatc ggcaagaaca agctggtcga
     6541 tcaatcgaag aactaatatc cgggtgacgg aagaaggggg aagggaaaag ggtaagcgca
     6601 tcaccctcct tccccctttt ttcttgtcta cggtacctat cccaaatgaa taactatccc
     6661 caccccatca ttgcccgcga aggctggccc ttcctggcga tcgctcttgc agtcgccgtg
     6721 ctggcctcca gttttgcacc gggtttcctg tcttttctgg cttggatcgt cgccctgttc
     6781 gtgttgcagt tctttcgcga tccgccgcgc gagattccgc aggatgtcgg tgcggtgttg
     6841 tctcctgccg atggccgcgt catcaaggtc gagcgtacgc aggaccctta cggcaagcgt
     6901 gacgctatcc tgatcagcgt gttcatgaac gtgttcaacg tgcactccaa ccgcagtccg
     6961 gtggatggca aggtggaaca ggtgcagtac ttccccggca agttcgtgaa tgccgatctg
     7021 gacaaggcat cgctggagaa cgagcgcaac gccgtggttc tgacgaccaa cgacggtcgc
     7081 accgtgacct tcgttcaggt ggcggggctg atcgcccggc gcatcctgtg ctacatcaag
     7141 cccggcgacg tgttggcgcg cggccagcgt tacggtttca tccgtttcgg ctcaagggtg
     7201 gatgtctatc tgccgctgga tgccagcgtg aaggtcagta tcggcgacaa ggtctcggcg
     7261 acgacgacta ttctggccgt gcttccgcaa agctgatcgc gccgatttaa tggataatgg
     7321 gcacatggac gacttcacta cacgtaaacc gatgaacctg cgcaggcgcg gcatctatct
     7381 gttgcctaac ctgttcacga ccggcgcgct gttcgccggg ttttatgcca tcgtgcagtc
     7441 gatgaacggg cgtttcgagc aggcggcggt ggcgatcttc atcgccatgg tgctggatgg
     7501 tctggatggc cgcgttgcca ggctgacgca cacgcagagc gaattcggtg ccgagtacga
     7561 cagcctgtcg gacatggtct cgttcggggt cgcccccgcg ctggtgatgt acgaatgggc
     7621 attcaagggg ctgggtaagt ggggctggtt cgccgccttc atctactgtg ccgcgaccgc
     7681 cttgcgtctg gcccgtttca ataccaacat cgatgtggtc gacaagcgtt atttccaggg
     7741 cttgcccagc ccggccgcag cggcattggt ggcgggtttc gtctgggtga tgctggatta
     7801 cggtttcaag ggcagcgaac tgagttggta tgcggccgcg ttgacggtat ttgccggact
     7861 gaccatggtc agcaatctgc cgttctacag cttcaaggat ttcaacatgc gcaagagcgt
     7921 gccgtttctg gtgatctttc tgatcgcgct gttcttcatc ctgatctcca gctatccgcc
     7981 cggtgtcttg ttcgcgctgt tcctgctgta ttcgttgtcc ggatatgtgc tgtggttgct
     8041 ccgtcggggc aaaagttcgc agaccgttgc gtgaggcgtc aaagctggta atattcgccc
     8101 atgcacttct ctgcccacat cgcacaacta cactcgacgt tcagcctgct ggctgcgctg
     8161 ctgctgcgcg gcgcgcgcaa ataactcacc cacaatccga agtagtgcac gcgacagcca
     8221 aggacggcgt cgcaggttaa atctatccgt gcaggagtct agaccatgac agacaaattg
     8281 attattttcg ataccacctt gcgtgacggc gagcagagtc cgggcgcttc gatgaccaag
     8341 gaagaaaagt tgcgcatcgc ccgccagctg gagaagctgg gtgtggacgt gatcgaggcg
     8401 ggctttgccg ccgcaagtcc cggcgacttc gatgcggtga agtcgatcgc cgaaacgatc
     8461 aagaattcca ccgtgtgttc gctggcgcgc gccagcgaga acgatgtgcg ccgtgccggc
     8521 gaggcgatca agcccgccaa gtcgggacgc atccatacct tcatcgcgac cagcccgatc
     8581 cacatgcagc acaagctgcg catgaccgag gatcaggtgg tggagcgcgc ggtgcaggcg
     8641 gtgaagtggg cgcgcgaata taccgacgat gtcgaatttt ccgccgaaga tgcgatacgt
     8701 tccgagatgg attttctggt gcgtatcttc gacgccgtca ttcaggccgg tgcgaccacc
     8761 atcaacgtcc cggataccgt gggctacagc attccggtgt tgttgggcga gcatttcagg
     8821 cagttgattg cgcgcgtacc gaattcggac aaggtgatct ggtctgcgca ttgccacaac
     8881 gatttgggta tggcgtcggc caactcgctg gctgcggtga tgaacggcgc gcgccaggtc
     8941 gaatgcacca tcaacgggct gggcgagcgc gcgggcaatg cggcgctcga agagattgtg
     9001 atggcggtga agacgcgccg cgacatcttc acctgcgata cgcgcatcga caccgcacag
     9061 atcgtgccga cctccaagct ggtgtccagc atcaccggtt acccgatcca gccgaacaag
     9121 gccatcgtcg gcgcgaatgc cttcgcgcac gagtccggca tccatcagga cggcgtgctt
     9181 aagcaccgcg agacttacga gatcatgcgt gccgaggatg tgggctgggg cgcgaacaaa
     9241 atggtgatgg gcaaacactc cggtcgtgcc gccttccgcg cgcgcttgca ggaactgggc
     9301 atcgtgctgg acggcgaaga agccgtgaac gccgcctttg
//
LOCUS       DASUIV010000257         3671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_439277, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000257 DASUIV010000000
VERSION     DASUIV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439277"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /gene="ftsH"
                     /locus_tag="VFW59_09275"
     CDS             <1..1346
                     /gene="ftsH"
                     /locus_tag="VFW59_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5364449.1"
                     /translation="HIPKGVLMVGSPGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFV
                     GVGAARVRDMFEQAKKHAPCIIFIDEIDAVGRQRGAGMGGGNDEREQTLNALLVEMDG
                     FEGASGVIVIAATNRPDVLDSALLRPGRFDRQVVVPLPDIRGREQILKVHMRKVPVAP
                     DVQGDILARGTPGMSGADLANLVNEAALFAARRGKRFVDMDDFESAKDKIMMGAERKS
                     LIMPEEERRNTAYHESGHAVVAKLLPKTDPVHKVTIIPRGRALGLTMQLPSEDRYSMD
                     KNRILDTLAVLFGGRIAEEIFMNQMTTGASNDFERATDMARKMVTQWGMSDALGPMVY
                     GENDGEVFLGRSVTTHKNVSEATMQKVDEEIRRIIDQQYALARSLIEANRDKIEAMTK
                     ALLEWETIDADQINDIMAGNEPRPPKPTQSNKAPEPPKDPTATEPAATNQPAEGV"
     gene            1381..2202
                     /gene="folP"
                     /locus_tag="VFW59_09280"
     CDS             1381..2202
                     /gene="folP"
                     /locus_tag="VFW59_09280"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5364450.1"
                     /translation="MLHCKDFRFDLSRPLVMGIVNVTPDSFSDGGLHLRHEAAYAHAQ
                     KLIADGADILDIGGESTRPGAPQVSVQEELDRVLPLIERLRGSPVPISIDTCKPEVMR
                     AAIAAGVQMVNDINALQDTAALQAVSASEVAVCLMHKQGTPQTMQRQPDYQDVVAEVH
                     DFLRQRIAAAEAAGIARERIAIDPGFGFGKTLTHNLELLRHQDRLRSLGVPILAGLSR
                     KSMLGAITGREVGERMAASIAAALIAVQRGASIVRVHDVRETVDALKILNAVNGE"
     gene            2204..3553
                     /gene="glmM"
                     /locus_tag="VFW59_09285"
     CDS             2204..3553
                     /gene="glmM"
                     /locus_tag="VFW59_09285"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX5364451.1"
                     /translation="MSRKYFGTDGVRGRVGDHPITPQFVMHLGYAAGKVLASAEHTRG
                     ERPAVLIGKDTRISGYMLESALEAGLIAAGIDVYLAGPVPTPAVAYLTRALRLQAGVV
                     ISASHNPFEDNGIKFFSADGTKLPDETERAIEAELDNEMQTNASESLGKAKRIDDAAG
                     RYIEFCKSTFPSDMDLRGMKIVVDCAHGAAYHIAPHVLHELGADVVAIGAKPDGKNIN
                     DGFGATKPANLQKAVVENKADLGIALDGDGDRLVMVDANGVLYDGDQLIYVIARHRQA
                     QGRMQGGVVGTLMTNLAFEHAMQKLGIPFARAKVGDRYVMEQLLANGWQLGGENSGHV
                     ICLDKHSTGDGIVSALQVLHALRASGQSLAEASAGLRMYPQVLINVRVAKGTNCLGHA
                     DVLAAVAEAERELDGKGRVLLRPSGTEPLLRVMVEGEDGELVKRCAERIAEVVRCVA"
BASE COUNT          700 a         1091 c         1244 g          636 t
ORIGIN      
        1 gacatattcc gaagggcgtc ctgatggttg gttcgcctgg tacgggtaag acgctgctcg
       61 ccaaggctat tgccggcgag gccaaggtgc cgttcttctc gatttccggc tcggatttcg
      121 tcgagatgtt cgttggcgtc ggtgcagcgc gtgtgcgcga catgttcgag caggccaaga
      181 agcacgcccc ctgcatcatc ttcatcgacg agatcgacgc ggtcggtcgt cagcgcggcg
      241 caggcatggg tggcggcaac gacgagcgcg agcagaccct gaatgccttg ctggtggaaa
      301 tggacggttt cgagggtgct tccggcgtga tcgtgatcgc cgcgaccaac cgtcccgacg
      361 tgctggattc cgcgctgctg cgtcccggtc gtttcgaccg tcaggtggtc gtgccgctgc
      421 cggatattcg cggccgcgag cagatcctca aggtgcatat gcgcaaggtg ccggtggcac
      481 ccgatgtgca gggcgatatt ctggcgcgcg gcacaccggg catgtccggt gccgatctgg
      541 ccaatctggt gaacgaagcg gcgctgttcg cggcgcgtcg cggcaagcgt ttcgtcgaca
      601 tggacgattt cgaatcggcc aaggacaaga tcatgatggg cgccgagcgc aaatccctga
      661 tcatgccgga agaagagcgc cgcaacaccg cgtatcacga gtccggtcat gccgtggtgg
      721 ccaagctgct gcccaagacc gatccggtgc acaaggtcac catcatcccg cgcggccgcg
      781 cgctgggtct gaccatgcag ttgccgtcgg aagaccgcta cagcatggac aagaaccgca
      841 ttctggacac gctggccgtg ttgttcggcg ggcgtatcgc cgaagagatt ttcatgaacc
      901 agatgaccac gggcgcttcc aacgacttcg agcgcgccac cgacatggcg cgcaagatgg
      961 tcacccagtg gggcatgtcc gatgcgctgg gcccgatggt gtatggcgag aacgacggcg
     1021 aggtgttcct cggccgttcg gtgaccacgc acaagaatgt gtccgaagcc accatgcaaa
     1081 aagtggacga ggaaattcgc cgcattatcg accagcaata tgcgctggcc cgcagcctga
     1141 tcgaagccaa tcgcgacaag atcgaggcga tgaccaaggc cttgctggag tgggagacca
     1201 tcgacgcgga tcagatcaac gacatcatgg ccggcaacga gccgcgtccg ccgaagccta
     1261 cccagagcaa caaggcgccg gagccgccga aggatccgac cgcgacggag cccgcagcga
     1321 ccaaccagcc tgcggaaggc gtttaactcg acacacgggg gctgcggccc ccgttctctt
     1381 atgttgcatt gcaaagattt tcgattcgat ctttcccgtc cgctggtgat ggggatcgtc
     1441 aacgtcacac cggattcctt ctctgacggc gggctgcatc tgcggcatga ggctgcatat
     1501 gctcacgcgc agaaactgat cgcagacggc gcggacatcc tcgacatcgg cggcgaatcc
     1561 acccgtcccg gcgcgccgca agtcagcgtg caggaggagc tggaccgggt gctgcccctc
     1621 atcgagcgcc tgcgcggcag tcccgtcccg atctccatcg acacctgcaa gcctgaagtg
     1681 atgcgcgcgg ccatcgcggc gggtgtgcag atggtcaacg acatcaatgc actgcaagac
     1741 acggcggcac tgcaggcagt ctccgccagc gaggtcgcgg tgtgcctgat gcacaagcag
     1801 ggcacgccgc agaccatgca gcggcagccg gactatcagg atgtcgtcgc cgaggtgcat
     1861 gattttctgc gccagcgcat cgctgccgca gaagcggccg ggatcgcgcg cgagcgcatc
     1921 gcaatcgatc cgggctttgg tttcggcaag acgctgacgc ataatctcga actgctgcgt
     1981 catcaggaca ggctgcgcag tctgggcgtg ccgatactcg cgggcctgtc gcgcaagtcc
     2041 atgctgggcg cgatcaccgg gcgggaggtg ggcgagcgca tggccgccag tatcgcggcg
     2101 gcgctgatcg cggtgcaacg cggtgcttcc atcgtgcgcg tgcatgatgt gcgcgagacg
     2161 gtggatgcgc tgaaaatatt aaatgcagtg aacggggaat gagatgagca gaaaatattt
     2221 cggtacggac ggtgtgcgcg ggcgcgtggg cgatcatccc atcacgccgc aattcgtgat
     2281 gcatctgggt tatgcggcgg gcaaggtgct ggccagcgcg gagcatacgc gcggcgaacg
     2341 gcccgccgtg ctgatcggca aggacactcg catctccggc tacatgctgg aatccgcgct
     2401 cgaggccggg ctgatcgcgg cgggtatcga cgtgtatctg gcagggccgg tgccgacgcc
     2461 cgccgtggcc tatctgaccc gcgcgctgcg cctgcaggcg ggcgtggtca tctcggcctc
     2521 gcataatccc ttcgaagaca acggcatcaa gttcttctcc gccgacggca ccaaactgcc
     2581 ggacgagacc gagcgcgcca tcgaggccga gctggacaac gagatgcaaa ccaatgcctc
     2641 ggaatcgctg ggcaaggcca agcgcatcga cgatgcggcg ggacgctaca tcgagttttg
     2701 caagagcacc ttcccgtccg atatggatct gcgcggcatg aagatcgtgg tggactgcgc
     2761 gcacggcgcg gcctatcaca tcgcgcccca tgtgctgcac gagctgggtg cggacgtggt
     2821 cgccatcgga gcgaagccgg acggcaagaa catcaacgac ggcttcggtg cgaccaagcc
     2881 tgcgaatctg cagaaggccg tggtcgagaa caaggccgac ctcggcatcg cgctggacgg
     2941 cgacggcgac cgtctggtga tggtggatgc gaacggcgtg ctgtacgacg gcgaccagtt
     3001 gatctatgtg atcgccagac atcgtcaggc gcaaggcagg atgcagggcg gcgtggtcgg
     3061 cacgctgatg accaacctcg cgttcgagca cgcgatgcag aagctcggca tcccgttcgc
     3121 gcgcgccaag gtaggcgacc gttacgtgat ggagcagttg ctcgccaacg gctggcagct
     3181 cggcggcgag aactccggtc atgtcatttg tctggacaag cacagcaccg gcgacggtat
     3241 cgtctccgcg ctgcaggtgc tgcacgcgct gcgcgccagc ggccagagcc tggcggaggc
     3301 ctcagccggc ctgcgcatgt atccgcaggt gctgatcaac gtgcgcgtcg ccaaggggac
     3361 gaattgtctc ggtcatgccg atgtgctggc ggcagtggcg gaagccgagc gcgagctgga
     3421 cggcaagggc cgcgtgctgc tgcgtccgtc cggcaccgag ccgttgttga gggtgatggt
     3481 cgagggcgag gatggggagc tggttaagcg gtgtgcggag aggattgcgg aagtggtgcg
     3541 gtgcgtggct taacgagtta agtcaccagc ctgtgcggtt ttgcgcgcag atttggtgga
     3601 ctgcagggtt agcccgccat gcttctcgta caacgtggca ataaacttaa aaagttgttc
     3661 aatcaaccaa g
//
LOCUS       DASUIV010000258         7398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_66418, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000258 DASUIV010000000
VERSION     DASUIV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66418"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFW59_09290"
     CDS             <1..870
                     /locus_tag="VFW59_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5364452.1"
                     /translation="PDIELEVFIHGALCIAYSGRCLLSGYFNHRDANQGTCTNSCRWD
                     YKVENAAEDGTGHIEKIDFDFDKELSESALSDCGSQPRHPLADKVYVISRQDHPGELM
                     PVLEDEHGTYIMNSKDLRAVEHVERLVKIGIDSLKVEGRTKSIYYVARTAQVYRQAID
                     DAVAGRPFNLSLLGALDALANRGYTDGFYERHHTHEQQNYMRGHSESTRQQYVGDIVS
                     CANGMAEIDVKNKFQVGDRLEIIHPSGNQIVELSEMRSLDGAPVTVAPGSGHRVMIPL
                     QGDWSQGMVARFL"
     gene            885..1610
                     /locus_tag="VFW59_09295"
     CDS             885..1610
                     /locus_tag="VFW59_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEX5364453.1"
                     /translation="MQTPAQFTRTHILLGDAGIAKLRGMHIFVAGMGGVGSYCTEALA
                     RAGIGRLTLLDHDEVVASNINRQLPALLSTIGQSKAELMRARIADINPDCQVTLLRTF
                     LSTDNVNELVPDDCDYVIDCIDSLTCKEALVAESFKRGLKVASSMGAGNRLDPTRIKI
                     GDISKTEMCPLAKQMRMRLRKHHGIRKGILTVYSDESPSAPLPPEATSRGRPRAVNGT
                     ISYMPPLFGFMLAGAVLKRLIEE"
     gene            complement(1588..2037)
                     /locus_tag="VFW59_09300"
     CDS             complement(1588..2037)
                     /locus_tag="VFW59_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HEX5364454.1"
                     /translation="MRIWSLHPGLLDAKGLVALWRETLLAQKVLAGNTRGYRNHPQLD
                     RFKCQADPLAAIGSYLREVQQEAERRGYRFDASKILCHTEVPRISVTDGQLAYELSHL
                     RNKLKSRDEPAYERISTLHSPQPHPLFDVIHGGVEHWEVVTTPRSAA"
     gene            complement(2040..3002)
                     /gene="corA"
                     /locus_tag="VFW59_09305"
     CDS             complement(2040..3002)
                     /gene="corA"
                     /locus_tag="VFW59_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX5364455.1"
                     /translation="MLQAFTLNNGRLSRIHIEEDDDLRTDQPIWIDAVSPSEEERQWL
                     ERTYQLNLPKPEHLRDIESSARFYEEGGELHLRSDFLYGKESGSRSVTVAFVLTGTTL
                     ISVHAEDLPLFRLMRIRSHGQAGEVEDSRDVLIDLYAMDVELSADALEEVYADLGAVS
                     SRVLGDKALSDREAAEVLASIAHGEHINGRIRRNVMDTRRAISFLLRSKRLSPTQVDE
                     AHQIVQDIDSLDGHTAFLFDKINFLMDATVGFININQNKIVRLFSVASVALLPPTLIA
                     SIYGMNFRSMPELSWQLGYPLALLLMALSVTIPFWIFHRKGWLK"
     gene            complement(3007..3405)
                     /locus_tag="VFW59_09310"
     CDS             complement(3007..3405)
                     /locus_tag="VFW59_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEX5364456.1"
                     /translation="MPREPEVLTHYQRIGGEQKVRELVARFYELMDELPEAYGIRKMH
                     GEELNPVADKLFKFLSGWMGGPQLFVEQYGHPMLRRRHLPFAIGESERDQWLMCMDQA
                     LNDVVTDEALRRELSTAFAKVADHMRNRTE"
     gene            complement(3449..3661)
                     /locus_tag="VFW59_09315"
     CDS             complement(3449..3661)
                     /locus_tag="VFW59_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364457.1"
                     /translation="MKQAKQRNGKQEHTVCGNCARTIKVRHDSDNLVCLAHLHFVPTS
                     HDADCEDFKPHAASEKPPSSIAKIEP"
     gene            complement(3740..4297)
                     /gene="thpR"
                     /locus_tag="VFW59_09320"
     CDS             complement(3740..4297)
                     /gene="thpR"
                     /locus_tag="VFW59_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5364458.1"
                     /translation="MTKSELFSSARVFFALWPTEAERAALANWQPPLHELCGGRTMRA
                     DTLHATLIFLGEIAEHRLEALQLAAQEVAGASFELRLDKVRYWGHNHIAYAAPSVVPA
                     PLSALVQDLEQRLRAHRFRFERREYKPHVTLLRHGRWTDAPLPEMNAVNWQVQDFALV
                     SSQPDEQGARYEVLARFPLLRSQVI"
     gene            4384..>7398
                     /locus_tag="VFW59_09325"
     CDS             4384..>7398
                     /locus_tag="VFW59_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HEX5364459.1"
                     /translation="MTARLREIPYNYTSFSDREIVLRILGQEAWDIINTLREERRTGR
                     SAQMLYEVLGDIWVLTRNPYLEDDLLANRKRREALIGALHHRLNAIEERRQNNPKVKR
                     LLELATDAVNQFAACFEHTLQLRRRALRVLSRITHKDNIRFDGLSRVSHVTDATDWRV
                     EYPFVVLNPDSEEEIAHLVRACIELELTIIPRGGGTGYTGGAVPLDKHSAVINTEKLD
                     ALSPVEQLTLPGLSRPYATVHCGAGVVTRRVMEAAEACDLVFAVDPTSADASCIGGNV
                     AMNAGGKKAVLWGTALDNLASWKMVTPDGLWMEVERIDHNLGKIHDAEFANFRISRFE
                     ADGKTPHGESEMLNIPGSAFRKAGLGKDVTDKFLSGLPGIQKEGCDGIITSARFILHR
                     AHAHTRTVCLEFFGQVREAVPAIIEITTLLNNRTGSPVFLAGLEHLDERYLKAVGYAT
                     KSKRGIRPKMVLVADVVSDDADAAAQAASEIVRLANLRSGEGFIAVSAEARKKFWLDR
                     ARTAAIAKHTNAFKINEDVVIPLPRMGDYCDGVERINIELSLKNKLKLLDALDRFFAT
                     ELPLRYQDDAQLGDAELLGNRPQEARELLSGVRARWQWLLDNLDAPLGEEILAAKDRQ
                     DAVTVFDAVQRHMLRASWKRELREPLRHLFSGSTYQPVLEQCNTIHQQVLKSRVFVAL
                     HMHAGDGNVHTNIPVNSDDYAMLQQANGAVDRIMQLAKDLGGVISGEHGIGITKFDFL
                     EPQEIAAFTAYKQKVDPEGRFNKGKLLKGGNLEHAYTPSFNLMELESLILEKSELSEI
                     SDSIKDCLRCGKCKPVCSTHVPRANLLYSPRNKILATSLLIEAFLYEEQTRRGISIAH
                     FDEFGDVADHCTVCHRCLKPCPVDIDFGDVSMAMRNFLRKQGKKKFNPGTAAAMTFLN
                     ATDPATIKLIRKFLIEWSYKGQRLGHKLAKRFGLLGAQTKRPPLTLGRAPIKAQVIHI
                     LNKPLPANVPSKTSRALLDIEDSSVVPIIRDPR"
BASE COUNT         1395 a         2282 c         2323 g         1398 t
ORIGIN      
        1 ccggacatcg agctcgaggt gttcatccac ggcgcgctgt gtatcgccta ttccgggcgc
       61 tgcctgttgt cgggttactt caaccaccgc gacgcgaatc agggcacctg caccaattcc
      121 tgccgctggg attacaaggt ggagaatgcc gctgaagacg gcaccggaca catcgaaaag
      181 atcgactttg atttcgacaa ggagttgagc gagagcgcgt tgtccgactg cggttcgcag
      241 ccgcgccatc cgctggccga caaggtgtac gtcatctcgc ggcaggatca tccgggcgag
      301 ctgatgccgg tgctggaaga cgagcacggc acctacatca tgaactccaa ggacctgcgc
      361 gccgtggagc acgtcgagcg tctggtgaag atcggcatcg actcgctcaa ggtcgagggc
      421 cgcaccaagt cgatctacta cgtggctcgt accgcgcagg tctatcgtca ggccatcgac
      481 gatgcggtcg cagggcggcc cttcaacctg agtctgctgg gggcgctgga tgcgctggcg
      541 aaccgcggtt ataccgacgg tttctacgaa cgccatcaca cccacgagca gcagaactac
      601 atgcgcggcc actccgagag cacccgtcag caatatgtcg gggacatcgt gagctgcgcg
      661 aacggcatgg ccgagatcga cgtgaagaat aaatttcagg tcggcgaccg gctcgagatc
      721 atccatccgt cgggcaacca gatcgtcgag ctgagcgaga tgcgcagtct cgacggcgct
      781 cctgtaaccg tcgcgcccgg cagcggtcat cgtgtgatga tcccgttgca gggtgactgg
      841 tcgcagggca tggtggcgcg ctttctgtga aggacaatcc agccatgcaa actcccgcac
      901 aatttacccg cacccacatc ctgctcggcg acgcaggcat cgcgaagctg cgcggcatgc
      961 atatcttcgt cgcaggcatg ggcggtgtcg gttcctattg caccgaggcg ctggcgcgcg
     1021 cgggcatcgg gcggctgacg ctgctcgacc atgacgaggt cgtcgccagc aacatcaatc
     1081 gccagctgcc cgcgttgctg tccaccatcg gtcagtccaa ggcggagctg atgcgcgcgc
     1141 gcatcgccga catcaacccg gattgtcagg tcacgctgct gcgcaccttt ctcagcacgg
     1201 ataacgtcaa cgaactggtg ccggacgact gcgactatgt gatcgactgc atcgattcgc
     1261 tgacctgcaa ggaggcgctg gtggcagaga gtttcaagcg cggcctgaag gtcgcctcca
     1321 gcatgggcgc gggcaaccgc ctcgatccga cgcgcatcaa gatcggcgac atcagcaaga
     1381 ccgagatgtg cccgctggcc aagcagatgc ggatgcggct gcgcaaacat cacggcatcc
     1441 gcaagggtat cctgacggtg tactcggatg aatctccgtc tgcccctttg cccccggaag
     1501 ccaccagccg cgggcgtccg cgcgcggtga acggcaccat cagctacatg ccgccgctgt
     1561 ttggcttcat gctggcaggc gcggtgctca agcggctgat cgaggagtag tcaccacttc
     1621 ccagtgttcc actcctccgt ggatcacgtc gaacaggggg tggggttgcg gcgagtggag
     1681 ggtggagatc cgctcatagg caggttcgtc gcgcgatttc agcttgttcc tgagatggct
     1741 gagttcatag gccagctgtc cgtcggtgac gctgatgcgg ggcacttcgg tgtggcacag
     1801 tatcttgctc gcatcgaaac gatagccgcg ccgttcggcc tcctgttgta cctcgcgcag
     1861 atagcttcct atcgccgcca gcggatctgc ctggcatttg aatcgatcga gttgcggatg
     1921 gttgcgatag ccccgggtgt ttcccgcgag caccttctgg gccagcaagg tctcgcgcca
     1981 caaagcgacc aaccccttgg cgtccagcag tccggggtgc aaagaccaga tgcgcatggc
     2041 tacttcagcc agcccttgcg atggaatatc cagaacggga tcgtcaccga caatgccatc
     2101 agcagcaatg ccagagggta gcccagctgc caggacagtt ccggcatgct gcggaagttc
     2161 atgccgtaga tggacgcgat cagcgtgggg ggcagcagcg ccaccgaggc gaccgagaac
     2221 aggcgcacga tcttgttctg gttgatgttg atgaagccga ccgtcgcatc catcaggaag
     2281 ttgatcttgt cgaacaggaa ggcggtgtgg ccgtcgagcg agtcgatgtc ctgaacgatc
     2341 tggtgcgcct cgtcgacctg tgtcggcgac agtcgcttgc tgcggagcag gaaggagatc
     2401 gcgcggcgcg tatccatcac gttgcggcgg atgcggccat tgatatgttc gccgtgcgcg
     2461 atgctggcca gcacctcggc tgcctcgcga tctgagagcg ccttgtcgcc cagcacccgg
     2521 ctgctgaccg caccgaggtc ggcgtacacc tcttccagcg catccgcaga cagctccaca
     2581 tccatcgcgt acaggtcgat cagcacatcg cggctgtctt cgacctcgcc cgcctgcccg
     2641 tggctgcgta tgcgcatcag ccggaacagc ggcagatctt ccgcatgcac gctgatcagc
     2701 gtggtgccgg tcagcacgaa ggccacggtc acgctgcgcg agccgctctc cttgccgtac
     2761 agaaaatccg agcgcaggtg caactcgccg ccctcctcgt agaagcgcgc gctggattcg
     2821 atgtcgcgca gatgctccgg tttgggcaga ttcagttgat aggtccgctc cagccattgc
     2881 cgttcttctt cgctgggtga gacggcgtcg atccagatcg gctggtcggt gcgcagatcg
     2941 tcgtcttcct cgatgtggat gcggctaagt cggccgttgt tgagcgtgaa tgcctgcagc
     3001 atgagactac tccgtgcggt tgcgcatatg gtcggccacc ttggcaaagg ctgtcgacag
     3061 ttcgcggcgc agtgcctcat cggtcaccac gtcgttcagt gcctgatcca tgcacatcag
     3121 ccactgatcg cgctccgatt cgccgatggc gaatggcaga tggcggcggc gcagcatggg
     3181 gtggccgtat tgctccacga atagctgcgg gccgcccatc cagccggaga ggaacttgaa
     3241 cagtttgtcg gcgacgggat ttaactcttc gccgtgcatc ttgcggatgc cgtaggcttc
     3301 cggcagttcg tccatcaatt catagaaacg cgcgaccagc tcgcgaacct tctgctcgcc
     3361 gccgatgcgt tggtaatggg tcaggacttc cggttctctt ggcatgcgat tccagatggt
     3421 gtgattggaa tgggtgaact gagcgctatc agggttcaat ctttgcgatg gacgaagggg
     3481 gtttctcgct ggcagcatgc ggcttgaaat cctcgcagtc ggcgtcatgg gaggtcggta
     3541 caaaatgcaa atgggcaagg cagaccaggt tgtcggaatc gtgcctgacc ttgatcgttc
     3601 tggcgcagtt gccgcagacg gtatgctctt gtttcccgtt acgctgcttg gcttgtttca
     3661 tctaatcccc tgaacataac tgactagcca gctggccgct tcgggcatgc cgggctctta
     3721 tattgtttgg tcggcggaat catatcacct gactgcggag gagcgggaat ctcgccagca
     3781 cctcgtagcg cgcaccctgc tcgtcgggct gagagctgac cagcgcgaag tcttgcacct
     3841 gccagttcac cgcattcatc tccggcagcg gtgcgtctgt ccatcggccg tggcgcagca
     3901 gcgtgacatg gggcttgtat tcgcgccgct cgaaacgaaa acgatgcgcg cgcagccgtt
     3961 gctccagatc ctgtaccagt gcggataatg gtgccggcac gacagaggga gcggcgtagg
     4021 cgatgtggtt gtgcccccag tagcggacct tgtccagccg cagttcgaag cttgcgcccg
     4081 cgacctcctg tgccgccagt tgcagcgcct ccagacggtg ttcggcgatc tcgccgagga
     4141 atatcagcgt ggcgtgcagc gtatcggccc gcatcgtccg tccgccgcat agctcgtgca
     4201 acgggggctg ccagttggcc agcgcggcgc gttccgcctc ggtcggccag agcgcgaaga
     4261 agacccgtgc agatgagaat aattcggatt tcgtcatggc ggcattttat ccccgttacg
     4321 cacggggtcg agggggagcc cgcattcagg taaaatgcgc gccttgtcga atatgggtag
     4381 accatgactg cacgcttgag agaaatcccc tacaactaca cctccttttc cgaccgcgag
     4441 atcgtcttgc gcatcctcgg tcaggaggcc tgggacatca tcaacacgct gcgcgaagag
     4501 cgccgtaccg ggcgttcggc gcagatgctg tacgaggtgc tgggcgacat ctgggtgctg
     4561 acgcgcaacc cttatctgga agacgacctg ctcgccaatc gcaagcggcg cgaggcgctg
     4621 atcggcgcat tgcatcaccg tctgaatgcc atcgaggagc gtcgccagaa caatcccaag
     4681 gtcaagcggt tgctggagct cgctaccgat gcggtcaacc agttcgccgc ctgtttcgag
     4741 cacaccctgc aattgcgccg ccgcgcgctg cgcgtattga gccgcatcac ccacaaggac
     4801 aacatccgtt tcgacgggct gtcgcgcgtg tcgcatgtca ccgatgccac cgactggcgc
     4861 gtggaatatc cgttcgtggt gctgaatccg gacagcgagg aagagatcgc gcatctggtg
     4921 cgcgcctgca tcgaactcga actgaccatc atcccgcgcg gcggcggtac cggctatacc
     4981 ggtggtgccg tgccgctgga caagcactct gcggtcatca acaccgagaa gctggacgcg
     5041 ctctcgcccg tcgagcagct gacgctgccc ggcctgagcc ggccctatgc cacggtgcat
     5101 tgcggcgcgg gcgtggtgac gcggcgggtg atggaagcgg cggaagcctg cgatctggtg
     5161 ttcgccgtcg accccacctc ggcggatgcc tcctgcatcg gcggaaacgt cgcgatgaac
     5221 gcgggcggca agaaggccgt gttgtggggc accgcgctgg acaacctggc ctcgtggaag
     5281 atggtcacgc cggacgggct gtggatggaa gtcgagcgta tcgatcacaa cctcggcaag
     5341 atccacgatg ccgaattcgc gaacttccgc atcagccgct tcgaggcgga cggcaagacg
     5401 ccgcacggcg aatccgagat gctgaatatt cccggcagcg cattccgcaa ggcgggtctc
     5461 ggcaaggacg tcaccgacaa gttcctgtcc ggcctgcccg gcattcagaa ggagggctgc
     5521 gacggcatca tcacctcggc gcgtttcatc ctgcaccgcg cccatgcgca tacccgcacc
     5581 gtgtgtctgg aattcttcgg tcaggtgcgc gaggccgtgc ctgcgatcat cgagatcacc
     5641 acgctgctga acaatcgcac cggttcgccg gtatttctcg ccggtctgga gcatctcgac
     5701 gagcgctatc tgaaggcggt gggttatgcc accaaatcga agcgcggcat ccgtccgaag
     5761 atggtgctgg tcgccgatgt ggtcagcgac gatgccgatg ccgccgcgca ggccgcctcc
     5821 gagatcgtgc ggctggccaa tctgcgcagc ggcgaaggct tcatcgcggt gtccgccgag
     5881 gcgcgcaaga agttctggct ggatcgcgca cgcaccgcag cgatcgccaa acataccaat
     5941 gccttcaaga tcaacgaaga tgtggtgatc ccgctgccgc gcatgggcga ctactgcgac
     6001 ggcgtcgagc gcatcaacat cgagttgtcg ctgaagaaca agctcaagtt gctggatgcg
     6061 ctggaccggt tcttcgccac cgagttgccg ctgcgctatc aggacgacgc gcagctgggc
     6121 gatgccgagt tgctgggcaa tcgtccgcag gaagcgcgtg agctgttgag cggtgtgcgc
     6181 gcgcgctggc agtggctgct ggacaatctc gatgcgccgc tgggcgaaga gatcctcgcc
     6241 gcaaaagacc ggcaggatgc cgtcaccgtt ttcgatgcgg tgcagcgtca tatgctgcgc
     6301 gcttcgtgga agcgcgagct gcgcgaaccg ctgcgccatc tgttcagcgg cagcacttac
     6361 cagccggtgc tggaacagtg caacacgatc catcagcagg tgctgaaaag ccgcgtgttc
     6421 gtcgcgttgc acatgcatgc gggcgacggc aacgtgcaca ccaacatccc ggtcaactcc
     6481 gacgattatg cgatgctgca acaggccaac ggcgcggtcg accgcatcat gcagctggcg
     6541 aaagatctgg gcggggtgat ctccggcgag catggcatcg gcatcaccaa gttcgacttt
     6601 ctcgagccgc aggagatcgc cgcattcacc gcctacaaac agaaggtcga tccggaaggg
     6661 cgcttcaaca agggcaagct gctcaagggc ggcaacctcg agcatgccta cacgcccagc
     6721 ttcaacctga tggagctgga gagcctgatc ctggagaaga gcgagttgtc cgaaatctcc
     6781 gactcgatca aggattgttt gcgttgcggc aagtgcaagc cggtgtgcag cacccatgtg
     6841 ccgcgcgcca acctgctgta ttcgccgcgc aacaagatcc tcgccacctc gctgctgatc
     6901 gaggctttcc tgtacgagga gcagacccgc agaggaatct ctatcgcgca tttcgacgag
     6961 ttcggcgatg ttgccgatca ctgcaccgtg tgccaccgtt gtctgaagcc ttgtccggtg
     7021 gacatcgact tcggcgatgt gtcgatggcg atgcgcaact tcctgcgcaa gcagggcaag
     7081 aagaaattca atcccggcac ggccgccgcg atgaccttcc tgaacgccac cgatccggcc
     7141 accatcaagc tgatccgcaa gttcctgatc gaatggagct acaaggggca gcggctgggt
     7201 cacaagctgg ccaaacgctt cggcctgctg ggcgctcaga ccaagcgtcc tccgctcacg
     7261 ctggggcgcg cgccgatcaa ggcgcaggtg atccatatcc tcaacaagcc gctgcccgcg
     7321 aacgtgccct ccaagacctc gcgcgcgctg ctcgacatcg aggacagcag cgtcgtgccg
     7381 atcattcgcg acccgcgc
//
LOCUS       DASUIV010000259         6961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_347299, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000259 DASUIV010000000
VERSION     DASUIV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347299"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VFW59_09330"
     CDS             complement(<1..1421)
                     /locus_tag="VFW59_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5364460.1"
                     /translation="MQFFWRDESLPVLVMTLGLGFLLLHFLKEERKSVLNTLTFFFAC
                     QVLQFVSGLLQGLGILSAASVLHEVGVIGAGIAVIRLWGLLVFRIVLPWARVSPPRIA
                     EDIFVIIGYVAWGLVRLRYAGLDLGSIVATSAMITAVVAFAMQDTLGNILGGLALQLD
                     NSIEIGDWIKVDDISGRVVDIRWRSTLVETRNWETVVFPNSQLMKNKFLVLGRRADQP
                     VQWRRWVWFNVGLKITPTRVISAVENAVQQAEINNVAKSPAPNCVLMEMDKGCVRYAL
                     RYWLTDLAADDPTDAAVRWHVYTALERAGIKLTVEEQNVHYIKENEKHEEALRQRETT
                     LRVKTLRRVELFAPLNDDELRKLAERLRYAPFAKGNVIAKQGTPAQHWLYIIISGDAE
                     VYLETAAGERHVLNVLTKGDIFGEMSLMTGAPRAASVLARTDVECYRLDKDAFEEVLL
                     ARPGIAEEVSQVLVSRRAALASA"
     gene            complement(1473..2477)
                     /locus_tag="VFW59_09335"
     CDS             complement(1473..2477)
                     /locus_tag="VFW59_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364461.1"
                     /translation="MKSVHLVIPDLFLPADIAAEASAGLMLPALERMLARGVSADAED
                     IGGGLEEALCGLFAPSAGTPIAAVSAAFDGLEAGHWLRADPAYMQLHRTQLVMQPAGR
                     VTAGEAAQFCAALNEHFAGQGLEFVAPHPQRWYVRLPAMPDIRTVPLSQASGRDVRGL
                     LPEGEEGPRWQQLFNEMQMLLFAHPLNELREARGEMPVNSVWLWGNGVAAIQTPCRYA
                     CVSSDEELAGMFAAAAGASFSGWTERWRAEEGEQLLVWTGLRNALQQGDLHAWRTALQ
                     SFETNHAQPLWRALRAGDIAQLQLDVPGEEGMRRIRLTRAGAWSLWRRPRRLAAHSMV
                     "
     gene            2597..3841
                     /locus_tag="VFW59_09340"
     CDS             2597..3841
                     /locus_tag="VFW59_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX5364462.1"
                     /translation="MRPYELFIGLRYTRAKRRNHFISFISLISMMGMGLGVMALIVVL
                     SVMNGFQTEIRDRMLGASPHMEVVADGGRMYDWPAVLELVEKHPQVAAAAPYVAGQGM
                     LSFGQSVQGVMVRGIDPARETAITELSNKIKAGSLDELRSGEFGIVLGSDLARALGVK
                     LNEKVMLVAPQGQITPAGMMPRLKQFRVVGIFEIGMAPYDNSLALIQIGDAQKLFLLG
                     DAVTGISSKLHDIERAPQVASELQRELPPELYTNDWTHQNSNYFRAVQMEKRMMFIIL
                     SLIVAVAAFNIVSTLVMAVTDKQADIAILRTLGAAPSSIMKIFIVQGVVIGVIGTLSG
                     CIGGIALALNLDVVVPFIEHTLGVQFLAKDVYYITELPSELRYHEVILIASMSFLISL
                     LATLYPSWRASKTQPAEALRYE"
     gene            3834..4547
                     /gene="lolD"
                     /locus_tag="VFW59_09345"
     CDS             3834..4547
                     /gene="lolD"
                     /locus_tag="VFW59_09345"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEX5364463.1"
                     /translation="MNNHRIEDQGSKIEPVISCRELSKTFTIGKQDVSVLRSVDLDVA
                     RGERLAIVGASGSGKSTLLHLLGGLDNASGGSVAILGQDVQKMNETRRGELRNRSLGF
                     VYQFHHLLPEFTALENVAMPLLIRGMRRRDAEPLAAAMLERVGLAHRIGHLPSELSGG
                     ERQRTAVARALVTEPACVLADEPTGNLDRNSAEALFELMQQLNEQFDTSFIVVTHDLD
                     LAGRMQRRLRLVDGVLQEI"
     gene            complement(4751..5281)
                     /locus_tag="VFW59_09350"
     CDS             complement(4751..5281)
                     /locus_tag="VFW59_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX5364464.1"
                     /translation="MRKFFRQFLPQHESIRNHRLFKPFSGWLHHPNLWHLHRRSVAGG
                     VAVGLFCGLIPGPFQMISALLLAVWLRVNLPLAIVVTTYTNPFTIVPLYFLAYELGLR
                     VLGASNGMLTEPPPFPAMHWDNWAHALWDWIEALGAPLMFGLPLLALGLAVAGYVLVR
                     VIWRAAVIWRWRRRQR"
     gene            5473..6378
                     /locus_tag="VFW59_09355"
     CDS             5473..6378
                     /locus_tag="VFW59_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5364465.1"
                     /translation="MLSATLKRYAYLSIAAALATILLKGIAWWMTGSVGLLSDALESF
                     VNLAGALMALAMISLAMEPADENHAYGHGKAEYFSSAFEGFLILVAAVSIGYTAIERL
                     LHPQAIESVGIGLAVSVAASIINLVTARALMAVGRKHNSITLEADAHHLLTDVWTSVG
                     VIGGVALVWLTGWLWLDPVIALLVAANIVWTGWQLLRRSASGLMDTSIPEEQIRAIET
                     VLEGYRLQGLDYHALRTRQAGMRAFITLHVLVPGSWTVQQGHDRLEQIEADIRNVVPN
                     AHLTTHLEPIEDPVSLSDQTLDRHI"
     gene            6430..6834
                     /locus_tag="VFW59_09360"
     CDS             6430..6834
                     /locus_tag="VFW59_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HEX5364466.1"
                     /translation="MTARKIGLALLLLASATPALAEKPKDFISTYSTQAGSGFTPSAA
                     RGKSFFGKDWGVSKDMPKCASCHGNDLKANGKHVITGKVIEPFSPSANPERFTSTKKV
                     EKWFKRNCKEVVGRECTAAEKADFIQFVTQGD"
BASE COUNT         1452 a         2306 c         1975 g         1228 t
ORIGIN      
        1 aatgcgctgg ccagcgcggc gcggcggctc accagcacct gcgacacttc ctccgcgata
       61 ccgggacgag ccagcaggac ttcctcgaaa gcatccttgt cgaggcggta acactcgaca
      121 tccgttctgg cgagcaccga ggcggcacgc ggcgcgccgg tcatcagact catctcgccg
      181 aagatatcgc ccttggtcag cacattaaga acatggcgct cgcccgctgc ggtctcaaga
      241 tacacctcgg catcgccgct gatgatgatg tacaaccagt gctgcgcagg cgttccctgt
      301 ttggcgatca cattaccctt ggcaaacggc gcatatctga ggcgctcggc cagcttgcgc
      361 aattcatcgt cgttcagcgg agcgaacagt tcgacgcgcc tgagcgtctt gacgcgtagc
      421 gtggtttcac gctgacgcag cgcctcttcg tgcttttcgt tttccttgat gtagtggacg
      481 ttctgttcct ctaccgtcag cttgatcccg gcccgctcca gcgccgtgta cacatgccag
      541 cgtaccgccg catcggtcgg atcgtccgcc gccagatcgg tcagccagta gcgcaaggca
      601 taacgcacgc agcccttgtc catctccatc agcacacagt tcggcgccgg gctcttggcg
      661 acattattga tttccgcctg ctgcaccgcg ttctccaccg cgctgatcac gcgggtcggc
      721 gtgatcttca gaccgacgtt gaaccacacc cagcgccgcc actgcaccgg ctgatcggca
      781 cgccgtccca gcaccagaaa cttgttcttc atcaactggc tgttcggaaa caccacggtc
      841 tcccagttgc gcgtctccac cagcgtggag cgccagcgga tgtccaccac ccgcccggag
      901 atgtcgtcca ccttgatcca gtccccgatt tcgatggagt tgtccagctg caaggccaag
      961 ccgccgagga tgttgcccag cgtgtcctgc atcgcgaacg ccaccacggc ggtgatcatc
     1021 gccgaggtcg cgacgatgct gcccagatcc agtccggcgt agcgcagccg caccagaccc
     1081 caggcgacgt aaccgatgat gacgaagata tcctcggcga tgcgcggcgg ggagacccgc
     1141 gcccatggaa gcacgatgcg gaacaccagc aacccccaca ggcggatcac cgcaatgccg
     1201 gcaccgatga cgccgacctc atgcaacacc gatgcggcgg agagtatccc cagcccctgc
     1261 agcaggccgc tgacgaattg caacacctga caggcgaaga agaacgtcaa cgtgttgagc
     1321 acactcttgc gctcttcctt cagaaaatgg agcaataaaa acccaaggcc cagcgtcatc
     1381 accagcacag gcagcgactc atcgcgccaa aaaaactgca tcgccgtatt ccagaaagac
     1441 atgcctaccc ctcgcatttt gtgtcgggca ttctacacca tcgaatgcgc cgccagcctt
     1501 cggggccgac gccagagcga ccatgccccg gcacgcgtca gacgaatgcg gcgcatgccc
     1561 tcttcgcccg gcacatcgag ctgcaattgc gcgatatcac ccgcacgcaa cgcccgccac
     1621 aagggctgcg catgattggt ttcaaaggat tgcaaggccg tgcgccatgc atgcaagtca
     1681 ccctgctgca aggcgttgcg caaccccgtc cacaccagca actgctcgcc ctcttccgct
     1741 cgccaccgct ctgtccaccc cgagaaggac gcacccgctg ccgccgcgaa catcccggcc
     1801 agttcctcgt ccgaactgac gcaggcataa cggcaaggtg tctggatcgc cgcgacgcca
     1861 ttcccccaca gccatacgct gttgacgggc atctcgccgc gagcctcgcg cagctcgttc
     1921 agcggatgag cgaacaacag catctgcatc tcgttgaaca actgctgcca acgcggccct
     1981 tcctcaccct cgggcaacag tccgcgcaca tcgcgcccgc tcgcctgcga caacggcacg
     2041 gtacgaatat ccggcatcgc gggcagccgc acataccagc gctgcggatg gggagcgacg
     2101 aattccagtc cctgcccggc gaagtgctcg ttcaacgccg cgcagaactg cgccgcctcg
     2161 cccgccgtca cacgaccagc gggttgcatg accagctggg tacgatgcag ctgcatatag
     2221 gccgggtcgg cgcgcaacca atgccccgcc tccagcccgt cgaatgccgc cgacaccgcc
     2281 gcgatcggcg tccccgccga aggcgcgaac aagccgcaca atgcctcttc cagcccgccg
     2341 cctatatctt cagcatcggc cgaaacaccg cgcgccagca tgcgctccag cgcaggcagc
     2401 atcaatcccg cactggcctc ggcggcgata tcggcaggaa ggaacaaatc gggaataacc
     2461 agatggacac ttttcatggc cgcgattcta tcattcgcac accctccttc cggcctaacg
     2521 aacaccaccg ccctgtcgtc gccagccgcc ttgtggcaca ctgacgcccg tttccgctat
     2581 ccatcagtca atcaccttgc gaccttacga actttttatc ggcctgcgct acacccgtgc
     2641 caagcgccgc aatcatttca tctcgtttat ttcactgatc tccatgatgg gcatggggct
     2701 gggggtgatg gcgctgatcg tggtgctgtc ggtgatgaac ggattccaga cggagatacg
     2761 cgaccgcatg ctgggcgctt cgccgcatat ggaagtcgtg gcggatggcg gccgcatgta
     2821 cgactggcct gctgtgctgg agctggtaga aaaacatccg caggtcgctg cggccgcacc
     2881 ctatgtcgcg gggcaaggca tgctgtcgtt cgggcagagc gtgcagggcg tgatggtgcg
     2941 cggcatcgac ccggcgcgcg agacggcgat caccgaactg tccaacaaga tcaaggcggg
     3001 ttcactggat gagctgcgca gcggcgagtt cggcatcgtg ctcggctccg atctggcacg
     3061 cgcgctcggg gtgaagctca acgagaaggt gatgctggtc gcgccgcagg ggcagatcac
     3121 gcccgccggg atgatgccgc gcctcaagca gttccgcgtg gtcggcatct tcgagatcgg
     3181 catggcgccc tatgacaaca gcctcgcgct gatccagatc ggcgacgcac agaagctgtt
     3241 cctgctcggc gatgcggtga ccggcatcag cagcaagctg cacgacatcg aacgcgcccc
     3301 gcaggtcgcc agcgaattgc agcgcgagtt gccgccggag ctgtatacca acgactggac
     3361 gcatcagaac agcaattact tccgcgccgt gcagatggag aagcgcatga tgttcatcat
     3421 cctgtcgctg atcgtcgccg tggccgcgtt caacatcgtc tccacgctgg tgatggcggt
     3481 gaccgacaag caggccgaca tcgcgatcct gcgtacgctg ggcgctgcac catccagcat
     3541 catgaagatc ttcatcgtgc agggcgtggt gatcggggtg atcggcacgc tgtcgggctg
     3601 catcggcggc atcgcgctgg cactgaacct cgacgtggtg gtgccgttca tcgaacacac
     3661 gctcggcgtg caattcctcg ccaaggacgt gtattacatc accgagctgc cgtccgagct
     3721 gcgctaccac gaggtcattc tgatcgcatc gatgtctttc ctgatcagcc tgctggcgac
     3781 gctctacccc agctggcgcg catcgaagac ccaaccggcg gaggcactgc gctatgaata
     3841 atcacaggat cgaggatcaa ggatcgaaga tcgaaccagt catctcctgc cgcgaactga
     3901 gcaagacttt caccatcggc aagcaggacg tgtcggtgct gcgcagcgtc gatctcgatg
     3961 tggcgcgcgg cgaacggctg gccatcgtcg gcgcctccgg ctccggcaag agcacgctgc
     4021 tgcatctgct cggcggattg gacaatgcca gcggcggcag cgtagcgata ctcgggcagg
     4081 atgtgcagaa gatgaacgag acgcgacgcg gcgagttgcg caaccgttcg ctcggtttcg
     4141 tctatcagtt ccatcatctg ctgccggagt tcaccgcgct ggagaacgtg gcgatgccgc
     4201 tgctgatacg cggcatgcgc cgcagggatg ccgagccgct cgccgccgcg atgctggaac
     4261 gcgtcggact ggcacatcgc atcggccatc tgccctccga gctgtccggc ggcgaacgcc
     4321 agcgcaccgc cgtggcgcgt gcgctggtca ccgagcccgc ctgcgtgctg gccgacgagc
     4381 ccaccggcaa tctggaccgc aacagcgccg aggccctgtt cgagctgatg cagcaactca
     4441 acgagcaatt cgacaccagc ttcatcgtcg tcacccacga cctcgacctg gcgggacgca
     4501 tgcagcgcag gctgcggctg gtcgatggag tattgcagga aatatagtca ccggcgaata
     4561 tccccagcca ggctaggaaa agcatctatc aatccgtact gttttcctat ggagatttag
     4621 gaatcggctg atatttttca cccggggaaa gatgcactat gcgtctcaca cgtttctcct
     4681 ccctgagcgt ggtcttaacc aagttaagac attgcccccg gcccccacag atgccggggg
     4741 ctttttttat ctatcgctgc cgccggcgcc agcgccagat caccgccgca cgccagatca
     4801 ctcgcaccag aacatagccc gcaacggcaa gccccagtgc cagcaacggc aatccgaaca
     4861 tcagcggcgc cccaagcgcc tctatccagt cccacagtgc atgggcccag ttatcccaat
     4921 gcatcgccgg gaagggcggc ggctccgtga gcatgccatt ggaagcaccg agtacgcgga
     4981 ggcccagctc ataggccaga aaatacaggg gcacgatggt aaaaggattg gtataggtcg
     5041 tgaccacgat ggccagcggc aaattgacgc gcagccacac cgcgagcagc aacgccgata
     5101 tcatctgaaa cggcccgggt atcagtccgc agaacagccc gaccgccacc cccccggcca
     5161 ccgagcgacg gtgcagatgc cagagattgg gatgatgcag ccacccggag aagggcttga
     5221 acagccgatg gtttctgatc gattcgtgct gcggcagaaa ttggcggaag aatttgcgca
     5281 tgggttcatt ctatcagtca gccggaagcg cccacgccgt gaatgagcgc cacataaaaa
     5341 atcagccagg cgctgaactt gtgccaaagc ccattgtctt ctcacccgac catgccatca
     5401 tggtcgcgaa aaatgcagcc cgaccaaaat caagacgata ataagcaacc gtacaatctc
     5461 taccgatctc ctatgctgtc agccacctta aaacgctacg cctatctgtc catcgcggcc
     5521 gccctcgcca ccatcctgct caagggcata gcctggtgga tgaccggttc cgtcggactg
     5581 ctgtccgatg cgctcgaatc cttcgtcaat ctggcgggcg cgctgatggc gctggcgatg
     5641 atttcgctgg caatggagcc cgccgacgaa aaccatgcct acgggcacgg caaggcggaa
     5701 tatttctcca gcgccttcga aggcttcctc atcctcgtcg ccgccgtcag catcggttac
     5761 acggccatcg aacgcctgct ccatccgcag gccatcgaat cggtcggcat cggtctggcc
     5821 gtgtcggtcg cggcctcgat catcaatctc gtcaccgccc gcgcgctgat ggcggtggga
     5881 cgcaagcaca attccatcac gctggaggcg gacgcacacc atctgctcac cgacgtgtgg
     5941 acttccgtgg gcgtgatcgg cggcgtcgca ctggtgtggc tgaccggctg gctgtggctg
     6001 gatccggtga tcgccttgct ggtggcggcg aacatcgtct ggaccggctg gcaattgctg
     6061 cgccgctccg catcgggact gatggataca tccatcccgg aagaacagat acgcgccatc
     6121 gaaaccgtgc tggaaggcta ccgactgcag gggctggact accacgcgct gcgcaccaga
     6181 caagccggca tgcgcgcctt catcaccctg catgtgctgg tgcccggcag ctggacggtg
     6241 cagcaaggcc acgaccgact ggagcagatc gaggccgaca tccgcaacgt cgtccccaat
     6301 gcgcatttga cgacccatct tgaaccgata gaagatcccg tttcgctatc cgatcagacg
     6361 ctggatcggc atatttgaac aagctgtaga tgatgcaacc gaataagacc attacctgaa
     6421 agagacgtta tgactgcaag aaaaatcggc ctcgccttgc tgttgctcgc cagcgccact
     6481 ccggcactgg cggagaagcc caaggatttc atttccactt acagcacgca ggcaggctcc
     6541 ggcttcactc cctccgccgc aagaggcaag agcttcttcg gcaaggattg gggcgtatcc
     6601 aaagacatgc caaaatgcgc cagctgccac ggcaacgacc tgaaagccaa cggcaagcat
     6661 gtcatcaccg gcaaggtcat cgaaccgttc tcaccgtccg ccaatcccga gcgcttcacc
     6721 agcaccaaga aggtcgagaa atggttcaag cgcaattgca aagaggtggt cggacgcgaa
     6781 tgcacggcag ccgaaaaagc agactttatc caattcgtaa cccaaggaga ttaaccgtga
     6841 aaaaactctt catactcatg ctggctttga ccgcaaccac cgcctatgcc gaaggcggca
     6901 aactgtccgc tgccgaccat ccgaagtgga aagaagaatg cggcagctgc catatcgcct
     6961 a
//
LOCUS       DASUIV010000260         9341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_439826, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000260 DASUIV010000000
VERSION     DASUIV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439826"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFW59_09365"
     CDS             complement(<1..1073)
                     /locus_tag="VFW59_09365"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat-containing protein kinase
                     family protein"
                     /protein_id="HEX5364467.1"
                     /translation="MHTLSLLRAGKLAGIQRLDLSCGLTEFPREIFDLADSLEILNLS
                     GNALSELPDDLPRLHRLRVIFCSDNRFTRVPEVLGQCANLEMVGFKANRIGELPAAAL
                     PEKLRWLILTDNELGELPARIGACTRLQKLMLAGNWLQQLPDELAACRNLELLRIAAN
                     RFAQLPDWLFSLPRLAWLAYSGNPYSDAIEAAATAEHNIANIDWQTLELQHKLGEGAS
                     GVIYRAHHHESDGPVAVKIFKGALTSDGLPRSEKAACIAAGAHPGLIPVVGRISAHPE
                     QTPGLVMSLIAPGFRNLAGPPSLESCTRDIYPADTRFTLPTALNLALGIATVACHLHA
                     RGIMHGDLYAHNIQWNPQGDCLLG"
     gene            complement(1116..2903)
                     /gene="feoB"
                     /locus_tag="VFW59_09370"
     CDS             complement(1116..2903)
                     /gene="feoB"
                     /locus_tag="VFW59_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX5364468.1"
                     /translation="MKRVALLGMPNTGKSTLFNRMTGGAARVGNWPGITVELLSGKIL
                     LGGNMVEIIDLPGIYDLHGYSDDEQVVRHFLHDNVPDLALVILNATQIERQMSLLLQL
                     KQLNMNVVVLLNMADEAKKFGITIDSKKMAEMLDMPIFMLSGKYGTGYQEAMQAVTKA
                     LRYPTPGMAEQLRSQLEQDEHIESEMARVMKHAVQIPARLPDNLTEKLDRVMLHPWLG
                     LPIFFGIMYLLFQGIFLLGQPLQTGIAELLGWLRTTALEPALSGLPVLLNGLLLDGIY
                     NGVATVAAFVPIIVLFFLFMAMVEDSGYLSRAAFLMDALMARLGLDGRGFVMLLMGFG
                     CNVPALMGTRVMRSRSLRLLTMLVIPFSLCSARLQVFVFITAALFSPQYAAPVLFSLY
                     LFSFAAAILTALLFKGSFKNSEPFVLELPPYRFPTARQMWLRGWLEVQHFLRRATKFI
                     IAGVVLVWLLTHLPLSAVPASSDTLAGMIGGWLHPVFAPIGVDENLTIALLFGFVAKE
                     IVIGSLAVIYGMSGDALGVALSQQLDWVQAYSFMLFTLSYTPCMSSIATLRSESKQAG
                     YTALVIGWSLALAWLVSFVFYQGARALGY"
     gene            complement(2900..3145)
                     /locus_tag="VFW59_09375"
     CDS             complement(2900..3145)
                     /locus_tag="VFW59_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX5364469.1"
                     /translation="MSALTLASLLPGDTATIVAIHAEEALHQRLQALGFRSGKKVELI
                     RAAAFSGPLQVRVGTTDVLLRRSEAAKITICKGIAEI"
     gene            3260..4144
                     /gene="dapA"
                     /locus_tag="VFW59_09380"
     CDS             3260..4144
                     /gene="dapA"
                     /locus_tag="VFW59_09380"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5364470.1"
                     /translation="MIKGSIVAIVTPMHEDGSLDLAAFRALIDFHVDQGTDGIVVVGT
                     TGESPTVDVEEHELLIAEAVKHVAGRIPVIAGTGANSTREAIELASFSKKAGADASLT
                     VVPYYNKPTQEGLYQHFKAIAEAVDMPHILYNVPGRTVADMSNDTVLRLAQIPNIVGI
                     KDATGNIERGSDLLARAPKDFAIYSGDDASTLALMLLGAHGTISVTANVAPKLMHEMC
                     MAALNDEVAKARAINFQLLGLHRHLFVEANPIPVKWAVARMGKMKNTLRLPLTPLSSS
                     AEAVVEAAMRQAGIIAHH"
     gene            4188..5303
                     /gene="bamC"
                     /locus_tag="VFW59_09385"
     CDS             4188..5303
                     /gene="bamC"
                     /locus_tag="VFW59_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX5364471.1"
                     /translation="MKALHIGISTVVLVSLAGCGSMGLGNKRIDYRSGAAQVPALEIP
                     PDLTAPAGDDNFKVPGAANVATYSDYSKGGVAVQAGRSAVLPEVKGVQLQRNGAQRWL
                     VVGDKAENVWPVVRAFFQENGLTIASEDQAAGVMETDWAENRAKIPQDAVRSVLGKVF
                     DGLYSSGQRDQYRARLERAKDGASTEVYLTHRGMEEVLSSDGTSSKWQTRPADPEMEA
                     VMLQKLMVRFGGSEVQAASAVNSSGTAAPSGAVVGGDGKASLLEVFDGSKVLTVNDAF
                     DKAWRRVGLALDQAGLAVEDKDRAKGIYFLRSTQAERSWVDDLMFWKDDTSSDRRYRV
                     NVKDGGASCEVRVVDQDDANDDVAAGILEKIYKHIGQ"
     gene            5308..6075
                     /locus_tag="VFW59_09390"
     CDS             5308..6075
                     /locus_tag="VFW59_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5364472.1"
                     /translation="MRFASLGSGSEGNALLVEVAGTRLLMDCGFGLRESISRLAGLGV
                     SPEQLSGIVVTHEHGDHSAGVARLARKFSLPVWLTHGTRRKLSKHLAGVERIHEIDPH
                     QPFAIGDIEITPYPVPHDAAEPVQFVFADGARRLGVLTDAGSSTPHIESMLNACHALV
                     LECNHDSDMLMNGDYPYSLKQRVGGRYGHLNNLESASILARLDVSKLQHLVAAHLSRS
                     NNTTALAVDALSAALNCEAGWIGVATQGEGFSWREIA"
     gene            complement(6145..6351)
                     /locus_tag="VFW59_09395"
     CDS             complement(6145..6351)
                     /locus_tag="VFW59_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364473.1"
                     /translation="MNRSALVAALLALALSACGEKPAEAPAVEAAPAVVAAPAPAVEA
                     ASAPVAAEAASAPAAAEVAAPAAK"
     gene            complement(6505..6996)
                     /locus_tag="VFW59_09400"
     CDS             complement(6505..6996)
                     /locus_tag="VFW59_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364474.1"
                     /translation="MSDELLQHTKLDGIADYTAALDTLCQLAQRNLYLFENDFEGLGF
                     NSEARYETLRHFLLSNPAHRLFVLAHSTHYLSTRCPRMMMLLSQFGTSMSIRQTPKSL
                     QHLTEPFSVADEEHYVRRFHFDDPRGILAKHDPSNALVLKARFMEMWGATHSSISAAK
                     LGL"
     gene            complement(7009..8136)
                     /locus_tag="VFW59_09405"
     CDS             complement(7009..8136)
                     /locus_tag="VFW59_09405"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5364475.1"
                     /translation="MKTIQTLLGGLSVDEFLRDYWQKKPLLIRKALPAFEGLLDPGHL
                     VDLACQEDVQARIVTQQSGKFDLQHSPFEPEDFAHFGKKKWTVLVQGVNHHLPEAAEL
                     LKHFAFIPHARLDDLMVSYAPKGGGVGPHFDSYDVFLLQGKGHRRWQISTQADRSLIE
                     GAPLRILRDFKVEQEWVLETGDMLYLPPHCAHNGIAEDDCMTYSIGFRTPAYQELAEQ
                     FLVYLQDRIQVEGMYADPDLKTQKHPSEIGAAMLRQVGDAIKQVRWDKEDIANFLGCY
                     LSEPKPHIYFEGPQRPLNPAKFRKTIQTHGIELDLKSQMLCHGGWIFMNGEAFDVAEG
                     DYTLLRELADGRGLPAVPDATDECIELLYQWYLDGYLFPPQ"
     gene            8238..8717
                     /locus_tag="VFW59_09410"
     CDS             8238..8717
                     /locus_tag="VFW59_09410"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5364476.1"
                     /translation="MHVDRLTAGMEAPHFELPDADMENFVLTSLREQKNVVLYFYPRD
                     DTPGCTLEANEFSDFADDFARLDTIVIGISRDDCLSHASFRDKYGLSVLLLSDPDARV
                     CKRYGVMVEKEGDAHRKHVIQRATFVIDKHGKLRHAMYDVHAHGHAEEVLNLIKEMK"
     gene            8714..9202
                     /locus_tag="VFW59_09415"
     CDS             8714..9202
                     /locus_tag="VFW59_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5364477.1"
                     /translation="MKIAKDTVVSLLYELFDADGELLEKLDEPVSYLHGGYDGIFPLV
                     EEALHGKAVGDTCSVTMKPDDAFGEYDHQLVEVEPRSSFPKDIELGMQFEGGPDDSEE
                     DDFLLYTVVDVTDDEVTVDGNHPLAGKTLTFNCTVTGVRAATQEELEHGHVHDGDNEH
                     LH"
BASE COUNT         1819 a         2614 c         3079 g         1829 t
ORIGIN      
        1 cccagcaggc agtcgccctg cggattccac tggatgttgt gcgcgtacag gtcgccgtgc
       61 atgatgcccc gcgcatgcag atggcaggcc acggtggcga tgccgagggc gaggttgagc
      121 gcggtcggca gcgtgaagcg ggtgtcggcc gggtagatgt cgcgggtgca ggactccagg
      181 ctgggcgggc ccgcgagatt gcgaaagccg ggggcgatca gcgacatcac cagtcccggc
      241 gtttgttcgg ggtgtgcgct gatcctgccg acgacgggta tcagcccggg gtgcgcaccg
      301 gcggcgatgc aggcggcttt ctcgctgcgc ggcaggccgt cgctggtgag cgcgcccttg
      361 aatatcttga cggccaccgg cccgtcgctt tcgtggtggt gggcgcggta gatcacgccg
      421 gatgcgccct cgccgagctt gtgttgcagc tccagcgtct gccagtcgat attcgcgatg
      481 ttgtgctctg ccgtggcggc ggcctcgatg gcgtcgctgt acggattgcc cgagtaggcc
      541 agccatgcca gacggggcag cgaaaacagc cagtcgggca gctgcgcgaa gcggttcgcg
      601 gcgatgcgca acagctccag gttcctgcag gcggccagtt cgtcgggcag ctgctgcagc
      661 cagttgcccg ccagcatgag tttttgcagc cgggtgcatg cgccgattcg cgcgggcagt
      721 tcgccgagct cgttgtcggt caggatcagc cagcgcagtt tttccggcag ggctgctgcg
      781 ggcagctcgc cgatccggtt ggccttgaag cccaccatct ccagattagc gcactgaccc
      841 agcacttccg gcacgcgggt gaaacggttg tccgagcaga agatcacgcg cagcctgtgc
      901 agcctcggca ggtcgtcggg cagttcggac agggcgttgc cggagagatt gagtatctcc
      961 agcgagtcgg ccagatcgaa tatttcgcgg ggaaattccg tcagtccgca ggacagatcg
     1021 aggcgctgga tgccggccag ttttccggcg cgcagtaacg aaagagtgtg catggcgtga
     1081 tcagggcagg gcggtgagtg gacagtcgcc gcaggtcagt atcccaatgc cctcgcgccc
     1141 tgatagaaga cgaagctgac cagccaggcc agtgccagcg accagcctat gaccagcgcg
     1201 gtgtaacccg cctgtttgga ttcgctgcgc agcgtggcga tggaggacat gcagggcgtg
     1261 tagctcagcg tgaacagcat gaagctgtag gcctgcaccc agtccagttg ctggctcagc
     1321 gcgacgccca gcgcgtcgcc gctcatgccg tagatcaccg ccagcgaacc gatcacgatt
     1381 tccttggcga cgaaaccgaa cagcagcgcg atggtgagat tctcgtcgac cccgatcggg
     1441 gcgaacaccg gatgcagcca gccgccgatc atgcctgcca gcgtgtcgct gctggcgggg
     1501 acagcggaga gcggcagatg ggtgagcagc cacaccagca ccacgcccgc gatgatgaac
     1561 ttggtggcgc gacgcaggaa gtgctgtacc tccagccagc cgcgcagcca catctgccgc
     1621 gcggtgggga agcggtaggg cggcagctcc agcacgaagg gttcgctgtt cttgaacgat
     1681 cccttgaaca gcagcgcggt caggatcgcg gcggcgaagc tgaacagata caggctgaac
     1741 agcaccggtg cggcgtattg cggcgagaac agcgcggcag tgatgaacac gaacacctgc
     1801 agtctggccg agcacagcga gaacgggatc accagcatgg tcagcaatcg caggctgcgc
     1861 gagcgcatca cgcgggtgcc catcagcgcg ggcacgttgc agccgaaacc catcaacagc
     1921 atcacgaagc cgcgcccgtc cagccccagt ctggccatca gcgcatccat caggaaggcg
     1981 gcgcgcgaca gatagccgct gtcctcgacc atcgccatga acaagaagaa cagtacgatg
     2041 atgggcacga aggcggcaac ggtcgccacg ccgttgtaga tgccgtccag cagcaggccg
     2101 ttcagcagta cgggcaggcc ggacagcgcg ggttcgagcg ccgtggtgcg cagccagccc
     2161 agcagttccg cgatgccggt ttgcagcggc tggccgagca ggaagatgcc ctgaaacagc
     2221 agatacatga tgccgaagaa gatcggcagg cccagccagg ggtgcagcat cacccggtcg
     2281 agtttttcgg tgaggttgtc cggcaggcgc gccggtattt gcaccgcatg tttcatcacc
     2341 cgcgccatct cggactcgat gtgctcgtcc tgttccagct ggctgcgcag ctgttccgcc
     2401 atgccgggcg tggggtagcg caatgccttg gtgacggcct gcatggcttc ctgatagccg
     2461 gtgccgtact tgccgctgag catgaagatc ggcatgtcga gcatctctgc catctttttg
     2521 ctgtcgatgg tgatgccgaa cttcttcgcc tcgtcggcca tgttcagcag caccaccacg
     2581 ttcatgttga gctgcttgag ctgcagcagc aggctcatct ggcgctcgat ctgggtggcg
     2641 ttgaggatca ccagcgccag atcgggcacg ttgtcgtgca ggaaatgccg caccacctgc
     2701 tcgtcgtcgg agtagccgtg cagatcgtag atgccgggca gatcgatgat ctcgaccatg
     2761 ttgccgccga gcaggatctt gccggagagc agctcgacgg tgatgccggg ccagttgccg
     2821 acgcgcgccg caccgccggt catgcggttg aacagcgtgg acttgccggt gttgggcatg
     2881 cctaatagag cgacgcgctt catatttcgg ctatcccttt gcatatcgtg atcttggccg
     2941 cctcgctacg gcgcagcagc acgtcggtgg tgccgacgcg cacctgcagc ggcccggaga
     3001 aggccgccgc ccggatcagt tcgacctttt tgccgctgcg gaatcccaga gcctgcaggc
     3061 gctgatgcag cgcttcttcg gcgtggatgg cgacgatggt cgcggtgtcg ccggggagca
     3121 ggctggctag ggtcaaggcg gacatgatgg cgttcagatt gagtccgcgc attattccac
     3181 agcgtcggcg ctcttgtcgc gtcaggtgtt ccgattgagg tagaatgccg tctttgattt
     3241 tttgctggca gataaagaaa tgatcaaggg cagtatcgta gcaatcgtca cccctatgca
     3301 tgaggacggc agtctcgatc tggcggcgtt tcgtgcgctg atcgatttcc atgtcgatca
     3361 ggggacggac ggtatcgtgg tggtcggcac gaccggcgag tcaccgacgg tggatgtgga
     3421 agagcacgag ttgctgatcg ccgaggcggt gaagcatgtc gccgggcgca ttccggtgat
     3481 cgccgggacg ggggcgaatt ccaccaggga ggccatcgag ctggcgtctt tctcgaagaa
     3541 ggcgggtgct gatgcatcgc tgacggtggt gccttactac aacaagccga cacaggaagg
     3601 cctgtaccag cacttcaagg ccatcgccga agccgtggat atgccgcaca ttctgtacaa
     3661 cgtgccgggc cgtaccgtgg ccgacatgag caacgacacc gtgctgcgac tggcgcagat
     3721 tcccaatatc gtcggcatca aggacgcgac cggcaatatc gagcgcggca gcgatctgct
     3781 ggcgcgcgca ccgaaagact tcgcgatcta cagcggtgac gatgccagca cgctggcgct
     3841 gatgctgctc ggtgcgcacg gcacgatttc ggtgaccgcc aacgtcgcgc cgaagctgat
     3901 gcacgagatg tgcatggccg cgctgaacga cgaagtcgcc aaggcgcgcg cgatcaattt
     3961 ccagttgctc ggtctgcacc gtcatctgtt cgtcgaggcc aacccgatcc cggtgaaatg
     4021 ggcggtggcg cgtatgggca agatgaagaa tacgttgcgt ctgccgctga ctccgttgtc
     4081 gtcttccgcc gaagcggtgg tcgaggcggc gatgcgtcag gcaggtatca ttgcccatca
     4141 ttaatgttgt ctgccattaa ccaataatgt tgtctgagag agaaaccatg aaggctttgc
     4201 atatcgggat ttccactgtc gtgctggtgt cgctggcagg gtgcggttcg atggggttgg
     4261 gcaacaaacg catcgattac cgttccggtg cggcgcaagt gcccgcgttg gagattccgc
     4321 ccgatctgac tgcgcctgcc ggcgatgaca acttcaaggt gcccggggcc gccaacgtgg
     4381 cgacctattc cgactacagc aaaggcggcg tagccgtgca agcgggccgc agcgcggtgt
     4441 tgccggaagt gaagggcgtt cagctgcagc gcaacggcgc acagcgctgg ctggtcgtgg
     4501 gcgacaaggc ggagaatgtc tggccggtgg tcagggcttt ctttcaggag aacggcctga
     4561 ctatcgccag cgaagatcag gcagccggcg tgatggaaac cgattgggcg gaaaaccgcg
     4621 ccaagatacc tcaggacgcc gttcgcagcg ttctgggcaa ggtgttcgat ggtctctatt
     4681 cttccgggca gcgcgatcag tatcgcgccc gtctggagcg cgccaaggac ggtgccagca
     4741 ccgaggtcta tctcacccat cgcggcatgg aagaggtatt gtccagcgat ggcaccagct
     4801 ccaagtggca gactcgtcca gccgacccag agatggaagc ggtcatgttg cagaagctga
     4861 tggtgcgttt cggcggcagc gaagtccagg cggcgagtgc ggtgaattca tccggtacgg
     4921 cggctccctc cggtgcggtc gtgggcgggg atggcaaggc cagcctgctg gaggtgttcg
     4981 atggcagcaa ggtgctgacg gtgaacgacg cgttcgacaa ggcttggcgc agggtcggtc
     5041 tggcgctcga tcaggcggga ctggccgtgg aagacaagga tcgcgccaag ggaatctact
     5101 tcctgcgctc gactcaggcc gagcgtagct gggtggatga tctgatgttc tggaaggatg
     5161 atacgagctc cgaccggcgc tatcgcgtga acgtcaagga tggcggcgca agctgtgaag
     5221 tgagggttgt cgatcaggat gatgccaacg acgacgtggc tgcaggcatc ctcgagaaga
     5281 tctacaagca catcggccag taaccgcatg cggtttgcct ctctgggcag cggcagcgag
     5341 gggaacgcgc tgctggtgga ggtcgccggg acgcggctgt tgatggattg cggtttcggc
     5401 ttgcgcgaga gcatctcgcg tttggccgga ttgggcgtct ctcccgagca gctgagcggt
     5461 atcgtcgtca cccatgagca tggcgatcac agcgccggcg tggcccggct ggcgcgcaag
     5521 ttcagcctgc cggtgtggct gactcatggc acacggcgca aactgtcgaa gcacttggct
     5581 ggggtcgagc ggatacacga gatcgacccg caccagccct tcgccatcgg cgatatcgaa
     5641 atcaccccct atcccgtccc tcatgatgcc gccgaaccgg tgcagttcgt gttcgccgat
     5701 ggcgcgcgcc gtctgggtgt gctgaccgat gccggaagca gcacgccgca catcgaatcc
     5761 atgctgaatg cttgccatgc gctggtgctg gagtgcaacc atgacagcga catgctgatg
     5821 aacggcgatt atccctatag tctcaagcag cgcgtgggcg ggcgctacgg tcatctgaac
     5881 aatctggagt cggcgtctat cctcgccaga ctggatgtga gcaagctcca gcatctggtc
     5941 gccgcacatc tgagccgcag caataacacc acagcacttg cggtggacgc actaagcgcg
     6001 gcgctgaatt gtgaggccgg gtggataggc gtggcgacgc agggggaggg attttcttgg
     6061 cgcgagatcg cctgaggatg gagcgctgaa tgcaaaaaag ccgacttgcg tcggcttttt
     6121 tattgcccga aggcgtgttg cttattactt ggcagcagga gcagcaactt cagcagcagc
     6181 tggagcggat gcagcttcag cagcaactgg agcggatgca gcttcaacag caggagcagg
     6241 agcagcaact acagcaggag cagcttcaac agctggagct tcagctggct tttcgccaca
     6301 agcggacaga gccagagcca acagagcagc aacgagagcg gaacgattca ttttaaatac
     6361 ctcaggagtt gttaaatagt taagagttaa attttttttc ttgattctcg aagggctcga
     6421 aatccaaggc ggcgcatttt agcatttgta tatataaaat ccagtagttt ggcaactatt
     6481 tttccccggt gccggggcat ggggctaaag ccccagcttg gcggcgctga tgctggaatg
     6541 ggtggcgccc cacatctcca tgaaacgcgc cttcagtacc agcgcgttgg acgggtcgtg
     6601 tttcgccaga atgccgcgcg ggtcgtcaaa gtggaagcgg cggacatagt gctcttcgtc
     6661 ggccacggaa aagggttcgg tgaggtgctg caggctcttc ggggtctggc ggatggacat
     6721 gctggtgccg aactggctga gcaacatcat catccgtggg caacgggtgg acagataatg
     6781 ggtgctatgc gcgagcacga acagccgatg ggcgggattg ctcagcagga agtggcgcaa
     6841 cgtctcgtaa cgcgcctcgc tgttgaagcc cagaccttcg aagtcgttct cgaacaggta
     6901 caggttgcgc tgtgccagct ggcacagggt gtcgagtgcc gcagtgtagt cggcgatccc
     6961 gtccagtttg gtgtgttgca gcaactcgtc gctcatggct ggctcctgct attggggagg
     7021 aaaaagatac ccatccagat accactgata aagcagctcg atgcactcgt cggtggcgtc
     7081 cggtacggcc ggtaagccgc gcccatcggc cagttcgcgc aacagggtgt agtcaccctc
     7141 ggcgacatcg aatgcttcgc cattcatgaa tatccagccg ccatggcaca gcatctggct
     7201 cttcaagtcc agctcgatgc catgtgtctg tatggttttc cggaatttcg ccgggttgag
     7261 cgggcgttgc ggcccctcga agtaaatatg gggcttgggt tcggagaggt agcagccgag
     7321 gaagttcgcg atgtcttcct tgtcccagcg tacctgtttg atggcgtcac cgacctgacg
     7381 cagcatggcc gcaccgatct ccgatgggtg tttctgcgtc ttcaggtcgg gatcggcgta
     7441 catgccctct acctggatgc ggtcttgcag gtacaccagg aattgttcgg ccagttcctg
     7501 ataggccggg gtgcggaagc cgatggaata ggtcatgcag tcgtcttcgg cgatgccgtt
     7561 gtgcgcgcag tgcggcggca gatacagcat gtcgccggtt tccagtaccc attcctgctc
     7621 taccttgaag tcgcgcagga tgcgcagcgg cgcgccttcg atcaggctgc ggtccgcctg
     7681 ggtggaaatc tgccagcggc ggtgcccctt tccttgcagc aggaatacgt cataggagtc
     7741 gaaatgcggc ccgacgccgc cgcccttggg cgcatagctc accatcaagt cgtccagcct
     7801 ggcatggggg atgaatgcga agtgtttgag cagttcggca gcctcaggca ggtgatggtt
     7861 gacgccctgc accagtaccg tccatttttt cttgccgaaa tgcgcgaagt cttcgggctc
     7921 gaacggcgag tgctgcaggt cgaatttgcc gctctgttgg gtgacgatgc gcgcctgcac
     7981 atcctcctga caggcgagat ccactaagtg gcccggatcg agcaagcctt cgaaggcggg
     8041 tagcgccttg cggataagca gcggtttctt ttgccagtag tcgcgcagga attcgtcgac
     8101 gctcaggccg cccagcagtg tctgtatggt tttcatgccg acgattatag accggagagc
     8161 ctgcgtccgg gcagggcgta aaaactttta tggcccgccg gaaaagtgtt taaaatggtc
     8221 agcaggggga tagacctatg cacgttgatc ggcttaccgc aggaatggag gcgccgcatt
     8281 ttgaactgcc ggatgccgac atggagaact tcgtgctcac ctcgctgcgg gagcagaaga
     8341 atgtcgtgct gtatttctat cccagggacg acacgccggg ctgcacgttg gaagccaacg
     8401 agttcagcga tttcgcagat gactttgcca gactcgatac catcgtgatc ggaatcagtc
     8461 gtgacgactg tctgagtcac gcgtcgttcc gcgacaagta tggtttgtcg gtgttgctgt
     8521 tgtccgatcc ggatgcgcgc gtctgcaaac gctacggcgt gatggtggaa aaagaggggg
     8581 atgcgcacag aaagcatgtg atccagcgcg ccaccttcgt gatcgacaag catggcaagc
     8641 tccgccatgc gatgtacgac gttcatgcgc acggacatgc agaagaagtt ctcaatttga
     8701 taaaggaaat gaaatgaaaa tcgccaaaga caccgttgta tcgctgcttt atgaattgtt
     8761 cgacgccgat ggcgagctgc tggaaaagct ggatgagccg gtcagctatt tgcatggcgg
     8821 ttatgacggt atctttccac tggtcgaaga ggccttgcac ggcaaggctg tcggggatac
     8881 ctgcagcgtg accatgaagc cggatgacgc ctttggagaa tacgatcacc aactggtgga
     8941 ggttgagccg cgcagctcct tccccaagga catcgaactc ggcatgcagt tcgaaggcgg
     9001 gccggacgat tcggaagagg acgacttcct gctctacacc gtggttgacg tgactgacga
     9061 tgaagtgacc gtggatggca atcatccgct ggcgggcaag acattgacct tcaattgcac
     9121 cgtgaccggt gtgcgtgccg cgacccagga agagctggag cacggccatg tacacgatgg
     9181 cgacaacgaa cacctgcatt agataacctc cctcttcggg agggggcgct ccgggttctt
     9241 cctgtgctcg tgtcgcagca gtctcaggga ggtattttct gtggtgtatt tccggtattt
     9301 ggttgccgaa tgaaaaactg atatacgcag gacaagctag c
//
LOCUS       DASUIV010000261         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_347409, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000261 DASUIV010000000
VERSION     DASUIV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347409"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1655)
                     /locus_tag="VFW59_09420"
     CDS             complement(387..1655)
                     /locus_tag="VFW59_09420"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX5364478.1"
                     /translation="MTILLISFALLLVIGLPIAIAMMGASLLVILVEGIPISVVAQRV
                     VTGVQSFPLLAIPLFTLAGSLMNDSGISERLFSFTRAFVGHIRGGMAQTAIVGNIFLS
                     GISGSSVADCAATSRVFVPQLAKAGYGAGFAAALCAACATLGPIIPPSILMVIYAWQA
                     NISLGDLFWAGIFPGLLMALAMVLLTAYIARRRRFPKDQAFAWGRLWIEFRGAFWALL
                     MPVLILVGFRMGVFTATEIAGVAAAYALIVGLFIYRTLRWSHLPKVLLSTAKETAVIL
                     LIVAAAAPFSWILGIEQAPQLISEALKGATDSPWVILMLLNMVLLILGCFMETIAIMI
                     ILVPILIPVLNQYQIDLTHFGIILLVNLTIGQLTPPVGVLLFVASSVSKVRLGLLVRE
                     VVPYVAVLILTLLVITNVPALSLWLPQALK"
     gene            complement(1652..2188)
                     /locus_tag="VFW59_09425"
     CDS             complement(1652..2188)
                     /locus_tag="VFW59_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5364479.1"
                     /translation="MAKLCKMLGRVDDVIARAEDVFIIFAHGAVAGLVLLSVVLRYVF
                     NDPLTWGEELIVGLFTWMVFVAAAAAVRSQMHIRIDVIAPVFRNPKMNWLNTLTLVIG
                     IVIIVTMVWACYEQVLQELVVESPMLGVSKAWFVASMPVGLILMLIHVLRSWLDHGAA
                     SVFRGETEILIASEGGAK"
     gene            complement(2222..3376)
                     /locus_tag="VFW59_09430"
     CDS             complement(2222..3376)
                     /locus_tag="VFW59_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5364480.1"
                     /translation="MIVRSYVRPERRPIRYRAFITGHPRVAPSIWLGNLMSHQGVFMY
                     LFAARKLLASTVAAALFAALTMVSTTASAATTLRFGHANNTGEIATDLFQEFADKVTQ
                     RTKGEVTIKVFPAEQLGKEGDLIQQVKSGALDISAPSMPSLSGLVPGLEIASAPFLWT
                     DWAQAEKVITGPAFQPQFDELRDKHNVLMLSKIWYWGWRNLTTSTRAVHKPEDLVGLK
                     IRVPESPVWVEMIRAMGGAPTPIPFGEVYTALQQKTVDGQENPIPTIFSRKFYEVQAH
                     MSMTRHMLQNNTMIINKRSFDKLSPDMQKIVLEEAAVASTKNSKLQQERETSMLETIR
                     KSGKTAIVDQPDRAAFAEKMAPAFQKLEARWGAENLQRVKAAVAASAGKK"
     gene            complement(3373..4566)
                     /locus_tag="VFW59_09435"
     CDS             complement(3373..4566)
                     /locus_tag="VFW59_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5364481.1"
                     /translation="MMSTIVRVDVHEFEFDATNLARDGASQSVTYKKGATTRLGKYAV
                     SIHTDDGGRGDYVALWVSSPAALGQTLMLAPRLIGKDARQRIAIYEDLKRDIRQFDHM
                     GHGAIDIALWDWAGRHYNASVSELLGGYRKRLPAYASTYHGDRAGGLSSKEAFADFAE
                     ACHGIGYRAFKIHGWNEGNAREEAENVLHVAKAVGDRMTLMLDPACELRTFADALYVG
                     RACDDARFFWYEDPFRDGGVSAFAHQRLRSMIRTPLLQTEHVRGVEPKADFVLAGGTD
                     FLRVDPEYDMGITGALKVAHLAEALGLDCEIHASGPAHRHLMSAMRNSNFYEVALVGP
                     DCPNAMPPVYRCGYTDQLECVDAAGTVPVPTGPGLGVDYDWEWINANRTALHVFDRSG
                     SRVTR"
     gene            complement(4589..4723)
                     /locus_tag="VFW59_09440"
     CDS             complement(4589..4723)
                     /locus_tag="VFW59_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364482.1"
                     /translation="MNCHFDISLRTALWFDARDKTRLKNGNFNFNNIYRHIAVEEMFK
                     "
BASE COUNT         1095 a         1493 c         1478 g          944 t
ORIGIN      
        1 gtgaaaaacg gcatgatcag tctgaacgac aaacccggat tggggctgga gctgctgccc
       61 gatctgcaca agcgcgctga tgctaccgtg agaatttcaa agggctgatg aaggccatgc
      121 gccaagcgcg agcgggccag agccatgaat gcctcagcaa gccatgacag ggcgctccgc
      181 ttgcatcgcc atgcagcgcg gactggtggt taatgcgcga tagtgccaag ttcagcatac
      241 ggccccctaa ccgatagcac aagccgctgg ctatcggctg gctatcggtt acccagaatg
      301 caccgagtac gatcaggcca tccatcagct gcaggggtgg tggtattgat ggcgatatct
      361 tccatggagc atcagtgcca gcttgatcat ttcagcgctt gtggcaacca cagcgacagc
      421 gccgggacat tggtgatcac cagcagagtc aagatcagca cagccacata cgggaccacc
      481 tcgcggacca gcaagccgag ccttaccttg gacaccgaac tggcaacgaa cagcagcacc
      541 ccaactggag gcgtcaactg gccgatggtc aggttgacca gcaggataat gccgaagtgg
      601 gtcaggtcga tctggtactg gttcagcacc ggaatcagga tcggcacgag aatgatcatg
      661 atcgcgatgg tttccatgaa gcagccgaga atcaacagca ccatgtttaa cagcatcagg
      721 atgacccagg gactatcggt ggcgcccttc agcgcctccg agatcaattg gggggcctgt
      781 tcaatgccga gtatccagct gaaaggggcg gcggcggcga cgatcagcag gatgacggcg
      841 gtttccttcg ccgtggacag caggaccttt ggcaaatgcg accagcgcaa ggttcggtag
      901 atgaacagcc cgacgatcaa tgcataagcg gccgcgacac ctgcaatctc ggtggcggtg
      961 aaaacgccca tccggaagcc gaccagaatc agtactggca tcagcaacgc ccagaacgcg
     1021 ccgcggaact cgatccacag acgtccccag gcgaacgctt gatccttcgg gaaacgccgt
     1081 ctgcgggcga tatacgccgt cagcaacacc atggccagcg ccatcagcag gcccgggaag
     1141 ataccggccc agaacaggtc gccgagcgag atgttggctt gccacgcgta gatcaccatc
     1201 aggattgacg gcgggatgat cgggccgagc gtggcacagg cggcgcacag cgcggccgcg
     1261 aagccggcgc catagccggc cttcgccagc tgcgggacga acacgcgcga cgtggccgcg
     1321 cagtcggcga ccgatgagcc agaaatgcca gacaggaaga tgttgccgac gatcgccgtt
     1381 tgcgccatgc cgccgcggat gtggccaacg aacgcgcgcg tgaacgagaa aagtcgttcg
     1441 ctgatgcccg aatcattcat cagcgaaccg gccagcgtga acaatggtat ggccagcaac
     1501 gggaacgact gcacgccggt gacaacccgc tgcgcgacga ccgatattgg gatgccttcc
     1561 accaggatga ccagcagcga tgcgcccatc atcgcgatcg cgatcggcag gccgatgacc
     1621 aacagcaaag caaatgaaat cagcaggatt gtcatttggc tccaccttca ctggcgatca
     1681 gaatttcagt ttcaccgcga aacaccgaag ccgctccgtg atcgagccag gacctgagta
     1741 catgaatcaa catcaggatc agaccgaccg gcatcgacgc gacgaaccag gccttcgaga
     1801 cgccgagcat cggcgactcg acgaccagtt cctgtaacac ctgctcatag caggcccaga
     1861 ccatcgtcac gatgatcacg atgccgatga ccaaagtcag cgtgttgagc cagttcatct
     1921 ttggattgcg aaagactggc gctatcacat cgatccggat gtgcatttgc gatctgaccg
     1981 cagcggcggc ggcaacgaag accatccagg tgaagaggcc gacgatcaat tcctcgcccc
     2041 aagtcagcgg gtcattgaac acgtagcgca gcacgacaga cagcaataca agtccggcga
     2101 ctgcaccgtg ggcgaagatg atgaaaacat cctctgccct ggcgatgacg tcatcgaccc
     2161 ggcccagcat cttgcaaagt ttggccacag gtgtctcccg agtcagtatt gcaggtgccg
     2221 cttacttctt gccagccgag gcagcaacgg cggctttaac ccgctgtagg ttttctgcgc
     2281 cccatcgcgc ttccagtttc tgaaaggcag gcgccatttt ttccgcaaag gcggcgcgat
     2341 ccggttgatc gacgatggct gtcttgccgc tcttgcggat ggtctccagc atgctggtct
     2401 cgcgctcctg ctgcagcttc gagtttttcg ttgatgccac cgccgcttct tccaacacga
     2461 ttttctgcat gtccggcgac agcttgtcga atgaacgctt gttgatgatc atcgtgttgt
     2521 tctgcaacat gtggcgcgtc atcgacatgt gcgcctgcac ttcatagaat ttgcgcgaga
     2581 agatcgtcgg aatcgggttt tcctgtccat ccacggtttt ctgctgcagc gctgtgtaga
     2641 cctcgccaaa cgggatcggt gttggtgcgc cacccatcgc tcggatcatc tcaacccaga
     2701 ccggcgattc cggtacgcgg atcttcaggc cgaccagatc ttccggtttg tggacggcgc
     2761 gggtactcgt ggtcaggttg cgccatcccc agtaccagat cttcgacagc atcagcacat
     2821 tgtgcttgtc gcgtaattca tcgaactgcg gctggaacgc cgggccggtg atgacctttt
     2881 cggcttgggc ccagtcagtc cacaggaacg gtgcgctggc gatctccaga ccgggcacca
     2941 ggcccgacaa cgacggcatc gacggcgcgg aaatgtccaa tgcgccggac ttgacctgct
     3001 ggatcagatc gccttccttg ccaagctgct cggccgggaa gaccttgatc gtgacttcgc
     3061 ccttggtgcg ttgggtcacc ttgtcggcga actcctgaaa caggtcggtg gcgatctcgc
     3121 cggtgttgtt ggcgtggccg aagcgcagcg tggtggctgc cgaggcagtt gtggacacca
     3181 tcgtcagcgc ggcgaatagc gccgcagcaa cggtagaagc gagcaatttc cgggcagcga
     3241 agagatacat aaatactcct tggtgagaca tgaggttgcc cagccaaatg gatggcgcaa
     3301 cacgagggtg cccggtgatg aacgcccgat accggatcgg gcggcgttca gggcggacat
     3361 aggatcgaac gatcatcggg tgacccgact tcccgaacga tcgaaaacat gcagcgcagt
     3421 gcgattggcg ttgatccatt cccaatcgta gtcaacgcca agtcctggcc cggtgggtac
     3481 cggcaccgtg ccggcggcat ccacgcattc cagctggtcc gtgtaaccac agcgataaac
     3541 cggcggcatg gcgttcgggc aatccgggcc caccagcgcg acctcgtaga aattggaatt
     3601 acgcatggcg gacattaaat ggcgatgggc cgggccactg gcgtgaatct cacagtccag
     3661 gccgagtgct tcggccaggt gcgcaacctt cagtgcgccg gtgatgccca tgtcgtactc
     3721 cggatccacg cgcagaaaat ccgtgccacc agccaaaacg aagtcggcct taggctccac
     3781 gccgcgcaca tgctccgtct gtagcagcgg tgtcctgatc atgctgcgca ggcgctggtg
     3841 cgcaaaggca gacacgccgc cgtcgcgaaa cgggtcctca taccaaaaga agcgcgcgtc
     3901 atcgcaggcg cgacccacat acagcgcatc ggcaaacgta cgcagctcac aggcgggatc
     3961 tagcatcagc gtcatcctgt cgccgacggc tttggcgacg tggagcacgt tctccgcctc
     4021 ttcacgggca ttaccttcat tccagccatg aatcttgaac gccctgtacc cgatgccatg
     4081 gcaggcctca gcgaagtctg cgaacgcttc cttggacgag agcccgcccg ctcggtcacc
     4141 gtgataggtg ctggcgtagg caggcaaacg cttgcggtag ccgcccagca gttcggagac
     4201 cgaagcgtta tagtggcggc ccgcccagtc ccacagcgcg atgtcgattg cgccgtggcc
     4261 catgtggtcg aactggcgaa tgtcgcgctt caggtcttca tagatcgcga ttcgctggcg
     4321 cgcatccttg ccgatcagtc gcggcgccag catcagtgtc tgacccagag cagccggact
     4381 ggacacccag agggcaacat agtcgccgcg gccaccgtcg tcggtatgga tggacaccgc
     4441 atatttgccg agccgggttg tcgccccttt cttgtaggtg acggactgcg aggctccatc
     4501 gcgcgccaga ttggtcgcgt cgaattcaaa ctcgtgtacg tcgacacgga cgatggtgct
     4561 catcatgctc ggtcggagtt ttgcaggttt acttaaacat ctcttcgaca gcgatatgcc
     4621 gatagatatt gttgaagttg aagttgccgt tcttgagacg ggtcttgtcc ctggcatcga
     4681 accatagcgc cgtgcgaagg ctgatgtcga aatggcaatt catgaaacga atgtaggggg
     4741 gtgacccata ggttgtcaat agagtgtttc ccataatgtg atcacccata cgactcttgt
     4801 gacaaaccct tgagccaccg acctggagac aaacccatgg ctaccaccgc caaaaccgtg
     4861 ctcaaccgcc gtttttttaa ttgcgctgga ccagcaccgc tccttgcgca aagcggcagg
     4921 tgatctgtcg atgacgcaat cggccgccag caaagcgctg atggaaattg aaagtgtcat
     4981 gggcggctca ctgttcgagc gctcacgcgt
//
LOCUS       DASUIV010000262        13354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1090582, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000262 DASUIV010000000
VERSION     DASUIV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 13354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090582"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFW59_09445"
     CDS             complement(<1..281)
                     /locus_tag="VFW59_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5364483.1"
                     /translation="MSFLPFSKPTIDEATIAAVGDVLRSGWITSGPKVAEFEKQLSRY
                     FGGRPVRTFNSGTCTMEIALRIAGIGAGDEVITTSISWVATANVILEVGA"
     gene            complement(286..657)
                     /locus_tag="VFW59_09450"
     CDS             complement(286..657)
                     /locus_tag="VFW59_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX5364484.1"
                     /translation="MNFVSLSLIVTGVMFNVAAQLLIKAGTNAVGYFEFSRENILPIG
                     WKLATEPHIIGAMCCYAFGVVIWVLALSRVQVSIAYPLLSLGYVVNAVAAWYLFNEGF
                     NPAKVVGMGIIILGVVIISRA"
     gene            complement(654..2294)
                     /locus_tag="VFW59_09455"
     CDS             complement(654..2294)
                     /locus_tag="VFW59_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5364485.1"
                     /translation="MIASRRSLLILLAVLAVIWFGNLEYRTLVKPDEGRYAEIPREMV
                     ASGDWTTPRLNELKYFEKPPLQYWATATAYTVFGEHQWTSRLWTGLTGFAGILLVWFA
                     GVHLFGREAAGYAALLLSSSLLYVLMGHINTLDMGVTFFITLGIFGLLLAQAQSDVKK
                     RRNWMLLAWAGMALAVLSKGLMGIILPGTAVFIYCMVQRDFTLLKRMHWLAGLAVFAV
                     ITVPWFYLVMKANPEFFERFFIYEHYTRFTTKDLGRYQPWYYFIPILLAGALPWTALM
                     FDSMWRTVRHSHLPEKMFNTQRFLLIWAVFIYVFFSVSGSKLPSYLLPMFPVLALLMG
                     KRIAEMRERVLFWQMTPSIPFALVVLALALNMDKLADTPNQAALYPHYTIWLVASALV
                     TLVGVLSGMALLWRAKKRPAVLLLSLTALLSAQIALTGYESIARERSAKHIAEAIHNE
                     VKPNVPFYSVLTYEQTLPFYLKRTFTLVEYEDEMAYGIQQEPQRRIPTLAEFARVWRQ
                     QPEALGIVPTQFYPLLKQLNLPMQIIFEDTQHIVFKKP"
     gene            complement(2291..3160)
                     /locus_tag="VFW59_09460"
     CDS             complement(2291..3160)
                     /locus_tag="VFW59_09460"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5364486.1"
                     /translation="MKFCLVCKKTFGDASWKCPACGHEPIERGGFVSFAPDLAALNDG
                     FDPALFQRYALVEAGNFWFRARNALLKTLMQRHFPQAANILEIGCGTGFVLANTRATY
                     PDAQLSGSDIFTEGLGFAQQRVPSASLFQMDATAIPFRDEFDLIGAFDVLEHIEEDAV
                     ALAQIHAACKPGGGVIFTVPQHPSLWSRMDEYAHHKRRYTRIELLNKVSAAGFKVRYV
                     TSFVSLLLPAMWLSRVLQRNAPPVADGMDSGLKMHPLVNAVFGAVMAVERGLLALGIT
                     FPAGGSLLVVAEK"
     gene            complement(3180..4052)
                     /locus_tag="VFW59_09465"
     CDS             complement(3180..4052)
                     /locus_tag="VFW59_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX5364487.1"
                     /translation="MSEPQFKKIVVFGVGLIGGSFSLALRRAGAVAEVVGFGRSQATL
                     EQARQLGILDRIGQDVAVEVRDADLVLLATPVGQMAELMARIAPHLGAHTLVTDGGST
                     KSDVVAAARANLGDRIAQFVPAHPIAGAEQSGAAAARAELYQGKKVVLTPLPENSPAS
                     VARVRLAWEACGAVVGELTPQQHDEVFAAVSHLPHLLSFALVHDLAQRENRDLLLSFA
                     ASGFRDFTRIAASSPEMWRDICLANRAALLGELGRYMAELENIHEALADADADKLEEI
                     FSLARKVRSNWQPE"
     gene            complement(4146..5162)
                     /gene="aroF"
                     /locus_tag="VFW59_09470"
     CDS             complement(4146..5162)
                     /gene="aroF"
                     /locus_tag="VFW59_09470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5364488.1"
                     /translation="MIIVMGREVTDEQIGAVVKKLESAGLKANISRGIERTVIGAIGD
                     ERQLTEEMFTPLPGVESAMHIAKQYKIVSRESHRENTVIDIAGVPLGGNQIQIIAGPC
                     SVETQEQMDLSAQFVHEAGCRLMRGGAFKPRTSPYAFQGKGVEGLDIFRKAAVKYKLP
                     IVTELMDARQLDTFLEYDVDVIQIGTRNMQNFDLLKEVGRCNKPIILKRGMSATISEW
                     LMSAEYIAAGGNHNIIFCERGIRTFETAYRNVLDVTAIPVLKKETHLPVIVDPSHAGG
                     KAWMVAALSQAAVAAGADGLLVEMHPSPCDAWCDADQALTPQELKNLMGTLGGIAKAI
                     GRTL"
     gene            complement(5159..6262)
                     /gene="hisC"
                     /locus_tag="VFW59_09475"
     CDS             complement(5159..6262)
                     /gene="hisC"
                     /locus_tag="VFW59_09475"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5364489.1"
                     /translation="MNYSDLAPPYIRAIAPYQPGKPISELERELGITGIVKLASNENP
                     LGASPAAVAAARAALDEIGLYPDGNGFALKDALVQRYGVAHNQVVLGNGSNDLLELSA
                     RAFLTVGDKAVYSAHAFAVYALATQAVGAVGITVPAQDFGHDLDAMRATAVEQGAKMV
                     FIANPNNPTGTFLSGAALHDFMSKLPANILVILDEAYNEYLPEECRYDSVSWLSEFPN
                     LIISRTFSKAYGLASLRVGYALSSAQVIDMINRVRQPFNVNSMAQAAAVAALQDADFV
                     RQTFELNRRGMAQIVAGLEKLGLEHIPSYGNFVSFKITGAMAVYRRLLELGVIVRPIA
                     NYDMPDWLRVSIGLETENQKFLSALTQILGEKQ"
     gene            complement(6294..7358)
                     /gene="pheA"
                     /locus_tag="VFW59_09480"
     CDS             complement(6294..7358)
                     /gene="pheA"
                     /locus_tag="VFW59_09480"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX5364490.1"
                     /translation="MSENLKKYREQIDNLDEELLRLVNQRAALAQQIGHQKQDGTVLR
                     PEREAQVLRRLQALNTGPLSNAAVAALFTEVMSQCRALEAPMTVAYLGPEGTFTEAAA
                     LKRFGSAVQGQPCATIDEVFHAVENDSVQYGTVPVENSTEGAVGRTLDLLQQSSLLVC
                     GEVMLAIHQCLLAQHCDWSLIKTVYSHPQSLGQCQNWLNAKLPHAARIPVASNAEAAR
                     LAAENPSSAAIAGERAAAVFGLSVCVENIEDDARNTTRFLVLSKQRVAPSGHDKTSLV
                     LSAANRPGAVHELLSPLAKHGVSMTKLESRPARSGSWEYVFYVDIEGHQDDAHVAAAL
                     TELKQVATFVKVLGSYPVAV"
     gene            complement(7355..8545)
                     /locus_tag="VFW59_09485"
     CDS             complement(7355..8545)
                     /locus_tag="VFW59_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5364491.1"
                     /translation="MAKTFQVLTLNKIAAAGLNRLPAARYVTGSEIAEPDAIMVRSHN
                     MHEMNIPRSVLAIARAGAGTNNVPVKQMSERGVPVFNAPGANANAVKELVITGMLLAS
                     RNIVPALHFVSSLKGDDETLHKLVEDGKKHYAGTELRGRTLGIVGLGAIGRQVADAAI
                     GLGMQVIGYDPDISVEAAWSLSAQVKKAHSVEDLLRHSDFVTLHVPLLDATRNLIDQN
                     RVSLMKAGAVLLNFSRDAIVNEDAVLSGLERKHLRGYVCDFPSNKTLGHPGIVALPHL
                     GASTQEAEENCAVMVADQLRDYLEHGNVVNTVNFPNVSMPRESACRMAVANANVPNML
                     GQISTALAAAGINIHNMMNKSRGEMAYTLVDTDSAPPAGLIAQIAAIPGVLMVRDLPL
                     LKEL"
     gene            complement(8538..9620)
                     /gene="serC"
                     /locus_tag="VFW59_09490"
     CDS             complement(8538..9620)
                     /gene="serC"
                     /locus_tag="VFW59_09490"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX5364492.1"
                     /translation="MTIYNFSAGPAVLPKEVLQQAQAEMLDWHGSGMSVMEMSHRGKE
                     YMGIHAQAEADLRELMGIPANYKVLFLQGGAHLQFSMIPLNLLRGKASADYVNTGEWS
                     KKAIGEAKKFGSVNVAANNADKNCSYVPAFDTWTRDPNAAYLHYTPNETIGGVEFGWV
                     PATGDVPLVADMSSNILSRTYDVSKFGLIYAGAQKNIGPAGLTLVIVRDDLIGQVPAG
                     TPTMLDYQIHADNDSMYNTPPTYGIYMAGLVFQWLKKNGGIAAMEKNNIAKANLLYAA
                     IDASNGFYSCPVNKADRSRMNVPFTLKDAALDSDFLKQAEARGLLQLKGHRSVGGMRA
                     SIYNAMPIEGVQALAGFMNEFAKKHG"
     gene            complement(9715..12324)
                     /gene="gyrA"
                     /locus_tag="VFW59_09495"
     CDS             complement(9715..12324)
                     /gene="gyrA"
                     /locus_tag="VFW59_09495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5364493.1"
                     /translation="MDQFAKETLPVSLEEEMRKSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELSNDWNRAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQDFSLRYTL
                     VDGQGNFGSVDGDNAAAMRYTEIRMSRIAHELLADLDKETVDYGPNYDGSEHEPLVFP
                     ARFPNLLVNGSSGIAVGMATNIPPHNLNEVVDACLALLNNPDMDIEELIELVPAPDFP
                     TAGFIYGLSGVREGYRTGRGRVVMRGRTHFENIGKTDREAIIIDELPYQVNKANLLIK
                     IGELVREKRLEGISEIRDESDKSGMRAVIELKRGENADVILNKLYKDTQLQDSFGINM
                     VAIVDGQPRLLNLKQVIDAFLRHRREVVTRRTIFELRKARERGHILEGLAVALSNVDE
                     IIALIKAAPAPADAKRELMACVWRSQLVEDMLSRVSDASRPDGLAPEYGLVGQGNDRG
                     YHLSEAQAQAILELRLQRLTGLEQDKIVGEYREVMDKIADLLDILAKPARVTQIIGDE
                     LVAVKAQFGDKRRSEIITHTHDMSMEDLIAPEDVVVTLSHGGYMKAQKLDEYSAQKRG
                     GRGKQAASTKEDDFVDNLFIANTHDYILCFSNRGRAYWLKVYEVPQGSRIARGRPIVN
                     LLPLAEGEKINAILPVREFAEDKYVLFATSDGTVKKTALSQFGNPRKAGIIAIALDEG
                     DFLIGVAITDGHHDVMLFSDEGKAVRFDENDVRVLGRDTRGVRGMNLAKGGKVISLLV
                     AEDEEQTVLTATENGYGKRTPIAEYTRHGRGTQGMIAIQTTERNGKVVAATLVNSDDE
                     IMLIGTNGVMIRTRVKEIREMSRATQGVTLINLDKGDKLAGLSRIAESEEEGAEAQEG
                     LDGAEGQEGEAQE"
     gene            12514..13167
                     /locus_tag="VFW59_09500"
     CDS             12514..13167
                     /locus_tag="VFW59_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5364494.1"
                     /translation="MNRIQQVVSSVCLFSFAQLAAAHPTSIGYLYDSQGTVVHAGSGG
                     CIRTAEWTAANALPECDTVAKPAPVAAAPSAPKTLIKPAAVQAAPSTIRIELSADESF
                     ETGKAELRAETKTKLNRLVMELKDLDYDLIVITGHADRTGSAAANQRLSERRAIAAHN
                     YLVSEGIPANKMQSSGKGSSQPVTKPDDCAGLQHKPLAACLAPDRRMEIRISGTRTK"
     gene            13164..>13354
                     /locus_tag="VFW59_09505"
     CDS             13164..>13354
                     /locus_tag="VFW59_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364495.1"
                     /translation="MTTMLHDRPAASTVASHSRLTKPADDAGKSSVNVDPVELEKFSQ
                     LAHKWWDPNSEFKPLHDINP"
BASE COUNT         2563 a         4375 c         3843 g         2573 t
ORIGIN      
        1 gcccccactt cgaggatcac gttcgccgtt gcgacccagg agatagacgt ggtgatgact
       61 tcgtcccctg ccccgatacc ggcgatgcgc agcgcgatct ccatcgtgca ggtgccggaa
      121 ttgaaggtgc gcaccgggcg accgccgaag tacctggaca gttgcttttc aaattccgcc
      181 accttcggcc cgctggtgat ccagccggag cgcagcacat cgcctactgc ggcgatggtc
      241 gcttcatcta tggtcggttt ggaaaaagga aggaagctca tgtttttatg ctctcgaaat
      301 gatgacgacg ccgaggatga tgatgcccat gccgaccacc ttggccggat tgaaaccctc
      361 gttgaacaga taccaggccg ctaccgcgtt caccacatag cccagcgaca gcagcggata
      421 agcgatgctg acctgcaccc gcgacagcgc gagcacccag atcaccacgc cgaaggcata
      481 gcagcacatc gcgccgatga tgtgcggttc ggtcgccagc ttccagccta tcggcagaat
      541 attctcgcgc gagaactcga agtagccgac cgcgttggtg cctgccttga tcagcaattg
      601 cgcggcgacg ttgaacatca ccccggtgac gatgagactg aggctgacga aattcatggt
      661 tttttgaaca cgatatgttg ggtatcttcg aagatgatct gcatcggcag attgagttgt
      721 ttcaacagcg gatagaactg agtcggcacg atgcccagcg cctcgggctg ctgacgccag
      781 accctggcaa actcggccag ggtcggtatc cggcgctgcg gctcctgctg gatgccgtaa
      841 gccatctcgt cctcgtactc caccagtgtg aaggtgcgtt tcagatagaa cggcagggtc
      901 tgctcgtagg tcagcaccga atagaacggc acattcggtt tcacttcgtt atgaatagcc
      961 tcggcgatat gcttggccga gcgttcgcgc gcgatgctct cgtaaccggt cagggcgatc
     1021 tgtgcggaca gcagtgccgt caatgacagc agcagcaccg ccgggcgctt cttcgcgcgc
     1081 cacagcagtg ccatgccgga cagcacgcca accagcgtga ccagagcgct tgccaccagc
     1141 cagatggtgt aatgcggata gagcgcggcc tgattcggcg tgtcggccag cttgtccata
     1201 ttcagcgcca atgccagcac caccaatgca aacggtatcg acggcgtcat ctgccagaac
     1261 aacacgcgct cgcgcatctc ggcgatgcgc ttgcccatca gcaaggccag caccggaaac
     1321 atcggcagca gataggacgg cagcttggag cccgataccg agaagaacac atagatgaac
     1381 accgcccaga tcagcaggaa acgctgggtg ttgaacatct tctccggcag atgactgtga
     1441 cgcaccgtcc gccacatgct gtcgaacatc agcgccgtcc agggcagcgc ccccgccagc
     1501 aggatgggga tgaagtaata ccagggctga tagcgcccca gatccttggt ggtaaatctc
     1561 gtgtagtgtt cgtagatgaa gaagcgctcg aagaactccg ggttggcctt catcaccaga
     1621 tagaaccagg gcacggtgat gaccgcaaat accgcgagcc cggccagcca gtgcatgcgt
     1681 ttgagcaagg taaaatcgcg ctgcaccatg caatagatga acaccgccgt gcccggcagg
     1741 atgatgccca tcaagccctt gctcaacacc gccagcgcca tgcccgccca ggcgagcaac
     1801 atccagttgc gccgcttctt cacatcggac tgagcctgtg ccagcaacag tccgaagatg
     1861 cccagcgtga tgaagaaggt cacgcccatg tccagcgtat tgatgtggcc catcagcaca
     1921 tacagcagac tgctggacaa cagcagcgcg gcatagcccg ccgcctcgcg cccgaacaga
     1981 tgcaccccgg cgaaccacac cagcaggatg ccggcaaaac cggtcagccc ggtccacagc
     2041 cgcgaagtcc actggtgttc gccgaatacg gtgtaggccg tcgccgtggc ccagtattgc
     2101 agcggcggct tctcgaaata cttcagttcg ttcaaacgcg gcgtcgtcca gtcaccgctt
     2161 gccaccatct cgcgcggaat ctcggcgtaa cggccctcat ccggcttgac cagtgtgcgg
     2221 tattcaagat tgccgaacca gatcaccgcc agtaccgcca gcaggatcaa cagactgcgt
     2281 cgcgaggcga tcatttctcg gccaccacca gcaacgatcc gcctgccggg aaggtgatgc
     2341 ccagcgccag caagccgcgc tctactgcca tcaccgcgcc gaacaccgca ttcaccagag
     2401 gatgcatctt caggcccgaa tccatgccat ccgcaaccgg cggcgcgttg cgctgcaaca
     2461 cgcgcgacag ccacatcgca ggcagcaaca gcgacacgaa cgacgtcaca tagcgcacct
     2521 tgaatcctgc ggcactcacc ttgttcagca actcgatacg ggtgtagcgc cgcttgtgat
     2581 gcgcgtattc gtccatacgg ctccacagcg agggatgctg cggcacggtg aagatcaccc
     2641 ctccgcccgg cttgcaggcg gcatgtatct gcgccagcgc caccgcatcc tcttcgatgt
     2701 gttccagcac gtcaaaggca ccgatcagat cgaactcgtc gcggaacggg atcgcggtcg
     2761 catccatctg gaacaggctg gccgacggca cacgctgctg cgcgaagccc agcccctcgg
     2821 tgaagatatc gctgccggac agctgcgcat ccggataggt cgcgcgcgta ttcgccagca
     2881 cgaagccggt gccgcaaccg atctccagga tgttggctgc ctgcggaaaa tggcgctgca
     2941 tcagcgtctt cagcagcgca ttgcgcgccc tgaaccagaa attgcccgcc tcgaccagcg
     3001 cataacgctg aaacagcgcg ggatcgaagc cgtcgttcaa cgccgcgaga tcgggcgcga
     3061 acgagacgaa accgccgcgc tctatcggct catggccgca agcagggcat ttccagcttg
     3121 catcgccaaa tgttttttta cagaccaggc agaatttcat aatgtgattc gttcgataat
     3181 tattcgggtt gccagttgct gcgcaccttg cgcgccaggc taaaaatttc ttccagcttg
     3241 tcggcatctg cgtcagccag tgcctcatgg atattttcca gctctgccat gtaacgcccc
     3301 agttcgccta acaaggcggc acggttcgcc aggcagatgt cgcgccacat ctcggggctg
     3361 ctcgcggcga tgcgtgtgaa gtcgcgaaag ccgctggcgg caaacgacag caacaggtcg
     3421 cggttttcgc gctgcgccag atcatgcacc agcgcgaacg acagcagatg cggcagatgg
     3481 ctcacggcgg caaacacttc atcatgctgt tgcggcgtca attcgcccac caccgcaccg
     3541 cacgcctccc aggccagcct gacgcgggcc accgatgcgg gcgagttttc cggcaacggg
     3601 gtcagcacca ctttcttgcc ctgatacagc tccgcacgcg ccgctgccgc cccgctctgc
     3661 tcggcgcccg cgatgggatg agccggaacg aactgcgcta tcctgtcgcc cagattcgcg
     3721 cgcgctgcgg cgaccacatc ggatttggtg ctgccgccgt cggtgaccag cgtatgcgcg
     3781 cccagatgcg gtgcgatgcg cgccatcagt tccgccatct gtccgaccgg ggtcgccagc
     3841 aacaccagat cggcgtcacg cacctcaacc gcgacatcct gaccgatgcg gtcgaggatg
     3901 ccgagctgtc tggcctgctc cagcgtcgct tgactgcgac cgaaaccgac gacttccgcg
     3961 accgcaccag cccggcgcag cgccagagag aacgagccgc cgatcaggcc gacgccgaag
     4021 acgacgattt ttttgaattg cggttcggac attccgtccc tctcccctac ccctctcccg
     4081 ccagcgggcg aggggtggac tctcgtaggg tgggttaggc agcaggccgt aacccaccaa
     4141 tcagattaca gcgtgcgccc gatagccttg gcgatgccgc ccagcgtgcc catcaggttc
     4201 ttcagttcct gcggcgtcag cgcctggtcg gcgtcgcacc atgcatcgca ggggctagga
     4261 tgcatctcga ccagcaggcc gtccgcaccg gcggcgaccg ccgcctgcga cagcgccgcg
     4321 accatccatg ccttgccgcc cgcgtgcgaa ggatcgacga tcaccggcag gtgggtctct
     4381 ttcttcagca ccggaatcgc ggtcacgtcc agcacgttgc ggtaagcggt ctcgaaggtg
     4441 cggatgccgc gctcgcagaa gatgatgttg tggttgccgc ccgcagcgat gtattccgcc
     4501 gacatcagcc attcggaaat ggtcgcggac atgccgcgct tgaggatgat cggcttgttg
     4561 cagcggccga cttccttgag cagatcgaag ttctgcatgt tgcgcgtgcc gatctggatc
     4621 acgtccacgt cgtattcgag gaaggtatcc agttggcgcg catccatcag ttcggtgacg
     4681 ataggcagct tgtacttgac cgccgccttg cggaagatgt ccagcccttc cacgcccttg
     4741 ccctggaagg cataggggct ggtgcgcggc ttgaacgcac cgccgcgcat cagacggcaa
     4801 cccgcctcgt gcacgaactg tgcggacaga tccatctgtt cctgcgtttc gaccgaacag
     4861 ggcccggcga tgatctggat ctggttgccg cccagcggca ccccggcgat atcgatcacg
     4921 gtgttctcgc gatgcgactc gcgcgacacg atcttgtact gtttggcgat atgcatcgcc
     4981 gattccacgc ccggcagcgg cgtgaacatc tcttcggtca actggcgctc gtcgccgatc
     5041 gcaccgatca cggtacgctc gatgccgcgc gagatgttgg ccttcagccc ggccgattcc
     5101 agtttcttga cgacggcacc gatctgttca tcggtgactt ctcttcccat tacgataatc
     5161 attgcttctc tccaagtatc tgcgtcagcg cagacaaaaa tttctgattc tctgtttcca
     5221 gaccaatgct cacgcgcagc cagtccggca tgtcgtagtt cgcgatggga cgcacgatca
     5281 cgcccagctc cagcagacgg cgatagaccg ccatcgcccc tgtaattttg aagctcacga
     5341 aattcccata ggacgggatg tgctccagcc ccagcttctc cagccccgcg acgatctgcg
     5401 ccatgccgcg acggttcaac tcgaaagtct ggcgcacgaa gtccgcatcc tgcaatgccg
     5461 ccacggcggc cgcctgcgcc atgctgttga cgttgaacgg ctggcgcacg cggttgatca
     5521 tgtcgatcac ctgcgcactg gacaaggcat aaccgacgcg caggctcgcc agaccatagg
     5581 ccttggagaa ggtgcgcgaa atgatcagat tggggaactc gctcagccag ctcacgctgt
     5641 cgtagcggca ctcttccggc agatattcgt tgtaggcctc gtccagtatc accagaatgt
     5701 tcgccggcag cttgctcatg aaatcgtgca gcgccgcgcc ggacaggaac gttccggtcg
     5761 gattgttcgg gttggcgatg aacaccatct tcgcgccctg ctccaccgca gtcgcgcgca
     5821 tcgcgtcgag gtcgtgaccg aagtcctggg ccggaacggt gatgccgacc gcgcccaccg
     5881 cctgtgtcgc cagtgcatac accgcgaagg catgggcgga atacaccgcc ttgtcgccca
     5941 cggtcaggaa ggcgcgagcg gacagctcca gcagatcgtt cgagccgttg cccagcacga
     6001 cctggttgtg ggccacgccg tagcgctgca ccagcgcgtc tttcagcgcg aaaccgttgc
     6061 cgtccggata caggccgatc tcgtcgagcg cggcacgcgc ggcggcaacg gcggcaggac
     6121 tcgcgcccag cggattctcg ttcgaggcca gcttgacgat accggtgatg cccagctcgc
     6181 gctccagttc ggagatcggt ttgccgggct gatagggtgc tatggcacgg atatagggag
     6241 gtgctaaatc gctgtagttc atttgttgat tacgattact tgaaacttgg atttcagacg
     6301 gcgaccgggt aggaacccag caccttgacg aaggtggcga cctgtttgag ttcggtcaat
     6361 gccgccgcga catgggcatc gtcctgatgc ccctcgatat cgacatagaa cacatactcc
     6421 cagctgcccg aacgcgccgg gcgcgactcc agcttggtca tcgagacgcc gtgcttagcc
     6481 agcggcgaga gcagctcgtg caccgcaccg ggacgatttg cggcagacag caccagcgag
     6541 gtcttgtcgt gaccggaagg tgcgacacgt tgcttgctca gcaccaggaa acgcgtggtg
     6601 ttgcgcgcat cgtcctcgat attctccacg cacacgctca gtccgaagac cgctgcggca
     6661 cgctcgccgg cgatcgccgc gctgctcggg ttctcggcgg ccagacgcgc cgcctcggcg
     6721 ttgctggcga ccggaatgcg cgccgcatgc ggcagcttgg cattcagcca gttctggcat
     6781 tggcccagcg attgcggatg cgaatagacg gtcttgatca ggctccagtc gcaatgttgc
     6841 gccagcaggc actggtggat ggccagcatc acctcgccgc acaccagcaa cgagctttgc
     6901 tgcaacagat ccagcgtgcg tccgaccgcg ccctcggtgg agttctctac cggcaccgtg
     6961 ccatattgca cggagtcgtt ctccaccgca tggaacacct cgtcgatggt cgcacagggc
     7021 tggccctgca ccgcgctgcc gaagcgcttg agcgcggccg cctcggtaaa cgtcccttcc
     7081 ggcccgagat aagccaccgt catcggcgcc tccagcgcgc ggcattgcga catcacctcg
     7141 gtgaacaacg ctgccacggc ggcgttgctc aacgggccgg tattcagcgc ctgcaaacgg
     7201 cgcaacacct gagcctcgcg ctcgggacgc agcaccgtac catcctgctt ctggtgtccg
     7261 atctgttgcg ccagcgcggc gcgctggttg accagcctga gcagctcttc gtccaggtta
     7321 tcgatctgtt cgcggtattt tttcaggttc tcgctcatag ttccttcagc agcggcaagt
     7381 cgcgcaccat cagcacaccg ggaatcgccg cgatctgcgc gatcagtccg gcaggcggtg
     7441 cagagtcggt gtccaccagc gtataggcca tctcgccgcg cgacttgttc atcatgttat
     7501 ggatattgat accggcagca gccagtgcgg tcgaaatctg gcccagcatg ttcggtacat
     7561 tggcattcgc caccgccatg cggcaagcgg actcgcgcgg catgctgacg ttgggaaagt
     7621 tcaccgtatt gaccacgttg ccgtgctcca gatagtcgcg caactgatcg gccaccatca
     7681 ccgcacaatt ttcctcggct tcctgagtgg acgcgcccag atgcggcagc gcgacgatgc
     7741 cgggatgccc cagcgtcttg ttgctcggaa agtcgcacac atagccgcgc agatgcttgc
     7801 gctccaaacc gctcagcacc gcgtcttcgt tgacgatggc atcgcgcgag aagttcagca
     7861 gcaccgcccc cgccttcatc aaagacacgc gattctgatc gatcaggttg cgcgtcgcat
     7921 ccagcagcgg cacatgcagc gtgacgaaat cgctgtgccg gagcagatct tccacgctgt
     7981 gcgctttctt cacctgcgcg gacaggctcc acgccgcttc caccgagata tccggatcgt
     8041 aaccgatcac ctgcatgccc agaccgatgg cggcatcggc cacctggcgc ccgatcgcgc
     8101 cgagaccgac gatgcccagc gtgcggccgc gcaactcggt gcctgcatag tgtttcttgc
     8161 cgtcctcgac cagcttgtgc agggtctcgt catcaccctt caggctcgag acgaaatgca
     8221 gcgcgggcac gatgttgcgc gaagcgagca gcatgccggt gatcaccagt tccttcaccg
     8281 cattggcatt cgcgcccggg gcgttgaaaa ccggcacgcc gcgctcgctc atctgcttca
     8341 ccggcacgtt gttggtgccc gcacccgcac gtgcaatcgc cagcacgctg cgcggaatgt
     8401 tcatctcgtg catgttgtgc gaacgcacca tgatcgcatc cggctcggca atttcgctgc
     8461 ccgtgacata gcgcgctgcg ggcagacgat tcagcccggc ggcggcgatc ttgttcagcg
     8521 tgagaacctg aaaggtctta gccatgcttc ttggcgaact cgttcatgaa accggccagc
     8581 gcctgcacgc cttcgatggg catggcattg tagatcgagg cgcgcatgcc tccgacggaa
     8641 cggtgtccct tcagttgcag caggccgcgt gcctccgcct gcttcaaaaa gtcgctgtcc
     8701 agcgccgcat ccttcagcgt gaagggcacg ttcatccgcg aacggtccgc cttgttcacc
     8761 gggcagctgt agaaaccgtt gctcgcatcg atggccgcat acagcagatt cgccttggcg
     8821 atgttgttct tctccatcgc ggcaatgccg ccgttcttct tcagccactg gaataccaga
     8881 ccggccatgt agatgccgta ggtcggcggc gtgttgtaca tcgaatcgtt gtcggcatgg
     8941 atctggtagt ccagcatggt cggcgtaccg gcgggcacct gaccgatcag gtcgtcgcgc
     9001 acgatcacca gcgtcagacc ggccgggccg atgttcttct gcgcacccgc atagatcagg
     9061 ccgaacttcg acacatcata agtccgcgac aggatgttcg aggacatgtc cgccaccagc
     9121 ggcacgtcgc cagtcgccgg cacccagccg aactccacgc cgccgatggt ctcgttcggg
     9181 gtgtagtgca gataggccgc gttcggatcg cgcgtccagg tatcgaatgc cggaacgtag
     9241 ctgcagttct tgtcggcgtt gttcgccgcc acgttcacgc tgccgaattt cttcgcctcg
     9301 ccgatggcct tcttcgacca ctcgccggta ttgacatagt cggcggaagc cttgccgcgc
     9361 aacaggttca acgggatcat cgagaattgc agatgcgcgc cgccttgcag gaacagcacc
     9421 ttgtaattgg cagggatgcc catcagttcg cgcagatcgg cttcggcctg ggcatggatg
     9481 cccatgtatt ccttgccgcg atgcgacatc tccatcaccg acataccgct gccgtgccag
     9541 tcgagcattt ccgcctgtgc ctgctgcaac acctctttcg gcaacaccgc cggacccgcg
     9601 ctgaagttat agatcgtcat gctgttcaat ctccctggct gtttagtgac tcatacttga
     9661 aagattgagg atcgagtgag aagcggtgca ccgagttttc tcgatcctct atccttattc
     9721 ctgagcttcc ccttcttgac cctcggcccc gtccaatcct tcttgagcct ctgccccttc
     9781 ttcttcggac tcggcgatgc ggctcaaacc ggcgagcttg tcgcccttgt ccagattgat
     9841 cagcgtcacg ccctgggtcg cacggctcat ctcgcggatc tccttgacgc gggtgcggat
     9901 catcacgcca ttggtgccga tcagcatgat ctcgtcatcg ctgttcacca gcgttgccgc
     9961 caccaccttg ccgttacgct ccgtggtctg gatcgcgatc atgccctgcg tgccgcgccc
    10021 gtgacgggtg tattcggcga taggcgtacg cttgccgtaa ccgttttcgg tcgcggtcag
    10081 cacggtctgc tcttcatcct ccgccaccag cagcgagatc accttgccgc ccttggccag
    10141 attcatgccg cgcacgccgc gcgtatcgcg gcccagcacg cgcacgtcgt tctcgtcgaa
    10201 gcgcaccgcc ttgccctcgt cggagaacag catcacgtcg tgatgcccgt cggtgatcgc
    10261 cacgccgatc aggaagtcgc cctcgtccag cgcgatggcg atgatgcccg ccttgcgcgg
    10321 attgccgaac tgcgacagtg cggtcttctt caccgtgccg tcggaggtgg cgaacagcac
    10381 atatttgtct tccgcgaatt cgcgcaccgg caggatcgcg ttgatcttct cgccttcggc
    10441 cagcggcaac aggttcacga taggcctgcc gcgcgcgata cggctgccct gcggcacttc
    10501 atataccttc agccagtacg cgcggccacg gttggagaag cacaggatgt agtcatgcgt
    10561 attggcgatg aacaggttgt cgacgaaatc gtcttccttg gtgctggccg cctgcttgcc
    10621 gcgtccgccg cgcttctgcg cgctgtactc gtccagcttc tgcgccttca tgtaaccgcc
    10681 atgcgacagc gtgaccacca cgtcttccgg cgcgatcagg tcttccatgc tcatgtcgtg
    10741 cgtgtgggtg atgatctcgc tgcggcgctt gtcgccgaac tgcgccttca ccgccaccag
    10801 ctcgtcgcca atgatctgcg tgacgcgcgc aggcttagcc agaatgtcca gcaggtcggc
    10861 gatcttgtcc atcacctcgc ggtattcgcc gacgatcttg tcctgctcca ggccggtcag
    10921 gcgttgcaga cgcagctcaa ggatcgcctg agcctgagct tcggaaagat ggtaaccacg
    10981 gtcattgccc tgccccacca gaccatactc cggcgccagt ccgtccggac gcgacgcatc
    11041 gctgacgcgg gacaacatgt cctcgaccag ctgcgaacgc cagacgcacg ccatcagctc
    11101 gcgcttggca tcggccgggg ccggggccgc cttgatcagc gcgatgatct cgtccacgtt
    11161 ggacagcgcg accgccaagc cttccaggat gtggccgcgc tcgcgcgcct tgcgcaattc
    11221 gaaaatggtg cgacgcgtga cgacttcgcg gcgatggcgc aggaaggcgt cgatcacctg
    11281 cttgaggttg agcagtcgcg gctggccgtc cacgatggcg accatgttga tgccgaaact
    11341 gtcctgcaac tgggtgtctt tgtacagttt gttcaggatc acatcggcgt tctcgccgcg
    11401 cttgagctcg atcaccgcgc gcataccgga cttgtccgat tcgtcgcgga tttcggagat
    11461 gccttccagc cgcttctcgc gcaccagctc gccgatcttg atcagcaggt tggccttgtt
    11521 cacctgatac ggaagctcat cgatgatgat ggcctcgcga tcggtcttgc cgatattctc
    11581 gaagtgggtg cgtccgcgca tcaccacacg gccgcgaccg gtgcgataac cttcacgcac
    11641 gcccgacagg ccgtagatga aacccgcagt cgggaagtcc ggtgcgggca ccagctcgat
    11701 cagctcctcg atatccatgt ccggattatt caacagcgcc agacaggcat ccaccacctc
    11761 gttcaggttg tgcggcggaa tattggtcgc catgcccacc gcgataccgg aggaaccgtt
    11821 caccagcagg ttggggaagc gcgccgggaa caccagcggc tcatgctccg aaccgtcgta
    11881 gttcgggccg taatcgaccg tctccttgtc cagatcggcc agcagttcgt gcgcgatgcg
    11941 cgacatgcgg atctcggtgt aacgcatcgc cgccgcgtta tcgccgtcca ccgaaccgaa
    12001 gttaccctga ccgtccacca gcgtgtagcg cagcgaaaaa tcctgcgcca tgcggacgat
    12061 ggtgtcgtac accgccgtgt cgccgtgcgg gtggtattta ccgatcacgt cgccgacgat
    12121 acgcgccgac ttcttgtagg cgcggttcca gtcgttggac agttcgtgca tcgcaaacag
    12181 cacgcgccgg tgtacgggct tgaggccgtc gcgcacatcg ggcaatgctc gcccgacgat
    12241 aacgctcatc gcgtaatcga gatacgactt gcgcatctcc tcttctagac taactggcag
    12301 ggtttcttta gcgaattgat ccatagtatc gggtgggtac ggtttagctt gaaaacagcg
    12361 cgtgagttta ccacgcagcc cgcgccggag acaaaactga ttaccgggcg ttttttaagc
    12421 aacaatccac cgatcggcca tttgcaacaa aactggctag ccattaccat ccctcacctc
    12481 caaatcaaaa cctcaaaaca aacaggggcc gtcatgaatc gaattcagca agtcgtctcg
    12541 tcagtctgcc tattcagctt cgcgcagctc gctgcagcac acccgaccag catcggctac
    12601 ctgtatgact ctcagggaac cgtggtacat gcgggttccg gtggctgcat ccgcaccgca
    12661 gaatggactg cggccaatgc cctgcctgaa tgcgatacgg tcgccaaacc ggctccggtc
    12721 gctgccgcac cgtcggcccc caagacactg atcaaacccg ctgccgtgca ggccgctccc
    12781 tccaccatca ggatcgaact gagcgccgac gagtcgttcg aaaccggcaa ggccgaactc
    12841 cgagccgaaa ccaagaccaa gctcaatcgg ctggtcatgg aattgaaaga cctagactat
    12901 gacctgatcg tcatcaccgg ccatgccgac cgcaccggtt cggctgcggc caaccagcgc
    12961 ctgtccgagc gccgcgccat tgccgcacac aactatctgg tcagcgaagg catccctgcc
    13021 aacaagatgc agagcagcgg caagggcagc agccagccgg tcaccaagcc agacgactgc
    13081 gcgggactgc agcacaagcc gctggccgcc tgtctggcac cggaccgccg catggaaatc
    13141 cgcatctcgg gtacacgcac caaatgacga caatgctgca tgaccgtccc gcagcttcca
    13201 ccgtggcttc gcatagccgt ctgactaagc cagcagacga cgctggcaag tcatcggtta
    13261 atgtcgatcc ggtggagctg gaaaaattct cgcagctggc gcacaagtgg tgggatccga
    13321 acagcgaatt caaaccgctg cacgacatca accc
//
LOCUS       DASUIV010000263        10762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_254101, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000263 DASUIV010000000
VERSION     DASUIV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254101"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFW59_09510"
     CDS             complement(<1..701)
                     /locus_tag="VFW59_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX5364496.1"
                     /translation="MKSSEIRQKFLDFFASKGHAIVPSSSLVPHEDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRATSSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     HDAIKYAWELLTEVFKLPKDRLYVTVYAEDDEAYDIWTKEMGLAPERVIRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGEHIPGGLPGTPEEDGDRYIEIWNNVFMQFNRD
                     EAGVMHPLPKPSVDTG"
     gene            complement(829..1752)
                     /locus_tag="VFW59_09515"
     CDS             complement(829..1752)
                     /locus_tag="VFW59_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX5364497.1"
                     /translation="MHTAYITHPLCLKHDMGRDHPESPARIHAIEDQLIASGVLDHLQ
                     RHDAPEATREQLQRVHDAAYLDAIEASAPQQGRVQLDGDTAMNPFSYPAALRAAGAAV
                     LGVDLVMAGEVENVFCNIRPPGHHAERGRAMGFCIFNNVAVAAAHALTQHGLQRVAIA
                     DFDVHHGNGTEHIFSDEPRVMLCSTFQHPFYPYCGADSGNEHIVNVPLPAGSGSEAFR
                     DAVTQHWLPALEAFQPELLLISAGFDAHRDDDMSMLAFTDADYAWVTGVLKEVAAKHA
                     RRRIVSVLEGGYELPALGRSAMAHIKVLASL"
     gene            1819..4497
                     /locus_tag="VFW59_09520"
     CDS             1819..4497
                     /locus_tag="VFW59_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEX5364498.1"
                     /translation="MGKHYLNTLFAPKSVAVFGASERPDAVGQIVFKNMLDSGFQGKL
                     YPINPKSPLVQGQQAYTSIAEIGEPVELVVIATPPKTVPGIIEECGIHGVKAAVIITA
                     GFGEAGPSGIALEKQLIETARRYGIRLVGPNCLGIMRPSIGLNATFNKGGANTGNIAF
                     VSQSGALCTAILDWAQSNDVGFSSVVSMGSSTDVDFGEILDYLVSDANTTSILMYIEG
                     IRNARSFMSAIRAAARIKPVILVKVGRHAAGSKAAMSHTASLVGADDVFDAAVSRAGV
                     VRVQTITQLFTAAKALSCGFRPVGNRLAIVTNGGGPGVMATDRAADLGLAMATLSEGT
                     IEYLNQHLPPNWPHGNPVDIIGDAQADRYHHAVKACLEDDNVDGVLAILTPQAMTKPL
                     ESAQALIDLSNNHSKPLLTCWMGETQVAEAREAFSGAHRPHFRTPEPAVEVFSHLSAY
                     YRNQKLLMQMPGPFAHHIAPDVESARLIIEGAMQEHRKVLTEMESKALLSAFHIPVAK
                     TMVAHSPNEALLIAQQLGFPVAMKVNSHDITHKSDAGGVMLNLGNAHEVRAAYQHIHD
                     NIQRNRPNARMDGISIEPMIVKPNGRELMIGVTCDPVFGPVITFGAGGTAVEIMGDRA
                     VALPPLNNFLVKELIQDTRISKMLGAFRNMAPANMEALEDVLLRVSEMVCELPLLKEM
                     DINPLILDEHGALAADARVVVEYRQPSADRYAHMAIYPYPTQLVSQWQLADGTDITIR
                     PIRPEDAELVQAFVHNLSEESKYFRFMNSMQELTETMLVRFTQLDYSREMALIAVTQE
                     QEQEVELGVARYTINPDGDSCEFALVVADNITGKGLGQKLMVSLMEAARASGLKTIEG
                     EVLKNNHNMIKLINRLGFSSTTSDQDQGIIKISKPL"
     gene            4513..4869
                     /locus_tag="VFW59_09525"
     CDS             4513..4869
                     /locus_tag="VFW59_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEX5364499.1"
                     /translation="MPDQPDLLMLRDRLRSFAEERDWEPFHTPKNLSMALMVEVAELM
                     EHFQWLTAEQSAELPAETRQAVSEELADCLLYLVRLSDRLGVDLRAAALLKLEKNALK
                     YPADRVRGSAKKYSDY"
     gene            4964..6049
                     /locus_tag="VFW59_09530"
     CDS             4964..6049
                     /locus_tag="VFW59_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5364500.1"
                     /translation="MARLELRNVTRRFGDFVAVNDVSLTIESGEFFTLLGPSGCGKTT
                     ILRMIAGFDLPDSGQILLDGHDLADTLPEQRPVHTVFQSYALFPHMTVAGNVAFPLKM
                     AHRPEDEIHQRTREALEQVRLADKASAYPHELSGGQKQRVALARGLINRPRLLLLDES
                     LAALDAKLREEMQVELIKLQREVGITFVFVTHAQVEALALSHRIAVMSQGKVEQVDEP
                     SKLYGFPRNRFVADFIGHINTLPAQIIESTHGHLRLNMAELGEIVAPPLPGAAAGDKG
                     TFAIRPEQVRIGLHSEFDELKNHFSGEIHSLLYQGDVSVYRVRLVNGAVIESLLPNSA
                     PGRAELFEVGDAVKVAWRHDAGMFLRE"
     gene            6042..6950
                     /locus_tag="VFW59_09535"
     CDS             6042..6950
                     /locus_tag="VFW59_09535"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5364501.1"
                     /translation="MSDEPKSARATWLTKWLVSGPPLLFLLLLVAAPSAITVLASFRY
                     PGEFGGLAPLAAADSSALQGLTLETYAFFFSDFIYTEIFIKSFFMALATTLLCLLLAY
                     PLALLIARSPKRNRNLMVLLVALPFTSNFLIGIYAWMIILGPQAAFSQLLNGTLAVFG
                     IAPVRILFSPFAVLIAMVYVHLPFMALPLYTNLEKHDPNLLDAAQDLGASAWQRFWRV
                     TFPLSLPGILAGSALVFIPALGMFAIPEMLGGTGDILIGNLIKEQFLGTRDWPFGSTL
                     SIMLTIAVLLAVWLAARLGGRGRHYV"
     gene            7091..7867
                     /locus_tag="VFW59_09540"
     CDS             7091..7867
                     /locus_tag="VFW59_09540"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5364502.1"
                     /translation="MRRGWLLWTAALTGYAFLYLPLTVVVAYSFNDSRLNAEWVGFTW
                     HWYQVLLHDEEMLLAAGNSLFIALVSSLFATLLGTMAGIALHRYDLKLLNIMVLTPVA
                     MPEILLGVSLLLFFIQVLNLTLGMLSIVLAHITFSIGFVAVIVRSRLAGMDESLFEAA
                     RDLGATPWNTFRYITFPLILPAVIAGGLMAFTLSIDDFVITFFTAGVGVQTLPLRIYS
                     MIKIAVTPEVNAVSTLLMLLTLAMIALASRFAPEVLKGKA"
     gene            7864..8919
                     /locus_tag="VFW59_09545"
     CDS             7864..8919
                     /locus_tag="VFW59_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5364503.1"
                     /translation="MIWRALLLLLWIGHAQAADELHLYNWNNYISEATIARFEEQCAC
                     HVVQDYYSDNEEMLAKLAAGASGYDLIVPTGNAVESLIRQRALRPLDKRLLPNLRNID
                     PAWLDTAFDPGNRYSVPYAYTLTLIGYNSERLRGLNLPVDSWAVVFEPRYLEKLKGRI
                     TVLDSQRELIAAALKYLGYSANDTTESHWKQARDLIIRAKPYWAAFNASSYIKELTVG
                     NIWLVHGYSSDIFQADLDAQKAGRDFRILVSIPKEGAVLALDSMVLHKSGPRPDLAHR
                     FIDFMLDGRNSSELTNLIGSGNPNRDARRYILPEIAQNPAVFPDKSRQTRLEMLRDLD
                     RHQRRVLSRIWTEIKLR"
     gene            complement(8929..9768)
                     /locus_tag="VFW59_09550"
     CDS             complement(8929..9768)
                     /locus_tag="VFW59_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5364504.1"
                     /translation="MRQAYLNDLCVAVLAVVLLAATPCYAMESALELREIYAQSVDRR
                     LTVPPEELRFYAERLTRELQQAGLEQLPPQYLVLVDRSPQVQALLLFWMSDDGVAEFI
                     GASPVSTGRKGGFMYYETPLGVFDHAVANLDFRAEGTENERGIMGYGVKGMRVYDFGW
                     QRARRTWKPGEWDMRLQMHATDPRYLEQRLGTVQSMGCIRIPAALNALFDHYGILDAD
                     YELAMASGQLFWMLRPDREPTRWSGRYLVIIDSYRATRPDWSPLPGTTTRLPRIKPDT
                     VPR"
     gene            9912..>10762
                     /locus_tag="VFW59_09555"
     CDS             9912..>10762
                     /locus_tag="VFW59_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5364505.1"
                     /translation="MAIIGIDLGTSNSAAAVLRGGRPVIIPSAEGISIGGKAFPSYVA
                     VTADGQVLVGEPARRQASANPEGTASAFKRRMGKRENFHLRGKDYSPEQLSAFLLQKI
                     KRDAEAFLGEPVEKAVVTVPAYFDDNQRAATKDACRIAGLEVVRLVNEPTAASLAYGL
                     DRLGQELRIAVIDLGGGTLDVTIMEFGKGVFEVKATSGDTQLGGTDMNQRIFEHLAER
                     FQMSTGVDVRQDQKAAARLIEAAETAKIELSTSVTTHLSLPYLASVNGEPRHLELDLN
                     RTELERLV"
BASE COUNT         2131 a         3453 c         3186 g         1992 t
ORIGIN      
        1 ccggtgtcca ccgatggctt gggcaacgga tgcatcaccc ccgcctcgtc gcggttgaac
       61 tgcatgaaca cgttgttcca gatttcgatg tagcggtcgc cgtcttcctc gggcgtgccg
      121 ggcaggccgc cggggatgtg ctcgccgtga tcgtagaaga tctcggtgca ggggccgcag
      181 ggaccggtgt cgcccatcat ccagaagttg tcggatgcgt agcgcgcgcc cttgttgtcg
      241 ccgatgcgga tcacgcgctc gggcgccaaa cccatctcct tcgtccagat gtcgtaggct
      301 tcgtcgtcct cggcatacac ggtgacgtag agcctgtcct tcggcagctt gaacacctcg
      361 gtcagcagtt cccacgcgta cttgatcgcg tcgtgcttga agtagtcgcc gaagctgaag
      421 ttgcccagca tctcgaagaa ggtgtgatgg cgcgcggtgt aaccgacgtt ctccagatcg
      481 ttgtgcttgc cgccggcgcg cacgcatttc tggctggagg tggcgcgggt atagggtcgc
      541 ttgtcgaagc ccaggaacac gtccttgaac tgattcatgc ccgcgttggt gaacaacagc
      601 gtcggatctt catgcggaac cagcgaactc gacggaacga tggcatgccc cttggaggcg
      661 aagaagtcta aaaatttctg gcggatctca ctgcttttca tttactcacc aatcacttaa
      721 tgcgtttcga ttcaggccgg tttcgaccgg ccaagacagc gcgcatcata ccatccaacc
      781 acctccggaa tgcctcgaaa agacgccttc cttatgccca attccggttc acagactggc
      841 aagaaccttg atgtgagcca tcgcgctgcg ccccagcgca ggcaattcgt accccccttc
      901 cagcaccgac acgatgcggc gccgcgcatg tttggcggcc acctccttca gcacgccggt
      961 cacccaggcg tagtccgcat cggtgaacgc cagcatggac atgtcgtcgt cccgatgcgc
     1021 atcgaacccc gccgagatca gcaacagctc gggctggaag gcctccagcg caggcagcca
     1081 gtgctgcgtc accgcatcgc gaaaagcctc gctgccggaa cctgcgggca acggcacatt
     1141 gacgatgtgc tcgttgccgc tgtccgcacc gcaataggga tagaagggat gctgaaaggt
     1201 cgaacacagc atcacgcgcg gctcgtcgct gaagatatgt tccgtgccgt tgccgtgatg
     1261 cacgtcgaaa tccgcgatcg ccacgcgctg caagccgtgc tgcgtcagcg catgggccgc
     1321 cgcgaccgcg acgttattga agatgcagaa ccccatcgcc cgcccgcgtt cggcatggtg
     1381 tccgggcgga cggatgttgc agaagacgtt ctccacctcg cccgccatca ccagatcgac
     1441 gcccagcacc gccgcccctg cggcgcgcaa tgcggccgga tagctgaacg ggttcatcgc
     1501 ggtgtcgccg tcgagctgta ccctgccctg ctgtggcgcg ctggcttcga tcgcgtcgag
     1561 gtaggctgca tcgtgaacgc gctgcaactg ttcgcgggtc gcctccggcg cgtcatgccg
     1621 ttgcagatga tccagcacgc ccgaggcgat caactgatcc tcgatcgcat ggatgcgcgc
     1681 aggagactcc ggatgatccc gccccatgtc gtgtttgaga caaagcggat gggtgatgta
     1741 agcggtatgc atgatcgtgg aggctgaaag ctacggtgtt atcataccca acgaattcac
     1801 caccatagcg aaaaaatcat gggaaaacat tacctcaata cccttttcgc ccccaaatcg
     1861 gtcgctgtat tcggcgccag cgagcgcccc gacgcggtcg gtcagatcgt gttcaagaac
     1921 atgctggata gcggttttca gggaaaactc taccccatca accccaagag cccgctggta
     1981 caaggccagc aggcctacac ttccatcgcc gagatcggcg aaccggtcga actggtcgtc
     2041 atcgccaccc cgcccaagac cgtgcccggc atcatcgagg agtgcggcat ccacggcgtg
     2101 aaggcggccg tcatcatcac cgcaggtttc ggcgaggccg gaccatccgg catcgcgctg
     2161 gaaaagcagc tgatagaaac tgcgcggcgc tacggcattc gtctggtcgg cccgaactgt
     2221 ctgggcatca tgcgcccctc catcggcctg aatgccacgt tcaacaaggg cggcgccaac
     2281 accggcaaca tcgccttcgt atcgcaatcc ggcgcgctgt gtaccgcgat cctcgactgg
     2341 gcgcagagca acgacgtcgg cttctccagc gtggtgtcga tgggctcgtc gaccgacgtg
     2401 gatttcggcg agatactcga ctatctggtg tccgacgcca acaccaccag catcctgatg
     2461 tacatcgaag gcatccgcaa tgcgcgcagc ttcatgagcg cgatccgcgc cgccgcgcgc
     2521 atcaagccgg tgattctggt caaggtcggc cgccatgcgg cgggctccaa ggcggcgatg
     2581 tcgcacaccg catcgctggt gggcgcggac gacgtgttcg atgccgccgt cagccgcgcc
     2641 ggtgtggtgc gcgtgcagac catcacccag ttgttcaccg ccgcgaaggc gctgtcctgc
     2701 ggcttccgtc cggtcggcaa ccgtctggcc atcgtcacca acggcggcgg ccccggcgtg
     2761 atggcgacgg accgagccgc cgatctgggg ctggcgatgg ccacgctgtc cgagggcacc
     2821 atcgaatacc tgaatcagca cctgcctccc aattggccgc acggcaatcc ggtggacatc
     2881 atcggcgacg cgcaggcgga tcgctaccac cacgcggtga aggcctgtct cgaagacgac
     2941 aacgtcgacg gcgtgctggc gatcctaacc ccgcaggcga tgaccaagcc gctggagtcc
     3001 gcacaggcgc tgatcgacct gtccaacaat cacagcaaac cgctgctgac ctgctggatg
     3061 ggtgaaacgc aggtggcgga agcgcgcgag gccttctccg gagcgcaccg cccgcacttc
     3121 cgcacgcccg aaccggccgt cgaagtgttc tcgcacctgt ccgcctacta ccgcaaccag
     3181 aagctgctga tgcagatgcc cggcccgttc gcccatcaca tcgcaccgga tgtggaaagc
     3241 gcgcgcctga tcatcgaggg cgcgatgcag gagcatcgca aggtgctgac cgagatggaa
     3301 tccaaggcgc tgctgtcggc cttccatatt cccgtggcca agaccatggt cgcgcactcg
     3361 cccaacgagg cgctgctgat cgcgcaacaa ctgggcttcc cggtggcgat gaaagtcaac
     3421 tcgcacgaca tcacccacaa gagcgatgcg ggcggcgtga tgctcaatct gggcaatgcg
     3481 cacgaggtgc gcgccgctta ccagcatatc cacgacaaca tccagcgcaa ccgtcccaat
     3541 gccaggatgg acggcatttc catcgagccg atgatcgtca aaccgaatgg ccgcgagctg
     3601 atgataggcg tcacctgcga cccggtgttc ggcccggtga tcaccttcgg cgcgggcggc
     3661 accgccgtcg agatcatggg cgaccgcgcg gtcgccctgc cgccgctgaa caacttcctg
     3721 gtcaaggaac tgatccagga cacccgcatc tccaagatgc tcggtgcctt ccgcaacatg
     3781 gcacccgcca atatggaagc gcttgaagac gtgctgctgc gggtttccga aatggtgtgc
     3841 gaactgccgc tgctcaaaga gatggacatc aatccgctga tcctcgacga gcacggtgcg
     3901 ctggctgccg atgcgcgcgt ggtggtggaa tatcgccagc cgagtgccga ccgctacgcg
     3961 cacatggcga tctaccccta cccgacccag ctggtcagcc agtggcaact cgccgacggc
     4021 accgatatca cgatacgtcc gatccgtccg gaagatgcgg agctggtgca ggccttcgtg
     4081 cataacctgt cggaggaatc caagtatttc cgcttcatga acagcatgca ggagctcacc
     4141 gagaccatgc tggtgcgctt cacccagctc gattacagcc gcgagatggc gctgatcgcc
     4201 gtgacgcagg aacaagagca ggaggtcgaa ctgggcgtcg cccgttacac catcaacccc
     4261 gacggcgatt cctgcgagtt cgcgctggtg gtcgcggaca acatcaccgg caagggattg
     4321 ggacaaaagc tcatggtctc gctgatggaa gcggcgcgtg ccagcggcct gaagaccatc
     4381 gagggcgagg tattgaagaa caaccacaac atgatcaaac tcatcaaccg cctcggtttc
     4441 agcagcacga ccagcgatca ggatcagggc atcatcaaaa tcagcaagcc gctttaaccg
     4501 aggagtccgc gcatgcccga tcaacccgac ctgctgatgc tgcgcgaccg gttgcgttcc
     4561 ttcgccgagg aacgcgactg ggagccgttc cacacgccga agaatctgag catggcgctg
     4621 atggtggaag tcgccgaact gatggaacac ttccagtggc tgaccgcaga gcaatccgcc
     4681 gaactccccg ccgaaacccg acaggcggta tccgaagagc tggccgactg cctgctctat
     4741 ctggtgcgcc tgtccgaccg gctgggcgta gacctgcgtg cagccgccct gctcaagctg
     4801 gaaaagaatg cattgaaata tccggcagat cgggtgcgcg gcagcgcaaa gaaatattcg
     4861 gattactgat tcccgcgcaa aaaaacagat ggcttcgcag atcctgccat aacgcaatat
     4921 cggttagagt attgtttacg caggtacgca ggagggattt gacatggctc gtctggaact
     4981 caggaacgtc acgcgtcgtt ttggcgattt cgtcgccgtg aatgacgtca gcctgacgat
     5041 agagtccggc gagttcttca cgctgctcgg cccctccggc tgcggcaaga ccacgatact
     5101 gcggatgatc gccggtttcg acctgccgga ctcgggacag atcctcctcg acgggcatga
     5161 ccttgccgac acgctgccgg aacagcggcc ggtgcatacg gtattccaaa gctatgcgct
     5221 gttcccgcac atgaccgtcg ccggaaatgt cgccttcccg ctgaagatgg cgcaccgtcc
     5281 cgaagacgaa atccaccagc gcacgcgcga ggcgctggag caggtgcgcc ttgccgacaa
     5341 ggcttccgcc tacccgcacg aactatccgg aggacaaaag cagcgcgtcg cgctggcgcg
     5401 cgggctaatc aaccgtccgc gcctgctgct gctcgatgaa tcactggccg cgctcgacgc
     5461 caagctgcgc gaggaaatgc aggtcgagct gattaaactg caacgcgagg tcggcatcac
     5521 cttcgtgttc gtcactcatg ctcaggtcga ggctctggcg ctgtctcatc gcatcgcggt
     5581 aatgagccag ggcaaggtcg agcaggtcga cgagccctcc aagctatatg gatttccacg
     5641 caaccgtttc gtcgccgatt tcatcggcca tatcaacacc ctgcccgcac agataatcga
     5701 gtcgacgcac ggccatctgc gcctgaacat ggcggagctg ggcgagatcg tcgcgccgcc
     5761 gctgccgggc gctgcggccg gagacaaggg cacgttcgcg atcaggccag aacaggtgcg
     5821 catcggatta cacagcgaat tcgacgaatt gaagaaccat ttctcggggg agattcacag
     5881 cctgttgtat cagggcgatg tcagcgtgta ccgggtcagg cttgtcaatg gcgccgtgat
     5941 cgaatccctg ctgccgaatt ccgcaccggg acgcgccgaa ttgttcgaag tgggcgatgc
     6001 ggtcaaggtc gcctggcgcc acgatgccgg gatgttcctg cgtgagtgac gaaccgaaat
     6061 ccgcgcgcgc cacatggctg accaagtggc tggtcagcgg gccgcctttg ctgtttctgt
     6121 tgttgctggt tgccgcaccc agcgcgatca cggtgctcgc gtcgttccgc tatccgggcg
     6181 aattcggcgg actggctccg ctggctgcgg cggattccag cgccctgcag ggactgacgc
     6241 tggaaaccta cgcatttttc ttcagcgact tcatctacac cgaaatattc atcaagtcat
     6301 ttttcatggc gctcgccacc acgctgctct gcctgttgct ggcctacccg ctcgcactgc
     6361 tgattgcgcg cagcccgaag agaaaccgca acctgatggt gctgctggtc gcgctgccgt
     6421 tcaccagcaa ctttctgatc ggcatctatg cctggatgat catcctcggg ccccaggccg
     6481 cattcagcca actgctcaac ggcacgctgg ccgtattcgg catcgcgccc gtgcgaatac
     6541 tgttctcgcc gttcgcggtg ctgatcgcga tggtgtatgt gcatctgccg ttcatggcgc
     6601 tgccgctgta taccaatctg gaaaagcatg acccgaatct gctggacgcg gcacaggatc
     6661 tgggcgctag cgcctggcag cgtttctggc gcgtgacctt cccgctatcg ctgccgggca
     6721 tcctggcagg atcggcactg gtgttcatcc cggcgctggg tatgtttgcg atcccggaga
     6781 tgctcggcgg caccggcgac atcctgatcg gcaacctgat caaggaacag ttcctcggca
     6841 cgcgcgactg gccgttcggc tccacgctgt cgatcatgct gaccatcgcg gtgctgctgg
     6901 cggtgtggct cgccgcgcgg ctcggcggac gcgggaggca ctatgtatga gatgaagcga
     6961 tacaggggaa tcgggctgtg gacggcggcg ctaaccagcg acttggctgg cgccgtgtgc
     7021 cagcctagtc gaatggctat gcaaagtccg aagcattttg ctgaccttct tgtgaaaaga
     7081 gagaaagaaa atgcgtaggg gctggcttct ctggacggcg gcgctgacag ggtacgcctt
     7141 cctctatctg ccgctgacgg tcgtcgtcgc atattcgttc aacgattcgc gcctgaatgc
     7201 cgaatgggta ggcttcacct ggcactggta tcaggtgctg ctgcacgatg aagagatgct
     7261 gctcgcggcg ggcaattcgc tgttcatcgc gctggtgtcc agcctgttcg cgacgctgct
     7321 cggcaccatg gcggggatcg cactacaccg ctacgacctg aaattgctga acatcatggt
     7381 gctgacaccg gtggcgatgc ccgagatcct gctcggcgta tcgctgctgc tgttcttcat
     7441 ccaggtgctg aatctcacgc tgggcatgct gtccatcgtg ctcgcgcaca tcaccttctc
     7501 gatcggcttc gtcgcggtga tcgtccgctc aagactggcg ggcatggacg agagcctgtt
     7561 cgaggcggcg cgcgatctgg gcgccacgcc ctggaacacc ttccgctaca tcaccttccc
     7621 gctgattctg ccagcggtga tcgccggcgg gctgatggcg ttcacgctgt ccatcgacga
     7681 cttcgtgatc accttcttca ctgcgggcgt cggcgtacag acgctgccgc tgcgtatcta
     7741 ctcgatgatc aaaatcgccg tgacgccgga agtgaacgcg gtgtccacgc tgctgatgct
     7801 gctgacgctt gcgatgatcg cgctggcatc gcgcttcgcg cccgaggtat tgaaaggcaa
     7861 ggcatgatct ggcgcgccct gctcctgctg ctgtggatcg gccatgcgca ggccgccgac
     7921 gaactgcacc tgtacaactg gaacaactac atctcggagg ccaccatcgc gcgcttcgag
     7981 gagcaatgcg cctgccacgt ggtgcaggac tattactccg acaacgagga gatgctcgcc
     8041 aagctcgccg caggtgcgag cggctacgac ctgatcgtgc cgaccggcaa cgcggtcgag
     8101 tcgctgatcc gccagcgcgc gctgcgcccg ctcgacaagc gcctgctgcc caatctgcgc
     8161 aacatcgacc ccgcctggct ggacacggca ttcgaccccg gcaatcgcta ttcggtgccc
     8221 tatgcctaca cgctcacgct gatcggctat aacagcgaga ggctgcgcgg actgaacctg
     8281 cccgtcgaca gctgggccgt ggtgttcgag ccgcgttatc tggagaaact gaagggccgc
     8341 atcaccgtgc tcgacagcca gcgcgaactg attgcggcgg cgctgaaata tctgggctac
     8401 tcggcgaacg acaccacgga atcgcactgg aaacaggcac gcgacctgat tattcgcgcc
     8461 aaaccttatt gggccgcgtt caacgcctcc agctatatca aggagctgac cgtcggcaac
     8521 atctggctgg tgcatggcta ttccagcgac attttccagg ccgacctcga tgcgcagaag
     8581 gccggacgcg acttccgcat tctggtcagc atccccaagg aaggcgcggt gctggcgctg
     8641 gattcgatgg tgctgcacaa aagcggcccg cgccccgacc tcgctcaccg cttcatcgat
     8701 ttcatgctag acggacgcaa ctcatccgaa ctgactaacc tgatcggctc cggcaatccc
     8761 aaccgggatg cccggcgata tatcctgccc gagatcgcgc agaatcctgc cgtgttcccc
     8821 gacaaatccc ggcaaaccag gctggagatg ctgcgcgatc tggatcgcca ccagcgccgc
     8881 gtgctgtcgc gtatctggac cgagatcaaa ttgagatgag caggccggtt aacgcggaac
     8941 ggtatccggt ttgattcttg gcaaacgcgt tgtcgtgccc ggcagcggcg accaatccgg
     9001 ccgggtggca cgataggaat cgatgatgac cagataacgt cccgaccagc gtgtaggctc
     9061 gcgatccggg cgcagcatcc agaacaactg gccactggcc atggccagtt cataatccgc
     9121 atcgagtatg ccgtaatgat cgaacagcgc attcagcgcg gccgggatac ggatgcatcc
     9181 catcgactgc acggtgccca gccgttgctc cagatagcga ggatcggtcg catgcatttg
     9241 cagccgcatg tcccattcgc ctggcttcca ggttctgcgc gcccgctgcc agccaaagtc
     9301 gtacacacgc atccccttca caccgtagcc catgatgccc cgctcgttct ccgttccctc
     9361 ggcacgaaag tccagattgg ccacggcgtg atcgaacaca ccgagcggtg tttcgtaata
     9421 catgaatccg cccttccgtc cggtcgatac aggtgatgca ccgatgaatt cggcgacgcc
     9481 gtcatccgac atccagaaca acagcaatgc ctgcacctgc gggctgcgat cgaccagcac
     9541 cagatattgc ggaggaagtt gctccagtcc cgcctgctgc aattcacgag tgagccgttc
     9601 ggcataaaaa cgcaattctt ccggcggcac agtcagtcgg cgatccacgc tctgcgcata
     9661 gatttctctg agctcaagcg ccgattccat ggcgtagcat ggcgtggcgg ccagcagcac
     9721 cacggccaaa accgcgacac ataaatcgtt taaatacgct tgccgcatgg gtaacccgcc
     9781 agactctaaa aatgtgccgt catgtcatat caaaatcagc gaaccctgac tccttttcaa
     9841 ccgcatctat cgctaatatg gtgaccgtaa cgcaaaaacg agctgaataa caagtcgaac
     9901 agggagaact catggctatc atcggtatcg atctcggcac atccaactcc gcagctgccg
     9961 tgttgcgcgg cgggcgcccc gtcatcatcc ccagcgccga aggcatcagc atcggcggca
    10021 aggcattccc cagctatgtt gcagtcaccg ccgacgggca ggtgctggtc ggcgaaccgg
    10081 cgcgtcgcca ggcctcggcc aacccggagg gaacagccag cgcgttcaag cgccggatgg
    10141 gcaagcgcga aaacttccat ctgaggggca aggattactc gcccgaacaa ctctctgcgt
    10201 tcctgctgca gaagatcaag cgcgatgccg aggccttcct cggcgaaccc gtggaaaagg
    10261 ccgtcgtgac ggtgccggcc tatttcgatg acaaccagcg cgccgcgacc aaggacgcct
    10321 gccgcatcgc cggccttgag gtcgtgcggc tggtcaacga acccaccgcc gcatcgctgg
    10381 cctacggcct cgaccgcctc gggcaggagt tgcgcatcgc cgtgatcgac ctgggcggcg
    10441 gtacgctgga cgtgacgatc atggaattcg gcaagggcgt gttcgaggtc aaggcgacca
    10501 gcggcgacac ccagctcggc ggcaccgaca tgaaccagcg catcttcgag catctcgccg
    10561 aacgtttcca gatgtccacc ggcgtggatg tccgtcagga tcaaaaggcg gccgcgcgac
    10621 tgatcgaggc ggctgaaacg gccaagatcg aactatccac cagcgtcacc acccacctca
    10681 gcctgcccta tcttgcctcc gtcaacggcg agccccgcca tctggaactg gacctcaacc
    10741 gcaccgaact tgagcgactg gt
//
LOCUS       DASUIV010000264         5802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_904708, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000264 DASUIV010000000
VERSION     DASUIV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904708"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1707)
                     /gene="glgA"
                     /locus_tag="VFW59_09560"
     CDS             complement(253..1707)
                     /gene="glgA"
                     /locus_tag="VFW59_09560"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX5364506.1"
                     /translation="MSELKVLFATSELAPLIKTGGLADVSGALPAALRALGVDVRVLL
                     PGYTPVLAQLSQHEVIATFEHLPGFPYARLISGDTENGVPLLVLDCPELYQRAGGPYQ
                     DASGQDWTDNARRFALLSRVAAILASDDTPLAWKPDLLHGNDWQTGLAPAYLHLWHGG
                     VPSVMTIHNMAFQGVFPPQMLGELHLPPSSFAIGGVEYYGNLSFLKAGLFYADHITTV
                     SPNYAQEIQQEPLGFGMQGLLAQRRDSLTGILNGIDTDEWNPATDGYLAKKFSAARMT
                     GKAANKQALQERMGLAVEADVPLLGVVSRFTYQKGLDLLLEIAPRLVGLPVQLALLGS
                     GEPQMQDAARELAARYPGKIAAHVGFSEELSHLIEAGSDMFVMPSRFEPCGLNQLYSQ
                     RYGTPPIVHATGGLADSVTDCTDATLKDGSASGFVFSGMTADNLYATIQRAVELYRDQ
                     KKWKALRKNCMAKDFSWEHSAAAYLEVYRKVLGR"
     gene            complement(1830..2810)
                     /gene="glk"
                     /locus_tag="VFW59_09565"
     CDS             complement(1830..2810)
                     /gene="glk"
                     /locus_tag="VFW59_09565"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX5364507.1"
                     /translation="MKNIYLAGDIGGTKTRLALVEVVGTRVKIVHESRYASRDYATFE
                     GLLGEFLAATDAPSRAAFGIAGPVQGRGVQTTNLPWRIDADALQRQFGFGCVLLNDLE
                     ATAFGIPALDDSDLLILQAGVADASGNTAVIAAGTGLGEAGLYWDGQRHQPFATEGGH
                     ASFSPSDELEMTFFLYLKQQFGHVSWERVVSGMGLLSLYQFLCLYRRTAVPDWLMDEM
                     TNGDAAAAIANAAQAGKDELCVEVLQHFVRLYGAEAGNLALKVMSRGGLYLGGGIAPK
                     ILPQLQDGVFLEAFLNKGRMRPLLEAMPVRVILNDRTALLGAALRAAQSA"
     gene            3143..3757
                     /locus_tag="VFW59_09570"
     CDS             3143..3757
                     /locus_tag="VFW59_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX5364508.1"
                     /translation="MTDFDSSTPDSNPKDAEYLARLQDYYADWKSIPSYASLCEVFGI
                     ASKSWVKAILDRLSKAGYLERTPDGMWIPTKQFFARPLAESSVPAGMPITVSATQGEH
                     FVIDEMLIDTPSKTTMIPVKGDSMIDAGIHDGDVAVVEKRLTANVGDIVVAIVDNEFT
                     LKTLDKERGQYILRPSNPAYPVIRPQGSLEIFGVLIGLIRKYRR"
     gene            3762..4232
                     /gene="umuD"
                     /locus_tag="VFW59_09575"
     CDS             3762..4232
                     /gene="umuD"
                     /locus_tag="VFW59_09575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEX5364509.1"
                     /translation="MQQVALSFGRRVASSALLSLTHPSKELFQRHRALLRKAVPAGFP
                     SCADDSIESHISLDEQLIQHRDSTFFMRVTGDSMRGLSIFDGDLLVVDRSVPATHGCV
                     VIAVVDGEFTVKQLMHTASGEVLRAAHPDYPDMVIRPEHDFSVWGVVQWNVHRM"
     gene            complement(4283..4371)
                     /locus_tag="VFW59_09580"
     tRNA            complement(4283..4371)
                     /locus_tag="VFW59_09580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4335..4337),aa:Leu,seq:taa)
     gene            complement(4379..4452)
                     /locus_tag="VFW59_09585"
     tRNA            complement(4379..4452)
                     /locus_tag="VFW59_09585"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4418..4420),aa:Cys,seq:gca)
     gene            complement(4507..4582)
                     /locus_tag="VFW59_09590"
     tRNA            complement(4507..4582)
                     /locus_tag="VFW59_09590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4547..4549),aa:Gly,seq:gcc)
     gene            complement(4643..5419)
                     /locus_tag="VFW59_09595"
     CDS             complement(4643..5419)
                     /locus_tag="VFW59_09595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5364510.1"
                     /translation="MPFIDSHCHINFPELVANLDEVLLQMQRNEVTAALCVAVNLADF
                     SQVLGLAERHPNIYASVGVHPDYEEVEEPSVARLVELAQHPRVVAIGETGLDYVRLKG
                     DLEWQRTRFRNHIRAARESAKPLIIHTREAAADTLRLMQEERAMEAGGVMHCFTENWE
                     VAEAALDMGFYISFSGIVTFKNAKQIKEVAQRVPLDRMLIETDAPYLAPVPHRGKLNQ
                     PAYVKHVAEEIATLRGITLAEVGQRTSENFFRLFGTDKKI"
     gene            complement(5422..>5802)
                     /locus_tag="VFW59_09600"
     CDS             complement(5422..>5802)
                     /locus_tag="VFW59_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX5364511.1"
                     /translation="SPLQAVKLDEQVGSEERGKLLRAIRQPETLDVFALAEALQKTEQ
                     VLVVQWLQQWCYDLSAMKLAGRLRYHLGEEAAIGKLVASVEPLNLARLQKYLQGAKRE
                     AQHTLNPKLFFESLLFSYCQLMLE"
BASE COUNT         1185 a         1768 c         1699 g         1150 t
ORIGIN      
        1 aaagaagcat gcaggaatac tggaaaagtt gggattattt agcccggaac aggaggaatt
       61 taacctccgc caagcgcgcc gcatgataac ccagccccat ggattcgcca atagaggctg
      121 gcaatgcgaa aactcaatgg ctcgccctca tccatgagcg attcgaaatg acagctaaaa
      181 gcttatcccc caacaaaaag ccaccccgat cggtgcgacg gggtggcttt tgtgaaaccc
      241 gaccgagctt tgctagcgcc ccagcacctt gcggtagacc tccagataag ccgccgcgct
      301 atgctcccag ctgaagtctt tggccatgca gttcttgcgc agggccttcc acttcttctg
      361 gtcgcgatag agttcgaccg cgcgctggat ggtggcatag aggttgtcgg cggtcatgcc
      421 gctgaacacg aagccgctgg cgctgccgtc cttgagcgtc gcatcggtgc agtcggtcac
      481 cgaatcggcc agaccgcctg tggcgtggac gatgggaggc gtgccgtagc gctggctgta
      541 gagctggttc aggccgcagg gttcgaagcg cgagggcatc acgaacatgt ccgagcccgc
      601 ttcgatcagg tgcgataact cttcgctgaa accgacatgg gcggcgatct tgcccggata
      661 gcgtgcggcc agctcgcgag ccgcgtcctg catctgcggc tcgccggaac cgagcaatgc
      721 gagttgcacc ggcagcccga ccagacgcgg ggcgatctcc agcagcaggt ccagaccctt
      781 ctggtaggtg aatcgactga ccacgccgag cagcggcacg tccgcctcga cggccagccc
      841 catgcgttcc tgcaacgcct gcttgttggc ggccttgccg gtcatgcgtg cggcgctgaa
      901 ttttttggcc agataaccat ccgtggccgg attccattcg tcggtgtcga tgccgttgag
      961 gatgccggtc aggctgtcgc ggcgttgcgc cagcagcccc tgcatgccga agcccagcgg
     1021 ctcctgctgg atctcctgcg cgtaattcgg gctgaccgtg gtgatatggt cggcataaaa
     1081 cagtccggcc ttgaggaacg acaggttgcc gtaatactcc accccgccga tagcgaagct
     1141 ggatggcggc agatgcaatt cgcccagcat ttgcggcgga aacacgccct gaaatgccat
     1201 attgtggatc gtcatcacgc tcggcacccc gccgtgccag agatgcaggt aggccggggc
     1261 gaggccggtc tgccagtcgt tgccatgcag cagatcgggc ttccatgcca gcggcgtatc
     1321 gtcgctggcg aggatcgccg cgaccctgga tagcagagcg aaacgccgcg cattgtccgt
     1381 ccagtcctgg ccgctggcat cctgataagg gccgcctgca cgctggtaca gctcgggaca
     1441 atccagcacc agcagcggca cgccgttctc ggtatcgccg gaaatcaggc gcgcataagg
     1501 gaagccgggc agatgctcaa aggtcgcgat cacttcgtgt tgcgacagtt gtgccagtac
     1561 cggtgtatag cccggcagca gcacgcgcac atccacaccg agcgcgcgca aggcggcggg
     1621 cagcgcgccg ctgacatcgg ccagtccgcc ggtcttgatc aggggtgcga gttcggaagt
     1681 cgcaaacagg actttcagtt cagacatgtt tcagttctcc atcacggccg gttcatctgg
     1741 ccgaagctgc gggattatag cgcagcgcag ttatcgactt gcgctcggtg ccgagcggcg
     1801 tatccgccca acggaattac gggcagaatt catgcggact gcgccgcacg caaggccgcg
     1861 cccagcaagg ccgtgcgatc gttcaggatc acccgcaccg gcatggcttc gagcaaggga
     1921 cgcatgcgcc ccttattgag gaaggcttcc aggaagacac cgtcctgtaa ctgcggcaat
     1981 attttcggcg cgatgccgcc ccccagatac aggcctccgc gactcatcac cttgagcgcc
     2041 agattgccgg cctccgcgcc atacaggcgc acgaagtgct gcagcacctc gacgcagagt
     2101 tcatcttttc cggcctgcgc cgcattggca atcgccgcag cggcatcgcc gttagtcatc
     2161 tcatccatca gccagtccgg cactgccgtg cggcgataca gacacaggaa ctggtacagg
     2221 ctcaatagcc ccatgccgga taccacacgc tcccagctga cgtggccgaa ctgttgtttg
     2281 aggtagagaa agaacgtcat ctccagctcg tcgctggggc tgaaactggc atgaccgcct
     2341 tcggtggcga aaggctggtg acgctgcccg tcccaataca gaccggcctc gcccagacct
     2401 gtgcccgccg cgatcacggc ggtattgccg ctggcgtcgg caacaccggc ttgcaggatc
     2461 agcaagtcag agtcatccaa tgcggggatg ccgaaggcgg tcgcctccag atcgttgagc
     2521 agcacgcagc cgaaaccgaa ttgccgttgc agcgcgtcgg cgtcaatacg ccagggcaga
     2581 ttggtggtct gcacgcccct gccctgcacc ggtccggcga taccgaaagc cgcacgggaa
     2641 ggcgcatcgg tcgcagccag aaattcgccc agcagcccct cgaaggtggc gtaatcgcgg
     2701 ctggcataac ggctctcatg gacgatcttt acgcgtgtac cgacgacctc gaccagcgca
     2761 agccttgtct tggtgccacc gatgtctcct gccagataaa tgtttttcat atcagaaagc
     2821 ccgtatatcg gcaccttcca gactcaaaga agggcgccag aattgattgc cccgacaaat
     2881 cgacaggcat tggatcgtat ccagacagca cagggctcgg atcaatttgc cgcgagcagc
     2941 aaagacagaa attcatgtcg cgcatgattg ccgagaccgt aacagtgttg attttagcat
     3001 gccgacggga gcggctctcg ccgctggcaa gcacggtgac gtcctatgta catggcagtg
     3061 ccaacattat tccccattcg attgacaaca gaacgtccgt tctttaatat gcgcagcacg
     3121 ttcgttctgt atggatatga ccatgactga cttcgatagc tccacacccg attccaaccc
     3181 caaggatgcc gagtatctcg ccagactgca ggactattac gccgactgga agagcatccc
     3241 ctcctacgct tcgctgtgcg aggtattcgg catcgcctcc aagtcatggg tcaaagccat
     3301 tctggaccga ctgagcaagg cgggctatct cgaacgcacg ccggatggca tgtggatacc
     3361 cacgaaacag ttcttcgccc gtccgctggc ggaatcatca gttccggcag gcatgccgat
     3421 cacggtcagc gccacccagg gcgaacattt tgtgatcgac gagatgctga tcgacacccc
     3481 gtccaagacc accatgattc cagtcaaggg cgactcgatg atcgatgcgg gcatacatga
     3541 cggcgatgtc gcggtggtgg aaaagcggct gacagccaac gtcggcgaca tcgtggtggc
     3601 catcgtggac aacgagttca ccctgaagac gctggacaag gagcgcgggc aatacatcct
     3661 gcgcccgtcc aatcccgcct atccggtgat ccgtccgcag ggttcgctgg aaatcttcgg
     3721 cgtgctgatc ggcctgatcc gaaaataccg gaggtaatgc catgcaacaa gtcgctctca
     3781 gttttggccg ccgtgttgcg tcctcagccc tgctctcgct gacccacccc agcaaagagc
     3841 tgttccagcg gcatcgcgcc ctgctgcgca aggccgttcc ggcaggcttt ccttcttgcg
     3901 ccgacgatag catcgaaagc catatcagcc tggacgagca attgatccag catcgtgact
     3961 ccaccttttt catgcgggtg acgggagact cgatgcgcgg cctgagtatt ttcgatggcg
     4021 acctgctggt cgtggatcgc tcggttcccg ccacccacgg ttgcgtcgtg attgcggtgg
     4081 ttgacgggga gttcaccgtc aaacaattga tgcataccgc gtcgggcgaa gtgctgcgtg
     4141 ccgcacaccc cgactatccc gatatggtca tcaggccgga gcatgacttt tccgtctggg
     4201 gcgtggtgca gtggaacgta cacaggatgt gatgacagct tcgtggctga tatttgaaac
     4261 ttgaaggggg cttaagcttt gatggtgccc aggaccggaa tcgaaccggt acggagattt
     4321 ctccccgacg gattttaagt ccgttgtgtc taccaatttc accacccggg cgggtatttg
     4381 gaggcgcgac ccagagtcga actgggctaa ccggatttgc aatccggggc ataaccgctt
     4441 tgctatcgcg ccgtgcgact gagctttaaa tgattaaggg aaagctaact tgctttccct
     4501 tgtgtttgga gcgggagaag aggctcgaac tctcgacctc aaccttggca aggttgcgct
     4561 ctaccaactg agctactccc gctgaacgag ttgcgcatta tagagagatt ttaattgctg
     4621 tcaacattcc gaactggaaa gtttatattt ttttatcggt accgaacaaa cggaaaaaat
     4681 tctcgctggt gcgctgcccg acttcggcga gcgtgatgcc gcgcagcgtc gcgatctctt
     4741 ccgcgacatg cttcacataa gccggttgat tgagtttgcc gcgatgaggc acgggggcga
     4801 gataaggcgc atcggtctcg atcagcatgc gatccagcgg cacgcgctgt gcgacctcct
     4861 tgatctgttt tgcattcttg aaggtgacga tgccggaaaa ggaaatgtag aaacccatgt
     4921 ccaatgcggc ctcggccact tcccagtttt cggtgaagca atgcatcacg ccgcccgcct
     4981 ccatcgcccg ctcttcctgc atcagacgca gggtatcggc tgccgcctcg cgggtatgaa
     5041 tgatcagcgg cttggcgctt tcgcgtgccg cgcggatatg gttgcgaaag cgcgtgcgct
     5101 gccactccag atcgcctttc agcctgacgt aatccagccc ggtctcgccg atggcgacga
     5161 ctctggggtg ctgcgccagc tcgaccaaac gggcgacaga gggctcctcc acctcttcgt
     5221 aatcgggatg cacgccgacc gaggcgtaga tgttcggatg ccgttcggcg agccccagca
     5281 cctgcgaaaa atcggccaga ttgacggcga cgcacaaggc cgccgtcacc tcgttgcgct
     5341 gcatctgcag caacacctcg tcgaggttgg cgaccagttc gggaaaattg atgtgacagt
     5401 gcgaatcgat aaacggcatg gttattcaag catcagctgg cagtaggaga acaacaggga
     5461 ctcgaagaac agcttgggat tgagcgtgtg ctgcgcctcg cgcttcgcgc cttgcagata
     5521 tttttgcagc cgcgccagat tcagcggctc gaccgatgcg accagcttgc cgatcgcggc
     5581 ttcctcgccg aggtgataac gcaacctgcc agccagcttc atcgcactca ggtcgtagca
     5641 ccattgttgc agccactgca cgaccagcac ctgttcggtc ttttgcagcg cctcggccag
     5701 cgcgaacacg tccagcgtct cgggctggcg tatggcgcgc aacagcttgc cgcgctcctc
     5761 gctgcccacc tgttcatcca gtttaaccgc ctgcaatggc ga
//
LOCUS       DASUIV010000265         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_162673, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000265 DASUIV010000000
VERSION     DASUIV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162673"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /gene="cobT"
                     /locus_tag="VFW59_09605"
     CDS             complement(<1..1007)
                     /gene="cobT"
                     /locus_tag="VFW59_09605"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX5364512.1"
                     /translation="MMNTNWLAQRCATLNEIARSAALARQGQLTKPPGSLGLLEDIAV
                     RLAAMQGTERPAADKAAIVIYAADHGIAAENVSAFPQAVTGEMVKNFARGGAAISVLA
                     KTLDARLEVVNLGTVNDPGDWPNVVRAVIAPSTANFAAGPAMTEEQLEQALQAGRDSV
                     LRAQQQGAQLFIGGEMGIANTTAATALACALLGATPTELAGPGTGLDHAGVAHKAEVI
                     RRALELHGFLPADHEKWIGENVLKVLRHIGGFEIAALTGAYLTCAQQGLPVLVDGFIS
                     SVAALVAVRFNPEVGDWLLFAHVSAEPGHQRVLEELEAQPLLSLGMRLGEGSGAASAL
                     PL"
     gene            complement(1004..1522)
                     /gene="cobU"
                     /locus_tag="VFW59_09610"
     CDS             complement(1004..1522)
                     /gene="cobU"
                     /locus_tag="VFW59_09610"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX5364513.1"
                     /translation="MKELILGGVRSGKSRLAEQHARESGLDVLYVATAQVRDEEMQRR
                     VAEHRVRRPGSWRSVEAGQNLAEVLQREAQAGRCVLVDCLTLWLTQLLCDADEAVLRS
                     EVDALLEALPVLPGHIILVSNETNMGVVPMGELSRRFCDEAGRLHQQIAASCDRVILT
                     VAGLPLVVKGNK"
     gene            complement(1519..3024)
                     /locus_tag="VFW59_09615"
     CDS             complement(1519..3024)
                     /locus_tag="VFW59_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX5364514.1"
                     /translation="MNARTVMVQGTTSDAGKSTLVAALCRWLVRQNVSVVPFKPQNMA
                     LNSAVTSDGGEIGRAQAVQAQAARLVPHTDMNPVLLKPNSDTGAQVIIHGRAIGNMDA
                     LEYHAYKHRARTAVLESHDRLSEQYELVVVEGAGSPAEINLRANDIANMGFAEAVDCP
                     VILIADIDRGGVFAHLVGTLALLSESEQARVAGFVINRFRGDLSLLQPGLDWLEQHTG
                     KPVIGVLPYLHGLHLEAEDAVPASLPSPASGGGVGGEGGLRVVVPVLPHISNHTDFDP
                     LRLHPQVEFRYVAMGEAIPSADLIVLPGSKSVRSDLDSLRRAGWDAAIDRHLRYGGKV
                     IGICGGLQMLGRSIHDPFGVEGEPGSSQGLGLLAFDTTLETEKQLRNVSGTLSLEDAP
                     VAGYEIHAGVSSGEALAHPALRLEQGADGAMSPDGAMSGDGQILATYLHGLFESRQAT
                     DALLRWAGLSNASTPDYQARCEADIDRLADAVEQYLDGKQLRALFGLGEKQ"
     gene            complement(3021..>3311)
                     /locus_tag="VFW59_09620"
     CDS             complement(3021..>3311)
                     /locus_tag="VFW59_09620"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="cobalamin biosynthesis protein; decarboxylates
                     L-threonine-O-3-phosphate to yield (R)-1-amino-2-propanol
                     O-2-phosphate, the precursor for the linkage between the
                     nucleotide loop and the corrin ring in cobalamin;
                     structurally similar to histidinol phosphate
                     aminotransferase; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase"
                     /protein_id="HEX5364515.1"
                     /translation="MLALSDRDWQGTTRESLPSASQRLARLLDTYGLPPTGGCSLFQW
                     VRRIDAASVHEQLAQQGILTRFFAEPASLRFGLPRDEAEWTRLSDALNRVTR"
BASE COUNT          552 a         1190 c          983 g          586 t
ORIGIN      
        1 agcggcaagg cgctcgccgc gccgctgccc tcgcccagac gcatgcccag actcagcaac
       61 ggttgcgctt cgagttcctc gagtacgcgc tggtgtcccg gctcggcaga tacatgcgcg
      121 aacagcagcc agtcgcccac ctccggattg aaacgcaccg caaccagcgc ggcgaccgag
      181 ctgatgaaac catccaccag caccggcagg ccttgttgcg cgcaggtgag ataggcgccg
      241 gtcaacgcgg cgatctcgaa gccgccgata tggcgcagca ccttgagcac attttcgccg
      301 atccattttt catggtcggc gggaaggaag ccgtgcaact ccagcgcgcg ccggatcacc
      361 tcggccttgt gcgccacccc cgcatgatcc aggccggttc ccggccccgc cagttctgtc
      421 ggcgttgcac cgagcagcgc gcaggccagc gccgtggcgg cggtggtgtt ggcgatgccc
      481 atctctccgc cgatgaatag ctgcgcaccc tgctgctgcg ccctcagcac gctgtctcgc
      541 cccgcctgca gcgcctgttc cagctgctcc tcggtcatcg cgggcccggc cgcaaaattc
      601 gccgtcgatg gcgcgatcac cgcgcgcacc acattcggcc agtcgcccgg atcgttgacc
      661 gtgccgaggt tcaccacttc cagtctggcg tccagtgtct ttgccagcac gctgatcgcc
      721 gcaccgccgc gcgcgaaatt cttcaccatc tcgccggtca ccgcctgcgg gaatgcagag
      781 acgttctcgg ccgcgatgcc gtgatcggcg gcgtagatca cgatggcggc cttatccgct
      841 gcggggcgtt ccgttccctg catcgcggcc agccgcaccg cgatgtcttc cagcagcccc
      901 agagagccgg gcggcttggt gagctgcccc tgtcgcgcca gagcggcgct gcgggcgatt
      961 tcgttcaagg ttgcgcagcg ttgtgccagc caattcgtat tcatcatttg tttcctttca
     1021 cgaccagcgg caatcctgcc acggtcaaaa tcacgcgatc gcatgacgca gcgatctgct
     1081 gatgcaagcg ccccgcttcg tcgcaaaacc ggcgcgacaa ttctcccatc ggcacgacgc
     1141 ccatattggt ctcgttgctc accagaataa tgtgtccggg caggacaggt aacgcttcca
     1201 gcaaggcgtc cacttcgctg cgcagcactg cttcgtccgc atcgcacagc aactgcgtca
     1261 gccacagcgt caggcagtcc accagcacgc atcgccctgc ctgcgcctcg cgttgcaaca
     1321 cctcggccag attctgaccg gcctcgaccg agcgccagct gcccggcctg cgcacccgat
     1381 gctccgcgac tcgccgctgc atctcctcgt cgcgcacctg cgcggtggcg acatacagaa
     1441 cgtccagccc cgactcacgc gcatgctgct ccgccaagcg gctcttgccg gaacgcacgc
     1501 cgcccagtat cagttctttc attgtttttc tcccaatccg aacagggcgc gcaactgctt
     1561 gccatcgaga tactgctcca ccgcatcggc cagccggtcg atgtcggcct cgcagcgcgc
     1621 ctgatagtcg ggcgtgctcg cgttcgacaa tccggcccag cgcagcagcg catcggtcgc
     1681 ctgacgcgac tcgaacaggc cgtgcagata ggtcgcgagt atctgtccgt cgccggacat
     1741 cgcaccatca ggcgacatcg cgccatccgc cccctgttcg agtcgcagcg ccgggtgtgc
     1801 cagcgcctcg ccgctcgaca ctccggcatg gatttcgtag cctgcgaccg gtgcatcttc
     1861 cagcgacagc gtaccgctga cattgcgcag ctgcttttcg gtttccagcg tggtgtcgaa
     1921 tgccagcagc cccaagcctt ggctgctgcc gggctctccc tccacgccga acgggtcatg
     1981 gatggatcgc cccagcatct gcaggccgcc gcagatgccg atgaccttgc cgccgtagcg
     2041 caaatgcctg tcgatagccg catcccatcc ggcacggcgc agactatcca gatcgctgcg
     2101 caccgacttg ctgccgggca gcacgatcag gtcggcagag ggaatcgcct cgcccatcgc
     2161 gacataacgg aactcgacct gaggatgcag gcgcagcgga tcgaagtcgg tgtggttgct
     2221 gatgtgggga aggacgggaa cgacgacgcg caatccaccc tctcccccaa cccctccccc
     2281 gctcgcgggg gaggggagcg atgcgggcac cgcatcctcg gcctccagat gcaggccgtg
     2341 cagatagggc aacacgccga tcaccggctt tccggtgtgc tgctccagcc agtccagacc
     2401 gggctgcaac agcgacaggt cgccgcgaaa gcggttgatc acgaaacccg ccacgcgcgc
     2461 ctgctcgctt tcgctcagca gcgccagcgt gccgaccaga tgcgcgaaca cgccgccgcg
     2521 atcgatgtcg gcgatcagga tcaccgggca atcgaccgcc tcggcgaagc ccatgttggc
     2581 gatgtcgttc gcgcgcagat tgatctcggc agggctgccc gccccctcga ccacgaccag
     2641 ctcgtactgc tcgctcaggc gatcatgcga ctccagcacc gcagtgcgcg ccctgtgctt
     2701 gtatgcgtgg tattccagcg cgtccatgtt gccgatcgcg cggccgtgga tgatgacctg
     2761 cgcgccggtg tcgctgttcg gtttgagcag caccggattc atgtcggtgt gcggcacgag
     2821 gcgcgcggcc tgtgcctgca ccgcctgcgc gcgcccgatc tcgccgccat cggaagtgac
     2881 cgcgctgttc agcgccatgt tctgcggctt gaacggcacg acggaaacat tctgccgaac
     2941 cagccagcgg cacagtgcgg cgaccagcgt gctcttgccc gcgtccgagg tcgtgccctg
     3001 caccatcacg gttctggcgt tcatcgcgtc accctgttca atgcgtcgga aagtcgagtc
     3061 cattccgcct cgtcgcgcgg caggccgaaa cgcaggctcg cgggctcggc aaaaaaacgt
     3121 gtgagtatcc cttgttgcgc gagctgctca tgtacgctcg ccgcatcaat gcgacgcacc
     3181 cactggaaca ggctgcaccc tcctgttggc ggcaaaccat acgtatccag caaccgggcg
     3241 aggcgttgcg atgccgacgg cagggattct cttgtcgtac cttgccagtc cctgtcgctc
     3301 agcgcgagca t
//
LOCUS       DASUIV010000266        10271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_349647, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000266 DASUIV010000000
VERSION     DASUIV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349647"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="VFW59_09625"
     CDS             <1..1562
                     /locus_tag="VFW59_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5364516.1"
                     /translation="PSLLPAVKAAASGFALLLGFAFLPLLQLRRVSPLRVLRRELGAP
                     QPNTLLAYGLALLVLAGLFLWHAGSLKLGLAVLGGLLAGLVVFGALAWLMLHGLARYA
                     FLFGTHARQAANNLLRHGRSNALQIVALALGGMALLLLTLVRADLLQGWEGKLPPDTP
                     NRFLVNIQPDQQQPVLDFFASQQLPQPQLLPMVRGRLVEINEHPVNGDSYADTRASHL
                     VEREFNLSYMERMPEWNELVGGAWWQREGDAAGIGLTERPASLRADAASVPLGNPPAS
                     PLRGQKVDRGQLSVEEGIANTLGIHLGDTLTYDVAGSRFTALVTSLRKVQWDTMRVNF
                     FVIGTPDLLRDFPASYLTSFYLPPDKVRVGDELARAFPNLLVIDTGEIIDQVRHIMDQ
                     ITQVMGAVFLFTLLSGFAVLYAALLATQDERSFEAAVLRTLGADSRYLRKLHLAEFAV
                     LGGLSGLFASAGAVLLGWVLARFVLEIPYQPSLAIWPIGVLGGMAAVMLAGRLGTRRL
                     VTLPPLAILRD"
     gene            complement(1559..2632)
                     /locus_tag="VFW59_09630"
     CDS             complement(1559..2632)
                     /locus_tag="VFW59_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364517.1"
                     /translation="MKIENSTVGMSSQHASSRQVTVEETLRVWKGAQRPDFEGRGINA
                     IAQTMVDLSEAGRQAAAQTAANHPAANAASGTEDATSPAVNDPKMLMLIGLIESLTGR
                     KVRLFDPGMLKLGEVTDAHIQQEQAKAQSPQRQGWGVEYDRHETLRETEQTRFEAQGV
                     VRTSDGREIRFQLSLEMKREFFQETNLSVRAGDGVRKDPLVINFAGTAAQLTDQKFAF
                     DLNSDGQTEHVSFVGTGSGFLALDKNGNGTIDNGSELFGTQSGNGFADLSAYDSDKNG
                     WIDENDAVYSKLRVWSKDAGSHDQLSTLAQRNVGALYLGNVATPFDLKNGANALQGQV
                     RSSGVYLNEDGGAGTLQQVDLVV"
     gene            2826..3686
                     /locus_tag="VFW59_09635"
     CDS             2826..3686
                     /locus_tag="VFW59_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX5364518.1"
                     /translation="MHIPGSMLQNTLCTVTLAAAAASLVAAVHFARKAKDRPTSLGFG
                     ALTALIFAAQMMNFPVMNGVSGHLLGGVLAAALLGIPFGVLSVALVISVQALLLGDGG
                     LDALGANIFNMAVLGAGVGGVLYQALHARCSKPLAMAIASWGAVMLAALAVSLELGGT
                     VAFLAVAPSMLGVHALVGIGEALIAVLTFELLSSTAVTGGRHASGWVSSGLAAMLVLF
                     SPFASALPDGLAWVVAQQGLAPAQAIAPLIHMGIPLEMTSMVGALVSFAAGWGMLRIM
                     NRPLGKRMGS"
     gene            3876..4598
                     /gene="rpsB"
                     /locus_tag="VFW59_09640"
     CDS             3876..4598
                     /gene="rpsB"
                     /locus_tag="VFW59_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5364519.1"
                     /translation="MAVTMRQMLEAGVHFGHQTRFWNPKMAPYIFGHRNKIHIINLEK
                     TVMKFAEAEAFVRKLAAKKGTILFVATKRQARDIIQEEAVRADSPFVNHRWLGGMLTN
                     HKTVQLSIKRLRELEELTADGASEKISKKEALMMKRELEKLDRSLGGIKDMQGLPAAL
                     FVIDVGYQKGAIVEAKKLGIPVIGIVDTNNSPLDVDFVIPGNDDSTQAIRLYARGIAD
                     AVLEGRAQATAELVEQIAEQAA"
     gene            4664..5563
                     /gene="tsf"
                     /locus_tag="VFW59_09645"
     CDS             4664..5563
                     /gene="tsf"
                     /locus_tag="VFW59_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5364520.1"
                     /translation="MAEVTASMVKELREATGLGMMECKKALVEADGDLKVAEEQLRIK
                     SGAKASKAASRVTAEGLVSAYLAQDAKTGAVVEVNCETDFVGKNDDFIAFAKNVAETV
                     AKNNPADLEALSGMALANDTGTVEEVRKALVMKIGENVSIRRFERYETADGQLSAYLH
                     GSKIGVLVNFTGGDEAMGRDIAMHIAASKPKSVDASGVNPEDIATERRIAIEKAKESG
                     KPEAMLEKIAEGTVQKFLKEVTLLGQVFVKAEDGKQTIEQLLKSKNASVVAFQMFVVG
                     EGIEKKVEDYAAEVAAAAAAAKG"
     gene            5569..6285
                     /gene="pyrH"
                     /locus_tag="VFW59_09650"
     CDS             5569..6285
                     /gene="pyrH"
                     /locus_tag="VFW59_09650"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5364521.1"
                     /translation="MKPVYKRILLKLSGEALMGEDSYGINREVIERIVGEIKEVVELG
                     VEVAIVIGGGNIFRGVAPAAAGMDRATADYMGMLATVMNSMALQDAMKRAGLDCRVQS
                     ALSLEQVAEPFIRGKALRYLEDGKVVIFAAGIGSPFFTTDTAAALRGVEMSADVVIKA
                     TKVDGIYTADPKKDPNAIRYQSLSFDEAISKNLKVMDATALTLCRDQKLPIIVFSIFK
                     TGALKRVIFGEGEGTLVRID"
     gene            6336..6857
                     /gene="frr"
                     /locus_tag="VFW59_09655"
     CDS             6336..6857
                     /gene="frr"
                     /locus_tag="VFW59_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5364522.1"
                     /translation="MQKSLESLRADLGKVRTGRAHIGLLDHVMVDYYGNPTQVNQVAN
                     ITLVDARTIGVQPFEKNMVGAIERAIRDSDLGLNPSTNGELIRVPMPMLTEERRRDMI
                     KIIKSEGEDAKIAVRNIRRDANHTLKEALKKKEIAEDEERRTQDDIQKLTDRFVAEID
                     KALAAKEVDLMAV"
     gene            6937..7713
                     /locus_tag="VFW59_09660"
     CDS             6937..7713
                     /locus_tag="VFW59_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX5364523.1"
                     /translation="MAIFQSSTSTIPDIPSVPRHIAVIMDGNGRWAKKRFMPRVMGHQ
                     RGVESLREVVRACRDLGVEYLTVFAFSSENWRRPADEVSFLMSLFLKMLEREIASMHK
                     NNIRLKIIGERADFDDKLRQTMQEAEQLTANNTGLTLTIAANYGGRWDMMHAVQAMLK
                     DHPQLAQGFTEEDLSPYLSMSDAPEPDLFIRTGGEQRISNFMLWQLAYTELYFTDVLW
                     PAFGRRELEAAIASYQKRERRFGRTSEQLTGEPIKGENGA"
     gene            7706..8536
                     /locus_tag="VFW59_09665"
     CDS             7706..8536
                     /locus_tag="VFW59_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5364524.1"
                     /translation="MLKLRVITAIVLVVVLLAALFLLPPLGWALLVVLMVMQGTSEWS
                     RLAGLSGARANLYWGLTLVLMLGLLWLDADATETARRYVHLAIYTISAFLWLIVVPSW
                     LIVGWKVKNPVWMALTGWAVLIPTGVAMMDLREAAPGPWVLLFVMGLVWAADIAAYFA
                     GRRFGKNKLAPSISPGKTWEGVGGAMVGVSVYVVLVWWLSPYFATRGILPPLLLASWW
                     WVALAVIGDLFESAIKRQAGVKDSGALLPGHGGLLDRIDALTSTLPLAAFVLLSQVVA
                     "
     gene            8540..9745
                     /gene="ispC"
                     /locus_tag="VFW59_09670"
     CDS             8540..9745
                     /gene="ispC"
                     /locus_tag="VFW59_09670"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX5364525.1"
                     /translation="MQNLTVLGSTGSIGTSTLDVVARHPERYRVVALTANSQVDLLFR
                     QCEQFKPDYAVLLDEASAAGLRKRLNDAGLVTEVLCGVEALEQVSVLPEVDAVMAAIV
                     GAAGLRPTLAAARAGKKILLANKETLVMAGNVFMEAVRTSGSALLPIDSEHNAIFQSL
                     PRGYDGDMARNGVSRILLTASGGPFRNTPLAELRNVTPEQACAHPNWSMGRKISVDSA
                     SMMNKGLEVIEAHWLFNASADDIQVVVHPQSVIHSLVQYVDGSVMAQLGNPDMRTPIA
                     YGLAYPERIDAGVAPLDLFKIATMNFVAPDFERFPCLALAYQALRTAGTAPAILNAAN
                     EVAVDAFLNRRIAFLDIPRVIATVLDALPVNAVSCLDDVVDADAEARRAALQQIGNLA
                     ARPAVPLHP"
     gene            9742..>10271
                     /gene="rseP"
                     /locus_tag="VFW59_09675"
     CDS             9742..>10271
                     /gene="rseP"
                     /locus_tag="VFW59_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX5364526.1"
                     /translation="MITLLAFIAAIALLVVFHELGHYWVARRCGVKVLRFSLGFGRVM
                     YSKRCASGTEWVISALPLGGYVKMLDEREEAVSPEELDQAFNRKPVLQRMAIVAAGPL
                     ANFLLAILLYWVMFIHGVPGLKPVLGEVPSGTPAAVAQMQSGETILSVDGMLIPKWQE
                     LRWTLLDLALQQSEVR"
BASE COUNT         1874 a         2837 c         3398 g         2162 t
ORIGIN      
        1 agccatcgct gctgcccgcg gtcaaggccg ccgccagcgg cttcgcgctg ctgctgggct
       61 tcgccttcct gccgttgctg caactgcgcc gcgtgtcgcc cttgcgcgtg ttgcggcgcg
      121 aactcggtgc gccgcagccg aacaccctgc tggcttacgg actggcgctg ctggtgctgg
      181 cgggattgtt cctgtggcat gcgggttcgc tgaaactggg gttggcggtg ctgggcggcc
      241 tgctcgccgg gttggtggta ttcggtgcgc tggcctggct gatgctgcat ggtctggcgc
      301 gctatgcctt cctgtttggc actcatgcca gacaagcggc aaacaatctg ctgcggcacg
      361 ggcgcagcaa tgccctgcag atcgtcgcgc tggcgctggg cggtatggcc ttgctgctgc
      421 tgacgctggt gcgcgccgat ctgctgcaag gctgggaggg caagctgccg ccggatacgc
      481 cgaaccgttt tctggtgaac atccagcccg accagcaaca gccggtgctg gatttcttcg
      541 cgagccagca gttgccgcag ccgcagttgc tgccgatggt gcgcggacgg ctggtcgaga
      601 tcaacgagca tccggtgaac ggcgacagct atgccgatac gcgcgcgagc catctggtcg
      661 agcgcgagtt caatctctcc tatatggagc gcatgccgga atggaacgaa ctggtcggag
      721 gtgcatggtg gcagcgggag ggtgatgccg caggcatcgg gctgactgag cggcctgcaa
      781 gcttgcgagc agatgccgcc agcgtccctt tggggaaccc accggcgtcc cccttgcggg
      841 gacagaaggt tgacaggggg caactgtcgg tcgaggaggg catcgcgaat acgctgggca
      901 tccatctcgg cgacactctg acctacgatg tcgcaggcag tcgtttcacc gcgcttgtga
      961 ccagcttgcg taaagtacag tgggacacga tgcgggtgaa tttcttcgtg atcggcacgc
     1021 ccgatctgtt gcgcgacttt ccggcaagct atctcaccag cttctacctg ccgccggaca
     1081 aggtgcgcgt cggcgacgaa ctggcgcgcg cgttccccaa cctgctggtg atcgataccg
     1141 gcgagatcat cgatcaggtg cgccacatca tggatcagat cactcaggtg atgggtgcgg
     1201 tgttcctgtt cacattgctg tccggcttcg cggtgctgta tgccgcgctg ctcgcgaccc
     1261 aggacgaacg cagtttcgag gcggcggtgc tgcgcacatt gggcgcggac agccgctatc
     1321 tgcgcaagct gcatctggcc gagttcgccg tgctgggtgg tctgagcggc ctgttcgcgt
     1381 cagccggggc ggtgctgctg ggctgggtgc tggcgcgctt cgtactggag ataccctacc
     1441 agccgagtct cgcgatctgg ccgatcggcg tgctgggcgg catggcggcg gtgatgctgg
     1501 cgggcaggct gggcaccaga aggctggtta cgctgccgcc gctggcgata ctgcgcgact
     1561 agaccaccag atcgacctgc tgcagcgtcc ccgcaccgcc gtcttcgttc agatacacgc
     1621 cgctgctgcg cacctgtccc tgcaacgcat tcgcgccgtt cttcaggtcg aacggggtgg
     1681 cgacgttgcc cagatacagc gcgccgacgt tgcgctgcgc cagcgtgctg agctggtcat
     1741 ggctgccggc atccttgctc catacccgca gtttcgaata tacggcgtcg ttctcgtcta
     1801 tccagccgtt cttgtcgctg tcgtaggcgg acaggtcggc aaagccgttg ccgctctggg
     1861 tgccgaacag ttccgagccg ttgtcgatgg tgccgttgcc gttcttgtcc agtgcgagga
     1921 agccgctgcc tgtgcctacg aaggagacat gttcggtctg gccgtcgctg ttcaggtcga
     1981 aggcgaactt ctggtcggtg agctgggcgg cggtgcctgc gaagttgatc accagcgggt
     2041 ctttcctgac gccatcgcct gcgcgtacgg agaggttggt ctcctgaaag aactcgcgct
     2101 tcatctccag cgagagctgg aagcggatct cgcggccgtc gctggttctg accacgccct
     2161 gtgcctcgaa acgggtttgc tccgtttcgc gcagggtctc gtggcggtcg tattccacgc
     2221 cccagccttg ccgttgcggg ctctgcgctt tggcctgttc ctgttgtatg tgcgcatcgg
     2281 tgacttcgcc cagcttcagc atgcccggat cgaacagcct gaccttgcgg cccgtcagtg
     2341 attcgatcag cccgatgagc atcagcatct tcggatcgtt gacggcggga ctggtcgcgt
     2401 cctcggtgcc ggaagccgcg tttgcggcgg gatggttcgc tgcggtttgc gcggcggcct
     2461 gacggccagc ctcggacagg tctaccatgg tctgagcgat agcattgatg ccgcgacctt
     2521 cgaagtcggg acgttgcgcg cctttccaga cgcgcagcgt ctcttcgacg gttacctgtc
     2581 gtgaactggc atgctggctg ctcatgccga cagtcgagtt ttcgattttc atcgcaatgc
     2641 cctccatgta gctatgactg tatatcgacg tgcagacgcg atgtattaga gttggcggtg
     2701 aaaatcaggt aaccctgact tgttgcggca gcggataagg cgtttaattt ccgaccgttc
     2761 aagcgatgga cggttcgttc aggcttattt cctattcctc acgctattcg agcaaggaga
     2821 acacgatgca tattcccggt tccatgctgc aaaacaccct ctgtaccgtc actctggccg
     2881 ctgctgcggc gagtctggtc gctgccgttc atttcgccag aaaagccaag gacaggccga
     2941 cctcgctcgg attcggcgcg cttaccgcgc tgatcttcgc ggcgcaaatg atgaactttc
     3001 ccgtcatgaa cggcgtatcc gggcacctgc tcggcggcgt gctggcggcg gccttgctgg
     3061 gtattccgtt cggcgtcctg tcggtcgcgc tggtgatttc ggttcaggcg ttgctgctgg
     3121 gcgatggcgg tctcgatgcg ctaggtgcca atattttcaa catggcggtg ctgggcgcgg
     3181 gtgtcggtgg tgtcttgtat caggcgttgc atgctcgttg ttcgaagccg cttgcgatgg
     3241 ccatcgcatc ctggggggcg gtcatgctgg cggcactcgc cgtgagtctg gagctgggcg
     3301 ggacagtcgc tttcctcgcc gtcgcgccgt caatgctggg tgttcatgcg ctggtcggca
     3361 tcggggaggc gctcatcgcg gtcttgacct tcgagttgct gtcgtcgact gcggtaacgg
     3421 gaggccggca cgcaagcggt tgggtgtcgt cgggtctggc ggcaatgctg gtgttgttca
     3481 gccccttcgc ttccgccttg ccggatgggt tggcgtgggt cgtagcgcag cagggattgg
     3541 cgccggcaca ggcaatcgca ccgttgatac atatggggat tccactggaa atgacgagca
     3601 tggtcggggc gctggtctcg tttgcggctg gctggggcat gttgcgcatc atgaaccggc
     3661 cccttggaaa gcgcatgggc agctgactcg ccgatttcga cctgtcttgc gccctgccgg
     3721 acaggttgag tggaacggtg cattggtgtt agaatgcgcg ccgctccgga ataggccgga
     3781 gtgaatttcc cgctcgcgca agttggggtg ctcacaggat cgctgtgggt caggctggcg
     3841 agtggtagac aataaccctt acgttaggag tagtcatggc tgtaactatg cgtcaaatgc
     3901 tggaagctgg tgtccatttc ggtcaccaaa cccgtttctg gaacccaaag atggcaccgt
     3961 acatcttcgg tcatcgcaac aagattcaca tcatcaacct ggaaaagact gtcatgaagt
     4021 ttgccgaagc ggaagctttc gtgcgcaagc tggcagccaa gaagggcact atcctgttcg
     4081 ttgcaaccaa gcgccaggct cgtgacatca tccaggaaga agccgtacgt gcagattccc
     4141 cgttcgttaa ccatcgctgg ttgggcggta tgctgaccaa ccacaagact gttcaactgt
     4201 ccatcaagcg tctgcgcgaa ctggaagagc tgactgccga tggtgcatcc gagaagattt
     4261 ccaagaaaga agcgctgatg atgaagcgcg agctggaaaa gctggatcgc agtctgggcg
     4321 gcatcaagga catgcaaggt ctgccagcag cgctgttcgt gatcgacgtc ggctaccaga
     4381 agggtgccat cgtcgaggcg aagaagctgg gcattccagt gatcggcatc gtcgatacca
     4441 acaactctcc gttggatgtg gacttcgtga tcccgggtaa cgacgattcg acacaggcga
     4501 tccgtctgta cgctcgcggt atcgccgatg cggtgctgga aggtcgtgcg caagcaacgg
     4561 ccgagctggt cgagcagatc gcagaacaag cagcctaagt ctcaacaagg cgcataaagg
     4621 ggcgcgagcc ccttttttta taagttttta ttggagcaag aagatggcag aagttaccgc
     4681 aagcatggtt aaagagctgc gcgaggcgac cggcctcggc atgatggaat gcaaaaaggc
     4741 gctggttgaa gccgacggcg atctgaaagt ggctgaagaa cagctgcgta tcaagagtgg
     4801 cgcgaaggca agcaaggctg cttcccgcgt gaccgcagag ggtctggtca gcgcgtatct
     4861 cgcgcaagat gccaagaccg gtgcagtggt tgaagtgaac tgcgagaccg acttcgtcgg
     4921 caagaacgac gacttcatcg ctttcgcgaa gaatgtcgcc gagaccgtcg ccaagaacaa
     4981 cccggccgac ctcgaggcct tgtccggcat ggcgctggct aacgataccg gcaccgtgga
     5041 agaagtgcgc aaggcgctgg tgatgaagat cggcgagaac gtctccatcc gtcgtttcga
     5101 gcgttatgag actgctgatg gccagttgtc tgcctacctg catggcagca agatcggcgt
     5161 gctggtgaac tttaccggcg gcgacgaagc catgggccgc gacatcgcga tgcacattgc
     5221 agcttccaag ccgaagtcgg tcgatgcctc tggcgtaaac ccggaagata tcgcgaccga
     5281 gcgtcgtatt gcaatcgaga aggccaagga gtccggcaag ccggaagcga tgctggagaa
     5341 gatcgccgaa ggtacggtgc agaagttcct caaggaagtg acgctgctgg ggcaggtgtt
     5401 cgtcaaggct gaagacggca agcagaccat cgagcaattg ctgaagagca agaatgcttc
     5461 tgtcgttgcg ttccagatgt tcgtggtggg cgagggcatc gagaagaaag tggaagatta
     5521 cgcagctgaa gtcgctgctg ctgcagctgc tgcaaagggt taatcagcat gaagccagtc
     5581 tacaaacgca ttctgctcaa gctgtcgggc gaggccctga tgggcgaaga cagctacggc
     5641 atcaaccgtg aagtgatcga gcgtatcgtc ggcgagatca aagaggtggt tgagctgggt
     5701 gttgaggtag cgattgtgat cggtgggggc aacatcttcc gaggcgtggc tccggctgcg
     5761 gcagggatgg atcgcgcgac cgccgattac atgggaatgc tggcgacagt gatgaattcg
     5821 atggcactgc aggacgcgat gaagcgtgcc gggctggact gccgcgtgca gtccgcgttg
     5881 agcctggagc aggtggccga gccgtttatt cgcggcaagg cattgcgtta tctggaagac
     5941 ggcaaggtgg tgatcttcgc ggctggcatc ggcagcccgt tcttcaccac tgacaccgcg
     6001 gccgcgttgc gcggcgtgga aatgtctgcc gatgtggtga tcaaagccac caaggtcgac
     6061 ggtatctata ctgccgatcc gaagaaggac cccaatgcga tccgttatca gagcctgagc
     6121 tttgatgagg cgatcagcaa gaatctcaag gtgatggatg caactgcgct gacattgtgt
     6181 cgcgatcaga agctgcccat tatcgtattc agtattttta aaaccggtgc gctcaagcgt
     6241 gtgatattcg gtgaaggcga aggcacgctg gttcgtatcg attgattcag gagggaattg
     6301 tggttgctga tctgaaaaaa actgccgaac aaaagatgca gaagtcgctg gagtcattgc
     6361 gggcagactt gggcaaggtg cgcaccgggc gtgctcacat cggtctgctg gaccatgtga
     6421 tggtggatta ctacggtaac ccgacgcagg tgaatcaggt ggcgaacatt acgctggtcg
     6481 atgcccgcac gatcggcgtg cagcctttcg aaaagaacat ggtcggtgcg atcgagcgcg
     6541 cgatccgcga ctccgatctg gggctgaatc cttcgaccaa tggcgaactg atccgcgtgc
     6601 cgatgccgat gctgaccgaa gagcgtcgcc gcgacatgat caagatcatc aagagcgagg
     6661 gcgaggacgc caagatcgcg gtgcgcaata ttcgccgcga tgccaatcac acgctgaaag
     6721 aagcgcttaa aaagaaagag atcgctgagg acgaagagcg ccgcactcag gatgatatcc
     6781 agaaactgac tgaccgcttc gtcgctgaaa tcgacaaggc cctggccgcc aaagaagtag
     6841 acttgatggc ggtctgatag gactggggag cgaggatcgg ggatcgggta agctcgtttt
     6901 tcctcaaccc tcgatcctca atcctcgatt ctgaacatgg ccatcttcca gagctccacc
     6961 agcaccattc ccgacattcc cagtgtcccg cgccacattg ccgtcatcat ggacggcaat
     7021 gggcgttggg cgaaaaaacg tttcatgccc cgtgtgatgg gccatcagcg cggcgtcgaa
     7081 tcgttgcgcg aggtggtgcg tgcctgtcgc gatctggggg tggaatatct gacggtgttc
     7141 gccttcagca gcgaaaactg gcggcgtccc gccgacgaag tctccttcct gatgtcgctg
     7201 ttcctgaaga tgctggaacg cgagatcgcc agcatgcaca agaacaacat ccgcctgaag
     7261 atcatcggcg agcgtgcgga tttcgacgac aagctcaggc agaccatgca ggaagccgag
     7321 cagttgacgg caaacaatac cgggctgacc ctcaccatcg cggccaatta cggcgggcgt
     7381 tgggacatga tgcatgcggt gcaagccatg ctgaaagacc atccgcagtt ggcgcagggt
     7441 ttcaccgaag aggatttatc tccctatctt tccatgagcg atgcgcccga gcccgacctg
     7501 ttcatccgta ccggcggcga acagcgcatc agcaacttca tgctctggca gttagcctat
     7561 accgaattat attttaccga tgtgctgtgg cctgcgttcg gacgccgcga actggaggcg
     7621 gcgatcgcct cctatcagaa acgcgaacga cgcttcggcc ggaccagcga gcagttgacc
     7681 ggcgaaccaa tcaagggaga gaacggtgct taagttgcgg gtgatcacag ccattgtgct
     7741 ggtggtggta ttgctggctg cgttgttcct gttgccacca ttgggctggg cgttgctggt
     7801 cgtgctgatg gtgatgcagg gcacctcgga gtggtcgcgt ctggccggac tgagcggggc
     7861 cagggcgaac ctttattggg gactgacgct ggtgctgatg ctgggcttgc tgtggctgga
     7921 tgcggatgcg actgaaacgg cgcggcgcta tgtccatctg gcgatctata cgatatccgc
     7981 gttcctgtgg ctgatagtgg tgccgagctg gctgatcgtc ggctggaagg tcaagaatcc
     8041 cgtatggatg gcgctcacag gctgggcggt gttgatcccg accggtgtgg cgatgatgga
     8101 tctgcgcgag gccgctcccg gcccgtgggt gctgctgttc gtgatgggac tggtgtgggc
     8161 ggccgatatc gcggcttatt ttgccgggcg tcgtttcggc aagaacaagc tggcacccag
     8221 catcagtccc ggcaagacct gggaaggcgt gggcggcgcg atggtcggcg tgtcggtcta
     8281 tgtggtgctg gtgtggtggc tcagcccgta tttcgccacg cgcggaatct tgccaccgtt
     8341 gctgctggcc tcttggtggt gggtggcgct ggcggtgatc ggcgatctgt tcgagtccgc
     8401 gatcaagcgt caggccgggg tcaaggacag cggcgcgctg ctgcccgggc acggcgggtt
     8461 gctcgatcgc atcgatgcgc tgacctcgac cctgccgctg gccgccttcg tgctgttgtc
     8521 tcaggtggtg gcttaacaca tgcaaaatct aaccgtattg ggttctaccg gaagcatcgg
     8581 caccagcacg ctggacgtgg tggcgcggca tcccgagcgc taccgtgtcg tcgcgttgac
     8641 ggcgaattcg caggtcgatc tgctgttccg ccagtgcgag caattcaagc cggactatgc
     8701 cgtgctgctg gacgaggcct ccgccgccgg actgcgcaag cgcctgaatg atgccggtct
     8761 ggtcaccgag gtgctgtgcg gtgttgaagc tctggagcag gtctcggtgt tgccggaggt
     8821 ggatgcggtg atggcggcca tcgtcggtgc ggcgggcttg cgccccacgc tggcggcggc
     8881 tcgtgcgggc aagaaaattt tgctggcgaa caaggaaacg ctggtgatgg cgggcaacgt
     8941 gttcatggag gcggtgcgta ccagcggttc cgcactgttg cccatcgata gcgaacataa
     9001 cgcgatcttc cagagtctgc cgcgcggtta tgacggcgac atggcgcgca acggcgtcag
     9061 tcgcattctg ctgaccgcgt cgggcgggcc gttccgcaac acgccgctgg ccgaattgcg
     9121 caacgtcacg ccggagcagg cctgcgccca tccgaactgg agcatgggcc gcaagatttc
     9181 ggtggattcc gccagcatga tgaacaaggg gctggaagtg atcgaggcgc actggctgtt
     9241 caatgccagt gccgatgata ttcaggtggt ggtgcatccg cagagcgtga tccattcgct
     9301 ggtgcaatac gtggatggct cggtgatggc gcagctcggt aatccggaca tgcgcacgcc
     9361 gatcgcctat ggtctggcct accctgagcg tatcgacgca ggtgtcgcgc cgctcgatct
     9421 gttcaagatc gcgacgatga acttcgttgc gcccgacttc gagcgcttcc cctgtctggc
     9481 gctggcttat caggcattgc gcacggccgg tacggcccct gcaattctga atgcggcgaa
     9541 cgaggtggcg gtggacgcct tcctgaatcg ccgcatcgct ttcctcgata taccccgtgt
     9601 catcgcgacg gtgctggatg cgctgccggt caatgcggtc tcctgtctgg acgacgtggt
     9661 ggatgcggac gccgaggcgc gtcgcgcagc gctgcaacag attggcaatc tcgcagcacg
     9721 gcccgccgtg cccctacatc catgatcacc ttattggcct tcatcgctgc gatcgccctg
     9781 ctggtggtgt tccacgagct gggtcattat tgggtggcac ggcggtgcgg cgtcaaggtg
     9841 ttgcgttttt cgctgggctt cggccgggtg atgtacagca aacgctgcgc cagcggcacc
     9901 gagtgggtga tctctgcact gccgctgggt ggttacgtca aaatgctgga cgagagagag
     9961 gaggcggtca gtccggaaga gctggatcag gccttcaatc gcaagccggt gttgcagcgc
    10021 atggccatcg tggcggcagg gccgctggcg aattttttat tggctatcct gctgtactgg
    10081 gtgatgttca tccatggggt tccgggactg aagccggtac tgggcgaggt gccgtctggc
    10141 acccccgccg cagtggcgca gatgcaatcc ggcgaaacta tactcagcgt cgatggcatg
    10201 cttattccaa agtggcagga gttgcgctgg acgctgctgg atcttgcgtt gcaacagtcc
    10261 gaggtgcgga t
//
LOCUS       DASUIV010000267         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_812149, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000267 DASUIV010000000
VERSION     DASUIV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812149"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFW59_09680"
     CDS             <1..933
                     /locus_tag="VFW59_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5364527.1"
                     /translation="APIKAQVIHILNKPLPANVPSKTSRALLDIEDSSVVPIIRDPRK
                     AGEDSEAVFYFPGCGSERLFGQVGLATQAMLYEIGAITVLPPGYLCCGYPQNATGQGD
                     KAARITTDNRVLFHRVANTLNYLDIKTVVVSCGTCMDQLMQYQFDKIFPGCRLLDIHE
                     YLLEKGVKLETPTGLRYMYHDPCHSPMKTHQPLKVVNELMATAVPLTDRCCGESGTFA
                     VSRPDISTQVRFRKEEELRKGAEALRADGYEGEVKILTSCPSCQQGLSRYDNDSDTSS
                     EYVVVEMARHLLGPDWLADYVAKVNQGGIERVLL"
     gene            1012..2634
                     /locus_tag="VFW59_09685"
     CDS             1012..2634
                     /locus_tag="VFW59_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364528.1"
                     /translation="MLTLPLDPTDDRADPIFKDASACAKWLGQLQLTNLQLAHSLLYS
                     QLNELNRYPMRGMERLNTLELLRETIGYVQDDYAKKLIAKPLPLNESELMVFVSIVQL
                     WQAMVLGYQRCLQDYMAGDKQLAKHGALLCQRCLLYSGLEIFEHLRTGYEFEAKLWHQ
                     LHALYAFAEEQGFQLDEVTDPLNQKYPRSSCRSIYVKTLLSCYARPAELARSQLQLLD
                     NWLTQWSGLVTVEHSYSVSKGDAQPLTIDLDSTRGLYPVAQSVHRDSLRYLAMVPLSK
                     LLRVKTILLQQGQSPRQANLCEHCSSNDCIEFLTFLHSCWCEDHNVRFGERRPVAQHA
                     QICYRMEGIYAHLSGRAFKQPGRENALSGTARRQIESFGRVLQDAHNKELLEMGFPLE
                     TWQFENESISGARLTREDIFGGRLSYNQLLGLRPSDAETLMLGAVAWANVTRDGKLQI
                     GVRYLPGAAEPVSMKAVGSGTGLPERYFPAFLLPSIQALKIPASLIVPRDWFQPDRVV
                     EILHLNGEADDLKMGFSVERGLDYERVSFVPV"
     gene            complement(2692..>3621)
                     /gene="phoR"
                     /locus_tag="VFW59_09690"
     CDS             complement(2692..>3621)
                     /gene="phoR"
                     /locus_tag="VFW59_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEX5364529.1"
                     /translation="CNPLAQQHFGLEADRDFMQDITYLVRQPEFIEYLNAADFGEPLV
                     MSPARHDDMVLSIKFIPYGDDKRLMISRDITQLERIEAMRRDFVANVSHELRTPLTVV
                     NGYVENLIDMPDISRADQNRALQLMAEQTRRMESLVSDLLTLSRLESEHNPLREETVN
                     MGELLNEVYREGLSLSNGRHTLSVEEARSINLSGNRDELHSAFSNLVSNAIRYTPEGG
                     KILLRCGERSGQAVFSVQDSGIGIAAQHLPRLTERFYRIDRSRSRETGGTGLGLAIVK
                     HIAIRHQAKLEVMSEEGKGSTFSLVFPTARVVR"
BASE COUNT          687 a         1109 c         1129 g          696 t
ORIGIN      
        1 gcgccgatca aggcgcaggt gatccatatc ctgaacaagc cgctgcccgc gaatgtgccc
       61 tccaagacct cgcgcgcgct gctcgacatc gaggacagca gcgtggtgcc gatcatccgc
      121 gacccgcgca aggccggtga agattcggag gcggtgttct acttcccggg ctgcggttcg
      181 gagcgattgt tcggtcaggt cggtctggcg acccaggcga tgctgtatga aatcggcgcg
      241 atcaccgtgc tgccgccggg ctatctgtgc tgcggctatc cgcagaatgc cacggggcag
      301 ggcgacaagg ccgcacgcat caccaccgac aaccgcgtgc tgttccatcg cgtcgccaac
      361 acgctgaact acctcgacat caagaccgtc gtggtgtcct gcggcacctg catggaccag
      421 ctgatgcaat accagttcga caagatattc cccggctgcc gcctgctgga tattcacgag
      481 tatctgctgg agaagggcgt gaagctggaa acgcccaccg gcctgcgcta catgtaccac
      541 gatccctgcc actcgccgat gaagacccat cagccgctga aggtggtgaa cgagctgatg
      601 gcgactgcgg tgccgctcac cgaccgctgc tgcggcgaat ccggcacctt cgcggtatcg
      661 cgcccggaca tctcaaccca ggtgcgtttc cgcaaggaag aggagttgcg caagggggcc
      721 gaggcgttgc gtgccgacgg ttacgagggc gaagtgaaga tactcacctc ctgcccgtcc
      781 tgtcagcaag gcctgtcgcg ttacgacaac gacagcgaca cctcctccga atatgtggtg
      841 gtggagatgg ccagacatct gctcggtccg gactggctgg cggactatgt cgcgaaggtc
      901 aatcaaggcg gcatcgaaag agtgttgctg tgataatctc cggggctatg ccgcgcttga
      961 cggaaaatca tccctcgtgg aaagtagcgg cctgagaccc aaaaacaaac catgctgacc
     1021 ctgcctctcg acccgaccga tgaccgcgcc gatccgatct tcaaggatgc ctcggcttgc
     1081 gcaaaatggc tggggcagct gcaactgacc aatctgcaac tggcgcacag cctgttgtac
     1141 agccagctca acgaactcaa ccgctatccg atgcgcggta tggaacggct caatacgctg
     1201 gagctgctgc gcgagaccat cggctatgtg caggacgatt acgccaagaa acttatcgca
     1261 aagccactgc cgctgaacga gagcgagctg atggtgttcg tgtccatcgt gcagctctgg
     1321 caggccatgg tgctgggcta tcagcgctgc ctgcaagact acatggcggg cgacaaacag
     1381 ctcgccaaac acggcgcact gttgtgccag cgctgcctgc tgtatagcgg tctggaaatc
     1441 ttcgagcacc tgcgcaccgg ctacgaattc gaggccaagt tgtggcacca gctgcacgcg
     1501 ctctacgcgt ttgccgaaga gcagggtttc cagctggatg aagtgaccga tccgctcaac
     1561 cagaaatatc cgcgcagcag ctgccgcagc atctacgtca agacgctgct gtcctgttac
     1621 gcccgtcccg ccgagcttgc ccgttcgcaa ctgcagctgc tggacaactg gctgacccag
     1681 tggagcgggt tggtgaccgt ggagcacagc tacagcgtca gcaaggggga tgcgcagccg
     1741 cttaccatcg atctggacag cacgcgcgga ctctatccgg tcgcccagag tgtccatcgg
     1801 gattcgctgc gctatctggc catggtgccg ctgtccaagt tgctgcgggt gaagacgatc
     1861 ctgctgcaac agggccagtc cccgcgtcaa gccaatctgt gcgaacactg cagcagcaac
     1921 gactgcatcg agttcctcac cttcctgcat agctgctggt gcgaagatca caatgttcgc
     1981 ttcggcgagc gccgtccggt cgcgcagcat gctcagatct gctacaggat ggagggcatc
     2041 tatgcccatc tgtctggccg ggccttcaaa cagccgggcc gcgaaaatgc gctcagcggt
     2101 accgcgcgcc gacagatcga atcctttggc cgcgtgctgc aggatgcgca caacaaggaa
     2161 ttgctggaga tgggcttccc gctggaaacc tggcagttcg agaatgagag catctccggc
     2221 gcgcggctga cgcgcgaaga tatcttcggc ggacgtctga gctataacca gttgctcggt
     2281 ctgcgcccca gcgatgccga aaccctgatg ctgggggcgg tggcctgggc gaacgtgacc
     2341 cgggacggca agttgcagat cggtgtgcgt tacctgccgg gcgctgcgga gccggtcagc
     2401 atgaaggccg tcgggagcgg tacgggtctg cccgaacgat atttcccggc ttttctgttg
     2461 ccgagcattc aggcgctaaa gatcccggcc agcctgatcg tcccgcgcga ctggttccag
     2521 ccggaccgtg tcgtggaaat cctgcacctg aatggcgaag cggacgacct gaaaatgggc
     2581 ttcagcgtag agcggggcct cgattacgag cgcgtcagct tcgttcctgt gtagcgcccg
     2641 gcccctcaag gcccagccag cgcgagcttc ttcgcgcagg ggacagcgtt cctagcgtac
     2701 gacgcgggcg gtcgggaaca ccagcgagaa ggtgctgccc ttgccttctt cgctcatgac
     2761 ttccagcttg gcctgatggc gtatcgcgat gtgtttgacg atggcgagtc ccagacccgt
     2821 gccgccggtt tcgcgcgagc ggctgcggtc gatccggtag aagcgctcgg tcaggcgcgg
     2881 caggtgctgc gcggcgatgc cgatgccgct gtcctgtacc gagaacaccg cctgcccgct
     2941 tcgctcgcca cagcgcagca ggatttttcc gccttcgggg gtgtaacgga tcgcgttgct
     3001 gaccagattg ctgaatgcgc tgtgcagttc atcgcggttg ccggacaggt tgatgctgcg
     3061 ggcctcttcg acgctcaggg tgtggcgccc gttgcttagc gacaggccct cgcgatagac
     3121 ctcgttgagc aactcgccca tgttgaccgt ctcctcgcgc agcgggttgt gctcgctctc
     3181 cagacgcgac agcgtcagca ggtcggatac caggctctcc atgcgccggg tctgttctgc
     3241 catcagttgc agggcgcgat tctgatccgc gcgactgatg tcgggcatgt cgatcagatt
     3301 ctcgacatag ccgttcacca cggtgagcgg ggtgcgcaat tcgtgcgata cattggccac
     3361 gaagtcgcgc cgcatcgctt cgatgcgttc cagctgggtg atgtcgcggc tgatcatcag
     3421 ccgcttgtcg tcgccatagg ggatgaattt tatcgacagc accatgtcat cgtgacgtgc
     3481 cggtgacatc accagcggct cgccgaaatc ggcagcattc aggtattcga tgaactccgg
     3541 ttggcgcacc agataggtga tgtcctgcat gaaatcgcga tcggcttcga ggccgaaatg
     3601 ctgctgggca agcgggttgc a
//
LOCUS       DASUIV010000268         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1370745, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000268 DASUIV010000000
VERSION     DASUIV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370745"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..1482
                     /locus_tag="VFW59_09695"
     CDS             250..1482
                     /locus_tag="VFW59_09695"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5364530.1"
                     /translation="MALIVQKYGGTSVGSPDRIKNVARRVAKFKAQGHQVVVVVSAMS
                     GETNRLVGLAHEIQQNPDPRELDVIISTGEQVTIGLLAMALKEIGLKAKSYTGGQVKI
                     LTDSAFTKARIVSIDEQNIRADLASDHVVVVAGFQGMDEDGNITTLGRGGSDTTGVAI
                     AAALRADECQIYTDVDGVYTTDPRVVPEARRLKTITFEEMLEMASQGSKVLQIRSVEF
                     AGKYKVKLRVLSSFAEEGEGTLITFEDEEKMEQAIISGIAFNRDEAKITVLGVPDKPG
                     IAYQILGPVSDANIDVDMIIQNVSEDGTTDFSFTVHRNEFAKAMEILKNQIQPHIGAR
                     QVVGDNKAAKVSVVGVGMRSHVGIASKMFRTLAEEGINIQMISTSEIKISVVIDEKYL
                     ELAVRVLHKAFNLDQEDA"
     gene            1537..1630
                     /locus_tag="VFW59_09700"
     tRNA            1537..1630
                     /locus_tag="VFW59_09700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1571..1573,aa:Ser,seq:gct)
     gene            1658..1734
                     /locus_tag="VFW59_09705"
     tRNA            1658..1734
                     /locus_tag="VFW59_09705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1692..1694,aa:Arg,seq:acg)
     gene            1866..1942
                     /locus_tag="VFW59_09710"
     tRNA            1866..1942
                     /locus_tag="VFW59_09710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1900..1902,aa:Arg,seq:acg)
     gene            2320..2643
                     /locus_tag="VFW59_09715"
     CDS             2320..2643
                     /locus_tag="VFW59_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX5364531.1"
                     /translation="MITLTPNALSAVEKFIKGSSAPVAGLRIAISGGGCSGFQYGMSL
                     EEVKNEDDTVLDYGAVTLLVDPLSAPLLEGVTVDFVDSLNGSGFKFENPNASASCACG
                     SSFSA"
     gene            2654..3541
                     /gene="nifU"
                     /locus_tag="VFW59_09720"
     CDS             2654..3541
                     /gene="nifU"
                     /locus_tag="VFW59_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HEX5364532.1"
                     /translation="MWDYSDKVKEHFFQPRNAGVLEDANGVGDVGSISCGDALRLMLK
                     VEPETDIILDAHFQTFGCGSAIASSSALTEMVKGKTLDEALKVSNQDIADYLDGLPPE
                     KMHCSVMGREALQAAVANYRGEVWSDDHEEGALICKCFAIDAVMIEDTIRANNLSSVQ
                     DVTNYTKAGGGCSSCHEGIEEILVKVMSERGESFKPGVQPTEQASTSGMTNLQRIRKI
                     EAVLESVRPTLQRDGGDIELVDVDGKTIYVNMTGACAGCQMEAITLQGIQQKLMEELK
                     EFIKIVPTKVSSLTRAMKG"
     gene            3544..>4529
                     /gene="nifS"
                     /locus_tag="VFW59_09725"
     CDS             3544..>4529
                     /gene="nifS"
                     /locus_tag="VFW59_09725"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HEX5364533.1"
                     /translation="MTGIYFDNNATTRVDPSVVEAMLPYFTEQFGNPSSMHSFGNKVG
                     LAIKKARMQVQELLGAEHDSEIIFTSCGTESDSTAILSALKAQPERREIITTTVEHPA
                     VLTLCTWLEKEGYKVHYLPVDDKGRLDLDEYRKLLTDQVAVVSVMWANNETGTFFPVV
                     EMAELANAAGAMFHTDAVQAVGKVPLNLKDTKIDMLSVSGHKLHAPKGIGVLYLKRGV
                     RFRPLLRGGHQERGRRAGTENTTSIVGLGKAAEMALEAMDFENTEVARLRDKLEAGIL
                     ARVPRSFVTGNPDDRLPNTSNIAFEFIEGEAILLLLNKFGIAASSGSACTSGS"
BASE COUNT         1006 a         1278 c         1363 g          882 t
ORIGIN      
        1 tgtcggcggt gcgcgggaag atggctgtca tgagatcggg taaggcgggt gttcggcgtc
       61 tgcggagttt agcatccagt tgatgcgaga gcccgatcga acgacagcca tccggttaac
      121 ccgggcgaag atgctgggta agtcatgggt tgcctgcggg acagcagcac gttattgccc
      181 acctcgtttt tttacggcaa ttacgctaga atcgcgtcca ctttaaaaca agcctgagat
      241 ggaaaaatcg tggcactgat agttcagaag tatggtggta cctcggtcgg cagtcccgat
      301 cgcatcaaaa acgtagcgcg tcgcgttgcc aaattcaagg cgcaggggca tcaggttgtg
      361 gtggtcgtat ccgcgatgag cggcgagacc aaccgtctgg tcggtctcgc gcatgagatt
      421 cagcagaacc ctgacccgcg cgaactggat gtcatcatct ctaccggcga gcaggtcacc
      481 atcggcctgc tggcgatggc gctcaaggag atcgggctga aggccaagag ctacacaggc
      541 ggtcaggtca agattctcac cgatagcgcc ttcaccaagg cccgcatcgt cagcatcgac
      601 gagcagaaca tccgcgccga tctggcgagc gatcacgtgg tcgtggtcgc cggctttcag
      661 ggcatggacg aagacggcaa catcaccacg ctgggtcgcg gcggttctga caccaccggg
      721 gtggcgatcg ccgccgcgct gcgcgccgac gaatgccaga tctacaccga cgtggacggc
      781 gtttacacca ccgacccgcg cgtcgtgccc gaggcgcgcc gcctgaagac catcaccttc
      841 gaagaaatgc tggagatggc gagccagggc tccaaggtgc tgcagatccg ttcggtggaa
      901 ttcgccggca aatacaaggt caagctgcgc gtgctctcca gcttcgcgga agaaggcgaa
      961 ggcacgttga tcacttttga ggatgaagaa aaaatggaac aagcaatcat ttccggaatc
     1021 gcgttcaacc gcgacgaagc caagatcacc gtgctgggcg tgccggacaa gccgggtatc
     1081 gcctatcaga ttcttgggcc ggtctccgat gccaatatcg acgtggacat gatcatccag
     1141 aacgtcagcg aagacggcac caccgacttc tcgttcaccg tgcaccgcaa cgagttcgcc
     1201 aaggcgatgg agattctgaa gaaccagatc cagccgcaca tcggcgcgcg ccaagtggtc
     1261 ggcgacaaca aggcggccaa ggtgtccgtg gtcggcgtcg gcatgcgttc gcatgtcggc
     1321 atcgccagca agatgttccg caccttggcg gaagagggca tcaacattca gatgatctcc
     1381 acctccgaga tcaagatctc ggtggtcatc gacgagaaat atctggagct ggccgtgcgt
     1441 gttttgcaca aagccttcaa cctggaccag gaagacgcgt aaatcgcaca atcagtttga
     1501 cctttccgga cgtttgcact aatatgcgcg gcttccggag aggtggccga gaggtcgaag
     1561 gcacgcccct gctaagggcg catacgagct taaacttgta tcgagggttc gaatccctcc
     1621 ttctccgcca aggtaactgt agtaatgttt taaagatgcg cccgtagctc agctggatag
     1681 agtacttggc tacgaaccaa ggggtcagga gttcgaatct cttcgggcgc accaaaaatt
     1741 acacgggtta gatcagaaat ggtctagccc gtttttatca gacgccctgc aaagggagca
     1801 tgaaagcggt ttagacagcg agtattttgc gtctccgcca aaatatcaga agtaaagttt
     1861 cacatgcgcc cgtagctcag ctggatagag tacttggcta cgaaccaagg ggtcaggagt
     1921 tcgaatctct tcgggcgcac caataatcaa agggttaggc agaaatgctt agcccttttt
     1981 ctttcctgaa ggagttttga agggactcag tcctgaagga actttgaagg gactcggcct
     2041 tttccctgaa cagcatctcc aagcttgcgt tcgtcctgtt gcgtgcgtgc aagcttgccc
     2101 atgtatggcg caggttgtgc cagcggaaat tccccataag ttattcacga agtggttgtt
     2161 gcaaacccta caaccaaccc ttgtggtcat gtttcagaca tgggggttga gcgtttgctt
     2221 ctatcaatat cccaattaaa caagtcatta cctgttttcg ggttgtttgg cacagagttt
     2281 gcggaatatc aggcacaaac accctgatag gagcgaacca tgatcaccct gacacccaac
     2341 gcactctccg ccgtggagaa gttcatcaaa ggatcgagcg cccctgtggc cggactgcgc
     2401 atcgccatct cgggcggcgg ctgctccggc ttccagtacg gcatgagtct ggaagaagtc
     2461 aaaaacgaag acgacaccgt gctggattac ggcgcagtga ccctgctggt cgatcccttg
     2521 tcggctccgt tgctggaagg cgtgacggtc gatttcgtcg atagcctgaa cggcagcggt
     2581 ttcaagttcg aaaacccgaa cgccagcgcc agctgcgcgt gcggttcttc attctcggca
     2641 taaggagcgc gtcatgtggg actactcgga caaggtcaag gaacatttct ttcaaccccg
     2701 taatgcaggc gtgctggaag acgcgaacgg cgtgggcgat gtcggctcga tcagttgcgg
     2761 tgatgcgttg cggctgatgc tcaaagtcga gccggagacc gacatcatcc tcgacgcgca
     2821 tttccagact ttcggctgcg gctccgccat cgcgtcttcc tctgcgctga ccgagatggt
     2881 caagggcaag acgctggacg aggcgctgaa agtcagcaac caggacatcg ccgactacct
     2941 cgacggcctg ccgccggaga agatgcactg ctcggtgatg gggcgcgagg cgctgcaggc
     3001 tgccgtcgcg aactatcgcg gcgaagtatg gagcgacgat cacgaagagg gcgcgctgat
     3061 ctgcaaatgc ttcgccatcg acgcggtgat gatcgaagac accatccgcg ccaacaacct
     3121 gtcgtcggta caggacgtga ccaactacac caaggccggc ggcggctgct cctcctgcca
     3181 tgagggcatc gaagagatcc tcgtgaaggt gatgagcgag cgcggcgaga gcttcaagcc
     3241 cggtgtgcaa ccgaccgagc aggcttcgac ttcgggcatg accaacctgc agcgcatccg
     3301 caagatcgag gcagtgctcg aatcggtccg tccgacgctg cagcgcgatg gcggcgatat
     3361 tgaactggtc gatgtggacg gcaagaccat atacgtcaac atgaccggag cctgtgccgg
     3421 ttgccagatg gaggcgatca cgctgcaggg catccagcag aagctgatgg aagagctgaa
     3481 agagttcatc aagatcgtgc cgaccaaggt cagttcgctc acccgcgcaa tgaaaggctg
     3541 atcatgaccg gcatctattt cgacaacaat gcgacgaccc gggtcgatcc ctcggtggtg
     3601 gaagccatgc tgccgtactt caccgagcag ttcggcaatc cctcgtcgat gcacagcttc
     3661 ggcaacaagg ttggccttgc gatcaagaag gcgcgcatgc aggtgcagga actgctcggc
     3721 gcggaacacg actccgagat catcttcacc tcctgcggca cggagtccga ctccaccgcc
     3781 atcctgtccg ccctgaaggc gcagccggaa cgccgcgaga tcatcaccac cacggtcgag
     3841 catccggcgg tgctgacgct gtgcacctgg ctggagaagg aaggctacaa ggtgcattac
     3901 ctcccggtgg acgataaggg gcgcctcgat ctggatgaat acaggaagct gctgaccgat
     3961 caggtcgcgg tcgtgtccgt gatgtgggcg aacaacgaga ccggcacctt cttccccgtg
     4021 gtcgagatgg ctgagctggc gaatgccgcc ggtgcgatgt tccacaccga cgccgtacaa
     4081 gcggtgggca aggtgccact gaatctgaaa gacaccaaga tcgacatgct ctccgtgtcc
     4141 ggtcacaagc tgcatgcccc caagggcatc ggtgtgctgt acctcaagcg cggtgtgcgt
     4201 ttccgtccgc tgttgcgcgg aggccatcag gaacgcggtc gtcgtgccgg caccgagaac
     4261 accacctcca tcgtggggct gggcaaggcc gccgagatgg cgctggaggc gatggatttc
     4321 gagaacactg aggtggcacg cctgcgcgac aaacttgaag caggcatatt ggcccgtgtg
     4381 ccgcgttcgt tcgtgacggg caatccggat gaccgtctgc ccaacaccag caacatcgcg
     4441 ttcgagttca tcgagggcga ggccatcctg ttgctgctga acaaattcgg tatcgcggca
     4501 tccagcggtt ccgcctgtac ctcgggttc
//
LOCUS       DASUIV010000269        13847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_69577, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000269 DASUIV010000000
VERSION     DASUIV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 13847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13847
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69577"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VFW59_09730"
     CDS             <1..1740
                     /locus_tag="VFW59_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364534.1"
                     /translation="FVLLGSALLLDGKTRRSGVSISALSLLMALLALAAGLVYLYDTD
                     NLYGLGHIMQLAAHTALAFIVLAAGLLCSLPEHGVVALLRRRDSGGALARRLLPVTLL
                     LPVLIGWLKLEGVRAGLYEPDFGMVLVALVYIVIFGLSVIWVAHFLSRIDAERERMTA
                     NLVRNETQLRTLVGTIPDLIWLKDTQGVYLVCNPAFEHFFGVKADVIVGKTDYDFVAR
                     EQADFFRAKDRAVMAARGPLRNEEWLTLSDGQRILAETTKAPTFDPQGNLTGVLGIAR
                     DITRRHQSEETLRFAYAEAERLLEEANQAKLALISALEESKAASAALHQSEEKFRLLS
                     AVLEQRVQERTAKLEAVNQELETFSYSVSHDLRAPLRAIAGFVELLRKHSYREIDDKG
                     RHYMDVISESAIQMARLIDDILTFSRIGRVEMTKTLVSLDQVLTEVLHTLQPQIAERK
                     IEWHIGPLPQVQGERTMLSLVLLNLLSNALKFTGTRETARIEVGQLDSAADAGETICY
                     VRDNGVGFDMQYVSKLFGLFQRLHAQEQFEGTGVGLANVQRIIQRHGGRVWAESRVDE
                     GATFYFALPNSGG"
     gene            1742..2197
                     /locus_tag="VFW59_09735"
     CDS             1742..2197
                     /locus_tag="VFW59_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364535.1"
                     /translation="MKALARILLVEDNPNDVEMTLDAFNECNLANEVVVVKDGQEALD
                     YLQCQGGFASRLHGNPAVILLDLKLPKIDGMQVLRRIRADEELKLIPVVIMTSSREER
                     DVAEGYRLGVNAYVVKPVEFSDFVDAVKGLGVFWAVLNQPPLGCANRDV"
     gene            2199..6350
                     /locus_tag="VFW59_09740"
     CDS             2199..6350
                     /locus_tag="VFW59_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5364536.1"
                     /translation="MGLTDGKIQDRLRVLHLEDNPNDRELIRSELEAEWPQVELSGVA
                     TRAAFLQALDEFKPDLILSDYRLPDYDGRSALTHVRQHHPEIPVIMVSGALGETEAVE
                     LVKMGARDYVMKGELQRLPGAVKSALSEEYGIRARKAAEKALWQSEADLRALVESSPI
                     AMIVDVGMGADQKVVMMNRQFIKLFGYTMADVPDIHHWWQLAYPDEQYREKLKAEWAD
                     QAIRKHGAIEPKDVTVTCKDGSSRYVRISLASAGNRNIVTFEDLTAGKLLEDALQEAV
                     HRNQVMMETVNDAIVCIDQAGIIYLWNLKAEEMFGYSAAEAIGQDLHSLITPARYYES
                     STHALGKFAQTGTGPIIGVTRQIDAKRKDGSEFPVELSVSAMSIQGTWHATGIIRDIT
                     ERKQAESRIARLVQLYAALSQCNQAIVRSKNPTDLFEQICHAAVTFGGFKMAWVGLID
                     PGTGMVKPVFSFGEGAEEYLRGIQISISIESEFGQGPTGCAIRENKPFWCQDFMSDPL
                     TSPWHDRAIALGWKSSAALPLSREGAVIGAFTLYGDEVNQFDADERKLLVEMAEDVSY
                     ALDNFRHETERKQTEDALVESNNLLKAVIQTSPMRIFWKDRELRYLGCNAAFARDAGE
                     KSPAEIVGKDDYQLGWKAQASLYRADDNQVMESGIPMLSYDELQSTPDGRNIWLRTSK
                     VPLRNAANEIIGVLGIYEDITERKLVELALQRSEADLNRAQAVAQVGSWNLDILSNRL
                     EWSAEACRIFGIPGQEAVDLDTFISTIHPDDREMVLKAWEAAIAGEPYDIEHRVVADG
                     VTRWVRERAVIERDAAGHALFGVGTTQDITERRLAEDQLRKLSLAVEQSPNSIVITDL
                     NANLEYVNEAFLWTTGYSREEVIGKNPRILHSDKTPRETYDDMWAHLARGDSWKGEFT
                     NRRKNGGEYIESIFVSPVRDSDGTVTHYVGIKEDITERKKVEKSLQESEEKFRVMSAS
                     AQDGIVMIDNDGNISFWNRAAENIFGYSASEALGKNLHLLLSPVRFHEASDKGFAQFQ
                     DSGKGAVIGKTLELMALHNSGKEFPLELSLSAVNISGLWQAIGIVRDISERKENQENM
                     LAQYSHVAGVNAQLMAANKQLEQTQKQLLQSEKMAAVGLLAAGVAHEINNPVGYVNSN
                     LGTLEKYLADIFVDIDKYEAAEAVMAGDNLTLDEVRQLKARIDLAYLREDIKALLAES
                     HQGLERVKKIVLDLKEFSHSDAQDQWVWADVHHGLDTTLSVVWNELKYKCEVVKEYGD
                     LPKIYCLPSQLNQVFMNLLVNAAQAIEVRGKITLRTGLEGDRIWVEVSDTGHGISPEH
                     IPRLFDPFFTTKPVGQGTGLGLSVSYNIVEKHHGSFEVHSEIGRGTTFRVWLPVQQSD
                     NKEKA"
     gene            6347..7666
                     /locus_tag="VFW59_09745"
     CDS             6347..7666
                     /locus_tag="VFW59_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5364537.1"
                     /translation="MTQDTAAPGNVPATLLFIDDEANILAALKRLFRPLGYNILTAES
                     GAEGLALLEKENIDLVISDMRMPNMTGAELLEQARNKWPEVIRILLTGYSDLSSTIAA
                     INRGEIYRYISKPWDDNDIVLIVHDALERKQLLAEKQRLEALTQQQNEALKTLNASLE
                     DKVQQRTAELRTAMNSLARAHDRLKQDYFSTIQVFANLMELRKGTMAGHSRRIARLCH
                     DIALKMGLPETDIHAIETAALLHNIGKISLPDRLLDTPYLALPYADRTEFDKHPLRAA
                     AALMALEPLAQVSELIRHHREHYDGVGNRSNVRGNDIPLGARILLVASDYDALQEGLL
                     GQDKLTPNQALDTIVKGGGMRYDPNVVDVFREVVGQSVQQGRVEAEFQVSSSQLHEGM
                     LLTRDVLSRDGMLLLLKDTMLNAGHLREIREFESSTGESLTIFTHLM"
     gene            7676..8602
                     /locus_tag="VFW59_09750"
     CDS             7676..8602
                     /locus_tag="VFW59_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364538.1"
                     /translation="MNSYRILLLDDDDFVLAALRRELLNKPYVGHDSLEIEAFVSPAA
                     ALARAREPEGYFDLVISDIRMPEMSGIDFLKAFGEIQPDAARIVLSGDTDMPELMRAI
                     NEAHIDFFIAKPWHEYDLKSALWQTLRQYDLRCENRMLARQYLEHFQLPHQFRRKSCY
                     QLMVVDDDQNVLHALQRELGAAHAEGRAFGQYQLEVHPFESVNAALQAARELQFDVVI
                     SDYAMPGMNGVEFLHLFREAQSNAVRILLSGNADMEVFVNAVNTAGVSYFIAKPWRDY
                     ELRGAIDRALTYRELVLENRFLAEMLRLRGQH"
     gene            8616..9542
                     /locus_tag="VFW59_09755"
     CDS             8616..9542
                     /locus_tag="VFW59_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364539.1"
                     /translation="MYRILIVDDEQNVLNALRRELQGEYAVETFASPEEALAHSLHAQ
                     FDLVIADYQMPGMNGIQFLRQFGKLQPDAARLVLSGGTDFAALIGSVNETHIYRFIAK
                     PWDKTELAVTLAQALAHRATLLENRRLAELCRKERQWRRAQDPDKLYQVLVVDDEPNV
                     LSAVKRDLTVHERFDDLHAAMRHTDNPGAPVEHHDFRFNVHTASSPLQALEQARQVSF
                     DVVIADYLMPEMDGLRFLEAFRQLQPDTARILFSGHADREVLVKAINGSEIYGYIEKP
                     WREYVLKNTVSQAVAYRNLLRESRHLAEQMAP"
     gene            complement(9599..9688)
                     /locus_tag="VFW59_09760"
     tRNA            complement(9599..9688)
                     /locus_tag="VFW59_09760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9652..9654),aa:Ser,seq:tga)
     gene            complement(9815..10261)
                     /gene="queD"
                     /locus_tag="VFW59_09765"
     CDS             complement(9815..10261)
                     /gene="queD"
                     /locus_tag="VFW59_09765"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEX5364540.1"
                     /translation="MLITRRLEFDAGHRIPNHNSQCRHMHGHRYAIEITLSGELITTE
                     GQSEQGMVMDFSDVKRIAKEKLVDAWDHAFLVYRNDRKVCDFLATLPEHKTVVLDVVP
                     TAEHLAQVAFDILNAAYTDLYGNNLRLERVRLYETPNNWADCERTS"
     gene            complement(10301..12232)
                     /locus_tag="VFW59_09770"
     CDS             complement(10301..12232)
                     /locus_tag="VFW59_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX5364541.1"
                     /translation="MSKSLLYGLLLSILMVAAPHAEHLPPWVSALCAALLIWRAYLAR
                     SGNPLPPRWLLMTLTLASAGGILIEFHTLFGREAGVTLLILLTTLKFMELRSARDATV
                     LVYLACFIIITNFFYSQSIPTALYLLATLLVIVTTWVHLHGQSMPLGPRLKVATTLLL
                     QAIPLTLVLFVLFPRVQGPLWGMPNDAFASSGLDDKMSPGSFGRLSLSDEVAFRVVYH
                     GTPPRRDQMYWRGPVLWNFDGITWTQGRAARTLAPQFTELGQPIAYSVTLEPHNKTWL
                     FALDLPDSISVPVRLTHDFQIVQQEPLKTRLRYEARSHLTYHANLRESERQIQRALQL
                     PAGLNPRAQQLAEQWRAEGANDAAVVGKALNYFNRENFGYTLEPPPLPGLNNIDAFLF
                     ETRQGFCEHYAGSFVFLMRAAHIPARVVTGYQGGEYNDVGGYYIVRQSDAHAWAEVWL
                     SGQGWVRIDPTASISPARIERGLSAAVPNNAALPFMARNPPQWLSKLRLNWDALSNQW
                     NLWVLGYNSERQFAFLTRLGMEDISWQKLALNMSLGLGLVIAAFALFMLRHLIQPQPD
                     KVQAAWLKLCRKLEKSGLPRKPHEGPQDYATRVAAARPALADSIRDLAARYSALRYGN
                     MQDEQARREFLKRAATCKI"
     gene            complement(12229..13281)
                     /locus_tag="VFW59_09775"
     CDS             complement(12229..13281)
                     /locus_tag="VFW59_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5364542.1"
                     /translation="MLTALKQKFRIWLFRPKIEHGTVTLTQRRIFILPTRQGIGLAFV
                     LVLMLLGDINYSLSLGYVLTFLLAMMAVMSMLHAFRNLAHLEARAGRVDAVFGGETAR
                     FPLHFRNPSKLARYQLMLRGADGEPVGFDVPAQQDCEVALPLAARQRGWLTPGRLTLY
                     TEFPLGLFHAWSYLHFDTRCLVYPKPLAEAALPPGDAPDGTGKRSVTGDDDFAGLRGY
                     VEGDALPRIAWKAYARERGLQVKQFSMQIGEELWLDYAATPETDIEGKLSHMTRWVLD
                     AETQGLRYGLRLPNGELPPADGQSHRDECLRRIALFELPQQERPLPTPSPENGRGGPV
                     TPLSHSWERGRARGKP"
     gene            complement(13295..13543)
                     /locus_tag="VFW59_09780"
     CDS             complement(13295..13543)
                     /locus_tag="VFW59_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX5364543.1"
                     /translation="MKLPRDLSGAQLIKALERLNYKPTRQSGSHVRLTGSLPNKEHHL
                     TVPLHDPLRIGTLAAILADVATHHGMTRDAVIARLFGK"
     gene            complement(13540..>13847)
                     /locus_tag="VFW59_09785"
     CDS             complement(13540..>13847)
                     /locus_tag="VFW59_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364544.1"
                     /translation="GQRYAGARTDRGGRDPVISGDARMHASPVERMTMNEIMFVVEEA
                     PEGGLIARALGESIFTEADDVESLHRQVRDAVHCHFEEGKEPKIIRLHFTREEVLAV"
BASE COUNT         2786 a         3785 c         4497 g         2779 t
ORIGIN      
        1 tttgtgttgc tgggctcggc attgctgctg gatggcaaga cccggcgcag cggggtgagt
       61 atttccgccc tttccctgtt gatggcgctt ttggcactcg ctgccgggct ggtctatctc
      121 tacgataccg acaacctcta cggtttgggg catatcatgc agctggctgc gcatacggcg
      181 ctggctttta ttgtcctcgc cgccggtctg ctctgttcct tgccggagca cggtgtggtc
      241 gcattgctgc gtcgtcgcga cagcggcggc gcgctggccc gccgcctgct gcctgtcacg
      301 ttgttgctgc cggtcttgat cggctggctg aagctggaag gggttcgtgc ggggctgtat
      361 gagccggatt ttggcatggt gctggtcgcg ctcgtctata tcgtcatctt tggcctatcg
      421 gtgatatggg tcgcccattt cctgtcgcgg atcgatgccg agcgcgagcg catgacggcg
      481 aacctggtca ggaatgaaac acagttgcgc acgttggtcg gcaccattcc cgacctgatc
      541 tggttgaaag atacgcaggg tgtttatctg gtctgcaacc cggccttcga gcattttttc
      601 ggagtgaagg ccgatgttat cgtcggcaag accgattacg acttcgtcgc gcgcgaacag
      661 gcggatttct tccgtgccaa ggatagggcg gtcatggcgg cgagggggcc gctcagaaat
      721 gaggaatggc tgaccctgtc ggatgggcag cgcatcctgg cggagaccac gaaagcgccg
      781 acgtttgatc cgcagggcaa tttgaccggc gtgctgggca tcgcgcgcga catcacccgg
      841 cgtcaccagt cggaagagac gctccggttc gcttatgccg aggccgagcg cctgctggaa
      901 gaggcgaatc aggcaaagct ggcgctgata agtgcgctcg aagagagcaa ggcggccagc
      961 gccgcattgc atcaaagcga ggagaagttc cgtctgctca gtgccgtgct ggagcagcgc
     1021 gtgcaggagc gcacggccaa gctggaggcg gtcaaccagg agttggagac tttttcctat
     1081 tcggtatcgc acgatctgcg cgctcccttg cgcgcgatcg ccggttttgt cgaattgctc
     1141 agaaagcaca gctatcggga gatcgatgac aagggcaggc actacatgga tgtgatttcc
     1201 gagtcggcga tacagatggc caggctgatc gacgacatcc tgaccttctc gcgcatcggg
     1261 cgggtcgaga tgacgaagac gctggtctcg ctggatcagg tgctgactga agtgttgcat
     1321 accctgcagc cgcagattgc ggaacggaag atcgaatggc atatcggccc cttgcctcaa
     1381 gtgcaaggcg aacgcacgat gctgtcgctg gtgctgttaa atctgctgtc caatgcgctg
     1441 aaattcaccg gcacgcggga aaccgcgaga atcgaagtcg gccagcttga cagcgctgcc
     1501 gatgcggggg aaaccatctg ctatgtgcgc gacaatggag taggattcga catgcagtat
     1561 gtgagcaaat tgttcgggct gtttcagcgc ctgcatgcgc aggaacagtt cgagggaacc
     1621 ggggtcggat tggctaatgt gcagcgcatc atccagcggc acggtgggcg ggtttgggcg
     1681 gagagccggg tggacgaagg ggcgacgttc tatttcgcac tgccgaattc aggagggtga
     1741 catgaaagcg ctggcacgga tactgttggt cgaggacaac ccgaacgatg tggagatgac
     1801 gctggatgcc ttcaatgaat gcaatctggc caatgaggtc gtggtcgtca aggatgggca
     1861 ggaggcgctc gattatttgc agtgtcaggg gggctttgca tcacgattgc atggcaatcc
     1921 ggccgtgata ttgctcgatc tgaaactccc aaaaatagac ggtatgcaag tgctgcgccg
     1981 gatccgcgcc gatgaagagc tgaaactcat tccggtggtc atcatgacct cctcgcgcga
     2041 ggagcgcgat gtggcagagg gctatcggct cggtgtgaat gcctatgtgg ttaaaccggt
     2101 cgaattctcc gacttcgtcg atgcggtcaa ggggctgggc gtgttctggg cagtcctcaa
     2161 ccagccgccg ctgggctgcg caaacaggga tgtgtgatat gggtcttacc gatggaaaaa
     2221 ttcaagacag gctgcgtgtc ctgcatctgg aagataaccc gaacgatcgc gaattgatcc
     2281 gttcggaact cgaggccgaa tggccgcagg tcgaactgtc gggtgtcgct acccgtgcgg
     2341 ccttcttgca ggcgctggat gagttcaagc cggatctgat cctgtccgac tacaggctgc
     2401 ccgattacga tggccgctct gcgttaacgc atgtacgcca gcatcatccg gagattcccg
     2461 tgatcatggt cagcggcgcg ctgggtgaga ccgaagcggt tgaactggtc aagatgggcg
     2521 ccagagatta tgtgatgaag ggggagttgc aacgcctgcc cggtgcggta aagagtgcgt
     2581 tgtccgagga gtacggcata cgcgcccgca aggccgcaga aaaagcattg tggcaaagcg
     2641 aggccgatct ccgggcgtta gtggagagtt ccccgattgc catgatcgtg gatgtcggca
     2701 tgggggcgga tcaaaaagtc gtgatgatga accggcagtt catcaagctg ttcggttaca
     2761 cgatggcgga tgtgcccgat atacatcact ggtggcaact ggcttatccg gatgagcaat
     2821 atcgggaaaa gctcaaggcg gagtgggccg atcaggcgat ccggaagcac ggggccatcg
     2881 agcccaaaga tgtcacggtg acctgcaagg acggctcaag ccgctatgtc aggatttctc
     2941 tggcctcggc ggggaatcga aatatcgtca cgtttgaaga cttgactgca ggcaagcttc
     3001 tggaagatgc gcttcaggaa gcggttcacc ggaatcaggt gatgatggag actgtcaatg
     3061 acgctatcgt ctgtatcgat caggccggca tcatctatct gtggaacctc aaggctgagg
     3121 agatgttcgg ttattcggcg gctgaggcca tcggtcagga tttgcactca ttgatcacac
     3181 cggcgcgcta ttacgagtcg tcgactcacg cgctgggcaa gtttgcgcaa accggcaccg
     3241 gcccgatcat cggcgtcacg cgccagatcg atgccaagcg caaggacggc agcgagtttc
     3301 cggtggaact gtcggtttcc gcgatgagca ttcagggcac atggcatgcc accggcatca
     3361 tccgcgatat caccgaacgt aaacaggccg aatcccggat cgcgcggctg gttcagctct
     3421 atgcggcatt gagccagtgc aatcaggcta tcgtgcgcag caagaatcca acggacttgt
     3481 tcgagcagat ttgtcatgcc gccgtgactt tcggcggttt taaaatggcc tgggtcgggc
     3541 tgatcgaccc cggcacgggc atggtcaagc cggtcttcag ttttggtgag ggcgccgaag
     3601 agtatttgag aggcattcag atttcaatct cgatagagag cgagtttgga caggggccga
     3661 ccgggtgcgc gatacgcgaa aataaaccgt tctggtgcca ggatttcatg agtgatccgc
     3721 tcacatctcc ctggcatgat cgtgccatag ccttgggctg gaaaagctcc gccgctctgc
     3781 cgcttagccg ggagggggcg gttatcgggg cattcacgct ctacggtgac gaggtgaatc
     3841 aattcgatgc cgatgagcga aaactgctgg tggagatggc ggaggatgtc agctatgcgc
     3901 tggataattt caggcacgag accgaacgca aacagacaga agacgcgctg gtggaatcga
     3961 acaatctgct gaaagccgtc attcagacct cgcccatgcg cattttctgg aaggacaggg
     4021 agttgcgtta tctgggctgc aatgccgcct tcgcgcggga cgccggcgaa aaatctccgg
     4081 cagaaatagt cggcaaggat gattatcagc taggctggaa agcgcaggcg tcactgtatc
     4141 gtgctgatga caaccaggtc atggagtccg gcattccgat gctttcctat gacgagttgc
     4201 agagcacgcc ggacggcagg aacatttggt tgcgtacttc caaggtgccg ttacgcaatg
     4261 ccgccaatga aataatcggt gtgctcggta tctatgaaga cattaccgag cggaagttgg
     4321 tcgagctggc gctgcaacgc agcgaggccg acctcaaccg agctcaggcc gttgcacaag
     4381 tcggcagctg gaatctggat atcctcagta atcggcttga atggtcggcg gaagcctgtc
     4441 gcattttcgg catcccggga caagaggcgg tcgatctgga cactttcatt tccactattc
     4501 atcccgatga tcgcgaaatg gtgctcaagg catgggaggc ggccattgcg ggcgagccct
     4561 atgacatcga gcaccgcgtc gtggctgatg gcgtgacgcg ctgggtgagg gagcgcgccg
     4621 ttatcgaacg cgatgcggct gggcacgcgc tgttcggtgt cggcacgacg caggacatca
     4681 ctgagcgcag gttggcagag gaccagttgc gaaaactatc gctggcggtg gaacagagcc
     4741 ccaattcgat cgtgatcacc gacctcaatg ccaaccttga atacgtcaac gaggccttcc
     4801 tctggactac gggctatagc cgcgaagagg tgataggcaa gaatccgcgc atcctgcatt
     4861 ccgacaagac ccctcgcgag acctatgatg atatgtgggc gcatctggcg cgaggagata
     4921 gctggaaggg tgaattcacc aaccggcgca agaacggcgg tgaatatatc gaatccatat
     4981 tcgtctcccc agtgcgcgat agcgacggga ctgtgaccca ctatgtgggt atcaaggagg
     5041 atattaccga gcgcaaaaag gtggaaaaat ccctgcagga gagcgaggaa aaattccgcg
     5101 tcatgagcgc gtcggcacag gatggtatcg tcatgatcga caatgacggg aacatctcat
     5161 tctggaaccg ggcggccgaa aatatcttcg gctattcggc aagcgaagcg ttgggcaaga
     5221 atcttcacct gttgcttagt ccggtcaggt ttcatgaagc atccgacaaa ggctttgccc
     5281 agtttcagga cagcggaaaa ggtgcggtca tcggaaaaac gctggagctc atggcgctgc
     5341 ataacagcgg caaagaattt ccgcttgaac tttccctcag tgcggtcaat atctctggcc
     5401 tttggcaggc catcggtatc gtccgggata tctcggagcg taaggaaaat caggaaaata
     5461 tgcttgcaca gtactcccat gtcgcgggcg tgaatgccca gctgatggcg gcgaacaagc
     5521 agctggaaca gacccagaag cagttgctgc aatccgagaa aatggcggcc gtgggcctgt
     5581 tggcggccgg cgtggcgcac gagatcaaca atccggtcgg ctatgtgaac tcgaatctcg
     5641 gtacgctgga aaaatatctt gccgatatct ttgtcgacat cgataaatat gaagcggccg
     5701 aggccgtgat ggccggcgac aacctcacgc tggatgaggt gcgtcagttg aaggcaagga
     5761 tcgatctcgc ctatttgcgc gaggacatca aggcgttgct ggccgaatcg catcaggggc
     5821 tggagcgcgt gaagaaaatc gtgctcgacc tgaaggagtt ctcccactcg gatgcccagg
     5881 atcagtgggt gtgggcggat gtgcaccatg gattggacac cacgttgagt gtggtgtgga
     5941 atgagctgaa atacaaatgc gaagtggtca aggagtacgg cgatctgcct aaaatctatt
     6001 gcctgccctc gcaactgaat caggtattca tgaatctgct ggtcaatgcc gcgcaagcca
     6061 tcgaggtgcg cggtaagatc acgctgagaa ccgggctgga aggtgacaga atatgggtcg
     6121 aggtgagcga taccggtcat ggcatttcgc cggagcatat cccgaggctg ttcgatccgt
     6181 tcttcaccac caagccggtg gggcagggga ccggtctggg gctgtccgtg tcctacaata
     6241 ttgtggagaa gcaccacggt tcgtttgagg tgcatagcga aatcgggcgg ggaacgacct
     6301 tccgcgtctg gctgccggtg caacaatctg acaacaagga gaaagcatga cgcaagacac
     6361 ggcagcgccc gggaatgttc cggccaccct gttgttcatc gacgacgagg ccaatattct
     6421 ggctgcactg aaacgcctgt tccggccgct gggttacaac attctgactg cagaaagcgg
     6481 cgcggaaggg cttgccctgc tggagaagga gaacatcgat ctggtgatct ccgacatgcg
     6541 catgcctaac atgacggggg ccgaactcct ggagcaggca cgcaacaaat ggcccgaggt
     6601 cattcgcatc ctgctgactg gctattccga tctcagctcc acgatcgcgg cgatcaatcg
     6661 cggcgaaatc tatcgttata tctccaagcc ctgggacgat aacgacatcg tgctgatcgt
     6721 gcacgatgcg ctggagcgca agcagctgct ggccgagaag cagcgtctgg aggcgctgac
     6781 gcaacagcag aacgaggcat tgaagaccct gaatgccagc ctggaggaca aggttcagca
     6841 gcgcaccgca gagctgcgca ccgcgatgaa ttcgctggca cgggcgcatg atcggttgaa
     6901 gcaggattat ttctccacga ttcaggtgtt cgccaacttg atggaattgc gcaagggcac
     6961 gatggcaggc cattcccgcc gcatcgcgcg tttgtgtcat gacatcgcat tgaagatggg
     7021 gctgccggag accgatattc atgcgataga gacggcggcc ttgctacaca acatcggcaa
     7081 gatctcgctg ccggatcgac tgctcgacac cccctatctt gccctgccct atgccgaccg
     7141 caccgaattc gacaagcatc cgctgcgtgc ggcggcggcg ctgatggcgc tggagccgtt
     7201 agcgcaggta tccgaactga tccgccatca ccgcgaacat tacgatggcg tgggcaaccg
     7261 ctccaatgtg cgcggcaatg atattccgct gggtgcccgc atcctgctgg tcgccagcga
     7321 ttacgacgcg ctgcaggaag gtttgctcgg gcaggacaag ctgacgccga atcaggcgct
     7381 ggacacgatc gtcaaggggg gcggcatgcg ttacgacccg aatgtcgtcg atgtcttccg
     7441 cgaggtggtc ggtcaatccg tgcagcaggg gcgcgtcgag gcggagttcc aggtaagcag
     7501 cagtcagctg cacgagggca tgctgctgac gcgggatgtg ctatcccgcg acgggatgtt
     7561 gctgctgctc aaggacacca tgttgaacgc ggggcatctt cgcgagatca gggaattcga
     7621 atcgtcgacc ggcgagtcgc tgacgatttt tacccatctg atgtaggttc gcgtagtgaa
     7681 cagttatcgt atcctgctgc tcgatgacga tgacttcgtg ctcgccgcct tgcgccgcga
     7741 gttgctgaac aaaccgtacg tcgggcatga cagtctggaa atcgaggctt tcgtatcgcc
     7801 tgcggccgct ttggcgcgcg caagggagcc ggagggctat ttcgacctgg tgatatcgga
     7861 cattcgcatg ccggagatga gtggcatcga ttttctcaag gccttcggcg agattcaacc
     7921 cgatgccgcc cgtatcgtcc tgagcggcga caccgacatg ccggagctga tgcgggcgat
     7981 caacgaggca catatcgatt ttttcatcgc caagccgtgg catgagtatg acctgaagag
     8041 tgcgctatgg cagacgctgc ggcaatacga tctgcgctgc gagaaccgga tgctggccag
     8101 acaatatctt gagcacttcc agttgccgca ccagttccgg cgaaaatcct gttatcagtt
     8161 gatggtggtg gacgacgacc agaatgttct gcatgcgctg caacgcgagt tgggcgcggc
     8221 ccatgccgag gggcgcgcgt tcgggcagta ccagcttgag gtgcatccgt tcgagagcgt
     8281 caatgcggcg ttgcaggcgg cgcgcgaact gcagttcgat gtggtcatca gcgattacgc
     8341 catgcccgga atgaacgggg tcgagttctt gcatttgttc agggaggcgc agtcgaatgc
     8401 ggtgcgcatc ctgctcagcg gcaatgccga tatggaggtc ttcgtgaatg cggtcaatac
     8461 ggccggagtc agctacttca tcgccaagcc gtggcgcgac tatgagttgc gcggcgcgat
     8521 cgaccgggcg ctgacctacc gcgagctggt attggaaaat cgttttctcg ctgaaatgtt
     8581 gcgtttaaga ggtcaacact gaaaggaatc cagacgtgta ccgtatcctg attgtggacg
     8641 atgaacagaa tgtgctgaat gcgttacggc gcgaattgca gggcgaatat gcggtcgaga
     8701 cctttgccag cccggaagaa gccctggcgc acagcctgca tgcgcaattc gatctggtga
     8761 tcgccgatta ccagatgccg ggcatgaatg gcatccagtt cctcaggcag ttcggcaagt
     8821 tgcaaccgga tgcggcccgg ctggtgctga gcggcggaac cgatttcgcc gcgctgatcg
     8881 gttcggtcaa cgagacgcat atctaccgct tcatcgccaa gccatgggac aagaccgagc
     8941 tggccgtgac gctggcacag gcgttggcgc atcgcgccac gctgctggag aatcggcgtc
     9001 tcgccgaact ttgccgcaag gaaaggcaat ggcggcgagc tcaggatccg gacaagcttt
     9061 atcaggtgct ggtggttgac gacgagccca acgtgctcag cgccgtgaag cgagacttga
     9121 ccgtgcatga gcgcttcgat gacctgcatg ccgcaatgcg gcatacggat aacccgggag
     9181 cgccagttga acatcacgac ttccgcttca atgtgcatac ggccagctcc ccgctgcagg
     9241 cactggagca agccagacag gtgagcttcg acgtggtgat tgcggattac ctgatgccgg
     9301 aaatggacgg gctgcgtttt ctggaagcct tccggcagct gcagccggac accgcgcgca
     9361 tcctgttttc cggtcatgcc gaccgggagg tgctggtcaa ggcgatcaac ggctccgaga
     9421 tatacggcta catcgagaaa ccatggcgcg agtatgtgct gaagaacacc gtttcgcagg
     9481 cggtggccta tcgcaatcta ttgcgcgaaa gccgccacct cgccgagcag atggcgcctt
     9541 gaccgcacat cgtccggcac caatgaaaac ggcccctgat cggggccgtt tcgcatgttg
     9601 gcggaagcgg tgagattcga actcacgaag gactctcatc ctcgctagtt ttcaagacta
     9661 gtgccttcaa ccactcggcc acgcttcctg tttgtatctg ctgcagactc gggcggctgt
     9721 taaggccttc gtcgagaggc gcgcattctg aagcaagcgg cccgagctgt ccagtagggg
     9781 cacggcgtgc cgtgcccttt cgactgccgt gcccctacga ggttcgttcg cagtcggccc
     9841 agttgttcgg cgtctcgtac aggcgcacgc gctccagacg caggttgttg ccgtacaagt
     9901 cggtgtaggc ggcgttgagg atgtcgaagg cgacctgcgc cagatgctcg gcggtcggca
     9961 ccacatcgag caccacggtc ttgtgctccg gcagcgtggc gaggaagtcg cacaccttgc
    10021 ggtcgttgcg atacaccaga aacgcgtgat cccacgcatc caccagcttc tccttggcga
    10081 tgcgcttcac atcggagaag tccatcacca tgccctgctc ggactggccc tcggtcgtga
    10141 tgagctcgcc ggacaaggtg atctcgatcg catagcgatg cccgtgcatg tgtctgcact
    10201 ggctgttgtg gttggggatg cgatgtccgg catcgaattc caggcggcgg gtgatgagca
    10261 tgatggctga agtcggttga ttggcgcgga ttataccctg tcagattttg caggtcgcgg
    10321 cgcgtttcag gaattcgcgc cgtgcctgtt catcctgcat gttgccgtag cgcaacgcgc
    10381 tgtagcgcgc ggcgaggtcg cggatgctgt cggccagtgc ggggcgtgcg gcggcgactc
    10441 gggtcgcgta gtcctgcggg ccttcgtgag gcttgcgcgg caggccggat ttttcgagct
    10501 tgcggcacag cttgagccag gcggcctgga ccttgtccgg ctgcggctgg atcaggtggc
    10561 gcagcatgaa cagcgcgaat gccgcgatta ccaggccgag gcctaggctc atgttcagcg
    10621 cgagtttctg ccaggagatg tcttccatgc cgaggcgggt gaggaaggcg aactggcgtt
    10681 ccgagttgta gcccagtacc cacaggttcc actggttgga cagcgcgtcc cagttcagcc
    10741 tcagtttgct cagccattgc ggcgggttgc gcgccatgaa gggcagggcg gcgttgttcg
    10801 gcacggcggc ggacaggccg cgctcgatgc gagccgggga gatggatgcg gtcgggtcga
    10861 tgcgcaccca gccctgtccg gaaagccaca cctccgccca ggcgtgggcg tcggactggc
    10921 gcacgatgta gtagccgccc acgtcgttgt attcgccgcc ctgatagccg gtgaccacgc
    10981 gcgccgggat gtgcgcggcg cgcatcagga acacgaagct tccggcgtag tgttcgcaga
    11041 agccctgccg ggtctcgaac aggaatgcgt cgatgttgtt cagtcccggc agcgggggcg
    11101 gttccagcgt gtagccgaag ttttcgcggt tgaaatagtt cagtgccttg ccgactaccg
    11161 ccgcatcgtt ggcgccctcg gcacgccatt gttcggcgag ttgttgcgcg cgcgggttga
    11221 gcccggctgg caattgcagc gcgcgctgta tctgccgttc ggattcgcgc agattggcgt
    11281 gataggtcag atgcgagcgc gcttcgtagc gcagccgcgt cttcagcggc tcttgctgca
    11341 cgatctggaa gtcgtgggtg agcctgacgg gtacggagat gctgtcaggc agatcgagcg
    11401 cgaacagcca ggtcttgttg tgcggctcca gtgtcacgct gtaggcgatg ggctggccta
    11461 gctcggtgaa ttgcggcgcg agggtgcgtg cagccctgcc ttgcgtccag gtgattccgt
    11521 cgaagttcca cagcaccggg ccgcgccagt acatctggtc gcgacgcggc ggcgtgccgt
    11581 gatagacgac gcggaatgcg acctcgtccg acagcgacag gcggccgaag ctgcccggcg
    11641 acatcttgtc gtccagcccg ctgctggcaa aggcatcatt cggcatgccc cacagcggtc
    11701 cctgcacgcg cgggaacagc acgaacagca ccagtgtcag cgggatggcc tgcagcagca
    11761 gcgtcgtcgc gaccttcagg cgcggcccga gcggcatgga ttgtccgtgc agatgcaccc
    11821 aggtggtgac gatgaccagc agggtggcga gcagatacag cgcggtgggg atgctctgcg
    11881 agtagaagaa gttggtgatg atgatgaagc aggcgagata gaccagcacc gtcgcatcgc
    11941 gcgcgctgcg cagttccatg aacttcagcg tggtcagcag gatcagcagc gtgacgccgg
    12001 cttcgcgtcc gaacagcgtg tggaactcga tcaggatgcc gcccgcactg gcaagggtga
    12061 gggtcatcag cagccagcgc ggcggcagcg gattgccgct gcgggccaga taggcgcgcc
    12121 agatcagcag cgcggcacac agcgctgaga cccacggcgg caggtgctcc gcatgggggg
    12181 ctgcgaccat caggatagac agcagcaggc cgtagagcag ggacttgctc atggtttccc
    12241 tctcgcccgg cccctctccc acgagtggga gaggggagta actggacccc ctctcccgtt
    12301 ttcgggagag ggggtgggga gagggcgctc ctgttgcggt aactcgaaca atgcaatccg
    12361 gcgcaggcat tcgtcgcggt gcgactgtcc gtcggcgggc ggcagctcac cgttcggcag
    12421 acgcaggccg tagcgcaatc cctgcgtttc ggcatccagc acccagcgcg tcatatggga
    12481 taacttgccc tcgatgtcag tctccggggt ggcggcgtag tccagccaca gctcttcgcc
    12541 gatctgcatg gagaattgtt tgacctgcaa gccgcgttcg cgcgcatagg ctttccacgc
    12601 gatgcgcggc agcgcgtcgc cttcgacgta gccgcgcaac ccggcgaaat cgtcgtcgcc
    12661 ggtgacgctg cgtttgccgg ttccgtccgg tgcatccccc ggtggcagcg cagcctccgc
    12721 cagaggcttg gggtagacca gacagcgcgt atcgaaatgc agatacgacc aggcatgaaa
    12781 caggccgagc ggaaattcgg tgtacagcgt cagcctgccc ggagtcagcc agccgcgttg
    12841 tctggcggcc agggggagcg cgacttcgca atcctgttgt gcggggacgt cgaagccgac
    12901 gggctcgccg tcggcgccgc gcagcatcag ttgatagcgc gccagtttgc tcgggttgcg
    12961 gaagtgcaga gggaagcgcg ccgtctcgcc gccgaacacc gcgtcgacgc gtccggcgcg
    13021 ggcttccaga tgcgccagat tgcggaaggc gtgcagcatg gacatcaccg ccatcatggc
    13081 cagcaaaaag gtcagcacat aacccagact caggctgtag ttgatgtcgc cgagcagcat
    13141 cagcaccagc acgaaggcca gtccgatgcc ttgccgggtg ggtaggatga agatgcggcg
    13201 ctgggtgagg gtgacggtcc cgtgctcgat cttcgggcgg aacagccaga tgcggaattt
    13261 ctgcttgagt gcggtcagca cgggtggcgc aggactattt tccaaacagg cgggcaatca
    13321 ccgcgtcgcg tgtcatgccg tgatgcgttg ccacatcggc gagtattgcg gctagcgtgc
    13381 ctatccgcaa agggtcatgc aatgggacgg tgagatgatg ctctttgttc gggagtgagc
    13441 cggtcagtcg cacatggctg ccggattgtc gtgttggctt atagttcagt cgttccagtg
    13501 ccttgatgag ctgtgcgccg gagagatctc ggggcagttt cataccgcga ggacttcctc
    13561 tcgggtgaaa tgcagccgga tgattttggg ctcctttcct tcttcgaagt ggcaatgaac
    13621 ggcatcccga acttgccggt gcaggctttc cacgtcgtcc gcttcggtga agatcgattc
    13681 gcccagcgcg cgggcaatca agcctccttc tggcgcctct tctacaacga acataatttc
    13741 attcatggtc atcctttcga cgggtgaggc atgcattcta gcatcgccag aaattacggg
    13801 atcgcgaccg ccgcgatcag ttcgcgcacc agcgtatcgc tgtccgg
//
LOCUS       DASUIV010000270         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_999402, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000270 DASUIV010000000
VERSION     DASUIV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999402"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1994
                     /locus_tag="VFW59_09790"
     CDS             24..1994
                     /locus_tag="VFW59_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX5364545.1"
                     /translation="MSAVSETPQKITFRRYKDGDNKVRRLNDKIFQAGSSYKCPTYIQ
                     STPPCQGSCPAGEDIRGYLGIVRGTEKVPNGADGKPVMPWQEYAWRRLTEANPFPAVM
                     GRVCPAPCESGCNRNEVEDHVGINSVEHFLGEYAIANNLKFNKPAVASSGKKVAILGG
                     GPAGLSCAYQLTLKGHEVTIFDDHEFLGGMMRYGIPGFRTPRDVLDAEIQRILDLGVK
                     TRLNCHVGRDITLEQIRKEFDAVFLGMGAQAGRALPIADSEAANVVTATAFLKAFNDG
                     RLQHVGKRVVVVGGGDTSIDVATVARRLGHIAHAKPTDAELAIAGRMAQDVADISVKQ
                     GAEVTLTSVFNVDKMQANQHEIEQALAEGIHILGSLVPIGIVRGADGRATALRVARCE
                     AKFAGGKLEIKNIEGSEHDIEADLIVSAIGQAVDFTGLEEFNNGKGAVSTDRNYVVNG
                     QSGVFAGGDVIRPHLLTTAIGHGSIAADGIHNFMLGMDLEKRPKIDAHQFDLMRKMVE
                     KGLKIEESHEPLRGTCDSNIAVHNFDNRSDRYVIPHDKLFLGHFSYVPRNQRDIVTLD
                     KESALGNFEERLVALVQEKAVAEAKRCMSCGMCFECDNCVVYCPQTAVSRVKKSEATL
                     GRYVQTDYDKCIGCHICADVCPTGYIQMGLGE"
     gene            1991..2362
                     /locus_tag="VFW59_09795"
     CDS             1991..2362
                     /locus_tag="VFW59_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364546.1"
                     /translation="MMKLLTSLLLCLCVSAVWAGEGSTPKPDIGRGGQCVKDPQWMRK
                     NHMHMLKHERDETVRQGIRDDKHSFKACIECHASTKDDSVIAREDSFCVSCHRYEAVK
                     IDCFECHSGTRKSAWLQRNAK"
     gene            2359..3075
                     /locus_tag="VFW59_09800"
     CDS             2359..3075
                     /locus_tag="VFW59_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5364547.1"
                     /translation="MNDIDSQRRSFLKTALGAAAVTIAPGMMLYRVAQANTEGASSRV
                     RWGMLIDTAQCKEGCTDCVSACNRENGLSGGKAATDSQWIRKIEIKDISTGRQTSLPM
                     MCQHCEHPPCVDVCPTAASFKRADGIVLVDRHRCIGCRYCMMACPYKARSFVHEELHD
                     QNPDVPRGKGTVESCTLCVHRIDQGGKPACAEACANKAILFGDLNDPSSEIAQKIRSV
                     ASIQVRAELGLNTGIRYQGF"
     gene            3076..4257
                     /gene="nrfD"
                     /locus_tag="VFW59_09805"
     CDS             3076..4257
                     /gene="nrfD"
                     /locus_tag="VFW59_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5364548.1"
                     /translation="MSSYPPMYFKNRLYWLLLAAGGLVALAGLYAVHMMESHGHIITG
                     MNNQVVWGIPHVFAIFLIVAASGVLNVASIGSVFGKAIYKARAPLASLLSIAMLSGGL
                     TVLMLDLGRPDRIIVAATNYNVTSVFAWNVVLYSGMFSLVALYLWTMMERRMNPWSKP
                     AGLAVFVWRFVLTTGTGLIFAFLTARQAYGSALLPPMFIVLSFAWGLAVFHVVQTVIY
                     AWNEKTLDPAILQRKKNLLGVFVIGSLYMVAVYHMTNLYFAHQTGFERFILRDGGVYP
                     ALFWWGYVVVGNVLPLLLIFLPNLGKTRCVMAASSLVVLGAFALLYVFIIGGQAYPLN
                     IFPGYEVTSSFGDGQIASYQPSLYEILLGFAGLAIAFIITTVSVRVLNFMPQDKAYIA
                     D"
BASE COUNT          898 a         1291 c         1417 g          914 t
ORIGIN      
        1 cgaaataacg aggagaagca aaaatgtcag cagtgagcga aaccccgcag aagattacgt
       61 tccgtcgtta caaggatggc gacaataaag tccgccgtct gaacgacaag attttccagg
      121 ccggctcctc gtacaagtgc ccgacttata tccagtccac accgccatgc cagggcagct
      181 gccctgcggg cgaagatatt cgcggctact tgggcatcgt gcgcggcacc gaaaaagtgc
      241 cgaacggcgc cgatggcaag ccggttatgc cgtggcagga atatgcatgg cgccgcctga
      301 ccgaagccaa ccccttcccg gcagtgatgg gccgtgtgtg cccggcgccg tgcgaaagcg
      361 gttgcaaccg caacgaagtg gaagaccacg tcggcatcaa ctcggtcgag catttcctcg
      421 gcgaatatgc gatcgccaac aatctgaaat tcaacaagcc tgcggtcgct tcttccggca
      481 agaaggtcgc gatccttggc ggcggcccgg ccggtctgtc ctgcgcctat cagctgacgc
      541 tgaagggtca cgaagtcacc attttcgacg accatgaatt tctcggcggc atgatgcgct
      601 acggcattcc cggtttccgt actccgcgcg atgtgctgga tgcggagatt cagcgcattc
      661 tcgatctggg tgtgaagacg cgcctgaatt gtcatgttgg ccgtgacatc acgctggagc
      721 agatccgcaa ggaattcgat gcggtattcc tcggcatggg tgcccaggcc ggccgtgcgc
      781 tgccgatcgc cgattcggaa gccgctaacg tggtgaccgc gaccgccttc ctgaaggcgt
      841 tcaacgacgg tcgtctgcag catgtcggca agcgcgtggt ggtggtcggc ggcggcgata
      901 cctccatcga cgtggcgacc gttgcgcgcc gcctcggcca tatcgcgcat gccaaaccga
      961 ccgacgcgga actggcgatc gccggacgca tggcgcagga tgtcgccgat atttccgtca
     1021 agcagggggc ggaagtcacg ctgacctcgg tgttcaacgt cgacaagatg caggccaacc
     1081 agcacgagat agagcaggcg ctggccgaag gcatccatat tctcggcagt ctggtgccga
     1141 tcggcatcgt gcgcggcgcg gacggccgcg ccacggcatt gcgcgttgcc cgttgcgaag
     1201 cgaagttcgc gggcggcaag ctggagatca agaacatcga gggcagcgag catgacatcg
     1261 aggccgacct gatcgtttcc gcgatcggtc aggctgtgga cttcaccggt ctggaagaat
     1321 tcaacaacgg caagggcgcg gtctcgaccg accgcaacta tgtggtgaac ggccagtccg
     1381 gagtgttcgc cggcggcgac gtgatccgtc cgcatctgct gaccaccgcg atcggtcacg
     1441 gctccatcgc ggccgacggt attcataact tcatgctggg catggatctg gagaagcgcc
     1501 ccaagatcga cgcgcaccag ttcgacctga tgcgcaagat ggtggagaag ggcctcaaga
     1561 tcgaggaatc gcatgagccg ctgcgcggca cctgcgactc caatatcgcg gtgcacaatt
     1621 tcgacaaccg ttccgaccgt tacgtcatcc cgcatgacaa actgttcctg gggcatttct
     1681 cctacgtgcc gcgcaaccag cgcgacatcg tgacgctgga caaggagagc gcgctgggca
     1741 acttcgagga gcgtctggtc gcgctggttc aggagaaagc ggtggccgag gccaagcgct
     1801 gcatgagctg cggtatgtgc ttcgagtgcg ataactgcgt ggtgtactgc ccgcagaccg
     1861 cagtgtcccg cgtcaagaaa tccgaggcga cactcggacg ctatgttcag accgactacg
     1921 acaagtgcat cggctgccat atctgtgccg acgtgtgccc gacgggttat atccagatgg
     1981 gtctgggcga atgatgaagc tgctcacatc gctgttgctc tgcctctgcg ttagcgcagt
     2041 ttgggcagga gaggggagta cgcccaagcc ggatattggc cgtggcggac agtgtgtgaa
     2101 agacccgcag tggatgcgca agaaccatat gcatatgctc aagcacgagc gcgatgaaac
     2161 tgtgcgtcag ggcatccgcg atgacaagca cagcttcaag gcttgcatcg agtgtcacgc
     2221 cagtaccaaa gacgacagtg tgatcgcacg cgaggacagc ttctgcgtgt cgtgccatcg
     2281 ctatgaagcg gtcaagatcg actgtttcga gtgtcactcc ggtacacgca agagcgcatg
     2341 gttgcagagg aacgcgaaat gaacgacatc gacagccaac gcagaagttt tctgaaaacg
     2401 gcactgggcg cggctgcggt cacgatcgcg ccgggcatga tgctttatcg agtggcgcag
     2461 gccaacaccg aaggcgcatc gagccgggtg cgctggggga tgctgatcga cacggcgcag
     2521 tgcaaggaag gctgcaccga ttgcgtgagc gcatgcaaca gggagaacgg attgtccggc
     2581 ggcaaggcgg cgaccgactc gcagtggata cgcaagatcg agatcaagga catcagcacc
     2641 gggcgtcaga cctcgctgcc gatgatgtgc cagcattgcg agcatccgcc gtgcgtggat
     2701 gtgtgcccga cggccgcgtc gttcaagcgt gccgacggca tcgtgctggt ggatcgccac
     2761 cgttgcatcg gctgccgcta ttgcatgatg gcctgtccgt acaaggcgcg ttccttcgtg
     2821 catgaagaac tgcacgacca gaatccggat gtgccgcgcg gcaaaggcac ggtggaaagc
     2881 tgcacgctgt gcgtgcaccg catcgatcag ggcgggaaac cggcttgtgc cgaagcctgc
     2941 gcgaacaaag cgatcctgtt cggcgacctg aacgatccgt ccagcgagat cgcgcaaaag
     3001 attcgttcgg tggcctccat acaggtgcgc gccgagcttg ggctcaacac cggcattcgt
     3061 taccaggggt tctagatgag cagctatcct ccgatgtatt tcaagaaccg tctgtactgg
     3121 ctgttgctgg ccgccggcgg attggtcgcg ttggccgggt tgtatgcggt gcacatgatg
     3181 gagtcgcacg gtcacatcat caccggcatg aacaatcagg tggtgtgggg tattccccat
     3241 gtgttcgcga tctttctgat cgtagccgcg tcgggcgtgc tgaacgtggc gtccatcggt
     3301 tcggtgttcg gcaaggcgat ctacaaggcg cgcgcgccgc tggcgagttt gttgtccatc
     3361 gccatgctgt ctggcggtct gacggtgctg atgctggatc tgggcaggcc ggaccgcatt
     3421 atcgtcgccg cgaccaatta caacgtgact tcggtattcg cctggaacgt ggtgttgtat
     3481 tccggcatgt tctcgctggt ggcgctctac ctgtggacca tgatggaacg ccgcatgaat
     3541 ccctggtcga aaccggcagg tctggcggtg ttcgtatggc gcttcgtgct gaccaccggc
     3601 accggcctga tcttcgcctt cctgaccgcg cgtcaggctt acggctccgc gctgttgccg
     3661 ccgatgttca tcgtgctgtc gttcgcctgg ggtctggcgg tgttccatgt ggtgcagacc
     3721 gtgatctacg cttggaacga gaagacactg gatcccgcga ttctgcagcg caagaagaac
     3781 ctgctcggcg tgttcgtcat cggttcgctg tacatggtcg cggtctacca catgaccaac
     3841 ctgtatttcg cgcaccagac cggtttcgag cgtttcatcc tgcgcgatgg cggcgtctat
     3901 ccggcgctgt tctggtgggg ttacgtggtg gtcggcaatg tgctgccgct gctgttgatc
     3961 ttcctgccga atctgggcaa gaccaggtgc gtgatggcag catcctcgct ggtggtgctg
     4021 ggcgccttcg ccctgctgta tgtgttcatc atcggcgggc aggcgtatcc gctcaatatc
     4081 ttcccgggct atgaagtcac cagcagcttc ggcgacgggc agatcgcgag ctaccagcct
     4141 tctctgtacg agatactgct cggcttcgcc ggccttgcga ttgccttcat catcaccacg
     4201 gtcagcgtgc gggtgctgaa tttcatgccg caggacaaag cgtatatcgc agattaaaca
     4261 aacaaaacca attgtccgca gattacacag attacgcaag attaaaaccg aaaatccatg
     4321 aagagtggtt gttgcccgca ggttcaaaaa tctgcgtgaa tctgcgtaat ctgcggataa
     4381 agattttgct tttagctgtt tgtttcaaaa ggctgccatg aatcgtttcc ttatttcggc
     4441 tgcgcacaag tcttccggca agacgatggt cagtatcggc ctgtgcgccg cgctcgcggc
     4501 acgcggccat gccgtgcagc
//
LOCUS       DASUIV010000271         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_628288, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000271 DASUIV010000000
VERSION     DASUIV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628288"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..965)
                     /gene="aroE"
                     /locus_tag="VFW59_09810"
     CDS             complement(162..965)
                     /gene="aroE"
                     /locus_tag="VFW59_09810"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5364549.1"
                     /translation="MTDKYAVIGHPIAHSKSPLIHRMFAEQTGQDISYEAIEAPLDGF
                     AATIERLRAEGYKGCNVTVPFKFEAYELATELSERAQTAQAVNTLKFEGEAIFGDNTD
                     GAGLVRDIEQNLGVSLRDKRVLLMGAGGAAYGVVLPLLTSGACITVVNRTADKARQLA
                     AVFAEQSGIAGCGYDELEDLHDIVVNATSAGLSDSTIPLPDTIFAPGALAYDMMYGRE
                     TPFMTFAREHGAQVADGLGMLVEQAAEAFFIWRGVRPDTRPVLEKFRTT"
     gene            complement(970..1821)
                     /locus_tag="VFW59_09815"
     CDS             complement(970..1821)
                     /locus_tag="VFW59_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX5364550.1"
                     /translation="MIATLTARMTGAMTLSIALHAFALFGVALVLPNSSNTASIMPPL
                     QVVLVNSKSKSRPVKADALAQANLDGGGNTAEDRQAKTSMPTISDDQQFTPEQVAKRV
                     AELEHESKRMLSRLKSDYTVAQPTPQKERNNDSHSGDDLVQRSLEIARLEAQISKNAD
                     AYQKLPRRKFVGARTQEFRFAQYIEDWRVKVERIGNLNYPEEARRKQIYGKLQLSVSI
                     RADGTLENIEVSRSSGQRILDAAAMRIVKLAAPYSPLPPEITKDTDILTITRTWTFTP
                     SDRLEGE"
     gene            complement(1818..>2843)
                     /locus_tag="VFW59_09820"
     CDS             complement(1818..>2843)
                     /locus_tag="VFW59_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5364551.1"
                     /translation="GMPRDSEGDKVAAERLSTVYMPGQKITMLPDSVVQAFTLCADRV
                     CPALSMYNEVDAETLAILSHESRVERIHVAANLRHDTLEPLFNEETLAAGKLDYPYAP
                     ELALLWKLAQKLEATRGKSSDNSTQQLDYNFNIENDRVSITTRRRGSPIDKVVSELMI
                     LVNSEWGRHIAEHGFVAIYRTQQGGKVKMSTVAAPHQGLGVAQYMWSSSPLRRYVDLL
                     NQRQIIAMLNDAEPVYPKNDTALFAAMRDFDTMYSIYNEFQRHMERYWCLRWLQQEQG
                     EDELVVTATVLRENLVKLVDIPLVFRASSLPELPANTRVKLAITGIDLLDVSVETRFI
                     ATVEEAA"
BASE COUNT          472 a          835 c          914 g          622 t
ORIGIN      
        1 attcttgtcc tgcatcagct ccagacggtc ttccatgaaa gaactgctgg aaggatcgaa
       61 cttcacccac catacgatgt gggcaaagat gtaaacctga tagagaagca ccagcgcgaa
      121 cagcgccagc aggatgcgcc atgtccagca tttgaaggtt ttcatgtagt gcggaatttt
      181 tccagaacgg ggcgtgtgtc ggggcgtacg ccgcgccaga tgaagaaggc ctcggcggcc
      241 tgttcgacca gcatgccgag gccgtcggca acctgtgcgc catgttcgcg tgcgaaggtc
      301 atgaacggcg tctcgcgccc gtacatcatg tcgtaggcca gtgcgccggg ggcgaagatg
      361 gtgtccggca aggggatagt gctgtcggaa aggccggcgg aggtggcgtt gacgacgatg
      421 tcgtgcaggt cttccagttc gtcgtagccg cagccggcaa tcccactctg ttcagcgaat
      481 acggctgcaa gttgccgcgc cttgtctgca gtgcgattga ccacagtgat gcaggctccg
      541 ctggtaagca gaggcagcac gactccgtaa gcagcccctc cggcccccat cagcagcacg
      601 cgcttgtccc gcaatgaaac cccgaggttt tgttcgatgt cgcgcacaag ccctgcgcca
      661 tcggtgttgt cgccaaagat ggcctcgcct tcgaacttga gtgtattcac cgcctgtgcg
      721 gtttgcgcgc gttcgcttag ttcagtggct aattcgtatg cctcgaattt gaacggcacc
      781 gtcacattgc aacccttgta gccctctgcg cgcaggcgtt ctatcgttgc ggcgaagccg
      841 tccagcggcg cctcgatggc ctcgtaactg atgtcctgcc cggtctgttc ggcaaacatg
      901 cggtggatca gcggcgattt gctgtgcgcg atgggatggc cgatcacggc gtatttgtcg
      961 gtcatgctgt tattcgcctt ccagccggtc ggacggggtg aacgtccagg tgcgggtgat
     1021 ggtcaggata tcggtgtcct tggtgatctc cggcggcagc ggggagtagg gggcggcgag
     1081 cttgacgatg cgcatcgccg ccgcatctag aatgcgctgg ccggagctgc ggctgacttc
     1141 aatgttttcc agcgtgccgt ccgcgcggat cgagacgctg agttgcagct tgccgtagat
     1201 ctgtttgcgg cgcgcctctt ccgggtagtt caggttgccg atgcgctcga ccttgacgcg
     1261 ccagtcttcg atgtattgcg cgaagcggaa ttcctgagtg cgtgcgccga cgaacttgcg
     1321 gcgcggcagc ttctggtagg catccgcatt tttgctgatc tgcgcctcca gccgggcgat
     1381 ctccagactg cgctgcacca gatcgtcgcc gctgtggctg tcgttgttgc gttccttctg
     1441 cggcgtcggt tgcgcgacgg tgtaatcgct cttcagtctg gacagcatgc gcttggactc
     1501 gtgctccagc tcggcgacgc gtttggcgac ctgctccggg gtgaactgct ggtcgtcgct
     1561 gatggtgggc atcgaggtct tggcctgacg gtcttcggcg gtgttgccgc cgccgtccag
     1621 attggcctgt gccagtgcat cggccttgac cggtctggat ttcgacttgc tgttgaccag
     1681 caccacctgc agcggcggca tgatgctggc agtgttgctg ctgttcggca gcaccagtgc
     1741 tacgccgaac agcgcgaagg catgcagcgc gatcgacagg gtcatcgcgc cggtcatgcg
     1801 tgcggtcaat gttgcgatca agcggcctcc tcaaccgtgg cgataaagcg tgtttcgaca
     1861 ctgacatcca gcagatcgat gccggtgatg gcaagtttca cgcgggtgtt ggcgggcagc
     1921 tcgggcagcg acgaggcgcg gaacaccagc gggatgtcga cgagcttcac cagattctcg
     1981 cgcagcacag tggccgtaac gacaagctca tcctcgccct gttcctgctg caaccagcgc
     2041 aaacaccagt agcgttccat gtgacgctgg aactcgttat agatgctgta catggtgtcg
     2101 aagtcgcgca tcgcggcaaa cagcgcggtg tcgttcttcg gatagaccgg ttcggcatcg
     2161 ttcagcatcg cgatgatctg gcgctggttc agcagatcca catagcggcg cagcggcgag
     2221 ctcgaccaca tgtattgcgc cacgcccaag ccctgatgcg gtgcggcgac tgtgctcatc
     2281 ttcaccttgc cgccctgctg ggtgcggtag atggcgacga aaccgtgttc ggcgatgtgc
     2341 ctgccccact cgctgttgac cagaatcatc agctcggaca ccaccttgtc gatgggcgag
     2401 ccgcgtcgcc gcgtcgtgat gctgacgcga tcgttttcga tgttgaagtt gtaatcgagc
     2461 tgctgggtgc tgttgtcgga tgacttgccg cgcgtggctt ccagcttctg cgccagtttc
     2521 cagagcagtg ccagttccgg cgcgtagggg taatcgagtt tgccagcggc cagcgtctct
     2581 tcgttgaaca gcggctccag cgtgtcgtgg cgcagattgg cggcgacatg gatgcgctcg
     2641 acgcggcttt cgtggctcaa gatcgccagc gtctcggcgt ccacctcgtt gtacatcgac
     2701 agtgccgggc agacgcgatc cgcgcacagc gtgaaggcct gcactacgct gtccggcagc
     2761 atggtgatct tctgtccggg catgtacacc gtggacaggc gttcggcggc caccttgtcg
     2821 ccctcggaat cgcgcggcat gcc
//
LOCUS       DASUIV010000272         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_70767, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000272 DASUIV010000000
VERSION     DASUIV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70767"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFW59_09825"
     CDS             complement(<1..549)
                     /locus_tag="VFW59_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5364552.1"
                     /translation="MDSPKLATELRQAALFWLAECDAVKKAEGVRGLAQAWAAGEVEL
                     DGEAVLTATRPSPGLPPKPELVSPLSVKRRAMSTPEGRAAMIHALVHIEFHAINLALD
                     AIWRFAGMPRAYYADWLQVADEEALHFTLLAEHLQGLGYVYGDFPGHGSLWEMASKTR
                     HDVLARMALVPRTMEARGLDVVP"
     gene            complement(556..2553)
                     /locus_tag="VFW59_09830"
     CDS             complement(556..2553)
                     /locus_tag="VFW59_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5364553.1"
                     /translation="MLSKLNAVQRDAATYLDGPLLVLAGAGSGKTRVITHKIAYLVEE
                     CGYAARNIAAITFTNKAANEMRERVGSLLQGGSGKGLVVSTFHSLGMNILRKEAALLG
                     YKPQFSIFDSSDSWKILSELTNSGDKQEIRDLQTQISNWKSAFVSPGQAGALAENDEA
                     QVHARIYARYQETLRAYQAVDFDDLIHLPVVLFNEHPEALARWQQRLRYLLIDEYQDT
                     NDCQYQLTRLLAGKRAAFTAVGDDDQAIYAWRGASIENLHNLRNDYANLRVIKLEQNY
                     RSSQRILKVANHLINHNTKVFEKKLWSDHGVGDPIRIYAARDDEHEAESVVMKLMAHK
                     FEHRTRFADYAILYRSNYLSRAFEEQLRAQRVPYTVSGGTSFFDKAEIKDITAYLRLI
                     ANPDDDPAFIRAITTPKRGIGNTTLEKLGSYAGSRQVSLFEAAFETHMANELPQRQYE
                     DLMLFCNFINRIQDRADKDPCGELLDELLRAINYETWLYDSHESRQAEGKWKNVEDFI
                     GWLKRKAEADEKSLIAMVQTIALINMLDGKDKEPDAVTLSTLHAAKGLEFPHVFIVGA
                     EEEILPFRDSDEKGIEEERRLMYVGVTRAERSLQISYCNRRKRGKDWFACEPSRFLDE
                     MPESELQYAGGHAEAAPTVTKDEGMDKLARLKAMLSKPAEG"
BASE COUNT          496 a          899 c          816 g          554 t
ORIGIN      
        1 aggcacgacg tccagcccgc gcgcctccat cgtccggggc accagcgcca tgcgcgccag
       61 cacatcatgc cgcgtcttgg atgccatttc ccacaggctg ccgtgcccgg gaaaatcgcc
      121 gtagacatac cccaacccct gcaaatgctc ggccagcaag gtgaagtgca acgcctcctc
      181 atcggccact tgcagccagt cggcgtaata ggcgcgcggc atgccggcaa agcgccagat
      241 cgcatccagc gccagattga tggcatgaaa ctcgatatgc accagcgcat ggatcattgc
      301 ggcgcgccct tcgggggtgc tcatcgcgcg gcgcttgacc gaaagcggcg acaccagctc
      361 aggcttgggc ggcagaccgg gactgggccg agtggcggtc aagacagcct cgccgtccag
      421 ctcgacctcc cccgctgccc aggcttgcgc cagaccgcgc accccttcgg cttttttaac
      481 ggcgtcgcat tccgccagcc agaacagcgc ggcctgcctt aattccgtag caagtttcgg
      541 cgagtccatg aacgatcaac cctcggcggg cttgctcagc atcgccttca gcctcgccag
      601 cttgtccatg ccctcgtcct tggtcacggt cggcgcggct tccgcatgac cgcccgcata
      661 ctgcaactcg ctttccggca tttcatcgag aaagcggctc ggttcgcagg cgaaccaatc
      721 cttgccgcgc ttgcggcggt tgcaatagct gatctgcagg ctgcgctcgg cacgggtaac
      781 gccgacgtac atcaggcggc gctcttcctc gatgcctttc tcgtcgctgt cgcggaacgg
      841 caatatctcc tcctccgcgc cgacgatgaa cacatgcggg aattccagcc ccttggcggc
      901 atgcagcgtg gacagcgtca ccgcatcggg ctctttgtcc ttgccatcga gcatattgat
      961 caatgcgatg gtctgcacca tcgcgatcag cgacttctcg tccgcctctg ccttgcgctt
     1021 gagccagccg atgaaatcct cgacgttttt ccacttaccc tcggcctgcc gcgactcgtg
     1081 cgagtcatac agccaggtct cgtagttgat cgcgcgcagc aactcgtcca gcaactcgcc
     1141 gcagggatct ttgtcggcac gatcctgaat gcggttgatg aagttgcaga acagcatcag
     1201 atcctcgtac tgccgctgcg gcaattcgtt cgccatatgc gtctcgaatg ccgcctcgaa
     1261 caggctgacc tgacgcgatc cggcgtagct gccgagcttc tccagcgtgg tgttgccgat
     1321 gccgcgcttc ggggtcgtga tcgcgcgaat gaaggccgga tcgtcgtccg gattcgcgat
     1381 caggcgcagg taagcggtga tgtccttgat ctccgccttg tcgaagaacg aggtgccgcc
     1441 gctgacggtg taaggcacgc gctgggcgcg cagctgctcc tcgaaggcgc gcgacagata
     1501 gttgctgcga tacaggatgg cgtagtcggc gaagcgcgtg cggtgttcga acttgtgcgc
     1561 catcagcttc atcaccacgc tctcggcctc gtgctcgtcg tcgcgcgccg cgtagatgcg
     1621 gatcggatcg cccacgccgt ggtcgctcca cagcttcttc tcgaacacct tggtgttgtg
     1681 gttgatcagg tggttcgcca ccttgaggat gcgctgcgat gagcggtagt tctgctccag
     1741 cttgatcacg cgcaggttgg cgtagtcgtt gcgcaggtta tgcaggttct cgatgctcgc
     1801 gccgcgccag gcatagatcg cctgatcgtc gtcgcccacc gccgtgaacg ccgcgcgctt
     1861 gcccgccagc aaccgggtca actggtactg gcaatcgttg gtgtcctgat attcgtcgat
     1921 cagcaggtag cgcaagcgct gctgccagcg cgccaaggcc tcggggtgtt cgttgaacag
     1981 caccaccggc agatggatca ggtcgtcgaa gtccaccgcc tgataggcgc gcagcgtctc
     2041 ctgatagcgg gcatagatac gcgcatgcac ctgcgcctca tcgttctccg ccagagcccc
     2101 ggcctgtccc ggcgagacga aggccgactt ccagttcgaa atctgcgtct gcaggtcgcg
     2161 tatctcctgc ttgtcgccgg agttggtcag ctcgctgagt atcttccagc tgtcgctgga
     2221 atcgaagatc gagaactgcg gcttgtagcc taacagcgcc gcctccttgc gcaggatgtt
     2281 catgcccagc gaatgaaagg tactcaccac cagccccttg ccgctgccgc cttgcagcaa
     2341 gctgccgacg cgctcgcgca tctcgttggc cgccttgttg gtaaaggtga tcgccgcgat
     2401 attgcgcgcc gcatagccgc actcctccac cagataggcg atcttgtggg taatcacgcg
     2461 cgtcttgccg ctgcccgccc ccgccagcac caacaagggg ccgtcgagat aggtcgccgc
     2521 atcgcgctga actgcattga gcttgcttaa catgtgggga ctgctgaagt gttgccggaa
     2581 aggttggcat gataccaaag caagcgcatc aaaatatccg aggtaaaggc taattttttg
     2641 accggggccg ccgatagtcc aatcaagggc ggcgcattgg cactgccacc gataacagga
     2701 ggacatcatg agcgtactga cgatggacat gagcagctac gaggtcgaac gcagcaaggc
     2761 tcccg
//
LOCUS       DASUIV010000273         6080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_628607, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000273 DASUIV010000000
VERSION     DASUIV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628607"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFW59_09835"
     CDS             complement(<1..800)
                     /locus_tag="VFW59_09835"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5364554.1"
                     /translation="MSDIKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEE
                     LEPARQKALKFGIKPESIFIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIA
                     KRLIEIATKTGAQAISHGATGKGNDQVRFELGAYALNPNIKIIAPWREWDLLSREKLM
                     AYAEQHGIPVDMKHKQGGSPYSMDANLLHISYEGRHLEDPAAEAEDSMWRWSVSPEKA
                     PDAAEYLEIEFERGDIVALNGTKMTPAQVLAKLNELGGKHGIGRLDLV"
     gene            complement(816..1157)
                     /locus_tag="VFW59_09840"
     CDS             complement(816..1157)
                     /locus_tag="VFW59_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364555.1"
                     /translation="MSDTALQTPSAEQHGESRLADHILPSSATMIGVCMTVISLAHLI
                     PQRSISRHVNEMLAIDSLLFLGSAMLSYFSIRHPQRAEKFERMADVIFLFAISLMVMV
                     GFIVSFELFID"
     gene            complement(1154..2092)
                     /gene="argF"
                     /locus_tag="VFW59_09845"
     CDS             complement(1154..2092)
                     /gene="argF"
                     /locus_tag="VFW59_09845"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5364556.1"
                     /translation="MPTIPSSAVKHFLQFKDLSREELEYLFDRTRVIKQRFKSYQQYW
                     PLQDRTLVMIFEKASTRTRLSFEAGMQQLGGSAIYLNTRDSQLGRGEPVEDAGQVISR
                     MSDIVMIRTFEQSIIERFAAHSRVPVINGLTNEYHPCQILADIYTYIEQRGSIQGKTV
                     AWIGDSNNMCNTWLQAAEILDFNVHVSTPPGYEVEPERAGLYGNEHYEEFTDPMEAAR
                     GADLVTTDVWTSMGFEAENEERMKDFADWQVDADMMRVAAKDALFMHCLPAHRGEEVA
                     AEVIDGPQSVVWDEAENRLHVQKALMEYLLLGKVST"
     gene            complement(2106..3278)
                     /locus_tag="VFW59_09850"
     CDS             complement(2106..3278)
                     /locus_tag="VFW59_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5364557.1"
                     /translation="MSHVMNTYARLPVAFARGEGSWLWDNEGRRYLDGLSGIAVNTLG
                     HAHPRFTAALNEQIGKLMHCSNVYQVGEQEQLADKLSALSGMQEAFFCNSGCEANEAA
                     IKLARMYGHNKGVEMPSIIVMEKAFHGRTLATLSATGNRKVQAGFEPLVKGFVRVPYN
                     DIPAIEQVATHNPDVVAVLVEPIQGEGGIRTLDIHYLEQLRKICDERGWLLMLDEVQS
                     GIGRTGKWFAHQHSGILPDVMTLAKGLGSGMPIGACLAAGKAAGTFKPGNHGSTFGGN
                     PLACVAGLTTLNILEQDQLLAHAETLGLFIRQGFASALQGVDGIKDIRGQGLMIGLEL
                     DKPCGELVKLALARGLLINVTADNVIRLLPPLTMSQDEAQLLIDGLCPLIKEFLQS"
     gene            complement(3470..3733)
                     /gene="rpsT"
                     /locus_tag="VFW59_09855"
     CDS             complement(3470..3733)
                     /gene="rpsT"
                     /locus_tag="VFW59_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5364558.1"
                     /translation="MANSAQARKRARQAVKQNAHNSSLRSELRTAIKKVIKAVEAGDK
                     AAAQAVYQASVSTVDSIADKKIIHKNKAARHKSRLSAAIKAMA"
     gene            3960..4298
                     /locus_tag="VFW59_09860"
     CDS             3960..4298
                     /locus_tag="VFW59_09860"
                     /inference="COORDINATES: protein motif:HMM:NF023534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEX5364559.1"
                     /translation="MFREQQEWVILGVTEAGEVFDVAGWAERLCGMLANQGRDNRVNY
                     SDYLTPAHIEGLPAVVMLSRLEQDDPASFAVVRQFVAENRLKTRSGRSGSATGKHPVL
                     LQERRSYIKG"
     gene            complement(4324..4962)
                     /locus_tag="VFW59_09865"
     CDS             complement(4324..4962)
                     /locus_tag="VFW59_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364560.1"
                     /translation="MLSALTGCASMSGARTQTIAVQTVQDHKEVTAAGCILANGVGRW
                     FITTPGQIIINKNIDDLTVECSKEDVGSGSETIASKANGGMWGNVLLGGPIGYVVDRK
                     SGAGYDYPQTIVVELKKIEKAEERDGTTETKTDDGWSPPAAIEQPAAGEQTAAPEVRQ
                     DPPTQAPPAEERHTQAPPAKNKLVEQKTAPANDWQPPPTEAAGTATPYISNY"
     gene            5118..>6080
                     /gene="murJ"
                     /locus_tag="VFW59_09870"
     CDS             5118..>6080
                     /gene="murJ"
                     /locus_tag="VFW59_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX5364561.1"
                     /translation="MNLLKALAAISSLTLVSRILAFARDVLIARIFGAGGATDAFFVA
                     FKLPNLLRRLFAEGAFSQAFVPIFGEYKNRRGQEETKLLVDHVTTMLALILFVVTLIG
                     IVAAPILVYISAPGFVKDAAKFDLTVQLLRITSPYIFFISLVAVAASILNTYNKFWVP
                     AFAPILLNLCFIGGALWLAPYCNPPILALAWAVFIAGIVQLAFQIPFLKQIGMLPTLR
                     FNLKDAGMWRVIKLMGPAAFGVSIAQISLIVNTIIASFLVAGSVSWLYYADRLMEFPS
                     GVLGAALGTILLPSLSKCHASNNAAEYSRLLDWGLRLTIMLTLPA"
BASE COUNT         1065 a         1734 c         1869 g         1412 t
ORIGIN      
        1 tccaccagat cgaggcggcc gatgccgtgc ttgccgccga gttcgttcag cttggccaga
       61 acctgtgccg gtgtcatctt cgtgccgttc agcgccacga tgtcgccgcg ctcgaattcg
      121 atctccagat attcggcggc atccggcgcc ttctccggcg atacgctcca gcgccacatg
      181 ctgtcttctg cttcggctgc ggggtcttcc agatggcggc cttcatagga gatgtgcagc
      241 aggttcgcgt ccatcgaata gggcgagccg ccctgcttgt gtttcatgtc gacggggatg
      301 ccatgctgct cggcataggc catcagcttt tcgcggctca aaagatccca ttcgcgccag
      361 ggggcgatga tcttgatgtt cgggttcaat gcgtaagcgc ccaactcgaa gcgaacctga
      421 tcgttgccct tgccggtcgc gccatgcgag atggcctgtg cgccggtctt ggtggcgatc
      481 tcgatcagac gcttggcgat cagcggacgc gcgatcgaag tgccgagcag gtattcaccc
      541 tcgtatacgg tattggcgcg gaacatcggg aacacgaagt cgcgcacgaa ttcttcgcgc
      601 agatcgtcga tgaaaatgct ttcaggtttg atgccgaact tcagcgcttt ctggcgcgcc
      661 ggttccagct cttcaccctg gcccaggtcg gcggtaaagg tgaccacttc gcattgatag
      721 gtgtcctgca gccatttcag gatgactgag gtgtcgaggc cgccggagta ggcgaggact
      781 actttcttga tgtcagacat aatttttaaa ccgtgttaat caatgaataa ttcaaaagaa
      841 acgataaacc ccaccatcac catcagcgaa atagcgaaaa ggaaaatgac gtcggccatg
      901 cgctcgaatt tttccgccct ttgggggtgg cgtatcgaaa agtaggacag catggcactg
      961 cccaagaaaa gcaggctatc aatagcgagc atctcattca cgtgtctgct gatgcttcgc
     1021 tgcggaatca gatgtgccag gctgataacc gtcatgcaca ccccgatcat cgtggcagac
     1081 gacggcagga tgtgatccgc caagcggctt tctccgtgct gttccgcgct gggcgtctgg
     1141 agggcggtgt cgctcatgtt gatactttgc ccagcagcag gtattccatg agagccttct
     1201 gcacatgcag tctgttttcg gcctcatccc agaccacgga ctgcgggccg tcgatgactt
     1261 ccgccgcgac ctcctcgccg cgatgggcgg gcaggcagtg catgaacagc gcatccttag
     1321 cggcgacgcg catcatgtcc gcatccacct gccagtcggc gaagtccttc atgcgctctt
     1381 cgttttcggc ttcgaagccc atgctggtcc acacatcggt ggtgaccaga tcggcgccgc
     1441 gtgccgcttc catcgggtcg gtgaattctt cgtagtgctc gttgccgtac aggcccgcgc
     1501 gctcaggctc gacctcatag ccgggcgggg tggagacatg gacgttgaag tccagtatct
     1561 cggcggcctg caaccaggta ttgcacatgt tgttggagtc gccgatccac gcgacggtct
     1621 tgccctgaat gctgccgcgc tgctcgatgt aggtgtagat gtcggcgaga atctggcagg
     1681 gatgatattc gttggtcagg ccgttgatca ccggcacgcg cgaatgcgcg gcgaagcgtt
     1741 cgatgatgga ctgttcgaag gtgcggatca tcacgatatc gctcatgcgc gagatgacct
     1801 gtccggcatc ttccaccggt tcgccgcgcc ccagttgcga gtcgcgcgtg ttcagataga
     1861 ttgccgagcc gccgagttgc tgcatgcctg cctcgaacga caggcgggtg cgggtgctgg
     1921 ctttttcgaa gatcatcacc agcgtgcgat cctgcagcgg ccagtattgc tggtaggact
     1981 tgaacctctg cttgatgacg cgggtacggt cgaacaggta ttcaagttcc tcgcggctga
     2041 gatccttgaa ttgcagaaaa tgttttacgg ctgaagaggg aatcgtaggc atgatgcact
     2101 tccaatcagg attgcaggaa ttcttttatc agtggacaca agccatctat caacaactgc
     2161 gcctcgtctt gcgacatggt cagtggcggc aatagccgga tcacgttgtc ggcggtgacg
     2221 ttgatcagca ggccacgggc cagtgccagc ttgaccagct cgccgcatgg cttgtccagt
     2281 tcgaggccga tcatcagccc ctgtccccgg atatccttga tgccatcgac gccctgcagc
     2341 gcgctggcaa agccctgacg gatgaacagg cccagcgttt cggcatgggc cagcagctgg
     2401 tcttgttcca gaatgttcag cgtggtcagt ccggccacgc aggccagcgg attgccgccg
     2461 aaggtggagc cgtggttgcc cggcttgaat gtgcccgcag ctttgcctgc ggccaggcag
     2521 gcgccgatcg gcatgcccga acccaggcct ttggccagtg tcatcacgtc gggcaggatg
     2581 ccgctgtgct ggtgggcgaa ccattttccg gtgcggccga tgccgctctg cacttcgtcc
     2641 agcatcagca gccagccgcg ttcgtcgcaa atcttgcgca actgttccag atagtggatg
     2701 tccagcgtgc ggatgccgcc ttcgccctga atcggctcga ccagtacggc gacgacatcc
     2761 ggattgtggg tggcgacctg ttcgatggcg gggatgtcgt tgtaagggac gcgcacaaag
     2821 cctttgacca gcggctcgaa accggcctgc accttgcggt tgccggtggc ggagagggtg
     2881 gccagcgtgc ggccgtggaa ggccttctcc atcacgatga tgctgggcat ctcgacgccc
     2941 ttgttgtggc cgtacatgcg cgccagtttg atggccgcct cgttggcctc gcagccggag
     3001 ttgcagaaga aggcttcctg catgccggac agggcgctca gcttgtcagc cagctgctcc
     3061 tgttcgccta cctgatagac gttggagcag tgcatcagtt tgccgatctg ctcattcagc
     3121 gcggcggtaa agcgcggatg cgcgtggccc agcgtattca cggcgatgcc ggacagtccg
     3181 tccaggtagc gacggccttc attgtcccag agccatgagc cttcgccccg cgcaaacgcg
     3241 acggggaggc gggcgtaagt gttcatcaca tgcgacatac ggaatcccta aattagaaga
     3301 cggcggacat gccgccgtcg aatcgtctgc aggttgccat tatcgtgcta aacgagtctg
     3361 caaagcaaac aagccgacat cgctgtcggc ttgtgaactg cttaccgatc cactcgccgt
     3421 taaaatccgg cagagaggct ggttaggtat tgctttagcg cgtgagcgct tatgccattg
     3481 ccttgatcgc agcagacaga cggctcttgt ggcgagctgc cttgttcttg tggatgatct
     3541 ttttgtcggc gatgctgtcc acggtgctga cagatgcctg ataaacggct tgagcagcgg
     3601 ctttgtcgcc ggcttcaacg gccttgatta ccttcttgat cgcagtgcgc aattccgaac
     3661 gcaggctgct gttgtgtgca ttctgtttta ctgcctgacg tgcacgtttg cgggcttgtg
     3721 cgctatttgc catgactact ccttgaaaat tcggtacgac gaaaggcgcg cattctagtg
     3781 gctcatccgg gcttttgcaa atctttttat cccggggcgc ttgagcgacc ctgattttac
     3841 ccctgattct gtgaatgatg gaacttctgg cgtggcgcga taaccaatgc gaccgtgcaa
     3901 ttcgatgtgc atgggcagat cgcttcgagc gctgcaacta ataacaggga aggttgaaca
     3961 tgtttcgcga acagcaagaa tgggtgatcc tcggcgtgac ggaggcggga gaggtattcg
     4021 atgtggcggg ttgggcagag cgcctgtgcg gcatgctggc caatcagggg cgggacaatc
     4081 gggtgaatta ttccgattat ctaacgcctg ctcatatcga ggggctaccc gcagtggtga
     4141 tgctgagccg tctggagcag gatgaccccg cttctttcgc tgttgtgagg cagttcgtcg
     4201 cggagaatcg gctcaagacc cgctccgggc ggagtggcag cgcgaccggc aagcatccgg
     4261 tgttgctgca ggagcgccgc tcatatatca agggctagta cggcagagcc gcgccggcag
     4321 tcgtcaatag ttcgaaatat aaggcgttgc cgttcctgcc gcttctgtgg gcgggggttg
     4381 ccagtcattc gcgggtgccg ttttttgttc cacgagcttg tttttggccg gaggagcctg
     4441 ggtgtgccgc tcctcggcgg gaggggcctg ggtcggagga tcttgcctga cctcgggggc
     4501 ggcggtctgt tcaccggctg caggttgctc gattgcggcg ggggggctcc agccgtcgtc
     4561 ggtctttgtc tcggtggttc cgtcgcgctc ttcggctttc tcgatctttt tcagttccac
     4621 gacgatggtt tgcggatagt catagcccgc gcccgatttt cgatcgacca catagccgat
     4681 cgggccgccg agcaggacat ttccccacat cccgccattg gccttggagg cgatggtttc
     4741 gctgccagag ccgacgtctt ccttgctgca ctccacggtc aggtcgtcga tgtttttgtt
     4801 gatgatgatc tggccggggg tggtgatgaa ccatctgccg acgccgttgg ccagaatgca
     4861 gcctgccgcc gtgacttcct tgtggtcctg aacggtttgc accgcgatgg tttgagtccg
     4921 ggcgccggac atggaggcgc atccggtgag ggcgctcaac atcaggatgg cgattgcgag
     4981 tcggttcatg gcagagcctt tgtgtgggat acctgtggat ttttcagtgt attggcgcga
     5041 aaagcaagct cgggaatatc tgcatggagt cgatggtatg atgcgcggcg tttataaacg
     5101 catcactctc cgcccgtatg aacctcctca aagcgcttgc tgccatcagc agtctgacac
     5161 tggtttcgcg catcctcgcc tttgcgcgcg atgtgttgat cgctcgcatc ttcggtgcgg
     5221 gaggcgcgac ggatgccttc tttgtggcgt tcaagctgcc caacctgctg cgccgcctgt
     5281 ttgccgaggg cgcgttctct caggccttcg tgccgatctt cggcgaatac aagaatcggc
     5341 gcgggcagga agagaccaag ctgctggtgg atcacgtcac caccatgctg gcgctgatcc
     5401 tgttcgtggt caccttgatc ggcatcgtcg cggcccccat tctcgtttat atcagcgcgc
     5461 cgggtttcgt taaggatgcg gctaagttcg acctcaccgt gcagctgctg cgtatcacct
     5521 cgccctatat cttcttcatc tcgctggtgg cggtggcggc gagcatcctg aatacctata
     5581 acaaattctg ggtgcccgcc ttcgcgccca ttctgctcaa cttgtgcttc atcggggggg
     5641 cactgtggct ggcaccttat tgcaacccgc ccattctggc gctggcctgg gcggtgttca
     5701 tcgcaggcat cgtgcagctg gctttccaga ttccgttcct gaagcagatc ggcatgctgc
     5761 cgacgctacg tttcaatctg aaagacgcgg gcatgtggcg ggtcatcaag ctgatggggc
     5821 ccgccgcgtt cggcgtctcc attgcgcaga tcagcctgat cgtcaacacc atcatcgctt
     5881 cgtttctggt ggcgggcagt gtgtcctggc tgtattacgc cgaccgcctg atggaattcc
     5941 cttccggcgt gctgggggca gccctgggga cgatcctgtt gccgtcgctg tccaagtgtc
     6001 acgccagcaa caatgccgcc gagtattcgc ggctgctgga ttggggcttg cgtctgacca
     6061 tcatgctgac gctgcccgcc
//
LOCUS       DASUIV010000274        15030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_538473, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000274 DASUIV010000000
VERSION     DASUIV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 15030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15030
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538473"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFW59_09875"
     CDS             complement(<1..459)
                     /locus_tag="VFW59_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohead protease"
                     /protein_id="HEX5364562.1"
                     /translation="MSAIHPSQLDQLVRHAPFDRMERTHLLWMLERMRLAYYPQGEVI
                     AAPERGVVECFFVLKQGMVQGEQNVADASEADPWLELLEGECFPLGALLAQRPVASVY
                     RAGRDTFCYELPAADFHTLLGVSAPFRDFCTRRIANLLEHSKQVIQAQYTQ"
     gene            495..950
                     /locus_tag="VFW59_09880"
     CDS             495..950
                     /locus_tag="VFW59_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5364563.1"
                     /translation="MTAIAQRQQPESTMSFSNPAPEAIRQLLHDVHSIAVLGLSPNAS
                     RPSFRVAQGLQAKGYRIIPVRPMVDTVLGEQAWPDLESLPELPDIVDVFRASEHVPAI
                     VDSCIRLGIKRLWLQDGVIDEAAALRAQAAGITVVMDRCMWRDCMQFCA"
     gene            1110..1676
                     /locus_tag="VFW59_09885"
     CDS             1110..1676
                     /locus_tag="VFW59_09885"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX5364564.1"
                     /translation="MMSRCTWPGNDALYCAYHDDEWGVPLHDDRALFEFLILEGAQAG
                     LSWITILKKRDNYRAAFDNFDATRIARFDANKIESLLQNPGIVRNRLKLAAAVVNAQH
                     FLEVQDEFGSFDAFIWRFVGGEPKQNVWRYHAEVPTRSAESDAMSKELKRRGFKFVGS
                     TICYAFMQATGMVNDHTADCFRHKELQR"
     gene            1836..2663
                     /gene="dapF"
                     /locus_tag="VFW59_09890"
     CDS             1836..2663
                     /gene="dapF"
                     /locus_tag="VFW59_09890"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5364565.1"
                     /translation="MILKFTKMHGAGNDFVVLDGVRQKVELSSEQLRFLADRHFGVGC
                     DQILLVEKPLNKDADFRYRIFNADGGEVEQCGNGARCFVRFVHDQKLTPKREIVVETH
                     SGLIRPRLEADGRVTVDMSAPVFEPARIPFQGEGAVSEPLEVAGETLAISALSMGNPH
                     AVQVVDDIEHAPVTTIGPLIEHHPRFPNRVNAGFMQVMDRHHIRLRVFERGSGETLSC
                     GTGACAAAVAGIRRGLLDSPVHVTTRGGLLTIAWNGEGTSVLMTGPAITTFEGEINL"
     gene            2673..3299
                     /locus_tag="VFW59_09895"
     CDS             2673..3299
                     /locus_tag="VFW59_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX5364566.1"
                     /translation="MRAEDVAQYLQDNPQFFEGHIDLLTQITLPHPHGGRTVSLPERQ
                     MVALREKNIGLETKLRELIEYAKENDALQHKVHDFIVALFAARDLPTLQEMIPHLLKD
                     IFAVPHAALRLWQVNPPSVEVLAFADTQTKPVCLHQAAHDTADWFGESGKQLHSFAYL
                     SLHAGNDTIGLLVLASEDKQRFYPEMGTLFLQRIAEAVSAAVHPHLNR"
     gene            3302..4234
                     /gene="xerC"
                     /locus_tag="VFW59_09900"
     CDS             3302..4234
                     /gene="xerC"
                     /locus_tag="VFW59_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX5364567.1"
                     /translation="MAVAATPAPIAAEGTNRRFLEGYLAWLRNEKRYSELTAENYARD
                     LHHLFELAADTPLASLKIHHIRRYIAQLHGKGLGGRSLARMLSAWRGFFTYLMRDHGL
                     TDNPCVGLRAPKSPKTLPQALSPDEAARLVDLPADTPEAMRDKAMFELFYSSGLRLAE
                     LVDLDLEAMRADIASGEVRVTGKGGKTRIVPLGSHAIAALQVWIGVRDQLAKTDETAL
                     FVGARGSRITPRVVQLRMKQWGIRQGITSNVHPHLLRHSFATHVLQSSGDLRAVQEML
                     GHASISTTQVYTHLDFQYLSKIYDAAHPRAKKKP"
     gene            4243..5583
                     /locus_tag="VFW59_09905"
     CDS             4243..5583
                     /locus_tag="VFW59_09905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5364568.1"
                     /translation="MTQPKSPRSPSTPHRASQGNRDFRKRTRHDAKPAAPREQVSRSI
                     VHGAADARPGIMLHAGREKSVQRRHPWIFSGAVARVDGAPNNGDTLPVRDSAGNFLAW
                     AAYSATSQITARVWSWDESEAIDAAFFRNRIGRAVAARDALKLSEHSSGLRLIHAESD
                     GLPGLIVDRYGDTLVMQIGSAGAEHWRDTIADALQALCNPVCIYERSDSDARELEGLE
                     IRNGVLRGALPERVEVVEHGLRFGVDVATGQKTGFYLDQRDNRELTGTLAKDRDVLNC
                     FCYTGGFSLYALRGGAKSVLSIDASGDALRIAEQNLTANGLDATRAEWQEADVFQALR
                     KLRDQGRSFDFIILDPPKFAPTAAFAEKAARGYKDINLLGFKLLRPGGLLATYSCSGG
                     ISEDLFQKIIAGAALDAGVDAQIMRHLHASADHPTLLSFPEGAYLKGLLLRVAG"
     gene            5753..6562
                     /locus_tag="VFW59_09910"
     CDS             5753..6562
                     /locus_tag="VFW59_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3050 domain-containing protein"
                     /protein_id="HEX5364569.1"
                     /translation="MSHRLEFSAETVSVLDEKLNNHPLYASIQTLDDLRLFMAHHVFP
                     VWDFMSLLKYLQYRIAPAAYPWKPHANSATRFFINQIVLGEESDEGLPDANGEPTYLS
                     HFELYCDAMREIGAHPSDAIHFSDVAFEQGIERALSLSTVPAAAREFVETTFGFIATG
                     KAHVVGAAFALGREHIIPEMFRSFLARMNIDEQQAPAFHYYLKRHIHLDEGFHAPLAL
                     KMMNELIDNDPEKLREAEEAAFAAVEARIKLWDGVLAAIEANRSKTSKLAA"
     gene            complement(6582..7688)
                     /locus_tag="VFW59_09915"
     CDS             complement(6582..7688)
                     /locus_tag="VFW59_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEX5364570.1"
                     /translation="MSLRLCYAATGRYLTAACVLLLCAMVNAHAAEADFLQCASKPND
                     DERLKCYDDLAWRQTNAPAAVKPEPVALPEIPSPLPAQPEVKSVDWNSRSYLTRAWNL
                     DGRAREGGAQLTPLRPYRTSYLIVRSTNSPNRQPNTPAAGHTTAVPLDLDTVESKFLI
                     SFKAEIMNYREIHWLNFRDFRLWGAYTQQSNWQAFNARSSAPFRETNYEPELIATFGT
                     ANPQGLKMVNVGFVHQSNGKQIPESRSWNRVYLQGGWEWDNNLSLLARGWWRIPETAM
                     MNDNPDLQSYLGYGDALIRWEPDTSQSVSLLLRNNLQTGQNRGFAQLDWTTPVFIGKS
                     AKLHAQVSNGYGESLIDYNHPQTTFGLGISFREW"
     gene            complement(7711..8643)
                     /locus_tag="VFW59_09920"
     CDS             complement(7711..8643)
                     /locus_tag="VFW59_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX5364571.1"
                     /translation="MVKQITILTLATLVLAACGSTPQRNAETRNTPAPIDSRPVGPVG
                     GGSGAGGYLAGDGPGANAPANLDAIPDAVPKSEPLHRYANRPYVALGKTYTPQTIAGN
                     YKDRGIASWYGKKFHGQRTSSGETYDMYGMTAAHPTLPIPSYARVTNVTSGKSVVVRV
                     NDRGPFLHDRVMDLSYSAAYKLGIIGNGSAEVEVESLAPNAIVSNVARAEPVQTRPLE
                     KSKPAKAPTLAAASIGGNVYLQLGAFKSREAAESFMAKMRAELGDVGKQLSVYMQGGL
                     TRVHVGPYANQNEARSRAEGFQDRLGFKPMVNLH"
     gene            complement(8766..9863)
                     /gene="rodA"
                     /locus_tag="VFW59_09925"
     CDS             complement(8766..9863)
                     /gene="rodA"
                     /locus_tag="VFW59_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX5364572.1"
                     /translation="MTKRQWWQRFIAHIDPPLMLGLGLLMLTSLVVLYSADDGHWGQP
                     LKQLLNIMVGLFCMWVVANLPLHYLMRTAVPVYVLGMVLLIGVALFGEISHGARRWLN
                     LGIITIQPSELMKIGVPLMMAWYFEKNEATLTGKNFFIAALLLLVPVGLIARQPDLGT
                     AILISASGFYVLFLAGLSWRVMGGMAVAVLASTPFMWAMLHDYQRHRIEMLLDPSKDA
                     LGKGYHTIQGMIAVGSGGVFGKGYLEGTQTHLDFLPERTTDFIFAVFSEEFGLIGNLM
                     LIGMFLLVIGRGFIITANASTYFTRLMAGSITLTFATYAFVNMGMVSGILPVVGVPLP
                     MVSYGGTSMLTLLLGFGMLMSIETHKKLNKT"
     gene            complement(9856..11754)
                     /gene="mrdA"
                     /locus_tag="VFW59_09930"
     CDS             complement(9856..11754)
                     /gene="mrdA"
                     /locus_tag="VFW59_09930"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5364573.1"
                     /translation="MSKSVELKNHQREIFYFRLRLAIGIGFVLMLMAILLARFIYLQV
                     VRHDYYQTLAESNRISIVPIVPNRGLILDRNGEVLAHNYSGYTLEITPTKTKGLEATI
                     NELATLVDITPRDRKRFKKLLAERRNFESLMIRNRLSDEEVARFAAQQYRFPGVEIKA
                     RLFRDYPYGEGTAHLIGYIGRINQTDVDQLEENDLAANYRGSDYIGKTGLEQSYESEL
                     HGKTGVEQVEVDSGGRGVRILSRSPPSSGNTLVLSIDAKLQDIAVKAFGDYRGALVAI
                     DPNNGEVLAFVSKPGYDPSLFVDGIDPQSWDELNNSPDTPLNNRALRGQYPPGSTIKP
                     FMALAGLHYGRSPSYTIGDPGYYTLQGSGHQYRDWKKGGHGTVDMFKSIVVSCDTYYY
                     GLATELGIDNIFNYLSGFGFGKKTGIDLEGETSGLLPSQEWKMRRYQQKWYAGDTVSV
                     GIGQGYNLVTPMQLAFATATLANNGVAYRPHLVREVRSSREAENRVIASKPEFDLKLD
                     PAHYDLVRRAMIAVTQPGGTAVAASAGASYQIAGKTGTAQVVGMKQGEKYDASKLDER
                     HRDHAWFIAYAPAEQPKIALVVLAENGGHGGGTAAPIARKVLDYYLLGKVPQPLPDAA
                     DKEVVEHD"
     gene            complement(11751..12263)
                     /gene="mreD"
                     /locus_tag="VFW59_09935"
     CDS             complement(11751..12263)
                     /gene="mreD"
                     /locus_tag="VFW59_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5364574.1"
                     /translation="MPKLSSGAPEIRRPVSNVFIVLTILTALFLNALPWEGVWLLLRP
                     DFVAVVLLYWCTHNPLRIGVGLSWLVGMLADVADSSLFGQHALAYTLLAFGGVVLHRR
                     LQMFDVRQQTVQVLGIFALTYGVYALVHWQVNGYVMWAYFIGCLTSTLLWAPLSILFR
                     AVQQARVDRE"
     gene            complement(12263..13174)
                     /gene="mreC"
                     /locus_tag="VFW59_09940"
     CDS             complement(12263..13174)
                     /gene="mreC"
                     /locus_tag="VFW59_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5364575.1"
                     /translation="MDSTQSFQIFNRGPSPAARLVFFAVLSLLLMFVDARYSYLGAAR
                     GALSVLVAPIQQLATMPSKLWHQAGDFFVTQSALVEENGNLRQRHSYDAAQLSQLQSL
                     QQENQQLRNLLVLPLRASLSAQAAEIVYAERDVFKRKLLINKGMNSRIKVGQVVVDDM
                     GIVGQITRAYPWLAEVTLITEKDHAVPVQLVRNGLRSIVFGAGDTSQLSLRYMPISSD
                     IQNGDVLVTSGIDGVYPPGVPVAKVVRIERDAAYPFARITCLPMAGVDRNRHLLVLSD
                     LPKLPEPPAEEAPPALTDKAARTGGPH"
     gene            complement(13262..14305)
                     /locus_tag="VFW59_09945"
     CDS             complement(13262..14305)
                     /locus_tag="VFW59_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5364576.1"
                     /translation="MFGFLNGYFSNDLAIDLGTANTLIWVRGQGIVLDEPSVVAIRQE
                     GGPNGKKVIQQVGLAAKQMLGRTPGNITAIRPMKDGVIADFTVTEQMLKQFIRKVYKA
                     GIFSPSPRIVICVPCGSTQVERRAIRESAVGAGARRVELIEEPMAAAIGAGLPVEEAT
                     GSMVVDIGGGTTEVGVISLGGAVYSGSVRVGGDKFDEAIINYIRRNYGMLIGETTAED
                     IKKTIGSAFPGSEVREMEVKGRNLAEGVPRSFTISSNEILEALTDPLNSIVSAVKTAL
                     EQTPPELGADIANKGMVLTGGGALLRDLDRLLMEETGLPVLIAEDPLTCVVRGSGMAL
                     ESMDKSTSIFTTE"
     gene            14387..14674
                     /gene="gatC"
                     /locus_tag="VFW59_09950"
     CDS             14387..14674
                     /gene="gatC"
                     /locus_tag="VFW59_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5364577.1"
                     /translation="MSLTTQDVQKVARLARLAMNEAEIEAARAQLSGIFTLIAEMQAV
                     NTTGITPMSHAQDVAQRLRADEVTATNQREAFQAIAPQVEGGLYLVPKVIE"
     gene            14678..>15030
                     /locus_tag="VFW59_09955"
     CDS             14678..>15030
                     /locus_tag="VFW59_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5364578.1"
                     /translation="MLESSLQQQGAALRAKKISSVELTQLYLDRIAALNPQLNAYITL
                     NPEMTLAQAREADSRLAGGSTDALTGIPIAQKDIFCAKGWLTTCGSKMLHNFVSPYDA
                     HVIEQFNRAGAVNLG"
BASE COUNT         3016 a         4843 c         4367 g         2804 t
ORIGIN      
        1 ctgggtgtac tgcgcctgaa tgacctgctt ggagtgttcg agcagattgg cgatacggcg
       61 cgtgcagaaa tcgcggaacg gcgcgctgac cccgagcagc gtatgaaaat ccgccgccgg
      121 caattcgtag cagaacgtat cacggcccgc gcggtagaca ctggcgaccg ggcgctgtgc
      181 cagcagcgcg ccgagcggaa agcactcgcc ctccagcagc tccagccagg gatcggcttc
      241 ggaggcgtcc gcgacattct gctcgccctg caccatgccc tgcttgagta cgaaaaaaca
      301 ctccaccact ccgcgctccg gcgcggcgat cacctcgccc tgcggataat aggcaaggcg
      361 catgcgctcc agcatccaca gcagatgggt gcgctccata cggtcgaacg gggcgtgcct
      421 gacaagctgg tccagctggg acggatgtat cgcgctcacg ggtgcggcct cttcgtatca
      481 tgcaatggtt tattatgact gccatcgctc aacgccaaca acccgaatcg accatgagtt
      541 tcagcaatcc cgctcccgaa gctatccgcc aactgctgca cgacgtgcac agcatcgccg
      601 tgctgggcct gtcgccgaac gcctcgcgcc ccagcttccg tgtcgcccaa ggcttgcagg
      661 ccaagggtta ccgcatcatt ccggtgcgcc cgatggtcga taccgtgctg ggcgaacagg
      721 cctggcccga tctggaaagc ctgccggagt tgccggacat cgtcgacgtg ttccgcgcgt
      781 ccgaacatgt gcccgccatc gtggacagct gcatcaggct cggcatcaag cgcctgtggc
      841 tgcaggatgg cgtgatcgac gaagcggcgg cattgcgcgc gcaggccgcc ggcatcaccg
      901 tggtgatgga tcgctgcatg tggcgcgact gcatgcagtt ctgcgcctga tcccagtaaa
      961 acagttgtcc acgaaaatca cgaaatcaca cgaaccaaga tcaatagact tatgttaaga
     1021 aaaattccaa cgggcaagtc tcaaagcgat ggaaccagct ggttttattt cgtgccgttc
     1081 gtgcatttcg tggatgattt actattttta tgatgagtcg ctgcacctgg cccggcaatg
     1141 atgcgctgta ttgcgcctat cacgacgacg aatggggcgt gccgctgcac gacgaccgcg
     1201 cgctgttcga gtttctgatt ctggagggcg cgcaggctgg cctgagctgg atcaccatcc
     1261 tgaagaagcg cgacaactac cgcgccgcct tcgacaattt cgatgcgacg cgcattgccc
     1321 gctttgacgc gaataaaata gaatcgctgc tgcagaatcc cggtatcgtg cgcaaccggc
     1381 tgaaattggc tgcggcggtg gtgaatgcgc agcattttct cgaggtgcag gatgagttcg
     1441 gcagcttcga cgccttcatc tggcgcttcg tcggcggcga accgaaacag aatgtctggc
     1501 gctatcatgc cgaggttccg acccgcagcg cggaatcgga tgcgatgagc aaggagctga
     1561 agcggcgcgg tttcaagttc gtcggctcga cgatctgtta tgccttcatg caggcgaccg
     1621 gcatggtgaa cgaccacacg gcggattgct tcagacataa agaattgcag agataaacca
     1681 aaaatccatc cgcagattgc acagattgac gcacattaga cccagcctat ccatcgggcc
     1741 aatctctccg ctggagagac tggccgccat gatttattga atctgtttga taatccatct
     1801 aatctgcgca atctgcggac aaccaaggtt ttgagatgat tttaaaattt accaagatgc
     1861 acggtgcggg caacgacttc gtcgtactgg acggcgtgcg ccagaaggtc gaactctcgt
     1921 ccgaacaatt gcgttttctc gccgaccgcc acttcggcgt gggctgcgac cagattctgc
     1981 tggtcgagaa accgctgaac aaggatgccg atttccgcta ccgcatcttc aatgccgacg
     2041 gcggcgaggt cgaacagtgc ggcaacggcg cgcgctgctt cgtgcgcttc gtgcacgacc
     2101 agaaactcac gcccaaacgc gaaatcgtgg tcgagacgca cagcggcctg atccgcccgc
     2161 gcctggaggc ggacgggcgc gtcacggtgg acatgagcgc gccggtgttc gaacccgccc
     2221 gcattccgtt ccagggcgaa ggtgcagtca gcgaaccgct ggaagtggcc ggtgagacac
     2281 tggctatcag cgccctgtcg atgggcaacc cgcatgcagt tcaagtcgta gacgacatcg
     2341 aacatgcgcc ggtgacgacg atcgggccgc tgatcgagca tcacccgcgc ttccccaatc
     2401 gcgtcaacgc cggtttcatg caggtgatgg accgccatca catccgcctg cgcgtgttcg
     2461 agcgcggctc gggcgagacc ctgtcctgcg gcaccggcgc ctgcgccgct gccgtcgcag
     2521 gcatccggcg cggcctgctg gacagcccgg tgcatgtcac cacccgtggc ggcctgctga
     2581 ccatcgcctg gaacggcgaa ggcacatcgg tactgatgac cggcccggcg atcaccacat
     2641 tcgaaggcga aatcaattta tagggagtca ccatgagagc agaagacgtc gcacaatatt
     2701 tgcaggacaa cccgcagttc ttcgagggcc atatcgatct gctcacccag atcacgctgc
     2761 cccatccgca tggcggccgc accgtctcgc tgcccgaacg gcagatggtg gcgttgcgcg
     2821 aaaagaatat cgggctggaa accaagctgc gcgagctgat cgaatacgcc aaggaaaacg
     2881 atgcgctgca gcacaaggtg catgacttca tcgtcgccct gttcgccgcg cgcgacctgc
     2941 ccaccctgca ggaaatgatc ccgcacctgc tgaaagacat cttcgccgtg ccgcatgccg
     3001 cgctgcgcct gtggcaggtc aacccgccca gcgtggaagt gctggccttc gccgatacac
     3061 agaccaagcc ggtctgcctg catcaggcgg cacacgacac cgccgactgg ttcggcgaga
     3121 gcggcaagca gctgcattcc ttcgcctacc tgtcgctgca tgcgggcaac gacaccatcg
     3181 gcctgctggt gctggccagc gaagacaagc aacgcttcta ccccgagatg ggcacgctgt
     3241 tcctgcaacg catcgccgag gcggtgagcg ccgcagtaca ccctcatctc aatcgctaac
     3301 catggccgtt gccgccacgc ccgcccccat cgctgccgaa ggtaccaacc ggcgattcct
     3361 tgagggttat ctggcgtggc tgcgcaatga gaaacgctat tccgagctga cggccgaaaa
     3421 ctacgcgcgc gatctgcatc atctgttcga actcgccgcc gacaccccgc tcgccagcct
     3481 gaagattcac cacatccgcc gctacatcgc gcagctgcac ggcaaggggc tgggcggacg
     3541 ctcgctggcg cgcatgctgt ccgcctggcg cggatttttc acctatctga tgcgcgatca
     3601 cggcctgact gacaatccct gcgtcggcct gcgcgcgccg aagtcgccca agaccctgcc
     3661 tcaggcgctg tcgccggacg aggcggcgcg gctggtcgac ctgcccgccg acacgcccga
     3721 ggcgatgcgc gacaaggcga tgttcgaact gttctattcg tccggcctgc gtctggccga
     3781 actggtcgat ctcgaccttg aagcgatgcg cgccgacatc gcgtcgggcg aggtgcgcgt
     3841 caccggcaag ggcggcaaga cccgcatcgt gccgctgggc agccacgcca tcgccgcgct
     3901 gcaagtctgg atcggcgtgc gcgaccagct cgccaaaacg gacgagaccg cgctgttcgt
     3961 cggcgcgcgc ggcagccgca tcacgccgcg cgtggtgcaa ctgcgcatga agcaatgggg
     4021 catcagacag ggcatcacca gcaacgtgca tccgcacctg ctgcgccact cattcgccac
     4081 ccatgtgctg caatcttccg gcgacctgcg cgcggtgcag gagatgctcg gccacgccag
     4141 catctccacc acgcaggtct acacccatct cgacttccag tatctgagca aaatctacga
     4201 cgcggcccat ccgcgcgcca agaaaaaacc ttaaggacaa acatgaccca gccaaaatcg
     4261 ccgcgctccc cctccacccc gcaccgcgcc tcgcagggga accgggattt ccgcaagcgc
     4321 acccggcacg atgccaagcc ggccgcaccc cgcgaacaag tttcgcgcag catcgtccac
     4381 ggagcggcgg acgcgcgacc cggcatcatg ctccacgccg gtcgcgagaa gtctgtgcaa
     4441 cgccgccacc cctggatatt ctccggcgcg gtcgcccgcg tggacggcgc gccgaacaac
     4501 ggcgacaccc tgccggtgcg cgatagcgcc ggaaacttcc tcgcctgggc ggcctacagc
     4561 gcgacctcgc aaatcaccgc gcgcgtctgg tcatgggacg aatcggaggc catcgacgcc
     4621 gcgttcttcc gcaaccgcat cggccgcgcg gtcgcggccc gcgacgcgct caaactgagc
     4681 gagcacagca gcggcctgcg cctgatccac gccgaatccg acggtctgcc gggcctgatc
     4741 gtggatcgct acggcgacac gctggtgatg cagatcggca gcgcgggggc ggaacactgg
     4801 cgcgacacca tcgccgacgc actgcaggcg ctatgcaatc ccgtttgtat ctacgagcgt
     4861 tcggattccg atgcccgcga actggaaggg ctggagattc gcaacggcgt gctgcgcggc
     4921 gccttgcccg aacgcgtgga agtcgtcgaa cacggccttc gcttcggcgt ggatgtggcg
     4981 acaggccaga agaccggctt ctacctcgac cagcgcgaca accgcgaact gaccggcacg
     5041 ctggcgaagg accgcgacgt gctgaactgc ttctgctaca ccggcggctt ctcgctgtac
     5101 gcgttgcgcg gcggcgcaaa atcggtgctg tccatcgacg cctccggcga cgcgctgcgc
     5161 atcgccgaac agaatctgac cgccaacgga ctggatgcca cacgcgccga atggcaggag
     5221 gccgacgtgt tccaggcgct gcgcaaattg cgcgatcagg gccgtagctt cgacttcatc
     5281 atcctcgatc cgccgaagtt cgcccccacc gccgccttcg ccgaaaaagc cgcacgcggc
     5341 tacaaggaca tcaacctgct cggcttcaaa ctgctcagac cgggcggcct gctcgccacc
     5401 tactcatgct ccggcggcat ctctgaggac ctgttccaga aaatcatcgc cggagccgca
     5461 ctggatgcgg gcgtggacgc ccagatcatg cgccacctgc acgccagcgc cgatcacccg
     5521 acgctgctga gttttcccga gggggcctat ttgaaggggt tgttgctgcg ggtagcaggg
     5581 tagcgagttg ggttagcagg taggatgggt tgagcatagc gatacccatc aagtcaaatc
     5641 aaaacccccg gcggttttga tgttgggtta cgcgtaaccc gactgccctg ccccaatcgc
     5701 ctgtccgagt tgtgatatat tagaaaattc taatttcata taggggccga cgatgtcgca
     5761 tcgccttgag ttcagcgcag agaccgtttc cgtcctcgac gagaaactca acaatcaccc
     5821 gctatatgcc tcgatacaga cgttggatga tctgaggcta ttcatggctc atcacgtctt
     5881 cccggtatgg gatttcatgt cgctgctgaa atatctgcaa taccggattg cacccgccgc
     5941 atacccgtgg aaacctcatg caaattcggc cacccgcttc ttcatcaatc agatcgtgct
     6001 tggcgaggaa tccgacgaag gtctgcccga tgccaacggc gagccaacct atctgagcca
     6061 cttcgagctt tactgcgacg cgatgcgtga aatcggagcg catccttccg acgcaataca
     6121 tttttccgat gtcgcgttcg agcagggcat cgaacgcgcg ctgtcgctca gcaccgttcc
     6181 cgctgcggca cgcgaattcg tggagacaac cttcggcttt atcgccaccg gcaaagccca
     6241 tgtcgttggc gccgcatttg cactggggcg ggagcacatc atcccggaga tgttccggtc
     6301 gttccttgca cggatgaaca tcgacgagca gcaagcccct gcattccatt actatctgaa
     6361 gcgccacatt catctggatg aaggcttcca tgcgccactg gcattgaaga tgatgaacga
     6421 gctgatcgac aacgatccgg agaagctgcg cgaagcggaa gaggcggcat tcgccgcagt
     6481 agaggccagg atcaagctat gggacggcgt tctcgccgcg attgaagcga accgcagcaa
     6541 gacatcgaag ctggcggctt agcgcatctg aaacagcgga actaccactc cctgaacgaa
     6601 atccccagcc cgaacgtggt ctggggatgg ttgtagtcga tcaggctttc gccgtagccg
     6661 ttgctgacct gtgcgtgcag cttggccgac ttgccgatga ataccggcgt cgtccagtcg
     6721 agctgggcga atccgcggtt ctgcccggtt tgcagattat tgcgcagcag cagcgagacc
     6781 gactgactcg tatccggctc ccaacgtatc aaggcatcgc cgtatcccag ataactttgc
     6841 agatccggat tatcgttcat catcgctgtt tccggaatgc gccaccagcc gcgcgccaac
     6901 agcgacaggt tgttgtccca ttcccagcca ccctgcagat acacgcggtt ccagcttctg
     6961 gattccggta tctgtttgcc gttcgattgg tgcacaaagc ccacgttgac catcttcagg
     7021 ccttgcggat tggccgtacc gaaagtggcg atcagttccg gctcgtaatt ggtctcccgg
     7081 aacggagcag aactgcgcgc attgaacgcc tgccaattgg actgctgggt gtaagcgccc
     7141 cacagccgga agtccctgaa attcagccaa tggatttccc ggtaattcat gatttcagcc
     7201 ttgaagctga tcaggaattt cgactcgacg gtatcgagat ccagcggcac cgctgtcgtg
     7261 tggccggccg caggtgtatt gggctggcga ttcggactgt tcgtgctgcg cacgatgaga
     7321 tagctggttc gatagggcct gagcggcgtc agttgcgcgc ctccctctct ggcccggcca
     7381 tccagattcc acgcacgagt aagataactg cggctattcc aatcgacgct tttgacttca
     7441 ggctgtgcgg ggaggggcga cggaatctcg ggcagcgcaa ctggctcggg cttgaccgcc
     7501 gccggtgcat tagtctgccg ccaggcgaga tcgtcatagc acttcagccg ctcatcgtca
     7561 ttgggtttgc tcgcgcattg caggaaatcc gcctctgccg catgggcgtt aaccatcgca
     7621 cacagcagca acacgcaggc tgccgtcagg taacgacccg tcgctgcata gcagagcctg
     7681 agggacatgt ctttttccag aacggcgaaa ttaatgcagg ttcaccatcg gtttgaagcc
     7741 cagcctgtcc tgaaagccct cggccctgct gcgcgcctca ttctgattgg cataggggcc
     7801 gacatggaca cgggtcaacc cgccctgcat gtacacgctg agctgtttgc cgacatcgcc
     7861 cagttccgcg cgcatcttcg ccatgaaact ctccgccgct tcgcgcgact tgaaggcgcc
     7921 cagttgcagg tagacattgc cgccgatgga cgccgctgcc agcgtcggtg ccttggccgg
     7981 cttggatttc tccagcggtc tggtctgcac cggctcggct ctggcgacat tgctgactat
     8041 cgcgttcggc gccaggcttt cgacttccac ctcggcactg ccgttaccga tgatgcccag
     8101 cttgtaggct gcgctgtagg aaagatccat cacgcggtca tgcaggaagg ggccgcgatc
     8161 gttgacgcgc accaccacgg acttgccgct cgtcacgttg gtgacacgcg cataactcgg
     8221 aatcggcaac gtggggtgag ccgcagtcat gccgtacatg tcgtaggtct cgccgctgga
     8281 ggtgcgctga ccgtggaatt tcttgccgta ccaggaggcg atgccgcgat ccttatagtt
     8341 gccggcgatg gtctgcgggg tgtaggtctt gccgagcgcg acatagggac ggttggcata
     8401 gcgatgcagg ggttcactct tcggcacggc atccggaatc gcgtccagat tggccggtgc
     8461 attcgccccg gggccgtcgc ccgcgagata gccacccgca ccagagccgc cgccgaccgg
     8521 gccgaccggg cggctgtcaa ttggcgcggg cgtgttgcgc gtttcggcat tgcgctgcgg
     8581 cgtactgccg cacgcggcca gcaccagcgt tgccaaagtc aaaatcgtga tctgcttaac
     8641 catactcgct ccttttaatc tgccacccgg cttccgttaa cctgcggctt atatcgtcgt
     8701 gttgagaccg gcccggccaa tgcccctgca aggtcgttgc gagcccgatg gatggacggt
     8761 ttttatcaag ttttgttcaa tttcttgtgc gtctcgatgc tcatgagcat accaaaaccc
     8821 agcagcaggg tgagcatcga tgtcccgccg tagctgacca taggcaaggg cacgcccacc
     8881 acgggcagga taccactgac catccccatg ttgacgaagg cataggtggc aaaggtcaac
     8941 gtgatggaac cggccatcag gcgcgtaaaa taggtggagg cattggcggt gatgatgaag
     9001 ccgcgtccga tcaccagcag gaacatgccg atcagcatca agttgccgat caggccgaac
     9061 tcctcggaaa acaccgcgaa gataaagtcg gtggtgcgtt ccggcagaaa atcgagatgc
     9121 gtctgcgtac cctccagata acccttgccg aagacaccgc cggaacccac cgcgatcatg
     9181 ccctgtatgg tgtggtaacc cttgcccagc gcatccttcg aagggtcgag cagcatctcg
     9241 atgcgatggc gctggtagtc gtgcagcatc gcccacatga atggcgtgct ggcgagaacg
     9301 gcaacggcca tgccgcccat cacccgccag gacaagcccg ccagaaacaa cacatagaaa
     9361 ccgctggcgg aaatgagaat ggccgtgccc agatcgggct ggcgcgcgat caggccgacg
     9421 ggaaccagca gcaacaaggc ggcgataaaa aaattcttcc cggtcagcgt ggcctcgttc
     9481 ttctcgaagt accaggccat catcagcggc acgccgatct tcatcagttc ggacggctgg
     9541 atggtaatga tgccgagatt cagccagcgc cgtgcgccgt gactgatctc gccgaatagc
     9601 gcgacgccga tcagcaacac catccccagc acgtagaccg gcacggcggt gcgcatcagg
     9661 taatgcaatg gcaggttcgc caccacccac atgcagaaca gccccaccat gatgttgagc
     9721 aactgtttca gcggctgccc ccaatgcccg tcgtccgcgc tatacaacac caccagactg
     9781 gtgagcatca acagcccgag ccccagcatc aacggcggat cgatgtgcgc gatgaaacgc
     9841 tgccaccact gacgtttagt catgttccac gacctccttg tcggccgcat ccggcaacgg
     9901 ttgcggcacc ttgccgagca gatagtaatc cagcaccttg cgtgcgatcg gcgccgccgt
     9961 gccgccgcca tgcccgccat tctcggccag cacgaccagt gcgattttgg gctgctcggc
    10021 tggcgcgtag gcgatgaacc atgcgtggtc gcgatggcgt tcgtccagct tgctggcatc
    10081 gtatttctcg ccctgcttca tcccgaccac ctgcgcggta cccgtcttgc ccgcgatctg
    10141 ataggacgcg cccgccgagg ccgccacagc cgtgccgccc ggctgggtga ccgcgatcat
    10201 cgcgcgccgc accagatcgt aatgagccgg atcgagcttg agatcgaact cgggcttgct
    10261 cgcgatcacg cggttctcgg cctcgcgcga actgcgcacc tcgcgcacca gatgcgggcg
    10321 gtaggccacg ccgttatttg ccagcgtcgc ggtcgcgaag gccagctgca tcggcgtcac
    10381 cagattgtag ccctgcccga taccaaccga caccgtgtct cccgcatacc atttctgctg
    10441 gtagcgcctc atcttccatt cctgcgaagg cagcaggccg gaggtctcgc cttccagatc
    10501 gatgccggtc ttcttgccga aaccgaaccc cgacagatag ttgaatatgt tgtcgatgcc
    10561 caactcggta gccaacccat aatagtaggt gtcgcaggac accacgatgg acttgaacat
    10621 atccaccgtg ccgtgccccc cttttttcca gtcgcggtac tgatgaccgc taccctgcag
    10681 ggtgtaatag cccgggtcgc cgatggtata actcggcgag cggccgtaat gcaatccggc
    10741 caaggccatg aacggcttga tggtggaacc cggcggatac tgcccgcgca gcgcccggtt
    10801 gttcaacggg gtatccggtg aattgttcag ctcgtcccaa ctctggggat cgataccgtc
    10861 gacgaacagg ctggggtcat agcccggctt gctgacgaag gccagcacct cgccgttgtt
    10921 cggatcgatc gccaccaagg cgccgcgata atcgccgaac gccttgaccg cgatatcctg
    10981 caacttcgca tcgatactga gcaccagggt attgccggag gacggcgggc tgcgcgacag
    11041 gatgcgcacg ccgcgcccgc ccgaatccac ctcgacctgc tccaccccgg tcttgccgtg
    11101 cagctcgctt tcgtagctct gctccagacc ggtcttgccg atgtaatcgg agccccggta
    11161 attggcggcc agatcgttct cttccagctg atcgacatcg gtctggttga tgcggccgat
    11221 atagccgatc agatgtgccg tgccctcgcc gtagggatag tcgcggaaca gccgggcctt
    11281 gatctcgacc ccggggaaac ggtattgctg ggcggcgaaa cgcgccactt cctcgtcgct
    11341 gaggcgattg cgtatcatca ggctttcgaa gttgcgccgt tccgccagca gcttcttgaa
    11401 acgcttgcga tcgcgcggcg tgatgtccac cagcgtggcc agctcgttta tcgtggcttc
    11461 cagccccttg gtcttggtcg gcgtgatttc cagcgtatag ccggaatagt tatgcgccag
    11521 cacctcgccg ttgcggtcca gaatcaggcc acggttcggc acgatgggga cgatggaaat
    11581 gcgattgctc tccgccagcg tctggtaata atcgtggcgc accacttgca gatagatgaa
    11641 acgcgccagc agaatcgcca tcagcatcag cacgaagccg ataccgatcg ccaatcgcaa
    11701 ccggaaatag aatatctcgc gctggtgatt ctttaattcg acgctcttgc tcattcacga
    11761 tccacgcgcg cctgttgcac cgcccggaac aggatgctga gcggcgccca cagcaaggtc
    11821 gatgtcaggc aaccgatgaa atacgcccac atcacatagc cgttcacctg ccagtgcacc
    11881 agcgcgtaca ccccataggt caatgcaaag attcccagca cctgcacggt ctgctggcgc
    11941 acgtcgaaca tctgcaaacg acggtgcaac acgacgccgc cgaaagccag cagcgtgtag
    12001 gccagcgcat gctgaccgaa caggctggag tccgccacat cggccagcat gcccaccagc
    12061 caggacaaac cgacacctat cctcagggga ttatgcgtgc accagtacag cagcaccacc
    12121 gcgacgaagt ccgggcgcaa tagcagccag acaccttccc agggcagcgc attcaggaac
    12181 agcgccgtca gtatcgtcag cacgatgaag acattgctga ccggacgccg gatctccggc
    12241 gcgcccgatg agagcttcgg catcagtgtg gccctccggt tctggcagcc ttgtccgtga
    12301 gcgctggcgg cgcctcctct gcgggaggct cgggcagttt tggcagatca gacaagacca
    12361 gcagatgtcg attgcgatcc actccggcca ttggcaaaca ggtgatgcgt gcaaacgggt
    12421 aggcggcgtc ccgctctatc ctgacgacct tggcaaccgg cacgcccggc ggatagacgc
    12481 catcgatgcc cgaggtcacc agcacatcgc cattctgaat gtcggaactg atcggcatgt
    12541 agcgcaacga caactggctg gtgtcgcccg caccgaacac gatggaacgc aagccattgc
    12601 gcaccagctg caccggcacc gcgtgatcct tctcggtgat cagtgtcacc tcggccagcc
    12661 agggataagc gcgggtgatc tgcccgacga tgcccatatc gtcgaccacc acctgaccga
    12721 ccttgatccg ggaattcatc cccttgttga tcagcagctt gcgtttgaat acgtcccgct
    12781 cggcatacac gatctcggcc gcctgcgcag aaagactggc gcgcagaggc aacaccagca
    12841 ggttgcgcaa ttgctggttc tcttgctgca aggattgcaa ctgcgacagc tgggcggcgt
    12901 cgtagctgtg tcgttgccgc aaattgccgt tttcctcgac cagggcactc tgggtgacga
    12961 agaaatcacc cgcctgatgc cataacttgc ttggcatggt tgccagctgc tggataggcg
    13021 cgaccagcac cgacaaggcc ccccgcgccg cgcccagata gctgtagcgc gcgtcgacga
    13081 acatcagcaa caaggaaagc accgcgaaga ataccagccg cgccgcaggg gacgggccgc
    13141 gattgaagat ctgaaaggac tgcgtgctat ccatcggcta gggcgggtgg tgagtagcaa
    13201 gtagagagcg gtaagtaaaa agctggtttc cctgccactc cccacccacc acttaccaga
    13261 tttattcggt ggtaaagatg ctggtggact tgtccatgct ctccagcgcc atgccggaac
    13321 cgcgcaccac gcaggtcaaa ggatcctcgg cgatcagcac cggcagcccg gtctcttcca
    13381 tcaacaggcg gtccagatcg cgcagcagcg cgccgccgcc agtcagcacc atacccttgt
    13441 tggcgatgtc cgcgccgagt tcgggcggag tctgttccag cgccgtcttc accgcgctga
    13501 cgatgctgtt gagcgggtcg gtcagcgcct cgagaatttc gttgctggaa atggtgaagc
    13561 tgcgcggcac gccttcggcc aggttgcgac ccttcacttc catctcgcgc acctcggaac
    13621 ccgggaacgc ggaaccgatg gtcttcttga tatcttcggc agtcgtctcg ccgatcagca
    13681 tgccgtagtt gcggcggatg taattgatga tcgcctcgtc gaacttgtcg ccgccgacgc
    13741 gcaccgaacc ggaataaacc gcaccgccca gcgagatcac gccgacttct gtggtgccgc
    13801 cgccgatgtc caccaccatc gagccagtcg cttcctcgac cggcaggcct gcgccgattg
    13861 cggcagccat cggctcctcg atcaactcca cgcgacgcgc gccggcgccg actgcggatt
    13921 cgcggatcgc gcggcgctcg acctgggtcg aaccgcaggg cacgcagatc acgatgcgcg
    13981 gactgggact gaagatgccc gccttgtaga ccttgcggat gaactgcttg agcatctgct
    14041 cggtcacggt gaagtcggcg atcacgccat ccttcatcgg acggatcgcg gtgatgttgc
    14101 ccggcgtacg gcccagcatc tgcttggcgg ccagaccgac ctgctggatc accttcttgc
    14161 cgttcggccc gccctcctga cggatcgcca ccaccgaagg ctcatccagc acgatgccct
    14221 gcccgcgtac ccagatcaga gtattcgcgg tgccgaggtc aatcgcaagg tcattggaaa
    14281 agtagccgtt caaaaatcca aacatgatgt atcccgtagt caaagaggtg cgctgataaa
    14341 cccggttatg ataacctatc tatcttgttc caataaagct tttgccatgt ctttaactac
    14401 tcaggatgtt caaaaagtcg cccgtctcgc ccgtttggcg atgaacgagg ccgaaatcga
    14461 ggctgcccgc gcccagttgt cgggtatttt taccctgatc gccgagatgc aagccgttaa
    14521 caccacgggc atcaccccga tgtctcacgc ccaggatgtc gcccagcggc tgcgcgcgga
    14581 cgaagtcacc gcaaccaatc agcgcgaggc ttttcaggcc atcgccccgc aggtcgaagg
    14641 cggcctgtac ctcgttccga aagtcatcga ataaaccatg cttgaatcca gtttgcagca
    14701 acagggcgcc gcactgcgcg ccaagaagat ctccagcgtc gagctgacac agctctacct
    14761 cgaccgtatc gccgcgctca atccgcagct caatgcctat atcacgctga acccggagat
    14821 gacactggca caggcacgag aggccgactc ccgtctggcg ggcggatcga ccgacgcgct
    14881 gaccggcatc cccatcgccc agaaggatat cttctgcgcc aagggctggc tcaccacctg
    14941 cggctcgaag atgctgcaca acttcgtttc gccttacgac gcccatgtca tcgagcaatt
    15001 caaccgcgcg ggcgcggtga atctcggcaa
//
LOCUS       DASUIV010000275         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_352393, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000275 DASUIV010000000
VERSION     DASUIV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352393"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(538..1602)
                     /locus_tag="VFW59_09960"
     CDS             complement(538..1602)
                     /locus_tag="VFW59_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX5364579.1"
                     /translation="MKHEVNKGRGATLRIEGRFESVARERVDDGWTVDDELPPLKTTV
                     TEERAKSVIQQQNSPDLPFALSINAYRGCEHGCIYCYARPSHGYLNLSPGLDFETRLF
                     AKPEAARLLRAELAKPSYRCSPIALGVNTDAYQPIERDWKITRQILEVLAECRHPLTI
                     VTKSALIERDLDLLTQLAQDNLVQVFISVTTLDAELARRLEPRAASPRRRLQTMQRLN
                     EAGVSCGVMVAPVVPFLTDAELERILEAACASGARSASYTLLRLPYELKDLFKDWLTV
                     HYPLKAEHVMSRLREMRGGRENDSEFGSRFRGNGLFADLLEQRFRIACERLGLNREVG
                     ELDVDRFRPPARGGQMGLFE"
     gene            complement(1739..2302)
                     /locus_tag="VFW59_09965"
     CDS             complement(1739..2302)
                     /locus_tag="VFW59_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364580.1"
                     /translation="MNENIKGGTRAHNPDLDWNLLRETILMLELSTGQIEYAMKDSNA
                     SVDVLTESFTSMAGYLRSMSDVLDSLPDDGVAGAKKAEMQQMAVNVTGMVQHSIIAFQ
                     FYDKLTQRLAHVCHSLAAMNDLVGDRSRLYRPSEWVALQEDIRSKYTTAEEHALFEAV
                     MEGVPVQEALDRYMSSMKDKGDDIELF"
     gene            complement(2360..>3041)
                     /locus_tag="VFW59_09970"
     CDS             complement(2360..>3041)
                     /locus_tag="VFW59_09970"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEX5364581.1"
                     /translation="RDAERVFRQDGALCAGVRGWNAGEDGVNDIVSKILSVVVAMSWL
                     VALPASAAEQFDSEQAAQLHCPSDAVVWLNLRSGAIHAKGQPWYGRTQSGAYVCRKEL
                     ANKAGAEATDEKSGWRKIVEDGSRTVYAASSPAAKEGHTVTILSLVDLGKASTLSDGM
                     AFLSWETQYRFDCRKRLSRIEAASMYSGNMGEGEVAGSVIYDAPEWTPIPPGSNGELL
                     WKFACGRK"
BASE COUNT          571 a          995 c          866 g          609 t
ORIGIN      
        1 tggctacttg gatatcgcaa atggtattaa tggcgaggat gtgagaatca aagcaacgca
       61 aatgggcttg attaagcaaa cgtaggttgg gtggagcgca gcgataccca acatcaaaac
      121 cgccggtggc agaccggcaa ctggttacct ttggctccga ctaacctgaa ggtaagcctc
      181 catcgcaact tcgttgtctt gtcttgccaa gaaagccgac aggcttcagt gcaggctcac
      241 atgctcagca tgttatgccg gaactaaagg taaccgaaag aagtcgcccc cggttttccg
      301 ccgcttcgcg gttacctgcg ttgctcgaac ggtcaggtgg ctgcggaact cgcgctacgc
      361 gctcagacag tcctcgccga catcccctga ccgttttccg ctactcggcg gcgcacaggg
      421 gaaaatgcaa aacggttttg tgtgcgctac gcgcacgaat ttaattggct tgattttggt
      481 cgttcatgtc tcgatacggc cttcggccta ctcaacacga acggattgaa acaatgctca
      541 ctcgaacaac cccatctgcc cgcccctcgc aggcggcctg aaacgatcca cgtccagctc
      601 gcccacctcg cgattcaatc ccagccgctc gcaggcgatg cggaaacgct gctccagcag
      661 gtcggcgaac aggccgttgc cgcgaaagcg cgagccgaat tcggagtcgt tctcgcgccc
      721 gccgcgcatc tcgcgcaacc ggctcatcac atgttccgcc ttcagcgggt agtggacagt
      781 cagccagtcc ttgaacaggt ctttcagttc atacggcagc cgcagcaggg tgtagctcgc
      841 gctgcgcgcg ccgctcgcgc aggcggcttc gagtattctt tccagctcgg cgtcggtcag
      901 gaagggcacc accggggcga ccatcacgcc gcaggacacg cccgcctcgt tgagccgctg
      961 catcgtctgc aagcggcggc ggggcgaggc ggcacgcggc tccagcctgc gcgccagttc
     1021 cgcatccagc gtggtgaccg agatgaacac ctgcaccaga ttgtcctgcg ccagctgcgt
     1081 taacaggtcg agatcgcgct cgatcagcgc ggacttggtg acgatggtca gcgggtggcg
     1141 gcactcggcc agcacctcga ggatctgccg cgtgatcttc cagtcgcgct cgatcggctg
     1201 ataggcatcg gtgttgacgc cgagcgcgat cggagaacaa cggtaggaag gtttcgccag
     1261 ctccgcacgc agcagccttg ccgcctccgg cttggcaaac agtctggttt cgaaatccag
     1321 ccccggcgaa agattgaggt aaccgtggct ggggcgcgca tagcaataga tgcagccgtg
     1381 ctcgcaaccg cgataggcgt tgatcgacag ggcgaacggc aggtcgggcg aattctgctg
     1441 ctggatcacg ctcttcgcgc gctcctcggt cacggtggtc ttcagcggcg gcagttcgtc
     1501 gtccaccgtc cagccgtcgt cgacgcgctc gcgggcgacg ctctcgaacc tcccctcgat
     1561 ccgcagcgtc gcgcctcggc ctttgtttac ttcatgcttc atgattgctg aaaagattca
     1621 ctggaagcgt tatcacgcta accatacccc ggaggccgtt cgcattgagc ctgtcgaaat
     1681 gtgaacggcc tcacgcgctt cgacaggctc agcacgaacg gaaaaacaag gcgaacggct
     1741 agaacaactc aatgtcgtcg cccttgtcct tcatgctgct catgtagcga tcgagcgcct
     1801 cctgcaccgg cacgccctcc atcaccgcct cgaacagcgc gtgctcttct gcggtggtgt
     1861 atttgctgcg gatgtcctcc tgcaacgcga cccattcgct cgggcggtac aggcgcgaac
     1921 gatcgccgac caggtcgttc attgccgcca ggctatggca gacatgggcc agtcgctggg
     1981 tcaacttgtc gtagaactgg aaggcgatga tggagtgctg caccatgccg gtgacgttca
     2041 ccgccatctg ctgcatctcg gccttcttcg cgcctgcgac gccatcgtcc ggcaggctgt
     2101 ccagcacatc ggacatcgag cgcagataac ccgccatgct ggtgaaactc tcggtcaaca
     2161 catcgacaga ggcattgctg tccttcatcg catactcgat ctgcccggta gacagttcca
     2221 gcatcaatat cgtctcgcgc agcagattcc agtccagatc cgggttatgc gcccgcgttc
     2281 cgcctttgat gttctcgttc atgtcagctc cttcatccgg ttatcttcgt tcccaacatc
     2341 gcggcgtggc aggcagcgcc tacttcctgc cgcaggcgaa cttccacaac agctcgccgt
     2401 tgctgcccgg cgggatgggc gtccattcgg gcgcatcgta tatcacgctg ccggccacct
     2461 cgccttcgcc catgttgcca gaatacatcg aggccgcctc gatcctggac agtcgtttcc
     2521 tgcaatcgaa ccggtattgc gtctcccacg acaggaacgc catgccatcg ctgagtgtgg
     2581 acgccttccc gagatcgacc aacgacagga tggtcaccgt atggccctcc ttagcggccg
     2641 gggacgaggc ggcatagacg gtgcggctgc cgtcctcgac gattttgcgc cagccggact
     2701 tctcgtccgt cgcctccgcc ccggccttgt tggccagctc cttgcggcag acataggccc
     2761 cggactgagt ccggccatac cagggctgtc ccttggcatg gatcgctccg ctgcgcagat
     2821 tcagccagac cacggcatcg gacgggcaat gcagctgcgc cgcctgttcg ctgtcgaatt
     2881 gctcggcggc gctcgcgggg agtgcgacca gccatgacat ggccaccacg acgctcaaaa
     2941 ttttactcac gatatcgttc actccatctt ctccagcgtt ccatccgcga acaccagcgc
     3001 acagcgcacc gtcttgccgg aatacacgct ctgcatcgcg c
//
LOCUS       DASUIV010000276         4171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_353207, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000276 DASUIV010000000
VERSION     DASUIV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353207"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFW59_09975"
     CDS             complement(<1..307)
                     /locus_tag="VFW59_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family outer membrane porin"
                     /protein_id="HEX5364582.1"
                     /translation="MHPFATRLPSATLACLACCLSPIAGAAGFIEDSKATLQLRNIYF
                     NDDFKDEAGMSAKAAANAQSKKEEWAQGFLLDAQSGYTRGTVGFGLDALGMLGVRLDS
                     "
     gene            complement(417..1721)
                     /locus_tag="VFW59_09980"
     CDS             complement(417..1721)
                     /locus_tag="VFW59_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015137 - citrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015746 - citrate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate:proton symporter"
                     /protein_id="HEX5364583.1"
                     /translation="MLSLLGLSMVVVFTYLIMTKRLSPVVALTVVPIVFALIGGFAPD
                     LGKMMLDGLKTVAPSAALLLFAILFFGIMIDSGLFDPLIRRILQTVNGNPVKIAMGTA
                     VLSLLVALDGDGTTTYMITAAAMLPLYKRIGMNPLILACISMLSLSIMSGMSPWGGPA
                     TRAIAVLGLDASEYFIPMLPTMIGGALWVVFTAYILGRRELKRIGNTHLKSGGNDCYI
                     KAILADSPNKRPKLIWLNLLLVVAVMVALVAGLMNAAVLFMLGFVLALMINYPQLELQ
                     KERILAHSGNALTVVLLVFAAGVFAGIFSGTKMVDSLANSLVEMIPESWGSVFPLVVA
                     ITSMPLTFVLSNDAYYFGMVPILAQAAAAYGIDPVEIARASILGQPVHLMSPLVASTL
                     LLVGMVDRDIGDFQRFTFKWAILTSLVITLLALLTGAIGLVA"
     gene            complement(2040..3140)
                     /locus_tag="VFW59_09985"
     CDS             complement(2040..3140)
                     /locus_tag="VFW59_09985"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HEX5364584.1"
                     /translation="MMHSIPCMIMRGGTSRGPFIHMSDLPESQEARSQVLLDLMGSGH
                     PLQVDGIGGGNSLTSKVAIVGRSSRPDADVDYLFAQVDVIERRVDFSPNCGNMLSAVG
                     PFAIETGLVAPTYGETRIRVFNLNTQRIIECFVPTPQGRVDYEGDTCISGVPGTAAGI
                     RLSFLDVAGSKTGALLPTGKAVDLIDGIEVTCLDAATPTVLIRARDLGVDGTEDPAVL
                     DANTAMLERLERIRCEAGRLMGMGDVRQSVLPKPILLAPARAADATLCARYFVPQRCH
                     KALAVTGAIALGSAVNVPGSVTQRLVLDTGRAVPGQLLGSVRLEHPSGHLDLSVEHRQ
                     GNPLDIARVSLIRTARKIMDGRLFYRMPDALH"
     gene            3275..4168
                     /locus_tag="VFW59_09990"
     CDS             3275..4168
                     /locus_tag="VFW59_09990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5364585.1"
                     /translation="MHRIEFLDLAAFVRVAESQSFQEAAQALHLSQPALSRRLQKLEE
                     TLGAKLLERTTRRTWLTEIGKDYLPRARRMLEDYESSIQGIRELKTHQKGTVTIACIP
                     TAAFYFLPSVIRVFNDAYPGIRIRILDVSANEGLERVVSGDADFGINMISAQHPDVTF
                     TPLRRDPFVLALRSDHPLAALPEVSWSDLQSVRLITVSRDSGNRMLLDSALSGHGIRL
                     NGFYEVQHLSTSLGLVESGLGVAILPRMAMPDSEHETLCSRELPPPHIERTIGLVRRD
                     NEPLSSTAELFIEMLLRQWRE"
BASE COUNT          833 a         1294 c         1328 g          716 t
ORIGIN      
        1 cggagtccag gcgcacaccg agcatgccca gcgcatcgag tccgaaaccg acggtgcccc
       61 gggtgtaacc ggactgggca tcgagcagga agccctgggc ccactcctcc ttcttcgatt
      121 gcgcgttggc ggcagccttg gcgctcatgc ccgcctcgtc cttgaagtca tcgttgaagt
      181 agatgttgcg cagctgcagc gtggccttgc tgtcttcgat gaatcctgcg gcgcccgcaa
      241 tcggggacag gcagcaggcg aggcaggcaa gggttgccga gggcaggcgc gtggcaaatg
      301 gatgcatgga atcttcacct cttgttgttc tggacgcacc cggccggcag gcgcgcgagg
      361 gcgcgcctga ggcggggttc gaagggggat gtcgaagttg cgctcggcgc agtcggtcag
      421 gcgacgaggc cgatggcgcc ggtcagcagg gccagcaggg tgatgaccag ggaggtcaga
      481 atcgcccact tgaaggtgaa gcgctggaag tcgccgatat cgcggtcgac catgcccacc
      541 agcagcagcg tggacgccac cagcgggctc atcagatgca ccggctgacc gaggatcgac
      601 gcccgggcaa tctccaccgg atcgatgccg taggccgctg cggcctgggc gaggatcggc
      661 accatgccga agtagtaggc gtcattggac agcacgaagg tcagcggcat gctggtgatg
      721 gccaccacca gcggaaagac gctgccccag gactcgggga tcatctccac cagcgaattg
      781 gccagcgaat ccaccatctt ggtaccggaa aagatgccgg cgaacacacc ggcggcgaac
      841 accagcaaca ccacggtcag tgcgttgccg gagtgcgcga ggatgcgctc cttctgcagt
      901 tccagctgcg ggtagttgat catcagcgcc aacacgaaac cgagcatgaa cagcaccgcg
      961 gcgttcatca gccccgccac cagcgccacc atcaccgcga ctaccagcag caggttcagc
     1021 cagatcagct tgggacgctt gttcggactg tccgcgagaa tcgccttgat atagcagtcg
     1081 ttgccaccgc tcttcaggtg cgtattaccg atccgcttga gctcgcggcg gccaaggata
     1141 taggcggtaa ataccaccca gagcgctcca ccgatcatgg tcggcagcat cgggatgaaa
     1201 tactcgctgg catccagccc cagcaccgca atggcgcggg tggccgggcc accccagggg
     1261 ctcatgccgc tcatgatgct cagcgacagc atggagatgc aggccaggat cagcgggttc
     1321 atgccaatgc gcttgtacag cggcagcatg gctgccgcag tgatcatgta ggtggtggtg
     1381 ccatcgccat ccagcgctac cagcagcgac aacacggccg tgcccatggc gatcttcacc
     1441 gggttgccgt tgaccgtctg caggatgcgg cggatcagcg gatcgaacag gccggagtcg
     1501 atcatgatgc cgaagaacag gatggcgaac agcagcaacg cagcggacgg cgcgaccgtc
     1561 ttcagaccgt cgagcatcat cttgccgaga tccggggcaa agccgccgat cagggcgaag
     1621 acgatgggca ccacggtgag ggccacgacc ggggacaggc gtttggtcat gatcaggtag
     1681 gtgaacacga ccaccatgga caaaccgagc agagaaagca tggcgttgga ctcttatatt
     1741 tattggttcc agctccatcg gaacggacag tttccacaca tgaccggagt cactgccgct
     1801 gatgatgaag cccgtaaacg catgggagcg caaccgctcc gtgtatcaac cgagcctgtg
     1861 ccggcccggg tcggaatggg ttggcaggct gcgcaggggc aggcgcacga gaaggcggag
     1921 gcaaagcgca ggcaggaaat gcatggtggc tacccatctt gtgattgttg tctgggcgtc
     1981 tgacgacgcg ctgtcgctgc ggctgtcccg tgcgaggatg gccgcgtggg ccgggcgctt
     2041 cagtgcagag cgtccggcat ccgatagaag agacggccgt ccatgatctt gcgggcagtg
     2101 cggatgagcg aaacccgggc gatatccagc gggttgccct ggcggtgctc gacggacagg
     2161 tccaggtggc ccgagggatg ctcgaggcgc acactgccca gcaactgccc tggcactgcc
     2221 ctgcccgtat ccaggaccag gcgctgagtg acgctgcccg gcacgttcac cgcggatccc
     2281 agggcgatgg caccggtaac ggccagagcc ttgtggcagc gctgcggcac gaagtagcgc
     2341 gcgcagagcg tcgcatctgc tgcgcgggcg ggcgccagga gaatcggctt gggcagcacc
     2401 gactgtctta catcgcccat gcccatcagt cgcccggcct cgcaacgaat gcgctccagg
     2461 cgctccagca tggccgtgtt ggcgtcgaga acggcggggt cttcggttcc gtccacgccc
     2521 agatcgcggg cacggatcag cacggtgggc gttgcggcat ccaggcaggt cacttcgatt
     2581 ccgtcgatca gatcgacagc cttgccggtc ggcagcaggg cgccggtctt ggagccagcc
     2641 acatccagaa agctaaggcg gatgccggcg gcggtaccgg gaacgccgct gatgcaggtg
     2701 tcgccttcgt agtcaacccg cccctggggg gtcggcacga agcattcgat gatgcgctgg
     2761 gtattgaggt tgaacacccg gatgcgggtt tccccgtaag tgggcgcgac caggccggtc
     2821 tcgatggcga acggaccgac agccgacagc atgttgccgc agttgggact gaagtccacg
     2881 cgccgctcga tcacgtcgac ctgagcgaac aggtagtcga cgtccgcatc cggccggctg
     2941 gagcgaccga caatcgccac cttgctggtg agcgaattgc cgccgccgat accgtcgacc
     3001 tgcagtgggt gaccagagcc catcaggtcg agcaagacct ggctgcgagc ctcctgtgat
     3061 tcgggcagat cggacatgtg aatgaagggg ccccgagagg tgccgccgcg catgatcatg
     3121 caaggaatgc tgtgcatcat ttgctctacc ccgtgactgc tttaacgatg tgcaaagctt
     3181 gtcagcgcgg atttgatgct tcaaatgcaa atatttatgt gcattaatgc ggtaatcgga
     3241 ttaatgcaca gatcaaatct gatcaggtgg tttcatgcat agaatagaat tcctagacct
     3301 tgccgcattc gtccgcgtgg ccgagtcaca atccttccag gaagccgcac aggccctgca
     3361 cctgtcccag cctgcccttt cccgccgtct gcagaagctg gaagaaaccc tcggcgccaa
     3421 gctgctggaa cgtaccaccc ggcgtacctg gctgaccgag atcggcaagg attacctgcc
     3481 ccgcgcccgt cgaatgctgg aggactacga gtcgtcgatc cagggcatac gcgaactcaa
     3541 gacgcaccag aaaggcacgg tcaccatcgc ctgcatcccc acggcggcct tctacttcct
     3601 gcccagcgtc atccgcgtgt tcaacgatgc ctatccgggg atccgtatcc gcatcctcga
     3661 cgtcagcgcc aatgaaggac tggagcgcgt ggtaagcggc gatgccgact tcggtatcaa
     3721 catgatcagc gcacagcatc cggatgtgac attcaccccc ctgcgccgcg atcctttcgt
     3781 gctcgccctg cgcagcgacc accctctagc ggcacttccg gaggtatcct ggagtgacct
     3841 gcagagcgtt cgcctgatca ccgtcagccg agatagcggt aaccgcatgc tgctggacag
     3901 cgccctttcc ggccatggaa tccggctcaa tggcttctac gaagtgcagc acctctcgac
     3961 ctccctgggg ttggtcgaaa gcggactcgg cgtagccatc ctgcctcgta tggccatgcc
     4021 cgattctgaa cacgaaaccc tctgctcgcg ggagctgccc cctccccata tcgagcgcac
     4081 cattggcctg gtgcgtcgcg acaacgagcc gttgtcgagc accgccgagc ttttcataga
     4141 aatgctgtta cgacagtggc gagagtagcc a
//
LOCUS       DASUIV010000277         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_166023, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000277 DASUIV010000000
VERSION     DASUIV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166023"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..3391)
                     /locus_tag="VFW59_09995"
     CDS             complement(59..3391)
                     /locus_tag="VFW59_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5364586.1"
                     /translation="MSEPLDNAIALSPAHSAVVEACAGSGKTWLLVSRIVRLLLDGVP
                     PGEILAITFTRRAAQEMQARLRDWLYELASKDDDQVREFLRLREVSDGDIERLLPRAR
                     SLYQQFLLTQPGITISTFHGWFMQVLQRAPLTAGMAGGVQLVEQTSALRDEAWKTLLD
                     RLHGHPESPEAQGMLMLFAELGLSNTEQLLFNFLAKRSEWWAYSAGQAEPLAFALDSL
                     RDELDVDMAFDPVADWGMCGNNEEVLFAFVRQFAHNGTDTQQNKASELERVWTDAKPE
                     ARFDELKPLLFTQAGEVRSFKPTKKQNAEAFLLTRESLFATLQQVRDTLAEQQAYRLN
                     EAALRCGVALLESYQNLKQRQQMLDFGDLEWRVCQLLNDSDHAEYLQYKLDSRYSHVL
                     LDEFQDTNPLQWQILQSWFSAADAVDSRPCVFVVGDPKQSIYRFRRADARLFGVVREF
                     LQKHYAAQHLTQNMTRRNAPAVLAAVNGVFDGLPEYEGFETHGAYQQSLPGYVEALPL
                     AKLEEVPALDPQSSILVLRDPLTEPYDEREESAREQEAQQFAAKIGEIVGHWHVHDEG
                     EIRPARYSDIMVLVRRRTYLRVYEEALRAQGIPCLTSRRGGLLETLEALDIQALLTFL
                     ITPFADLQLAHALRTPIFSCSDDDLMALAEIGIEDRGSRIEGRGVEIEEGVASEILPP
                     SPVLSTRSSWWPRLQRLVASGTASMHLQRAHRLLQGWLKLADKLPVHDLLDRIYFEGD
                     VQQRYAAAVSEALRATVQANLQAFMEIALNVDAGRYPSLPGFLRELAELRRADDSESP
                     NEGRVAHAGNALRIYTVHESKGLEAPIVWLLDTNAKPPADKGYDVLLDWPTASQRPAH
                     FSLYGDKGSRGAVREHYFEQDAQLARREDLNLLYVAMTRAKQALLVSGNGERIEGLWY
                     DRIANALAPDAGNPLPGERLVSGKVDRPVANVEVDERLTQALPTGQRSAPPSARQQRG
                     IWLHALLQHLLPPSEAVDEIELRQRCAIPASEFESMLDQAQALLAQPQLARFFDPLHY
                     RSACNEMPYVDAQGELKRIDRLVEFDDEVWVLDYKLGESSGTARHRAQMEEYRSAMQS
                     VYSGKTVRCALVFADGTLEKME"
BASE COUNT          586 a         1137 c         1045 g          637 t
ORIGIN      
        1 agtgcgacca gccatgacat ggccaccacg acgctcaaaa ttttactcac gatatcgttc
       61 actccatctt ctccagcgtt ccatccgcga acaccagcgc acagcgcacc gtcttgccgg
      121 aatacacgct ctgcatcgcg ctgcgatatt cctccatctg tgcgcggtgg cgcgcggtgc
      181 cgctgctctc gccgagcttg tagtccagca cccacacctc gtcgtcaaac tcgaccagcc
      241 ggtcgatgcg cttcagttcg ccctgcgcgt cgacgtaggg catctcgttg caggcgctgc
      301 ggtaatgcag cggatcgaag aagcgcgcca gttgcggttg ggcgagcagg gcctgcgcct
      361 gatccagcat cgattcgaat tcggatgcag gaatcgcgca gcgctggcgc aactcgatct
      421 cgtccacagc ttcacttggc ggcaacagat gttgcagcag cgcatgcagc cagatgccgc
      481 gctgctgtct ggcgctcggc ggcgcgctgc gttgaccggt cggcaaggct tgcgtcagcc
      541 gctcatccac ttcgacgttc gcaactggcc tgtcaacctt gccggacacc aggcgctcgc
      601 ccggcaaagg atttccggca tccggtgcaa gcgcattggc gatgcggtcg taccacaagc
      661 cttcgatgcg ttcgccgttg ccgctgacca gcagcgcctg tttggcgcgc gtcatcgcga
      721 catacagcag attcaagtct tcgcgccgtg cgagctgtgc gtcctgctcg aagtaatgct
      781 cgcgcacggc gccccggctg cccttgtcgc cgtacagcga aaaatgcgcc ggacgctggc
      841 tggcggtcgg ccagtccagc agcacgtcgt agcccttgtc ggcgggcggc ttggcgttcg
      901 tgtccagcag ccagacgatg ggcgcttcca gccccttgga ctcgtgcacg gtgtagatgc
      961 gcaacgcatt cccggcatgg gccacgcggc cctcgttggg cgactcgctg tcgtcggcac
     1021 ggcgcagctc ggccagctcg cgcagaaaac cgggcaggct gggatagcgt ccggcatcga
     1081 cgttcagcgc gatctccatg aaggcctgca gattcgcctg caccgtggcg cgcaacgcct
     1141 cggatacggc tgcggcatag cgctgctgca cgtcgccttc gaagtagatg cgatccagca
     1201 gatcgtgcac cggcaacttg tcggcgagtt tcagccagcc ctgcagcagg cgatgcgcgc
     1261 gctgcagatg catagatgct gtgccgcttg cgacgaggcg ctgtaaccga ggccaccagg
     1321 aggaccgggt gctgaggact ggggacggag gaagaatctc gctcgcaact ccctcctcaa
     1381 tctcaacgcc tcgtccctcg atcctcgatc ctcgatcctc gatcccgatt tctgccagcg
     1441 ccatcagatc gtcgtcgctg caggagaaga tcggcgtgcg caacgcgtgc gccagctgca
     1501 ggtcggcgaa gggcgtgatc aggaaagtca gcagcgcctg aatatccaag gcctccagcg
     1561 tctccagcag accgccgcgc cgcgaggtca gacagggaat gccctgcgcg cgcaacgctt
     1621 cctcgtacac ccgcagatac gtgcggcggc gcaccagcac catgatgtcg gagtaacgcg
     1681 cggggcggat ctcgccctca tcgtgcacat gccaatgccc gacgatctcg ccgatctttg
     1741 cggcgaattg ctgcgcctcc tgttcgcgcg cgctctcctc gcgctcgtcg taaggctcgg
     1801 tcagcggatc gcgcagaacc aggattgagg attgaggatc gagggccgga acttcttcca
     1861 gctttgccag cggtagcgcc tcgacgtatc ccggcaggct ctgctggtag gcgccgtgcg
     1921 tctcgaatcc ttcgtattcc ggcaaaccgt cgaacacgcc gttcaccgcc gccagcaccg
     1981 caggcgcgtt gcggcgcgtc atgttctgcg tcagatgctg cgcggcgtaa tgcttctgca
     2041 ggaactctcg caccacgccg aacagccttg catcggcacg gcggaagcgg tagatggatt
     2101 gcttgggatc gcccaccacg aacacgcagg gtcgcgaatc gaccgcatcg gccgcagaaa
     2161 accacgactg caatatctgc cattgcagcg ggttggtgtc ctgaaactcg tccagcagca
     2221 catggctgta acggctgtcc agcttgtatt gcaggtattc ggcgtggtcg ctgtcgttga
     2281 gcagctggca cacgcgccat tccagatcgc cgaagtccag catctgctgg cgctgcttca
     2341 ggttctgata gctctcgagc agcgcgacgc cgcagcgcag tgccgcttcg ttgaggcgat
     2401 aggcctgctg ctcggccagc gtgtcgcgca cttgctgcag cgtggcaaac agcgattcgc
     2461 gcgtcaacag gaatgcctcc gcattctgtt ttttggttgg tttgaaactg cgtacctcgc
     2521 ctgcctgggt aaacagcagt ggtttcagtt cgtcgaagcg cgcctcgggc ttcgcatccg
     2581 tccagacgcg ctccagttcg gaagccttgt tctgctgcgt gtcggtgccg ttatgcgcga
     2641 actgacgcac gaaggcgaac agcacttcct cgttgtttcc gcacatgccc cagtccgcaa
     2701 ccggatcgaa cgccatgtcc acatcgagtt cgtcgcgcag gctgtccagc gcgaacgcca
     2761 gcggctccgc ctgccctgca ctgtaggccc accactcgct gcgcttggcg aggaagttga
     2821 acagcagctg ctcggtgttg gagagcccca gctcagcgaa cagcatcagc atgccctgcg
     2881 cctcgggcga ttcgggatgt ccgtgcagcc tgtcgagcag cgtcttccag gcctcgtcgc
     2941 gcagcgcgga ggtctgctcc accagttgca cgccccccgc catgcctgcg gtgagcggtg
     3001 cgcgttgcaa cacctgcatg aaccagccgt ggaaggtgct gatggtgatg cccggctgcg
     3061 tcagcaggaa ctgctggtac aggctgcgcg cgcgcggcag cagacgttcg atgtcgccat
     3121 cgctcacttc ccgcaggcgc aggaactcgc gcacctgatc gtcgtccttt gaagccagct
     3181 cgtacagcca gtcgcgcagc cgcgcctgca tctcctgagc tgcgcggcgt gtgaaggtga
     3241 tcgcgagaat ctcgccgggc ggcacgccgt cgagcagcag acgcacgatg cgcgacacca
     3301 gcaaccaggt cttgccgctg ccggcgcagg cttccaccac cgccgagtgg gcgggcgaca
     3361 gcgcgatggc attatccagc ggctcactca tgccacatcc ccttg
//
LOCUS       DASUIV010000278         7135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1190456, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000278 DASUIV010000000
VERSION     DASUIV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190456"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFW59_10000"
     CDS             <1..295
                     /locus_tag="VFW59_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364587.1"
                     /translation="AITQMDEVTQQNAALVEQAAAAAESLEEQAQNLAVAVATFRIDA
                     SSGSAVAVRRAPAATPARKATPAKAPAKAPARAAKAPAKASAKPADDEEWEEF"
     gene            514..3279
                     /locus_tag="VFW59_10005"
     CDS             514..3279
                     /locus_tag="VFW59_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5364588.1"
                     /translation="MPVTNKEYAVTEQDSIVSKTDLKGLITYVNEDFLRVSGFVESEL
                     IGISHNIVRHPDMPSEAFADLWAALKAGRPWTGLVKNRCKNGDFYWVVANVTPIFENG
                     QHVGYMSVRSKPTQEQVSSASAAYALFRNGQAGGLRIKDGAVVKPGLLGKLNLLKNAS
                     VKTRMVIVLSLLSALMIGIGSMGLLGMAKSNEGLKAVYLDRTVPLEQVAEISDLMNEN
                     IQLLYMASEHDPRREESRGQGHVITEHTDKIDKNIASITGIWERYVASQMTGEEKRLA
                     DEYAALRKSFVKDGLLVAQALYLEGRYAEANGVMVKVVAPKGALALAKADELKQLQSR
                     IAKEEFEAAELRYESAKAQVMGLIILGSLLALWALITVLYGVIRPINTVVALLQQIAQ
                     GKYNNTFGIDRQDEIGKLLEALKSMQIKLGFDVADTLRVSNENLRIKIGLDNVSTSVM
                     IADTERKIIYMNKAVSELMQVAEADIRKAIPSFKASELLGGSIDGFHKNPAHQKSMLE
                     SLNSTHRTEIKIGGRTFALAANPVINERGTRLGAALEWKDRTAEVAVEKEVADIVNGA
                     VQGDFTNRIDMQGKEGFFKQLGEGVNLLMETSDRGLQEVVRMLGALARGDLTDRITNE
                     YSGTFGQLKDDANATAERLKEIIGQIKDATDTINTASKEIAAGNSDLSQRTEEQASSL
                     EETASSMEELTSTVKQNAENARQANQLALGASDVASKGGTVVRDVVVTMSSINESSRK
                     IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLAQRSAAAAKEIK
                     TLIDDSVEKVEGGSKLVALAGDTMEEIVTSIKRVTDIMSEITAASSEQSQGIEQVNTA
                     ITQMDEVTQQNAALVEEAAAAAEALEEQAQNLAVAVGTFKVDGGDSLNRRVPAGKLAV
                     KVATKSR"
     gene            3434..4309
                     /locus_tag="VFW59_10010"
     CDS             3434..4309
                     /locus_tag="VFW59_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX5364589.1"
                     /translation="MTDNSQSSSGRDREFTFTNRDFERVRKLIYDHAGISLNPSKQDM
                     VYSRLARRLRATGIDNFDDYLALLEHNDSAEWEAFVNSLTTNLTSFFRESHHFPLLAE
                     HVLKHKHKKHIELWCSAASTGEEPYSMAMAVVDAFGSFTPPVHILATDLDTNVLAKAE
                     AGVYPLERVEKLSPDLVKRFFLKGSGTKDGFVRVRPELRALITFRQLNLLSADWPVRG
                     PLDAIFCRNVMIYFDKATQLKILERFAPLLQPDGLLFAGHSESFHNAGHLFALRGKTV
                     YELAKQKAHGLPLSK"
     gene            4349..4963
                     /gene="cheD"
                     /locus_tag="VFW59_10015"
     CDS             4349..4963
                     /gene="cheD"
                     /locus_tag="VFW59_10015"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HEX5364590.1"
                     /translation="MTVHGYEEILAPNVYFDREHDIEAAKILPGEYYATGRDMVLVTV
                     LGSCVCACLRDRKSGIGGMNHFMLPDAGGDQGGPLADSARYGAYAMEILINQLLKIGA
                     KRENLEAKVFGGGAVLRGFTIANVGERNAEFVLDFLRTESIPVLAQDLLDIYPRKVYF
                     FPATGLVRVKKLKQVHNNTVVDREAEYKMRLRYSKVEGDVELFG"
     gene            5028..6125
                     /locus_tag="VFW59_10020"
     CDS             5028..6125
                     /locus_tag="VFW59_10020"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX5364591.1"
                     /translation="MENGKIKVLVVDDSALIRSVMKEIIDREKDMVCVGAAPDPLVAR
                     EMIKSLNPDVLTLDVEMPKMDGLEFLERLMRLRPMPVVMVSTLTERGSDITFRALELG
                     AVDFVSKPKMDIARGMTEYAIEITDKIRAAAQSRVRTKRAVTPMVVDEKFSADAILSS
                     SVAGRYASTEKLIIIGASTGGTEAIKEVLTRLPADVPGILVTQHMPENFTKSFADRLN
                     SLCKISVKEAEHNERILPGHAYIAPGHSHLLLKRSGANYMTELNQGPPVNRHRPSVDV
                     LFRSAANVAGANAMGIILTGMGKDGAQGLLEMKHAGSFTIAQDEASCVVFGMPKEAIA
                     VGGASEVLPLQNVARRTLEYLAANSGKSNRV"
     gene            6150..6464
                     /locus_tag="VFW59_10025"
     CDS             6150..6464
                     /locus_tag="VFW59_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5364592.1"
                     /translation="MAISIQIRDNVGRITMEGRFDFQVHREFKEAYTPLVDNAAVREI
                     EVEMSKVDYLDSSALGMLMLLNERAKGANKTVSLLNTSGVVSQVLEVANFSKIFNIRN
                     VA"
     gene            6595..>7135
                     /locus_tag="VFW59_10030"
     CDS             6595..>7135
                     /locus_tag="VFW59_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5364593.1"
                     /translation="MLVSKTDLKGVITYCNQAFLKISGYDDRELIGASHNLVRHPDVP
                     SEIFADLWATIKAGNPWNGIVKNRCKNGDYYWVEANVTPLLENGKMVGYVSLRYRPSA
                     EQIAAAKNAYQAVREGLPVMQLSSPDLGYIVQLQQRLAEKFVVLEKYQERSEEELRLG
                     SFIMERMLKIDDSLNDLIRR"
BASE COUNT         1623 a         1739 c         2275 g         1498 t
ORIGIN      
        1 cgcgatcacc cagatggacg aggtgacgca gcagaatgcg gcgctggtcg agcaagcggc
       61 tgcggcggca gaatcgctgg aagagcaggc acagaacctg gcggtggcgg tggcgacctt
      121 caggattgac gccagctccg gcagcgcagt agcggtacgc cgtgctcctg cagcgacacc
      181 cgcccgcaag gctactccgg ccaaagcgcc agccaaggca cctgcaaggg cagccaaggc
      241 tcctgcgaag gcctcggcga agcctgcgga cgacgaggag tgggaagagt tctaaggtga
      301 gggaggcctc gtgcctccct ctgcggtgac tcggaaaaac agattcggta tgagcactcc
      361 gcctacgctc agaactggtg tggcgaagcg tctcataccg aaattgtttt atggtgaccg
      421 atgcctgagc tttatttcat gcggtcgaga tagatttatt actgaataca acctagaacg
      481 ctcaatcgga aatatacatt catgaaaata aatatgcctg tcacgaataa agaatatgcg
      541 gtgacggagc aggattccat cgtctccaag accgacctga agggtctcat cacctatgtg
      601 aacgaggact ttttgcgtgt cagtggcttc gtggaatcgg agttaatcgg gatctcgcac
      661 aacatcgtgc gccatccgga catgccatcc gaagctttcg ccgatctgtg ggccgcattg
      721 aaggctggtc gaccttggac ggggctcgta aaaaatcgct gcaagaacgg cgacttctat
      781 tgggtagtcg ccaatgtcac gccaatcttc gagaacggac agcatgtcgg ttacatgtcg
      841 gtgcgcagca aaccgaccca ggagcaggtc agcagtgcct cagctgccta tgcgttattc
      901 cgtaacggtc aggcaggtgg cttgcgtatc aaggatggcg cggtggtcaa acccggcctc
      961 ttgggcaaac taaacctgct gaagaatgca tccgttaaaa cgcgcatggt gattgtgctt
     1021 agcctcttgt cagcgttgat gatcgggatc ggcagcatgg ggttgctcgg tatggccaag
     1081 tcgaacgaag gtctgaaggc tgtctatctg gatcgcacgg ttcccctgga gcaggttgcc
     1141 gagatatcgg atttgatgaa tgaaaatatt cagctactgt acatggccag tgagcatgac
     1201 ccgcgccgag aagagagtcg agggcagggg cacgtgatca cggaacacac cgacaagatc
     1261 gacaagaata tcgctagcat caccggaata tgggagcgtt atgttgcatc ccagatgacg
     1321 ggtgaggaaa aacgcctagc ggatgaatat gccgcattgc gcaaatcgtt tgtgaaggat
     1381 gggctgctcg ttgcccaggc gctctatctg gaaggtcgat atgccgaagc caatggagta
     1441 atggtcaagg tggtagctcc aaaaggagct ttagctttag ccaaggctga tgagttaaaa
     1501 cagttgcagt ctcgtatcgc caaggaggag tttgaagcgg ctgagttacg gtatgaatca
     1561 gcaaaggctc aagtgatggg attgatcatt ctgggttcgc tgctggcctt gtgggccctg
     1621 attaccgtgt tgtacggagt cattcgtccg atcaatacgg ttgttgctct gctgcagcag
     1681 atcgcacagg gtaaatacaa caacacgttc ggtatcgatc gtcaggatga gatcggcaag
     1741 ttgctcgaag cccttaaatc gatgcagatc aagctgggtt tcgatgtggc ggacacgcta
     1801 cgtgtttcca acgagaacct gcgcatcaag atcggcctgg ataacgtctc gaccagtgtg
     1861 atgatcgccg ataccgagcg caagatcatc tatatgaaca aggcggtcag tgagttgatg
     1921 caggttgcag aggccgatat ccgcaaggcg ataccgagct tcaaggcgtc ggaattgctg
     1981 ggcggaagta tcgatggttt ccacaagaac ccggcgcatc agaaatctat gctggaaagt
     2041 ctgaacagca cgcaccgtac cgagatcaaa ataggcgggc gtactttcgc gttggcggcc
     2101 aatccggtca tcaacgaacg tggcacgcgt cttggcgctg cgctggaatg gaaggatagg
     2161 acagcagagg tggcggttga aaaagaggtg gccgacatcg tgaacggtgc ggtgcagggt
     2221 gactttacca atcgtatcga catgcagggc aaggaaggct tcttcaagca gttgggcgaa
     2281 ggtgtcaatc tgctgatgga gacttcggat cgcggtttgc aggaggtggt gcgcatgctg
     2341 ggggcgttgg cgcgaggcga tctgacagac cgtatcacca atgaatacag cggtaccttc
     2401 gggcagctga aggatgatgc caacgcgacg gctgagcgcc tcaaggagat catcggtcag
     2461 atcaaggatg cgaccgacac tatcaacacc gcctccaagg agatcgccgc cggaaacagc
     2521 gacctgtcac agcgcaccga agagcaggcc tccagtctgg aagaaaccgc cagcagcatg
     2581 gaagagctga cctcgaccgt caagcagaat gccgagaacg ccagacaggc caatcaactt
     2641 gccctgggtg catcggacgt ggccagcaag ggcggtacgg tggttcgcga cgtggtcgtg
     2701 accatgagtt cgatcaacga atcctcgcgc aagatcgtcg atatcattag tgttatcgat
     2761 gggatcgcat ttcagaccaa catcctcgcg ttgaacgccg cagtggaagc ggcacgcgcg
     2821 ggcgaacagg ggcgtggttt cgcggtggtc gcgggtgaag tgcgcaatct ggcacaacgc
     2881 tctgccgctg cggcgaaaga gatcaagacg ttaatcgacg attcggtgga gaaggtcgaa
     2941 ggcggcagca agctggtggc gctggctggc gataccatgg aagagatcgt gaccagcatc
     3001 aagcgcgtga ccgacatcat gtccgagatc acggcagcat ccagcgagca gagccaaggt
     3061 atcgagcagg taaacaccgc catcacccag atggacgaag tgacgcagca gaacgcggcg
     3121 ctggttgaag aagcggctgc tgctgccgag gcgctggaag aacaggcgca gaatctggcg
     3181 gtggcggtgg ggaccttcaa ggtggatggg ggcgacagcc tgaaccgtcg tgtgccggca
     3241 ggcaagctgg cggtaaaggt cgcgaccaag tccaggtaga agggcgccgt ggtctagtcg
     3301 gagcgttgat cgatcatcgt gtaggatgcg cgtcgctgcc tcgcctgtcc gattgattgg
     3361 cgcggctgat aagcctccga catggattaa atattgaata ggctgcacag gttgtaaatt
     3421 aaagtgagac gagatgactg ataattctca atccagtagc ggtcgtgatc gcgagttcac
     3481 cttcacgaac cgggatttcg agcgagtgcg caagctgatc tatgatcatg ccggcatttc
     3541 gctcaacccc tccaagcagg acatggtgta cagccgtctg gcgcgacgct tgcgcgcgac
     3601 cgggatcgat aatttcgatg actatctggc tctgctggag cataacgata gcgcggagtg
     3661 ggaggcgttc gtcaattcgc tgaccacgaa tctgacctcg ttttttcgcg agtcgcatca
     3721 ttttccgttg ctggccgagc atgtgctcaa acacaagcac aagaagcata ttgaactgtg
     3781 gtgttcggcc gcatcgacgg gagaagagcc ctattcgatg gcgatggcgg tggtggatgc
     3841 attcggctcg ttcacgccgc ctgtccatat attggcgaca gatctggaca ccaatgtatt
     3901 ggccaaggcc gaggcggggg tgtacccgct ggagcgcgtg gagaagttgt cgcccgatct
     3961 ggtgaaacgc tttttcctca agggttcggg aaccaaggat ggttttgtgc gtgttcgacc
     4021 ggagttgcgc gcgctgatta cctttcggca gctgaatttg ttgagcgcgg attggccggt
     4081 tcgcggcccg ctggacgcca ttttttgccg caatgtgatg atctattttg acaaggccac
     4141 gcagctcaag attctggagc gtttcgctcc gttgttgcag ccggacggct tgttattcgc
     4201 gggccactcc gagagctttc ataatgcggg gcatctgttt gcgctgcgcg gcaagacggt
     4261 gtacgagttg gcgaagcaaa aggctcatgg actgccgctc agtaaatgac atggttgact
     4321 gggtgataag cagggaaaag ggcagcgaat gaccgtacat ggttatgaag agattctggc
     4381 tcccaatgtc tactttgatc gggagcacga catagaggcc gccaagatac tcccgggcga
     4441 gtattacgcg accgggcgcg acatggtgct ggtgacggtg ctgggttcct gtgtgtgcgc
     4501 ctgtctgcgc gatcgcaaga gcggtatcgg cgggatgaac cactttatgt tgccggatgc
     4561 gggcggcgat caaggggggc cgctggccga ttcggccagg tatggcgctt atgcgatgga
     4621 aatcttgatc aatcaactgc tcaagatcgg cgcgaagcgc gagaatctgg aggcgaaggt
     4681 gtttggcggc ggggcggtgc tgcgcggctt tacgattgcc aacgtgggtg agcgcaatgc
     4741 ggaattcgta ctggactttc tgcgtacgga gagcatcccg gtattggcgc aggacttgct
     4801 ggatatctac ccgcgcaagg tgtatttctt cccggcgacg ggattggtgc gggtgaaaaa
     4861 gttgaagcag gttcacaaca atacggtcgt cgatcgcgaa gcggaataca agatgcgctt
     4921 gcgctactcc aaggtcgagg gcgatgtgga gttgttcggt tgattaggca agggcacggc
     4981 gcgccgtgcc cttacggcgg taacaaaatt gtcgggctag agtgaatatg gaaaacggca
     5041 agatcaaggt tctggtggtt gatgactccg cgctgatacg cagcgtgatg aaagaaatta
     5101 tcgatcgcga gaaagacatg gtgtgcgtgg gcgcggcacc cgatccgttg gtggcgcgcg
     5161 agatgatcaa gtcgctgaat cccgatgtgc tgacgctgga tgtggaaatg cccaagatgg
     5221 acgggctgga gtttctggag cgtctgatgc gtctgcgtcc gatgccggtg gtgatggttt
     5281 ccacgctgac cgagcgcggt tccgatatca cgtttcgggc actggagctg ggtgcggtgg
     5341 atttcgtctc caagccgaag atggatattg ctcgcggcat gacggagtat gccatcgaga
     5401 taacggacaa gatccgtgcc gccgcgcagt cacgtgtgcg cacgaagcgg gcggtcaccc
     5461 ccatggtggt ggatgagaaa ttttctgccg atgccatcct ttccagcagt gtggcgggac
     5521 gttacgcttc aaccgagaag ctgatcatca tcggagcttc cacgggcgga accgaggcga
     5581 tcaaggaggt gctgactcga ttgccggcgg atgttcccgg tatactggtg acccagcaca
     5641 tgccggaaaa cttcaccaag tcttttgcgg accggttgaa tagtctgtgc aagatttcag
     5701 tcaaggaggc ggagcacaac gagcgcatct tgccggggca tgcctatatc gcaccggggc
     5761 actcgcatct gctgctcaaa cggagcgggg cgaactacat gaccgagttg aaccaggggc
     5821 cgcctgtcaa ccgtcaccgg ccatcggtgg atgtgctgtt ccgttcggct gccaatgtgg
     5881 cgggtgcgaa tgcgatgggg atcatcttga ccggtatggg caaggatggc gcgcagggtt
     5941 tgctggaaat gaagcatgcc ggatcgttca ctattgcgca ggacgaggca agttgtgtag
     6001 tgttcggtat gcctaaagag gcgatcgcgg tgggcggtgc gagcgaggtg ttgccgttgc
     6061 agaatgtggc gcgacgaacg ctggaatatc tggcggcgaa tagcggcaag agtaaccggg
     6121 tttaataatt aacagagaag gagttgatga tggctatcag tattcaaatt cgtgacaatg
     6181 tcgggcgcat tacgatggag gggcgcttcg attttcaggt gcatcgtgaa ttcaaggagg
     6241 cctacacgcc actcgtcgac aacgccgctg tgcgcgagat cgaggttgag atgtccaagg
     6301 tggactacct ggacagctcg gcgctgggga tgttgatgtt gctgaatgaa cgcgccaaag
     6361 gggcgaataa aacggtctct ttgctgaaca cctcgggtgt ggtgtctcag gtgttggaag
     6421 tggccaactt cagcaagata ttcaatatcc gtaacgttgc ttgatggtga gcagggagtg
     6481 gtgacaggga agtcgtcact cgccattcat cactcttgac tccccctact ccccactttc
     6541 atgcgaataa atcagccagt caccgatatt gagcatcccg ttccggaagg tgtggtgctc
     6601 gtttctaaaa ccgatctcaa gggtgtcatc acctattgca accaggcctt tctcaagata
     6661 tccggatacg acgatcgtga attgattggc gcgtcgcaca atctggtgcg tcatccggat
     6721 gtgccgtcgg agatatttgc cgacctgtgg gcaaccatca aggcgggcaa tccctggaac
     6781 ggtatcgtca agaatcgctg caaaaatggg gattactact gggtcgaggc caacgtcacg
     6841 ccgttgctgg agaacggcaa gatggtcggc tatgtgtcgc tgcgttatcg tcccagcgcg
     6901 gagcagattg ccgcagcgaa aaatgcctat caggccgtgc gcgaggggct gcccgttatg
     6961 cagctgtcca gccccgatct gggttacatc gtgcagctgc agcagcggct ggccgagaag
     7021 ttcgtggtgc tggaaaaata ccaggagcgc tccgaagaag agctgcgtct cggcagcttt
     7081 atcatggagc gcatgctcaa gatagacgac agcctgaacg atttgattcg tcgct
//
LOCUS       DASUIV010000279         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_724865, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000279 DASUIV010000000
VERSION     DASUIV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724865"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFW59_10035"
     CDS             <1..594
                     /locus_tag="VFW59_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX5364594.1"
                     /translation="IAILTFEGFNELDSLIALGVLNRIKRPGWRVTLSCPAPSVTSMN
                     DVTVHAQSTLAEACAADAVIIGSGVKTREIVNNPELMANLKLDPARQLIGAQCSGTLL
                     LAKLGLLGSVPACTDLTTKPWVQEAGIEVLDQPFYAQGNVATAGGCFASQYLAAWIIA
                     RTEGEEAARNALHYVAPVGEKEEYVSRAMKNIAPYLP"
     gene            712..1494
                     /gene="blaOXA"
                     /locus_tag="VFW59_10040"
     CDS             712..1494
                     /gene="blaOXA"
                     /locus_tag="VFW59_10040"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEX5364595.1"
                     /translation="MKRFLMVVILLFASQAYALEWQDSPQVGQLFRDAGVSGTFVLYD
                     VAALRLIGYDQKRADTRFAPASTFKIFNTLVGLASGAVKDVDQILPYGGSPQPVKAWE
                     KDMSLRDAIAISNVAAYQVLARRIGLERMRKEISGMNYGNGEIGTSIDNFWLVGPLAI
                     SAIEQTQLLAQLAEGALPFPKEMQASVREIIRLEQKDNYALYGKTGWGNFPDPGIGWW
                     VGWVQKEGRVYAFALNIDMQRASDAGKRAELGRACLKALGIF"
     gene            complement(1472..2014)
                     /locus_tag="VFW59_10045"
     CDS             complement(1472..2014)
                     /locus_tag="VFW59_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX5364596.1"
                     /translation="MNSPPRFLCDEMLGRLCRYLRAAGYDTLFANEGLQDRVLLQQCH
                     DEGRYFLTQDQLVREHKAARDIALILPQGDIDRLAALVDARFQIDWLSHAFTRCLQDN
                     ALLREADAASAARVPQDARRPGEPLTCCPACGRVYWQGSHYKRMRARLAKWQAAKWQA
                     DGCATETGCLASPLKIYPAL"
     gene            complement(2216..3067)
                     /gene="folD"
                     /locus_tag="VFW59_10050"
     CDS             complement(2216..3067)
                     /gene="folD"
                     /locus_tag="VFW59_10050"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX5364597.1"
                     /translation="MTARIIDGKAIAQEVRAEWKARADALKARGITPGLAVIIVGEDP
                     ASKVYVANKVKACAELGLHSEHIALPADTSEATLLAKIAELNADPKIHGLLVQLPVPK
                     HIDSDKVLNAISPDKDIDGFHPMNVGALATGNMRFAPCTPYGSLILLQKSGVAIEGKH
                     AVVVGRSNIVGKPMALLLLQHNATVTICTSKTVDLAKHTRDADILVVATGRPKMITGD
                     MIKPGAAVIDVGINRMADGKLCGDVDFDSAKKVAGWITPVPGGVGPMTITMLVANAVQ
                     AAERAAK"
     gene            complement(3085..>3475)
                     /locus_tag="VFW59_10055"
     CDS             complement(3085..>3475)
                     /locus_tag="VFW59_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5364598.1"
                     /translation="CNYGYIPHTLSDDGDPVDVLVITPIPLQVGCVVRCRPLGVLKME
                     DESGIDAKLLAVPVDKLTAMYRNIKTYTDLPEISLKQIEHFFAHYKDLEPNKWVKIGG
                     WEDGASACAEIMAGVENYKNAAVKPEF"
BASE COUNT          654 a          989 c         1077 g          755 t
ORIGIN      
        1 atagccatcc ttaccttcga agggttcaat gaactcgact ctcttatcgc cttgggcgtc
       61 ctcaatcgca tcaagaggcc aggctggcga gtcacgctct catgcccggc gccatctgtc
      121 acttcaatga acgacgtaac agtacatgca cagtcaacat tggccgaagc ttgcgcggcg
      181 gatgcggtca tcatcggaag tggtgttaaa acccgggaga tcgtaaacaa cccggagctt
      241 atggcaaatc tcaagttaga ccctgcacgc caactaatcg gagctcaatg ttccggcacg
      301 ctgcttctgg cgaagctcgg tctgcttggt agcgttcccg catgtactga cctcaccacg
      361 aagccttggg tgcaggaggc tggcatcgag gttctcgatc agcctttcta cgctcaaggt
      421 aacgttgcaa ccgcaggagg ctgctttgca tcgcagtatc ttgccgcttg gatcatcgcg
      481 cgcaccgagg gcgaagaggc agcgagaaat gcacttcact atgtcgcgcc ggtcggcgag
      541 aaggaagagt atgtttcccg cgcgatgaag aatattgctc cctatctgcc gtagctccgt
      601 gagcacctac gctcaagcgg gactgcgcaa aagcgcgcag cctccttggg atatcagcca
      661 ataagccatg agctatgccg gggggagtca aactttgcga ggatattttg catgaaacgt
      721 tttttgatgg tcgtcatttt gttgttcgct tctcaggcgt atgcacttga gtggcaggat
      781 agtccgcaag tcggccaact gtttcgtgat gcgggcgtat cgggaacatt tgttctttat
      841 gatgtcgctg cactgcgcct tattggttac gatcagaaac gggcagatac gcgattcgcg
      901 ccggcttcca cgttcaagat tttcaacacc ctggtgggtt tggccagcgg tgcggttaaa
      961 gatgtcgatc aaatcctgcc ctatggcgga agccctcagc ccgtcaaggc atgggaaaaa
     1021 gatatgagct tgcgcgatgc catcgccatc tccaacgtgg ctgcttatca agtgctggcc
     1081 agacgcatcg ggctggaacg tatgcgcaag gagatttccg gaatgaacta cggcaacggt
     1141 gaaatcggga caagcatcga caacttctgg ctggtcggtc cgcttgccat cagcgcaata
     1201 gagcaaacgc agttgctcgc tcaactggcg gagggcgcat tgccatttcc caaagaaatg
     1261 caagcctcgg ttcgggagat catccggctt gagcagaaag ataactatgc gctctatgga
     1321 aaaaccggct ggggtaattt ccccgatccg ggaatcggct ggtgggtcgg ctgggtgcag
     1381 aaggaagggc gcgtgtatgc atttgccttg aatatcgata tgcagcgcgc ttccgatgca
     1441 ggcaagcgtg ccgaactggg cagggcctgc ctcaaagcgc tgggtatatt ttgagcggcg
     1501 aagccaggca tcctgtctcg gtggcgcatc cgtcggcttg ccatttggcg gcttgccatt
     1561 tggcgagccg ggcgcgcatg cgtttgtagt gcgagccttg ccagtacact cttccgcacg
     1621 ctgggcaaca ggtcagcggc tcgccgggtc tgcgggcatc ctgaggcacg cgtgccgctg
     1681 atgccgcatc ggcttcccgc aacagggcgt tgtcttgcag gcaacgcgtg aaggcgtggc
     1741 tgagccagtc gatctggaag cgcgcgtcga ccagggcggc cagccggtcg atgtcgcctt
     1801 gcggcaggat cagtgcgatg tcgcgcgcgg ccttgtgctc gcgcaccagt tgatcctgcg
     1861 tcagaaaata gcgcccctcg tcatggcatt gttgcagcaa cacgcgatcc tgaagtccct
     1921 cattggcgaa cagcgtgtcg tagccggctg cgcgcaagta gcggcacaga cggcccagca
     1981 tttcgtcgca aaggaagcgg ggcggtgaat tcatggcggc atgttatgcc aattccatgc
     2041 gttgctgcgg agaacggcaa gggcttatgc gtgcctcacg gtaatcgtga gttagttgtg
     2101 gcgatggtgc atcgcggggc gatgccggga ggactgagag tcttgacctc taccgccagc
     2161 gtcccggact gcgggacgcg gacgtagagg caagcctgta acgcttgtgg cgtctttact
     2221 ttgccgcgcg ttccgccgct tgcacggcgt tggcgaccag catggtgatg gtcatcgggc
     2281 cgacaccgcc gggaaccggg gtgatccagc ctgcgacttt cttcgccgaa tcgaaatcca
     2341 cgtcgccgca cagcttgccg tcggccatgc ggttgatgcc gacgtcgatg actgccgcgc
     2401 cgggtttgat catgtcgccg gtaatcatct tcggcctgcc ggtcgcaacg accaaaatgt
     2461 cggcatcgcg cgtgtgcttg gcgaggtcaa cggtcttgga ggtgcagatc gtgacggtgg
     2521 cattgtgttg cagcaacagc aacgccatcg gcttgccgac gatgttgctg cggcccacca
     2581 ccaccgcatg cttgccttcg atggcgacgc cgctcttttg cagcaggatc agcgagccgt
     2641 acggcgtgca gggcgcgaag cgcatgttgc cggtggccag cgcgccgacg ttcatcgggt
     2701 ggaagccgtc gatgtccttg tcgggactga tggcgttcag caccttgtcg ctgtcgatgt
     2761 gcttgggcac cggcagttgc accagcaggc cgtgaatctt cgggtctgcg ttcagctcgg
     2821 cgatcttcgc cagcagcgtc gcctctgagg tgtcggcagg cagcgcgatg tgctcggagt
     2881 gcaagccgag ttccgcgcag gccttcacct tgttggcgac atacaccttg gaggccggat
     2941 cttcgccgac gatgatgacc gccagccccg gcgtgatgcc gcgcgccttc agcgcatcgg
     3001 cgcgagcctt ccattcggcg cgcacttcct gcgcgattgc cttaccgtca atgatacgtg
     3061 cggtcatcct ggtctccttg cgaatcagaa ctcgggtttg actgcggcgt tcttgtagtt
     3121 ttccacgccg gccatgatct cggcgcatgc cgatgcaccg tcttcccagc cgccgatctt
     3181 gacccacttg ttgggttcca gatccttgta gtgagcgaag aagtgttcga tctgcttcag
     3241 cgaaatttcg ggcaggtcgg tataggtctt gatgttgcga tacatcgcgg tcagcttgtc
     3301 caccggcaca gccagcagct tggcgtcgat gcccgattca tcttccatct tcagcacgcc
     3361 cagcggacgg caacgcacca cgcaaccgac ttgcagcggg atcggggtga tgaccagcac
     3421 gtccacgggg tcgccgtcgt cggacagggt gtgcgggatg tagccgtagt tgcag
//
LOCUS       DASUIV010000280        12110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_446705, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000280 DASUIV010000000
VERSION     DASUIV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446705"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFW59_10060"
     CDS             complement(<1..286)
                     /locus_tag="VFW59_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEX5364599.1"
                     /translation="MQSFCHWEGDTLVLNILGRPRAKKTVIGKVIGKQLEIHVAEMPV
                     RGRATAHLVRFLAEEFQVPESSITVVFGVYNVNKQLRIAAPKRLPAVIPPP"
     gene            complement(387..3137)
                     /gene="polA"
                     /locus_tag="VFW59_10065"
     CDS             complement(387..3137)
                     /gene="polA"
                     /locus_tag="VFW59_10065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5364600.1"
                     /translation="MNTDNFTKTLLLVDGSSFLYRAFHAMPDMRNREGFPTGAIYGVL
                     NMLRKLRQDYPADYSLCVFDAKGKTFRDDMYPEYKATRPSMPGDLAQQIEPLHQAIIA
                     TGWHILAQPGVEADDVIGTLADHATRDGVRCIVATGDKDLAQLVNEHVTLINTMNNET
                     LDIPGVTAKFGVPPERIIDFLTLTGDQVDNVPGVTKCGPKTAVKWLTQYGTLDNLMQH
                     ADEVSGVVGANLREALEWLPQARQLITVKCDVNLPLPYTAMIAPPADIEQLRSLYERF
                     EFRSWLRELGGTPKSSGSTTEATAPATQDMFADEPTRTDSAHYETILTDAQLDAWLEK
                     LLAAELTCVDTETTGLDVMNAQLVGMSFSVKAHEAAYLPLAHIYPGAPDQLKREHALE
                     KLKPWLESAKHRKLGQNLKYDLHIFANHGIHLAGIHDDTLLQSYVLESHKPHDMDNLA
                     LRHLNVKTVSYAEVAGKGAKQICFDQVDLDTATRYAAEDADITLQLHQTLAPQIAEQS
                     GLEHVYRDIELPTMAVLYIMERNGVLLDSALLQVQSRELGEKLIALEARAFEAAGQPF
                     NLNSPKQLREILFDKLGLPVKKKTPSGDPSTDEEVLQELALDYPLPKILLEYRGMAKL
                     KSTYTDKLPQMVDRATGRVHTSYSQAVAVTGRLASSDPNLQNIPVRTAEGRRIREAFI
                     APTGSRIVSADYSQIELRIMAHLSGDAGLLTAFANNEDIHRATAAEIFMTSREAVTSE
                     QRRYAKVINFGLIYGMSAFGLAKQLGVERAAATAYIDRYFARYPGVKNYMDSTREQAK
                     SQGYVETVFGRRLWLPDINAGNGMKRQGAERAAINAPMQGTAADLIKLAMVAVQHWLQ
                     SENLHSLLIMQVHDELVLEVPENELELVKDMLPKLMCNVAELKVPLLVELGEGMNWDE
                     AH"
     gene            3165..3881
                     /locus_tag="VFW59_10070"
     CDS             3165..3881
                     /locus_tag="VFW59_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5364601.1"
                     /translation="MSIPSKLPVPVVPENWNSKEAWRVFGIMAEFVSATDRLNRIHPA
                     VSIFGSARTKPDQPYYKLAEEISRLLSDAGFAVISGGGPGIMEAANKGAYYGKSPSVG
                     LNIQLPHEQNTNLYQNISHTFQHFFTRKVMFVKFASAYVVMPGGFGTLDELMEALTLV
                     QTGKTRRMPIILVGSKFWAGMVEWFRTALLEEKVISPEDMDLVQVIDDPEAVVSAIFK
                     HYETRGFEPSEAERERQLYL"
     gene            3924..4253
                     /locus_tag="VFW59_10075"
     CDS             3924..4253
                     /locus_tag="VFW59_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEX5364602.1"
                     /translation="MRLPALLLLSGLSFAAFAQPPVPANLEPLPPPPAFEEKDAPGEP
                     EVTITKEAAQTVEEYRANGKLYMIKITPKHGVPYYLVDSKGDGKFARQESADSGLRVP
                     RWVIFKF"
     gene            4255..5193
                     /locus_tag="VFW59_10080"
     CDS             4255..5193
                     /locus_tag="VFW59_10080"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5364603.1"
                     /translation="MAVFTSVSEAELTAWLGDYSLGQLLELQPIASGIENTNYFVTTG
                     NGRFVLTLFEKLTADELPFYLNLMAHLARHGIPCPSPVANRLNQFLGSLNGKPACIAT
                     RLSGKSTTVPNLAQCAAIGAMLGQLHLAGQSFSQIMPNPRGAAWRAATAPQVRPFLDA
                     AQAELLDGEVALHAGSDLSRLPQGVIHADLFRDNVLLEEDRVGGLIDFYFACSDALLY
                     DVAITLNDWCMPEGKLDAARAQAFLRAYHAVRPLQDSERDAWPLMLRLAALRFWLSRL
                     YDLHLPRDGELINAHDPGHFERILRNYIATAQPVWL"
     gene            5205..5957
                     /locus_tag="VFW59_10085"
     CDS             5205..5957
                     /locus_tag="VFW59_10085"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEX5364604.1"
                     /translation="MEIRKLNAARGWTWVKQGYQLIMRNPLLSVSLALTGALAIFAVL
                     HIPLFGPLLAILFMPVLIAGYMRACRALEENEEVELAHLFAGFEKHAVPLISLGGYMM
                     LGLLIVSMVMIFIGGAAFAKLLESFQASNDPQLLIAALGSAGSGVAFTLLVGFSLVFV
                     LMLALQYAPMLVFFSDVKPLAALRASLAGSLRNIIAYSVYSLIMQVVALVLGVLPYGI
                     GMIALLPLGFTSLYVSYRNIFPFPGEIPPAAE"
     gene            6055..6546
                     /locus_tag="VFW59_10090"
     CDS             6055..6546
                     /locus_tag="VFW59_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364605.1"
                     /translation="MQFQASIEEVKRAQAPHNLFISGLFLFNLLMTPAIIALKVGLIG
                     VLIPLFCSGALLGYIYLRSRRTTSWFVDAHWRLSFRHGKWLMLGYAISASLILLAWLL
                     SLTAHEASMKHIMLTALTRIAVIPTLIAVMVTLVLEFGAAAQAVKSEVPDGMVAKFPP
                     PAA"
     gene            complement(6543..7073)
                     /locus_tag="VFW59_10095"
     CDS             complement(6543..7073)
                     /locus_tag="VFW59_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HEX5364606.1"
                     /translation="MRSSPPSDPALDERLRLIVDSYRRLTGKELLDPQAATFVGAASA
                     AMASLHDGGIAAEAAPTKQALWEAPRAIVAHGTEDDPVFFYGNRLALELFDMDFAAFT
                     RLPSRYSAEPLERAERARLLERVTQRGYVDDYAGVRIGASGKRFTINAATVWNLLDEA
                     GKLHGQAATFSDWQPL"
     gene            complement(7051..9216)
                     /locus_tag="VFW59_10100"
     CDS             complement(7051..9216)
                     /locus_tag="VFW59_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5364607.1"
                     /translation="MNSSLLSGLNPEQRAAVELPAQSALILAGAGSGKTRVLTTRIAW
                     LISTGQVSPHGLLAVTFTNKAAKEMVTRLSAMLPINTRGMWIGTFHGLCNRMLRAHHR
                     EANLPQTFQILDSGDQLSAIKRVMKQLNVDDEKYPPREMQNFISGSKEQGLRAHEVEA
                     YDPYTRRKVEVYAEYDAQCQREGVVDFSELLLRCYELLSRNAELREHYQDRFKHILVD
                     EFQDTNPLQYNWLKLLAGRSNALFAVGDDDQSIYAFRGANVGNMQDLLRDFHVANVIK
                     LEQNYRSHGNILDAANALICNNRNRLGKNLWTAESGGEQLRAYEAATDVDEAAFIVDE
                     IKQLQREGIHLKEIALLYRSNAQSRVLEHALVSAGLSYRVYGGLRFFERQEIKHALAY
                     LRLMENTDDDNALLRIINFPTRGIGARSIEQLQDAARQNNTALWDAAARAGGKVSAFV
                     ALIESLRSATKDLPLPEIIDHVLTHSGLVDHFKNETGAKKREAEERLENLNELINSAT
                     MFVHENEDDSLTSFLTHASLEAGEHQAGDQDDALHLMTVHAAKGLEFHTVFITGLEDG
                     LFPHQNSQNVDGGLDEERRLMYVAITRARRQLYLTFAQRRMLHGKASYSTASSFLNEL
                     PEELLRWLSPRFVTAKRFDSYLDSPSSSAPSFGGGAAPARANDESAWRIGQRVFHQKF
                     GEGVITGVEGTGTDGRVQVNFKHAGSKWLSLEYAKLTAV"
     gene            complement(9280..10179)
                     /locus_tag="VFW59_10105"
     CDS             complement(9280..10179)
                     /locus_tag="VFW59_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5364608.1"
                     /translation="MQLVSPRTVHVLRHPLSFTLQVLKAFRENQGLLLAGAVAYYALL
                     SIVPMVILMVIALSQFIDQAALLETVRWALRYVAPGENRTVMTELKAFLEHGGVIGWL
                     LLITLLFFSSLGFRVLESAMSVIFRHRMQKLKRNFFVSLLLPFGYITFICTLLFTGAI
                     LIADLVALGSGNVALFGQSWSLDTFSHALIYGGGLIGEVLLVSALYFFMPVGRLPVHH
                     ALIGGVTAALLWEVVRFLLRWYFGSLSQVGEVYGSLTTTIVLLISLEVAATLLLLGAQ
                     VIAEYERIEVGEATTLHMSMRRK"
     gene            10377..10583
                     /locus_tag="VFW59_10110"
     CDS             10377..10583
                     /locus_tag="VFW59_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX5364609.1"
                     /translation="MNDERLIDIETKIAYQEDLIEELNKTIYQQQKRLERLEAVCQTL
                     AGHIESLNAAKNDGMPANERPPHY"
     gene            10593..11045
                     /locus_tag="VFW59_10115"
     CDS             10593..11045
                     /locus_tag="VFW59_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5364610.1"
                     /translation="MLNRLNDFLATIITPAEGRPEHTLQLATAVLLVEVMRSDAESTD
                     EEQATILDILKQRFQLADAEVAQLTELAHRTAKSATDLHQFTSLINRELAAPEKLRII
                     EYMWQVAYADRQISAHENHLMRRMAELLHIGHGDYIAAKMRAKPAELN"
     gene            complement(11100..>12110)
                     /locus_tag="VFW59_10120"
     CDS             complement(11100..>12110)
                     /locus_tag="VFW59_10120"
                     /inference="COORDINATES: protein motif:HMM:NF023295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HEX5364611.1"
                     /translation="KALKVYTESWGKGMPTVDASEALAKLQEYLEVARDILSEFDYSV
                     YRTQATTLLLPAANFVLGLEDGKKRWFDVVLKITKAFSLCGTLGDAMAMREEIAFFQA
                     IKAVIAKATETDKKLTEEHKNAVLKQILDNAVVAEGVEDIFKLAGLERPDIGILSDGF
                     LEEVRQLPQRNFAVELLQKLLNDEIRSRMHTNVLLEKKFSERLLAALNRYRSRAIESA
                     QVIEELIAMAREFREAAKRGDELGLNESELAFYDALADNESAVRELGDEILKQIAHEL
                     TDKLRNSTTVDWQKRDSVRARLRNLVRITLRRFKYPPDLQEAAIQLVLEQADRLSADW
                     AN"
BASE COUNT         2170 a         3745 c         3750 g         2445 t
ORIGIN      
        1 ccggcggcgg gatcacggcg ggcaaccgct taggcgcggc gatgcgcagt tgtttgttga
       61 cgttgtacac cccgaacacc acggtgatcg agctctcggg aacctgaaac tcctccgcga
      121 ggaaccgcac cagatgcgcc gtggcgcgcc cgcgcacagg catctcggcg acatgaatct
      181 ccagctgttt gccgatcacc ttgccgatca cggtcttctt cgcgcgcgga cggccgagga
      241 tgttcagcac cagcgtgtcg ccttcccaat gacagaacga ttgcattatt tctccagtga
      301 tgtgcttgct tttgtaggag cggcttcagc cgcgattgct cccttcatcg cggctgaagc
      361 cgctcccacg aagaagacag ggccctttag tgcgcctcgt cccagttcat tccctcgccc
      421 agttccacca gcagcggcac cttgagttcg gccacgttgc acatcagctt gggcagcatg
      481 tctttcacca gttccagttc gttctccgga acttccagca ccagttcgtc gtgtacctgc
      541 atgatcagca ggctgtgcag gttttcggat tgcagccagt gctgcaccgc gaccatcgcg
      601 agtttgatca ggtcggcggc ggtgccctgc atcggcgcgt tgatcgcggc gcgttcggcg
      661 ccctgacgct tcatgccgtt gccggcattg atgtcgggca gccacaggcg gcggccgaac
      721 acggtctcga catagccctg agatttggcc tgttcgcgcg tgctgtccat gtagttcttc
      781 acgccgggat agcgcgcaaa gtagcggtcg atgtaggcgg tcgcggcggc ccgttcgacg
      841 ccgagctgtt tggccagacc gaaggcggac atgccgtaga tcaggccgaa gttgatgacc
      901 ttggcgtagc ggcgctgttc gctggtcact gcctcgcgcg aagtcatgaa gatctcggcg
      961 gcggtggcgc ggtggatgtc ctcgttgttg gcgaaggcgg tcagcaggcc tgcatcgcct
     1021 gacaggtggg ccatgatgcg caattcgatc tgcgaatagt cggcggacac gatgcggctg
     1081 cctgtcggtg cgatgaaggc ctcgcggatg cggcggccct cggcggtgcg caccgggatg
     1141 ttctggaggt tggggtcgct tgatgccagc cgcccggtga ccgcgaccgc ctgcgagtag
     1201 ctggtgtgca cgcggccggt agcgcggtcc accatctgcg gcagcttgtc ggtgtaggtg
     1261 gatttcagct tggccatgcc gcgatattcc agcaggattt tcggcagcgg atagtcgagc
     1321 gccagctcct gcagcacctc ttcgtcggtg gaagggtcgc cgctgggcgt cttcttcttc
     1381 accgggaggc ccagcttgtc gaacaggatc tcgcggagct gtttgggcga attgagattg
     1441 aacggttgtc ccgccgcttc gaacgcgcgg gcttcgagtg caatcagctt ctcgcccagc
     1501 tcgcggctct gcacctgcag cagcgcggaa tccagcagta cgccgttgcg ctccatgatg
     1561 tacagcacgg ccatggtcgg cagctcgatg tcgcggtaca catgctccag ccccgactgt
     1621 tccgcgatct gcggcgcgag ggtctggtgc agttgcagcg tgatgtcggc atcctcggcg
     1681 gcgtagcgcg tggcggtgtc gagatcaacc tggtcgaagc agatctgctt cgcgcccttg
     1741 cccgccactt cggcgtagct gacggtcttc acgttgagat ggcgcagcgc gaggttgtcc
     1801 atgtcgtggg gtttgtgcga ctccagcacg taggattgca gcagggtgtc gtcgtggatg
     1861 cccgccagat ggataccgtg attggcgaag atatgcaggt cgtacttgag gttctgcccc
     1921 agcttccggt gtttcgcgct ctccagccag ggcttgagct tttccagcgc gtgttcgcgc
     1981 ttgagctgat cgggtgcacc gggatagatg tgcgccagcg gcagataggc ggcctcgtgc
     2041 gccttgaccg aaaatgacat gcccaccagc tgcgcgttca tcacgtcgag gccggtggtc
     2101 tcggtgtcca cgcaggtcag ctcggcggcg agcagctttt ccagccaggc atccagctgg
     2161 gcgtcggtga ggatggtctc gtaatgggcg ctgtcagtgc gtgtgggttc gtcggcgaac
     2221 atgtcttgcg tggcgggagc cgttgcttcc gtcgtgctgc cggacgattt gggtgtgccg
     2281 cccagctcgc gcagccagct cctgaattcg aagcgctcgt acaggctgcg cagctgttcg
     2341 atgtctgctg gcggggcgat cattgcggtg tagggaaggg gcagattcac gtcgcacttc
     2401 accgtgatca gctggcgtgc ctgcggcagc cattccagcg cttcacgcaa gttggcgccc
     2461 actacgccgc tgacttcgtc ggcatgctgc atcaggttgt ccagcgtgcc gtactgggtg
     2521 agccacttca cggcggtctt cgggccgcac ttggtcacgc ccggcacgtt gtccacctga
     2581 tcgccggtca gcgtcaggaa gtcgatgatg cgctccggcg gcacaccgaa tttggcggtc
     2641 acgccgggga tgtccagcgt ctcgttgttc atggtgttga tcagcgtgac atgctcgttc
     2701 accagctggg cgagatcctt gtcgccggtg gcgacgatgc agcgcacgcc gtcacgggtg
     2761 gcgtgatctg ccagcgtgcc gatcacgtcg tcggcctcca cgcccggttg cgccagaata
     2821 tgccagccgg tggcgatgat ggcctgatgc agcggctcga tctgttgcgc cagatcgccc
     2881 ggcatggacg ggcgggtggc cttgtattcg ggatacatgt cgtcgcggaa ggttttcccc
     2941 ttggcatcga agacgcacag gctataatcc gccggatagt cctgccgcag tttgcgcagc
     3001 atgttgagca cgccgtagat cgcgccggtc ggaaaacctt cgcggttgcg catgtccggc
     3061 atcgcatgga acgcgcggta caggaacgat gaaccgtcga ccagaagtag tgttttggtg
     3121 aagttgtccg tattcatttc aataatttgc ttggggaaaa ttgcatgagc ataccgtcta
     3181 aattgcctgt cccggtcgtg ccggagaact ggaattccaa ggaggcatgg cgtgtgttcg
     3241 gcattatggc agagttcgtc tccgccaccg accgcctcaa ccgcattcat cccgcagtca
     3301 gcatcttcgg cagcgcacgc accaagccgg atcagcccta ttacaaactg gccgaagaga
     3361 tttcgcggct gttgtccgac gccggttttg cggtgatctc gggcgggggg cccggcatca
     3421 tggaggcggc caacaagggg gcgtattacg gcaagtcgcc cagcgtcggt ctgaatatcc
     3481 agttgccgca cgagcagaac accaatctct accagaacat cagccatacc ttccagcact
     3541 tctttacgcg caaggtgatg ttcgtcaaat tcgccagcgc ctatgtggtg atgcccggcg
     3601 gcttcggcac gctggacgag ctgatggaag cgctgacgct ggtgcagacc ggcaagaccc
     3661 gccgcatgcc gatcattctg gtgggcagca agttctgggc gggcatggtc gaatggttcc
     3721 gcacggcatt gctggaggag aaggtgatca gcccggagga catggatctg gtgcaggtca
     3781 tcgacgatcc ggaagcggtg gtcagcgcga tcttcaagca ttacgagacg cgcggcttcg
     3841 agccctcgga ggcggaacgc gagcgccagc tgtacctgta aatcggctaa aatacgcacc
     3901 taccaacctt cgaggctact gcgatgcgac tccctgccct tttgcttttg agcggcttat
     3961 cctttgccgc gttcgcccag ccgccggttc ccgcgaatct cgagccgctg ccaccgcctc
     4021 ccgcgttcga agagaaagac gcgccgggag agccggaggt gaccatcacc aaggaagctg
     4081 cccagaccgt ggaagaatac cgcgcgaacg gcaagctcta catgatcaag atcacgccca
     4141 aacatggcgt gccttactat ctggtggaca gcaagggcga cggcaaattc gcgcgtcagg
     4201 agagtgccga ttccggcttg cgcgtaccgc gctgggtcat ctttaaattc taaaatggcc
     4261 gtttttacca gcgtatccga ggcggagctg accgcctggt tgggtgatta ttcgctcggc
     4321 caactgctcg aattgcagcc tatcgcctcc ggcatcgaga acaccaacta cttcgtcacc
     4381 accggcaacg gtcgcttcgt gctcacgctg ttcgagaagc tgacggccga cgaactgccg
     4441 ttctatctga acctgatggc gcatctggcg cggcacggca tcccctgccc cagcccggtg
     4501 gcgaaccgcc tcaaccagtt tctgggcagc ctcaacggca agcccgcatg catcgctacc
     4561 cgcctctccg gcaagtccac caccgtaccg aacttggcgc aatgcgcggc catcggtgcg
     4621 atgctcggcc agttgcatct ggccggacag agcttcagcc agatcatgcc caaccccaga
     4681 ggggcggcat ggcgcgcggc gaccgcgccg caggtgcgcc ccttcctcga tgcggcccag
     4741 gccgagttgc tggatggcga agtggcgctg catgccggaa gcgatctgtc tcgcctgccg
     4801 cagggcgtga tccatgccga cctgttccgc gacaatgtgc tgctggaaga ggacagggtc
     4861 ggcgggctga tcgatttcta tttcgcctgc agcgacgcgc tgctgtacga cgtggcgatc
     4921 accctcaacg actggtgcat gccggaaggc aagctggatg ccgcccgtgc gcaagccttt
     4981 ctgcgcgcct atcatgcggt gcgtccgctg caggacagcg agcgcgatgc ctggccgttg
     5041 atgttgcggc tggccgcatt gcgcttctgg ctgtcgcgcc tgtacgacct gcatctgccg
     5101 cgcgacggcg agctgatcaa cgcgcatgat ccggggcact tcgaacgcat cctgcgcaac
     5161 tacatcgcga cggcacagcc cgtctggctc tgagtgtgaa ccatatggaa atacgcaaac
     5221 tgaacgccgc gcgcggctgg acttgggtga agcagggcta tcagctcatc atgcgcaacc
     5281 cgctgctgtc tgtttctctg gcgctgaccg gcgcgctggc gatattcgcc gtgctgcata
     5341 tcccgctgtt cggcccgctg ctcgcgattc tgttcatgcc ggtgctgatc gccggttaca
     5401 tgcgtgcctg ccgggcgctg gaagaaaacg aagaggtcga gctggctcat cttttcgccg
     5461 gattcgaaaa gcatgcggtg cccttgatct cgctgggcgg ctacatgatg ctggggctgt
     5521 tgatcgtctc gatggtgatg atcttcatcg gcggcgcggc cttcgccaaa ctgctggaaa
     5581 gctttcaggc cagcaacgac ccgcagctgc tgatcgcggc gttgggcagc gccggctcgg
     5641 gcgtggcgtt cacgctgctg gtcggtttct cgctggtgtt cgtgttgatg ctggcattgc
     5701 agtacgcgcc gatgctggtg ttcttcagcg acgtgaagcc gctcgccgca ttgcgtgcca
     5761 gcctcgccgg ttcgctgcgc aacatcatcg cgtattcggt ctacagcctc atcatgcagg
     5821 tggtcgcact ggtgctgggc gtgctgccct acggcatcgg gatgatcgcc ttgctgccgc
     5881 tgggcttcac ctcgctctac gtcagctacc gcaacatctt ccccttcccc ggtgagatac
     5941 cgcccgccgc agagtgagcg gtctttccgc aagccggtct cgcccgccaa acattagggt
     6001 gtgctaatat tttcgccgtg gccggcaact gttcggcagg cagcggagac ggcaatgcaa
     6061 tttcaggcaa gtatcgagga agtaaagcgc gcacaggctc cacacaacct gttcatatcc
     6121 ggtctgttcc tgttcaatct gctgatgacc ccggcgatca ttgccttgaa agtcggcctg
     6181 atcggcgtgc tgattccgct gttctgttcc ggcgcgctgc tgggctacat ctatctgcgc
     6241 agccgcagaa ccaccagctg gttcgtcgac gcccattggc gtctgtcgtt tcgccacgga
     6301 aaatggctga tgctgggcta cgcgatctcc gcatcgctga ttctgctggc ctggctgctc
     6361 agcctgacgg cccatgaggc gagcatgaaa cacatcatgc tgaccgctct cacccgcatc
     6421 gccgtgatac cgaccctgat cgcggtgatg gtcacgctcg tgctggaatt cggcgctgcg
     6481 gcccaggccg tcaagagcga agtgccggat ggcatggtcg cgaaattccc gccgccagcc
     6541 gcctagagcg gctgccagtc ggagaaggtt gccgcctggc cgtggagctt gcccgcctcg
     6601 tcgagcagat tccacaccgt cgccgcgttg atcgtgaagc gtttgccgct cgcgccgatg
     6661 cgcactcccg cgtagtcgtc cacatagcct cgttgcgtca cgcgctccag cagtcttgcg
     6721 cgctcggccc gctccagcgg ctcggcggag tagcgcgacg gcaaccgggt gaacgccgcg
     6781 aaatccatgt cgaacaattc cagcgccaga cggttgccgt agaaaaacac cgggtcgtcc
     6841 tcggtgccgt gcgcgacgat ggcgcggggg gcttcccaca gcgcttgttt agtgggagcg
     6901 gcttcagccg cgattcctcc atcatgcaaa gatgccatcg cggctgaagc cgctcccacg
     6961 aaggttgcag cctgtggatc gagtagttcc ttgccggtta gcctgcgata gctgtcgacg
     7021 atcagccgca ggcgttcgtc cagcgcaggg tcagacggcg gtgagcttcg catattccag
     7081 cgacagccac ttgctgccgg catgcttgaa attgacctgc acgcgcccgt cggtgcccgt
     7141 gccctcgacg ccggtgatga cgccctcgcc gaacttctgg tggaacacgc gctggccgat
     7201 gcgccatgcg ctctcgtcat tggcgcgggc aggtgctgcg ccgccgccga aggagggcgc
     7261 ggaggaggaa ggggagtcga ggtagctgtc gaagcgcttg gccgtgacga accggggcga
     7321 cagccagcgc agcaattctt ccggcagctc gttcaggaag ctcgacgccg tgctgtaact
     7381 cgccttgccg tgcagcatgc ggcgctgggc gaaggtgagg tagagctggc ggcgtgcgcg
     7441 ggtgatcgcc acgtacataa gacggcgctc ctcatccagt ccgccgtcca cgttctggct
     7501 gttctggtgc gggaacaggc cgtcttcgag gccggtgatg aacacggtgt ggaactccag
     7561 ccccttggcg gcatgcaccg tcatcagatg cagcgcgtcg tcctgatcgc ccgcctgatg
     7621 ttcgcccgcc tccagcgagg cgtgggtcag gaaggaggtc aggctgtcgt cctcgttctc
     7681 gtgtacgaac atcgtcgccg agttgatcag ctcgttcagg ttctccagcc gctcctcggc
     7741 ctcgcgcttc ttcgcgccgg tctcgttctt gaaatgatcg accagcccgc tgtgcgtcag
     7801 cacatggtcg atgatctcgg gcagcggcaa atccttcgtc gcgctgcgca gcgattcgat
     7861 cagtgcgacg aaggccgaca ccttgccgcc cgcgcgcgcc gctgcatccc acagggccgt
     7921 attgttttgt ctggccgcgt cctgcaactg ttcgatgctg cgcgcgccga tgccgcgcgt
     7981 ggggaaattg atgatgcgca acagcgcgtt gtcgtcgtcg gtgttctcca tcaggcgcag
     8041 gtaggccagc gcgtgcttga tctcctgccg ctcgaagaag cgcaggccgc cgtacacgcg
     8101 gtacgacagt cccgccgata ccagcgcgtg ttccagtacg cgcgactgcg cgttggagcg
     8161 atacagcagc gcgatctcct tgaggtggat gccctcgcgc tggagctgct tgatctcgtc
     8221 gacgatgaag gccgcctcat ccacatcggt cgccgcctcg taggcgcgca gctgctcgcc
     8281 gccgctctcg gcggtccaca gattcttgcc cagacggttg cggttgttgc agatcagcgc
     8341 gttggccgcg tcgaggatgt tgccgtgcga gcggtagttc tgttccagct tgatcacgtt
     8401 cgcgacatgg aagtcgcgca gcaggtcctg catgttgccc acgttggcgc cgcgaaacgc
     8461 gtagatcgac tggtcgtcgt cgcccaccgc gaacagcgcg ttgctgcgcc cggccagcag
     8521 cttcagccag ttgtattgca gcggattggt gtcctgaaac tcgtccacca gaatgtgctt
     8581 gaagcgatcc tgatagtgct cgcgcagctc ggcattgcgc gacagcagct cgtagcagcg
     8641 cagcaagagt tcggagaaat ccaccacgcc ctcgcgctgg cactgcgcat cgtattcggc
     8701 ataaacctcg accttgcgcc gggtgtaggg atcgtaagcc tccacctcat gggcgcgcag
     8761 cccctgttcc ttgctgccgc tgatgaagtt ctgcatctcg cgcggcggat atttctcgtc
     8821 gtccacgttc aactgcttca tcacccgctt gatcgcggag agctggtcgc cggaatcgag
     8881 gatctggaag gtctgcggca ggttcgcctc gcggtggtgg gcgcgcagca tgcggttgca
     8941 cagtccgtgg aaggtgccga tccacatgcc gcgcgtgttg atcggcagca tcgcggacaa
     9001 gcgggtgacc atctccttag ccgccttgtt ggtgaatgtc accgccagca gcccgtgcgg
     9061 gctgacctgc ccggtgctga tcagccaggc gatgcgcgtg gtcaggacgc gcgtcttgcc
     9121 gctgcctgcc cccgccagga tcagggcgga ttgggcggga agttcgacgg cggcacgttg
     9181 ttcgggattg agaccggaaa gcagggaaga attcatgggc gaattatccc acaggaggcg
     9241 atatggcact caaggcatga cgcgaacgct acgactggat tacttgcggc gcatgctcat
     9301 atgcagggtg gtggcctcgc cgacttcgat gcgctcgtat tcggcgatca cctgtgcgcc
     9361 cagcaacagc agggtggccg caacttccag actgatcagc aggacgatgg tggtggtgag
     9421 tgagccgtat acctcgccca cctgcgacaa cgagccgaaa taccagcgca atagaaagcg
     9481 gacgacttcc cacagcaagg cggccgtgac gccgccgatc agcgcgtgat ggaccggcag
     9541 cctgcccacc ggcatgaaga aatagagcgc ggagacgagc aggacctcgc cgatgagacc
     9601 gccgccgtag atcagcgcat gcgagaaggt gtccagtgac cagctctgcc cgaacagagc
     9661 gacgttcccg ctgcccagcg ccaccagatc ggcgatcagg attgccccgg tgaacagcag
     9721 cgtgcagata aaagtgatgt agccgaaggg caatagcagg gaaacgaaga aattgcgctt
     9781 gagcttctgc atccggtggc gaaagatcac cgacatcgcg ctttcgagca cccggaatcc
     9841 cagcgaactg aagaacagca gcgtgataag cagcagccat ccgatcactc cgccgtgttc
     9901 gaggaacgct ttcagttccg tcatgaccgt ccggttctcg cccggcgcca cgtatctcag
     9961 tgcccagcgc accgtctcca gcagcgcggc ctgatcgatg aactgcgaca gggcgatcac
    10021 catgaggatg accatgggca cgatggagag cagcgcgtag taggccaccg caccggcgag
    10081 cagcaggccc tgattctccc ggaacgcctt cagcacctgc agcgtgaagg agagcgggtg
    10141 tctcaggaca tgcactgtgc gggggctgac aagttgcatg ggtggctgga agcctcctgt
    10201 attaattcgg tcggtgccgc gatagtaagg catggcgcag catgcaacaa acattcatgg
    10261 gcagatacag gccatcccga tattctgcga caggcatctg ctgtcattct ccccggcatg
    10321 gtgacccgcg cgctagaatg ctcgcccgcc agtcgccgaa cttacagagc cgccccatga
    10381 acgatgaacg cttgatcgat atcgaaacca agatcgccta ccaggaagac ctgatcgagg
    10441 aactgaacaa gacgatctac cagcagcaga agaggctgga gcgtctggag gcggtttgcc
    10501 aaacgcttgc cggacacatc gaatccctga atgcggcaaa gaacgatggc atgcccgcga
    10561 acgaaagacc gccgcactac tagcccgccg ccatgctgaa cagactgaat gacttcctgg
    10621 ctacgatcat cacccccgcc gagggtcgtc ccgaacacac cctgcaactg gcgaccgccg
    10681 tgctgctagt cgaagtgatg cgttcggacg ccgaaagcac ggatgaagag caggccacga
    10741 tactggacat cctcaagcag cggttccagc tcgcggacgc cgaagtggcg cagctgaccg
    10801 agctggcgca ccgcacggca aagtccgcga ccgatctcca ccagttcacc tcgctcatca
    10861 accgcgagct ggcagcgccc gaaaagctgc gcatcatcga atacatgtgg caggtcgcct
    10921 atgccgaccg gcagatcagc gcccacgaga accacctgat gcgccggatg gccgaactgc
    10981 tgcacatcgg ccacggcgac tacatcgccg ctaagatgcg tgcgaaacct gcggagctga
    11041 actaaccacg tagtcagcat gatgggcgcg cgttaccttc gcaggaatca tagggacgat
    11101 taatttgccc aatctgcgga aagtcgatcc gcctgttcca gcaccagttg tatagccgct
    11161 tcttgcaggt ctggcggata tttgaagcgg cgcaaggtga tgcgcaccaa gttgcgcaaa
    11221 cgagcacgca cactgtcgcg cttctgccag tccactgtcg tgctgttgcg cagtttgtcg
    11281 gtcagttcgt gtgcaatctg tttcagaatc tcatcgccaa gttcgcgcac cgcgctttcg
    11341 ttatccgcca gtgcatcata gaaggcgagc tcagactcgt ttaggccaag ctcatctcca
    11401 cgcttggcgg cctcacgaaa ctcccttgcc atcgcaatta attcttcgat gacttgtgcg
    11461 ctctcgatgg cacggctgcg gtagcggttc agcgcagcca gcaggcgctc gctgaatttc
    11521 ttctccagca acacattggt atgcatgcgc gacctgatct cgtcgttcag cagcttttgc
    11581 agcaactcca cggcaaagtt gcgctgcggc agttggcgta cttcttccag gaaaccatcc
    11641 gaaagaatac cgatgtcggg tcgctccagg ccggccagct tgaagatgtc ctccacgcct
    11701 tccgcgacca ccgcattgtc gagtatctgt ttgagcaccg cattcttgtg ctcttccgtc
    11761 agcttcttat ctgtttcggt agctttggca atgaccgctt tgatcgcctg gaagaatgcg
    11821 atttcctcgc gcattgccat cgcatcgccc agcgtgccgc atagcgagaa tgccttggtg
    11881 attttcagca ccacgtcgaa ccagcgtttc ttgccgtctt ccagacccag cacgaagttg
    11941 gctgcaggca gcagcaatgt ggtcgcctga gtacgataaa ccgagtaatc gaactcgctc
    12001 agaatgtcgc gcgccacctc cagatattcc tgtaacttcg ccagtgcctc ggaagcgtcc
    12061 accgtcggca tacccttgcc ccagctctcg gtatagacct tcaatgcctt
//
LOCUS       DASUIV010000281         6273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1191188, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000281 DASUIV010000000
VERSION     DASUIV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191188"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFW59_10125"
     CDS             complement(<1..181)
                     /locus_tag="VFW59_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364612.1"
                     /translation="MVDREARNKLAEAMRALASGLITNDEFEDKRLPHSKEDPAISEV
                     FSKGAWLLYSDLEEYR"
     gene            complement(396..3101)
                     /gene="glnE"
                     /locus_tag="VFW59_10130"
     CDS             complement(396..3101)
                     /gene="glnE"
                     /locus_tag="VFW59_10130"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX5364613.1"
                     /translation="MNTALPAFDILLQKTRSCSRYARRMLDMDPQLTDWLAANYASVC
                     DRSEMLGLLHGFNLDDEAELSRAVRRLRKQVMVKLILRDLNGLCDLNEVMQAMTALAE
                     VCVQQAQSCLMRALQEQYGRPLGETDGTPQELLVIGMGKLGGGELNVSSDIDLIFVYP
                     EDGETDGARSLSNHEFFTKLGRRLINLINDLTADGYVFRVDMRLRPYGDSGPLVMSFA
                     ALEEYLVSQGREWERYAWIKARVISPAEHPEIAELSKLAQPFVFRKYLDFGAIDSMRK
                     LHAQIRQEVQRRERLNNIKLGPGGIREIEFTAQVFQLIRGGRDAKLRIRPTQQVLKLL
                     VANQQLDAQTAADLDAAYIFLRNLEHRLQYLDDQQTQELPDKPEDQTVIAEAMGYADY
                     AALLVPLDRHRALVSQQFERIFGEQEGESGDEQHGTFPWREGVTAGELTAYMENLGYR
                     AAPDAGQRLLQLREGGRYRQLPDLSRQRLDRLIPQFIAQSAQQSDPDATLMRALTLLE
                     GISRRASYLAFLAEYPQALQRLVRLIAASSWAADYMAQHPALFDELLDAREIYQPPQW
                     QQIDRDLQQRLDDCDGDVERQLDVLRQTQQEQTFQLLVMDLQGLLPLEKLSDHQSDLA
                     DLLLRHVLALSWATSRKRHCEQPKFAIIAYGKLGGKELGYASDLDLIFLYDDDHPDAQ
                     ENYARLAQRINTVLSSHTASGRLYETDLRLRPNGASGLLVSSVEAFADYQQKQAWVWE
                     HQALTRARFSAGDAGIGAAFEKIRREVLCQPRDLAKLREEILSMRQKMHDGHPNDTAL
                     FDIKHDKGGMVDIEFMVQYLVLAHAHEHPELTANIGNLALLKLSGELKLIPADLAKQV
                     SELYRTLRQTQHRMRLNSSAPCRVAQEEIDTGACRELWKLVFEGN"
     gene            3179..>6273
                     /locus_tag="VFW59_10135"
     CDS             3179..>6273
                     /locus_tag="VFW59_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEX5364614.1"
                     /translation="MLNSLPVRLLWHSLNWLTRFAIVASGIFAVLMAIAILVLRYWLM
                     PDVGQYHDKIVASLSAAIGNPVTIARIEGDWRGFRPRLNFSGVRILDDRHQVALELPN
                     VDSSVSWLSLFTGELRLASLEIERPELLIRRDAQGKFFIGGVALTAQGSDNDLSDWLL
                     RQSRMVARDALIVWLDEQRDAPPLVLNHVNLRIESFLGNHRFALQAQPPEALSTPLDV
                     RGDFHGKSFDDLSEWRGQIYTRLDHTDVTAWRPWLDLPEEFSRGRGALRGWLGIERGR
                     VSEITADVNLRDVATRLAEDLPELLLHNVHGRAQWKASEGDLEVSTKRLSMRLHNGIV
                     LPPTDFYLRLTGGRGEPAGGEMQANLLQLEILSSLAAFVPLDASLRAQLEAYAPKGRV
                     ANLNVSWQGASAKPLSYRIKGKFDNLALRQVGSLPGFSGLSVDVDGSQTGGRLNIDSR
                     QLVVDAPGAMVEPLSFATLTGQASWQQEGSELLLRADNVAVSNADLAGNLYGSYQTKQ
                     GTLGLLDLTIALTRGDVTKAYRYTPLVALDKEDNDWLKEALLTGHTEDFRVRVKGNLS
                     DFPLDGKKDGLLEIGGHAQGVELAFHKDWPHVDNIAGEFWIRGNKLEVKAPAATMLGA
                     QLQNLSVALPDLMSVDLPLEVRGVALASNDIFLNFIQHSPVRGYIDGFTDDLKASGSS
                     HLGLFLRVPLLGEKPVKVEGSLRVDGSDIDLGEGAPLLRNTRGTLSFTESGIKADNVS
                     SEVLGGKARIDLKSEEGGVLHAKAQGRCDLDALRRSNPQPLLNRLRGGAAWDADITVV
                     KKFTEMLITSNLQGIASSLPAPFAKRATEQMPLRLEKKIVTEGQDAIEFQLGKLLGAR
                     LARREENGAMAIKRGVVSLGTQTDTGKWMKRDGVWLVGQLPQLSLEGWGDLLGAAGGA
                     SDLPIAGADFRVAKVSGFGAQINDLGVEASRRGEGLVANLSSSTLNGDLEWQPRGDGK
                     LTVHLQNVEWGGANHSPAVVAKAAPTPASTLSPAQLPAMQIAVDNLKVDGKQVGRVEL
                     VG"
BASE COUNT         1092 a         1827 c         2097 g         1257 t
ORIGIN      
        1 agcgatattc ttcgagatcg gaatatagca accaagctcc cttggaaaac acctccgaaa
       61 ttgcggggtc ttctttgctg tgaggtagtc gtttgtcctc gaactcgtca ttagtgatga
      121 ggccagaggc taaagcccgc attgcctccg ctaatttgtt tcgtgcttct cggtcaacca
      181 cggcaatctc ctgatgcact ctaactaaaa atagaaaaca gtttcatagc gcggcaatgt
      241 cgcctatatc cagcacctct gtcaaagatt tattgatatt catgccatta gcataagttg
      301 ctcgatggat ttccgtatta ggcagcctaa aatttcgcgg atggaacctc gggtgaggct
      361 ttaactcacc tcatcccgct atcccggcaa agtgcttaat ttccctcgaa caccaacttc
      421 cacaattccc tgcacgcccc cgtgtcgatc tcttcctgcg cgacccggca gggcgccgag
      481 ctgttcaggc gcatgcggtg ctgggtctgg cgcagggttc ggtagagttc gctgacctgt
      541 ttcgcgaggt cggcggggat caatttcagt tcgcctgaaa gtttcagcag cgccaggttg
      601 ccgatgttcg cggtcagttc gggatgttcg tgggcatgcg cgagcacgag gtattgcacc
      661 atgaattcga tgtccaccat gccgcctttg tcgtgcttga tgtcgaacag ggcggtgtcg
      721 ttcggatggc cgtcgtgcat cttctggcgc atcgagagga tttcttcgcg cagcttggcg
      781 aggtcgcgcg gctggcacag cacttcgcgg cgtatctttt cgaaggctgc gccgatgccg
      841 gcgtcgcccg cgctgaagcg cgcacgggtc agcgcctgat gttcccagac ccaggcctgt
      901 ttctgctggt agtcggcgaa ggcctcgacc gagctgacca gcaggccgct cgcaccgttc
      961 gggcgcaggc gcaggtcggt ctcgtacagg cggccggagg cggtgtggct gctcagcacg
     1021 gtgttgatgc gctgcgcgag ccgcgcatag ttctcctgcg cgtccgggtg gtcgtcgtcg
     1081 tagagaaaga tcaggtcgag gtcggaggcg tagcccagct ctttgcctcc cagtttgccg
     1141 taggcgatga tggcgaactt gggctgctcg cagtgacgtt tgcgcgaggt ggcccagctc
     1201 aatgccagca catggcgcag cagcaggtcg gccagatcgc tctggtgatc gctgagtttt
     1261 tccagcggca gcaggccctg cagatccatc accagcagct ggaaggtctg ctcctgttgc
     1321 gtctgacgca gcacatccag ctggcgttcg acgtcgccat cgcaatcgtc cagacgctgt
     1381 tgcagatcgc ggtcgatctg ctgccattgc ggcggctggt agatttcgcg ggcatccagc
     1441 agctcgtcga acagcgcggg gtgttgcgcc atatagtcgg cagcccagct gctggcggcg
     1501 atcagccgca ccagacgttg cagcgcctgc gggtattcgg ccagaaaggc gaggtaggag
     1561 gcgcggcggc tgatgccttc cagcagcgtc agcgcgcgca tcagcgtcgc atcggggtcg
     1621 ctctgctgcg cgctctgcgc gatgaattgc gggatcagcc ggtccagtcg ctggcggctc
     1681 aggtcgggca gctggcggta acgtccgccc tcgcgcagtt gcagcaggcg ttgtccggca
     1741 tccggcgcgg cgcggtaacc gaggttttcc atataggcgg tcagctctcc ggcggtcacg
     1801 ccttcgcgcc aggggaaggt gccgtgctgt tcgtcgcccg attcgccttc ctgctcgccg
     1861 aagatgcgtt cgaactgctg gctgaccagc gcgcggtggc ggtccagcgg caccagcagg
     1921 gcggcatagt ccgcatagcc catcgcctcg gcgatgaccg tctgatcctc gggcttgtcg
     1981 ggcagttcct gcgtctgctg gtcgtccaga tattgcagcc ggtgctccag attgcgcagg
     2041 aagatatagg cggcatcgag gtcggcggcg gtctgggcgt cgagctgctg gttggcgacc
     2101 agcagcttca gcacctgctg ggtggggcgg atgcgcagct tcgcatcgcg tccgccgcgt
     2161 atcagctgga acacctgcgc ggtgaattcg atctcgcgga tgccgccggg gccgagcttg
     2221 atgttgttga ggcgttcgcg gcgctgcact tcctgacgga tctgcgcgtg caatttgcgc
     2281 atcgaatcga tcgcgccgaa gtcgagatat ttgcggaaca cgaagggctg cgcgagtttt
     2341 gacagctcgg caatttcggg atgctcggcc ggcgagatca cgcgggcctt gatccaggca
     2401 tagcgctccc attcgcggcc ctggctgacc agatattctt ccagtgcggc gaagctcatc
     2461 accagcgggc cgctgtcgcc gtagggacgc agccgcatgt ccacgcggaa cacgtaaccg
     2521 tccgcagtga gatcgttgat gaggttgatc aggcggcgcc ccagcttggt gaagaactcg
     2581 tggttggaaa ggctgcgcgc cccgtcggtc tcgccgtctt ccgggtagac gaagatcagg
     2641 tcgatgtccg aggagacgtt cagttcgccg ccgcccagct tgcccatgcc gatcaccagc
     2701 agttcctgcg gcgtgccgtc ggtctcgccc agcggtctgc cgtattgttc ctgcaaggcg
     2761 cgcatcaggc agctttgcgc ctgctgcacg catacttcgg ccagcgcggt catcgcctgc
     2821 atcacctcgt tcaaatcgca caatccgttg aggtcgcgca ggatcagttt gaccatcacc
     2881 tgcttgcgca gcctgcgcac ggcgcgcgac agttcggctt cgtcatcgag attaaaaccg
     2941 tgcagcaagc ccagcatctc gctgcggtcg cagacgctgg cgtagttggc cgcgagccag
     3001 tctgtcagct gcggatccat gtccagcatc cgccgggcgt agcggctgca cgatctggtt
     3061 ttttgcagca ggatgtcgaa ggcgggaagt gcagtattca tggagtgatt cacgctagaa
     3121 tgtcggtcgt ctcagattat aagaccagac caccgcgcgt tgcgccttta ctcatccgat
     3181 gcttaattcg cttcctgtcc gcctgctgtg gcacagcctg aactggctga cccgtttcgc
     3241 catcgttgcg tcggggatat tcgccgtgct gatggcgatc gccatcctcg tgctgcgcta
     3301 ttggctgatg cccgatgtcg ggcagtatca cgacaagatc gtcgcctcgc tgtctgccgc
     3361 gataggcaat ccggtcacca tcgccaggat agaaggcgat tggcgcggat tccggccgcg
     3421 tctgaatttt tccggggtgc gcatcctcga cgatcggcat caggtcgcgc tggaattgcc
     3481 gaatgtggat agcagcgtgt cctggctgtc gctgttcacc ggcgagttgc ggctggccag
     3541 tctggagatc gaaaggccgg agctgctgat ccgtcgcgat gctcagggca agttttttat
     3601 cggcggcgta gcgctgaccg cgcaggggag cgacaacgat ctgtccgact ggttattgcg
     3661 ccagtcgcgc atggtggcgc gcgatgcgct catcgtatgg ctggatgagc agcgcgatgc
     3721 ccctccgctg gtattgaacc acgtcaatct gcgcatcgag agttttctcg gcaaccatcg
     3781 cttcgcgttg caggctcagc cacccgaggc gttgtccacg ccgctggatg tgcgcggcga
     3841 ttttcacggg aagagcttcg acgatctgag cgagtggcgc ggccagattt acacgagact
     3901 ggatcatacc gatgtgacgg cctggcgccc ctggctggat ctgcccgaag aattcagtcg
     3961 cgggcgcggc gcattgcgcg gctggctggg catcgagcgt ggccgggtga gcgagatcac
     4021 ggcggatgtg aatctgcgcg acgtggcgac ccggttggcc gaagatctgc cggagttgtt
     4081 gctgcacaat gtgcacggac gcgcacaatg gaaggccagc gagggcgatc tggaagtttc
     4141 caccaaacgc ttgtcgatgc gtttgcacaa cggcatcgtg ttgccgccca ccgattttta
     4201 tctgcgcctc accggaggcc ggggcgagcc ggctggcgga gagatgcagg ccaatctgtt
     4261 gcagctggaa atactgtcca gcctggcggc tttcgtgccg ctggacgcca gcctgcgcgc
     4321 ccagctggaa gcctatgcgc ccaagggcag ggtggctaac ctgaatgtca gctggcaggg
     4381 cgcttcggca aaaccgctgt cataccgcat caaggggaag ttcgacaatc tcgcgctgcg
     4441 tcaggtcggc tccctgccgg gtttctccgg gctgtcggtg gacgtggatg gcagccagac
     4501 tggcggccgg ttgaacatcg actcgcgcca gctggtggtg gacgcaccgg gtgcgatggt
     4561 ggagcctttg tctttcgcca ccctgaccgg gcaggccagc tggcagcagg aaggcagcga
     4621 gctgctgctg cgggccgata acgtcgcggt gagcaacgca gacctggcgg gcaatctgta
     4681 tggcagttac cagaccaagc agggcacgct gggcctgctc gatctgacca tcgcgctgac
     4741 gcgcggcgat gtcaccaagg cctatcgcta tacgccgctg gtcgcgctcg acaaggaaga
     4801 taacgattgg ctgaaggagg ctctgctgac ggggcatacc gaggacttcc gcgtgcgggt
     4861 gaagggcaat ctcagcgact ttccgctgga cggaaaaaag gacgggctgc tggagatcgg
     4921 cggtcatgcg cagggcgtgg agctggcatt ccacaaggat tggccgcatg tcgacaatat
     4981 cgcgggcgag ttctggatac gcggcaacaa gctggaggtg aaagcgcctg cggcaaccat
     5041 gctgggggcg cagttgcaga atctgagcgt tgcgttgccg gacctgatga gtgtagacct
     5101 gccgctggaa gtcaggggcg tggcgctggc atcgaacgac atcttcctga acttcatcca
     5161 gcacagcccg gtgcgcggtt atatcgacgg ctttaccgat gacctcaagg ccagcggaag
     5221 cagtcacttg ggcttgttcc tgcgggttcc cttgctgggt gagaagccgg tgaaggtgga
     5281 gggctcgctg cgcgtcgacg ggagcgatat cgatctgggc gaaggggcgc cgttgctgcg
     5341 caacacgcgc ggcaccctgt cgtttaccga atcgggaatc aaggccgata acgtgtcgtc
     5401 ggaggtgctc ggcggcaagg cacgcatcga cttgaagagc gaagagggcg gggtcttgca
     5461 tgccaaggcg caagggcgct gcgatctcga tgcgctgcgc aggagcaatc cgcagccgct
     5521 gttgaaccgt ttgcgtggcg gggcggcatg ggatgccgac atcacggtcg tgaagaaatt
     5581 cacagaaatg ctcatcacct ccaatctgca ggggatcgcc tccagcctgc ctgctccctt
     5641 cgccaagcgc gcgaccgagc agatgccctt gcgtctggag aagaagatcg tgacggaagg
     5701 gcaggatgcc atcgagttcc agctgggcaa gctgctcggc gcgagactgg cgcggcgcga
     5761 agaaaacggc gcgatggcga tcaagcgcgg ggtggtcagt ctggggaccc agaccgatac
     5821 cggcaagtgg atgaagcgcg acggcgtctg gctggtcggc cagctgccgc agttgtcgct
     5881 ggagggatgg ggcgatctgc tgggggcggc gggcggtgcc tcggatctgc ctatcgcggg
     5941 cgccgatttc agggtcgcga aggtgagcgg tttcggcgcc cagatcaatg atctgggcgt
     6001 ggaggccagc cggcgcggcg aaggcctggt ggcgaacctg tccagcagca cgctgaacgg
     6061 ggatcttgaa tggcagccgc gcggcgacgg caaactgacg gtgcatttgc agaatgtcga
     6121 atgggggggc gccaatcatt cgcccgccgt ggtggccaaa gccgcgccca cgcccgcgtc
     6181 cacgctttcg ccagcgcagc tgcctgcgat gcagatcgcg gtggacaacc tgaaggtcga
     6241 cgggaaacag gtcggccggg ttgaactggt cgg
//
LOCUS       DASUIV010000282         6074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1002972, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000282 DASUIV010000000
VERSION     DASUIV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002972"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /gene="infB"
                     /locus_tag="VFW59_10140"
     CDS             <1..1530
                     /gene="infB"
                     /locus_tag="VFW59_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5364615.1"
                     /translation="AADEAIVPRPVVVTVMGHVDHGKTSLLDYIRRTRVVSGEAGGIT
                     QHIGAYHVDTPRGMVTFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAI
                     AHAKAGNVPIVVAVTKIDKESANSERVKQELVAEGVVPEDWGGDTMFVEVSSKSGQGI
                     DKLLESVLLQAEVLELKAPRTTHAKGLVIEARLDKGKGPVATVLVQSGTLRRGDSVLV
                     GSVFGRVRAMLDEAGKTITEAGPSIPVEIQGLSEVPGAGEEMMVMADERRAREIALFR
                     QGKYRGVKLAKQQAAKLENIFEQMGEGEVSTLSLIVKSDVQGSAEAIAHSLLKLSTNE
                     VKVNLIHNAVGAISESDINLALASKAIVIGFNTRADAAARKLAESAGVDIRYYTIIYA
                     MIDEIKAAMSGMLAPEKRESVIGMVEIRQVFTVSKIGSIAGCYVLEGIVKRNAKVRVL
                     RNNVVIHDGELDSLKRFKDDAKEVKAGFECGLSLKGYNDIEVGDKLEVYEIIEVARAL
                     L"
     gene            1530..1895
                     /gene="rbfA"
                     /locus_tag="VFW59_10145"
     CDS             1530..1895
                     /gene="rbfA"
                     /locus_tag="VFW59_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5364616.1"
                     /translation="MANFARTDRIGQQIQRELAELVRLEISDPRVRLVTITGVEVAND
                     YSHAKIFFTRLDGKQEEAQQGLERASGFLRSKLARSLKLRIMPQLHFVFDASVERGSH
                     LSQLIDQAVASDQQLADKK"
     gene            1901..2842
                     /gene="truB"
                     /locus_tag="VFW59_10150"
     CDS             1901..2842
                     /gene="truB"
                     /locus_tag="VFW59_10150"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5364617.1"
                     /translation="MARTFRPLDGVLLFDKPLELSSNTALQKVRRLFQAEKAGHTGTL
                     DPLATGLLPICFGEATKFSTALLDADKTYRATLRLGQTTTTGDAEGEIVSESPVVVVQ
                     ADIDAALEKFRGEIRQLPPMHSALKHLGKPLYEYIRKGETVERELRNVVIHELRMERF
                     EGNQLDITVRCSKGTYVRTLAEDIGAALGCGAHLIALRRTAIAHFDLCDACDLQQLEA
                     MSQEQRDARVLPLESLMPDMPRLQLDAVQIKRMAQGQRLGMDTALPNGKVGLYGSQGF
                     IGVGMLDGRRLAPERLLSSVAKQAARPDLEQNISVEQ"
     gene            2891..3160
                     /gene="rpsO"
                     /locus_tag="VFW59_10155"
     CDS             2891..3160
                     /gene="rpsO"
                     /locus_tag="VFW59_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5364618.1"
                     /translation="MAITAAQKAQIVSDFQRAKSDTGSPEVQVALITARIVYLTEHFK
                     DNAKDHHSRRGLLRLVSRRRKLLDYLKSQNDAAYRSLIERLNIRK"
     gene            3260..5383
                     /gene="pnp"
                     /locus_tag="VFW59_10160"
     CDS             3260..5383
                     /gene="pnp"
                     /locus_tag="VFW59_10160"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5364619.1"
                     /translation="MSHYKKTIAYGNHQLTLETGEIARQASGAVMVSLDDTVVLVTVV
                     GRKDAKPEQDFFPLTVDYQERTYAAGKIPGGFFKREGRPSEKEILTSRLIDRPLRPLF
                     PESFYNEVQIVATVMSSDAEIDSDIPAMIGASAALALSGIPFDGPIGAARVGYADGKY
                     LLNPTATELKTSLMDLVVAGTDRAVQMVESEAQQLSEEVMLGAVVFGHEQMQVVIQAI
                     IEMADAVGAPAWDWVAPAKDEALIAQITAIAEAELQQAYAIRSKQARTDAVAAIQSKV
                     MAAVVTPESAPGQKNHVNDLFSALEAKIVRGQILSGEPRIDGRDTRTVRPITIRNGVL
                     PRVHGSSLFTRGETQAIVVATLGSMRDSQKIDALQGEYSDRFMLHYNFPPYSTGETGR
                     VGTPKRREIGHGRLAKRALVAVLPSEEDFGYAMRVVSEITESNGSSSMASVCGGCLSL
                     LDAGVPLKAHVAGIAMGLIKEGNRFAVLTDILGDEDHLGDMDFKVAGTEAGVTALQMD
                     IKINGITKEIMQAALVQAREGRMHILGLMKQAMPEARTEVSDFAPRMIKMKINPEKIR
                     DVIGKGGAVIRTLTEETGTTIDIDDAGNITISCVSGEAGELAKKRIEDIVAEVEVGKI
                     YEGPVVKLLDFGALINIMPGKDGLLHISQIAHERVNAVTDHLKEGQIVKVKVLEVDDK
                     GRMKLSMKVLLPAPEAAPAATPAAE"
     gene            complement(5521..5898)
                     /locus_tag="VFW59_10165"
     CDS             complement(5521..5898)
                     /locus_tag="VFW59_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364620.1"
                     /translation="MASQASSLCCSGQIIRCSEFSKDGWSELDSRKADERSCLAVLKR
                     RYRQLCLPIVRYWEANLRIQLSQTGYQQAFRFTIFPWFDGQLSALQQSFRFNTLMVGS
                     GSIVRSRISRKLPFEVLLCANGW"
BASE COUNT         1313 a         1681 c         1880 g         1200 t
ORIGIN      
        1 gctgcagacg aggcaatcgt tccgcggccg gtcgtggtga ccgtgatggg ccacgtcgat
       61 cacggcaaga cctcgctgct ggattacata cgccgtaccc gtgtggtttc cggtgaggca
      121 ggcggtatca cgcagcatat cggtgcgtat cacgtcgata caccgcgcgg catggtgacg
      181 ttcctcgata ccccgggtca cgaggcgttc accgccatgc gtgcccgtgg cgccaaggcg
      241 accgacatcg tgattctggt ggtggctgcc gacgatggcg tgatgccgca gaccaaggaa
      301 gcgatcgccc atgccaaggc cggcaatgtg ccgatcgtgg ttgcggtcac caagatcgac
      361 aaggaatccg cgaacagcga acgcgtcaag caggaactgg ttgccgaggg tgtcgtgccg
      421 gaagattggg gcggcgacac gatgtttgtc gaagtctcct ccaagtcggg gcagggcatt
      481 gataagctgc tggagagcgt gctgttgcag gccgaagtgc tggaattgaa ggcgccgcgt
      541 acgacccatg ccaagggctt ggtgatcgag gcgcgcctgg acaagggcaa aggcccggtg
      601 gcgaccgtgc tggttcagtc cggcaccctg cgtcgcggcg actcggtgct ggtgggatcg
      661 gtattcggtc gtgtacgtgc catgctggac gaggccggaa aaaccatcac cgaggctggc
      721 ccgtccattc cggtcgagat tcaaggcttg tccgaagtgc cgggtgcagg cgaagagatg
      781 atggtgatgg cggacgaacg ccgcgctcgc gagatcgcgc tgttccgtca aggcaagtac
      841 cgtggtgtga agctggccaa gcaacaggcc gccaagctgg aaaacatctt cgagcaaatg
      901 ggcgagggcg aggtaagcac cctgtcgctg atcgtcaaat cggacgtgca gggttctgcc
      961 gaagcgatcg cacattcgct gctgaaactc tccaccaacg aggtgaaggt caacctgatc
     1021 cacaatgcgg tgggcgcgat ctccgagtcg gatatcaatc tggctctggc atccaaagcg
     1081 atcgtgatcg gctttaatac ccgtgccgat gccgccgcgc gcaagctggc cgagagcgcc
     1141 ggcgtggaca ttcgctacta caccatcatt tacgcgatga tcgacgagat caaggcggcg
     1201 atgtcgggca tgctggcacc ggagaagcgc gaaagcgtca tcggtatggt cgagatccgt
     1261 caggtcttca cggtctcgaa gatcggttcg atcgccggtt gttacgtgct ggaaggcatc
     1321 gtcaagcgca atgccaaggt acgcgtgttg cgcaacaacg tggtgatcca cgacggcgag
     1381 ctggattctc tgaaacgctt caaggatgac gcgaaggaag tcaaggctgg cttcgagtgc
     1441 ggtctgtcgc tgaagggtta caacgatatc gaagtcggtg acaagctcga agtgtacgag
     1501 atcatcgagg ttgcccgcgc gctgctctga tggccaactt tgccagaaca gatcgaatcg
     1561 gacagcagat ccagcgcgag ctggccgagt tggtgcgact cgaaatcagc gatccgcgcg
     1621 tgcggctggt gacgatcacg ggtgtcgaag tcgccaacga ctattcgcat gccaagattt
     1681 tctttacccg tctggacggc aagcaggaag aggcgcaaca agggctggag cgagcaagcg
     1741 gatttttgcg cagcaagctc gcgcgcagtc tgaagctgcg catcatgccg caattgcatt
     1801 tcgtctttga cgcttccgtc gagcgcggca gccatctgtc gcaactcatc gatcaggcgg
     1861 tcgccagcga tcagcagctc gctgacaaga agtaaactcg atggcacgga cctttcgccc
     1921 gctggatggc gtcctgctat tcgataagcc gctcgaactg agttccaata ccgcgctgca
     1981 aaaggtgcgt cgtttgttcc aggcagagaa agccgggcat accggcacgc tggatccgct
     2041 tgcgaccggc ttgttgccga tctgcttcgg ggaggcgacc aagttttcaa ccgcattgct
     2101 ggatgccgac aagacctatc gcgcgacgct gcgtctgggg cagaccacca ccacgggcga
     2161 tgccgaaggt gagatcgtca gcgaatctcc ggttgtcgtg gttcaggcag acatcgatgc
     2221 cgcgctggag aaatttcgcg gcgagatccg gcagcttccg ccgatgcaca gtgccctcaa
     2281 gcatctgggc aaaccgctgt acgaatatat ccgcaagggc gagaccgtcg agcgggagtt
     2341 gcgcaatgtg gtgatccacg aactgcgcat ggagcgcttc gagggtaatc agctggacat
     2401 cacggtgcgc tgtagcaagg gcacctatgt gcgtacgctg gccgaagata tcggtgccgc
     2461 actgggatgc ggcgcgcatc tgatcgcgct gcgccgtact gcgatcgctc atttcgatct
     2521 ttgcgatgcg tgcgatttgc aacaattaga ggcaatgagt caagaacagc gcgacgcccg
     2581 tgtgttgccg ctcgaaagct tgatgccgga tatgccgaga ttgcagctgg atgccgttca
     2641 aataaaacgc atggcacagg ggcaacggct aggaatggat accgccttac ccaatggaaa
     2701 ggttggtctg tatggctcac aggggtttat tggtgtcgga atgctggatg gccgacgcct
     2761 tgctccggag agactgttgt ctagtgtagc gaagcaggcc gcgaggcccg atttagagca
     2821 aaatattagt gttgagcaat aaccccgttc aggggtaaaa tgccgcactt tttttaggag
     2881 agcaaaacca atggcaatta ccgccgcgca aaaagcgcaa atcgtttcag acttccagcg
     2941 tgcaaagagc gacaccggtt ctccggaagt tcaagtggcg ctgatcaccg cacgtatcgt
     3001 ttatctgacc gagcacttca aagataacgc caaggatcac cattcccgcc gcggtctgct
     3061 gcgtctggta agccgtcgtc gcaagctgct cgactatctg aagagccaga atgatgcagc
     3121 gtaccgttcg ctgatcgaac gcctcaatat ccgtaagtaa gcatggtttc aaacgggtcg
     3181 ctgatgcggc ccgtgtcatt ttgtccagtc aattcgatgg cgcggttccg cgtcatgtca
     3241 tgcataaagg ggagacacct tgagtcacta caaaaaaaca atcgcctatg gaaatcacca
     3301 gctcacgctg gaaaccggtg aaatcgcgcg tcaagccagt ggtgcagtca tggttagtct
     3361 ggatgacacc gtggtgctgg tcaccgtggt ggggcgcaag gatgcaaagc ccgagcagga
     3421 tttcttcccg ctgacagtcg attatcagga aagaacctat gctgcgggca agatccccgg
     3481 cggcttcttc aagcgcgagg gacgtccgag cgaaaaggaa atcctgacca gccgtctgat
     3541 cgatcgtccg ctgcgtccgc tgttccctga gagtttttat aacgaggtgc agatcgttgc
     3601 gaccgtgatg tcgtccgatg ccgagatcga ttccgacatc cccgcgatga tcggtgcgtc
     3661 cgctgcgctg gcgttgtccg gcattccgtt cgacggcccg atcggcgcgg cgcgcgtggg
     3721 ctatgccgat ggcaagtatc tgctgaatcc gaccgcgacc gagctgaaga cttcgctgat
     3781 ggatctggtc gtggccggta ccgaccgtgc cgtgcagatg gtcgaatccg aggcacagca
     3841 actgtccgaa gaggtgatgc tgggtgcggt ggtgttcggt catgagcaga tgcaggtcgt
     3901 gattcaggcc atcatcgaaa tggccgatgc agtcggtgcg ccggcatggg attgggtcgc
     3961 tcctgccaag gacgaagcgc tgatcgcgca aatcaccgcc atcgccgagg ccgagttgca
     4021 gcaggcttac gcaatccgct ccaagcaggc ccgcaccgat gcggttgccg cgattcagag
     4081 caaagtcatg gcagccgtgg ttaccccgga atcggctcct ggccagaaga atcacgtcaa
     4141 tgatctgttc agcgcgctgg aagccaagat cgtgcgcggt cagatcctga gcggcgagcc
     4201 ccgcatcgac ggtcgcgaca cccgcaccgt gcgtccgatc accatccgca acggcgtgct
     4261 gccgcgcgtc catggctcct cgctgtttac ccgtggcgag actcaggcca tcgtggtggc
     4321 aaccttgggc agcatgcgcg actcccagaa gatcgacgcg ttgcagggcg agtattccga
     4381 ccgcttcatg ctgcattaca acttccctcc gtactccacc ggcgaaaccg gccgcgtcgg
     4441 cacgccgaag cgccgcgaaa tcggccacgg tcgtctggcc aagcgcgcgc tggttgcggt
     4501 gctgccgtct gaagaggatt tcggctacgc gatgcgcgtg gtctccgaga tcaccgaatc
     4561 gaacggttcc agctcgatgg cttcggtctg cggcggttgc ctgtcgctgc tggatgccgg
     4621 cgtgccgctg aaggcacacg tggccggtat cgccatgggc ctgatcaagg aaggtaaccg
     4681 tttcgcggtg ctgaccgaca tcctcggcga cgaggatcat ctgggcgaca tggacttcaa
     4741 ggtggcgggt accgaggctg gtgtgaccgc gctgcagatg gacatcaaga tcaacggtat
     4801 caccaaggag atcatgcagg ctgcgctggt tcaggcgcgc gaaggccgca tgcatatcct
     4861 cggcctgatg aagcaggcga tgccggaggc gcgcaccgaa gtttccgatt tcgcaccgcg
     4921 catgatcaag atgaagatca atccggagaa gatccgcgac gtgatcggca agggcggtgc
     4981 ggtcatccgt acgctgaccg aagagaccgg cacgaccatc gatatcgacg acgcaggtaa
     5041 tatcacgatc tcctgcgtga gcggcgaggc cggcgagttg gccaagaagc gcatcgagga
     5101 catcgtggcc gaggtcgaag tcggcaagat ctacgaaggc ccggtggtca aactgctgga
     5161 cttcggtgcg ctgatcaaca tcatgccggg caaggacggc ttgctgcaca tctcccagat
     5221 cgcccacgag cgcgtcaatg ccgtgaccga ccacctcaag gaaggtcaga tcgtcaaggt
     5281 caaggtgctc gaagtggacg acaagggccg catgaagctg agcatgaaag tgctgctgcc
     5341 agcaccggaa gctgcacctg ctgccacacc cgcagccgaa taagttttat tcggtagcaa
     5401 aacgaaaagc cccgccaatc tggcggggct ttttgcttag ggggtggatt tttaagatgg
     5461 ctatttatcg gccattgccg acggtggaag ccagatggct cagtggccgg taaggatacg
     5521 tcaccagcca ttggcgcaga ggagcacctc gaacggcagc tttctcgaaa ttcgactgcg
     5581 tactatcgac cctgagccga ccatcagggt attgaatctg aacgactgct gtaatgctga
     5641 cagctgaccg tcaaaccaag gaaaaatcgt gaatctgaat gcctgttgat aacccgtttg
     5701 cgacagttgg atacgcaaat tcgcttccca ataccggacg atcggcaaac acagctgacg
     5761 ataccgcctc tttagtacag caaggcaaga ccgttcatcg gccttgcggc tgtccaattc
     5821 actccagcca tccttggaaa attcgctgca gcggataatc tgaccagagc agcataaact
     5881 tgatgcttgg ctagccatta tttactccca gtatgcatgg ggaattatct ttcatgcgtt
     5941 ggggactttt aattcgacgg tatgctcatg gcgatcatct atcaaaggaa ccgtttgctc
     6001 cggcaatatt ttcccgtcga gtgaaagctg attcgcgtct gccgcatcca tatcaagccg
     6061 ggtgatcgtg atgt
//
LOCUS       DASUIV010000283         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_73717, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000283 DASUIV010000000
VERSION     DASUIV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73717"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFW59_10170"
     CDS             complement(<1..377)
                     /locus_tag="VFW59_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5364621.1"
                     /translation="MNNIWNERYAGEDYHFGTEPNAFLAAQHQLLKPGMNCLALADGE
                     GRNGVWLAGQGLQVLAVDSSPVALDKARRLARQRGVVLEYELADLSAWAWPEQRFDVV
                     AAIFIQFAPPGLREQMFAHIERSL"
     gene            complement(370..1200)
                     /locus_tag="VFW59_10175"
     CDS             complement(370..1200)
                     /locus_tag="VFW59_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364622.1"
                     /translation="MIKLFVLILSGALLAGCGSPPQRLPQALERAQTADKDARRALRT
                     GDLADARLLFAESLALHRSVDDVDGAASALISLATVSHWLHDDAAAIKLLDQISLDQA
                     SPYSPPWRITAAFRKAVILADLGRNDEAASVLAVADRVCEGNCPLRFGIEVLKARLAL
                     LNGDALAALELAKPVASAREVGDEERANALRLVAAAEEKLTQHQAALRHYRAALELDK
                     LLGLSSRIEQDLNGIVRVLTDLGRVEEAALHARRAFLVHEAARKKASSAPRLIEENNE
                     "
     gene            complement(1193..2113)
                     /locus_tag="VFW59_10180"
     CDS             complement(1193..2113)
                     /locus_tag="VFW59_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5364623.1"
                     /translation="MTLLADKDERFKNLYGKAGVFLLLAVLGIVLIFVLAGMKKGMFT
                     AKAPIYFVADSGQDLSEGMPVKLSGFKVGTVKNLALDELGRVQVEAGIEQEYLALLTE
                     DAVVRLIKVSMIGGDAILDISRGSVGKKALAAGGKIRFERSGGLEAAAVEVRDRLLPI
                     LDDLHQTLHDPDGDLRQTLKNLREFSGEMRGTRKRLDALLEHADTSMNNDVAPMLHSL
                     QQTTMHAESMAVQLERSLPGLVSKADGTLDNLKQTSDTLRSAVEQSAPQLPGLIGETR
                     DIVEGVSTSWPLKNIMPTPESGPVRMDSHD"
     gene            complement(2121..2735)
                     /locus_tag="VFW59_10185"
     CDS             complement(2121..2735)
                     /locus_tag="VFW59_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364624.1"
                     /translation="MTVLFQFSGVACAELNRAYSFSLHAGEIRLLQVVSAAEKEAVID
                     AVLGNGKCAEGAVLLQGVPLGEIKLGSIGWVAANGGLISNLKVWENVTLPLWYHAGHE
                     PGETEQVIASWLSALGMAESEWVGFMESPPGRLLSWQRKLAGLLRGLVQMPPMLVVDA
                     ALFEAVELHRAAAWMAALATYAEQGRAVLVVAAGEAALPWKIIE"
     gene            complement(2737..3507)
                     /locus_tag="VFW59_10190"
     CDS             complement(2737..3507)
                     /locus_tag="VFW59_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5364625.1"
                     /translation="MEYSLQGLQRWLGSQVLFVREGLALTWQAFCGLPAIAKFPVRQS
                     LFKQLYFSGIQSLGSVGAIGILFGLILVIQVHNLVGHNELLMLHVLIWAVVRELGPLL
                     AAIVIIGRSSSAIASELASMQVNGEIDSLRRMAISPVAYMVMPRILGVTLTSLALAFY
                     FQLVAIGTGMLVIAWMLDIALMSEIINFFDMITFLEMAAALLKSLCFGIIVSAVSCHY
                     GLAIERALTEIPKAASRAVIRSLLAVFACDGVITMLLF"
     gene            complement(3632..4537)
                     /locus_tag="VFW59_10195"
     CDS             complement(3632..4537)
                     /locus_tag="VFW59_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX5364626.1"
                     /translation="MLNLILLAVCFAAGIVLRRFGRMPEQAPLTLNSFIIHVSLPALT
                     LLYIHDLKVSGEVLLVAAMAWLVFGLSAGFFWLVGRRLKLPRATVGALMLTGGLGNTS
                     FFGLPMVEAYYGSGALGTAIIADQLGSFFVLSILGITVAGIYSSGRPSAGEILRRIAK
                     FPPFIALLIALLLIPVDYAQWFTLLLKRLGDTLAPLALLSVGLQLRLGHVAEHGRNLA
                     IGLAFKLILAPLMIFLLYVEVFGAQGQTIQVTLFEAAMPPMITAAIIAGEHDLDPPLA
                     NLMVAAGLLLSFFTLSGWWWMMQGV"
     gene            4625..5110
                     /locus_tag="VFW59_10200"
     CDS             4625..5110
                     /locus_tag="VFW59_10200"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5364627.1"
                     /translation="MTSDSNFDIFGSNDSEAPLCDAANLSSLLSDAAPPAIALPKGGK
                     ELRAAQRYIVKWRVTAFIDHRGLHHGVIKDISTKGTAILFEQNFQSVEFIKLHIYVTP
                     PAPSKIPCVIEVLGRIIYAIHDPKEHRFRTGVNFLKFSTEHDPAFLADHLDNHAMTVI
                     G"
     gene            complement(5162..5824)
                     /locus_tag="VFW59_10205"
     CDS             complement(5162..5824)
                     /locus_tag="VFW59_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5364628.1"
                     /translation="MQFSADRKARVMIVDDHSLVREGMAMLINCEPDMQVCCAADQVE
                     QAINASRSCAHDIAIVDMTLGAGSGLELVRRLQFEFPDLPVLMLSMHDESIYAEPALK
                     AGARGYLMKQAATSVLLQAMRQVLNGDLYVSDQLRSRMLKKMRLGAVNDTSINSLTPS
                     ELEVLHLIGMGMGTSEISEKLARSVKTVESHKANIKKKLDLENGNQLTLFAINFVSAG
                     KM"
     gene            complement(5838..>6199)
                     /locus_tag="VFW59_10210"
     CDS             complement(5838..>6199)
                     /locus_tag="VFW59_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364629.1"
                     /translation="FAEHVEGMYHISCEFFHDEHCRVDDQEVATHLFRITQEAVNNAV
                     RHGCATSIRLRMSLSPSTQVLEILDNGCGLLPGATVTGLGLRSMRYRADLIGASMDVS
                     ALPEGGTRVVISMPIEL"
BASE COUNT         1407 a         2012 c         1604 g         1176 t
ORIGIN      
        1 agactgcgtt cgatatgggc gaacatctgc tcgcgcaaac cgggcggcgc gaactggatg
       61 aagatcgccg ccaccacatc gaagcgctgc tcgggccacg cccatgcgga gaggtcggcc
      121 agttcgtact ccagcacgac gccgcgctgc cgcgccagcc gccgcgcctt gtccagcgcc
      181 acgggcgaag aatccaccgc cagcacctgc aacccttgcc cggccagcca cacgccgttg
      241 cggccttcgc cgtccgccag cgcgagacag ttcatgccgg gcttgagcaa ctgatgctgt
      301 gcggccagaa aggcattcgg ttcggtgccg aaatggtaat cctcgcccgc gtagcgttcg
      361 ttccagatgt tattcattgt tctcttcaat caatcgggga gcggacgaag ccttcttgcg
      421 cgccgcttca tgcacgagga aagcgcgccg ggcatggagt gcggcttcct cgactcgccc
      481 cagatcggtc aagacgcgca cgatgccgtt cagatcctgt tctattcgcg aactcaggcc
      541 cagcaactta tcgagttcga gtgcggcacg gtaatgccgc aaggcggcct gatgctgcgt
      601 caatttctct tctgcggcgg cgacaagccg caaggcgttg gcccgctcct cgtcgccgac
      661 ttcccgggct gaagcgacag gcttggccaa ttccagtgcg gcgagcgcat cgccattgag
      721 caaggcaaga cgcgccttca acacctcgat gccaaagcgc aacgggcaat tgccttcaca
      781 cacccgatcc gcaacggcca gaaccgacgc ggcttcatcg tttcgtccga ggtcggcgag
      841 tatcaccgcc ttgcggaagg ctgcggtaat ccgccacgga ggcgaatacg gcgaggcctg
      901 gtctagcgat atctgatcga gcagtttgat cgccgcagca tcgtcatgca gccagtgaga
      961 caccgtcgcc aggctgatca gggcgctcgc cgcaccgtcg acatcatcca ccgaccgatg
     1021 cagcgccaga gattcggcaa acagcagccg cgcatccgcg aggtctccgg tccgcaatgc
     1081 gcgccgggca tccttgtctg ccgtctgcgc gcgctccagt gcctgcggca aacgctgtgg
     1141 cgggctgccg catccggcca acaacgcccc cgacaggatc agcacgaaca gtttaatcat
     1201 ggctatccat cctgaccgga ccgctttccg gtgtcggcat aatatttttc agcggccagc
     1261 tcgtgctcac cccctccacg atgtcgcgcg tttcccctat caatcccggc aactgaggtg
     1321 cagattgctc cactgcgctc ctgagcgtat cgctggtctg cttcaggttg tccagcgtgc
     1381 cgtccgcctt gctcaccagc cccggcaaag accgttcaag ctggactgcc atactctcgg
     1441 cgtgcatcgt cgtttgctgc aacgagtgca acatcggcgc cacgtcgttg ttcatgctgg
     1501 tatccgcatg ttcgagcaaa gcatcgagac gcttgcgggt gccgcgcatt tcgccagaaa
     1561 attcgcgtag attcttgagg gtctggcgca agtcaccatc aggatcatgc aatgtttgat
     1621 gcagatcatc cagaatcggc aacaagcggt cgcgcacctc gacagcggcc gcttccagcc
     1681 cacccgaccg ctcaaaacgg attttgccgc cggccgccaa cgcctttttc ccgactgagc
     1741 cacggctgat gtccaggatt gcgtcgccgc cgatcatgct gacttttatc agcctcacca
     1801 cggcatcttc ggtgagcagc gcaagatatt cttgctcgat tcccgcctcg acctgaacac
     1861 ggccaagctc atccaaagca aggttcttga cggtaccgac cttgaatccg ctcagcttta
     1921 ccggcatgcc ttcactcagg tcctgcccgc tgtccgcgac aaaataaatc ggcgcttttg
     1981 cggtgaacat gcccttcttc atccccgcca acacaaagat cagcacaatg cccaataccg
     2041 ccagcaacag aaatacgcca gccttgccgt acaggttttt aaagcgctcg tccttgtccg
     2101 ccaataacgt cataccgacc tcactcgatg attttccagg gcaatgcggc ctcccccgct
     2161 gccacgacca gcactgcccg cccctgttca gcataggtcg ccaatgcggc catccaggca
     2221 gcggcccggt gcagttcgac ggcttcaaac agcgcggcat ccacgaccag catcggcggc
     2281 atttgcacca gcccacgcaa cagaccggcc aatttccgct gccaggatag caatctgccc
     2341 ggtggagatt ccataaatcc cacccattcc gattcagcca ttcccagcgc cgacagccag
     2401 gatgcaatta cctgttccgt ttcgccgggc tcatgtccgg cgtgatacca caagggcaag
     2461 gttacgtttt cccaaacctt cagattgctg atcagtccgc cattggcggc gacccagccg
     2521 atgctgccca gttttatttc gcccaatggt acgccttgca gcagcacggc gccttcggcg
     2581 catttcccat tacccaggac cgcatctatc acggcctcct tctcggctgc cgagacgacc
     2641 tgaagcaggc ggatctcgcc agcatgcaag gaaaaggaat aggccctatt cagctctgcg
     2701 cacgccacgc cggaaaattg aaaaaggacg gtcatgtcag aacagcagca tcgtaatcac
     2761 gccatcgcat gcaaacacgg ccaataaact gcgtatcacc gcgcgcgagg ccgccttggg
     2821 tatttctgtc agcgcgcgtt cgatcgccag tccgtagtga caggaaaccg cagacacgat
     2881 gatgccgaaa caaagacttt tcagcagagc ggctgccatc tcgagaaagg tgatcatgtc
     2941 gaagaagttg atgatctcgc tcatcaacgc aatgtcgagc atccaggcaa ttaccagcat
     3001 gccggtgccg atcgccacca gctgaaaata gaaagccagc gccagactgg tgagggtcac
     3061 gcccaatatt ctcggcatga ccatgtaagc aacgggcgag atcgccatgc gccgcaagga
     3121 gtcgatctca ccattgacct gcatcgaggc aagttcgctg gcaatggccg aactgctgcg
     3181 accgataatg acaattgcgg ccagcaacgg ccccagttca cgcacaacgg cccagatcag
     3241 cacatgcaac attaacaact cgttatgtcc aaccaggttg tgaacctgga tcaccaggat
     3301 cagtccgaac agaatcccga tcgcgccaac cgaacccagg gactggatac cgctgaaata
     3361 caactgtttg aacaaggact ggcgtaccgg gaatttcgct atcgcgggca ggccacaaaa
     3421 agcctgccac gtcagtgcca gcccctctcg aacaaacagc acctgactgc ccagccagcg
     3481 ttgcaatcct tgcagagagt attccatgtg tgccctcgca tgccacggct caaattaaat
     3541 atgccaccca ccgccacgca gtcggcgcac aaggataata gcagccccgg cgccaccctc
     3601 tccagcaaag cggacttggg tctcgcgatg cctatacgcc ctgcatcatc caccaccagc
     3661 cgctgagggt aaaaaacgac agcaggagac ccgcagccac catcagattc gccagcggcg
     3721 ggtcgagatc gtgctcgccc gcgatgatgg cggcggtgat catcggcggc atcgccgcct
     3781 cgaacagcgt cacctgtatg gtctgtccct gcgcgccgaa cacctccaca tacagcagaa
     3841 aaatcattag cggcgcgagg atgagcttga aggccagacc gatcgccaga ttgcggccat
     3901 gctccgccac atgccccagc cgcagctgca aaccgacaga cagcagcgcc agcggtgcga
     3961 gcgtgtcgcc caaccgtttg agcaacagcg tgaaccattg tgcatagtcg accgggatca
     4021 gcaacaaggc gatcagcaag gcgatgaagg gcgggaattt tgcgatgcgt ctcaagatct
     4081 cgcccgcgct ggggcgaccg gaggaataga tccccgcgac ggtgatgccg aggatggaca
     4141 gcacgaagaa cgagccgagc tgatcggcga tgatcgcggt gcccagcgca cccgagccgt
     4201 aataggcttc gaccatgggc agaccgaaga acgaggtgtt gcccaatccc ccggtcagca
     4261 tcaaggcccc caccgtggca cgcggcagct tcagcctgcg ccccaccagc cagaagaacc
     4321 ctgccgacaa cccgaacacc agccaggcca tcgcggccac cagcaacacc tcgccggaga
     4381 ctttcagatc gtggatatac aacagcgtca gcgcgggcag cgaaacgtgg ataatgaagc
     4441 tgttcagcgt gaggggggcc tgctccggca tacggccgaa gcggcgcaga acgatccccg
     4501 ccgcgaaaca gacggcaagc aaaatcagat tcagcatttc aagcaggttc ggataaaatc
     4561 catcatgtta gtatttacaa tatctaattc ctgaattcac gtttcctccg aagacactcc
     4621 atccatgacc tcagattcga attttgacat ttttggcagc aacgacagcg aggctccgct
     4681 gtgcgatgcg gcgaacctgt catcgttgct gtcggatgct gcgccaccgg ctatcgctct
     4741 tcccaaaggc ggaaaagaac tccgtgcggc acagcgctat atcgtcaagt ggcgagtcac
     4801 cgccttcatc gaccaccggg ggctgcacca cggcgtcatc aaggacatct ccaccaaggg
     4861 gacggcgatt ctgttcgagc agaacttcca gtccgtcgaa ttcatcaaac tgcacatcta
     4921 cgtgacacca ccggcacctt caaagatacc ctgcgtgatc gaagtgctgg gaaggatcat
     4981 ctacgccatt cacgacccca aggagcatcg cttcaggacg ggcgtcaatt ttttgaaatt
     5041 cagcacggaa cacgatcccg cattcctagc cgatcacctc gataatcatg cgatgacggt
     5101 catcggctga cccctcgctt cccgaacccg cctctcaccc cctgccgtga aggcacgggg
     5161 atcacatctt gccagcggag acaaagttga ttgcgaacaa tgtcaattga ttgccattct
     5221 ccagatcaag tttcttcttg atattggcct tgtgcgactc gaccgtcttg acgctgcgcg
     5281 ccagtttttc agagatttcg ctggttccca tgcccatacc gatgagatgc aacacctcca
     5341 gttcgctggg ggtaaggctg ttaatcgatg tgtcattgac ggcaccgagc cgcatttttt
     5401 tcagcatacg ggatcgcaac tgatcgctca catacaagtc tccgttcaat acctgacgca
     5461 tcgcctgcaa cagtacgctc gtcgccgcct gtttcatcag gtagcctcgc gcgcctgctt
     5521 tcaatgcagg ctcggcataa atcgactcat catgcatgga gagcatcagc accggcagat
     5581 cggggaattc gaactgcaaa cgacgcacca gctccaatcc ggatcccgcc ccaagggtca
     5641 tatcgacgat ggcgatatca tgcgcacagg aacggctggc attgatcgcc tgctcgacct
     5701 gatccgccgc gcagcagacc tgcatgtccg gctcgcaatt gatcaacatc gccatcccct
     5761 ccctcaccaa ggaatggtca tcgacgatca tgactcgggc ttttctatct gcgctaaatt
     5821 gcatattttc caaaatgtta taattcgatc ggcatgctga tcaccaccct tgtcccacct
     5881 tccggcagcg ccgaaacatc catggaagca ccaatcagat ccgcacgata acgcatggag
     5941 cgcaaaccta accccgtcac cgtcgcacca ggaagcagcc cgcaaccatt gtcgaggatt
     6001 tccagcacct gcgttgacgg agagagcgac atacgcagcc ggatactggt ggcgcatcca
     6061 tgcctgaccg cattgtttac cgcttcctga gtgatgcgaa acaaatgagt ggcaacctct
     6121 tggtcatcta cccggcaatg ttcgtcatga aaaaactcac agctgatatg gtacataccc
     6181 tccacatgtt cagcgaatt
//
LOCUS       DASUIV010000284         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1375470, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000284 DASUIV010000000
VERSION     DASUIV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375470"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3494
                     /locus_tag="VFW59_10215"
     CDS             <1..3494
                     /locus_tag="VFW59_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5364630.1"
                     /translation="LFNLTHRGDIGADALFAEHCRFGAQFEVPSQAFANPREPERCLR
                     IGIVSGDLYLHAVANFIEPVLMRLANSAQLSLHAYYNHVQEDEVTQRLKGHFAQWGSV
                     FGLTDEELAQKIRADGIDILIDLSGHTARNRLPVFARKPAPLQASWMGYPGTTGLQAM
                     DYYLGDRFWLPPGQFDHQFTEKIVRLPANAPFLPAGGAPDINALPALENGYVTLGSFN
                     RPNKISQPVVALWSRLLQALPESRLLLGAMPKDGENDQLITWFAQEGIEQERLTLHPR
                     SGMDVYLALHQQVDICLDTFPYNGGTTTCHAAWMGVPTLTLAGTTVTGRVGAMIAGHL
                     GLDEFVAQDPDDFVRKGVYWAGHLTELAELRAGMRARFEQSALGRPELIAAGLEQALR
                     TMWRRWCNGLPAESFEVEAGVSPAEVLRHAAAFYQAGRLAEAEELCLSILQTEPGHAD
                     ANYNLGLMALQQGQAERALPYLQAAWEGAPGEGAYWLTLTECLLALARFEDALGLIEE
                     AIRRGMDTPQAQQLRLLAQRGRNRAAPDRAVAEGMQALFEAGRYAEMEQQARSLLAQY
                     PGWALGWKALGAALQSQDKDAESILRRAVEISPRDAEAHNNLGVYLRKHGRPSEAVAS
                     LQAAVQIQPTFAEAHFRLGGALADLREFDKALESFRCAAKLRDGYAEAYQSMGAVLNG
                     LGRFDEAVASYRLALEIKPDYVDARVNMGSALLGQGKISEAKASYRQALELMPGSAEL
                     YSNYLFCLSHDDEGGAETIASEHRKFGERFETPLHEEWGGYANIPDPERRLHVGLVSA
                     DLFNHAVAGFIEPVLACLADHSNMVLHAYSNTSVEDEVTKRLKGYFAHWDGVAGMADE
                     ALARKIRADGIDILIDLSGHTAHNRLLTFARKPAPLQASWMGYPGTTGLQAMDYYFGD
                     RFFLPTGQFDDQFMEKIVRLPANAPFLPAADAPTVNVLPALENGYLTFGSFNRPSKLS
                     RPVIALWSRLLRALPDSRILLGAMSRDGTYGELLDWFAQEGIARERLSFHPRTGMDAY
                     LALHQQVDICLDTFPYNGGTTTCHAAWMGVPTLTLAGETGAGRVGVMILGHLGLGEFA
                     AHTPDDFAQKAVYWAGHLAELAELRAGMRARFEGSALGRPELIAAGLEQALRIMWRRW
                     CAGLPAESFEVPGAGEG"
BASE COUNT          659 a          938 c         1249 g          752 t
ORIGIN      
        1 tgctgtttaa tctgacccat cgtggtgaca tcggcgcaga tgcactcttt gccgagcact
       61 gccgcttcgg agcgcagttc gaggtgccat cgcaggcatt cgccaatccg cgcgaaccgg
      121 agcgctgtct gcggatcggc atcgtgtcag gcgatctgta tctccacgcg gtcgcaaact
      181 tcatcgagcc ggtattgatg cgtctggcga atagcgcgca attgtcgctg catgcctatt
      241 acaaccatgt acaggaagac gaggtcacgc agcgtctgaa ggggcacttt gcgcagtggg
      301 gctcggtgtt cggtctgacg gacgaagagc tggcacagaa gatccgtgcg gatggcatcg
      361 acatcctgat cgatctctcc ggccacacgg ctcgcaaccg attgccagta tttgcgcgca
      421 agccagcgcc gttacaggcg agctggatgg gctaccccgg caccacgggt ttgcaggcga
      481 tggattatta tctgggcgat cgattctggt tgccaccggg tcagttcgat catcagttca
      541 cggaaaagat cgtacgtctg ccggccaatg cgcccttcct tcctgcgggt ggagcgccgg
      601 atatcaatgc gttgcctgcg ctggagaatg gctatgtgac cttgggcagt ttcaatcgcc
      661 caaacaagat cagtcagcct gtagttgcgc tgtggtcgag gctgttgcag gcgctgcctg
      721 agtcaaggct gttgcttgga gcgatgccta aggatggcga gaatgatcaa ttgatcacct
      781 ggtttgctca ggaaggcatt gagcaagagc gtctgaccct tcatccccgc tccggcatgg
      841 atgtctatct ggcattgcat cagcaggtgg acatttgcct ggataccttc ccgtataacg
      901 gcggcaccac gacctgccat gcggcatgga tgggggtgcc gacgttgacg ctggcgggca
      961 cgacggtgac agggcgggta ggtgcgatga tcgcgggtca cctcgggttg gatgaattcg
     1021 ttgcacagga tccggacgac ttcgtgcgta aaggcgtgta ttgggccggt catctgaccg
     1081 agctggcgga gttgcgcgcc gggatgcgtg cgcgattcga gcaatcggcg ctggggcggc
     1141 cggagctgat cgcggccggt ctggagcagg cgttgcgcac catgtggcgg cgctggtgca
     1201 atggactgcc agcggaatcg ttcgaagtgg aagctggcgt tagcccggcg gaagtgttgc
     1261 ggcatgcggc cgcgttctac caggcaggac ggctcgccga ggcggaggaa ttgtgtctga
     1321 gcatcctgca gacggagccc ggacatgcgg acgcgaatta caacctcggt ctgatggcgc
     1381 tgcagcaggg gcaggcggaa cgcgctttgc cgtatttgca ggcagcctgg gaaggcgcgc
     1441 ccggggaggg tgcgtactgg ctgacgttga cggaatgcct gctggcgctg gcgcgcttcg
     1501 aggatgcgct gggtttgatc gaggaagcga tcaggcgcgg tatggacacg ccgcaggcac
     1561 agcagctgag actgctcgct cagcgcggaa ggaaccgggc ggcgccggac agggctgtcg
     1621 ccgagggcat gcaggcgctg ttcgaggcgg ggcgttacgc cgaaatggag cagcaggcga
     1681 ggtcgttgct cgcacagtat ccgggctggg cgctgggctg gaaagcgttg ggggcggcgt
     1741 tgcagtcgca ggacaaggac gccgagtcca ttttgcgccg tgccgtcgag atctcgccgc
     1801 gcgatgccga ggcgcataac aatctggggg tgtacctgcg caagcatgga cgaccaagcg
     1861 aggcggtggc gagtttgcag gccgccgtac aaattcagcc gacatttgcc gaggcgcatt
     1921 tccggctcgg gggggccttg gccgatctgc gcgagttcga caaggcgctg gaaagttttc
     1981 gatgtgcggc gaaactccgg gatggatatg ccgaggcgta ccagagtatg ggcgcggttc
     2041 tgaatggctt gggacgattc gatgaggccg tggccagtta tcggctggca ctggaaatca
     2101 agccggatta tgtggatgca cgggtcaata tgggcagtgc cctgctcggt caaggcaaga
     2161 tatccgaggc caaggcaagt taccggcagg cgctggaact catgcccgga tcggccgagc
     2221 tatacagcaa ttacctgttt tgtctcagtc acgacgatga aggtggtgcg gaaacgattg
     2281 cctccgaaca ccgcaagttc ggcgagcgtt ttgaaacgcc attgcacgag gagtggggcg
     2341 ggtatgccaa tattccggat cccgagcgcc gtctgcatgt tgggcttgtc tctgcggacc
     2401 tgttcaatca tgcggtagcc ggttttatcg agccggttct ggcctgtctt gcggaccatt
     2461 caaacatggt gttgcatgct tattcgaaca cgtccgtgga agatgaggtc acgaagcgtt
     2521 tgaagggcta ttttgcgcat tgggatggcg ttgccggtat ggcggatgag gcgctggcac
     2581 gaaaaattcg tgcggacggc atcgatatat tgatcgacct ttccggacac accgcgcaca
     2641 atcgtctgct gaccttcgcg cgcaagccag cgcccttgca ggccagctgg atgggctatc
     2701 cgggcaccac gggtttacag gcgatggatt attattttgg agatcggttc tttttgccaa
     2761 cggggcaatt cgatgatcag ttcatggaaa agatcgttcg tctgccggcc aatgcgcctt
     2821 ttctgccggc ggccgatgcg ccgacggtca atgtcttgcc cgcgctggag aatggctacc
     2881 tgaccttcgg cagtttcaac cggccgagca aactcagtcg tccggtcatc gcgctctggt
     2941 cgcgactatt gcgtgcgctg cccgattcga gaattttgct cggggcgatg tcgagagacg
     3001 gtacatacgg cgaattgctc gactggttcg cgcaggaggg tatagcgcgc gagcgtctga
     3061 gttttcatcc gcgcaccggc atggatgcct atctggcgct gcaccagcag gtggacatct
     3121 gtctggatac ttttccctat aacggcggca ccacgacctg tcatgcggcg tggatgggtg
     3181 tgccaacgtt gacgctggcg ggcgagactg gggcgggacg agtgggggtg atgatacttg
     3241 gtcaccttgg gctgggtgag ttcgcagcac acactccgga tgattttgca cagaaggctg
     3301 tgtattgggc aggtcacttg gcggagctcg cggagttgcg cgccgggatg cgtgcgcgtt
     3361 tcgaggggtc ggcgctgggg cggccggagc tgatcgcggc ggggctggag caggcgttgc
     3421 gcatcatgtg gcggcgctgg tgtgcggggc tgcctgcgga atcgttcgag gtgccaggtg
     3481 ccggagaggg ataatatgat gcgggagatt agatgatgag cgagtcgaag aaagcgccgg
     3541 tgtatgtgac gcagcccctg ctgcctccgc tggaagattt tgtcccctat ctcgaaca
//
LOCUS       DASUIV010000285         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1003760, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000285 DASUIV010000000
VERSION     DASUIV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003760"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1451)
                     /gene="ccoN"
                     /locus_tag="VFW59_10220"
     CDS             complement(15..1451)
                     /gene="ccoN"
                     /locus_tag="VFW59_10220"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEX5364631.1"
                     /translation="MATVSVAANEQYNYDIVRKFAIMTLIWGAVGMLVGVYIASELAF
                     PFLNFDIPYITFGRLRPVHTGAVIFGFGGSALFATSYYIVQRTCQARLFGGGLSTFTF
                     WGWQLIIVLAALGDVLGYSQGREYAEMAWPTDLLIEVVWVAYMAVFIGTIMKRKQEHI
                     YVANWFYLAFMLATALLHTFNNLAVPISFTSLESYPLFAGAQDAMTQWWYGHNAVGFF
                     LTAAFLGMMYYFVPKQAGRPIYSYRLSIVHFWALIFLYMWAGTHHLHWTAIPDWTSTL
                     AATFSIMLLLPSWGGMINGIMTLSGAWDKLRTDPIMRFLIVSLSFYGMSTFEGPMMSL
                     KDVNALSHYTDWTVGHVHSGALGWVAMISFGAMYHMIPRLWNTQMFSNKLINVHFWLA
                     TIGILLYITALWISGIMQGLMWRAFDDFGNLQYSFVESVAAMHPYYVMRALGGLFFLS
                     GMLVMCYNIFKTIQQGNTAPVNTAAAAA"
     gene            complement(1602..2186)
                     /locus_tag="VFW59_10225"
     CDS             complement(1602..2186)
                     /locus_tag="VFW59_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5364632.1"
                     /translation="MRWWWLWLGLLLGCSPDAARNGEHFVGSVVDGGHVGSTAVLAGH
                     DGKPHRLADFRGKAVLLFFGYTHCPDVCPTAMAELNRAMLLLGARAKEVQVLFVTLDP
                     ERDTAAVLSRYVPYFNPAFIGLRGDAEVTKQLARDFKAFYARQASDSRSGYTIDHEAG
                     VYVFDRQGDLRLYMSHGLGGKEIAHDLALLLDEK"
BASE COUNT          640 a          718 c          579 g          414 t
ORIGIN      
        1 ctcaacttat ccggttatgc ggcagcagca gcggtattaa ccggtgccgt attgccctgc
       61 tggatggtct tgaaaatgtt gtagcacatg accaacatgc cggacaggaa gaacaggccg
      121 cccaaggcac gcatcacata gtagggatgc attgcggcga cagattcgac aaacgaatat
      181 tgcaggttgc cgaagtcgtc gaacgcacgc cacatcagac cttgcatgat gccggagatc
      241 cacaatgcgg tgatgtacag caggataccg atcgtcgcca accagaagtg gacgttaatc
      301 agcttgttgc tgaacatctg cgtattccac aggcgcggaa tcatatggta catggcgccg
      361 aaggaaatca ttgccaccca gcccagcgca ccggagtgca cgtgaccgac agtccagtcc
      421 gtgtagtgcg acagcgcatt cacgtccttc agcgacatca tcgggccttc aaacgtggac
      481 ataccgtaga acgacaagga cacgatcagg aatcgcatga tcggatcggt acgcagcttg
      541 tcccaggcac cggacaacgt catgatgccg ttaatcatcc cgccccagga aggcagcaac
      601 agcatgatgg agaacgtagc cgccagcgtg gaagtccagt cagggatggc cgtccagtgc
      661 aggtggtgcg tacccgccca catgtacagg aagatcagcg cccagaagtg gacgatggac
      721 agacgatagg aatagatcgg acgaccggct tgcttgggca cgaagtagta catcatgccg
      781 aggaaagccg cagtcaggaa gaagcccacc gcgttgtggc cgtaccacca ttgcgtcatc
      841 gcatcctgcg cgccggcaaa cagcgggtag gattcgagcg aagtgaagct gataggcact
      901 gccagattgt tgaaagtatg cagcagagca gttgccagca taaatgccag gtagaaccag
      961 ttggcaacgt aaatgtgctc ttgcttgcgc ttcatgatgg tgccgatgaa gacggccatg
     1021 taggcaaccc agaccacttc gatcagcagg tcagtcggcc aagccatttc ggcatactca
     1081 cgaccttggg aataacccag cacatcgccc agtgctgcca gcacgataat gagctgccaa
     1141 ccccagaagg taaaagtcga caggccgcca ccgaacaggc gcgcctgaca ggtacgctgc
     1201 acgatgtaat aggaggttgc aaacagcgcg ctaccaccaa aaccaaaaat cactgccccc
     1261 gtatgcaccg ggcgcaaacg accgaaggtg atatacggaa tatcgaaatt aagaaacgga
     1321 aaagccagtt cagaggcgat atacaccccc accaacatcc ccaccgcacc ccatatcagg
     1381 gtcatgatgg cgaacttccg aacaatatcg taattgtatt gctcgttcgc cgcgacactc
     1441 accgttgcca ttcgctgcct cccttttgta actaaaaaac caaaacactc catcacaccc
     1501 ggagcacgtt taccccctgc gacagaatga cgcattctgc acgcgcaaag tataagtcag
     1561 ccaccttaat cagacaagca ggtcttgttc gataacgcac attatttttc atccagcaat
     1621 aacgccagat catgcgcaat ctccttaccg cccagcccat gactcatata aagccgcaaa
     1681 tccccttgcc gatcaaatac gtaaaccccg gcctcatggt cgatggtata acccgaccgc
     1741 gaatccgacg cctgtcgcgc ataaaacgcc ttgaaatccc gcgccagttg tttcgtgact
     1801 tcagcatcgc cgcgcaagcc gataaaagca ggattgaagt aaggcacgta acgactcaac
     1861 accgccgccg tatcccgctc ggggtcgagc gtgacgaaca acacctggac ctctttcgcg
     1921 cgcgccccca gcaacagcat cgcccgattc agctccgcca tcgccgtagg acacacatct
     1981 gggcaatggg tatagccgaa aaacagcagg accgccttgc ccctgaagtc cgccagccga
     2041 tgcggcttgc catcatggcc cgccagcacc gccgtactgc cgacatgtcc gccatccacc
     2101 accgaaccga cgaaatgctc gccgttccgc gccgcatcgg gagaacaccc cagcaaaagc
     2161 cccaaccaca accaccacca gcgcatactc acaacctctc ggcaaaaaaa atccccgcca
     2221 gtacaaccgg gcggggattt ttcctttcag ggaaatggat tacatccact ccgaagaaat
     2281 tagaacttct tgccgaagcc gacgctaagg acccaaggat caatatccag agtgtcgatc
     2341 ttctgataac c
//
LOCUS       DASUIV010000286         3025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_75060, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000286 DASUIV010000000
VERSION     DASUIV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75060"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFW59_10230"
     CDS             complement(<1..609)
                     /locus_tag="VFW59_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX5364633.1"
                     /translation="MKAPELLLPAGTLDKMRTAYAFGADAVYAGQPRYSLRARNNEFQ
                     LDELQIGIQEAHALGKKLFVASNLMPHNAKVKTYMADMEPVIAMKPDALIMADPGLIA
                     MVRERWPEVDVHLSVQANTVNYAAVKFWKSLGLTRVILSRELSLDEIEEIRQQCPDIE
                     LEVFIHGALCIAYSGRCLLSGYFNHRDANQGTCTNSCRWDYKV"
     gene            complement(770..1600)
                     /gene="metF"
                     /locus_tag="VFW59_10235"
     CDS             complement(770..1600)
                     /gene="metF"
                     /locus_tag="VFW59_10235"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX5364634.1"
                     /translation="MNKPLFSFEFFPPQTAEGMEKLAATRKQLAALHPEFFSVTFGAG
                     GSTQERTMEAVRQIKADGFQVAPHLSCVGSTRENIRNLLQTYQQMGVGRIVALRGDLP
                     SGMASIGEFQYANELVSYIRAQTGEHFHIEVAAYPEFHPQAKSARDDLVNFKRKVTAG
                     ADSAITQYFYNADSYFHFVDECRKLGITIPIVPGIMPIVKYSQLARFSDMCGAEIPRW
                     MRKTLESYGDDNESVQAFGLDVVTELCEKLLAGGAPGLHFYTLNQAGPSMEVWKRLGL
                     "
     gene            complement(1597..1950)
                     /locus_tag="VFW59_10240"
     CDS             complement(1597..1950)
                     /locus_tag="VFW59_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5364635.1"
                     /translation="MRLLLIWILNALALLTVANFVPGIHLDSFAAAMIAAFFLGLVNT
                     LIRPLLLLLTLPVTLLTLGLFIFVINGLLFWFVGSVLKDFVVDGFWYGVLGAVLYSIF
                     SWALSAAAQPLLRKT"
     gene            complement(2032..>3025)
                     /locus_tag="VFW59_10245"
     CDS             complement(2032..>3025)
                     /locus_tag="VFW59_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5364636.1"
                     /translation="TLLLHLGARAEKDATLLNNPGSEEETCLFASIKAKLAADKTWYS
                     TRLAAIKGVTEETTTGVHRLYQMHERGELKFPAINVNDSVTKSKFDNLYGCRESLVDG
                     IKRATDVMIAGKIAVICGYGDVGKGSAQAMRALSAQVWVTEIDPICALQAAMEGFRVV
                     TMEYAADKADIFVTTTGNFHVITHDHMVAMKNNAIVCNIGHFDNEIDVASLEKYQWDE
                     IKPQVDHVIFPDGKRIILLAKGRLVNLGCGTGHPSYVMSSSFANQTLAQIELFVRTAQ
                     YPVGVYTLPKHLDEHVARLQLTTLNAQLSELTDKQAAYIGVPKNGPYKADHYRY"
BASE COUNT          633 a          922 c          880 g          590 t
ORIGIN      
        1 caccttgtaa tcccagcggc aggaattggt gcaggtgccc tgattcgcgt cgcggtggtt
       61 gaagtaaccc gacaacaggc agcgcccgga ataggcgata cacagcgcgc cgtggatgaa
      121 cacctcgagc tcgatgtccg ggcattgctg gcggatctct tcgatctcgt cgagcgacag
      181 ctcgcgcgac agaatcacgc gggtgagccc cagcgacttc cagaacttca ccgccgcata
      241 gttcacggta ttggcctgca ccgacagatg cacgtccacc tccggccagc gctcgcgcac
      301 catcgcgatc aggccgggat cggccatgat cagtgcatcc ggtttcatcg cgatcaccgg
      361 ttccatgtcg gccatatagg tcttcacctt ggcgttgtgc ggcatcaggt tgctggcgac
      421 aaacagcttc ttgcccagcg catgcgcttc ctgaatcccg atctgcagct catccagctg
      481 gaattcgtta ttgcgcgcgc gcagactgta gcgcggctgc ccggcataca ccgcatccgc
      541 gccgaaggcg taagcggtgc gcatcttgtc cagcgtaccg gcaggaagga ggagttcggg
      601 tgctttcatc gatgattcaa tccagaaaca gttcgctcac catcaaatgg ggcgatattt
      661 tgcacaatgc aacaacagaa aaactgatcc actaaaaaca caaaagacac caaatcaacc
      721 catcggattt gtttcgtgtc tttcgcgtct ttcgtggaca aatcgccttt tatagaccca
      781 gccgcttcca gacttccatc gacggcccgg cctgattcag cgtgtagaaa tgcaagcccg
      841 gcgcgccgcc tgccagcagt ttctcgcaca gctcggtgac cacatcgagg ccgaaggcct
      901 gcaccgactc gttgtcgtcg ccgtagctct ccagcgtctt gcgcatccag cgcggaatct
      961 ccgcaccgca catgtcggag aaccgcgcga gctgcgagta cttcacgatc ggcatgatac
     1021 ccggcacgat cgggatggtg atgcccagct tgcggcactc atcgacgaag tggaaatagc
     1081 tgtcggcatt gtagaaatac tgggtgatgg cggaatccgc tcccgccgtc accttgcgct
     1141 tgaaattgac cagatcgtcg cgcgccgatt tggcctgcgg atggaactcg ggataggccg
     1201 cgacttcgat atggaagtgc tcgccggtct gcgcacggat gtaagacacc agctcgttcg
     1261 catactgaaa ctcgccgata ctggccatgc cggacggcag atcgccacgc agcgcgacga
     1321 tacggcccac ccccatctgc tgataggtct gcaacaggtt gcggatgttc tcgcgcgtcg
     1381 aaccgacaca ggacagatgc ggtgcgacct gaaaaccgtc ggccttgatc tgccgcacgg
     1441 cctccatggt gcgctcctgg gtggagccac ccgcaccgaa ggtgacggag aagaactcgg
     1501 gatggagtgc cgccaactgt ttgcgcgtgg cggccagttt ctccatgccc tccgccgttt
     1561 gtggcggaaa aaactcgaaa ctaaaaagtg gtttattcat gttttcctca aaagcggctg
     1621 agccgcagcc gaaagcgccc acgagaagat gctgtacagc accgcgccga gcacgccata
     1681 ccagaagcca tccaccacga agtccttgag caccgagcct acgaaccaga acaacagtcc
     1741 gttgatgaca aagatgaaca ggcccaaagt gagcagcgtg accggcagcg tgagcaacag
     1801 caacagcgga cggatcagcg tgttgaccag cccaagaaag aacgcggcga tcatcgcagc
     1861 ggcaaagctg tccagatgga tacccggaac gaagttcgcc acggtcagta aagccaacgc
     1921 attcaagatc cagatcagca gcagtctcat cggtccctct tcccgaccca catggcaaaa
     1981 cgccgctcca ccggcgagat tcgctgggga gcggcgatgc gatgcagtcg cttagtaacg
     2041 gtagtggtcg gccttgtacg gaccattctt gggcacgccg atgtaagcgg cttgcttgtc
     2101 ggtcagttcg ctcagctgcg cattcagcgt ggtcagttgc agacgggcaa catgctcatc
     2161 cagatgcttg ggcagggtgt acacgccgac cggatattgt gcagtgcgca cgaacagttc
     2221 gatctgcgcc agcgtctggt tcgcgaacga agaggacatc acgtaggaag gatggcccgt
     2281 gccgcaaccc agattcacca gacgaccctt ggccagcagg atgatgcgct tgccgtccgg
     2341 gaagatcacg tgatcgacct gcggcttgat ctcgtcccac tggtattttt ccagcgaggc
     2401 gacgtcgatc tcgttgtcga agtggccgat gttacaaacg atggcgttgt tcttcatcgc
     2461 gaccatgtgg tcatgggtga tcacgtggaa gttaccggtg gtggtcacga agatgtcggc
     2521 cttgtcggcg gcgtattcca tggtcacgac gcggaaaccc tccatcgccg cctgcagcgc
     2581 gcagatcgga tcgatctcgg tcacccagac ttgcgcggag agcgcgcgca tcgcctgagc
     2641 ggaacccttg cccacgtcgc cgtaaccgca gatcaccgct atcttgcccg cgatcatcac
     2701 gtcggtggcg cgcttgatgc cgtccaccag cgactcgcgg cagccataca ggttgtcgaa
     2761 cttggacttg gtcacggagt cgttgacgtt gatcgccggg aacttcagtt cgccgcgctc
     2821 gtgcatctgg tacaggcggt gtacgccggt ggtggtctct tcggtcacgc ccttgatcgc
     2881 ggccagacgg gtggagtacc aggtcttgtc ggccgccagc ttggccttga tcgacgcgaa
     2941 cagacaggtc tcctcttcgg agccgggatt gttcagcagc gttgcatcct tctcggcgcg
     3001 cgcgcccaga tgcagcagca gcgtc
//
LOCUS       DASUIV010000287         7081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_75426, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000287 DASUIV010000000
VERSION     DASUIV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75426"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFW59_10250"
     CDS             complement(<1..766)
                     /locus_tag="VFW59_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX5364637.1"
                     /translation="MSEQQLQLIPFAHIHGEPMMALPQDLYIPPDALELVLETFQGPL
                     DLLLYLIRKHNLDVLDIPMAELTRQYMGYIEMMQQHRLELAAEYLLMAAVLIEIKSRM
                     LLPRPPKVSDEDGEDPRVELMRRLLEYEQMKLAAQQLNELPQAGRDFEIVHVLMETTV
                     KERLPEICVEDLRQAWLGVLARAKLNKHHKVRREQLSVREQMTHVLRCLKGGEYIPFE
                     QLFDLEEGAPKLVVTFIAILELAKEYLLEIQQSEIFG"
     gene            complement(763..984)
                     /locus_tag="VFW59_10255"
     CDS             complement(763..984)
                     /locus_tag="VFW59_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX5364638.1"
                     /translation="MPKLAVLSGRELCAILMLHGFLKVRQRGSHIVMQLKTETSTITV
                     PVPDHDEIRVGTLLSIIRQSGVPRSEFER"
     gene            complement(977..1198)
                     /locus_tag="VFW59_10260"
     CDS             complement(977..1198)
                     /locus_tag="VFW59_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5364639.1"
                     /translation="MNYQFTAVIEREGDGYVSLCPELDVASQGDTVDQARSNLREAVE
                     LFLECAAPEEIHGRLHNEVYVTRLEVAVA"
     gene            complement(1224..2426)
                     /locus_tag="VFW59_10265"
     CDS             complement(1224..2426)
                     /locus_tag="VFW59_10265"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5364640.1"
                     /translation="MFADRVLSGMRPTGNLHLGHYHGVLKNWVRMQHEFECLFFVADW
                     HALTTHYDTPQVIEQSVWDMVVDWLAAGVDPAHATLFIQSRVPEHAELHLLLSMITPL
                     GWLERMPTYKDQQEKLTSKDLSTYGFLGYPLLMSADVLIYRATQVPVGEDQVPHIEFT
                     RELARRFNHIYGREPGFEEKAEAAVKKLGSKKAKLYAELRTRYQQQGDDEALESGKAL
                     LDEQQSLSHGDRERLFGYLEGSGKMILSEPQAMLTAASKMPGLDGQKMSKSYNNTITL
                     REDEASVTKKIRTMPTDPARIRRTDCGTPEKCPVWALHQVYSNDDVKQWVQKGCTTAG
                     IGCIECKQPVIEAVLEEQRPMRERAQLYLDDPALVRNIIADGCEKARKLASETMRDVR
                     EAMGLSYT"
     gene            complement(2434..3081)
                     /locus_tag="VFW59_10270"
     CDS             complement(2434..3081)
                     /locus_tag="VFW59_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5364641.1"
                     /translation="MQVEQLIQTVAIGAIPILFAITLHEAAHGYVARHFGDMTAYAQG
                     RISLNPARHIDPVGTILLPLLTLALGGILFGWAKPVPVNFGALRRPKQDMMWVALAGP
                     ASNLVMALGWALLYKLALVTPDNYFAEPLLEMAGIGIKINAVLMVLNLLPLPPLDGGR
                     VAVSLLPYRQAYQLSRIEPYGMFILIFLAITPVLGWILSPLVGAVYQLLSLLFGL"
     gene            complement(3081..3710)
                     /locus_tag="VFW59_10275"
     CDS             complement(3081..3710)
                     /locus_tag="VFW59_10275"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5364642.1"
                     /translation="MAQFFNIHLDNPNARLIRQAAAVLRDGGIVVYPTDSCYALGCHL
                     DDKEAVARIRQIRQLDEQHHLTLMCRDLSEISHYARVDNSKFRLLKANTPGSYTFILE
                     ATKEVPRRLQHPKRSTIGIRVPDHPVALALLEELGEPISSSTLILPDEVWPLNDAELI
                     RELLEKKVDAVIDGGGVGTEFTTVIDLTGDAPVLLRKGKGDIAPFGIEE"
     gene            complement(3730..4611)
                     /locus_tag="VFW59_10280"
     CDS             complement(3730..4611)
                     /locus_tag="VFW59_10280"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HEX5364643.1"
                     /translation="MLDYDLHCHSNVSDGTLTPTEVVGRAAERGVKVLALTDHDDVAG
                     LDEARAAATRHGMGFVNGVEISVTWRSHTLHVVGLGIDPAYPPLAEGLRGVRSGRGER
                     AQKMADSLAKVGIGGVLQGAYQYAGNPNMIGRTHFARYLVESGQCKDVKSVFNRYLVK
                     GKPGYVSHQWADLADAIAWIRGSGGVAVLAHPGRYMVGRNSMGKKTMHELLVEFVELG
                     GQALEVVTGSHTPEQYAEFARYAEEFKLLCSCGSDFHGPGESWRDMGRLPDFPIGCRP
                     VWTVLPGLDPAPLAPTH"
     gene            complement(4611..4922)
                     /locus_tag="VFW59_10285"
     CDS             complement(4611..4922)
                     /locus_tag="VFW59_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX5364644.1"
                     /translation="MSDKINIEVVYALAHEQTLLKQQLPEGTTVGDAVRACGILDKHP
                     EIDLATTKLGIFGKLTKPDTVLRDKDRVEIYRPLIADPKEVRRKRAEEGKVMKKGGGD
                     I"
     gene            complement(4919..5356)
                     /locus_tag="VFW59_10290"
     CDS             complement(4919..5356)
                     /locus_tag="VFW59_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX5364645.1"
                     /translation="MALVEKTVLVPYSAEQMFNLVDRVEEYPQFLPWCGGASVAELDG
                     ATVHATVHIDYHHIKQHFTTENVRTPPHRIDMTLQDGPFKHLDGSWRFLPLSDSACKI
                     EFRLHYEFSSKLLEKLVGPVFHFIANSFVDAFIHRAEKVYTNV"
     gene            5419..5871
                     /gene="smpB"
                     /locus_tag="VFW59_10295"
                     /pseudo
     CDS             5419..5871
                     /gene="smpB"
                     /locus_tag="VFW59_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
     gene            6123..6965
                     /locus_tag="VFW59_10300"
     CDS             6123..6965
                     /locus_tag="VFW59_10300"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX5364646.1"
                     /translation="MARIPAEKPYLDVKDLQIDEWLELVFTPEKKRKFRFIDYEFPTD
                     NLCHDYIASLSVRSDADIKNLLRCFLIPTGGLGHDSTILHLWCESDQLLKQIDTTEYG
                     RRLIRGQAWEGITWILDLLPPWPQLAANALDAYIRAHAQVLPDGRFRGLCDVLTVIRA
                     KYLSAIHPKEVMECLSPREFEFLIAALFRAKGYKVRVTSQSRDGGADVLCWKHEGMVV
                     EKHLIECKHHTGKIGVPVVRQLAGVVSETGASKGVVVSSSSFSSPAIVFANKTGREGV
                     RNFV"
BASE COUNT         1462 a         2171 c         2011 g         1437 t
ORIGIN      
        1 tcccaaatat ttcgctttgc tggatttcca gcaggtattc cttggcaagt tcgaggatgg
       61 cgatgaaggt caccaccagc ttcggcgcgc cctcctccag atcgaacagt tgctcgaacg
      121 ggatgtattc gccgcctttc aggcagcgca gcacatgcgt catctgctcg cgcaccgaca
      181 gttgctcgcg gcgcaccttg tgatgcttgt tgagcttggc gcgcgccagc acgcccagcc
      241 atgcttggcg caggtcttcc acgcagattt ccggcagacg ctccttgacg gtcgtctcca
      301 tcagcacatg cacgatctcg aaatcgcgcc cggcctgcgg caattcgttg agctgctgcg
      361 cggcgagttt catctgctcg tattccagca ggcggcgcat cagttcgacg cgcgggtctt
      421 cgccgtcttc gtcgctgacc ttgggcgggc gcggcagcag catgcgcgac ttgatctcga
      481 tcagcaccgc cgccatcagc aggtattccg ccgccagttc cagcctgtgc tgctgcatca
      541 tctcgatgta gcccatgtac tgccgggtca gttccgccat cgggatgtcc agcacgtcga
      601 ggttgtgctt gcggatcagg tacagcaaca gatccagcgg tccctgaaaa gtctccagca
      661 ccagctccag cgcatccggc ggaatgtaca gatcctgcgg caaggccatc atcggctcgc
      721 cgtggatatg cgcaaacggg atcagctgga gctgctgttc gctcatcgtt cgaactcgga
      781 acgcggcaca ccggactgac gaatgatgga aagcaatgtg ccgacgcgaa tctcgtcgtg
      841 atccggaacc ggaacggtga tggtggaggt ttcagttttc aactgcatga cgatatgact
      901 gccgcgctgc ctcactttca ggaagccgtg cagcatgaga atggcgcaca attccctgcc
      961 ggaaagcacg gcaagtttag gcaaccgcga cctccagacg agtgacgtac acctcgttgt
     1021 gcaaacgtcc gtgtatttct tccggcgcgg cgcactccag aaacaattcg accgcctcgc
     1081 gaagattgct gcgcgcctga tcgacggtat ctccctgact ggcgacatcc agctcggggc
     1141 aaagcgaaac atagccatcg ccctcgcgct cgatcactgc ggtaaattgg taattcatat
     1201 cggcacctcc gaataacgtc cggtcaagta tagctcaggc ccatcgcctc gcgcacgtcg
     1261 cgcatggtct cggaagccag cttgcgcgcc ttctcgcagc cgtcggcgat gatgttgcgc
     1321 accagggcgg gatcgtccag atacagttgc gcgcgttcgc gcatcgggcg ctgttcttcc
     1381 agcaccgctt cgatcaccgg ctgcttgcat tcgatgcagc cgatgcccgc agtggtgcag
     1441 cccttctgca cccattgctt gacgtcgtcg ttcgagtaca cctgatgcag cgcccacacc
     1501 gggcatttct ccggcgtacc gcagtcggtg cggcggatac gcgccgggtc ggtcggcatg
     1561 gtgcggatct ttttcgtcac gctggcctca tcctcgcgca gcgtgatggt gttgttgtag
     1621 gacttggaca tcttctgccc gtccagcccc ggcatcttcg acgcggcggt gagcatcgcc
     1681 tgcggctcgg acaggatcat cttgccgctg ccttcgagat agccgaacag gcgctcgcgg
     1741 tcgccgtggc tgaggctctg ctgctcgtcc agcaaggctt tgccggactc cagcgcctcg
     1801 tcgtcgccct gctgctggta acgggtgcgc agttcggcat acagcttggc cttcttgctg
     1861 ccgagcttct tgaccgcagc ttcggccttt tcctcgaagc ccggttcgcg cccgtagatg
     1921 tggttgaagc ggcgcgcaag ttcgcgggtg aattcgatat gcggcacttg gtcttcgccc
     1981 accggcacct gcgtggcgcg gtagatcagc acgtccgcgc tcatcagcag cggatagccg
     2041 aggaagccgt aggtggacag atccttgctg gtcagttttt cctgctggtc cttgtaggtc
     2101 ggcatgcgct ccagccagcc cagcggcgtg atcatcgaca gcagcaggtg cagctcggca
     2161 tgctcgggca cgcgcgactg gatgaacagc gtcgcgtgcg cgggatcgac accggcggcc
     2221 agccaatcca ccaccatgtc ccacacgctc tgctcgatga cttgcggcgt gtcgtaatga
     2281 gtggtcagcg cgtgccagtc ggccacgaag aacaggcatt cgaattcgtg ctgcatccgc
     2341 acccagtttt tcaacacccc gtggtaatgg cccagatgca aatttccggt gggacgcata
     2401 cccgataaca cacgatcagc aaacatgatt tcctcaaagt ccaaataaca acgaaagcaa
     2461 ctggtacacc gccccgacca gcggcgagag tatccacccc aacaccggcg tgatcgccag
     2521 gaagatcagg atgaacatgc cgtaaggctc gatgcgggat aactggtagg cctgacgata
     2581 cggcaacagg ctgaccgcga cccggccgcc gtccagcggc ggcaacggca gcaggttcag
     2641 caccatcagc acggcgttga ttttgatgcc gatgcccgcc atttccagca gcggttcggc
     2701 gaaatagttg tcgggagtca ccaacgccag cttgtagagc agcgcccaac ccagcgccat
     2761 caccagattc gacgcgggcc cggcgagcgc gacccacatc atgtcctgct tggggcggcg
     2821 cagcgcgccg aagttgaccg gcacgggctt ggcccagccg aacaggatgc cgcccaacgc
     2881 cagcgtcagc agcggcaaca aaatcgtgcc aaccgggtcg atgtggcgcg ccgggttcag
     2941 gctgatgcgc ccctgcgcat aggccgtcat gtcgccgaag tggcgcgcca cgtagccgtg
     3001 cgcggcctcg tgcaaggtga tcgcaaacag gatgggaatc gcgccgatgg cgacggtttg
     3061 tatcagttgt tcgacttgca ttattcctcg attccgaatg gcgcgatgtc gcccttgcct
     3121 ttgcgcaaca acaccggcgc gtcgccggtc aggtcgatca cggtggtgaa ctcggtccct
     3181 accccgccgc cgtcgatcac cgcatcgact tttttctcca gcagctcgcg gatcagttcg
     3241 gcatcgttca gcggccagac ttcgtcgggc aggatcaaag ttgagctgga aatcggttcg
     3301 cccaactcct ccagcaacgc cagcgccacc ggatgatccg gaacgcggat gccgatggtg
     3361 ctgcgcttgg gatgctgcaa gcggcgcggc acttccttgg tcgcctccag gatgaaggtg
     3421 tagctgcccg gcgtgttggc cttcagcagc cggaacttgc tgttgtcgac gcgcgcgtaa
     3481 tgcgaaatct ccgacaggtc gcggcacatc agcgtgaggt gatgctgctc gtccagttgg
     3541 cggatctggc ggatgcgcgc aaccgcctcc ttgtcgtcca ggtggcaacc cagcgcatag
     3601 caggagtcgg tcgggtacac cacgatgccg ccgtcgcgca gcaccgccgc cgcctgacgg
     3661 atcaaccgcg cattgggatt gtccagatga atattgaaaa attgagccat cgttgtttat
     3721 cgcagaaatt taatgggtcg gagccaatgg cgcgggatca aggccgggca acaccgtcca
     3781 gaccggacgg cagcctatcg ggaagtccgg cagccgcccc atgtcgcgcc agctttcgcc
     3841 cgggccgtgg aagtcggaac cgcacgaaca cagcagcttg aactcttctg cataacgcgc
     3901 aaactcggca tactgttccg gcgtatggct gccggtcacc acttcgagcg cctgcccgcc
     3961 cagctcgacg aactccacca gcaactcgtg catggtcttc ttgcccatgc tgttgcgtcc
     4021 gaccatgtag cggcccggat gcgccagcac cgccacgccg ccgctgccgc gtatccaggc
     4081 gatcgcatcg gccaaatccg cccactggtg cgacacgtag ccgggcttgc ccttcaccag
     4141 ataacggttg aacacgctct tcacgtcctt gcactgcccg gactcgacca gatagcgcgc
     4201 aaagtgcgtg cgcccgatca tgttggggtt gcccgcatac tggtacgcgc cttgcagcac
     4261 accgccgatg ccgaccttgg ccagcgagtc ggccattttt tgagcccgct cgccgcgtcc
     4321 gctgcgcacc ccgcgcaagc cttccgccag cggcggatag gccgggtcga tgcccagccc
     4381 gacgacgtgc agcgtgtggc tgcgccaggt cacggaaatc tccacgccgt tgacgaagcc
     4441 catgccgtgc cgcgttgcgg cggcgcgcgc ctcatccagc ccggccacgt cgtcgtgatc
     4501 ggtcagcgcc agcaccttca cgccgcgctc cgccgcgcgc cccaccactt cggtcggggt
     4561 cagcgtgccg tcggagacgt tggaatggca atgcaggtcg taatcaagca tcagatgtcg
     4621 ccgccgccct ttttcatgac cttgccctct tcggcacgct tgcggcgcac ttccttgggg
     4681 tcggcgatca gcgggcggta gatttcgacg cgatccttgt cgcgcagcac cgtatcgggc
     4741 tttgtcagct tgccgaaaat gcccagcttg gtggtggcca agtcgatctc gggatgtttg
     4801 tcgagaatgc cgcatgccct gacggcgtct cccaccgtcg tcccttccgg caactgctgc
     4861 ttgagcagcg tttgctcgtg ggccagtgcg tacaccactt cgatattgat cttgtcgctc
     4921 atacgttcgt ataaaccttc tctgcgcggt ggatgaaagc atccacgaaa ctgtttgcaa
     4981 tgaaatgaaa aaccggcccg accagcttct ccagcagctt gctggaaaac tcgtagtgca
     5041 ggcgaaactc tattttacag gctgagtcgc tcaacggcag gaatcgccaa ctgccgtcca
     5101 gatgcttgaa cggcccgtcc tgcaacgtca tgtctatccg gtgcggcggc gtgcgcacgt
     5161 tctcggtggt gaaatgctgc ttgatgtggt ggtaatcgat atgcaccgtg gcatgcaccg
     5221 tcgcgccatc cagctcggcg acgctcgcgc cgccgcacca tggcagaaac tgcggatatt
     5281 cctccacgcg atccaccaga ttgaacatct gctccgcgct atacggaacg agtaccgttt
     5341 tctcgaccag tgccattgtg actaaagcaa taaaaccaaa gactgctaga atagccgaac
     5401 acaccgcaaa actcacctga tatgagcatc atccagaaca aaaaagcctt tcacgaatac
     5461 ttcatcgagg acaaatacga agccggcatc atgctggaag gctgggaagt caaagccatc
     5521 cgcgccgggc gcgcgcaact caaggaagcc tacgtgaccg tcaaggacgg cgcgctgtac
     5581 ctgttcggct cgcacatcag ccccctgctg aacgcctcca cgcacatcca ccccgacccg
     5641 acccgcgtcc gcaaactgct gctgcacaaa cacgagatcg acaagatcat cggcaaggtg
     5701 caacgcgcgg gctacacgct gatgccgctg gacatgcact acaagggcgg gcgcgtcaaa
     5761 ctggagatcg gcctggcgaa gggcaagaaa cagcatgata agagggccac cgaacgcgaa
     5821 aaggatgcaa agcgcgaagc cgcccaagcg atgaaaaagg aacgccgcta gttgcgtggc
     5881 ccttttacgg cgcaggctaa cctgtgcttg cacaggttaa gctcgcgaag cgagcggccg
     5941 tagccacaaa agagccactg ccccatgcgc accgctaagc cctatgccgt gttatgtttt
     6001 gccttgccaa agcaatcaca ggaatgcaaa ccatgaagca gcttatacgg cagttcgttc
     6061 cagaatatcc ggcgtttttt gccatctacg ttacgttagc acccttatag accattgcat
     6121 tcatggcacg aattcccgca gaaaagccat acctcgatgt caaagacctc caaattgatg
     6181 agtggctcga actcgttttt acacctgaga agaagagaaa attcagattc attgattacg
     6241 agtttccgac ggataaccta tgtcatgact acatcgcctc gttatcggtt agaagtgatg
     6301 ccgatataaa gaatttactg cgatgcttct taattccgac gggcggcctc ggacacgact
     6361 caacaattct ccacctttgg tgtgaaagcg atcaattact caagcaaatt gataccacgg
     6421 aatatggacg aaggttaatt cgaggtcagg cttgggaagg aattacatgg atcttggact
     6481 tattgccgcc ttggccacaa ttggccgcaa acgctcttga tgcatatatt cgcgctcacg
     6541 cacaagtcct accagatggt cgttttcgtg gcctttgcga tgtactgaca gtcattcgtg
     6601 ccaagtatct ttctgcgata caccccaagg aagtaatgga gtgtttgtca ccacgtgagt
     6661 ttgaatttct gattgcagca ctgtttcggg ccaagggata caaagtcagg gtaacttcgc
     6721 aaagtcgaga tggcggtgca gatgtcctct gctggaaaca tgagggcatg gttgtcgaaa
     6781 agcaccttat tgagtgcaaa catcacactg gaaaaattgg agtgcccgta gttcgccaat
     6841 tggccggggt tgtttctgaa acgggagcca gtaaaggtgt agtggtgtca tcaagtagtt
     6901 tctctagtcc ggcaattgtt tttgcaaata aaaccggtcg cgagggtgta agaaattttg
     6961 tgtaaacggt cataaggcag gaaactgccg ccactttgaa aggagtcgca atgaccgtag
     7021 caaaagcact acccgcagag ctgatcgaca gcctgctgtc tggatataaa aaaccggaag
     7081 a
//
LOCUS       DASUIV010000288        14291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_75531, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000288 DASUIV010000000
VERSION     DASUIV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 14291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14291
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75531"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFW59_10305"
     CDS             complement(<1..708)
                     /locus_tag="VFW59_10305"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX5364647.1"
                     /translation="MGHPLWQVGFRPFFALAMLAGLSLPVLWALFFSGVVSAPQTSFS
                     MVQWHAHEMFFGFGWAVLGGFLLTSTKNWVKVRGYHGLALMFLAAAWLFERIGMWFEG
                     ALFDGFWAHALFLLSNNLFLGSIVAMLAFTLVKNRATDSYRNNYLFLLILPAFLLAKY
                     LMLSTDHYLLGWSMALALVRMAFLVMLERTLVQFMKGAFQVGILQHPLLDGAIKLLGL
                     LLVLGGLLPSVLTACAGL"
     gene            complement(719..979)
                     /locus_tag="VFW59_10310"
     CDS             complement(719..979)
                     /locus_tag="VFW59_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX5364648.1"
                     /translation="MTHNCANSFSQDMRYPFDARGVAKRFRHAAIFGALDSLNPGETM
                     SFVNDHDPLPLLAQLQQRYGAAIEISYVAREPGNIAIDFTRR"
     gene            complement(1008..1481)
                     /locus_tag="VFW59_10315"
     CDS             complement(1008..1481)
                     /locus_tag="VFW59_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5364649.1"
                     /translation="MKLTHYSDLGLRLMMYLSLRYGEAVTIGEASERLAVSKNHLVKV
                     SHQLTRAGLIESLRGRNGGVRLAFAPDAITVEQVLRATEDNFDLVECQAGGEIRCIIA
                     GPCKLSAALEDAREAFFASLRRVSMADLVKNGKGLERALQPLSSVARIPVRSATK"
     gene            complement(1516..2436)
                     /locus_tag="VFW59_10320"
     CDS             complement(1516..2436)
                     /locus_tag="VFW59_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEX5364650.1"
                     /translation="MASERIRKLLGVEPNNTGHGEKLISALGALVAIAATMAVSLHVV
                     GVQGAALLVASMGASAVLLFAVPHGALSQPWPLVGGNLVSALIGVSCAQLIHDPFVAG
                     PLAVALAIFAMHYLRCIHPPGGATALVAVVGGPAVQALGYQYLLTPVLLNVGIMLLAA
                     VAVNSLFPWRRYPASLRRVTVAAPQAVPQEQLGHADFAYALREIGSYLDVTESDLAKI
                     YRLARKHALRTSHESEPLAAGNYYSNGEYGEQMTVRKVSGLEGDAVSYRVVAGFGESD
                     TATATRAEFMAWQRYEVIPAEGAWHRIMRL"
     gene            2519..2902
                     /locus_tag="VFW59_10325"
     CDS             2519..2902
                     /locus_tag="VFW59_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEX5364651.1"
                     /translation="MDTVSYTLLKHLHMTCAAASYSLFLLRGIWMLRDSPVMQQRWVK
                     IAPHLIDTALLASAFALAYTIGQYPFVDSWLTAKVLALLLYIALGLVALKYGRSKPVR
                     LAAWLAAQIVFAYIVLVAVNHTPFI"
     gene            2914..3471
                     /locus_tag="VFW59_10330"
     CDS             2914..3471
                     /locus_tag="VFW59_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5364652.1"
                     /translation="MISLDSAASAPTFDHPLEMLHACHGKILRQCDTLQKLAAHLAQH
                     GCDTQARQAAQGILRYFDTAGQFHHQDEEENLFPSLRAGDSAPQLDALLQRLLGEHVV
                     MLAAWAELRDMLRQLAEGANVPLSDALLARFIRSYREHIALEEAELLPLAARLLNAAQ
                     TRQIGMSMAARRGASFPAPHQPPTS"
     gene            complement(3461..5806)
                     /locus_tag="VFW59_10335"
     CDS             complement(3461..5806)
                     /locus_tag="VFW59_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEX5364653.1"
                     /translation="MLPSIEQRLAVEIVAKPVQIAAAIALLDEGATVPFIARYRKEAT
                     GGLDDIQLRMLEDRLRYLRELEARRAAIIASITEQGKMTPELLDAVAHAEDKTYLEDL
                     YLPYKPKRRTKAQIALEAGLEPLADGLLHDPMLNPEEEAAKYLKEAFTNELGDNNPGV
                     PDAKAALDGARQILMERFAEDAALLQSLREYVQEHGVVESKVVEGKEEAAAKYADYFD
                     YMESINTIPSHRALAVFRGRREELLNVQLRLDSEEEKPAWNAPLNPCELRIAKRCGIS
                     QQNRPADKWLADTVRWTWRIKSFMHLESELMGTLRANAETEAINVFARNLKDLLLAAP
                     AGPRATMGLDPGVRTGVKVAVVDETGKVVDTAVIYPHQPRNDWEGSLHTLAKLAEKHK
                     VALIAIGNGTASRETDKLAGDLIKRYPEAKLTSIVVSEAGASVYSASEFASRELPDMD
                     VALRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHDVSQTQLARSLDAVVEDCVNAVG
                     VDVNTASAPLLARVSGLSAGVAQAIVTYRDLKGRFTSRAQLREVPRLGDKTFEQAAGF
                     LRIMGAADPLDASAVHPESYPLVQRILADIKQDIKAVIGNGALLKSLNPAKYMDEQFG
                     IPTITDILKELDKPGRDPRPEFTTATFREGVEELSDLQPDMILEGVVTNVAAFGAFVD
                     IGVHQDGLVHISALSNTFVKDPHSVVKAGQVVKVKVLEVDVKRKRIALTMRLTDSAPQ
                     GGAKPAPRDARAGGKMPERQRSAPQPELQGAMAAALARLKK"
     gene            5980..6387
                     /locus_tag="VFW59_10340"
     CDS             5980..6387
                     /locus_tag="VFW59_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364654.1"
                     /translation="MMEYIFFNADLRDRFVEHARSLGVASELHDDAMGLVVALEEDLG
                     EEREDSIEACYEKLEEEQARLLAQEEGGLKQIAGFRVNLPDGESGLVPLPPELANRLM
                     ASFSLEEIQTLLDTVARSVLEPRQHLCEILRSK"
     gene            complement(6415..7710)
                     /locus_tag="VFW59_10345"
     CDS             complement(6415..7710)
                     /locus_tag="VFW59_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5364655.1"
                     /translation="MTTQESVLPASDAATLLFIDDEANILAALKRLFNPLGYRILTAE
                     SGVEALMLLEKERVDLVISDMRMPQMSGAEVLEQIRIKWPDIVRLLLTGYSDVNSTIA
                     AVNRGEIYRYIAKPWDDNDIVLMVRDALERKHLLDEKLRLEARVLRQNEELIQLNASL
                     EDRVRQRTEDLNIVLELLKDDYFTTVQVFANLMELRKGAMAGHSRRVAQLCRDLAHKM
                     LLSQADTNNLVTAALLHNIGKIGLPNHLLETPYMELSSAERAEFDKHPRYAAATLMAL
                     NPLKMTAKLIRYHREHYDGVDNASGLRGEGLPLGSRILLVASDFDALQQGLITHGKLS
                     PDQAIDRIVSGRNTIYDPIVVDVFRQLMPQIGGDVGAEFLVTSAQLHEGMRLTRDVVT
                     ANGMLLLFKDTVLNAECILDIREIEQSEGVQLPLYTDVI"
     gene            complement(8104..8358)
                     /locus_tag="VFW59_10350"
     CDS             complement(8104..8358)
                     /locus_tag="VFW59_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX5364656.1"
                     /translation="MEEILQYLKKYGDRLDKEISVATGISLATVRVHLSELAAKGEIM
                     VCRSIRFEKGQEIEGVRCRLAGYIPPAAPGRKPKTQLKLS"
     gene            8582..9004
                     /locus_tag="VFW59_10355"
     CDS             8582..9004
                     /locus_tag="VFW59_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HEX5364657.1"
                     /translation="MLRKFIVSNLAMAAITVLMAQPVLAETPDEMLTLIKKEARTGDA
                     SFQGFSASRGEKFFKQKQGGEWSCSSCHGDNPAATGKHAKTDKTIQPMAPAANAERFT
                     DPKKVAKWFKRNCNDVLNRVCTPQEKGDVLSYLLTVKK"
     gene            9015..9506
                     /locus_tag="VFW59_10360"
     CDS             9015..9506
                     /locus_tag="VFW59_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX5364658.1"
                     /translation="MKSTCTNIARRLAWALLITGAAITSAHAKHDENKPLTAAGNGQW
                     QAECGSCHIPYSPRLLHAESWRAVMSGLDRHFGTDASLDAATTQQIGSFLERNAGSRK
                     EPASAKPVLRISETRWFIREHRKLSSKVWTSDLVKSPANCTACHASEPSGNYSERNVS
                     VPQ"
     gene            9519..10187
                     /locus_tag="VFW59_10365"
     CDS             9519..10187
                     /locus_tag="VFW59_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5364659.1"
                     /translation="MNQRMKVWDLPTRVFHWLLALSFSGAFLTAESDRYRDIHLVLGY
                     TLLGLIAFRLVWGFVGTRYARFRSFLFGPGETKAYVTSLFKSQPKHYAGHNPAGSLAV
                     FALLGLGIASGLSGVLLLQDAGGEALEELHELVSYGMLAVVVIHVIGVLFSSMMHREN
                     LVRAMITGFKPAQPNEGIRRTHAWLGVIILAAVLAFWWGYPATGLVTPNSDVAQSQND
                     DDDD"
     gene            10216..10881
                     /locus_tag="VFW59_10370"
     CDS             10216..10881
                     /locus_tag="VFW59_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5364660.1"
                     /translation="MRILVIEDDVLLGDAIQAGLKQAGYAVDWMKDGITADQALSTEP
                     YAAAVLDLGLPRMAGLEVLRRLRSRNIPIPVLILTAMDTVEDRIKGLDAGADDYLAKP
                     FDMGELAARLRALLRRASGKTAPALQAAGVELNPATHAVSYRGQVIDLPAKEFAVLHA
                     LMLNTGRVLSRGQIEEQLYSWGEEVESNAVEVYIHHLRRKLFPGLIETIRGVGYLIPA
                     ANI"
     gene            10883..12214
                     /locus_tag="VFW59_10375"
     CDS             10883..12214
                     /locus_tag="VFW59_10375"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364661.1"
                     /translation="MRKHSSLKQRLLALALTTVILVWAGAITFTYFDAREEFDEVLDA
                     YLAQSASLLVIQASRDLDEIKTDHAPLLHKYSRHVAFQIWENGQVLRLHSANAPASPL
                     SVREHGFSDTVIDGESWRVFSIWDDGKKNLIQVAERTEVRDELAGGIAGNLLTPLLVS
                     LPLLALLLWVSVTRGLHPLFRLTAEVAQREPDNLAPLEIASAPREVAPLIERLNSLFV
                     RIDVSLQKERRFTADAAHELRTPVAGIKAQAQVARAASSDKERIHALDNAILGCDRAA
                     HLIDQLLTLARIDTLDEEITEACQLRALAAEVIAAIAPTALAQGVQIELSEGDEVRVP
                     GSSTLLRILLRNLIENAVRHTPTGTLVQVGITLQQGHPCLSVCDNGPGIAKADLDRIA
                     ERFYRPVGTSASGSGLGLSIVKRIAEIHAAELKISPRENGKGLDISVIFNA"
     gene            12613..12933
                     /locus_tag="VFW59_10380"
     CDS             12613..12933
                     /locus_tag="VFW59_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5364662.1"
                     /translation="MKSIIVSLAATGLLIAGNAMAIEMPTDGKTKCGACHKIDTKAVG
                     PAWKDVAAKYKGKADAEKTLIANITKGGQFGWKQGNMPAKGMGANDAQIAALAKFIAV
                     DLNK"
     gene            complement(13029..13655)
                     /locus_tag="VFW59_10385"
     CDS             complement(13029..13655)
                     /locus_tag="VFW59_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5364663.1"
                     /translation="MRSLIQHFAAGVVLAALAVELLPEIGREHAPGLVLVGSFALGSL
                     FMYGLKLWTMRLEHQVSVSGAAAGLGTGLLLATFIDVATDGFIIGAGFAAGGETGTIL
                     ALGLSVELLFLGLALASEATAGWRIIAISGALGGTVLTFAVIGSALLAGASHAVIGGA
                     LAFSAAALLYLVTEELLMEAHEVEEKPISTLVLFGGFLAFWSIQLLGQ"
     gene            complement(13853..14101)
                     /locus_tag="VFW59_10390"
     CDS             complement(13853..14101)
                     /locus_tag="VFW59_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX5364664.1"
                     /translation="MTERAGIEIDYCPQCRGVWLDRGELDKIIERSMSELAQIAPVMP
                     SSRPVQQPYDNNHGYHGKHHDGHHGYRGKKSLWQELFD"
     gene            complement(14094..>14291)
                     /locus_tag="VFW59_10395"
     CDS             complement(14094..>14291)
                     /locus_tag="VFW59_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364665.1"
                     /translation="RLRRKTDRKAAKKRTSQPSVARWMIRFKQIFFKPSPDLRRFRCG
                     HLFKKDQHEMSRLPQHQSAYD"
BASE COUNT         3013 a         4469 c         4089 g         2720 t
ORIGIN      
        1 cagtccggca caggccgtca ggacggacgg caacagcccg cccaacacca gcagcagacc
       61 gagcaatttg atcgccccat cgagcaaggg atgctgcagg atgccgacct ggaacgcacc
      121 cttcatgaac tgcaccagcg tgcgctccag catcaccaga aaggccatac ggaccagtgc
      181 cagcgccatg ctccagccga gcaggtaatg atccgtgctc agcatcagat acttggccag
      241 caagaaggcc ggcaggatca gcaggaacag atagttattg cgataggagt cggtcgcgcg
      301 attcttcacc agcgtgaagg ccagcatcgc gacgatggaa ccgaggaaca ggttgttgga
      361 cagcaggaac agcgcatgcg cccagaagcc atcgaacaag gcgccctcga accacatgcc
      421 gatgcgctcg aacagccagg ccgccgcgag gaacatcagc gccagcccgt gatagccgcg
      481 caccttcacc cagttcttcg tggacgtcag cagaaaaccg cccagcaccg cccaaccgaa
      541 gccgaagaac atctcgtggg cgtgccactg caccatgctg aaactcgtct gcggtgccga
      601 aacgacaccg gagaagaaca gtgcccacag caccggcagg ctcagcccgg ccagcatcgc
      661 cagcgcgaag aatggccgga agcccacctg ccagagcgga tgacccatgc tgcgctgcct
      721 agcggcgcgt gaagtcgatg gcgatattgc ccggctcgcg cgcgacatag ctgatctcga
      781 tcgcggcacc gtagcgctgc tgcagctggg ccagcagcgg caacggatca tggtcgttga
      841 cgaaggacat ggtctcgccc ggattcagcg aatcgagtgc accgaagatg gcggcgtgac
      901 ggaagcgctt ggcaacaccg cgcgcgtcga acgggtagcg catgtcctgg gagaagctgt
      961 tggcacagtt gtgggtcatg gtgtgttcct tggttggttg taaaaaatca tttcgtcgcg
     1021 cttctgaccg gtatccgggc gacgctgctc aagggttgca gcgcgcgttc cagccccttc
     1081 ccgttcttga ccaggtcggc catgctgacc cggcgcaagc tggcaaagaa agcctcgcgc
     1141 gcatcctcca gcgccgcgct caacttgcac gggccagcga tgatgcaacg gatctcgcct
     1201 ccggcctgac actccaccag atcgaagtta tcctcggtcg cgcgcaacac ctgttcgacc
     1261 gtgatcgcat ccggcgcaaa ggccagacgc acgccgccgt tgcgtccgcg cagactctcg
     1321 atcaggccgg cacgcgtcag ttggtgcgag accttgacca gatgattctt ggacaccgcc
     1381 agccgctcgc tcgcctcgcc tatcgtgacc gcctcgccgt agcgcaacga cagatacatc
     1441 atcaggcgca atccgagatc ggaataatgg gtcagtttca ttcctgacct tcgagcatgg
     1501 gggattcgct atgcactaca gcctcatgat gcgatgccat gcgccctccg ccgggatcac
     1561 ttcataacgc tgccaggcca tgaattcggc ccgtgtcgcc gtggcggtat cactctcgcc
     1621 aaacccggca acgacgcggt aactcacggc gtcgccctcc agcccggaga ccttgcgcac
     1681 cgtcatctgc tcgccatact cgccgttgct gtagtaattg cctgcggcaa gcggctcgct
     1741 ctcatgcgag gtacgcaacg catgcttgcg tgccagccgg taaatcttcg ccagatcgct
     1801 ctcggtgaca tccagatagg aaccgatctc gcgcagcgca taggcgaaat cggcatgccc
     1861 cagttgctcc tgcggtaccg cctgcggcgc cgcaactgtc actcttctga ggctggccgg
     1921 atagcggcgc cagggaaaga gcgagttcac cgcaacggcc gcaagcaaca tgatgccgac
     1981 gttcagcagc accggggtca gcaggtactg gtaaccgagt gcctgcaccg ccggcccgcc
     2041 gacgacggcg accagcgcgg tcgcaccgcc gggcggatga atgcagcgca ggtagtgcat
     2101 cgcgaagatg gcgagtgcga cggccagcgg cccggccaca aagggatcgt ggatgagctg
     2161 cgcgcaactc accccgatca gcgccgacac cagattgccg ccgaccagcg gccagggttg
     2221 cgacagcgca ccgtgcggca cggcgaacag caacacggcc gaggctccca tcgaagccac
     2281 cagcaacgcc gcgccctgca cacccacgac atgcagactg accgccatcg tcgccgcaat
     2341 cgcaaccagc gcacccagtg ccgaaatgag cttctcgcca tgcccggtat tgttcggctc
     2401 cactcccagc agttttcgta tccgttcaga cgccatatcg cgaactccag caccggttta
     2461 atgatgcgcg aatgatacat gttttatgtt atcttggcga aactgcaaac gaaccggaat
     2521 ggataccgtg agttacaccc tgctcaaaca cctgcacatg acctgcgcgg ccgccagtta
     2581 ctcgctgttc ctgctgcgcg gcatctggat gctgcgcgac tcgcccgtca tgcaacagcg
     2641 ctgggtgaag atcgctccgc acctcatcga cacggcgctg ctcgccagcg ccttcgcgct
     2701 ggcttacacc atcggacaat atcctttcgt ggatagctgg ctgaccgcca aggtcctcgc
     2761 actgctgctg tatatcgcac tggggctggt cgcgctgaaa tacggccgca gcaaacccgt
     2821 acgccttgct gcctggctgg cagcgcagat cgtgttcgct tacatcgtgc tggtcgcggt
     2881 gaaccacaca ccgtttatct gacaggaacg aaaatgatct ctctggactc cgctgcaagc
     2941 gccccgacct tcgaccatcc gctggagatg ctgcatgcct gccacggcaa gattctgcgc
     3001 cagtgcgaca cgctgcaaaa actggccgcc catctggcgc aacatggctg cgacacccag
     3061 gcccgacaag ccgcacaggg catcctgcgc tacttcgaca cggccggaca gttccatcat
     3121 caggacgagg aggagaacct gttcccctca ttgcgcgccg gcgacagcgc accgcaactg
     3181 gatgcgctgc tgcaacggct gttgggggaa catgtcgtga tgctggccgc gtgggccgaa
     3241 ttgcgcgaca tgctgcggca actcgcggaa ggcgcgaacg tgccgctgag cgatgcgcta
     3301 ttggcaagat tcatccgcag ttaccgcgaa cacatcgcgc tcgaagaagc ggaactgctg
     3361 ccgcttgccg cgcgcctgct gaacgccgca cagacccggc agatcggcat gagcatggcc
     3421 gcgcggcgcg gcgcgagttt ccccgcaccg caccagcccc ctacttcttg agccgggcca
     3481 gcgccgccgc catcgcgccc tgaagctcgg gttgcggtgc agaacgctgg cgctcgggca
     3541 tcttgccgcc agcacgcgcg tcgcgcggtg ccggtttggc cccaccctgc ggcgcactgt
     3601 cggtcaggcg catggtgagc gcgatgcgtt tgcgcttcac gtccacttcc agcaccttca
     3661 ccttcaccac ctgacccgcc ttgaccacgc tgtgcggatc cttgacgaag gtattcgaca
     3721 gcgccgagat atgcaccagc ccgtcctgat gcacgccgat atcgacgaat gcgccgaacg
     3781 ccgcgacgtt ggtgaccacg ccctcgagga tcatgtccgg ctgcagatcg gacaactctt
     3841 ccacgccttc gcggaaagtc gcggtggtga actccggacg cgggtcgcgc cccggcttgt
     3901 ccagctcttt gaggatgtcg gtaatggtcg gaataccgaa ctgctcgtcc atgtacttcg
     3961 ccgggttcag cgacttcagc agcgcgccgt taccgatcac cgccttgatg tcctgcttga
     4021 tgtcggcgag gatgcgctgc accagcggat aggactcggg atgcaccgcc gaggcgtcga
     4081 gcggatcggc cgcgcccatg atgcgcaaaa agcccgcagc ctgctcgaag gtcttgtcgc
     4141 ccagacgcgg cacttcgcgc agttgcgcgc gcgaggtgaa acgccctttc aggtcgcggt
     4201 aagtcacgat ggcctgcgcc acgccggcgg aaaggccgga gacccgcgcc agcagcggcg
     4261 cggatgcggt gttcacgtcc acgcccaccg cattcacgca gtcctccacc accgcatcca
     4321 gcgaacgcgc cagctgggtc tggctgacat cgtgctgata ctggccgacg ccgatggact
     4381 tggggtcgat cttcaccagc tcggccagcg ggtcctgcag gcggcgcgcg atcgagaccg
     4441 caccgcgcaa cgccacatcc atatcgggca gttcccgcga ggcgaactcg gaggcggaat
     4501 acaccgacgc gcccgcctcg gacaccacga tgctggtcag cttcgcttcc ggatagcgct
     4561 tgatcaagtc gcccgccagc ttgtcggtct cgcgcgaggc ggtgccattg ccgatcgcga
     4621 tcagcgcgac cttgtgtttc tcggccagct tcgccagcgt atgcagcgag ccttcccagt
     4681 cgttgcgcgg ctggtgcgga tagatcaccg ccgtgtccac caccttgccg gtctcgtcca
     4741 ccaccgccac cttgacgccg gtgcgcacgc cgggatcgag gcccatggtc gcacgcgggc
     4801 cggcgggagc ggccagcagc aggtctttca gattgcgcgc aaacacgttg atcgcctcgg
     4861 tctcggcatt ggcgcgtagc gtgcccatca gctcggattc cagatgcatg aaagacttga
     4921 tgcgccaggt ccagcgcacc gtgtcggcca gccacttatc ggccggacgg ttctgctgcg
     4981 agatgccgca gcgcttggcg atgcgcagtt cgcagggatt cagcggcgcg ttccaggccg
     5041 gcttctcctc ttcgctgtcc agccgcagct gcacgttcag caactcctcg cggcggccgc
     5101 ggaagaccgc cagcgcgcgg tgcgacggaa tggtgttgat ggactccata tagtcgaagt
     5161 agtcggcgta ctttgccgcc gcctcctcct tgccctcgac cactttcgat tcgacgacgc
     5221 cgtgttcctg cacgtattcg cgcagcgatt gcagcagcgc cgcgtcctcg gcgaagcgct
     5281 ccatcaatat ctggcgcgca ccgtccagcg ccgccttggc gtcaggcacg ccgggattgt
     5341 tgtcgcccag ttcgttagtg aacgcctcct tcaggtactt ggccgcctct tcctccgggt
     5401 tcagcatcgg gtcatgcagc agtccatcgg ccaaaggctc cagtccggct tccagtgcga
     5461 tctgcgcctt ggtgcggcgc ttcggcttgt agggcagata caggtcttcc agataggtct
     5521 tgtcctcggc atgggcgacg gcgtccagca gctccggcgt catcttgccc tgctcggtga
     5581 tcgaggcgat gatggccgcg cggcgcgctt ccagttcgcg cagatagcgc agccggtctt
     5641 ccagcatgcg cagctggatg tcgtccagcc cgccggtggc ctccttgcgg taacgggcga
     5701 taaagggaac cgttgcgccc tcgtccagca gcgcgatggc ggcggcgatc tggacaggct
     5761 tggcgacgat ctcgacggcg agtcgttgtt cgatggatgg cagcatggga gtattccggg
     5821 tctgtgcagg gcattccgga accagtgccc gggatgccgc gtgaattacg aagggcgcgc
     5881 attatgccgc atttaaaccg tcacgggcac cctccggaaa cgccgaactg aggcataatc
     5941 tgcccgcttt tccagaacac tcaccggagt acccgcacca tgatggaata catatttttc
     6001 aacgccgacc tgcgcgaccg gttcgtcgaa cacgcccgca gcctgggcgt tgccagcgaa
     6061 cttcacgacg acgccatggg gctggtggtc gcgctggaag aagacctggg cgaggaacgt
     6121 gaagacagca tcgaggcctg ctacgaaaaa ctggaagaag aacaggccag actgctcgcg
     6181 caggaagaag gcggactgaa acagatcgcg ggtttccgcg tcaacctgcc ggacggcgag
     6241 agcggcctgg taccgctgcc gccggaactg gccaaccgcc tgatggccag cttcagcctc
     6301 gaggagatac agaccctgct cgacaccgtc gcccgcagcg ttctcgaacc ccgccagcac
     6361 ctgtgcgaaa tcctgcgatc gaaataagcc cgaagacaag aacgcgctgc ctgcctagat
     6421 aacgtcggta tagaggggca gttgcacgcc ttcagactgc tcgatttctc gtatatcaag
     6481 gatacactcg gcattcagca ccgtatcctt gaacagcagc aacatgccgt tggctgtcac
     6541 cacatcgcgc gtaagccgca tcccttcatg caactgggcg ctggtaacca aaaactcggc
     6601 cccgacatcg ccgccgatct gcggcatcag ttgacggaac acatccacca cgatcggatc
     6661 gtagatcgta ttgcgtccgc tgacgatcct gtcgatcgcc tgatccggcg ataacttgcc
     6721 atgggtgatc agcccctgtt gcagggcatc aaagtcgctc gccaccagca ggatacggga
     6781 acccaacggc agaccttcgc cgcgcaggcc ggatgcattg tccactccat cgtaatgttc
     6841 gcgatggtag cggatcaact tggcagtcat cttaagcgga ttgagcgcca tcagggtcgc
     6901 tgccgcatat ctcggatgct tgtcgaattc ggcgcgctcg gcagatgaca attccatata
     6961 aggcgtttcc agcaggtgat tcggcaggcc gatcttgcca atgttatgca gcagcgccgc
     7021 cgtcacgagg ttattggtat cggcttgcga caggagcatc ttatgcgcga gatcgcggca
     7081 cagttgcgcg acccggcgcg aatgacctgc catcgcgcct ttgcgcagct ccatcagatt
     7141 cgcaaacacc tgaacggtgg tgaaataatc atccttcagc agctccagca cgatgttcag
     7201 atcttcggtg cgctggcgca ccctgtcctc caggctggca ttgagctgta tcaactcttc
     7261 gttctggcgc aagacccttg cttccagccg cagcttctcg tccagcaagt gtttgcgctc
     7321 cagcgcatca cgaaccatca gaacgatgtc gttgtcgtcc cacggcttgg caatgtaacg
     7381 atatatctcg ccgcgattga ctgccgcgat ggtcgaattc acatcggaat agcccgtcag
     7441 caggaggcgc acgatgtccg gccacttgat gcgtatctgt tccaatacct cggcaccgct
     7501 catttgcggc atgcgcatgt ccgagatcac cagatccact cgctctttct caagcagcat
     7561 gagcgcttcc acgccgcttt ctgccgtgag gatgcgataa cccagcgggt tgaacaaacg
     7621 ctttagcgcc gccagaatat tggcctcgtc gtcgatgaac aacagggtcg cagcatcact
     7681 tgcaggcagt accgattctt gagtcgtcat gtcatctcct tgatgagcgg attgttatac
     7741 cgatcgccag attcaaaaca ctatcgcgca gcccctgctg caccagacag ttcatccagc
     7801 acgcaggcat gccatttcgt tgctattgct aaggatggca taaccccgca actgcgagcc
     7861 ttgctccggg caagattact cgcaaatgcc attcattgga tatagggatt gatggcaagc
     7921 ttgcctagga aggcgatgat gacgattcgg ccatggcgtt gtttattggc tttgccaatt
     7981 gacggttcat ggcgcagggc cgcagctcac ttcgtgatgc tgggtggcgg cagcgcgcac
     8041 cggtgttcga tcatggccga gcctgcagcc aattgaaagg gccgtttatg acgcaccggc
     8101 caactaggat aatttcagct gtgtcttggg cttcctgccc ggtgccgcag gcgggatata
     8161 ccccgccagc cgacagcgaa ctccctcgat ctcttgccct ttttcaaatc tgatcgaacg
     8221 gcacaccatg atctcgcctt tcgccgccag ttccgacaaa tggacacgca ccgtagcgag
     8281 cgagatgcct gtggcgacgg aaatttcttt gtcgagccga tcgccatact tcttcaggta
     8341 ttgcagaatt tcttccatcg atttattacc atgaacagcg cggtgaatga tgggtcagta
     8401 tatacccaca atgctccccc gtccgacatg cgcaaaacga agcactgccg ggtttcatcc
     8461 ggccataatg gcagcgactc ccgctccccc catcctcgca cctgacagct cggcgcttaa
     8521 gcaaacctta agctcggcgc cctaatctgc acgcctcgca atgcatccca cggaggtaaa
     8581 aatgttacgc aaatttattg tttcaaatct ggcaatggcg gccatcacgg tactgatggc
     8641 ccagccggtt ctcgcggaaa caccggatga aatgctgacc ctcatcaaga aggaagcccg
     8701 tacaggtgac gcttcgttcc agggcttctc cgcatcgcgc ggagaaaaat tcttcaagca
     8761 gaagcaaggg ggcgaatgga gctgcagttc ctgtcacggc gacaatcctg ccgcaaccgg
     8821 caaacatgcc aagaccgata agaccataca gccaatggca cctgcggcca atgcggagcg
     8881 tttcaccgat ccgaagaaag tggcgaaatg gttcaagcgc aattgcaacg atgtgctgaa
     8941 tcgcgtctgc acgccgcagg aaaagggtga tgtgctttcc tatctgctca cggtaaagaa
     9001 ataaggaaac gaccatgaaa tccacttgca caaacattgc caggagactc gcttgggcct
     9061 tgttgatcac aggggccgcg atcaccagcg cccatgccaa acacgacgag aacaaaccgt
     9121 tgactgctgc cggcaacggc cagtggcaag cggagtgcgg ttcctgccat atcccctatt
     9181 ctccccgcct gctacacgca gaatcatggc gggccgtgat gtccggactg gaccgccact
     9241 tcggtacgga cgccagcctg gacgcggcca cgacgcaaca gatcggcagc ttcctggaaa
     9301 gaaatgcggg cagcaggaag gagcctgcct cggcaaagcc ggtcttgcgc atcagcgaga
     9361 cgcgctggtt cattcgcgaa catcgcaaac tgtccagcaa ggtgtggacc agcgacctgg
     9421 tgaaaagccc cgccaactgc accgcctgcc acgccagtga gcccagcggc aactacagcg
     9481 aacgcaatgt cagcgtccca caatagaagg agaacatcat gaatcaacgt atgaaagttt
     9541 gggatctgcc aaccagagtg tttcattggc tgctggcgct ttcttttagc ggcgcatttc
     9601 tgaccgcaga gtccgatcgt tatcgcgaca tccatctggt actcggctat accttgctgg
     9661 gactgatcgc attcagactg gtatggggat tcgtcgggac gcgctatgcg cggttccgct
     9721 ccttcctgtt cggccccggc gaaaccaagg cttatgtgac ctcactgttc aagtcgcagc
     9781 ccaagcatta cgccggtcac aatccggcag gcagcctggc cgtcttcgcc ctgctcggac
     9841 tcggcatcgc ctccggcctg agcggcgtgt tgctgcttca ggatgcgggc ggcgaagcgc
     9901 tggaagaact gcatgaactg gtctcctacg gaatgctggc ggtggtcgtg atccatgtca
     9961 tcggcgtatt gttcagcagc atgatgcacc gtgagaatct ggtgcgcgcg atgatcaccg
    10021 gcttcaaacc cgcacagccg aacgaaggca tccgccgcac gcacgcctgg ctgggtgtca
    10081 taatactcgc ggcggttctg gcattctggt ggggctatcc tgcgaccggt ctggttacgc
    10141 cgaacagcga tgtcgcacaa agccagaacg acgatgacga cgactgagtt ccccgttaac
    10201 ttatcgaaag atgacatgcg catattggta atcgaagacg atgtcctgct gggggatgcc
    10261 attcaggctg gcctgaaaca agccggctat gcggtggact ggatgaaaga cgggataacg
    10321 gccgatcagg cgctcagcac cgagccttat gcggcggccg tgctcgacct gggtctgccg
    10381 cgcatggcgg gactggaggt gttgcggcgg ctgcgcagcc gcaatatacc gataccggtg
    10441 ctgatcctga ccgccatgga taccgtggaa gaccgcatca agggactgga tgcgggagcg
    10501 gacgactatc tggccaaacc gttcgacatg ggcgagcttg ccgcgcgttt gcgggccttg
    10561 ctgcgtcgcg ccagcggcaa gacggcgccc gcactgcaag cagcgggggt ggagctgaac
    10621 cccgccacac atgccgtgtc atatcggggt caggtgatcg atcttcccgc caaggaattt
    10681 gccgtgctgc acgcattgat gctgaatacc ggcagagtgc tgtcgcgcgg gcagatcgag
    10741 gaacagcttt attcatgggg cgaggaagtc gagagcaatg cggtggaagt ctacatccac
    10801 catttgcgtc gcaagctttt cccgggattg attgaaacca tccgtggcgt gggttatctg
    10861 atacccgcag ccaacatttg agatgcgcaa gcacagttcg ttaaaacaaa ggctgctcgc
    10921 actggccctg acgacggtca ttctggtctg ggccggggcg atcaccttta cctatttcga
    10981 tgcgcgcgag gaattcgacg aggtcctcga tgcctacctt gcacaatcgg cctcgctgct
    11041 cgtcattcag gcctcccgcg atctggatga aatcaaaacg gaccatgccc ccctgctgca
    11101 caaatactcg cgccatgtcg cgttccagat ctgggagaat ggacaggtgc tgcgcctgca
    11161 ttcggccaat gcaccggcat ctcccctctc cgttcgcgaa cacgggttca gcgacaccgt
    11221 catcgacggg gaaagctggc gggtgttcag catctgggac gacggcaaaa agaatctgat
    11281 ccaggtggcg gaacgcaccg aagtgcgcga cgaactggcg ggcggcatag cgggcaatct
    11341 gctcacgcca ttgctggttt cactgccatt gctggcattg ctgttgtggg tctccgtgac
    11401 acggggcctg cacccgctgt tccgcctgac cgccgaagtc gcgcagcgcg agccggacaa
    11461 tctggcaccg ctcgagatag caagcgcacc gcgcgaggtc gctccgctga tcgaacgtct
    11521 caacagtctg tttgtccgca tcgatgtatc gctgcaaaaa gagcgccgtt ttactgccga
    11581 tgccgcgcac gaattgcgca cccccgttgc cggtatcaag gcacaggcac aggtggcccg
    11641 cgccgcctcc agcgataaag agcgcattca tgcactcgac aatgccattc tgggctgtga
    11701 ccgcgccgcc catctcatcg accagctgct gaccctcgcg cgcatcgaca ccctggacga
    11761 ggaaatcacc gaggcgtgcc agctcagagc gctcgccgca gaagtgatcg ccgccatcgc
    11821 accgacggca ctggctcagg gggttcaaat cgaattgtcc gaaggcgatg aagtgagggt
    11881 gccgggcagc tcgactttgc tacggatact gctacgcaat ctgatcgaga atgccgtgcg
    11941 ccacacgcca acgggcacgc tggttcaggt cgggatcacc ttgcaacagg ggcatccctg
    12001 cctgtcagtt tgcgacaacg ggccgggcat tgcaaaagcc gacctggaca ggatagcgga
    12061 acggttctat cgccccgttg gaacgagcgc cagcggcagc ggattgggcc tgtctatcgt
    12121 caaaagaatc gctgagattc atgcggcaga actcaaaatc tcacctcgag aaaacggcaa
    12181 gggactggac atctcggtga ttttcaacgc ctgagttgga taggcgaaag aaacaccgcc
    12241 cctgattccg cgcgaccttt aaagctgaaa taccgctcgg cttttatggg tgacacgccc
    12301 ccatcccgac caaaatcccg caaccagcct gccgaatcaa ttaatgatca ggcagttaag
    12361 ctggccgcca ctcttaagca attcttaagg ttggtctttt acactgactg ccccgcaatg
    12421 taaggagact cagtgaagaa gacttatgaa ggtatattga gatttgccag atggacggtg
    12481 gtggggctta tctcgctgat cgtaacgcta ccggcattca cctcggtcgg cgcatccatc
    12541 taagcaccat aaaccacaga cttcgacgca tcagcgtcta ttttttagaa cgaaaatttt
    12601 tgaggacaaa tcatgaaatc tatcatcgtt agtttggctg caacaggctt gctgatcgca
    12661 ggcaatgcaa tggcgatcga aatgccgacg gatggcaaaa ccaagtgcgg cgcctgccac
    12721 aagatcgaca ccaaggcagt gggcccggcc tggaaagacg ttgctgccaa atacaaaggc
    12781 aaggccgatg ccgagaaaac cttgatcgcc aatatcacca agggcggtca gttcggctgg
    12841 aaacagggca acatgccggc aaaaggcatg ggcgccaacg atgcccaaat tgcggctctg
    12901 gctaaattca tcgcagttga tttgaataaa taaccggcgc ttccgcaaca acacagaaaa
    12961 ccggttggtt ccggttttct gtgcctcaat tcagaatctg tgcctcaatt cagaaaccgc
    13021 ctgagcgctc attgccccaa gagttgaata ctccagaacg ccagaaatcc gccgaacagc
    13081 accaaggtag agatcggttt ttcctcgact tcgtgggctt ccatcagcaa ttcttcggtg
    13141 accagataga gcaaggccgc agcactaaag gccaatgcgc cgccgatcac ggcatgcgaa
    13201 gcaccagcca gtagcgcgct gccgatcacg gcaaaggtca gcaccgttcc gcccaacgct
    13261 ccggaaatgg cgatgatacg ccagcccgca gtcgcctctg aagccagggc cagaccgaga
    13321 aacaacagct ctaccgacaa ccccagcgcc aggatcgttc ccgtttctcc gcctgctgcg
    13381 aagcccgcgc cgatgatgaa cccatcggtg gccacatcga taaaggtagc taatagcaaa
    13441 ccggttccca gtcctgccgc cgcaccgcta accgagacct gatgttcgag acgcatcgtc
    13501 cacagcttca acccgtacat gaacagacta cccagcgcga atgaaccgac caagaccaga
    13561 ccgggcgcat gttcacgtcc gatctcgggc agcaactcca ccgccagtgc tgccaatacc
    13621 accccggcag caaagtgctg gatcaggcta cgcatttgat gatttggacg ccagactgcg
    13681 gcaaggattc cgccgccaag agcgactagc gcgggaatcg ccatgatcaa taattcaggt
    13741 gcattcattg tgatgtgtac tcaattacga taaaggtcgt gaatcgtaca gcatgtacga
    13801 taaatgaagc ttgcagctac cacccgaact cgtgtctgcg atgggccgca tctcagtcaa
    13861 acaactcttg ccagagtgat tttttgccac gatagccatg gtgtccatcg tgatgtttgc
    13921 catggtagcc gtgattgttg tcatatggct gctgtaccgg acgggacgac ggcatcaccg
    13981 gtgcgatctg cgccagctcg ctcatgctgc gttcgatgat tttatccagt tcgccacgat
    14041 cgagccagac accccggcac tgtggacaat aatcgatttc gatccccgcg cgttcagtca
    14101 taagcagatt gatgctgggg caggcgggac atttcatgtt gatccttttt aaatagatgg
    14161 ccacaccgga atcgccgcag gtctggactt ggtttgaaaa atatttgttt gaaacgaatc
    14221 atccaccggg cgacagaagg ctgcgatgtc cgtttttttg cagctttccg gtcagtcttt
    14281 cggcgcagtc t
//
LOCUS       DASUIV010000289         8898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1005369, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000289 DASUIV010000000
VERSION     DASUIV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005369"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFW59_10400"
     CDS             complement(<1..450)
                     /locus_tag="VFW59_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364666.1"
                     /translation="MTKAAWNYLKKHLYVRLTWHTLLIGLLIGLLATLYETFEHFDTE
                     RDGRASEIAYLLNVSLRPAARAAVSHDKALADEVAQGLFAQPSIYRVVISDQQGRVLS
                     EVGRSKFEPGKLAQLMFEKEQDYRVSLSVPEDLAGHAAPDAQVGELYV"
     gene            complement(486..1508)
                     /locus_tag="VFW59_10405"
     CDS             complement(486..1508)
                     /locus_tag="VFW59_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HEX5364667.1"
                     /translation="MQPFTAYRIFEDGGKSAGRFVELTLDDLDAGEVVIRTHYSSVNY
                     KDALAATGAGKVIRRFPCVGGVDVSGVVERSVDARFKAGDEVLVTGYGMGVDHDGGYA
                     EYVRVPADWVVPLPEGLTLFDAMALGTAGFTAALAIHRMEQNELRPESGKVVVTGASG
                     GVGSLAIAMLAQRGYHVVAVTGKASEHEYLRSLGASEILPRDQIELTSGRALEKAQWA
                     GALDAVGGDTLAWLTRSMQQNGVIASFGNAGGVELHTTVFPFILRGVRLIGIDSAATP
                     MSQRREIWQRLAGDPSTGLRTGLHPKALSLIAQVVPFAGLSEVFAPLLQGRLRGRTVV
                     AVAEAS"
     gene            complement(1508..2671)
                     /gene="odhB"
                     /locus_tag="VFW59_10410"
     CDS             complement(1508..2671)
                     /gene="odhB"
                     /locus_tag="VFW59_10410"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX5364668.1"
                     /translation="MSIIEVKVPQLSESVTEATLLSWHKQVGDAVSEGENLIDIETDK
                     VVLELPAIKSGVLVAILKQDGEQVGSNEVIAKIDTAAVATTSPAKEGAANQAAAVSPS
                     ARKLAHEHEVDASQLQGSGRHGLVTREDVLSAVQKVPAVELPPVAPVIAPSGNRPEQR
                     VPMTRIRQRIAERLLQSQQNAAILTTFNEVNMQPVIELRNKYKDVFEKKHGVKLGFSS
                     FFVRAAVLALQKFPIVNASVDGNDIIYHGYFDIGVAIGSERGLVVPIIRDADKLSFAE
                     IEKRIADFGVRAKNGKLSMEELTGGTFSISNGGVFGSMLSTPIINPPQSAILGIHATK
                     DRPVAENGQVVIRPMNYLALSYDHRIIDGREAVQFLGTIKEALEYPGRILLDL"
     gene            complement(2658..5570)
                     /locus_tag="VFW59_10415"
     CDS             complement(2658..5570)
                     /locus_tag="VFW59_10415"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5364669.1"
                     /translation="MTAPDENYLRSMQESSLLFGGNNVFVEGLYEDFLRNPASVPDEW
                     REYFGRLIADGKLPDVAHTPVQRSFAAMSVSAGAPQPHADDERKQASVLQLINAYRFL
                     GVRVADLDPLSRHEKPEVAELNPAYYGLGEADMDSTFNTGSLVGGARMTLREVLQRLR
                     RIYCSSVGAEYMYISNVPQKRWIQARLEGAGREPNFSAETKKTILERLTAAEGLERYL
                     HTKYVGQKRFSLEGGETLIPLLHHLLQRAGEQGVKEAVIGMAHRGRLNVLVNTLGKLP
                     KNLFAEFEGVHLEHLTSGDVKYHQGFSSDISTPGGPMHVTLAFNPSHLEIVNPVVEGS
                     VRARQHRRGDHNGKEVLPVLLHGDAALAGQGVNQETFNLSQTRGYGTGGTVHIVVNNQ
                     IGFTTSNALDARSSAYCTDIAKMIDAPIFHVNADDPDAVMMITELAFDFRQQFAKDVV
                     IDMVCFRKLGHNEQDEPLVTQPFMYRYINKHPGTRALYANRLVAQGVLAAEEPEAMVM
                     AYRQAMDEGQNPAHPLRTDFNQERVASWAKFLTGAPWNIAVETAVPLERLQHLAERLT
                     CVQPNFKLHGRVEKIVNDRAAMGRGELPLDWGMAENLAYATLLTEGYPVRLSGEDCER
                     GTFFHRHAVWHDQNRTEWYKGYQIPLQTLEEGQADFLVVNSILSEEAVLAFEYGYATA
                     EPDTLVLWEAQFGDFANGAQVVIDQFIASGEAKWGRLCGLTMLLPHGYEGQGPEHSSG
                     RIERYLQLCADYNIQVCVPSNAAQVFHLLRRQMLRPVRKPLIVFTPKSLLRSKDASSP
                     LQDFTQGGFHPVIGEVEPLDVGAHETSVPAPARRAVPVRRIIACAGKVYYDLIKGRSE
                     RGIDDMVVIRIEQLYPFPHDDFAAQIAAYPNATEVVWCQEEPGNQGAWHRIQHYLLRH
                     LRAGMRLDYALRPSSASPAAGYLAIHNEQQKAVVEAAFRPDLDVKNNPGDRRHEHH"
     gene            complement(5583..6872)
                     /gene="gltA"
                     /locus_tag="VFW59_10420"
     CDS             complement(5583..6872)
                     /gene="gltA"
                     /locus_tag="VFW59_10420"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX5364670.1"
                     /translation="MDKQRLATLTLDDGRSIELPILSGTLGPDVLDMRGLNKLGMFTY
                     DPAFFATASCTSEITFIDGDAGLLYYRGYPIEQLAEKSDFLEVCYLLLNGELPNAVQK
                     EDFSKIITHHTMVHDQLARFFNGFRRDAHPMAVMVGVVGALSAFYHDSLDVNNPKHRE
                     ISAHRLLAKVPTIAAMAYKYNIGETFMHPKNKFSYVENFMYMMFATPAEEYVPNPVLV
                     RALERIFILHADHEQNASTATVRLAGSSGANPFACVASGIAALWGPAHGGANEAALKM
                     LEEIGDVSRVAEYVQGVKDKKYRLMGFGHRVYKNMDPRAKVMRQTCYEVLKELKLENN
                     KLFELAMELERTALSDPYFIERRLYPNVDFYSGIVLRAIGIPTNMFTVIFALARTIGW
                     VSQWNEMVGDVEQKIGRPRQLYRGATQRDFVPLAQRR"
     gene            complement(6897..7145)
                     /locus_tag="VFW59_10425"
     CDS             complement(6897..7145)
                     /locus_tag="VFW59_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX5364671.1"
                     /translation="MRNLERIRWRCRRGLLELDILLGRFIERHYATLNPGERDEFEAL
                     LDMPDNPLWDMIAGRAERGAQEAMPGEQQALLEKIRAS"
     gene            complement(7142..7837)
                     /locus_tag="VFW59_10430"
     CDS             complement(7142..7837)
                     /locus_tag="VFW59_10430"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX5364672.1"
                     /translation="MKFSIYRYNPETDAAPYMQDYELETEPVGKMLLDALLLLKEQDD
                     SLGMRKSCREGVCGSDAMNINGRNGLACITPLAGLKEPVELRPLPGLPVIRDLIVDMT
                     QFFKQYHSIKPYLINNDPPPETERLQSPAQREHLNGLYECILCGCCSTACPSFWWNPD
                     KFVGPAGLLQAYRFIADTRDQATAERLDDLEDPYRLFRCHTIMNCVDVCPKELNPTEA
                     IGHIKDMMVKRVV"
     gene            complement(7848..>8898)
                     /locus_tag="VFW59_10435"
     CDS             complement(7848..>8898)
                     /locus_tag="VFW59_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5364673.1"
                     /translation="FQFHPTGVYGAGVLITEGVRGEGGYLVNKDGERFMPKYAPNAKD
                     LASRDVVSRAMTIEINEGRGCGPHGDHVMLKLDHLGDEVIKQRLPGIREIALKFAHVD
                     PAKAAIPVVPTAHYMMGGIPTNYHGQVVAPYKTGPDEVVHGLYAVGECACVSVHGANR
                     LGCNSLLDLIVFGREAGRQIIEDLKSHPHPKPLPADAAERPLARLARLENQQNGERVS
                     EVGDAMRRAMQKHCGVFRFPELLAEGVVQIKEIAERAANTVIQDKSRVFNTARIEALE
                     LDNLIEVAQATMIAAEARRESRGAHARDDFPLRDDVNWLKHSLYFREGHRLDYKPVRL
                     KPLTVDSFPLKARVY"
BASE COUNT         1692 a         2926 c         2551 g         1729 t
ORIGIN      
        1 aacgtagagc tcgccgacct gcgcatccgg tgccgcatgc ccggccagat cctcgggcac
       61 actcagcgac acccggtaat cctgttcctt ttcaaacatc aattgcgcca gcttccccgg
      121 ctcgaatttg ctgcgaccga cctcgctcag gacacgcccc tgctgatcgg atatgaccac
      181 ccggtagatt gatggctgag caaacaagcc ctgagccacc tcatccgcga gcgccttgtc
      241 atggctgact gcggcgcggg cggcgggcct gagactgaca ttgagcaaat aggctatctc
      301 actggcacgc ccatcccgct cggtatcgaa gtgctcgaag gtttcataca gcgtagccaa
      361 caggccgatc agcaagccga tcaacaaggt atgccaggtc agcctcacgt acagatgctt
      421 cttcaaataa ttccatgctg cttttgtcat ttaaccggtc caagccttca attgataacc
      481 ttgcttcagg aggcttccgc gaccgcaacc accgttcgac ctcgcagcct gccttgcagc
      541 aacggagcga acacttcaga cagaccggca aaggggacga cctgcgcgat cagactcagc
      601 gccttcggat gcaggcctgt cctgagccct gtcgaaggat cgcccgccag ccgctgccag
      661 atctcgcgac gctgcgacat cggggtcgcg gcagaatcga taccgataag acgcacaccg
      721 cgcagaatga aaggaaatac cgtcgtatgc agttcgacgc cgcccgcatt gccaaaactg
      781 gcgatcacac cgttttgctg catgctgcgc gtcagccagg ccagcgtatc gccgcccacc
      841 gcatccagcg caccggccca ctgcgccttc tccagtgcac gtccgctggt cagttcgatc
      901 tggtctcgcg gcaatatctc gctcgccccc agcgatcgca aatattcatg ctcgcttgcc
      961 ttgcccgtga ccgcgacgac gtgatagcca cgctgtgcca gcatggcgat ggccagactc
     1021 ccgactccac cgctcgcccc cgtcaccacc accttgccgc tctccgggcg caactcgttc
     1081 tgctccatgc gatggatcgc cagcgccgca gtgaaacccg ccgtacccag cgccatcgca
     1141 tcgaatagcg tcagcccctc gggcagcggc accacccaat cggcgggaac gcgcacgtac
     1201 tcggcataac cgccatcgtg atccacgccc atgccgtaac cggtgaccag cacctcgtcg
     1261 ccagccttga aacgcgcatc cacagaacgc tcgaccacac ccgacacatc caccccgccc
     1321 acgcagggaa agcggcggat gaccttgccc gcaccggtgg ccgcgagggc gtccttgtag
     1381 ttcacgctgg aataatgcgt gcggatgacg acttcgcctg cgtcgagatc gtccagtgtc
     1441 agttcgacga aacgcccagc cgatttgccg ccgtcctcaa agatgcggta ggcagtaaaa
     1501 ggctgcatca cagatccagc aagatgcgcc cgggatactc cagtgcttcc ttgatggtac
     1561 cgagaaactg caccgcctcg cgcccgtcga tgatgcgatg gtcgtaagac agtgcgagat
     1621 agttcatcgg ccggatcacc acctggccgt tctcggcgac cggcctgtcc ttggtcgcgt
     1681 ggatgccgag gatggcggac tgcggcggat tgatgatggg cgtggacagc atggagccga
     1741 acaccccgcc gttggatatc gagaacgtgc ccccggtgag ctcttccatg ctcagcttgc
     1801 cgtttttggc gcgcacaccg aagtccgcga tgcgtttctc gatctcggcg aacgacagct
     1861 tgtccgcatc gcggatgatg ggcaccacca gaccgcgctc gctgccgatg gccacgccga
     1921 tgtcgaagta accgtgatag atgatgtcgt tgccgtcgac cgacgcattg acgatgggga
     1981 acttctgcaa cgccagcacc gcagcacgca cgaagaacga cgagaagccc agcttcacgc
     2041 catgcttctt ctcgaacaca tccttgtact tgttgcgcag ctcgatcacc ggctgcatat
     2101 tcacctcgtt gaaggtggtc aggatcgccg cgttctgctg cgattgcagc agccgctcag
     2161 cgatgcgctg acgaatacgc gtcatcggca ctcgctgttc gggacggttt cccgacggtg
     2221 cgatcaccgg agcgacggga ggcaattcaa cggcgggcac tttctgcacc gcgctcaata
     2281 catcttcccg ggtcacgagc ccatggcggc cagagccctg caactggctg gcatcgactt
     2341 catgctcgtg cgccagcttg cgcgccgagg gggacaccgc cgcagcctga tttgccgccc
     2401 cctcttttgc aggcgaggtt gttgcgactg cagcagtatc gatcttggcg atgacctcgt
     2461 tgctccccac ctgttcgcca tcctgcttga ggatcgctac aagtacgccg ctcttgatcg
     2521 caggcagctc cagcaccacc ttgtcggtct cgatatcgat caggttctcg ccttcgctga
     2581 ccgcatcgcc gacctgcttg tgccagctga gcaatgtcgc ctcggtaacg gattcggaca
     2641 gctgcgggac tttgacttca atgatgctca tggcgtcgat ctcctggatt gttctttaca
     2701 tctaagtccg gccggaatgc ggcttcgacc acggccttct gttgttcgtt gtggatggcg
     2761 agatatcccg cagctggaga ggcagaggat ggccgcagcg catagtccag cctcatgccg
     2821 gcgcgcaggt gacgcagcag ataatgctgg atacggtgcc acgccccctg gttgcccggc
     2881 tcctcctgac accacaccac ctcggtcgca ttcggatagg ccgcgatctg tgcggcgaaa
     2941 tcgtcgtgcg ggaagggata gagctgttcg atacggatga ccaccatatc gtcgatgcca
     3001 cgctcgctgc gtcccttgat caggtcgtaa tacaccttgc cggcacaggc gatgatgcgg
     3061 cgtacgggca cggcgcgccg tgccggtgcc ggaacagaag tttcatgtgc ccctacatcc
     3121 agcggctcga cttcgccgat gaccggatgg aatccgccct gcgtgaagtc ctgaagcggc
     3181 gaagaggcat ccttgctgcg cagcaggctc ttgggagtaa acacgatcag cggcttgcgc
     3241 accgggcgca gcatctggcg gcgcagcaga tggaacacct gcgcggcatt ggagggcacg
     3301 cacacctgga tgttgtagtc ggcgcacagc tgcagatagc gctcgatgcg gcccgacgag
     3361 tgctccggcc cctgcccttc gtagccgtgc ggcaacagca tggtcagacc gcacaagcgc
     3421 ccccacttgg cctcgcccga ggcgatgaac tgatcgatga ccacctgcgc accgttggcg
     3481 aagtcgccga actgcgcctc ccacagcacc agcgtatccg gttcggcagt ggcgtaaccg
     3541 tattcgaatg ccagcaccgc ctcttccgaa aggatggagt tgacgaccag aaagtccgcc
     3601 tgcccctcct ccagcgtctg caagggtatc tgataaccct tgtaccattc ggtacggttc
     3661 tgatcgtgcc aaaccgcatg gcggtggaag aaggtgccgc gctcgcagtc ctctccggac
     3721 aaacgcaccg gataaccctc ggtcagcagc gtggcatagg ccagattctc ggccatgccc
     3781 caatccagcg gcaactcgcc acggcccatc gcggcacggt cattgacgat cttctcgaca
     3841 cgtccgtgca gcttgaaatt aggctggacg caagtgagcc gctcggccag gtgctgcaga
     3901 cgctcgagcg gcacggcggt ttccaccgcg atgttccagg gggcgccagt caggaacttt
     3961 gcccagctcg ccacccgttc ctgattgaaa tcggtgcgca gcggatgggc cggattctgc
     4021 ccttcgtcca tcgcctgacg ataggccatc accatcgcct ccggctcttc agccgccagc
     4081 acaccctgag ccaccagacg gttcgcatac agcgcacgcg ttcccggatg cttgttgatg
     4141 tagcgataca tgaacggttg cgtgaccagc ggctcatcct gttcgttgtg ccccagcttg
     4201 cggaaacaca ccatgtcgat caccacatcc ttggcgaact gctgacggaa atcgaatgcc
     4261 agttcggtga tcatcatgac cgcatcggga tcatccgcat tcacatggaa gatcggcgca
     4321 tcgatcatct tcgcgatgtc ggtgcagtac gcagaagagc gcgcatccag cgcattcgag
     4381 gtggtgaagc cgatctggtt gttgacgacg atgtgtaccg ttccgccggt gccgtaaccg
     4441 cgcgtctgcg acagattgaa ggtctcctga ttcacgccct gccctgcgag cgccgcgtcg
     4501 ccatgcaaca gcaccggcag cacctcttta ccgttgtgat cgccacgccg gtgctggcgc
     4561 gcacgcaccg aaccttccac gaccggattg acgatctcca gatgagaggg attgaaggcc
     4621 agcgtgacat gcatggggcc gcccggtgtg gagatatccg aggagaagcc ctggtgatac
     4681 ttcacgtcac cggaggtcag atgctccaga tgcacgcctt cgaattctgc gaacaggttc
     4741 ttcggcaact tgcccagcgt gttcaccagc acattcaggc gaccgcgatg ggccatgccg
     4801 atcacggcct ccttcacacc ctgctcgccc gcgcgctgca acagatggtg tagcaacggg
     4861 atcagcgtct cgcccccctc cagcgaaaag cgcttctggc cgacatactt ggtatgcagg
     4921 tagcgctcca gaccttcagc agcggtgagg cgttcgagga tggttttctt cgtctccgcc
     4981 gaaaaattcg gctcccgccc cgcgccttcc agccttgcct gtatccagcg cttctgcggc
     5041 acattgctga tatacatgta ctcggcgccg acgctgctgc aatagatgcg gcgcaggcgc
     5101 tgcaatacct cgcgcagcgt catgcgcgcg ccgcccacca gcgagccggt attgaaggtg
     5161 gagtccatgt cggcctcgcc caggccgtaa taggcaggat tcagctcggc gacttccggt
     5221 ttttcatgac ggctcagcgg atcgagatcg gcgacgcgca ccccgaggaa acgataggcg
     5281 ttgatcagtt gcaacaccga cgcctgcttg cgttcatcgt cggcatgcgg ttgcggtgca
     5341 ccagcactga ccgacatcgc ggcaaaactg cgctgaaccg gcgtatgcgc gacatccggc
     5401 agcttcccgt ctgcgatcag gcgtccgaaa tactcgcgcc attcgtccgg gacgctggcg
     5461 gggttgcgca gaaagtcctc gtacagacct tcgacgaaca cgttgttgcc gccgaacagc
     5521 agcgagctct cctgcatgct gcgcagataa ttttcgtccg gggcggtcat cgtctgttgc
     5581 gactaacgac gttgagcgag cggcacgaag tcgcgctggg tggcgccgcg atacaactgg
     5641 cgcggccgtc cgatcttctg ctccacgtcc cccaccatct cgttccactg cgacacccag
     5701 ccgatggtgc gtgccagcgc gaagatcacg gtgaacatat tggtcgggat gccgatcgcg
     5761 cgcagcacga tgccggaata gaaatccacg ttcggataca gcctgcgctc gatgaaatac
     5821 gggtcgctca aggcggtgcg ttccagctcc atcgccagtt cgaacagctt gttgttctcc
     5881 agcttgagct ccttgagcac ctcataacag gtctggcgca tcaccttggc gcgaggatcc
     5941 atgttcttgt agacgcggtg accgaatccc atcaggcggt acttcttgtc cttcaccccc
     6001 tgcacatatt ccgccacacg cgacacgtcg ccgatctctt ccagcatctt cagcgctgcc
     6061 tcgttcgcgc cgccatgtgc gggcccccac aacgccgcga tgccggaagc cacacaggcg
     6121 aacggattcg caccgctcga cccagccagt cgcaccgtcg cggtcgaggc attctgctca
     6181 tggtcggcat gcaagatgaa gatgcgttcc agcgcgcgca ccagcaccgg attcggcaca
     6241 tattcctcgg cgggcgtggc gaacatcatg tacatgaagt tctccacata gctgaacttg
     6301 ttcttcggat gcatgaacgt ctcgccgatg ttgtacttgt acgccatcgc ggcgatggtc
     6361 ggcaccttgg ccagcaggcg gtgagcggat atctcgcggt gcttgggatt attgacgtcc
     6421 agcgaatcgt ggtagaacgc ggacagtgcg ccgaccacac cgaccatcac cgccatcgga
     6481 tgcgcatcgc ggcggaaacc gttgaagaag cgcgccagct ggtcatgcac catggtgtga
     6541 tgggtgatga tcttgctgaa atcctccttc tggacggcat tcggcaattc tccgttcagc
     6601 aacagatagc agacctccag aaaatcggac ttctcggcca gctgttcgat cggatacccc
     6661 cggtaataca acaaccccgc atcgccatcg ataaaggtga tctcggatgt gcagctggcg
     6721 gtggcgaaga atgcggggtc gtaggtgaac atgcccagct tgttcaggcc gcgcatatcc
     6781 aatacatccg gccccagcgt acccgacaga ataggcagct cgatgctgcg cccgtcgtcc
     6841 agagtcagtg ttgccaatcg ttgcttgtcc atccattact ccctgtcttg cggcgatcaa
     6901 ctcgccctta tcttttccag caacgcctgc tgttcgcccg gcatcgcctc ctgagcccct
     6961 cgctccgctc tccccgcaat catatcccat agcgggttgt cgggcatatc cagcaatgct
     7021 tcgaactcgt cacgctcgcc cggattcagc gttgcgtagt ggcgctcgat gaagcgcccg
     7081 agcagaatat ccagttccag caagccgcgc cggcaacgcc agcgaatgcg ttccagattc
     7141 ctcataccac tctcttgacc atcatgtcct tgatgtggcc gatcgcctcg gtgggattca
     7201 gctccttcgg gcacacatcc acgcagttca tgatggtgtg gcagcggaac agccggtagg
     7261 gatcttccag atcgtccaga cgctcggcgg tcgcctgatc gcgggtgtcg gcgatgaaac
     7321 ggtaggcctg cagcaaaccg gccggaccga cgaacttgtc cgggttccac cagaacgacg
     7381 ggcaggcggt cgagcagcag ccgcacagga tgcactcgta cagtccgttc aggtgctcgc
     7441 gctgcgcagg cgactgcagg cgctcggtct ccggcggcgg gtcgttgttg atcaggtagg
     7501 gtttgatcga gtggtactgc ttgaagaact gcgtcatgtc cacgatcagg tcgcggatca
     7561 ccggcaaccc cggcagcgga cgcagctcca cgggctcctt caatccggcc agcggcgtga
     7621 tgcaggccag cccgttgcgc ccattgatat tcatcgcatc cgaaccgcac acgccctctc
     7681 ggcaggactt gcgcatcccc agactatcgt cctgctcctt cagcaacagc agcgcatcca
     7741 gcagcatctt gccgacaggc tcggtctcca gttcgtaatc ctgcatatac ggcgcagcat
     7801 cggtctccgg gttgtagcga tagatagaaa atttcatcac gactcctcta gtagacacgc
     7861 gccttgagcg ggaacgaatc cacggtcagc ggtttcaggc gcaccggctt gtaatcgaga
     7921 cgatgtccct cgcggaaata caggctgtgc ttgagccagt tcacgtcgtc gcgcaacggg
     7981 aagtcgtcgc gcgcatgggc gcctcggctc tcgcggcgcg cctcggcggc gatcatggtc
     8041 gcctgggcca cttcgatcag gttatccagc tccagcgcct cgatgcgcgc ggtgttgaac
     8101 acacggctct tgtcctgtat caccgtgttg gcggcgcgct cggcgatctc cttgatctgc
     8161 accacgccct cggccagcaa ctcggggaag cggaacacgc cgcaatgttt ctgcatggcg
     8221 cggcgcatcg cgtcgcccac ctcggagacg cgctccccgt tctgctgatt ttccagccgc
     8281 gcgagccgcg ccaagggacg ctcggcagcg tcggccggca agggcttggg atggggatgg
     8341 ctcttcaggt cttcgatgat ctgcctgccc gcctcgcgcc caaagacgat cagatcgagc
     8401 agggaattgc agcccagacg gttcgcgccg tgcaccgaca cgcaggcgca ttcgcccacc
     8461 gcatacaggc cgtgcaccac ttcatccggg ccggtcttgt agggcgcgac cacctggccg
     8521 tgataattgg tcgggatgcc gcccatcatg tagtgcgcgg tgggcaccac cggaatcgcc
     8581 gccttggccg gatcgacatg ggcgaacttc agcgcgatct cgcgtatgcc cggcagacgc
     8641 tgtttgatca cctcgtcgcc gagatgatcg agtttgagca tgacgtggtc gccgtgcggg
     8701 ccgcagccgc gcccctcgtt gatctcgatg gtcatcgcgc gcgacaccac gtcgcggctg
     8761 gccagatcct tcgcgttcgg cgcgtacttg ggcatgaagc gctcgccatc cttattcacc
     8821 agatagccgc cctcgccacg cacaccttcg gtgatcagca cccctgcgcc atacaccccg
     8881 gtaggatgga actggaag
//
LOCUS       DASUIV010000290         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_76058, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000290 DASUIV010000000
VERSION     DASUIV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76058"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFW59_10440"
     CDS             complement(<1..887)
                     /locus_tag="VFW59_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEX5364674.1"
                     /translation="MPIYWNIPLVLLSVLVAVIGSFAALAHAQRMRESSGRAVTLWML
                     AGAFTLALAIWSMHFIGMLAFHLPIRVAFDPSLSFLSILPAIAASFLSFRVLREPSIS
                     AGNILVSGIFMGVGISVMHYTGMAALKLSPPISYDPLIFGMSVAIAVIASWGALLMMY
                     QGEHIRMSKLPRFALGAVIMGSAIAGMHYTAIVGAHIQPGSVCLVDATRGIEPNILAL
                     LVSLISIFWFGGGVLASLFEQRNSQTLSALELAHEERQQLAERQAAEISLSLRDSRES
                     LQRVLDSVAEGIYGVDMDGR"
     gene            1082..>2352
                     /locus_tag="VFW59_10445"
     CDS             1082..>2352
                     /locus_tag="VFW59_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5364675.1"
                     /translation="MSSSTTSLFNTRQSFNLANGKSGTLYSLPALEAAGVGNISRLPV
                     SIRIVLESVLRNCDGKKVTEQHVRELANWKPNAARTEEIPFVVARIVLQDFTGVPLLA
                     DLAAMRDAAAAQGCDPKVIEPLVPVNLVVDHSVQIDHYNRPDALKLNMELEFERNTER
                     YRFMKWGMQAFDTFKVVPPGIGIVHQVNLEYLARGVLQKDGVTYPDTLVGTDSHTTMI
                     NGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVHLKGKLREGVTATDLVLTITELLR
                     KQKVVGKFVEFFGEGTAALTLPDRATIANMAPEYGATMGFFPVDDVTVQFMKNTGRTA
                     DEVDAFESYFKAQGLFGVPADGSIDYSSVVELDLASIEPSLAGPKRPQDRIALPKMKD
                     TFNTLFSKPIAENGFNQPAENLGKRYATGKDG"
BASE COUNT          539 a          765 c          666 g          382 t
ORIGIN      
        1 cgcccgtcca tatccacccc gtagatgcct tcggcgaccg aatcgagcac ccgctgcagc
       61 gattcccgcg aatcgcgcaa cgacagggaa atttccgcag cctgccgctc ggccagttgc
      121 tgccgctcct catgcgccag ctccagcgcg ctcaacgtct gcgagttccg ctgctcgaac
      181 agggaggcga gaacgccgcc gccaaaccag aaaatggaaa tcagcgacac cagcaaggcc
      241 agaatattcg gctctatgcc tcgcgtcgca tcgaccaggc acacgctgcc gggctgaata
      301 tgcgcgccca ctatcgcggt gtaatgcatc ccggcgatgg ccgaacccat gatgaccgcc
      361 cccagcgcaa aacggggcag cttcgacatc cggatgtgct ccccctgata catcatcaac
      421 agcgcccccc atgaggcgat cacggcgatg gcgacggaca tgccgaaaat cagcggatcg
      481 tagctgatcg gtggagacag cttgagcgcg gccatgccgg tgtagtgcat cacgctgatg
      541 cccaccccca tgaatattcc gctgaccaga atgttcccgg cactgatact gggttcgcgc
      601 agcaccctga agctcaaaaa agatgcggcg atagcgggca atatggaaag gaagctaagc
      661 gacgggtcga aagcgacgcg aatcggcaga tggaacgcca gcataccgat gaagtgcatc
      721 gaccagatcg ccagcgccag cgtgaatgcg cccgccaaca tccacagcgt caccgcccgc
      781 ccgctgctct cgcgcatgcg ttgggcatgc gcgagagcag cgaacgaacc gatcactgcg
      841 accagtacgg acaacaacac cagcgggata ttccagtaaa ttggcaaggc tgaattttgc
      901 gggaatgaat atcccgaaat gctaacccaa aacgctcgaa tccggacaac cggattcttt
      961 ccgaacagaa aaaaccgaca ccgcacatgc gatatgcgaa tcatttcgca aatgaggatg
     1021 caagcgaaac gcacaagctc ttataatcgc gcctttcatt ttgtcccgcc ggagttcaat
     1081 catgtcgtcc agtactacca gcctgttcaa cacccgccag tccttcaacc tcgccaacgg
     1141 caagtccggc acgctgtatt cgctgcccgc gctggaagcc gcaggcgtcg gcaatatctc
     1201 gcgtctgccc gtctcgatcc gcatcgtgct ggaatccgtg ctgcgcaact gcgacggcaa
     1261 aaaggtgacc gaacagcatg tgcgcgaact ggcgaactgg aagccgaacg cggcgcgcac
     1321 cgaagaaatc ccgttcgtcg tcgcccgcat cgtgttgcaa gacttcaccg gcgtgccgct
     1381 gctcgccgac ctcgccgcga tgcgcgatgc cgccgcagcc caaggctgcg acccgaaggt
     1441 catcgagccg ctggtgcccg tgaatctggt cgtggatcac tcggtgcaga tcgaccacta
     1501 caaccgcccc gatgcgctga agctgaacat ggagctggag ttcgagcgca acaccgaacg
     1561 ctaccgcttc atgaaatggg gcatgcaagc atttgacacc ttcaaggtcg tgccgcccgg
     1621 catcggcatc gtgcatcagg tcaacctcga atacctggca cgcggcgtgc tgcaaaaaga
     1681 tggcgtgact tatcccgaca cattggtcgg cacggacagc cacaccacga tgattaacgg
     1741 catcggcgtg gtcggctggg gcgtcggcgg catcgaagcg gaagccggca tgctgggcca
     1801 gccggtctac ttcctgacgc ctgacgtggt cggcgtgcat ctcaaaggca agctgcgcga
     1861 aggcgtaaca gcaaccgacc tggtgctgac catcaccgaa ttgctgcgca aacagaaagt
     1921 cgtcggcaag ttcgtcgagt tcttcggcga aggcactgcc gcgctcacgc tgcccgaccg
     1981 tgccaccatc gccaacatgg cgccggaata cggcgcgacc atgggcttct tcccggtcga
     2041 tgacgtgacc gtgcagttca tgaagaacac cggacgcacc gccgacgaag tggatgcctt
     2101 cgagtcctac ttcaaggcgc agggcctgtt cggcgtgcct gcggacggca gcatcgacta
     2161 cagcagcgtg gtggaactcg acctcgccag catcgaaccg tcgctggccg gcccgaagcg
     2221 cccgcaagac cgcatcgcgc tgccgaagat gaaggacacc ttcaacaccc tgttcagcaa
     2281 gcccatcgcc gagaacggtt tcaaccagcc cgccgagaac ctgggcaagc gttatgcgac
     2341 aggcaaggat gg
//
LOCUS       DASUIV010000291         5103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_912377, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000291 DASUIV010000000
VERSION     DASUIV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912377"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFW59_10450"
     CDS             complement(<1..1220)
                     /locus_tag="VFW59_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5364676.1"
                     /translation="MRVAHPSPKTSAPCWHNQSAQAALDALGATASGLATAEAQNRLK
                     LYGPNHLPAARKDGLLIRYARQFDNVLIYVLLASALVTALLAHWVDCGVIVGVVLINA
                     VIGVIQEGKAERALDAIRNMLSLNATVLRDGQRREIPAEQLVVGDIVVLASGDKVPAD
                     LRLIEARQLRIDEAVLTGESEPVEKNLDAVDAKALLGDRACMAYSGTLAVYGQARGVV
                     VATGTATEIGRISKLISSVDSLETPLLRQMAGFGRQLTTAILLLTALIFVFGVWVRGH
                     DWSEMFLAAVGIAVAAIPEGLPAVMTITLAIGVQRMARRNAIIRRLPAVETLGSVTVI
                     CSDKTGTLTKNEMTVQRIITAGQVFEVSGSGYSPQGGFAVGGREVSVEDYPELTELGR
                     AALLCNDAVLHNDN"
     gene            complement(1299..1820)
                     /gene="tpx"
                     /locus_tag="VFW59_10455"
     CDS             complement(1299..1820)
                     /gene="tpx"
                     /locus_tag="VFW59_10455"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX5364677.1"
                     /translation="MSKDISNKTAVTLQGNPVTLYGKFPEVGQTAPAFTLVDKDLKDV
                     TLQSFAGKRKVLNIVPSLDTAVCATSTRKFNEAAGKLDNTVVLVISADLPFAMSRFCV
                     VEGLENVTTLSLMRGRDFMRNYGVKIADTALAGLTARAVLVLDEKDRVLHAELVDDIT
                     HEPNYDAALAALQ"
     gene            complement(2055..4919)
                     /gene="gcvP"
                     /locus_tag="VFW59_10460"
     CDS             complement(2055..4919)
                     /gene="gcvP"
                     /locus_tag="VFW59_10460"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX5364678.1"
                     /translation="MNTDQFVNRHNGSNAQDVQAMLKKIDAPSLDALISQTVPAGIRL
                     QQPLNLPEGMSEHDYLLHLRGIAAKNKLYKSYIGQGYYNTVLPAVIQRNILENPGWYT
                     AYTPYQAEIAQGRLEALLNFQTMVMDLTGMEIANASLLDEATAAAEAMTMLHGLRSRE
                     DAAAGKNSFFVSSECFPQTIELLKTRAQPLGIELVIGDFKTVTLSDKLYGALLQYPAA
                     DGTVHDYADFVARAKANGMTIAVAADILSLVLLTPPGEWGADVVLGSTQRFGVPMGYG
                     GPHAAYFACRDAHKRSMPGRIIGVSVDADGGAALRMALQTREQHIRREKATSNICTAQ
                     ALLAIMAGMYAVYHGADGLRGIARRVHLSAVALAGELKKLGYNVADGAFFDTLKLLHT
                     DNVKIHALAEAAQINFHYANDGLTLSLDETTTTADLNAILAVFAQASDKSAGKAAPVL
                     SAEQVEAQTMAFKQRQSSILGHPVFNRYHSETEMMRYIKRLENKDLSLTHSMISLGSC
                     TMKLNAASEMLALTWPEFANLHPFVPLEQAAGYQELIAGLDAALSEITGFAQMSFQPN
                     SGASGEYAGLLVIQAYHRSRGEAQRNVVLIPSSAHGTNPASAAMCGMNIVVVKCDSRG
                     NIDVEDLRAKAVEHKDVLSCLMVTYPSTHGVYEESIKDITAIIHANGGQVYMDGANMN
                     AQVGLTSPGNIGADVCHLNLHKTFAIPHGGGGPGAGPIGVAEHLTAFLPSHPMVKVGG
                     AQAIHAVSAAPYGSALILLISYGYIKMMGGAGLTEATRMAILNANYIKESLKNHYATL
                     YSGTQGRCAHEMILDCREWKKEGVEVADIAKRLMDFGFHAPTTSFPVVDTLMVEPTES
                     ESKAELDRFCEAMIAIRSEIEDVLTGKVDKKDNILKHAPHTARAVVTSDWTRGYTREQ
                     AAYPLPWVRDNKFWPAVARVDNVYGDKNLVCTCPPVSSYA"
BASE COUNT          863 a         1624 c         1608 g         1008 t
ORIGIN      
        1 ctgttgtcgt tatgcaacac cgcgtcgttg cacagcagcg cggcacgccc cagttcggtc
       61 agttccggat agtcctcgac ggacacttcg cgcccgccga cggcgaatcc gccctgcggc
      121 gagtagccgc tgccgctgac ctcgaacacc tgcccggcgg tgatgatacg ctgcacggtc
      181 atctcgtttt tggtcagcgt gccggtcttg tccgaacaga tcaccgtcac cgatcccagc
      241 gtctccaccg ccggcagccg ccggatgatg gcgttgcgcc gcgccatccg ctgcacgccg
      301 atggccagcg tgatggtcat cacggcgggc agcccttccg gtatagccgc gaccgcgatg
      361 ccgacggcgg cgaggaacat ctccgaccag tcgtggccgc gcacccatac gccgaagacg
      421 aagatgagcg cggtcaacag caggatcgct gtggtcagtt gcctgccgaa gcccgccatc
      481 tggcgcagca gcggcgtttc cagactgtcc acggaggaga tcagcttgct gatgcggccg
      541 atctcggttg ccgtgccggt ggcgaccacc acgccgcgcg cctgcccgta gactgccagc
      601 gtgccggaat aggccatgca ggcgcggtcg ccgagcagcg ccttcgcgtc gacggcatcc
      661 agattctttt ccacgggttc ggattcgccg gtcagcacgg cttcgtcgat gcgcaactgg
      721 cgcgcctcga tcagacgcag gtcggcggga accttgtcgc cggaagccag caccacgatg
      781 tcgccgacca ccagttgctc cgccggaatc tcgcggcgct gcccgtcgcg cagcaccgtg
      841 gcgttcagcg acagcatgtt gcgtatcgcg tccagcgcgc gttccgcctt gccttcctgg
      901 atcacgccga tgacggcgtt gatcagcacc acgccgacga tcacgccgca atccacccag
      961 tgggcgagca gggcggtaac cagcgcggag gccagcagca cgtagatcag cacgttgtcg
     1021 aactggcgcg cgtagcggat cagcaggccg tctttgcgtg cggcggggag gtggttcggg
     1081 ccgtatagtt tcaggcggtt ctgcgcctcg gcggttgcaa gtccggatgc ggtcgcgccg
     1141 agcgcatcca gcgccgcctg cgccgactgg ttgtgccagc agggcgcgct tgttttggga
     1201 gatggatgag cgactcgcat aatcaatcct cgttcaatgg gtcggtggaa tgttacgccg
     1261 gatgggcgtg tccctgaaca gggcgagtcg cggagccgct attgcaatgc cgccagcgcc
     1321 gcgtcgtagt tcggctcgtg ggtgatgtcg tccaccagct cggcgtgcag cacgcgatcc
     1381 ttctcgtcca gcaccagcac ggcgcgggcg gtgagcccgg ccagcgcggt gtcggcgatc
     1441 ttaacgccgt agttgcgcat gaaatcgcgt ccgcgcatca gcgacagcgt ggtgacattc
     1501 tccagccctt cgaccacaca gaaacgactc atcgcgaacg gcaggtcggc ggagatcacc
     1561 agcaccacgg tgttgtccag cttgcccgcc gcctcgttga acttgcgcgt cgaagtcgcg
     1621 cacacggcgg tgtcgagact gggcacgatg ttcagcacct tgcgtttgcc cgcgaagctt
     1681 tgcagagtaa cgtccttcag gtccttgtcc accagcgtga acgccggggc ggtctgcccg
     1741 acttcaggaa atttgccgta cagggtgacc ggattgcctt gcagcgttac ggcggttttg
     1801 ttgctgatgt ctttggacat gatggtctcc ttgaattttc gtcattccgg cggaggccgg
     1861 aatccagcca aaataaacat tccgcgaagc ggacgacatt tgatgttgac ctgcttcgca
     1921 ggtattttta atcaactaga ttccggcctt cgccggaatg acgcagttcg tagcccggat
     1981 gaaacggagt gcaatccggg caccggtttg gaaaacccgg attccgcttg cgctgcatcc
     2041 gggctacctg ttgctcaagc gtatgacgaa accggcgggc aggtacacac cagattcttg
     2101 tcgccgtaca cgttatccac gcgcgcgacg gctggccaga acttgttgtc gcgcacccag
     2161 ggcagcggat atgcggcctg ttcgcgagtg tagccgcgcg tccagtccga tgtcactacg
     2221 gccctcgccg tatgcggcgc gtgcttgagg atgttgtcct tcttgtccac cttgcccgtc
     2281 agcacgtcct cgatctcgct gcggatggcg atcatcgctt cgcagaagcg gtccagctcg
     2341 gccttggatt cgctctcggt cggctcgacc atcagcgtgt ccaccaccgg gaacgaagtg
     2401 gtcggcgcat ggaagccgaa gtccatcagg cgcttggcga tgtcggcgac ttccacaccc
     2461 tccttcttcc attcgcggca gtcgaggatc atctcgtgcg cgcagcggcc ttgtgtgccg
     2521 ctgtacagcg tggcgtagtg atttttcagc gattccttga tgtagttcgc gttcaggatc
     2581 gccatcctgg tcgcctcggt caggcctgca ccgcccatca tcttgatgta gccgtaggag
     2641 atcagcagga tcagcgcgct gccgtaaggc gctgcggaca ccgcgtggat ggcctgcgcg
     2701 ccgccgacct tcaccatcgg gtgcgaaggc aggaaggccg tcagatgttc cgctacgccg
     2761 atcgggcctg cgcccggccc gccgccgccg tgcgggatcg cgaaggtctt gtgcaggttc
     2821 aggtggcaca cgtccgcgcc gatgttgccg gggctggtca ggccgacctg cgcgttcatg
     2881 ttcgcgccgt ccatgtacac ctggccgccg ttcgcgtgga tgatcgcggt gatgtccttg
     2941 atggactctt catacacgcc gtgcgtgctg gggtaggtca ccatcaggca ggacaacaca
     3001 tccttgtgct ctaccgcttt cgcgcgcagg tcttcgacgt cgatgttgcc acggctgtcg
     3061 catttcacga cgacgatgtt catgccgcac atcgcggcgc tcgcaggatt ggtgccgtgc
     3121 gcggaggacg ggatcagcac cacgttgcgc tgcgcttcgc cgcgcgaacg gtggtaggcc
     3181 tgtatcacca gcaggccggc gtattcgccg gacgcgccgc tgttcggctg aaagctcatc
     3241 tgcgcgaagc cggtgatttc cgacagcgca gcatccagac cggcgatcag ctcctgatac
     3301 ccggcggcct gttccagcgg cacgaaggga tgcaggttgg cgaattccgg ccaggtcagc
     3361 gcgagcatct cggaggccgc gttcagcttc atcgtgcagg agcccagcga gatcatcgag
     3421 tgcgtcagcg acaaatcctt gttctccagc cgcttgatgt aacgcatcat ctcggtctcg
     3481 gagtgatagc ggttgaacac cgggtgaccc agaatcgaag actgacgctg cttgaacgcc
     3541 atcgtttgcg cctcgacctg ctcggcactc agcaccggtg cagccttgcc cgccgacttg
     3601 tcagaagctt gggcgaacac cgcgaggatc gcgttgaggt cggcggtcgt ggtggtctcg
     3661 tccagcgaca gcgtcaggcc gtcgttcgcg taatggaagt tgatctgtgc cgcctcggcc
     3721 agcgcgtgga ttttcacgtt gtcggtgtgc agcagcttca gcgtgtcgaa gaacgcgccg
     3781 tcggcgacgt tgtaaccgag cttcttcagc tcgccagcca gcgcgacggc ggacagatgc
     3841 acgcggcgcg cgatgccgcg caggccgtcc gcaccgtggt acaccgcgta catgccggcc
     3901 atgatcgcca gcagcgcctg cgcggtgcag atgttggaag tcgccttctc gcggcggata
     3961 tgctgttcgc gggtttgcag cgccatgcgc aacgcggcgc cgccgtcggc atcgaccgac
     4021 acgccgatga tgcggcccgg catcgagcgc ttgtgcgcgt cgcggcaggc gaaataagcc
     4081 gcgtgcgggc cgccgtagcc catcggcacg ccgaagcgtt gcgtcgaacc cagcaccacg
     4141 tccgcccccc attcgcccgg cggcgtcagc aacaccaggc tgaggatgtc cgccgcaacg
     4201 gcgatagtca tgccgttggc cttggcgcgc gcaacgaagt cggcgtagtc atgcaccgtg
     4261 ccgtcggcag ccggatattg cagcaacgcg ccgtacagct tgtcgctcag cgtgacggtc
     4321 ttgaagtcgc cgattaccag ctcgatgccc agcggctgcg cgcgcgtctt gagcagctcg
     4381 atggtctgcg gaaagcattc gctcgacaca aagaagctgt tcttgcccgc cgccgcgtct
     4441 tcgcgcgaac gcaggccgtg cagcatggtc atcgcctcgg ctgcggcggt cgcctcgtcc
     4501 agcagcgatg cgttcgcgat ctccatgccg gtcagatcca tcaccatcgt ctgaaaattc
     4561 agcagcgcct ccagacgccc ctgcgcgatc tctgcctgat acggcgtgta ggccgtgtac
     4621 cagcccggat tctccagaat gttgcgctgg atcaccgcag gcagcacggt gttgtaatag
     4681 ccctgcccga tgtagctctt gtacaacttg ttcttcgccg caatgccgcg cagatgcaag
     4741 agatagtcgt gctcggacat gccctccggc aggttcagcg gctgctgcaa acggatgccc
     4801 gccgggacgg tctgggatat cagcgcatcc agcgacggcg catcgatctt cttcaacata
     4861 gcctgaacgt cttgcgcgtt cgacccgtta tggcgattta cgaattggtc ggtgttcatg
     4921 ggatcggaga gatgaaaaga attgtaggat gggttgagct ttgcatggcc aatcgactag
     4981 gccaacaaac aacgtcggcc aagtcgctgg tcagcgtagc gatacccatg cggggcatta
     5041 tcgatttttg atgggatcgc tgcgctcaac ccatcctact taatgattta gtgtgcttcg
     5101 ctg
//
LOCUS       DASUIV010000292         9007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1194706, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000292 DASUIV010000000
VERSION     DASUIV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194706"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1684)
                     /locus_tag="VFW59_10465"
     CDS             complement(167..1684)
                     /locus_tag="VFW59_10465"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX5364679.1"
                     /translation="MAVETELKLRISPEQFARLKRHALLKTNQTGRSVTHRLYNVYYD
                     TPKLNLHQSAMALRLRRVGGQWLQTLKGGGDVKAGLHSRNEWEVAVSGEALDFSSPET
                     KELSSHLPARWRKKLRPVFVTDFSRTTRVLDWQGACIELCFDQGEISTEQHSLPICEL
                     ELELKSGESGRLFELALAILEVVPCELEVVNKAEYGFRLLEGYTERPVKGAAPDISAS
                     DGLPEMLQKLIWSCLLHLQSNLRGAMSSDDGEYLHQMRVALRRLRVVLRMAERFRADA
                     QLTALSGAVAALCVALGRIREWDVFIAETLQPMRARMPGHADLQALLALSEQRQTACY
                     EALRGETQARELQRLILRLALWMNGSYWMAGDEVMPQARDFATRQLRKRFRRLASSAE
                     HLDEFDAGRLHALRICAKKLRYSAEFFASLYGKRKPGAFLAALGEVQDVLGQINDVAV
                     AHRLLDDLAAAPELSRHQEAIALAKGWIAHGLAHQLGMLHKTVRRFNEQAVFWEG"
     gene            1744..2259
                     /locus_tag="VFW59_10470"
     CDS             1744..2259
                     /locus_tag="VFW59_10470"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX5364680.1"
                     /translation="MTSVRSFPPIADEHARILILGSMPGKESLRARQYYAHPRNAFWT
                     ILGELTGASPSLPYEARCRMLKSSGIALWDVLASCTRHGSLDADIDPESIHPNDFSAF
                     FETHPLITQVFFNGAMAEQCFRKQVLPQLTHRPLRLLRLPSTSPANASLRDEQKLQAW
                     QAILADSPPDA"
     gene            2421..4841
                     /locus_tag="VFW59_10475"
     CDS             2421..4841
                     /locus_tag="VFW59_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364681.1"
                     /translation="MPISNLMPAELRLSIDPDTLGFSDTSELLEHALTWIGQERAEIA
                     ARFGLGMDQADYNLFVLGEVGSGRSSLLKQAMQAAAAKRSAPPDLCYLHNFDTPERPH
                     ALRLPAGQGRVLRQFMAKMVKTLLTEIPQQLDGPDFKTESELIAKSYKEEEARAYAEL
                     ETLAETRNFTMHRESGHMLFTLRGKKGHAMTEDEVLALPKERRAEIALAEQELRAEIN
                     RYFEKIRPMERVMNEGLAALKRQILKPLLDRELQEIRTALKKQIKDSVKLGIYLDQVV
                     HDILDNLELFKVTDSDDELRKEVLGKVLSRYRVNLVVDNDGLHGAPVIIEDNPLFRSL
                     FGSIEYQSEDDVLMTDFSRIRAGSLHKAHGGFLMLHLRDLLADGLVWEKLRRFLRSGR
                     LQIEEPGTTFAAIAAVSLIPEAVDIDVKIVLIGSRELYYALQEEDPEFARSFRVKVDF
                     ASVFSASDETRRASAIFVAHLCKKLGLPHFSAAAVARLLEDGHREAEDRSRQSAIFAH
                     TEALVMESAALCRARDGHLVKAEDIEAALAARRLRHDYPDQRLRESIAEGDLMISLHG
                     EQVGQLNGLTVVDQGDYRFGFPVRITARTHAGEDGLLNIEREVEMSGPLHDKGVYILQ
                     NYLSALFAHNAPLALNASIVFEQEYSGIEGDSASCAELYALLSSLSGLPLKQGIAVTG
                     ALNQHGDVLPVGGINEKIEGYFRICETAGLDGSQGVLIPHRNRRHLMLDRKIIDAVAQ
                     GLFHIHTAEHVTEGIELLTGHPAGVASNTGNFPRGSVLSHAQRTLLAYRRACQATEHT
                     KLTRKRLH"
     gene            4936..5751
                     /locus_tag="VFW59_10480"
     CDS             4936..5751
                     /locus_tag="VFW59_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEX5364682.1"
                     /translation="MKPLKNTTRKLQNQIEKLLGWTAESQTNLKPGTPYLKERDGILS
                     LHFNTSSIQSEMSIDAPDELVIPYTRDMMSFLLLEPSPKRIAMIGLGGGSLAKYCYRH
                     LPQADITVVEISPDVIALRNEFAIPPDDERFSVLLGDGAAFVRDAGKPFDVLIVDGFD
                     EGGLPAELGSQQFYDDCFSSLTGTGLMVANLWLGHPRYDQCLRRIRTSFGDRVVVINA
                     DDGLNRTAIAVKSTTFPPASSTIRHHAKLLGTHTLNFQGKSNKLVKALADLAM"
     gene            complement(5968..6171)
                     /locus_tag="VFW59_10485"
     CDS             complement(5968..6171)
                     /locus_tag="VFW59_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5364683.1"
                     /translation="MANGTVKWFNDAKGFGFITPDDGSEDLFAHFSAINMSGFKSLQE
                     GQKVTFEVTQGPKGKQASNIQAP"
     gene            6726..7616
                     /locus_tag="VFW59_10490"
     CDS             6726..7616
                     /locus_tag="VFW59_10490"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HEX5364684.1"
                     /translation="MNLLSVAIEDIPLNSPLPWRLYDRHGYIVFARGEVVSSRKQLES
                     LIPGGLLRDIDVPPQTDETGDWAEFKERLPGGVFPPRGIKPQIGERLQLRLLDQRQQP
                     YYSASLIGYIKKQSLLVTRPQSATNPFIPVEGKQVEVRMVTGNNIYAFQSTIQRLCIS
                     PSHYMHLDFPAEVHVQQLRRSPWARVSIGIIVTDVRGVREAARLVNLSPDGGQLLAPP
                     TLGYAGETLRISFHAAMDELKTTLNLEATIQHVYEAKAGRDTEANMLEYGIAFQNLAT
                     AEALWLQALVYRHIAEGGLA"
     gene            complement(7707..8822)
                     /gene="ald"
                     /locus_tag="VFW59_10495"
     CDS             complement(7707..8822)
                     /gene="ald"
                     /locus_tag="VFW59_10495"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5364685.1"
                     /translation="MKIGIPKEIKPNENRVSVVPSGVEALVSAGHQVIVETGAGHGSG
                     FSDAQYQVAGAQIADDADAVWAVSELVVKVKEPIASEWPRITSEHVIFTYFHFAASLP
                     LTKALMASGATCIAYETVELPSGELPLLTPMSEVAGRMAVQEGAKYLEALHGGRGILL
                     GGVPGVSPAKVVILGGGVVGMNAARMAAGLGAHVILLDSSLERLRHLSEVMPANVQLV
                     FSNRHNLLEQIASADLVIGAVLIHGAVAPKLIRREDLRTMRRGAVIVDVAVDQGGCVE
                     TMHATTHEDPTFVVEGVIHYGVTNMPAGVPITSTLALTNATLPYVLKLASHGCRNALK
                     ASPALLKGLNIMGGEITHKKVAEAFGLDHLPAESMLD"
BASE COUNT         1937 a         2877 c         2508 g         1685 t
ORIGIN      
        1 gaaggcttgc gaggctggat ggataaatgg gtcggcgaat ggttcgtcaa tcacgtcagc
       61 acgatggact tcatgacggc gaggttcatt gcggcaacgc aggggtaatg gtcggcaggt
      121 tgggttgagc gcagcgatac ccaacctacg agtacaccta caaccctcac ccctcccaga
      181 acaccgcctg ttcattgaac cgccgcaccg tcttgtgcaa catgcccaac tggtgcgcca
      241 ggccatgcgc gatccagccc ttggccagcg cgatcgcctc ctgatgcctc gacagctccg
      301 gtgctgcggc caagtcgtcc agcaggcgat gggcaaccgc gacatcgttg atctgcccca
      361 acacatcctg cacctcgccc aacgcagcca gaaacgcccc gggcttgcgc ttgccgtaca
      421 gactggcgaa gaattccgcg ctatagcgga gcttcttggc acagatgcgc agggcatgca
      481 gccgcccggc gtcgaactca tcgagatgct cggccgagct cgccagacgg cggaatcgct
      541 tgcgcaactg acgggtggca aaatcgcgcg cctgcggcat gacctcgtcg cccgccatcc
      601 aataggagcc gttcatccac agtgccagcc gcaagatcag gcgttgcaac tcgcgcgcct
      661 gagtctcgcc gcgcagcgcc tcgtaacagg cggtttgacg ctgttcgctg agcgccagca
      721 aagcctgcag atcggcatgg ccgggcatgc gcgcacgcat cggctgcagg gtttccgcga
      781 tgaacacgtc ccactcgcgg atgcgtccga gcgcgacgca caatgcggca accgcaccgc
      841 tcaaggcggt cagctgtgcg tcggcacgga agcgctctgc catgcgcagc actacccgca
      901 agcggcgtag cgcgacgcgc atctggtgca gatactcgcc atcgtcgctg ctcatcgcgc
      961 cgcgcagatt gctctgcaga tgcaacaggc acgaccaaat cagtttttgc agcatttccg
     1021 gcaagccgtc gctcgcagag atgtccgggg ccgcgccctt gaccggcctc tcggtgtagc
     1081 cctccagcag acgaaacccg tattccgctt tgttgacgac ttccagttcg caaggcacga
     1141 cctccagtat cgccagcgcc agctcgaaca accggccgct ctcgccggac ttcaactcca
     1201 gctccagttc gcagatcggc aggctgtgct gctcggtgct aatctcgccc tgatcgaagc
     1261 acagttcgat acacgcgccc tgccagtcca gcacacgggt cgtccgggaa aagtcggtga
     1321 cgaagaccgg gcgcagcttc ttgcgccagc gcgcgggcag atgcgagctc aattccttcg
     1381 tttcgggcga ggagaaatcc agcgcctcgc cgctcaccgc cacctcccac tcgttgcgac
     1441 tatgcagccc cgccttgaca tcaccgccgc ctttcagcgt ctgcaaccat tgcccgccga
     1501 cgcggcgcag ccgcaacgcc atcgcggatt gatgcagatt cagtttgggc gtgtcgtaat
     1561 agacgttata gagacgatgg gtgaccgaac gtccggtctg gttcgtcttg agcagcgcat
     1621 gacgcttcag ccgggcgaac tgttcggggc tgatgcgtaa tttgagttcg gtttcgacgg
     1681 ccatgaccac ctcatgcgta gcattgatgc gctaactata tcaggctgcg ctatagtttc
     1741 gcgatgacaa gcgtccggag tttccccccc atcgcggatg aacatgcgcg catcctgatc
     1801 ctcggcagca tgccgggcaa ggaatcgctg cgggcgaggc agtattacgc ccacccgcgc
     1861 aacgccttct ggaccatact cggcgagctg accggcgcat ccccctccct gccctatgag
     1921 gcgcgatgcc ggatgctgaa gtcctccggc atcgcgctgt gggatgtgct cgcctcctgc
     1981 actcgccacg gcagcctgga tgccgacatc gatccggagt cgatccaccc caacgacttt
     2041 tcggcattct tcgagacgca tccgctgatt acgcaggtat tcttcaacgg cgcgatggcc
     2101 gaacaatgct tccgcaaaca ggtgctgccg cagctaacac accgtccgct acggctgctg
     2161 cgactgccgt cgaccagccc cgccaacgca tccctgcgcg atgaacagaa gctgcaggca
     2221 tggcaggcca tcctggccga ctcaccacca gatgcatgac cgtcgcgaaa cctctccaga
     2281 taccgccatt tggcccgcaa tattccatgc ctgcccagaa aaaatgcccg cgcaagacag
     2341 ttgccacacc atgccataat gcgtctaatc aaagacagac cagacttacg ccagccagat
     2401 aactgcgaaa acgaatcgac atgccaatct caaacctgat gcctgctgag cttagactca
     2461 gcatcgatcc cgacaccctc ggattttccg acacctccga attgctggaa cacgccctga
     2521 cctggatagg ccaggagcgc gcagagatag cggcgcgttt cggtctcggc atggatcagg
     2581 cggattacaa cctgttcgtg ctgggagaag tcggcagcgg ccgctcctcg ctactcaagc
     2641 aagccatgca ggctgccgcc gcgaagcgat cggctccgcc cgacctgtgt tacctgcaca
     2701 acttcgacac gccggaacgt ccccacgcac tgcgcctgcc cgccggacaa ggacgcgtgc
     2761 tgcggcagtt catggcaaag atggtgaaga ccctgctgac agaaatacct cagcagctgg
     2821 acggccccga cttcaagacc gagagcgagc ttattgccaa gtcctacaaa gaggaagaag
     2881 ccagagccta cgccgaactc gagactctgg ccgaaacgcg caacttcacc atgcatcgcg
     2941 aatccggcca catgcttttt acgctgcgcg gcaagaaagg ccacgcgatg acggaggacg
     3001 aggtgcttgc cctgcccaag gagcgccgcg ccgaaatcgc gctggccgag caggagctgc
     3061 gcgccgagat caaccgctat ttcgagaaga tccgcccgat ggaacgggtg atgaacgaag
     3121 gcctcgcggc gttgaagcgc cagatcttga agccgctgct ggatcgcgaa ttgcaggaga
     3181 ttcgcaccgc cctgaagaaa cagatcaagg actcagtcaa gctgggcatc tatctggatc
     3241 aggtcgtgca cgacatactc gacaatctgg agctgttcaa ggtcaccgac agcgacgacg
     3301 agttgcgcaa ggaggtgctg ggcaaggtgc tgtcgcgcta ccgggtcaat ctggtggtag
     3361 acaacgatgg gctgcacgga gcgccggtga tcatcgaaga taatcccctg ttccgctcgc
     3421 tgttcggcag catcgaatac caatccgaag acgatgtgct gatgacggac ttctcccgca
     3481 tccgcgcggg cagcctgcac aaggcgcacg gcggtttcct gatgctgcat ctgcgcgacc
     3541 tgctggccga cgggctggtg tgggaaaaac tgcggcgctt tctgcgcagc ggccgcttgc
     3601 agatcgaaga acccggcacg accttcgcgg cgattgccgc cgtttcgctg atccccgaag
     3661 ccgtcgatat cgatgtcaag atcgtgctga tcggctcgcg cgaactgtac tacgccttgc
     3721 aggaggaaga cccggaattc gcccgcagct tccgcgtcaa ggtggacttt gccagcgtct
     3781 tctcggccag cgacgaaacc cgccgcgcct cggccatctt cgtcgcccac ctctgcaaaa
     3841 aactcggact accgcacttt tccgccgctg ccgtggcacg cctgctggaa gatggacatc
     3901 gcgaggcgga agaccgctcg cgtcaaagcg cgatcttcgc ccacaccgag gcgctggtga
     3961 tggaaagcgc cgcgctgtgc cgcgcgcgcg acggccatct ggtcaaggca gaggatatcg
     4021 aagccgcgct ggcggcacgc agactgcgcc acgactaccc ggaccagcgc ctgcgcgaat
     4081 ccatcgccga aggcgacctg atgatttcat tgcatggcga gcaggtcggt cagctcaacg
     4141 ggctgaccgt ggtggatcag ggggattacc gcttcggctt cccggtgcgc atcaccgccc
     4201 gcacccatgc cggcgaagat ggactgctca acatcgaacg cgaagtggaa atgtccggcc
     4261 ccttgcacga caagggggtg tacatattgc aaaactattt gtccgcgctg tttgcccaca
     4321 acgccccgct cgcactcaat gcctccatcg tattcgagca ggagtacagc ggcatagagg
     4381 gcgactcggc ctcctgcgcc gagctgtatg ccctgctctc atcgctgtcc gggctgccgc
     4441 tcaagcaggg catcgccgtg accggcgcac tcaaccagca tggcgatgta ttgccggtgg
     4501 ggggcatcaa cgaaaagatc gagggctatt tccgcatctg cgaaactgcc ggactggacg
     4561 gcagtcaggg cgtgctcatc ccgcatcgca accgccgcca cctgatgctg gatcgcaaga
     4621 tcatcgatgc cgtcgcacaa ggcctgttcc atatccacac ggcagaacat gtgacggaag
     4681 gcatcgaact gctcacaggg caccccgccg gcgtcgccag caacaccggc aacttcccgc
     4741 gcggcagcgt gctgagccac gcccagcgaa ccctgctggc ctatcgtcgc gcctgtcagg
     4801 caacggagca taccaagctg acacgcaaac gtttgcactg acctcatgca aaggccgttg
     4861 tcgaggcgat ggcggaagag tatcttcccc gtcgcgatca tgcagggcat accactctca
     4921 caggaaaaaa cgtagttgaa accgctaaaa aacacgaccc ggaagttaca aaaccagatt
     4981 gaaaaattgc ttggctggac agccgaatcc cagaccaatc ttaagccggg cacgccctat
     5041 ctgaaagagc gcgacggcat tctttcgctg cacttcaata ccagttcgat acaaagcgag
     5101 atgtccatcg atgcgccgga cgaactggtc attccctaca cccgggacat gatgagcttc
     5161 ttgttgctgg agccctcgcc caagcgcatc gccatgatag ggttaggcgg cggatcgctg
     5221 gccaaatatt gctaccgcca tctgccgcag gcggatatca ccgtagtcga aatcagtccc
     5281 gatgtgatcg cattgcgcaa tgaatttgcc atcccccccg acgatgagcg ctttagcgta
     5341 ttgctgggcg acggtgccgc gtttgtgcgc gatgcgggca aaccgttcga tgtgctgatc
     5401 gtagatggat tcgatgaggg cgggttgcct gccgaattgg gctcccagca gttctacgac
     5461 gactgttttt cttcgttgac cggaaccggc ctcatggtcg ccaacttatg gctcggtcac
     5521 cctcgctacg atcaatgcct gcggcgcatc cgcaccagtt ttggcgaccg cgtcgtggtc
     5581 atcaatgcag acgacggcct caacagaacg gccatcgccg tcaaaagcac aacgttcccg
     5641 cccgcgtcct ccaccatacg gcaccatgcg aagttactcg ggacacatac gctgaacttt
     5701 cagggcaagt ccaacaagct ggtcaaagcc ttggccgacc tcgccatgtg atactcatca
     5761 tcgcttgccg cccccgaact catccagacg accggggttc agcagatgtt gaccttgcca
     5821 agattgcgat catctcccaa tgaccgcgct ccccggataa aaagagcagg tcgggtgcat
     5881 gccgatttat cctgactgac ccgtgaccga ttttcgaacg aaaaaaaacc cggcataacc
     5941 gggcttctta atcaactcaa gaaccgatta gggagcctgg atgttggatg cctgcttgcc
     6001 ctttgggcct tgcgtaactt caaaggtgac cttttggcct tcctgaagtg acttgaaacc
     6061 gctcatgttg atggcggaaa agtgcgcgaa caaatcttcg ctgccatcat caggagtgat
     6121 aaaaccaaaa ccttttgcat cgttgaacca cttaactgta ccgtttgcca tttgctcgat
     6181 cctttactta aaaaacaggg aaaacaccct ggaaaatttt tacaacgact tcaattgcct
     6241 agactgaaaa ctacttagac ccatccatca taacatcggg tactccgttg actacgtcaa
     6301 tatcacgagt atctgctgag tgacgtgcta tataccttgc acgcaactca ttaaactttg
     6361 gagattgggg tgaggaagaa gagcctgccg acagcactcc gagcgaaccg ctcacgatag
     6421 caggatagga acaaggggca tttacaacgt tctgaaccaa cgacatgaga cactatcacc
     6481 aagtatgcaa aatgcccctg ttccaagcag gcgaaggcat actacgcctt attgggaata
     6541 atcatatagg cacaaatatc tatgcatttt ggcctagtca tattgaatga cggccgagtt
     6601 gagcctgcgg atagtcaaac cggagaaacg gtgaatcgcg agcggcacaa ggctaagcgg
     6661 cggcattcgt gcggccgaag agtgctagaa tcccgggcag tcgtcaacct gccggatgcg
     6721 cccacatgaa ccttctgtct gtcgctatcg aagacattcc ccttaactct cctctgccat
     6781 ggcgactgta tgaccgccac ggttatatcg tgtttgcccg aggcgaagtg gtaagcagcc
     6841 gaaagcaact ggagagcctg attccagggg gactgttacg ggacatcgat gtgccgccgc
     6901 agaccgacga aacaggcgac tgggccgaat tcaaggagcg tctgcccgga ggcgtattcc
     6961 ccccccgagg tatcaaaccg caaataggcg aacgtttgca gcttcgactg ctggatcagc
     7021 gtcaacaacc ctattattcc gcaagcctga tcggctatat caagaagcag agcctgctgg
     7081 tgaccagacc ccagtccgcc accaacccct tcattccggt agagggcaaa caggtcgaag
     7141 tgcgcatggt caccggcaac aacatctatg ccttccagtc cacgatccag cgcctgtgca
     7201 tcagccccag ccattacatg catctggatt ttccggcgga ggtgcatgtg caacaactgc
     7261 gaagatcccc ctgggcacgg gtcagcatcg gcatcatcgt gaccgatgtt cggggtgttc
     7321 gcgaagcggc ccgcctcgtc aacctgagcc cggacggtgg gcagctcctt gcgcccccta
     7381 cactgggcta tgcaggagaa accttgcgaa tttcttttca cgcggcgatg gatgaactga
     7441 agaccacgct gaatctcgaa gcgacgattc agcatgtgta tgaagcgaaa gccggacgag
     7501 acacggaagc caacatgctg gaatacggca tcgccttcca gaaccttgcc accgccgagg
     7561 cgctctggct gcaggcactg gtctatcggc acatcgccga agggggcctg gcctgacccc
     7621 atctgacagc ggttgtttta gcacccacct atcgtgaccg cagcccctgc ccggtcagtt
     7681 gacataagct atccggcgcg gctggctcaa tcgagcatgg attctgccgg gaggtgatcc
     7741 agtccgaagg cttcggcgac tttcttgtgg gtgatttctc cgcccatgat attgagtccc
     7801 ttgagcagcg ccggggatgc cttaagcgca ttccggcatc cgtggctcgc cagcttcagc
     7861 acatagggca aggtggcatt ggtcagcgcc agcgtggaag tgatcggcac gccagcaggc
     7921 atattggtca ccccgtagtg gatcacccct tccacgacaa aggtcggatc ctcatgggtg
     7981 gtcgcatgca tggtctcgac acaccccccc tgatcgacgg ccacatccac gatcaccgcg
     8041 ccccggcgca tggttctgag gtcttcccgg cggatcagtt tgggtgcgac ggcgccgtgg
     8101 atcagcaccg cgccgatgac cagatcggcg ctcgcgatct gctccagcag attgtgccgg
     8161 ttcgaaaaca ccagctgtac attggcgggc atcacctcgc tcagatgacg caagcgctcc
     8221 agtgaggaat cgaggaggat gacatgggcg cccagcccgg ccgccattct ggccgcattc
     8281 atgccgacta cgccgccgcc gaggatcacc accttggcgg gcgacacgcc cggcacgccc
     8341 cccagcagga tgccgcgccc gccatgcagc gcctccagat atttggcgcc ttcctgcacc
     8401 gccatgcgcc cggccacttc cgacatcggc gtcagcaacg gcagctcgcc cgacggcagt
     8461 tcgaccgtct cgtaggcgat acaggtcgca cccgaggcca tcagcgcctt ggtcaacggc
     8521 agactggcgg cgaaatgaaa gtaggtgaag atgacatgct ccgatgtgat acgcggccat
     8581 tccgacgcaa tcggctcctt caccttgacg accagttcgg agacggccca gaccgcatcc
     8641 gcatcgtcgg cgatctgcgc acccgccacc tgatattgcg catcggagaa cccgctgcca
     8701 tgacccgctc ccgtttccac gatgacctga tgcccggccg acaccagcgc ctcgacaccg
     8761 gacggaacca ccgacacgcg gttttcgttg ggtttgatct ctttcggtat gccgattttc
     8821 atgttgcgtc ccttgcaata cggtaaaaac ctgtctctcc gctacagcag caccgcactg
     8881 tccggcatct cgttcggctt gccattgcgc ggcggaaaat ggcgattgag atgatccccg
     8941 accgcgcgta tccccgcaag cgcgccggcc tcgaaacgac cttcgcggaa cgccgtctcc
     9001 atctcgc
//
LOCUS       DASUIV010000293         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1378492, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000293 DASUIV010000000
VERSION     DASUIV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378492"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1823
                     /locus_tag="VFW59_10500"
     CDS             69..1823
                     /locus_tag="VFW59_10500"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5364686.1"
                     /translation="MKHEKATFTELEMFFHTILLVDDSPVNLSVIVDGLEGHGFDVVV
                     ALDGEEALQRVEVVRPDLILLDVMMPGMDGFEVCRRLKAQERTRDIPVIFMTSLSSAE
                     AKITGFAAGAVDYVTKPLQIGEVRARVSTHLALHDLERRLAEKVAVQADHLDRLHEEH
                     SIAAGYMNKLIALDKLDDPAVKFHLQPTEHFSGDLIAAARHPDGRLHLMLADSTGHGL
                     SAALAAMPVIQPFYSMTGKGYGISAIAREINRKVKEVLPVSHFVAAILVSIDADGQMV
                     EVWSGGCPPPLMLDGAGRVAHRFKARHLAMGILPPEQFDSSLEYFSYDDDAHSLLMFS
                     DGVLEQENASGEAFGLARLLSAAESADRDARWQAMIGAIERHCGTGTFSHDDITLMMA
                     RCELHGKFTGECVTRPHGLRASGSGTIGWQFALTLEMAQLRHLDVVPLLLGILQQIET
                     DKERDGEIFLILSEMFNNALDHGVLKLDSGLKHHEDGMEKYFDERSARLANTEVGQIH
                     LHLKKIQNADGSEFLRIRMKDSGEGFDHRQRNVQFHDCTLRHGRGIALLYKVCRSVQY
                     LGNGSELVVEFDLTAAQN"
     gene            1842..>3167
                     /locus_tag="VFW59_10505"
     CDS             1842..>3167
                     /locus_tag="VFW59_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5364687.1"
                     /translation="MRIENSPSIGTNPHLASILLVDDSAVNLGVVVEGFESQGYDVLV
                     AMDGEEALQRVELAMPDLILLDVMMPGMDGFEVCRRLKAQERTRDIPVIFMTSLSGID
                     DKMQGFAAGAVDYITKPLQVNEARARVHTHLELRALRQRLETKNHHMQLEIAERQKAE
                     EALRDKIAHISALNERLEEQAVELEASQEQLRLTEAWYRGIVRSAPDGMMVVDAQGRI
                     SLVNEHLEKVFGYAEGELIGQPMEILLPPDTRGSHVAKRDGFFAGGTMGRPMDAIDSG
                     FRGCRKDGTEFPVDVSLSRLPPAEDRSGVICAAIRDITERRRMEDALAIREQEFRTLL
                     ENTPDVVVRYDLKCRRTYVNPAFVQVNGFSAQEIIGKTPQELSSRIKPVAARYEQMLR
                     GVMTSGRPDSMDLAWMDEAGHQVCFEQNAIPEFDSNGKVVSVLTVARDIS"
BASE COUNT          665 a          840 c         1052 g          610 t
ORIGIN      
        1 aaaaacttcc agaccaaggc actgctgggc ctgatagagc aataccgcag cgaacaggaa
       61 atcgaatcat gaagcatgaa aaagcaactt ttaccgaact ggaaatgttt ttccatacca
      121 ttctgctggt agacgattcg ccggttaatc tgagcgtgat cgtcgacggg ctggaggggc
      181 acggtttcga tgtggtggtg gccctggacg gcgaagaagc cctgcagcgg gtcgaagtgg
      241 tcaggcccga tctgatcctg ctcgacgtga tgatgccggg catggatggc ttcgaggttt
      301 gccggcggtt gaaggcgcag gagcgcaccc gggacatccc ggtgatcttc atgacttcgc
      361 tgagcagcgc cgaagccaag atcaccggat tcgccgcagg ggcggtggat tacgtgacca
      421 aaccgttgca gatcggcgag gtgagggcac gggtcagtac ccatctggcc ttgcacgacc
      481 tggagcggcg tctggcggaa aaggtcgcgg tgcaggcaga ccacctcgat cgcctgcatg
      541 aggaacacag tatcgctgcg ggctatatga acaagttgat cgcgctggac aagctcgacg
      601 atccggcggt gaagttccat ttgcaaccca ccgagcactt cagcggcgat ctgatcgcgg
      661 cggcgcgcca ccccgatggc cgtctgcatc tgatgctggc ggacagtacc gggcacggat
      721 tgtcggccgc actggcggcg atgccggtga tacagccgtt ctattcgatg accggcaagg
      781 gttacggcat ttcggccatt gcccgggaaa tcaaccgcaa ggtcaaggaa gtcttgccgg
      841 tgagccattt cgtggcggcg attctggtgt ctatcgatgc ggacgggcaa atggtcgagg
      901 tgtggagcgg cggatgcccg cccccgctga tgctggatgg cgcaggccgg gtcgcccacc
      961 ggttcaaggc gcgccatctg gccatgggca tcctgccgcc ggaacagttt gactcctcgc
     1021 tggaatattt ttcctatgac gacgatgcgc acagtctgct gatgttttcg gatggcgtgc
     1081 tcgaacagga gaacgccagc ggcgaggcgt tcggtctggc gcgcttgttg tccgccgccg
     1141 agtctgcgga tcgcgatgcc cgctggcagg cgatgatcgg cgcgatcgag cgccattgcg
     1201 gaaccgggac gttcagtcac gacgatatca ccctgatgat ggcgcgctgc gagttgcacg
     1261 gcaaattcac cggcgagtgt gtgacgcggc cgcatggcct gcgcgcatcg ggtagcggaa
     1321 caatcggctg gcaattcgcc ttgacgctgg aaatggcgca actgaggcat ctggatgtgg
     1381 tgcccctgct gctgggaatt ttgcagcaga tcgaaaccga caaggagcgc gacggcgaga
     1441 tattcctgat cttgtccgag atgttcaata acgcattgga tcacggggta ttgaaactgg
     1501 actccgggct gaagcatcac gaagacggca tggaaaaata tttcgacgag cgcagcgcca
     1561 gactggccaa caccgaggtc gggcagatcc acctgcatct gaaaaaaatt cagaacgctg
     1621 acggcagcga attcctgcgt atccggatga aagacagcgg cgagggcttc gatcaccggc
     1681 agcggaatgt gcagttccat gactgcaccc tgcgccacgg acgcggcatc gcgctgctat
     1741 acaaggtgtg ccgttcggtt caatacctgg gcaacggctc ggaattggtg gtcgagttcg
     1801 acctgacggc agcacagaat taatcggagg ataggatggg tatgagaatc gaaaattcac
     1861 cttcaatcgg gacgaaccct catctggcca gcatcctgct ggtggatgat tcggcggtga
     1921 atctgggcgt ggtggtcgaa ggcttcgagt cgcagggata cgacgtgttg gtggcgatgg
     1981 acggcgagga ggcgctgcaa cgcgtcgaac tcgccatgcc cgatctgatc ctgctcgatg
     2041 tgatgatgcc gggcatggat ggcttcgagg tttgccgtcg cttgaaagcg caggaacgaa
     2101 cgcgcgatat tccggtgatt ttcatgactt cgctttccgg tatcgacgac aagatgcaag
     2161 gattcgcggc gggcgcggtg gattacatca ccaaaccctt gcaggtcaac gaggccaggg
     2221 cgcgggttca tacccatctg gaactgcgcg cgttgcgaca gcggctggag acgaagaatc
     2281 accatatgca actcgagatc gccgagcgcc aaaaggccga agaggctttg cgcgacaaga
     2341 tcgcgcatat ttccgcgctg aacgagcgtc tggaagagca agcggtcgag ctggaggctt
     2401 cacaggagca gctcaggctc accgaggcct ggtatcgcgg catcgtccgt tccgcgcccg
     2461 acgggatgat ggtggtcgat gcgcagggac ggatttccct ggtcaatgag catctcgaaa
     2521 aagtgttcgg ttacgcagaa ggggaattga tcgggcagcc gatggagata ttgttgccgc
     2581 ccgatacgcg gggaagccat gtcgccaaac gggacggatt cttcgcgggg gggacaatgg
     2641 ggcgtccgat ggatgccatc gacagcggct ttcgtggctg ccgcaaggat ggcaccgagt
     2701 tcccggtgga tgtgagtctg tcgcgcctgc cgcctgccga ggaccggagt ggggtgattt
     2761 gtgccgcgat acgcgacatc accgagcgcc gccggatgga agatgcgctg gcgatacgcg
     2821 agcaggagtt ccgcacgctg ctggaaaata cccccgacgt ggtggtgcgc tacgacttga
     2881 aatgtcgtcg cacttacgtc aatccggcgt ttgtacaggt taacgggttt tcggcgcagg
     2941 agatcatcgg caaaacgccg caggaactgt catcgcgaat caagcctgtt gctgctcgtt
     3001 acgagcagat gctgcgcggg gtgatgacga gcggacggcc tgactcgatg gatttggcct
     3061 ggatggacga agcgggccat caggtctgtt ttgagcagaa tgcgattccc gaattcgaca
     3121 gcaacggcaa agtcgtcagc gtgctgacgg tggcgcgcga catcagc
//
LOCUS       DASUIV010000294        14393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_913239, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000294 DASUIV010000000
VERSION     DASUIV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913239"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1280)
                     /gene="tyrS"
                     /locus_tag="VFW59_10510"
     CDS             complement(60..1280)
                     /gene="tyrS"
                     /locus_tag="VFW59_10510"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5364688.1"
                     /translation="MTETKHMEPFASALDTIKRGAHELLLEGELKQKLALGRPLRIKA
                     GFDPTAPDLHLGHTVLLNKMRQLQDLGHHALFLIGDFTGMIGDPTGKNATRPPLTREQ
                     VLENAQSYRDQVFKVLDPAKTEVVFNSTWMDKFSAVDLIRLAATHTVAQMLERDDFSK
                     RYKGNQPIAIHEFIYPLVQGYDSVALKADIELGGTDQKFNLLMGRELQKHYGQPAQCV
                     LTMPLLEGLDGVNKMSKSLGNYIGITDAPSDMFGKLMSISDTLMWRYLELLSFESLAT
                     IAKWREDVGGGRNPRDIKVLFAQEIVTRFHSRKAAEDALAEFEARFQKGVLPDDMPEV
                     SVLAPGGSIAVPQLLKQAGLVDSTSEAMRMIQQGAVKLDGERVSDKGATVASGSVVVA
                     QVGKRKFARISVGS"
     gene            1516..2811
                     /locus_tag="VFW59_10515"
     CDS             1516..2811
                     /locus_tag="VFW59_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5364689.1"
                     /translation="MPKEQTPAHKMKLRWFVTLSTMPLLGVVTAFGLVPESQLGLDAG
                     KLAIEDIALPKVAPLAVSATSFWRNEHVQRGDTVAELLRRLNVSDSNASDYLRTAVEA
                     ESFRKLPVGKEVQAETDASGGLISLRYMGEGDAQIVIEKKKDGYTSHTQPAQIEKRLF
                     VRTGEIKTSLYAATDAAGVPEGAANQLTEIFSGDVDFHHDLRRGDKFTVVYEMTYSNG
                     ALISAGRIQAAEFVNQGESYRAIYFQKDGQPQGDYFTPEGKSVRKAFLRSPIAFSRVS
                     SGFSNSRFHPVLNQWRAHKGVDFAAPIGTPVKVTSDGIVSLVGVQNGYGNVIMINHQG
                     HFSTVYGHLSRYAKGLHRGQRVAQGDVIGYVGMTGMTSGPHLHYEFKVDGTQRDPLRV
                     ALPNATPVNPADMEAFQGLAKNFVARINQLRNTNLAKLD"
     gene            2818..3915
                     /locus_tag="VFW59_10520"
     CDS             2818..3915
                     /locus_tag="VFW59_10520"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX5364690.1"
                     /translation="MREPELYIGLMSGTSLDGVDAVLADLGGEQTELIARHFLPFDDE
                     LKEELLALHHPSQNELHRAQLVGNQLARLYALSISPLLAQTGIPASKIHAIGCHGQTI
                     RHCPEHGYTLQIGNAALLAELTGIRVISDFRSRDIAAGGQGAPLVPAFHDKVLRHPTT
                     HRVVVNIGGISNLTNLPPNAATSGFDCGPGGLLMDAWCMRHLGQPYDRDGAWAASGRI
                     LPALLEQMLDETFFKLAPPKSSGRDLFNMGWLKTLLQGNEKAEDVQATLLELTCQSIV
                     QSVRQHCPGAQELYLCGGGAHNLALFSRLASLLEDCKVQTTSDLGVDSDYLEAIAFAW
                     LAQQTLHGKTANLPLVTGARHPCVLGAICPA"
     gene            complement(3985..4335)
                     /gene="erpA"
                     /locus_tag="VFW59_10525"
     CDS             complement(3985..4335)
                     /gene="erpA"
                     /locus_tag="VFW59_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX5364691.1"
                     /translation="MNAVTEMQDPLVFTDSAANKVKQLIEEEGNTDLKLRVFVSGGGC
                     SGFQYGFTFDEVANEDDTVMSKNGVQLLIDPMSFQYLVGAEIDYQEGLEGSQFVIKNP
                     NATTTCGCGSSFSA"
     gene            complement(4357..4767)
                     /locus_tag="VFW59_10530"
     CDS             complement(4357..4767)
                     /locus_tag="VFW59_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5364692.1"
                     /translation="MFNNKHSKPQSRIDTLIGEGTSIDGNLSFSGGMRIDGQVNGNVI
                     AVQDKPSTLVLSEHARVNGEVRVTHLVVNGTISGPVVASEYLELQGKARLAGDVHYKT
                     LEIQLGAIIEGRLIHSGTVAVQENVVALKLSVGE"
     gene            complement(4792..5478)
                     /locus_tag="VFW59_10535"
     CDS             complement(4792..5478)
                     /locus_tag="VFW59_10535"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEX5364693.1"
                     /translation="MRLHLPWYMRWSMMVPFLIGALAFAWWAYGSGLELAGFNRSQAY
                     GELSQLRARVALLDAENAKLKSQVVEYERQVQMEQASNQEAIKQMKSLSDESIALQED
                     LAFFQNLTAASSKEGELAVHRLRLERDKIPGEYRVRMLLVQGGQRAKEFNGSYQLVAT
                     VLENGQRSTRLFPQDASGYPQFRLGFKYYYRVEQNLLLPPEAQLESVQVRIFEQGTRE
                     PKLRQNVNLS"
     gene            complement(5534..6562)
                     /gene="argC"
                     /locus_tag="VFW59_10540"
     CDS             complement(5534..6562)
                     /gene="argC"
                     /locus_tag="VFW59_10540"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX5364694.1"
                     /translation="MIKVGIVGGTGYTGVELLRLLAAHPQVALQVITSRADAGTLVSQ
                     MFPSLRGYVDLPFTHPDEARLEQCDLVFFATPNGIAMQQVRALLDAGVKVIDLAADFR
                     LQDIPTWEKWYGMAHACPDLVAEAVYGLPEVNREKIKTARLIANPGCYPTAVQLGFIP
                     LLEAGLIETGSLIADAKSGVSGAGRKAEIATLFAEAADNFKAYGVAGHRHLPEIKQGL
                     ARVVGKDVGLTFVPHLTPMIRGIHATLYGRLTREIDLQALFEQRYADEPFVDVMPAGS
                     HPETRSVRGANRCRIAVHRPQGGDTVVVLSVIDNLVKGAAGQAIHNMNIMFGLPETTA
                     LDNVPLLP"
     gene            complement(6649..7041)
                     /gene="rpsI"
                     /locus_tag="VFW59_10545"
     CDS             complement(6649..7041)
                     /gene="rpsI"
                     /locus_tag="VFW59_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5364695.1"
                     /translation="MSANYNYGTGRRKSAVARVFIKPGKGDIVVNDKPVDVFFSRETG
                     RMVVRQPLALTETLGKFDIMVNVTGGGESGQAGAVRHGITRALIDFNAEFKPALKAAG
                     LVTRDAREVERKKVGFRKARRKKQFSKR"
     gene            complement(7044..7481)
                     /gene="rplM"
                     /locus_tag="VFW59_10550"
     CDS             complement(7044..7481)
                     /gene="rplM"
                     /locus_tag="VFW59_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5364696.1"
                     /translation="MKTFSAKAQEVQHDWLLVDAADQVLGRLAAQIAARLRGKHKAIY
                     TPHVDTGDFVVVVNADKLRVTGNKAEAKLYHRHTGYPGGLYTTNFTKLQQRHPQRVLE
                     KAVKGMLPKGPLGYAMLRKMKVYAGADHPHTAQQPKPVNLLKA"
     gene            complement(7625..9013)
                     /locus_tag="VFW59_10555"
     CDS             complement(7625..9013)
                     /locus_tag="VFW59_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX5364697.1"
                     /translation="MPALAIALDLGTTAIKAGLLSAGGELAGVVARTAPAVIASEGRY
                     QSDALAYAAEAEAVLAECLAQTPAPPPLGLCCQRSSFLIWERATGRPVTPLISWQDDR
                     GACEGLRGEEAEIRSLAGLPLTPYYLAPKLRRLLQENPSWRERLVRGDWLLGTLDTFL
                     IWRWSDGKHYVTDVSMAARTLLMDVRTRQWSPRLCELFGVPCGVLPEIRASVGWALPL
                     ERVGLTLRASVGDQSAAFVASIAAGQPEALVNLGTGGFVMRSLPEWLDVPEGYLRTLV
                     WQGGDMQAHFACEGTLNSIASVLAPYDVASCDADDLARDRIFCLAEPSGLGAPYFCKD
                     IGLTFSSPIEGLPSQRVAVLLLEGIIFRVARMLEEMRRECAVGRVYLSGGLSNVPCLQ
                     QGIARCAPFATTFRLHQSDAGLVGAARLSAGEPLIDGRRAEKVDILRPDSALLEKYGR
                     WKIWLDELLCRQ"
     gene            complement(9001..10539)
                     /locus_tag="VFW59_10560"
     CDS             complement(9001..10539)
                     /locus_tag="VFW59_10560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX5364698.1"
                     /translation="MRREFGTLQGSFDLLVCGGGIYGAWTAYDAALRGLKVALVEQGD
                     WAGATSSASSKLIHGGLRYLETYDFKLVRKALAERQRLLRIAPHRVWPLRFGVPVYAD
                     SRNGLLKLKAGLTLYDMLAGFPGEQMRHQYLNPAEFSGHFSYLDDATLRGGYTYGDAQ
                     TDDARLVLELVAGAMAHGAVCVNYARLVAWDEEGGRVCGATVCDELGGATVQVRARQC
                     VSTAGQWLVATAQGRIGCRLSKGVHLVLPGLASNEAILLTAKSDGRVFFIIPWYGRTL
                     LGTTDTDYRGEPDSVAVEASDVDYLLAAANGYLKTAWTKRDVIGSYAGLRVMKPSDDA
                     SPSDASRDWALKTTSNGLHHSVGGKITSAREDACSIVDAVCARLGSDAACTTGDRDLP
                     WKPEREDEDVALRCGIDAESALWLVRRHGNRSARVYALAAARPELAQRIVSDLPFILA
                     DLLFCAEDEMTVHLSDLLRRRMPLLILGRPGEGLLRRLAELVAPVLGWDAERVVQEVA
                     ACRR"
     gene            complement(10536..11288)
                     /locus_tag="VFW59_10565"
     CDS             complement(10536..11288)
                     /locus_tag="VFW59_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5364699.1"
                     /translation="MNPAHASMIFAHRGANREAAENTRGAFDRALSYPIDGIETDIQL
                     TRDGVAVLWHDRFLDKLGLPDKRIDDFDHGELLQMDFAAHFGDAGMREGVMSLREFLD
                     AYRTRCRLLLEIKNREWEPSERHESKVRKTLALVGAASDERILVSSFNLDSLVYAHQC
                     APDAALVLNLEPEHNAAFARAVLDVHPFLYGLCVHVATLNEEMVGALRERGKRVAVYT
                     CNSDEQIGRALGLGVDILISDVPQQALQMRDR"
     gene            11562..11957
                     /locus_tag="VFW59_10570"
     CDS             11562..11957
                     /locus_tag="VFW59_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5364700.1"
                     /translation="MSALQNSYASHIDDSNRKSPPLVAGGLSLSDDTGRVMASGISIL
                     LRPIEFRLLRFLMSHPERVHTRAELLEQAWGRKVFVGERTIDVHIRRLRVSLQPFGMD
                     RWIRTVHTRGYSFSTQYQATNINSLKETS"
     gene            11954..12961
                     /locus_tag="VFW59_10575"
     CDS             11954..12961
                     /locus_tag="VFW59_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5364701.1"
                     /translation="MKLSNITATLFVGALALSASGFAAEIKLLNVSYDPTRELYQDYN
                     AAFAKYWKARTGNDVVVKQSHGGSGKQARAIIDGLEADVATLALSGDIDALASKAWLI
                     PDNWQTRLKHNSSPYTSTIVFLVRKGNPKGIKDWDDLVKPGVSVITPNPKTSGGARWN
                     YLAAYGYALKHNNNNAAQGRAFLGKLFANVPVLDSGARGSTTTFVERGIGDVLLAWEN
                     EAFLAVKELGPDKFDIIVPSLSILAEPPVTVVDKNVDKHGTRKVAEAYLEYLYSPEAQ
                     EIVASNYYRPIDTKVAAKYAKQFPRLNLIKIDDAFGGWAKVQKEHFSDGGVFDQIYGK
                     K"
     gene            12988..13836
                     /locus_tag="VFW59_10580"
     CDS             12988..13836
                     /locus_tag="VFW59_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5364702.1"
                     /translation="MTHAFDLADLKKLLRNNLFLDLDSHALSETAHGLVCDFLDIRLS
                     SAFQPVIDAASNKVLGREALLRASAHDVADCTANAAFERAEEARSLVQFDRLLRTLHL
                     LNHACSFAADELLFLNVHPRLLVRVNDHGRTFEQILHYYSVPPERIVIEISENSVDDK
                     RRLFDAVNNFRNVGYKVAIDDFLPGRASLERVLELKPDIVKLDGRLIRDAEHVVSGKG
                     VVYGLVDILRSKGAGVVIQGIETAEQADIALRSGADGLQGYYLGQPAFPANTDERLGQ
                     GEPLAA"
     gene            14040..>14393
                     /locus_tag="VFW59_10585"
     CDS             14040..>14393
                     /locus_tag="VFW59_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX5364703.1"
                     /translation="MSSFRAHSVLPGFNLALGFTLFYLSLIVLIPLSALFLKTAELGW
                     GGFWDVVTGDRVVASLRVTFLTSFAAAIINVFFGLIVAWVLVRYRFPGKRIVDALVDL
                     PFALPTAVAGITLAML"
BASE COUNT         3039 a         4683 c         3878 g         2793 t
ORIGIN      
        1 catggcctaa ccatgaagat ttccttgtag attctttccc gctcagggtc gccagactat
       61 cacgagccga ccgaaatccg cgcgaacttg cgcttcccta cctgcgccac gaccacgcta
      121 ccagaggcga ctgtagcccc cttgtcgctg acacgctcgc cgtccagttt gaccgcgccc
      181 tgctggatca tgcgcatcgc ctccgaggtg ctgtccacca gcccggcctg cttgagcaac
      241 tgcggcaccg caatgctgcc gccgggcgct aaaacgctga cttccggcat gtcgtccggc
      301 aacacgccct tctggaagcg cgcctcgaat tccgccaagg cgtcttcggc ggccttgcgc
      361 gagtggaagc gcgtcacgat ctcctgcgcg aacaacacct tgatatcgcg cgggttgcgc
      421 ccccccccga cgtcttcgcg ccatttggcg atggtcgcca gcgactcgaa cgacaacaac
      481 tccagatagc gccacatcag cgtatcggaa atcgacatca gcttgccgaa catgtccgat
      541 ggcgcatcgg tgatgccgat gtaattgccc agtgacttgg acatcttgtt cacgccgtcc
      601 agcccttcca gcaacggcat ggtgagtacg cactgcgcgg gctgcccgta atgcttctgc
      661 agctcgcgcc ccatcagcaa gttgaacttc tgatcggtgc cgcccagttc gatatcggcc
      721 ttgagcgcca cggaatcgta accctgtacc agcggataga tgaattcatg gatcgcgata
      781 ggctgatttc ccttgtaacg cttggagaaa tcatcgcgct ccagcatctg cgccacggtg
      841 tgagtcgccg ccaaccggat aaggtcgacc gcgctgaatt tatccatcca ggtggaattg
      901 aacacgacct cggtcttggc cggatcgagc accttgaaca cctgatcgcg gtaggactgg
      961 gcgttttcca gcacctgctc gcgcgtcaac ggcgggcggg tggcattttt gccggtcggg
     1021 tcgccgatca tgccggtgaa gtcgccgatc agaaacagcg cgtggtgccc cagatcctgc
     1081 aactgacgca tcttgttcag caggacggta tgccccagat gcaaatccgg cgccgtggga
     1141 tcaaagcctg ccttgatgcg caacggcctg cccagtgcca gtttttgttt cagctcgccc
     1201 tcgagcagca actcgtgcgc cccgcgcttg atagtatcca gcgcgctggc gaaaggctcc
     1261 atgtgtttgg tttcagtcat aatttattgc caattcaagt tcaaacgaat accgctcgtt
     1321 agcattttca taccgtttat cgggcagagc ctgccgctcg actttttcga cctccatcag
     1381 tcagttctgt tttgagcgga agccggtttc tgttaaagtg cgcaccagtc agaaaaacaa
     1441 gacgttaccg ataaaacgga gcggcatcag gaggcgcgaa tgttaaccca aaaccggcac
     1501 caccaagaac gcaaaatgcc aaaagaacag acacccgcgc ataaaatgaa attgcgctgg
     1561 ttcgttacgc tgtccaccat gccactgctg ggtgtcgtga ctgcgtttgg cctggttccg
     1621 gaaagtcagc tgggcttgga cgcgggcaag ctcgccatcg aggacatcgc acttcccaag
     1681 gtcgcccctc ttgcagtcag cgcaaccagc ttctggcgca acgaacacgt acaacgcggc
     1741 gacaccgtcg ccgaattgct gcgccgcctg aacgtctcgg acagcaatgc cagcgactac
     1801 ctgcgcaccg ctgtcgaggc cgaatctttc cgcaagctcc ccgtcggcaa ggaagtgcag
     1861 gctgagaccg atgcctccgg cggactgatc tccctgcgtt atatgggaga gggcgatgct
     1921 cagatcgtga tcgagaaaaa aaaggacggt tacaccagcc acacccaacc cgcccagatc
     1981 gaaaaacgcc tgttcgtacg caccggcgaa atcaagacca gcctgtatgc cgcgaccgac
     2041 gctgcgggcg tgcccgaggg cgcagccaac cagctgaccg agatcttcag cggcgacgtt
     2101 gacttccacc acgatctgcg gcgcggcgac aaattcaccg tggtctacga aatgacctac
     2161 agcaacggcg cactgatcag cgctggccgc atccaggcgg ccgaattcgt caatcagggt
     2221 gaaagctacc gtgcgatcta cttccagaag gatgggcagc cacaaggcga ttacttcaca
     2281 ccggaaggca agagcgtacg caaggcattc ctgcgttccc ctatcgcctt ttcacgcgtt
     2341 agctcgggct tcagcaattc gcgtttccac ccggtactga accaatggcg cgcacacaag
     2401 ggcgtggact tcgccgcccc catcggcacc ccggtcaaag tcacctcgga tggcatcgtg
     2461 tctcttgtcg gcgtccagaa cggctacggc aatgtcatca tgatcaacca ccagggtcat
     2521 ttctccaccg tatacgggca cctctcgcgc tatgccaaag ggctgcaccg tggccagcgc
     2581 gtcgctcagg gtgacgtgat cggttatgtc ggcatgaccg gcatgaccag cggcccgcat
     2641 ctgcattacg aattcaaggt cgatggcaca caacgtgacc cattgcgcgt tgcactgccg
     2701 aatgccacgc cggtcaatcc agcagacatg gaagcatttc agggcctggc gaaaaacttc
     2761 gtcgcccgca tcaaccaact gcgcaacacc aacctggcca aactcgacta acgggccgtg
     2821 cgcgagcctg aactctacat tggcttaatg tcgggaacca gcctggacgg cgtcgatgcc
     2881 gttctggcgg atctgggcgg cgaacaaacc gaactgattg cccggcactt ccttccgttc
     2941 gatgacgaac tgaaggaaga gttgctggca ttgcaccatc ccagccagaa cgagttgcac
     3001 cgcgcccagt tggttggcaa tcagcttgcc cgactttatg ccctgagcat ctcgccgctc
     3061 ttggcccaga cgggtattcc cgcctcaaaa atccacgcca tcggctgcca cggacagact
     3121 attcgtcact gcccggaaca tggttacacc ctgcagattg gcaacgcggc tttgcttgcc
     3181 gaactgaccg gcatccgcgt gatcagcgac tttcgcagcc gcgacatcgc ggctggcgga
     3241 cagggagccc cgctggttcc ggcatttcac gacaaggtat tgcgccaccc gaccactcac
     3301 cgggtcgtgg tcaatatcgg cggcatcagc aatctgacca atctgcctcc caatgccgcc
     3361 acctccggct tcgattgcgg gcccggcggc ctgctgatgg acgcttggtg catgcgtcat
     3421 ctcggccaac cttatgaccg ggatggcgca tgggccgcat cgggccgaat cctgcccgca
     3481 ctgctggaac agatgctcga tgagacgttc ttcaagcttg ccccgcccaa aagcagcggg
     3541 cgcgacttgt tcaatatggg atggctaaaa acgctgctgc aagggaatga aaaagccgaa
     3601 gatgtacagg cgaccctgct ggaattgact tgccagagca tcgttcagtc tgtccggcaa
     3661 cattgccccg gcgctcagga actctatctg tgcggcggcg gagcccacaa tctcgcctta
     3721 ttttcgcgcc ttgcgtcact actggaagat tgcaaggtac agaccaccag cgacctcggc
     3781 gtggatagcg actatctgga ggcgatcgcc ttcgcctggc tggcgcaaca gaccctgcat
     3841 ggaaaaaccg ctaacctgcc gctggtcact ggcgcgcgac atccttgcgt gctgggagcc
     3901 atttgcccgg cttgacgaaa atctcaacgg ggctccaatg caaacggggc gcctgaagcg
     3961 ccccgttttt tattgaccca atgactatgc cgagaaagac gaaccgcaac cacaggtcgt
     4021 cgtggcattc ggatttttga tcacgaactg cgaaccttcc aaaccttcct gatagtcgat
     4081 ctccgcgccc accagatact ggaagctcat aggatcgatc agcagctgta cgccattctt
     4141 gctcatcacc gtatcgtctt cgttagcgac ctcatcgaag gtgaaaccgt actgaaaacc
     4201 ggaacacccc ccaccgctga cgaatacacg caacttgagg tcggtattgc cttcttcctc
     4261 gatcagttgt tttaccttgt tggccgcgct atcggtaaag accagcgggt cttgcatttc
     4321 agttactgcg ttcatgttgc tctcctaaaa tcctagttat tctccgacgc tcaatttgag
     4381 cgccaccacg ttttcctgta cggcaacggt accgctatgt atcaggcgcc cctcgatgat
     4441 cgcacccagc tgtatctcca gcgtcttgta gtgcacatca cccgcgagcc gggcttttcc
     4501 ctgcaactcc agatattcgc tggctaccac cggcccactg atcgtgccat tcaccacaag
     4561 atgcgtcacc cgcacctcgc cgttcaccct tgcatgctcg ctcagcacca gagtgctcgg
     4621 cttatcctgc acggcaataa cgttaccatt cacctgcccg tcgatgcgca taccgccact
     4681 gaaactcaaa ttgccgtcga tgcttgttcc ttcgccgatc aaggtatcga tacggctctg
     4741 cggtttactg tgtttattgt tgaacatcac tctcctcctg acctgacggc ctcaagatag
     4801 attcacgttc tgccgcaact tgggctcacg cgttccctgt tcgaatatac gtacctgaac
     4861 actctccagt tgcgcttccg gcggcaacaa aagattctgc tccacccggt aataatactt
     4921 gaaaccgagc ctgaattggg gatacccgga cgcatcctgt gggaatagcc gcgtactgcg
     4981 ctgaccgttt tcaagcaccg tggcaaccag ctggtaactg ccattaaatt ccttggcgcg
     5041 ctgtccaccc tgcaccagca acatccgcac gcgatattcg cccgggattt tgtcgcgttc
     5101 caagcgcagc ctgtgcaccg ccagctcccc ctccttactg ctcgcggccg tgagattctg
     5161 gaaaaatgcc aaatcttcct gcaaggcgat gctctcgtcc gacaggctct tcatttgttt
     5221 gatcgcctcc tgattgctgg cctgctccat ctgaacctgc cgctcgtact ccaccacctg
     5281 gcttttcaat ttggcattct ctgcatccaa cagagcaacc cgggcgcgca actgggacaa
     5341 ttcgccataa gcctgactgc ggttaaaacc cgccagctcc aatccactgc catatgccca
     5401 ccacgcaaag gcaagagcac cgatcaggaa cggaaccatc atgctccaac gcatatacca
     5461 aggcagatgc agccgcaccg acaatcgagg ggccgcgata ccgaacctgc gtttaacgcg
     5521 acgccaaatc atcctagggc aacagaggga cgttatcgag agcggtggtc tcgggcagac
     5581 caaacatgat attcatgtta tgaatcgcct gccccgccgc ccccttgacc agattgtcga
     5641 tcaccgacaa cacgacgacg gtgtcaccgc cctgcggccg atgcactgca atgcggcagc
     5701 gattcgcacc gcgcaccgag cgtgtttcag gatggctgcc agccggcatc acgtccacga
     5761 acggctcatc ggcatagcgc tgttcgaaca acgcttgcag gtcaatctcg cgggtcagcc
     5821 gcccataaag cgtggcatga atgccgcgta tcatcggcgt gagatgaggc acaaaagtca
     5881 gaccgacatc cttgccaacg acgcgcgcca gcccctgctt gatctccggc aagtgacgat
     5941 gacctgccac gccataggcc ttgaagttgt ccgccgcctc ggcgaacagg gtcgcaattt
     6001 ccgccttccg ccccgcgccg gacactcccg acttcgcatc ggcgatcaag ctccccgttt
     6061 caatcaggcc ggcctcaagc agcggaatga aacctaactg caccgccgtc ggatagcagc
     6121 ctggattcgc gatcagccga gcagttttga tcttttcacg attcacttcc ggcaggccat
     6181 acaccgcctc ggcgaccaag tccggacagg catgcgccat gccgtaccat ttttcccagg
     6241 tcgggatatc ctgcaaacgg aagtctgcgg ccagatcgat cacctttaca ccggcgtcga
     6301 gcaatgcgcg aacctgttgc atcgcaatgc cgttcggcgt ggcgaagaac accagatcgc
     6361 actgctccag cctggcctcg tcgggatggg taaacggcag atcgacataa ccgcgcagac
     6421 tggggaacat ctggctgacc agcgtgccgg catcggcacg tgaagtgatc acctgcaacg
     6481 cgacctgcgg atgcgccgcc aagaggcgca gcaactccac cccggtataa cctgtcccgc
     6541 caactatgcc tactttgatc atttcaccct tcccgtaacg cccacatcat atcagcaaac
     6601 accaaagcaa aagccgcccg aaggcggctt tgcgatacat ccaatcgatt aacgcttgga
     6661 gaattgcttc ttgcggcgcg ccttgcggaa gccgactttc ttacgttcca cctcacgtgc
     6721 atcgcgagtc accaaaccgg cagccttcag agccggtttg aattcggcgt tgaaatcgat
     6781 cagcgcacga gtgatgccgt gacgaacggc accagcctgg ccagattcgc cgccgccagt
     6841 tacgttcacc atgatgtcga acttgcccag tgtctcggtc agagccagcg gttgacgcac
     6901 gaccatgcgg ccagtctcac gggagaagaa aacgtccacc ggcttatcgt tcacaacgat
     6961 gtcgcccttg cccggcttga taaacacacg agccaccgca ctcttgcgac gaccggttcc
     7021 gtagttgtaa ttcgcgctca tgattaagcc ttcaacagat taacgggttt tggctgctgc
     7081 gcggtatgcg gatgatccgc accggcataa accttcatct tgcgcagcat tgcgtagccc
     7141 aatgggccct ttggcaacat gcccttaaca gccttctcca gcacacgctg cggatgacgc
     7201 tgctgcagct tggtgaagtt ggttgtgtac agaccgccgg ggtaacccgt gtgacgatgg
     7261 tacaacttgg cctcagcctt gttgccagtc acgcgcagct tgtcagcgtt gaccacgaca
     7321 acaaagtcgc cagtatccac gtgcggtgtg taaatcgctt tatgcttgcc gcgcaaacgc
     7381 gcagcaattt gagcagccaa acgacccagc acttgatctg ctgcatctac cagaagccag
     7441 tcatgctgga cttcctgggc cttagcggaa aatgttttca tagccacaat tcctaaaaat
     7501 tcaataagta aacctcgaag ggcgcgcatt ctatgtcagc gccccacccc tgtcaaacaa
     7561 aaattagctt tgcccgccaa gcagttcctg cagcgctcga cggatcgccc cgcaacctcg
     7621 acagtcattg cctgcacagc aattcatcca gccaaatctt ccagcgccca tacttttcca
     7681 gcagcgccga atcggggcgg agtatatcaa ccttctcggc acgccgcccg tcgatgagag
     7741 gctcgcctgc cgacaacctg gccgcgccga ccagcccggc atcggactgg tgcagacgga
     7801 aagtggtcgc gaacggcgca caccgcgcga tcccctgctg caagcaaggc acgttggaca
     7861 gaccgccgga cagatagaca cgcccgaccg cgcattcccg ccgcatctct tccagcatgc
     7921 gcgccacccg gaagatgatg ccctccagca gcaacaccgc cacgcgttgc gacggcaagc
     7981 cctcgattgg cgaagagaac gtcagtccaa tatccttgca gaagtagggc gcacccagcc
     8041 cgctcggctc ggcgagacaa aagatacgat ccctcgccaa gtcatccgca tcgcaggagg
     8101 cgacatcgta aggcgccagc accgatgcga tggaattgag cgtcccctcg caggcaaaat
     8161 gcgcctgcat atcgccgcct tgccacacca gcgtgcgcag ataaccttcc ggcacatcaa
     8221 gccattcagg gagactgcgc atcacaaaac cgcccgttcc cagattgacc agcgcctccg
     8281 gctgaccggc cgcgatgctc gccacgaaag ccgccgactg atcgcccacg ctggcccgca
     8341 acgtcaggcc aaccctctcc agcggcaacg cccacccgac tgaagcgcgt atctccggca
     8401 acaccccgca cggcacgccg aacaattcac acaagcgcgg cgaccattgc cgagtccgca
     8461 catccatcaa cagggtgcgc gcggccatcg acacatccgt cacatagtgc ttgccgtcac
     8521 tccagcgcca gatcaggaaa gtatccagcg tgcccagcaa ccagtcgccg cgaaccagcc
     8581 gctcgcgcca ggacggattt tcctgcaaca gccttcgcag cttgggcgcg aggtaataag
     8641 gcgtcagcgg caacccggca agcgagcgta tctcagcctc ctcgccgcgc agcccctcgc
     8701 acgcaccgcg atcatcctgc caggagatca gcggcgtgac gggtcgccca gtggcgcgct
     8761 cccatataag gaaagatgaa cgctggcagc acaatccaag cggcggaggc gccggcgtct
     8821 gcgccagaca ttcggccagc accgcctcgg cctccgccgc ataggccaac gcatcgctct
     8881 ggtagcggcc ttcgctagca atcacggcag gcgcggtgcg cgccacgacc ccggccagtt
     8941 cgccgcccgc agacagcaat cccgccttga tcgcggtcgt acccagatcg agcgcgatgg
     9001 ctagcgccgg catgccgcga cctcctgcac gacccgctcc gcatcccaac ccaacacggg
     9061 cgcgaccagt tcggccaagc ggcgcagcag cccctcgccc ggacgcccca gtatcagcaa
     9121 cggcatccgt cgccgcagca ggtcggacag atgcaccgtc atctcgtctt cggcgcagaa
     9181 caacaagtcg gccagaatga acggcagatc ggacacgatg cgttgcgcca gctccggcct
     9241 tgctgcggcc agcgcataaa cccgcgcact gcgattgcca tggcggcgca ccagccacag
     9301 cgcgctctcg gcatcgatgc cgcaacgcaa cgcgacatcc tcatcctccc gctccggttt
     9361 ccagggcaga tcgcgatcgc ccgtcgtaca cgcggcatcg ctgccgagcc ttgcacacac
     9421 cgcatcgacg atgctgcagg catcctcgcg cgccgaggtg atcttgccgc cgacggagtg
     9481 atgcaagccg ttgctcgtcg tcttcagcgc ccaatcgcga ctggcatcgg aaggcgacgc
     9541 gtcatcgctc ggcttcatca cccgcagccc ggcatagctg ccgatcacat cccgcttcgt
     9601 ccatgcggtc ttcaggtaac cgttcgccgc cgccagcaga taatccacgt cggacgcttc
     9661 gacggccacg ctatcgggtt cgccgcgata gtcggtatcg gtcgtgccca gcagcgtgcg
     9721 gccataccag gggatgatga agaacacgcg cccgtccgac ttcgccgtca gcaggatcgc
     9781 ctcgttcgat gccaagcccg gcaacaccag atgcacgccc ttgctcaggc gacacccgat
     9841 gcgaccttgc gccgtcgcga ccaaccattg accggcggtg ctgacacact gacgtgcgcg
     9901 aacctgcacc gtggcaccgc cgagctcgtc gcacaccgtc gcaccgcaca cccgcccgcc
     9961 ttcttcgtcc cacgccacga gtctggcgta attcacacac accgcaccgt gcgccatcgc
    10021 gcccgcgacc agctccagca ccagccgcgc atcgtcggtc tgcgcatcgc cgtaggtata
    10081 accgccgcgc agggtcgcgt cgtcgagata agagaagtgt ccggagaatt ccgcaggatt
    10141 cagatactga tgccgcatct gctcacccgg aaagcccgcg agcatgtcgt aaagcgtcaa
    10201 tcccgccttc agcttcagca gcccgttgcg gctgtcggca tacaccggca cgccgaagcg
    10261 caacggccac acccggtgcg gcgcgatgcg caacagacgc tggcgctccg cgagcgcctt
    10321 acgcaccagt ttgaaatcat aggtctccag ataacgcagg ccgccgtgga tcagcttgct
    10381 cgatgcagaa gaagtcgcac ccgcccagtc gccctgctcg accagcgcaa ccttcagccc
    10441 cctcagcgcg gcgtcgtatg ccgtccacgc gccataaatg ccgccgccgc agaccagcag
    10501 atcgaacgat ccctgcaatg ttccgaattc gcgtctcatc tgtccctcat ctgcaacgcc
    10561 tgctgcggca catcgctgat caggatgtcc acccccagac cgagcgcgcg gccgatctgt
    10621 tcatcgctgt tgcaggtgta gaccgcgacg cgtttgccgc gctcgcgcaa cgcgccgacc
    10681 atctcttcgt tcagcgtagc gacatgtacg cacagaccat acagaaaggg atggacatcc
    10741 agcaccgcgc gtgcaaaggc cgcgttatgc tccggttcaa ggttcagcac cagcgcagcg
    10801 tccggcgcgc attgatgggc gtagaccaga ctatccagat tgaatgacga caccagaatg
    10861 cgctcatcgc tcgccgcacc gaccagcgcc agcgtcttcc tgaccttgct ctcgtggcgt
    10921 tcgcttggct cccactcgcg attcttgatc tccagcagca ggcggcagcg cgtgcggtag
    10981 gcatcgagga attcgcgcag gctcatcacg ccttcgcgca tccctgcatc gccgaagtgc
    11041 gcggcaaaat ccatctgcag cagttcgccg tgatcgaagt cgtcgatgcg tttgtccggc
    11101 aagccgagtt tgtcgagaaa acggtcatgc cacagtactg cgacgccgtc tcgcgtgagc
    11161 tggatgtcgg tctcgatgcc gtcgatggga taggacagcg cccggtcgaa tgccccgcgc
    11221 gtattctccg ccgcctcccg gttcgcaccg cgatgggcaa atatcatgga tgcatgggca
    11281 ggattcatga acaagtcgag ctaaaccgga acgcgcacct tagcagggat gccggcaacg
    11341 taaaaggagc ttgatcgatt cgagctcaag cttgcgctat cgcccctgca tcgcgaccgg
    11401 ttagccggac aaggcgatcg atccagacag cgtccgttcg catattccaa aaacagctaa
    11461 tcaatggaaa tctaattggt tcacctgccg cacgaacctt cttacgatgc gctccatcgt
    11521 gaatgcactg ctcacgatca atctgaaaag gagcgcatgc aatgtcagcc ttgcaaaaca
    11581 gctatgccag ccatatcgac gactcaaacc ggaagagccc gccgctcgtt gctggcggac
    11641 tcagcctgtc tgacgacacc ggtcgtgtaa tggcatcggg catttcgatt ctgctgcgcc
    11701 cgatcgagtt ccggctgttg cgcttcttga tgagtcatcc ggagcgggtg catacacggg
    11761 cggagctgct ggagcaggca tggggcagga aagtattcgt cggcgaacgc acgatcgatg
    11821 tgcatatccg ccgattgcgg gtctcgctgc aacctttcgg catggatcgc tggatcagaa
    11881 cagtccatac ccgaggctac agtttctcga cgcagtatca ggcaaccaac atcaattcat
    11941 taaaggagac atcatgaaat tatcgaatat caccgcaaca ctgttcgtgg gcgcgctggc
    12001 actgtcggca tccggatttg ccgccgaaat caagctgctc aacgtgtcct acgatccgac
    12061 gcgcgagctt tatcaggact acaacgccgc gtttgccaaa tactggaaag ccaggaccgg
    12121 caacgacgtg gtggtcaagc aatcgcacgg cggttcgggc aagcaggcgc gcgccatcat
    12181 cgacggtctg gaggccgatg tggcgacgct ggcgctgtcc ggcgacatcg atgcgctggc
    12241 ctccaaggca tggctgatcc cggataactg gcagacccgc ctgaagcaca acagctcgcc
    12301 ttatacctcg accatcgtgt ttctggtgcg caagggcaac cccaagggca tcaaggactg
    12361 ggacgatctg gtcaagcccg gcgtgagcgt catcacaccg aacccgaaga cttccggcgg
    12421 cgcgcgctgg aactatctgg cggcctatgg ctacgcgctg aagcacaaca acaataatgc
    12481 ggcccaaggc agggcattcc tcggcaagct gttcgccaac gtgcccgtgc tggattcggg
    12541 cgctcgcgga tcgacaacca cctttgtcga gcgcggtatc ggcgacgtgc tgctggcctg
    12601 ggaaaacgaa gccttcctcg ccgtgaagga actggggccg gacaagttcg acatcatcgt
    12661 gccgtccttg agcattctgg ccgagccgcc ggtgaccgtg gtggacaaga atgtggacaa
    12721 gcacggcacg cgcaaggtgg ccgaggccta tctggagtac ctgtattcac cggaagctca
    12781 ggagatcgtt gccagcaatt actaccgccc gatcgacact aaggtggcgg ccaagtatgc
    12841 gaagcagttc cccaggctga atctgatcaa gatcgacgac gcattcggcg gctgggccaa
    12901 ggtgcagaaa gaacacttct ccgatggcgg cgtgttcgat cagatctacg gcaagaagta
    12961 agcatcgcat gaacaggaga cctaaaaatg acacatgcct ttgatcttgc cgacctgaaa
    13021 aaactgctgc gcaacaatct gtttctcgac ctggactctc atgccctgag cgaaacggca
    13081 catggtctgg tgtgcgactt tttggacatc cgcctgagca gtgcctttca gcctgtcatc
    13141 gatgcggcca gcaacaaggt gctggggcga gaagccctgt tgcgcgcctc tgcccatgat
    13201 gtcgcagact gcaccgcaaa cgcagcattc gagcgagccg aggaggcgcg cagtctggtg
    13261 cagttcgacc ggctgctgcg caccctgcac ctgctgaacc atgcatgcag cttcgccgcc
    13321 gacgagttgt tgttcctgaa cgtgcatccg cgcctgctgg ttcgggtcaa cgatcacggc
    13381 cgcaccttcg agcagatact gcattactac tcggtgccgc ccgaacgcat cgtcatcgag
    13441 atctccgaga attcggtgga tgacaaacgg cgtctgttcg atgcggtaaa caacttccgc
    13501 aacgtcggct ataaagtcgc aatagacgat ttcctgcccg gtcgcgccag tctggaacgg
    13561 gtgctggaat tgaagccgga catcgtcaag ctggatggcc gcctgattcg cgatgcagaa
    13621 catgtcgtaa gcggcaaggg cgtggtgtat ggcctggttg acatccttcg cagcaagggt
    13681 gccggggtgg tcattcaagg catcgaaacc gcagagcaag ccgacatcgc cctgcgttcc
    13741 ggagcggatg gattgcaggg ctattacctc ggtcaacccg cattcccggc caataccgat
    13801 gagcggcttg gtcagggcga gccgctagcc gcctgatcgt tctctctcct cacgggtgca
    13861 acatccggtt cacaggggca ggcgactgcc cctgtttttt cgtccataaa atccaaaatt
    13921 aactttttgt tatgaattat tctttattcc aaaaactgct aatcaattca gtttttatta
    13981 ttttcctgcg ggatgacgca tcgctagact gcgcgcttat tcaacaacag gaataagcaa
    14041 tgtcgtcatt tcgtgcgcac agcgtgctgc ccggtttcaa tctggcgctg ggttttacgc
    14101 tgttttatct ctcgctgatc gtgctgatcc cgttgtccgc gctgtttctc aagacggcgg
    14161 agctggggtg gggcggcttc tgggatgtcg tcaccggcga ccgcgtggtg gcttcgctgc
    14221 gcgtgacctt cctcacctct ttcgcggcgg caatcatcaa tgtgtttttc ggtttaatcg
    14281 tggcgtgggt gttggtgcgc taccgcttcc ccggcaagcg cattgtcgat gcgctggttg
    14341 atttgccgtt tgcgctgccg acggcggtgg cgggcatcac gctggcgatg ctg
//
LOCUS       DASUIV010000295         5283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1006842, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000295 DASUIV010000000
VERSION     DASUIV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006842"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VFW59_10590"
     CDS             <1..139
                     /locus_tag="VFW59_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX5364704.1"
                     /translation="KILQEVITLTIFVPFAVLYMNQPLKMDYLYAGLCLMGAVYFIFR
                     A"
     gene            230..1609
                     /gene="mpl"
                     /locus_tag="VFW59_10595"
     CDS             230..1609
                     /gene="mpl"
                     /locus_tag="VFW59_10595"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX5364705.1"
                     /translation="MTTPKHIHILGICGTFMGGIAAIAKQAGYRVTGCDANVYPPMST
                     QLEAQGIELIEGFGVEQLRLNADVYVIGNVVSRGNPLMEEILNRNLPYISGPQWLADT
                     LLRDKWVLGVAGTHGKTTTTSMLAWILEHAGLNPGFLIGGVPQNFGISARITESPFFV
                     IEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRSVPGN
                     GLVVCNGREDSLQRVLKRGCWTPVEMFGIPLPTPVPPAGEGAEMRGWHIDAKDLVTLD
                     GEPQGTLRWELMGEHNRMNALAALAAARHAGVPVAHGLEALAEFKNVKRRMEVRGTVN
                     GITVYDDFAHHPTAIDTTVAGLRHKVGTARILAVLEPRSNTMKLGVMKDALPGSLKDA
                     DRVFCYAGNLGWDARGALAPLGDKAVVKDDLDELVEAIAAVARPGDQVLVMSNGGFGG
                     IHEKLLKRL"
     gene            complement(1681..3081)
                     /gene="ntrC"
                     /locus_tag="VFW59_10600"
     CDS             complement(1681..3081)
                     /gene="ntrC"
                     /locus_tag="VFW59_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX5364706.1"
                     /translation="MKPVWIIDDDRSIRWVLEKSLTREDIEFKSFASADDAMRELKSG
                     QPQVVVSDIRMPGSSGLDFLQALRQRHPDIPVIIMTAYSDLESAVSAFQGGAFEYLPK
                     PFDINHAIELIRRALEQSTRKSGETEKVEVATEILGQAPAMQEVFRAIGRLSQSNATV
                     LINGESGTGKELVARALHRHSARADQAFIAINTAAIPKDLLESELFGHERGAFTGAAA
                     QRRGRFEQAEGGTLFLDEIGDMPSDLQTRLLRVLSDGNFYRVGGHQPVKANVRIITAT
                     HQDLEERVKQGLFREDLFHRLNVIRLRLPPLRERREDIPLLAKYFLQKSARELGVEQK
                     RVSEEALRFLATQDFPGNVRQLENLCHWLTVMTPSQVVEIADLPPEWRGSGSESAVNS
                     GDWSAALALQAAVALQRGDERIMDTLTAQFERTLILQALHHTGGRRVEAATLLGIGRN
                     TLTRKIQELGLENQEP"
     gene            complement(3091..4134)
                     /gene="glnL"
                     /locus_tag="VFW59_10605"
     CDS             complement(3091..4134)
                     /gene="glnL"
                     /locus_tag="VFW59_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX5364707.1"
                     /translation="MPNPITQLSLEYLATAVILLDEESRIAYLNPAAEHLFDVSGKNL
                     LGHALQHVFTETEQLANAIQQAVHNNASYIEHDLTLGTPTRGKLHLRCAVTPVQFAKR
                     QLLLEFHPMDRPLKLAREEQMLDQTQANRLLLRNLAHEIKNPLGGIRGAAQLLEQELD
                     RADLREYTQVIVQEADRLRSLMEKLLTPQNGSHYSALNIHEVLERVRSVVLAEMPEGL
                     KIRRDYDISLPALVGDKEQLIQTMLNIVRNAAQAMQGHGQIILRTRIARQVTLIKRRY
                     RLAVMVQIIDNGPGIPHELQDKIFYPLVSGRADGHGLGLTLAQDFISQHNGMIEFDSE
                     PGRTCFTVLLPLP"
     gene            complement(4168..4638)
                     /locus_tag="VFW59_10610"
     CDS             complement(4168..4638)
                     /locus_tag="VFW59_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5364708.1"
                     /translation="MKLQHLLVLLCAWPMLAQAEIYKSVDKDGHVTYSSSPIRGGKRI
                     ILEPNLSVPSERSRSTAAAENFPRVDGRTQRGRDDTRRQILDEELATEEKLLAEANQA
                     LKEGEAAPEVYRGKDGKTYRNVAKYDEKVKALKEQVDLHQRNVEALKTELSRLR"
     gene            complement(4760..>5283)
                     /gene="glnA"
                     /locus_tag="VFW59_10615"
     CDS             complement(4760..>5283)
                     /gene="glnA"
                     /locus_tag="VFW59_10615"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165543.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX5364709.1"
                     /translation="YYIGGIIKHAKSLNAICNPGTNSYKRLVPHYEAPVKMAYSAKNR
                     SASIRIPHVASDKARRIEARFPDPTANPYLCFSALMMAGLDGIQNKIHPGDPADKNLY
                     DLPPEEDAKVPTVCASLDEALDALAKDHEYLTRGGVFSKDFLDAFITLKMEEVNKIRM
                     TTHPVEFDMYYSI"
BASE COUNT         1032 a         1608 c         1547 g         1096 t
ORIGIN      
        1 gaagatattg caggaagtca tcacgctgac gatcttcgtg ccgttcgcgg tgctgtacat
       61 gaaccagccg ctgaagatgg attatctgta tgcggggctg tgcctgatgg gcgcggttta
      121 ttttattttt agggcgtaag gaaaactgtc cacgaaaaac acgaaaggca cgaactaaca
      181 aattggttcc agtctcttgt tcgtgttttt cgtggatgat taagaaacca tgaccactcc
      241 aaaacacatc cacatcctcg gcatctgcgg caccttcatg ggcggcattg cggctatcgc
      301 caagcaggcg ggctatcgcg tcaccggctg cgacgccaac gtctatccgc cgatgagcac
      361 gcagctggaa gcgcagggca tcgagctgat cgagggcttc ggcgtcgagc agttacgcct
      421 gaatgccgat gtttatgtga tcggcaacgt ggtgtcgcgc ggcaacccgc tgatggaaga
      481 aatcctcaac cgcaacctgc cctatatctc cggcccgcag tggcttgcgg atactttact
      541 acgagacaag tgggtgctgg gcgtagcggg tactcacggc aagaccacca cgacttcgat
      601 gctggcctgg atactggaac atgccggact gaatcccggc ttcctgatcg gcggcgtgcc
      661 gcagaatttc ggcatctcgg cacgcatcac cgagtcgccg ttcttcgtga tcgaagcgga
      721 cgaatacgac acggcatttt tcgacaagcg ctccaagttc gtacactacc gtccgcgcac
      781 cgcgatcctg aacaacctcg aattcgacca cgccgacatc ttccccgatc tcgccgcgat
      841 cgagacccag ttccatcatc tggtacgcag cgtgccgggc aacgggctgg tggtctgcaa
      901 tggccgcgag gactcgctgc aacgcgtgct caagcgcggt tgctggacgc cggtggagat
      961 gttcggcatt cctcttccca ctcccgtccc gccggcagga gagggcgccg agatgcgggg
     1021 ctggcacatc gacgcaaaag acctcgtgac gctggacggc gaaccgcaag gcaccctgcg
     1081 ctgggagctg atgggcgaac acaaccgcat gaatgcgctg gccgcactgg ctgccgcccg
     1141 tcatgccggc gtgccggttg cccacggtct ggaggcgctg gccgagttca agaacgtgaa
     1201 acggcgcatg gaagtgcgcg gcacggtcaa cggcatcacg gtatacgacg acttcgccca
     1261 tcaccccacc gcgatcgaca ccaccgttgc cggactgcgc cacaaggtcg gcacggcacg
     1321 catcctggcg gtgctggagc cgcgctccaa caccatgaaa ctgggtgtga tgaaggacgc
     1381 actgcccggc agcctgaagg atgccgaccg ggtgttctgc tacgcgggca atctgggctg
     1441 ggatgcgcgc ggtgcgctgg cgccgctggg cgacaaggcc gtggtgaaag acgacctcga
     1501 cgagctggtc gaggctatcg cagccgttgc ccgcccgggc gatcaggtgc tggtgatgag
     1561 caacggcggc ttcggcggga tacacgagaa gctgctgaaa cgcctgtagg ggcacggcgt
     1621 aacaaagtca ggcaatccac tatccagctt gctggatgtg gaattgacac cgccgtgccc
     1681 ctacggttcc tgattctcca gccccagctc ctgtatcttg cgcgtcagcg tgttgcggcc
     1741 gatgccgagc agcgttgccg cctcgacccg ccgcccgccg gtgtgatgca aagcctgcag
     1801 gatcagcgtg cgctcaaact gggcagtgag cgtgtccatg atgcgctcat cgccgcgctg
     1861 taaagccacc gccgcctgga gcgccagtgc cgcactccag tcaccgctgt taactgcact
     1921 ctcactgcct gagccccgcc attcgggagg aagatcggcg atctcgacga cctgagaagg
     1981 cgtcatcacc gtcaaccagt ggcacaggtt ctccagttgc cgcacattgc cgggaaagtc
     2041 ctgggtggcc aaaaagcgca aagcctcttc gctgacccgc ttctgctcca cacccagttc
     2101 gcgcgcactc ttctgcagga aatatttcgc cagcagcggg atgtcttcgc ggcgttcgcg
     2161 cagcggcggc aagcgcaaac ggatcacgtt cagccggtgg aacaggtctt ctcggaacag
     2221 cccttgtttg acgcgctcct ccagatcctg atgagtggcg gtgatgatgc gcacgttggc
     2281 cttgaccggc tggtgtccgc cgactcggta gaagttgccg tccgacagca cgcgcaacaa
     2341 gcgggtttgc aggtcggatg gcatgtcgcc gatctcgtcc agaaacaagg tgccgccctc
     2401 ggcctgttcg aaacgtccgc gacgctgggc ggccgcaccg gtaaaggccc cccgttcatg
     2461 gccgaacaat tcggactcca gcagatcctt cgggatcgcc gccgtgttaa tcgcgatgaa
     2521 ggcctgatcg gcccgcgcgc tatggcggtg cagcgcgcgc gccaccagct ccttgccggt
     2581 gcccgattca ccgttgatca gcaccgtggc attcgattgc gaaagacggc cgatcgcgcg
     2641 gaatacttcc tgcatcgcgg gcgcctgacc gagaatctcg gtggccactt caactttctc
     2701 cgtctcgccc gacttgcgcg tgctctgctc cagcgcgcga cggatcagct cgatggcatg
     2761 attgatgtcg aacggcttgg gcaggtattc gaacgcgccg ccctgaaatg ccgatacggc
     2821 actctccaga tcggagtagg cggtcatgat gatcaccgga atatcagggt ggcgctggcg
     2881 cagcgcctgc aggaaatcca gaccggaact gcccggcatg cggatgtcgc tgaccaccac
     2941 ctgcggctgt ccacttttta actcgcgcat cgcgtcgtcc gccgaggcaa aactcttgaa
     3001 ctcgatatct tcgcgcgtca gggatttttc cagcacccaa cggatggagc ggtcgtcgtc
     3061 tatgatccag actggtttca tgattgtcac ctatggtaag ggtagtagca cggtgaaaca
     3121 ggtgcggccg ggctcgctgt cgaactcgat catgccgtta tgctggctga tgaaatcctg
     3181 cgccagcgtc agccccaatc catgcccgtc ggcgcggccc gaaaccagcg gatagaagat
     3241 cttgtcctgt aactcgtgcg ggatgcccgg accgttatcg atgatctgca ccatcaccgc
     3301 cagccggtaa cgccgcttga tcaacgtcac ctggcgcgcg atgcgggtgc gcagaatgat
     3361 ttgcccgtga ccttgcatcg cctgagcggc attgcgcacg atattgagca tggtctgtat
     3421 caactgctcc ttgtctccga ccagtgcggg caggctgatg tcgtagtcgc ggcgtatctt
     3481 caggccctcc ggcatctctg ccagcaccac gctgcgcacc cgctccagca cttcatggat
     3541 attgagcgcg ctgtagtgcg acccgttctg cggcgtcagc agcttttcca tcagcgaacg
     3601 cagacgatcg gcctcctgca cgatgacttg ggtatattcg cgcaggtcgg cccgatccag
     3661 ttcctgctcc agcagctgtg ccgccccacg tatgccgccc agcggattct tgatctcgtg
     3721 cgccagattg cgcagcagca atcgattggc ctgcgtctga tccagcatct gctcttcgcg
     3781 cgccagcttc agcggtctat ccatcggatg aaattccagc agcagctggc gcttggcgaa
     3841 ttgaaccggc gtcaccgcgc aacgcagatg cagcttgccg cgcgtcggcg tgccgagcgt
     3901 caggtcgtgt tcgatatagc tcgcgttatt atgaactgcc tgctgaatag cgttcgccaa
     3961 ctgctcggtt tccgtgaaca catgctgtaa cgcgtgcccg agcaaattct tgccgctcac
     4021 atcgaacaga tgctctgcgg caggattgag ataggcgatg cgcgactctt catccagcag
     4081 gatgacggct gtggcgagat attcgagcga gagttgagta atgggattag gcatatcttt
     4141 ctgttaagca gaaaacatgc cagtgcatca tctgagtctg gacaactcgg tcttgagcgc
     4201 ctcgacattc ctctggtgca gatcgacttg ttctttcagc gccttcacct tctcgtcata
     4261 cttggcgaca ttgcgatagg ttttgccgtc cttgcctcga tacacttccg gcgccgcctc
     4321 gccttctttc agggcctgat tggcttctgc cagcagcttt tcctcggtcg ccagctcttc
     4381 atccagtatc tgacggcgag tatcgtcccg ccccctttga gttcggccat caaccctcgg
     4441 gaagttctcg gctgctgccg tcgagcggga acgctcagaa ggcaccgata aattcggctc
     4501 cagaataatc cgcttgcctc ccctgatcgg agagctggaa taggtgacat gcccgtcctt
     4561 atcgacagac ttgtatatct cggcctgcgc cagcataggc caggcgcaca atagaaccaa
     4621 caggtgttgc agtttcatgt tcgattcccg caaaggtaca tggcaagtat aggtcatgcg
     4681 tccagcacag acaaaccctt atggcgccca taaaaaaacg gggcgccagg ccccgttttt
     4741 tactgcatca acaaacctgt tagatgctgt agtacatgtc gaactcgacc gggtgagttg
     4801 tcatgcggat cttgttcact tcttccatct tcaaggtgat aaacgcatcg aggaagtcct
     4861 tggagaacac gccgccacgg gtcaggtact cgtgatcctt ggccagagcg tccagcgcct
     4921 catcgagcga tgcgcacacg gttgggactt ttgcgtcctc ttccggaggc agatcgtaca
     4981 ggttcttgtc agccggatcg ccggggtgaa tcttgttctg aatgccgtcc agacctgcca
     5041 tcatcagagc cgagaaacac aagtacgggt tcgcggttgg atcagggaag cgggcctcga
     5101 tacgacgagc cttgtcgctg gcaacgtgcg gaatgcggat cgaagcggaa cggttcttcg
     5161 ccgagtaggc catcttcacc ggcgcttcgt agtgaggaac cagacgcttg taggagttgg
     5221 tgccgggatt gcagatcgcg ttcagcgact tggcatgctt gatgataccg ccgatgtagt
     5281 aca
//
LOCUS       DASUIV010000296         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1379091, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000296 DASUIV010000000
VERSION     DASUIV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379091"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VFW59_10620"
     CDS             <1..688
                     /locus_tag="VFW59_10620"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX5364710.1"
                     /translation="LMLSTDHYLLGWSMALALFRMAFLVMLERTLVQFMKGAFQVGIL
                     QHPLLDGAIKLLGLLLVLGGLLPSVLTACAGLLLALLLMLRFAFWKPHLAMKRLDIGI
                     MYLGYLAIVAQLLVEFFRLILPAQELTSLSIHVFTFGAMGLIIPAMLIRISQGHTGRK
                     VAFDRRDGFVLRIMLLAFLLRIVAPQLYPASYTLWVALSAACWFASFVLLAWRYIPML
                     LSRRVDGKEY"
     gene            790..3579
                     /locus_tag="VFW59_10625"
     CDS             790..3579
                     /locus_tag="VFW59_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5364711.1"
                     /translation="MHREAFMLNGQKKSISIWSSLAHKFRNSIFAIFLPFSLLVVLAS
                     AYLFYQQLAHQKDAIKENGILDAISASHVIERSLAGSVRDILYLANDQELLAAAAKKN
                     HKQQDLQSLLKELSLFAALNPKVHRIRWVDDDGLDILNIKNDKGLVEVLGAAERDDIS
                     DRYYFKKTVGLAQGQVYVSPLDLEFVHGKLTEPYQPLVRISTPVFDRQGRRNGILVVA
                     IDAQDFLSHLKPNAMNPDNHLMLLNRDGYWLKGRNPEEEWGFMFNRARTFASEYSSAW
                     KEIAFADQGSFEDESGLWSFQTVYPLRSEQSAVAQAGETKLGEYYWKVVSFVPREKTV
                     KLHYEVMWAIGYPALGLLLLVFVGSWAYVRLRKSKQVTERDLGMLASALEINEAILIA
                     DADCNILRVNQAFSEVTGYSAEEVLGKNPSILKSDRQDETFYQGMWNRLLQDGYWEGE
                     IWDRRKNGEIYPALLKISTVPDHTGGVSYYVSTFSDISERKASEGRISFLSFYDPLTE
                     LPNRKYVMDRLAHIIRADHGADKYAAIISIDLDNFKKINEGLGFGEGDQLLVEATKRI
                     KSCIVRDDSAARVGVDEFFVLQEDLSGDGSEGASQVERIAQRIRASLSQPYLLKDNTC
                     KCTASIGVTLFRVADATLDEILRRVEVATFRAKQAGGDAVRFFDPVTQKALEKNLALE
                     LDLRSALGNKELLLCYQLQVDHHQQPVGAEVLLRWVHPRNGVILPDEIIPLAEEIGLM
                     QPISCWMFETVCQQIKSWEAYPILRDLVIAVNVSVRQFADAGFVEQLTGSITRSGINP
                     ARLQLELTEDSLVSDIDLVASKMRTIRGLGVKFALDDFGTGYSSLTYLKRLPLDQLKI
                     DKSFVRDVTTNKEDLAIVKTIISMSHTLGLNVIAEGVETQEQMSLLEQSGCMNFQGYL
                     FSRPLPVSEFEGLFA"
     gene            complement(3652..3909)
                     /locus_tag="VFW59_10630"
     CDS             complement(3652..3909)
                     /locus_tag="VFW59_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364712.1"
                     /translation="MTLALQAGFISPALLLEFMWQLPHRDDRFVRTGIVGAVQITKRF
                     KVRLELPSGTMFKLSAYQPATAPGARQEPPQLEFFEVTKMP"
     gene            complement(4019..>5377)
                     /locus_tag="VFW59_10635"
     CDS             complement(4019..>5377)
                     /locus_tag="VFW59_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5364713.1"
                     /translation="SRPVLKTVDSRVTGGAINGEGVLTVRTTAIGAETTLARIIRLVE
                     SAQAGKAPIQRLVDQVAAVFVPVVLLVAALTFAGWLLAGSDWEPALINAVSVLVIACP
                     CALGLATPTAVVVATGRAAHAGILVKDAAALERAHKLAVLVVDKTGTLTEGKPSVTDL
                     LPVDGVAQDQLLRVAAALAQHSGHPLSRALLDHAGQIDIPEVANLNEHAGQGLSAELD
                     GKPCLLGSPAYVTQQGVTLDESQLTALQQQGKTVIGVTRDGQLLGLIALADRLRPSSR
                     NAVARLHEMGVRVVMLSGDNLATAEAIAAQAGITEFRAEVLPQDKAALVESYKQAGKL
                     VGMAGDGINDAPALAAADVGFAMKSGSDIALEAADITLMRNDLASVVDAISLSRATLA
                     KIRQNLFFAFGYNVLGIPLAAFGMLNPMIAGAAMAMSSVSVVSNALLLKRWRSPLSRE
                     SR"
BASE COUNT         1071 a         1416 c         1616 g         1274 t
ORIGIN      
        1 tctgatgctg agcacggatc attacctgct cggctggagc atggcgctgg cactgttccg
       61 tatggccttt ctggtgatgc tggagcgcac gctggtgcag ttcatgaagg gcgcgttcca
      121 ggtcggcatc ctgcagcatc ccttgctcga tggggcgatc aaattgctcg gtctgctgct
      181 ggtgttgggc gggctgttgc cgtccgtcct gacggcctgt gccggactgc tgttggcgct
      241 gttgctgatg ctgcgcttcg cgttctggaa gccgcatctg gcgatgaagc gtctggacat
      301 cggcatcatg tatctcggtt atctggccat cgtcgcgcaa ctgctggtgg aattcttccg
      361 cttgattctc cctgcacagg agctcacctc gctgtccatc catgtgttca ccttcggtgc
      421 gatggggctg atcatcccgg ccatgctgat ccgcatctcg cagggacaca ccggcaggaa
      481 agtggcattc gaccgtcgcg atgggttcgt cttgcgcatc atgctgctgg ctttcttgct
      541 gcgcattgtc gctccgcagt tatatccggc cagctatacg ctgtgggtcg cactgtcggc
      601 ggcctgctgg tttgccagct tcgtgctgct cgcctggcgc tacattccga tgctgctcag
      661 tcggcgcgtc gatggcaaag aatattagct catgtgaata gtcccttgtt ttaaaatggt
      721 catgctgtta atgtttgaaa aatatgacat ttattgaata attattattc ggatatagat
      781 gattaacgca tgcacagaga agcctttatg ttgaatggac aaaaaaaatc aatttcgatc
      841 tggagctcgc tggcgcataa atttcgtaac agtatctttg caatcttcct gcccttttcg
      901 ttgttggtcg ttctggcatc tgcttatctg ttttatcaac aactcgccca tcaaaaggat
      961 gccatcaagg aaaacggcat actggatgcg atatcggcaa gccacgtcat tgagcgctcg
     1021 ttggccggga gcgttcggga tatcttgtat cttgcaaacg atcaggaatt attggctgcg
     1081 gcggcgaaga aaaatcacaa gcaacaggac ctgcaatctc tgctcaaaga actttccctg
     1141 ttcgccgcat tgaatccgaa ggtgcacagg attcgttggg tcgacgatga tggccttgat
     1201 atcctgaaca tcaagaacga taagggtctt gtcgaagttc tcggtgccgc tgaacgtgat
     1261 gatataagcg atcgttacta ttttaagaag acggttggat tggcgcaggg gcaggtatat
     1321 gtatcgcctc tcgatcttga gtttgtccat ggaaagctta ccgagcctta ccagccgctc
     1381 gtcaggattt ctacgccggt ttttgatcgt caaggtcgcc gaaatggcat attggtcgtg
     1441 gctatcgatg cgcaggactt tttgtcgcat ctcaaaccca acgcgatgaa cccggacaat
     1501 catctgatgt tgttgaaccg ggatggttat tggttgaagg gacgcaaccc cgaggaagag
     1561 tgggggttta tgttcaatcg cgccaggacg tttgcctccg aatattcctc ggcatggaaa
     1621 gagatagcct tcgccgatca aggcagcttt gaagatgaaa gcggattgtg gagctttcaa
     1681 accgtctatc cattgcggtc ggagcaaagt gctgtcgcgc aggctggcga aaccaagttg
     1741 ggcgaatact actggaaggt ggtgtcattc gttccgcgag agaagactgt caaactgcac
     1801 tatgaggtga tgtgggcgat tggctacccc gctttgggat tgctgctgct ggttttcgtc
     1861 ggttcctggg cttatgtgcg gttgcgcaag tcgaagcagg tgactgaacg cgatctgggg
     1921 atgctggcga gtgctctgga aatcaatgaa gccatcctga tcgcagatgc cgattgcaac
     1981 atcctccgtg ttaaccaggc attttccgaa gtgacgggat attctgcaga agaagtgctg
     2041 ggcaagaatc catcgatact caaatctgac cgacaagatg aaacctttta ccaggggatg
     2101 tggaataggc tgttgcagga tggctactgg gaaggcgaaa tatgggatcg ccgcaagaat
     2161 ggcgagatat accccgcgct gttgaaaata tcgactgtcc ccgatcatac cggcggggtg
     2221 tcttattacg tatcgacttt tagcgatatc tctgagcgca aagcctccga ggggagaatc
     2281 agcttcctgt ctttttatga tcccctgaca gaattgccca accgcaagta tgtgatggat
     2341 cggttggcgc acattattcg ggccgatcat ggcgccgata aatatgccgc gatcatttcc
     2401 atcgatttgg ataatttcaa gaagataaat gaaggtctgg gatttggtga aggtgaccag
     2461 ctgctggttg aggctaccaa acggatcaag tcatgtatcg ttcgggacga tagtgccgca
     2521 agagtcgggg tggacgaatt cttcgtgctg caggaagacc tgagcggtga tggttcagaa
     2581 ggcgcgagcc aggtcgaacg gatcgctcaa agaattcgtg cctccctgag tcagccttac
     2641 ctgttgaaag acaatacctg caaatgcacc gccagcattg gtgtgacttt gtttcgcgtg
     2701 gcggatgcaa cgctggatga aatactcagg cgggtcgagg tggcaacttt cagggcgaaa
     2761 caggccggag gcgatgcggt tcgatttttt gaccctgtga cacagaaagc tcttgaaaag
     2821 aatcttgctc tggagctcga cctccgatcg gcgctaggca ataaagaatt gctgctttgt
     2881 taccagttgc aggttgacca tcatcaacag cctgtcggtg ccgaggtgct gttgcgttgg
     2941 gttcatcccc gtaatggcgt gatattgccg gatgagatca tcccgcttgc cgaagagatc
     3001 gggctgatgc agcccatcag ctgctggatg tttgaaacgg tctgccaaca gatcaagagt
     3061 tgggaggcgt atcccatcct gcgtgatttg gtcatcgccg taaatgtcag cgtacgacaa
     3121 ttcgctgatg ccggatttgt cgagcagctc acaggcagca tcacgcgcag cggtatcaat
     3181 cctgcccgac tgcaactcga attaacggag gattcgttgg tgagcgatat cgatctggtg
     3241 gccagcaaga tgcggacgat ccgtggcttg ggggtcaagt ttgcgctgga tgatttcggt
     3301 acgggctact cttcgttgac ctatctcaag agattgcctc tggatcagct caagatcgat
     3361 aaatcctttg ttcgtgatgt aacgacaaac aaggaagatt tggcgattgt gaaaacgatc
     3421 atcagcatgt cccacaccct cggtttgaat gtgattgcag agggcgttga gactcaagaa
     3481 cagatgagtc tgcttgaaca aagcggttgt atgaatttcc agggttatct tttttcccgc
     3541 ccgctgcccg tcagtgaatt tgaaggcttg tttgcttagc tgtcttcttt agcgggagcg
     3601 tgttttctcc cgtgcgcggt gctgtcgttt cgcgtatcgg cggtcctgca atcagggcat
     3661 cttggtcacc tcgaagaatt caagctgcgg aggttcctga cgcgctccgg gagccgtggc
     3721 aggttgatag gccgagagct tgaacatcgt gccgctcggc aattcaaggc gcaccttgaa
     3781 gcgtttggtg atctgcaccg cgcccacgat cccggttctg acgaagcgat catccctgtg
     3841 cggcaactgc cacataaatt ccagcagcaa tgccggactg atgaatcccg cctgtagcgc
     3901 caaagtcatt tgggcaaagt cggcctccca ctgaatttcg caatcatgtg catcgggagt
     3961 gctgcttttt attacaacgc tgggcatgtg ttcctctgga aatatattcg agttgggttt
     4021 atctggactc gcgtgacagc gggctgcgcc agcgtttcag cagcagcgcg ttgctgacca
     4081 ccgacaccga gctcatcgcc atcgccgcgc ccgcgatcat cgggttgagc atgccgaacg
     4141 cggcgagcgg gatgccgagc acgttgtagc cgaaggcgaa gaacaggttc tggcgtatct
     4201 tggcgagcgt cgcgcgcgac agcgagatcg catccaccac gctggccagg tcgttgcgca
     4261 tcagcgtgat gtcggcggct tcgagcgcga tgtcgctgcc gctcttcatt gcgaagccga
     4321 catcggcggc ggccagagcg ggcgcgtcgt tgatgccgtc gcctgccatg ccgaccagtt
     4381 ttcctgcttg tttgtaagac tccaccagcg cggccttgtc ttgcggcagc acttcggcgc
     4441 ggaattctgt gatgcccgcc tgtgcggcga tcgcctcggc ggtggcgagg ttgtcgccgc
     4501 tgagcatcac cacgcgcacg cccatctcgt gcagtctggc gaccgcgttg cggctgctgg
     4561 ggcgcagccg gtcggcgagg gcgatcaggc cgagcagctg gccgtcgcgc gtcacgccga
     4621 tgacggtctt gccttgctgt tgcagcgctg tcagttgcga ctcgtcgagc gtgacgccct
     4681 gttgcgtgac ataggcgggc gagcccagca ggcagggctt gccgtcgagt tcggcgctca
     4741 gcccctgccc cgcatgttcg ttcaggttcg cgacttcggg gatgtcgatc tgtccggcat
     4801 ggtcgagcag cgcgcgcgac agcggatggc cggagtgttg cgcgagcgcg gcggcgacgc
     4861 gcagcagttg gtcttgtgcg acaccgtcga cgggcagcag gtcggtcacg ctgggcttgc
     4921 cttcggtcag cgtgccggtc ttgtccacca ccagcaccgc gagcttgtgc gcacgttcca
     4981 gtgcggcggc atccttgacc agtattccgg catgggcggc gcggcctgtt gccaccacca
     5041 cggcggtggg ggtggccaag cccagcgcgc agggacaggc gatgaccagc accgacaccg
     5101 cgttgatcag cgccggctcc cagtcgctgc ccgccagcag ccagccggcg aaggtgaggg
     5161 cggccaccaa gagcacgacc ggcacgaaga cggccgccac ctggtccacc agacgctgga
     5221 tgggcgcctt gccggcctgg gccgattcga ccagccggat gatgcgggcc agcgtggttt
     5281 cagccccgat ggcggtggtg cgcacggtca gcaccccctc gccattgatg gcgccaccgg
     5341 tcacccgact gtccaccgtc ttgagcaccg ggcggct
//
LOCUS       DASUIV010000297        16548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_77841, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000297 DASUIV010000000
VERSION     DASUIV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 16548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16548
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77841"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1094
                     /gene="lgt"
                     /locus_tag="VFW59_10640"
     CDS             312..1094
                     /gene="lgt"
                     /locus_tag="VFW59_10640"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX5364714.1"
                     /translation="MLTHPQFDPVALQLGPLAVHWYGLMYLAGFMTFLWLGRKRISAL
                     NHPKIDAKLLDDLLFYGVLGVILGGRLGYVLFYKAAYYADHPLEILSLWQGGMSFHGG
                     FLGVLIAMAWFAHKQNLRWLELTDFIAPLVPPGLAFGRLGNFINGELWGRPGDVPWAM
                     IFPQVDALPRHPSQLYEFALEGLLLFGLLWVYARKPRPIGAVSGLFLIGYGTLRFLVE
                     FTREPDNFLGLLSLGMSMGQWLSLPMVIAGVILMARSLRRAS"
     gene            1135..2406
                     /locus_tag="VFW59_10645"
     CDS             1135..2406
                     /locus_tag="VFW59_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX5364715.1"
                     /translation="MNDISSSSLLLILLLLVVFGAWFSAAETAMMAINRYRLNGLVRK
                     GSRSAKLTSRLLSKVDKLLGSILFGNTLLNVAAAAVTNVIIMRLFGAEELTLLMGTLG
                     ITFVLLVFSEIMPKIIAASHPERIALPSSYLLAPLITLFHPVVSIATALVRGMLGVLR
                     IKVQTDQSSQKMTLEELRGLVLDAEHFLPRKHQKMLLNLVDLERISVNDVMIPRNQIE
                     ALDINADERELRQQIITSHHTLLPVYADTPGNIIGILHLKRVPALLQETTLDVAQLRE
                     VLYEPYFIPSDTPLLKQLQHFQERQARLGLVVDEYGELLGLVTLENILEEIVGEFTTQ
                     SPSQTGKFLRQEDGSFLLEGSSSLRELNRKLGLHLPLKGAKTLNGLILEHLEDIPEAG
                     TSLKIANYPIEIIQTQDRSVKVARIFPAPRV"
     gene            2492..3709
                     /locus_tag="VFW59_10650"
     CDS             2492..3709
                     /locus_tag="VFW59_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5364716.1"
                     /translation="MAVAAKPNKSKETSFLWEGKDKTGRLVKGEMRAAGQAVVSAALR
                     RQGINIVKIKKLRSSGKPVTEKDISLFTRQLATMLKSGVPLLQAFDIVGKGHSNPSVA
                     RLLFDIKTDVETGSSLQQAFKKYPLYFDELYCNLIGAGEAAGILDTLLDRLATYKEKI
                     QAIKSKIKSALFYPVSIIAVAFIITAVIMIFVIPAFKELFSSFGADLPAPTLVVMAIS
                     DFFVSWWWAIFGIVGGGIYSFFYFWKRNKKMQGVMDRIMLKLPIFGELIRKASIARWS
                     RTLATMFAAGVPLVEAFDSVAGAAGNAVYFDATKYIQREVSSGNSLTVAMQNTEVFPS
                     MVLQMVAIGEEAGSLDAMLSKVADFYEAEVDDAVEALSSLMEPIIMVVLGTLIGGMVV
                     AMYLPIFKMGQVV"
     gene            3855..4724
                     /locus_tag="VFW59_10655"
     CDS             3855..4724
                     /locus_tag="VFW59_10655"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX5364717.1"
                     /translation="MNFLDLLQSSPLVFSGVCAVLGLMVGSFLNVVIHRLPKMMEHGW
                     QQQCAELRGEQPVETPQYNLVVPRSACPHCGHMIGAWENIPVFSYLLLRGKCKGCHAR
                     ISPRYPLIETVSGLLCGYAAWHFGFGWPAAGALLLIWALLALTAIDFDTQLLPDDITL
                     PLLWIGLLLNSFGTYTDLYSAVLGAVFGYLALWSVYWLFKLVTGKEGMGYGDFKLLAA
                     LGAWLGWQLLPLIIISSSLVGAAVGITLIIAARHGRNIPIPFGPYLAGGGLIALFWGQ
                     TLTQSYLQLLAVP"
     gene            4721..5410
                     /gene="coaE"
                     /locus_tag="VFW59_10660"
     CDS             4721..5410
                     /gene="coaE"
                     /locus_tag="VFW59_10660"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176263.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5364718.1"
                     /translation="MSPSEHADETPSQSTRLTENEPLIAGYQDGRLIVGLTGGIGSGK
                     STVAELFARHGAAIIDADEISHQLTQPAGAAIPAIRAAFGDLYIDPNGALNRARMRTL
                     VFADAEARQRLEQLLHPLILAQAKMRLSQAASAPYRILVVPLLAQAPEFRRLVQRVLV
                     VDCDERHQIERVVRHRGLTETEARAIIASQTPRTERQRLADDIITNEGDLSSLSAQVD
                     ALHQRYLENSD"
     gene            5480..6241
                     /gene="zapD"
                     /locus_tag="VFW59_10665"
     CDS             5480..6241
                     /gene="zapD"
                     /locus_tag="VFW59_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEX5364719.1"
                     /translation="MISYEYPLNEKVRTLLRLEDLFARIEHFTAQNSSMEHHAALTTL
                     FEILEVACRADLKSELLQELERQKRQLAALHNNPAISEDALDAILSEIEHTSTNLLAM
                     NGKVGQHLRENEWLMGIKQRASMPGGTCEFDLPSYHYWQEQSAELRQRDLRAWLAPLL
                     PIASGMHIVLRLLRESGKNLHFTAHNGTFQQMQGGRVAQMLRVSLSSHLDCIPEVGAN
                     KYAINIRFILANYAAKSSLFEQDVPFELTFCSLSS"
     gene            6241..6426
                     /locus_tag="VFW59_10670"
     CDS             6241..6426
                     /locus_tag="VFW59_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX5364720.1"
                     /translation="MQSKPIVTCPQCGKEHQWDTSNRFRPFCSERCKLIDLGKWANEE
                     YRVEQREQDLPEADHQA"
     gene            complement(6414..7400)
                     /locus_tag="VFW59_10675"
     CDS             complement(6414..7400)
                     /locus_tag="VFW59_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEX5364721.1"
                     /translation="MNSSVEQIKVVEVAAAVLQKPDGSFLLAQRPPGKIWAGYWEFPG
                     GKVEPGETTRHALVRELREELGIEVEAAYPWLTRVFTYPHAVVRLHFFRVTAWSGELH
                     PHEGQQFSWQLNPSRSPLGKGGFADSITVSPILPANAAVLRALELPSLYAISNVAELG
                     EAEFMRRLEAALQNGLQLVQLREKHLSRDELRGFALRAVSLAHEYGAKVLLNGDVALA
                     QEVGADGVQLTGGQLADLSERPPLGWCAASCHSAEQLRRAEELGCDFALLSPVLPTKS
                     HPGASHLGWERFATLAAGASIPVYALGGLMRADMETAWRQGAHGIALLRQAW"
     gene            complement(7467..8666)
                     /locus_tag="VFW59_10680"
     CDS             complement(7467..8666)
                     /locus_tag="VFW59_10680"
                     /inference="COORDINATES: protein motif:HMM:NF019379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 family protein"
                     /protein_id="HEX5364722.1"
                     /translation="MIIGLSKSFYAVKREPSVTTFHWLVPVIFAMVLVGCSGLPSKEP
                     EQAVAAPASSLPHKPAVKIAPRPKPLTGQALVSSLLPPALADRDGWAADIMAVFEVLK
                     IPPGKANICAVLAEIGQESSFQTDPVVFGLTRIVRDKLEASRTKYAIPQWVMKRSLAM
                     KSPDGHSYDERIDALKTENDLYLLYEDMVSEIPLGKKFLADFNPVQTVGPMQVSLSFA
                     NSYATKKHYPYAYEGPLRNALATRKGGLYFGVAYLLDYPASYDSIAFRFADFNAGQYS
                     SRNAAFQNALSSLTGKPLRRDGDLLRYKDGVALEDVSQTMQALLSISSRLGMGKAEIL
                     HNLLLEKSPAFEHSQLYTRVFALAPPMPRAYLPEIVVGGPKTSGMLTTAMYAKKVDGR
                     YRRCLSQ"
     gene            complement(8727..9527)
                     /locus_tag="VFW59_10685"
     CDS             complement(8727..9527)
                     /locus_tag="VFW59_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5364723.1"
                     /translation="METVELPLRIFIVDDEPPARSRLRDLLQDCSEQLALKVVGEAGN
                     GQEALDKLMVTPADVVLLDIRMPQMDGIELAQHLNRLPRPVLGEVEEHAPKWPPVIIF
                     TTAYDAYAIKAFELHAIDYLLKPIRLGRLFEALGRARAAVPLQLEVLRELLPEARKHI
                     SIHERGKIHLVPIDQVLFLRAELKYVTVRTAEQEYLLEESLSALEKEFSTRFVRIHRN
                     CLVAKEAIEGFEKGGEDGDGSGWLVKLKGLDERLAISRRQQYIVKEFG"
     gene            complement(9514..10566)
                     /locus_tag="VFW59_10690"
     CDS             complement(9514..10566)
                     /locus_tag="VFW59_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5364724.1"
                     /translation="MNKARSIKQNPLPDTLPNFRNLGVTLRILLAGNGLALMQALLQA
                     NAWADLPLLVMDISAMLTPVMLASLLVLWGVKPWLARLKYWQGVLAVNLLVVAVTLVI
                     YHLGGEFYHPSVHEDGWFGAVRYALLAAAVCVILLVYFRWRSQVLSFALHDARLQVLR
                     ARIRPHFLFNTLNAVLSIVRAQPRQAETALEDMADLFRMAMTEEQDLVPLRQEMQLSK
                     QYLALEQLRIGERLRVEWRMEALPDDVLIPPLLLQPLLENAVYHGIESLPQGGEVVVT
                     LQRRGDRLQISVENPCARREPAPHSGNHMALRNIRERLALLFDVEASYKVEKGDDFYR
                     VEIIMPYMKEKSRGNS"
     gene            10609..12000
                     /gene="argH"
                     /locus_tag="VFW59_10695"
     CDS             10609..12000
                     /gene="argH"
                     /locus_tag="VFW59_10695"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5364725.1"
                     /translation="MASIQDKDTWSGRFNEPVAELVKRYTASVGFDRKLALFDIRGSL
                     AHAQMLAACDIIAAQDLADIRRGLAQIENEVIADEFVWSLDLEDVHLNIEKRLTTLVG
                     DAGKRLHTGRSRNDQVATDVRLYLRDAIDDMLELIRHLQAALLDLATQHTRTIMPGFT
                     HLQVAQPISFAHHLMAYFEMLKRDAERLHDCRKRVNRLPLGAAALAGTSYPINREMVA
                     ELLGFEGVCENSLDAVSDRDFAIEFTAGAALIMTHLSRFSEELILWMSPRVGFIDIAD
                     RFCTGSSIMPQKKNPDVPELVRGKTGRVNGHLVALLTLMKGQPLAYNKDNQEDKEPLF
                     DTVETLTQTLRIYADMITGIRVKPEAMRNAALQGYATATDLADYLVKKGLPFRDAHEA
                     VALAVRYAEQRGCDLAEIELTELQRFSPDIADDVYQVLSLEGSLASRNHIGGTAPNQV
                     EAAIARAREHLAK"
     gene            complement(12057..12254)
                     /locus_tag="VFW59_10700"
     CDS             complement(12057..12254)
                     /locus_tag="VFW59_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364726.1"
                     /translation="MDNGNTGCKRFLLSGAGAVVLTILAFVSGANALGMVLVGVAIAS
                     FGYGAGTVMNSPEPMHKLNKD"
     gene            complement(12542..12856)
                     /gene="grxD"
                     /locus_tag="VFW59_10705"
     CDS             complement(12542..12856)
                     /gene="grxD"
                     /locus_tag="VFW59_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX5364727.1"
                     /translation="MTTDIQQRIREQVTTHPVVLYMKGNPQFPQCGFSANAVRILHAL
                     GVKEFFSVDVLQDAEIRQGIKDYANWPTIPQLYIKGEFVGGSDIMTEMYQSGELKQLL
                     AG"
     gene            complement(12924..13838)
                     /gene="ylqF"
                     /locus_tag="VFW59_10710"
     CDS             complement(12924..13838)
                     /gene="ylqF"
                     /locus_tag="VFW59_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEX5364728.1"
                     /translation="MAIQWFPGHMASARKKASETMERADVVIEVLDARVPEASSNPMI
                     KELRLHRQRPCLKLLNKADLADPVATKAWLEYFNSQKGVTAVALSCKKPSDVARVPNL
                     CLPLAPHRGTPLKALRMMIMGIPNVGKSTLMNALLKRRVANVGDEPAVTKSQQSLALN
                     DRMTLIDTPGMMWPKIEHDSDGLMLAASHAIGRNAVIDEEVATFLADLLLVRYPSLIA
                     ARYKIDVAGLDGASLIEAIAQRRGYRIRGGDYDLEKAGLMLLQDYRDGALGRISLETP
                     DSRREMLARHAERNAQEAAEDLSDEAGD"
     gene            14099..14992
                     /locus_tag="VFW59_10715"
     CDS             14099..14992
                     /locus_tag="VFW59_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5364729.1"
                     /translation="MSLPAAFVSVILIWSTTPLAIKWSALGIGSSFAVFSRMAIGTLL
                     CSALLLLLRVRFPLHRKAVLAYLVSGVSMFGAMALTYWSAQSVSSGMISVLFGLSPLL
                     TSLGAMWLLKEEALTPSKLAGILMGLLGLALVFRGALNLGEGAGLGLTALFLAVTLQS
                     SGLVWLKRIGDDSPPLATTLGSLLVALPLFFASWWLADGHWPTDLPVRAVAATVYLAA
                     VGSVLGFMLYYYMIKHMETGRIALITLITPVMALLLGQELNDEAVLPQVWLGTASILF
                     GLCLHRWGARWWDYATKMKGP"
     gene            complement(15003..15713)
                     /locus_tag="VFW59_10720"
     CDS             complement(15003..15713)
                     /locus_tag="VFW59_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5364730.1"
                     /translation="MSKMSLDRILQSQGFGTRKWCAELIADGDVIIGGAVVRDSRALI
                     ETDGLSFAVYGERWDYREHVYIALNKPANFECSRKPSHHPGVLTLLPEQFVRRDVQPV
                     GRLDHDTTGLLLLSDDGAFIHRQSSPKHHVPKLYVATTQDPVTPQLVAKLLAGVKLHD
                     EPLPLAAVSCRMLDTHRIEIVLEQGKYHQVKRMLAAAENHCVALCRTQIGELTLDGLG
                     EGEWRYLDAAQLDLLSPS"
     gene            15845..>16548
                     /locus_tag="VFW59_10725"
     CDS             15845..>16548
                     /locus_tag="VFW59_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364731.1"
                     /translation="MNLMFWKKKSPRDEEAGDTAPGLFARMKAHVAALTGRFRTPPPF
                     QAEVADDGNASRNDSADADTAMPETGKKSGSRTRLIVGGTILLLLLLATFGFATWKIF
                     LSSPDHDTDPLKSAAEQHPIASSRPIVIPASAPSSLPASAAAPETATLSAPAETQVSA
                     DAQLEALRQKKSELQGLLDDRKLEHPPAPAETEHAGKASGADDRIAASAEPKVTAHEK
                     SAASSVSQTRPLATAEP"
BASE COUNT         3525 a         5232 c         4507 g         3284 t
ORIGIN      
        1 gtggaggtgc gggaacggta tgggggcatg atgcggattc cgtaaataac aaagatggtg
       61 aattctaaac caggaggcca gcgctacgct gaagaatcaa actcaacggt tatgcacgtt
      121 gcagtatttc cccatcacgc cggaatgccg cacgaaatat cgcgccggga tttaatgccc
      181 cgcctcggcc cccatccatg acatgccaca gcctcgggcg aaccgcattc ttgcactcca
      241 cccctgcgaa gctccctggt atcgagccat ctccaagaat gagcgcacca atctgtgata
      301 aattgcgcgc catgctgacc cacccccaat tcgatcccgt cgccctccaa ctcggcccgc
      361 ttgcggtcca ctggtatggc ctgatgtatc tcgccggctt catgactttc ctgtggctgg
      421 gccgcaaacg catctccgcc ctgaatcatc ccaagatcga cgccaaactg ctggatgatt
      481 tgctgttcta tggagtgctc ggcgtgatac tcggcggccg tctgggttat gtgctgttct
      541 acaaggccgc gtattatgcc gatcatccgc tggagattct gtcgctgtgg cagggaggca
      601 tgtcgttcca cggcgggttc ctcggcgtat tgatcgcaat ggcctggttc gcgcacaaac
      661 agaatctgcg ctggctggaa ctgaccgact tcatcgcccc gctggtgccg cccggtcttg
      721 cattcggccg gctgggcaac ttcatcaatg gtgagttatg gggacgtccc ggcgacgtgc
      781 catgggcaat gatcttcccg caagtggatg cactgccgcg ccatccttcg caactgtacg
      841 aattcgcgct ggaaggcttg ctgctgttcg gcctgctttg ggtctatgcg cgcaagccgc
      901 gccctatcgg cgcggtatcc ggcctgttcc tgatcggtta tggcacgctg cgcttcctgg
      961 tcgagttcac ccgcgaaccg gacaatttcc tcggcctgct gtcgctgggc atgagcatgg
     1021 ggcaatggct gagcctgccc atggtgattg ccggcgtgat tctgatggcg cgcagcctgc
     1081 gtcgcgccag ttgacccaga cgcatcttct ccaatccatt gcatccccgt tgacttgaac
     1141 gacatctctt cctcctccct gctgttaatc ctgctcctgc tggtggtttt cggcgcatgg
     1201 ttctcggccg ccgagacggc catgatggca atcaatcgct atcgactgaa cggcctggtt
     1261 cgcaaaggca gccggagcgc aaagctgacg tcccgactgc tctccaaggt agacaagctg
     1321 ctcggctcga tcctgttcgg caataccctg ctgaatgtgg cggcggcagc cgtcaccaac
     1381 gtgatcatca tgcggctgtt cggcgccgag gaactgaccc tgctgatggg gacgctcggc
     1441 atcaccttcg tgctgctggt gttcagcgag atcatgccca agatcatcgc cgccagccat
     1501 cccgagcgca tcgcgctgcc ttccagctac ctgcttgcgc cactgatcac gctgtttcat
     1561 cccgtcgtat ccattgcgac cgccctcgtc cggggaatgc tgggcgtgct ccgcatcaag
     1621 gttcaaaccg accaaagcag ccagaaaatg acgctggagg aactgcgcgg actggtgctg
     1681 gatgccgaac acttcctgcc gcgcaaacac cagaagatgc tgttgaatct ggtcgacctc
     1741 gaacgcatca gcgtgaacga cgtgatgatc ccgcgcaacc agatcgaggc gctggacatc
     1801 aacgccgatg agcgcgagtt gcgccagcag atcatcacca gtcaccacac cctgctgccg
     1861 gtatatgccg acactccggg caacatcatc ggcatccttc acctcaagcg cgttccggcc
     1921 ctgctgcagg aaaccacgct ggacgtggcg cagctgcgcg aggtgctgta cgagccctat
     1981 ttcatcccct cggacacgcc actgctcaag caactgcagc atttccagga gcggcaggcc
     2041 cgcctcggcc tggtggtgga tgaatacggc gaattgctgg gactggtcac gctggaaaac
     2101 atccttgaag agatcgtcgg ggaattcacg acccagtcgc catcacagac cggaaaattc
     2161 ctgcgtcagg aagacgggag ctttttgctg gagggcagca gcagcctgcg cgaactgaat
     2221 cgcaagctcg gattgcatct gccgctgaaa ggagcgaaga cgctcaatgg actgatactc
     2281 gagcatctgg aagacatccc ggaagccggc accagtctga aaatcgccaa ctatccgatc
     2341 gagatcatcc agacccagga tcgcagcgtg aaggtcgcgc gcatcttccc tgcgccccga
     2401 gtataagcac atgaggaatc gtacctttac aagcaacaca aagctcggca tcatgcccgt
     2461 caatattggt tagtgtcccg agggaaatac catggcagtc gctgcgaaac caaacaaatc
     2521 aaaagagacg tccttcctct gggaaggcaa ggacaagacc ggcagactgg tcaagggtga
     2581 aatgcgtgcc gccggccagg cagtcgtctc ggccgcgctg cgccgccaag gcatcaacat
     2641 cgtcaaaatc aagaagctgc gcagcagcgg gaagcccgtc accgaaaaag acatttcgct
     2701 gttcacccgc cagctcgcca ccatgctgaa gtccggcgta ccgttgctgc aagccttcga
     2761 catcgtgggc aaaggtcaca gcaacccctc ggtcgctcga ctgctgttcg acatcaagac
     2821 cgacgtggaa accggcagca gcctgcaaca agccttcaag aaatatccct tatattttga
     2881 cgaactgtac tgcaacctga tcggcgctgg cgaagcggcg ggtattctgg atacactgct
     2941 ggaccggctg gcgacctaca aggaaaagat tcaggctatc aagagcaaga tcaagtcggc
     3001 gctgttctac cccgtttcta tcattgcggt cgccttcatc atcactgcag tgatcatgat
     3061 cttcgtcata ccggcattca aggagctatt ctccagcttt ggcgctgacc tgcctgctcc
     3121 aacgctagtc gtcatggcga tttccgactt cttcgtctcc tggtggtggg ccatattcgg
     3181 catcgtcggc ggcggcatat acagcttctt ctacttctgg aagcgcaaca aaaagatgca
     3241 gggcgtgatg gatcgcatca tgcttaagtt gcccattttc ggcgaactga tccgcaaggc
     3301 cagcatcgcg cgctggagcc gtactctggc caccatgttt gccgctggtg tccccctggt
     3361 cgaggccttt gactccgtcg ccggcgcggc aggcaatgcg gtgtacttcg atgcgaccaa
     3421 atacattcag cgcgaagtca gttccggcaa cagcctgacc gttgccatgc agaacaccga
     3481 ggtattcccc agcatggtgc tgcaaatggt cgccatcggc gaggaagccg gttcgctgga
     3541 tgcgatgctg agcaaggtgg ccgacttcta tgaagccgaa gtggatgatg ccgtcgaagc
     3601 gctatccagc ctgatggaac cgatcatcat ggtggttctc ggcacgctga tcggcggcat
     3661 ggtggtcgcc atgtacctgc cgatcttcaa gatgggccaa gtggtttaac aggatggggg
     3721 gtggcgagta ggccgacgct gcacgtctca gcaggaagtg gaaaaagccc gtcccccact
     3781 tcctgcctat ctcttccact ctccttgctg cgcgctgccc aacacccact ttccactccc
     3841 tacttccccc caagatgaat ttcctagatc tgctccaatc ctcccctctg gtgttttccg
     3901 gcgtctgcgc tgtgctgggc ttgatggtcg gcagtttttt aaacgtcgtg atccaccggc
     3961 tgcccaagat gatggagcat ggctggcaac aacagtgcgc cgaactgcgt ggcgaacagc
     4021 ccgtcgagac gccgcagtac aatctggtcg tgccgcgctc tgcgtgcccg cattgcggcc
     4081 acatgatagg ggcatgggaa aacattccgg tcttcagcta tctgctgctg cgcggcaagt
     4141 gcaaaggctg ccacgcgcgt atttctccac gctaccccct gatcgagaca gtcagcggcc
     4201 tgctatgcgg ttacgcggca tggcacttcg gtttcggctg gccggcagca ggcgctctgc
     4261 tgctgatatg ggcattgctt gccctgaccg ccatcgattt cgatacccag ttgctgcccg
     4321 acgacatcac cctgccgctg ctctggatcg gcctgctgct caacagcttt ggcacctaca
     4381 ccgacctgta cagcgcggtg ctgggcgcgg tcttcggcta tctcgcgctg tggagcgttt
     4441 actggctatt caaactcgtc acgggcaaag aaggcatggg ctatggcgac ttcaagctgc
     4501 tagccgcact gggcgcctgg ctggggtggc agttgctgcc actgatcatc atctcttcct
     4561 cgctggttgg cgcggcagtc ggcatcaccc tgatcattgc cgcccgtcat ggccgcaaca
     4621 tccccatccc gttcgggccg tatctggccg gcggcgggct gatcgcgctg ttctgggggc
     4681 aaaccctgac acaaagctat ttgcaactgc tcgccgtacc atgagcccct ccgagcatgc
     4741 cgatgaaacg cctagccaat cgaccagact gacagaaaac gaacctctga tcgctggtta
     4801 tcaggacggg cggctgattg tgggactcac cggtggcatc ggcagcggaa aaagcacggt
     4861 agccgagctg tttgcccgac atggcgccgc catcatcgac gcagatgaaa tctctcacca
     4921 attgacccaa cccgccggcg cggccattcc cgcgattcgc gccgccttcg gcgatctcta
     4981 tatcgacccg aatggcgccc tgaatcgcgc aaggatgcgc actctggtat ttgcggatgc
     5041 cgaagccaga cagcgcctgg aacaactgct gcatccgctc atcctcgcac aagccaagat
     5101 gcgactgtca caggcagcct cggctcccta ccgcatcctc gtcgttccgt tgttagccca
     5161 agctccggaa tttcgccgat tagtgcaacg tgtactggtc gtggactgcg acgaacggca
     5221 ccagatcgaa cgcgtcgtcc ggcaccgagg actgaccgag acagaggctc gcgcgatcat
     5281 cgccagccag acgccgcgaa ccgaacgtca aagattagct gacgatatca tcaccaacga
     5341 aggcgatctg agcagcctgt ccgcacaggt tgatgccctc caccagcgtt atcttgaaaa
     5401 cagcgattga cgacatgacg gcatccaata tatcgttcgc aacatatatc ccaaccacga
     5461 cccaaacagc gataacggcg tgatcagcta cgagtatcct ctaaacgaaa aggtacgcac
     5521 tttgttgcgt cttgaggact tgttcgctcg cattgagcac ttcactgcac aaaatagcag
     5581 catggagcat cacgccgcac tcacgacgct gttcgaaatc ctcgaagttg cctgtcgcgc
     5641 cgacctgaag tcggaactgt tacaggaact ggaacgccag aaacgccaac tcgccgctct
     5701 gcacaacaac ccggccattt ccgaagacgc actggatgcc atcctgtcgg agatcgagca
     5761 cacctcgacg aacctgcttg cgatgaacgg gaaggtcggg cagcacctgc gcgagaatga
     5821 atggctgatg ggcatcaaac aacgtgccag catgccaggc ggcacttgcg aattcgattt
     5881 gccttcgtat cactactggc aagaacaaag cgccgaattg cgccaacgcg acttgcgcgc
     5941 ttggctggcc ccgctactgc ccattgcctc cgggatgcac atcgtgctgc gcctgctgcg
     6001 cgaaagcggc aagaacctgc acttcaccgc tcacaatggt acattccagc aaatgcaggg
     6061 agggcgcgtt gcacagatgc tgcgcgtcag cctgagcagc cacctcgact gcattccgga
     6121 agtgggcgcg aacaaatacg ccatcaacat ccgtttcatc ctcgccaact atgcggcgaa
     6181 atcgagcctg ttcgaacagg atgtcccctt cgaattaacc ttctgcagcc tgtcgtcgta
     6241 atgcaatcga aaccgatcgt cacctgcccg caatgcggca aagagcacca atgggatacc
     6301 agcaaccgct ttcgcccatt ctgcagcgag cgctgcaaac tcatcgatct ggggaaatgg
     6361 gcgaacgaag aatatcgcgt cgaacaacgc gagcaagatt tgccggaagc ggatcaccag
     6421 gcctgacgca acaaagcgat gccgtgcgcg ccctgccgcc aggccgtttc catatcggcg
     6481 cgcatcaagc cacccaatgc atacaccggg atcgatgctc ctgcggcgag cgtggcaaaa
     6541 cgctcccacc ccagatgaga cgccccagga tgcgacttgg tcggcagcac cggactcaat
     6601 agcgcgaagt cgcaccccag ctcttccgcg cggcgcaact gctcggcaga atgacaggag
     6661 gctgcacacc agcccaacgg cgggcgctca ctcaagtcgg ccagctgccc ccccgtcagc
     6721 tgcaccccgt ctgcaccgac ctcctgtgcc aaagccacat ccccattaag caacaccttc
     6781 gccccatact catgcgccag cgacaccgcc cgtagcgcga acccgcgcag ctcgtcgcgg
     6841 gaaagatgct tctcgcgcag ctgcaccagc tgcaagccat tctgcaatgc ggcttccagt
     6901 cgcctcatga attcggcctc ccccagctcg gcgacattgc tgatcgcata caacgaaggc
     6961 aactccagcg cgcgcagcac tgccgcattg gccgggagga tgggcgagac cgtaatcgaa
     7021 tccgcgaaac ctcctttgcc caggggggaa cgggaaggat ttaactgcca agaaaactgc
     7081 tgcccctcgt gcggatgcag ctcgccgctc catgccgtca cgcgaaagaa atgcagccgg
     7141 acaaccgcat gcggataggt gaacacgcgg gtgagccacg ggtaggcggc ctcgacctcg
     7201 atgccgagct cttcgcgcaa ctcgcgcacc agcgcgtggc gcgtcgtttc gccgggctcg
     7261 accttcccgc ccggaaactc ccaatatcct gcccagattt tgccgggggg gcgctgggcg
     7321 agcaggaagc tgccgtctgg tttttgcaaa acggcggcgg cgacttcgac gactttgatt
     7381 tgttcaacac tggaattcaa tgaaacctca tttctgacag accgttcgta tcgacaacca
     7441 gcggcaggat cgaatgcacc gcccgttcat tggctcaaac acctgcgata gcgcccatct
     7501 accttcttgg catacatagc cgtcgtcagc atcccggagg tcttgggtcc gcctaccacg
     7561 atttccggaa ggtatgcgcg cggcatcggt ggcgccaagg caaacaccct tgtgtataac
     7621 tggctgtgct caaacgcagg ggacttctcc agcaacaggt tatgcaaaat ctcagcctta
     7681 cccatcccca gacgcgatga gatcgagagc agggcttgca tggtctgact gacgtcctca
     7741 agcgcaacgc cgtctttata gcgcagcaag tcgccatcgc gtctcagcgg cttgccggtc
     7801 aaggaactca gtgcattctg gaacgctgcg ttcctgctgg aatattgtcc cgcattaaaa
     7861 tcggcaaagc gaaaagcaat gctgtcataa ctcgccggat aatccagcag ataggcgact
     7921 ccaaaataca gcccgccctt gcgggtcgcc agtgcattgc gcaagggccc ttcgtaggca
     7981 tagggatagt gtttttttgt cgcatacgaa ttggcaaagc tcaaactgac ctgcatcggg
     8041 ccaacggttt gcacgggatt gaaatcagcc agaaacttct tccccaacgg aatctccgag
     8101 accatgtctt cgtagagcag atacaaatca ttttccgttt tgagcgcatc gatgcgttcg
     8161 tcatagctat gcccatcggg cgatttcatc gccagactgc gcttcattac ccactgggga
     8221 atggcgtact tcgtccgact cgcttccagc ttgtctctaa cgatcctcgt cagcccgaaa
     8281 acaacggggt cggtctgaaa cgacgactct tgtccgatct ccgccaatac cgcacaaata
     8341 ttcgcttttc ccggaggaat tttcagcacc tcgaacaccg ccataatatc tgcggcccaa
     8401 ccgtcgcgat ctgccagtgc ggggggcagc aaactactca ccagcgcctg cccggtcaat
     8461 ggctttggcc ggggcgcgat cttgaccgcc ggcttatgcg gcagcgacga tgctggcgct
     8521 gcaacggcct gttcgggctc ctttgagggg agcccggaac acccgaccag caccatcgca
     8581 aaaatcaccg ggaccaacca atggaacgtt gtgactgaag gctcgcgctt tactgcgtaa
     8641 aaagacttgc tcagaccaat tatcattact tttacttgag acttactcta gccaggcggg
     8701 gttaaaaccg gaagccccct cgccactcag ccaaattcct tgacgatata ttgctgccgc
     8761 cgactgattg cgagcctctc gtccaaaccc ttcagcttga ccagccagcc gctgccatca
     8821 ccatcctccc cgcctttctc gaaaccttcg atagcctctt tcgctaccag acagttgcga
     8881 tggatgcgca caaagcgggt tgaaaactcc ttctccagcg cgctgagcga ctcctcgagc
     8941 agatactcct gttctgcggt gcgcaccgtg acatatttca gctcggcgcg caggaacagc
     9001 acctgatcga tgggaaccag atgtatcttg ccgcgctcat ggatagatat atgtttgcgg
     9061 gcctcgggca ggagttcgcg caatacttca agttgcaacg gcaccgccgc gcgtgcgcgc
     9121 ccgagcgctt caaacagccg ccccagccgg ataggcttga gcaggtaatc gatcgcatgc
     9181 aattcgaatg ccttgatcgc ataagcgtcg tacgcagtgg tgaaaataat caccggcggc
     9241 cacttcggcg catgctcttc gacctcgccc agaacgggcc gaggtagcct gttgagatgt
     9301 tgcgccagct cgatgccatc catctgcggc atgcgaatat ccagcagcac cacgtcggcg
     9361 ggcgtcacca tcagcttgtc cagcgcttcc tgtccgttgc ctgcctcacc caccaccttc
     9421 aaggccagct gttcactgca gtcttgcagc aagtcgcgca agcggctacg cgcgggcggc
     9481 tcatcgtcca cgatgaagat acgcaggggc agctcaactg tttccacggc tcttctcctt
     9541 catgtagggc atgatgattt ccactcgata gaagtcatcc cctttttcaa ccttgtaact
     9601 tgcctcgaca tcaaacagca acgctaaccg ctcacggata ttgcgcaatg ccatgtgatt
     9661 gccggaatgt ggcgcgggtt cgcgtctcgc acagggattc tccacgctga tctgcaatct
     9721 gtcccccctg cgctgcagcg tcacgacgac ctcgcctcct tgcggcaacg attcgatgcc
     9781 gtgatacacg gcattttcca gcaacggctg caacaatagc ggcgggatca gcacatcgtc
     9841 cggcaatgcc tccatacgcc actccacccg caagcgttcg ccgatacgca actgctcaag
     9901 agcgagatac tgcttgctca actgcatctc ctgacgcagc ggcaccagat cctgctcctc
     9961 ggtcatggcc atacggaaca aatcggccat gtcctccagc gccgtctcgg cctgccgtgg
    10021 ctgggcccgc acgatactga gaaccgcatt aagcgtattg aacaaaaaat gggggcgaat
    10081 gcgtgcgcgc aacacctgca aacgggcatc gtgcaaagca aacgacaata cctgactgcg
    10141 ccagcgaaaa tacaccagca gaataacgca aacagccgca gcaagcagcg catagcgaac
    10201 cgcgccgaac catccatctt catgcaccga tggatggtaa aactccccgc ccagatgata
    10261 gatcaccaac gtcacggcaa ccaccagcag gttcaccgcc aacacgcctt gccaatattt
    10321 caggcgtgcc agccatggct tcacccccca tagcaccagc aggctggcca acataacggg
    10381 cgtgagcatc gctgagatgt ccatcaccag cagcggcaaa tcagcccacg cattcgcctg
    10441 cagcagcgcc tgcatcaatg ccagaccatt gccggccaac agaatgcgca aggtgacgcc
    10501 gagattgcga aagttcggca acgtatcggg cagggggttt tgttttatac tgcgcgcctt
    10561 attcatggcg agaatcctca ggttttgagc cacaattctg gacaagacat ggctagcata
    10621 caagataaag atacatggtc gggacgtttc aacgaacccg tggctgaact ggtgaagcgt
    10681 tataccgcgt cagtcggctt cgaccgcaaa ctggcgctgt tcgatatccg tggctcgctg
    10741 gcgcacgccc agatgctggc ggcttgcgac atcatcgccg cacaggatct cgccgacata
    10801 cggcgcggac tcgcacagat cgagaacgaa gtgatcgccg acgaattcgt ctggtcactg
    10861 gacctcgagg acgtgcatct caatatcgaa aaacgtctca ccacgctggt cggcgatgcg
    10921 ggcaaacgcc tgcacactgg ccgctcgcgc aacgatcagg tcgccaccga tgtgcgcctg
    10981 tatctgcgcg acgccataga cgacatgctc gaactcatca ggcatctgca agccgctttg
    11041 ctcgaccttg ccacacaaca cacccgcacc atcatgccgg gcttcaccca tttgcaggtg
    11101 gcgcagccga ttagcttcgc acaccacctg atggcgtatt tcgagatgct caaacgcgac
    11161 gcggagcgcc tgcacgattg ccgcaaacgc gtgaaccgtt taccgctggg cgcagccgca
    11221 ttggccggca ccagctatcc gatcaatcgc gagatggtcg ccgaactgct gggcttcgag
    11281 ggcgtatgcg agaactcgct ggatgccgtg tcggatcgcg atttcgcgat cgaattcact
    11341 gccggcgcag cactgatcat gacccacctg tcgcgtttct ccgaggaact gattttgtgg
    11401 atgagcccgc gagtcggctt catcgatatc gccgaccgct tctgcaccgg gtcctccatc
    11461 atgccgcaga agaagaatcc ggatgtgccg gaactggtgc gcggcaagac gggacgcgtt
    11521 aacggccatc tggtcgcact gctgacgctg atgaagggcc agccgctggc ctacaacaag
    11581 gacaatcagg aagacaagga gccgctgttc gacacggtgg agaccttgac ccagaccctg
    11641 cgcatctatg ccgacatgat caccgggatc agggtcaagc cggaggccat gcgcaatgcg
    11701 gccttgcagg gctatgccac ggccaccgac ctggccgatt atctggtaaa gaaaggcttg
    11761 ccgttccgcg acgcgcatga agccgtggca ctggcggtac gctatgccga acaacgcggc
    11821 tgcgatctgg cagagatcga attgacggag ttgcaacgtt tctcaccgga tatcgccgat
    11881 gatgtctatc aggtgttgtc gctggaaggc tcgctcgcca gccgcaacca catcggcggc
    11941 accgccccca accaggtgga agcagccatc gcacgggcgc gcgagcacct ggccaaataa
    12001 aaacgccggg ggatgcactt agcatccacc ggcgccggtg aacaagactc gaacgatcag
    12061 tctttattca atttatgcat cggttcgggt gaattcatca ccgtgcctgc gccataacca
    12121 aaactggcaa tcgccacgcc caccagcacc atccccagcg cattcgcgcc ggaaacaaat
    12181 gccagaatcg tcaataccac cgcgccagcg ccggacaaca aaaagcgctt gcaaccggtg
    12241 ttcccattgt ccatattgtt ttccataccc atgatattcc ccctcggatt attgtattag
    12301 gtcgataatg actcgcacct cggcgaggat tcttgcccag ttcgtgtttt tgcgcaaccg
    12361 tcaaaaaatc cgggcaaaaa aaacgggcga tcatcagatc gcccgaacag gaggagagta
    12421 tgaaaaatcg tgtcgcgatc aaccaccatt tccaacactc ggtgcgcact atatcgcccc
    12481 agccggccaa agcccatata taggaatacc tatatatttc ggcctagcaa ctcctgcgga
    12541 cctagcctgc cagcagttgc ttcagctctc cgctctgata catctcggtc atgatgtccg
    12601 aaccgccgac aaactctccc ttgatataga gctgcgggat ggtcggccag ttggcataat
    12661 ccttgatgcc ctgacgaatc tcggcatcct gcagcacatc cacgctgaaa aactctttca
    12721 cccccagcgc atggaggatg cgtaccgcat tggcggaaaa gccgcattgc ggaaactgcg
    12781 gattgccctt catgtacaac accaccggat gagtcgtcac ctgctcgcgg atgcgctgct
    12841 gaatatctgt agtcataaga tcattacctg aattaaacag tcgggaataa tatcggtcgc
    12901 ggcgaggacg agtcaagcaa caatcaatcg ccggcctcat cggacaggtc ttcagccgcc
    12961 tcttgcgcgt tgcgttcggc gtgccgcgcc aacatctcac ggcggctgtc cggcgtctcc
    13021 agactgatgc gccccaaggc gccgtcacga tagtcctgca acaacatcag cccggccttc
    13081 tccagatcgt aatccccccc cctgatgcga tagccgcgcc gctgagcgat ggcttcgatc
    13141 agcgatgcgc catccaggcc cgccacatcg attttgtagc gcgcggcgat cagggacgga
    13201 tagcgcacca gcaacagatc ggcgaggaaa gtcgcaacct cttcgtcaat caccgcattg
    13261 cggccgatgg catgactggc ggccagcatc aggccgtcgc tatcgtgctc gatcttcggc
    13321 cacatcatcc cgggcgtgtc gatcaaggtc atacgatcgt tgagcgcaag gctctgctgg
    13381 ctcttggtca cggcgggttc gtcacccaca ttggccacgc gacgcttcag cagcgcattc
    13441 atcagcgtgg atttgccgac gttggggatg cccatgatca tcatgcgcag cgccttgagt
    13501 ggcgtgccgc gatgcggcgc cagcggcaaa cagaggttgg gcactctggc gacatcgctc
    13561 ggtttcttgc acgacagcgc gacagccgtc acacctttct ggctgttgaa gtactcaagc
    13621 cacgccttgg ttgcgacggg atcggccaga tcggccttgt tcaacaactt cagacagggg
    13681 cgctggcgat gtaggcgcaa ctccttgatc atcgggttgc tgctcgcttc cggtacgcgc
    13741 gcatccagca cttcgatgac gacatcggcg cgctccatgg tctcgctggc cttcttgcgc
    13801 gcggaggcca tgtgaccggg aaaccattga atagccataa tgtgagtctt gattgaataa
    13861 ggctcgcatt ctacccgtat ccgcagaacg cattgacttc ccccgtggcg ctcccgataa
    13921 tcccgcacca tgagatcgtc attttccatt cgtccggttc gagtccgccc atgcccactg
    13981 cgcatggcgt ggcattgcgc gcccggtggc caggaaagtt agcgttcaag cattgattgc
    14041 agccgcttcc cgcaaaggcc acagacactg tggccttttt tgtttttgga gaaaattcat
    14101 gtcgcttcct gccgccttcg tctcggtgat cctgatctgg tccaccaccc cgctcgccat
    14161 caagtggagc gcgctcggca tcggctccag ttttgcggtg ttctcgcgca tggcgatcgg
    14221 cacactgcta tgcagcgcgc tgctcctgtt gctgcgcgtg cgctttccgc tgcaccgcaa
    14281 ggccgtgctg gcctatctgg tcagcggcgt cagcatgttc ggcgccatgg cgttgaccta
    14341 ttggtcggca caatccgtca gctcgggcat gatctctgtg ctgttcggcc tgtcgccgct
    14401 gctcaccagc ctcggtgcga tgtggctgct caaggaagag gcgctcacgc ccagcaaact
    14461 ggcggggata ctgatgggtc tactgggtct ggcgctggtg tttcgcggcg cactgaacct
    14521 gggggaggga gcggggctcg gactgaccgc cttatttctc gccgtgacct tacaatcgtc
    14581 gggactggta tggctcaaac gcatcggcga cgacagccca ccgctggcaa ccacgctggg
    14641 cagtctgctg gtggccctgc ccttgttctt cgcctcatgg tggctggcag acgggcactg
    14701 gccaaccgat ctccccgtgc gtgcagtggc ggctaccgtc tatctggcgg cagtcggttc
    14761 ggtactcggc ttcatgttgt attactacat gatcaaacac atggagaccg gacgcatcgc
    14821 gctcatcacg ctgatcaccc cggtgatggc cttgctgctg ggacaggagc tgaacgacga
    14881 ggccgtgtta ccccaggtgt ggctgggcac ggccagcatc ctgttcggcc tgtgcctgca
    14941 tcgctggggg gcaaggtggt gggactatgc gacgaagatg aaaggaccgt agcgttgcct
    15001 gactagctcg gtgacaacaa atcgagctgt gccgcatcca gatagcgcca ctcgccctcg
    15061 cccagcccat ccagcgtcaa ctcgccgatc tgcgtacggc acaacgcaac acaatgattc
    15121 tctgcggcgg ccagcatgcg cttgacctga tggtatttcc cctgctccag cacgatctcg
    15181 atgcggtgcg tatccagcat gcggcagctg accgccgcca gcggcaaggg ctcgtcgtgc
    15241 agcttcacgc ccgcgagcag cttggccacc agttgcggcg tcaccggatc ctgcgtggtg
    15301 gccacataaa gtttgggcac gtgatgcttg ggcgaggact ggcgatgaat gaacgcgccg
    15361 tcatccgaca gcagcaacag accggtcgtg tcatgatcga gacgtcccac cggctgcaca
    15421 tcacggcgca caaattgctc cggcaacagc gtcagcacgc cgggatgatg actgggcttt
    15481 ctggagcact cgaaattggc aggcttgttc agcgcgatgt acacatgctc gcgatagtcc
    15541 caacgctcac catatacggc gaaactcaat ccgtccgttt cgatcagggc acggctgtcg
    15601 cggacgaccg cgccgccgat aatcacatca ccatcggcga tcaattcggc gcaccacttg
    15661 cgggtgccaa aaccctgcga ctgcaggatg cgatccagac tcattttgct catggcggca
    15721 ctttagcaga agccgcacct cgttgcccgt atcgacgcat cggcttgcgt gcgtttgacg
    15781 ctggagggat ggcgcaatat gctgcgcgct ggcggctgag catgccgcat gcaatggagt
    15841 cacgatgaat ctgatgttct ggaagaagaa atccccacga gatgaagagg cgggcgacac
    15901 cgcacccggc ttattcgcac ggatgaaagc gcacgtcgcc gcactgaccg gacgcttcag
    15961 aacaccgcca ccatttcagg ctgaagtggc cgatgacggg aacgcatctc gcaacgattc
    16021 cgccgatgct gacacggcga tgccggagac cggcaaaaaa tccggctcac gcaccaggct
    16081 gatcgttggc ggaaccattc tgctgttact cctgctggcc accttcggtt tcgccacctg
    16141 gaaaattttc ctgtcctcac ccgaccacga caccgacccg ctcaagagcg ccgcagagca
    16201 gcatcccatc gcatcatccc ggccgattgt gatacctgcc agcgcaccat cgtcactccc
    16261 cgcgtcggcg gcggcaccgg aaacggcaac cctgtcggcc cctgctgaaa cccaggtatc
    16321 ggctgatgcc cagctcgaag cgctcagaca gaaaaaatcc gagttgcagg gcttgctcga
    16381 cgaccgaaaa ctggaacatc cccctgcgcc agccgaaacg gagcatgccg gcaaagcaag
    16441 cggtgcagac gatagaatcg cagcatctgc cgagcccaag gtaaccgcgc acgaaaaatc
    16501 tgcagcctcg tccgtctcac agacccgccc tctggcaacc gccgagcc
//
LOCUS       DASUIV010000298         4334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1195494, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000298 DASUIV010000000
VERSION     DASUIV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195494"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFW59_10730"
     CDS             complement(<1..675)
                     /locus_tag="VFW59_10730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5364732.1"
                     /translation="MAAPSAPSHIGNYSIVEQIGTGASSNVYLAIEEGNFVSVAIKQL
                     RKTCQTEPYRRLMSNEVALVGKLHHKNIVRLLSANFTEKSGPYLVMEYIRGVSLQQHQ
                     HSDTLLPINTVISIVEQIAQALRYIAGQGVVHRDVKPENIILMPNGQAKLTDFGCAIP
                     TGSSGDMVAGSLAYMSPEQLEGQPLDERADIYALGAVLYRLLTGKNTFEAENEFDARI
                     AILNFPI"
     gene            complement(752..1051)
                     /locus_tag="VFW59_10735"
     CDS             complement(752..1051)
                     /locus_tag="VFW59_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364733.1"
                     /translation="MKSTDRALIEQNALFNGMDFESVEYMLEHSLVRNLEAGERLLQP
                     NIQSHQQHRILEGKLIAQVIEEHLSLLPDGCAGADQALYHAKQRGRNRVEVFALQ"
     gene            complement(1100..1963)
                     /gene="purU"
                     /locus_tag="VFW59_10740"
     CDS             complement(1100..1963)
                     /gene="purU"
                     /locus_tag="VFW59_10740"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX5364734.1"
                     /translation="MPHHYTLTISCPDRAGIIAAVSGFIAQHGGFIVEASYHTDQETQ
                     RFFMRQEVRADSLPFDAHEFKNRFGTLAKEFAMDWQLSDSAIKKRLVILVSKQDHCLN
                     DLLHRWRSGELLVDIPCVISNHGDLRSFVEWHGIPFIHVDMPANDPAAKATAFEHIAA
                     LFEQHRGDCLVLARFMQILPPFLCQRFPGRIINIHHSFLPSFVGAKPYHQAFHRGVKL
                     IGATCHFVTDELDAGPIIEQDTVRVDHGDTVDDLVRYGRDIEKTVLSRGLRYHVEDRV
                     LVCGNKTIVFR"
     gene            complement(2053..2811)
                     /locus_tag="VFW59_10745"
     CDS             complement(2053..2811)
                     /locus_tag="VFW59_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5364735.1"
                     /translation="MDARNNEIDFLASVTHDLKSPLNAILCTIDMLKMHIASENLDRD
                     AMLRNLSIAEMAGSDMLALVNNMLTTARMHAGKETADPALLGRAELIERTRNMERTFH
                     NEALGKNIDFSVTIGTLPEYVYWDIQKIRFFAVNNLISNALKFLGHGGTVKVLIDSDA
                     DNNVLISVMDDGPGIPVSERAGVFGKFVQASNNARSFQGGGFGLFNAHQTIAMHHGSI
                     EILDGLNGKGVTFQIKLPAIPFEFDEITAIQMAA"
     gene            complement(3019..3822)
                     /gene="murI"
                     /locus_tag="VFW59_10750"
     CDS             complement(3019..3822)
                     /gene="murI"
                     /locus_tag="VFW59_10750"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX5364736.1"
                     /translation="MSLHSGAIGVFDSGVGGLSVLHHIRRTLPHERLIYVADSGHVPY
                     GDKPASYIEQRSLLLTRFLIEQGADAIVIACNTATAAAAATLRSQFDLPILAMEPAVK
                     PAVAATRSGVVGVLATIGTLESARFAALLEKYAGEVEIVTQGCPGLVEQIERGDLDGP
                     ETRALLERYTAPLLACGADTLILGCTHYPFLAPLIREIAGDSIALIDTGEAVARHLQR
                     RIQTELTGRTQADASVQFFTSGDAAQASDIMSLLWGETVTAQRLPQIFL"
     gene            complement(3843..>4334)
                     /locus_tag="VFW59_10755"
     CDS             complement(3843..>4334)
                     /locus_tag="VFW59_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5364737.1"
                     /translation="LAPDYGTIKWTDKAKIGYYAQDHTADFAEDKSMTEWMTYWRGPH
                     DDDQTVRATLGRLLFSGDQALKQVKVLSGGEKGRMLFGKLMLQRNNVLLMDEPTNHMD
                     MESIESLNTALLEYKGTVVFVSHDREFVSSLATRIIEISDKGVTDYHGTYEEYLRDQG
                     MAV"
BASE COUNT          806 a         1211 c         1384 g          933 t
ORIGIN      
        1 gatcgggaaa ttcaggatcg cgatgcgtgc atcgaattcg ttttcggcct cgaaggtgtt
       61 cttgccggtc agcaggcggt acagcaccgc acccagcgca tagatgtcgg cacgctcgtc
      121 gagcggctgc ccctccagtt gctcaggcga catataggcc aggctgcccg ccaccatatc
      181 gccgctgctg ccagtcggaa tggcgcaacc gaagtcggtc agcttggcct gcccgttggg
      241 catcaggatg atgttttcgg gtttcacgtc gcgatgtacc acgccttgtc cggcgatgta
      301 tcgcagcgcc tgagcgatct gttcgacgat ggagatcacc gtgttgattg gcagcagggt
      361 atcgctgtgc tgatgctgtt gcagcgatac gccccggata tattccatca ccagataggg
      421 gccgcttttt tcggtgaaat tggcggagag caggcgcacg atgttcttgt gatgcagctt
      481 gccgaccagc gcgacctcgt tggacatcag tctgcgatag ggctcggtct ggcaggtctt
      541 gcgcaactgc ttgattgcga ccgagacgaa gttaccctcc tcgatggcca gatagacatt
      601 cgagctggcc ccggtgccga tctgttcgac tatggagtaa ttgccgatgt gggagggagc
      661 ggaaggagcg gccatccaga gtatttagtg cctataacgg aaacgttcga cccgcgcggg
      721 ctgaaaagat tccgtattga agcaaggtgg cttactgtaa ggcaaacact tccactcggt
      781 tgcgtccccg ctgcttggcg tggtagagcg cctgatccgc tcctgcgcat ccgtctggaa
      841 gcagcgagag gtgctcctcg atcacttggg cgatcaactt gccctcgagt atccgatgct
      901 gctgatggct ttgaatattg ggctgcagca atctttcacc ggcctccaga tttcgtacca
      961 gactgtgttc cagcatgtac tcgacgcttt cgaagtccat gccattgaac agggcgtttt
     1021 gttcgatcag ggcgcggtcg gttgatttca tggtcacttt atcaatgcct caaagttgcg
     1081 gttctggccg gagccggatt tagcggaaca caatggtctt gttgccgcat accagcaccc
     1141 gatcttccac atgatagcgc aggccgcgcg acagcacggt cttctcgatg tcccgcccgt
     1201 aacgcaccag atcatctacc gtatcgccgt gatcgacccg taccgtgtcc tgttcgatga
     1261 tgggcccggc gtccagctcg tcggtcacga aatggcaagt cgcgccgatc agcttcaccc
     1321 ctcggtggaa agcctgatgg tagggcttcg cgcccacgaa cgagggcaga aagctgtggt
     1381 ggatgttgat gatgcgtccc ggaaagcgct ggcacaggaa gggcggcaga atctgcatga
     1441 agcgcgccag caccagacaa tcgccgcgat gctgttcgaa cagcgccgcg atgtgttcga
     1501 acgcggtggc cttggcggct ggatcgttgg cgggcatgtc cacatggatg aaggggatgc
     1561 cgtgccattc gacgaaactg cgcaaatccc cgtgattgga gatcacgcag ggaatatcca
     1621 ccagcaactc gccgctgcgc cagcggtgca gcagatcgtt gaggcagtga tcctgtttgc
     1681 tcaccagtat caccagccgt tttttgatgg ccgaatcgga tagctgccag tccatggcga
     1741 actctttggc taaagtgccg aagcggtttt taaactcatg cgcatcgaac gggagcgaat
     1801 cggcccgcac ctcctgccgc atgaagaagc gctgcgtctc ctgatcggtg tgatagctgg
     1861 cctcgacgat aaacccgccg tgctgcgcga tgaagccgct caccgccgcg atgatgcccg
     1921 cccggtcggg gcaggagatg gtcagggtgt agtggtgggg catgcggtta tcctttgatg
     1981 tggcgcgtag attttaaagg ataacccgcg cgttgcggca agcttggcaa agcttgccgc
     2041 catgcccgcg tttcaggccg ccatttgtat ggcggtaatc tcgtcgaact cgaaaggaat
     2101 tgccgggagc ttgatctgga aggtcacgcc tttgccattg aggccgtcga gtatctcgat
     2161 gctgccgtgg tgcatggcga tggtctgatg ggcgttgaac aggccgaatc cgccgccctg
     2221 aaaactgcgg gcgttgttgg aggcctgcac gaacttgccg aacacgccgg cgcgttcgct
     2281 gaccgggatg ccggggccgt cgtccatcac cgatatcagc acgttgttgt cggcatcgct
     2341 gtcgatcagc accttcaccg tgccgccgtg cccgagaaac ttgagcgcgt tggagatcag
     2401 gttgttgacc gcgaagaagc ggatcttctg gatgtcccag tacacatatt ccggcaacgt
     2461 tccgatggtg acggagaagt cgatgttctt gccgagcgcc tcgttgtgga aggtcctttc
     2521 catgttgcgg gtgcgctcga tgagttcggc ccggcccagc agggcgggat ctgccgtttc
     2581 cttgccggca tgcatgcggg cggtggtgag catgttgttc accagtgcga gcatgtcgga
     2641 acccgccatt tcggcgatcg acaagttgcg cagcatcgca tcccggtcca gattctcgga
     2701 agcgatatgc atcttgagca tgtcgatggt gcagagtatg gcattgaggg gggacttcag
     2761 gtcatgggtc accgaagcca gaaaatctat ttcattgttg cgtgcatcca tggcgttgtc
     2821 tccgataggc atttgggcca agtatcatgg tcgacgatga cactgtgatg acaaggcatg
     2881 ggtaaccatg caactagtgt gccaatcttg agcccggttc ggtagcgtaa cttattgatt
     2941 cctgtggggc ggtgatgcac caatgcggtg ccggagtggg ccccggggtg caaagtggtg
     3001 cgcagggcca gcagcgaact acagaaaaat ctggggcagg cgctgtgcgg ttactgtctc
     3061 gccccataac agcgacatga tgtccgaggc ttgcgcagca tcgccgctgg tgaagaactg
     3121 cacgctggca tccgcttggg tgcgcccggt cagttcggtc tggatgcgcc gttgcaagtg
     3181 gcgtgcgacg gcttcgccgg tatcgatcaa ggcgatgctg tcgcctgcga tctcgcggat
     3241 cagcggggcg aggaagggat aatgggtgca gccgaggatc agcgtatcgg caccgcaagc
     3301 cagcagcggt gcggtgtaac gttcgagcag cgcgcgggtc tcggggccgt cgaggtcgcc
     3361 gcgttcgatc tgctcgacca gtccggggca gccctgagtg acgatctcga cttcgcccgc
     3421 atatttctcc agcaaggcgg caaagcgtgc gctttccagc gtgccgatgg tcgccagcac
     3481 gccgaccacg ccgctgcgag tggcggcgac ggcaggtttg acggcaggct ccatcgccag
     3541 gatgggcagg tcgaactggc tgcgtagcgt tgcggctgcg gcagcggtgg cggtgttgca
     3601 ggcgatgacg atggcgtcgg cgccctgttc gatcaggaag cgggtgagca ggagcgagcg
     3661 ctgctcgatg taggacgcgg gtttgtcgcc gtagggtaca tggccggaat cggcgacata
     3721 gatcaggcgc tcatgcggca gcgtacggcg gatgtgatgc agcacggaca gcccgcccac
     3781 gccggaatcg aatacgccta tggcacctga atgaaggctc acaccgtacg ataccgtagc
     3841 cgttaaaccg ccatgccctg atcgcgcaga tactcttcgt aggtgccgtg gtaatcggtc
     3901 acgcccttgt cggagatctc gatgatgcgg gtcgccagcg aggacacgaa ttcgcggtca
     3961 tggctgacga acaccaccgt gcccttgtat tccagcagcg cggtgttcag cgactcgatg
     4021 gattccatgt ccatgtggtt ggtcggctcg tccatcagca gcacgttgtt cctttgcagc
     4081 atcagcttgc cgaacagcat gcggcccttc tcaccgcctg acagcacttt cacctgcttg
     4141 agcgcctgat cgccggagaa cagcaaccgg cccagtgtgg cgcgcacggt ctgatcgtcg
     4201 tcgtgcgggc cgcgccagta ggtcatccac tcggtcatgc tcttgtcttc ggcgaagtct
     4261 gcggtatggt cctgagcgta gtagccgatc ttggccttgt cggtccattt gatggtgccg
     4321 tagtccggcg cgag
//
LOCUS       DASUIV010000299         8733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_77940, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000299 DASUIV010000000
VERSION     DASUIV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77940"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFW59_10760"
     CDS             <1..561
                     /locus_tag="VFW59_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364738.1"
                     /translation="NTTVDLRVFSDLLSKGMFPHTGGTFVNGLSAEYRGIEATLKHSF
                     DEVSELTLNLAHGAASSNGASMAAAGFTGLGSSCVGNNDILACSIPKDSASALYSRQF
                     SGEISVSAAYYYQASMQPFDRGIVDLQPTQHRTDVRIAKALRDVGGLRGDVALVVQNL
                     FDQTYTDYVANSLNNRRAYATLTVNW"
     gene            565..1485
                     /locus_tag="VFW59_10765"
     CDS             565..1485
                     /locus_tag="VFW59_10765"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX5364739.1"
                     /translation="MAAPCAAFVRAVLLLLCLVPLVSSAEALRVLVVLSDAAAPYQAF
                     AKTFRQNLPVSIKTSLIERAEDFSGDEGGADLIVTVGVKASYWVTQRTTTPVLAALIP
                     SSRYAELLAKHPRTSQLSAIYLDQPWARQAGLLRAALPERRNIGVLYSADTKLDLRAL
                     KEALSRQGFTLIAKLLKNNDTLFADLEEVLENSDVLLAIPDGTIYSSNHIRNILLSSY
                     RRGIPLMGSSQAYVNAGALCAIFSTPEHLAAQASVATLVFADTHRLPEAKYPELYSIA
                     VNREVGRTLNITIKSTELLQFQVEKTQGNL"
     gene            1485..2855
                     /locus_tag="VFW59_10770"
     CDS             1485..2855
                     /locus_tag="VFW59_10770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5364740.1"
                     /translation="MRKNKYGIEQHVAWLMLAPLLVMVIGLEAYFLHDRFADMNRGLL
                     MRGQLITHQLAASSEYGVFSNNQTFLNHTAESVLQQPDVRAVIIMNSASEILVAAGAV
                     PRALGAGASLSAIRPEPLLGLVNAEMRTLDQAGTVLLYQPILSTQVALDEIETPPAIH
                     AAGAVIVEMSWDQTNKLKSSLFWFTTFATAGLLVITLYLIHLASRRIVGPIRELSKAV
                     HAIGNGELDTRVAETSRIKELCTLTRGINQMTADLQNERAILQHRIDEATKQLRDLAF
                     YDTLTQLPNRRLLEDRLTQAFFVSKRNGCYGALMFLDLDKFKPLNDQFGHVVGDLLLV
                     EVAHRIRGCMREMDTVARFGGDEFVVLLSELSADRMEALKQAHILAEKIRSALEATYY
                     LIDSSSGSGERRIEHRCAASIGVAMFRDGEGSEEDAMRWADTAMYQAKQEGRNRVCFH
                     EPAPAI"
     gene            3057..5510
                     /locus_tag="VFW59_10775"
     CDS             3057..5510
                     /locus_tag="VFW59_10775"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX5364741.1"
                     /translation="MPALGMDADSIVDDFRHYFSHTLGRDEPEFSSYQMYESMVLSLR
                     DRLVERWKNTRRAYREADCKRTYYLSLEFLMGRALGNAMLNLNVDPATATALRKLEVE
                     LDAIAEHEHDAGLGNGGLGRLAACFLDSCASLQLPVMGYGIRYEYGMFRQKIEYGHQI
                     EEPDHWLRDGNPWEIERPEFATRIKFGGHTEYVTDRKGRTHVRWADTRDVMAMPYDVP
                     VPGYQNGTVNTLRLWSAAATEEFDLAEFNAGSYTEAVASKNAAEHITMVLYPNDASEN
                     GKELRLRQQYFLASASLQDVLRQWVMLRGQDFSQFADKNCFQLNDTHPTCAVPELMRL
                     LMDEHGLEWDEAWSITGRTMAYTNHTLLPEALEKWSVSMYGRLLPRLMEIIREINVRF
                     LTQVALHYPGDIERQRRMSIIEEGHEPQVRMAYLAVVACHSVNGVAALHSELLQKNLF
                     RDFYEMWPQKFNNKTNGVTPRRWLAWSNPALSELIDQSVGDSWRTDLSELQQLKKFAN
                     DPAFRTRWYEVKQVNKQRLADMVKHDCGVDFDVNAMFDVQVKRIHEYKRQLLNVLHVI
                     HLYDRIKRGDTANWTSRCVLIGGKAAPGYMMAKRIIKLVNSVAHVINSDPDTEGLLRL
                     AFLPNYRVTAMEVICPGTDLSEQISTAGKEASGTGNMKFMMNGALTIGTLDGANIEIR
                     EEAGEENFFLFGLTAEQVDATRGHYDPAAIINADEDFRRVMGLLTSGHFNLFEPGIFD
                     PIIDSIYNPADPWLTAADFRSYVDSQHKVDMAYKNRDEWTRMSIINTATSGKFSSDRT
                     ILDYNREVWGLPQVSPTLP"
     gene            complement(5669..8164)
                     /locus_tag="VFW59_10780"
     CDS             complement(5669..8164)
                     /locus_tag="VFW59_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5364742.1"
                     /translation="MKRMLFNATQPEELRVAIVEGQKLIDLDIETAGKEQRKSNIYKA
                     VITRIEPSLEACFVEYGGSRHGFLPFKEVSPQYHQPGCGNRPSIKEALREGQELLVQV
                     EKDERGNKGAALTTYISLAGRYIVLMPNNPNGGGVSRRIEGEERNELREVLSHVEVPQ
                     GMSIIARTAGIGRTEEELQWDLNYLKQLWDAIDGAAASEKAPSLIYLEGSLVVRAIRD
                     YFNPEIGEILIDTEEIYQQALAFMSTVMPDNVNRIKRYVDDVPLFSRFQIEHQIESAH
                     AREVRLPSGGAIVIDHTEALTSIDINSARSTKGADIESTAFNTNLEAAEEIARQLRLR
                     DLGGLIVIDFIDMENSKNQRDVENRLRDSLRYDRARVQTAKISRFGLLELSRQRLQPS
                     LGESNYIACPRCSGIGHIRGTESSALHILRIIQEEAMKDSSVAIHAQVPVDVATFLLN
                     EKRGDIHRIESRLKVSVTLIPNPHMETPNYTINRLRHDDLTAEVMQASYMLVEKPTED
                     KQLTQGQEQKKAVRATAVVQGITPSQPAPVKVEEAKPQPSLISKVVGWFKSLTAEEET
                     PKAKPALAKSRPRRERGERGARSERGEGGESGKRRERGEDGEGGKRRERGEGGEGGKR
                     RERGEKPAPAEGKEPRQQGEREPRPPKQPRPPRIAPAQQAETEKPVLESQIVAAEQTE
                     EGSAREGARRRGRRGGRRERERRENTTPNGESPTALKPSEYVAYPEGYVHPMKPTEYI
                     AYPEGYVAPVKPSEYIAYPAVAPRAPRTPQPVSTPTGGEGLVQIETDPGKFNATGEAA
                     NAGQSAPRRRQRTREVYVESEPLVQIETQHPQA"
     gene            8523..>8733
                     /locus_tag="VFW59_10785"
     CDS             8523..>8733
                     /locus_tag="VFW59_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX5364743.1"
                     /translation="MNGLSKDSVSFIEIDESGETQRIDNFLFRHLKGVPKSHVYRILR
                     GGEVRVNKKRVTQTYRLQLGDVLRIP"
BASE COUNT         1725 a         2368 c         2626 g         2014 t
ORIGIN      
        1 aatacgacag tcgacttgcg cgtgtttagc gacttgctca gcaaagggat gtttccgcat
       61 accggaggga catttgttaa tggactctct gccgaatatc gcgggataga ggccacgctg
      121 aagcattcat tcgatgaggt cagtgagctc acccttaatc tggcccacgg tgcggcaagc
      181 agcaatggcg cgtctatggc cgctgccgga ttcaccggcc tcggttctag ttgcgtcgga
      241 aacaatgata ttcttgcttg cagtatcccg aaggacagtg cgagcgcgct atattcccgg
      301 cagttttcgg gtgagatttc tgtgagtgcc gcctattatt atcaggcgag catgcagcca
      361 ttcgaccgtg gcatagttga tcttcagccg acccagcatc gcaccgatgt gcgtatcgcc
      421 aaggcgcttc gggacgtggg cgggttacgg ggagatgttg cgctggtcgt gcagaatctg
      481 ttcgaccaga cctataccga ttacgttgcc aacagcctca acaatcggcg tgcctatgcc
      541 acgctgacag tgaactggta gggaatggcc gctccttgcg ccgcatttgt cagggcggta
      601 cttctgctgc tgtgtctggt gcctttggtc agctcggcag aagcgctgcg tgtgctggtc
      661 gtgctgagcg atgcggccgc gccgtatcag gccttcgcaa agacattccg gcagaacctc
      721 cctgtcagta tcaagacctc gcttattgag cgtgctgaag atttctctgg cgatgaaggc
      781 ggcgcggatc tcatcgtgac cgtcggggtc aaagcctcat attgggtgac acagagaacg
      841 acgactccgg ttctggctgc gctcattccg agtagtcgct atgccgaatt gctggccaag
      901 catccacgaa ccagtcaact ttccgcgatc tacctcgatc agccttgggc aaggcaggcg
      961 gggctgttgc gcgccgctct gccggagcgc agaaatatcg gcgtgctcta ttctgccgat
     1021 acaaaactgg atctgcgggc attgaaagag gcattgagtc gccagggttt cacgctgatc
     1081 gccaagctgc tgaagaacaa cgacaccctg tttgccgatc tggaagaggt attggaaaat
     1141 agcgacgttt tgctggccat acctgacggc accatctaca gcagtaacca tattcgcaat
     1201 atcctgctga gcagttatcg gcgcggtatt cctctgatgg gctcgtcgca ggcttacgtc
     1261 aatgccggtg cactgtgcgc gatcttttcc acgccggaac atttagcggc acaagccagt
     1321 gtcgccaccc ttgtgtttgc ggatacccat cgtttgcccg aggctaagta tcctgagctc
     1381 tatagtattg cggtcaatcg ggaggtgggc aggaccctga atatcacgat caaatcaaca
     1441 gagctgttgc agttccaggt tgagaaaaca caaggtaatt tgtgatgcgg aaaaataaat
     1501 acggcataga gcagcatgtc gcctggttaa tgctggcacc tttgctggtg atggtaatcg
     1561 ggctggaagc ctacttcttg catgatcgtt tcgcagatat gaatcgcggc ctgctcatgc
     1621 gcggccaatt gatcactcat caattggctg ccagcagtga gtatggggtg ttttcgaata
     1681 atcagacgtt cctgaaccat accgctgaaa gtgtgttgca gcaaccggat gtgagagcgg
     1741 tgatcataat gaattccgct tccgagattc tggtcgcggc aggtgcggtg cctcgtgcct
     1801 tgggcgcagg cgcatccctg tcagcgatac ggcctgagcc gttgctgggc ttggtgaatg
     1861 ccgagatgag gacgctggat caggcaggga cggtgctgct gtaccagcct atcctgtcga
     1921 cacaagtcgc gctggatgag atcgaaacgc ccccggctat tcatgcagcg ggagccgtga
     1981 ttgtggaaat gagctgggat caaacgaata aactgaaatc cagtttgttc tggtttacga
     2041 catttgccac tgccgggttg ttggtgatta ccttgtatct gatccatctg gccagtcgac
     2101 gcatcgttgg ccccatcaga gagttgagca aagcggtgca tgccatcggc aatggggagt
     2161 tggacacccg tgtggcggaa acgagtcgta tcaaagagtt gtgtactttg actcggggca
     2221 tcaatcagat gacggctgat ctgcagaacg agcgggcaat tttgcagcac cgtatcgatg
     2281 aggcgaccaa gcagttacga gatttggcat tttatgacac attgacgcag ttgcccaatc
     2341 gccgcctgct ggaagaccgg cttactcagg cattttttgt cagcaagcgc aacggttgtt
     2401 acggcgcgtt gatgtttctc gatctggata aattcaaacc gctcaatgac caattcggtc
     2461 acgtagtcgg cgatctgttg ctggtcgaag tcgcccatcg tatccgtggt tgcatgcggg
     2521 aaatggatac ggttgcccgt tttggtgggg atgaattcgt ggtgctgctc agcgagttga
     2581 gtgcagaccg tatggaggct ctcaagcagg cgcatatcct tgcggaaaag atacgctcgg
     2641 cattggaagc gacctactat ctgatcgatt cctcgtctgg gagcggggag cgaaggatcg
     2701 aacaccgttg tgccgccagt atcggtgtgg caatgttccg ggacggtgag ggtagcgagg
     2761 aagatgccat gaggtgggcg gatacggcga tgtatcaggc taaacaggaa gggcgcaatc
     2821 gggtgtgttt tcatgaacct gcccctgcca tctgagctca gtcaggcccg tccgcgtatt
     2881 ttttgacgat gcgggtataa ttcgccgccc tgattgatat gttagaggtt gctcgaaacc
     2941 tattctcgga aaattcgctt gtcggcctga gccgtatcgc ctgtatggat aaaccctagg
     3001 agtcaattat caaagcccca tccaaaaaga atcgccagcc ttccctgatc ggcaacttgc
     3061 ctgcgctcgg catggatgcc gacagcatcg ttgacgattt ccgccattat ttcagccata
     3121 ctctggggcg cgacgagcca gagttctcca gctaccagat gtatgaatcg atggtgctgt
     3181 cgctgcgcga ccgtctggtg gagcgctgga agaacacgcg gcgggcttac cgcgaggcgg
     3241 actgcaaacg cacctattat ctgtcgctcg aatttctgat ggggcgcgcg ctgggcaatg
     3301 ccatgctgaa tctcaacgtc gatcccgcca cggccacggc tctgcgcaag ctggaagtgg
     3361 aactcgacgc gatcgccgag cacgaacacg acgcgggttt ggggaacggc ggcctcgggc
     3421 gtctggcggc ctgtttcctc gacagttgcg cttcgttgca gctgccggtg atgggctacg
     3481 gcatccgcta cgaatacggc atgttccgtc agaagatcga gtatggtcac cagatcgagg
     3541 aaccggacca ctggctgcgc gacggcaacc cctgggagat cgagcgccct gaatttgcca
     3601 cgcgaatcaa gttcggcggt cacacggaat atgtcaccga ccgtaagggc agaacccatg
     3661 tgcgctgggc agatacgcgc gacgtgatgg cgatgcccta tgatgtgccg gtaccgggct
     3721 accagaacgg tacggtgaat acgctgcgcc tgtggagtgc ggcggcaacc gaagaattcg
     3781 atctcgccga attcaatgcc ggcagttata ccgaggcggt cgcctcgaaa aatgccgcag
     3841 agcacatcac catggtgctc tatccgaacg atgcaagcga gaacggtaaa gaactgcgtt
     3901 tgcgtcagca atatttcctc gcctcggcca gcctgcagga tgtactgcgt cagtgggtca
     3961 tgctgcgagg tcaggatttc agccagttcg ccgacaagaa ctgtttccag ctgaacgata
     4021 cccatccgac ctgtgcggtg ccggaactga tgcgcctgct gatggatgaa cacggtctgg
     4081 aatgggacga ggcatggtcg atcaccgggc gcaccatggc ctacaccaat cacactctgt
     4141 tgcccgaggc gctggagaag tggtccgtca gcatgtacgg ccgcctgctg ccgcgtctga
     4201 tggaaatcat ccgcgagatc aatgtgcgtt ttctgacgca ggtggcactg cactatcccg
     4261 gcgatatcga gcgtcagcgc cgcatgtcca tcatcgaaga ggggcatgag ccgcaggtgc
     4321 gcatggctta tctggctgtt gttgcctgcc attcggtcaa cggcgtcgcc gcgctgcatt
     4381 ctgagttgct gcagaaaaac ctgttccgcg atttttacga gatgtggccg cagaagttca
     4441 ataataagac caatggggtg acgccgcgtc gctggctggc ctggagcaac ccggcactga
     4501 gcgaactgat cgatcagtcc gtcggcgata gctggcgcac cgatctgtcc gagttgcagc
     4561 agctgaaaaa attcgcgaat gatcctgcgt tccgcacccg gtggtacgag gtgaagcagg
     4621 tgaacaaaca gcgtctggcc gacatggtca aacacgactg tggcgttgat ttcgatgtca
     4681 atgcgatgtt cgatgttcag gtcaaacgca tccacgaata caagcgccag ttgttgaacg
     4741 tgttgcatgt catccatctc tatgaccgca tcaaacgcgg cgacacggcc aactggacat
     4801 cacgctgtgt gctgatcggc ggcaaggccg cacctggcta tatgatggcg aagcgcatca
     4861 tcaagctggt gaactcggtg gcccatgtga tcaacagcga cccggatacc gagggcttgc
     4921 tgcgactggc attcctgccc aattaccgtg tcaccgcgat ggaggtgatc tgtcccggca
     4981 ccgatttgtc ggaacagatt tccacagcag gcaaggaagc ctcgggcacc ggcaacatga
     5041 agttcatgat gaacggtgcg ctgaccatcg gcacactgga tggcgccaac atcgaaatcc
     5101 gcgaagaggc tggcgaggaa aacttcttcc tgttcgggct gaccgcagaa caagtagacg
     5161 cgactcgcgg tcattatgac ccggcagcga tcattaacgc cgacgaggat tttcgtcgtg
     5221 tgatgggatt gttgaccagc ggacacttca atctgttcga gccgggtatt ttcgacccca
     5281 tcatcgattc gatctacaac cctgctgatc catggctgac cgcagctgac ttccgcagct
     5341 atgtggatag ccagcataag gtggatatgg cttacaagaa tcgcgacgaa tggacgcgca
     5401 tgagcatcat caacacggcg accagcggca agttctccag tgatcgaacc atcctcgatt
     5461 acaaccgcga agtctggggg ttgccgcagg tatcaccgac gctgccttga cctgtccccc
     5521 cagggagcgg agctccttgg gaacagacat ggtggctgga tattgaataa gggcttcgat
     5581 ttcgaagccc ttatttcatg gtgcgacata aacagaaagg ccgcccgatt ctcatcgggc
     5641 ggcctttctg ttttattgcc tgagcgcgtt acgcttgcgg gtgttgtgtc tcgatttgaa
     5701 ccagtggttc gctctctaca tacacttcgc gtgtgcgttg acgtcgcctt ggggcagatt
     5761 ggccagcatt cgcggcttca ccggttgcgt tgaacttgcc cggatcggtc tcgatctgga
     5821 ccagcccttc gccgccggtt ggagtcgaaa ccggctgcgg agtcctgggc gcacgggggg
     5881 caacagccgg ataagcgatg tactcgctcg gttttaccgg tgcgacataa ccttctggat
     5941 aggcgatgta ctcggtcggc ttcatgggat gcacgtagcc ttccgggtag gccacatatt
     6001 cgctgggctt caacgcggtc ggggactcac cgtttggcgt ggtgttttca cgacgttcgc
     6061 gttcgcgacg accgccgcga cgaccgcgac ggcgagcacc ttcacgggcg ctgccttctt
     6121 ctgtctgttc ggcagcgacg atctggcttt ccagtaccgg cttttctgtt tcggcctgct
     6181 gcgcaggtgc gatgcgtggt ggacggggtt gtttgggtgg acgtggttcg cgttcacctt
     6241 gctgacgagg ctctttgcct tcggctggcg ccggtttttc accgcgttca cggcgcttgc
     6301 cgccttcgcc gccttcaccg cgctcacggc gcttgccgcc ttcgccgtct tcgccgcgct
     6361 cacggcgttt gccgctttcg ccaccttcgc cgcgctcact acgtgcaccg cgctcaccgc
     6421 gctcacggcg tgggcgagat ttggcgaggg ctggtttggc tttcggtgtt tcctcttctg
     6481 ccgtcagact cttgaaccag cctactacct tgctgatcaa cgagggttgg ggtttggctt
     6541 cttccacttt gaccggtgct ggctgtgacg gggtgatgcc ttgcaccacg gctgtcgcac
     6601 gcacggcttt cttctgctcc tgtccctgag tcagctgttt gtcctcggtc ggcttttcca
     6661 ccagcatgta actggcttgc atcacttcgg cggtcaagtc atcgtgacgc agtcggttga
     6721 tggtgtagtt aggcgtttcc atgtgcggat tggggatcag cgtgacgctg accttgaggc
     6781 gcgattcgat gcgatggatg tcgccgcgtt tctcgttgag caggaaggtg gcgacatcca
     6841 cgggtacctg agcgtggatg gcaacgctgc tgtccttcat cgcctcttcc tgaatgatgc
     6901 gcaggatgtg cagcgcggag gactcggtac cgcggatatg gccgatgccg ctgcagcgcg
     6961 ggcaagcgat atagttgctc tcgcccaggc taggctgcag gcgttgacgc gacagttcca
     7021 gcaggccgaa gcgcgagatc ttcgcggtct gcacgcgggc acggtcgtag cgcagcgagt
     7081 cgcgcaggcg attttcgaca tcgcgctggt tcttgctgtt ttccatgtcg atgaagtcga
     7141 tcacgatcag accgcccagg tcgcgcaggc gcaactggcg ggcgatctct tccgccgctt
     7201 cgagattggt attgaaagcg gtcgattcga tgtccgcgcc cttggtcgag cgcgccgagt
     7261 tgatgtcgat ggaggtcagg gcttcggtat ggtcgatcac gattgcgccg ccggaaggca
     7321 gacgcacttc acgcgcatgg gccgactcga tctggtgttc gatctggaag cgcgagaaca
     7381 gcggcacatc gtccacgtag cgcttgatgc ggttgacgtt gtccggcatc acggtgctca
     7441 tgaacgccag cgcctgctga taaatttcct cggtgtcgat caggatttcg ccgatctccg
     7501 ggttgaagta gtcgcgaatc gcgcgcacga ccaggctgcc ttccagatag atcagcgagg
     7561 gggccttttc ggaagcggcg gcgccatcga tggcgtccca cagctgcttg aggtaattca
     7621 gatcccactg cagctcttct tcggtgcgcc cgatgcctgc ggtgcgggcg atgatgctca
     7681 tgccctgcgg tacttcgaca tgcgacagca cttcgcgcaa ctcgttgcgc tcttcgccct
     7741 cgatacggcg cgatacgccg ccgccattcg ggttgttcgg catcagcacg atgtagcggc
     7801 cggccagact gatgtaggtg gtcagtgccg cgcccttgtt gccgcgctcg tccttctcga
     7861 cttgcaccag cagttcctgg ccttcgcgca acgcctcctt gatggagggg cgattgccgc
     7921 agccgggttg gtgatattgg ggggagactt ccttgaaggg gaggaacccg tgacgactgc
     7981 cgccgtattc gacgaaacag gcttctaggc tgggctcgat gcgggtgatg actgccttgt
     8041 agatgttgct cttgcgttgc tctttaccgg cggtttcaat gtccaggtcg atcagtttct
     8101 gaccttcgac aatggcgacg cggagttctt ccggctgcgt cgcattgaat aacatgcgtt
     8161 tcatttttta ttgtctcccg cgctctgaca cgggcgagac ccagctacac gcaattaggc
     8221 gtgtgagagt agcaatcgtg caaacagttc ggagttatac gtacgtttga tcaaaatcgg
     8281 ttctcggtcg gtagcttgtc ttaatttgtt ccgggtgacg cagcttgatg cgcgtcacta
     8341 aaaatccaca ggtgctttga gcgcgtattt caattaccat cactgcttgt tttccggtga
     8401 gtgacattaa ccgggcgggc ggctgcttca tgggtggctt gactgcctac ctcaacatgc
     8461 gacgggggat taacaacccg ttttcacgaa ggcgcacacg cgagcgacga agtatattca
     8521 aaatgaatgg tttaagcaaa gattctgtct cctttataga gatagacgag agcggcgaaa
     8581 cgcagcgcat cgacaacttc ctgttccgcc atctgaaagg cgtgccgaag agccacgtct
     8641 accggatttt gcggggcggc gaggtgcggg tgaacaagaa gcgggtgacg cagacctatc
     8701 gtctgcaatt gggcgatgtg ctgcgcattc cgc
//
LOCUS       DASUIV010000300         3790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_545325, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000300 DASUIV010000000
VERSION     DASUIV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545325"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..2214)
                     /locus_tag="VFW59_10790"
     CDS             complement(196..2214)
                     /locus_tag="VFW59_10790"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5364744.1"
                     /translation="MSTGLGFALLCAIVALLYGVFSIRWILSKPTGNDRMREIAAAIQ
                     EGASAYLSRQYATIAMVGAILFIAIFFALGWQTAFGFAIGAVLSGAAGFIGMNVSVRT
                     NVRTAAAASDGLNAALNIAFRGGSITGMLVVGLGLLGVAGYYVILTGIGASPAEATHA
                     LVGLGFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDN
                     VGDCAGMAADLFETYAVTIIATMLLGGLLMADAVPNAVIYPLVLGGFSIIASIAGTFF
                     VKARPGGKIMNALYRGLAVSAGLALVAFYPLTAWMMGDNGTYSVNALYGSAVIGLLLT
                     ATLVWITEYYTATEYGPVRHIAQASTTGHGTNVIAGLSVSMRATAAPVLSVCLAIWSA
                     HELAGLYGIAIAATSMLSMTGIIVAIDAYGPITDNAGGIAEMAALPKEIRDITDPLDA
                     VGNTTKAVTKGYAIGSAGLAALVLFADYTHALTAGGKMLTFDLSNHMVIIGLFIGGMV
                     PYLFASMAMEAVGRAAGSVVVEVRRQFKEIPGIMEGTGKPEYDTCVDMLTRAAIREMI
                     VPSLLPVLVPILVGKFLGAQALGGLLIGTIVTGLFVAISMTVGGGAWDNAKKHIEEGH
                     HGGKGSDAHKASVTGDTVGDPYKDTAGPAINPLIKIINIVALLIIPML"
     gene            2767..>3790
                     /locus_tag="VFW59_10795"
     CDS             2767..>3790
                     /locus_tag="VFW59_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEX5364745.1"
                     /translation="MFKNMKVRVQLGLGFGLALLLMVAIALTGIFGMKASNDQLDDIV
                     NDNMRKIELVNTMSESVHIVSRVLRSMVLLDDATAIKEEGQKLVVARAAYDKAWSELQ
                     KMPASERGQAIRAKISELSAIARPINNKIFELALANQDDEARALLMKEGRPAVNKWQS
                     ALDENSALQKEITDKQAVEAAADYSRASTIMIALSVLGIVLSLFIAFTITRNLMSQLG
                     GEPAQTAEISNKIATGDLSAKIELKSGDTTSLSYSMSVMQASLTQIVGEIRNIVAAAN
                     KGDFSTKMDLNGKAGYTKELSELLNQLSDTVDTAFKDTIHVAQALENGDLTQTVTREY
                     QGAFDLV"
BASE COUNT          789 a         1110 c         1152 g          739 t
ORIGIN      
        1 aaaattcaaa ggcttgcgaa ttttccgggg ttcaccctgt gggtgaacac atcgcctcac
       61 gcaatgcctt gtttttcctc tgtgttctct gcgcctctgt ggtgtaattg cgtttttaag
      121 gatcatggat acggagaccc ccgaaataaa aagcctgccg gaaacaaccg gcaggctttt
      181 tgacttctct cgcgattaca gcatcgggat aatcagcagc gccacgatgt tgatgatctt
      241 gataagcggg ttgatcgccg ggccggcggt gtccttgtag gggtcaccca cggtgtcgcc
      301 ggtcaccgat gccttgtgcg catcgctgcc cttgccgccg tggtggcctt cttcgatgtg
      361 cttcttggcg ttatcccacg caccgccgcc caccgtcatc gagatggcga cgaacaggcc
      421 ggtgacgatg gtgccgatga gcaggccgcc caaggcctgc gcaccgagga acttgccgac
      481 caggatgggc accagcaccg gcagcagcga cggcacgatc atttcgcgga tcgccgcacg
      541 ggtcagcatg tccacgcagg tgtcgtattc cggtttgccg gtgccttcca tgatgccggg
      601 gatttccttg aactggcggc gcacttcgac cacgacgctg cccgccgcgc gaccgaccgc
      661 ttccatcgcc atcgatgcga acaggtaggg caccatgccg ccgatgaaca ggccgatgat
      721 gaccatgtgg ttggacaggt cgaaggtcag cattttgccg cccgcagtca gcgcgtgggt
      781 atagtcggcg aacagcacca acgccgccag cccggcggag ccgatggcat aacctttggt
      841 gacggccttg gtggtgttgc cgaccgcgtc gagcgggtcg gtgatgtcgc gtatctcttt
      901 gggcaacgcg gccatttcgg cgatgccgcc cgcgttgtcg gtgatcgggc cgtatgcgtc
      961 gattgcgacg atgatgccgg tcatcgacag catcgaggtc gcggcgatgg cgataccgta
     1021 cagcccggcg agttcatgtg cgctccagat ggccaggcac accgagagca ccggggcggc
     1081 ggtggcgcgc atcgacacgc tcaggcccgc gatgacgttg gtgccgtgcc cggtcgtgga
     1141 ggcctgagcg atgtggcgta ccgggccgta ttccgtggcg gtgtagtact cggtgatcca
     1201 caccagcgtg gcggtcagca gcaggccgat gacggcggag ccgtacagcg cgttgacgct
     1261 gtatgtgccg ttgtcgccca tcatccatgc ggtgagcggg tagaacgcga ccagcgccag
     1321 tccggctgag accgcgaggc cgcgatacag cgcgttcatg atcttgccgc cggggcgcgc
     1381 cttgacgaaa aacgttcccg cgatggaggc gatgatcgag aagccgccca gcaccagcgg
     1441 gtagatcacc gcgttcggca cggcgtcggc catcagcagg ccgcccagca gcatcgtcgc
     1501 gatgatggtc accgcatagg tttcgaacag gtcggcggcc atgcccgcgc agtcgccgac
     1561 gttatcgccg acgttgtcgg caatcactgc cgggttgcgc ggatcgtctt ccgggatgcc
     1621 cgcttcgacc ttgccgacca ggtccgcgcc gacatccgcg cccttggtga agatgccgcc
     1681 gcccagccgc gcgaagatcg agatcagcga gccgccgaag cccagtccca ccagcgcatg
     1741 cgtcgcttca gccggagatg cgccgatgcc ggtcagtatc acgtaatagc ccgcgacgcc
     1801 cagcaggccg agccccacca ccagcatgcc ggtgatcgag ccgcccctga acgcgatgtt
     1861 cagcgcggcg ttcagcccat cgctggccgc cgctgcggtg cgcacgttgg tgcgcaccga
     1921 aacgttcatg ccgatgaatc cggctgcacc cgagagcacc gcgccgatcg caaaaccgaa
     1981 ggcggtttgc cagccgagcg cgaagaagat cgcaatgaac aggattgcgc cgaccatcgc
     2041 gatggtggcg tattggcggc tcaggtaggc ggatgcgcct tcctggatgg ctgcggcaat
     2101 ctcgcgcatc cggtcgttgc cggtgggttt ggacaaaatc cagcggatgg aaaatacgcc
     2161 atacaagagc gcaacgatgg cgcacaacag tgcaaatcct agaccagtcg acatgatttt
     2221 ctcccttgcg ttgagttgca aaaatcgtct gttgcccgaa aaaacgtctg ttgcgtgatg
     2281 cctggacagc gtgtgctgaa cgtgcaaatg atagcagtat ccggtaactc ttatagcatc
     2341 gagtaaggct gggggaaagg gggggcacac agcaagcagc tcggatggaa tgcaaaggaa
     2401 tccggggctt tccggtaacc gtacgtcatc ccggattccg tttcgctcca tctgggctac
     2461 ggttttgcgg tcaagtcgct gtccggcgaa ggccggaatc cactttttgc gagttgggca
     2521 taaagctttc catccctgcg aactcagttt caactgacgt cacaccggcc ctctgacttg
     2581 gggatattcc gagtgatttt gtagggatag tcttaccccc gcgtcttttg cctacaaaaa
     2641 aatcaagatt tcctgatagt tctggtcagt gagatatgag atcctttctg tgtaggatta
     2701 atcctatatg caattttttt gacggtagtt gtcgtttgtc ggcaaggtaa gttttgagag
     2761 gtcatcatgt ttaaaaatat gaaagtccgg gtgcaactcg gtttggggtt tggtcttgcg
     2821 ttgctgttga tggtagccat cgcgctgacg ggcatattcg ggatgaaagc cagcaacgac
     2881 cagctagatg acatcgttaa cgacaacatg cgcaagatcg aactggtcaa caccatgtcc
     2941 gaatcggtgc atatcgtttc ccgcgtattg cgctccatgg tgctgctgga tgacgcgacc
     3001 gccatcaagg aagaagggca gaaactggtc gttgcgcgcg cagcttacga caaagcctgg
     3061 agtgagttgc aaaagatgcc cgcaagcgaa cgcggacaag ccatccgcgc caagatcagc
     3121 gagttgtcgg cgattgcgcg tccaatcaac aacaaaatct tcgagctggc cttggccaac
     3181 caagatgacg aggcgcgcgc cctgctgatg aaagaaggtc gtcccgctgt caataaatgg
     3241 cagagcgcgc tggacgagaa cagtgcactg caaaaggaaa tcaccgacaa gcaggcagtc
     3301 gaggccgctg cggactacag tcgcgcatcg acgatcatga tcgcgctctc cgtgctgggc
     3361 atcgtgctgt ccctgttcat cgccttcacg atcacgcgca acttgatgag ccagttgggc
     3421 ggcgagcccg cacagacggc tgaaatctcc aacaagatcg caacgggcga cctgagcgca
     3481 aaaatcgaac tcaagtccgg cgacaccacc agcctgagct actccatgag cgtgatgcag
     3541 gccagcctga cccagatcgt cggcgaaatc cgcaacattg tcgcagcggc gaacaagggc
     3601 gacttcagca ccaagatgga tttgaacggc aaggcaggct ataccaagga actgtccgaa
     3661 ctgctgaacc agttgtccga taccgtcgat accgcgttca aggacaccat ccatgtcgcg
     3721 caagcgctgg aaaacggcga cctgacccag accgtcaccc gcgagtatca gggcgcgttc
     3781 gacctggtca
//
LOCUS       DASUIV010000301         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_914471, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000301 DASUIV010000000
VERSION     DASUIV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914471"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /gene="dsbD"
                     /locus_tag="VFW59_10800"
     CDS             <1..1070
                     /gene="dsbD"
                     /locus_tag="VFW59_10800"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEX5364746.1"
                     /translation="AGVAAGFSGNLISNALQTPWVLGSFAAVFVVLSLSMFGFYELQL
                     PNALQSKLTDASNRLHGGHLSGVFVMGALSAVIMGPCVAAPLAGALLYIGQTHDAALG
                     GTALFALAMGMGAPLLLIGSSAGVLLPRAGAWMEAVKRFFGVLLLGLAIWIVSPVIPV
                     GVQMALWAALLVFSGIYLRALDPLPHHSSGWSRLVKGVGILALLLGVAYLIGALSGAR
                     DLLRPLGNIGRATVETPATLQFSRVASVAELDARLASAGGKLVMLDFYADWCVSCKEM
                     ERFTFSDPAVQARLKNAVVLQADVTANSEDDKALLKRFGLYGPPGILFFDAKGGEMND
                     LRVVGYQDAAQFLKTLQRAGL"
     gene            1101..1469
                     /locus_tag="VFW59_10805"
     CDS             1101..1469
                     /locus_tag="VFW59_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX5364747.1"
                     /translation="MSDLNDPRVFFAAERTLLAWNRTSLALMAFGFVIERFGLFVSIL
                     MPKHEMPLQRGLSFWTGLAFVVLGAAVAAYSTVQFRKVLKTLRPIEIPEGYRVSPGIV
                     TNLTVAALGIVLTAYFFMGV"
     gene            1549..1989
                     /gene="aroQ"
                     /locus_tag="VFW59_10810"
     CDS             1549..1989
                     /gene="aroQ"
                     /locus_tag="VFW59_10810"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5364748.1"
                     /translation="MKTILVLHGPNLNLLGTREPGVYGRVTLDEINQNLTALAAENGA
                     NLSCFQSNAESALVDRVQHARDDGTDFIVINPAAFTHTSVALRDALAAVAIPFIEVHL
                     SNVFAREAFRKESFFSDIAVGVISGLGASGYEFAVRYALNHKVS"
     gene            1999..2451
                     /gene="accB"
                     /locus_tag="VFW59_10815"
     CDS             1999..2451
                     /gene="accB"
                     /locus_tag="VFW59_10815"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5364749.1"
                     /translation="MDLRKLKTLIELVEGSGIAELEISEGEERVRITRTVAASQHIVA
                     APAPQVVAAAPVAAVAAAPAEPIKAAAPEGHIVKSPMVGTFYRAASPGAKAFVDVGQS
                     VNKGETLCIIEAMKLLNEIESDQSGVIKAILVENGQAVEFGQPLFVIG"
     gene            2510..>3386
                     /locus_tag="VFW59_10820"
     CDS             2510..>3386
                     /locus_tag="VFW59_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5364750.1"
                     /translation="MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADADAKYVKL
                     ADESVCIGPAASNQSYLSIPAIISAAEVTDAQAIHPGYGFLSENADFSERCEKSGFVF
                     IGPRADTIRLMGDKVSAKIAMKKAGVPCVPGVEGALPDNPAEIVKIARSIGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVTMTKTEAQAAFNNPMVYMEKFLENPRHIEFQILADQ
                     HGNAVYLGERDCSMQRRHQKVIEEAPAPHLNTRLRNKIGERCAEACRKIGYRGAGTFE
                     FLYENDEFYFIEMNTR"
BASE COUNT          620 a          967 c         1089 g          710 t
ORIGIN      
        1 cagccggtgt ggcggcgggc ttctccggta atctgatttc caatgccctg cagacgccct
       61 gggtgctggg cagcttcgcc gccgtgttcg tggtgctgtc gctctccatg ttcggcttct
      121 atgagctgca attgcccaac gcgctgcaaa gcaagctgac cgatgccagc aacaggctgc
      181 acggcggcca tctgtccggg gtgttcgtga tgggcgcgct gtcggccgtc atcatgggcc
      241 cctgcgtggc cgcgccgctg gcgggtgcgt tgctatatat aggtcagacc cacgatgcgg
      301 cgctgggcgg caccgccttg tttgcgctgg caatggggat gggcgcgccg ctgctgctga
      361 tcggcagctc ggcgggcgtg ttgctgccca gagcgggcgc gtggatggag gcggtcaaac
      421 gcttcttcgg cgtgctcctg ctggggttgg cgatctggat cgtctcgccg gtcatcccgg
      481 tcggggtcca gatggcgttg tgggcggcgc tgctggtctt ttccggcatc tatctgcggg
      541 cgctggatcc gctgccgcac cattccagcg gctggagccg actggtcaag ggggtgggca
      601 tcctcgcctt gctgctgggc gtagcttatc tgatcggtgc gctgtcgggc gcgcgcgacc
      661 tgttgcgacc tcttggcaat atcggacggg caacggtcga gacgcctgcc acgctgcaat
      721 tctcccgtgt cgccagtgtc gcggagctgg atgcgcgtct ggccagcgcg ggcggaaaat
      781 tggtgatgct ggatttttat gccgactggt gcgtctcatg caaggaaatg gagcgcttta
      841 ccttcagcga cccggcggtg caggcgaggc tgaagaatgc cgtggtgctg caggcggacg
      901 tgaccgcgaa tagcgaagac gataaggcgc tgctcaagcg ctttggtctg tatggtccgc
      961 ccggcatcct gttcttcgat gcaaaaggcg gcgagatgaa cgatcttcgc gtggtgggct
     1021 atcaggatgc cgcgcagttc ctgaagacgc tgcagcgtgc cgggctgtag gattcataac
     1081 ttgcctagag aaggaatgcc atgtcagatc tgaacgaccc cagagtgttc tttgccgccg
     1141 agcgcacctt gctggcatgg aaccgcacca gtctggccct gatggccttc ggcttcgtta
     1201 tcgaacgctt cggcctgttt gtgagcatcc tgatgcccaa gcatgaaatg cccctgcagc
     1261 gcgggctctc gttctggacg ggcctggcct tcgtcgtgct gggggcggcg gtagcggctt
     1321 attcgaccgt acagttccgc aaggtgctca agaccctgag gcccatcgag attccggagg
     1381 gttaccgggt cagtccgggc atcgtcacca acctgaccgt tgcggcgctg ggcatcgtcc
     1441 tgacggctta tttcttcatg ggtgtctaat cggcctgctt tttgcgcttc tcgcacaatt
     1501 gccgggaaaa ggtagacaaa ctcacccaat ttacggcaaa ctgcgcgcat gaaaacgatc
     1561 ctggtgttgc atggacctaa cctgaacctg ctcggaacac gcgagcccgg ggtctatggt
     1621 cgcgtcacgt tggacgaaat taaccagaat ttgaccgcat tagcggctga gaatggcgca
     1681 aatctgagct gttttcagag caatgccgaa tctgcgctgg tcgaccgcgt gcaacatgct
     1741 cgcgatgatg gcaccgattt catcgtcatc aacccggcgg ccttcacgca taccagtgtg
     1801 gcgttgcgcg atgcgctggc ggcggtcgcg atcccgttta ttgaagtaca cctttccaat
     1861 gtgtttgcac gcgaagcgtt ccgcaaggag tcgttcttct ctgatatcgc cgtcggcgtg
     1921 atcagcggac tgggcgcttc cggttacgaa ttcgcggtgc gttatgcact gaaccacaaa
     1981 gtttcttagg aggacaccat ggatttacgc aagcttaaga cattgatcga actggttgaa
     2041 ggctccggca ttgccgagct ggagatcagc gagggcgaag agcgtgtgcg catcacacgc
     2101 accgtggctg cgtcgcagca tatcgttgcc gcacctgcgc ctcaggttgt cgccgccgct
     2161 ccggtagcgg cagtggccgc cgctccggca gagcccatca aggctgccgc gccggaaggc
     2221 cacatcgtga aatcgccgat ggtgggtacc ttctaccgcg ccgcttctcc gggagccaag
     2281 gcgtttgtgg atgtcggtca gagcgtaaac aagggcgaaa cactctgcat catcgaagcg
     2341 atgaagctgc tcaacgagat cgagtccgat cagagcggtg tcatcaaggc gattctggtt
     2401 gaaaacggtc aggcggtgga gtttggccaa ccattgttcg tcatcggttg atctgcagtt
     2461 cccacggcgg cgcgattgag cgccgcatca agttcggagc caaaaagaca tgtttgaaaa
     2521 aattctgatc gcgaatcgcg gtgaaatagc gttgcgtatc cagcgtgcct gtcgagagct
     2581 gggtatcaag accgttgtgg tgcattccga ggcggatgcc gatgccaagt acgtcaagct
     2641 ggccgacgag tcggtgtgta tcggcccggc ggcatcgaac cagagctatc tgagcattcc
     2701 ggcgatcatc agtgcggcgg aagtgaccga tgcgcaggct atccatcccg gctacggttt
     2761 cctgtccgag aacgcggatt tttccgagcg ctgcgagaaa tccggtttcg tgttcatcgg
     2821 cccgcgtgcc gataccatcc gcctgatggg cgacaaggtc tccgccaaga tcgcgatgaa
     2881 aaaagcgggc gtgccctgcg tgcccggtgt tgaaggcgcg ctgccggaca atccggccga
     2941 gatcgtcaag atcgcacgca gcatcggcta tcccgtcatc atcaaggcgg ctggcggcgg
     3001 cggcgggcgg ggcatgcgcg tggtgcatac cgaagcggcg ctgctcaacg cggtgaccat
     3061 gaccaagacc gaagcgcagg ctgctttcaa caaccccatg gtgtacatgg agaagtttct
     3121 ggagaacccg cgccacatcg aattccagat tctggctgac cagcatggca atgcggtcta
     3181 tctgggcgaa cgcgactgct cgatgcagcg ccgccaccag aaggtgatcg aagaagcgcc
     3241 ggccccgcat ttgaatacgc gcttgcgcaa caagatcggc gagcgctgtg ccgaggcttg
     3301 ccgcaagatc ggctatcgcg gcgcgggcac cttcgagttc ctgtacgaga acgacgagtt
     3361 ctacttcatc gagatgaaca cgcgcg
//
LOCUS       DASUIV010000302         5683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_265669, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000302 DASUIV010000000
VERSION     DASUIV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265669"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..171
                     /locus_tag="VFW59_10825"
     CDS             25..171
                     /locus_tag="VFW59_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX5364751.1"
                     /translation="MSEKEVAKEIARLEKEMMQAAKNLEFERAAELRDQLRKLKEAVF
                     LSPL"
     gene            198..3566
                     /locus_tag="VFW59_10830"
     CDS             198..3566
                     /locus_tag="VFW59_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5364752.1"
                     /translation="MKRKSSRSRSCESLCNRYLLTFLLVAVTGFVQAQPLQLTGLPAG
                     SLGAWAEILVEEGPALSLDEAQAKQREGLFHADDRPVLTYGIGARPRWVHLALFNPTA
                     EPLPLQIVTGATWADRLDVYIVHDRQLSASWQTGDSVPRPQGLTPAIGYTLPASFAPG
                     RSDLYLRAEMVDPFVLPIELMTEQQALSNERQVHYSYGLIYGFLLALLAYNFMLFVGL
                     GERSYLYYGLYLFSLVVANLAYTGHGYAWLWPGQATLQRYVILALMVLYGCCGLLFAS
                     RFLALAEHAPRARRWVNRYILAGVALFAASVAMGSQFGAALVAFSFVTLFSIGMMLLG
                     VIAVRRGYVAGRYFLIATLFGMLGAVSTSLSVWGWVPFNTLTYHAVEYGALIEAALLA
                     LALAHRVNEMTANLSKITVSHDDLSSEVRERKRTEEMLRRSEESLKESQRIAGLGSYV
                     LDIPAGVWQSSEVLDQLLGIDDRYERSVQSWLALIHPEDRLMIEDYLGNKVIGRGEAF
                     DKEFRILRHSDQALLWLHGLGELEFDAKGHPVRMHGTIQDITEQKKIEAELRIAATAF
                     EAQEGMMITDADNRILRVNTAFTHITGYSAQEVVGQYPGMLSAGRYAADFYAAMWESI
                     NSKGSWEGEIWNRRKNGETYPEYLIITVVKDGAGTVTNYVATFNDITASKAAADEIKN
                     LAFFDPLTRLPNRRLLLDRLRHALLSSAHSARHGALLFIDMDNFKTLNDTLGHHMGDL
                     LLQEVAQRLLTCVREGDTVARLGGDEFVVVLEELSNAQMEAASQAESVIEKILDKLNQ
                     PYKISIHEHHSSPSIGVTLFSGEQFGMDELLKQADIAMYQAKKAGRNTWRFFDPSMQT
                     TVMARLTLENDLRHAILEQESIKLYYQAQVDSSGRVIGAEALARWDHPQRGFVPPDEF
                     IPLAEDCGLILPFGHWVLVTACRQLAAWATRPESAHLTLAVNVSASQFRLPTFVEEVL
                     VLIDHFGINPEKLKLEITESMLLDNINSIIAKMTELKMRGINFSMDDFGTGYSSLQYL
                     KQLPLSQLKIDQSFVRNIAVDTNDKAIVRTIIAMAQSLELDIIAEGIETQEQRELLLG
                     KGCTRYQGYLFGRPMPIEQFDASLKIFQSEQHEK"
     gene            3556..4041
                     /locus_tag="VFW59_10835"
     CDS             3556..4041
                     /locus_tag="VFW59_10835"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX5364753.1"
                     /translation="MKNNGTIAVLFVCMGNICRSPTAEAVFRQYVENAGLSEKIEIDS
                     AGTHDYHIGAPPDLRTQQAAQQRGYDMSNLRGRQVEAGDFDRFDYVLAMDQANLAILQ
                     RLAGQGEPELFLDYARHHAEREVPDPYYGGADGFERVLDMVEDAAEGLLQHIRKRHLS
                     R"
     gene            complement(4173..4676)
                     /gene="msrA"
                     /locus_tag="VFW59_10840"
     CDS             complement(4173..4676)
                     /gene="msrA"
                     /locus_tag="VFW59_10840"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5364754.1"
                     /translation="MPNQTAYLSGGCFWGMEELVRQIPGVLETEVGYTGGDVPNATYD
                     IVKTGRSGHAESLKIVFDPKRLSYRHLLFEFFRMHNPTTRNRQGNDFGTQYRSAIFYV
                     GEEQRHTAEEVIKTVESSGEWQAKIVTEVVPFRAFWRAEEYHQDYLVKHPNGYTCHYI
                     RRMNLGE"
     gene            complement(4741..5007)
                     /locus_tag="VFW59_10845"
     CDS             complement(4741..5007)
                     /locus_tag="VFW59_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEX5364755.1"
                     /translation="MVAWAIVYAKQALKDAKKLSACGLKPNAQELLAVLANNPFQNPP
                     PYEKLVGDLAGAYSRRINIQHRLVYEVFSDEHTVRVLRMWTHYE"
     gene            complement(5001..5243)
                     /locus_tag="VFW59_10850"
     CDS             complement(5001..5243)
                     /locus_tag="VFW59_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX5364756.1"
                     /translation="MHMLTASEARANLYRLIDETVDSHEPIIISGKRNNAVLLSADDW
                     SAIQETLHLLAVPGMRESIKAGMAEPLAKSAKELKW"
     gene            complement(5363..>5683)
                     /locus_tag="VFW59_10855"
     CDS             complement(5363..>5683)
                     /locus_tag="VFW59_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX5364757.1"
                     /translation="MKLGIILETNEPEQAWNGVRFANTALKQGHEVRLFLVSAGVEAE
                     SITHEKYNVRSQLHDFADNKGIILACGTCIKGRNLGESDICPISTMVDCLDMVEWADK
                     VVTF"
BASE COUNT         1179 a         1578 c         1713 g         1213 t
ORIGIN      
        1 caggctcagg ccaagtatct ggcgatgagc gagaaggaag tcgccaagga aatcgcccgg
       61 ctggagaagg agatgatgca agccgcgaag aacctggagt tcgagcgtgc cgccgaattg
      121 cgcgaccagc tgagaaaatt gaaggaggcg gtgttcctct cgccgctgtg agcacgatgc
      181 tgccgaatcg gatttttttg aaaagaaaat catcgcgctc ccggtcatgc gagtccctct
      241 gcaatcgcta tctgctgacc ttcctgttgg tcgcggtgac ggggttcgtg caggcgcagc
      301 cgctacaact caccgggctg cctgcaggat cactcggagc ctgggcggaa attctggtgg
      361 aagaggggcc agcgttgagt ctggatgagg cgcaagcgaa acagcgcgag gggctgtttc
      421 atgccgatga tcgtccggtg ctgacctacg gcatcggcgc tcgtccgcgc tgggtgcatc
      481 tggcactgtt caacccgact gccgagccct tgcctttgca gatcgtcacg ggagcaacct
      541 gggccgatcg gctcgatgtg tacatcgtgc atgaccggca gctgagcgcc agttggcaga
      601 ccggcgacag cgtcccccgc ccacaagggc tgactcctgc aatcggttac accttgcccg
      661 ccagtttcgc gccgggacgg agtgatctgt acctgcgcgc cgagatggtc gatccattcg
      721 tgcttcccat cgaattgatg accgagcagc aggcgctgtc aaacgagcgc caggtgcatt
      781 acagctatgg cctgatctac ggttttctgc tggccttgtt ggcctacaac ttcatgctgt
      841 tcgtcggtct gggcgagcgt agctatctgt actacggcct gtatctgttc agcctcgtcg
      901 tcgcgaatct ggcttatacg ggccatggct atgcctggct gtggccgggg caagcaacgt
      961 tgcagcgcta tgtgattctg gcgttgatgg tgttgtatgg ttgttgtggt ttgttattcg
     1021 ccagccgctt cctcgcgctg gccgagcatg ccccgcgcgc gcggcgatgg gtgaaccgct
     1081 atattctggc cggcgttgcc ctgttcgcag cgagtgtcgc gatggggagc cagttcggtg
     1141 ccgcgctggt cgctttctcc ttcgttacgc tgttctcgat aggcatgatg ctgctcggtg
     1201 tgattgccgt tcggcgcggt tatgtggctg gccgttattt tctgatcgcg accctcttcg
     1261 gcatgctggg ggcggtgtcc accagtcttt ccgtgtgggg ctgggtgccg ttcaatacgc
     1321 tgacttacca tgcggtggaa tacggcgcgc tcatcgaagc ggctttgctg gcgctggcgc
     1381 tggcgcaccg cgtcaacgaa atgaccgcca atctctccaa aatcacggta tcgcatgacg
     1441 acctgtcctc ggaggtccgg gaacgaaaac gcaccgagga aatgttgcgc agaagcgagg
     1501 aatccctcaa ggagtcgcag cgtatcgcgg gactgggaag ttatgtgctc gatattcctg
     1561 ccggggtgtg gcaaagctcc gaggtgctgg atcagttgct gggaatagac gatcgctatg
     1621 aacgctccgt gcaatcctgg ctggcattga tccatccgga agatcgcctg atgatagaag
     1681 actatctcgg gaataaagtc atcgggcgcg gcgaggcctt cgacaaggaa ttccgcatcc
     1741 tgcgtcacag cgatcaggcc ttgctttggc tgcatggctt gggagagctt gaattcgatg
     1801 caaagggaca tccggtgagg atgcatggca cgattcagga catcaccgag caaaagaaga
     1861 tcgaggcgga gctgcgtatc gccgccaccg ccttcgaggc tcaggagggc atgatgatca
     1921 ccgatgccga caaccggatt ctgcgggtga acaccgcgtt cactcatatc accggatact
     1981 cggctcagga agtcgttggc caatatcccg gtatgctgag cgctggtcgt tatgccgcag
     2041 atttctatgc ggcgatgtgg gagagcatca acagcaaggg gagctgggaa ggagaaatct
     2101 ggaaccggcg caagaacggc gagacctacc cggaatatct catcatcacc gtggtgaagg
     2161 atggcgcagg caccgtcacc aattacgtcg ccacgttcaa cgacatcacc gcaagcaagg
     2221 cggctgccga tgaaatcaag aatctggcgt tcttcgatcc gctcacccgg ttgcccaacc
     2281 ggaggctgtt gctggacagg ctcaggcatg ctttgctttc cagtgcccac agcgccagac
     2341 acggcgcgct gctgttcatc gacatggata acttcaagac gctcaatgac accctggggc
     2401 atcatatggg agatttgctg ctgcaggagg tcgcgcagcg cctgttgacc tgtgtgcgtg
     2461 agggcgacac ggtggcaagg ttgggcggag acgagttcgt cgtggtgctg gaagaattga
     2521 gcaacgcgca gatggaagcg gcttcgcagg cggaatccgt catcgaaaaa atcctcgaca
     2581 agctcaacca gccctacaag atatccatcc acgaacacca tagcagtccc agcatcggcg
     2641 tgacgctgtt tagcggcgaa caattcggca tggacgaatt gttgaagcag gccgacatcg
     2701 ccatgtatca ggcaaagaaa gccggacgca acacctggcg cttcttcgat cccagcatgc
     2761 agaccacggt catggctcgc ctcacgctgg aaaacgatct gcgccatgcc attctggaac
     2821 aggaatcgat caagctttat tatcaggcgc aggtcgattc atcggggcgc gtgatcggtg
     2881 ccgaggcctt ggcgcgctgg gatcatcccc agcgcggctt cgtgcctccc gacgaattta
     2941 tcccgctggc cgaggattgc ggactgatcc tgccttttgg acattgggtg ctggtcaccg
     3001 cctgccggca actggctgcc tgggcgaccc ggcccgaaag cgcccatctg acgctggccg
     3061 tgaatgtcag tgccagccag ttccgcctgc cgactttcgt tgaggaagtc ctggtattga
     3121 tcgaccactt cggcatcaac cccgaaaagc tcaagctgga gatcacggaa agcatgctgc
     3181 tggacaacat caacagcatc atcgccaaga tgaccgagct gaaaatgcgc ggcatcaact
     3241 tctcgatgga tgacttcggt accggttatt cgtcgctgca atatctcaag caactgccgc
     3301 tcagtcagct gaagatcgac cagtcgtttg tgcgaaatat cgccgtcgat actaacgaca
     3361 aggcgatcgt tcgcaccatc atcgccatgg cgcaaagtct ggaactggac atcatcgccg
     3421 aaggcatcga gacgcaagag cagcgcgagc tgcttctggg caagggttgt acgcgatacc
     3481 agggttatct gttcggcaga ccgatgccta tcgaacagtt cgatgcctca ctaaaaattt
     3541 tccaatcaga acaacatgaa aaataacgga acgatagcgg ttctttttgt ctgcatgggc
     3601 aacatctgcc gctcgccgac cgccgaggcg gtgttccggc agtatgtcga gaacgcgggg
     3661 ttgtcggaaa aaatcgaaat cgactcggcg ggcacgcacg actatcacat cggcgcgccg
     3721 cccgacctgc gcacccaaca ggcggcccag cagcgcggct acgacatgag caacctgcgc
     3781 gggcggcagg tggaagcggg cgacttcgac cgcttcgact acgtgctggc gatggatcag
     3841 gccaacctag ccatcctgca gcgtctggca gggcagggtg aaccggaact gttcctcgac
     3901 tatgcgcgcc accatgccga gcgcgaagtg cccgatccct actacggcgg ggcggacggc
     3961 ttcgagcgcg tgctggacat ggtcgaggat gcggcggaag gcttgctgca gcatatccgg
     4021 aagcggcacc tttcccgcta atcctggcta agtgggagcg gcttcagccg cgatttcccc
     4081 gtcatgtgca acgggcttat cgcggctgaa gccgctccca cgaatgtcct tggattctcg
     4141 gtcggtaccg gaggccatga ttgctgagcg cgctattcgc ccagattcat ccgcctgatg
     4201 tagtggcagg tatagccgtt cggatgtttc accagatagt cctgatggta ttcctctgca
     4261 cgccagaacg ccctgaacgg cacgacttcg gtgacgatct tcgcctgcca ttcgccggac
     4321 gattccacgg tcttgatgac ttcttcggcg gtgtgtcgct gttcttcccc gacatagaag
     4381 atcgccgaac ggtattgggt gccgaagtcg ttgccctgcc ggttgcgcgt ggtgggattg
     4441 tgcatgcgga agaactcgaa cagcagatgg cggtagctca ggcgtttcgg gtcgaacacg
     4501 atcttcagcg attcggcatg cccgctgcgt ccggtcttga cgatgtcata ggtcgcattc
     4561 ggcacatcgc cgccggtata gccgacctcg gtttccagca cgccgggaat ctggcgcacc
     4621 agctcctcca tcccccagaa acatccgccg gaaagatagg ctgtctgatt gggcatggcg
     4681 gttcctttcg cgtgggtgag ggttgagaag agcagggtga ggaggatgaa ggcgagacgc
     4741 ttattcatag tgcgtccaca tgcgtaaaac acgaacggta tgctcatcgc taaaaacttc
     4801 gtataccaag cgatgctgga tgttgatgcg gcgcgaataa gcaccggcaa gatcgccgac
     4861 cagtttttca taggggggcg gattttgaaa tgggttgttg gcaagcacgg caagcaattc
     4921 ttgagcattg ggtttcaggc cgcatgcgct cagcttcttt gcatctttta gtgcttgttt
     4981 ggcatagacg atagcccagg ctaccacttg agttccttcg cactctttgc gagcggttcg
     5041 gccatcccgg ccttgatgga ttcgcgcatg ccgggaacgg caagcaggtg caaggtctcc
     5101 tgtatcgcgc tccagtcgtc agccgagagc agcacggcat tgttgcgctt cccggaaatg
     5161 atgatgggct catgcgaatc gactgtctca tcgatgagac ggtacagatt cgcgcgcgcc
     5221 tcgctggcgg tcagcatgtg catggtgtgc tccttggaat gaagctggaa aggtacgcaa
     5281 atgcgtacgt aaagtcaagc gggatgcttg gattcggaat gcacttagtc agatggcaat
     5341 gcaacgcgcc aacaggttaa aactagaaag tgacaacctt gtcggcccat tcgaccatgt
     5401 cgaggcaatc gaccatcgtg gaaatcgggc aaatatcgga ctcgccaaga ttgcgacctt
     5461 tgatgcaggt gccgcaggcc agaatgatgc ccttgttgtc ggcgaaatca tgaagctggc
     5521 ttctcacatt atatttttcg tgagtgatcg attcggcttc aacccccgca ctcaccaaaa
     5581 acagccggac ttcatgtccc tgcttcaagg cggtgttggc aaagcgaacg ccattccacg
     5641 cttgctccgg ttcattagtt tcgaggatga tcccgagctt cat
//
LOCUS       DASUIV010000303         9154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1290125, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000303 DASUIV010000000
VERSION     DASUIV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290125"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="bcsG"
                     /locus_tag="VFW59_10860"
     CDS             <1..738
                     /gene="bcsG"
                     /locus_tag="VFW59_10860"
                     /inference="COORDINATES: protein motif:HMM:NF023088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HEX5364758.1"
                     /translation="GFEQNLAMNHDGHFDDFLQTVQAQHLTVPPMPLAGTAVTQHAFD
                     GAPIYDDFAVLMRWLDQRQKSDSARVALYYNTISLHDGNRMSGEGARLDSMETYKIRL
                     STLLDGIEKFMAGVEKSGRHAVVAMIPEHGAAIQGDKMQIPGLREIPSPKITLVPVGI
                     KVIGEGWQRQGDTVKIDAETSYLAVSQIIARMLEVSPFTRHYDPADYVDDLNVTPFVS
                     QNDSAVVMRQNGRYYWRQNDEPWSEFY"
     gene            complement(796..1887)
                     /gene="bcsZ"
                     /locus_tag="VFW59_10865"
     CDS             complement(796..1887)
                     /gene="bcsZ"
                     /locus_tag="VFW59_10865"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF008305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HEX5364759.1"
                     /translation="MRFCFAGLIWLLVPLLANAQQNCNSPWPLWEKFAGTHIQADGRV
                     VDYGAENISTSEGQSYALFFSLVADDRNRFDRILKWTSDNLSQGDLGKNLPAWKWGQD
                     SGGAWRVLDMNPASDADLWIAYSLYHAAAIWKQPRYLDTASRLLKNISEREVVDLPKL
                     GSMLLPAPYGFALNTDTWRLNPSYLPIQLLRYFSTVDRQGPWKEITLNTFRLISATSN
                     KGLVPDWVLYGTPQGFRPDPERGNDSSYDSIRVYLWWAMLNQHDPLFDALRPYVTGAT
                     YFIPGNSLPERIHVSDGTAKGKAPSGFTAALAPYRFVLYHHQTHASLNGKAGYYDYVL
                     SLFGYGWLDGRFRFNPDGSLQTASSTCSR"
     gene            complement(1919..2290)
                     /locus_tag="VFW59_10870"
     CDS             complement(1919..2290)
                     /locus_tag="VFW59_10870"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX5364760.1"
                     /translation="MQSTYKSKNGLKRLWHAFGYSMAGFSAAYKHEAAFRQEALLAAV
                     LIPLAAWLPVGHVGQALMIGSILLVIMIELLNSAIEATVDRISLESHELAKRAKDIGS
                     AAVLVSLINALVIWVLMLMTL"
     gene            complement(2338..3216)
                     /locus_tag="VFW59_10875"
     CDS             complement(2338..3216)
                     /locus_tag="VFW59_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5364761.1"
                     /translation="MTEIKSDSKPTDVDNLFERAIIDVGIPPCPLILDRFMTEIGKEE
                     PDSLRLANIIGADVGIAAGLIKTANSPFFGMRQRVRSVNEALTILGLKAASRAVAGII
                     LRNSFPNVPNLERFWDASARIAHLSGWLAQNLQIRGLRAEDAYTYGLFRDCGIPVLLG
                     RFPGYAETLNEANRDAERSFTEVEETALPTNHAMVGCILAQSWWLPEEICLAIRNHHD
                     LAILESVGSELPMLSRRLIATAQLAEHIVQKQLGLSLTQEWPKLGAACMKLLDIGEAQ
                     LETLYAEAATIATITD"
     gene            complement(3213..4508)
                     /locus_tag="VFW59_10880"
     CDS             complement(3213..4508)
                     /locus_tag="VFW59_10880"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX5364762.1"
                     /translation="MNNIDSLTGFLDRFGCLQAAVKLAADATASGEPLAVIWIDLDRF
                     KQVNESFGHLGGDEVIGNLAARFRTRISGRAELSRMGGDEFVLLVPNCDHTQAQQIAR
                     ELASTVEEPLSIGNLTLHPTASIGIAIHESGEDSLVLLERADRAMFAAKNKGGNSIVC
                     SGEEPIPGRLGITLAREELAIENNLHMALETGGFRLHYQPIIRADGSIEAVEALMRCS
                     VDGENISPAKFIPVAEKTGLIIRLGEWTLLQGAMYARLLQDADHATKVAINVSRAQLV
                     SPRFTQALHAALICSNVKPELVELELTESLFMDISDTVQKNLKNAIAAGVGLAIDDFG
                     TGYSCLANLKDIPARKLKLDRAFVTVLPEDKRALSVVRAMTQLGHELGMTIVAEGCET
                     QEQIDALLDAGVDAIQGFFYARPMPEETLIPWLQSRNMQ"
     gene            complement(4596..5816)
                     /locus_tag="VFW59_10885"
     CDS             complement(4596..5816)
                     /locus_tag="VFW59_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5364763.1"
                     /translation="MTLAMLGEAWRAMGANRLRTLLTMLGMVIGVAAVVLMMSVGQGA
                     QYAVKQSIAAMGSNLFILLSGHTTTGGARSGSGGAHTLTVGDADAIAELPGVQAVAPI
                     HPGNAQIVYGPNNWNTAVIGTTPGYLDARSWPVIAGSAFTDSDVRSATRVALIGRTAA
                     ENLFGDASPLGKTIRIRQSPFVIVGVLGIKGQGMDGRDQDDTLLIPLTTAQRQIFGSQ
                     FPGSVRMLMVQTTTQEIMPTVEKSMTGLLRQRHRIREGMESDFSLRNLTAVADSAAES
                     TRIMSLLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARERDILAQFLLE
                     AIIISVLGCFIGLLLGIGGALLTEALSGTPVIISLSSVIVAFSVAAAVGIFFGFYPAR
                     KAARLDPIEALRYQ"
     gene            complement(5813..6514)
                     /locus_tag="VFW59_10890"
     CDS             complement(5813..6514)
                     /locus_tag="VFW59_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5364764.1"
                     /translation="MSDSVIRIEALHKSYETSAGSFPVLKDVDLHISAGEFVAIMGPS
                     GSGKSTFMNILGCLDEPSAGRYLLNGRDVAELNRDELAVLRNRTIGFVFQGFNLLPRM
                     SLQDNVALPLIYAGVERDERQQRARELLTKVGLEQYFESMPNKISGGQQQRVAIARAL
                     VNRPRLILADEPTGNLDSRTGEEIMALFDNLNREGITIVLVTHEADIAQHAKRQIHFL
                     DGRIVSDHPVLERTP"
     gene            complement(6514..7686)
                     /locus_tag="VFW59_10895"
     CDS             complement(6514..7686)
                     /locus_tag="VFW59_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5364765.1"
                     /translation="MKKLLRSPVALVLLALAAGGAGLVTYRQYTAVTPEQQYRLERVE
                     TGNLSQTVSANGTINPVSLVNIGTQVSGTVKKLYVDFNSKVEKGQILLELDDALLAAQ
                     QKQSQANVQSATAALELADANEARMRNLYAQEYVSRQELDTAVQAKKSAEAQLQLTRA
                     AVEKDRANLSYSVIRSPVSGVVVDRTVDVGQTVAASLQTPTLFKIAQDLAKMQIDANF
                     AEADIGSIRVGQTARFSVDAFANRSFQGAVRQIRLNPTTQQNVVTYDVVIDVNNPEQL
                     LLPGMTAYVSIAVAERKDALLVPNGALRFRPADGSKRSDANENGAKAKSNGFSGKVYV
                     LRQGKLAPVNVTLGITDNRNTEVIGGELKAGDQVVVGDAQTANNAASSAAPRMRMF"
     gene            complement(7683..>9154)
                     /locus_tag="VFW59_10900"
     CDS             complement(7683..>9154)
                     /locus_tag="VFW59_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5364766.1"
                     /translation="NAPNKFASAGQAVTIDQWNRKALRMRLRLKHSVLFALLCLWSGA
                     AAHAEGYDPLGTEAELPLKPAVRIGGALGGPCADAKPDHALDLMEIVNLALCSNPQTR
                     EIWASARVQAAQLGVSRGSYLPSVSLSVSDNRNGAAQRSYGLTLSYLLYDFGTRAANL
                     ENARQLFAAASATRDNVVQSIFLAAVRAFYQAQATRAALDAAQESERAAKQSFSAAEA
                     RYQAGSATPADKLQAQTAWSQATLNRITAEGNLGKANGGLANIIGLEPNHRVVLDTEN
                     SGSIPVNFERDVNSLIAEAKQRRPDLIAAEAQVKAAEASADAARAAGKPSLSLTTTTT
                     LVQSGGTNSRGSSVGVNLSVPLFSGYGPTYRIRAAEAQVEAQQAQKERLSLQVALDVW
                     NAYQDLITATQSLRTTADLLNSAEQSWRVALGRYQAGVGSMLDVLTAQSALASARGQR
                     IQSTLNWNVSRATLAQAMGTLDESLLQTLSPAVSHQEQP"
BASE COUNT         1694 a         2641 c         2877 g         1942 t
ORIGIN      
        1 ggtttcgagc aaaatctggc gatgaatcat gatggtcatt tcgacgattt cctccagacc
       61 gtgcaggcgc agcacttgac cgttcccccc atgccgctgg ctggcacagc ggttacacag
      121 cacgcctttg atggcgctcc catctacgac gatttcgcgg tattgatgcg ctggctggat
      181 cagcgccaga agtcggacag tgcgcgtgtt gcgctgtatt acaacacgat tagcctgcac
      241 gatggcaatc ggatgtcggg ggagggtgca aggctggata gcatggaaac ctacaagatt
      301 cgtctgtcca cactgctgga tggcatcgag aaatttatgg cgggggtgga aaaatccggc
      361 aggcatgccg tcgtggcgat gattcccgag cacggtgcag ccattcaggg cgacaagatg
      421 caaatccccg gcctgcgtga gattccgagt ccaaagatta cgctggttcc cgtcggtatc
      481 aaggtcatag gcgaggggtg gcagcgtcag ggcgacaccg tgaagatcga cgcggaaacc
      541 agttatctgg cggtcagcca aatcatcgcg cgcatgctgg aagtctcacc cttcacccgc
      601 cactacgatc ctgccgacta tgtcgatgac ttgaacgtga cgccgttcgt gtcgcagaac
      661 gatagtgccg tcgtcatgcg ccagaatggc cgttactact ggcggcaaaa cgatgaaccg
      721 tggagcgagt tttattgatc ctgcttctcg gatcgtggtt aacaaagcat gatctgcagg
      781 agggggttaa gcgttttatc tggaacaggt gctcgatgct gtctgcaggc tgccgtccgg
      841 attgaaccgg aagcgtccat ccagccagcc ataaccgaac aggctcaaca catagtcgta
      901 atagcccgct ttgccattga gtgaggcgtg tgtctgatgg tgatacaaaa cgaatcggta
      961 gggtgctaac gccgctgtaa agccgctggg agcctttccc ttcgcggtgc catccgatac
     1021 atggatgcgt tcgggcaatg agtttcccgg aataaagtag gttgcacccg tcacataggg
     1081 acggagcgcg tcgaacaatg ggtcgtgttg attgagcatg gcccaccaga ggtaaaccct
     1141 gatggaatca tagctgctgt cattcccccg ttcgggatca ggccggaatc cttgcggtgt
     1201 tccgtataac acccagtcag gaacaagccc cttgttcgag gtggcggaga tcagccggaa
     1261 cgtgtttagc gttatttcct tccatgggcc ttgcctgtcg acagtggaaa aatagcgcaa
     1321 caactggatc ggcaaatagc tggggttcag tcgccaagta tctgtgttca aggcgaagcc
     1381 gtatggggcg ggcagcagca tgctgcccag ttttggcaag tccaccacct cgcgctccga
     1441 tatgtttttc aacaagcgcg atgccgtatc gaggtagcga ggttgtttcc agatagctgc
     1501 cgcatggtag agggaatagg caatccacag gtcggcatcg ctggcgggat tcatgtccag
     1561 aacgcgccat gcaccgccgc tgtcctgccc ccatttccag gccgggagat tcttgccgag
     1621 atccccctgc gacagattgt cgctggtcca cttcagaatg cggtcgaagc gatttctgtc
     1681 atctgcgacc agcgagaaaa acaacgcata agactggcct tcagaagttg agatgttctc
     1741 cgcgccgtaa tccaccacac ggccatcggc ttgaatgtgc gttccggcaa atttctccca
     1801 caacggccat gggctgttgc agttctgttg tgcattggcc agcaagggaa caagcaacca
     1861 gatgagcccg gcgaagcaga atctcaagac attctctcgg tgaattgagg cgctgatgtt
     1921 acagcgtcat gagcatcagc acccagatca ccaaggcatt gatcaggctg accagtacgg
     1981 ctgcgctgcc gatgtccttg gctcgcttgg cgagttcgtg actctccaga gagatgcgat
     2041 cgaccgtggc ctcgatggcg gaattcagca gctcgatcat gatgacgagc aggatgctgc
     2101 ctatcatcaa tgcttggccg acatgaccga ccggcaacca tgccgccagc ggaatcagga
     2161 cggccgccag caacgcctcc tgacggaatg ccgcctcatg tttgtatgcg gcactgaagc
     2221 cggccattga gtagccgaag gcatgccaca atcgtttcag gccatttttg cttttatagg
     2281 tactttgcat gggtgaagat gtcggccggg ttctttgcga aggagagggg ccgagggcta
     2341 atcggtgatg gtcgcaatgg tggccgcctc ggcatacaaa gtttcgagtt gcgcttcacc
     2401 gatatccagc agcttcatgc aggcggctcc cagcttgggc cattcctgcg tcaggcttaa
     2461 gcccagttgc ttctgcacga tatgctcggc aagctgggct gtcgcgatga gtctgcggct
     2521 cagcatgggc agttcggagc cgaccgattc caggatggca aggtcgtggt gattgcggat
     2581 ggccagacag atctcttctg gcagccacca gctttgtgcc aggatgcaac ccaccatggc
     2641 gtgattggtc ggcagggcgg tttcttccac ctcggtgaag ctgcgctcgg cgtcgcggtt
     2701 ggcttcgttc agtgtttctg cgtagccggg gaagcgaccc agcagaaccg ggatgccgca
     2761 gtcgcggaac aggccatagg tgtaggcatc ctcggcgcgc aggccgcgta tctggagatt
     2821 ctgggccagc cagccggaca ggtgcgcgat acgcgccgaa gcgtcccaga aacgttccag
     2881 attgggcacg ttgggaaagg aattgcgcag gatgatgccg gccacggcac ggctggctgc
     2941 cttcagtccg agtatcgtga gcgcttcatt caccgagcgt actcgttggc gcatgccgaa
     3001 aaacggggag ttcgcggtct tgatcaggcc cgctgcgatg ccgacgtcgg caccgatgat
     3061 gttcgccaga cggaggctat cgggttcctc tttgcctatc tcggtcatga accggtccag
     3121 aatcagcggg cagggaggaa tgccgacatc gatgatggcg cgttcgaaca ggttgtcgac
     3181 atctgtcggt tttgaatcgg atttaatttc ggtcattgca tgttccttga ttgcagccag
     3241 gggatcagcg tctcctccgg catcggtctg gcgtaaaaga agccctggat ggcatcgacg
     3301 cccgcatcga gcagcgcatc gatctgttct tgtgtttcgc agccttcggc gacgatggtc
     3361 atgcccagct cgtgacccag ctgggtcatg gccctgacca cggacagggc gcgtttatct
     3421 tctggcagca cggtgacgaa ggcgcggtcc agtttgagtt ttctcgccgg aatatccttc
     3481 aggttcgcca gacaggaata gcccgtgccg aagtcgtcga tggccagtcc aacgcctgcg
     3541 gcgatggcgt ttttcagatt cttctggacc gtatcggaaa tatccatgaa cagcgattcg
     3601 gtcagttcca gttccaccag ttcgggtttg acgttggagc agatcaacgc cgcatgcagc
     3661 gcctgggtga atcgcggcga gaccagttgt gcgcgggaga cattgatggc gaccttggtg
     3721 gcgtgatctg cgtcctgcag caagcgtgcg tacatcgcgc cctgcaacag ggtccattcg
     3781 cccagacgga tgataagccc ggttttctcc gcaacgggaa tgaacttggc gggcgagatg
     3841 ttttcgccgt cgacagagca gcgcatcagt gcctcgacgg cttcgatact gccatctgcg
     3901 cgaatgatgg gttgatagtg caggcggaac ccgccggtct ccagcgccat atgcagatta
     3961 ttttcgatcg caagctcttc gcgtgcgagg gtgatgccga ggcgtcccgg gatcggctct
     4021 tcgcccgaac agacgatgct gttgccgcct ttgttcttgg ccgcaaacat ggcgcggtct
     4081 gcgcgttcca gcaggaccag agaatcttcg ccggattcgt ggatcgcgat gccgatactc
     4141 gcggtgggat gcagggtcag gttgccgatg ctgagcggtt cctcgactgt gctggcgagc
     4201 tcgcgggcaa tctgttgtgc ctgggtgtgg tcgcaattcg gaacgagcag cacgaactcg
     4261 tcgccgccca ttctggagag ctcggcgcgc ccgctgatcc gggtgcgaaa gcgtgcggcg
     4321 agatttccga tcacttcgtc accgcccaga tgaccgaacg actcgttgac ctgcttgaat
     4381 cgatccagat cgatccagat cacggcgagc ggttcgccgc tggcagttgc atcggccgca
     4441 agttttactg cggcctgcaa acaaccgaac ctgtccagaa aaccggtgag gctatctatg
     4501 ttattcatat cacgatgcga agcacgcaag atgtgcgggt gtgcagaata aaccaaatcg
     4561 gaagtttgtc catagcaggg gcagggtgat ttctgctatt ggtagcgcag cgcctcgatc
     4621 ggatccagtc tggccgcttt gcgtgccggg tagaagccga agaagatgcc cactgctgcc
     4681 gcaacgctga atgcaacgat caccgagctc aacgagatga tgaccggtgt gccgctcaac
     4741 gcctcggtga gcagggcacc gccgatcccc agcaacaacc cgatgaagca gccgagcacc
     4801 gagatgatga tcgcctccag cagaaattgc gcgaggatgt cgcgctccct cgcgccgatc
     4861 gccatgcgga taccgatctc gcgggtgcgt tcggtgaccg agaccagcat gatattcatg
     4921 atgccgatgc cgcctaccag cagcgacacc gaggcgatcg cgccgagcag cagcgacatg
     4981 atgcgcgtgc tctcggcggc ggaatcggcc acggcagtca ggttgcgcag cgagaagtcg
     5041 ctctccatgc cttcgcggat acggtggcgt tggcgcaaca gtccggtcat ggatttttcc
     5101 acggtaggca tgatctcctg tgtggtggtc tgcaccatga gcatgcgtac cgagcctggg
     5161 aactggctgc cgaatatctg gcgttgcgcg gtggtgagcg ggatcagcag cgtgtcgtcc
     5221 tgatcccgcc cgtccatgcc ctgtcccttg atgcccagca cgccgacgat cacgaacggg
     5281 ctctggcgga tgcggatggt cttgcccagc ggactcgcgt caccgaacag attttctgcc
     5341 gccgttctgc cgatcaatgc aacgcgcgtg gcggagcgca catccgaatc ggtgaacgcc
     5401 gatccggcga tcaccggcca cgagcgcgca tcgagataac cgggagtggt gccgatcact
     5461 gcggtgttcc agttgttcgg gccatagacg atttgtgcgt tgccgggatg gatgggggcg
     5521 acggcttgca cgccgggtag ttcggcgatg gcatctgcat cgccgaccgt cagcgtgtga
     5581 gcgccgccgc tgccggagcg cgcgccgccg gtggtcgtgt gtccggataa caggatgaac
     5641 agattgcttc ccatcgcggc gatgctctgt ttgacggcgt attgcgcgcc ttgtcccacc
     5701 gacatcatca gcaccaccgc cgcgacgccg atcaccatgc ctagcatggt cagcagcgtg
     5761 cgcagccggt tggctcccat cgcgcgccag gcttcaccga gcatcgcgag cgtcacgggg
     5821 tgcgctccag aaccggatgg tcgctgacga tgcgtccgtc gagaaaatgt atctgacgct
     5881 tggcgtgttg tgcgatgtcg gcctcgtggg tcaccagcac gatggtgatg ccttctctat
     5941 tgaggttatc gaacagcgcc atgatctctt cgccggtgcg gctgtcgagg ttgccggtag
     6001 gttcatcggc gaggatcagg cgcgggcggt tgaccagtgc ccgcgcgatg gcgacgcgct
     6061 gctgttgtcc accggagatc ttgttgggca tggactcgaa atactgctcc agcccgacct
     6121 tggttaacag ttcgcgtgcg cgctgctggc gttcgtcgcg ttcgactccg gcgtagatca
     6181 gcggcagcgc gacgttgtcc tgcagcgaca tgcgcggcag caaattgaag ccctgaaaca
     6241 cgaatccgat ggtgcggttg cgcaggacgg cgagttcgtc tcggttcagc tcggcgacat
     6301 cgcggccgtt caacaggtag cgtccggcgg agggttcgtc cagacagccc aggatgttca
     6361 tgaaggttga tttgccggat ccggacggtc ccatgatggc gacgaattca cccgccgaaa
     6421 tatgcagatc gacatccttg agcaccggga acgacccggc ggaagtctcg taggacttgt
     6481 gcagcgcctc gatgcgaatg acgctgtcgc tcatcagaac attctcatgc gcggggcggc
     6541 gctgctcgcc gcgttgttcg ccgtctgcgc atcgccgacc accacctggt cccccgcctt
     6601 gagttcgccg ccgataacct cggtgttgcg gttgtcggtg atgccgagcg tcacattgac
     6661 cggcgccagt tttccctgtc tgagcacata caccttgccg gaaaaaccgt tggatttcgc
     6721 cttcgcgccg ttttcattcg catcgctgcg cttgctgcca tcggcaggcc tgaaccggag
     6781 tgctccgttg ggaaccagca gcgcatcctt gcgttccgcc acggcgatgc tcacataggc
     6841 ggtcatgccg ggcagcagga gctgctcggg gttgttcacg tcgatcacca catcgtaggt
     6901 cacgacgttc tgctgggtgg tcggattcag tcgtatctgg cgcaccgcac cctgaaagct
     6961 gcggttggcg aaggcgtcca cgctgaagcg cgccgtctga ccgacgcgta tgctgccgat
     7021 gtccgcctcg gcgaaattcg cgtcgatctg catcttcgcc agatcctgag cgatcttgaa
     7081 cagcgtgggg gtttgcaggc tggcggcgac ggtctgaccg acatccacgg tgcgatccac
     7141 caccacaccg gatacggggg agcggatcac cgaatagctc aggttggccc ggtccttttc
     7201 gacggcggcg cgtgtcagtt gcaactgcgc ttcggcggat tttttcgcct gcacggcggt
     7261 atcgagttcc tggcgcgaca cgtattcctg cgcgtacaga ttgcgcatgc gcgcctcgtt
     7321 ggcatcggcc agctccagcg ctgcggtggc gctctgtaca ttggcctgac tctgtttctg
     7381 ctgtgcggcg agcagtgcgt cgtccagttc cagcagtatc tggccctttt cgaccttgct
     7441 gttgaaatcg acgtagagtt tcttcaccgt gccggatacc tgggtgccga tgttcaccag
     7501 cgataccgga ttgatcgtgc cgttggccga gaccgtctgg ctgaggttgc cggtttcgac
     7561 gcgctccaga cggtattgct gttccggcgt taccgccgtg tattgtcgat aggtgaccag
     7621 tccggcaccg cctgccgcga gggcgagcag cacaagggcg acgggagagc gcagcagttt
     7681 tttcatggtt gttcctgatg agagacggca ggcgacagcg tttgcagcag gctctcgtcg
     7741 agagtgccca tcgcctgcgc cagcgtggcc cggctgacat tccagttcag cgtggactgg
     7801 atgcgttgcc cgcgtgcgga agccagtgcg ctctgggccg tcagcacgtc cagcatgctg
     7861 ccgacgccgg cctgatagcg gcccagcgcg acgcgccatg actgctcggc gctgttgagc
     7921 agatcggccg tggtgcgcag cgactgcgtg gcggtaatga gatcctgata ggcattccac
     7981 acgtccagtg cgacttgcag agacaggcgt tccttttgtg cctgctgcgc ttccacctga
     8041 gcctcggccg cacgtatgcg ataggtcggg ccatagccgg agaaaagcgg cacgctcagg
     8101 ttgacgccca cagaggaacc gcgtgagttg gtccctccgc tctggaccag tgtggtcgtc
     8161 gtggtcagcg agaggctcgg ttttcccgcc gcgcgtgccg catccgcact cgcttctgcc
     8221 gccttcacct gagcctcggc ggcgatcaga tccgggcgcc gctgtttggc ttccgcaatc
     8281 agcgagttca cgtcgcgttc gaaattaacg gggatgctcc cggagttttc cgtgtccagc
     8341 acgaccctgt gattgggttc cagcccgatg atgtttgcca gtccgccatt ggccttcccg
     8401 agattgcctt ccgcagtgat gcggttcagc gtggcctggg accatgcggt ctgtgcttgc
     8461 agcttgtcgg cgggcgtggc actgcctgcc tgatagcgcg cctcggcggc ggagaagctt
     8521 tgctttgcgg cgcgttcgga ttcctgtgcc gcatccagtg ctgcacgagt tgcctgcgcc
     8581 tgatagaagg cccgtaccgc agcgaggaat atggactgca cgacattgtc gcgcgtggca
     8641 ctcgctgcgg cgaatagttg gcgtgcgttt tccagattgg cggcgcgggt gccgaagtcg
     8701 tacagcaggt aggacagtgt cagaccgtaa ctgcgctggg cggcgccgtt gcggttgtcg
     8761 gagacgctca ggctgacgct gggcagatag ctaccgcggc ttaccccgag ctgcgcggcc
     8821 tgtacccgcg cgctggccca tatctcgcgc gtctgcgggt tgctgcatag cgcgagattg
     8881 acgatctcca tgaggtcaag tgcgtgatcg ggtttggcat cggcacaggg gccacccagg
     8941 gcgccgccga tgcgtaccgc aggtttgagc ggtaattcgg cctcggtacc gagcgggtca
     9001 taaccttcag cgtgagcagc ggcaccgctc cagagacaga gcagggcgaa taaaacggaa
     9061 tgtttcaggc gcagtcgcat gcgaagtgct ttcctattcc attgatcgat cgtgaccgcc
     9121 tgtccggcgc tcgcgaattt gttcggcgca ttat
//
LOCUS       DASUIV010000304        12966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_915090, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000304 DASUIV010000000
VERSION     DASUIV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 12966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12966
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915090"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..877
                     /locus_tag="VFW59_10905"
     CDS             83..877
                     /locus_tag="VFW59_10905"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX5364767.1"
                     /translation="MDDLLTIAGKSYSSRLLVGTGKYKDFTETRAALDASGAEIVTVA
                     IRRTNLGQNANEPNLLDAVPPSKFTYLPNTAGCYSADDAVRTLRLARELLDGHSLVKL
                     EVLGDPQSLYPNVIETIAAAKTLVAEGFQVMVYTSDDPIVAKQLEDIGCVAIMPLASL
                     IGSGMGILNPWNLQLIMDRVQVPVIVDAGVGTASDAAIAMELGCAGVLMNTAIAGAQN
                     PVLMASAMKKATEAGREAYLAGRMPKKLYSASPSSPTAGIISSVRS"
     gene            945..1643
                     /gene="trmB"
                     /locus_tag="VFW59_10910"
     CDS             945..1643
                     /gene="trmB"
                     /locus_tag="VFW59_10910"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX5364768.1"
                     /translation="MTDTTDLKNRPIRSFVLRQGRVSPGQQRAVETLMPQYGISYAPQ
                     LLNLDQAFGRSAPKILEIGFGMGNSTATIAQAAPGNDYLALEVHTPGVGNLLKLIDAQ
                     QISNIRIIQHDAVEVLRDMIGDGALDGVHIFFPDPWHKARHNKRRLIQSPFIARLVQK
                     IKPGGYIHVATDWQDYAEQVLAVLSAEPLLENTSTDYAPRPDYRPLTKFEKRGINLGH
                     GVWDLVFRRISTPA"
     gene            1734..2153
                     /locus_tag="VFW59_10915"
     CDS             1734..2153
                     /locus_tag="VFW59_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HEX5364769.1"
                     /translation="MKKLSYVLLALLALASISAQADVVTATKAAGDYGLWAKKLNPKS
                     TLSAEAGRAFYTKKVEVAGKDLSCAACHTDNPANPGKHNETGKPIKPMAPAVNPQRFS
                     NIQKSAAGFSKHCQDLYGKDCSPQDKGDFITYLLTIK"
     gene            complement(2212..2634)
                     /locus_tag="VFW59_10920"
     CDS             complement(2212..2634)
                     /locus_tag="VFW59_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364770.1"
                     /translation="MQSSSREKKNAARGRSAWGSVRGNAIVHLVWCAVVLSGCGGEVG
                     PDKTEREKAKSFDQAIADARCLSCHLPGNKVGAPSWKEVAKKYKKDKKDVAESYLANK
                     IAHGGSGVWGRVNMPPHAELSQEELAVMARGILAAGKK"
     gene            complement(2621..4255)
                     /locus_tag="VFW59_10925"
     CDS             complement(2621..4255)
                     /locus_tag="VFW59_10925"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5364771.1"
                     /translation="MIYCLLIQSSRNRQGVPVRKENIVAGEAIRIGRSMACKLRLLDQ
                     QVALHHATLRQSKEGVIYIETEGDAALSVEGVAAQRAELRPGVRVEIGPYLLIVDPAS
                     AGSNIALVVEMIRPPAEQTPVRREPLTLEALGMSKRRLGFALAAGILFLFLLLPLLPG
                     LSSSLDKAQAALPVTLTDSWNPGPMSSGHRAFGEQCSACHQRGFRAVADEACTSCHKK
                     VEKHLTDDALHAALFKDMRCTACHVDHKGKAGLMPYDSARCVACHGDIKSKNARATVA
                     NVHRFTRDHPPFRLAVQNGKDGRPLIRVLQERKVSESPGLKFSHQVHLDKAGVSSPEG
                     DTVMSCHDCHRPDEAGIHFEPMSMKRSCQQSGCHSLDFAAPVAGEVPHGSVREAMSKM
                     RSYYVQWLAESPENMAECGQNGDPGNSLQGTFACASDLAQKNAAVSLFGKDGSCSECH
                     EIEGSDDSAVPWKVVPVRINRNWHAKSVFPHAKHDTTECASCHDKINSKTSADISMPT
                     IEKCRECHVDDKAVKGKVSSTCYSCHRFHGGGDAVK"
     gene            complement(4262..5227)
                     /locus_tag="VFW59_10930"
     CDS             complement(4262..5227)
                     /locus_tag="VFW59_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364772.1"
                     /translation="MKMAYRSGLLSFMNFRYLKLSGLLMAASIGAYVFAPLGSEPYGG
                     TWLGYLLGIVSALIVLLLLWYGIAKRRRPRVHHREMSPAKERRRQGSVRLNSFLRRWE
                     DRGRPRPGDPWSRGGTLQGWLSLHINLGGSLIVLATLHTGFQFGWNVHTLSYVLMLLV
                     IASGFYGLYAYLKYPRLITLNQGEETLEDLLLQIDELDELARVQALALPDEVNALVQK
                     SRQGTRLGGSLLQQLSGRQRGCPTTAAARQMLKLGPKYIKHEQPKLLRELYGVLLRKE
                     SLVLRARREIMLKARMQFWLYLHVPLTVALLAALVAHVVAILYYW"
     gene            complement(5224..7641)
                     /locus_tag="VFW59_10935"
     CDS             complement(5224..7641)
                     /locus_tag="VFW59_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5364773.1"
                     /translation="MADHYKIVIIGAGPAGMSAAARAAELGVSHVLLEAAPHPANTMH
                     HYQRRKLVMAEPAVLPLRSTLTFGAGIREHILQCWQEELVAHRVNLRVNAAVSGISGR
                     LGAFQVTLATGEVLSAEFVVLAIGLQGNIRKLGVPGEDLPGVQYQLDDPEAFNGETIV
                     VIGAGDAGVENALALAEHNRVIFINRQEEFTSCSKTNFDLLMAEVTKGRIETQVSTWA
                     ERVEQSTGRDFPLVLVVHTSQGLARIECHRVIARLGAKPPRQLVESFGVQFPNGDSTA
                     VPQLTEQYESNVPGLYVVGALAGYPLIKQAMNQGYEVVEYIQGNPVEPADEALLRDKF
                     SGVPEVRSVNDGIALIRRNQPLFATLTTLQLREFMLDSTLWVPQCGEVLFKRNDYSSS
                     FFSILSGSVTIHTEGKDGAVSTFRLGAGEFFGEMGLLSGRRRSGTAVAGEGCVLVETP
                     RRSMLKLLYAVPGVRRRLDEVALKRIVQNSLDAALPEHEFEHLVQDAQFKQYAVGEAL
                     FKEGDQVDGLYLIRRGSVTVSRLIAGKEVMLAYVSAGNYVGEMALVSNTLRLATVRAA
                     SPTDVVLLEAGRVRAVMERNASIRGKVDSRYLERIGADVLPEDTSFSGLIKFLMTQGV
                     GEATDVLLIDYERCIRCDNCETACADVHGGISRLDREAGMTYEQIHLPTSCRHCEHPH
                     CMKDCPPDAIQRAVNGEVFISDSCIGCGNCQKNCPYGVIQMAVQSEPQQGGIWQILFG
                     SGKKAVVEADASVSKKAVKCDMCKGIRGGPLCVNACPTGAAFRVSPEGFFSHVGWASD
                     ADREDRS"
     gene            complement(7668..8663)
                     /locus_tag="VFW59_10940"
     CDS             complement(7668..8663)
                     /locus_tag="VFW59_10940"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5364774.1"
                     /translation="MKITVIGAGAWGTALAISLAANHRVTLWARNAGQVEAMRGARSN
                     QRYLPGIPLPADLELSADLPAALADAELAIVAVPTSAFRTTLQLLARSSGNFGVVWVC
                     KGFEAETSLLPHQVVAEVLPARFSYGVLSGPSFALEVARGLPTALTLASTDVEFARST
                     ALALHHAHLRIYASSDLVGVEVGGAVKNVMAIAAGICDGMGLGHNARAALLTRGLAEI
                     ARLGLKLGGTSETFSGLSGVGDLILTCTGDLSRNRQVGLLLAQQHDLPEILRRLGHVA
                     EGVYTVREVHQLAQRLDVAMPICEAVYRILYEHIPVSDVITALLSRAPTQEFDGD"
     gene            complement(8724..9164)
                     /locus_tag="VFW59_10945"
     CDS             complement(8724..9164)
                     /locus_tag="VFW59_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5364775.1"
                     /translation="MKLPRLLALFLLLCGGAAYAVDFVSVTEDNAILYDAPSLKAKKL
                     FVVNRYMPFEQVVTLQDWVKVRDRSGGLYWIEARALGSKRYLFALPPLLDVHAAADFS
                     APVLFKVRQQVALERLESTGTGWIKVRHLDGETGYVRSSEVWGN"
     gene            complement(9164..9628)
                     /gene="secB"
                     /locus_tag="VFW59_10950"
     CDS             complement(9164..9628)
                     /gene="secB"
                     /locus_tag="VFW59_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX5364776.1"
                     /translation="MTEAAQQNTQPLFNMEKLYVKDLSLEIPNAPLIFLERENPLIDV
                     QLHTQAASIEEGMFEVSVTSTVTAKIGEKVMFLIEAKQAGIFQIRNIPAAELEPILAV
                     MCPNILFPYLREVVSDASVRGGFAPVLLNPINFDVLYQQQKQQQSGATETTH"
     gene            complement(9648..9905)
                     /gene="grxC"
                     /locus_tag="VFW59_10955"
     CDS             complement(9648..9905)
                     /gene="grxC"
                     /locus_tag="VFW59_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX5364777.1"
                     /translation="MPKVVMYCTEICPYCVRAESLLQRKGVTGIEKIRIDLHPEQRAV
                     MMEKTGRRTVPQIYIGDRHVGGYDDLAELDRAGELAPLLAE"
     gene            complement(9905..10315)
                     /locus_tag="VFW59_10960"
     CDS             complement(9905..10315)
                     /locus_tag="VFW59_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5364778.1"
                     /translation="MQFITSNIFPISIALFSGAMLFWSLFGNRIRGIQEVDCTAALQL
                     VNHKSALVLDVREEGEYKSGHILNAKLIPLGKLAERLGELEKYKEQPIVVVCRSGNRS
                     ASACARLAKNGFASPYNLAGGMMAWQKASLPVKK"
     gene            complement(10299..12056)
                     /locus_tag="VFW59_10965"
     CDS             complement(10299..12056)
                     /locus_tag="VFW59_10965"
                     /inference="COORDINATES: protein motif:HMM:NF023274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HEX5364779.1"
                     /translation="MQITLADISLALVGVMWVFPFLHYRHQYPLTTFYQEWWCALLGV
                     AALTLLVGRAYWQRPEIPRIAQLPIALAAVALLQLGLGMVAYFDQALLYVLYLLFAAL
                     LMLLGAALRDRFGMARLAQVLAVFLLIGAELSAAIGVLQHFRWHTPFDSVVVVKVSAD
                     VFGNLAQRNHFANYIALGLISLGLLHQLGLKGRYALLLALPLLLTMTLSGSRSSWLYL
                     SMMATLAWWWGRRDQTMRPLLRYSLLLIAGFGAMHLLVQLPFMAGAGGSTDTVQRLFG
                     EDAGGGIRLYLWRESWLIFSQSPWLGVGFGQFAWHHFQLLPVLQQGNIQGLYNNAHNL
                     VFQLAAETGIAGLLILFTALAIWLHGARRASLDAAHWWGYAALGVLAIHSLLEYPLWY
                     AYFLGIASILLGALDETRYRLELRAVGRLSVAAVLLLGLLTLLQFIGSYHQLGRVLGL
                     RPASAADHATVERIRDGLVAVHGNSLLSPYAELFMSSMIDVTEERKQDKLALNTRVMR
                     FVPIATAAYRQSLLLALNDRPQQAMLAMEQAIWSYPGEFASVRQQLASLAEKSPERFA
                     ALLEFALEKEQEQARAVHH"
     gene            complement(12074..12286)
                     /locus_tag="VFW59_10970"
     CDS             complement(12074..12286)
                     /locus_tag="VFW59_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX5364780.1"
                     /translation="MNAERIVRIVAGFFVMLSLLLGVEASPLFVSQKALWFTGFVGFN
                     LFQSGFTQICPLTNILSLFGIKSRSC"
     gene            complement(12318..>12966)
                     /locus_tag="VFW59_10975"
     CDS             complement(12318..>12966)
                     /locus_tag="VFW59_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5364781.1"
                     /translation="LDSPLYGMFDINGKTSGAALEQGGVLESYFFKQPSDPYAGYALK
                     WDGEWQVTFETFRDMGIAYGVGLILIYLLVVAQFKSYLVPLIIMAPIPLTIIGVMPGH
                     ALFGSQFTATSMIGMIALAGIIVRNSILLVDFVNLQLRDGVDLQHAVINAAGARAKPI
                     ILTGLAAMLGALFILDDPIFNGLALSLIFGILVSTMLTLVVIPVLYYATLYKKYP"
BASE COUNT         2788 a         4241 c         3578 g         2359 t
ORIGIN      
        1 gtcacgcccc cgcactgtat ttctgctccg cgcactttct ccgcttccta cgttcaactc
       61 atttttcagg ttggctttcc ccatggacga tttgctgacg attgctggca agagctattc
      121 ctcccggctg ctggtgggaa ccggcaagta caaggacttc accgagacac gcgcggcgct
      181 ggacgccagc ggcgccgaga tcgtcacggt ggcgatccgc cgcaccaatc tcggccagaa
      241 cgcgaacgag cccaacctgc tcgatgccgt gccgccgtcg aaattcacct atttacccaa
      301 caccgcaggc tgctatagcg ccgacgatgc ggtgcgcacg ctgcgcctgg cgcgcgagtt
      361 gctggacggc cactcgctgg tcaagctgga agtgctgggc gatccgcagt ctctgtaccc
      421 caacgtgatc gagaccatcg ctgcggcgaa gacgctggtg gccgaaggct ttcaggtgat
      481 ggtctacacc tcggacgacc ccatcgtcgc caaacagctg gaagacatcg gctgcgtggc
      541 gatcatgccg ctggcctcgc tgatcggttc gggcatgggc atcctgaacc cctggaatct
      601 gcagctgatc atggaccgcg tgcaagtgcc ggtgatcgtc gatgcgggcg tgggcacggc
      661 ttccgacgcg gcgatcgcaa tggagctggg ctgcgcgggc gtgctgatga ataccgcgat
      721 cgcgggcgca cagaatccgg tgctgatggc ctcggcgatg aagaaggcta cggaagcagg
      781 acgcgaagcg tatctggccg ggcgcatgcc gaagaagctg tacagcgcca gcccgagctc
      841 gccgaccgca ggcatcatca gctcggtacg ttcgtagaac acggcacgca atatcgtagg
      901 ggcacggcgc gccgtgcccc tacccccgaa aatacccaga tatcatgacc gacaccaccg
      961 atctcaaaaa cagacccata cgcagcttcg tgctgaggca gggccgcgtc tcgcccggac
     1021 agcagcgcgc cgtcgagact ctgatgccgc aatacggcat ttcctacgcg ccacaactgc
     1081 tgaacctcga ccaggctttc ggccgcagcg cgccaaagat tctggagatc ggtttcggca
     1141 tgggcaattc caccgcaacc atcgcgcagg cagcccccgg gaacgactat ctggcgctgg
     1201 aagtgcatac acccggcgtg ggcaatctgc tcaagctgat cgatgcgcaa cagatatcca
     1261 acatccgcat cattcagcac gatgcggtgg aagtgctgcg cgacatgata ggagacggtg
     1321 cgctggacgg cgtgcatatc ttctttcccg acccctggca caaggcgcgc cacaacaagc
     1381 gccgcctgat ccagtcgccg ttcatcgcca ggctggtgca gaagatcaaa cccggcggct
     1441 acatccatgt cgccaccgac tggcaggatt atgccgaaca ggtgctggcg gtattgagcg
     1501 ccgagcccct gctggaaaac acctcgaccg actacgcacc gcgcccggac taccgcccgc
     1561 tcaccaagtt cgagaaacgc ggcatcaatc tgggacacgg ggtatgggat ctggttttca
     1621 ggcggatttc gacgccggcc taaagcctcc gccgagttag attgacaggc caaggcttgt
     1681 cgaaagacaa tgccagcgca ccaccaaccc aatcttttgc aaaggattta gccatgaaaa
     1741 aactgtctta tgtattgctc gctcttcttg ctctcgccag catttcggct caggccgatg
     1801 tcgtcaccgc aaccaaagcg gcaggcgatt acggtctctg ggcaaaaaaa ctgaatccga
     1861 aatccaccct gtctgcagaa gccggacgcg ccttctacac caagaaggtt gaagttgccg
     1921 gcaaggatct gtcctgcgct gcctgccata ccgacaatcc ggccaatccg ggcaagcaca
     1981 acgaaactgg caagccgatc aagccgatgg cgcctgccgt caatccgcaa cgcttctcca
     2041 acatccagaa atcggcagcc ggcttctcga agcattgcca ggatctgtat ggcaaggatt
     2101 gctcgccaca ggacaagggt gacttcatca cttacctgct gacgatcaag taatcgtcgc
     2161 cacatcaaaa aacccgccac tcggcgggtt ttttttgtat ctgaataccg cttatttttt
     2221 acccgcagcc aatattcccc gcgccatcac ggccagctcc tcttgactca actcggcatg
     2281 aggaggcatg ttcaccctcc cccatacacc cgaaccacca tgagcgatct tgttcgccag
     2341 atagctctcg gcgacatcct tcttgtcttt cttatacttt ttggcgacct ctttccatga
     2401 cggcgcaccc accttgttgc ccggcaaatg gcatgacaga cagcgcgcat cggcgatcgc
     2461 ctgatcgaat gacttcgcct tctcacgctc cgtcttatcg ggcccgacct ccccgccgca
     2521 ccccgacaga acaaccgcgc accagaccag atgaacaatc gcgttgccgc gcaccgatcc
     2581 ccaagcgctt cgcccccggg cggcgttctt tttttcacga ctacttgact gcatccccac
     2641 ctccatggaa tcgatgacaa ctgtagcaag tgctgctgac tttgcccttg acggccttat
     2701 cgtcgacatg acactcgcga cacttctcta tggtcggcat tgaaatatcc gcactcgttt
     2761 ttgaattgat cttgtcatga caactggcgc actcggtcgt gtcatgtttg gcatggggga
     2821 agacggactt cgcatgccag ttgcgattga tccgcaccgg cacaaccttc cagggcaccg
     2881 cgctgtcatc ggacccctcg atctcatggc attcgctgca gctaccgtcc ttgccgaaca
     2941 gcgaaaccgc cgcattcttc tgcgccagat cgctggcaca ggcgaacgtc ccctgcagcg
     3001 aatttcccgg gtcaccattc tgcccgcact ccgccatatt ttcaggcgac tcggccagcc
     3061 attggacgta atagctgcgc atcttgctca tcgcctcgcg caccgagccg tgcggcacct
     3121 cgcctgcgac aggcgcggca aaatccagac tgtggcagcc ggactgctgg caacttctct
     3181 tcatggacat cggttcaaaa tgaataccgg cctcatcggg ccgatggcaa tcgtggcagg
     3241 acatcacggt gtcgccctcc ggacttgaaa caccggcctt gtccagatgc acctgatgcg
     3301 agaatttcag cccaggactc tcggatactt tccgctcctg caacacccgg atcaacggcc
     3361 tgccgtcttt gccgttctga acggcgagac ggaacggcgg atgatctctg gtaaagcgat
     3421 gcacattggc aaccgtcgcc cgtgcattct tcgacttgat atcgccatga caagccacgc
     3481 aacgcgccga gtcatacggc atcaatccgg ccttgccctt gtgatcgaca tgacacgccg
     3541 tgcagcgcat atccttgaac aatgcggcat gcaacgcatc gtcggtcaga tgcttttcca
     3601 ctttcttgtg acatgaggta caggcctcgt cggcaacggc gcgaaagccg cgctgatggc
     3661 acgccgagca ctgctcccca aacgcccgat gcccgctgga catggggccg ggattccacg
     3721 aatcggtcag cgtcacggga agcgccgcct gcgctttatc cagcgaggaa gacagcccgg
     3781 gcagcagcgg gagcagcaga aacaggaaca ggatgccggc ggccagcgcg aagcccaacc
     3841 tgcgcttgct catccccagg gcctccagcg tgagcggctc acgcctgact ggcgtctgtt
     3901 cggcaggtgg tcggatcatc tccaccacca gtgcaatatt cgacccggcc gaagcgggat
     3961 ccacgatcaa cagatagggg ccaatctcca cccgaacgcc ggggcgcagc tcggcccgtt
     4021 gtgccgcgac accctcgacg ctgagcgcgg catcgccctc cgtctcgata taaatcaccc
     4081 cttcttttga ttgcctcagg gtcgcatgat gcagcgcgac ttgctgatcg agcagccgca
     4141 gtttgcaggc catgctgcgg ccgatccgga tggcctctcc ggcaacgata ttttccttgc
     4201 gtacaggcac gccctgccgg ttgcgcgaag actggattaa caggcaatag atcatggtcg
     4261 gctaccagta atacaaaatg gccacgacat gcgcgacgag cgcggccaac aacgcgacag
     4321 tcagcggcac atgcagatac agccagaatt gcattctggc cttcagcatg atttcacgcc
     4381 gtgcgcgcaa caccagactc tccttgcgca gcaatacgcc atagagctcg cgcaacagtt
     4441 tcggctgttc atgcttgatg tatttcggac cgagcttcag catctgccgt gccgccgccg
     4501 tcgtggggca gccccgctgc ctgccgctaa gctgctgcaa caggctgccg cccagccgag
     4561 tgccctggcg cgatttctgc accagggcat tcacctcgtc cggcagagcg agtgcctgca
     4621 cgcgcgccag ctcatcgagt tcatcgatct gcagcagcaa gtcctccagt gtctcctccc
     4681 cctgattcag ggtgatcagg cgcgggtact tcaggtaggc atacaagccg taaaagccac
     4741 tggcgatcac cagcaacatc agcacataag acagcgtatg aacattccat ccgaactgga
     4801 atccggtatg cagtgtcgcc agaacgatca gcgaaccgcc gagattgatg tgcaaggaaa
     4861 gccagccctg caaggtaccg ccgcgactcc aggggtcgcc gggccggggt ctgccgcgat
     4921 cttcccagcg ccgcaggaaa gagttgagcc gcacgctgcc ctgtctgcgc cgctccttcg
     4981 caggactcat ctcccggtgg tggacgcggg gcctgcgtcg cttggcgatt ccgtaccaca
     5041 gcaacaacag cacgatcagc gcggagacga tgcccagcag atagcccagc caggttcctc
     5101 cgtaaggctc gctgccgaga ggcgcgaaca cataagcccc gatggacgct gccatcagca
     5161 agccggatag cttgagatag cgaaaattca tgaagctcag caagcccgag cgatatgcca
     5221 ttttcatgac ctatcctccc ggtctgcgtc gctcgcccag ccgacatggc tgaagaatcc
     5281 ttccggactg accctgaatg cagccccggt cgggcaggca ttgacgcaca gaggcccgcc
     5341 ccgtatccct ttgcacatat cgcacttgac ggcttttttt gacacggaag catccgcctc
     5401 gacgaccgcc ttttttccgg aaccgaacag aatctgccag atgccgccct gctggggctc
     5461 gctttgcacc gccatctgaa tcacgccgta gggacaattt ttctggcaat tgccgcagcc
     5521 gatacagcta tcgctgataa acacctcgcc gttgaccgcg cgctggatcg catccggcgg
     5581 gcaatccttc atgcaatgcg gatgttcgca atggcggcat gaagtcggca aatggatctg
     5641 ctcataggtc atcccggcct cccggtccag ccgggaaatc ccgccatgca catccgcaca
     5701 ggctgtttcg cagttatcgc aacggatgca gcgttcatag tcgatcagca gaacatccgt
     5761 ggcctctccc accccctgcg tcatcaggaa cttgatgagc ccgctgaatg aggtgtcctc
     5821 cggcagcaca tcggcaccga tgcgctccag ataacggctg tccaccttgc cgcgtatgga
     5881 ggcgttgcgc tccatgactg cccggacgcg gccagcctcg agcaacacca catcggtggg
     5941 cgaggctgcg cgcaccgtcg ccagacgcag cgtattcgac accaatgcca tttcaccgac
     6001 atagttcccc gcagaaacat acgcaagcat gacttccttg cccgcgatca gacgcgacac
     6061 cgtcaccgat ccgcgccgga tcaggtacag gccgtcgacc tgatcgcctt ccttaaacag
     6121 cgcttcaccc acggcgtact gtttgaattg ggcatcctga accagatgct cgaactcatg
     6181 ctcgggcaac gctgcatcca gcgagttttg cacgatgcgt ttgagcgcca cctcgtccag
     6241 tcggcgcctc acccccggca ccgcatacaa caatttgagc atggaacggc gcggcgtttc
     6301 gaccagcacg caaccttctc cggccactgc cgtacccgag cggcgacgtc cggacagcag
     6361 ccccatctcg ccgaagaact ccccggcgcc gagccggaag gtcgagaccg caccatcctt
     6421 gccttcggtg tgtatcgtca cgctgccgga aagaatggaa aagaagctgc tgctgtagtc
     6481 gttgcgcttg aacagcacct cgccgcactg cggcacccac agggtgctgt ccagcatgaa
     6541 ctcgcgcagc tgcagggtgg tcagcgttgc aaacagcggc tgattgcgcc ggatcagcgc
     6601 gataccgtca ttgacgctcc gaacttcagg cacgccggag aatttatccc ttaacagcgc
     6661 ctcgtctgcc ggctcgaccg ggttcccctg gatatattcg acgacctcat agccttgatt
     6721 catcgcctgc ttgatcagcg ggtagcccgc cagggcaccc accacataca gcccgggcac
     6781 attcgactcg tattgctccg tcagttgcgg cacggcggtc gagtcgccat tggggaactg
     6841 cacaccaaaa ctctcgacca actggcgcgg cggtttcgcc cccaggcgcg cgatcacgcg
     6901 atggcattcg atgcgtgcca acccctgcga cgtatggacc accagcacca gcggaaaatc
     6961 gcgtcccgtg ctttgctcca cgcgctcggc ccaggtactg acctgcgttt cgattcgacc
     7021 tttggtgact tccgccatca gcaggtcaaa gttggtcttg ctgcaactgg tgaattcttc
     7081 ctgacgattg atgaagatca cccggttgtg ttcggccagc gccagcgcat tttccacgcc
     7141 cgcatcgccc gcgccgatca caacgatggt ttcgccgttg aacgcttcgg ggtcatccag
     7201 ttgatattgc acgccgggca agtcttcgcc cggcacaccc agtttacgga tattgccctg
     7261 cagaccgatg gccaacacca cgaactcagc cgacagcact tctcccgtcg ccaaggtgac
     7321 ctgaaaggcc ccgagcctgc cagaaatgcc ggaaacggcc gcattcaccc gcaggttcac
     7381 ccggtgagca accagctcct cttgccaaca ttgcagaata tgctcgcgaa tcccggcacc
     7441 aaaagtcagg gtgctgcgca gcggcaacac cgcaggctct gccatcacca gcttgcgcct
     7501 ttggtagtga tgcatggtgt tggcggggtg cggcgcggcc tcgagcagga catgagaaac
     7561 gcccagctct gccgctcttg cggcggcaga catccctgcg gggcccgcgc cgatgattac
     7621 aattttgtag tgatcagcca tcgcccggag acatctcgcc gtggatgcta gtcgccgtcg
     7681 aattcctgag tcggcgcgcg gctcaggagt gcggtgatga cgtcggagac gggaatgtgt
     7741 tcgtacagga tgcgatagac cgcctcgcag atcggcatcg cgacatcgag gcgctgcgcc
     7801 agttgatgca cctcgcgcac cgtgtagacg ccctcggcaa catgccccag ccgacgcaat
     7861 atctcgggca ggtcgtgctg ctgcgccagc aacagcccca cctgacgatt gcgcgacaga
     7921 tcgccggtgc aggtcaggat caagtcgccc acgccggaca gcccgctgaa ggtctcgctg
     7981 gtcccgccca gcttcaaccc caatcttgca atctcggcga ggcctcgcgt cagcagtgcg
     8041 gcacgggcgt tgtgtccgag ccccatgcca tcgcagatgc cggcggcaat cgccatcacg
     8101 ttcttgaccg cgccgccgac ctccacgccg accaggtcgc tgctggcata aatgcgcagg
     8161 tgcgcatgat gcagcgccag cgccgtgctg cgcgcgaact cgacatcggt cgaggccagc
     8221 gtcagcgcgg tgggcaggcc gcgtgcaact tccagcgcaa agctcgggcc ggacagaacg
     8281 ccgtaagaaa agcgcgcggg caacacttcg gcgaccacct gatgcggcag caacgaggtc
     8341 tccgcctcga agcccttgca cacccagacc acgccgaagt tgcccgaaga acgggcgagc
     8401 aattgcaggg tggtacggaa cgcagaagtc ggcacggcga cgatggccag ctcggcatca
     8461 gccaaggcgg cgggcagatc cgcgctcagt tcaagatcgg caggcagtgg aataccaggc
     8521 agatagcgct ggttgctgcg tgcgccgcgc atcgcctcga cctgcccggc attgcgcgcc
     8581 cagagcgtga cgcgatggtt ggcagcgagg ctgatggcaa gcgcggttcc ccaagcccct
     8641 gcaccgatga cggtaatctt catatggtaa gaggacgatg catcgcgccc gttagtctgt
     8701 ttatacggca ggaatcaccg gaatcaattg ccccacactt cgctgctgcg cacatagcct
     8761 gtctcgccgt ccagatgacg caccttgatc cagccggtcc ccgtggactc cagacgctcc
     8821 agcgcgacct gctggcgcac cttgaacagg accggcgcgg agaagtccgc agccgcatgc
     8881 acgtcgagca agggcggcaa cgcaaacagg tagcgcttgc tgcccagcgc gcgcgcctcg
     8941 atccagtaca ggccgccgga acggtctcgc accttgaccc aatcctgtaa cgtcaccacc
     9001 tgctcgaacg gcatgtagcg attcaccaca aacagcttct tcgccttgag cgaaggagcg
     9061 tcatagagta tcgcgttgtc ctcggtgaca gaaacgaaat ccacggcgta cgccgctccg
     9121 ccgcacagca gcaggaacag cgcaagcagg cggggcagtt tcatcagtgc gtcgtttccg
     9181 tggcgccaga ctgctgttgc ttctgctgct ggtagagcac gtcaaaattg atcggattca
     9241 gcagcacggg cgcaaaaccg ccgcgcaccg aagcatcgga gaccacttcg cgcaggtacg
     9301 ggaacaggat attcgggcac atcaccgcca gaataggctc cagctctgcg gccggaatat
     9361 tgcggatctg aaagatgcca gcctgcttgg cctcgatcag aaacatcacc ttctcgccga
     9421 tcttggctgt cacggtagac gtgaccgaca cctcgaacat cccctcttcg atggaagccg
     9481 cctgagtgtg caattgcaca tcgatcagcg gattttcacg ctccagaaag atcaatggcg
     9541 cattgggaat ctccagcgag agatccttaa cgtacagctt ctccatgttg aacaatggct
     9601 gagtattttg ttgcgctgct tcggtcatgc tatctcctcc ttaatagtta ttccgccaga
     9661 agcggggcca gctcaccggc ccgatccagc tcagccagat cgtcatatcc gccgacgtgc
     9721 cggtcgccga tatagatctg cggcacggtg cgtcgcccgg tcttctccat catcaccgcg
     9781 cgctgctccg ggtgcaagtc tatgcgtatc ttctcgattc cggtcacgcc cttgcgttgc
     9841 aacaaactct cggcacgcac gcaatacggg cagatttcgg tgcaatacat cacgaccttg
     9901 ggcatcattt ttttaccggc aaactggcct tctgccaggc catcatgccg ccggccagat
     9961 tatagggtga agcgaagcca ttcttggcga gccgcgcgca agcactggcg gagcggttgc
    10021 cgctgcgaca caccaccacg atgggctgct ccttgtattt ttccagctca cccagacgct
    10081 ctgcaagctt gcccagcggg atcagcttgg cattcaatat atggccggat ttgtattcgc
    10141 cctcttcgcg cacatccagc accagggcgc tcttgtgatt gaccagctgc aaggctgcgg
    10201 tgcaatccac ctcctgaatg ccgcggatac gattgccgaa tagcgaccag aacaacatcg
    10261 caccgctaaa cagcgcgatg gaaatcggaa agatgttact agtgatgaac tgcacgagcc
    10321 tgctcctgtt ctttttcgag ggcgaattct agcagagcag cgaaacgttc ggggctcttc
    10381 tccgccaagg aggccagctg ttgacgcaca ctggcgaatt cgcccgggta ggaccatatc
    10441 gcctgttcca tcgccagcat ggcctgttgc ggccgatcgt tcaaggccag cagcagcgac
    10501 tgacgatagg ccgccgtcgc tatcggcaca aaacgcatca ctctggtatt gagcgccagc
    10561 ttgtcctgct tgcgctcttc cgtgacatcg atcatcgaac tcatgaacag ctcggcatag
    10621 ggcgacagca acgagttgcc gtgcacggcg accagtccat cgcgaatacg ctccacggta
    10681 gcatgatcgg ctgccgacgc cggacgcaac cccaacaccc gtcccagctg atgatagctg
    10741 ccgatgaact gcagcagggt gagcagcccc agcaacagca ccgcagccac cgacaggcgg
    10801 ccaaccgcac gcaactccag acggtaacgc gtctcgtcca gcgcccccag caggatagag
    10861 gcgatcccga ggaaataggc gtaccacaac ggatattcca gcaggctatg gatagccagc
    10921 acgcccaatg ccgcataacc ccaccagtgc gcggcatcca gcgaggcccg gcgcgcgcca
    10981 tgcagccaga tagccaatgc cgtgaacagg atcagcaaac cggcgatgcc ggtctcggca
    11041 gccagctgaa acaccagatt atgggcattg ttgtacaagc cctgaatgtt gccctgctgc
    11101 agcacgggca acaattgaaa gtgatgccag gcgaactgcc cgaagcccac gcccagccag
    11161 ggcgactggc tgaatatcag ccaggattcg cgccacagat acagacgaat ccctccgccg
    11221 gcatcctcac cgaacagtcg ctgcacggtg tcggtgctac cgcccgctcc cgccatgaac
    11281 ggcaactgca ccagcagatg catggcgccg aatccggcga tcagcagcag gctgtagcgc
    11341 agcagcggcc gcatcgtctg atcgcgacgc ccccaccacc aggcaagcgt cgccatcatc
    11401 gacagatata gccaggaact gcgcgaaccg ctcagcgtca tcgtcagcaa cagcggcagc
    11461 gccagcagca acgcatagcg gcctttcaat ccgagctgat gcagcagacc gagcgagatc
    11521 agccccagcg cgatgtaatt cgcaaagtgg ttacgctgcg cgagattgcc gaacacatcg
    11581 gcggagacct tcaccaccac caccgaatcg aacggcgtat gccagcgaaa atgctgcaac
    11641 acgccgatcg ccgcgctgag ttcggcgccg atcagcagaa agacggccag cacctgcgcc
    11701 agccgcgcca tgccgaagcg gtcgcgcagc gcggcgccca gcagcatcaa cagcgcggcg
    11761 aacagcagat acagcacata taacagggcc tgatcgaaat aggccaccat tcccaagcca
    11821 agctgcaaca acgccacggc ggccagcgcg atcggcagtt gcgcgatgcg cggaatctcg
    11881 gggcgttgcc agtatgcccg tccgaccagc aaggtcagcg cagcgacacc caataacgcg
    11941 caccaccatt cctgatagaa ggtggtcagc ggatattggt gacggtagtg caaaaacggg
    12001 aagacccaca tcaccccgac cagagccagg ctgatgtctg ccagggttat ttgcaaccgc
    12061 cgcagcggat tgcctagcaa gaacggctct tgataccgaa cagggaaagg atgttggtca
    12121 gcggacagat ctgggtaaaa ccgctctgaa acaggttaaa accgacgaaa ccggtgaacc
    12181 acaaggcttt ctggctcaca aacagcgggc tggcttccac acccagcaac agcgacagca
    12241 tcacaaaaaa accggcgacg atacggacga tacgttctgc attcatgctt cattactcca
    12301 atttaagttg ataaattcta cgggtatttc ttgtacagcg tggcgtaata caacaccggg
    12361 atcacgacca gcgtaagcat ggtactgacc agaatgccga atatcagcga caacgccagg
    12421 ccgttgaaga tcgggtcgtc cagaataaac agcgcgccca gcatcgccgc cagcccggtc
    12481 aggatgatcg gcttggcgcg cgcaccggcg gcattgatca ccgcatgctg cagatccact
    12541 ccgtcgcgca gctgcaggtt aacgaagtcc accagcagga tagaattgcg cacgatgatg
    12601 ccggccagcg cgatcatgcc tatcatcgag gtcgcggtga actgcgaacc gaacagcgca
    12661 tgtcccggca tcacaccgat gatggtgagc ggaatcggcg ccatgatgat caacggcacc
    12721 agataagact tgaactgcgc caccaccagc agatagatca ggatcaggcc gacgccatag
    12781 gcgatgccca tgtcgcggaa ggtctcgaag gtcacctgcc attcgccgtc ccacttcagc
    12841 gcataaccgg catagggatc ggaaggctgc ttgaagaaat agctctccag cacaccgccc
    12901 tgttccagcg ctgcgccgct ggtcttgccg ttgatgtcga acatgccgta cagcgggctg
    12961 tccagc
//
LOCUS       DASUIV010000305         7189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1009063, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000305 DASUIV010000000
VERSION     DASUIV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009063"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1011)
                     /locus_tag="VFW59_10980"
     CDS             complement(4..1011)
                     /locus_tag="VFW59_10980"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5364782.1"
                     /translation="MNYINESKAEPFRTGRRGFLALLSAAVLWTGLPGCRKSPHPLRV
                     AAHIWPGYEFMFLAQREGFLDGGLVTLRETASSTESMKALADGEVDAAALTLDEVLRA
                     RAIGVRLSVVLVFDISAGADVLLGRAPIRQLGEIKGKRIGVEEGALGALMLAEALQAA
                     GLQHGDVQVVPLTIDQHESAWRRGEVDVLVCFPPVSAHLLEAGAVNLFDSRQLPDTIV
                     DVLAVKQSVLEENRDAVQHLVKAHLVARHLMHHNPQDSSFRMARHLNLHANEVLSSFK
                     GLVLADLENNRRLLGGEKPVLSDRARKLSEVMVQAGLLPKADTLNALLRADFLPKDED
                     V"
     gene            complement(1057..3525)
                     /locus_tag="VFW59_10985"
     CDS             complement(1057..3525)
                     /locus_tag="VFW59_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5364783.1"
                     /translation="MTTLATYHVLLVEDDPSDANLARQVLAASTMSLFDVVWVTTIAE
                     TIQALSRDHFDVLLLDLSLPDSSGLETVHTLRQTAGTLPMVVLTGHDDTDFALQTLVA
                     GAQDYLIKGCFDTDSLERTIRYSIERKSLSIHLQQSHDLLHKLSDQVPGLIFQFRLDP
                     DGHMSFPFASKAMLDLYGISPETAMADASAVFAYQHPEDAAGVTASIQESARTLQPWH
                     HEYRIVLPGQDVRWRRGDARPEKQDDGSILWHGFITDITESKMVEARLNLAARVFETT
                     TEAILVTDVDGNIVATNPAFSQITGYSEAEVLGQNPRFLKSDRHTQAFYREFWDALTS
                     KGEWVGEVWNRRKNGEVYAQWESISTIRDKDGKTSRYVAVFSDMSEIKNARDRVEQLS
                     WRDPLTGLANRALFLRQVDQALAGAKRDHRFATVLLLDLDRFKEINEARGLATGDALL
                     QEVAQRFAQLLHADDLLARLDSDEFAVLIHRMHNSPEAAGGEGLAVAGKLLGALREPL
                     VMEGEVMHIDASVGIAILPDPGQVTAGEVLRQADMAMAKAKSEGRARIMFFETMMGES
                     IRERFQLEAELRVAIAESQLRLYLQPQVDARGLQVGAEALVRWQHPTRGLLPPGLFIP
                     LAESSDLIVALDRWMLSAVLGLLVQLDAEGRTLRLSVNISPRHFRQTDFVDEIKRQLR
                     VSGADPSHLVLELTEGVVIGDIGDVIAKMTTLSTLGIHFSIDDFGTGYSSLAYIKRLP
                     IHELKIDKSFIQDSTTDPNDAALVETILSIAQHLHFQVVAEGVETQAQADFLNERGSI
                     IHQGYLYGRPEPVEKWLMKLGGEQ"
     gene            3701..5545
                     /locus_tag="VFW59_10990"
     CDS             3701..5545
                     /locus_tag="VFW59_10990"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5364784.1"
                     /translation="MPAMNLLSKPLLRIIALFILLAVAETVWLHSLLPLENRLSDLFV
                     REQAQALTPDPQIVIVDIDDSSLARMQEVAGNWPWPRSVHGELVRSIAAQHPAAIVFD
                     ILFSERDLYRPESDRLFNEALEAGDNLYFPIIRRDPVLDAQGAPLKQIAPMLGLTRTD
                     RADPEARVALLPPLAIDPRHWRGGTINFKEDADGVGRRYQLYTDVRGWLIPSMSARVA
                     ADLGYGVPQQDDMILAWRGTARSFRHIAYADLYEDLGREHPLRPQDEFTGKIVIVGTA
                     ATGLHDVRVTPVASLYPGVEILATALDNLKNARMMQAAPVWFAPLLALGLLCALGAAF
                     LRGINVLRSGLWLAAASALLLQGSHFATGQRYLLPVLTPLLLAWGCYFSCALAEYLRE
                     RRTREQAVQLFSRFVNPHVVKELIAHGGLNRTGESRQITVLFSDIRGFTTLSERHTPQ
                     EIVALLNRYFTLQVEVVFRHGGSLDKFIGDAIMAFWGAPLDDARHAQNAVAAALEMAE
                     VLQRFKQELGESGLEFDVGIGLHSGPAVVGLIGSEQRREYTAIGDTVNLASRIEGLTK
                     GVSRILVSRDTMELCGDEFDFKSFGSYKVKGREQEVELFAPTKKEMTS"
     gene            5542..6030
                     /locus_tag="VFW59_10995"
     CDS             5542..6030
                     /locus_tag="VFW59_10995"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5364785.1"
                     /translation="MKSLLAFCLAFTAFTACAGEAAYTVRPTEVKAKPFSDAETLARL
                     PEKSPVEVLARKSSWMQIKRDGTTGWVKMLNLRLGDAAAKKSGDSGLGALFNVAATGG
                     SGSTVSTGVRGLSEEKLKNPQPNPQELRELKRYATSKQEARNFGGSGKLATHQIDYLP
                     AK"
     gene            6042..6905
                     /locus_tag="VFW59_11000"
     CDS             6042..6905
                     /locus_tag="VFW59_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5364786.1"
                     /translation="MKKPLVSVLLLCCLSANAQAFDLNDLGSVLGTLQKAQQANADID
                     ESKEVELGSGIASNLLGAAPLLKNQAVQRYVNHIGMWLALQTERPNLPWQFGVLDDDD
                     INAFAAPGGYIFVTRGLLAQMKSEAELAGVLAHEMSHVLRRHYLQALKKGARTELLAE
                     FAGKAMQSQGADPNLTKLVNAGTEVYARGLDKSDEFEADRMGVVIATRAGYDPYGLPA
                     VLQTLQGLNAQDSSLALLFKTHPALPERLNLLDKMMTGQFDDFENQPDNAPRFMSVMN
                     KTGILIHTGKR"
     gene            6944..>7189
                     /locus_tag="VFW59_11005"
     CDS             6944..>7189
                     /locus_tag="VFW59_11005"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEX5364787.1"
                     /translation="MKHALTISLTLLCAASAAYAEPGEWWEIASKTEMPGMPFSMPAT
                     TTRICVAKGAADDPRQSVQDKECKMTDIKKSGNKTSWK"
BASE COUNT         1496 a         2328 c         2091 g         1274 t
ORIGIN      
        1 agattaaaca tcttcatcct tgggcaagaa gtcagcgcgc aataaggcat tgagcgtgtc
       61 cgcctttggt aacagcccgg cttgcaccat gacctcgctc agcttgcgcg cgcggtccga
      121 caacaccggc ttttccccgc cgagcaggcg gcggttattc tccagatcgg ccagcaccag
      181 ccccttgaac gaggacagca cctcgttggc gtggagattg agatgacgcg ccatgcggaa
      241 gctggaatct tgcgggttgt gatgcatcag gtggcgcgcg accaaatgag ccttgacgag
      301 atgctgcacg gcatcgcgat tttcttccag cacgctctgt tttaccgcca gcacatccac
      361 gatggtgtcc ggcaattggc ggctgtcgaa caggttcact gcgcccgcct caagcaggtg
      421 tgcggaaacg ggcggaaaac acaccaacac gtcgacctcg ccgcgccgcc aggccgattc
      481 gtgctgatct atggtaagcg gcaccacctg cacgtcacca tgctgcaagc ccgccgcctg
      541 caatgcctcg gccagcatca gtgcgcccag cgcgccctct tccactccga tgcgcttgcc
      601 cttgatttcg ccgagttggc ggatgggcgc acgccccagc aatacatcgg caccggcgga
      661 aatatcgaac accagcacca cagacaaccg caccccaatc gcgcgcgcgc gcaatacctc
      721 atcaagcgtg agtgcggccg catcgacttc gccgtcggcg agcgccttca tggattcggt
      781 agaggatgcc gtttcgcgca acgtcaccag ccccccatcc agaaagccct cgcgctgcgc
      841 cagaaacatg aattcgtaac cgggccagat atgggcggcc acccgcagcg gatggggact
      901 cttgcggcat cctggcaacc cggtccacag gacagcggca ctgagcaacg ccagaaagcc
      961 acgacggcct gtccggaaag gctcagcctt cgattcattg atatagttca ttcgaacctc
     1021 ccgatcgttc gggtcgaaac attagctcac gacgatttac tgctcgccac ccagtttcat
     1081 cagccatttc tctaccggct ccggtcgccc gtacaaatag ccctgatgaa taatggaacc
     1141 tcgctcattg aggaaatccg cctgcgcctg tgtctccacg ccctcggcca cgacctgaaa
     1201 atgcaaatgc tgggcgatgg acaggatggt ctcgaccagc gccgcatcgt tcggatcggt
     1261 ggtggaatcc tgaataaagc tcttgtcgat cttcaattca tggataggca ggcgcttgat
     1321 ataggccagg ctggaatagc cggtaccgaa gtcatcgatg gaaaagtgta tgcccagcgt
     1381 cgacagggtc gtcatcttcg cgatcacatc gccgatgtcg ccgatcacca ccccctccgt
     1441 cagctccagc accagatggc tgggatcggc gccgctcacc cgcagctggc gtttgatctc
     1501 atcgacgaaa tcggtctgcc gaaaatgacg cggactgata ttgaccgaca ggcgtaaggt
     1561 gcggccttcg gcatccagct gcaccagcaa accgagaacg gcggacagca tccagcggtc
     1621 gagtgcgacg atcaagtcgg aactctcggc cagcggaatg aacagaccgg gcggaagcag
     1681 accgcgcgtc gggtgctgcc agcgcaccag cgcttccgcc cccacttgca ggccccgggc
     1741 atcgacctgc ggttgcagat agaggcgcaa ctggctctcg gcgatcgcaa cccgcagttc
     1801 ggcttcgagc tggaagcgct cccgtatcga ctcgcccatc atcgtttcaa agaacatgat
     1861 gcgcgcccgg ccttccgatt tcgcttttgc catcgccata tccgcctgac gcaggacctc
     1921 cccggccgtc acctgcccgg ggtccggaag gatggcgata ccgacactgg catcgatgtg
     1981 catgacctca ccttccatga cgaggggttc gcgcagcgcc cccagcagct ttcccgccac
     2041 ggccagccct tcgccccctg ccgcttcggg cgagttatgc attctatgaa tcagcacagc
     2101 gaactcgtcc gaatcgagac gggccaacag gtcgtcggca tgcaacagct gggcgaatcg
     2161 ctgcgccacc tcttgcaaca aggcgtcgcc cgtggccaat ccgcgcgcct cgttgatttc
     2221 cttgaaacgg tcgagatcga gcagcaacac cgtagcgaaa cgatggtccc gctttgcacc
     2281 tgccagcgcc tgatccacct ggcgcagaaa cagcgcgcga ttcgccaaac cggtgagcgg
     2341 gtcgcgccag gacaattgtt cgaccctgtc ccgcgcattt ttgatctcgc tcatatcgga
     2401 aaacacggcg acataacgag aggtttttcc gtctttatcc cggatcgtgc tgatcgattc
     2461 ccattgcgca tacacctcgc cgttcttgcg ccggttccag acctcgccga cccactctcc
     2521 cttgctggtg agcgcatccc agaactcgcg atagaaggcc tgcgtatgac ggtctgattt
     2581 gagaaaacgc ggattctgcc ccaacacctc ggcctcgctg tatccggtga tctgcgaaaa
     2641 agccggattg gtcgcgacga tattgccgtc gacatcggtc accagtatcg cctcggtcgt
     2701 cgtctcaaag acccgggccg cgaggttcag gcgagcctcg accatcttgc tttcggtgat
     2761 gtcggtgatg aagccatgcc acaggatgct gccgtcatcc tgcttttccg gccttgcatc
     2821 ccccctgcgc cagcgcacgt cctggcccgg caacacaatg cggtattcgt gatgccaggg
     2881 ctgcagtgtc cgtgccgatt cctgtatcga ggcggtgacg cctgccgcat cctccggatg
     2941 ctgataagcg aagacagccg aggcatccgc catcgccgtc tccggcgaga tgccatacag
     3001 atcgagcatc gccttgctgg cgaacgggaa gctcatatgc ccgtcaggat cgagtcggaa
     3061 ctggaatatc agccccggaa cctgatcgga gagtttatgc agcaagtcgt gactctgctg
     3121 caagtggata gacagcgact tgcgctctat cgaataacga atggtgcgct cgagcgagtc
     3181 ggtatcgaaa cagcccttga tcagataatc ctgcgcccca gccacgagcg tctgcaaggc
     3241 gaaatcggtg tcatcgtgcc ccgtcagcac gaccatcggg agggttcccg cagtctgacg
     3301 cagcgtatgg accgtctcga gtccggagga gtcaggcagc gacaaatcca gcaacagcac
     3361 gtcgaaatgg tcgcgtgaaa gcgcctgtat cgtctcggca atcgtggtca cccacacgac
     3421 atcgaacagc gacatcgtgc tggcggccag aacctgacgc gccagattcg catcgctggg
     3481 atcatcttcg accagcaata catgataggt agccaatgta gtcaacgaaa caccccggaa
     3541 taaccgcgcg ctcggcgctt gcagacagac gcaatgaagc ccctgtggct cgcatgaact
     3601 ctagcagaaa taacggcgcg agacgggctc aaattttgag ctcccatccg ccagaggaca
     3661 tcctctcgcc atctgtccat gccccgagaa tgggtttaac atgcccgcca tgaacctgct
     3721 atccaaaccc ctgctgcgca tcatcgccct gttcatcttg ctggcggtcg ccgagacggt
     3781 ctggctgcac agcttgctgc cgctggaaaa ccgtctttcc gacctgttcg tgcgcgaaca
     3841 ggcgcaggcg ctgacacccg atccgcagat cgtcatcgtg gacatcgacg acagcagcct
     3901 cgcacgcatg caggaggtcg cgggaaactg gccctggcca cgctcggtac acggcgaact
     3961 ggtgcgcagc atcgcggcac aacacccggc ggccatcgtg ttcgacatcc tgttcagcga
     4021 acgcgacctg taccgccccg agagcgaccg cctgttcaac gaggcgcttg aggctggcga
     4081 caacctctac tttccgatca tccggcgcga tccggtgctg gatgcccagg gtgcgccgct
     4141 gaagcagatc gccccgatgc tcgggctgac tcgcaccgac cgggccgatc cggaggcacg
     4201 ggtcgcgctg ctgccgccgc tggccatcga cccgcgtcac tggcgcggcg gcaccatcaa
     4261 cttcaaggaa gacgcagatg gcgtggggcg gcgctaccag ctctataccg acgtgcgcgg
     4321 ctggctgatc ccgtcgatgt cggcgcgcgt ggccgccgac ctgggctacg gggtaccgca
     4381 gcaggacgac atgattctcg cctggcgcgg cactgcccgc agcttccggc acatagccta
     4441 tgccgatctt tacgaagatc tggggcgcga acatccgctg cgtccgcagg acgaattcac
     4501 cggcaagatc gtcatcgtcg gcaccgcagc caccggcctg catgacgtgc gcgtcacacc
     4561 tgtcgccagc ctgtatccgg gcgtggagat actggcgacc gcgctggaca atctgaagaa
     4621 cgcacgcatg atgcaggcag cgcccgtctg gttcgccccg ctgctcgcac tcggcctgct
     4681 gtgcgcactg ggcgctgcct tcctgcgcgg catcaatgtg ctgcgctccg gcctgtggct
     4741 ggccgcagcc tcggcgctgc tgctgcaggg cagccacttc gcaaccggac agcgctacct
     4801 gctgcccgtg ctcaccccgt tgctgctggc ctggggctgt tacttctcct gcgcgctggc
     4861 cgaatatctg cgcgaacgcc gcacgcgcga acaggcggtg caactgttca gccgtttcgt
     4921 caatccgcat gtggtcaagg agctgatcgc ccatggcggc ctcaaccgca ccggcgagag
     4981 ccgccagatc acggtgctgt tctccgacat ccgcggcttc accacgctgt cggaacgcca
     5041 cacgccgcag gaaatcgtgg cactgctcaa ccgctatttc accctgcagg tcgaggtggt
     5101 cttccgccat ggcggatcgc tggacaagtt catcggcgac gccatcatgg ccttctgggg
     5161 cgcgccgctg gacgatgcgc gacacgcgca gaacgcggta gcggccgcgc tggaaatggc
     5221 cgaggtgctg caacgcttca aacaggagct tggcgagagc ggcctcgaat tcgatgtcgg
     5281 catcggcctg cattccggcc ccgccgtggt cggcctgatc ggttcggaac agcggcgcga
     5341 atacaccgcc atcggcgaca cggtgaatct cgccagccgc atcgaaggcc tgaccaaggg
     5401 cgtgtcgcgc atcctggtgt cgcgcgacac catggagctg tgcggcgacg agtttgactt
     5461 caagtcgttc ggttcgtata aagtcaaggg gcgggagcag gaagtcgaac tatttgcacc
     5521 aaccaaaaag gagatgacct catgaaaagt ttgctcgcat tctgcctggc attcaccgcc
     5581 ttcaccgctt gcgcaggcga agcggcgtac accgtgcgtc ccaccgaggt gaaagccaaa
     5641 ccgttcagcg atgccgagac tctggccaga ctgccggaga agtctccggt cgaggtgctc
     5701 gcaagaaaat caagctggat gcagatcaaa cgcgacggca cgacaggctg ggtgaagatg
     5761 ctgaacctgc gcctgggcga tgcggccgcg aaaaaatccg gcgacagcgg gctgggagcg
     5821 ctgttcaatg tcgccgccac cggcggcagc ggcagcaccg tctccaccgg cgtgcgcggg
     5881 ctctccgaag aaaagctgaa gaacccgcaa cccaatccgc aggaattaag ggaattaaaa
     5941 cgctatgcga ccagcaaaca ggaggcgcgc aacttcggcg gctcgggcaa actggccacg
     6001 caccagatcg attatctgcc agcgaaataa gggagcgaaa aatgaaaaaa ccactcgtat
     6061 ccgttctgct gctgtgctgc ctcagcgcca atgcgcaagc cttcgatctg aacgatctgg
     6121 gcagcgtatt gggcaccctg caaaaagccc agcaggccaa cgccgacatc gatgaatcga
     6181 aagaggtcga actgggcagc ggcatcgcca gcaacctgct gggcgccgca ccgctgctga
     6241 agaaccaggc cgtacaaaga tatgtgaacc acatcggcat gtggctggcg ctgcaaaccg
     6301 aacgccccaa tctgccctgg caattcggcg tactggacga tgacgacatc aacgctttcg
     6361 ccgcacccgg cggctatatc ttcgtcaccc gtggcctgct cgcgcaaatg aagagcgagg
     6421 ccgagctggc gggcgtgctg gcgcacgaga tgtcgcatgt tctgcgcaga cattatctgc
     6481 aagcgctaaa gaaaggcgcg cgcaccgagc tgctggccga gttcgcagga aaggcgatgc
     6541 agagtcaggg ggccgacccg aatctgacca agctggtcaa cgcaggcacc gaggtctacg
     6601 cccgaggact ggacaagagc gatgaattcg aggctgaccg catgggcgtg gtgatcgcca
     6661 cccgtgccgg ttacgaccct tacggcctgc ccgccgtgtt gcaaaccctg cagggattga
     6721 acgcgcagga ttccagtctg gcgctgctgt tcaagaccca cccggccctg cccgagcgcc
     6781 tgaacctgct ggacaaaatg atgaccgggc agttcgacga tttcgagaac cagcctgaca
     6841 atgcgccgcg cttcatgagc gtgatgaaca agaccggcat actgatccac acgggcaaac
     6901 gttaacgccg ccgattcgca cttcaactcg atagggagtc atcatgaaac acgcactgac
     6961 gatcagcctc accctgctat gcgctgcttc tgccgcttat gccgaaccgg gcgaatggtg
     7021 ggaaatcgcc agcaagaccg agatgcccgg catgcctttc agcatgccgg ccacgaccac
     7081 ccggatctgc gttgcaaagg gtgctgccga cgatccgcgc cagtcggtgc aggacaagga
     7141 atgcaagatg accgacatca agaaatccgg caacaagacg agctggaag
//
LOCUS       DASUIV010000306         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1103266, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000306 DASUIV010000000
VERSION     DASUIV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103266"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1982)
                     /locus_tag="VFW59_11010"
     CDS             complement(<1..1982)
                     /locus_tag="VFW59_11010"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEX5364788.1"
                     /translation="MINEDHLEQLALTWFQDCGWEYCYGPDIAPDGTAPERADYKQVV
                     LVERLLDALGRLNPGVPESVLDEVAHRVGKLHEPSLLQSNRSFHELLLDGVAVEVEVD
                     GVKRGDRVRLMDFEHAENNRFLVVNQFTLQGIKQPRRPDLVCFINGLPISVIELKNPA
                     AEQADIWSAFNQLETYKAEISDLFVFNEALVISDGLLARVGSLTSDRERFLPWRTLRS
                     ENDKPLLEFELEKVVRGFFAPDLLLDYLRYFILYEQGEDGIVKKIAGYHQFHAVREAV
                     RATVIAATGPLPNAAELERATYGKEVKPGSRKGGIVWHTQGSGKSISMVCFVGKLMQQ
                     PQMQNPTIVVVTDRNDLDGQLFQTFTGARALLKETPQQAEDRDALRTLLDGRPSGGII
                     FTTMQKFALGKDEDRFPELSKRTNIVVIADEAHRSQYGFNAVLDKKTGKYKYGFAKHL
                     RDALPHATFVGFTGTPVESDDKDTRAVFGDYVSIYDIQDAVDDGATVPIYYESRLAKL
                     DINQAQIEQLNEDVEEVFEDEENAATREAAKSKWAALEKLVGAEPRILQVAADIVQHF
                     EARTAATEGKGMIVGMSREICAHLYNAIVALRPEWHSNDPAQGAIKVVMTGSAADKEL
                     LQPHIYNKSTKTQLEKRFKNPKDPLKLVIVRDMWLTG"
     gene            complement(1975..>2570)
                     /locus_tag="VFW59_11015"
     CDS             complement(1975..>2570)
                     /locus_tag="VFW59_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364789.1"
                     /translation="TEHTHDLTLAFQRLVRLGVLESSGHGKGTVYHLPGQNLPTPDQA
                     FGESDVISGAPGASSESSSGHNDMSSGHNERLQSNSSRHDDGHLLVKGLNKPLIDNLK
                     LLPEALRQELMSVAEEARTKPRLAPDEMTAILIRLCENAYLTQQVLAELVNRKPEALR
                     KNILKPLVDKGLLALAFPTVPTHPQQAYTANKGRRDD"
BASE COUNT          514 a          824 c          656 g          576 t
ORIGIN      
        1 ccggtcagcc acatgtcgcg cacgatcacc agcttcaatg gatctttcgg attcttgaag
       61 cgtttttcca gttgtgtttt tgtgctcttg ttatagatgt gcggctgcaa caattcctta
      121 tccgccgccg aaccggtcat caccaccttg atcgcgccct gtgccggatc gttgctgtgc
      181 cactcgggac gcaaggccac aatggcattg tagagatgcg cacaaatctc gcgactcatc
      241 cccacgatca tgccctttcc ttcggttgcc gccgtgcgcg cctcgaaatg ctgcacgata
      301 tccgccgcca cctgcaggat gcgcggctcc gcccccacca gcttctccag cgcggcccac
      361 ttggacttgg ccgcctcgcg cgtcgcggcg ttctcctcgt cctcgaacac ttcctcaaca
      421 tcctcgttta gctgctcaat ctgcgcctga ttgatgtcca gcttggcgag gcggctctcg
      481 taatagatcg gcaccgttgc tccgtcgtcc accgcatcct gaatatcgta gatgctgacg
      541 taatcgccga acaccgcccg cgtgtccttg tcgtcgctct ccaccggcgt accggtgaag
      601 cccacaaagg tcgcgtgcgg caacgcatcg cgcagatgtt tggcgaagcc atatttgtac
      661 ttgccggttt tcttgtccag tacagcattg aaaccgtact gtgaacggtg cgcctcatcg
      721 gcgatcacca cgatgttggt gcgcttactc aactcgggga aacgatcctc atccttgcct
      781 agtgcgaact tttgcatggt cgtgaaaata atcccgccgg acggccgccc atccagcagc
      841 gtgcgcaacg catcgcgatc ctccgcctgt tgcggcgttt ctttcagcag cgcacgcgcc
      901 ccggtaaaag tctggaacaa ctgcccgtcc agatcgttgc ggtcggtcac caccacgatg
      961 gtcggattct gcatttgcgg ctgctgcatc aacttgccga cgaaacacac catcgaaatg
     1021 ctcttgcccg aaccctgcgt gtgccaaacg atgccgccct tgcgcgaacc gggcttcact
     1081 tccttgccgt aagtcgcacg ctctaattcc gccgcattgg gcaacggccc cgtggccgca
     1141 atcaccgtcg cccgcaccgc ctcgcgcacc gcatggaact ggtgataccc cgcaatcttc
     1201 ttgacgatgc cgtcctcgcc ctgctcgtac agaatgaaat agcgcaagta atccagcagc
     1261 agatcgggcg cgaagaaacc gcgcaccacc ttctccaact caaattccag caatggcttg
     1321 tcgttttcgc tgcgtagcgt gcgccagggc aagaagcgtt cgcgatctga cgtaagcgaa
     1381 cccacgcgcg ccagcaggcc atctgaaatc accagcgcct cgttgaatac aaacagatcg
     1441 gaaatctccg ccttgtaggt ttccagttga ttgaacgcag accagatatc cgcctgctcg
     1501 gctgcggggt tcttcagttc gatcactgaa ataggcaggc cgttgataaa acacaccaaa
     1561 tcgggacggc gcggctgctt gataccctgc agggtgaact gattcaccac cagaaagcgg
     1621 ttgttttcgg catgttcgaa atccatcagc cgcacgcggt cgccgcgctt caccccgtca
     1681 acttccacct ccaccgccac gccgtccagc aacaactcat gaaagctgcg gttgctctgg
     1741 agcagcgaag gctcgtgcag cttgcccacc cgatgcgcca cctcgtccag cactgattcc
     1801 ggcacacccg ggttcaatcg gcccaacgca tcaagcaagc gctcgaccag caccacctgc
     1861 ttgtaatccg cacgctcggg ggctgtgcca tccggcgcaa tgtccgggcc atagcaatat
     1921 tcccagccgc agtcctgaaa ccatgtcagg gcgagttgtt cgaggtggtc ttcgttaatc
     1981 atcacgccta cccttattgg cggtatacgc ctgttgtgga tgcgttggta ctgtcgggaa
     2041 tgccaaagcc aacagcccct tatcgaccaa cggcttcaaa atattttttc tcagcgcctc
     2101 aggtttgcga tttaccaact cggccagcac ttgttgagtc agataagcat tttcacatag
     2161 ccttatgagg atggcggtca tttcatccgg agccagccgt ggcttcgttc tggcttcttc
     2221 agctacggac attagctcct gacgcagggc ttccggcagc agcttgaggt tatctatcaa
     2281 cggcttgttc aggcctttga ccagcagatg tccatcatca tggcgcgagg aattggactg
     2341 caaccgctcg ttatgtccgg agctcatgtc gttatgtccg gagctcgatt ccgatgaagc
     2401 gcctggagct ccggatataa catcgctctc gccaaacgcc tggtcaggag tggggagatt
     2461 ctgtcctggg agatggtaaa ccgtaccttt gccatgcccg ctggattcca gaacccccaa
     2521 cctgacaagc cgctggaaag caagcgtgag atcatgggta tgctccgtgg
//
LOCUS       DASUIV010000307         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1197803, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000307 DASUIV010000000
VERSION     DASUIV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197803"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VFW59_11020"
     CDS             <1..1344
                     /locus_tag="VFW59_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5364790.1"
                     /translation="ISENVQKLEDSVKSEEGIKLINTLKEARAAYVKQLDRFIETNKS
                     GDGEGARKIFFGEMVDETVIYRKAIDTLIQDQEGLMKKAGDDASKAYDEARNLMIALA
                     IGAALLAIGIAFWVTRSITRPLNEAVSVADALAAGDLTVRIKVDSTDETGMLLTSMQN
                     MVGKLSQIISEVRSAAENLTSASEEVSSTSQSMSQATNEQAASVEETSASVEQMGASI
                     NQNSENARVTDGMATQAAKQATDGGEAVKETVTAMKQIAGKIGIIDDIAYQTNLLALN
                     AAIEAARAGEHGKGFAVVAAEVRKLAERSQVAAQEIGELASGSVSKAEQAGKLLDEIV
                     PAISKTSDLVQEITAASEEQSSGAAQINTAMNQLNQITQQNASATEELAATAEEMSSQ
                     AEQLQQLMGFFRVQGGAVAVKHSAAPAKSAALPAKAAPKLLASRAAQDESEFVKF"
     gene            1486..2034
                     /locus_tag="VFW59_11025"
     CDS             1486..2034
                     /locus_tag="VFW59_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5364791.1"
                     /translation="MNAIATINQSLPALANNATQEEQQRQYLTFMLSGEIFAIGILRI
                     KEIIEYGQLTEVPRMPDFVRGVINLRGAVVPVIDLGARFGKQATAVSRRTCIVIIEVQ
                     HEDEQQVVGVMVDAVNEVLDIAPAEIEPAPSFGAKIRADFIRGMGKVEGKFVIILNVD
                     HVLSLDEMSTLAGANAGTDGAA"
     gene            2235..>3411
                     /locus_tag="VFW59_11030"
     CDS             2235..>3411
                     /locus_tag="VFW59_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5364792.1"
                     /translation="MFDKLKVGTRLSGAFGVVIAIFLAVMLLLSIQLGQVKRGANQIE
                     GETLPFILVVEDMALSVSQVQQFLTDVSATHNRAGYRDAEAAAQRYLADSGKFKAMYR
                     QENDTAALHKVEEIEARFRVFYATGKDMAEVYITGGVVAGNGVMERFDAESTALDRSL
                     SAFREQQVSEAVGITEAMNASVSKLNRQMLGGTLVALIFALLLGLWITRSLTRQLGGE
                     PAYAAETVGKIAAGDLTVQVQTRPGDRTSMLAAIKVMTEKLAQTIGEVRLAADSLGSS
                     SEEISSTSQSMSQATNEQAASVEETSASVEQMGASISQNSENARVTDGMAAQAAKQAN
                     DGGEAVKETVAAMKQIAGKIGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEV
                     "
BASE COUNT          766 a          940 c         1117 g          588 t
ORIGIN      
        1 atctccgaga acgtccagaa gcttgaagac tcggtcaagt cggaagaggg catcaagctg
       61 ataaataccc tgaaagaggc gcgcgcggcc tatgtcaagc aactcgatcg ctttatcgaa
      121 accaataaat ccggcgatgg cgaaggcgca cgcaagatat tcttcggcga gatggtcgac
      181 gagacggtta tttacaggaa agctatcgat accttgattc aggatcaaga agggttgatg
      241 aagaaggctg gagatgatgc atcgaaagcc tatgacgaag cgcgcaatct gatgatcgcg
      301 ctggccatcg gtgccgcgct gctggctatc ggcatcgcct tctgggtcac ccgttccatt
      361 acccgtccgt tgaatgaggc cgtgagcgtt gccgatgcgc tggccgcagg ggatcttacc
      421 gtgaggatca aggtcgactc cacggacgag accggcatgt tgctgacctc gatgcaaaac
      481 atggtgggca agttgtcgca gatcatcagc gaagtgcgca gcgcggcgga gaatttgacc
      541 agtgcgtccg aagaagtcag ttccacctcg cagagcatga gccaggccac caacgagcag
      601 gccgcctcgg tggaagagac ttccgcctcg gtcgaacaga tgggtgcttc gatcaaccag
      661 aactccgaga acgccagggt caccgacggc atggcgactc aggccgccaa gcaggccacc
      721 gacggcggcg aggccgtgaa agaaaccgtc accgcgatga aacagatcgc gggcaagatc
      781 ggcatcatcg acgacatcgc ctaccagacc aatctgctgg cactgaacgc ggcgatcgaa
      841 gcggcgcgtg caggcgaaca cggcaagggc ttcgcggtgg tcgcggccga agtgcgcaag
      901 ctggcggaac gttcgcaagt ggccgcacag gagatcggcg aactggccag cggcagcgtg
      961 agcaaggcgg agcaggctgg caagttgctg gacgagatcg ttcccgcgat cagcaagacc
     1021 tccgatctgg tgcaggagat caccgccgca tccgaggagc aaagctcggg tgcagcgcag
     1081 atcaacaccg cgatgaacca gctcaaccag atcacccagc agaacgccag tgccacggaa
     1141 gagctggcgg caacggcgga agaaatgtcg tcgcaggccg agcaactcca gcaactgatg
     1201 ggattcttca gggtgcaagg cggagctgtc gcagtgaagc atagcgctgc cccggcgaaa
     1261 tctgccgcac tgcctgccaa agccgcacct aaattgctgg cttcgcgtgc cgcacaagac
     1321 gaatccgaat tcgtgaagtt ctaagcggca gcttcgcagg atgggtggag gcgatagccg
     1381 ataaccaatg acttggttgg cgttgtttgc caatttagtc agatggctat gcaaggctac
     1441 ccatcctgca ggctcaggta attaaagatt tattaaggag gcgtaatgaa cgcaatcgca
     1501 acaatcaatc aatcgctccc tgccctcgcg aacaatgcga cgcaggaaga gcagcagcgt
     1561 cagtacctga cgttcatgct gagcggcgag atattcgcca tcggcattct gcgcatcaag
     1621 gagatcatcg agtacgggca gctgaccgaa gtgccgcgca tgccggattt cgttcgcggc
     1681 gtgatcaacc tgcgcggagc ggtggtgccg gtgatcgacc tcggcgcgcg cttcggcaag
     1741 caggcgacgg cggtgtcgcg ccgcacctgc atcgtcatca tcgaagttca gcatgaagac
     1801 gagcagcagg tggtgggcgt gatggtggac gcggtgaacg aagtgctgga catcgcaccc
     1861 gccgagatcg agcccgcccc gtcgttcggc gccaagatcc gagccgattt catccgaggc
     1921 atgggcaagg tggagggcaa gttcgtcatc atcctcaacg tcgatcacgt gctgtcgctg
     1981 gacgagatgt cgactctggc cggagccaat gccgggacgg atggcgcggc ttaagggcaa
     2041 tcgcggctag ttcccaaggg gtatgccggt gggtacaccg ctgcttgcac agcgacccct
     2101 ccgcaggcaa atggtgaggt ggtagccgct cccacagagg agcggttttc gttacaaccg
     2161 agtggctgcg caagccgccg catgtcgagc tttcgggtga tacaaagaat ctaataacga
     2221 tcggggaggg atttatgttc gacaagctta aagtgggtac ccgtttgagc ggcgcctttg
     2281 gcgtggtgat cgcgatcttt ctggccgtga tgctgttgct gtccattcag ttggggcagg
     2341 tcaagcgtgg ggcgaatcaa atcgagggtg aaaccttgcc attcatcttg gtggtggagg
     2401 atatggccct gagcgtgtct caggtgcagc agtttctgac cgatgtttcc gccacccaca
     2461 atcgggctgg ctatcgggat gcggaagccg ccgcccaacg ctatttggcc gactcgggaa
     2521 aattcaaggc gatgtaccgc caggaaaacg ataccgccgc actgcacaag gtcgaggaga
     2581 tcgaggcccg gttccgggtg ttctatgcca cgggcaagga catggccgag gtttacatca
     2641 ccggtggggt ggtggccgga aatggcgtga tggagaggtt cgacgcggag agcaccgcgc
     2701 tggatcgctc cttgagtgcg ttccgcgaac agcaggtgag cgaggcggtc ggcatcacgg
     2761 aggcgatgaa tgcgtcggtg agcaagctta atcgccagat gttgggtgga acgctggtgg
     2821 cgttgatttt tgcgctcttg ctcggcctct ggatcacccg ttccctgacg cggcagctgg
     2881 gcggggaacc cgcctatgcg gcggagactg taggcaagat cgccgccggt gacctcaccg
     2941 tgcaggtgca gacccggccc ggcgatcgca ccagcatgct ggccgcgatc aaggtcatga
     3001 ccgagaaact cgcgcaaacg ataggtgaag tgcgcctcgc ggcggatagt ctgggcagct
     3061 cctccgagga aatcagcagc acttcgcaga gcatgagtca ggccaccaac gagcaggccg
     3121 cctcggtgga agagacttcc gcctcggtcg agcagatggg cgcttcgatc agccagaact
     3181 ccgagaacgc cagggtcacc gacggcatgg cagcgcaagc cgccaagcag gcgaatgacg
     3241 gaggcgaggc ggtgaaagaa acggtcgccg cgatgaagca gatcgcgggc aagatcggca
     3301 tcatcgacga catcgcctac cagaccaatc tgctggcact gaacgcggcg atcgaagcgg
     3361 cgcgtgcggg cgaacacggc aagggtttcg cggtggtcgc agccgaggtg c
//
LOCUS       DASUIV010000308         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1291780, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000308 DASUIV010000000
VERSION     DASUIV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291780"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFW59_11035"
     CDS             complement(<1..1065)
                     /locus_tag="VFW59_11035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5364793.1"
                     /translation="MSYKIPNHLETLRLRVLDRMAALLESQTRSKIFSASVSELVRDV
                     HQEVIGDLRDAAAGIDDPALATAMRRAADMCTRLDGSLKLLLDERSRQWYRNGDYVQG
                     LMKEFNATLGQLSSTLIEKDLFERQSRVLEHIVLSHENISQWKEFVQEILSEFHSIFP
                     FNFFYIAFAEEHEFSLYLYYLGEYSQEVRDAARHMLAKQMLASLGQPADAPLDIEEFV
                     IDQGGHIGSIDEVQMIAVSVPEHAPNLAGMLGVAYVSAGKLPPQEESVIRAILAVMVM
                     VVGSSKALSRTLSELEYYSVHDPLTGLYNRRHFNAMLEYEIGRSERHKHEFSVLLLDL
                     DDFKDINDTYGHATGDEALRR"
     gene            complement(1073..1846)
                     /locus_tag="VFW59_11040"
     CDS             complement(1073..1846)
                     /locus_tag="VFW59_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5364794.1"
                     /translation="MRNIPLFEAENHRFVLLNESDPGEEEGVRSNQYLVMHEDAGVLL
                     DPGGFGVMPRVLAEMLRYLPPEKIRGIVLSHQDPDIVGGLSTWLELTQCPVYVSRIWM
                     RFLPHYGLKNVSQFVGVPDEGMPLTVQPGFDLKIVPAHFLHSEGQINVYDPVSKILFS
                     GDIGAAMLPMDRDEAFVDDFRAHLPYIASFHRRYMCSNKAIRCWLDTIAGLEIDMIAP
                     QHGPVYRGQAVREFLDWLKDLQCGIDLMQHGGHFPLDFA"
     gene            complement(1931..>2397)
                     /locus_tag="VFW59_11045"
     CDS             complement(1931..>2397)
                     /locus_tag="VFW59_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5364795.1"
                     /translation="GKVPALELDDGSGLYDSPVICEYLDGKNPVAHLIPQSKRIEVKR
                     WEALADGICDATVAIVMEGRRAPEKQDDTIVARQKLKVERGLKVLAHDLGDSKWCLGE
                     ALSLADIAVGCTLGYLCLRMPELAWRKEYPNLARLYDQLMERPSFKNTVPPT"
BASE COUNT          479 a          743 c          716 g          459 t
ORIGIN      
        1 gcggcgcaag gcttcgtcgc cggtggcgtg accgtaggtg tcgttgatgt ccttgaaatc
       61 gtccaggtcc agcaacagga cggaaaactc gtgcttatgc cgctcggagc gtccgatctc
      121 atactccagc atggcgttga aatggcggcg attgtagagg ccggtcagcg ggtcgtgcac
      181 ggagtagtac tccaactcgg acagcgtgcg cgacaaggcc ttgctggaac ccaccaccat
      241 gaccatcacg gcgaggatgg cgcggatcac gctttcttcc tgcggcggca gcttgccggc
      301 cgacacatag gccacgccca gcatcccggc caggttgggc gcatgctccg gcaccgacac
      361 cgcgatcatt tgcacttcgt ctatcgaacc gatatggccg ccctggtcga tcacgaattc
      421 ctcgatgtct agcggcgcat cggccggttg gccgaggctg gccagcatct gtttcgccag
      481 catgtggcgc gccgcatcgc gcacctcctg cgagtattcg ccgaggtaat acaggtagag
      541 cgagaactca tgctcctcgg caaaggcgat gtaaaagaag ttgaacggaa agatgctgtg
      601 gaattcggac aggatttcct gcacgaactc tttccattgc gagatgttct cgtgcgacag
      661 cacgatgtgc tccagcaccc ggctctgccg ctcgaacagg tctttctcga tcagcgtgga
      721 agacaactgc cccagcgtgg cgttgaactc cttcatcaag ccctgcacgt aatcgccgtt
      781 gcgataccac tggcggcttc gctcgtcgag cagcagtttc aggctgccat ccagccgcgt
      841 acacatatcc gccgcccggc gcatcgccgt ggcaagcgcc ggatcgtcga ttcccgccgc
      901 cgcatcgcgc aggtcgccga tgacctcctg atgcacgtcg cgcaccagtt cggacacgct
      961 ggcactgaat atcttggaac gggtctggct ctccagcagc gccgccatgc ggtcgagcac
     1021 gcgcaggcgc agcgtctcga ggtggttggg tatcttgtaa ctcatggacg cctcaagcga
     1081 agtcaagcgg gaagtgcccg ccgtgctgca tgaggtcgat gccgcattgc aggtccttga
     1141 gccaatcgag gaactcgcgc accgcttggc cgcgatacac cggcccgtgc tgcggcgcga
     1201 tcatgtcgat ctccagcccg gcaatagtgt ccagccagca gcggattgcc ttgttggagc
     1261 acatgtagcg gcgatgaaac gacgcgatgt acggcaggtg cgcccggaaa tcgtcgacaa
     1321 acgcctcgtc cctatccatg ggcagcatgg cggcgccgat gtcgccactg aacaggattt
     1381 tcgagacggg gtcgtaaacg ttgatctgcc cctcggaatg cagaaagtgc gctggaacga
     1441 ttttcaaatc gaagccgggc tgcaccgtca gcggcatgcc ttcatcgggc acgccgacga
     1501 attgggacac gttcttcaaa ccgtaatgcg gcaaaaagcg catccagatg cgggaaacgt
     1561 acaccgggca ctgggtcagt tccagccagg tggagaggcc gccgacgatg tcggggtctt
     1621 gatgggagag cacgatgccg cgaattttct cgggcggcag gtagcgcagc atttcggcca
     1681 gcacgcgcgg catcacgccg aaaccgcctg gatcgagcaa cacgcccgca tcctcgtgca
     1741 tcaccagata ctggttggag cgaacgcctt cttcttcccc cggatcgctc tcgttcagca
     1801 ggacgaagcg gtgattttcc gcttcaaaca agggtatgtt gcgcatgccc gactcccccg
     1861 caaattataa aaataattgc ggacaatgct atcttgttta aggagcaggc gcaagcaaag
     1921 gagaaacggt ttatgtcggg ggaacggtat tcttgaagga cgggcgttcc atcaattgat
     1981 cgtacaaccg ggcaaggttg ggatattcct tgcgccaagc cagctccggc atgcgcaagc
     2041 acaagtaacc caaggtgcag ccgacggcga tgtcggccag gctcaaggcc tcgcccagac
     2101 accacttgct gtcgcccaag tcgtgcgcca gcaccttcag gccgcgctcg accttgagct
     2161 tctgccgggc gacgatggtg tcgtcctgct tttccggcgc gcggcggcct tccatcacaa
     2221 tcgccacggt ggcatcgcag atgccgtccg ccagagcttc ccaacgctta acctcgatgc
     2281 gcttggactg cgggatcagg tgcgccacgg ggttcttgcc gtccaggtat tcgcagatca
     2341 ccggcgaatc gtagaggccg ctgccgtcgt ccaactccag cgccggcacc ttgccca
//
LOCUS       DASUIV010000309         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1292020, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000309 DASUIV010000000
VERSION     DASUIV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292020"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFW59_11050"
     CDS             complement(<1..562)
                     /locus_tag="VFW59_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364796.1"
                     /translation="MMMDKYSYPIEGGKIKTRALEAHIVDHCNLRCEGCCSLSPFLPE
                     SFTGPADLAADLALVSQVLSPTFLKLVGGEPLLHPDLIECLVVAKQSSVAPVLYVTTN
                     GLLLPKMPDDFWRLIDGLTISLYPSPALPQKTISLVLERAEQFGVHLNWKRQDVFVQM
                     DRTHPSDDAELDASVFSVCWLRRRCHL"
     gene            complement(569..1135)
                     /locus_tag="VFW59_11055"
     CDS             complement(569..1135)
                     /locus_tag="VFW59_11055"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclin-dependent kinase inhibitor 3 family
                     protein"
                     /protein_id="HEX5364797.1"
                     /translation="MSPTITSFDSPLRIDSYILSDTGGQIGMTICPGKNRPGSISGDW
                     DRDLALDLQVVKDWGAEIVLTLLEDFEFAQVGVEALGPQVKALHMHWLHLPIPDKHAP
                     TASFAQQWQTAGPLVHACLQRGGKILIHCMGGIGRTGTVAAQILMERGMAPDEAMVAI
                     RTARRGAIETEVQEMYLLALRLPSVHIP"
     gene            complement(1146..2165)
                     /locus_tag="VFW59_11060"
     CDS             complement(1146..2165)
                     /locus_tag="VFW59_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamide-nucleotide
                     adenylyltransferase/Nudix hydroxylase"
                     /protein_id="HEX5364798.1"
                     /translation="MSQFDAAILIGRFQPFHIGHAALLAKALETSPRVVVLLGSAFSA
                     RNAKNPFSWEERAAMIACSLRESDRERVSFLPMRDYYDDVRWATTAATLIQQTLSGKA
                     RVALIGYLKDASSQYLNRFPDWDFVGMERQGDIDATALRRIWFEGEDPEVTSALLTPL
                     LPPAVLHYMRGWAALPMFANLRDEHFAVEESRKVWGSGPFITVDAVVKASEQVLLVKR
                     GRAPGKGTWALPGGFLDGRERVLQGAIRELREETGFGLLDASLEAALAGTAVFDHPDR
                     STRGRTITHAHFFDLKHTRPPEVAGADDAAEAKWFSVVELAGMEGEFFEDHFNILNHF
                     LNLAC"
     gene            complement(2176..>3069)
                     /gene="nadE"
                     /locus_tag="VFW59_11065"
     CDS             complement(2176..>3069)
                     /gene="nadE"
                     /locus_tag="VFW59_11065"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX5364799.1"
                     /translation="EEGLTAMEFYRRQIVLGLRDYARRCGFTRAVVGSSGGIDSALTL
                     ALAAEALGADNVVGITMPSKFSSAGSVDDSVALCRNLDVRLIEHPIRDLVAAYGEGFA
                     AAFDTPLQGLTLENLQARVRGTILMEYSNAFGHLLLTTGNKSEISVGYCTLYGDTNGG
                     LGLIGDLYKTEVFELCRHINACAGRELIPYAIIDKEPSAELAPGQRDTDSLPPYPVLD
                     AILKQLIEGERLGEDERAAVAAAFAEHQASDQGRALIARIKRMIARNEYKRRQAPPIL
                     RLRGRAFGSGRQMPIAAKYDF"
BASE COUNT          632 a          932 c          943 g          562 t
ORIGIN      
        1 ccagatgaca gcgtcgcctc aaccagcaca cggaaaaaac actggcatcc agctccgcat
       61 catcagaggg gtgagtcctg tccatctgca cgaaaacatc ctgccgcttc cagttgagat
      121 gcaccccgaa ctgctccgcc ctctcaagga ccagagaaat cgttttttgc gggagtgccg
      181 gcgacgggta cagcgagatc gtcaagccat cgatgagacg ccagaagtcg tctggcattt
      241 tcggtagcaa cagtccattg gtggtcacgt acagcacagg agcaacgctg gactgcttcg
      301 ccaccaccag gcactcgatc aggtccgggt ggagcaaggg ctcgccgccg accagcttga
      361 gaaatgtcgg cgacaagacc tgcgagacca atgccagatc agcagccaga tctgccggtc
      421 cggtgaacga ttccggcaag aatggcgaca aggaacaaca accctcacaa cgcagattgc
      481 agtgatccac gatgtgcgct tccagggcgc gggtcttgat cttcccgcct tcgatggggt
      541 agctgtattt atccatcatc atacggatct acgggatgtg cacggatggc agacgcagcg
      601 ccagcaaata catttcctgc acttcggttt caatggcgcc gcgccgggcc gtccgaatcg
      661 ccaccatcgc ctcatcaggc gccatgcccc gctccatcag gatctgtgca gcgacggtgc
      721 ccgtacggcc gatgccgccc atgcaatgga tgagtatctt gccgccgcgc tgcaagcagg
      781 cgtgcaccag cggtccggcc gtttgccatt gctgcgcaaa gcttgcggtc ggtgcatgtt
      841 tgtccggaat gggcaaatgc agccagtgca tatgcagcgc tttgacctgc ggcccaagcg
      901 cctcgacacc gacctgcgcg aactcaaaat cctccagcaa ggtcagcaca atctctgcgc
      961 cccagtcttt caccacctga aggtcgaggg cgagatcacg atcccagtcg ccagagattg
     1021 agccggggcg gttcttgcca gggcagattg tcatgccgat ttggccgccg gtatccgaca
     1081 ggatgtaact gtcaatgcgc aacggggagt caaaactggt gatggttggg gacatggatg
     1141 gaatctcagc aggccaggtt caggaagtgg ttgaggatgt tgaaatggtc ctcaaagaac
     1201 tccccctcca tgccggcgag ttctacgacg gaaaaccact tcgcctcggc agcatcatct
     1261 gcgccggcta cttcgggcgg gcgggtgtgc ttcaggtcga aaaagtgggc atgggtgatg
     1321 gtccggccgc gcgtgctgcg atccggatgg tcgaagaccg ccgtgccggc cagcgccgct
     1381 tcgagactgg catcgagcag accaaagccg gtttcctcgc gcagctcgcg gatcgcgccc
     1441 tgcagcacgc gctcgcgccc atcgagaaac cctccgggca gcgcccaggt gcccttgccg
     1501 ggcgcacggc cgcgcttgac taacaagacc tgttccgaag ctttgaccac ggcatcgaca
     1561 gtaatgaagg gaccactacc ccataccttg cggctctctt cgacggcgaa atgttcgtcg
     1621 cgcagattcg caaacatcgg caaagcggcc cagccgcgca tgtagtgcag cacagccggc
     1681 ggcagcagcg gcgtcagcaa ggcgcttgtt acttcggggt cttcgccctc gaaccagatg
     1741 cggcgcagcg cggtggcgtc gatgtcaccc tgacgctcca tcccgacgaa gtcccaatca
     1801 gggaaacggt tgaggtactg gctggaggcg tccttgagat aaccgatcag tgctacgcga
     1861 gccttgccgc tgagagtctg ttggatcagc gtggctgcag tcgtggccca gcggacatca
     1921 tcgtagtagt cgcgcatagg cagaaagctc acgcgctcac ggtcagactc tctcagcgag
     1981 caagcaatca tggcagcacg ctcttcccaa ctgaacggat tcttggcatt gcgggcagaa
     2041 aaggcggaac cgagcagcac gactacgcgc ggtgaagttt ccagcgcttt ggcgagcaag
     2101 gccgcgtggc cgatgtggaa gggctggaag cgaccgatca gtatggcggc atcgaattgg
     2161 ctcatctgtg tctcctcaga aatcatactt ggccgcaatt ggcatctgac ggccgctgcc
     2221 gaaggcgcgt ccgcgcagcc gcagaatggg cggggcctgg cggcgcttgt actcgttgcg
     2281 ggcgatcatg cgcttgatgc gcgcgatcag tgcacggcct tggtcgctgg cttggtgctc
     2341 ggcaaaggca gcagccacgg ctgcacgttc gtcctcaccc aagcgttcgc cctcgatcag
     2401 ctgcttgagg atcgcgtcca gcacagggta aggcggcagg ctgtcggtat cgcgctggcc
     2461 cggtgctaac tcggctgaag gctccttgtc gatgattgcg tacggaatca gctcacgccc
     2521 tgcgcaggcg ttgatgtggc gacacagctc gaagacctcg gtcttgtaga ggtcgccgat
     2581 caggcccaga ccgccgttgg tgtcgccgta cagcgtgcaa tagcccaccg aaatctcact
     2641 cttgttgccg gtggtgagca gcaggtggcc gaaggcgttg gagtactcca tcaggatggt
     2701 gccacgcaca cgtgcctgca gattctccag tgtgagcccc tgcaagggag tgtcgaaagc
     2761 ggcggcgaag ccctcaccgt aagcggcgac tagatcgcgg atcggatgct caatcaggcg
     2821 cacatcgagg ttgcgacaga gcgcgacaga atcatcgaca ctgccggcgc tggagaattt
     2881 tgaaggcatg gtgatgccga cgacgttatc ggctcccagt gcttcagcgg caagtgccaa
     2941 ggtcaatgcg ctgtcgatgc cccccgagga gccgaccacg gcacgcgtga aaccacagcg
     3001 gcgcgcgtaa tcgcgcaggc ctagcacgat ctggcggcga tagaactcca ttgcagtcag
     3061 gccttcttc
//
LOCUS       DASUIV010000310         9650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_174853, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000310 DASUIV010000000
VERSION     DASUIV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174853"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFW59_11070"
     CDS             <1..555
                     /locus_tag="VFW59_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5364800.1"
                     /translation="FVKNRYIGRTFIMPGQAMRKKSVRQKLNAIGVEFKGKNVLLVDD
                     SIVRGTTSREIVQMARDAGALKVYFASAAPPVRFPNVYGIDMPSRTELIATGRSDEEI
                     CNEIGADGIIYQDLDDLKAAVRKANPAIVDFDASCFDGNYITGDITAEYLDAVEAARG
                     ASAESKAADDDQMELNLAMSVSSM"
     gene            744..1922
                     /locus_tag="VFW59_11075"
     CDS             744..1922
                     /locus_tag="VFW59_11075"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX5364801.1"
                     /translation="MSDENSYQPETLAVRAGSAQSQFHEHSEALFLTSSFTFDNAAQA
                     ARRFIGEEPGNIYARFTNPTVTMFEERLAAMEGAEQCVATASGMSAILACVMGVLKAG
                     DHIVASRSLFGATVNLFNNIIKKFGVETTYVSATDVAEWQAAVRPNTKLFFLETPSNP
                     LTEISDIAAISVVAKGCGALLAVDNCFCTPILQRPLELGADIVIHSATKYLDGQGRVL
                     GGAVLGSRKNMEGVYGFLRTAGPTMSAFNAWIFLKGMETLKIRMDAHSAGALELATWL
                     EAQPNVARVFYPGLPSHPQHELAMRQQKTGGGIVSFEVQGGKDAAWRVIDNTKMLSIT
                     ANLGDTRTTITHPASTTHARITPEARAAAGISDGLIRIAVGLEALVDIKNDLARGLNG
                     "
     gene            1924..2415
                     /locus_tag="VFW59_11080"
     CDS             1924..2415
                     /locus_tag="VFW59_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX5364802.1"
                     /translation="MDVLAAQIGGLLKAHGLMLATAESCTGGGVGQAITEVAGSSAWF
                     ERGFVTYSNLSKQQMLDVNESTLRQHGAVSEAVVREMVAGALRHSGAQVALAVSGIAG
                     PDGGTPDKPVGTVWFAWGLRDGLVHARVHHLDGNRSEVRSRAVRIALQGIVDLLGQRT
                     EFA"
     gene            2496..3548
                     /gene="recA"
                     /locus_tag="VFW59_11085"
     CDS             2496..3548
                     /gene="recA"
                     /locus_tag="VFW59_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5364803.1"
                     /translation="MDDNKSKALAAALSQIEKQFGKGSIMRLGEHDVARDIQVVSTGS
                     LGLDIALGVGGLPRGRVIEIYGPESSGKTTLTLQVIAEMQKLGGTAAFIDAEHALDPQ
                     YAQKLGVKVEDLLISQPDNGEQALEIVDMLVRSGSVDIVVVDSVAALTPKAEIEGEMG
                     DAQMGLHARLMSQALRKLTANINRSNTMVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRMDIRRTGAIKKGDEVIGNETRVKVVKNKVAPPFKEANFDILYGEGISREGEVIE
                     LGVQHKLVEKSGAWYAYKGEKIGQGKDNAREYLKANPAIAFEIENKVREALGIALLDG
                     GEKAKGKAKADTKAAE"
     gene            3562..4041
                     /gene="recX"
                     /locus_tag="VFW59_11090"
     CDS             3562..4041
                     /gene="recX"
                     /locus_tag="VFW59_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEX5364804.1"
                     /translation="MRKRPELSLRTRAMQYLARREYSRAELRGKLLPHVQLDEEFDSA
                     SAPDLDALLDDLTQRGWLSDERAATQWVHAKRSRLGTQRITHELRQKGISEELIAEVL
                     PELKESELATAREVWQKKFGTPPEDAKEKARQMRFLQSRGFSMDVIFSVMRAGGAED"
     gene            4044..4286
                     /locus_tag="VFW59_11095"
     CDS             4044..4286
                     /locus_tag="VFW59_11095"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEX5364805.1"
                     /translation="MPVILRYKGYTFFFFSNEGNPREPLHVHVRRAEASVKFWVEPQV
                     CVAESYGLNGQELNELAKVVADNRQLIERTWHEFFG"
     gene            4270..4524
                     /locus_tag="VFW59_11100"
     CDS             4270..4524
                     /locus_tag="VFW59_11100"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX5364806.1"
                     /translation="MSSLAKAVSFDAATMWVDFTDGRKLGVPLAYFPRLLSATSAQRE
                     HYEISGGGSGLHWDEIDEDISVENLLLGIGDRTRNHLMAA"
     gene            4618..5010
                     /locus_tag="VFW59_11105"
     CDS             4618..5010
                     /locus_tag="VFW59_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HEX5364807.1"
                     /translation="MSSLSRKFIAVLLLIWLPLSGTSALAASLSMQAPHSACHEAQME
                     EHGAMDQHEHGTMDQDASHDQTAAQDQSDSSCTTCGVCHLACSGYLAVPEVATLNMPQ
                     GLAAVTPYLFSFHSITSTPLDPPPLVRC"
     gene            5139..5513
                     /locus_tag="VFW59_11110"
     CDS             5139..5513
                     /locus_tag="VFW59_11110"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX5364808.1"
                     /translation="MDLNKTSTTLGGIRIVGMLRTLLLCTSLVLMSGSAMAEQFKHGA
                     TQGSTSSMQLTDAVVKKIDRKHAKVTLQHGDIPDVMPAMTMAYRLKDARLMDGLHVGD
                     SVRFALEKSGDDYIVTHLEVAR"
     gene            5571..6830
                     /locus_tag="VFW59_11115"
     CDS             5571..6830
                     /locus_tag="VFW59_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5364809.1"
                     /translation="MNRFIAWCLVGCLLSGFACAAEAPLGADVGGLLSYAREHNPELA
                     AARYEAEAVQQRTESAGALPDPVLRTELMDITNQGTKSASLNPSQVGGTRYLLMQTVP
                     WFGKRGLQRDVAEAQAAQADGQAAATWTELAGQIKTAYANYYYLSRSEQLAQQTLKLL
                     VALEHIAHERYANGLATQQEVIRAQVEQTALRGELIELQNGVHHQHSRLNALLSRPGM
                     AVLAEPAQLRPIPAQARLDYAVLADRLRARNPQLLMADARSNEAVKSRDLTYVNRYPG
                     FTLGVAPTQSGNRVKSWDLMVEMNIPLQQGSRRAQEREADALLSAAESRKQALQDRML
                     NMLSEGLSGLESARRTEALTTTSLLPQAELTYQSALAGYENGRVGFAMLLEAQQQILK
                     AKQQRLKAQLDAQLRLAEIENLLGEEL"
     gene            6827..8344
                     /locus_tag="VFW59_11120"
     CDS             6827..8344
                     /locus_tag="VFW59_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5364810.1"
                     /translation="MKNIYLIFIAVLLGVAAGSYWLGLKGRIEDRGSRVAAKSERPVL
                     YYRNPMGLPDTSPVPKKDAMGMDYVPVYEGESAADGAIDIGSDKVQKLGVKSEPAALR
                     VLEHKLRAVGRIEIDERRSYTVAPKFEGWVERLYVNTTGQMVVKGQPLFEVYSPELVS
                     AQREHALAVQGVADMKDADGEARDSMKRLADASAARLRNWDVGGASGGKTVTFHAPVS
                     GIVLEKKAVQGVRFMPGEMLYQIADLSSVWVMADVAEQDIASIKLGDAVRVGVDAYPA
                     QRFDGKVDFIYPTLNSATRTVSLRVVIPNSQGLLKPSMFASVELATGKSDKVLSVPIS
                     AVIDSGTRQVVLVRLAEGRFAPREVKTGRRGDAYVEVLAGIAEGEQVVTSANFLIDAE
                     SNLRAALGGLGGVTAAAEPSSQPAVPDKKSASVGHQGQGKFNEINEDGSVSITHEPIA
                     SLGWPGMTMDFALANSALTKGIQPGSAIRFEIVERAKGEWVITQLQAAPQKHEGH"
     gene            8346..>9650
                     /locus_tag="VFW59_11125"
     CDS             8346..>9650
                     /locus_tag="VFW59_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5364811.1"
                     /translation="MLSKIIDWSARNTFLVLLATLFVVLAGIYAIIRTPLDALPDLSD
                     VQVIVYTEYAGQAPQVVEDQVTYPLTTAMLAVPKSKVVRGFSFFGASFVYVIFEDGTD
                     IYWARSRVLEYLNSIAGRMPKGVSPQLGPDATGVGWVYQYAVLSEKHNLAELRTMQDW
                     YLRYQLTKAQGVAEVASIGGFVQQYQVTVDPVKLRAYGIPLSRVSQVIRESNRDVGGR
                     VVEMAETEYMVRGRGYLHGVGDIESLVVKAERGTPVLLRDIARVELGPDERRGLTDLD
                     GEGEVVSGIAMARYGQNALEVIANIKAKIAEITPGLPEGVSIRAVYDRSELIQRAIAT
                     LKKTLFEEGLIVAVVCMVFLMHARSALVAIVMLPIGVLIAFIAMRALGLNSNIMSLGG
                     IAIAIGAMVDAAIVMIENAHKHLERMQEGESRFDAMLTACKEV"
BASE COUNT         1958 a         2703 c         3178 g         1811 t
ORIGIN      
        1 tttgtgaaga accgctacat cggtcgcacc ttcatcatgc cggggcaggc gatgcgcaag
       61 aagtcggtgc gccagaaact caacgcgatc ggcgtcgagt tcaagggcaa gaacgtgctg
      121 ctggtggacg actccatcgt gcgcggcacc accagccgcg agatcgtgca gatggcacgc
      181 gatgcgggcg cgctcaaggt gtatttcgct tccgccgcac cgccggtgcg tttccccaat
      241 gtatacggca tcgacatgcc gagccgcacg gagttgatcg ccaccggtcg ttccgacgaa
      301 gagatctgta acgagatcgg tgcggacggc atcatctatc aggatctgga tgatctcaag
      361 gcggcggtgc gcaaggccaa tccggccatc gtcgatttcg atgcctcctg ttttgacggc
      421 aattacatca ccggcgatat cactgcagaa tacctggatg ctgtcgaggc ggcgcggggg
      481 gcgagtgcgg agagcaaggc ggcggatgac gaccagatgg agctgaatct ggcgatgagc
      541 gtcagctcga tgtaacaaaa caggattgag gattgaggat tgaggataca acagggcgtt
      601 gaggttgacg cttggaaaga ccgggtgata tttttcaact gtgccgattt gaggaacagc
      661 ttacgggcgc atgataaata cagctaaagg gtaacccggt ttagctcaca agcttaccgg
      721 gttttttgtt ttcaggagat accatgtcgg atgagaattc ataccagccg gaaacactgg
      781 cggtacgcgc aggcagcgcg cagagccagt tccatgagca cagcgaggcg ctgttcctga
      841 cctccagctt taccttcgat aacgccgcgc aggcagccag acgtttcatc ggcgaggagc
      901 ccggcaacat ctacgcgcgc ttcaccaatc cgaccgtcac catgttcgag gagcgtctcg
      961 cggcgatgga aggggcggag caatgcgtcg cgaccgcttc cggcatgtcg gcgatcctgg
     1021 cgtgcgtgat gggcgtgctg aaggcgggcg accatatcgt cgcttcgcgc agcctgttcg
     1081 gcgcgacggt caatctgttc aacaacatca tcaagaagtt cggggtcgag accacctacg
     1141 tgtccgcgac cgatgtcgcc gaatggcagg ctgcggtgcg cccgaacacg aaactgttct
     1201 ttctggagac gccgtccaac ccgctgaccg aaatatccga catcgcggcg atctctgtcg
     1261 tcgcgaaggg ttgcggcgcg ctgctggcgg tggataactg cttctgcacg cccatcctgc
     1321 agcgtccgct ggaactgggc gcggacatcg tgattcactc cgcgaccaag tatctggacg
     1381 gacaaggcag ggtgctgggc ggcgcggtgc tgggcagcag gaagaacatg gaaggcgtgt
     1441 acggtttcct gcgcactgcg ggcccgacca tgagcgcatt caacgcctgg atattcctga
     1501 agggcatgga gacgctgaag atccgcatgg atgcacactc ggcgggcgcg ctggaactgg
     1561 cgacctggct ggaggcccag cccaatgtgg cgcgggtgtt ttaccccggc ctgccgtcgc
     1621 atccgcagca tgagttggcg atgcgccagc agaagaccgg cggcggcatc gtgtcgttcg
     1681 aggtgcaggg cgggaaggat gcggcctggc gtgtgatcga caacacgaag atgctgtcga
     1741 tcaccgcgaa cctcggcgac acgcgcacga ccatcactca tccggcgagc accacccatg
     1801 cccgtatcac gcccgaagcg cgtgccgcag cgggcatcag cgacggcctg atccgcatcg
     1861 cggtcggact ggaggcgctg gtggatatca agaacgatct ggcacgcggc ttgaacggct
     1921 gatatggatg tcttggccgc acagataggc ggcttgctca aggcgcacgg cctgatgctc
     1981 gcgactgccg agtcttgcac cggcggcggt gtcgggcagg caatcaccga ggtggcggga
     2041 agttcggcct ggttcgagcg cggtttcgtc acctattcga acctttccaa gcagcagatg
     2101 ctggacgtga acgaatcgac gttgcggcag catggtgcgg tctctgaggc ggtggtgcgc
     2161 gagatggttg cgggcgcatt gcgccacagc ggcgcgcagg tggcgctggc ggtcagcggc
     2221 atcgccgggc ccgacggcgg cacgcccgac aagccggtcg gcacggtgtg gttcgcctgg
     2281 gggctgcgcg atggactggt ccatgcacgg gtgcatcatc tggacggcaa tcgttcggaa
     2341 gtccgttcca gagccgtgcg catcgcgttg cagggcatcg tcgatttgct ggggcagcgc
     2401 accgaattcg cctgaaataa aaaagagaac gaacgttctg tacaaattaa aaacgatgtg
     2461 ccacaatccg ccatcatttt tcaaagggga tcatcatgga tgacaacaag agcaaggcac
     2521 tcgcggcggc attgagccag atcgaaaaac aattcggcaa gggctcgatc atgcgtctgg
     2581 gcgagcacga cgtggcgcgc gacattcagg tggtatccac cggttcgctg gggctggaca
     2641 tcgcgctggg cgtcggcggc ctgccgcgcg gtcgcgtgat cgagatctac ggccccgagt
     2701 cgtccggcaa gaccacgctg acgctgcaag tgatcgccga gatgcagaag ctgggcggca
     2761 cggcggcgtt tatcgacgcg gaacacgcgc tcgatccgca atacgcgcaa aagctcggcg
     2821 tgaaggtgga agacctgctg atctcgcagc cagacaacgg cgaacaggca ctcgagatcg
     2881 tcgatatgct ggtgcgttcc ggttcggtgg acatcgtggt ggttgactcc gtcgccgcgc
     2941 tgacgccgaa ggccgagatc gaaggcgaga tgggcgacgc acagatgggc ctgcatgccc
     3001 gcctgatgtc gcaagccttg cgcaaactca cggccaacat caatcgttcc aacaccatgg
     3061 tcatcttcat caaccagatc cgcatgaaga tcggcgtgat gttcggcaat cccgagacca
     3121 ccaccggcgg caacgcgctg aagttctacg cctcggtgcg tatggacatc cgccgcaccg
     3181 gcgcgatcaa gaagggcgac gaggtgatcg gcaacgagac ccgcgtcaag gtggtgaaga
     3241 acaaggttgc cccgccgttc aaggaagcca acttcgacat tctctacggg gaaggcatct
     3301 cgcgcgaagg cgaggtcatc gagctgggcg tgcagcacaa gctggtcgaa aagtccggcg
     3361 catggtacgc ctacaagggc gagaagatcg gccaaggcaa ggacaacgct cgcgagtacc
     3421 tgaaggctaa cccggccatc gccttcgaga tcgagaacaa ggtgcgcgag gcgctgggca
     3481 tcgcgttgct cgacggcggc gaaaaagcca agggcaaggc gaaggctgat acgaaagccg
     3541 ccgaataggc cggacacccc ggtgaggaaa aggccagagc taagtctgcg cacccgcgct
     3601 atgcagtatc tggcgcgccg cgaatacagc cgagccgagt tgcgcggcaa gctgttgccg
     3661 catgtgcagc ttgacgagga attcgattcc gcctcggctc ccgacctcga tgccttgctg
     3721 gacgatctga cgcagcgcgg ctggctgtcc gacgaacgtg ccgcgacgca atgggtgcat
     3781 gccaagcgca gccgtctggg cacccagcgc atcacgcacg aactccgtca gaaaggcatc
     3841 tccgaggaac tgatcgccga ggtgttgccg gagctgaagg agtccgagtt ggcgaccgca
     3901 cgcgaggtgt ggcaaaagaa attcggcacg ccgcccgagg atgcgaaaga gaaggcgcgg
     3961 cagatgcgct tcctgcaatc gcgcggtttt tcgatggatg tgattttcag cgtgatgcgc
     4021 gcaggtggtg cggaggattg acgatgcctg ttatattgcg ctacaaaggc tacacgtttt
     4081 tcttcttctc caacgaaggc aatccaagag agccgctgca tgtacatgtg agaagggcag
     4141 aggcctcggt gaaattctgg gtcgaaccgc aagtctgtgt agcggaaagc tacggcttga
     4201 atggtcagga attgaatgag cttgcgaagg tggttgctga taaccgccaa ttgatagaaa
     4261 ggacatggca tgagttcttt ggctaaagcg gtaagtttcg atgcggccac gatgtgggtg
     4321 gactttaccg atggccgcaa gctgggtgtt ccgctggcct attttccacg cctgctgagt
     4381 gccacatccg cgcaacgcga acattacgaa atcagcggtg gcggcagcgg tttgcactgg
     4441 gatgagatcg atgaagacat cagcgtcgag aacctgttgc tgggtatagg cgacagaacc
     4501 agaaaccacc tgatggcagc ttgaggtttc ttgcgaggct gccactgttt cgcaagattc
     4561 tttccacagg tggattcatt gtttgacaga acccgtcgag tttgccaagc tgtgcccatg
     4621 tcgtctcttt ccagaaaatt cattgccgtg ctgttgctga tctggttgcc gttgtccggc
     4681 actagcgcac tggcggcatc gctgtccatg caggcaccgc acagtgcctg ccatgaggcg
     4741 cagatggaag agcatggcgc catggatcaa cacgagcatg gcaccatgga tcaagacgca
     4801 tcgcacgatc agacggcagc acaagaccag tcggattcat cctgcaccac ctgcggtgtc
     4861 tgccatctgg catgcagcgg ctatctggct gtgcccgagg tcgctacttt gaacatgccg
     4921 caagggctgg ctgcagtcac gccttatctg ttctcgtttc actccatcac ttccacaccg
     4981 cttgaccctc cgccgctcgt ccgctgctga gcagggacga gtccgtctgt cggtttcata
     5041 agctgatcca gccggttttg ccggcacccc atctcgtccc gtatcgttcg gcagtcgcat
     5101 tgcgtctgct cgctcattga ttctcgagga ttcgaaacat ggacttgaat aaaaccagta
     5161 ccacccttgg tggcatccgt atcgtcggca tgttgcgcac gctgttgctt tgcacgagtc
     5221 tcgttctgat gtctggttct gcgatggcgg aacagtttaa gcatggcgcg acgcagggca
     5281 gtacaagctc gatgcagctg accgatgcgg tggtgaaaaa gatcgaccga aagcatgcga
     5341 aggtcaccct gcagcatggc gacatccccg atgtgatgcc ggcgatgacc atggcttaca
     5401 ggctcaagga tgcccgcctg atggatggcc tccatgtcgg cgactctgtc cgctttgcgc
     5461 tggaaaagtc gggcgacgac tacatcgtga ctcaccttga agtcgcacgc taagccggaa
     5521 atgaaacaga tgccgcgttt tttcggcatc acttaccaat aggaatgacc atgaatcgtt
     5581 ttatcgcatg gtgtctggtc gggtgcctct tgagcgggtt tgcctgtgcc gccgaagcgc
     5641 cattgggtgc ggatgtcggc ggtttgttga gctatgcgcg cgaacacaat ccggagctgg
     5701 cggctgcgcg ctatgaggca gaggcggtgc agcaacgcac cgaatctgcg ggtgcgttgc
     5761 ccgatccggt gttgcgtacc gagctgatgg acatcaccaa tcagggtacg aaatctgcca
     5821 gcctgaatcc gtcgcaagtc ggcggtacgc gctatctgtt gatgcaaact gttccctggt
     5881 tcggcaagcg cggtttgcag cgcgatgtgg ccgaggcaca ggcggctcag gccgacgggc
     5941 aggctgcggc tacctggacg gaactggccg gccagatcaa gacggcctat gccaactatt
     6001 actacctgtc ccgcagcgaa caactggcgc aacagacact gaagctgctg gtcgcgctgg
     6061 aacatatcgc ccatgaacgc tatgccaacg gtctggcgac gcagcaggag gtgataaggg
     6121 cgcaggtgga gcagaccgca ttgcgcggcg agctgatcga gttgcaaaac ggtgttcatc
     6181 accagcacag tcggctgaat gcgctgttgt cgcgtcccgg catggcggtg ttggcggaac
     6241 cggcgcagct gcggcccatc cccgctcagg cacggctcga ttacgccgtt ctggcggaca
     6301 ggttgcgtgc gcgcaatcct cagctgctga tggccgatgc gcgcagcaac gaggccgtga
     6361 agagccggga cctgacttac gtcaaccgct atcccggatt cacgctgggc gtcgcaccta
     6421 cccagagcgg caaccgggtg aagagctggg acctgatggt cgagatgaat attccgttgc
     6481 aacagggatc gcgtcgcgct caggagcgcg aggccgatgc gctgctttcc gccgccgaat
     6541 cgcgcaagca ggcgctgcag gacaggatgc tgaacatgct ttcggaaggc ctgtctggac
     6601 tggaatccgc acggcgcacc gaggcgctga caacaacgag tctgctgccg caggccgagc
     6661 tgacctatca gtctgcgctg gccggttacg agaacggcag ggtgggattt gccatgttgc
     6721 tggaggcgca gcagcaaatc ctgaaggcga agcagcaacg gcttaaggcc cagctcgatg
     6781 cgcaattgcg tctggccgaa atcgagaatc tgttgggaga agagctatga aaaatattta
     6841 tctgattttt atcgccgtgc tgctgggcgt cgcggcgggt tcctactggc tgggcttgaa
     6901 aggccggatc gaggatcgag gatcgagggt tgcggcgaaa agcgagcgac ctgttcttta
     6961 ctaccgcaac ccgatgggtt tgccggacac ttcgcccgtg ccgaagaagg atgcgatggg
     7021 catggattac gtgccggttt atgaaggcga atccgcagca gacggcgcga tcgacatcgg
     7081 cagcgacaag gtgcagaagc tgggcgtgaa gagcgaaccg gcggcgttgc gcgtgctgga
     7141 acacaagctg cgtgccgtcg gccgtatcga gatcgacgag cgccgcagct acactgtcgc
     7201 accgaaattc gaaggctggg tggagcgcct gtatgtgaac accaccggtc agatggtcgt
     7261 caaaggccag cccctgttcg aggtgtacag cccggaactg gtctcggcac aacgcgaaca
     7321 tgcgctggcg gtacaaggcg tggccgatat gaaggacgcc gacggcgagg cgcgggacag
     7381 catgaagcga ctggccgatg ccagtgccgc gcggctgcgc aactgggatg tcggcggtgc
     7441 ctccggcggc aagacggtga ctttccatgc gccggtctcc ggtatcgtgc tggagaagaa
     7501 ggccgtgcag ggtgtgcgtt tcatgccggg cgagatgctg tatcagatcg ccgatctgtc
     7561 ttcggtgtgg gtgatggccg acgtggccga gcaggatatc gcgtcgatca agctgggcga
     7621 tgcggtgcgg gtcggcgtcg atgcctatcc ggcgcagcgc ttcgacggca aggtcgattt
     7681 catctatccg acactgaaca gcgcgacacg cacggtgtcg ctgcgcgtcg tgatccccaa
     7741 ttcgcaaggc ctgctcaagc cttcgatgtt cgccagtgtg gaactggcga cgggcaagag
     7801 cgacaaggta ctcagcgtgc caatctcggc ggtgatcgac agcggcacca gacaggtggt
     7861 gctggtgcgt ctggctgaag ggcgttttgc accgcgcgag gtcaagaccg ggcgccgagg
     7921 cgatgcctat gtcgaagtgc tggcgggcat cgcggagggc gaacaggtgg tgacctccgc
     7981 caatttcctg atcgatgcgg agagcaatct cagggcggca ctgggcggtc tgggcggcgt
     8041 aacagctgct gccgaaccgt cctcgcagcc tgccgtgccg gataaaaaat ccgcatcggt
     8101 cgggcatcag gggcagggca agttcaacga gatcaatgaa gacggctcgg tcagcatcac
     8161 gcatgagccc atcgcatcgc tgggctggcc cggtatgacc atggacttcg cgctggccaa
     8221 ttccgcgctg acgaagggca tccagccggg cagcgcgatc aggttcgaga tcgtcgagcg
     8281 cgccaagggc gaatgggtga tcacccaatt gcaggcagcg ccgcagaaac atgaggggca
     8341 ttgagatgct gtccaaaatt atcgactggt cggcgcgcaa cacgtttctg gtgttgctcg
     8401 ccacgctgtt cgtcgtgctg gcgggcatct acgcgatcat cagaacgccg ctggacgcgc
     8461 tgcccgacct gtccgatgtg caggtgatcg tctacaccga atatgccgga caggcgccgc
     8521 aggtggtgga ggatcaggtg acctatccgc tgaccaccgc gatgctggcg gtgccgaaat
     8581 ccaaggtggt gcgcggtttc tcgttcttcg gcgcatcgtt cgtttacgtg atcttcgagg
     8641 acggtaccga catctactgg gcccgttcgc gcgtgctgga atatctcaac agcattgcgg
     8701 gccgcatgcc caagggcgtg tcgccgcaac tgggaccgga tgcgaccggg gtgggctggg
     8761 tttaccagta cgcggtgctc agcgagaagc acaatctcgc cgagctgcgc accatgcagg
     8821 actggtatct gcgttatcag ctgaccaagg cgcaaggcgt ggccgaggtc gcgtccatcg
     8881 gcggtttcgt gcagcagtat caggtgacgg tcgatccggt gaagctgcgc gcctacggca
     8941 tcccgctttc ccgcgtgtct caggtcatac gcgaatcgaa ccgcgatgtc ggcgggcgcg
     9001 tggtcgagat ggccgagacc gagtacatgg tgcgcggtcg tggctatctg catggcgtcg
     9061 gcgacatcga gagtctggtg gtgaaggccg agcggggcac gcctgtgctg ttgcgcgaca
     9121 tcgcgcgtgt cgagctcggg ccggatgaac ggcgcgggct gaccgacctc gacggcgagg
     9181 gcgaagtggt gtccggcatc gcgatggcgc gctacgggca gaatgcgctg gaagtgatag
     9241 cgaacatcaa ggccaagatc gccgagatca cgccgggctt gccggagggc gtttctattc
     9301 gtgcggtata cgaccgctcc gagctgatcc agcgcgcaat cgccacattg aagaagacgc
     9361 tgttcgagga aggcctgatc gtcgccgtcg tgtgcatggt gttcctgatg catgcaagga
     9421 gcgcgctggt cgccatcgtg atgctgccca taggcgtgct gatcgccttc atcgcgatgc
     9481 gcgcactcgg gttgaattcc aacatcatga gcttgggcgg catcgcgatc gccatcggcg
     9541 cgatggtgga tgcggccatc gtgatgatag agaacgcgca caagcatctg gagcggatgc
     9601 aggaaggcga gtcgcgtttc gatgcgatgt tgacggcctg caaagaggtg
//
LOCUS       DASUIV010000311         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_917299, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000311 DASUIV010000000
VERSION     DASUIV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917299"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2376
                     /locus_tag="VFW59_11130"
     CDS             <1..2376
                     /locus_tag="VFW59_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEX5364812.1"
                     /translation="EPSLNGIVNKALEGLKKLDPSPLASPARVSFIEAANYDQHLEQL
                     RGCDLIIEAIAERMDWKSDLYRKVAPYVSERAIFASNTSGLSINQLAQAFPEHLRHRF
                     CGIHFFNPPRYMHLVELIACSTTEAPLLDQLETFLVSTLGKGVVRAKDTPNFIANRIG
                     VFSMLATKHHAAAFNLGFDMVDALTGRYLGRPKSATFRTLDVVGLDVFAHVVNTMREN
                     LTDDPWHKHFELPGWFNYLVDQGSLGQKTKKGIYQKIGKEIHVLDLHTKAYRLSDAKV
                     DDGVKDILRERDWAKKLAGLRNSQHPQAQFLWAVFRDVFHYCALYLETIADNARDLDF
                     AVRWGFGWDSGPFEIWQAAGWQQVAEWIAADIAAGRAMANVPLPLWATDPTRTGVHTE
                     QGSYSPVTHFDQARSTLPVYRRQLFPDRLLGEERSYGETVFETDEVRMWHMNDGIAIL
                     SFKTKMHTIGNEVLDGMLRAVDEAEAHFSALILWQSEPPFSYGANLLQLMQGVQEPAE
                     DAGLFGKLKQAATRVKYTVAGGGGVGDLFNAATGNVPHVEAVVAKFQQVSQRLKYALV
                     PTIAAVDGLALGGGCEFSIHCSRIVATLESYIGLVEVGVGVLPAGGGCKEMAQRAAAE
                     AQRFANDNRIDVFPHLRRYFQNIAMGEVSKSAELAREMGYLKQSDRIVMNRFELLHVA
                     KEEARALNATAYRPPLHQRQIAVAGRTGIATFKAAMINMLEGGFISEHDYRIGCLVAE
                     TMCGGDVDAGSLVDEQWLLDLERKNFMELLATEKTQARVEHMLKNGKPLRN"
BASE COUNT          476 a          771 c          764 g          413 t
ORIGIN      
        1 gagccttcgt taaacggcat cgtcaacaag gcgctggaag ggctgaagaa actcgatccc
       61 agcccgctgg cgagcccggc acgcgtgagc ttcatcgagg cggctaacta cgaccagcat
      121 ctggaacagt tgcgcggctg cgacctgatc atcgaggcca tcgccgagcg catggactgg
      181 aaatccgacc tgtaccgcaa ggtcgcgcct tacgtttccg agcgcgcgat cttcgccagc
      241 aacacttccg gactctccat caaccagttg gcgcaagcct ttcccgagca tctgcgccac
      301 cgcttctgcg gcatccactt cttcaacccg ccgcgctaca tgcatctggt cgagctgatc
      361 gcatgcagca cgaccgaagc gccgctgctc gaccagctgg agaccttcct cgtctccacg
      421 ctgggcaagg gcgtggtgcg cgcgaaggac acgcccaact tcatcgccaa ccgcatcggc
      481 gtgttctcca tgctcgccac caagcatcat gcggcggcgt tcaatctcgg cttcgatatg
      541 gtggatgcgc tcaccgggcg ctacctcggc cgcccgaaga gcgcgacctt tcgcacgctg
      601 gacgtggtcg gcctcgacgt gttcgcgcat gtggtcaaca ccatgcgcga gaacctgacc
      661 gacgacccct ggcacaagca tttcgagttg ccgggctggt tcaactatct ggtggatcag
      721 ggctcgctgg ggcagaagac caagaagggt atctaccaga agataggcaa agaaattcac
      781 gtgctggacc tgcacaccaa agcatatcgg ctgtccgatg cgaaggtgga cgacggcgtg
      841 aaggacatcc tgcgcgagcg cgactgggcg aagaagctgg ccgggctgcg caacagccag
      901 catcctcagg ctcagttcct gtgggcggtg ttccgcgacg tgtttcatta ctgcgcgctg
      961 tatctggaga ccatcgccga caacgcgcgc gacctcgatt tcgcggtgcg ctggggcttc
     1021 ggctgggaca gcggcccgtt cgagatctgg caggccgcag gctggcaaca agtggccgag
     1081 tggatcgccg ccgacatcgc cgcaggccgc gcgatggcga acgtgccgct gcccttgtgg
     1141 gcgaccgacc cgacccgcac cggcgtgcac acagagcagg gctcctattc gcccgtcacc
     1201 catttcgatc aagcccgctc cacactcccg gtctaccgtc gccagctctt tcccgaccgt
     1261 ctgctgggtg aagagcggtc atacggcgag acggtgttcg agaccgacga ggtgcggatg
     1321 tggcacatga atgacggcat cgcgattctg agcttcaaga ccaagatgca caccatcggc
     1381 aacgaggtgc tcgacggcat gctgcgtgcg gtggacgagg ccgaggcgca tttctccgcg
     1441 ctgatcctgt ggcagagcga gccgccgttt tcctacggcg cgaacttgct gcaactgatg
     1501 cagggcgtgc aggaaccggc cgaagatgct ggcctgttcg gcaagctcaa acaggccgcg
     1561 acccgcgtca aatacaccgt tgcgggcggc ggcggagtcg gcgacctgtt caacgccgcg
     1621 accggcaacg tgccgcatgt cgaagccgtg gtcgccaaat tccagcaggt gtcgcaacgg
     1681 ctgaaatacg cgctggtgcc caccatcgcc gccgtggatg gcttggcact cggcggcggc
     1741 tgcgaattct ctatccactg ttcgaggata gtcgccacgc tggagagtta catcggtctg
     1801 gtcgaagtcg gcgtcggcgt gctgcccgca ggcggcggct gcaaggagat ggcgcaacgc
     1861 gcggcagcgg aggcgcagcg tttcgccaac gacaaccgca tcgacgtgtt cccgcatctg
     1921 cgtcgctact tccagaacat cgcgatgggc gaagtctcca agagcgccga actcgcgcgc
     1981 gagatgggct acctgaagca gtccgaccgc atcgtgatga accgtttcga actgctgcat
     2041 gtcgccaagg aagaggcccg cgcgctgaat gccaccgcct accgcccgcc gctgcaccag
     2101 cgccagatcg ccgtcgcggg gcgcaccggc atcgccacct tcaaggccgc gatgatcaac
     2161 atgctcgaag gcggcttcat ctccgagcac gactatcgaa tcggctgcct cgtcgccgaa
     2221 acgatgtgcg gcggcgatgt cgatgcgggc agcctggtcg acgagcaatg gctgctcgac
     2281 ctggaacgta aaaacttcat ggaactgctg gcgaccgaaa agacccaggc cagggtggaa
     2341 cacatgctga agaacgggaa accgctgagg aactagtgag tgggaagtcg ggttggcggg
     2401 gtgcgaaggt atgcggccta tata
//
LOCUS       DASUIV010000312         5388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_362426, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000312 DASUIV010000000
VERSION     DASUIV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362426"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1224
                     /locus_tag="VFW59_11135"
     CDS             244..1224
                     /locus_tag="VFW59_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364813.1"
                     /translation="MNLMFWKKKSPRDEEAGDTAPGLFARMKARVAALTGRFRTPPPF
                     QAEVDDDGNASRNDSADAGTAMPETGEKSGSRTKLIVGGTILLLLLLAAFGFATWKIF
                     MSSPDHDTDPLKSAAEQHPIAPSRPIVIPASAPSSLSASAAASAAPETATLSAPAETQ
                     VSADAQLEALRQKKAELQGMLDDRKLEHPPAPAETEHAGKASGAGDRVAASAEPKVTA
                     HEKSAASSVSQTRPLATAEPPAPSAQTPEAEIAKLRREKAELQRKLNEMRNEQRRTAA
                     TSRLHSGQAPVLGGSVTVPSNDPKATVDTLKTAIDAMNAGTGDYQKKPAK"
     gene            1255..2358
                     /locus_tag="VFW59_11140"
     CDS             1255..2358
                     /locus_tag="VFW59_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5364814.1"
                     /translation="MSSIQALTHPEILDQEALHEFADALTDHAPHIERNMARLSREPS
                     NRVVVADIFRALHNIKGDASLCRVETAVLIAHPIESLLTRLRSGEINYSPLLGEVVLL
                     ALDRLELAIEALLSGRTIEHLHLVALVDGLEKMSLAAAPDLDSVGIQLIKSVTGFQPQ
                     TLLRTLSESASIPSKPAEGMGADLHFFQTLALQFEARSALFSGRTERLLQLALDTNRA
                     AGAPVDTNQLKAAVYLHDVGMMFLPEATWLKTGKMSDAERNVLHTHPALAAELLDRME
                     GWKGAATMVRQHHEMWDGAGYPEGLSGDAICDGAKILAIVDAFEAVTLKHSSRGHKRS
                     ALRAIAEVNACDQQFSPEWIEPFNSVIRNMLEQ"
     gene            2461..3144
                     /locus_tag="VFW59_11145"
     CDS             2461..3144
                     /locus_tag="VFW59_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5364815.1"
                     /translation="MQNKETAPDISAFLASAIHDMKNSVSVLISFMNDNLTQLEPEAQ
                     RVAAPMFYEVQRVNDNLLQMLAIYKVGNDFYPFDLTENSIAEFMDEISAQSQSLLDYL
                     GIELDIEYDEDLRWYFDYELVSGTISHALHNASHYTNGKLKISARDEDGELVFRVEDN
                     GRGYPASVLEAGATVQKGVNFSSGSTGLGLYFSSMVAKLHKNRDKTGSIQLENGGTYG
                     GGCFSLRLP"
     gene            3157..4794
                     /locus_tag="VFW59_11150"
     CDS             3157..4794
                     /locus_tag="VFW59_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5364816.1"
                     /translation="MYKEVKDTTPLFASKSYLIIDDFQGMRTMMREMLRTFGGKNIDT
                     ATNAAEAIPLMEKNRYDVVLCDYNLGSGKNGLQILEEVKHRNLIGLATAWIIITAEKT
                     SDLVFGAAEHMPDDYIIKPVNESTMKGRLEKVLAKKEVLVNVEKAVRAKHHPLTVALC
                     DKLLASTQTNTVELLRIKTSTLMTMGQHDQAEQVFNQILAKRDIPWAKTGLGKVAFLK
                     GDMERAQQLFNEVIRDNPAYLEAHDWLVKTHESMGNLDEAQNVLARSVELSPTSVLRQ
                     RNLGEMAQKRGDLETAANAFRRTIKLGEHSIHKAPSTYLNLARVCSDKGTPAEALQVL
                     KNVTQEFEGAEIRLHAKVMEGSVYNKSGDHINSQKMSKELAAITSSHNTNLPPELMLE
                     ATQLFLATGNKEAAGNMAKSLVKNNHENAELLTQVSELYNKAGMHDIGDELVNKSREE
                     VIESNNRAVVMAKEGKLEEAIQLLREARVLLPNNKRIMINLANVSVLSMRQNGMNDEL
                     FKIAKDCMMRVAAIDPKEKWPAQIKAALDALAPQGNV"
     gene            4835..>5388
                     /locus_tag="VFW59_11155"
     CDS             4835..>5388
                     /locus_tag="VFW59_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364817.1"
                     /translation="MERIICVIGNKGGTGKTSITHMLCHGLGLIGKRSIAVLTDSMRE
                     PLERAGRRYTALDGRSPEKLDKIIEKLDSLPDWIGVIDGGGNRPEMDQQLYEMSGLVL
                     LPFRDSHEDIRTVRNDLDTFPRAYGLPSQWPTNPWQQLAAERTLDDLMQEYRPRLLDP
                     IYTVSSSKLLLETQIPPELPTALN"
BASE COUNT         1324 a         1697 c         1477 g          890 t
ORIGIN      
        1 ggacgaccgc gccggcgata gtcacatcac catcggcgat caactcagcg caccacttgc
       61 gggtgccaaa accctgcgac tgcaggatgc gatccagact cattttactc atggcggcac
      121 tttagcagaa gccgcacctc gttgcccgca tcgacgcatc ggcttgcgtg cgtttgacgc
      181 tggagggatg gcgcaatatg ctgcgctctg gcggttgagc atgccgcatg caatggagtc
      241 acgatgaacc tgatgttctg gaagaagaaa tccccacggg atgaagaggc gggcgacacc
      301 gcgcccggct tattcgcacg gatgaaagcg cgcgtcgccg cactgaccgg acgcttcaga
      361 acaccgccgc catttcaggc tgaagtggac gatgacggga acgcatcccg caacgattcc
      421 gccgatgcgg gcacggcgat gccggagacc ggcgaaaaat ccggctcacg caccaagctg
      481 atcgtcggcg gaaccattct gctgctactc ctgctggccg ccttcggttt cgccacctgg
      541 aaaattttca tgtcctcacc cgaccacgac accgatccgc tcaagagcgc cgcagagcag
      601 catcccatcg caccatcccg gccgattgtg atacccgcca gcgcaccatc gtcactctcc
      661 gcgtcggcgg cggcatctgc tgcaccggaa acggcaaccc tgtcggcccc tgctgaaacg
      721 caggtatcgg ctgatgccca gctcgaagcg ctcagacaga aaaaagccga gttgcagggc
      781 atgctcgacg accgaaaact ggaacatccc cctgcgccgg ccgaaacgga gcatgccggc
      841 aaagcaagcg gtgcaggcga tagagtcgca gcatctgccg agcccaaggt aaccgcgcac
      901 gaaaaatctg cagcctcgtc cgtctcacag acccgccctc tggcaaccgc cgagccgcca
      961 gccccatccg ctcaaactcc cgaggctgaa atcgcaaaac tcaggcggga aaaggccgag
     1021 ctccagagaa agctcaacga aatgagaaat gagcagcggc gcacggccgc caccagcagg
     1081 ctgcatagcg gtcaggcacc cgttctcggc ggctccgtga ccgtgccaag caacgacccc
     1141 aaagcgaccg tcgacacact aaaaaccgct atcgacgcca tgaacgcagg caccggcgac
     1201 tatcaaaaga aacccgccaa atgaccccag accaaccgac aaaggcacaa cgacatgagc
     1261 agcattcagg cattaactca tcccgagata ctggatcagg aagcactgca tgaatttgcc
     1321 gatgccctga ccgaccatgc gccccatatc gagcgcaaca tggcccggct gagccgggag
     1381 ccctctaacc gtgtagtggt cgcggatatc ttccgcgcct tgcacaacat caaaggcgat
     1441 gcctcgttat gccgggtgga aacggccgtc ctgatcgcac accccatcga atcgttgctg
     1501 acccgcctgc gctccggcga aataaactac tcgccgctgc tgggcgaagt ggtattgctc
     1561 gcactggaca gactggaact ggctatcgag gcgctgctgt cgggacgcac catcgagcac
     1621 ctgcatctgg tcgcgctggt tgacgggctg gaaaagatga gcctggccgc agcacctgac
     1681 ctcgactccg tcgggataca gttgatcaag tcagtgaccg gattccagcc gcagaccctg
     1741 ttgcgcacac tgagcgaatc cgcgtcgata ccgagcaagc ctgcggaagg aatgggcgcg
     1801 gatctgcatt tcttccagac cctcgccctg caattcgaag cgcgttctgc gctgttctcc
     1861 ggacgcaccg aacgcctttt gcaattggcc ctggacacca accgggctgc cggcgcccct
     1921 gtcgatacca accaactgaa ggcggcggtc tacctgcatg acgtgggcat gatgttcctg
     1981 cccgaagcga cctggctcaa gaccggaaaa atgagcgacg ccgagcgcaa cgtgttgcac
     2041 acccaccccg cactggccgc cgaattactt gatcgcatgg agggatggaa gggcgcggcc
     2101 accatggtgc ggcagcatca tgagatgtgg gatggtgccg gctacccgga agggctgagc
     2161 ggcgatgcca tctgcgacgg cgcgaagatt ctggccatcg tcgatgcatt cgaggccgtc
     2221 acgctcaagc acagcagccg cggccacaag cgctccgcgt tgcgagccat cgccgaagtc
     2281 aacgcttgcg accagcaatt ttccccggaa tggatcgagc cgttcaacag cgtgatccgc
     2341 aacatgctcg aacaatagcg caggcaaccc ggctcactgg cagaaggcct gcattcgccg
     2401 tacaatccgc gccatatcct gataccagcc ttcacggacc tgaagataat gcacaactta
     2461 atgcaaaaca aagagactgc gcccgacatt tctgcatttc tcgcctcggc gattcatgac
     2521 atgaagaatt cggtcagcgt cctgatcagc ttcatgaacg acaacctcac ccagctcgaa
     2581 cccgaagcgc aacgtgtggc ggcgccgatg ttttatgagg tgcagcgcgt caacgacaac
     2641 ctgctgcaga tgctggcgat ctacaaagtg ggcaacgact tctacccctt cgatctgacg
     2701 gagaactcca tcgcggagtt catggacgaa atctccgccc agagtcaaag cctgctggat
     2761 tatctcggca tcgaactcga tatcgaatac gacgaagacc tgcgctggta tttcgactac
     2821 gagctggtct ccggcacaat ttcacatgcg ctgcacaacg cctcccatta caccaatggc
     2881 aaactgaaaa tctcagccag ggacgaggat ggcgaactgg tatttcgtgt cgaagacaat
     2941 ggccgcggct accctgccag cgtgctcgaa gccggcgcca ccgtccagaa gggggtgaat
     3001 ttctcctctg gcagcaccgg actcgggcta tatttttcat cgatggtagc caaactccac
     3061 aagaatcgag acaaaaccgg ttccatacaa ctggaaaatg gcggcaccta tggcggcggc
     3121 tgtttcagcc tgcgcctccc ataagcgaga aactccatgt acaaggaagt caaagacacc
     3181 accccgcttt tcgcgagcaa aagctacctg atcatcgacg attttcaggg catgcgcacc
     3241 atgatgcgcg agatgctcag gacattcggc ggaaaaaaca tcgataccgc gaccaacgcg
     3301 gctgaagcga tcccattgat ggagaagaac cgctatgacg tcgtgttgtg cgactacaac
     3361 ctgggctcag gcaagaacgg cctgcagatc ctcgaggaag taaaacatcg caacctgatc
     3421 ggtctggcga ccgcatggat catcatcacc gccgagaaaa cttcggatct ggttttcggc
     3481 gcggccgaac atatgccgga cgactacatc atcaagcccg tcaacgaatc cacgatgaaa
     3541 ggtcgcctgg agaaggtact ggcaaagaaa gaggtgctcg tcaacgtgga gaaagctgtg
     3601 cgggccaagc accacccgct gaccgtcgca ctctgcgata aattgctggc ctcgacccag
     3661 accaataccg tcgaactgtt gcgcatcaaa accagcacgc tgatgaccat ggggcagcac
     3721 gatcaggccg aacaggtatt caatcagata ctggcaaaac gcgacatccc ctgggccaag
     3781 accggcctgg gcaaagtggc cttcctcaag ggagacatgg agcgagcaca acagctgttc
     3841 aacgaagtca tccgcgacaa tccggcctat ctggaagccc acgactggct ggtcaagact
     3901 cacgagagca tgggcaatct ggacgaggcg cagaacgtgc tggcacgcag cgtcgaactc
     3961 tccccgacct cggtcctgcg tcaacgcaac cttggcgaaa tggcgcaaaa acgcggcgat
     4021 ctggaaactg ccgccaatgc cttccgcagg accatcaagc tgggggagca ctctatccac
     4081 aaggcgcctt ccacttacct taacctggcc cgagtctgct cggacaaggg cactccggcc
     4141 gaggcgctgc aggtgctgaa gaacgttact caggaattcg aaggcgccga aatccgactg
     4201 catgcgaagg tcatggaagg ctcggtttat aacaaatccg gcgatcatat caactcgcag
     4261 aagatgtcca aggaactggc cgccatcacc agctcacata acaccaatct tccgcccgaa
     4321 ctcatgctcg aggcgaccca gctgttcctc gccacgggca acaaggaggc cgccggcaac
     4381 atggccaagt cgctggtcaa gaataaccat gaaaatgccg agctgctgac ccaggtcagc
     4441 gagctgtaca acaaagcggg catgcacgac attggcgacg agctggtcaa caaatcgcgc
     4501 gaggaagtca tcgaatccaa caaccgcgcg gtggtgatgg ccaaggaggg caagctggaa
     4561 gaagcgatcc agttgctccg tgaggcgcgc gtcctgctgc ccaacaacaa gcgcatcatg
     4621 atcaatctgg ccaacgtatc cgtgctgtcg atgcgccaga atggcatgaa cgacgaactg
     4681 ttcaaaattg ccaaagactg catgatgcgc gtcgccgcaa tcgacccgaa ggaaaaatgg
     4741 ccagcccaga tcaaggcggc gctagacgcc ctcgcccctc aaggcaacgt ataaaagcaa
     4801 cttagagccc caggaatcaa ccacggagca gctcatggaa aggatcatct gcgtcatcgg
     4861 caacaaaggc gggaccggca agaccagcat cacccacatg ctgtgccatg gtttgggctt
     4921 gatcggaaaa cgctctattg ccgtactcac cgatagcatg agagaacctc tggaacgggc
     4981 ggggcggcgc tataccgcgc tcgacggacg ctcgccggag aaactcgaca agatcatcga
     5041 gaaactcgat tcactgccag actggatcgg cgtgatcgat ggcggcggca atcgtccgga
     5101 gatggatcaa cagctctacg agatgtcggg cctcgtgctg ctgcccttcc gcgattcgca
     5161 cgaagatatc cgcacggtgc gcaacgatct cgacaccttc cccagagcct acggcctgcc
     5221 ctcgcagtgg ccgaccaacc cctggcaaca gctggccgcc gagcgcacgc tcgacgacct
     5281 gatgcaggag tatcgcccac ggctgctcga cccgatctac accgtcagct ccagcaagct
     5341 gctgctggaa acgcagatcc cccccgagct gcctacggca ctgaacaa
//
LOCUS       DASUIV010000313         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_362474, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000313 DASUIV010000000
VERSION     DASUIV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362474"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFW59_11160"
     CDS             complement(<1..182)
                     /locus_tag="VFW59_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5364818.1"
                     /translation="MISREDGRLVVRGRLTMETVPALFQAGVQHLASEDLLVDLSQVE
                     AVDSSAVSMLLGWSRAA"
     gene            complement(179..811)
                     /locus_tag="VFW59_11165"
     CDS             complement(179..811)
                     /locus_tag="VFW59_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5364819.1"
                     /translation="MKSLFAAVIGLLCVVGVARAEVVAPDALIAVTVQDVLSIVKQDK
                     DIQAGNQKKILALVDAKVLPHFDFTRMTQLAAGKHWRTATAEQKKALVAEFRNMLVRT
                     YAKAFSEYRNQTIDVLPFKMAADATDVTVKTEIKKPGGQPIAVDYEMRKSADGWKAFD
                     IVIGGVSMVMSYRGTFDSEVQQNGIDGLIKTLSDKNVTAANVALRKAEPK"
     gene            complement(823..1287)
                     /gene="mlaD"
                     /locus_tag="VFW59_11170"
     CDS             complement(823..1287)
                     /gene="mlaD"
                     /locus_tag="VFW59_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX5364820.1"
                     /translation="MERTTLDLWVGMFVVAGIAALVMLAMKVGNLSTYNVSETYEVHA
                     YFSNIGGLKPKASIKSAGVLVGRVTAITLDTERYEAKVIMNLDKRYQFPKDTFANILT
                     SGLLGEQYIGLVPGGDEEMLKSGDQVKMTQSAVVLEDLISKFLYSKAEEPAK"
     gene            complement(1291..2079)
                     /gene="mlaE"
                     /locus_tag="VFW59_11175"
     CDS             complement(1291..2079)
                     /gene="mlaE"
                     /locus_tag="VFW59_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEX5364821.1"
                     /translation="MPVAKTIRGIGRGAIDAVWRIGRAARFFFLILLYSGGSFRRFHL
                     TIKELFSTGVMSLIIIIVAGLFVGMVLGLQGYETLKRYGSESALGVMVALGLVRELGP
                     VVAALLFASRAGSAMTAEIGLMKATEQISAMEMMAVNPIARIVAPRFWAGVISMPLLA
                     ALFSAVGVYGGYFVGVVQIGVDEGSFWSQMQAAVDFREDILNGVLKSFVFGTAVTVIA
                     LFEGYDAPPTAEGVSGATTRTVVTSSLVILMLDFILTAMMFGGN"
     gene            complement(2090..2824)
                     /locus_tag="VFW59_11180"
     CDS             complement(2090..2824)
                     /locus_tag="VFW59_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5364822.1"
                     /translation="MQGINMTFPKGKLIAIMGLSGCGKTTLLRHIGGALKPTSGTIKI
                     DGQVLHDLDQDGLYAMRRKMGMLFQFGALFTDMSVYDNVAFQMREHTDLPEELIHDLV
                     LMKLHAVGLRGTHHLMPSELSGGMARRVALARTVALDPMLIMYDEPFAGLDPISLTVV
                     GDLIRRLNDALGATSIVVTHDIQESLKIVDYVYFMANGVIEAEGTPDEIRASGREFVH
                     QFVHGEMDGPVPFHYPGGDFRQDLNL"
BASE COUNT          674 a          960 c          648 g          544 t
ORIGIN      
        1 gccgcacgcg accagcccag caacatgctg acagccgatg agtcgaccgc ctcgacctgc
       61 gaaagatcca ccagcaaatc ttcgctggcc aaatgctgca cgccagcctg gaacaatgcg
      121 ggcaccgttt ccatagtcag acgaccacgc accaccagcc gcccgtcctc gcgtgaaatc
      181 actttggctc cgccttgcgc aacgccacat tcgcggcagt gacattcttg tccgacagtg
      241 tcttgatcaa accatcgata ccattctgct gaacttcgga atcaaaggtg ccccgataac
      301 tcatcaccat gctgaccccg ccgatcacga tatcgaatgc tttccagcca tcggcactct
      361 ttctcatctc ataatcgaca gcgatcggct ggccacccgg cttcttgatc tccgttttca
      421 cggtcacatc ggtcgcatca gccgccatct tgaaaggcaa aacatctatc gtctgattac
      481 gatactcgga gaatgccttg gcataggtgc gcaccagcat gttgcggaac tcggccacca
      541 gcgccttttt ctgttccgcc gtcgcggtgc gccaatgttt gcctgccgcc aattgggtca
      601 tgcgcgtgaa atcgaagtgc ggcagaacct tggcatccac cagcgccagt atcttcttct
      661 ggtttccagc ctgaatatct ttatcctgct tcacgatcga caggacatcc tgcacggtga
      721 ctgcgatgag cgcatccggt gcgaccacct cagcccgcgc cacgccaacc acacacaaca
      781 agccgatcac tgccgcaaac aaactcttca tatcaaacgc tcctatttag ccggctcttc
      841 agccttgctg tacaaaaact tgctgatcaa atcttccaac acaaccgccg actgcgtcat
      901 tttcacctga tcgccgctct tcagcatctc ctcatcgccg ccgggaacca ggccgatgta
      961 ctgctcgccc agcaaccccg aagtcagaat attggcgaac gtatccttgg gaaactgata
     1021 tcgcttgtcc agattcatta tcaccttggc ctcgtagcgc tcggtatcca gcgtaatggc
     1081 ggtcacccgc ccgaccagca cacccgcact cttgatcgag gccttgggct tcagaccgcc
     1141 gatattggaa aaatacgcgt gcacttcgta ggtctcggac acgttatagg tgctcaggtt
     1201 ccccactttc atcgccagca taaccagagc ggcgatacct gccaccacaa acatgccaac
     1261 ccacagatca agcgtcgtgc gttccatcgt tcaatttcct ccgaacatca ttgcagtcaa
     1321 aataaaatcc agcatcagga tcaccagcga cgaagtgacc acggtacgcg tagtcgcgcc
     1381 tgaaacaccc tctgcggtag gcggtgcgtc atacccctca aacagcgcga tcacggtcac
     1441 cgccgtaccg aacacaaaac ttttgagcac cccgttcagg atgtcctcgc gaaaatccac
     1501 ggcagcctgc atctgcgacc agaacgaccc ctcgtccacg ccgatctgca ccacgccgac
     1561 gaaataaccg ccgtacaccc ccacggcgct gaacagcgcc gccagcaacg gcatcgaaat
     1621 cacgccggcc cagaaacgcg gcgccacgat gcgcgcaatc ggattcaccg ccatcatctc
     1681 catcgcggag atctgctcgg tcgccttcat caagccgatt tctgcggtca tcgccgaacc
     1741 ggcgcggctc gcaaacaaca gcgcggccac caccggcccc agctcacgca ccagaccgag
     1801 tgcaaccatc acccccagag ccgattccga accgtaacgt ttaagcgtct cgtaaccctg
     1861 cagccccagc accatgccga caaacagccc cgccacaatg atgatgatca gcgacatcac
     1921 ccccgtggaa aacaattcct tgatggtcag atggaagcgc cggaagctgc cccccgaata
     1981 cagcagaata agaaagaaga agcgtgccgc acggccgatg cgccagaccg catcgatagc
     2041 gccgcgcccg attcctctga tggttttagc tacaggcata gtgtcaggct cacaaattca
     2101 aatcttgacg gaaatctccg cccggataat ggaatggcac cggaccatcc atctcgccat
     2161 gcacaaattg atgcacaaat tcccggccgg aagcgcggat ctcatcgggc gtaccctccg
     2221 cctcgatcac gccattggcc atgaagtaaa cataatccac gatcttcagc gactcttgta
     2281 tgtcgtgcgt caccacgatc gaagtcgctc ccagcgcatc gttcaggcgg cgaatcaagt
     2341 cccccaccac cgtcaacgag atcggatcca gtccggcgaa gggctcatcg tacatgatca
     2401 gcatcggatc gagcgccacc gtgcgcgcca gcgccacacg gcgcgccatg ccgccggaca
     2461 attcggacgg catgaggtga tgcgttccgc gcaaaccgac tgcatgcagc ttcatcaaca
     2521 ccagatcgtg aatcagctcc tccggcagat cggtatgttc acgcatctgg aacgccacat
     2581 tgtcatacac cgacatatcg gtaaacagtg cgccgaactg aaacaacata cccatcttgc
     2641 ggcgcatggc atacaagcca tcctgatcca gatcatgcag cacctgaccg tcgattttaa
     2701 tggttccgct ggtaggcttg agcgcgccgc cgatatgtcg caacaacgtc gttttgccac
     2761 agccgctcaa tcccatgatg gcgatcaact tccccttcgg aaaggtcatg ttgataccct
     2821 gcagga
//
LOCUS       DASUIV010000314         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_362795, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000314 DASUIV010000000
VERSION     DASUIV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362795"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1093
                     /locus_tag="VFW59_11185"
     CDS             14..1093
                     /locus_tag="VFW59_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5364823.1"
                     /translation="MQKKLIALAVAAAFSAPAFADNSNVTVYGKLNLDMENVQNDKAP
                     VRSVTRVSSNASRFGVKGSEDLGDGLSAIWQLEAQYNTANNTTTTVAGGAAGQTVTNG
                     AFDGVRNSNVGLKGNFGTVFLGNWDTPYKTTHNKVELFDNAGSFTSTLLIGHGGAAGG
                     TNFVTRQASDIQYWTPNLSGFTGKVSFASDTAQNAAAANTAKKNRLSMSGEYENDMLY
                     AALGYENRPAQTIASVTDTATRLVGAYKIDGSQIGLTYERLSIGTAANTSGTQNNWEL
                     SGNYKFGNSNLGAFYAQNGNIGATANSGAKMATLRYGYSFSKRTEVYGAYTSLKNDTA
                     ASYSIAGATAGSKQSAFGLGMIHNF"
     gene            1223..1975
                     /locus_tag="VFW59_11190"
     CDS             1223..1975
                     /locus_tag="VFW59_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX5364824.1"
                     /translation="MNDQQLQRYGRHILLNEIGIEGQQRLLDAQALIIGLGGLGSPAA
                     LYLASSGVGRLTLCDHDTVDFSNLQRQIIHRTSTVGQPKVVSARAVLNDINPEVECIA
                     LPLRVDETQLRELVAQADVVLDCSDNFATRYAINRACMAERKPLVSGAAIQFDGQVAV
                     FDHRRPEAACYNCLFPEDSQAAELRCATTGVFAPLVGMIGSLQAAEALKLLMGIERGL
                     SGKLLTLDALDMRIQRCNLSRDPACRVCSQTD"
     gene            1986..2495
                     /gene="moaC"
                     /locus_tag="VFW59_11195"
     CDS             1986..2495
                     /gene="moaC"
                     /locus_tag="VFW59_11195"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5364825.1"
                     /translation="MNELNHFSEAGRARMVDVAQKASTPRVAIASGILRMQPATLQRI
                     RDGQVSKGDVLTVADVGAVMAAKRTPDLIPMCHSIALTGVEVEFSEIIEPDAEGCVGI
                     RVFATVGCVGQTGVEMEALCAANVALLTIYDMCKAIDRGMRIECVQLEEKRGGKSGIW
                     LRADQAGAK"
     gene            2492..2731
                     /locus_tag="VFW59_11200"
     CDS             2492..2731
                     /locus_tag="VFW59_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5364826.1"
                     /translation="MINVLLFGPVAELAGTAMLQCEHRADLSLQDLRAELAAKYPQAF
                     EIVCFTAVNGEHARDFGQQLADGSEVVFMSKFSGG"
     gene            2736..3173
                     /locus_tag="VFW59_11205"
     CDS             2736..3173
                     /locus_tag="VFW59_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5364827.1"
                     /translation="MSAEIKEAVRIQLEDFSQDEELRALQASSKRMGGIATFLGCARD
                     FSEERTVTQLSFEAYGSMALAEMNRLRDEAIARFGLLDARIVHRIGTIAAGGQIVFIA
                     AGAEHRAPALEACHWLIDELKQRVPIWKKEVTPQGDAWVTPHP"
     gene            3170..3667
                     /gene="mobB"
                     /locus_tag="VFW59_11210"
     CDS             3170..3667
                     /gene="mobB"
                     /locus_tag="VFW59_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX5364828.1"
                     /translation="MNPPVFIFVGHSGCGKTTLIERLLPELSRRGLRVATIKHAHHKV
                     VLDTPGKDSWRYKQAGAVMSMLVTANELQLVADAVEQREPAQLAQRFLGEADMVLAEG
                     FSHASGVKIEVLRRECGKPPRCAIEDGLIAIVTDIDEVYPQLSHFALDDDARIVDFLL
                     EYAGL"
     gene            3664..>3886
                     /locus_tag="VFW59_11215"
     CDS             3664..>3886
                     /locus_tag="VFW59_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5364829.1"
                     /translation="MIADCTAVILAGGDSRRMGQDKAMLSLDGKPLLQHVMGAVTQVF
                     PYVMISVRQPRDGLALPQIRDARQHAGPLA"
BASE COUNT          808 a         1091 c         1207 g          780 t
ORIGIN      
        1 taaggagcat taaatgcaaa agaaactgat cgcgctggct gtcgctgccg cattctccgc
       61 accagccttc gctgacaaca gcaacgtaac cgtctacggc aagctgaacc tcgacatgga
      121 aaatgtccag aacgacaagg cacctgttcg cagcgttact cgtgtttcat ccaacgcctc
      181 ccgtttcggt gtgaagggca gcgaagatct gggtgacggc ctgtccgcta tctggcaact
      241 ggaagctcag tacaacaccg ctaacaatac cactactact gttgctggtg gcgcagccgg
      301 tcaaaccgtg actaacggcg cattcgacgg cgtgcgcaac agcaatgtcg gcctgaaggg
      361 taacttcggt accgtgttcc tgggcaactg ggatacccca tacaagacta cccacaacaa
      421 ggttgagttg ttcgacaacg ccggttcgtt tacttcgacg ctgttgatcg gtcacggcgg
      481 cgccgctggc ggtactaact ttgttacccg ccaagccagc gacatccagt actggactcc
      541 aaacctgagc ggcttcaccg gcaaggtttc cttcgcttcg gatacagcac agaatgcagc
      601 agctgcaaat actgccaaga aaaaccgtct gtcgatgtcc ggcgaatacg aaaacgacat
      661 gctgtatgca gcattgggtt atgagaaccg cccagctcag actatcgctt ctgtgaccga
      721 taccgctacc cgtctggtcg gcgcatacaa gatcgatggc agccaaattg gcctgactta
      781 tgagcgtctg tcgatcggta ctgccgccaa tacctctggc actcagaaca actgggagct
      841 gtcgggcaat tacaagttcg gcaacagcaa cttgggcgcg ttctacgcac agaacggcaa
      901 tatcggtgca acggctaatt ccggtgccaa gatggctacg ctgcgttacg gctacagctt
      961 ctccaagcgt accgaagtgt acggtgcata cacctcactc aagaacgata ccgctgcaag
     1021 ctacagcatc gcaggtgcta cagcgggttc caagcagtcc gctttcggtc tgggcatgat
     1081 ccacaacttc taatccggat cggctaacac cgattcaatt aggagaagca gtaaacgaga
     1141 cgggcacctt cgggtgcccg tttttatttg tgcccattcg ggctattctt cgcgcaggac
     1201 tcatacgcta ccctacaccc ttatgaacga ccaacaacta cagcgctacg gccgccacat
     1261 cctgctcaac gagatcggta tcgagggaca gcagaggctg ctggatgcgc aggcgctgat
     1321 catcgggctg ggcggtctag gctcgcctgc cgcgctgtat ctggcctcca gcggcgtcgg
     1381 cagattgacg ctgtgcgatc acgataccgt cgatttcagc aatctgcagc gccagatcat
     1441 tcatcgcacc tcgaccgtgg gtcagcccaa ggtcgtatcg gcacgggccg tgctgaacga
     1501 catcaacccg gaggtcgagt gcatcgccct gccgctccga gtcgatgaga cgcaattgcg
     1561 ggagctggtc gcacaggccg atgtggtgct ggattgcagc gacaatttcg ccacccgcta
     1621 tgcgatcaac cgtgcctgca tggcagaacg caagccgctg gtatcggggg cggcgatcca
     1681 gtttgatggc caagtggcag tgttcgatca tcgtcgtccg gaggctgcct gctacaattg
     1741 cctgttcccc gaggacagcc aggctgcgga gttgcgctgc gcgaccacgg gcgtgttcgc
     1801 gccgctggtc ggcatgatcg gcagcttgca ggcggcagaa gcgctcaagc tgctgatggg
     1861 gatcgaacgc ggtctcagcg ggaagctgtt gacgctagat gcgctggata tgcgcattca
     1921 gcgctgcaac ctgagccgcg acccggcttg ccgtgtttgt agccagaccg attgaaggat
     1981 caaaaatgaa cgagttgaac catttttccg aggcgggacg ggcgcgtatg gtggacgtgg
     2041 ctcagaaagc cagcaccccg cgcgtcgcca ttgccagcgg catcctgcgg atgcagcccg
     2101 caacgctgca gcgcatccgt gacggacaag tgagcaaggg tgatgtgttg acggtggccg
     2161 atgtcggcgc ggtgatggcg gcgaaacgca cacccgatct cattccgatg tgccactcca
     2221 ttgcactgac gggcgtggag gtcgagtttt ccgagatcat cgagccggat gcggagggct
     2281 gcgtcggtat cagggtgttc gccacggtcg gctgtgtcgg tcagaccggc gtggagatgg
     2341 aagcgttgtg tgcggcgaat gtcgcgctgc tgaccatcta cgatatgtgc aaggccatcg
     2401 atcgcggcat gcgcatcgaa tgcgtgcaac tggaagagaa gcgcggcggc aagagcggta
     2461 tctggttgcg ggccgatcag gcaggtgcga aatgatcaat gtattgctgt tcgggccggt
     2521 agcggaactt gccggtacgg ccatgttgca gtgcgagcac agggcggatc taagcctgca
     2581 agatctgcgc gccgaactgg cggctaaata tccgcaggca ttcgagatcg tctgctttac
     2641 cgcagtgaac ggcgaacatg cccgagactt cgggcagcaa ctggccgatg gcagcgaggt
     2701 ggtgttcatg tcgaagtttt ccgggggctg aaacgatgag cgctgaaatc aaagaagcgg
     2761 tacgcatcca gctggaagac ttttctcagg acgaggagct tcgtgcgtta caggccagct
     2821 cgaagcgtat gggtggcatc gccacttttt tgggctgcgc gcgagatttc tcggaagagc
     2881 gtaccgttac ccagttgagc ttcgaggctt acggcagtat ggcgctggcg gaaatgaaca
     2941 ggctgcgcga cgaggccatc gcccgcttcg gcctgctgga tgcgcgcatc gtgcatcgca
     3001 tcgggacgat tgctgcggga ggccagatcg tgttcatcgc ggcgggtgcc gagcatcgcg
     3061 ccccggcgct ggaagcctgc cattggctga tcgacgagct caagcagcgc gtgccgatct
     3121 ggaaaaaaga agtcacgcca cagggggatg cctgggtaac cccgcaccca tgaatccgcc
     3181 cgtctttatc tttgtcggtc actccggttg cggcaagacc acgctgatcg agcggctgtt
     3241 gcccgagttg agtcgacggg ggttgcgcgt tgccaccatc aaacatgccc atcacaaggt
     3301 ggtgctggac acgccgggca aagacagctg gcgctacaag caggccggtg cggtgatgag
     3361 catgctggtg actgccaacg agttgcagct ggtcgccgat gcggtcgagc aacgcgaacc
     3421 ggcgcagctg gcacagcgct ttctgggtga ggccgatatg gtgctggcag agggtttttc
     3481 ccatgcgtcc ggcgtgaaga tcgaggtatt gcggcgcgaa tgcggcaagc cgccgcgttg
     3541 cgccatcgaa gatggattga ttgccatcgt gaccgatatc gacgaggtct atccgcaact
     3601 gtcgcatttt gcgctggacg atgacgcccg catcgtcgat ttcctgctgg aatacgcggg
     3661 gctatgattg cggattgcac ggccgtcatt ctggctgggg gtgattcccg tcgtatgggg
     3721 caggacaagg cgatgctgtc tctggacgga aagcccctgc ttcagcacgt gatgggtgcc
     3781 gtaacacagg tatttccgta tgtgatgatc agcgtacgcc aaccccgtga tggattggcg
     3841 ttgccgcaga tccgtgatgc gcggcaacat gccgggccgt tagccg
//
LOCUS       DASUIV010000315         4657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1199780, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000315 DASUIV010000000
VERSION     DASUIV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199780"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFW59_11220"
     CDS             <1..240
                     /locus_tag="VFW59_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5364830.1"
                     /translation="DEDDKAIVKAIILMARSLDFTTIAEGVETAEQLAFLRECGCDEV
                     QGYYFSKPLPAESLITFMKQSSLPSAAVAAGFIAG"
     gene            complement(237..932)
                     /locus_tag="VFW59_11225"
     CDS             complement(237..932)
                     /locus_tag="VFW59_11225"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5364831.1"
                     /translation="MAFMNPHDVADNDRHAVARRLMVEEIRAMANETGDVVARPISDR
                     VLSVMAEVPRHRFVPEGESYCAYFNHPLPIGYGQTISQPYIVALMTDLLALDRRHRVL
                     EVGTGSGYQTAVLSEMAGKVYSMEIVEPLAARAANLLRKLGYANVQVKSGDGHAGWPE
                     QAPFDSIIVTAAADRIPQPLLDQIKPGGRMVIPVGDCSYVQELILITKDIDGLSHRKD
                     ILPVRFVPLTGAD"
     gene            complement(974..1135)
                     /locus_tag="VFW59_11230"
     CDS             complement(974..1135)
                     /locus_tag="VFW59_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364832.1"
                     /translation="MLSKPHIRRLLAALLMALGAAMMFLAPETWAGILLLVLGASVEL
                     IGIVLKRKA"
     gene            complement(1140..2270)
                     /gene="dnaJ"
                     /locus_tag="VFW59_11235"
     CDS             complement(1140..2270)
                     /gene="dnaJ"
                     /locus_tag="VFW59_11235"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5364833.1"
                     /translation="MITAQRDYYEVLGVAKDAEPKAIKDAFRNLALKYHPDRNKEAGA
                     EERFKEIAEAYAILSDPKKRAEYDSRGFAGVEGFSQEDLFSGINFDDIFGGLNFDFGS
                     SPFEGFFHRRRHAGPVRGDNIEVELFIPLERVASGGEEKVRLGRPVTCRACHGTGAEG
                     GAAPKACETCKGSGRITQSRRQEKEHLLIQQISTCPACHGRGSIVEHPCTKCHGSGQE
                     DMEETLTVKIPRGVEEGMALRISGKGMPSPESGGVSGDLFVVVRSKHDPRFERAGADL
                     LRQENIPLTDAVLGATLNVPTLDGSASVEIPPGTQPGSVLRLKGKGLPEFGSARHGEL
                     YLRIEVQVPETLTQEERQLYERLRTLGGKTPKTSASHWWQRS"
     gene            complement(2312..>4657)
                     /locus_tag="VFW59_11240"
     CDS             complement(2312..>4657)
                     /locus_tag="VFW59_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364834.1"
                     /translation="GEVIGQMRAMEAIRFGTGIRHEGYNLFVLGPPGTGKHGIVRQFL
                     EQKVAGEREPTDWCYVNNFAQPHKPHALKLPSGKGPELHQRMEKLVDYLRSAIPALFE
                     GEEYHAKAEAIHQEFSKQHEQLFKDLGEDAEKQEIALLRTPDGFAFAPTRSDEAHNHE
                     VIPPDEYEKLPDEKKKQIEATIAGLQERLEKIMRQIPQWRRERHEQIKQLDRETALSA
                     IGQLVAELKEAYTDLPEVRSYLDQVQQDMVDNVDDFRKQEETATFGGMTMVAHHSFHR
                     YQVNVLVTNGKKPGAPVVTEDNPTYSNLIGRVEHIAQLGALITDFTLIKPGALHRANG
                     GYLLLDIRKVLMQPFAWEGLKRAMQAHEIRIESLGQMYSLVSTVSLEPEPIPLDAKVI
                     LFGDRLFYYLLQLYDPEFVELFKVAADFEDRIERNADTHLLYARLIATLARREKLLPL
                     DCGAVARVIEHSARLVGDAERLSMHMSGVVDLLREADYCAREAGRNAVAKTDVQCAID
                     AQIRRQDRIRERLYEAILRDTLVIDTEGTMTGQVNGLAVIELGNFSFAQPTRITATTR
                     LGEGELVNIEREVKLSGAIHSKGVLILTSFLSARYARNQPLALSASLVFEQSYGQVEG
                     DSASLAELCALLSNLADAPIKQSLAITGSVNQFGQAQAIGAVNEKIEGFFDICNARGL
                     SGKQGVLIPEANVKHLMLRHDVVAAAAAGKFHIYAVESVDQAIALLTGLSAGKADAKG
                     DYPEGSVNRRVAARLSELIEIRASFARRLKNKTATKNKTEQ"
BASE COUNT          778 a         1459 c         1425 g          995 t
ORIGIN      
        1 gacgaggacg acaaggccat cgtcaaagcc atcattctga tggcgcgcag cctggatttt
       61 acgacgatcg cagaaggcgt cgaaaccgcc gagcaactgg cgttcctgcg cgaatgcggt
      121 tgcgatgagg tgcagggtta ctatttcagc aagccgttgc cagccgaatc gctaatcacc
      181 ttcatgaagc aatcgtcgct cccctctgcg gcggtcgcag ccggattcat cgcgggttaa
      241 tccgcacccg tgaggggtac gaaacgtacc ggcagaatat ctttccggtg ggataggcca
      301 tcgatatcct tggtgatcag aatcagctcc tgaacatagg agcagtcccc caccggaatc
      361 accatgcgtc cgcccggttt gatctgatcc agcagcggct ggggaatccg gtccgcagct
      421 gcggtgacga taatgctgtc gaatggcgcc tgttcgggcc agcctgcgtg accatcgcct
      481 gactttacct gcacgttggc atagcccagt ttcctcagca gattcgcggc ccgtgcggcc
      541 agcggttcga cgatctccat gctgtatacc ttgccggcca tctcggacag aaccgcagtc
      601 tgatagccgg agccggtgcc gacctccagc acgcgatgcc gcctgtccag cgccagcagg
      661 tcggtcatca gcgccacgat gtagggctgc gagatggtct gtccgtagcc gatgggcagc
      721 gggtggttga aataggcgca gtagctttca ccctctggga cgaagcggtg acgtggaacc
      781 tcggccatca ccgagagcac cctgtccgag atcggcctcg cgacaacgtc gccggtctcg
      841 ttcgccattg cgcggatttc ctcgaccatc agacgccgcg ccacggcatg acgatcattg
      901 tcggccacat catgtggatt catgaatgcc atgatgactc cggaagtcct ggaatgtgcg
      961 cggacatggg gggctacgcc ttgcgcttga ggacaatgcc gatgagttcg acagacgcgc
     1021 ccagcaccag cagcaatatt ccggcccagg tttcgggcgc cagaaacatc atggccgcac
     1081 ccagcgccat cagcaaggcc gccaagagac gacggatatg aggtttgctc agcatgatgt
     1141 cagctccttt gccaccaatg gctggcagaa gttttaggcg ttttgccgcc cagggtgcgc
     1201 agccgctcat agagctggcg ttcttcctgt gtaagcgtct ccggcacctg cacctcgatg
     1261 cgcaggtaca gttcgccgtg acgggcactg ccgaattccg gtagtccttt gcccttgaga
     1321 cgcagcaccg agcccggctg ggtgccgggc ggtatttcga cgctggccga tccgtccagc
     1381 gtcggcacat tcagcgtggc gccgagcacg gcgtcggtca gggggatgtt ctcctggcgc
     1441 agcaggtcgg caccggcccg ctcgaagcgc gggtcgtgct tcgagcgcac caccacgaac
     1501 aggtctccgc tcactccgcc ggactccgga ctcggcatgc ccttgccgga gatgcgcaga
     1561 gccatgcctt cctcgacgcc gcgcggtatc ttcacggtca gggtctcttc catatcctcc
     1621 tgcccgctgc cgtggcactt ggtgcagggg tgttcgacga tgctgccgcg cccgtggcat
     1681 gccgggcagg tgctgatctg ctggatcagc aggtgttcct tttcctggcg gcggctttgc
     1741 gtgatgcgcc cgctgccctt gcaggtctcg caggccttgg gtgcagcccc gccctcggcg
     1801 ccggtgccgt ggcaggcgcg gcaggtgacg gggcggccca gacggacttt ttcctcaccg
     1861 ccgctggcca cccgttccag cgggatgaat agctccactt cgatattgtc gccgcgcacc
     1921 ggaccggcgt ggcggcggcg atggaagaag ccttcgaacg ggctgctgcc gaaatcgaaa
     1981 ttcagcccgc cgaagatgtc gtcgaagttg atgccgctga acaggtcttc ctgactgaag
     2041 ccctcgacac cggcgaatcc gcgcgagtcg tattcggcgc gcttcttcgg atcggacagg
     2101 atcgcatagg cctcggcgat ttccttgaag cgttcttcgg caccggcttc cttgttgcgg
     2161 tcgggatggt atttcagggc gaggttgcgg aaggcatcct tgatcgcctt cggttcggca
     2221 tccttggcaa cgccgagcac ctcgtaatag tcacgctggg cagtaatgat tgttcccccg
     2281 ggttgcggag gggcacgggc gcgccgtgtc cctactgttc ggttttattc ttcgttgctg
     2341 tcttgttctt caaccgccgg gcgaacgacg ccctgatttc gatcagttcg ctcaggcgtg
     2401 ctgcgacgcg gcggttgacg ctgccttccg gatagtcgcc ctttgcatcg gccttgcctg
     2461 ccgacaggcc tgtcagcagc gcgatcgcct gatcgacact ttccaccgcg tagatgtgga
     2521 attttccggc ggcggctgcc gcgacgacat catggcgcag catcaggtgc ttgacgttag
     2581 cctcgggaat cagcacgccc tgcttgccgg agaggccgcg cgcattgcag atgtcgaaga
     2641 agccctcgat cttctcgttg accgcgccga tcgcctgcgc ctggccgaac tggttcaccg
     2701 agcctgtgat cgccagcgac tgtttgatcg gcgcatcggc cagattggaa agcagcgcgc
     2761 acagctcggc cagcgaggcg ctatcgccct cgacctgtcc gtaggattgc tcgaacacca
     2821 ggctggcgga aagcgccagc ggctggtttc tggcatagcg tgcagagagg aaggaggtga
     2881 ggatcagcac gcccttggaa tggatcgcgc cagagagttt gacttcgcgc tcgatgttga
     2941 ccagctcgcc ttcgcccagc ctcgtggtgg cggtgatccg ggtcggctgg gcgaacgaaa
     3001 aattgcccag ctcgatcacg gccaggccgt tgacctgacc tgtcattgta ccttccgtgt
     3061 ctatgaccag cgtgtcgcgc aggatcgcct cgtacagccg ttccctgatc ctgtcctggc
     3121 gccggatctg ggcatcgatg gcgcactgca catcggtctt ggcgactgcg tttcgacccg
     3181 cttcgcgcgc gcagtaatcg gcctcgcgca gcaaatccac cacgccgctc atgtgcatcg
     3241 agagcctttc cgcatcgccg accagacgcg cgctgtgttc gatgactctc gccaccgcgc
     3301 cgcaatccag cggcaacagc ttctccctgc gcgccagcgt cgcaatcagc ctcgcataca
     3361 gcaggtgggt atcggcattg cgctcgatgc gatcctcgaa gtctgcggcg accttgaaca
     3421 gctcgacgaa ttccgggtcg tacagttgca gcaggtaata gaacaagcga tcgccgaaca
     3481 ggatcacctt ggcgtccagc ggaatcggtt cgggctccag cgacacggtg ctgaccaggc
     3541 tgtacatctg tcccagcgac tcgatgcgta tctcgtgcgc ctgcatcgcg cgcttcagac
     3601 cttcccatgc gaacggttgc atcaatacct tgcggatatc cagcagcaga tagccgccgt
     3661 ttgcgcggtg caacgcaccc ggcttgatca gcgtgaaatc ggtgatcaag gcgcctaact
     3721 gggcgatgtg ttcgacacgc ccgatcaggt tgctgtaggt cgggttgtct tcggtgacga
     3781 caggcgcgcc cggttttttt ccgttggtca ccagcacgtt cacctgatag cggtggaagc
     3841 tgtggtgggc gaccatcgtc atcccgccga aggtcgcggt ctcctcctgt ttgcggaagt
     3901 cgtcgacgtt gtcgaccatg tcctgctgta cctgatcgag gtagcttcgc acctcgggca
     3961 ggtcggtgta ggcctctttc agctcggcta ccagttggcc gatcgccgac agtgcggttt
     4021 cccggtcgag ttgtttgatc tgctcatgcc gttctctgcg ccattgcggt atctgccgca
     4081 tgatcttttc cagccgctcc tgcaacccgg ctatcgtcgc ctctatctgt ttctttttct
     4141 cgtccggcag cttctcgtat tcgtcgggcg gaatcacctc gtgattgtgg gcctcgtcgc
     4201 tgcgggtggg ggcgaaggcg aaaccgtccg gggtgcgcag cagggcgatc tcctgcttct
     4261 ccgcatcctc gcccaaatcc ttgaacaatt gttcgtgctg cttgctgaat tcctgatgaa
     4321 tggcttcggc cttggcgtga tattcctcgc cttcgaacag cgcggggatc gcgctacgca
     4381 aataatcgac cagcttctcc atccgctggt gcaactccgg acccttgccg gatggcagct
     4441 tgagtgcatg gggtttgtgc ggttgcgcga agttgttgac atagcaccag tcggtcggtt
     4501 cgcgttcgcc tgcgaccttt tgttcgagga actgccgcac gatgccgtgc ttgccggtgc
     4561 ccggcggacc gagtacgaac aggttatagc cttcgtggcg gatgccggtg ccgaagcgta
     4621 tcgcctccat cgcacgcatc tggccgatga cctcgcc
//
LOCUS       DASUIV010000316        11508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_640190, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000316 DASUIV010000000
VERSION     DASUIV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640190"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFW59_11245"
     CDS             complement(<1..192)
                     /locus_tag="VFW59_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364835.1"
                     /translation="MSELVGRALLATSMPLSAAARDSVGLQANYTYAESTGTTNSITG
                     LNQPLTNLSPHSYNLNGWLD"
     gene            complement(622..697)
                     /locus_tag="VFW59_11250"
     tRNA            complement(622..697)
                     /locus_tag="VFW59_11250"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(662..664),aa:Thr,seq:cgt)
     gene            complement(731..1789)
                     /gene="thiO"
                     /locus_tag="VFW59_11255"
     CDS             complement(731..1789)
                     /gene="thiO"
                     /locus_tag="VFW59_11255"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX5364836.1"
                     /translation="MHSDFLIIGGGAVGLTSAQALLQMGYRVTLVERGEIGQEASWAG
                     GGILSPLCPWDYGDAVTRLALRSMGMFGETAFMLQASTGIDPEYRRCGMLLLPPFQDQ
                     LASRWCQQHEVAMQHVDLAAQLDGLQGRGLLLPEIAQVRNPRLLHAMRERVKIFGGKI
                     IERCEVQEFEVAGDRVTGLSTAQGRLSADAYVVAAGAWSKRLLGGHALSLEVKPIRGQ
                     ILLFKFDEPPFQQMLLKGSLYFIPRQDGHVLVGSTLEDVGFDKSVTVEAKDSMLQRVR
                     EVFPAWPEPLRQWAGFRPGSPDNIPTIGRHPQLVNLYANTGHFRYGVTMSLASAELLL
                     NEIEGEAQPIPVDAYRWH"
     gene            complement(1797..2720)
                     /gene="ispH"
                     /locus_tag="VFW59_11260"
                     /gene_synonym="lytB"
     CDS             complement(1797..2720)
                     /gene="ispH"
                     /locus_tag="VFW59_11260"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5364837.1"
                     /translation="MQVLLANPRGFCAGVDRAITIVERALAIHGAPVYVRHEVVHNKF
                     VVEGLRQKGAVFVEELDDVPSGSILIFSAHGVPLSVRHEAEKRGLTVFDATCPLVTKV
                     HLQVSRLRAQGREIVMIGHAGHPEVEGTMGQSEGGMYLVESPEQVQQLQVSDEHNLAY
                     VTQTTLSMDDANDIVVALRARFPDIVGPKKDDICYATQNRQDAVKLLVKQCDVVVVVG
                     SENSSNSNRLREVAAKVGVPAYLVSDADGLKPEWFDGKLSVGVSAGASAPEVLVQGVL
                     ERLRQFGALQVNELEGIVENVVFPLPKSLQD"
     gene            complement(2811..3155)
                     /locus_tag="VFW59_11265"
     CDS             complement(2811..3155)
                     /locus_tag="VFW59_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364838.1"
                     /translation="MSIPGLHLTQIGGGDFLLDPTKSLNICDKDQLIESISAHLMQVR
                     ANRLYYDLSDLGLIDPLYYAWLDALARAARVMNIEMICIGMQPTAAFALARFLDGPPS
                     FRTALEIQDWQN"
     gene            complement(3169..4032)
                     /locus_tag="VFW59_11270"
     CDS             complement(3169..4032)
                     /locus_tag="VFW59_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5364839.1"
                     /translation="MVTKKALSEKVKALFALKVSGIASDLEAPGMRFFCGDSRLMDGE
                     EVADISHEFESLIASLLSAKDPSDMNSAEFSAMKAFFSNLSHQVLMRGGHVEDVVRYT
                     QTLQFSLIDALEKSSGVAFGQSRSVLLFFTGIFNELIMAVFGSYLEEREQTLQSQQAE
                     LRETATPITEIWDGVLTLPIIGTLDSNRTMLVMEALLNRISKDHAKAVVMDLTGVKSI
                     DSQVSHHLIQMVRAVQLMGSDAIVTGIHPEIARALVSLNIDFNNITTRANMADGLKEA
                     FRRMNITVSHV"
     gene            complement(4070..5110)
                     /locus_tag="VFW59_11275"
     CDS             complement(4070..5110)
                     /locus_tag="VFW59_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364840.1"
                     /translation="MNDIIDSAKDCKMLHSVQTKDESSLVVLRSKLFSATNRLGVSDL
                     KRENILLVASELATNNIKHAGGRGMIQIWQQPGPVLDILSLDYGPGINDLALAEQDGY
                     SSVNTFGKGLGAIRRLSDESYVYTQVKGGEQMTKWTGTVFLARFNIMSDKTRMLGGAV
                     GLFSRSLSDKRYNGDRIYLQKKGDKLRWLHLDGLGHGEEAQIATANLTAHVIHNDGLA
                     AMLDGVDRQLAGTRGAVAVAGELTVSKRDLHLLGVGDMHAHIFEEDEMNHVSFAPGIL
                     GREHRTMTPFHARLPKRAVVLTASDGIRRNWDIGNFVGLPNQHPQLMAYTLGNIMGRV
                     SDDQSLCVLFAG"
     gene            complement(5107..6054)
                     /locus_tag="VFW59_11280"
     CDS             complement(5107..6054)
                     /locus_tag="VFW59_11280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5364841.1"
                     /translation="MQNFIDFVLKSVGALGGNLFPASMALLELLERLRGEGAATGQPM
                     MVRLVLEDRQLFAQWGDAKNFRIVAFPHEPSVEQVAELSAFLANSVALADPEILLQRN
                     AEMARHFEEARAKAEHELAGLQSMLEQRQREMQEFDLQAHTDALTGLFNRRAFDERLK
                     SAFLHTMRQKASPLSLLLFDLDHFKEINDEFGHQFGDAYLNKMAVVLHEIIREDVDFA
                     FRFGGDEFAVMLFANHAVACEKANLVLRLMENKVSIGIATIDRHTPDSQKLEEFIHRA
                     DDALYAAKRGGRGRAIIATCASQASGKCCSPCPEMDCLR"
     gene            complement(6239..6547)
                     /locus_tag="VFW59_11285"
     CDS             complement(6239..6547)
                     /locus_tag="VFW59_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5364842.1"
                     /translation="MRVSRLLKDDTATVYIAGRFDATGYRQFRDVCDECLRLPEVVTL
                     DVDVSGVEHFNSTALGMLILVRAEARKWGKALRLSNPDELTWRVLELANFHRLFAIVR
                     "
     gene            complement(6826..7296)
                     /gene="lspA"
                     /locus_tag="VFW59_11290"
     CDS             complement(6826..7296)
                     /gene="lspA"
                     /locus_tag="VFW59_11290"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5364843.1"
                     /translation="MLKRWLGLSLLVVVLDQFSKLWISSLFVYGETLRVLDVFDLVLA
                     HNTGAAFSFLHDAGGMQRWLFSAIAVIASVWIAVLLRKHATQKLFALALSLILGGALG
                     NLIDRIAYGYVVDFLSFHWDTHYFPAFNLADSAITLGAALLILDSLRPVHRIAR"
     gene            complement(7289..10084)
                     /gene="ileS"
                     /locus_tag="VFW59_11295"
     CDS             complement(7289..10084)
                     /gene="ileS"
                     /locus_tag="VFW59_11295"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5364844.1"
                     /translation="MTDDKKKYPLNLPDTAFPMRGDLAKREPQMLARWQTQQRYRQIR
                     AAKKGRPQFILHDGPPYANGDIHIGHAVNKILKDIIVKSKTLSGFDAPYVPGWDCHGL
                     PIELQVEKKHGKAIPPSQFRELCREYAKEQVERQKKDFIRLGVLGDWDRPYLTMDFGT
                     EADIIRALGKIAERGYLYQGKKPVNWCLDCQSALAEAEVEYEDKVSPAIDVGFEVEDK
                     GALDRAFGVSLPGEARVYAVIWTTTPWTLPANQAVSVHPEHEYSLVKTDKGYLLLIAE
                     LMESCLQRYELSGETVGQCKGSALENLPLLHPFQDRIVPVICGEHVTLEAGTGLVHTA
                     PAHGVDDYVIGQKYNLPNENPVGDDGKFISTTPAVGDTPLAGVFVWKANDIVLAAMEA
                     SGHLLHQEKLKHSYPHCWRHKTPIIFRSTPQWFIGMEQGDEGKLRDLANQAVAATQFF
                     PSWGRARLEAMIRNRPDWCVSRQRNWGVPMPFFVHKETMQLHPRTPELLEQVAKLVEQ
                     SGIEAWFSLNSVDLLGADAAHYRKLSDTLDVWFDSGATHYAVLARREGLRAPADLYLE
                     GSDQHRGWFQSSLLTGCAINGRAPYDALLTHGFVVDGKGHKMSKSKGNVIAPQKIFDT
                     MGADILRLWVAMTDYSGELTISDEILKRVVESYRRIRNTLRFLLANIADFDTAQHALP
                     VQEWLAIDRYALALMRNLQDEIVADYGRYEFHLVAQKLQHFCSEQLGGFYLDILKDRL
                     YTAGEHSHARRSAQNALHHLAHALVRLMAPVLSFTGEEAWETLSGKQDASVFIDEWHA
                     LPDALMDGEEFGDWLKIEAWRARVNKQLEEARALGLIGSALAAEVDVFVAGEDYDVLA
                     RLGNDLRLVFITSRATVHRVATEADQHIDVAASLHEKCERCWHYREDTGSDAAHPTLC
                     GRCVSNLFGDGEVRKYA"
     gene            complement(10077..11018)
                     /locus_tag="VFW59_11300"
     CDS             complement(10077..11018)
                     /locus_tag="VFW59_11300"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX5364845.1"
                     /translation="MQILRGLSLAGLRTAPSEHVALTVGNFDGIHLGHQALLDELRIE
                     AGQRGLKTAVVIFEPHPREFFTPQQAPARLTSLREKLELFRAMGVDRVHVCRFDAQLA
                     QMDAADFIHALHEKLNVRFVLIGDDFRFGSGRAGDFAMMERIGSQGGFEVRAVHSVLH
                     DGVRISSTGIRAALAAGQLRRAHDYLGRPYSISGRVMHGDKLGQKIGFPTANIQLKHN
                     LPPLAGIYVVQVHAEGLGILQGVASLGVRPTVKQNAKPVLEVHLFEFAQQIYGKHLRV
                     EFLQKLRDEEKYPDLESLTKQIALDVAHAKKWFEQHD"
     gene            11080..11475
                     /locus_tag="VFW59_11305"
     CDS             11080..11475
                     /locus_tag="VFW59_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HEX5364846.1"
                     /translation="MLDYPQVALPFMNHDHAEFIELRERLLAALAKQPDITEVDALLA
                     RLLEHTRNHFGEEERLMQEAQFPPYPMHKGEHDRVLADMKTRVAQWQQDRDAEGLRGW
                     MDKWVGEWFVNHVSTMDFMTARFIAATQG"
BASE COUNT         2523 a         3651 c         3083 g         2251 t
ORIGIN      
        1 atccagccag ccgttgaggt tgtaactatg cggcgacaga ttggtcagcg gctggttcag
       61 cccggtgatg ctattggtcg tgccggtgct ctcggcataa gtgtagttcg cctgcaagcc
      121 gacgctatcc cgcgcagcag cggaaagcgg catactagtt gcgagcaacg cgcgccccac
      181 cagttcagac atccgcctcc cgaagcgatc gcccctattg ctcagttcag tcatatcact
      241 taccagctcg actttcctca tacattgacg gaaacaaata ccgttcgctc agccctggaa
      301 gaatataatc aagacagagc ttgcaacgcc agcactacag aacttccgaa gaaataagct
      361 accaacctga acaggtaagg ataccaataa cacatatttt aaattgtagt cttatggtgt
      421 tcgttttggc tagatgcacc gagttcaaat aatcaggaaa cagaataatg aagattcggg
      481 accgatttgg gaatgcgttg cgggcaggca cgcggattga cgcaggaagg cttttcgtcg
      541 ttatcaagcc aaacctattt gagcacagtc gagtgcggcc gacactctga aaaaactgaa
      601 gcgttcagga aaagaaaaac atggtgccgg cgatgtgaat cgaacacacg acctacgcgt
      661 tacgagtgcg ttgctctacc aactgagcta caccggcatg aaccttgcga attataggag
      721 aggtggcggc ttagtgccag cggtatgcgt ccaccgggat cggctgcgcc tcgccttcaa
      781 tctcgttgag cagcaactcg gcactggcca gcgacatggt gacgccgtag cggaaatggc
      841 cggtgttggc gtaaagattg acgagctgcg gatgacgtcc gatggtgggg atattgtccg
      901 gcgaaccggg gcggaatcca gcccactgcc tgagcggttc cggccatgcc gggaaaactt
      961 cacgcactcg ctgcagcatg gagtccttcg cttcgacagt caccgacttg tcgaagccaa
     1021 cgtcttccag cgtgctgccg accagcacat gaccatcctg acgaggaatg aaatacaaac
     1081 tccccttgag caacatctgc tggaacggcg gttcatcgaa cttgaacaac aatatctgtc
     1141 cgcgtatcgg tttaacctcc agactcagcg cgtgtccgcc cagcagtctc ttgctccacg
     1201 cgcctgccgc aacgacatag gcatcggcag agagcctgcc ctgagccgtg ctcagccccg
     1261 tcacccgatc gcctgcgacc tcgaactcct gcacttcgca tcgctcgatg atcttcccgc
     1321 cgaaaatttt cacccgttcg cgcatcgcat gcaacagacg cggattgcgt acctgcgcga
     1381 tctccggcag caacagccct ctgccctgca gaccgtccag ctgagccgcc agatcgacat
     1441 gctgcatggc aacttcgtgc tgctggcacc accgggaggc aagctgatcc tgaaaaggcg
     1501 gcagcagcaa catgccgcat cggcgatact cgggatcgat gccggtagag gcctgcaaca
     1561 tgaaagccgt ctcaccgaac atgcccatgc tgcgcaaagc cagccgcgtg accgcatcgc
     1621 cgtaatccca cgggcacagc ggcgacagaa tgccgccgcc agcccatgag gcctcctgcc
     1681 cgatctcgcc gcgctccacc agcgtcactc gatagcccat ctgcagcagc gcctgtgcgc
     1741 tggtcaagcc taccgcgccg ccgcctatga tcaggaagtc gctgtgcatg cccgcatcaa
     1801 tcctgcaaac tcttgggcaa agggaacacc acgttctcga cgatgccctc cagctcgttc
     1861 acctgcagcg cgccgaattg gcgcagccgc tcgagcaccc cctgcaccag cacctccggg
     1921 gcggaggcac ccgcgctgac gcccacgctg agcttgccgt cgaaccattc tggctttaac
     1981 ccgtcggcat cgctcaccag ataggcgggg accccgacct tcgcggcgac ctcgcgcaat
     2041 cgattggaat tggagctgtt ctccgaaccg accacgacca ccacatcgca ttgcttgacc
     2101 agcagcttga ccgcatcctg acgattttgc gtggcatagc agatgtcgtc tttcttgggg
     2161 ccgacgatat ccgggaaacg cgcccgcagc gcgacgacga tatcgttggc atcgtccatc
     2221 gacagggtag tctgggtcac ataagccaga ttgtgctcat cgctcacttg cagctgctgt
     2281 acctgttcgg gagactccac cagatacatg ccgccctccg actgccccat ggtgccttcc
     2341 acttccggat ggccggcatg accgatcatg acgatctccc tgccctgcgc gcgcagccgc
     2401 gacacctgga gatgcacctt ggtgaccagc gggcaagtcg cgtcaaacac cgtcagaccg
     2461 cgcttctccg cctcatggcg caccgatagc ggcacgccat gcgcgctgaa gatcaggatg
     2521 ctgccgctcg gcacgtcgtc cagttcttcg acgaacaccg cccccttctg acgcagccct
     2581 tccacgacga atttgttatg caccacttcg tggcgcacat agaccggtgc accgtgtatg
     2641 gccagagccc gttcgacgat cgtgatggcg cgatccacgc cggcacagaa accgcgcgga
     2701 ttggcgagca atacttgcat gggatttatc cttgaagcgt tgatgaatac gccccggatt
     2761 ctaccgcagc ccgccccgct ctgcgcgcgc gattccggcg aaccgcaatc ctaattctgc
     2821 cagtcctgta tttccagagc cgtccggaaa gacggcggac catcgaggaa tctggccaac
     2881 gcgaaagccg ccgtcggctg catgcctatg cagatcattt cgatattcat tacccgagct
     2941 gcgcgggcga gtgcatccag ccaggcgtaa taaagcggat cgatcaatcc gagatcggaa
     3001 agatcgtaat acagacgatt cgcccgcacc tgcatgaggt gcgccgagat ggactctatc
     3061 aattgatcct tgtcacagat gttcaggctc ttggtcggat ccagcagaaa gtctccgccg
     3121 ccgatctggg tcaggtgcaa accgggaata gacatcttgc cccaatgctc aaacgtgact
     3181 gacggtgatg ttcatgcgcc ggaacgcctc tttcagtccg tcggccatat ttgcacgcgt
     3241 agtgatattg ttgaaatcga tgttcaggct gaccagcgcc cgcgcgatct ccggatggat
     3301 gccggtcacg atcgcatccg accccatcag ctgcaccgcc cgcaccatct ggatcaggtg
     3361 atgcgagacc tgggaatcga tgctcttcac cccggtcaaa tccatgacca ccgccttggc
     3421 atgatccttg gaaatacggt tgagcaacgc ttccatgacc agcatggtac ggtttgaatc
     3481 gagcgtaccg atgataggca gggtcagcac accgtcccat atctcggtga tcggcgtggc
     3541 ggtctccctg agttcggcct gctgcgactg caacgtctgc tcacgctcct caaggtagga
     3601 accgaacacc gccatgatca gttcattgaa aattccggtg aagaaaagca acaccgaccg
     3661 tgactggccg aaggcaacgc cgctggactt ttccagcgca tcgatcaggg aaaactgcaa
     3721 ggtctgggta tagcgcacca cgtcttccac gtgaccgccg cgcatcagca cctgatggga
     3781 caaattggaa aaaaacgcct tcatcgcaga gaactccgcc gagttcatgt cgctgggatc
     3841 tttcgccgat aacagcgagg cgatcaggct ctcgaattca tggctgatat cggcgacctc
     3901 ctcgccatcc atcagtcggc tgtccccaca gaaaaacctc attcctggcg cttccagatc
     3961 ggatgcgata ccgctcacct tgagcgcgaa cagcgccttg accttctcgc tcagagcctt
     4021 ctttgtcacc atgttcttct cccttatcta tcggttattt gattattgtc tatcctgcaa
     4081 ataaaacgca cagcgattgg tcatccgaca cccgccccat gatattgccc agcgtgtaag
     4141 ccatcaactg cggatgctga ttgggcaaac cgacgaagtt accgatatcc caattgcgcc
     4201 ggatgccgtc gctggccgtt aacaccaccg cccgcttggg cagcctggca tgaaacggcg
     4261 tcattgtcct gtgctcccgc cccagtattc ccggtgcaaa ggaaacatga ttcatttcat
     4321 cctcctcgaa aatatgggca tgcatatcgc ccacaccgag cagatgcaga tcgcgcttcg
     4381 agacagtcaa ctcgccagca accgccacag caccacgcgt ccccgccaac tgacgatcca
     4441 cgccatccag catcgcggcc agcccgtcgt tatggatgac atgcgccgtc agattggcag
     4501 tcgcgatttg cgcctcctcg ccatgcccca agccatcgag atgcagccag cgcaacttgt
     4561 cgcccttttt ctgcagatag atacgatcgc cgttgtagcg cttgtcggag agtgaacgcg
     4621 agaacaatcc caccgcaccg cccagcatgc gagtcttgtc gctcatgatg ttaaagcgcg
     4681 caagaaatac cgttccggtc cacttagtca tctgctcgcc acccttgacc tgagtgtaaa
     4741 cataggactc gtcactcaaa cggcgaatag cgcccaaccc cttgccgaag gtattgacgc
     4801 tggaatagcc atcctgttcg gccagcgcca gatcgttgat gccgggacca taatcgagcg
     4861 acaggatatc cagcacaggg ccgggttgtt gccatatctg gatcatgccg cgaccaccgg
     4921 catgcttgat gttgttggtc gccagttcgg aggcgaccag caggatattt tcccgtttca
     4981 ggtcggaaac gccgagacgg ttggtggcgg aaaacagctt ggagcgcaac accaccagcg
     5041 aactctcgtc cttggtctga acgctgtgca gcatcttgca gtccttggcg ctatcgataa
     5101 tgtcgttcat cgcaagcagt ccatttccgg acagggcgaa cagcattttc cgctcgcctg
     5161 agatgcgcag gtcgcgatga tggcccgacc acgtccaccg cgcttggccg catacaaggc
     5221 atcgtcggcg cggtggatga actcttccag cttctgacta tccggcgtgt gtctgtcgat
     5281 ggtagcgatg ccgatactca ccttgttttc catcaacctc agtaccagat tcgccttctc
     5341 gcaggcgacg gcatgattgg cgaacagcat cacggcaaac tcatcaccgc cgaagcggaa
     5401 ggcgaaatcc acatcctcgc ggatgatctc gtgcaaaacg acggccatct tgttcaggta
     5461 ggcatcgccg aactgatggc cgaattcgtc gttgatttcc ttgaagtgat cgagatcgaa
     5521 aagcagcaga gacaacgggg aagccttctg gcgcatggtg tgcaggaagg cacttttcag
     5581 cctctcgtcg aaggctcggc gattgaataa gccggtgagt gcatcggtat gggcctgaag
     5641 atcgaattcc tgcatttccc gctgacgctg ctccagcata gattgcaacc cggccagctc
     5701 atgctcggct ttagcgcgag cctcctcgaa gtggcgcgcc atctcggcat tgcgctgcaa
     5761 gagtatctcc gggtccgcca atgccaccga gttggccaga aatgcactca actcagcgac
     5821 ctgttcaaca gaaggttcat gaggaaaagc gacgatcctg aaattctttg catcgcccca
     5881 ctgggcaaat agctgacgat cctcaaggac caagcgaacc atcatgggct gaccggtcgc
     5941 agctccctcg ccacgcaacc gttcaagcaa ctcgagcaag gccatggaag caggaaacag
     6001 gttaccgccg agcgcaccca cgcttttcag cacaaaatcg atgaaatttt gcacatccgg
     6061 ttcgactgca aggttttgct tcagaatttc ctctgccccc acctgctccc ccacctgtcc
     6121 atgtacccac gttgaacgtg cgtgatttga acataaaaac cccctgtcag gggaaactcg
     6181 aaggcctata tacttttcga cttaagtgaa aaaaaccacg cacagaaggt tctgaagctc
     6241 agcgcacgat ggcaaacaag cgatggaaat ttgccagctc cagcactctc catgtcagct
     6301 catccggatt gctcaaccgt agtgcttttc cccattttct ggcttctgca cgcaccagta
     6361 tcaacatacc caatgccgta ctgttgaagt gttccactcc tgaaacatcg acgtccagag
     6421 tgaccacctc cggcaaccgc aggcactcat cgcatacatc cctgaattga cggtaaccgg
     6481 tggcatcgaa gcggcctgca atataaacag tcgcggtatc gtcctttaac aatctgctaa
     6541 cacgcataaa actccttgtt actcgccaac accactgccg cccagcctgg tttcgtcgct
     6601 aattcgagca tggctcaaca tttcaatggg atcccgtttc gatcatcgga tctccccgga
     6661 tagtgcgatg gagtgtatgg gcaatatcct ccaagggata taggcctaaa tatataggcc
     6721 taaatatata gattggaagg tatgacgcta aatcgtcagg gtgacgtgct tctgcaattc
     6781 aagcccaaaa gaaacaggac ctgcatctaa agcaggccag atccctcaac gagcgatacg
     6841 atggactgga cgcagactgt ccagaatcaa cagggcagcc cccagggtga ttgccgagtc
     6901 ggcaagattg aaggcaggga aataatgggt atcccagtgg aacgacagga agtcgaccac
     6961 ataaccgtag gcgatgcggt cgatcaggtt gcccagcgcc ccgccgagga tcaggctgag
     7021 cgccagtgca aacaacttct gcgtcgcatg tttgcgcaac aacacggcga tccacaccga
     7081 ggcaatcacg gcgatggcgc tgaacaacca gcgctgcatg ccacccgcat catgcagaaa
     7141 actgaatgcc gcgccggtat tgtgtgccag caccagatcg aagacgtcca ggacacggag
     7201 cgtctcgccg tagacaaaaa gactggatat ccacaacttg ctgaactgat cgagcacgac
     7261 caccagcaac gagagcccca gccaacgctt aagcatattt tctgacctcg ccatcaccga
     7321 acagattgct cacacagcgt ccgcacaaag tgggatgagc ggcatcgctt cccgtatcct
     7381 cacggtaatg ccagcatcgc tcgcattttt cgtgcaggct ggcggcgaca tcgatgtgct
     7441 gatcggcctc cgtcgcgacg cgatgcaccg tggcgcgcga ggtgatgaac accagacgca
     7501 gatcgttgcc cagacgcgcc agcacgtcat agtcctcgcc cgcgacaaac acatccacct
     7561 cggcggccag cgccgaaccg atcaatccga gggcgcgcgc ctcttccagc tgtttgttca
     7621 ctctggcacg ccaggcctcg atcttcagcc agtcgccaaa ttcttcgccg tccatcagcg
     7681 catcgggcag ggcatgccat tcgtcgatga acacgctggc atcctgttta ccggacagag
     7741 tctcccatgc ctcctcgccg gtaaagctga gtaccggagc catcaggcgc accagcgcat
     7801 gggccagatg gtgcagcgca ttctgcgccg aacggcgcgc atgggaatgc tcgcccgcag
     7861 tgtacaagcg gtctttcagg atatcgagat agaaaccgcc cagctgttcc gagcagaaat
     7921 gctgcagctt ctgcgccacc agatgaaact cgtaacgccc gtaatccgcg acgatctcgt
     7981 cctgcagatt gcgcatcagc gccagcgcat aacggtcgat cgccagccat tcttgtaccg
     8041 gcagcgcatg ttgcgcggtg tcgaaatcgg cgatgttggc cagcaggaaa cgcagcgtgt
     8101 tgcggatacg gcgatagctt tccaccacgc gcttgaggat ctcgtcggag atggtcagct
     8161 cgccggagta gtccgtcatc gccacccaca ggcgcaggat gtccgcgccc atggtgtcga
     8221 aaatcttctg cggcgcgatc acgttgccct tggacttgga catcttgtga cccttgccgt
     8281 ccaccacgaa accgtgcgtc agcagcgcgt catagggtgc gcgtccgttg atcgcgcagc
     8341 cggtcagcaa gctggactgg aaccagccgc gatgctggtc cgagccttcc agatacaggt
     8401 cggcaggcgc gcgcaacccc tcgcgtctgg ccagcaccgc atagtgcgtc gcaccggaat
     8461 cgaaccaaac atccagcgtg tcggacagct tgcggtaatg cgccgcgtcc gcgcccagca
     8521 gatcgaccga gttcaggctg aaccaggcct cgatgccgga ctgttcgacc agcttcgcca
     8581 cctgctccag caactccggc gtacgcgggt gcagctgcat ggtctccttg tgcacgaaga
     8641 acggcatcgg cacgccccag ttgcgctggc gcgacacgca ccagtcggga cgattcctga
     8701 tcatcgcctc gagacgcgcg cgcccccagg acgggaagaa ctgcgtcgcg gcgacggcct
     8761 gattggccag atcgcgcagc ttgccctcgt cgccctgctc catgccgatg aaccactgcg
     8821 gcgtggagcg gaagatgatc ggcgtcttgt gacgccagca atgcggatag ctgtgcttga
     8881 gtttttcctg atgcagcaga tggccgctgg cctccatcgc ggccagcacg atgtcgttgg
     8941 ccttccacac gaacacgccc gccaggggcg tatcgcccac ggccggggtg gtggagatga
     9001 acttgccgtc gtcccccacc ggattctcgt tcggcaggtt gtatttctgg ccgatcacat
     9061 agtcgtccac gccatgcgcc ggcgcggtat gaaccagacc ggtacccgcc tccagcgtga
     9121 catgttcgcc gcaaatcacg ggcacgatgc gatcctgaaa cggatgaagc agcggcagat
     9181 tttccagcgc cgaacccttg cactgtccga ccgtctcacc gctcagctcg tagcgctgca
     9241 gacaggattc catcagctcc gcgatcagca gcagatagcc cttgtcggtc ttcaccagac
     9301 tatattcgtg ctcgggatgc acgctcaccg cctgattggc cggcagcgtc cagggcgtgg
     9361 tcgtccagat caccgcgtac acacgggcct cgcccggcag agacacgccg aaggcacgat
     9421 ccaacgcgcc cttgtcctcc acctcgaagc ccacgtcgat cgcgggcgag accttgtcct
     9481 cgtactccac ctcggcctcg gccagcgcgg actggcaatc cagacaccag ttcaccggct
     9541 tcttgccctg atacagatag ccgcgctcgg cgatcttgcc cagcgcgcgg atgatgtcgg
     9601 cctcggtgcc gaaatccatg gtcagataag gtctgtccca atcgcccagc acgccgagac
     9661 ggatgaaatc tttcttctgg cgttcgacct gctccttggc atattcgcgg cacagctcgc
     9721 gaaactgcga gggcgggatc gccttgccgt gctttttctc tacctgcagt tcgatcggca
     9781 ggccgtgaca gtcccagccc ggcacatagg gcgcatcgaa gccggagagc gtcttgctct
     9841 tgacgatgat gtccttgagg atcttgttga ccgcatgacc gatgtggata tcgccgttgg
     9901 cgtaaggcgg gccgtcgtgc aggatgaact gcggccggcc cttcttggcc gcgcgtatct
     9961 gccgatagcg ctgctgcgtc tgccagcgtg ccagcatctg cggctcgcgt ttggccaggt
    10021 cgccgcgcat cggaaaggcg gtatcgggaa gattaagcgg atattttttc ttgtcgtcag
    10081 tcatgttgtt caaaccactt tttcgcatgc gccacatcga gcgcgatctg tttcgtcaaa
    10141 ctttccaggt ccggatactt ctcttcatcg cgcagcttct gcaaaaactc cacgcgcaga
    10201 tgcttgccat aaatctgctg ggcaaactcg aacaggtgta cttccagcac cggtttggca
    10261 ttctgcttca cggtcggccg cacgcccaga ctcgccaccc cctgcaagat gcccaaccct
    10321 tcggcatgca cctgcaccac atagataccg gccagcggcg ggagattatg cttcaactgg
    10381 atattcgcag tcggaaaacc gatcttctgc cccagcttgt cgccatgcat cacacgcccg
    10441 ctgatgctgt agggccgccc cagataatcg tgcgcccggc gcaactgccc cgccgccagc
    10501 gccgcgcgga tgccggtgct ggaaatacgc acgccgtcgt gcagcacgct gtgcaccgca
    10561 cgcacctcga agccgccctg cgaaccgatc cgttccatca tggcaaagtc accggcacgt
    10621 ccgctgccga aacggaagtc atcgccgatc agcacgaagc ggacattcag cttctcatgc
    10681 aaggcatgga taaaatccgc cgcatccatc tgcgccagct gcgcatcgaa acggcagaca
    10741 tgcacccgat ccacccccat cgcgcgaaac agctccagct tttcgcgcag actggtcagc
    10801 cgcgccggtg cctgctgcgg cgtgaagaac tcgcgcgggt gcggctcgaa gatcaccacc
    10861 gccgtcttca gcccgcgctg cccggcctcg atgcgcaatt catccagtaa cgcctgatgt
    10921 ccgaggtgga taccatcgaa attgccgacc gtgagcgcca cgtgctcaga cggagccgtg
    10981 cgcaagcctg caagagaaag tccgcgtaga atctgcatag ggcggcgatt atacagcgag
    11041 acgcaccccg aacacccatt cgcacaccaa ggaaaacaca tgctcgacta cccgcaggtc
    11101 gctctgccct tcatgaatca cgaccacgcc gagttcatcg aactgcgcga acggctgctc
    11161 gccgcactgg ccaaacaacc cgacatcacc gaagtggacg cgctactggc ccgcctgctg
    11221 gaacacaccc gcaaccattt cggcgaagaa gaacggctga tgcaggaagc tcagttcccg
    11281 ccctacccca tgcacaaagg cgaacacgac cgcgtgctgg ccgacatgaa aacccgcgtc
    11341 gcgcaatggc aacaggatcg ggatgcggaa ggcttgcgag gctggatgga taaatgggtc
    11401 ggcgaatggt tcgtcaatca cgtcagcacg atggacttca tgacggcgag gttcattgcg
    11461 gcaacgcagg ggtaatggtc ggcaggttgg gttgagcgca gcgatacc
//
LOCUS       DASUIV010000317         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_269261, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000317 DASUIV010000000
VERSION     DASUIV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269261"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2551
                     /locus_tag="VFW59_11310"
     CDS             <1..2551
                     /locus_tag="VFW59_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364847.1"
                     /translation="SYVPEKQILALQAKVLRSTALYSTGILLLLFVGCWYFTKMRYSQ
                     LKAQQDLGIAAAEYAKQLAIRDTEARMYAILHTIADGIITFSENGIIEEFAANAEYIF
                     GYSSEEVIGQNISMLMPESIHWLRDDYLMRAGKDASVAGGEHEITGRHKDGNSFPLEL
                     AVSEMMLGGQRHYTCMVRDITRRKRIQEELIAAKYDAELASQAKSYFLANMSHEIRTP
                     MNAIIGFTDLCLKTELAPMQRDYLEKVSLSANSLLGIINDILDFSKIESGKLDMEKVP
                     FNLDAVLKGVATVISIRAEEKRLEFLIDVAHGIPQMLVGDSLRLGQVLNNLANNAVKF
                     TETGEVAIQIRLERLTPGEGETYGQALLRFTVRDTGIGMTPEQIGKLFQSFSQADVST
                     TRKYGGTGLGLAISKRLVELMGGEIWVESTPGQGSRFTFSIPFTFLPDEFGAQPDLSK
                     LRVLVVDDNESARHLMLSYLESFGIEAVSAPDSLDGLAVIEHADASGQAFGCVVLDWS
                     MSGMSGLEMARRIKHELPIKQRPKIIYLSGHKHTEMINVSGAAKLLYAVINKPVTPSA
                     LFNALMACASGQRDTVQPLPENEAPPDLTGLHVLLVEDNKFNQQLANALLVRAGVKVG
                     IADDGLEALLALQREKFDVVLMDMQMPKMDGLEATRQIRKIPALAKLPIIAMTANAMA
                     GDRETCLAAGMNDYISKPLHYQAMYATIARWAHRGGSPTAPAEAPPTGEAPSILDTDR
                     ASAAMGGKALYLSMLAKFIPSQGQSVQSIRDALAADDRKTAERLAHSLKGIAATVGAA
                     ALAESAERLEQALRTEDSKAYPELLEIAASELRLAEASIEAYLEEHRKNAY"
BASE COUNT          600 a          794 c          801 g          457 t
ORIGIN      
        1 gtcctatgtc ccggaaaaac agattctggc attgcaagcc aaagtgctgc ggtctaccgc
       61 gctttactcg acgggaatac tgctgctgct gttcgtgggt tgctggtact tcaccaagat
      121 gcgctattcc cagctcaagg cccagcagga tctcggcatc gccgccgccg aatacgccaa
      181 gcaactggcg atacgcgata ccgaagcgcg catgtacgcg atactgcata ccatcgccga
      241 cggcatcatc accttcagcg agaacggcat catcgaagag tttgccgcca atgccgagta
      301 cattttcggc tattcctccg aggaagtcat cggacagaac atcagcatgc tgatgccgga
      361 gtccatccac tggctgcgcg acgactacct gatgcgggca ggcaaggatg cgagcgtcgc
      421 cggtggcgag cacgaaatca cgggacggca caaggacggc aacagttttc cgctggagct
      481 ggcggtcagc gagatgatgc tgggcggaca gcgccattac acctgcatgg tgcgcgacat
      541 cacgcggcgc aaacgcatcc aggaagaact gatagccgcg aagtacgacg cggaactcgc
      601 cagccaggcc aagagttact tcctcgccaa catgagccac gagatccgca cgccgatgaa
      661 cgcgatcatc ggcttcacgg acttgtgcct gaaaaccgag cttgcgccca tgcagcgcga
      721 ttatctggag aaggtttcgc tgtcggcgaa ttcgctgctg ggcatcatca acgacattct
      781 ggatttttcc aaaattgaat ccggcaaact ggatatggaa aaagtgccct tcaatttgga
      841 tgcggtgctg aaaggggtag ccaccgtcat cagcatccgc gcggaagaaa agcggctgga
      901 gtttctgatc gatgtggcgc atggcatccc gcagatgctg gtcggcgact cgttgcgcct
      961 gggccaggtg ctgaacaacc tcgccaacaa cgccgtgaag ttcaccgaaa ccggcgaggt
     1021 tgcgatccag atcaggctgg agaggctgac gcccggcgag ggcgagactt acggtcaggc
     1081 gttgctgcga ttcacagtgc gcgataccgg tatcggcatg acaccggaac aaatcggaaa
     1141 actcttccag tcgttcagcc aggccgatgt ttccaccacg cgcaaatacg gcggcaccgg
     1201 gctgggcctg gccatatcga agcgcctcgt ggaattgatg ggcggcgaga tatgggtgga
     1261 aagcacgccg gggcaaggca gccggttcac ttttagcatc cccttcactt tcctgcccga
     1321 tgaattcggc gcccagcccg acttgagcaa actcagggta ctggttgtgg acgacaacga
     1381 aagcgcccgg cacttgatgc tgtcttatct cgagtcgttc ggcatcgagg cggtgtcggc
     1441 cccggacagt ctggacgggc tggccgtcat cgaacatgcc gacgcaagcg ggcaagcgtt
     1501 cggctgcgtg gtgctggact ggagcatgtc cggcatgagc ggtctggaaa tggcacggcg
     1561 catcaagcac gagctgccga taaaacagcg gccaaaaatc atctacctgt ccgggcacaa
     1621 gcacaccgag atgattaacg tgtccggcgc ggcaaagctg ctgtatgcgg tcatcaacaa
     1681 acccgtcacg ccgtccgccc tgttcaacgc gctcatggcc tgcgcgtccg ggcagcgcga
     1741 tactgtccag cccttgccgg aaaacgaagc gcctcccgac ctgaccggct tgcatgtctt
     1801 gctggtcgag gacaacaagt tcaaccagca gttggcaaat gcgctgctgg tgcgcgccgg
     1861 ggtcaaggtg ggcatcgccg acgacgggct ggaagcgctg ctggcgctgc aacgggaaaa
     1921 attcgatgtc gtgctgatgg atatgcagat gccgaaaatg gacgggctgg aggcaacgcg
     1981 ccagatcagg aaaatccccg cgctggccaa actacccatc atcgcgatga ccgccaacgc
     2041 catggcaggc gaccgcgaaa cctgcctcgc cgcaggcatg aacgattaca tttccaaacc
     2101 gctgcactat caggcgatgt acgccaccat cgcccgctgg gcgcatcgcg gcgggtcgcc
     2161 gacagcccct gccgaagcgc cgcccaccgg cgaagcgccg tccatcctgg acaccgaccg
     2221 ggcaagcgcc gcaatggggg gcaaggcgct ttatctgtcc atgctggcca aattcattcc
     2281 cagccaaggc cagtcggtgc aatccatccg ggatgcgctc gccgccgacg accgcaagac
     2341 ggcggaacgt ctggcccatt cgctgaaagg catcgcggcc accgtcgggg ccgccgcgct
     2401 ggccgagtcc gccgaacgtc tcgaacaggc cctcagaacg gaagacagca aggcatatcc
     2461 ggaactgctc gaaattgcgg cgagcgaact gagactcgcg gaagcatcga tagaagccta
     2521 tctggaagaa caccggaaaa acgcttattg agtcggccat taaatagatg aaaatgccgc
     2581 gttgtaggct ggcggtgagc aacgcgaacc ccagcatagg taaggtcggc aaatgccggg
     2641 gttcgtgcct ca
//
LOCUS       DASUIV010000318         7439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_825987, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000318 DASUIV010000000
VERSION     DASUIV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825987"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFW59_11315"
     CDS             <1..1173
                     /locus_tag="VFW59_11315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5364848.1"
                     /translation="RPADPSAGSRQLEYLEQEHMIIDGHAHAFPYISNQGAYASEADH
                     LHHIQRHMTTHPQGGRRFRDNGLATGPTLWDGKTPGFEGLLDVNFRVGPYGRFEWEKD
                     GDAYYIQFFPVSLEVMEARPERMLAQMQYIGVDKAMLQFAKMYGLTNEYLSDCVRRWP
                     DKLRACTAVDEDQADQPAQIDSLRHAVRDLGLTALYFDCNGYGKSNYENHLDDAKYSP
                     FWEEVQSLGIPVLWDIRVAVWRTSHPAYLDQVMRLRRLISRFPRIRHVLTHGMPGNAF
                     NAAGEIPEDLWALLSEPTVTVEILFPILYGSSWDYPYPQARPIIRELYTRLGPTKFIW
                     GSDMPNVERNCTYLQSLNYLRNYCPFIPASGMDMILGSNADALYFSIAPAGITKQA"
     gene            1246..2052
                     /locus_tag="VFW59_11320"
     CDS             1246..2052
                     /locus_tag="VFW59_11320"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX5364849.1"
                     /translation="MLHIAMIGCGAIGAGVMALLKNDPDIRFDTVIVHRERLDAARQV
                     VSSLVPEAAVVSRLDDQGMRPDLLIECAGHQAIEEHIFAALERGIPCLVVSVGALSEP
                     GMAMRLEAAAKKGKTQVQLLSGAIGAIDALSAARIGGLDSVVYTGCKPPLAWKNTSAE
                     ALFDLDAVDAATVIFEGTARDAARLYPKNANVAATLSLAGLGFDRTQVRLLADPAVTE
                     NVHHVVAKGAFGEFKLTMRGKPMATNPKTSALTVFSVVRALGNRAHTLSI"
     gene            2098..2520
                     /locus_tag="VFW59_11325"
                     /pseudo
     CDS             2098..2520
                     /locus_tag="VFW59_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558055.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2802..3803
                     /locus_tag="VFW59_11330"
     CDS             2802..3803
                     /locus_tag="VFW59_11330"
                     /inference="COORDINATES: protein motif:HMM:NF015751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HEX5364850.1"
                     /translation="MMQIPIKRTLDRIPGGMMIVPLLLGAIITTFFPATPKFFGSFTG
                     ALFTGALPILAVFYVCMGASINLKATPYILKKGGSLFGVKVSLGIALGLIFGHFLGEA
                     PISGGMFAGLSTLAIVAAMNDTNGGLYMALMGQYGKPEDVGAYSVMSVESGPFLTMVT
                     LGVAGLSAFPWPTLVGSILPLALGMLLGHFDREMRAFLSKAVPVMLPFFAFALGAGLD
                     LNKVWQAGLLGLGMGVAVVVISGIPLYFIDRLTGGTGVAGVAAASTAGNAATVPAIIA
                     AANPAYADAAQTATITVAACVVVTAILVPMLTAWVARHKAARVAEAGAVPVTGIKPA"
     gene            3819..5114
                     /locus_tag="VFW59_11335"
     CDS             3819..5114
                     /locus_tag="VFW59_11335"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX5364851.1"
                     /translation="MTQVLIIADDLSGAADCASTFVKAGLETLVLIDLHAGQSTETEF
                     VQVISIDADTRRLPGAEAAQIHLDIFNRYSSARQLLYKKIDSTLRGNFAEELGAIIHE
                     AGMAIVAPAFPQAGRFTRNGHQFLNDTPLELTEIWRGEGIPGTAFIPGLLARHGINTE
                     SVGIAEIRQGTDRLRAVLESCANNGMQAVVCDVETDDDLRAIAQASLALSVPRFWVGS
                     AGLANHLSAAAYGAVNPVLAAPLSTGGAILTVVGSLSSVSRAQAEYLFDHGRMARIDV
                     PASVLREGQANAQWQQLRDTLGTALRNESDLLIMISMEEPVDMGEGLELCQALAMLIA
                     PLADHIGAVIATGGETARAILTAMGSTGLRLVGEVEPGVPLSVAAGIKPINVITKAGA
                     FGTRDTLMRCYETLRQARLESARSAPINTTSASLPKNAN"
     gene            5142..6122
                     /gene="pdxA"
                     /locus_tag="VFW59_11340"
     CDS             5142..6122
                     /gene="pdxA"
                     /locus_tag="VFW59_11340"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5364852.1"
                     /translation="MGDAAGVGPEVIMKSLANRDLYERCRPLVVGDVNRLREAGAIVG
                     SSLTVRAIDSVASAKFEYGMVECIDLGLIPPKYPFGALSALCGHAAYCYIERAVELAM
                     KKEIDAICTAPLNKEALHAGGHLFPGHTEILAKLTHTPEVSMMLVAPNLRVIHVTTHI
                     GLLHAIQKIEPGLVERTIARGYETLVRSGIVNPRIGVCGINPHAGENGLFGCGEEEEK
                     IIPAVQAQRALGRLVEGPLPADTLFFRAARGDFDLVVAMYHDQGHGPIKVMGLESGVN
                     ITVGLPVIRTSVDHGTAFDIAGKGIADEGSLLEALRQAIGLATRRDIARD"
     gene            6253..7095
                     /locus_tag="VFW59_11345"
     CDS             6253..7095
                     /locus_tag="VFW59_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX5364853.1"
                     /translation="MAINKTRKRHQMILGIVSSGDVNVDELSLHFGVSESTIRRDLAE
                     LVNSGRIVRTYGGAALSHDERRESSLDERIHLQRAQKIAIARLAALQIREGETIILDA
                     GTTTGALARQLGGRSLHLITNNIAALTALSNEPDITLTVLGGSLRKMSMGTVGPLTES
                     TLSRITADKVFLGADGLVAGRGLCEASQEQAALKEKMIAQASEVFVLVDASKVGFAGQ
                     QAWTPLDRRWTLITDSGATPAQLAPFQEQPNITVMVAKLGESHLDQVGTLRRCESRED
                     VCRT"
     gene            7170..>7439
                     /locus_tag="VFW59_11350"
     CDS             7170..>7439
                     /locus_tag="VFW59_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364854.1"
                     /translation="MSLSASTLSAIHQAGQGLHEAHHAVTQAVQAAAEQMVATVASQP
                     FSPESDRAYAQLRSVARLAHELQAMEEQMKALYTSAAQMVTPETPV"
BASE COUNT         1535 a         2150 c         2194 g         1560 t
ORIGIN      
        1 cggccggcag acccgtcagc gggctcccga caacttgaat acctggagca agaacacatg
       61 atcatcgacg ggcacgcgca cgctttccca tacatcagca accagggcgc ctatgcctcc
      121 gaggcggacc acctgcatca cattcagcgg cacatgacca cccaccctca gggcggccgc
      181 cgttttcgcg acaatggcct cgccaccggc cccaccttgt gggatggcaa gacgcccggc
      241 ttcgaaggac tgctggacgt caatttccgc gtcgggccct atgggcgctt cgaatgggaa
      301 aaggacggcg acgcctatta cattcaattt ttcccggtct cgcttgaagt aatggaggcc
      361 aggcccgaac gcatgctggc gcagatgcaa tatattggcg tcgacaaggc gatgctccag
      421 ttcgccaaga tgtacggtct gaccaacgag tacctgtccg actgcgtgag gcgctggccc
      481 gacaagttgc gcgcgtgcac ggccgtcgac gaggaccagg ccgaccagcc cgcgcaaata
      541 gacagcctgc gtcacgccgt gcgcgacctc ggtcttaccg cgctttactt cgattgcaac
      601 ggctacggca agagcaacta cgagaaccac ctcgacgacg cgaagtacag cccgttctgg
      661 gaagaagtgc agtcgcttgg cattccggtg ctgtgggata tccgtgtggc cgtgtggcgc
      721 accagtcacc ccgcctacct ggatcaggtg atgcgcctgc gccgcctgat cagccgcttt
      781 ccgcgcatac gccatgtgct cacccacggc atgccgggca acgccttcaa cgccgccggc
      841 gaaataccgg aggatctctg ggcactgctg agcgagccga ccgtgactgt cgaaattctt
      901 tttcccatcc tctacggcag cagctgggat tacccctatc cgcaggcacg gcccatcatc
      961 cgggagctgt acacgcgact gggtcccacc aagttcatct ggggttccga tatgcccaat
     1021 gtcgaacgca attgcaccta tttgcaatcc ctgaattacc tgcgcaacta ctgtccgttc
     1081 attccggcta gcggcatgga catgatcctc ggcagcaacg ctgacgcgct ctatttttcg
     1141 attgcgccag caggaatcac aaagcaggca tgacatgcct gcttcgacca ttgacccttg
     1201 tcgaatgggg caggaaaatc aaattgcgta tcgatggaga atggaatgct gcatattgcg
     1261 atgatcggtt gtggtgccat cggcgccggc gtgatggcgt tgctgaaaaa cgatcccgat
     1321 atccgtttcg acacggtcat tgttcaccgt gagaggctgg atgctgcgcg gcaagtggtc
     1381 tcgtcactgg tcccggaggc ggcggtcgta agtcgcctcg acgaccaggg catgcgaccc
     1441 gatttgctga tcgaatgcgc aggtcatcag gccatcgagg agcatatctt cgcagcactg
     1501 gagcggggca tcccgtgcct ggtcgtgtca gtcggcgcct tgtcggagcc cggcatggcg
     1561 atgcggctgg aagcagccgc aaagaaaggc aagacgcagg tgcagttgct ttccggcgcc
     1621 atcggcgcca tcgatgcgct atctgccgcg aggatcgggg ggctggattc tgttgtctat
     1681 accggctgca agccgccgct tgcctggaaa aacacatcag ccgaagcact gtttgacctg
     1741 gacgcggtcg atgctgccac ggtgatcttt gaaggaacag cgcgcgatgc ggcccggctc
     1801 tacccgaaaa acgccaatgt tgccgccacg ctgtcgctgg caggcctggg cttcgatcgt
     1861 acgcaggtcc gtctgctcgc agatccagcg gttaccgaga atgtgcatca cgttgtggcc
     1921 aagggtgcat tcggagagtt caaactgacc atgcgcggca agccgatggc caccaatccg
     1981 aaaacctcgg cgctgacggt gttcagcgtt gtgcgggcac ttggcaaccg cgcccacaca
     2041 ctttctattt aaaccagtca gattgacctg ctccagcgtc gatgaaagcc cggcgttcag
     2101 gcgatcggat tgacagcacc ggacttgccc ccggttcccg gcaaggcaac taccgttggc
     2161 cgcgactacg aatacgtgcg ccgcggcacg atgtagctct tggcgggtat tgacctgcac
     2221 tacccgccag aggcgatcat ccgcgtggtg ctggacaacc actcggcaca catctccaag
     2281 gagaccgtgg cccatctgag cacacggccg gggcgcttcg agtacagcca tacgccaaaa
     2341 cacggctccc ggctcaattt gatcgagtgc gccttctccg agatggcgag agccttccgg
     2401 cgccacattc gagttcagtc cgtcgatgaa ttcaaagccc gcattcttca aggcatcgct
     2461 gagattaacg cttcaccggt tgtatttcga cggcacaagt ttgacctcgg ggttgcttga
     2521 aatgtattgt tattgctgga acgaactagt tggcaacagt ttacggagct tgcaagtggg
     2581 tcaacgaatt ccggaagcag agtcaatttc gcatcagcgc caacaacgat ttctcacttt
     2641 tgcttcttca tcaaatattg ctcaaatagc gcaatgaaaa tatatttatg cgtattttgc
     2701 gcatttattc ttgcgatagt tgtaaaattc atgttggaaa tgctcatggt aagcattttc
     2761 atggttttaa atgaaaaaac aattcttcaa gatggggaaa aatgatgcag attcctatta
     2821 aacgtaccct tgaccgcatt cctggcggca tgatgatcgt ccctctgttg ctgggtgcaa
     2881 tcatcacaac atttttcccc gcgacaccaa aattcttcgg ttccttcact ggtgcattgt
     2941 tcaccggtgc attgcccatc cttgctgtct tttatgtctg catgggtgcc agcatcaacc
     3001 tcaaagctac gccctacatt ctgaaaaagg ggggctcgct gtttggcgtc aaagttagtt
     3061 tggggatcgc actgggtctc atcttcggcc attttctggg cgaagcgccg atttccggcg
     3121 gcatgttcgc tggactgtcc accttggcga tcgtcgctgc gatgaacgac accaacggtg
     3181 gcctgtatat ggccttgatg ggtcagtacg ggaaaccgga agacgttggc gcttattcgg
     3241 tgatgtcggt tgaatcggga ccattcctga ccatggtgac attgggcgtt gccggcttgt
     3301 ccgcattccc gtggcctacg ctggtcggca gcatcttgcc gctagctctt ggcatgttgc
     3361 tgggccattt tgaccgggaa atgcgtgcgt tcctgagcaa ggccgtcccg gtcatgctgc
     3421 cattcttcgc tttcgcattg ggcgccggcc tcgacctgaa caaggtctgg caagcaggcc
     3481 tactgggtct gggcatgggt gttgccgttg tcgtcatcag cggcatcccc ctgtatttca
     3541 tagatcgact gacgggcggc actggtgttg ccggtgtggc tgcagccagc acggcaggca
     3601 atgccgctac ggtgccggcc attattgccg ccgccaatcc ggcgtacgcc gatgcggcac
     3661 aaaccgcgac catcacagtc gccgcctgcg tggtggtgac agcgattctg gtgccgatgt
     3721 tgaccgcttg ggtggcccgg cataaagcgg cgcgcgtcgc cgaagcgggc gccgttccgg
     3781 taaccggtat caagccagca taaggcagag gcgcttagat gacacaagtt ctgataattg
     3841 ccgacgatct atccggcgca gccgattgcg ccagcacgtt cgtcaaggcc gggctggaaa
     3901 ctcttgtgct gatcgacctc cacgccggtc aaagtacgga aactgagttc gttcaggtga
     3961 tttcgatcga cgccgatacg cgacgtctac cgggagctga agccgcgcaa atccaccttg
     4021 atatcttcaa tcgctatagt tcggcgaggc agttgcttta caagaaaatc gactccacgt
     4081 tgcgtggcaa ttttgccgag gaacttgggg ccatcattca tgaagcagga atggcaattg
     4141 tcgccccggc atttccgcaa gccggtcgct tcacgaggaa cggccatcaa tttttgaacg
     4201 acactccgct cgaattgacg gaaatctggc gtggcgaagg cattcccggg accgccttta
     4261 ttccgggcct gttggcgcgc catggcatca acaccgaatc cgtcggcatc gccgagatac
     4321 gccagggcac ggatcgcttg cgcgccgtgc tcgaatcatg cgcgaacaat ggcatgcagg
     4381 cggtagtgtg cgacgttgaa accgacgacg acctgcgcgc cattgcacag gcatcgctcg
     4441 ctttgtcggt gccaagattt tgggtcggct ccgcggggct ggcaaaccat ttgtctgccg
     4501 ctgcgtatgg tgccgtcaac ccggtcctgg ccgctccctt aagcaccggc ggagcgatcc
     4561 tgaccgtggt tggcagtttg tcgagcgtat cgcgggccca ggccgaatac ctgttcgacc
     4621 atgggcggat ggcgcgtatc gatgtgccag ccagcgtctt gcgtgaaggt caggccaatg
     4681 cgcaatggca gcaattgcgc gacacccttg gcacggcgtt gcgcaacgag agcgacttgc
     4741 tgatcatgat cagcatggaa gagccggttg acatgggcga aggattggaa ctatgccaag
     4801 cgctggcaat gctgatcgcg ccgctggccg atcatattgg cgcagtcatt gccacgggcg
     4861 gtgaaaccgc gcgcgccata ttgaccgcga tgggctcgac gggtctgcgc ctggtcggcg
     4921 aagtcgagcc aggcgttccg ctgtccgtcg cggccggcat caagccgatt aacgttatca
     4981 ccaaggccgg cgcattcggt acgcgcgaca ccctgatgcg ctgttatgaa acgctacgcc
     5041 aggcacggct ggaatctgcg cgtagcgcac ccattaacac gacatccgca tccttaccta
     5101 agaacgccaa ttaaatgtac tcccctatca ttgcaataac catgggcgac gccgctggtg
     5161 tcggtccgga agtcatcatg aaaagcctgg cgaacaggga tctgtacgag cgatgcaggc
     5221 cgctggttgt cggcgacgtt aatcgcttgc gcgaagccgg agcgattgtc ggctccagcc
     5281 tgaccgtgcg tgcgatagat agcgtggcat cagccaagtt tgaatacggc atggtcgagt
     5341 gcatcgatct gggcttgatt ccgcccaagt atccattcgg cgcattatcg gcattgtgcg
     5401 gccacgccgc ctactgctac atcgagcgtg cagtagagtt ggccatgaag aaggaaatcg
     5461 acgcgatctg caccgcgccg ctgaataagg aagcactgca tgcggggggc catctgttcc
     5521 cgggtcatac cgagatattg gccaagctga cccatacgcc agaagtttcg atgatgctgg
     5581 tggcgccgaa cctgcgcgtg attcacgtga cgacgcatat cggcttgcta catgcgatcc
     5641 agaaaatcga gccgggtctg gtggaacgca ctattgcgcg cggctacgag acgctggtgc
     5701 gatcgggtat cgtcaatccc cgcattggcg tctgtggcat caacccgcat gcgggcgaaa
     5761 atggattgtt cggctgcggg gaggaagagg agaaaattat ccccgcagtg caagcccagc
     5821 gtgccctcgg acgcctggta gaaggtccct tgccggccga cacgctattc ttccgggcgg
     5881 cgcgtggcga cttcgatctg gtcgtcgcga tgtaccatga tcagggtcac ggcccgatca
     5941 aggtcatggg gctagagtcc ggcgtcaaca tcacggtggg cttgccggtg atccggacct
     6001 ctgtcgatca cggaaccgcg ttcgacatcg ccggtaaagg catcgctgac gaaggcagcc
     6061 tgctggaagc gctgcgtcag gcgattggcc tggccactcg ccgggacatt gctcgggact
     6121 agtgccggtg gcccaggatt gccgcgtttc atacatacaa atgccatacg ccacgcgatt
     6181 tgcgttgcac tgctttatac ctgggacggg aatcgtgtaa attaggcgcg tcaaatccca
     6241 acttcattcg tcatggccat caataaaacc agaaaacggc atcaaatgat ccttggaatc
     6301 gtctccagtg gggacgtcaa cgttgatgaa ttgagcctgc attttggcgt gtccgagtcg
     6361 acgatccggc gcgacctggc cgaactggtc aatagcggac gcatcgtgcg cacctacggt
     6421 ggtgccgctt tatcgcacga cgagcggcgc gagtcctcgc tggatgagcg catccatctt
     6481 cagcgcgccc aaaaaatagc gattgcgcgt ctggctgcat tacaaatccg cgagggcgag
     6541 accatcattc ttgatgccgg cactactact ggcgcgttgg cgcggcaact gggtgggcgc
     6601 agccttcatt tgatcacgaa caatatcgcg gcactgacag cgctgtccaa tgaacccgac
     6661 ataacgctga ccgtgctggg aggaagtctg cggaaaatga gcatgggaac ggtcgggccg
     6721 ctcaccgagt ccacgctgag caggataacg gctgacaagg tgtttctcgg cgccgacggc
     6781 ttggtggccg ggcgcggctt gtgcgaagcg tcccaggagc aagcagcgct gaaagagaaa
     6841 atgatcgcgc aggcgtctga agtttttgta ctggtcgatg cgtccaaggt tggctttgcg
     6901 gggcaacagg cctggacgcc gctggatcgt cgctggacgc tcattaccga ctccggtgcg
     6961 acgcctgcgc agttggcgcc atttcaagag cagccgaata ttacagtgat ggtggcgaag
     7021 ttgggagaat ctcatttgga tcaagtcggc accttgcgcc gctgtgaaag ccgtgaggac
     7081 gtctgcagaa cgtaaactgg gctatttaac ggaaatgatc gcgctggcgt tgaacgccaa
     7141 ccgcgatgct ttcatcccca ggagcaccca tgtcactatc tgcatcgact ctttcggcca
     7201 ttcaccaagc aggccaaggg cttcacgagg cccaccacgc tgttacacag gcggtacaag
     7261 cggccgctga acagatggtc gctactgtgg caagtcaacc gttcagcccg gaaagtgacc
     7321 gggcctatgc gcaactgcgt tctgtggcac gcctggccca tgaactgcag gccatggagg
     7381 agcaaatgaa ggccctctat acctctgcgg ctcagatggt gacaccggag acgcctgtc
//
LOCUS       DASUIV010000319        16779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_269734, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000319 DASUIV010000000
VERSION     DASUIV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 16779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16779
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269734"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFW59_11355"
     CDS             <1..185
                     /locus_tag="VFW59_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364855.1"
                     /translation="KGCEVSGIRVKVQVAYAAPQPQQRPRELSKTARNALNELGANLD
                     DSPLKQALQRMARKTG"
     gene            complement(288..1199)
                     /gene="lpxC"
                     /locus_tag="VFW59_11360"
     CDS             complement(288..1199)
                     /gene="lpxC"
                     /locus_tag="VFW59_11360"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX5364856.1"
                     /translation="MVKQRTLKGSVSVTGVGLHSGEKVTLGLRPAPVNTGVVFRRVDV
                     KPVEEIVARADLVHDTRLSTCMEQNGVRIATVEHLMSALAGLGVDNIYVDLDSAEVPI
                     MDGSAGTFIFLLQSAGIVEQSAAKKFIRIKKSIELKQGDKWVRFDPYQGYKLTFTINF
                     AHPVFANAKQHVIVDLGEESYVRDISRARTFGFMQDVENMRAQGLALGGSLDNAIVMD
                     DYRVLNADGLRFEDEFVKHKVLDAIGDLYMMGYPLIGAFSGFKSGHALNNALVRALLA
                     DEQAWEFVTFDSVEDAPAFLRLQLQAA"
     gene            complement(1262..2440)
                     /gene="ftsZ"
                     /locus_tag="VFW59_11365"
     CDS             complement(1262..2440)
                     /gene="ftsZ"
                     /locus_tag="VFW59_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5364857.1"
                     /translation="MFEIMEAETQDAIIKVIGVGGCGGNAVDHMIEQGVQGVEFIVIN
                     TDAQALKRSKARTQLQIGATITKGLGAGAKPSVGQAAAEEDRERIKELLAGANMVFIT
                     AGMGGGTGTGAAPVVAQIARELDILTVAVVTKPFAYEGNRMRFAKAGIEALHEHVDSL
                     IIVPNSKLMEVLGADVTVPEAFKAANGVLQGAVAGIAEVINVPGLINVDFADVKTVMS
                     ENGMAMMGSAIANGPDRARTAAERAIASPLLEDVDMSGARGVLVNITSSMSLKLKEID
                     DVMECVQFAAEEATVIVGSVFDEAMGDELRVTVVATGLGAALARKQAKPEVVYRGEEA
                     TYQRTGTDNEPVANPSVNYNELDTPAVMRRGRRETIDAMEKSGMDMLDIPSFLRKQAD
                     "
     gene            complement(2494..3732)
                     /gene="ftsA"
                     /locus_tag="VFW59_11370"
     CDS             complement(2494..3732)
                     /gene="ftsA"
                     /locus_tag="VFW59_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX5364858.1"
                     /translation="MSRNSKEAKNLVVGLDIGTSKIVAIVGEVGPDGALEVIGMGMHE
                     SDGMKKGMVVNIEATVGAIQRALEEAELMADCKIHEVYTGIAGSHIRGINSRGMVKIK
                     DKEVMVTDIERVIETASSISLPGDQQILHILEQEFSIDGQDGIKKPLGMSGMRLDVEV
                     HIVSGAAAAVQNIMKCIHRCGLEVNELILQPLASSKAVLEEDEKELGVCLVDIGGGTT
                     DISVFTGGAIRHTAVIPIAGDQITNDIAMALRTPTQDAEDIKIKHGCALRQLADDGAI
                     EVPGVGERGPRMLSRQTLAEVIEPRVEELYSLVQQELRRSGFEDMLSSGIVITGGSSA
                     MQGMVELGEEIFHLPVRLGIPKYVGGLSDVVKTPRMATGVGLLLYGLEHHQRNEETRM
                     QSSSFGDVLAKMKAWFQGNF"
     gene            complement(3753..4457)
                     /locus_tag="VFW59_11375"
     CDS             complement(3753..4457)
                     /locus_tag="VFW59_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX5364859.1"
                     /translation="MWDNHLLLRNIASTLFGFSVLAVLYGVAYYTVHLPGLFPLQNVR
                     MSAAPQRVVAEDVLQVVRREVNGNFFTVDIEHLRGALEQLPWVRSVSIRREFPDRLTL
                     QLEEHQALAHWNGTALVNTHGEVFSADSEELLPEFIGQEGDSAEVAQRYGEFGKQLAP
                     LDLKVAHLTLTPRHAWRVTLSNGMVLELGREEMQQRLANFVAVYPYSLAGMAASVRYV
                     DLRYRNGFALGGAFNG"
     gene            complement(4447..5388)
                     /locus_tag="VFW59_11380"
     CDS             complement(4447..5388)
                     /locus_tag="VFW59_11380"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX5364860.1"
                     /translation="MNTTQSSILDPQSFGKVAVLFGGTSAEREVSLKSGSAVLAALQR
                     GGVDAHAFDPAERNLQALRDEGYDRAFIALHGRGGEDGTVQGALELLGIPYTGSGVLA
                     SALGMDKWRTKLVWQAAGLPIPAYTLLNENSDWDAVVAELGLPLFVKPANEGSSVGIS
                     KVKTAAQLQVAYQEAAKYDKLVIAESFVGGGEYTAAILGDQALPVIKIEPANEFYDFE
                     AKYLRDDTRYLCPCGLSTEREAEMQHLAKLAFALIGGQGWGRVDFLMSEDGRPYLLEI
                     NTSPGMTDHSLVPMAARQAGIAFEQLVQKVLELSHVG"
     gene            complement(5385..6455)
                     /gene="murB"
                     /locus_tag="VFW59_11385"
     CDS             complement(5385..6455)
                     /gene="murB"
                     /locus_tag="VFW59_11385"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX5364861.1"
                     /translation="MNMSEPAQFINPLPGPLPQAGEGDKVSLREYSDASLRGELRFDV
                     PMRKHTSWRAGGVARRMYRPADLEDLLAFLRTLPEEEPLIAVGLGSNLLVRDGGLRGT
                     VLLMHGALTGLRQEADGSLYIEAGVAGAKLARFASLHNLRGAEFFAGIPGTVGGMLAM
                     NAGCYGSEIWEKVLRVQTVDRRGELHMRTPQDYQIGYRSVKRLEGRGSRIEHPTSACG
                     RGAGGEGQSSLLTPQSPEEFFVSATMKFEQGDGEVARQEIKALLAKRIASQPLNLPNA
                     GSVFRNPSGDHAARLIQQCGLKGKCIGGAQVSEKHANFIVNTGEATAADIENLINEVR
                     ATVAAQTGVQLHPEVRIIGETK"
     gene            complement(6452..7858)
                     /gene="murC"
                     /locus_tag="VFW59_11390"
     CDS             complement(6452..7858)
                     /gene="murC"
                     /locus_tag="VFW59_11390"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX5364862.1"
                     /translation="MKHKVKNIHFVGIGGSGMNGIAEVLLNLGFKVSGSDLGENVATQ
                     RLKQLGATVYLGHAEQHLTDADAVVVSTAVGKDNPEVLAARARRIPVVPRAMMLAELM
                     RLRQGIAVAGTHGKTTTTSLVASMLAKGGYDPTFVIGGKLNSSGTNARLGTGEFIVAE
                     ADESDASFLYLQPILAVVTNIDADHMETYGHDFGRLKQAFVDFVEHLPFYGMAMLCVD
                     DPNVREIMPRITKPVCTYGLSEGAQIRAVNVRHEGGQMRFTAECRQNGTPRDLDITLN
                     LAGMHNVLNALAAIAIALEVGVADDAIVAALAEFEGVGRRFQRYGEVAVKDGGSFTLI
                     DDYGHHPVEMAATLAAVRGAFPGRRLVLAFQPHRYTRTRDLFEDFVKVLCSVDALVLA
                     EVYSAGESPIVAADGRALMHAMRVSGQSEAVFVEQVADMPQAILQLARDGDVVITMGA
                     GSVGGVAAAIKQLAQDQK"
     gene            complement(7938..9062)
                     /gene="murG"
                     /locus_tag="VFW59_11395"
     CDS             complement(7938..9062)
                     /gene="murG"
                     /locus_tag="VFW59_11395"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX5364863.1"
                     /translation="MLGNGAAATTPQSSIPNPQSSACRSILIMAGGTGGHIMPGLAVA
                     DLLRAEGWQVTWLGAPGSMEAELVPKHGYEVAWVNFSGLRGKGLLRKLVLPFTLLRAM
                     GQSMAAIFRHRPDVVLGLGGYITFPGGVMAALLRRPLVIHEQNSVAGLSNRVLARMSQ
                     RVLSGFPEVLPQAVWCGNPVRASIAALDEPQQRYRARSGRLNLLVVGGSLGAKALNEA
                     LPQALALLPEEARPHVLHQTGKQHDEAVKGLYRQAGVQADVRPFLDDMAGCYADADLV
                     ICRAGALTVAELAAAGVASVLVPFPFAVDDHQTHNARFLSERGAAVLLPQGELNAEKL
                     AQLLRELDREKLLAMAQQARSVAKADAAQAVANVCKELAE"
     gene            complement(9119..10282)
                     /gene="ftsW"
                     /locus_tag="VFW59_11400"
     CDS             complement(9119..10282)
                     /gene="ftsW"
                     /locus_tag="VFW59_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX5364864.1"
                     /translation="MVSMVKTQTRPPQAQFDELLIWVLVGLLAIGLVMVYSASIATAE
                     GGKFTGHQPAYYLIRHGVFIAVGLLAGAFAFQVPVQMWQNYASWLFVAGALLLILVLV
                     PGIGREVNGSRRWLSLFVINLQPSELMKLFAVLYAASYTVRKVKLKDTFVKAFVPMFV
                     VMALVGSLLLLEPDMGAFVVICAIALCTLWLGGFNGKVFAALVVALPLAFAALIYSSP
                     YRMQRVIGFMDPWADPYGKGYQLSHALIAFGRGEWFGVGLGGSVEKLFYLPEAHTDFL
                     MAVIAEELGLAGVTAVILLFALFVVRAFQIGRHAAFLERNYSALVAQGIAVWLGVQAM
                     INIGVNVGVLPTKGLTLPFLSFGGSGVVVNCIAAAVLLRVDYENRRVARGLPV"
     gene            complement(10282..11706)
                     /gene="murD"
                     /locus_tag="VFW59_11405"
     CDS             complement(10282..11706)
                     /gene="murD"
                     /locus_tag="VFW59_11405"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5364865.1"
                     /translation="MMSLRDKSVLVLGLGETGLSLARWLSAQGARVRVADSRTNPPGV
                     DVLRRDCPQAEVRCGQFEPHLLKDIELIAISPGVPLADPFVQIAIGRGIPVVGDIELF
                     AQQLNAQSSVLNPKVIAITGSNGKTTVTSMIEHLCRAAGKDAVAAGNISPAVLDVVLA
                     RGEKQPEVWVLELSSFQLETTENLNADAATVLNISEDHLDRYSGMDGYAGAKARIFRG
                     CAVQLLNRDDLRSMSMAQQRAVSFGLDAPRNEDDWGVERDGGDIWLVQGKQRLLKASE
                     LQVAGLHNVANALAALALCRAIGLPLPALLEALRSFTGLPHRVERVAEISGVIYYDDS
                     KGTNVGATEAALKGLGRKAVVILGGEGKGQDFAPLNAVVAQHARAVVLIGRDAPLIEV
                     ALQGCGVPLQRAADMDDAVRQAAGLAQRGDAVLLSPACASFDMFRNYAHRAEVFIAAV
                     HGLQKPVLSSVEGPVLSPVEGGAA"
     gene            complement(11703..12788)
                     /gene="mraY"
                     /locus_tag="VFW59_11410"
     CDS             complement(11703..12788)
                     /gene="mraY"
                     /locus_tag="VFW59_11410"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5364866.1"
                     /translation="MLLALAQWLSVDIRFFSVFNYITLRTVLAALTALVISFMVGPAM
                     IRKLTAYKIGQSVRTDGPQTHLVKAGTPTMGGALILTAIAITTLLWGDLHNHYVWVVL
                     VTTLGFGVIGWVDDYRKVVHRNPKGLSARAKMFWQSLIALAVAAYLWNTSTMPAQTEL
                     IVPFLKFAVFPLSAFVFIVLTYLVIVGTSNAVNLTDGLDGLAILPTVMVSGALAIFAY
                     VAGNAVFAKYLGIPYIPGAGELAVFCGALVGAGLAFLWFNAYPAEVFMGDVGALALGA
                     ALGIVAVIVRQELVLFIMGGVFVVEAISVMIQVGSFKLTGKRVFRMAPLHHHYELKGW
                     KETQVVVRFWIITMLLVLIGLATLKLR"
     gene            complement(12773..14182)
                     /gene="murF"
                     /locus_tag="VFW59_11415"
     CDS             complement(12773..14182)
                     /gene="murF"
                     /locus_tag="VFW59_11415"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5364867.1"
                     /translation="MNEKGMMPLSQAAQILKGKLVGPDAHFDAISTDSRRIKAGDLFI
                     ALRGEKFDGYEFAAATLQCGAAAAMVNADSYEANSEIINPKSSILVVDDTRLALGQLA
                     AHWRRKFEVPLVAITGSNGKTTVKEMLASILRAAAGSEDAVLATKGNLNNDIGMPLTL
                     LQLNAQHRYAVIEMGMNHPGEIDYLTRIACPDVALINNASGAHLEGLGSVMAVAQAKG
                     EIFEGLSDHGTAVINADDAHASLWRGLAGTRPILEFGLERSADVSGEWQPQGSGQYLT
                     VQTPQGEFSVELQVPGAHNARNALAAATAAIALNVPLQIIAAGLQAFGGVAGRLQRKL
                     GKQGATLIDDTYNANPASMRAAIEVLAQVIGKRILVLGDMGELGEGAVAFHAEIGRAA
                     RDAGIEQLWALGELSRHAVQEFGTGARHFGHIDELTGALTRELDANTTVLVKGSRFMK
                     MERVVQACAAQEETQCCSH"
     gene            complement(14179..15663)
                     /locus_tag="VFW59_11420"
     CDS             complement(14179..15663)
                     /locus_tag="VFW59_11420"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5364868.1"
                     /translation="MFPLDELNGLNVKITRLQTDSRAVRPGDTFVAYPGEKLDGRRFI
                     EQAIEQGANAVIYDQHNFVWNEAWQVPNLAVGELRHKAGWLADAVYGKASEHLRVIGI
                     TGTNGKTSTSHWIAQVLADVGKKCALIGTLGNGFSGELQPSANTTPDAIRVHELFADY
                     LKQGACAVAMEVSSHALAQGRVNGVRFDVALLTNLSRDHLDYHGDMDSYAASKRKLFD
                     WESLRYAVLNLDDAYGASLAQELSEDAVEVVGYGMSDAALQLAERLGIRMVYGRVTEM
                     SARGLSLNIHSSWGGAQLHSKLLGRFNAENLLGVLAALLVSDVELDAAVQSLTRVTPV
                     AGRMQRLGGAEQPTVVVDYAHTPDALEKVLLTLRELNGATSDLICVFGCGGDRDRGKR
                     AMMGLVAEKFADHCIVTSDNPRSENPANIIADIIGGMSVGKHEIVPERGAAIARAIAR
                     ARQQDTVLVAGKGHEDYQEIDGVKHPFSDVAAAEHALRTWRAPS"
     gene            complement(15663..>16779)
                     /locus_tag="VFW59_11425"
     CDS             complement(15663..>16779)
                     /locus_tag="VFW59_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5364869.1"
                     /translation="IVEDAGSLHAPKPGSDIVLSLDSNVQHLAYRELENAVRENKAKA
                     GAVVVLDAKTGEVLALANYPSYNPNNRHKMTPQSMRNRAIVDLFEPGSTMKPFTVATA
                     IETGKVRPDSLIDTEHGVITVNNRKIHDSHVELEPLTVSQVIQKSSNVGSSKISLMMT
                     PAVLWQSLSDSGFGEATGSHFPGEASGVLHDPKSWRPIQQATLSYGHGLSVNLLQLAR
                     GYTVFAGDGEIKPISLLKLDAPAVGKKVFSEKTVRALRAMLELVVLPGGTAPLAQVAG
                     YRVAGKTGTAHKLENGRYINRYVASFVGFAPASDPRLIVAVMIDEPDNGQYYGGLVAA
                     PVFSRVTGGALHALNVPNDAPLDNVIAPPVEIIKEEV"
BASE COUNT         3175 a         5877 c         4618 g         3109 t
ORIGIN      
        1 acaagggctg cgaggttagc ggaatacgcg tcaaggtgca agttgcgtac gccgctcccc
       61 agcctcagca acgccctcgc gaactcagca aaaccgcacg caacgccctg aacgaactcg
      121 gcgccaacct ggacgactcg ccgctcaagc aggcgctgca gagaatggcg cgcaagaccg
      181 gctaaatcga tgcgctccgg ctggcacagc agcccgaaca gcattaacgc ggctggagta
      241 aaaaaataga cacgggatcg acgcagatac cgccgttttg accgaagtta cgcggcttgc
      301 agctgcagcc gcaggaatgc cggcgcgtct tccacgctat cgaaagtgac gaattcccaa
      361 gcctgttcgt ccgccagcaa ggcacgtacc agcgcattgt tcagcgcatg gccggacttg
      421 aatcccgaga atgccccgat caatgggtac cccatcatgt agagatcgcc gatggcatcg
      481 agcaccttgt gcttgacgaa ctcgtcctca aaacgcagac cgtcggcatt gagcacccgg
      541 taatcgtcca tgacaatggc gttatccaga ctgccaccca gcgccaaccc ctgagcgcgc
      601 atattctcga catcctgcat aaaaccgaag gtgcgcgcac gactgatgtc gcgaacatag
      661 gattcctcgc ccagatcgac aatgacatgc tgcttggcat tggcgaacac cggatgggcg
      721 aagttaatgg tgaaggtcag cttatagccc tgatagggat cgaagcgcac ccacttgtca
      781 ccctgcttca gctcaatgct tttcttgatc cggatgaatt ttttggcggc ggattgctcg
      841 acgatgcccg cagattgcag caggaagatg aaggttccgg cactgccgtc catgatcggc
      901 acttcggcgc tatcgagatc gacatagata ttgtcgaccc ccaatcccgc cagcgccgac
      961 atcagatgct cgacggtcgc gatgcgcacg ccgttctgct ccatgcaagt ggacagcctg
     1021 gtgtcgtgca ccagatcggc acgcgcgacg atctcctcca ccggcttgac gtccacgcga
     1081 cgaaacacca ccccggtatt gaccggggcg ggacgcaagc ccagcgtgac cttctcgccg
     1141 ctgtgcaagc cgacacccgt cacgctgacg gagcccttca atgtgcgctg cttaaccatc
     1201 ggtacgcctt atcaggctga aatcagggcg cgattttacc acgccgcaac ccgcctcccg
     1261 atcaatcagc ctgtttgcgc aggaacgatg ggatatccag catatccatg cccgactttt
     1321 ccatcgcatc gatggtctcg cgacggccgc gacgcatcac ggcaggcgta tccagctcgt
     1381 tgtaattcac cgacggattg gcgacaggct cgttatccgt gccggtacgc tggtaagtcg
     1441 cctcttcgcc gcgatacacc acttcaggct tggcttgttt gcgcgccaat gccgcaccca
     1501 gaccggttgc caccacggtc acacgcaact cgtcgcccat cgcctcgtcg aacaccgagc
     1561 ccacgatcac ggtcgcttct tcggcggcaa actgcacgca ctccatcacg tcgtcgattt
     1621 ccttcagctt caggctcatg ctcgacgtga tattcaccag cacaccgcgc gcaccggaca
     1681 tatccacatc ttccagcagc gggctggcga tggctcgttc ggcggcagta cgcgcacgat
     1741 caggtccgtt agcgatcgcg gaacccatca tcgccatgcc gttctccgac atcacggtct
     1801 tcacgtccgc gaagtccacg ttgatcaacc ccggcacgtt gatgacttcg gcgatacctg
     1861 ccaccgcgcc ctgcagcacg ccattggcgg ccttgaacgc ttccggcacg gtcacgtcgg
     1921 cacccagcac ctccatcagc ttggaattcg gcacgatgat cagcgaatcg acatgctcgt
     1981 gcaatgcctc gataccggct ttggcgaagc gcatgcggtt gccttcatag gcgaacggct
     2041 tggtcaccac cgccacggtc aggatatcca gctcgcgtgc gatctgcgcg acgacaggcg
     2101 ccgcaccggt gccggttccg ccgcccatac ctgcggtgat gaataccatg ttcgcgccgg
     2161 cgagcagctc cttgatgcgc tcgcgatcct cttctgctgc ggcttgcccg accgatggct
     2221 tggcgcctgc gcccagcccc ttggtgatgg tcgcgccgat ctgcaactgg gtgcgcgcct
     2281 tgctgcgctt gagcgcctgc gcatcggtat tgatgacgat aaactcaacg ccctgcacgc
     2341 cctgttcgat catgtgatcg accgcattgc cgccgcagcc gcccacgcct atcaccttga
     2401 tgatcgcgtc ctgagtttca gcttccatga tttcaaacat cgttctctcc ttttcgtaat
     2461 tacaaaatta accctgaacc caacctgaac ctgctaaaaa tttccctgga accatgcttt
     2521 catctttgcc agcacatcgc cgaaagaact cgattgcata cgcgtctcct cgttgcgttg
     2581 atgatgttcc aaaccgtaca gcagcagtcc gaccccggtc gccatgcgcg gcgtcttcac
     2641 cacatcggac aatccgccga catatttggg aatacccagg cgcaccggca gatggaaaat
     2701 ttcctcgccc aactccacca tgccctgcat cgcactgctg ccgccggtga tcacgatgcc
     2761 ggaggacaac atgtcctcga agccgctgcg gcgcagctcc tgctgcacca gcgaatacaa
     2821 ttcttcaacg cgcggctcga tcacctccgc cagcgtctga cgcgacaaca tgcgcgggcc
     2881 gcgctcaccc acacccggca cctcgatcgc gccatcgtcg gccaattggc gcaatgcgca
     2941 gccgtgcttg atcttgatat cctctgcatc ctgcgtcggc gtgcgcagcg ccatcgcgat
     3001 gtcgttggtg atctggtcgc cagcgatcgg aatgacagcg gtatgccgga tcgcgccgcc
     3061 ggtgaacacc gagatatccg tcgtgccacc gccgatatcg accaggcaca cgcccagctc
     3121 cttctcgtct tcctccagta ccgccttgga ggacgccagc ggctgcagga tcaactcgtt
     3181 cacctccagc ccgcagcggt gaatgcactt catgatgttc tgcaccgcag cagccgcgcc
     3241 gctgacgata tgcacctcga catccagtcg catgccgctc atgcccagcg gcttcttgat
     3301 cccgtcctga ccgtcgatac tgaactcctg ctcgaggata tgcaggatct gctgatcgcc
     3361 cggcaggctg atcgaactgg ccgtctcgat cacgcgctcg atgtcggtga ccatcacttc
     3421 cttgtccttg atcttcacca tgccgcgcga attgatgccg cgaatgtggc tgcccgcgat
     3481 gccggtatag acctcgtgga tcttgcagtc ggccatgagt tcggcctctt ccagtgcgcg
     3541 ctggatcgcc cccaccgtcg cctcgatgtt gaccaccatg cccttcttca tgccgtcgga
     3601 ttcgtgcata cccatgccga tcacctccag cgcaccgtcc ggaccgacct cgccgacgat
     3661 agccactatc ttggatgtgc cgatatccag accgaccacc agattcttgg cttctttgct
     3721 atttctgctc atcgtttgct ccccactgcc tcttaaccgt taaacgcgcc gcccagcgca
     3781 aagccattgc ggtaacgcaa atccacatac ctcaccgagg ctgccatccc tgccagactg
     3841 tacggataga ccgccacaaa attcgccagg cgctgctgca tctcttcgcg ccccaactcc
     3901 agcaccatgc cgttgctcag cgtcacacgc catgcatggc gcggcgtcaa cgtaagatgg
     3961 gccactttca aatccagcgg cgccagttgc ttgccgaatt cgccataacg ttgcgcgacc
     4021 tctgcagaat caccctcctg cccgataaac tccggcaaca gttcttcgct atcggcgctg
     4081 aacacctcac cgtgggtgtt taccaacgct gtgccgttcc agtgagccag cgcctgatgc
     4141 tcttccagct gcagcgtcaa tcggtccggg aactcgcgcc ggatactgac gctgcgcacc
     4201 caaggcagct gttccagcgc cccccgcaaa tgctcgatat ccacggtgaa gaaatttccg
     4261 ttcacctcgc ggcgcaccac ttgcagcaca tcttctgcca ccacccgttg cggcgccgca
     4321 ctcatgcgca cgttctgcaa cgggaacaaa ccgggcagat gtacggtgta gtacgccacg
     4381 ccgtacagca ccgccagcac actgaaaccg aacagtgtgc tggcgatgtt cctcagcagg
     4441 aggtggttat cccacatgcg aaagttccag caccttctgc accagttgtt cgaatgcaat
     4501 tcccgcctgc cgcgccgcca tcggcaccag actgtgatcg gtcatgcccg gcgaagtgtt
     4561 gatctccagc agatagggcc ggccgtcttc actcatcagg aaatccacac gcccccagcc
     4621 ttggccaccg atcagcgcga aggccagctt cgccagatgc tgcatctccg cctcccgctc
     4681 cgtactcagg ccacaggggc acagatagcg cgtgtcatcg cgcagatact tggcttcgaa
     4741 atcgtagaac tcgttcgccg gttcgatctt gatcaccggc agcgcctgat cgcccaggat
     4801 cgccgccgtg tattcgccgc cgccgacaaa actctcggcg atcaccagct tgtcgtactt
     4861 ggcggcctcc tgataggcga cctgcaactg ggcggccgtc ttgaccttgc tgatgccgac
     4921 gctggaaccc tcgttagccg gtttcacgaa cagcggcaaa ccgagctctg ccaccacagc
     4981 atcccagtcg ctgttctcgt tcaacagcgt gtaggccgga atcggcaacc ctgccgcctg
     5041 ccacaccagc ttggtgcgcc acttgtccat acccagcgcc gaagccagca cgccgctgcc
     5101 ggtgtaaggg atgccgagca gttccagtgc tccctgaacc gtaccgtcct caccgccgcg
     5161 accgtgcagt gcgatgaacg cgcggtcata accttcgtcg cgcaacgcct gcagattgcg
     5221 ctctgccgga tcgaacgcgt gcgcgtccac gccgccgcgc tgcaacgcag ccagcacggc
     5281 cgagccgctc ttcagcgaaa cctcgcgctc ggcggacgtt ccgccgaaca gcaccgccac
     5341 cttgccgaag gattgaggat cgaggattga ggattgagtc gtattcattt cgtttcacca
     5401 ataattctta cttctggatg caactgcacc ccggtctgcg ctgctaccgt cgcgcgcact
     5461 tcgttgatca ggttctcgat atccgccgcc gtcgcctcac ccgtattcac gatgaaattg
     5521 gcatgcttct ccgacacctg cgcgcccccg atgcatttgc ctttcagtcc gcattgctgg
     5581 atcagtcgcg ccgcatggtc gcccgacgga ttgcggaaca ccgatcctgc attcggcaaa
     5641 ttcagcggct ggctggcgat gcgtttggcc agcaaggcct tgatctcctg ccgcgcaacc
     5701 tctccgtcac cctgctcgaa cttcatcgtc gccgagacaa aaaattcctc cggggattga
     5761 ggagtgagga gtgaggattg cccctctccc ccagcccctc tcccgcaagc ggaggtgggg
     5821 tgctcgatcc tcgatcctcg gccctccaac ctcttcaccg agcggtagcc gatctggtag
     5881 tcctgcggtg tgcgcatgtg caattcaccg cgccggtcca ccgtctgcac gcgcagcacc
     5941 ttctcccaaa tctcgctgcc gtagcagccg gcattcatcg ccagcatgcc gcccaccgtg
     6001 ccgggtatcc cggcgaaaaa ttccgcaccg cgcaggttgt gcagtgaggc gaagcgcgcc
     6061 agctttgcgc ccgccacccc ggcctcgata tagagactgc catccgcttc ctgccgcaaa
     6121 cccgtcaatg cgccgtgcat caacagcacc gtgccgcgca gaccgccgtc gcgcaccagc
     6181 agattgctgc ctaggcccac cgcgattagc ggctcttctt ccggcaatgt gcgcaagaac
     6241 gccaacagat cctccagatc ggccgggcga tacatccgcc gcgccacacc gcccgcacgc
     6301 cagctggtgt gtttgcgcat cggcacgtcg aagcgcaatt cgcctctcag actcgcatcg
     6361 ctgtattcgc gtagcgaaac cttatccccc tctcccgctt gcgggagagg gccggggaga
     6421 gggttgatga actgtgccgg ttcgctcata ttcatttctg atcctgcgcc agctgtttga
     6481 tcgctgcggc cacaccgccg accgaaccgg cgcccatggt gatcaccaca tcgccgtcgc
     6541 gcgccagctg caggatcgcc tgcggcatgt ccgccacctg ctcgacgaat accgcctcgc
     6601 tctgacccga cacgcgcatc gcatgcatca gcgcgcgtcc gtcggccgcg acgatcggcg
     6661 actctcccgc cgagtacacc tcggccagca ccagcgcatc gacgctgcac agcaccttca
     6721 cgaagtcttc gaacaggtcg cgcgtgcgcg tgtagcgatg gggctggaat gccagcacca
     6781 gtcgcctgcc cggaaacgcg ccgcgcaccg ccgccagcgt cgccgccatc tccaccgggt
     6841 gatggccgta atcgtcgatc aacgtaaagc tgccgccgtc tttgaccgca acctcgccgt
     6901 aacgctggaa gcgcctgccc actccttcga attcggctaa tgcggcaacg atggcgtcat
     6961 cggccacgcc cacttccagc gcgatggcga tcgcggccag cgcgttcagc acgttgtgca
     7021 taccggccag attcagcgtg atgtccagat ccctgggcgt accgttctgg cggcactcgg
     7081 cggtgaaacg catctgcccg ccctcatggc gcacgttgac cgcgcgtatc tgcgcgccct
     7141 cgctcaggcc ataggtgcag accggcttgg tgatgcgcgg catgatctcg cgcacattgg
     7201 gatcgtccac gcacagcatc gccatgccgt agaacggcag atgctcgacg aaatcgacga
     7261 acgcctgctt caaccgccca aagtcatggc cgtaggtctc catgtgatcg gcatcgatgt
     7321 tggtgacgac cgcgaggata ggctgcagat agaggaagga cgcatccgac tcgtctgctt
     7381 ccgcgacgat gaattcgccc gtccccagcc gcgcgttggt gccgctgctg ttaagtttgc
     7441 cgccgatcac gaaggtcgga tcgtagccgc ccttggccag catgctggcg accagcgagg
     7501 tcgtcgtggt cttgccgtgt gtgcccgcaa ccgcgatccc ctgacgcaga cgcatcagct
     7561 ccgccagcat catcgcgcgc ggcaccaccg gtatgcggcg cgcacgcgcg gccagcacct
     7621 ccggattgtc cttgcccacc gccgtggaca ccaccaccgc gtcggcatcg gtcagatgct
     7681 gctcggcatg gcctaagtac accgtcgcgc cgagctgctt caaccgctgc gtcgcgacat
     7741 tctcgccaag atcggaaccg ctgaccttga agccgagatt gagcagcacc tctgcgatgc
     7801 cgttcatgcc cgatccgccg atgccgacga aatgaatatt tttgacctta tgtttcatat
     7861 ttctccccca actctgggat gtttggctcc ggcataagcc atgcctccgc cgcaacaagt
     7921 cgctacgcgc cgtattgtca ttcggccagc tccttgcata cgtttgcgac ggcctgcgcc
     7981 gcatcggcct tcgccacgct gcgcgcctgt tgcgccatcg ccagcaattt ttccctgtcc
     8041 agttcgcgca gcaactgcgc caacttctcc gcattcagct caccctgcgg caacagcacc
     8101 gccgcgccgc gctcactcag aaagcgcgcg ttatgggtct ggtggtcatc caccgcgaac
     8161 gggaacggca ccagcacgct cgccacgccc gccgccgcca gctccgcgac cgtcagcgca
     8221 ccggcgcgac agatcaccag atcggcatcg gcataacagc ccgccatgtc gtcgaggaag
     8281 ggacgcacgt cagcttgcac accggcctgc cggtacagcc ccttcaccgc ctcgtcgtgc
     8341 tgcttgccgg tctggtgcag cacatgcggc cgcgcctctt ccggcaacaa ggccagcgcc
     8401 tgcggcaacg cctcgttgag cgccttcgcg cccagactgc cgcccaccac cagcaggttc
     8461 agcctgccgc tgcgcgcgcg atagcgctgc tgcggctcgt ccagcgcggc gatgctcgcg
     8521 cgcaccggat tgccgcacca caccgcctgc ggcaacacct ccggaaatcc gctcagcacg
     8581 cgctgcgaca tgcgcgccag caccctgttg ctcaatccgg ccaccgaatt ctgctcgtgt
     8641 atcaccagcg gacggcgcag caatgccgcc atcacaccgc cggggaaggt gatgtaaccg
     8701 cccagcccca gcaccacgtc gggacgatgg cggaagatcg ccgccatgct ctgccccatc
     8761 gcgcgcagca gcgtgaacgg caacaccagc ttgcgcagca accccttgcc gcgcagcccg
     8821 gagaaattca cccacgcgac ctcatagcca tgcttcggca ccagctcggc ctccatgctg
     8881 cccggcgctc ccagccaggt gacctgccag ccttctgcgc gcaacagatc ggcaaccgcc
     8941 agccccggca tgatgtgccc gccggttccg cctgccatga tcaggatgga cctgcaagcc
     9001 gaggattgag gatttgggat cgaggattga ggggttgtcg cggcagcgcc attccccagc
     9061 atgccgcttg gcggagcatc actctcgatc ctcaatcctc gatcctcaat tctgtgattc
     9121 ataccggcag ccctctagca acacgccgat tctcgtagtc gacacgcaaa agcaccgctg
     9181 cggcgatgca gttcaccacc acgccgctgc cgccgaagct caagaaaggc agggtcagac
     9241 ccttggtcgg cagcacgccc acattcacgc cgatgttgat catcgcctgc acgcccagcc
     9301 acacggcgat gccctgtgcg accagcgcgg aatagttgcg ctccagaaaa gcggcatgcc
     9361 ggccgatctg gaaggcgcgg acgacgaaca atgcaaacag caggatcacc gccgtcaccc
     9421 ccgccaatcc caattcctct gcaatcaccg ccatcaggaa atcggtatgc gcctccggca
     9481 gatagaacag cttctccacg ctgccgccca gccccacgcc gaaccattcg ccgcgcccga
     9541 aggcgatcag cgcatggctc agctggtaac ccttgccgta cgggtctgcc cagggatcca
     9601 tgaaaccgat cacgcgctgc atgcggtagg gcgaggaata gatcagcgcc gcgaaggcca
     9661 gcggcaaagc cacgaccagc gcggcgaaca ccttgccgtt gaagccgccc agccaaagcg
     9721 tgcacagtgc gatcgcgcag atcaccacga atgcgcccat gtccggctcc agcagcagca
     9781 ggctgccgac cagcgccatc accacgaaca tcggcacgaa ggccttgacg aaggtgtcct
     9841 tcagtttcac cttgcgcacc gtatagctgg cggcatacag caccgcgaac agcttcatca
     9901 gttcggaggg ctgcagattg atgacgaaca gcgacagcca gcgacggctg ccgttcacct
     9961 ctcggccgat gcccggcacc agcaccagga tcagcagcaa ggcccccgcc acgaacagcc
    10021 aggacgcata gttctgccac atctgcaccg gcacctgaaa ggcgaaggcg cctgcaagca
    10081 ggcctaccgc gatgaacacc ccgtggcgta tcaggtagta ggccggctga tgaccggtga
    10141 acttgccgcc ttccgccgtg gcgatcgagg ccgagtagac catcaccaag ccgatcgcga
    10201 gcaagccgac cagcacccag atcagcaatt cgtcgaattg ggcctgcggc ggacgggtct
    10261 gggtcttgac catggagacc attacgcagc ccctccttcg acagggctca ggacaggccc
    10321 ttcgaccgag ctcaggacag gcttttgcaa gccgtgcacc gccgcgatga acacctcggc
    10381 gcgatgtgcg tagttgcgga acatatcgaa actcgcgcag gccggcgaca gcagcacggc
    10441 gtcgccgcgc tgcgccaatc cggctgcctg ccgcaccgca tcatccatgt ctgccgcgcg
    10501 ctgcaatggc acgccgcaac cctgcagcgc cacctcgatc agcggcgcat cgcgcccgat
    10561 cagcaccacc gcgcgcgcat gctgcgccac gacggcgttg agcggcgcga agtcctgccc
    10621 cttgccttcg ccgccgagga tcaccacggc cttgcgcccc agccccttga gcgccgcttc
    10681 ggtcgcgccg acattggtcc ctttggaatc gtcgtaatag atgacgccgg agatctccgc
    10741 cacgcgctcg acgcgatgcg gcaatcccgt gaagctgcgc aacgcctcaa gcagtgcggg
    10801 cagcggcagg ccgatcgcac ggcacagtgc cagtgcagcc agcgcattgg cgacgttatg
    10861 cagcccggcc acctgcagct cgctcgcctt cagcaagcgc tgcttgccct gcaccagcca
    10921 gatgtcgccc ccgtcgcgct ccacgcccca gtcgtcttcg ttgcgcggcg catccagccc
    10981 gaagctcacg gcgcgctgct gcgccatact catgctgcgc agatcgtcgc ggttcagcag
    11041 ttgcacggcg cagcccctga agatgcgtgc cttggccccc gcgtagccat ccataccgct
    11101 gtagcggtcc agatgatcct cgctgatgtt cagcaccgtc gccgcatcgg cattcaaatt
    11161 ctcggtcgtc tcgagctgga agctggacag ttccagcacc cacacctccg gctgtttctc
    11221 cccgcgcgcc agcaccacgt ccagcaccgc aggcgagata tttccggccg cgaccgcatc
    11281 ctttcctgcg gccctgcaca ggtgctcgat catgctggtg accgtggtct tgccgttgga
    11341 accggtaatg gcaatcactt taggattgag gaccgaggat tgagcattga gctgttgtgc
    11401 gaacagctcg atgtcgccga ccaccgggat gccgcgcccg atggcgatct gcacgaaggg
    11461 atcggccagc ggcacgccgg gactgatcgc gatgagttcg atgtctttca acaaatgcgg
    11521 ttcaaactgg ccgcagcgga cctccgcctg agggcaatcg cggcgcagca catcgactcc
    11581 gggaggattg gtacggctgt cagcgacgcg cacgcgcgca ccctgcgcgc tcaaccagcg
    11641 cgcaagcgac agcccggtct caccgaggcc gagcaccagc accgacttgt ctctcaggct
    11701 catcatctca atttcagcgt cgccaacccg atcaatacca gcagcatggt gataatccag
    11761 aatctgacca cgacctgggt ctccttccag cctttcaatt cgtagtggtg atgcaacggc
    11821 gccatgcgga acacgcgctt gccggtgagc ttgaaggagc cgacctggat catcaccgag
    11881 atcgcctcga ccacgaacac gccgcccatg atgaacagca ccagttcctg ccgcacgatc
    11941 accgccacga tgcccagcgc ggcgcccaat gccagcgcgc cgacatcgcc catgaagacc
    12001 tcggcgggat aggcgttaaa ccacaggaac gcgaggcccg cgccgaccag cgcgccgcag
    12061 aacaccgcca gctcgcccgc gccggggatg tagggaatac cgagatattt cgcaaacacc
    12121 gcgttacccg cgacataggc gaagatcgcc agcgcaccgc tcaccatcac cgtcggcaga
    12181 atcgccaggc catccaggcc atcggtcaga ttcaccgcat tgctggtgcc gacgatgacc
    12241 agataggtca gcacgatgaa cacgaatgcc gacaacggaa acaccgcgaa tttcagaaag
    12301 ggcacgatca gttcggtctg cgcgggcatc gtggaggtat tccacagata ggccgcaacc
    12361 gccagtgcga tcagcgactg ccagaacatc ttggccctgg ccgacaagcc cttgggattg
    12421 cgatgcacca ccttgcggta atcgtcgacc cagccgatca caccgaaacc cagcgtggtg
    12481 accagcacca cccagacata gtgattgtgc agatcgcccc acagcagcgt ggtgatcgca
    12541 atagcggtca ggatcaatgc gccgcccatg gtgggcgtgc ccgccttcac cagatgggtc
    12601 tgcggcccgt cggtgcgtac cgactggccg attttgtagg cggtcagctt tcgtatcatc
    12661 gccgggccga ccatgaacga gatcaccagc gcggtcagcg cggccagcac cgtgcgcagc
    12721 gtgatgtaat tgaacacgct gaaaaaacga atatccacgg acagccattg ggctaatgcg
    12781 agcagcattg tgtttcctcc tgcgccgcgc aagcctgcac gacgcgttcc attttcataa
    12841 agcgcgaacc cttcaccagc acggtggtgt tcgcatccag ttcccgcgtc agcgcgcccg
    12901 tcagctcatc gatatgcccg aagtggcgcg cgcccgtgcc gaattcctgc accgcatgac
    12961 ggctgagttc gcccagcgcc caaagctgtt cgatgcctgc atcgcgcgcc gcgcggccga
    13021 tctcggcgtg gaatgcaacc gcgccctcgc ccagctctcc catgtcgccc agcaccaata
    13081 tgcgcttgcc gataacctgc gccagcactt cgatcgccgc ccgcatcgag gccggattgg
    13141 cgttataggt atcgtcgatc agcgtcgctc cctgcttgcc gagcttgcgt tgcagacgtc
    13201 cggcgacgcc gccgaaggct tgcaagcctg cggcgatgat ctgcagcggc acattcaagg
    13261 ctatcgctgc ggtcgccgcc gccagcgcat tgcgcgcgtt gtgcgctccg ggcacctgca
    13321 gctcgacgct gaattcaccc tgcggggttt gtaccgtcag gtactgcccg cttccttgcg
    13381 gctgccactc tcctgaaacg tcagccgacc gttccagacc gaattcaagt atcggacgcg
    13441 ttccggccag tccgcgccac agcgaggcgt gcgcatcgtc ggcgttgatc accgccgtgc
    13501 cgtggtcgct cagaccttca aagatttcgc ccttggcttg tgccacggcc atcaccgagc
    13561 ccagcccctc cagatgggct ccgctggcgt tgttgatcag cgcgacatcg gggcaggcga
    13621 tacgggtcag atagtcgatc tcgcccgggt gattcatgcc catttcgatc acggcataac
    13681 gatgctgcgc attcagttgc agcaaggtca gcggcatgcc gatgtcgtta ttgaggttgc
    13741 ccttggtcgc cagcaccgca tcctcgctgc ctgctgcggc gcgcaagatg ctggccaaca
    13801 tttccttgac cgtggtcttg ccgttgctgc cggtgatggc caccagcggg acttcaaatt
    13861 tccggcgcca atgcgcggcc agctgcccca gtgcaagacg ggtgtcgtcc actacaagaa
    13921 ttgaggattt tggatttatg atttctgaat ttgcctcgta gctgtcggcg ttgaccatcg
    13981 ccgccgctgc accgcactgc agcgtggcgg cggcaaactc gtagccgtcg aacttttcgc
    14041 cgcgcagcgc gatgaacaga tcgcctgcct tgatcctgcg gctgtcggtg gagatcgcat
    14101 cgaaatgcgc atccggcccg accagcttgc ccttcaaaat ctgcgcggct tgcgacagcg
    14161 gcatcatgcc cttctcgttc atgacggcgc cctccatgtc cgcagcgcat gctcggcggc
    14221 ggcgacatcg ctgaacggat gtttcacgcc atcgatttcc tgatagtcct cgtgcccttt
    14281 acccgccacc agcacggtgt cctgctggcg cgcacgtgcg atggcccggg cgatagccgc
    14341 gccgcgctcg ggcacgatct cgtgcttccc gacgctcatg ccgccgatga tgtcggcaat
    14401 gatattggcc ggattctcgc tgcgcgggtt gtcgctggtg acgatgcaat gatcggcgaa
    14461 cttctcggcg accaggccca tcatggcgcg tttgccgcga tcccgatccc ctccgcagcc
    14521 gaacacacag atcaaatcgc tcgttgcgcc attcaactcg cgcagcgtca gcaacacctt
    14581 ttccagtgca tcaggcgtgt gcgcataatc caccaccacg gtcggctgct ctgcgccacc
    14641 caaccgctgc atgcgtccgg ccaccggcgt cacgcgggtc agcgattgca cggccgcatc
    14701 gagttcgaca tcgctgacca gcagcgccgc cagcacgccc agcagattct ctgcgttgaa
    14761 gcgcccgagc agcttgctgt gcaactgcgc accgccccag cttgaatgaa tgttcaggct
    14821 caggccgcgc gcactcatct cggtcacccg tccgtacacc atgcgtatgc ccagacgctc
    14881 ggccaactgc aaggccgcat cgctcatgcc gtaaccgacc acctcaaccg catcctcgct
    14941 tagctcctgg gccagactcg cgccatatgc gtcgtccaga ttcagcaccg cgtagcgcag
    15001 cgactcccag tcgaaaagct tgcgcttgct cgccgcatag ctgtccatgt cgccgtggta
    15061 atcgagatga tcgcggctca gattggtcag caatgccaca tcgaagcgca cgccgttgac
    15121 gcgcccctgc gccagcgcat gagaggacac ttccatcgcg actgcgcaag caccttgctt
    15181 aagatagtcg gcgaacagtt catgcacgcg gatcgcatcc ggcgtggtgt tcgcgctagg
    15241 ctgcaactcg ccggaaaaac cgttgcccag cgtgccgatc agcgcgcatt tcttccccac
    15301 atcggcgagc acctgcgcga tccagtgact ggtcgaggtc ttgccgttgg ttccggtgat
    15361 gccgatcacg cgcagatgtt cagaggcttt tccataaacg gcatcagcca gccaacccgc
    15421 cttgtggcgc aactcgccca ccgccagatt cggcacctgc caggcttcgt tccagacgaa
    15481 attatgctgg tcgtagatca ccgcattcgc gccctgttcg atggcctgct cgatgaagcg
    15541 ccgtccgtcc agcttctcgc ccggataggc gacaaaggta tcgcccggcc gcaccgcgcg
    15601 actgtcggtc tgcagccgcg tgatcttcac attcagccca ttcaattcat cgagagggaa
    15661 catcacacct cctccttgat gatctctact ggcggcgcga tcacgttatc cagcggcgca
    15721 tcgttcggca cgttgagcgc atgcagtgcg cccccggtca cccggctgaa cactggcgct
    15781 gcgaccagcc cgccgtaata ctggccgtta tccggctcgt cgatcatcac cgccacgatc
    15841 aggcgcggat cggaggccgg cgcgaaaccg acgaaagacg cgacataacg gttgatgtag
    15901 cgcccgttct ccagcttgtg cgccgtaccc gtcttgcccg ccacgcggta acccgctacc
    15961 tgagccagtg gcgcggtgcc gcccggcagc acgaccagct ccagcatggc gcgcaatgcg
    16021 cgcacggtct tctcggagaa caccttcttg cccaccgccg gggcatccag cttgagcagc
    16081 gaaatcggct tgatctctcc gtcaccggca aagacggtgt agccccgtgc cagttgcagc
    16141 agattcaccg agaggccgtg accataggac agcgtcgcct gctggatggg gcgccagctc
    16201 ttggggtcat gcagcacgcc cgacgcctcg cccgggaaat ggctgcccgt cgcctcgccg
    16261 aaaccgctgt cggacaggct ctgccacagc accgctggcg tcatcatcag ggatatcttg
    16321 gacgaaccga cgttgctgga tttctggatc acctgcgata cggtgagcgg ctccaattcc
    16381 acgtgcgagt cgtggatctt gcgattgttc accgtgatca cgccatgctc ggtatcgatc
    16441 aggctatcgg gacgcacctt gccggtctcg atcgcagtcg ccaccgtaaa cggcttcatc
    16501 gtcgagccgg gctcgaacag atcgacgatg gcgcggttgc gcatcgactg cggagtcatc
    16561 ttgtgacgat tgttcgggtt ataggaggga tagttggcca gcgccagcac ctcgcctgtc
    16621 ttggcatcca gcaccaccac cgcgcccgcc ttggccttat tctccctgac ggcgttttcc
    16681 agttcgcggt aggcaagatg ctgcacgttg ctgtccaggc tgagcacgat gtcgctgccc
    16741 ggcttgggcg cgtgcagact gccggcatcc tcgacgata
//
LOCUS       DASUIV010000320         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_456976, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000320 DASUIV010000000
VERSION     DASUIV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456976"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFW59_11430"
     CDS             <1..939
                     /locus_tag="VFW59_11430"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX5364870.1"
                     /translation="TSAYLALTGDKFLLWSRSGQSFLMFAVTDRYWVAMGDPVGIPEE
                     QEELAWELRTLADRHGAYAAFYQVGTPRIPLYLDLGLTLLKLGKEARVPLPTFSLEGN
                     QRASLRQSCNRLMREGLSFEIVPASGVDAILPELQIISDRWMMDKQTREKRFSLGFFN
                     ADYLRRCTIALVKKEGRIIAFANLWELESRDELSMDLMRYEPGTANGLMDYLIVSLLL
                     WGKAQGYRWFNLGMAPLSGLEQHPLAPLWNKIGNTIFRLGNEFYNFDGLYQYKSKFDP
                     VWQPRYLAAPAGLSMASALLSVNTLISGNLKGVFSK"
     gene            936..2330
                     /locus_tag="VFW59_11435"
     CDS             936..2330
                     /locus_tag="VFW59_11435"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HEX5364871.1"
                     /translation="MTRILIAALLVLITGQALAADVRESTDSFGPFGTLHIYTTAAQP
                     KHMTLFVSGDGGWNLGVIDMARSLAQQDSMVVGIDITHYIRQLDTGHEKCAYPAAHFE
                     ELSQYLQKKYRFPHYTLPVLAGYSSGATLVYTTLAQSPPNTFAGGISLGFCPDLKSAK
                     PMCKGSGTLTGRPDKKLGYIYDPAPALAARLYVLQGDADQVCSTPDTKAFLSKINGAE
                     MIELAKVGHGFAVQKNWMPQFEQAFQKIVGAQPAHAAPAHAVDALSDLPLVELPATGS
                     SATLAVMVSGDGGWAGIDKQIAEALNRDGIGVIGLNSLQYFWQKKDPDIAGRDLTRIL
                     NHYGSAWGAEKLILVGYSSGADTLPFMVSRLPPELRSRVKIVALLGLGQEANFEFHVS
                     DWLFDAKGAYQVIPEVQKLNGLKVLCIYGEDEHDSACKLLGSKDATAIEMKGGHHFSG
                     DYDKLANTILDHSR"
     gene            2475..3503
                     /locus_tag="VFW59_11440"
     CDS             2475..3503
                     /locus_tag="VFW59_11440"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HEX5364872.1"
                     /translation="MDSIAVEPDGSFVSKSVKGMTYWYFQTPQVSGQRKQVFVGKETP
                     ELLARIEAARLAKADAANILNERKRLVAMLSAGGATMEKGRPARIIASMAKAGMFDGG
                     GVLVGSFAFACYGNMLGVTFASSLSRTEDMDFSVQREIQIGMSRNLGEELQRVEPSLK
                     LPQQIRPSAKPFEMIASDGFKVEFLTTRDGPADKFPVPIEQLSIHAQPLEYMDYLIEN
                     NQPAVLLYGAGVPVRVPDPARFALHKLAVSQLRPSGTKSKADKDLRQAEAIIGVLLED
                     NPGLLLLAADAINERADLMSNLVRAGATLLPAPMKEEIENAVHGKEWDTGAGTTRRRK
                     TKPDARKR"
BASE COUNT          795 a         1094 c         1111 g          679 t
ORIGIN      
        1 acctcggctt atctggcatt gaccggcgac aagtttctgc tatggagccg tagcggccag
       61 agtttcctga tgttcgccgt cacggaccgt tactgggtcg cgatgggcga cccggtgggg
      121 ataccggaag aacaggaaga attggcatgg gaactgcgca cgctggccga ccggcacggt
      181 gcgtatgccg ctttttatca ggtgggcacg ccgcgcatcc cgctctatct cgatctcggg
      241 ctcacgctgc tgaagctcgg caaagaggcc agggtgccgc tgcccacctt cagcctagag
      301 ggaaaccagc gcgcgagcct ccgccagtcc tgcaaccggc tgatgcgcga aggcctgtcg
      361 ttcgagatcg tgccggcctc cggcgtggac gcgatactcc ccgagctgca aatcatttcc
      421 gaccgctgga tgatggacaa gcagacccgc gagaaacgtt tctcgctcgg cttcttcaac
      481 gcggactatc tgcgtcgctg cacgattgcg ctagtcaaaa aagaggggcg catcatcgcc
      541 tttgccaacc tgtgggaact ggaaagccgg gacgagcttt cgatggacct gatgcgctac
      601 gagccgggaa ccgccaatgg cttgatggat tatctgatcg tctcgctgct gttgtggggc
      661 aaagcacagg ggtatcgctg gttcaatctc ggcatggcgc cgctttcggg gctggagcag
      721 catccgcttg cgccgctgtg gaacaagatc ggcaacacca tcttccgttt aggcaacgag
      781 ttctacaact tcgatggcct gtaccaatac aagagcaagt tcgacccggt ctggcagccg
      841 cgctatctgg ccgcgcctgc cgggctgtcg atggcgtcgg ctctgttgtc cgtcaatacg
      901 ctgatctcgg gaaatctgaa aggggtgttt tcaaaatgac cagaatactg attgcggcgc
      961 tgctggtgct gataacgggc caggcgctgg cagccgatgt gcgggaaagc accgacagct
     1021 tcgggccgtt cggcacgctg catatctaca cgactgccgc acaaccgaaa catatgacgc
     1081 tgttcgtctc cggcgacgga gggtggaatc tgggcgtgat cgacatggca cgatcgctgg
     1141 cgcaacagga cagcatggtg gtcggcatcg acatcacgca ctacatccgc cagctcgata
     1201 cgggccatga aaaatgcgcc tatcctgcgg cgcatttcga ggagctcagc cagtatctgc
     1261 aaaagaaata ccgtttcccg cattacacgc tgccggtgct ggcgggctat tcgtccggcg
     1321 cgacgctggt ctataccacc cttgcgcaat cgccgccgaa cacctttgcg ggcggcatca
     1381 gcctgggctt ctgccccgac ctgaagtccg ccaaaccgat gtgcaaaggc agcggcacac
     1441 tgaccggcag gcccgacaaa aaactgggct atatctacga tcccgctccc gcgctggctg
     1501 ccagactcta cgtgctgcaa ggcgatgccg accaggtgtg cagcacgccc gacaccaagg
     1561 catttctgag caagatcaac ggtgcggaaa tgatagaact ggcaaaggtc gggcacggtt
     1621 ttgccgtgca aaaaaactgg atgccgcaat tcgagcaggc tttccagaag atcgtcggcg
     1681 cgcagccagc ccatgcggca ccggcgcatg cggtcgatgc gctaagcgac ctgccgctgg
     1741 tcgaacttcc cgcgacaggc agcagcgcta cgctggcggt gatggtttcc ggagacggcg
     1801 gctgggccgg catcgacaaa cagatcgccg aagccttgaa cagggatggc atcggcgtga
     1861 tcggcctgaa ctcgctgcaa tatttctggc agaagaaaga ccctgacatc gcgggccggg
     1921 acctgacacg catcctgaat cattacggca gcgcatgggg tgcggagaag ctaattctgg
     1981 tcggctactc gtctggggcg gacacgctac ccttcatggt cagccgcctg ccgccagaac
     2041 tgaggtcccg cgtgaagatc gtcgcgttgc tgggattggg acaggaagcc aatttcgagt
     2101 tccacgtgtc cgactggctg ttcgacgcca aaggggcata ccaggtcatc cccgaggtgc
     2161 agaaactgaa cggcctgaag gtgctgtgca tctacggcga ggatgaacac gacagcgcat
     2221 gcaagctgct gggcagcaag gatgccacgg ccatcgagat gaaaggcggc catcatttct
     2281 cgggggatta cgacaagctg gccaatacca tcctcgacca ttccagataa gagggtgtcc
     2341 cggattttgt gtaaacggag tttccaattt ggatggcaga gttatactca caaaatacaa
     2401 atctgagtat aaccatgaca aaccgacttc ccgacaactg cgtcgccctg tatgccgatc
     2461 tccagcaaaa ggtcatggac agcatcgccg tcgagccgga cggctcgttc gtatccaaat
     2521 cggtcaaggg catgacctat tggtacttcc agacgcctca agtctccggc cagcgcaaac
     2581 aggtattcgt cggcaaggaa acgccggaac tcctcgccag aatcgaagcg gcgaggctcg
     2641 ccaaagcaga cgccgccaat atcctgaacg agcgcaagcg cctggtggcg atgctctccg
     2701 ccggcggcgc gacgatggaa aaggggcggc ccgcccggat catagccagc atggcgaaag
     2761 ccggaatgtt cgacggcggc ggcgtgctgg tcggctcgtt cgcgttcgcc tgctacggca
     2821 acatgctggg cgttacgttc gcttccagcc tgtcgcggac cgaggacatg gatttctccg
     2881 ttcaaaggga gatacaaatc ggcatgtcgc gcaacctcgg cgaggagtta cagcgcgtgg
     2941 aacccagcct caaattgccg caacaaattc ggccatcggc gaaaccattc gagatgatcg
     3001 cgagcgacgg attcaaggtc gaattcctga ccaccagaga cggtcccgcc gacaagtttc
     3061 cggtgccgat agagcagctc tccatccacg cccagccgct cgaatacatg gactacctga
     3121 tcgaaaacaa tcagcccgcc gtcctcctgt acggcgcagg cgtgccggtg cgcgtacccg
     3181 acccggccag attcgccctg cacaaactgg cagtgtcgca actgcgcccg tcgggcacga
     3241 aaagcaaagc cgacaaggac ctgcgccaag ccgaagcgat catcggcgtt ctgctcgaag
     3301 acaaccccgg cctgctcctg ctggctgccg acgcaatcaa cgagcgcgca gacctcatgt
     3361 ccaacctcgt gcgcgccgga gccacgctgc tcccggcccc gatgaaggaa gaaatcgaga
     3421 acgccgtgca tggcaaggaa tgggataccg gggcggggac gacacggagg cggaagacga
     3481 agccggatgc tcggaagcgc taagatctcg gtcggtgtgg cggtgtttgc gggctggtgg
     3541 agggtgtccc ggattttgtg taaacggagt ttctctattg ctgcggcatc cggtcttcga
     3601 actggatagt aaaccgattt agcgcagcct tccaatcccg aatcggcatc gtccatttct
     3661 tgcggatgtt gttcaacgc
//
LOCUS       DASUIV010000321         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_456996, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000321 DASUIV010000000
VERSION     DASUIV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456996"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFW59_11445"
     CDS             complement(<1..365)
                     /locus_tag="VFW59_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5364873.1"
                     /translation="MYQQHFGLREPPFGLTPDTSFFFACTSYQEALNTLLVAARNGEG
                     FIKITGEVGNGKTMLCRKFLATLNQGWQATTPIGTQDQAAPAASAPAASAPRFVTAYI
                     PNPYLEPRSLLQALAEEFRV"
     gene            complement(392..2053)
                     /locus_tag="VFW59_11450"
     CDS             complement(392..2053)
                     /locus_tag="VFW59_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX5364874.1"
                     /translation="MRVLLCLGAGLLLAGCAIPGVKNQTADSIKNEMNQAAQASMQGQ
                     SDAVDKALLPPLSANLPKVESKPLEAKFDLTVSGTPVSQVFMAIVSGTRYSMLLHPDV
                     SGNISLNLKDVTVFEALETIRELYGYDYKVDGTRIYIQPLSLQTRIFQVNYLTGQREG
                     KSSLRVSSGSLGDTAAGTLATPTVNGVTNRDSSKVTMTSSADFWDELGKALTAIVGTE
                     KGRSIVISPMTGVIVLRAMPDEIKNVAAYLKASQISIERQVILEAKIVEVQLNDGFQS
                     GVNWGIFNSGLNSGSSVGQIGNGGLLAPRIAGVPSVIGGGGSLTAIPGKDMLNAVTPA
                     GGTLFGLAFQTGNFAALLSFLESQGNVHVLSSPRIATLNNQKAVLKVGTDEFFVTEIT
                     GGTQATATIPGTAPTVKVQPFFSGIALDVTPRIDQNNEIILHVHPSVSAVTTVNKIVS
                     MGGVAGNFNLPLASSSVSETDSIVRARDGQIVAIGGLMRQATTNDSSGLPGLSKMLFG
                     QTNVSTQKRELVILLKPTVVDNDKDWSDDITRSRDRVNGLLGNKQ"
     gene            complement(2050..2493)
                     /locus_tag="VFW59_11455"
     CDS             complement(2050..2493)
                     /locus_tag="VFW59_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364875.1"
                     /translation="MAFSLKRYGIALLALALALALSGQSALAESLSDPTRVPAAIADP
                     VASGAAVAEQTTGLQSVIISKSRRTAIIDGETVELGGKHGDAKLIEVREGSVVLMGAQ
                     GRQVLTLFPAVKITRKDIQAKPKLHDRKAKASKHKTRPVARKEEK"
     gene            complement(2478..>2697)
                     /locus_tag="VFW59_11460"
     CDS             complement(2478..>2697)
                     /locus_tag="VFW59_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agglutinin biogenesis protein"
                     /protein_id="HEX5364876.1"
                     /translation="SAGGVPRLIFRHAVQITVRGSYLELLNYLAALEKLPTQMLWGEV
                     KFAVERHPDAVLTVTLSTLSMDKTWLSV"
BASE COUNT          488 a          852 c          788 g          569 t
ORIGIN      
        1 acgcggaatt cttccgccag cgcctgcaac aaactgcgcg gctcgaggta aggattaggg
       61 atgtaggccg tgacgaagcg cggcgcggat gcggcgggtg cggatgcggc gggcgcggct
      121 tggtcctgcg tgccgatcgg cgtggtcgcc tgccagccct gattcagcgt ggcgagaaac
      181 ttgcggcaca gcatcgtctt gccgttgccc acctcgccgg tgatcttgat gaatccctcg
      241 ccgttgcgcg ccgccaccag caaggtgttg agcgcctcct gatagctggt gcaggcgaag
      301 aaaaaactgg tgtcgggagt cagcccgaag ggcggttcac gcaagccgaa gtgttgttga
      361 tacatatttg ctttgtaccg gcgctaacag gttattgctt gttgcccagc aggccgttaa
      421 cgcggtcgcg gctgcgcgtg atgtcgtccg accagtcctt gtcgttatcc accacggtgg
      481 gtttgagcag gatcaccagt tcgcgcttct gcgtgctcac attggtttgc ccgaacagca
      541 tcttcgacaa gcccggcaga ccggagctgt cgttggtcgt cgcctgccgc atcaggccgc
      601 cgatcgcgac gatctgaccg tcgcgggcgc gcacgatgct gtcggtttcc gacaccgagc
      661 ttgaggccaa cggcaggttg aaattcccgg ccacgccgcc catgctcacg atcttgttga
      721 cggtcgtcac ggcgcttacg gaggggtgca catgcaggat gatttcgttg ttctggtcga
      781 tgcgcggcgt cacgtccagc gcgatgccgg aaaagaacgg ctgcacttta acggtcggcg
      841 cggttcccgg tatcgtagcc gtggcctggg ttccgccggt aatttcggtc acaaaaaatt
      901 cgtccgtgcc gaccttcagc accgccttct ggttgttcag cgtcgcgatg cgcgggctgg
      961 acagcacatg cacattgccc tgcgattcga ggaagctcaa cagcgccgca aaattgccgg
     1021 tctggaacgc caggccgaac agcgtgccgc cagcgggtgt tactgcattt aacatatcct
     1081 tgcccggtat ggccgtcagc gagccgccgc cgccaatcac ggaaggcacg cccgcaatac
     1141 gcggagccag caggccgccg ttgccgatct gccccaccga tgagccgcta ttcagcccgc
     1201 tgttgaatat gccccagttc acgcccgact ggaaaccgtc gttgagctgc acctcgacga
     1261 tcttcgcttc gagtatcacc tggcgctcga tggaaatctg cgacgccttg agataggccg
     1321 cgacattctt gatctcgtcc ggcatcgcac gcaacacgat caccccggtc atcgggctga
     1381 tgacgatgct gcggcccttc tcggtgccga ctatggcggt cagcgccttg cccagctcat
     1441 cccagaaatc cgcactggaa gtcatcgtca ctttgctgct gtcgcggttt gtcacaccat
     1501 tcaccgttgg cgtcgccagc gtacccgctg cggtgtcgcc gagggagccg gaggatacgc
     1561 gcaacgacga cttgccttcg cgctgcccgg tcagatagtt cacctgaaaa atccgcgtct
     1621 gcaacgagag cggctggatg tagatgcgcg tgccatcgac cttgtaatcg tagccgtaca
     1681 gctcgcgtat cgtctccagc gcctcgaaga ccgtcacatc cttcaggttc agcgatatgt
     1741 tgccgctgac atcgggatgc agcagcatgc tgtagcgcgt accggaaacg atcgccatga
     1801 atacctggct caccggcgtg ccgctcaccg tcaaatcgaa tttggcttcc agcggcttgc
     1861 tttccacctt gggcagattg gccgacagcg gcggcagcaa agccttgtcc accgcatccg
     1921 actggccctg catgctcgcc tgcgcagcct ggttcatctc gtttttgatg gagtccgccg
     1981 tttggttctt gactccgggg atagcacagc ctgccagcag cagcccggct cccaggcata
     2041 ataaaactct catttttcct ccttgcgtgc gaccggcctg gttttatgtt ttgaggcctt
     2101 cgccttgcgg tcatgcaatt tcggtttggc ttgaatgtct tttctggtga tctttacagc
     2161 ggggaacagc gtcaacactt gccggccttg cgcccccatc agcacaacac tgccctcgcg
     2221 gacttcgatc agtttcgcgt cgccgtgctt gccacccagc tccacggtct cgccgtcgat
     2281 gattgcggta cggcgcgact tggaaatgat gaccgactgt aacccggtcg tttgctcggc
     2341 gaccgccgcg cctgacgcta ccggatcggc aatggcggcc ggcacgcggg tagggtcgct
     2401 caaactctcg gcgagggctg actggcctga caatgccaga gccaacgcca gagccagcaa
     2461 tgcaatgccg tagcgcttca aactgaaagc catgttttat ccatactcag ggtagacagc
     2521 gtcacggtca gcaccgcatc gggatgccgc tccacggcaa acttcacttc gccccacagc
     2581 atctgcgtcg gcagtttttc cagcgcggcg aggtaattca acagttccag atagctgccc
     2641 cgcacggtga tctgcaccgc atgccggaaa atcaaccttg gcacgccacc ggcgcta
//
LOCUS       DASUIV010000322        11312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_827199, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000322 DASUIV010000000
VERSION     DASUIV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827199"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VFW59_11465"
     CDS             complement(<1..1191)
                     /locus_tag="VFW59_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX5364877.1"
                     /translation="MPELSHAPDMLDSATRTTGDVFPVNTTRKIIVWFSAATLLVALG
                     MTASFWAFSQMKEAAEARKHARIVIISAGEWLSALKDAETGQRGYLLTGDEAFLEPYL
                     AVKSILAPRLEALRQITRIPAADKHLDAIAPLMEKKLAEMSQAIALRRNHDMAAVLAI
                     VGGGQGKLLMDAIRAEMHDVIQIEEDMLAQRDAEFQSKMLRLFILIVTASLFTLLLAL
                     AFAWMLYRESRQRLKNLVHQETQHFLRIQEETNKQLQQTNVSLQISEEKLAVTLDSIG
                     DAVLTTDAEGRITLLNPLAEQLTGWTQAAAAGHPVEHVFQIINKETRHPATIPVMSTL
                     THGTIQGLANHTVLIARDGSECDIADSCAPIRDRAGQVIGAVLVFRDVSREYAVQRAL
                     NDQQF"
     gene            complement(1198..5655)
                     /locus_tag="VFW59_11470"
     CDS             complement(1198..5655)
                     /locus_tag="VFW59_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5364878.1"
                     /translation="MSKKTQDSNSRNVAAKTAAALPEFTQQGDSLHAAALQHAMFNSA
                     NFSCIATDANGIIQLFNVGAEHMLGYTADDVLNRMTPADTSDPQEVIDRGKTLSIEFA
                     TPIAPGFESLVYKASRGIEDIYELTYIRKDGSRVPAVVSVTALRDEQNTIIGYLLIGT
                     DNSARKRAEEALLKVGALQNAIFNSANFSSIATDAKGVIQIFNVGAERMLGYTAAEVM
                     NKITPADISDSQEIIARAEALSTELGTPITPGFEALVFKAARGIEDIYELTYIRKDGS
                     RFPAVVSVTALRDAQGIIIGYLLIGTDNSARKRAEEALLKAGALQSAIFNSAHFSSIA
                     TDAKGVIQIFNVGAERMLGYAAADVMNKITPADISDPQEVIARAAALSAELGTAITPG
                     FEALVFKASRAIEDIYELTYIRKDGSRFPAIVSVTALRDALNEIIGYLLIGTDNTARK
                     QAEEALLKAGALQNAIFNSANFSSIATDANGVIQIFNVGAERMLGYTAAEVMNKITPA
                     DISDPQEIIERARALSTELSTPITPGFEALVFKATRGIEDIYELTYIRKDGSRFPAVV
                     SVTALRDAQGEIIGYLLIGTDNTARKQVEAEQAKLDQRLRDQQFYTRSFIESNIDAIM
                     ATDPYGIITDVNKQTEVLTASTRDELIGSPFKNNFTDPDRAATGIKRVLNEKKVTDYE
                     LVARARDGKETVVSFNATTFYDRDRNLQGVFVAARDITERKRLDIELENAKSVAERAN
                     LAKSDFLSSMSHEIRTPMNAIIGMSYLALKTELTPRQRDYINKIQGSGRHLLGIINDI
                     LDFSKIEAGKLTIEHVEFDLEKVLDNMANLIAEKAATKGLELVFDIDKNVPVKLIGDS
                     LRLGQILINYCNNAVKFTEHGEIDIIIRLKEQTDDDILIYCAVRDTGIGLTQEQIGRL
                     FQSFSQADTSTTRKFGGTGLGLVISKNLVELMGGEVGVDSEIGKGSTFWFTARLGKSA
                     EQQRGLVLTGSLHGKRVLVVDDNENARLVLSDMLTHMSLQVDKVESGKAAIVAVEHAV
                     AQGMPYEIVFIDWKMPDIDGIETAKRIKELPLSPLPHLAMVTAYGREEVIRGAEDVGI
                     EDVLIKPVSASILFDAVVNILGGINTPRLNIEAPTETFERLATIKGARILLVEDNDLN
                     QEVATGLLLDAGFFVDLAENGQIALNKLNITDFDMVLMDMQMPVMDGVTATREIRKQQ
                     RFRDLPIVAMTANAMQGDRDRCIAAGMNDHVAKPIEPEDLWHALLKWIKPQQSTIARV
                     QPQVTQEVTLPSDIDGLDITNGLRRVLGKTSLYLSMLRKFVAGQKDFAAEMVKALEAG
                     DRETAERLAHTLKGVAGNIGASAIQAQAAQLESALRESASRDEINASLEVLKHQLDYF
                     ITQLEQKLPDLPEGQTIKVDPKMLKAVCDQLKMLLLHDDSEAVDVVQENADLLRAAFP
                     EHYRKIDDAIHAFDFNAALLSLKAASASSEQAASAGSGQAAPAGTFTQE"
     gene            complement(5946..7388)
                     /locus_tag="VFW59_11475"
     CDS             complement(5946..7388)
                     /locus_tag="VFW59_11475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5364879.1"
                     /translation="MLKVIYDQLISPTDAGVAACHFDVWHQEALLNPKALLIAILNSS
                     HFMNIATDTKGEIRVFNAGAELMLGYAAAEVINKKTPADLFDRQELLARAQALGNAGM
                     LGFDALVFRAFRGTEDVYELTYIRKDGSRLPAIVSVTALRDAEAAIIGYLLIGTDNTA
                     QKQVEAVQKQLNRNLSDQQFYTRSLIESNIDALMATDPTGIITDVNKQMEAITGCARE
                     ELIGSASKSYFTDPERADAGIRQALSEKRICDYELTVRARDGKETVVSYNATTFYNRD
                     GVLQGVFAAARDITERKLAEERIVRLAFHDELTGLPNRRLLTDRLAQAMAASKRSGRY
                     GALMFLDLDNFKTLNDTHGHSVGDLLLIEVARRISSCVREVDFVARFGGDEFVVILGE
                     LDMDMTASTRQSNFVAEKIRVALGNPYELKVQLGDKTEKSIGHLCTSSIGMVLFLDHD
                     VGTEEIIKWADTAMYWAKEAGGNSIRIFDY"
     gene            complement(7628..8458)
                     /locus_tag="VFW59_11480"
     CDS             complement(7628..8458)
                     /locus_tag="VFW59_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364880.1"
                     /translation="MICEQEILNAKILIVDDQQANVTLLEQMLQDADYRNVSSTMDPY
                     TVCALHRDQHYDLILLDLVMPGMDGFEVLECLKEIEAEDYLPVLVVTAQPSHKLHALE
                     AGAKDFIAKPFDLIEVKTRIHNMLEVRLLYKKLKKYNEELEQTVQLRTAELRESEARF
                     RRLTELSSDWYWEQNADGHFTKISGPVLEMLGIWIDDALVQSRDDQDASWVESERKML
                     EENLAARRPFLDFVFSRISHGGVRRYFMVSGEPTFDASGRYTGYRGTGKDVTETMCAK
                     "
     gene            complement(8460..9914)
                     /locus_tag="VFW59_11485"
     CDS             complement(8460..9914)
                     /locus_tag="VFW59_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364881.1"
                     /translation="MTRKTDHGVAGQPDATVAHDIAVKKQLLDQMREEYALLIEAAGT
                     PPDAEARATDASDVGTTLLRAVNERLFINAMNAQEQTEQLQTDNIHLIRAKCTAESAN
                     LAKSDFLSRMSHELRTPLNSILGFSQLLAAGSPPLSDVQASRVKQISEAGWYLLDLIN
                     ELLNLALIESGKLSLSLGAVSLNAAMNDSRTLIEPDAELHGIQLTFPPFDAPCFVRAD
                     PGRLKQVLINLLSNAIKYNREHGTVEVKLTVMPERVRIGIKDTGIGLTAAQQAQLFQP
                     FNRLGQEAGSVEGSGIGLVVSKQLVELMGGTIGVESIPGVGSEFWIELGREHVPQAQV
                     DNRENMERRALRYGKQKTLLYVEDNPANLALVEQIIGQHKDLRLLSTRDPSLGIAFAR
                     AYLPDVIVMDINLPGISGIEIMNILRKDPATMHIPVVALSANAMPGDIEKALQNGFFR
                     YLTKPINIDEFLNVLDDSLKFLETGSDSTHKPDE"
     gene            complement(9931..>11312)
                     /locus_tag="VFW59_11490"
     CDS             complement(9931..>11312)
                     /locus_tag="VFW59_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX5364882.1"
                     /translation="SFNLIAGTPGSGKTTLAHQIMFALAAPNHRALFFTVLGEPALKM
                     LRYQQQFPFFDFNKVNESIRFVNLSSDLLRGDFDLVLARISKEVEDYAPSLVFVDSFR
                     SVVRSAKQNEQGETELQRFVQQLGMKMTSWQATTFLIGEYLEPESKSSPIFTVADGIL
                     WLSQNLHRNSMVRKMQVVKMRGQGQASGLHTFRISDQGIQIFPRAIMRHALPHESAIA
                     SYGEDERVPMGVPGLDAMLGGGLPSGYSLLVVGPSGSGKTILATEFLAEGVRRGETGV
                     IAAFEKSPSQLIGNKKLYPLVQSGQIGVIDTRSLDLSIDETLHDLIEMIHRMKAKRVV
                     IDSLSGFELALAPEFSEDFRGSLYRMIAELTGMGVTILMTSELEDRYTDLRFSPFGSA
                     FLADAIIVQRYIEISGQLKRAFSVVKVRGSAHDKDIRLFDITDEGIVIGAALSGYDGI
                     MSGRPTVSS"
BASE COUNT         2015 a         3262 c         3443 g         2592 t
ORIGIN      
        1 gaactgctgg tcattcaggg cgcgctgcac cgcatattcc cggctgacat cgcggaatac
       61 cagcacggcg ccgatcacct gcccggcgcg gtcgcgaatc ggcgcgcaac tgtcggcgat
      121 atcgcattcg ctgccatcgc gcgcgatcag tacggtatgg ttggctaggc cctgtatcgt
      181 tccgtgcgtc agggtgctca tgaccgggat ggtggcgggg tggcgcgtct ctttgttgat
      241 gatttggaaa acatgctcta ccggatggcc ggctgcggct gcctgtgtcc agccggtaag
      301 ctgttcggcc agcgggttca gcagcgtgat gcgcccttcg gcatcggtgg ttaacaccgc
      361 atcgccgatg gaatcgagcg tgacggcaag tttttcctcg ctgatctgca gggagacatt
      421 ggtctgttgc agttgtttgt tggtctcttc ctgaatccgg agaaaatgct gtgtttcctg
      481 atgcacaaga tttttcaggc gctgccgtga ttcgcgatag agcatccagg cgaaggccag
      541 tgccagcagc agggtgaaca ggctggcggt gacgatcagg ataaacagac ggagcatctt
      601 cgattggaac tcggcgtcgc gttgcgccag catgtcctcc tctatctgga tgacgtcatg
      661 catctcggca cgaatcgcat ccatcaggag cttgccctga ccgccgccca cgatggccag
      721 tacggcggcc atgtcatggt tgcggcgcaa ggcaatggcc tgtgacattt ccgctaattt
      781 cttttccatc aggggggcga tggcatccag atgtttgtcg gcggcgggga tccgggtgat
      841 ttggcgcagc gcctccaggc ggggggcgag gatgctttta accgccagat acggctccag
      901 gaaggcctcg tctccggtca gcaggtagcc gcgctggccg gtctcggcat ctttcagtgc
      961 ggacagccat tcaccggcgc tgatgatgac aatgcgggcg tgcttgcgtg cctcggcggc
     1021 ttctttcatc tggctgaatg cccagaatga tgccgtcatc cccagcgcaa ccagtagcgt
     1081 tgccgcagaa aaccagacga ttatttttcg ggtcgtattc acaggaaaga catccccggt
     1141 cgtgcgggtg gccgaatcaa gcatatcggg ggcatgagat agctcgggca tggctcatca
     1201 ttcctgagtg aacgtgcctg ccggagcggc ctgtcctgag cctgccgaag cggcctgttc
     1261 tgagcttgcc gaagcggctt tcagcgacag gagtgccgcg ttaaagtcga aagcgtgtat
     1321 cgcatcgtcg atcttgcgat agtgttccgg aaatgccgcg cgcagcagat ccgcattctc
     1381 ctggacgaca tccaccgcct ccgagtcatc gtgaagcagc aacatcttca gttggtcgca
     1441 gaccgctttg agcatcttcg gatcgacttt tatggtctga ccttccggaa ggtccggcag
     1501 cttttgctca agctgggtga tgaaatagtc gagttgatgt ttcagcacct caagcgaggc
     1561 gttgatttca tcgcgggagg cgctctctct gagcgcgctc tcgagttgcg ccgcctgagc
     1621 ttgtatggct gaggcgccga tattgccggc tacccccttc agggtgtggg caagccgttc
     1681 ggcagtctcc cggtcgccgg cttccagagc cttgaccatt tccgccgcga aatctttttg
     1741 ccctgccacg aattttcgca gcatcgacag gtagagagac gtcttgccga gcacgcggcg
     1801 caagccattg gtgatgtcca gcccgtcgat atcggacggc agcgtgactt cttgcgtgac
     1861 ctgcggctga acccgtgcga tggtggactg ctgcggcttg atccatttca gcagcgcatg
     1921 ccacaggtct tccggttcga tcggtttggc aacgtgatcg ttcatgcccg ccgcgataca
     1981 gcgatcgcga tcgccctgca tcgcattggc ggtcatcgcc acgataggca gatccctgaa
     2041 gcgttgctgt ttgcggattt cccgggtggc ggtcacgccg tccatcaccg gcatctgcat
     2101 gtccatcagc accatgtcga aatcggtaat gttgagtttg ttcagcgcga tctggccgtt
     2161 ctcggccaga tcgacaaaga agccggcatc gagcagcagg ccggttgcca cctcctgatt
     2221 caaatcgttg tcttccacca gcaggatgcg cgcgcccttg atggtggcta gtcgctcgaa
     2281 ggtttctgtc ggcgcctcga tgttgagacg gggcgtattg atgccgccca gaatgttgac
     2341 cacggcatcg aacagtatcg aggcgctgac cggcttgatc agcacatcct cgatgccgac
     2401 gtcttccgct ccccggatga cttcctcgcg accataggcg gtcaccatcg ccaggtgcgg
     2461 caggggactg agcgggagct ccttgatccg tttggccgtt tcgatgccat cgatgtcagg
     2521 cattttccag tcgatgaaca cgatctcata gggcatgccc tgtgccacgg catgttccac
     2581 ggcgacgatg gccgccttgc ccgattcgac cttgtcgacc tgaaggctca tgtgggtgag
     2641 catgtcgctc agcaccagcc gggcgttctc attgtcgtcc accaccagga cccgcttgcc
     2701 gtgcaggctc ccggtcagca ccaggccgcg ctgttgctcg gcgctcttgc ccaggcgagc
     2761 ggtgaaccag aaggtgctgc ccttgccgat ttcgctgtct acgccgacct cgccgcccat
     2821 cagttcgacg aggttcttgg agatgaccag ccccaggccg gtgccgccga attttcgggt
     2881 ggtcgaggtg tccgcctgtg aaaagctctg gaacaaccgg cctatctgtt cctgggtcag
     2941 gccgatgccg gtgtcgcgca cggcgcagta gatcaggatg tcgtcgtcgg tctgctcttt
     3001 gagacggatg atgatgtcga tctcgccatg ctcggtaaat ttgacggcat tgttgcagta
     3061 gttgatgaga atctgtccca gccgcaacga atcgcctatc agcttgacgg gcacattctt
     3121 gtcgatgtca aaaaccagtt ccagcccctt ggtcgcggct ttctcggcaa tcaggttagc
     3181 catattgtcg agcaccttct cgagatcgaa ctcgacatgt tcgatcgtca gttttccggc
     3241 ttcgattttg gaaaagtcga gaatgtcgtt gatgatgccg agcagatgcc gtccggagcc
     3301 ctggatcttg ttgatgtagt cgcgctggcg cggtgtcagc tcggtcttca gggcgagata
     3361 ggacatgccg atgatggcgt tcatgggcgt gcgtatctca tggctcatgc tggacaggaa
     3421 atcggatttg gcgagattgg cccgttccgc cacagacttg gcattttcca gttcgatatc
     3481 cagacgtttg cgttccgtga tgtcgcgtgc ggcgacaaac accccctgca gattcctgtc
     3541 gcggtcgtag aaggtggtcg cattgaagga caccaccgtt tccttgccgt ctctggcgcg
     3601 ggcaaccagc tcgtagtcgg tgaccttctt ctcgttcagc acccgtttga ttcccgtcgc
     3661 ggcgcggtcg ggatcggtga agttgttttt gaacggagag ccgatcagtt cgtcccgcgt
     3721 ggatgcggtg agcacctcgg tctgtttgtt gacatcggtg atgatgccgt acgggtcggt
     3781 cgccatgatc gcgtcgatgt tcgactcgat aaacgagcgt gtatagaact gctggtcgcg
     3841 caggcgctga tccagtttcg cctgctccgc ctccacctgc ttgcgcgcgg tattgtcggt
     3901 tccgatcagc aggtagccga tgatctcgcc ttgcgcatcg cgcaatgccg tgaccgacac
     3961 caccgccggg aagcggctgc cgtccttgcg gatataggtc agttcgtaga tgtcctcgat
     4021 gccgcgtgtc gccttgaaca ccagcgcctc gaaacccggc gtgatcggcg tcgacaactc
     4081 ggtgctcagc gccctggcgc gctcgatgat ttcctgcgga tcggaaatgt cggccggggt
     4141 gatcttgttc atcacttcgg ctgcggtgta gcccagcatg cgttctgcgc cgacgttgaa
     4201 gatctggatc acgccgtttg catcggtggc gatgctggag aagttcgcgc tgttgaagat
     4261 cgcgttctgc aatgccccgg ccttgagcag cgcttcttcc gcctgcttgc gtgcggtgtt
     4321 gtcggtgccg atcagcaggt agccgatgat ttcgttcagt gcatcgcgca atgccgtgac
     4381 cgacacgatg gccgggaagc ggctgccgtc cttgcggatg taggtcagct cgtagatgtc
     4441 ctcgatggcg cgcgaggcct tgaataccag cgcctcgaag cccggcgtga tggctgtgcc
     4501 gagttcggcg ctcaatgcgg cggctcgcgc gatcacttcc tgcgggtcgg agatgtcggc
     4561 gggggtgatc ttgttcatca catcggccgc cgcgtagccc agcatgcgtt ccgcaccgac
     4621 gttgaagatc tggatcacgc ctttcgcatc ggtggcgata ctggagaaat gcgcgctgtt
     4681 gaatatcgcg ctctgcaacg cgcccgcctt gagcagcgct tcttccgcgc gcttgcgcgc
     4741 gctgttgtcg gtgccgatca gcaggtagcc gatgatgatg ccctgcgcgt cgcgcagcgc
     4801 ggtcaccgac accaccgccg ggaagcggct gccgtctttg cggatatagg tcagctcgta
     4861 gatgtcttcg atgccgcgcg ccgccttgaa taccagggct tcgaagccgg gggtgatcgg
     4921 cgtgccgagt tcggtgctca acgcctcggc gcgcgcgatg atttcctgcg aatcggaaat
     4981 gtcggcgggg gtgatcttgt tcatcacttc ggccgcagtg tagcccagca tgcgttccgc
     5041 gccgacgttg aagatctgga tcacgccctt cgcatccgtg gcgatgctgg agaagttggc
     5101 gctgttgaag atcgcgttct gcaaggcgcc gactttgagc agcgcttctt ccgcgcgctt
     5161 gcgcgcgctg ttgtcggtgc cgatcagcag atagccgatg atcgtattct gctcgtcgcg
     5221 caacgcggtc accgacacca ctgccgggac gcggctgcca tccttgcgga tataggtcag
     5281 ctcgtagatg tcctcgatgc cgcgcgaggc tttgtacacc aacgactcga atcccggcgc
     5341 aatcggggtc gcgaactcga tgctcagggt ctttcctcgg tcaatcacct cttgcgggtc
     5401 ggacgtgtcg gccggggtca tcctgttcag cacgtcgtcg gcggtgtagc ccagcatgtg
     5461 ctcggcgccg acattgaaca gttggatgat gccgttggca tcggtagcga tacaggagaa
     5521 gttggcgctg ttgaacatcg catgctgcaa ggctgcggca tgaagtgaat cgccttgttg
     5581 cgtgaattca gggagggctg ccgctgtttt tgcggcgacg ttccgggaat tggagtcctg
     5641 tgtttttttg gacatgtcct gtctctgtgt gaaaagcgta tgagtatggc gggtgcatgg
     5701 cctgtgaatc cggcccaatc tcagctgtcc gcttgttcat gtcgaacaaa atatctgcgc
     5761 ctggtcggca ggtgtcgcgg gaggaaatga gatggaagtt gagttcagtc agcggggccg
     5821 atcatggcga tcaatgccgg ttctatccgt gcgttgtcgc acataatcat taaacaaggc
     5881 cggtgtttca gtgctgtcgg atgagggggc aggtatgcgg acgtgaggtg gcggcgacgg
     5941 ccggtctagt agtcaaagat acggatcgaa ttgcccccgg cctctttggc ccaatacatc
     6001 gcggtatcgg cccatttgat gatctcttcc gtgccgacat cgtgatcaag gaacagcacc
     6061 atgccgatgc tcgaggtgca aagatgcccg atagactttt ccgttttgtc gccaagctgc
     6121 actttcagtt cgtaggggtt ccccagcgcg acgcgaattt tttcggcaac aaaattggat
     6181 tgcctggtgg aggcggtcat atccatgtcc agttcgccga gtatcaccac aaattcgtcg
     6241 ccgccgaaac gcgcgacaaa atccacctcc cgcacacaac tgctgatgcg tcgcgccacc
     6301 tcgatcagca gcaggtctcc cacgctgtgt ccgtgcgtgt cgttaagcgt cttgaagttg
     6361 tccagatcca gaaacatcag cgcgccataa cggccgctgc gtttgctggc ggccatcgcc
     6421 tgggccagtc tgtcggttaa caggcggcga ttgggcaggc ccgtcagttc atcgtgaaat
     6481 gccagcctga caatccgttc ttccgccagc ttgcgttcgg tgatgtcgcg tgcggcggca
     6541 aacacgccct gcagcacgcc gtcgcggttg tagaaagtgg tcgcgttgta ggaaaccacg
     6601 gtttccttgc cgtctctggc gcgcacggtc agctcatagt cgcagattct tttttcgctc
     6661 aatgcctgcc tgatcccggc atcggcgcgt tccggatcgg tgaagtaact ctttgatgca
     6721 ctcccgatca gctcttcgcg cgcacagccg gtaatggctt ccatctgctt gttgacatcg
     6781 gtgatgatgc cggtcggatc ggttgccatc agcgcatcga tattggactc gatcagcgag
     6841 cgggtataga actgctggtc gctcaggttc cgattgagct gcttctgaac ggcttcaacc
     6901 tgtttttgcg cggtgttgtc ggtaccgatc aagagatagc cgatgattgc agcttcggcg
     6961 tcgcgcagtg ccgtgaccga aacgatcgcg ggcaggcggc tgccatcctt gcggatatag
     7021 gtcagctcat aaacatcctc ggtgccgcga aatgccctga acaccaacgc atcaaatccg
     7081 agcatccccg cattgccgag tgcttgcgcg cgggccagta actcctgccg atcgaacagg
     7141 tcggccggtg tctttttgtt gatgacctcg gctgcggcat agcccagcat gagctcggca
     7201 ccggcattga atacccggat ttcgcccttg gtatccgtgg cgatgttcat aaaatgggag
     7261 ctgtttaaga ttgcgatcag cagggccttg gggttgagca atgcctcctg atgccagaca
     7321 tcgaaatggc acgccgctac gcccgcatcg gtgggggaaa tgagctggtc gtagatgact
     7381 tttagcatgg gttgtttttg cgcgagcgat ttcacggcac gacggctacg gttaaacagc
     7441 ttgtagtcgc ttgggcggga gcgttccgtg cgttatccca cattgagtgg gattggctgt
     7501 gtccagggaa ggcgggcgcg gtgcccggtt gatttcggtg tgtgccgctt tgggcgtgcc
     7561 ggatgtgcgg ggggataaag aggctatccg gtcgggaagg cggccggcgc cgcgtttttt
     7621 ttgcgattta tttggcgcac atggtctcgg tcacatcctt gcctgttccc cggtagccgg
     7681 tatagcgccc ggaggcgtcg aaagtcggct ccccgctcac catgaagtat cgccgcaccc
     7741 cgccgtggct gatccggctg aagacaaaat ccaggaaagg gcgtctggcg gcgagattct
     7801 cctccagcat tttccgttcg ctctcgaccc agctcgcatc ctgatcgtct cgggactgca
     7861 ccagcgcgtc gtcgatccat atccccagca tttccagcac cggcccggag atcttggtga
     7921 aatgtccgtc cgcgttctgc tcccagtacc agtcggatga gagttccgtc aggcggcgaa
     7981 agcgcgcctc gctttcacgc agctcggccg tccgtaactg cacggtctgt tccagctctt
     8041 cgttgtactt cttcagcttc ttgtataaca gcctgacttc cagcatgttg tggatgcgcg
     8101 tctttacctc gatcagatcg aacggcttgg cgatgaagtc cttggcgccg gcttccagcg
     8161 cgtgcagctt gtggctgggc tgggccgtga cgacgagcac aggaaggtag tcttccgctt
     8221 caatttcctt caggcattcc agcacctcaa atccatccat gccgggcatc accagatcga
     8281 gcaggatcag gtcgtagtgc tggtcgcgat gcagcgcgca gacggtatag ggatccatcg
     8341 tcgagctgac attgcgatag tccgcgtcct gcagcatttg ctccagcagc gtcacattgg
     8401 cttgctgatc gtccacgatc agaattttcg cgttcagaat ttcttgctcg cagatcattt
     8461 tattcatccg gtttgtgggt gctgtctgat ccggtttcca ggaatttcag ggaatcgtcg
     8521 agcacattta aaaattcatc gatgttgatg ggcttggtga gatagcggaa aaatccattt
     8581 tgcaatgctt tctcgatatc gccaggcatc gcattggcgc tgagcgcgac cacgggaata
     8641 tgcatcgtcg ccggatcttt gcgcagaata ttcatgatct cgatgccgct gatgccgggc
     8701 agattgatat ccatcacgat cacatccggc agataggcgc gcgcaaacgc gatgccgagg
     8761 ctggggtcgc gcgtgctcag caggcgcaga tccttgtgct ggccgatgat ctgctcgacc
     8821 agcgccaggt tggccggatt gtcttccaca tacagcaggg ttttctgctt cccataccgc
     8881 agcgcgcgcc gctccatgtt ctctctgtta tccacctggg cctgcggcac atgttcgcgg
     8941 cccagttcga tccagaactc gctgcccacc ccggggatgc tttccacgcc gattgttcct
     9001 cccatcagtt cgaccagttg cttgctgacc accagaccga tgccggagcc ctccacgctg
     9061 ccggcctctt gtccgagacg gttgaacggt tgaaataact gggcctgctg tgcggccgtc
     9121 aggcctatgc cggtgtcctt gatgccgatg cgtacccgtt ccggcatgac ggtcaatttc
     9181 acttccaccg tgccgtgctc gcggttgtat ttgatcgcat tgctgagcag attgatcaga
     9241 acctgtttca accggccggg atcggcccgg acaaagcagg gggcgtcgaa tggggggaag
     9301 gtcagctgga tgccatgcag ctcggcatcg ggctcgatca aggtccggct gtcattcatc
     9361 gcggcgttca aggacaccgc gcccagcgac aacgacagct tgccggactc gatcagagcc
     9421 agattgagca actcgttgat cagatccagc aaataccatc ccgcctcgct gatctgcttc
     9481 acccgggaag cctgaacgtc actgagcggc ggcgaaccgg ctgccagcaa ctgggaaaag
     9541 cctaggatgg agttcagcgg cgtgcgcaac tcatgactca tgcgggacag aaaatcggac
     9601 tttgccagat tggcgctctc cgccgtgcat ttcgcgcgta tcaggtgaat attgtctgtc
     9661 tggagctgtt cggtctgttc ctgtgcattc atggcgttaa tgaacagccg ttcgttcact
     9721 gcccgcagca gggtcgtgcc gacatccgat gcgtcagttg cgcgagcttc agcgtcgggt
     9781 ggagtgcccg cagcctcgat cagcagtgca tattcttccc gcatctgatc gagcagctgt
     9841 ttcttgacgg caatgtcgtg cgcaaccgtt gcatccggtt gtccggcgac gccatggtcg
     9901 gtttttctgg tcatcgcccc gggggtctcg ttaggaactg acggtgggcc ggcccgacat
     9961 gatgccgtcg tagccggaca aggcggcacc gataacgatg ccctcatcgg tgatgtcaaa
    10021 caggcgaatg tccttgtcgt gtgcgctgcc gcgaactttg acgactgaaa aggcgcgctt
    10081 caactggccg gaaatttcga tgtagcgctg cacgatgatc gcgtcggcga ggaacgcgct
    10141 gccaaaagga ctgaagcgca gatcggtgta gcggtcttcc agttccgagg tcatcaggat
    10201 ggtcacgccc atgccggtca gctcggcgat catccggtac agcgagccgc gaaaatcttc
    10261 gctgaattca ggcgccagcg ccagttcgaa tccggacagc gagtcgatca cgacccgctt
    10321 cgctttcatg cggtgaatca tttcgatcag gtcgtgcagg gtctcgtcta tcgacagatc
    10381 cagcgagcgc gtgtcgatca cgccgatctg accgctctgc accagcggat aaagcttctt
    10441 gttgccgatc agctggctgg ggctcttctc gaacgcggcg atcacgccgg tttcgccccg
    10501 gcgcacgcct tcggcgagga attcggttgc caggatggtt ttgccggagc cggacgggcc
    10561 gacgaccagc agcgaatagc ccgatggcaa gccgccgccc agcatcgcat ccagtccggg
    10621 tacgcccatc ggtacccgtt cgtcttcgcc atacgaggcg atcgccgatt cgtgcggcag
    10681 ggcatgtcgc atgatcgcgc gcgggaaaat ctgaatgccc tgatcgctga tgcggaaggt
    10741 atgcaaaccg gaggcttggc cctggccgcg catcttgacc acctgcatct tgcgcaccat
    10801 cgaattgcga tgcagattct gcgaaagcca caggatgccg tcggccacgg taaagatcgg
    10861 gctggacttc gattccggct cgaggtattc gccgatcagg aaggtggtcg cctgccagct
    10921 ggtcattttc atgccgagct gctgcacgaa gcgttgcagc tcggtctcgc cctgttcatt
    10981 ctgcttggca ctgcgcacca cggagcggaa cgagtccacg aataccaggc tgggcgcata
    11041 gtcttcgact tccttggaga tgcgggccag caccagatca aagtcgcccc gcagcaggtc
    11101 tgacgacaga ttcacgaagc ggatcgattc gttgactttg ttgaagtcga agaaggggaa
    11161 ctgctgctga tagcgcagca tcttcagcgc gggctctccg agcaccgtga agaacagcgc
    11221 gcgatggttc ggggccgcga gggcgaacat gatctggtgg gcgagggtgg tttttccgct
    11281 ccccggcgtg cccgcgatca ggttgaacga aa
//
LOCUS       DASUIV010000323         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1202160, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000323 DASUIV010000000
VERSION     DASUIV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202160"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFW59_11495"
     CDS             complement(<1..248)
                     /locus_tag="VFW59_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364883.1"
                     /translation="MRRRIARLAVGIAILFSCLPGIARAGSVPDGLRAEVVGVRGLAE
                     NDPPRAYRDAQRLQTGQPADATAREENAAPKNSGTQHP"
     gene            complement(403..1356)
                     /locus_tag="VFW59_11500"
     CDS             complement(403..1356)
                     /locus_tag="VFW59_11500"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5364884.1"
                     /translation="MQTEQQTITDAVISAIDHDQIELPTLPDVAVRIRDLIDDPNVWV
                     DHLVHALSTDPVVSVHVIKAANSAAFANGKAVDTLRSAISRLGYRMLRNVVMTVTLGK
                     LFKASHPLINHQIKALWEHSREVAAVSYVLARQHRHLNPEQAMLAGLVHDIGALPLYL
                     DAERHCSHLDPATLDVLIGTFSSQVGTRLLKSWDFSAELIGVAAGHDDLQRVNDSGLP
                     DYVDVVTLANLLVREANTPVEWELVLAAARLGYGPEECRSFLADHEEHLTAVLSMLGL
                     PALKSKAATDNAGKSSPRAAPPPIARHTESGILSGLRRFFS"
     gene            complement(1434..2501)
                     /locus_tag="VFW59_11505"
     CDS             complement(1434..2501)
                     /locus_tag="VFW59_11505"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX5364885.1"
                     /translation="MRRIRVMIVDDSAVVRQVLSAALGKDSEIEIIGTASDPVFALEK
                     MQRDWPDVITLDVEMPRMDGITFLKKLMAERPTPVVICSTLTGKGTETTMQALAAGAV
                     TVVAKPTVGLKQYLQDASEDLIHAVKAAARANVRRLVPAQNAPKLNADAILPLPVVHA
                     MVQTTDSVVAIGTSTGGTQALEAVLTRLPRVCPGMVIVQHMPEKFTEAFANRLNDLCQ
                     VEVREAKNGDRVMPGRALIAPGGRHMLLKRSGAQYHVDIMDGPPVSRHRPSVDELFRS
                     VARCAGKNALGIIMTGMGDDGARGLKEMRDAGARTIGQDEASCVVYGMPKEAMKHGAV
                     DQELPLDHIAGVISAYRGRAQ"
     gene            complement(2483..3046)
                     /locus_tag="VFW59_11510"
     CDS             complement(2483..3046)
                     /locus_tag="VFW59_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX5364886.1"
                     /translation="MNKPSHVIEIFLQPGDLYFGDRDTRIRTVLGSCVSLTFWHPKLL
                     VGGMCHYMLPNRSHDRRRTSAQALDGRYADEAIDMMTKEIDAIGVPHREYQVKLFGGG
                     NMFPGRSNVKEHVGMKNVDTARKLVIRHGFNCVAEHLGGMGHRNVIFDIWNGHVWVKH
                     AVIARHEAAPEADKKDMDRRWPCVAFG"
BASE COUNT          595 a         1037 c          885 g          601 t
ORIGIN      
        1 tcgggatgtt gcgtgcctga atttttcggg gcggcattct cctcccttgc ggtcgcatcg
       61 gcgggctggc cggtctgcaa gcgctgcgca tcccgatagg cccgaggcgg atcgttttcc
      121 gccagccccc gcacaccaac gacttcggct cgcaatccgt ccggcaccga gccggcccgg
      181 gcaatgcctg gcagacacga aaagagtatt gcgatgccaa cggccagtct ggcgatccgc
      241 ctacgcaaag cgcgatgcct gtcagttttt ttacgattga aggtcgacaa tttatggctc
      301 actacaaggt aaaattttca cgattggaat ccgtcggctc cggcatgaac acatccccgc
      361 atcacccgat cgaaacgcac gctcaatgct ggcgacagcg attcaggaaa aaaaacggcg
      421 caggccggac aggatgccgg attcggtatg ccgggcaatt gggggaggtg cggcgcgagg
      481 cgaagatttt ccggcattgt ccgttgcggc tttagatttg agcgccggga gccccagcat
      541 gctcaggacg gccgtcagat gctcctcgtg atccgccaga aaactccggc attcctccgg
      601 gccgtaaccc agacgcgcgg ccgcaagcac aagctcccat tcgaccggcg tgttggcttc
      661 ccgcaccagc agattggcga gcgtcaccac atccacataa tccggcaagc cgctatcgtt
      721 cacgcgttgc agatcgtcat gcccggcggc gaccccgatc agttcagcgg aaaaatccca
      781 gcttttcagc agtctggtgc ccacctgcga cgagaacgtg ccgatcagca cgtccagcgt
      841 ggcaggatcg aggtgcgaac aatggcgctc ggcatcaagg taaagcggca acgccccgat
      901 gtcatgcacc agcccggcca gcatggcctg ctcgggattc aggtgccggt gctgtcgcgc
      961 cagcacataa ctgaccgccg ccacttcgcg gctatgttcc cacagggctt tgatctggtg
     1021 gttgatgagc ggatgggagg ccttgaacag cttgcccagc gtgaccgtca tcaccacgtt
     1081 gcgcaacatg cggtaaccca ggcgggagat tgcgctgcgc agggtatcca ccgctttacc
     1141 gttcgcaaat gccgcgctgt tggcggcctt gatgacatgc acgctcacca ccggatcggt
     1201 ggaaagcgca tgcaccagat gatccaccca cacattcgga tcgtcgatca ggtcgcgtat
     1261 cctgacggcc acgtcgggca acgtcggcag ttcgatctgg tcgtgatcaa tggcggatat
     1321 gaccgcgtcc gtgatcgtct gttgttcagt ttgcatgatg gaatatcttc gagttctgcc
     1381 gatttaaaac aatgccgaag tcgctcttgc gaaaaccgct gccactgcga aaatcactgc
     1441 gcacgccccc ggtaagcgct gatgaccccg gcgatatgat ccagcggcag ttcctgatcc
     1501 accgcgccgt gcttcatcgc ctctttcggc atgccgtaaa cgacgcaact cgcctcgtcc
     1561 tgaccgatgg tgcgcgcacc ggcatcgcgc atctccttca ggccgcgcgc accgtcgtcg
     1621 cccatgccgg tcatgatgat gcccagcgcg ttcttgccgg cacagcgcgc caccgaacgg
     1681 aacagctcat ccacagaagg acggtggcgg ctgaccggag ggccgtccat gatgtcgacg
     1741 tgatactgcg caccgctgcg cttgagcagc atgtgccgcc cgcccggcgc gatcagcgcg
     1801 cgcccgggca tcacccggtc gccgttcttc gcctcgcgca cctcgacttg gcagaggtca
     1861 ttcaaccggt tggcgaacgc ctcggtgaat ttctccggca tgtgctgcac gatcaccatg
     1921 cccggacaca ctcgcggcaa gcgggtcaac acggcctcca gcgcctgcgt gccgccggtc
     1981 gaggtgccga tcgccaccac cgagtccgtc gtctgcacca tcgcgtgtac cacaggcagc
     2041 ggcaggatcg catcggcgtt gagtttcggc gcattctgcg caggcaccag ccgacgcacg
     2101 ttggcccgcg ccgccgcctt gaccgcatgg atcaggtctt ccgaggcatc ctgcaaatac
     2161 tgtttcaacc cgacggtagg cttggccacc accgtcaccg ccccggcggc cagtgcctgc
     2221 atggtcgtct cggtgccctt gccggtcagc gtcgaacaga tcaccaccgg ggtcggacgc
     2281 tcggccatca gcttcttcag gaaggtgatg ccatccatgc gcggcatttc cacatcaagc
     2341 gtgatcacgt ccggccagtc gcgctgcatc ttctccagcg cgaataccgg atcggaagcg
     2401 gtgccgatta tttcgatctc gctatccttc cccagcgcgg cagacaacac ctgacgcacc
     2461 acggccgaat cgtccacgat catcacccga atgcgacgca cggccatctc ctgtccatat
     2521 cctttttatc cgcttccggc gcagcctcat gccgcgcgat gaccgcatgc ttgacccaga
     2581 catgaccgtt ccagatatcg aagatcacat tgcggtgccc catcccgccg agatgctcgg
     2641 ccacacaatt gaagccgtgc cggataacca gtttgcgtgc ggtatccacg ttcttcatcc
     2701 ccacatgctc ctttacgttg ctccgccccg gaaacatgtt gccgccgccg aacagcttga
     2761 cctgatactc gcggtgcggc acgccgatgg catcgatctc tttggtcatc atatcgatcg
     2821 cctcgtcggc atagcgcccg tccagtgcct gcgcgctggt acggcgccgg tcgtgcgaac
     2881 ggttcggcag catgtagtgg cacatcccgc cgaccagcag tttcggatgc cagaacgtca
     2941 gcgacacgca ggatcccagc acggtgcgga tacgcgtatc ccggtcgccg aaatacaggt
     3001 cgcccggctg caaaaatatc tcgatgacgt gcgacggttt attcatatca ggatccctgg
     3061 taatccgtca ttcccgcgcg ggaatgacgg aatgagtgca gctcacagct tccggtac
//
LOCUS       DASUIV010000324        14268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_178716, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000324 DASUIV010000000
VERSION     DASUIV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 14268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14268
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178716"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VFW59_11515"
     CDS             <1..1187
                     /locus_tag="VFW59_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX5364887.1"
                     /translation="NVEKQIELVRADESLDEAAKEQRIAQLKSEWQQLHQQVLDSGGL
                     HIIGTERHESRRVDNQLRGRSGRQGDPGSSRFFLSLEDPLLRIFASDRVAAIMNRFKL
                     PEGEAIEHSWVTRAIENAQRKVEARNFDMRKQILEYDDVANDQRKVIYQQRSELLEST
                     DISETIAAMRDGVMDATISQYIAPGSMEEQWDVPALEKMLASEFRLELPLVQWLDQEK
                     QLDEAGLRARIAEHAREQYQAKVDLVGPEMHHYERMIMLQSLDQHWREHLAALDHLRQ
                     GIHLRGYAQKNPKQEYKREAFELFSTMLEAIKHEVTQVLMTVQVRDQSDVESVEAIPT
                     PENVQYHHADYDEALGHASESESTDEGAGTFVRDGQKVGRNDPCPCGSGKKYKQCHGK
                     LN"
     gene            1209..1694
                     /locus_tag="VFW59_11520"
     CDS             1209..1694
                     /locus_tag="VFW59_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5364888.1"
                     /translation="MSLVIDTLRTSSITEELSDAEVEILAGLFEVRDYKNGDVIVEPG
                     DDQPDNLYILAHGDIEVKIQSAEGQSTIHVLKPGDLAGMITFVGGAATQVSATLYAVG
                     NTKVLGLERSKFETLINSHPMIVYRVMRGLARNMHGIVRRMNTESAELSNYIYRTHGR
                     Y"
     gene            1707..2924
                     /gene="argJ"
                     /locus_tag="VFW59_11525"
     CDS             1707..2924
                     /gene="argJ"
                     /locus_tag="VFW59_11525"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX5364889.1"
                     /translation="MPVNLTTPVAADLLPVAGVSLGAAEAGIKRPDRKDLLVMQLCEG
                     AQVAGVFTTNRFCAAPVIVAREHLAQGEGIRALVVNTGNANAGTGAQGMQDARDTCAA
                     LAGLLGCKAAQILPFSTGVIMEPLPVAKIAAGLPAAVANMVENNWFDAAHAIMTTDIV
                     AKALSRQIEIDGKTVTVTGMSKGSGMIHPNMATMLGYIATDAAVAQPLLQEIVSEAAN
                     RSFNCITVDGDTSTNDALMLIATGKSGAVIDQANRARLQAVVTEVATALAQAIVRDGE
                     GATKFITLRIEGGKDEAECKKIGYAIAHSPLVKTAFFASDPNLGRILAAIGYAGVADL
                     DVEKLKLYLDDVLVAENGGRAASYQEADGQRVMQQSDITIRVVLNRGAVNATLWSCDF
                     SYDYVKINASYRS"
     gene            2945..3799
                     /locus_tag="VFW59_11530"
     CDS             2945..3799
                     /locus_tag="VFW59_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364890.1"
                     /translation="MDKLDHFLNRAEGLLARLEQVLPGAPVPAPDWRAAAAFRWDQRQ
                     RMLHPVPNFQRIALADLLGIDDQKQRIRQNTQQFVNGGTANNVLLSGARGTGKSSLVK
                     ALLNEYAEQGLRVIQIDKQGLVDLPHIVGLVQGRDERFILYCDDLSFNADEPGYQSLK
                     AALDGDLVAFSDNLLIYATSNRRHLMPDYMQDNLATRHVGDEVHPAESVEEKVSLSER
                     FGLWVSFYPFSQDEYLAVAAHWLAHHGWKQGMTDEVRLAALQWSLMRGSRSGRVAGQF
                     AKDWCGRN"
     gene            complement(3847..5070)
                     /locus_tag="VFW59_11535"
     CDS             complement(3847..5070)
                     /locus_tag="VFW59_11535"
                     /inference="COORDINATES: protein motif:HMM:NF023301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5364891.1"
                     /translation="MLSSLAALLLLTSCSSNSTRSLVNIATSGTARTVVQIAASGSPE
                     AAARALIKRKELAYARNPLQAANDLQAVQRDFRRLFDLLSGKVSKKWGKDDTRLPDRT
                     HYVKYTQGYLSRAVVDFDTGEITVETLDDKNTERSLKSAIVTTLLTPDDPRAVDLFTD
                     QSIRLSSERDPYLLGLVLDDSGRAIATPKQAERFADGMIGIALTRKVKTDAGEKQARF
                     VKFDMVNNYTNKQAEKYRPQVEKFARTYNISPSLVFAIMRTESNFNPFAVSSAPAYGL
                     MQLVPSSGGRDAYRRVKGSDTIPSKQYLFDADNNIELGTAYLSVLTFNELDGVTDPVS
                     REYCVISAYNTGSGNVMRAFGGSSRNRDAALNDINRLPAPAVYSQLRERLPYAETRQY
                     LQKVVGFRKQFVSVN"
     gene            complement(5090..7858)
                     /gene="ppc"
                     /locus_tag="VFW59_11540"
     CDS             complement(5090..7858)
                     /gene="ppc"
                     /locus_tag="VFW59_11540"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX5364892.1"
                     /translation="MKLFSAPADISSKDLPLREDVRLLGSILGDTLREQEGEASYQLI
                     ENVRRAAVRFRKTQDDRDREQLEQTLDALSPSETLVVVRAFSYFSQLSNIAEDLHHNR
                     RHRAHLKAGSTPKDGTLQRALNRLEEKKIDSKTLQSFLNSALVSPVLTAHPTEVQRKS
                     TLDCQLIISSLLSQRDRMDMTPDDEADNEEALRRFVLILWQTRMLRTSKLTVRDEISN
                     GLEYYRYTFLNEIPKIYAGLEKHLEARFDKDIKVPSLLRVGSWIGGDRDGNPFVTHDV
                     MSDAVRQHSALAFEHYLNETYVLGRRLSLTDRVVEITPELRKLSDASPDKDTARTDEP
                     YRRALNLIYSRLSSTARKLGHTLTHLPPLDPDAQPYATHQEFIADLDILVDSLFKHGA
                     VYLARGRLANLRRAAEVFGFYLAPLDMRQHSAIIEQTVSELYSHSSGKANYIDLDEAT
                     KRSVLLETLQAGKILLTDIERYSAVPQSELRIMQAAADIHQRFGRAALPNHIISKTDA
                     VSDMLELALVLQQVGLLEGSDKLHVNIIPLFETIEDLRGGARIMDELFSIPWYRKLLE
                     SRSDTQEVMLGYSDSNKDGGYLTANWELYKAELELVQVFEKHGIELRLFHGRGGTVGR
                     GGGPSYDAILAQPPGSVNGQIRITEQGEVISSKYSNPEIGRRNLETLIAATMEATLLH
                     HHGADSAMPEFHRIMEALSLDAFAAYRKLVYETPGFTEYFFTATPIREIAELNIGSRP
                     SSRKASDKIEDLRAIPWVFSWGLNRVMLPGWLGFGSAVRQFIEREGDAGLAQLQLMYK
                     NWAFFRGLMSNMDMVLSKTDMGIASRYADLVEDVELRERIFGAIEQEWKITIEMLFAV
                     TGNTTLLQDNPAFARSLLTRSPYIDPLNHLQVALLHRHRAGDNDELVKRAIHLTINGI
                     ATGLRNSG"
     gene            7938..8897
                     /gene="hemC"
                     /locus_tag="VFW59_11545"
     CDS             7938..8897
                     /gene="hemC"
                     /locus_tag="VFW59_11545"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX5364893.1"
                     /translation="MSQASPTSPSPTSPSRLVIASRESALAMWQAEHIRDRLRALYPQ
                     TEVSILGMTTQGDQILDVTLSKIGGKGLFVKELETALEDGRADLAVHSLKDVPMHLPE
                     GFVLAAIGEREDPHDAFVSNNYENLAALPAGSVVGTSSLRRESQLRARFPHLKIEPLR
                     GNVQTRLRKLDEGLYAAIILAAAGLKRLGLGGRIRGIIASEDSLPAVGQGALGIECRA
                     DRADVIAALQPLHHADTAACVLAERAMSRALAGSCQVPLGGFAEVQGDKLRMRGFVAS
                     PDGQRMLRAEAIGDIADPEALGSQIADTLLAQGAGEILAALNG"
     gene            8890..9660
                     /locus_tag="VFW59_11550"
     CDS             8890..9660
                     /locus_tag="VFW59_11550"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5364894.1"
                     /translation="MVELPLRGLTVAVTRPRDQAAQLAQRIAQAGGVPLLFPLLDIAA
                     MPDDGVLSEQLSRLAQFNLAVFISPNAVQYGMAAIRRAGGLPAQLGIATVGQGSAKAL
                     RELGVEEVIAPTERFDSEGLLALPQLQHVAGWRVLIFRGDGGRELLGDTLKARGATVE
                     YAACYRRGKPHQDVGALLSAAPDAITVTSSEALTYLQQMLEAEEGLGWRDIPLFVPHA
                     RIAELAGRQGWQRVVTTGAGDDGLLAGLLAWAQKEESR"
     gene            9657..10772
                     /locus_tag="VFW59_11555"
     CDS             9657..10772
                     /locus_tag="VFW59_11555"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5364895.1"
                     /translation="MNEPTPAQETIAPISVAPDGAISPPRQRTSVADLFARISVTQMT
                     LAVLVAIFLWQWFAGHLAISDMRREIAEKIAEMDGTSKANQIMLERSQDQVRELSAKV
                     ATLESHYAESQNQRAALETLYNDLSISRDETALAEVEQMLLIAGQQLQLSANVKAALI
                     AMQSADARLQRMDRPAFNGLRKAIGQDMVKLRALPNVDVAGINDQLNELMGGVDGLPL
                     IYQQRVARTAHEQPAAPNETAWQGLVREIWQDIRQLVRIENTGKVEIPLLPPEQEFFL
                     RENLKMRFQAARLALLSRDEVAFRQELKTAQFWTVRYFDTGAGAGMKMLAGVKRLASA
                     SINIELPDISPSLQAVRSYRLTRDGAGDRGLQSKAAR"
     gene            10769..11959
                     /locus_tag="VFW59_11560"
     CDS             10769..11959
                     /locus_tag="VFW59_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEX5364896.1"
                     /translation="MKYLLWILLLFAAAVALTTASHNPAYVLLVYPPYRIELSMMLFA
                     VLLLLAFIFSYGLVRLLLAAVQLPAYVKKFRLERAQARARELLDETLTAFFEGRYAAA
                     EKAAARAMELGDTSSLHPIIAARSAHELREYEKRDAYLTAVEGKSAGDSTMRLLATTK
                     FMLDQHDPRAALNTLQQLRASGVKSHVGALSLELKAQQQIGNWDGVLQTLDQLEKRNA
                     IDVTVAAQLRQQAWLEKIRQQEDLAGLTACLKELPGELKRRSKVAATAARELIRHGDC
                     PLAQQLLADSLNAQWDSELVALYGDCHSDGAAADDVIPKIEQAEKWLNQHKRDAGLLL
                     ALGKLCLHQGLWGKARNYLDASVSIAPTGAAYTALAQLAARLGEAAEASKYYQQAAGL
                     RSED"
     gene            complement(11981..12994)
                     /locus_tag="VFW59_11565"
     CDS             complement(11981..12994)
                     /locus_tag="VFW59_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEX5364897.1"
                     /translation="MTFNTTILPSNHRFSVEADETILEASLKHGCNLPYSCQNGACGV
                     CKGKVIEGEVDYGNYQPHALSDAEKEAGMALFCCAKPLTDLIIESREITATKDIQVKT
                     MPCRIDKMEKVAPDVMVLKLKLPANERLQFLAGQYIDILQKDQKPRSFSLANAPHHDD
                     LLELHIRNIAGGSFTQHVFSDMKERDILRLKGPLGSFFLREDSDKPIIFVASGTGFAP
                     IKAIIEHALYIGVKRPMHFYWGARTLADLYMLETARLWESNGIKFTPVLSEPTATDGW
                     QGRTGFVHQAVLDDYQDLSGYETYVCGAPVVVEAAHRDFTSQRGLPDDAFFSDAFTFA
                     PKG"
     gene            13046..13951
                     /locus_tag="VFW59_11570"
     CDS             13046..13951
                     /locus_tag="VFW59_11570"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5364898.1"
                     /translation="MKRLLIVGCGDIALRILPLLLPHYRVYALLRNQARHAELRARGA
                     IPLAGDLDDRASLARIAGLAEAVLHLAPPANTGTVDARTRNLLAALSLAKPPRRFIYI
                     STSGVYGDCAGAGVSETRPVNPQNSRAQRRVDAEQRIREWAARNRVRASILRVPGIYA
                     ADRLPLERLRANTPAIVTAEDGYTNHIHADDLARIAIAALHRGKANRVYHASDDSELK
                     MGDYFDAVADAHGLPRPPRLSRAEVERSVSPMLWSFMNESRRLTNQRMKVELQVALRY
                     PTVVDALEGRVNRAGKERMPLIFID"
     gene            14044..14169
                     /locus_tag="VFW59_11575"
                     /pseudo
     CDS             14044..14169
                     /locus_tag="VFW59_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilE"
BASE COUNT         2726 a         4035 c         4671 g         2836 t
ORIGIN      
        1 gcaatgtcga gaaacaaatc gaactggtcc gcgcggatga aagtctggac gaagccgcaa
       61 aagagcagcg tatcgcgcaa ttgaaatccg aatggcagca actgcaccag caagtgctgg
      121 acagcggcgg gctgcacatc atcggcaccg agcgccacga atcgcgccgc gtggacaatc
      181 agttgcgcgg ccgttcggga cgccagggcg accccggttc cagccgtttt ttcctgtcgc
      241 tggaggatcc gctgctgcgc atcttcgctt ccgaccgtgt cgcggcgatc atgaaccgtt
      301 tcaagctgcc cgagggcgag gccatcgagc acagctgggt gacgcgtgcc atcgagaatg
      361 cgcagcgcaa ggtcgaggcg cgcaacttcg acatgcgcaa gcagatcctc gaatacgacg
      421 atgtcgccaa cgaccagcgc aaggtgatct accagcagcg cagcgagctg ctggaaagta
      481 ccgatatttc cgagaccatc gctgcgatgc gcgatggggt gatggatgcg acgatcagcc
      541 aatacatcgc gccgggcagt atggaagagc aatgggatgt gcctgcgctg gaaaaaatgc
      601 tggcaagcga gtttcgtctg gagttgccgc tggtgcagtg gctggatcag gagaagcaac
      661 tggatgaggc tggcttgcgt gcgcgcattg ccgagcatgc acgggagcag tatcaggcca
      721 aggtcgatct ggtcgggccg gagatgcatc attacgagcg catgatcatg ctgcagagcc
      781 tggatcagca ttggcgcgaa catctggcgg cgctggatca tctgcgtcag ggtatccacc
      841 tgcgcggtta cgcgcagaag aacccgaagc aggaatacaa acgggaggcc ttcgaactgt
      901 tctcaaccat gctggaggcg atcaagcatg aggtgactca ggtgctgatg acggttcagg
      961 ttcgagatca atccgacgtc gagtcggtag aagctatccc aactccggaa aatgtgcagt
     1021 atcatcacgc cgactacgac gaagcgctgg gtcatgccag tgaaagtgag tcaaccgatg
     1081 agggtgcagg gacgttcgtg cgcgatggcc agaaggttgg acgcaatgat ccttgcccat
     1141 gtggttccgg taaaaaatat aagcagtgtc acggcaaact gaattaatac gcaaagggga
     1201 gaatgtaaat gtcactagtg attgatacgc tgcgcacgtc aagcatcacc gaggagctga
     1261 gcgacgccga ggtggagatt ctggcgggtc tgttcgaagt acgcgattac aaaaacggtg
     1321 atgtcatcgt tgagcccggc gatgaccagc cggacaatct gtacattctg gcgcacggcg
     1381 atatcgaagt gaagatacag agtgcggaag ggcagtctac catccacgtg ctcaagcccg
     1441 gcgatctggc tggcatgatc actttcgtag gtggtgccgc cacgcaggtc agcgcgacgc
     1501 tgtatgcagt aggcaacacc aaggtgttgg gtctggagcg ctccaagttc gaaacgctga
     1561 tcaacagtca tccgatgatc gtgtaccgcg tgatgcgcgg attggcgcgc aacatgcatg
     1621 gcatcgtgcg ccgcatgaac accgagtctg ccgaactcag caactacatc tatcgtaccc
     1681 acggccgtta ctaaagaaag agctttatgc cggtcaatct gactacgccc gtcgcggccg
     1741 acctgttgcc cgttgcgggc gtttctctgg gtgccgcaga ggcgggtatc aaacgccccg
     1801 atcgcaaaga cctgctggtg atgcagctgt gcgagggcgc acaggtcgcc ggcgtgttca
     1861 ccaccaatcg attctgtgct gcgccggtca tcgtcgcgcg cgagcatctg gcgcagggtg
     1921 aggggatacg cgcgctggtg gtcaacaccg gcaatgccaa cgcgggtacc ggtgcgcagg
     1981 gtatgcagga tgcgcgcgac acctgtgctg cgctggccgg cctgcttggc tgcaaggccg
     2041 cgcagattct gccgttctcc acgggcgtga tcatggagcc tttgccggtg gcgaagatcg
     2101 ccgccgggtt gcctgccgcc gtggcgaata tggttgagaa caactggttc gacgccgccc
     2161 atgccatcat gacgaccgac atcgtcgcca aggccttgtc gcgccagata gagatcgacg
     2221 gcaagacggt caccgtgacc ggtatgtcca agggctccgg catgatccat cccaatatgg
     2281 ccaccatgct gggctatatc gcgaccgatg ccgccgtggc gcagccgctg ctgcaggaaa
     2341 tcgtgagcga agcggccaac cgttcgttca actgcatcac cgtggacggc gatacctcga
     2401 ccaacgacgc attgatgctg attgccaccg gcaagagcgg cgcggtgatt gatcaggcga
     2461 accgcgcccg attgcaggcg gtggtcactg aagttgccac cgcgcttgcg caagccatcg
     2521 tgcgtgacgg cgaaggtgcg accaagttca tcaccctgcg gatcgaaggc ggcaaagacg
     2581 aggccgagtg caagaagatc ggttatgcca tcgcccactc accgctggtg aagacggcat
     2641 tcttcgcttc cgaccccaac ctcgggcgca tcctggcagc catcggctat gccggtgtgg
     2701 ctgacctcga tgtcgagaag ctgaagctgt atctggacga tgtgctggtg gccgaaaacg
     2761 gcgggcgtgc ggcgagctat caggaagccg acggccagcg cgtgatgcag cagagcgaca
     2821 tcactatccg cgtggtgctg aatcgcggtg cggtgaacgc cacgctgtgg agttgcgatt
     2881 tctcctacga ttacgtgaag atcaacgcca gctacagaag ctaaaaccta aaaggttttg
     2941 cttaatggac aaactcgacc acttcctcaa tcgcgccgaa ggcttgctcg cacggctgga
     3001 acaagtattg cccggcgcgc ccgtgccagc gccggattgg cgggcggctg cggcatttcg
     3061 ctgggatcag cggcaacgca tgctgcatcc cgtgccgaat ttccagcgca tcgcgctcgc
     3121 cgacctgctc ggtatcgacg accagaaaca gcgcatccgg cagaacacgc agcagttcgt
     3181 gaatggcggg acggccaaca acgtgctgct ctccggcgca cgcggcacgg gcaaatcgtc
     3241 gctggtcaag gcattgctca acgaatacgc cgaacaaggc ttgcgtgtga ttcagatcga
     3301 caagcagggc ttggtcgatc tgccgcatat cgtcgggctg gtgcaggggc gtgacgagcg
     3361 tttcatcctg tactgcgacg acctctcctt caatgccgac gagccgggct accagtcgct
     3421 caaggccgcg ctggacggcg atctggtggc gttctcggac aacctgctga tctacgccac
     3481 atcgaaccgc cgtcacctga tgccggacta catgcaggac aacctcgcca ccagacatgt
     3541 tggcgacgaa gtgcatccgg cggaaagcgt ggaggagaag gtttcgctgt cggagcgatt
     3601 cggtttgtgg gtgtcgttct atcccttcag tcaggacgag tatctggcgg tggccgcgca
     3661 ctggctggcg caccacggct ggaagcaggg catgacggac gaggtgcgtc tggcggcgtt
     3721 gcaatggtcg ctgatgagag gttcgcggag cgggcgcgtg gccgggcagt tcgcgaagga
     3781 ctggtgcggg cgaaactgag cttgccggcg gtcctgagcc ggcggagccg tatcgaagga
     3841 cagcccttag ttcaccgaaa caaactgctt gcggaatccc actacctttt gcaaatactg
     3901 gcgcgtctcc gcatagggca ggcgttcgcg caactggctg tagacggcag gcgcgggcag
     3961 gcggttgatg tcgttaagcg ccgcatcgcg gtttctggat gagccgccga aggcgcgcat
     4021 cacattgccg gagccggtgt tgtaggcgga gatgacacag tattcccgcg acacagggtc
     4081 ggtcacgccg tcgagttcgt tgaacgtcag cacgctcagg taggccgtgc cgagctcgat
     4141 gttgttgtcg gcatcgaaca gatattgctt gctgggaatg gtgtccgagc ccttgacgcg
     4201 ccggtaggca tcgcgcccgc cgctgctggg gaccagttgc atcagaccgt aggcgggggc
     4261 ggaactgact gcgaacgggt tgaaattgct ttcggtgcgc atgatggcga acaccaggct
     4321 ggggctgatg ttgtaggtgc gggcgaattt ctctacctgc gggcggtatt tttcggcctg
     4381 cttgttggtg taattgttga ccatgtcgaa cttgacgaag cgagcctgtt tctcgccggc
     4441 atccgtcttt accttgcgag tgagcgctat gccaatcatg ccgtcggcga agcgctcggc
     4501 ctgtttcggg gtggcgatgg cgcgcccgct atcgtccagt accagaccca ataaataggg
     4561 gtcgcgttcg ctggacaggc ggatggattg gtcggtgaac agatcaaccg cccggggatc
     4621 atccggtgtg agcagcgtgg tcacgatagc gctcttcagg ctgcgctcgg tgttcttgtc
     4681 gtccagtgtc tcgaccgtga tttcaccggt gtcgaaatcc acgacggcgc gcgagagata
     4741 gccctgtgtg tacttcacgt aatgggtgcg gtcaggtaag cgggtatcgt ccttgcccca
     4801 tttcttgctc accttgccgc tcaacaagtc gaacaggcgc cggaaatcgc gttgcaccgc
     4861 ttggaggtcg ttggcggcct gaagcggatt gcgcgcatag gcgagctcct tgcgtttgat
     4921 cagtgcgcgt gcggcggctt cagggctgcc gctggcggcg atctggacga cggtgcgtgc
     4981 cgtgccgctg gtggcgatgt tgaccaggct gcgggtagag ttgctgctgc acgaggtgag
     5041 cagcaggaga gcagcgagcg atgacagcat gctgcgtccc atgttccgtc tagccactgt
     5101 tgcgcaatcc ggtggcgatg ccgttgatgg tcagatggat cgcgcgtttc accagttcgt
     5161 cattgtcacc ggcgcggtga cggtgcagca gtgcgacctg caggtggttc agcgggtcga
     5221 tgtacggcga acgggtcaac aggctgcgtg cgaatgccgg gttgtcctgc agcagtgtcg
     5281 tgttgccggt gacggcaaac agcatctcga tcgtgatttt ccattcctgt tcgatcgcgc
     5341 cgaagatgcg ctcgcgcagc tccacgtcct ctaccagatc cgcatagcgc gaggcgatgc
     5401 ccatgtcggt cttcgacagc accatgtcca tgttggacat caggccgcgg aagaacgccc
     5461 agttcttgta catcaattgc aactgcgcaa gtccggcgtc gccttcgcgc tcgatgaact
     5521 gccgcaccgc gctgccgaat ccgagccagc ccggcagcat cacgcggttc aggccccagc
     5581 tgaataccca ggggatcgcg cgcaggtctt cgatcttgtc ggaggccttg cgcgacgacg
     5641 gacggctgcc gatgttgagt tcggcgattt cgcggatcgg tgtcgcggtg aagaagtatt
     5701 cggtgaagcc cggggtttca tacaccagtt tccgataggc ggcgaatgca tccaggctca
     5761 gtgcttccat gatgcggtga aattccggca tcgcgctgtc cgcgccgtga tggtgcagca
     5821 gcgtggcttc catggtcgct gcgatcagcg tctccagatt gcggcggccg atctccggat
     5881 tggagtattt gctggagatg acctcgccct gttcggtgat gcggatctgg ccgttcacgc
     5941 tccctggcgg ctgtgccaga atcgcatcgt agctggggcc gccgccacgg ccaacggtgc
     6001 cgccacggcc gtggaacagg cgcagctcga tgccgtgttt ctcgaacacc tgcaccagtt
     6061 ccagctcggc cttgtacagc tcccagttcg cggtcagata gccgccgtcc ttgttgctgt
     6121 cggaatagcc cagcatcact tcctgggtgt cgctgcggct ttccagcagc ttgcggtacc
     6181 aggggatgga gaacagctcg tccatgatcc tggcgccgcc gcgcagatcc tcgatggtct
     6241 cgaacagcgg gatgatgttg acatgcagct tgtcgctgcc ttccagcagg ccgacctgtt
     6301 gcagcaccag tgcgagctcc agcatgtcgc tgaccgcatc ggtcttggag atgatgtggt
     6361 tcggcaatgc ggcgcggccg aagcgctgat ggatgtcggc tgcggcctgc atgatgcgca
     6421 gctcgctctg cggtacggcg gaatagcgct cgatgtcggt cagcaagatt tttcctgcct
     6481 gcagcgtctc cagcagcacg ctgcgcttcg tcgcttcgtc gaggtcaatg tagttagcct
     6541 tgccggagct gtgcgagtaa agctcggata cggtctgttc gatgatcgca ctgtgctggc
     6601 gcatgtccag cggcgcgagg tagaagccga acacttccgc tgcgcggcgc aggttggcca
     6661 ggcggccgcg tgccagataa accgcgccgt gtttgaacag cgagtcgacc agaatatcca
     6721 gatcggcgat gaattcctga tgtgtggcat agggctgcgc atccggatcc agcggcggca
     6781 ggtgagtcag ggtgtggccg agcttgcggg cggtggatga caggcgcgaa tagataaggt
     6841 tgagcgcacg gcgatagggt tcatcggtgc gcgcggtgtc cttgtccggc gaggcgtcgg
     6901 agagcttgcg caactcgggc gtgatctcca ccacccggtc ggtcaggctc agacgacggc
     6961 ccagcacata ggtctcgttc aaatagtgct cgaatgccag cgccgaatgc tggcgcaccg
     7021 catccgacat cacgtcatgg gtgacgaacg ggttgccgtc gcggtcgccg ccgatccagc
     7081 tgcccacgcg taacagcgat ggcaccttga tatccttgtc gaagcgcgct tccagatgtt
     7141 tttccagccc ggcatagatc ttcgggatct cgttcaggaa ggtatagcgg taatactcca
     7201 gtccgttgct gatctcgtcg cgcacggtca gcttggacgt gcgcagcatg cgtgtctgcc
     7261 acaggatcag cacgaagcgg cgcagcgcct cctcgttgtc ggcttcgtcg tccggcgtca
     7321 tgtccatgcg gtcgcgctgg gacagcaggc tggagatgat cagctggcaa tcgagggtgc
     7381 tcttgcgttg cacctcggtc ggatgggcgg tcagcaccgg cgacaccagt gcgctgttca
     7441 ggaaggattg cagcgtcttg ctgtcgatct ttttctcttc gaggcggttc agcgcccgtt
     7501 gcagcgtgcc gtccttgggc gtgctgcccg ctttcaggtg ggcgcggtgg cggcgattgt
     7561 gatgcaggtc ttcggcgatg ttggacagct gcgagaaata gctgaacgcg cgcaccacca
     7621 ccaaggtctc gctggggctc agcgcatcca gcgtctgttc gagctgctcg cgatcccggt
     7681 cgtcctgagt cttgcggaag cgcactgcgg cgcgacgcac gttctcgatc agctgatagg
     7741 aggcttcgcc ttcctgctcg cgcagggtgt cgccgagaat gctgccgagc aggcgcacgt
     7801 cttcgcgtag tggcaaatct ttactggaaa tatcggcagg ggcggaaaat aatttcatca
     7861 cattcatacg caaaaaaacg cggcacgcct catgttagaa tgcgccgcgc attaactaca
     7921 aaatttcagg aaatccgatg agccaagcat ccccgacttc accgtcgcca acttctcctt
     7981 cccgtttagt gatcgcttcg cgcgaaagcg cgctagccat gtggcaagcc gaacatatcc
     8041 gggacagact gcgcgcatta tatccgcaaa ccgaggtcag catattgggc atgaccacgc
     8101 agggcgatca gattctggat gtcacgttgt ccaagatcgg cggcaagggc ttgtttgtga
     8161 aggagctgga gactgcgctg gaggacggtc gtgcggatct ggcggtgcat tcgctcaagg
     8221 atgtgccgat gcatctgccg gaaggttttg tgctggccgc gatcggcgag cgcgaagacc
     8281 cgcacgatgc cttcgtgtcg aacaactatg aaaatctcgc cgcgctgccc gcaggcagcg
     8341 tggtcggcac ttccagtctg cgccgcgaaa gccagttgcg ggcacgcttc cctcacctga
     8401 agatcgagcc gctgcgcggc aacgtgcaga cacggttgcg caagctggac gaaggcttgt
     8461 atgccgcgat catcctcgcc gccgccggcc tgaaacgcct ggggctgggc ggccgcattc
     8521 gcggcatcat cgcaagcgag gacagcctgc ctgcggtcgg gcagggggcg ttgggtatcg
     8581 aatgccgcgc cgaccgcgcc gatgtgatcg ccgcattgca gccgctgcac catgccgata
     8641 ctgccgcctg tgtgctggcg gagcgcgcca tgagccgggc gctggcgggt agctgtcagg
     8701 ttcctctggg cggtttcgcc gaagtgcagg gcgacaagct gcgcatgcgc ggtttcgtcg
     8761 ccagtccgga tggtcagcgc atgctgcgcg cagaagccat cggcgacata gccgacccgg
     8821 aagcattggg cagccagatc gccgatacct tgctggcaca gggcgcgggc gaaatcctcg
     8881 ccgcactgaa tggttgaatt gcctctgcgt ggtttgactg tcgcggtcac caggccgcgt
     8941 gatcaggctg cgcaattggc gcaacgcatc gcacaggcag gcggagtgcc gttgctgttt
     9001 ccgctgctcg acattgcggc catgcccgat gacggcgtgt tgagcgagca actctcccgc
     9061 cttgcgcaat tcaatcttgc tgtttttatc agtccgaacg cggtgcaata tggcatggcg
     9121 gcgatccgcc gggctggcgg gctgcctgct caactcggca tcgctacggt cggtcagggc
     9181 agcgcgaagg cattgcgcga attgggtgtt gaagaggtca tcgcacccac cgagcgtttc
     9241 gatagcgagg gcttgctggc gctcccgcaa ttgcagcatg ttgcgggctg gcgagtgttg
     9301 atttttcgcg gcgatggcgg acgcgagctg ctgggtgata cgctcaaggc gcgcggggca
     9361 acggtggaat acgccgcctg ctatcggcgc ggtaagccgc atcaggatgt cggtgcgttg
     9421 ttgagcgccg ctcccgatgc gatcacggtg accagcagcg aggcgctgac ctatctgcag
     9481 cagatgctgg aggctgaaga aggtttgggc tggcgcgata ttcccttgtt cgtgccgcat
     9541 gcccgtatcg ccgagctggc cggtcggcaa ggctggcaac gggtggtgac gaccggggcg
     9601 ggagatgacg gattgctggc cggtttgcta gcatgggcac aaaaggaaga gtcaagatga
     9661 acgagccaac acccgcgcag gaaacgatcg ctccgattag tgtcgcaccg gacggcgcca
     9721 tcagtccgcc tcgccagcgc acctcggtgg cggatttgtt cgcacgtatc agcgtcacgc
     9781 agatgacgct ggcggtgctg gtcgcgatct tcttgtggca atggtttgcg gggcacctcg
     9841 ccatcagcga tatgcgcagg gagatagcgg agaagatcgc cgagatggat ggcacgagca
     9901 aggctaacca gatcatgctg gagcgcagtc aggatcaggt gcgcgagttg agcgccaagg
     9961 tggcgacgct ggagtcgcat tacgccgaat cgcagaatca acgcgccgcg ctggaaaccc
    10021 tatacaacga tctttcgatc agccgggatg aaaccgcgct ggccgaggtc gagcagatgt
    10081 tgctgatcgc cggtcagcag ttgcaattgt cggccaatgt gaaggcggcc ttgattgcga
    10141 tgcaaagtgc cgatgcgcga ctgcagcgca tggacagacc cgcgttcaat ggtctgcgca
    10201 aggccatcgg gcaggatatg gtgaagttgc gcgccttgcc caatgtggat gtggccggca
    10261 tcaacgatca gctcaatgaa ctgatgggcg gggtagacgg cttgccgctg atctatcagc
    10321 agcgtgttgc caggacggcg cacgagcagc ccgccgctcc caatgaaact gcctggcagg
    10381 gtctggtgcg cgagatatgg caggacatca ggcagctggt gcgtatcgag aacaccggaa
    10441 aggtcgagat acccttgctg ccgccagagc aggaattctt cctgcgcgag aatctcaaga
    10501 tgcgtttcca ggcggcgcgt ctggcgttgc tgtcgcgcga tgaggtcgcc ttcaggcagg
    10561 agttgaagac ggcgcagttc tggacggtac gctatttcga taccggggca ggcgcgggca
    10621 tgaaaatgct ggcaggcgtg aagaggctcg cgagcgcgag catcaacatc gaattgccgg
    10681 atatttcgcc cagcctgcag gcggtgcgca gttatcgttt gacccgcgat ggcgcggggg
    10741 atcgaggtct tcagtcgaaa gcggcgcgat gaaatatctg ctgtggattc tgttgctgtt
    10801 cgccgctgcg gtcgccttga ccacggcctc gcataatccg gcctatgtgc tgctggtata
    10861 tccgccttat cgcatcgagt tgtcgatgat gttgttcgcc gtactgttgc tgctggcgtt
    10921 catctttagc tatggtctgg tgcgtttgct gctggccgcc gtgcagctgc cagcctatgt
    10981 gaagaaattc cggctggagc gcgctcaggc ccgtgcgcgc gaattactgg atgagacgct
    11041 cacggctttc ttcgagggtc gctacgcggc agcggaaaag gcggcggcgc gggcgatgga
    11101 gttgggcgat acctcatcgc tgcatcccat catcgcggca cgcagtgcgc atgaactgcg
    11161 cgaatatgaa aagcgcgatg cctatctgac cgcagtcgag ggcaagtcgg cgggagactc
    11221 caccatgcgt ttgctggcga ccaccaagtt catgctcgat cagcatgatc cgcgtgccgc
    11281 gctgaatacg ctgcagcagt tgcgcgccag cggcgtgaag agtcatgtcg gcgcgctgtc
    11341 cctcgagctt aaggcgcagc agcagatcgg caactgggat ggggtgctgc agaccctcga
    11401 tcagctggag aagcgcaatg cgatcgatgt gaccgtggcg gcgcagctgc gtcagcaggc
    11461 atggctggaa aagattcggc agcaggagga cttggccgga ttgaccgcat gcctgaaaga
    11521 gcttcccggc gaactgaaac ggcgcagcaa agtggcggcc actgccgcac gcgaattgat
    11581 ccggcatggt gactgcccgc tcgcacagca attgctggct gacagtctga atgcacaatg
    11641 ggacagcgaa ctggttgcgc tgtatggcga ctgtcattcc gatggggcgg cagcggacga
    11701 cgtgattccg aaaatcgagc aagccgagaa gtggctcaat cagcataagc gggatgcggg
    11761 attgctgctg gcgctcggca agctgtgtct gcatcagggc ttatggggca aggcgcggaa
    11821 ctatctggat gccagtgtca gtatcgctcc cacaggggca gcctacaccg cgctcgctca
    11881 gctggcggcg cgattgggag aggcggccga ggcatcaaag tattaccagc aggcggcggg
    11941 actcagaagc gaggattgag gccgaatgtc tttgcggtct ctagcccttg ggtgcaaagg
    12001 tgaaggcgtc cgagaagaag gcatcatccg gtaagccgcg ctggctcgtg aagtcacggt
    12061 gtgccgcttc taccaccacc ggcgcgccgc agacataggt ctcatatccc gacagatcct
    12121 gatagtcgtc cagtaccgcc tgatgcacga agccggtgcg cccctgccag ccgtctgttg
    12181 ctgtcggttc ggaaagcacg ggggtgaact tgatgccgtt gctttcccat agccgggcgg
    12241 tttccagcat gtacagatct gccagtgtgc gagcgcccca gtagaaatgc atcgggcgtt
    12301 tgacgccgat atacagggcg tgctcgatga tggccttgat cggcgcgaag ccggtgccgc
    12361 tggcgacgaa gatgatgggc ttatcggaat cttcgcgcag gaagaagctg cccagcggac
    12421 ccttgaggcg caggatgtcg cgctccttca tgtcgctgaa cacatgctgg gtgaagctgc
    12481 cgcccgcgat attgcggata tgcagttcga gcagatcgtc atggtggggc gcattcgcca
    12541 gcgagaagct gcggggtttc tgatccttct gcagaatgtc gatgtactgt ccggcgagga
    12601 attgcaggcg ctcgttggct gggagcttga gcttcagcac catgacgtcc ggcgcgactt
    12661 tttccatctt gtcgatgcgg cagggcatgg tcttgacctg aatatccttg gtcgcggtta
    12721 tctcgcggct ctcgatgatc aggtcggtca aaggttttgc gcagcagaac aaggccatgc
    12781 cagcctcttt ttccgcgtcg ctcagggcgt ggggctgata gttgccgtaa tcgacttcgc
    12841 cttcaattac cttgcctttg cacacgccgc aagcgccatt ctggcagcta tagggcaggt
    12901 tgcagccgtg cttcaggctg gcttcgagta tcgtttcatc cgcctcgacg gaaaagcggt
    12961 ggttgctggg caggatggtg gtgttgaatg tcatggcagg gagtcaaaat tcttgaagcg
    13021 cggcatttta acgcataatc gcaccatgaa acgcttgctg atcgtaggtt gcggcgatat
    13081 cgcgctgcgc attctcccgc tgttgctccc ccattaccgg gtatatgcgc tgctgcgcaa
    13141 tcaggcgcgg catgccgagt tgcgcgcgag gggggcgatt ccgctggcgg gcgatctgga
    13201 tgaccgtgcc agcctcgcgc gtatcgccgg gctggccgag gcggtattgc atcttgcgcc
    13261 gcctgcgaat accggaacgg tggatgcgcg cacgcgcaac ctgctggccg cgctctcgct
    13321 ggcgaagccg ccaaggcgat tcatatacat cagcaccagc ggcgtgtacg gagactgcgc
    13381 cggggcaggg gtgagtgaga ctcggccggt caatccgcaa aacagcaggg cgcagcggcg
    13441 cgtggatgcc gagcaacgta tccgggaatg ggctgcccga aaccgggtgc gggccagcat
    13501 cctgcgcgtg cccggtatct acgccgcaga tcggctgccg ctggaacgtc tgcgcgcgaa
    13561 taccccggcc atcgtcacgg cggaagacgg ctataccaat catattcatg ccgacgatct
    13621 cgcccgcatc gcgatcgccg cattgcaccg tggcaaagcg aatcgggtct atcacgccag
    13681 tgacgatagc gagttgaaga tgggcgacta cttcgatgca gtggccgatg cgcatggctt
    13741 gccccggcca ccccgcctgt cgcgcgcaga agtcgaacgc agcgtgtccc cgatgctgtg
    13801 gtcgttcatg aacgagtcgc ggcgcttgac caaccagcgg atgaaagttg aattgcaagt
    13861 cgcactgcgc tatccgaccg tggtcgatgc ccttgagggg cgggttaatc gggctggaaa
    13921 agagcgcatg ccgctaatct tcattgattg accgcccatc atttttgccg acccgtttgc
    13981 gacagattgc tcctgcggct cttggcaaat agactcgcgc catctgaaat gcaggagcga
    14041 gcaatgaaaa atgcaaaagg ttttactctg gtcgagctga cgatcgtggt gatcggcatt
    14101 ctggccagca tcggcatacc ggcttacagc gactactcga cgcgcgggaa attgatggag
    14161 gccacctccg gcttcaccgg gaagcgggtg atctggcgtg agttgatacc gtaagcagtg
    14221 gttccaataa cggcggcctt cgggccgctg ttttattccg cccgggat
//
LOCUS       DASUIV010000325         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_737018, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000325 DASUIV010000000
VERSION     DASUIV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737018"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..689
                     /gene="hisG"
                     /locus_tag="VFW59_11580"
     CDS             51..689
                     /gene="hisG"
                     /locus_tag="VFW59_11580"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX5364899.1"
                     /translation="MITIALSKGRIFEETMPLLNAAGIIALEDPESSRKLILPTNRAD
                     VRLIIVRASDVPTYVQYGAADMGVAGKDVLNEHGGTGLYQPLDLNIAKCRMMVAVRND
                     YDYESAVRQGARLRVATKYVQTAREHFAAKGVHVDLIKLYGSMELAPLVGLSDVIVDL
                     VSSGGTLKANDLKAVEHIADISSRLVVNQAALKLKHAVIQPMLDAFAGAVKK"
     gene            700..1995
                     /gene="hisD"
                     /locus_tag="VFW59_11585"
     CDS             700..1995
                     /gene="hisD"
                     /locus_tag="VFW59_11585"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5364900.1"
                     /translation="MNIRRLSSRTADFGAALDRLLAFEETADEKLEATVAAILADVRQ
                     RGDEAVLECTRKFDRLPLANAAAMELPRDELRAAFDGLPARQRIALEQAAQRVTDYHQ
                     KQVQPSWSYTDADGTLLGQQVTPLDRVGLYVPGGKAAYPSSVLMNALPAKVAGVGELI
                     MVVPTPDGVKNQLVLAAAYLSGVDRVFTIGGAQAVAALAYGTATIPKVDKIVGPGNAY
                     VASAKRRVFGVCGIDMIAGPSEILVICDGKTDPDWIAMDLFSQAEHDELAQAILLSPD
                     AGFIEAVAQSADRLLEQMPRKDIIRTALENRGALIHVADMEEACAISNRIAPEHLELS
                     VEEPQVWLPRLRHAGAIFMGRYTSESLGDYCAGPNHVLPTSGTARFSSPLGVYDFQKR
                     SSLIQVSREGAQHLGAIATELAMGEGLHAHAQSARYRLG"
     gene            2076..2160
                     /locus_tag="VFW59_11590"
     tRNA            2076..2160
                     /locus_tag="VFW59_11590"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2110..2112,aa:Tyr,seq:gta)
     gene            2226..2299
                     /locus_tag="VFW59_11595"
     tRNA            2226..2299
                     /locus_tag="VFW59_11595"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2258..2260,aa:Gly,seq:tcc)
     gene            2312..2386
                     /locus_tag="VFW59_11600"
     tRNA            2312..2386
                     /locus_tag="VFW59_11600"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2344..2346,aa:Thr,seq:ggt)
     gene            2432..>2569
                     /locus_tag="VFW59_11605"
     CDS             2432..>2569
                     /locus_tag="VFW59_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5364901.1"
                     /translation="MAKSKFERTKPHVNVGTIGHVDHGKTTLTAAITMVLSKKFGGEA
                     KA"
BASE COUNT          516 a          626 c          846 g          581 t
ORIGIN      
        1 cgcaggttga aataacagat tcgtcgcatt aaataaatag gaattttcaa gtgataacca
       61 tcgcactgtc caagggacgc atcttcgaag aaaccatgcc gttgctgaat gcggcgggca
      121 tcattgcgct ggaagatccg gaatcgtcgc gcaagctgat actgccgacc aatcgcgccg
      181 acgtgcgttt gattattgtg cgtgccagcg atgtgccgac ctatgtgcaa tatggcgcgg
      241 ccgacatggg ggtggcgggc aaggatgtgt tgaacgagca tggcggcacg ggcttgtacc
      301 agccgctgga tctgaatatt gccaagtgcc gcatgatggt ggcggtacgc aatgattatg
      361 attacgagtc ggcggtgcgt cagggcgcgc gtctgcgcgt ggcgaccaag tatgtgcaga
      421 ccgcgcgcga acactttgcg gcgaaggggg tgcacgtcga tctgatcaag ctgtacggtt
      481 cgatggagtt ggcgccgctg gtcggtctgt ccgatgttat cgtcgatctg gtgagcagcg
      541 gcggaacgct caaggccaat gatctgaagg cggtcgagca tatcgccgac atctcgtcgc
      601 gactggtggt gaatcaggcg gcgctcaaat taaagcatgc cgtgatccag ccgatgctgg
      661 atgcttttgc tggcgcagtg aagaagtagg aggggcagaa tgaacatcag acgactttct
      721 tcgcgtacgg cggatttcgg tgcggcactg gacaggttgc tggccttcga ggaaacggca
      781 gacgagaagc ttgaggctac cgtggctgcc atccttgcgg atgtgcgtca gcgcggagac
      841 gaggccgtgc tggagtgcac gcgcaagttc gaccgtttgc cgctggcgaa tgccgccgcg
      901 atggagttgc ctcgcgatga attgcgcgcg gcgttcgacg ggctgcctgc caggcagcgc
      961 atcgcgcttg agcaggcggc gcagcgagtc accgattacc atcagaagca ggtgcagccc
     1021 tcctggagct atacggatgc cgatggcacc ttgctcggtc agcaggtgac gccgctggat
     1081 cgggttggct tgtatgtgcc gggcggcaag gcggcttacc cttcatctgt gctgatgaat
     1141 gctttgccgg ccaaggtggc gggcgtcggc gagctcatca tggtggtgcc gacgccggat
     1201 ggtgtgaaga atcagttggt gctggcggcc gcttatctgt cgggcgtgga tcgcgtgttt
     1261 accatcggcg gtgcgcaggc tgtggcggcg ctggcctatg gcacggcaac catccccaag
     1321 gtcgacaaga tcgtcggtcc tggcaacgcc tatgtggctt ccgccaagcg ccgtgtcttc
     1381 ggcgtgtgcg gtatcgatat gatcgcgggg ccgtccgaga tactggtgat ctgcgatggc
     1441 aagaccgatc cggactggat cgcgatggat ctgttctcgc aggccgaaca tgacgaatta
     1501 gcgcaggcta ttttgttgtc gcctgatgca gggtttattg aggctgtggc acaaagtgcg
     1561 gatcggttgc tggagcagat gccgcgcaaa gatatcatca ggaccgcgct ggagaatcgt
     1621 ggcgcactga ttcatgtggc ggatatggaa gaggcctgcg ctatcagcaa ccgaattgcc
     1681 ccagagcatc tggagttgtc ggtcgaagag cctcaggtat ggttgcccag gttgaggcat
     1741 gctggtgcca ttttcatggg gcgctacacc tccgaatcgc tgggtgatta ttgcgcaggc
     1801 ccgaaccatg tattgccgac ttccggtacc gctcgattct cttccccgtt gggggtgtat
     1861 gacttccaga agcgtagcag cctgattcag gtgtcgcgtg agggtgcgca gcatctgggc
     1921 gcgatcgcca cggagcttgc gatgggagaa ggattgcacg cgcacgcgca gtctgctcga
     1981 tatcgtctgg ggtgattggg gcgtgttcgt gatgtaatga tgtcaaattg atttgacaag
     2041 gttattgagc atcggcataa tgccgggctc agtttggagg ggttcccgag cggtcaaagg
     2101 gatcagactg taaatctgac ggctcagcct tcgaaggttc gaatccttcc ccctccacca
     2161 agttttgtgt gtatttgagt tgggatgtaa tgacggcttg tgtgttattg cattgagggg
     2221 tcgtcgcggg tgtagctcaa tggtagagca gaagttttcc aaacttacga cgagggttcg
     2281 attcccttca cccgctccag tataaatgtc tgcccatgtg gctcagtggt agagcactcc
     2341 cttggtaagg gagaggtcgc gagtccgatt ctcgccatgg gcaccagatt ttgtatttat
     2401 tattaacttt tgacattgat aaggaaccat catggcaaag agcaaattcg aacgtacgaa
     2461 gccgcacgta aacgtaggca cgatcggtca cgttgaccac ggtaaaacga cgctgacagc
     2521 ggcgatcacg atggtgttgt cgaagaagtt tggtggcgaa gcgaaggct
//
LOCUS       DASUIV010000326         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_552400, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000326 DASUIV010000000
VERSION     DASUIV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552400"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..578)
                     /locus_tag="VFW59_11610"
     CDS             complement(84..578)
                     /locus_tag="VFW59_11610"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HEX5364902.1"
                     /translation="MRNHSASVVVRLLAGIVLMTAGTAFAAQPVKADDMGLSKGSVFD
                     TPTPRVYHYGTAQPGQSKLLPRAYSGAPPQIPHDISDFLPITAQSNMCIACHNQPAQW
                     GKAPEPGVATAIPASHYTDLRRAPGKVTENLINARYNCNQCHVPQTDAPALVKNTFST
                     KRGR"
     gene            complement(608..>3327)
                     /gene="napA"
                     /locus_tag="VFW59_11615"
     CDS             complement(608..>3327)
                     /gene="napA"
                     /locus_tag="VFW59_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HEX5364903.1"
                     /translation="DFLKQSVIVSTAAMTVGLPVAEAAQAETQNAEAGWRWDRGVCRF
                     CGVGCGIQIATEKGRIVATKADVDAPVNRGLNCIKGYFNGKIPYGQDRLTQPLLRMKN
                     GHFDKQGDFVAVSWERAFDEMEKQFKRHYRELGPTGVGFFASGQHTVQEGYAAVKLFK
                     AGFRSNNLDPNARHCMASAVAGFMQTFGIDEPSGNYDDIEHTDTMVLWGANMAEMHPV
                     LWTRITDQRMKDPNVRIVNLTTISNRSSDIADLEIIFKPSTDLAIMNYLAREIVTRDA
                     VNWEFVNKHCAFAAGPTDIGYGMRGSDKFDYAAEKDTNGKEMSMRLDGAEAIAQGKQA
                     GATVAQNNRATPDKHWLITFEDFKKGVEPYTLDFVAELAKGDADEPLGDFKAKLKLLA
                     DLYIEKSRKVVSFWTMGFNQHQRGTWVNEQAYMVHLLLGKQAMPGNGAFSLTGQPSAC
                     GTAREVGTFSHRLPADMVVNNPDHRAASEKIWKLPARTLNPKVGSHFTKLLRDLEDGQ
                     VKWAWVQVCNPFQDTANANHWIKAAREQDNFIVVSDVYPGISTKVADLILPSAIIFEK
                     WGGYGNAERRTQLWREQVPPPGIARSDIWQIMEFSKRFKLKEVWGEQKIPGLKEAGFD
                     DGKLPDVLTGSGHSPDATLYQVLFATPDNRKFKWPDAVAKGHDNHTATLLGDHWFPEK
                     ALFEEYAQFGRGHAHDLADFDVYFRDDVRGLKWPVVNGKETHWRFNEAYDPYVKKGSG
                     FDFYGSAFKSLPSGNLDGVTNNEKTSIAGRAKIFFRPYAAPVEQPDANYDMWLSTGRV
                     LEHWHSGTMTRRVRELHWAVPAALIYMHPADAKARGLKQDDIAWIESRRGKVKAAVDT
                     KGRNRPPKGLVFVPWFDEGVFINKVTLDSTCPISKQTDFKKCAVKIYKA"
BASE COUNT          622 a         1011 c          966 g          728 t
ORIGIN      
        1 accacgcccg gcagtacgat gcccgcccgg agactaccgc atgaaggaga ccaacatttc
       61 ccaacacgcg gcacgacaag acctcatcgg ccgcgtttgg tggagaaggt atttttcacc
      121 agcgccggtg catctgtctg gggaacatgg cactgattgc agttatagcg cgcgttgatc
      181 agattctcgg ttactttgcc cggtgcccgg cgcagatcgg tgtaatgact ggccgggatg
      241 gccgtggcga cacccggttc aggtgccttg ccccattgcg cgggctggtt atggcaggcg
      301 atacacatgt tgctttgtgc tgtgatcggc agaaaatcgc tgatgtcgtg cgggatttgc
      361 ggcggtgcgc ccgaataggc gcggggcagc agcttgcttt gccccggctg agccgtaccg
      421 tagtgataca ccctgggggt cggggtatcg aacacactcc ccttgctcaa gcccatatcg
      481 tccgccttga cgggctgtgc cgcgaaagcc gtcccggccg tcatcagaac gatgccagcc
      541 aatagcctga cgacgacgga tgcagagtgg ttgcgcattt tgtttctcct tggcgagttc
      601 attcaggtca ggccttgtat atcttcacgg cacatttctt gaagtcggtt tgcttggaaa
      661 tcggacaggt ggaatccagc gtcaccttgt tgatgaacac gccctcatcg aaccagggca
      721 cgaacaccaa gcccttgggc ggcctgttgc gccctttggt atccaccgca gccttgacct
      781 tgccgcgacg cgactcaatc cacgcgatgt cgtcctgctt gaggccgcgc gccttggcat
      841 ccgccgggtg catatagatc agcgcggcgg gaacggccca gtgcaattcg cgcacacgcc
      901 gcgtcatggt cccggagtgc cagtgctcca gtacacggcc ggtggatagc cacatgtcgt
      961 agttcgcatc cggttgctcc accggtgccg cataggggcg gaagaaaatc ttggcacggc
     1021 ccgcgatgct ggtcttctcg ttattggtca cgccgtccag attgccgctg ggcagcgact
     1081 tgaatgcgga gccgtaaaaa tcgaagccgc tgcctttttt gacgtaggga tcgtaggctt
     1141 cattgaaacg ccagtgcgtt tccttgccgt tgacgaccgg ccatttcagt ccgcgcacat
     1201 cgtcgcggaa gtacacgtcg aaatctgcca gatcgtgggc gtgaccgcgt ccgaactgcg
     1261 catattcctc gaacagggcc ttctcgggga accagtgatc gcccagcaat gtcgcggtgt
     1321 gattgtcgtg ccccttggcg accgcatcgg gccatttgaa tttgcgattg tcgggcgtcg
     1381 cgaacagcac ctgatacaag gtcgcatcgg gcgaatgccc cgatccggtc aacacatcgg
     1441 gcaacttgcc atcgtcgaaa cccgcttcct tcaaccccgg tatcttctgc tcgccccaga
     1501 cctctttcag cttgaagcgc ttggagaatt ccatgatctg ccagatgtcg gaacgcgcga
     1561 tccccggcgg cggcacctgc tcgcgccaca gctgggtgcg ccgttcggca ttgccgtagc
     1621 ccccccattt ctcgaagatg atcgccgagg gcaggatcaa gtccgccacc ttggtagaga
     1681 tgccgggata gacatccgac accacgatga aattgtcctg ttcgcgcgcg gccttgatcc
     1741 agtgattggc gttggcggta tcctgaaacg ggttgcacac ctgcacccag gcccatttga
     1801 cctgaccgtc ttccagatcg cgcaacagct tggtgaaatg gctgccgact ttgggattca
     1861 gcgtcctggc cggcaatttc catatcttct cggaagcagc acgatggtcg ggattgttca
     1921 ccaccatgtc tgccggcagg cggtgggaga aggtgcccac ctcacgcgcg gtaccgcagg
     1981 cggaaggctg tccggtgagg gagaatgcgc cgttaccggg catcgcctgt ttgcccagca
     2041 acagatgcac catataggcc tgctcgttga cccaggtgcc gcgctggtgc tggttgaaac
     2101 ccatggtcca gaagctcacc accttgcggc tcttctcgat atacagatcg gccagcagct
     2161 tgagcttagc cttgaagtcg cccagcggct cgtccgcatc gcccttggcc agctcggcca
     2221 caaaatccaa ggtgtagggc tcgacgccct ttttgaaatc ttcaaaggtg atcagccagt
     2281 gcttgtccgg cgtggcacgg ttattttgcg ccacggtcgc accggcttgc ttgccctggg
     2341 caatggcctc cgcaccatcc agccgcatgg acatttcctt gccgttcgtg tctttctcgg
     2401 cagcgtagtc gaatttgtcc gagccgcgca taccgtaacc gatgtcggtc ggaccggcgg
     2461 cgaaggcaca gtgcttgttg acgaattccc agttcaccgc gtcccgcgtg acgatctcgc
     2521 gcgccagata attcatgatc gccagatcgg tgctcggttt gaagatgatc tccagatcgg
     2581 caatgtcgga agagcggttg ctaatggtgg tcaggttgac gatccgcacg tttggatctt
     2641 tcatgcgctg gtcggtgatg cgagtccaca acaccggatg catctccgcc atgttcgctc
     2701 cccacaacac catggtatcg gtgtgttcga tgtcgtcgta gttgccggag ggttcgtcta
     2761 taccgaaggt ctgcatgaat cccgccacgg cggaagccat gcagtgacgc gcattggggt
     2821 cgaggttatt gctgcggaag cccgccttga acagcttcac cgcagcatag ccctcctgca
     2881 cggtatgctg tcccgatgcg aagaagccga cgccggtggg gcccagctcc ctgtagtggc
     2941 gtttgaactg tttctccatc tcgtcgaacg cgcgttccca ggaaacggca acgaagtctc
     3001 cctgtttgtc gaagtgtccg ttcttcatgc gcagcagcgg ctgcgtcagc ctgtcctgac
     3061 catagggaat cttgccgttg aaataacctt tgatgcaatt tagcccccgg ttcaccgggg
     3121 catccacgtc agctttggtc gcaacgatcc tgcctttttc agtggcgatc tgaatgccac
     3181 aacccacacc acagaaacga cacacacccc gatcccagcg ccaaccggct tcggcatttt
     3241 gagtctccgc ctgagcggcc tcggcaacgg gaaggccaac cgtcatcgcg gcggtggaga
     3301 caatgacgct ttgcttgagg aaatcgc
//
LOCUS       DASUIV010000327         9802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_737602, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000327 DASUIV010000000
VERSION     DASUIV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737602"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1296)
                     /locus_tag="VFW59_11620"
     CDS             complement(28..1296)
                     /locus_tag="VFW59_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEX5364904.1"
                     /translation="MTVWQQLFTRRMLICVFTGFASGLPLYLLFNLVPAWLRSEHVDL
                     KTIGLFALIQFPYTWKFLWSPLLDRYVVPAFGRRRGWMLLAQVGLLLVIALLGGFSPQ
                     TQMSTIVLFCTLLAFLSATQDIVLDAYRRELLSDVELGLGNSVHVNAYRIAGLVPGSL
                     SLILADTLPWDMVFIVTALFMLPGIAMTLSVKEPLNVIPPKSLRDAVVEPFREFISRQ
                     GWNSALLILAFLFFYKLGDSMCTSLATPFYMDMGFTKTQIGLIAKNAGLWPAVIGGLL
                     GGLWMVRIGINRALWLFGVVQVVSIFGFAWLASVGYHSEITGVELAQLALVIGLEALG
                     VGLGTAAFVAFIARSTHPAYTATQFALFTSLAAMPRTFANAATGWLVEILGWMGFFLL
                     CALLAIPGMLLLLKVAPWNERPADAVGRAQ"
     gene            complement(1410..2027)
                     /gene="metW"
                     /locus_tag="VFW59_11625"
     CDS             complement(1410..2027)
                     /gene="metW"
                     /locus_tag="VFW59_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX5364905.1"
                     /translation="MSLRQYLSPTAADRPDFAAIAEWIPPGASVLDLGCGDGSLLRYL
                     KEARGVRGYGVEINDMDIVMCIANGVNVIQNDLESGLSDFEDNAFDFVILSQTLQATR
                     HTEALIREMLRVGREGIVSFPNFGYWKNRLNVLTGHMPVSRDLPYQWYDTPNVHLCTL
                     HDFEAFSREHRIAILDRSVMTGEKEVGLLPNLLGSTAVYRFQRGI"
     gene            complement(2024..3163)
                     /locus_tag="VFW59_11630"
     CDS             complement(2024..3163)
                     /locus_tag="VFW59_11630"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX5364906.1"
                     /translation="MSTTQSIGVVRARRAQFDTPLTFRSGAVLPRYELVYETYGKLNA
                     DKSNAILICHALSGHHHVAGYYADEPKNVGWWDNMVGPGKPINTDKFFVIGLNNLGGC
                     HGSTGPSSVDPETGQPYGASFPVITVEDWVESQARLADLLGIKQFAAVVGGSLGGMQA
                     LQWSLAYPERVRHVLAIASAPRLTAQNIAFNDVARNAILTDPDFHGGNFYQHGVVPTR
                     GLRLARMLGHITYLSDDAMADKFGRELRTGKLNYGYDVEFQIESYLRYQGDKFAGFFD
                     ANTYLLMTKALDYFDPSQDLGGDMNQAFARARADFLVISFTTDWRFSPQRSREIVQPL
                     LHNRRNVSYAEITSTHGHDSFLMEHPHYFDVMRAYLDNVASEFSA"
     regulatory      complement(3204..3278)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(3518..5242)
                     /gene="ptsP"
                     /locus_tag="VFW59_11635"
     CDS             complement(3518..5242)
                     /gene="ptsP"
                     /locus_tag="VFW59_11635"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX5364907.1"
                     /translation="MSFTLHGIAVSSGIAIGHAHLISHTSLEVAHYALPPHLVADEVV
                     RFDAALQATRDEFTSLRSNRPAHAAAEFDAFLELHQMILDDSLISSAPREMIEREHCN
                     AEWALKVQVETLVAQFDEFEDAYLRERQTDVRQVAERLLKQLIGQPSHQPPSTRHDVD
                     TILVAHDLSPADLIQFKPQQYAAFVTDVGGATSHTAIVARGLSTPCVVGLHHARQLIR
                     EDDLLIVDGEQGVLIVNPGKSVLAEYKLRQSEWELDRKKLKRLRTARANTLDGTVIEL
                     HSNIEKPEDITEVKENGATGVGLFRSEFLFLNRDNLPDEEEQFQAYRAAAEGMDGQPV
                     TIRTFDLGADKQIKGAERVANNPALGLRAIRLCLAEPMLFRTQLRALLRATQYGNIKI
                     LIPMLSSVSEINQTLLFIEATKYSLEIDGIPFDREVKIGGMIEIPAAALSVAAFAKKL
                     DFLSIGTNDLIQYTLAIDRTDEEVAHLYDPLHPGVLQLLSSVIATCNKLDVPISVCGE
                     MAGELPYTRLLLGMGLRQFSMFSAQVPNIKQRVLTTSLPEIAALTQKILRADDPLKIR
                     ELLDRLNA"
     gene            complement(5239..5508)
                     /locus_tag="VFW59_11640"
     CDS             complement(5239..5508)
                     /locus_tag="VFW59_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX5364908.1"
                     /translation="MQQQDVLIINKLGLHARASAKLTQMASGFKCEVMLSRNNRRVNA
                     KSIMGVMMLAASKGTTIVIETDGPDEAEAMQALQALINDYFGEGE"
     gene            complement(5514..5924)
                     /locus_tag="VFW59_11645"
     CDS             complement(5514..5924)
                     /locus_tag="VFW59_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEX5364909.1"
                     /translation="MGVIQVVGILLVTHNGLGGSLLNCVEHVLGEVPHNLKALSVEAS
                     DDPQQKLVEGQALIKELDTGAGVLILADVYGATPSNIGRQLCHAERVMGVAGVNLPML
                     LRSVCCSNVNLPELAEAAIKGGRDCIVHMNDELG"
     gene            complement(5941..7008)
                     /locus_tag="VFW59_11650"
     CDS             complement(5941..7008)
                     /locus_tag="VFW59_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5364910.1"
                     /translation="MSYVCIVMFALLLAACGKEPLYQEQGYVFGTLVDVSIYGEDDAT
                     ARQAVSEVLHEFQRLHDELHAWQPSELSDLNAAFAKGESKAVSPELSTMLQDAAQLSA
                     QSQGLFNPTIGGLIQLWGFQSDEFKPQLPDEKRVAALVAANPQMSDLQFLPSTLSPDP
                     SPASGRGEFVRSKNKAVKLDLGGYAKGYALDRAAQLLRKRGIHHALINIGGNIMALGQ
                     HGKRPWRVGIQHPRQSGALASLELHDGEAIGTSGDYQRYFELNGKRYCHLIDPRSGHP
                     VQGVRAVTVLTRGARAGVLSDVVSKPLYIAGLAGWRAAAQRMALTEAMLVDAQGNIHL
                     TAALQKRLEFADKNIQVEVSP"
     gene            complement(7110..8381)
                     /gene="gshA"
                     /locus_tag="VFW59_11655"
     CDS             complement(7110..8381)
                     /gene="gshA"
                     /locus_tag="VFW59_11655"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX5364911.1"
                     /translation="MVPHLTTALTGPLLDLERKFLAAQPTIEHWLRAQWQEHAVPFYA
                     SVDLRNSGFKLAPVDTNLFPGGFNNLNQDFLPLCVQAMQSAVEKICPEARGVLLIPEN
                     HTRNQFYLQNVKHLVTILERAGMRVRVGSLLPEITEPTAISLPDGRTLMLEPLQRRGN
                     RIGLADFDPCVVLLNNDLSGGVPDILKNLEQSVFPPLSAGWYVRRKSQHFAAYDRVAN
                     EFAALLGIDPWLINPYFAVCNQINFQERIGEACLAAQVDDVLGKMRAKYAEYGVKHDP
                     FVIVKADAGTYGMGIMTVKDASEITGLNRKQRNKMAVIKEGQEVHDVLIQEGVYTFEN
                     INDAVAEPVIYMIDRYVVGGFYRVHTDRGMDENLNAPGMRFEPLAFESCCSLPNPDCA
                     PDDTPNRFYAYGVVARLALLAASLELEGSAE"
     gene            8555..>9802
                     /locus_tag="VFW59_11660"
     CDS             8555..>9802
                     /locus_tag="VFW59_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEX5364912.1"
                     /translation="MLSTANITMQFGAKPLFENVSVKFGDGNRYGLIGANGCGKSTFM
                     KILGRDLVQTSGEVMLDKTDRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMSERDAI
                     YANPDASEEDYMRAADLEATFAEYDGYTAEARAGELLLGLGIDIKLHNGPMREVAPGF
                     KLRVLLAQALFSNPDILLLDEPTNNLDINTIRWLEDILNARTCTMVIISHDRHFLNSV
                     CTHMSDLDYGKITTYPGTYDDYMMASAQAREQQSADNAKAKEKVAELKAFVARFSANA
                     SKAKQATSRARQIDKIKIEDIKPSSRQYPFIRFEYDEREKLHRTAVEVEALSKGFDRV
                     LFSNVNFRIDAGEKVAIIGPNGIGKTTLLRCLAGDLAPDYGTIKWTDKAKIGYYAQDH
                     TADFAEDKSMTEWMTYWRGPHDDD"
BASE COUNT         1941 a         3074 c         2985 g         1802 t
ORIGIN      
        1 atgattggcg cgccgcatcg ggcgcattca ttgcgccctg ccgaccgcgt ccgctggccg
       61 ctcgttccag ggcgcgacct tgagcagcag cagcatgccg gggatggcga gcagcgcgca
      121 cagcaggaag aaccccatcc accccagtat ctccaccagc cagccggtcg ccgcgttggc
      181 gaaagtgcgc ggcatcgcgg cgaggctggt gaacagcgcg aactgggtgg cggtgtaggc
      241 agggtgggtg ctgcgggcga tgaaggcgac gaaggcggcg gtgcccagcc caacgcccaa
      301 ggcttcgagg ccgatcacga gcgcgagctg ggcgagttcg acgccggtga tttcactgtg
      361 ataccccacc gaggccagcc aggcgaaacc gaagatggac accacctgca ccacgccgaa
      421 cagccacagc gcgcggttga tgcctatcct caccatccac aggccgccga gcaagccgcc
      481 gatcaccgca ggccacagcc cggcattctt ggcgatcagg ccgatctggg tcttagtgaa
      541 acccatgtcc atgtaaaacg gcgtggccag cgaggtacac atgctgtcgc cgagtttgta
      601 gaaaaacagg aaggctaaga tcagcagcgc gctgttccag ccctgccgcg agatgaattc
      661 gcggaacggc tcgaccaccg catcgcgcag actcttcggc ggaatgacgt tgagcggctc
      721 cttgaccgac agggtcatcg ctattcccgg cagcatgaac agcgcggtga cgatgaacac
      781 catatcccag ggcagggtat ccgccaggat cagcgacagc gaacccggca ccagccccgc
      841 gatgcgatag gcgttcacat gcaccgagtt gcccagcccc agctcgacat cggagagcag
      901 ttcgcggcga taggcatcca gcacgatgtc ctgcgtggcg ctgaggaagg cgagcagcgt
      961 gcagaacagc acgatggtag acatctgggt ctgcggcgag aatcccccga gcaacgcgat
     1021 aaccagcaac agtccgacct gcgcgagcag catccagccg cgccgtctgc cgaaggcggg
     1081 cacgacgtag cggtcgagca gcggcgacca caggaacttc caggtgtagg gaaactggat
     1141 caacgcgaac aggccgatgg tcttgagatc gacatgctcg ctgcgcagcc aggcggggac
     1201 gagattgaac agcagataca gcggcagccc ggaggcgaaa ccggtgaata cgcagatcag
     1261 catgcgacgg gtgaagagtt gctgccagac ggtcatggaa tcattttcga agccaccctc
     1321 tccctaaccc tcccccgcct gcgggggagg gaatgatctc tcccctcgcc cacaagtggg
     1381 agaggggttg ggggagaggg cgcacagaat taaatcccac gctggaagcg atacaccgct
     1441 gtgctgccca gcaggttggg caacaggccg acttcctttt cgccggtcat cacgctgcga
     1501 tccaggatcg cgatgcgatg ctcgcggctg aaagcctcga agtcatgcag cgtgcacagg
     1561 tggacgttgg gcgtgtcgta ccactggtag ggcaggtcgc gcgacaccgg catgtggcct
     1621 gtcagcacgt tcaggcggtt cttccagtag ccgaagttgg ggaagctgac gatgccctcg
     1681 cgcccgacgc gcagcatctc gcggatcagc gcctcggtgt ggcgcgtggc ctgcagcgtc
     1741 tgcgacagga tcacgaagtc gaacgcgttg tcctcgaaat cggacaggcc ggattccaga
     1801 tcgttctgga tcacgttcac gccgttggcg atgcacatca cgatgtccat atcgttgatc
     1861 tccacgccgt agccgcgcac gccgcgcgcc tccttcaggt aacgcagcag gctgccgtcg
     1921 ccgcaaccga ggtcgagcac cgaggcgccc ggcggtatcc actcggcgat ggcggcgaag
     1981 tcgggacggt cagcggcggt gggggagaga tactggcgca ggctcatgct gaaaactcgc
     2041 tggcaacgtt atcgaggtag gcgcgcatca cgtcgaaata gtgcggatgc tccatcagga
     2101 aggaatcgtg tccatgcgtc gaggtgatct cggcatagct gacgttgcgc cggttgtgca
     2161 gcaagggctg gacgatctcg cgcgaacgct gcggcgagaa gcgccagtcg gtggtgaacg
     2221 agatcaccag aaaatccgcc ctggcccgcg cgaacgcctg gttcatgtcg ccgcccagat
     2281 cctgcgaggg gtcgaaataa tccagcgcct tggtcatcag cagataggtg ttggcgtcga
     2341 agaatccggc gaacttgtcg ccctgatagc gcagatagga ctcgatctgg aattccacgt
     2401 cgtagccgta gttgagcttg ccggtgcgca gttcgcgccc gaatttgtcc gccatcgcat
     2461 cgtcggacag gtaggtgata tgcccgagca tgcgcgcaag gcgaaggccg cgcgtcggca
     2521 ccacgccgtg ctggtagaag ttgccgccgt ggaaatccgg atcggtcagg atggcgttgc
     2581 gcgccacgtc gttgaaggcg atgttctgcg cggtcagacg cggtgcgctg gcgatcgcca
     2641 gcacatggcg cacgcgctcg gggtaggcca gcgaccattg cagcgcctgc atgccgccca
     2701 gactgccgcc gacgacggcg gcgaattgtt tgatgccgag cagatcggcc agacgcgcct
     2761 gcgactccac ccagtcttcc acggtgatca ccggaaagct cgcgccgtaa ggctggccgg
     2821 tctcgggatc gacggaggaa ggtccggtcg aaccgtggca gccgcccagg ttgttcagcc
     2881 cgatcacgaa gaacttgtcg gtgttgatcg gcttgcccgg ccccaccatg ttgtcccacc
     2941 agcccacgtt cttgggctcg tcggcatagt aacccgcgac gtggtggtga ccggacagcg
     3001 cgtggcagat caggatggcg ttggacttgt cggcgttcag tttgccgtag gtctcataca
     3061 ccagctcgta gcgcggcagc accgcgccgc tgcgaaacgt cagcggtgta tcgaattgcg
     3121 cgcgccgtgc acggacgacg cctatgctct gtgttgtact caagctcgta gccccaaaaa
     3181 caaaacccgt tcagcttgcg ccaaaacggg ttgcctcgct ttagctggaa tgtttaacgt
     3241 gccccgcaag ctgatgtcaa atcggcactg ataaaactac tggccattcg actaaacgag
     3301 taaacaacac tcattaagtc gctggtcata agttgcagtt tccagttttt gcggaacgtt
     3361 gtcaatcgac agcattgaga tggcaggggc acggcggtgt caactccaca tccagcttgc
     3421 tggatataac cccccacttg gcaaccgttt cctgattgct tagtgggatg gctagttgtt
     3481 ctatcagtgg attgcctaac tttgttgcgc cgtgccccta cgcattcagc ctgtccagca
     3541 gctcgcgtat cttcagcggg tcgtcggcac gcaatatctt ctgcgtcagc gccgcgatct
     3601 ccggcaggct ggtggtcagc acgcgctgct tgatgttcgg cacctgcgcc gagaacatcg
     3661 aaaactggcg caaccccata cccagcagca ggcgcgtgta aggcagctcg cccgccattt
     3721 cgccgcacac cgaaataggc acgtcgagtt tattgcaggt cgcaatgacg ctcgacagca
     3781 attgcagcac ccccggatgc agggggtcgt acagatgcgc gacctcctcg tcggtacggt
     3841 cgatcgccag cgtgtactgg atcaggtcgt tggtgccgat agacaggaaa tcgagcttct
     3901 tggcaaaggc cgcaaccgac agcgcggcgg cgggtatctc gatcatgccg ccgatcttga
     3961 cctcgcggtc aaaggggatg ccgtcgatct ccagactgta tttggtcgcc tcgatgaaca
     4021 gcagcgtctg gttgatctcg gacacgctgg acagcatcgg aatcaggatc ttgatgttgc
     4081 cgtactgcgt ggcacgcagc agcgcgcgca actgggtgcg gaacagcatc ggttcggcca
     4141 ggcacaggcg gatcgcccgc agccccagcg ccgggttgtt ggcgacgcgc tcggccccct
     4201 tgatctgctt gtcggctccg aggtcgaagg tgcggatggt gaccggctgt ccgtccatgc
     4261 cctctgcggc ggcgcgatag gcctggaact gctcttcctc gtcgggcaga ttgtcgcgat
     4321 tcaggaacag aaactcgctg cggaacaggc ctacgccggt cgcaccgttc tcttttactt
     4381 cggtgatgtc ttcgggcttc tcgatattgg aatgcaattc gatcaccgtg ccgtccagcg
     4441 tgttggcgcg cgcggtgcgc agccgcttga gcttcttgcg atccagttcc cactcgctct
     4501 ggcgcagctt gtactcggcc aggacagact tgcccggatt gacgatcagc acgccctgtt
     4561 cgccgtctac gatcaacaga tcgtcttcgc ggatcagctg gcgcgcatga tgcaggccaa
     4621 ccacacaggg cgtgctcagg ccgcgcgcaa cgatcgcggt atgcgaagtc gcgccaccca
     4681 catcggtgac gaaggcggca tattgttgcg gcttgaactg gatcaggtcg gcggggctca
     4741 ggtcgtgagc gaccagtatc gtatcgacat cgtggcgcgt cgacggcggc tgatggctgg
     4801 gctggccgat gagttgcttg agcaggcgct ccgcgacctg cctcacatcg gtctggcgct
     4861 cgcgcaggta ggcgtcctca aactcgtcga actgcgcgac cagtgtctcg acctgcacct
     4921 tgagcgccca ctccgcgttg caatgctcgc gctcgatcat ctcgcgcggc gcgctgctga
     4981 tcagcgaatc gtccagaatc atctggtgca gttcgaggaa ggcgtcgaat tcggcggcgg
     5041 cgtgtgcagg acggttgctg cgcagactgg tgaattcgtc gcgcgtggcc tgcagcgcgg
     5101 catcgaaacg caccacctcg tccgcgacga gatgcggcgg cagcgcgtag tgcgccactt
     5161 ccagcgaagt atgcgatatc agatgcgcat ggccgatggc gatgccgctg gagacggcga
     5221 tgccgtgtag cgtaaagctc actcaccttc tccgaaataa tcgttgatca atgcctgcag
     5281 tgcctgcatc gcttcggcct catccggccc gtcggtctcg atgactatgg tcgtgccctt
     5341 ggaagcggcc agcatcatca cgcccatgat gctcttggcg ttcacgcgac gattgttgcg
     5401 cgacagcatc acttcgcatt tgaagccgga tgccatctgg gtgagtttgg ccgaggcgcg
     5461 cgcatgcagg ccgagcttgt taataatcaa tacgtcttgt tgttgcattc cgcttatccg
     5521 agttcatcat tcatgtgcac gatacagtcg cgccccccct tgatggcggc ctccgccagt
     5581 tccggcaagt tgacgttgct gcaacataca ctgcgcagca gcatcggcag gttcaccccg
     5641 gccactccca tcacccgctc ggcatgacac agttgccgac cgatgttgct gggtgtggcg
     5701 ccatatacgt ccgccagaat cagcaccccg gcccccgtat ccagctcctt gatcaaggcc
     5761 tgaccctcaa ccagtttctg ctgcggatca tccgaggcct ctaccgacag cgccttaaga
     5821 ttgtgcggca cctcgcccag cacatgctcg acgcaattga gcaaactgcc gcccagaccg
     5881 ttatgcgtga ccaacaatat tccgaccacc tggatcaccc ccatcaaaaa gccgtcccat
     5941 tcacggcgat acttccacct gtatgttctt gtcggcgaat tctagccgtt tctgcagcgc
     6001 ggctgtgaga tggatgttgc cctgcgcatc caccagcatc gcctccgtca gcgccatgcg
     6061 ctgcgcggcc gcccgccagc cagccagccc ggcgatatac agcggcttgg aaaccacgtc
     6121 ggacagcacg cctgctcgtg cgccccgcgt cagcactgtc accgcgcgca cgccctgcac
     6181 cggatggccg ctgcgcggat cgatcaggtg gcagtaacgt ttgccgttca actcgaaata
     6241 gcgctgatag tcgccggaag tgccgatcgc ctcgccgtca tgcaactcca gcgaggccag
     6301 cgcaccggac tggcgcggat gctggatgcc gacccgccaa ggccgcttgc catgctggcc
     6361 cagcgccatg atgttgccgc cgatgttgat cagcgcatga tggatgcccc gcttgcgcag
     6421 cagctgcgcc gcgcgatcca gcgcatagcc cttggcataa ccgccgagat cgagcttgac
     6481 cgccttattt ttactccgca cgaactcccc tctcccgctt gcgggagagg ggtcagggga
     6541 gagggtcgag gggagaaatt gcaggtcgct catctgcgga ttggccgcaa ccagtgcggc
     6601 cacccgcttc tcatcgggca actgcggttt gaattcgtcg gactggaaac cccatagctg
     6661 gatcagcccg cctatggtcg gattgaacaa cccctgcgac tgcgcggaga gttgcgccgc
     6721 atcctgcaac atcgtcgaaa gctccggcga caccgccttg ctctcgccct tggcgaaggc
     6781 cgcgttcaga tcgctcagct cactcggctg ccaggcatgc agctcgtcat gcagccgctg
     6841 gaactcatgc aacacctcgc tcaccgcctg ccttgccgtt gcgtcatcct cgccgtagat
     6901 gctgacatcc accagcgtgc cgaacacata gccctgctcc tgatacagcg gctccttgcc
     6961 gcaggcggcg agcagcagcg caaacatgac gatgcaaaca tacgacacac ccctttgcag
     7021 aagaggggat tgaagaccgg cgccattacg ggaaaatccc ccctgccccc cttttgccaa
     7081 gaggggatgg tgcagagcaa ataccgacgt cactcggccg acccctccag ctccagcgag
     7141 gccgccagca gcgccagtct ggccaccacg ccgtaggcgt agaaacggtt gggggtgtcg
     7201 tccggcgcgc agtccggatt gggcagggag cagcaggatt cgaaggccaa gggctcgaag
     7261 cgcatgccgg gggcgttgag gttctcgtcc atgccccggt cggtgtgtac gcggtaaaaa
     7321 ccgccgacga cgtagcggtc gatcatgtag atcaccggtt cggccaccgc atcgttgatg
     7381 ttctcgaagg tgtacacgcc ctcctgtatc agcacatcgt gcacttcctg accttccttg
     7441 atcaccgcca tcttgttgcg ctgtttgcgg ttcagcccgg tgatctcgct ggcgtccttg
     7501 acggtcatga tgcccatgcc gtaggtgccc gcatcggctt tgacgatgac gaagggatcg
     7561 tgcttcacgc cgtattcggc atacttggcg cgcatcttgc ccagcacatc gtccacctgc
     7621 gcggcgagac aggcctcgcc gatgcgctcc tggaaattga tctgattgca caccgcaaaa
     7681 tagggattga tcagccaggg gtcaatgccg agcaaggcgg caaattcatt cgcgactcgg
     7741 tcgtaagcgg cgaagtgctg cgatttgcgc cgcacatacc agccggccga cagtggcggg
     7801 aataccgact gctcgagatt cttcaggatg tcgggcacgc cgccggacag gtcgttgttg
     7861 agcagcacca cgcagggatc gaagtccgcc aggccgatgc ggttgccacg gcgctgcaac
     7921 ggctccagca tcagggtacg gccatccggc aaggagatcg ccgtcggctc ggtgatctcg
     7981 ggcaacaggc tgccgacacg cacccgcatc ccggctcgct cgagaatggt gaccagatgc
     8041 ttcacgttct gcagatagaa ctggttgcgc gtgtggttct ccgggatcag caacacgccg
     8101 cgcgcctccg ggcagatctt ctccactgcg gactgcatcg cctgcacgca cagcggcaga
     8161 aagtcctgat tgagattgtt gaagccgccg ggaaacaggt tggtgtctac cggagcgagc
     8221 ttgaagccgc tgttgcgcag atccaccgag gcatagaacg gcacggcatg ctcctgccac
     8281 tgagcacgca gccagtgttc gatggtcggc tgcgcagcca gaaattttcg ctccagatcg
     8341 agcagcgggc cggtcagtgc ggtagtgaga tgtggaacca tgacgttcgc caaataatta
     8401 actcggtgca ttctagccga tagcccaaat tcagggatga atgcgtcagg ctacagattc
     8461 ggtaaatagg gtctgcagtg gtagaatgcg cccctttaat ttttccggct ccccaattcg
     8521 tctcgcgcct tgagggggcc cgcaaggcaa tattatgtta tctaccgcaa atatcaccat
     8581 gcagttcggc gccaagccgc tgttcgagaa tgtctccgtc aagttcggcg acggcaaccg
     8641 ctacggcctg atcggcgcga acggctgcgg caaatccact ttcatgaaga tcctcggccg
     8701 cgatctggtc cagacctcgg gcgaggtgat gctggacaag accgaccgcc tcggcaagct
     8761 gcgtcaggac cagttcgcct acgaagacat gcgcgtgctg gacgtagtga tgatgggcca
     8821 caccgagatg tgggcggcga tgtcggaacg cgacgcgatc tacgcgaacc cggacgcctc
     8881 cgaggaagac tacatgcgcg ccgccgacct ggaggccacc ttcgccgaat acgacggcta
     8941 caccgccgaa gcgcgggcgg gcgagttgct gctgggtctg ggcatcgaca tcaaactgca
     9001 taacggcccg atgcgcgaag tcgcacccgg cttcaagctg cgcgtgctgc tggcgcaggc
     9061 gctgttctcc aatccggaca tcctgctgct ggacgagccg accaacaacc tggacatcaa
     9121 caccatccgc tggctggaag acatcctgaa tgcgcgcacc tgcacgatgg tgatcatctc
     9181 gcacgatcgc cacttcctga acagcgtatg cacccatatg tccgacctgg actacggcaa
     9241 gatcaccacc tatcctggca cctacgacga ttacatgatg gcgtcggctc aggcgcgcga
     9301 gcagcaatcg gcagacaacg ccaaggccaa ggaaaaggtc gcagagctga aagccttcgt
     9361 ggcacgtttc tcggcgaacg cctccaaggc caagcaggcc acttcgcgtg cgcgccagat
     9421 cgacaagatc aagatcgagg acatcaagcc ttccagccgc cagtatccct tcatccgctt
     9481 cgaatacgac gaacgcgaga agctgcaccg taccgccgtg gaagtcgagg cgctcagcaa
     9541 aggcttcgac cgcgtgctgt tctctaacgt gaacttccgc atcgacgcgg gcgagaaggt
     9601 cgcgatcatc ggccccaacg gcatcggcaa gaccacgctg ctgcgctgtc tggcgggcga
     9661 tctcgcgccg gactacggca ccatcaaatg gaccgacaag gccaagatcg gctactacgc
     9721 tcaggaccat accgcagact tcgccgaaga caagagcatg accgagtgga tgacctactg
     9781 gcgcggcccg cacgacgacg at
//
LOCUS       DASUIV010000328         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_643542, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000328 DASUIV010000000
VERSION     DASUIV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643542"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1459
                     /locus_tag="VFW59_11665"
     CDS             116..1459
                     /locus_tag="VFW59_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX5364913.1"
                     /translation="MHEETQAIHAIFGLNPLWLATFIFVATYAVIISERINRAIVALL
                     GGGLMILCGILTQEAAIRGVDFNTLGLLIGMMVIVSITKKSGVFQFVAIWIAKQVKAH
                     PVGIMLMMSLVTALFSALLDNVTTVLLVTPVILLITEELKIKPYPYLFAIIFMSNIGG
                     TATLIGDPPNIMIGSAANLSFNDFLLNLTPIIPLIAIATLLPIWWWWRKDMIAAPEDR
                     ARVMRFNEIEAITDWKLLKQALLVFSLVIAGFMLHGVVHLQPATVAMFGAAMLLLLEN
                     FGTDAESQSQRVHHALTEVEWVTLFFFIGLFIAVYGIEQAGLISAMANGLLSFTGGDL
                     TTTALAILWGSAIASAFVDNIPFVATMIPLIKSMAPVMGGEQAIMPLWWALALGACLG
                     GNGTLVGASANLVVAGIAEQAGAPIRFATFLKIAFPLMLLSIAIAHVYLYLRYLA"
     gene            1467..1793
                     /locus_tag="VFW59_11670"
     CDS             1467..1793
                     /locus_tag="VFW59_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364914.1"
                     /translation="MRKPTLKQRIKAKRRSQSLLVGVTWYNEASWAEVKASATDPECF
                     EDSFEKWKAMAVKARCEFQRSGVRAIECLIDPQDFADWCAQNSLPNNATARAEYVSAV
                     LTAAQG"
     gene            complement(1809..2195)
                     /locus_tag="VFW59_11675"
     CDS             complement(1809..2195)
                     /locus_tag="VFW59_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5364915.1"
                     /translation="MRALIRLFFRTLRLVLNPFMLLWAKLAMPRGVVREAKAQQAVDL
                     ACSKMALYHYPTCPFCIKVLHEMGRLSLPIELRDAQHDATHRETLLQSGGKVQVPCLR
                     ISDVEGQVQWLYESNDIIRYLQQRFA"
BASE COUNT          500 a          761 c          767 g          563 t
ORIGIN      
        1 tgcatctgta tcgcggcgcg aacgacattc ctgtggttga taagccaagt ggccgtttca
       61 tgggcatggt tgcgggctct gacctgctga ctcgcgtctg caaggcaggt tgaacatgca
      121 cgaagaaact caggctatcc atgccatctt tggtttgaat ccgctgtggc tggcgacgtt
      181 catcttcgtc gccacctacg cggtcatcat ctccgagcgc atcaacagag ccatcgtcgc
      241 gctgctgggc ggcgggctga tgattctgtg cggcatcctc acccaggagg cggcgatacg
      301 cggtgtggac ttcaacacgc tgggtctgct gatcggcatg atggtcatcg tctcgatcac
      361 caagaagagc ggggtgttcc agttcgtcgc gatctggatc gccaagcagg tgaaagcaca
      421 ccccgttggc atcatgttga tgatgtcgct ggtcaccgcg ctgttctcgg cgctgctcga
      481 caacgtcacc accgtgctgc tggtcacgcc ggtcatcctg ctgatcaccg aagagctgaa
      541 gatcaagccc tatccctatc tgttcgccat catcttcatg tccaacatcg gcggcacggc
      601 cacgttgatt ggcgacccgc ccaacatcat gatcggctcg gcagccaact tgagcttcaa
      661 cgacttcctg ctgaacctga cgcccattat tccgctgatc gctatcgcga ccctgctgcc
      721 gatctggtgg tggtggcgca aagacatgat cgctgcgccg gaagatcgtg cgcgggtgat
      781 gcgcttcaac gagatcgagg cgatcaccga ctggaagctg ctgaaacagg ccctgctggt
      841 gttctcgctg gtcatcgccg ggtttatgct gcacggcgta gtgcatctgc aaccggccac
      901 ggtcgcgatg ttcggcgcgg cgatgttgct gctgctggag aatttcggca cggatgcgga
      961 atcgcagagc cagcgtgtgc accatgcgct gaccgaggtc gagtgggtca cgctgttctt
     1021 cttcatcggt ctgttcattg ccgtgtacgg catcgagcag gcggggctga tctccgcgat
     1081 ggccaacggc ctgctgagct tcaccggcgg cgacctgacc accaccgcgc tggccatcct
     1141 gtggggctcg gccatcgcct cggccttcgt ggacaacatc cccttcgtcg cgaccatgat
     1201 cccgctgatc aagtcgatgg cacccgtgat gggcggcgaa caggccatca tgccgctgtg
     1261 gtgggcgctg gcgctgggtg cctgtctggg cggcaacggt acgctggtgg gcgcttcagc
     1321 caatctggtt gtggcgggca tcgccgaaca ggccggggcg ccgatccgct tcgccacctt
     1381 cctgaagatc gcctttccgc tgatgttgct gtccatcgcc atcgcgcatg tttatctcta
     1441 tctgcgttat ctggcctgat tttgatatga ggaagccgac actcaagcag cgcatcaagg
     1501 ccaagcgccg cagccagtcg ctgctggtcg gcgtcacctg gtacaacgag gcaagctggg
     1561 ccgaggtgaa ggccagcgcg accgacccgg aatgcttcga ggacagcttc gaaaaatgga
     1621 aggccatggc cgtcaaggcc cgctgcgagt ttcagcgctc cggcgtgcgc gccatcgaat
     1681 gcctgatcga cccgcaggat ttcgcagact ggtgcgcgca gaacagcctg cccaacaatg
     1741 ccactgcccg tgccgaatac gtctcggccg tgctgaccgc agcccaaggc tgacgtcagc
     1801 gccgagactc aggcgaagcg ctgctgcaga tagcggatga tgtcgttcga ctcgtacagc
     1861 cactgcacct gaccttctac atcgctgatg cgcagacagg gcacctgcac cttgccaccc
     1921 gactgcaaca acgtctcgcg atgggtcgca tcatgctgtg catcgcgcag ttcgatgggc
     1981 aaggacaagc gccccatctc gtgcagcacc ttgatgcaga acgggcaggt cgggtagtga
     2041 tacagcgcca tcttgctgca tgccaggtcg accgcctgct gcgctttcgc ttcccgcacc
     2101 acgcccctgg gcatcgccag cttcgcccag agcagcatga acgggttcaa caccagacgc
     2161 aatgtgcgga aaaacagtcg tatcaaggct ctcatggttg tgtgtcctat ggtggggcgt
     2221 gtttcgggaa gcggattgta ctgaagctgg cggctgatag gccttgcgat gcagagggtt
     2281 atcgcgttta atccaagcga aattcaatac cgctgtagtg catttcagaa tgcgatagcg
     2341 ggttttctga tatagacaac gttagagtac acagctatgc attgcggcat aacgaagacg
     2401 attcaaagta aggatgttac gttatgggca aaactggagg gtaatccctt tgatccttgt
     2461 gtcattctgg tggctggggc aaacgcctct cacttgatgt ggccggacga attttgtgag
     2521 cttcttgcat ccaatcaact ctctgtaatt cgctacgatc atagagatac tggaaaatcg
     2581 tccaaattcg a
//
LOCUS       DASUIV010000329         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_272588, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000329 DASUIV010000000
VERSION     DASUIV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272588"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFW59_11680"
     CDS             complement(<1..599)
                     /locus_tag="VFW59_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5364916.1"
                     /translation="MGSTRSMGELKGLDVFRDLSPCGQQMLECGMQLHRFDELKTIIN
                     KGNSVSGAYVVVRGRLRVYSISPGGNEATLYFINPGEVCILALNCIFNNLHYPAWVQA
                     EPDTCVGVIPSLTYHTLFQNEPSIQNLTVHALSVLVFRLMDELEEIHSLNLNERLAKF
                     ILRRTEGCGQLHMTQSELAAHLGTTREVIAKLLRKMVAE"
     gene            complement(622..1995)
                     /locus_tag="VFW59_11685"
     CDS             complement(622..1995)
                     /locus_tag="VFW59_11685"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5364917.1"
                     /translation="MPVVSSALFTFKEDDMGHAGLLGFMNVSLAALVLGCAVLLLMVQ
                     FELGPLSGLSTGRKWAMTLMLGSGMVAFSIKLAIIAAMVHYPGQTIRPHIAASQAPVL
                     EWQHANHSVPPLHANKAYVWRTLPDVAPASAWNPTTPEKVALGERLFHDTALSIDRTV
                     SCSSCHDVRRGAGEDHRRSSLGVGGQVGSRNAPTVWNAAFQSVLFWDGRAASLEEQAK
                     GPPVNPKEMGMSSLEAVAQRVRDDASYREPFALAFGVDQAITIERIAAAIAAYERTLI
                     TADTPYDRFVRGDEKALTPAQLRGMELFQSVGCILCHSGPNFSGASVFDPPPATQRIF
                     PIHDAAKYVDRYRLTRDTGANTPGSSKGVWRIPSLRNVALTAPYFHNGSVDSLPEAVR
                     IMATVQRGRHIGNEGGRTVVWSKQDRTITRSDKAVLSDRDVDDIVAFLKSLSSDALAA
                     RGRAAKP"
     gene            2142..2447
                     /locus_tag="VFW59_11690"
     CDS             2142..2447
                     /locus_tag="VFW59_11690"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX5364918.1"
                     /translation="MKPMRFFLDTHDRARKTFPEKLAREDFEKFYALYEKACYEENVV
                     PIRLHVSYGDGRAFCLNMAEDVEAVRRAHERVGLPYDSITEVATATPGDTFFRRATA"
BASE COUNT          548 a          895 c          876 g          525 t
ORIGIN      
        1 ttttccgcca ccatcttgcg cagcagcttg gcgatcactt cgcgcgtggt gccgaggtgt
       61 gcagccagtt cgctttgcgt catgtgcagt tgcccgcagc cctccgttct tcgcaggatg
      121 aatttggcga ggcgctcgtt caggttcagg gaatggatct cttccagttc gtccatcagc
      181 ctgaacacca gcacagagag tgcatgcacg gtgaggttct ggatggaggg ttcgttctgg
      241 aacagcgtgt ggtaggtgag cgaggggatg acgccgacgc aggtgtcggg ctcggcctgc
      301 acccacgccg ggtagtgcag gttgttgaag atgcagttca gggcgaggat gcagacctcg
      361 ccggggttga tgaaatacag cgtggcctcg ttgccgcccg gcgagatgga atagacgcgc
      421 agcctgccgc gcacgaccac atatgcgccg gagacgctgt ttcccttgtt gatgatggtc
      481 ttcagctcgt cgaaacgatg aagctgcatg ccgcattcga gcatctgctg cccgcagggg
      541 gagaggtcgc ggaacacgtc cagtcctttc aattccccca tcgatctggt actgcccaac
      601 gcacacctcc tgccagattg ctcaaggttt cgcggctctg ccccgcgccg ccagcgcatc
      661 gctgctcagc gacttgagga aagcgacgat gtcgtccaca tcgcggtcgc tcaataccgc
      721 cttgtcgctg cgcgtgatcg ttctatcctg cttcgaccag acgacggtgc ggccgccttc
      781 gttgcctatg tgcctgccgc gctgcacggt cgccatgata cgcaccgcct cgggcaggct
      841 gtcaacactg ccgttgtgga aatagggtgc ggtaagcgcg acgttgcgca gcgaggggat
      901 gcgccagacg cccttgctgc tgcccggcgt gttcgcgccg gtgtcgcggg tgagccggta
      961 gcggtcgacg tacttcgccg catcgtggat cgggaaaatt cgctgcgtgg ccggcggcgg
     1021 atcgaacacg ctggcgccgc tgaagttggg gccggagtgg cacagaatgc agccgactga
     1081 ctggaacaac tccatgccgc gcagttgcgc cggagtcaat gctttctcgt cgcctcgcac
     1141 gaagcggtcg tatggcgtgt cggcagtgat cagcgtgcgc tcataagcgg cgatggcggc
     1201 ggcgatacgc tcgatggtga tggcctggtc cacgccgaat gcgagggcga agggttcgcg
     1261 gtagctggca tcgtcgcgca cccgctgcgc caccgcttcc agcgacgaca tgcccatctc
     1321 cttgggattg accggcggcc ccttcgcctg ctcttccagc gaggcggcgc ggccatccca
     1381 gaacagcacc gactggaacg ccgcgttcca caccgtcggc gcgttgcgcg aaccgacctg
     1441 cccgcccaca cccagcgaac tgcggcggtg gtcctcgccc gcaccgcgcc gcacgtcgtg
     1501 gcaggagctg cacgagaccg tgcggtcgat ggacagggcg gtatcgtgga acaggcgctc
     1561 gcccagcgcc accttctccg gggtcgtcgg gttccaggcc gacgccggtg ccacatcggg
     1621 cagggtgcgc catacgtatg ccttattcgc atggagcggc ggcactgaat gattggcgtg
     1681 ctgccactcc aacaccggag cctgcgatgc ggcaatatgc ggcctgatgg tctgcccggg
     1741 gtaatgaacc atcgcggcga tgatagccag cttgatggag aaggcgacca tgccggagcc
     1801 cagcatcagc gtcatcgccc acttcctgcc cgtcgacaga ccgctcaacg gccccagctc
     1861 gaactgcacc atcagtagca gcaccgcgca gccaaggacc agcgccgcca ggctgacgtt
     1921 cataaatccc agcaatcccg catgtcccat atcgtcctcc ttgaaggtaa acagtgcgga
     1981 cgagactacc ggcaatcgcg cctgccctct gtagcaaaag ctacacaagg cagcagcgca
     2041 aaaaaatcgg aactgcgtaa cttttgctac agatgggact gtcccggatc gctacctttg
     2101 ccgtcgctac accttcgtag cgaacatcac aggagatcaa aatgaaaccg atgcgcttct
     2161 tcctcgacac ccatgaccgc gcccgcaaga cgttcccgga gaaactcgcg cgcgaggatt
     2221 tcgaaaagtt ctatgcactc tatgaaaagg cctgctacga ggagaacgtg gtgccgatcc
     2281 ggctgcacgt aagctacggc gacgggcgtg cgttctgcct gaacatggct gaggacgtag
     2341 aggcagtgcg gcgcgcgcac gagcgggtcg ggctgcccta cgattcgatc accgaagtgg
     2401 cgacggccac gccgggcgac accttcttcc ggcgcgccac tgcctgagct tgatgtagcg
     2461 tcctctgctg cagagggcgc tacatgctcc ggcagagcta gcccttgagc atcgccgcca
     2521 ccgacgcggc catcgacggc ggcacccggc cgatgagctt gctcagttga tggctatcgt
     2581 cgaacagtcc gcctttggaa atgcccatgt cggaatctga aagcaaggtg gagagcgggc
     2641 aacaatcggg gacgtaccac gataattttt aagctgccaa cactcagccg cacggtgcta
     2701 atgataaacg cggcaaggaa tatccgctaa aatatgcaag taacctgact caaggaacaa
     2761 tcgccatgtc catttctttc gccgaagtcg aacagcaagc cacatccctg cttcccgacg
     2821 aacgcgcccg gcttgcggag attc
//
LOCUS       DASUIV010000330         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_180315, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000330 DASUIV010000000
VERSION     DASUIV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180315"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VFW59_11695"
     CDS             <1..1563
                     /locus_tag="VFW59_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEX5364919.1"
                     /translation="SIAPTGTISLAFADNASNGIEPAFSWFYTRKKRMPDGTTKDYQV
                     EDHAWRLYKRLGGDADKLPEAFVTALEISAIDHMKMVAAVAPFIDTSISKTVNVPADY
                     PYEDFKDLYTEAWKAGLKGLATYRPNSVLGSVLSVTPATNSPLSNSPPQAGERADESL
                     RDPYANTEQPQDLVFDQDRRIVLEAAPKPALASLRWPGRPQLLGGSEGWVSQVVKHPL
                     GSFVAFVSHTENGRKVPFEVWVNGSEQPRGLGALAKTLSMDMRALDPVWLRMKLEMLM
                     KTAGDDAFDMPMPPDGEIKRMPSLVSAFAHLLKYRIEQLGALEIDEDATTPMMDALFA
                     KKEPKTGTDGTMSWTVDISNAGTGDDFVLGLKELALPDGQRRPYSMWLSGVYPRALDG
                     LCKLLSLDMRVIDPAWIGMKLRKLLNFGEPLGDFMARVPGGSKMESYPSTVSYVAKLI
                     IHRYAMLGVLDEHGYPVQQMGVLEIPEGQLKVGGHRPLAGKLCKECGNATLIKKDGCE
                     FCTSCGTIGACG"
     gene            complement(1586..3733)
                     /locus_tag="VFW59_11700"
     CDS             complement(1586..3733)
                     /locus_tag="VFW59_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5364920.1"
                     /translation="MLKSDQMQLSQQEKGWLPWLGKTGKFAMRWACFVNRRRYPILEQ
                     TFEGIAANRVNILQGWTDQQWAHLDSLGEDIARHFGDRSVVQDNIDRAHFQDFSELFA
                     VDTEGKVVASSYARRVGRSDLDRRALEQGLKQRFLHGPYIDQNTLAIGPSSSRFHDAV
                     TLMFYLPLRRNNKTLGCLCGRVPNDVLGDLIQREAGHIYHESGDNYLFMVKPVFDPAI
                     QPGTALSRSRFEDNSITVGANLKQGLQTEYGTVQVTNHTEFELRFTDPATRELHPGVR
                     ETIRRGENLFVTYPGYSDYRHIPVVGKGTTFSLPGSPDTWGMMCEADLEEVFRFRSVN
                     FRMMRIYLLVVFSTWLISMVVGHGLSLGMMGTELINLCMLAVGATVFYRFGTNPVVSR
                     MRDMGRMIRSIAEGGGNLSQRVQRGQGIADEPAMVAQWVNSFIDNLDGTVGRVIDGIA
                     EMDTIQGQMAARSQEASHATNLVLEAIGEITASLHKQIADIDIANQTASEIRVAMHDA
                     VEQAKLQFEVVSTRTQGIRDSINESSRTIKTLSDSTAQIGKIVNVINEIADQTNLLAL
                     NAAIEAARAGESGRGFSVVADEVRKLAERTGKATHEISHMIGTVQGQARDAVVTMESG
                     MANMEEGLRLAEVAASDRSGMQDIMERMFTIIQDISNGATSHGKNVQGVAQVTESMRG
                     ALGELDFSATQARQTAHRMKGLADQFQVSQSPA"
BASE COUNT          757 a         1203 c         1124 g          744 t
ORIGIN      
        1 tccatcgcgc cgaccggcac catctcgctg gccttcgccg acaatgcatc gaacggtatc
       61 gagcccgcat tctcgtggtt ctacacgcgc aagaagcgta tgccggacgg caccaccaag
      121 gactatcagg tcgaggacca cgcttggcga ctgtacaaac gcctgggggg cgatgcggac
      181 aaactgccgg aggccttcgt caccgcgctg gagatctccg ccatcgacca catgaagatg
      241 gtcgccgccg tcgcgccttt catcgacact tcgatcagca agaccgtcaa cgtccccgcc
      301 gactatccct atgaagattt caaggacctc tacaccgagg cgtggaaggc aggactgaag
      361 gggctggcga cctaccgtcc aaacagcgtg ctgggttcgg tgctgtcggt cacgcctgca
      421 accaatagtc ccctctccaa ctctcccccg caagcgggag aaagggcaga cgaatcgcta
      481 cgcgatcctt acgccaatac cgagcagccg caggatctgg tgttcgacca ggaccgccgc
      541 atcgtgctgg aggccgcgcc caaacccgca ctcgcctcgc tgcgctggcc gggccgtccc
      601 cagttgcttg gcggcagcga aggctgggtg tcgcaggtgg tgaaacatcc gctcggcagc
      661 ttcgtcgcct tcgtgtcgca caccgagaat ggacgcaagg tgcccttcga ggtgtgggtc
      721 aacggctcgg aacaaccgcg cggtctgggt gcgttggcca agacgctgtc gatggacatg
      781 cgcgcgctcg atcccgtatg gctgcggatg aagcttgaga tgctgatgaa aaccgcaggc
      841 gacgatgcct tcgacatgcc gatgccgccg gacggcgaaa tcaaacgcat gcccagcctg
      901 gtgtcggcct tcgcccatct gctcaaatac cgcatcgaac agctgggcgc actggagatc
      961 gacgaagacg cgaccacgcc gatgatggac gcgctgttcg cgaagaagga gccgaagacc
     1021 ggcaccgacg gcacgatgag ctggacggtg gatatctcca acgccggcac cggcgacgac
     1081 ttcgtgctgg gactgaagga actcgcgttg ccggacggtc agcgccgccc ctattcgatg
     1141 tggctgtccg gcgtttaccc gcgcgcgctc gacggactat gcaagctgct gagcctggac
     1201 atgcgcgtga tcgatccggc ctggataggc atgaagctgc gcaagctgct gaacttcggc
     1261 gaaccgctgg gcgacttcat ggcgcgggtg ccgggcggct ccaagatgga gagctaccct
     1321 tccaccgtgt cctacgtcgc caaactcatc atccaccgct acgcgatgct cggcgtgctc
     1381 gacgaacacg gctacccggt gcagcagatg ggcgtgctgg agatacccga gggacagctc
     1441 aaggtcggcg gccaccgtcc gctggcaggc aagctgtgca aggaatgcgg caatgccacg
     1501 ctgatcaaga aggacggctg cgagttctgc accagttgcg gaacgatagg cgcgtgcggc
     1561 tagttcatta gcccaaaagc agcgatcagg ccggactctg gcttacctga aactggtcgg
     1621 ccagcccctt catgcgatgg gccgtctggc gtgcttgggt tgcgctgaaa tcgagttcgc
     1681 cgagtgcgcc gcgcatagac tcggtgacct gcgccacgcc ttgcacgttc ttgccgtgac
     1741 tggtcgcacc atttgaaata tcctgaatga tggtgaacat gcgctccatg atgtcctgca
     1801 tcccggatcg gtcggaggcg gcgacctcgg caagtctcag cccttcttcc atattcgcca
     1861 taccggactc catggtgaca acggcatcgc gtgcctgccc ctgaacggtg ccgatcatgt
     1921 ggctgatctc atgggtcgcc ttgccggtgc gttcggccag cttgcgcact tcatcggcga
     1981 ccaccgaaaa gcccctgccc gactctccgg cacgggcggc ctcgatggcg gcattcagcg
     2041 ccagcagatt ggtctggtcg gcgatctcgt tgatgacatt gacgatcttg ccgatctggg
     2101 cggtgctgtc gctcagcgtc ttgatcgtgc gcgacgattc attgattgaa tctcgtatcc
     2161 cctgtgtccg ggttgaaacc acctcgaact gaagcttcgc ctgttccacg gcatcgtgca
     2221 tggcaaccct gatctcgctg gcggtctgat tggcaatatc gatatccgca atctgcttgt
     2281 gcagggaagc ggtgatctcc ccgatcgcct ccagcacgag gttggtcgca tggctcgcct
     2341 cctgactccg cgcggccatc tgcccctgta tcgtgtccat ctcggcaatg ccgtcgatga
     2401 cgcgtcccac cgtgccatcc agattgtcga tgaagctgtt gacccattgc gccaccatag
     2461 ccggttcatc ggcgatgccc tgcccgcgct ggacgcgctg ggagagattg ccgccgccct
     2521 cggcgatgct gcgtatcatc ctgcccatgt cgcgcatgcg cgacaccacg ggattggtgc
     2581 cgaagcgata gaacaccgtc gccccgacgg ccagcatgca cagattgatc agctcggttc
     2641 ccatcatacc caacgacagg ccgtggccga caaccatgct gatcagccag gtggaaaaca
     2701 cgaccagcag atagatgcgc atcatccgga aattcacgga acggaagcgg aacacctcct
     2761 cgagatcggc ctcgcacatc atgccccaag tgtccggtga accgggcagg ctgaaggtcg
     2821 tacctttgcc gaccaccggg atatggcgat agtcggaata gccggggtaa gtcacgaaaa
     2881 ggttttctcc ccgcctgatg gtttcgcgca caccgggatg caactccctt gtcgccggat
     2941 cggtaaacct cagttcgaac tcggtatggt tagtcacctg cacggtgccg tactctgtct
     3001 gcagaccctg tttgaggttg gctcccaccg tgatcgagtt gtcttcaaaa cgcgaacggg
     3061 agagcgcggt tcccggctgg atggccggat cgaataccgg cttcaccatg aacagatagt
     3121 tgtctcccga ctcatggtag atgtgcccgg cctcgcgctg gataagatcg cccagcacat
     3181 cgttcggcac cctgccgcac aggcagccca aggtcttgtt attgcgacgc agcggcagat
     3241 agaacatcag cgtcaccgca tcgtgaaaac gcgaagacga agggccgata gccaaggtgt
     3301 tctgatcgat gtaggggccg tgcaggaaac gctgcttcaa cccctgttcg agggcacgcc
     3361 tgtccagatc cgatctgccg actcgtctgg cataggacga agcgacgacc ttgccctctg
     3421 tgtcgaccgc gaacaattcg gagaagtcct gaaaatgcgc gcgatcgatg ttgtcctgaa
     3481 cgacgctccg gtcgccgaaa tgtctggcga tatcttcgcc caggctgtcc agatgcgccc
     3541 actgctggtc ggtccagcct tgcagaatat tgacgcggtt cgccgcaatg ccctcaaagg
     3601 tctgttccag tatcggatag cgccgacgat tcacgaaaca ggcccagcgc atggcgaatt
     3661 taccggtctt tcccaaccag ggcagccagc ctttttcctg ttgggacaat tgcatctgat
     3721 cgctttttag catgttgccg ttccttgtag tgattgcgac aaatgtcgcc atcgccacct
     3781 actaagcaat actcatgcca gcactcaagc cactgatcga taacggcg
//
LOCUS       DASUIV010000331         9424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_644485, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000331 DASUIV010000000
VERSION     DASUIV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644485"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFW59_11705"
     CDS             <1..208
                     /locus_tag="VFW59_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5364921.1"
                     /translation="NVCRADKVTVRALNENGEPFTLEAEGFLAVCIQHEIDHLHGKVF
                     VEYLSQLKQTRLRAKLKKRMRETL"
     gene            216..1142
                     /gene="fmt"
                     /locus_tag="VFW59_11710"
     CDS             216..1142
                     /gene="fmt"
                     /locus_tag="VFW59_11710"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5364922.1"
                     /translation="MKIIFAGTPQFAASALAALLEEHQVVAVLTQPDRPSGRGMQLTA
                     SPVKQLALQHNLPVLQPVSLKTEEAQREIAILNADVMVVAAYGLILPKVVLEMPHYGC
                     LNIHASLLPRWRGAAPIQRAILAGDAETGITIMQMDAGLDTGEMLLRHVCPILPNDTA
                     ATMHDKLAELGGRDILEALRLLQENGLHPVKQDDAAATYAAKLLKSEGQIDWRLDARQ
                     IERAVRAFNPFPVCQATFGEIAVKVWQAKLIDQQGEPGAVLAVDKEGITVACGRGALR
                     LEVLQRPGAKAQPAVQFLQAVQVKVGDNFLVN"
     gene            1143..2417
                     /gene="rsmB"
                     /locus_tag="VFW59_11715"
     CDS             1143..2417
                     /gene="rsmB"
                     /locus_tag="VFW59_11715"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX5364923.1"
                     /translation="MHNIQLAAGQIVQQVLADGRNLNQVLDESLRRKAVWVPAQRAAL
                     QDLSYGTLRFYGQLRALLELLLHKPLSDQRIFYVLLVALYQLQYSKAAQHAVVDHAVR
                     SAQMLNPKVSGLVNAILRNFLRSRATLLEQATRRPEGKYSYQQWWIDELQAQYGERSA
                     SILEAGNQHPPMTLRINRRRGSVDEYAARLAQLDLPARRIAPDALQLERPVAVDKLPG
                     FFDGLVSVQDAGAQYAADLLDVHDGMRVLDACAAPGGKTAHILEGATIEMVAVDKDEL
                     RLQRVAQNLQRLELKAQLLTGDAAAPGAWWDGRPFQRILADVPCSASGVVRRHPDIKW
                     LRRRADIAGFAEQQLAILNALWPLLEPDGKLLYATCSVFQQENEQLVASFLSGQPEAS
                     RLKITLPEKSEGQLLPDDQHDGFFYALLHKAA"
     gene            2377..2982
                     /locus_tag="VFW59_11720"
     CDS             2377..2982
                     /locus_tag="VFW59_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX5364924.1"
                     /translation="MMDFSMRCCTRPLKQLLGFILLVWIGLATAHAQGISVGKAGVRV
                     GDEGTQLVANYDISPTFVVQQALSRGIPLYFSAEFSVTRSRWYWLDEEVFRSEQVTKL
                     SYNVLTRQYRISRGALFQNFATLESALNIMSRQSSVAMPMDALQKDGNYIAAVRLRLD
                     TAQLPRLLQVNALTGKDWTMDSEWYRWVVRPTALGDEGQTE"
     gene            2990..5110
                     /locus_tag="VFW59_11725"
     CDS             2990..5110
                     /locus_tag="VFW59_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364925.1"
                     /translation="MRYLNLVSIVIGGVLLYLLSSSSADTEVFSASYYTLLGLTALFA
                     LALMALVGYQLQRLRTKLREKVFGAKLALRLVFFFTLIAVLPGVLVYVVSVGFLGKSI
                     ESWFDVRVEKALDSGLNLGRSSLDNSLAELSKKTQFISLLLAEKQPGQYQNTLRQLVD
                     EKVAQEAALFTPRGNLVAFATSGTTLTPEMPDADLLHRTLEKGEYSVIDTLPKKGLML
                     RSLIVVNSNVYSGGRYVLQFTQPVPKQIEADAETVQAVYRDYQELTLSRLGLKRLYAI
                     TLTLSLLVVLLTAVSTAFFISERLGSSLEALAEGTRAVAQGDFTGQYPIQSRDELGAL
                     TGLFNQMTQQLAEAKQASEQQQLEVEGARGYLESVLTHLSSGVLALDQDFNLRSANTS
                     AVQILGASLQEMQRLSLAQIADKYSLLASFCHTIIDAFGETSNGEWQRQVERLTKNGT
                     QILLMRGTRLPHGGEAGYVVVFDDISHLLQAERQAAWGEVARRLAHEIKNPLTPIQLS
                     AERLQHKLSDKLDERDALLLQRATQTIVSQVGAMKNMVSDFANYARGPALRLSRLDMH
                     KLVAEVLGLYEANSIPIILSLKAAHSEISGDATRLRQVLHNLLLNAQDALQGQPQPQI
                     RVSTETIGREMLLRVEDSGAGFPQSVLSRAFEPYMTTKAKGTGLGLAIVKKIIEEHGG
                     RIALANSENGGAQISIMLPLIGEV"
     gene            5110..5757
                     /locus_tag="VFW59_11730"
     CDS             5110..5757
                     /locus_tag="VFW59_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364926.1"
                     /translation="MSSKQILVVDDEVGIRELLSEILFDEGYQVHLAENAAQARAYRN
                     EHEPDLVLLDIWMPETDGVALLKEWVEQDLLTMPVVMMSGHGTIETAVEATRIGAVDF
                     LEKPIALQKLLSTIAKAIREGAPKPPILSPEVKAQKIELDVGGQTQQFSLSLDLPLRE
                     ARDQFDAVYFEYHLQKEAGNVSRVAEKVGLERTHLYRKLKQLGVRFAKKGDEESV"
     gene            5895..7913
                     /gene="tkt"
                     /locus_tag="VFW59_11735"
     CDS             5895..7913
                     /gene="tkt"
                     /locus_tag="VFW59_11735"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5364927.1"
                     /translation="MAATRKITPPKFNDITGAIRMLAVDAVQKANSGHPGAPMGMAEI
                     ADVLWNRHLRHNPTNPKWPDRDRFILSNGHGSMLIYALLHLSGYDLPIEELKRFRQMH
                     SKTPGHPEYGYTPGVETTGGPLGQGITNAVGMAMAEKLLAAEFNKPGHEIVNHHTYVF
                     MGDGCMMEGISHEACALAGTWGLGKLTAFWDDNNISIDGHIDGWYTDDTPKRFEAYGW
                     HVIADVNGHDQEEIDAAIKAGKAVTDKPTLICCRTIIGAGSPNKEGTHDVHGAALGDA
                     EVAATREHIGWKYPPFEIPAHVYEAWDARTKGAGLEKLWNNKFAEYKAAFPKEAAEFE
                     RRINNQLPATWAAHAADFIAKTNEKAETIATRKASQNAINGLQPALPEFLGGSADLTG
                     SNLTNWTGAHHVSGKKPGNYISYGVREFGMSHIMNGMALHGGFLPFGGTFLMFSEYAR
                     NALRMSALMKQRVIYVFTHDSIGLGEDGPTHQPVEQTATLRYIPNMETWRPCDTVESA
                     AAWVAAVERNDGPSSLIFSRQNLAFQKRDAAQIANIRKGAYVLSEAAGGKPQAVIIAT
                     GSEVTLAMEAQKALAADGINVRVVSMPSTNVFDKQTQAYKDSVLPAGVKRVAVEAGVT
                     GGWYKYVGLDGAVIGMDCFGESAPAPELFKHFGITTDAVVAAVKKVIG"
     gene            8033..9034
                     /gene="gap"
                     /locus_tag="VFW59_11740"
     CDS             8033..9034
                     /gene="gap"
                     /locus_tag="VFW59_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5364928.1"
                     /translation="MAIKVGINGFGRIGRMAFRAIAKDFPDLEVVAINDLLEPDYLAY
                     MLKYDSVHGNFKGEVAVSGNNLVVNGKTIRLTAERDPANLKWNEAGADLVIECTGFFL
                     TDESCQAHIKAGAKKVVMSAPSKDKTPMFVYGVNHKTYAGQTIVSAASCTTNCLAPVA
                     KVLNDNWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VLPELNGKLTGMAFRVPTSDVSVVDLTVELNKEAKYEDICAAMKAASQTGDMSKTLGY
                     TDEKVVSTDFRGCGFSSIFDAEAGIALDGTFVKVVAWYDNEYGYTCNMLRFVQHIAK"
     gene            9101..>9424
                     /gene="pgk"
                     /locus_tag="VFW59_11745"
     CDS             9101..>9424
                     /gene="pgk"
                     /locus_tag="VFW59_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5364929.1"
                     /translation="MSVIKMTDLDLKGKRVLIRSDLNVPVKDGKVTSDARITASMATI
                     NAALKGGARVMVTSHLGRPEEGVFSEENSLKPVADVIANKLGKPVRLIRDWVDGGFNV
                     ADGELV"
BASE COUNT         2058 a         2364 c         2943 g         2059 t
ORIGIN      
        1 gaatgtatgt cgcgcagaca aggtgaccgt gcgcgcactc aatgaaaatg gtgagccatt
       61 cacgctggag gcggaaggtt ttttggcggt atgtatccag cacgaaatag atcatctgca
      121 tggtaaggtg ttcgtcgaat atctgtcgca actcaagcaa actcgcctcc gcgcaaagct
      181 gaagaagcgt atgcgcgaga cactttaggc ccgccatgaa aattattttt gccggcacgc
      241 cgcagttcgc ggccagcgcg ctggccgcgt tactggaaga gcatcaggtc gtggctgtat
      301 tgacgcagcc ggatcgtcca tcggggcgcg ggatgcagct caccgccagc ccagttaagc
      361 agctagcact gcagcataat ttgccagtgc tgcagcccgt tagcttgaag acagaagagg
      421 cgcagcgtga gattgccatc ctcaatgcgg atgtaatggt ggttgcggcc tacggtttga
      481 tcttgcccaa agtcgtattg gaaatgccgc attatggctg ccttaatatt cacgcttcgc
      541 tattgccgcg ctggcgcggc gcggcaccga tccagcgcgc aatactggcg ggcgatgctg
      601 aaaccggtat aaccattatg cagatggatg caggcttgga tacgggggag atgctgttgc
      661 gacatgtttg cccaatcctg ccgaatgata cggcggcaac gatgcacgat aaattggcgg
      721 aactgggggg gcgcgacatt ctcgaagcgt tgcggctgtt gcaggaaaat ggcctgcacc
      781 cggttaagca ggatgatgcg gcggcgactt acgccgcaaa gctgttaaag agcgaggggc
      841 agatcgactg gcgactggat gctcggcaga ttgagcgggc ggtacgggcc ttcaatccct
      901 ttcccgtgtg tcaggcgacc tttggtgaga tcgcggtaaa ggtgtggcag gcgaagctca
      961 ttgatcagca gggggagccg ggtgcggtgc tcgcagtaga caaggaagga attacggtcg
     1021 catgcgggag gggcgcattg cgtctggaag tgttgcagcg tcctggtgcg aaggcgcagc
     1081 ctgccgtgca gttcctgcaa gccgtgcagg tgaaggtcgg agacaatttc ttggtaaatt
     1141 aaatgcacaa tatacaactc gccgcaggcc aaattgttca gcaagtactg gcggatggac
     1201 gaaaccttaa ccaggtgctg gatgagtcat tgcgcagaaa agcagtatgg gtgccggcgc
     1261 agcgcgctgc tctgcaggat ttgagttatg gcacgctgcg tttttatggc cagttgcgcg
     1321 ctctattaga gctgttgctg cataagccgc tatcggatca gcgcattttt tatgtgctgc
     1381 tggtggcgct gtatcaactg caatacagca aggcggcgca gcatgcggtg gtggatcatg
     1441 cggtgcgatc cgcacaaatg ctgaatccga aagtgagcgg tttggtgaat gcaattctgc
     1501 gcaattttct gcgcagtcgg gcgactttgc tggaacaggc gacacggcgc ccggaaggga
     1561 aatacagcta tcagcaatgg tggatcgatg agttgcaggc gcagtatggg gagcgcagtg
     1621 cgtcgatctt ggaggccggc aaccagcatc cgccgatgac tttgcgcatc aaccgccggc
     1681 gcggctcggt agacgaatat gcagcgcgat tggcgcaact ggatttgccc gcgcggcgca
     1741 ttgcacccga tgcgctgcaa ttggaacggc ctgttgcggt agataagctc ccggggtttt
     1801 ttgatgggct ggtatcggta caagatgccg gagcgcagta tgcggcggac ttgctggatg
     1861 ttcatgatgg gatgcgtgtg ctggatgctt gtgctgcgcc gggcggcaag accgcgcata
     1921 ttctggaagg tgcaaccatc gaaatggttg cggtggacaa agatgagctg cgtttgcagc
     1981 gcgtcgcgca gaatttgcaa cggctggagc taaaggcgca gttgctgacg ggtgatgcgg
     2041 ctgcgccggg agcttggtgg gatggcaggc catttcagcg tatccttgcc gatgtgccct
     2101 gctctgcgtc aggcgtggtg cgtcgccatc cggatatcaa atggttgagg cgtcgtgccg
     2161 atatcgccgg atttgccgaa cagcagcttg cgattctaaa cgcgttgtgg ccgctgttgg
     2221 agcctgatgg taaattgctc tatgcgacct gttcggtgtt ccagcaggaa aatgagcagc
     2281 ttgtcgcctc ctttctctcg ggacagccgg aggcgagccg attgaaaata accctgccag
     2341 aaaagagcga agggcaacta ttacccgatg accagcatga tggatttttc tatgcgttgc
     2401 tgcacaaggc cgcttaagca actgctgggc ttcatcctgc tggtttggat cgggctcgca
     2461 acggcacatg cacagggtat ctcagtgggg aaggcgggcg tgcgcgtggg tgatgaaggc
     2521 actcagttgg tcgcgaacta tgacatcagc cctacttttg tcgtgcagca ggcactgtcg
     2581 cgcggcatcc cgttatattt ttccgctgaa ttctcggtga cccggtcgcg ctggtattgg
     2641 ctggatgaag aggtctttcg cagcgaacaa gtcaccaagc tttcttacaa tgtgttgacg
     2701 cggcaatacc gcatttcgcg cggtgcgctg ttccagaatt ttgcgacgct ggaaagtgcg
     2761 ttgaatatca tgtctcgtca gagttcggtg gcgatgccca tggatgcgtt gcagaaggac
     2821 gggaattata ttgcggcggt tcgactgcgg ttggacacgg cgcagttacc tagactgttg
     2881 caagtgaatg cgttgaccgg caaggattgg acaatggatt cggaatggta tcgctgggtg
     2941 gtgcgtccga cagcgctggg tgatgagggg cagacggagt gaccgagcga tgaggtactt
     3001 gaatctcgtc agcatcgtga tcggtggcgt attgctgtat ctgctgtcca gtagcagtgc
     3061 tgataccgag gtgttctcgg ccagttatta cacgctgctc gggctgacgg cattgttcgc
     3121 gctggctttg atggcgctgg tcggttatca gcttcagcgc ctgcgtacca agctcagaga
     3181 aaaggtattt ggagccaagc ttgcattgcg gctggtgttc ttctttacac tgattgccgt
     3241 attgccgggc gtgttggtgt atgtcgtgtc ggtgggtttt ctgggcaaga gcatcgagtc
     3301 ctggttcgat gtgcgtgtcg agaaggcgct ggattcgggt ctcaatctgg ggcgaagcag
     3361 tctcgataac agcttggccg aattgagtaa aaaaacccag ttcatttcgt tgttgctggc
     3421 ggagaagcag ccaggccagt atcaaaacac cctgaggcag ctggtggatg agaaagtcgc
     3481 acaggaggcg gcgctgttta cccccagagg gaatttggtc gcatttgcga cctctggtac
     3541 gacgctgacg ccagagatgc ccgatgcgga cttgttgcat cggacgctgg aaaagggcga
     3601 gtactcggta atcgatacct tgccgaagaa ggggttgatg ttgcgctccc tgatcgtggt
     3661 gaactcaaat gtatattccg gcggacgtta tgtgttgcag ttcacccagc ccgtgccgaa
     3721 acagatcgag gcagatgccg agaccgttca ggcggtatat cgcgattatc aagagttgac
     3781 actttcgcgg cttggattaa agcgcttgta tgcgataacg ctgacgttgt cattattggt
     3841 tgtgttgttg acggcagtgt cgacagcctt ctttatcagt gagcggctgg gctcctcgtt
     3901 ggaggcgttg gcggaaggaa cgcgcgcggt cgcacagggt gattttactg gccaatatcc
     3961 gatacagagt cgcgatgagt tgggtgcctt gacgggtctg ttcaaccaga tgactcagca
     4021 attggccgag gcaaaacagg ctagtgaaca acagcaactc gaggttgagg gggcgcgagg
     4081 ctatctggag agcgtgctga cccatttatc atcaggcgtg ttggcgctgg atcaggactt
     4141 taatctgcgc tcggcaaata ccagtgccgt gcagatattg ggagcttcct tgcaggagat
     4201 gcagcgtctg tcactagccc agatcgccga caaatacagc ctgctggcat cgttttgcca
     4261 caccatcatc gatgccttcg gtgaaaccag caatggcgag tggcaacgac aagtagagcg
     4321 tctgaccaag aatggcactc agatcttgtt gatgcgtggt acgcgtctgc cgcatggcgg
     4381 ggaagctggt tacgtggtgg tgtttgacga catcagtcac ttgttgcagg ccgagcgtca
     4441 ggcggcatgg ggagaggtcg cgcgacgact ggcgcatgag atcaagaatc cactgacgcc
     4501 aatccagttg tcagcggagc gattgcagca caagcttagc gataagctcg atgagcgcga
     4561 tgcgttgttg ttgcagcgtg ccacacagac tatcgtcagt caggttgggg caatgaaaaa
     4621 catggtcagt gactttgcca attacgcgcg cggcccggca ttgcgcttat cgcgtttgga
     4681 tatgcacaaa ctcgttgcgg aggtgctggg gttgtatgag gcgaacagca tacccatcat
     4741 tctgagcctc aaggccgcgc atagcgagat cagcggagat gcgacgcgcc tgcgtcaggt
     4801 tctccacaat ttgttgctga atgcgcagga tgccttgcag gggcagccgc aaccacagat
     4861 cagggtgtct acagaaacaa taggccgcga gatgttgctg cgggtcgagg acagcggcgc
     4921 aggatttcca cagagcgtgt tgtcgcgggc atttgagccg tacatgacca ccaaggcaaa
     4981 gggcacggga ttgggattgg cgatcgtgaa gaagattatt gaagagcacg gcgggcgcat
     5041 tgctctagcc aacagtgaga acggcggagc gcagatcagc atcatgctgc cgctgattgg
     5101 ggaggtttga tgtcaagtaa gcagattttg gtggtggatg acgaagtcgg catccgtgaa
     5161 ttgttgtcgg agatattgtt cgatgagggt tatcaggtgc atctggctga gaatgcggct
     5221 caggcccggg cctatcgcaa cgagcatgag ccggatctgg tgttgctgga tatctggatg
     5281 ccagaaaccg atggcgtggc gttgctcaag gaatgggttg agcaggattt gttaacgatg
     5341 ccggtggtga tgatgtcagg gcacggcacc atagaaacgg ccgtcgaggc gacgcgcatc
     5401 ggtgcagtgg attttctgga gaagcccatt gcgttgcaga agctgctgag tacgatagca
     5461 aaagccattc gggaaggcgc gcccaagccg ccgatcctga gcccggaagt taaggcgcaa
     5521 aaaattgagc tggatgttgg cgggcaaaca cagcagttct ccttgtcatt ggacttgcca
     5581 ttgcgcgagg cgcgcgacca gttcgatgcg gtctactttg agtatcatct gcaaaaagag
     5641 gccggtaacg tttcgagggt ggccgagaag gtcggcttgg aacgcaccca tttgtaccga
     5701 aaattgaagc aattgggtgt tcgattcgcc aagaagggcg acgaagaaag cgtttaaggc
     5761 aaggcttaaa ctccggatgc gggcggcggt tgcccaaatc cgggggctgc ggcataatta
     5821 ccgatccaga attaatactt ataaccaatc caaccagtaa cccatcccac aaggatgttt
     5881 aggagacaat agcaatggct gcgacacgca agattacccc accaaaattc aacgacatca
     5941 ccggcgcgat tcgtatgctg gcagtcgatg ccgtgcagaa agccaactcc gggcatccag
     6001 gcgcgccgat gggcatggca gagatcgccg atgtgctgtg gaaccgccat ctgcgccaca
     6061 acccaaccaa tcccaagtgg cctgatcgcg atcgtttcat cctgtccaac ggacacggtt
     6121 cgatgttgat ctacgccttg ctgcacctgt ctggttatga tctgccgatc gaagagctga
     6181 agcgcttccg tcagatgcac tccaagactc ccggtcaccc ggagtacggt tatacgcccg
     6241 gcgttgaaac gactggcggc ccgctcggtc agggcatcac caatgctgta ggcatggcga
     6301 tggcggaaaa gttgctggct gccgagttca acaagccggg tcacgagatc gtcaatcacc
     6361 atacctatgt gtttatgggc gacggttgca tgatggaagg catttcgcac gaagcttgcg
     6421 cgctggctgg cacttggggg ctgggcaagc tgacggcatt ctgggatgac aacaacatct
     6481 ccatcgacgg tcacattgat ggctggtata ccgacgatac gcctaagcgt ttcgaagcct
     6541 acggctggca tgtgattgct gatgtgaatg gccacgatca ggaagagatc gatgccgcga
     6601 tcaaggcggg caaggctgtc accgacaagc cgacgctgat ctgctgccgc accattatcg
     6661 gcgcgggctc ccctaacaag gaaggtacgc acgacgtgca cggcgctgcg ctcggcgatg
     6721 ccgaagttgc cgcgactcgc gagcacatcg gctggaaata tcctccgttt gaaatcccgg
     6781 ctcatgtgta tgaggcatgg gatgcgcgca ccaagggtgc cggcctggaa aaattgtgga
     6841 acaacaagtt cgccgaatac aaggctgcgt tcccgaaaga ggccgccgag ttcgagcgtc
     6901 gtatcaataa tcagctaccg gctacttggg ctgctcacgc cgccgatttc atcgccaaga
     6961 ccaacgagaa agccgaaact atcgctactc gcaaggcttc gcagaatgcc atcaacggtt
     7021 tgcagccagc attgccagag ttcctgggcg gctctgccga cctgacaggc tctaatctga
     7081 ccaactggac cggtgctcat catgtttccg gcaagaagcc aggtaactac atcagctacg
     7141 gtgtgcgtga gttcggtatg tcgcacatca tgaacggcat ggcattgcac ggcggcttcc
     7201 tgccattcgg cggaaccttc ctgatgttct ccgaatatgc gcgtaatgca ttgcgcatgt
     7261 ccgcactgat gaagcagcgc gtgatctatg tgtttaccca cgactccatc ggtctgggtg
     7321 aagatggtcc gactcaccag ccggtcgagc agacagcgac actgcgctac attcctaata
     7381 tggaaacatg gcgtccatgc gacacggttg agtccgctgc cgcatgggtg gctgccgtag
     7441 agcgtaacga tggcccatcc agtctgattt tcagccgcca gaatctggcg ttccagaagc
     7501 gcgatgcagc gcaaatcgcc aatatccgta aaggcgctta tgtgttgtcc gaagctgccg
     7561 gcggcaagcc acaggcggta atcatcgcga ccggctccga agtgacgctg gcgatggagg
     7621 cgcagaaggc cttggctgct gacggtatca acgtgcgcgt ggtgtcgatg ccgtcgacca
     7681 atgtgttcga caagcagact caggcctata aagacagcgt gctgccagca ggcgtgaagc
     7741 gcgtcgccgt tgaggcgggc gtgactggcg gctggtacaa atatgtgggt ctggatggcg
     7801 cggtgatcgg tatggattgc ttcggtgaat ccgctccggc tccagagttg ttcaagcact
     7861 ttggcatcac tacggacgcc gtggtggcgg cggtgaaaaa ggttattggt taagcattga
     7921 ggtacggccg cattgcagaa atatttgagg acgggcgtcc ccatccgatc tgcaatgcgg
     7981 tctagggtgt gcggtaaaca taaagtttct aattactaaa cgggagaaaa caatggctat
     8041 caaagttggt atcaatggtt ttggtcgtat cggccgcatg gcattccgcg caatcgcgaa
     8101 ggactttccg gatctcgaag tggtcgcgat caacgacctg ctggagccgg actatctggc
     8161 ttacatgctg aagtacgact ccgtgcacgg caatttcaag ggtgaagtcg cggtgagcgg
     8221 caacaacttg gtcgtgaacg gtaagaccat ccgtctgacc gccgagcgcg accccgctaa
     8281 cctgaagtgg aacgaagctg gtgccgatct cgtgatcgag tgtaccggtt tcttcctgac
     8341 cgatgaatcc tgtcaggctc acatcaaggc tggtgccaag aaggtcgtga tgtccgcacc
     8401 gtccaaggac aagactccga tgttcgtgta cggcgtgaac cacaagacct acgccggcca
     8461 gaccattgtc tccgccgctt cctgcaccac taactgcctg gcgcctgtcg ccaaggtgtt
     8521 gaacgataac tggggcatca agcgcggcct gatgacgact gtgcatgctg ctactgcgac
     8581 gcagaagacc gttgacggcc cgtccaacaa ggactggcgc ggtggtcgcg gcattctgga
     8641 aaacatcatc ccgtcctcta ccggtgctgc caaggccgtg ggcgtagtgt tgccggaact
     8701 gaacggcaag ctgaccggta tggcattccg cgtaccgact tctgatgtct ccgtggtcga
     8761 cctgaccgtc gagctgaaca aggaagccaa gtacgaagac atctgcgcag cgatgaaggc
     8821 tgcttcgcag accggcgaca tgagcaagac gttgggttac accgatgaga aggtggtttc
     8881 caccgacttc cgcggctgcg gcttctcgtc gatcttcgac gccgaagctg gtatcgcgct
     8941 ggacggcacc ttcgtcaagg tggtggcttg gtacgacaat gagtacggtt acacctgcaa
     9001 catgctgcgc ttcgtgcagc acatcgccaa gtaatacgtt ctgcaaaggg ctggcttcgc
     9061 cggccctttt agctttcgag tctgtttcaa aggaaaaatc atgtccgtta ttaaaatgac
     9121 cgatctggat ctcaagggca aacgcgtcct gatccgttcc gatctcaatg tgcccgtcaa
     9181 agacggcaaa gtaacctccg atgcgcgcat caccgcctcg atggcaacca tcaatgccgc
     9241 tctgaaaggt ggagcgcgcg tgatggtgac ttcccatctg ggccgtcccg aagaaggcgt
     9301 cttctccgaa gagaactcgc tcaagccggt ggccgatgta atcgccaaca agctgggtaa
     9361 gccagtgcgt ctgatccgtg attgggtgga tggcggtttc aatgtcgctg acggtgagct
     9421 ggtg
//
LOCUS       DASUIV010000332        10389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_274273, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000332 DASUIV010000000
VERSION     DASUIV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274273"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..118
                     /locus_tag="VFW59_11750"
     tRNA            43..118
                     /locus_tag="VFW59_11750"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Gly,seq:gcc)
     gene            162..237
                     /locus_tag="VFW59_11755"
     tRNA            162..237
                     /locus_tag="VFW59_11755"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:196..198,aa:Glu,seq:ttc)
     gene            348..423
                     /locus_tag="VFW59_11760"
     tRNA            348..423
                     /locus_tag="VFW59_11760"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:381..383,aa:Gly,seq:gcc)
     gene            851..2644
                     /locus_tag="VFW59_11765"
     CDS             851..2644
                     /locus_tag="VFW59_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5364930.1"
                     /translation="MVTGGNADLSPILESHARDPGALLQILREIQELRGCITPATVSY
                     LCDQLDVPRTRIEGVAGFYSFLYQQPHGEYRVLFSDNITDRMLGNQSLLEAMCHQLWL
                     QPGKVSEDGLVSIGTTSCTGMCDQGPAMLVNGRAVTRLTLQRVQEIGQLIRNRTPLDE
                     WPADFFRVDDNIRRSGPLLENSLVPGSLLRTALSMKSAGIVEEIKNSGLRGRGGAGFP
                     TGLKWDACRNAAGTTKHVVCNADEGEPGTFKDRVLLTRQADLVFEGMTLCALAIGAQH
                     GYLYLRGEYRYLFEPLQAVLQRRRQNNLLGANILGQAGFDFDIDIHLGAGAYICGEES
                     ALIESLEGKRGVPRNRPPFPVTHGYKQQPTAVDNVETFCSAVLAIQMGAENFRSIGTP
                     KSSGSKLISVSGDCPRPGIYEYPFGVSVREVLEDCGAVNTRAVQISGPSGVCVAETEF
                     DRKLGFEDLPTAGAFMVFDDSRDMFEVARNFVHFFAHESCGFCTPCRVGTSMLKQTMD
                     KIAEGHGTRYDLAEIENLDHVLQTTSHCGLGRTACNPVLNTLKHFRPAYENRLKSLDF
                     EPAFDLDGALARARQMTGRDDAGAHLKTEAT"
     gene            2641..3393
                     /locus_tag="VFW59_11770"
     CDS             2641..3393
                     /locus_tag="VFW59_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5364931.1"
                     /translation="MSDTFHLDGSDVPFEPGQTVMQAALAAGHYIPHLCYHPEFKPHG
                     SCKLCTVKVNGRTSASCTMPAEAGLEIESDTEELNALRRTLVQMLFAEGNHFCPSCEK
                     SGNCMLQALGYDLEVHTAGFRHLYPDRPLDATHPDILLDFNRCILCELCVRASSEVDG
                     KNVFGLSGRGLTKHLIVNAESGLLKDTDIELDDKAMHVCPVGVILPKRVGFSIPIGQR
                     RYDQRPISAQALDDAPRQSSAGDGCAACEGTP"
     gene            3390..3929
                     /locus_tag="VFW59_11775"
     CDS             3390..3929
                     /locus_tag="VFW59_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HEX5364932.1"
                     /translation="MSKKLRIATTSLAGCFGCHMSFLDIDERILTLLELVEFDRSPIT
                     DIKHCSPCDIGLIEGGICNAENVHVLREFRKNCKILIAIGACAINGGLPAQRNHLDLG
                     SCLTEVYLTEPGLQHGHIPNDPELPLPLDKVHPLHEVVKIDYFIPGCPPSGDAIWKFL
                     TDLIAGKTPELGHGLIKYD"
     gene            3922..5436
                     /locus_tag="VFW59_11780"
     CDS             3922..5436
                     /locus_tag="VFW59_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX5364933.1"
                     /translation="MTKLTTKNTGADNLETAAHPENLRRVAIDPVSRVEGHGKVTLLL
                     DENDRVHQVRLHIVEFRGFEKFIQGRPYWEAPVMVQRLCGICPVSHHLAASKALDMIV
                     GATLTPTAEKVRRLMHYGQILQSHALHFFHLCSPDLLFGFGSDVAKRNIVGIVAAYPE
                     IAKQGVFLRKYGQEVIRVTAGKRVHGTGSIPGGVNKNVSIEERDYLLKDIDQMVEWSR
                     DAVGLIRKLFEQNLDAYNSFGRFKAHTLNLVRPDGAMDLYHGGLRARDMDGNTLFDHY
                     DYSHYWDVIFEDVKPWSYMKFPFLRSLGPEEGWYRVGPLSRVTQCDFIPSPLAEQARR
                     EFMAFDGGSVTGATLGFHWARMIEMLHAAEMIKELLFDTDLQGDELVNIGERQARGVG
                     VIEAPRGTLIHHYKVDENDQIIRCNLIVSTTHNNQAMNEAIRQVARQYIDGRKLTEGL
                     LNHIEVAIRAFDPCLSCATHALGKMPLEVELLDVSGDRVDMLTRSSDGTFCEAC"
     gene            5436..5939
                     /locus_tag="VFW59_11785"
     CDS             5436..5939
                     /locus_tag="VFW59_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX5364934.1"
                     /translation="MTMPVLVFAIGNESRGDDALAPLLLRDLDEWAKACRLDEQVELI
                     EDYQLQVEHVTDLVGRSAVLFVDADMSCAEPFHFSEIAAGHDSSYTSHAMTPFALLHA
                     YRHVYGKDAPPSFLLRVRGYGFELGDALSGEAAKNLEAATGLAGELCCEHDLQRWRQH
                     VAMHDIA"
     gene            5956..6537
                     /locus_tag="VFW59_11790"
     CDS             5956..6537
                     /locus_tag="VFW59_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX5364935.1"
                     /translation="MFESRIHGRGGQGVVTAAEMLSIAAFLEGRHAQAFPSFGSERMG
                     APVVAFCRIADKEIRLREPIMEPDAILIQDPTLLHQVDVFSGLKPDGYILLNTNHSFE
                     ALGLEEFIRGRPAERLCTIPATEIAIKHIGRPIPNVPLIAGFAALSGLIRLESVVKAI
                     GEKFSGKVAAGNIAAATEAYNLVKAMREESTHA"
     gene            6530..7783
                     /gene="porA"
                     /locus_tag="VFW59_11795"
     CDS             6530..7783
                     /gene="porA"
                     /locus_tag="VFW59_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEX5364936.1"
                     /translation="MLKQTEGSKAVAEAIALCRPEVICAYPISPQTHIVEALGESVKS
                     GELTSCEFINVESEFAAMSVAIGASAAGARAYTATASQGLLFMVEAVYNASGLGLPIV
                     MTVANRAIGAPINIWNDHSDSLSQRDSGWMQLFAETNQEALDLHIQAFRLAEELSLPV
                     MVCMDGFILTHAYERVDMPTQQQVDAFLPPYEPRQVLDPREPVSIGAMVGPEAFMEVR
                     YLAHAKQMQALERIPQLVAEFKQIFGRDSGGLTHTYRAEDAETMVVAMGSVLGTIKDT
                     IDEMRNDGHKIGVLGITSYRPFPLAQVRDALQNAKRVVVLEKSLAVGIGGILSTDVRM
                     ALSGIQLHGYTVVAGLGGRAITRKSLHKLFREAEQDALQPLTFLDLDWNVVNKQLERE
                     RAVHRSGPAAESILRDIGTVASKII"
     gene            7793..8797
                     /locus_tag="VFW59_11800"
     CDS             7793..8797
                     /locus_tag="VFW59_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5364937.1"
                     /translation="MSFQPVKFYQTGTFTVGNRLLHADERSVQSHQDRSNSINSGHRA
                     CQGCGEALGARYALDAAMEACDSQLIAANATGCLEVFSTPYPETSWQIPWMHSLFGNA
                     AAVATGIAAAMKVKGRNEVRVVAQGGDGGTTDIGFGCLSGMFERNDDVLYICYDNEAY
                     MNTGVQRSSATPAAARTATTMPVGPNPGNVFGQGKFVPAIAMAHEIPYVATATVADLR
                     DLEAKVKRAMEFRGARYIHILVPCPLGWGTASHDTIRMARLAHETGLFPVFEAEHGVQ
                     VSALPIRKQVPVEDYLKPQKRFAHLFGANPKLEVIARIQARADRNIRKYGLVPQQEVL
                     "
     gene            8797..>10389
                     /locus_tag="VFW59_11805"
     CDS             8797..>10389
                     /locus_tag="VFW59_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX5364938.1"
                     /translation="MDKPFAITLDPGSSLANHTGSWRTKRPVYLDRLPPCNATCPAGE
                     NIQGWLFHAEAGNYEQAWRVLVENNPLPAIMGRVCYHPCEGACNRGQLDSSVGINSVE
                     RFLGDEAIKRGWQFDAPAAASGKKVLVVGAGPSGLSAAYHLARMGHLVEIHEAGPLSG
                     GMMRFGIPKYRLPREVLDAEVARILKLGVTLKLDTKVENIEQSMKRGGFDAAFLAVGA
                     HIGKRTYIPAGDAAKILDAVSVLHNMEGEDRPLLGRRVVVYGGGNTAIDVARTAKRMG
                     AAEAIIVYRRTREKMPAHDFEVEEALQEGVMIKWLSTIKQADAHSLVVERMELDDKGF
                     PQPTGEFETMEADSLVLALGQDVDLSLLDGVSGLEIKDGTVQVGINMMTGHDGIFAGG
                     DMVPSERTVTVAVGHGKKAAHHIDAWLNGRSLEAETKHEIATFDKLNTWYYSDAPKTV
                     RPVLDIIRRQTGFDEVQGGLDEKNALFEARRCLSCGNCFECDNCYGVCPDNAVIKLGP
                     GNGYAINLDYCKGCGICVAECPC"
BASE COUNT         2134 a         2838 c         3175 g         2242 t
ORIGIN      
        1 tactcacaaa ggagccggag gcgatccggc ttttttattt tcgcgggagt agctcagttg
       61 gtagagcgat accttgccaa ggtataggtc gagagttcga gcctcttctc ccgctccagt
      121 attaaagaaa ggccggataa attccggcct ttcttttaaa agtccccttc gtctagaggc
      181 ctaggacacc gccctttcac ggcgataaca cgagttcgaa tctcgtaggg gacgccagat
      241 taagtagcag gttggttttt tcccagtctg tcatgagtgt gcgattcagc agcaatgttg
      301 agcgttactc acaaaggagc cggaggcgat ccggcttttt tattttcgcg ggagtagctc
      361 agttggtaga gcgatacctt gccaaggtat aggtcgagag ttcgagcctc ttctcccgct
      421 ccagcatcaa agaaaggccg gataacatcc ggcctttttc tttttccaca aagcggccat
      481 cccagccgac cttaaacgcc cccaaggcgt agcgcgtttt gagaaataga atggctgcgt
      541 ttggttcttc gccgcacaga acttatcggc aagcaggcgg atactcgact cggtaaagat
      601 ctgcagacaa ggctattcgt ttgaaatttc agtcatgcag tttggccatg accagcagat
      661 aaacgaataa agaacatttt tggcacacca tcctcatcgc ggttgagcag tgttcagaaa
      721 tgagtcagga tttagaccga tgaaatcagc gtcccctgaa ttgctatggg ataggggggc
      781 gcatacaatc aggcggtggt aatggcgatg ctgtccgtgg cgttattgat gcaagctcta
      841 gggaggtttc atggtgacag gtgggaatgc cgatttatct ccgatattgg aaagccatgc
      901 ccgtgatccg ggagctttgt tgcagatatt gcgcgaaatt caggagttgc gcggctgcat
      961 tactccggcc acggtctctt atctctgcga ccagcttgat gttcctcgca cccgtatcga
     1021 aggtgtcgct ggcttctatt cttttttata tcagcagccg cacggcgagt atcgtgtgct
     1081 gttctcggac aatatcactg accgcatgct gggcaaccaa tcgctgctgg aggcgatgtg
     1141 tcatcagctc tggttgcagc cgggcaaggt ttccgaagac ggactggtca gtatcggcac
     1201 aacttcctgc accggcatgt gcgatcaggg cccggccatg ctggtcaatg gtcgcgctgt
     1261 tacccggtta actctgcagc gcgtgcagga aatcgggcag ctgatccgaa accgcacgcc
     1321 actggatgaa tggccggccg atttcttccg tgtggatgac aatatccgcc ggagcggccc
     1381 attgctggaa aactcccttg tcccaggatc gctattgcgt accgcactga gtatgaagag
     1441 tgcgggtatc gtcgaagaga ttaaaaactc cggtttgcgt gggcgcggtg gagctgggtt
     1501 tcctactggg ctgaagtggg atgcatgtcg gaatgcggct ggtaccacca agcatgtggt
     1561 atgtaacgcc gatgaaggcg agcctggcac ctttaaggat cgcgtattgc tcacccgtca
     1621 ggccgatctg gtgttcgagg gcatgacgct gtgtgccctg gccatcggcg ctcaacatgg
     1681 ttatctatat ttgcgggggg aatatcgtta tctgtttgag ccgttgcaag cggtactgca
     1741 acgccgcaga caaaacaatc tgttgggtgc gaatatcctc ggtcaggctg gttttgattt
     1801 tgacatcgac attcatctgg gggctggagc gtacatctgc ggcgaagaat ctgcgctgat
     1861 cgagtcgctg gaaggcaagc ggggcgttcc gcgcaaccgc ccgccgttcc cggtgaccca
     1921 tggctacaaa cagcaaccga ccgcagtgga taatgttgag actttttgca gtgcggtgct
     1981 ggcgatacag atgggggcgg agaacttccg ttccatcggt acgccaaaat catccggcag
     2041 caaactgatt tctgtatccg gcgattgccc gcgtcccgga atttatgaat atccgttcgg
     2101 agtgtcggtg cgcgaggtat tggaggattg tggagcggtg aatacgcgcg cagtccagat
     2161 tagcggccct tccggggtgt gtgtcgccga aacggaattc gaccgcaagc tgggattcga
     2221 ggatctgccg actgcgggag cgttcatggt gttcgacgat agtcgcgaca tgtttgaagt
     2281 ggcacgcaac ttcgtgcatt tcttcgcaca tgagagttgt ggcttctgca cgccgtgccg
     2341 cgtcggcacc tcgatgttga agcagacgat ggacaagatt gcagaggggc acggcacgcg
     2401 ttacgacctc gctgagatag aaaatctgga tcacgtattg cagaccacct cgcactgcgg
     2461 actgggacga accgcctgca atcctgtgtt gaacaccctc aaacatttcc gtcctgccta
     2521 tgaaaaccgt ctgaaatcac tggattttga accagctttc gatctggatg gggcactggc
     2581 gcgtgcgcgc cagatgaccg gacgcgatga tgcgggtgcc cacctaaaaa cggaggcaac
     2641 atgagcgata cctttcatct tgacggttct gatgttccgt ttgagcccgg tcagacggtg
     2701 atgcaggccg cattggctgc cgggcattac attcctcacc tgtgctatca cccggaattc
     2761 aagccgcacg gcagttgcaa actatgcacg gtcaaggtca atggtcggac ctcggcctcc
     2821 tgcaccatgc cagccgaggc tgggctggag atcgagagcg acactgaaga gctgaacgcg
     2881 ctacgccgca ctctggtgca gatgctattc gctgaaggga accatttctg tccatcttgc
     2941 gagaagagtg gcaattgcat gctgcaagcg ctgggctacg atctcgaagt gcatactgcc
     3001 ggttttcgcc acctttatcc ggatcgtccg ctggatgcca cgcatcccga cattctgctg
     3061 gatttcaatc gctgtatatt gtgtgagctc tgtgtgcgtg cctccagcga agtcgatggc
     3121 aagaacgtgt tcggcctgtc tggacggggg ttgaccaagc atctgatcgt caatgccgaa
     3181 tctggcctgc tgaaagatac tgatatcgag ctggacgaca aggcgatgca tgtctgcccg
     3241 gtgggcgtga ttctgcccaa gcgggtcggt ttcagtatcc ccattggcca acgtcgctat
     3301 gaccagcgtc cgatcagtgc tcaggcactg gatgacgcac ctcggcaatc gagtgccggc
     3361 gatggatgtg cagcttgcga gggaacgcca tgagcaaaaa gttacgtatt gcgaccactt
     3421 cgttagccgg ttgtttcggc tgtcatatgt ccttcctcga tattgacgag cgcattctga
     3481 cgctgctcga actggtcgaa ttcgaccgtt cgccgattac tgacatcaag cattgctcgc
     3541 cctgtgatat cggcttgatc gaaggcggaa tctgcaatgc ggagaatgtg catgtactcc
     3601 gcgagttcag aaaaaactgc aaaatcctga tcgccatcgg tgcctgtgct atcaacggag
     3661 gtctgccggc ccagcgaaat catctcgatc tgggcagttg cttgaccgag gtatatctga
     3721 ccgagccggg cttgcagcat ggtcacatcc ccaacgatcc ggaactgccg ctgccgctgg
     3781 acaaggttca tccactgcat gaggttgtaa agatcgacta cttcattcca ggctgcccac
     3841 cctcaggtga tgcgatctgg aaatttctga cggatttgat cgcaggcaag acacctgaac
     3901 tcggacatgg gctgattaaa tatgactaag cttactacca agaatactgg cgccgataat
     3961 ctggaaaccg cagcgcaccc cgagaatctg cgccgcgtgg caatcgatcc ggtgtcgcgc
     4021 gtggaagggc acggcaaggt gacgctgttg ctggacgaaa atgatcgcgt gcatcaagtg
     4081 cggctgcaca tcgtcgaatt ccgcggcttt gaaaaattca ttcagggccg accatactgg
     4141 gaagcccccg tgatggttca gaggctgtgc ggcatttgcc cggtgagcca ccatctggca
     4201 gcttccaagg cgctggacat gatcgtcggc gccacgctca cgccgaccgc agagaaggtg
     4261 cggcggctga tgcactacgg gcagatattg cagtcacacg cgctgcattt ctttcatctg
     4321 tgttcgcccg acctgttatt cggtttcggc tccgatgttg ccaagcggaa tatcgtcggc
     4381 atcgtggccg cctatccgga aatcgccaaa cagggcgtgt tcttgcgcaa gtatgggcag
     4441 gaggtgatac gcgtcactgc aggcaagcgt gtgcatggta ccggctcgat tcctggcggg
     4501 gtaaacaaaa atgtcagtat cgaggagcgc gattacctgc tcaaggatat agaccagatg
     4561 gtcgagtgga gccgcgatgc ggtcggcctg atccgcaagc tgttcgagca gaatcttgat
     4621 gcctacaatt cttttggccg ctttaaggcg cacaccctca atctggtgcg ccccgacggg
     4681 gcgatggatc tgtatcacgg cggcttgcgt gcacgcgata tggacggcaa tacgctgttc
     4741 gatcactatg attacagtca ttactgggat gtgatcttcg aggatgtgaa gccctggtcg
     4801 tacatgaagt tcccgttcct acgcagtctg gggccggagg agggctggta ccgggtcggc
     4861 cctttgagtc gcgtaacgca gtgcgacttc atcccgtcgc cgctggccga gcaggcgcgc
     4921 cgcgaattca tggctttcga cggcggttcg gtgaccggtg ccacgctggg cttccactgg
     4981 gcgcgcatga tagaaatgtt gcatgccgcc gagatgatca aggagctgct gttcgacacc
     5041 gatctgcagg gcgacgaact ggtcaatatc ggcgagcgtc aggcgcgcgg tgtcggtgta
     5101 atcgaagccc cgcgtggtac gctgatccat cactacaagg tggacgagaa cgaccagatt
     5161 atccgctgca acttgatcgt ttcgacgacc cacaacaatc aggctatgaa cgaggcgatc
     5221 cgtcaggtgg cccgccaata catcgacggc cgtaaactca ccgagggcct gctcaatcac
     5281 atcgaggtcg ccatccgcgc cttcgaccct tgcctttcct gcgcgactca tgcgctgggc
     5341 aagatgccgc tggaggtcga attgctggac gtctccggcg accgggtcga tatgctgacg
     5401 cgttcgtccg atggcacatt ctgcgaagcc tgctgatgac gatgcctgtt ctggtattcg
     5461 ccatcggcaa cgagtcgcgc ggcgatgatg cgttggcccc cttgttgctg cgcgatctgg
     5521 atgagtgggc gaaagcgtgt cggctggacg aacaggtcga attgatcgaa gactatcaac
     5581 ttcaggtcga gcatgtgacc gatctggtcg ggcgtagtgc ggtgctgttc gtcgacgctg
     5641 acatgtcctg cgccgaaccg ttccactttt ccgagatcgc cgccgggcat gacagcagct
     5701 ataccagcca tgcgatgacg cctttcgcgt tgctgcatgc ctaccggcat gtgtatggta
     5761 aagatgcgcc gccgagcttt ctgttgcgag tgcgtgggta cggctttgag ttgggcgatg
     5821 ccctgagcgg tgaggcggcg aaaaatctgg aagccgctac cggactggcc ggagagttgt
     5881 gttgcgagca tgatctgcag cgctggcgtc aacatgttgc gatgcacgat atcgcttgat
     5941 aatcggagga tgaatatgtt cgagtcgcga atacatggac ggggtggaca gggggtggtg
     6001 acggcggcgg agatgctatc catcgccgcc tttctggagg ggcggcatgc ccaggctttc
     6061 cccagtttcg gttcggaacg catgggcgca ccagtggtgg cgttctgccg tattgccgac
     6121 aaagaaatcc ggctgcgtga gcctatcatg gagcctgatg ccatcctcat tcaggatccc
     6181 acgttgttgc atcaggtcga tgtgttttcc ggattgaagc cggacggtta tatcctgctc
     6241 aataccaatc acagctttga agcgttgggg ctggaagaat tcatccgtgg cagacctgcc
     6301 gagcgcctgt gcaccattcc tgcgaccgaa attgccatca aacatatcgg tcgcccgatc
     6361 cccaatgttc ccctgattgc cggattcgca gcactttccg gtctgatccg tctggagtcg
     6421 gtggtcaaag ccatcggcga aaaattttcc ggcaaggtcg cggcaggcaa tatcgccgca
     6481 gcgacagagg cttacaattt ggtgaaggcg atgagggagg agagcactca tgcttaaaca
     6541 gaccgaagga tcgaaagcgg tcgccgaggc gatcgcactg tgccgccccg aagtgatctg
     6601 cgcctatccc atctcgccgc agacgcatat cgtcgaggcg ctgggggaga gcgtcaagag
     6661 cggagaactg acgagctgcg agttcatcaa cgtcgagagc gaattcgccg cgatgtcggt
     6721 cgccatcggc gcatcggctg ccggtgcgcg cgcctatacc gctactgcca gtcaggggct
     6781 gctgttcatg gtcgaggcgg tctataacgc ctccggcctc ggactcccca tcgtgatgac
     6841 ggtcgccaat cgtgccatcg gcgcgccgat caacatctgg aacgaccatt ccgactcctt
     6901 gtcgcagcgc gattcaggct ggatgcagtt gttcgccgag acgaatcagg aggcgctgga
     6961 tctgcatatt caggctttca gactggccga ggaactctca ctgccggtga tggtatgtat
     7021 ggatggtttc attctgaccc atgcctacga gcgcgtggat atgccgaccc agcagcaggt
     7081 cgatgccttt ctgccgccct atgagcctcg tcaggtgctg gatccgcgcg agccggtttc
     7141 gatcggtgcg atggtcggcc ccgaggcttt catggaagtg cgctatctgg cgcacgccaa
     7201 acagatgcag gcgctggagc gcattccgca gctggtggcc gagttcaaac agatattcgg
     7261 acgagacagc ggcggcctga cacataccta ccgcgccgag gatgccgaaa ccatggtggt
     7321 ggcgatgggt tctgtgctgg ggacgatcaa ggacacgatc gacgagatgc gtaacgacgg
     7381 gcacaagatc ggcgtgctgg gcatcaccag ttaccgtccg ttcccactgg cgcaggtgcg
     7441 agacgcatta caaaatgcca agcgcgtcgt ggtgctggag aagagcctgg cagtcggcat
     7501 aggcggaatt ctttccaccg atgtgcgcat ggcgttgtcc ggcatacagt tgcatggcta
     7561 taccgtagtg gccggactgg gcggtcgtgc gatcacgcgc aaatcgctgc acaaactgtt
     7621 ccgcgaggcg gaacaggacg cgctgcaacc gctgaccttt ctggatctgg actggaacgt
     7681 ggtcaacaag caattggagc gcgaacgtgc ggtgcatcgt tccgggcctg cggcagaaag
     7741 cattctgcgc gacatcggca ccgttgcctc aaagatcatt taaggggggt atatgtcatt
     7801 tcaacccgtc aagttctacc aaaccggcac attcaccgtc ggcaaccggc tgttgcatgc
     7861 cgatgagcgc agtgtccagt cgcatcagga tcgcagcaat tcgatcaact ccggacatcg
     7921 cgcctgtcag ggctgcggcg aagcgctggg agcgcgctat gcgctcgatg cagcgatgga
     7981 ggcgtgcgat tcgcaactga tcgcggccaa tgccaccggc tgtctggagg tgttttctac
     8041 tccctatccg gagacttcct ggcagatacc ctggatgcat tccctgttcg gtaacgctgc
     8101 tgcggtggcg accggtatcg cggcagccat gaaggttaaa gggcgcaacg aggtgagggt
     8161 ggtcgcgcag ggcggcgacg gcggcaccac cgacatcggg ttcggatgtt tgtccggcat
     8221 gttcgagcgc aacgacgatg tgttgtatat ctgttacgac aacgaggcct acatgaatac
     8281 cggcgtgcag cgctcgtcgg ccacgccggc tgcggcccgc accgcgacga cgatgccggt
     8341 tggccccaat cccggcaatg tattcggtca gggcaagttc gttcccgcga tcgcgatggc
     8401 gcacgagatt ccctatgtcg ccacggccac ggtggccgac ctgcgcgatc tggaagccaa
     8461 ggtgaagcgt gcgatggagt ttcgcggcgc gcgctatatc cacattctcg taccctgtcc
     8521 gctgggctgg ggtacggctt cgcacgacac catacgcatg gcgcggttgg cgcatgaaac
     8581 cggcttgttt ccggtgttcg aagcggaaca cggcgtgcag gtcagcgcct tgccgatccg
     8641 taaacaggtg ccggtcgaag attatctgaa gccgcagaaa cgcttcgctc acttgttcgg
     8701 tgccaatccc aaactggagg tgatcgccag aattcaggcg cgcgccgacc gcaatatccg
     8761 caagtacggc ctggtgccgc aacaggaggt gctgtgatgg ataagccatt tgcaattacg
     8821 ctcgatcccg gttcatcgct ggccaaccac accggttcct ggcgaaccaa gcgtccggtc
     8881 tatctggatc gcctgccgcc ctgcaatgcg acttgtccgg caggagaaaa tattcagggt
     8941 tggctgtttc atgcagaggc cggaaattac gagcaggcct ggcgcgtgct ggtggagaac
     9001 aaccccttgc ccgccatcat ggggcgggtc tgttaccacc cctgcgaagg cgcatgcaat
     9061 cgcggtcagc tggatagctc cgtgggcatc aactcggtgg aacgttttct cggcgacgag
     9121 gcgatcaagc gcggctggca gtttgacgcg cccgccgccg cttccggcaa aaaagtgctg
     9181 gtggtcggcg ccggcccttc gggcctgtcc gccgcctacc atctggcgcg catggggcat
     9241 ctggtcgaaa ttcatgaggc cgggccgttg tcgggcggca tgatgcgctt cggcatcccc
     9301 aagtaccgtc tgccgcgcga ggtgctggac gccgaggtcg cacgcattct caagctgggt
     9361 gtcacattga aacttgacac caaagtggaa aacatcgaac agagcatgaa gcggggcggt
     9421 ttcgacgcag ccttcctcgc ggtcggtgcc catatcggca agcgcaccta tattccggca
     9481 ggggatgcgg ccaagatact cgatgcggta tcggtgttgc acaatatgga gggcgaagac
     9541 aggccgctgc tgggacgtcg tgtggtcgtt tatggcggag gcaataccgc gatcgatgtg
     9601 gcgcgcaccg ccaagcgtat gggtgctgcc gaggccatca tcgtctatcg ccgcacccgc
     9661 gagaagatgc ccgcgcacga cttcgaggtc gaagaagccc tgcaggaagg cgtgatgatc
     9721 aagtggctgt ccaccatcaa acaggccgat gcgcacagtc tggtcgtgga acgaatggag
     9781 ctggacgaca agggcttccc gcagccgacc ggcgaattcg agaccatgga ggccgattcg
     9841 ctggtgctgg cattggggca agatgtggat ctgtcgttgc tggacggcgt atccggcctg
     9901 gagatcaagg atggcacggt gcaggtcggg atcaacatga tgaccggcca cgacggcatt
     9961 ttcgcgggcg gcgacatggt gccgtcagag cgtaccgtca ccgtggcagt cggccatggc
    10021 aagaaagccg cacaccatat cgacgcctgg ctgaatggtc gcagtcttga ggccgagacc
    10081 aagcacgaga tcgccacctt cgacaaactt aacacctggt attactccga tgcgcctaag
    10141 acggtgcgtc ccgtgctgga catcatccga cgtcagaccg gcttcgacga ggtacagggc
    10201 gggctggacg agaagaatgc actgttcgaa gcgcgccgtt gtttgtcctg cggcaactgc
    10261 ttcgagtgtg acaactgcta cggcgtctgc cctgacaacg cggtgataaa gctcggtccg
    10321 ggcaatgggt acgcaatcaa tctggactat tgcaagggtt gcggaatctg tgtcgcagaa
    10381 tgcccttgc
//
LOCUS       DASUIV010000333         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_923651, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000333 DASUIV010000000
VERSION     DASUIV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923651"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /gene="htpG"
                     /locus_tag="VFW59_11810"
     CDS             complement(<1..1215)
                     /gene="htpG"
                     /locus_tag="VFW59_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX5364939.1"
                     /translation="MTDSATANRETMGFQTEVKQLLQLMIHSLYSNREIFLRELISNA
                     SDACDKLRFEGLHNGALFENDSDLSIHIGYDKVARTLTISDNGIGMSRDEVINNLGTI
                     AKSGTREFFSRLSGDQQKDASLIGQFGVGFYSAFIVADKVTVVTRRAGEHADQGVSWE
                     SDGGGEFSIEMVDRAARGTEITLHLREDQDDLLDGHRLRGIVQKYSNHIVQPILMNKE
                     EWKDGKTEITAEAETVNQASALWARSKSDISDEQYKEFYKHVGHDFDDPLAWTHARVE
                     GRQEYTQLLYIPARAPFDMWDRNARHGIKLYVRRVFIMDDAEQLMPQYLRFVRGVVDS
                     ADLPLNVSREILQESKDIEAIRKGCTNKVLGLLEDMAANDKDKYAQFWAAFGRVLKEG
                     VGEDYANKDKIAG"
     gene            1575..>2169
                     /locus_tag="VFW59_11815"
     CDS             1575..>2169
                     /locus_tag="VFW59_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5364940.1"
                     /translation="MTFEELNLHPSILKAIAETGYTVPTPIQAEAIPEVMAGRDLMAS
                     AQTGTGKTAAFILPALNRLATPAVGNSKGPRVLVLTPTRELAQQVCDAATKYGKHMRF
                     KIISILGGMPYPVQNRLLSSHVDILVATPGRLIDHLERGRIDFSRLEMLILDEADRML
                     DMGFVDDVERIAAATPATRQTLLFSATLEGVIGNLASR"
BASE COUNT          420 a          676 c          583 g          490 t
ORIGIN      
        1 cccggcgatc ttgtccttgt tggcgtagtc ttcgcctacg ccctctttca gcacccggcc
       61 gaacgccgcc cagaactgcg cgtacttgtc cttgtcattg gcggccatgt cttccagcaa
      121 gcccagtacc ttgttggtgc agcccttgcg gattgcctcg atgtccttgg actcctgcag
      181 gatctcgcgc gacacgttga gcggcagatc ggcggaatcg accaccccgc gcacgaagcg
      241 caggtattga ggcatcagct gctcggcatc gtccatgatg aacacgcggc ggacatagag
      301 cttgatgccg tggcgcgcat tgcggtccca catgtcgaac ggcgcacgcg ccgggatgta
      361 cagcaactgg gtatattcct gacggccctc gacacgcgca tgcgtccagg ccagcggatc
      421 gtcgaagtcg tgtccgacgt gcttgtagaa ttccttgtac tgctcgtcgc tgatgtcgct
      481 cttggaacgc gcccacagcg cggaggcctg attcaccgtc tccgcttcgg cggtaatctc
      541 ggtcttgccg tccttccatt cttccttgtt catcaggatg ggctggacga tatgattgga
      601 gtacttctgc acgatgccgc gcagacgatg cccgtcgagc aggtcgtcct gatcctcgcg
      661 cagatgcagc gtgatctcgg tgccgcgcgc agcgcgatcc accatctcga tggaaaattc
      721 gccgccgccg tcggattccc agctcacgcc ctgatcggca tgctcgccgg cgcgacgggt
      781 aaccaccgtc accttgtccg ccacgataaa ggcggaatag aagcccacgc cgaactggcc
      841 gatcaggctg gcatccttct gctggtcgcc ggacaggcgc gagaaaaatt cgcgcgtacc
      901 ggacttggcg atggtgccga gattattgat gacttcgtcg cggctcatgc cgatgccgtt
      961 gtcgctgatg gtcagcgtgc gggcaacctt gtcgtaacct atatgtatgc tcaggtccga
     1021 gtcgttctcg aacagggcac cgttatgcag cccctcgaag cgcagcttgt cgcaggcgtc
     1081 cgatgcattg gaaatcagtt cgcgcagaaa aatctcgcga ttggaataga gcgagtgaat
     1141 catcaactgc aatagctgct tcacttcggt ctgaaaaccc atcgtctcgc gattggcggt
     1201 tgcactgtca gtcatattct ctccattggt taaaataaac ggcgtacagg catctgggga
     1261 tagcgccgga ttttttcaag ggccaacgca ccgccatttc agcccggttc gcagcggcat
     1321 tcaccggcaa gaatatttcg ctggctttta ttttttccgt ccgtataatg cgcacccttg
     1381 ccgcccctgt tgcggcattg gttcatcgtt tccgacctat ctctcgtgcg aattcattcg
     1441 cacctgtcta aagaactcca gcttaattct gggttttccg gttagcaacg atgttttatg
     1501 cgccggttaa tctgtaaccc ctcatcgagg ggtcgtttcc cgcctgcgct tacccttatc
     1561 aaaaggaaag caacatgacg tttgaagaac tgaatctaca cccatccatc cttaaagcta
     1621 tcgccgaaac cggctatacc gtaccgactc ccattcaggc cgaagccatt cctgaagtca
     1681 tggctggtcg cgacctgatg gcctccgctc agactggaac cggcaagact gccgccttca
     1741 ttctgcccgc cctgaaccgt ttggcaacac ctgccgtcgg caacagcaaa ggtccgcgcg
     1801 tactggtgct gacaccgaca cgcgaactgg cgcaacaggt ttgcgatgcc gccaccaaat
     1861 acggcaagca catgcgcttc aagatcatca gcatcctggg tggcatgcct tacccggtac
     1921 agaaccgcct gctgtccagc cacgttgata ttctggtcgc tactccaggc cgcctgatcg
     1981 accatctgga acgcggtcgt atcgacttct cgcgtctgga aatgctgatc ctcgacgaag
     2041 cggatcgcat gctggacatg ggtttcgtcg atgatgtcga gcgcatcgcc gcagcaacac
     2101 cagccacgcg ccagaccctg ctgttctcgg ccacgctgga aggcgtgatc ggcaacctgg
     2161 cttcgcgcc
//
LOCUS       DASUIV010000334         8077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_831084, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000334 DASUIV010000000
VERSION     DASUIV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831084"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2714
                     /locus_tag="VFW59_11820"
     CDS             <1..2714
                     /locus_tag="VFW59_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX5364941.1"
                     /translation="ILQRNATIPVIEAQDQMGVQPDHVYVIPPNRDLTIVHGRLQVTV
                     PTQPHGQRMPIDGFLRSLSEDQADRAIGIILSGTGTDGTLGLRAILGAGGISLVQDPD
                     TAKYEGMPSSAIKAGYATHVLPVEKMPEVLLSGSRIQTIQLERQLPPTATSGINRILM
                     LLRANTGHDFSQYKKSTIGRRIERRMSMHEIDDTEVYARYLKEHPAEIHVLFKELLIN
                     VTSFFRDPEAFDMLKQQIVPLLMENKPEDYVLRVWVAGGATGEEAYSIAMLLREFMDD
                     NQHEFKTQIYSTDLDEDAIAIARGGIYPPNISQDVPQERLRRFFIKEESGYRIKKEIR
                     EMVVFATQSVIKDPPFTKLDLLCCRNLMIYLEPELQNRLIPTFHYALKPHGVLFLSPS
                     ESIGNHTELFTPLDRKWKFYRAIGTVTTTRTVMSTPTSWITQTHTKAPEEMTTQIKAT
                     NYAELTKRALLQSYAPASVVTDKDGNILYVHGDTGKYLRPAPGQATLNIVDMAREGLQ
                     LELRAALNNAGKNYPTLNREVSIRNNEDMQSVSFSLRQLPDSGSNEKLLLISFQDVLR
                     PASEKEGKDAVSANSESRRIVELERELALAREHLQATIEGQQASSEELQSTNEELQST
                     NEELQSTNEELETSKEELQSVNEELITVNAELQFKIEQLVAMQNDMKNLLDNINIGAM
                     FLDAHMVIRRFTREATRVFRLVASDVGRPLADIKSNLENDDMLADAQAVLDTLIPRER
                     EVQAIDGTSFLARIQPYRTLDNVIEGVVLSFAETTELVRVNADRQDALKLTEGIVDTV
                     REPLVVLDSDLRVSYASHSFYQIFQTTPDQTLHRPIYELGNRQWNIPKLRDLLETILP
                     RDQSFEDYVVEGDFPAIGHSRFTLNARRIIGQAKNSPLILLAMEIDA"
     gene            2711..3937
                     /locus_tag="VFW59_11825"
     CDS             2711..3937
                     /locus_tag="VFW59_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5364942.1"
                     /translation="MNDKRPAAARMASLREDAEALIPHALTPETQDEVLHELRVHQIE
                     LEMQAEELRSAEVALEVSRDRYLDLYDFAPVGYLSLNRDGLVTEINLTGAALLGIERA
                     KIRQRRFINFIAPSDIERWHRHFTGLFNSREKRRCEMALIHADGSSFHARLDALHMRT
                     DSGAETLRIAFSDISESKLAMLELEESRKLLRELAARNETLLEDERKRISREVHDELG
                     QLLTVLRMDISLQRIQFSHYDPALFKMTQGMLKLTDKIINEVRNVATNLRPTALDCGI
                     LPALAWLCDEFNHHSTQACTLLAPDGPIELNEASTLALFRIVQESLTNVLRYAEAHSV
                     EITIRREVDCIVTTVSDDGKGFDPSATAPKKSFGLLGMRERALAAGGEIEISSAPSLG
                     TTITVRIPIHPPKDIS"
     gene            3934..4569
                     /locus_tag="VFW59_11830"
     CDS             3934..4569
                     /locus_tag="VFW59_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5364943.1"
                     /translation="MIRLIIADDHAVVREGLERIFMLAADIELAAQVANGDELFQQLR
                     AGANFDLLLLDMTMPGVNGISLIGNIKALRPNLPILVFSMHNETSIALRAIKAGASGY
                     LTKDCDPEILLNAIRKVINNGTYLTPELAKHLAFDSLIPDQAAPHTLLSDREFEVFHM
                     LIASNRLTEIAEHLSISEKTVSSHKHNLMKKLKLHSMSELLHYAYEHKLVV"
     gene            4808..5005
                     /locus_tag="VFW59_11835"
     CDS             4808..5005
                     /locus_tag="VFW59_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364944.1"
                     /translation="MSHTLSQPEEIDLVLIEMERMDCNSSPFSSDKKTTLQTSPTMPN
                     YGYVSPYRAAHIRSMQSRTNP"
     gene            5030..5236
                     /locus_tag="VFW59_11840"
     CDS             5030..5236
                     /locus_tag="VFW59_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HEX5364945.1"
                     /translation="MKLEEIRSIAKSRGISPGKASKGELIKRIQSDEGNFDCFATAYH
                     GECNQTGCAWRADCFAASQYGELS"
     gene            5233..5682
                     /locus_tag="VFW59_11845"
     CDS             5233..5682
                     /locus_tag="VFW59_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5364946.1"
                     /translation="MNLVKWDPFKELEDVSNRLNQIFGRSLARTEPTQNMLAMADWEP
                     SVDISETDTAYLIKGEIPGVKKEDVKITIQNGMLTIQGERKQEKEEKGKKFHRVECSY
                     GSFMRSFRLPGDTDESKVEAEFKDGMVSVTLPKSEKAKPKAIEVKVS"
     gene            5723..5926
                     /locus_tag="VFW59_11850"
     CDS             5723..5926
                     /locus_tag="VFW59_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5364947.1"
                     /translation="MATGIVKWFNDAKGFGFITPDDGSDDLFAHFSAINMNGFKSLKE
                     GQKVSFEITQGQKGKQAANIQAG"
     gene            6092..6439
                     /locus_tag="VFW59_11855"
     CDS             6092..6439
                     /locus_tag="VFW59_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5364948.1"
                     /translation="MLDSKRLTLALWMFLSLPGYTLAGTVSAESRGELLYTTHCIACH
                     AADIHWREQKLATDWISLVAQVNRWQASTGLIWSNDEIEDVARYLNKLHYGFPAPPHK
                     DYSQNKKSDRMRH"
     gene            6509..6889
                     /gene="raiA"
                     /locus_tag="VFW59_11860"
     CDS             6509..6889
                     /gene="raiA"
                     /locus_tag="VFW59_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5364949.1"
                     /translation="MQTPLQITVRDFEHSDALETRIREKAKKLEEFFNQITSCRVVVE
                     MPHKHHHQGKHFNVRIDIGVPGKEIVVNRDHDEDIYVALRDAFGAAERQLKDYAHKLR
                     GEIKTHDQRRTNGNDESGGAEAAD"
     gene            6986..>8077
                     /gene="amrS"
                     /locus_tag="VFW59_11865"
     CDS             6986..>8077
                     /gene="amrS"
                     /locus_tag="VFW59_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX5364950.1"
                     /translation="MDEPISVAEQHPAKYWHLLSDGRIQCDLCPRDCKLHDGQRGACY
                     VRGRVADHLVLTTYGRSSGFCIDPIEKKPFNNYYPGSSVFSFGTAGCNLACKFCQNWD
                     ISKSRSFDKLADQASPEAIALAAERMGCKSVAFTYNDPVPFAEYAMDTADACHARGIK
                     TVAVTAGYIHDAPRREFFAKMDAANVDLKGFTDEFYVKLCGAHLQPILDTLIYLRQET
                     DVWLELTTLLIPGHNDSDEELGRMSAWVAKELGPDVPLHFSAFHPDYKMMDTPPTPPE
                     TLGRARKIAMAAGLHYVYTGNVHNREGDTTFCPSCKHAVIVRDWYDIEQYRLTPEGRC
                     PDCGAQIAGHYQKFTGAFGQRRIPIDMRDM"
BASE COUNT         1973 a         2436 c         2137 g         1531 t
ORIGIN      
        1 agatcctgca acgcaatgcc accatcccgg tgatcgaagc ccaggaccag atgggcgtgc
       61 aacccgatca tgtttacgtg atccctccca accgagacct caccatcgtt cacggcaggc
      121 tgcaggtgac cgtccccact cagccgcacg gacagcgcat gcccatcgat ggctttctgc
      181 gctccctgtc cgaggatcag gcagaccgcg cgatcggcat catcctgtct ggcaccggta
      241 ccgatggcac gctgggacta cgcgcgatcc tcggggccgg gggcatctcg ctggtacagg
      301 atccggacac ggccaaatac gagggcatgc cgagcagcgc catcaaggcc ggttatgcca
      361 cccatgtcct gccggtggag aagatgccgg aagtgctgct gtccggctcg cgaatccaga
      421 ccatacaact ggaaagacag cttcccccga ctgcgaccag cggcatcaat cgcatcctga
      481 tgctgctgcg cgccaacacc gggcacgatt tttcgcaata caagaagagc accatcggtc
      541 ggcgcatcga acggcgcatg tccatgcacg agatcgatga caccgaggtc tatgcacgct
      601 acctgaaaga acatccggcc gagatacatg tgctgttcaa ggagctgctg atcaacgtca
      661 ccagcttctt tcgcgatccc gaggcattcg acatgctgaa gcagcagatc gtgccactgt
      721 tgatggagaa caaacccgag gattatgttt tgcgggtctg ggtcgcaggc ggcgcgaccg
      781 gcgaagaggc ctattcgatc gcgatgctgc tgcgcgagtt catggatgat aaccagcatg
      841 aattcaagac ccagatatac agcaccgatc tcgatgagga tgccatcgcc atcgctcgcg
      901 gcggcatcta tccccccaac atctcgcagg atgtccctca ggaacggtta cgccgctttt
      961 tcatcaagga agagtccggc taccgcatca agaaggagat ccgcgagatg gtggtgttcg
     1021 ccacccagag cgtgatcaag gatcctccgt tcacgaaact ggacctgctc tgctgccgca
     1081 acctgatgat ctatctggag ccggaactgc aaaatcgtct catccccacc ttccattacg
     1141 cgctcaagcc tcacggcgtg ctgtttctgt cgccgtctga gagcatcgga aaccataccg
     1201 aactgttcac cccgctcgac agaaaatgga agttctaccg agccatcggc acggtgacga
     1261 cgactcgcac ggtcatgtcc accccgacct catggatcac ccagacacac acaaaagcac
     1321 ccgaagaaat gacaacgcag atcaaggcaa ccaattacgc cgaattgacc aaacgagccc
     1381 tgttgcagtc ctatgcaccg gcatccgtgg tgaccgacaa agacggcaac atcctctatg
     1441 tgcatggcga cacgggcaaa tatctgcgtc cggcacccgg tcaggcaacg ctcaacatcg
     1501 tcgacatggc acgcgaaggc ctgcaactgg aactgcgtgc ggccttgaac aacgcaggca
     1561 aaaattaccc caccctgaac cgcgaagtct cgatcaggaa caatgaggat atgcagagcg
     1621 tcagcttcag cctgcgccaa cttccggatt cgggcagcaa cgaaaagtta ttgctgatca
     1681 gttttcagga tgtgctgcgg ccagcatcgg aaaaagaagg caaagatgca gtctcggcga
     1741 acagcgaatc gcgacgaata gtcgaactgg aacgcgaact ggcgctcgcc cgggaacacc
     1801 tgcaggcgac catcgaggga caacaggcct ccagcgaaga gctgcagtca acgaatgagg
     1861 aactgcaatc caccaacgag gagctgcagt cgaccaacga ggagctggag acctccaagg
     1921 aagagctgca gtccgtcaat gaagagctca tcacggtcaa tgccgaactc cagttcaaga
     1981 tcgagcaact ggtcgccatg cagaacgaca tgaagaatct gctcgacaac atcaacatcg
     2041 gcgccatgtt cctcgacgca cacatggtca tccgtcgttt cacgcgcgaa gcaacgcggg
     2101 tgttccgact ggtcgcatcg gacgtgggac gaccgctcgc cgacatcaag tccaatctcg
     2161 agaacgatga catgctggcc gatgcgcagg ccgtgctgga caccctgata ccgcgcgagc
     2221 gcgaagtgca ggccatcgac gggaccagtt ttctggcgcg tatccagccc tatcgcacac
     2281 tggacaatgt gatcgaaggc gtcgtgctga gcttcgccga gaccaccgaa ctggtcaggg
     2341 tcaatgccga cagacaggat gcactcaaac tgaccgaagg catcgtcgac accgtgcgcg
     2401 aaccgctcgt ggtactggat tccgacctgc gggtatccta tgccagccac tcgttctacc
     2461 agatttttca gaccacaccg gatcaaacac ttcatcgccc gatttatgag ttgggcaatc
     2521 gccagtggaa catcccgaaa ttgcgcgatt tgctggaaac catcctgcca cgcgaccagt
     2581 cttttgaaga ctatgtggtt gaaggcgact tccctgccat aggccacagt aggtttacgc
     2641 tgaatgcaag acgcatcatc ggacaggcca agaactcacc attgatcctg ctggcaatgg
     2701 agatcgatgc atgaacgaca agcgaccagc tgcggcaagg atggccagct tgcgtgaaga
     2761 tgcggaagcg cttattcccc atgccctgac gcccgagact caggatgaag tactgcacga
     2821 actcagggtg caccagattg aactcgagat gcaggccgaa gaattacgct ctgccgaggt
     2881 tgcactggaa gtatcccgcg accgttatct ggatctctac gactttgccc ctgtcggcta
     2941 tctctcgctc aaccgtgacg ggctggtcac cgagatcaac ctcaccggcg ctgcgctgct
     3001 tggaatagaa cgggcgaaaa tacgacagcg ccgattcatc aacttcatcg cgccatccga
     3061 catcgagcga tggcatcgcc actttaccgg gctattcaat agccgcgaaa agcggcgctg
     3121 cgaaatggcg ctcatccatg cggacgggag ttcattccac gcaagactgg atgccttgca
     3181 catgaggact gacagcggag ccgagacgtt acgcatcgcg ttctccgaca tcagcgagag
     3241 caaactcgcg atgctcgagc tcgaagaatc ccgcaagctg ctgcgcgaat tggctgcgcg
     3301 caacgagaca ctgcttgagg acgagcgcaa acgcatctcg cgcgaggttc atgacgagct
     3361 tggacaactg ctgactgtat tgcgcatgga catctcgctg cagcgcatcc agttttccca
     3421 ctacgatcca gccttgttca aaatgactca gggcatgctg aaactgaccg acaagatcat
     3481 caatgaagtg cggaacgtag cgaccaacct gcggccaacc gcgctggact gcggtatcct
     3541 ccctgccctc gcctggttat gcgacgaatt caaccatcac tccacgcagg cctgtacctt
     3601 gctggccccc gacgggccga tcgagctgaa cgaggcatcc actctggcgc tgttccgtat
     3661 cgtgcaggaa tcgctcacca atgtccttcg ctacgccgaa gcgcacagcg tcgagatcac
     3721 gatcaggcgg gaggtcgatt gcatcgtcac gacggtgagt gacgatggca agggcttcga
     3781 tccgtccgcc actgcgccga aaaaatcctt cggcctgcta ggcatgcgcg aacgcgctct
     3841 ggccgctggc ggcgaaattg agatcagcag cgcgccatcc ctcgggacca ccatcactgt
     3901 ccgcataccg atccaccccc caaaggatat ttcatgatcc gcctgatcat tgccgacgat
     3961 catgccgtgg tgcgtgaagg gctggagagg attttcatgc tggctgctga tatcgagttg
     4021 gctgcgcagg tcgccaatgg ggacgaactg ttccagcagc tgcgcgctgg tgcgaacttc
     4081 gatttgctgc tgctggacat gaccatgccc ggtgtgaacg gaatcagcct gattggcaac
     4141 atcaaggcgc ttcgcccgaa tctgcctatc ctcgttttca gcatgcacaa cgagacctca
     4201 atcgccctgc gggccatcaa ggccggagcc agtggctatc tgaccaaaga ctgcgacccg
     4261 gaaatactgc tcaatgcgat ccgcaaagtc atcaacaacg ggacttacct cacgcccgag
     4321 cttgccaaac acttggcgtt cgactccctc atccccgatc aggccgcacc tcacaccctg
     4381 ctttctgacc gggagttcga ggtgtttcac atgctgatcg ccagcaatcg cctcaccgag
     4441 atagccgagc atctgtccat cagtgaaaag accgtgagct cgcacaaaca caatctgatg
     4501 aaaaaactga agttgcacag catgtcggaa ctgctgcatt acgcctacga gcataagctg
     4561 gtagtctgat ccgagtgccg agtcagccta taggggtttc cttaccctct gcacatcatt
     4621 ttcctatggg cgattcaata cttccctgat tccccccccc tccctgtcgg cagataatca
     4681 actctcccga aatctccgca tcccatcagg ctaaataacg gcttgttaag cgcaaggttt
     4741 ggctgcatcg cttttgccac cttggattct ctggcatacc tttatgacag tattaagagg
     4801 aaacatcatg agccatactc tttcacaacc cgaagaaatc gacctcgtcc tgattgaaat
     4861 ggagcgcatg gactgtaact ccagcccgtt ctcgagcgat aaaaaaacca ctcttcagac
     4921 atcgcccacc atgccgaact acggctacgt ttctccctac cgcgcggcgc atatcagatc
     4981 gatgcagagc cgcacgaacc cgtagttgta atctttatca ggagacatca tgaagcttga
     5041 agaaattcgc agcatcgcca agtcacgcgg catcagtccc ggcaaagcct caaaaggcga
     5101 actgatcaaa cggatacaga gcgacgaagg caatttcgac tgttttgcca cggcttacca
     5161 cggtgagtgc aatcaaaccg gttgcgcctg gcgtgcggat tgtttcgccg catctcaata
     5221 tggagagcta tcatgaatct ggtcaaatgg gatcctttca aggaactgga agatgtttca
     5281 aatcggttga atcaaatctt cggccgctcg ctggcacgca ctgaaccaac tcaaaacatg
     5341 ctggccatgg ccgactggga gccctccgtc gacatcagcg agaccgacac ggcttacctg
     5401 atcaagggcg agattcccgg cgtcaagaaa gaagacgtca agatcaccat tcagaacggc
     5461 atgctgacca ttcagggcga acgcaagcag gagaaggaag agaaaggcaa gaagttccac
     5521 cgtgtcgaat gcagttacgg cagcttcatg cgcagcttcc gcctgcccgg cgatacggac
     5581 gagagcaagg tcgaggccga attcaaggat ggcatggtca gcgtcacgct acccaaatca
     5641 gaaaaggcaa aacccaaggc gatcgaagtc aaggtctcgt aacaaaaccg ctgttaaatt
     5701 taattggcaa ggagaaggtt tcatggcaac aggcatagtc aaatggttca acgatgcaaa
     5761 aggatttgga tttatcaccc cagatgatgg aagtgatgat ttgttcgcgc atttctccgc
     5821 gatcaacatg aatggcttca agtcgctcaa ggaaggccag aaggtcagct tcgagatcac
     5881 gcagggccaa aagggcaagc aggccgccaa cattcaggct ggctgatccg cccgaacatt
     5941 catggtcata aagtcagcgc gtccgggccg cttcgtctgc ctgccacgcg ctgatttgtt
     6001 tcatccggac actcacaccg gaggtctcgt ccgcccctct atcgatctcg ggtgaattga
     6061 agtttcgcta tttgatgact acaggattcc tatgctcgat tcaaaacggt taaccttggc
     6121 gctatggatg ttcttgagct tgcctggcta cacgctggcc ggaaccgtca gtgcagaatc
     6181 acgaggcgaa ctgctctata ccacccactg catcgcatgc catgctgccg acatccattg
     6241 gcgcgaacag aaacttgcaa ctgactggat cagcctcgtg gctcaggtga accgctggca
     6301 agccagcacc ggcctcatct ggagcaatga tgagatcgaa gatgtcgccc gctatctgaa
     6361 taagctccat tacggcttcc cggcgccccc ccacaaggat tattcgcaaa acaaaaaatc
     6421 ggatcgcatg cggcattgat ttctggtggc ctgaaaagga accatcggtg ataatccccc
     6481 tcaggcattc aaccaaaagg ggagcaccgt gcagacacca ttacagatta ctgttcgtga
     6541 ctttgaacat tccgatgcac tggaaactcg tatccgcgaa aaagcgaaga aactggagga
     6601 gttcttcaat caaatcacct cctgccgggt cgtggtcgag atgccccaca agcaccatca
     6661 tcagggcaag catttcaacg tacgcatcga tatcggcgta ccgggcaagg agatcgtggt
     6721 gaaccgcgat catgacgagg acatttatgt cgccctgcgc gatgccttcg gtgcggccga
     6781 gcgccaactc aaggactatg cgcacaagct ccgtggcgaa atcaagacgc acgatcagcg
     6841 acgcacaaat ggcaatgacg aatctggcgg ggcggaagcg gcagactgac agcgcttcgc
     6901 agtacggatg taaggctggt ctgccacagg ccagccggaa tggtatcaag tgacataaac
     6961 cccttggaca atcaggagcg gtgacatgga cgaacccatc agtgttgcag aacagcatcc
     7021 ggccaagtac tggcacctct tgagcgacgg acgcatccag tgcgatctct gtccgcgcga
     7081 ttgcaagctt cacgacggcc agcgcggcgc atgctacgtg cgcggccggg tcgcagacca
     7141 cttggtgctc accacctatg gacgctcgtc cggattctgc atcgacccca tcgagaagaa
     7201 gcccttcaac aactactatc ccggctccag cgtgttctcc ttcggcacgg cgggctgcaa
     7261 tctcgcctgc aagttctgcc agaactggga catctccaag tcgcgcagtt tcgacaaact
     7321 ggccgatcag gccagccctg aagccatcgc actcgccgcc gaacgcatgg gctgcaagag
     7381 cgtggcgttc acctacaacg atcccgtacc cttcgctgaa tacgcgatgg acacggcgga
     7441 cgcctgtcac gcgcgcggca tcaagaccgt cgcggtcacc gcaggctaca tccacgatgc
     7501 gccacgccgc gagttcttcg ccaagatgga cgccgccaat gtcgacctga aaggtttcac
     7561 cgacgagttc tacgtcaaac tctgtggcgc acatctgcaa ccgatactgg acaccctgat
     7621 ctatctgcgt caggagaccg acgtatggct ggagctgacc acgctgctga tccccggaca
     7681 caatgattca gacgaggaac ttggccggat gagcgcttgg gtcgccaagg aactgggacc
     7741 ggatgtaccg ctgcactttt ccgcattcca ccccgactac aagatgatgg acaccccgcc
     7801 cacgcccccg gagacgctgg gcagggcaag gaagatcgcg atggctgcag ggctgcacta
     7861 cgtctacacc ggcaacgtac acaaccgcga gggcgacacc accttctgcc cgtcctgcaa
     7921 gcacgccgtg atcgtacgcg actggtatga catcgaacaa taccgcctca cacctgaggg
     7981 ccgctgcccg gactgcggtg cacagatcgc cgggcactac cagaaattca ccggtgcatt
     8041 cggccagcgc cgtatcccga tcgacatgag ggacatg
//
LOCUS       DASUIV010000335         6853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_275733, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000335 DASUIV010000000
VERSION     DASUIV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275733"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="mltB"
                     /locus_tag="VFW59_11870"
     CDS             complement(<1..786)
                     /gene="mltB"
                     /locus_tag="VFW59_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEX5364951.1"
                     /translation="MRFILSLLLLGSLSAHAVELPGIPQFIDEMVVKHQFRRDELENV
                     FAHAQHRPAIIEAISRPATFKPWPEYRASFVNRQRVKFGLIFWDKYRMTLRRAEQKYG
                     VPQEIIVALIGVETIYGRNAGNYRVLDALTTLAFDYPRRAVFFRDELENYLLLAREQQ
                     FDLLSLRGSYAGAMGVPQFMPSSYRKYAVDFNGNRKIDLLREDSDAIGSVANYLQGYG
                     WLAREPVAVRAHLSATACPGDIAMMRSLALWAEDGVEPDVKKPV"
     gene            871..1833
                     /gene="trxB"
                     /locus_tag="VFW59_11875"
     CDS             871..1833
                     /gene="trxB"
                     /locus_tag="VFW59_11875"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5364952.1"
                     /translation="MTSATQHHRLIILGSGPAGYTAAVYAARANLNPVLITGMAQGGQ
                     LMTTTEVDNWPADPNGVQGPELMERFQKHAERFNTQMIFDHIHTAKLQQKPFLLVGDA
                     GSYTCDALIICTGASAQYLGLPSEEAFMGRGVSGCATCDGFFYRGQQVAVVGGGNTAV
                     EEALYLANIAAHVTLVHRRDSFRAERIMIDHLMEKVKEGKITLQLNSVLDEVLGDASG
                     VTGMRVKNLQSNEKQDIALSGVFIAIGHKPNTDIFAGQLEMDNGYITTRGGSNGNATA
                     TSISGVFAAGDVQDQIYRQACTSAATGCMAALDADKYLAAMGKA"
     gene            1830..2351
                     /locus_tag="VFW59_11880"
     CDS             1830..2351
                     /locus_tag="VFW59_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX5364953.1"
                     /translation="MSKPAEDDEAALFRAVVGEVTPLPQQNRIASPPPPRKPWRHHGH
                     TPEAIPDTLSDFSGSAPPDEYLSNGLSRMTLRKLRRGYWPVQDVLDLHGLNTEAARKL
                     LQAFLHQANQNGLRCVLVIHGKGMNSRNGEAVLKLNTRHWLIQHPQVLAWCDAPPRDG
                     GGGAVLALLKTNY"
     gene            2396..2833
                     /locus_tag="VFW59_11885"
     CDS             2396..2833
                     /locus_tag="VFW59_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5364954.1"
                     /translation="MNKYLLIFLMLCGTNAYAEIHRWVDDHGKVHYTDQPPVGVDATE
                     LRSAPAPGSAPAAAKTFAEREADLKKAQQTKKEADARAAQKQANAEAEKYNCSVAQQA
                     LLSLKSGRRVAEYDANGERSFLGESELQQRTAKAQEEVAKWCK"
     gene            complement(3023..6544)
                     /gene="smc"
                     /locus_tag="VFW59_11890"
     CDS             complement(3023..6544)
                     /gene="smc"
                     /locus_tag="VFW59_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX5364955.1"
                     /translation="MRLSQIKLAGFKSFVDPTHIPLPGQIVGVVGPNGCGKSNVIDAL
                     RWVLGESKASALRGSSMQDVIFNGSSKRKPVSRASVELVFDNSLGKAAGQWSSYAEIS
                     IKRVLQRDGESSYFINNQHVRRKDITDIFLGTGLGARAYAIIEQGMISRIIEAKPEEL
                     RIFLEEAAGVSKYRDRRRETELRIGDTRDNLLRVSDILQELDKQLLHLGEQAEVAKQY
                     RAFEAQRNTTQQLFWLVKRQEAESQRVRYVQLTEKTRNELEAETALLREIESKLEATR
                     SGHYQLADALHVRQGELYTVNAEIARLEQQIKHVAEQRHRVAQQIANTERQIEQQRQQ
                     RQGVHSLLEHWQHQQESAVVKLADAEMNAEAESMNLPQAEDAAREAQERYNALQREQM
                     QLQQKLQLAETQRSHVLQNIQQLESRRQRLLQEKDNLPRVDDAALQEAQEEQAALEMQ
                     RAEQVERLAQLQEQLPLADNERRNQRTSLQQQERMLAQTEARLSALQQLQQRLDNDQK
                     LKAWLQQQQLDRLPRLWQSISIGEGWEDALEAVLRERLNAIAMPQLADAAAWSEAPPA
                     KLALFAPNAEAASADASPPQLTPLLNYVQCQDAQVLPAMRDWLTHVYAVADVEQAYAQ
                     RTQLPKGGWFVTKQGHLVGMNSVLFHAPDSQLHGVLARQREIEKLEQELDEQNRALEY
                     TREQVAQAEQQYQSIEIQIGPLRTAGNELQQRQHALQMQILKLTQAHERSSERLVQID
                     QELQELAEQLIGTQYQQSELDEQMALSREQIAAFYSQVEDAQRQVNALDISLREQRSR
                     AQLAQHALQEARFFAKTCVEKIADLQHNTEQIAESLAQFELNLGQLHEDMTGNEDEVV
                     LEQLQAALDSRQGFEQALAQSRNILENATVELQKLEHDRLSCEHKLNPLREKLNELAL
                     KEQEARLHFEQWAEQLQGVDEQALLPLIEAGNNKPNVLQNELNRLDAGIESLGAVNLA
                     ALEELQTAAERKSYLDAQAADLNEAMATLEDAIRRIDKESRDLLMDTYNKVNEHLAEM
                     FPVLFAGGEARLVLTGEEILDSGVQVMAQPPGKKNSTIHLMSGGEKALTAIALVFSLF
                     QLNPAPFCLLDEVDAPLDDTNTERLCALIKRMSLQTQFIFISHNKITMEMAKQLVGVT
                     MQEKGVSKVVAVDMEEALRMRDEVVVV"
     gene            6607..>6853
                     /gene="queF"
                     /locus_tag="VFW59_11895"
     CDS             6607..>6853
                     /gene="queF"
                     /locus_tag="VFW59_11895"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX5364956.1"
                     /translation="MTTQPNKTLETFPNPNPGRDYHIHMEIPEFTCLCPKTGQPDFAT
                     LFLDYIPDQRCVELKSLKLYVWSFREEGHFHEDVTNRI"
BASE COUNT         1304 a         2248 c         1982 g         1319 t
ORIGIN      
        1 caccggtttt ttgacgtcag gctcaacgcc atcctcggcc cataaagcca acgaacgcat
       61 catcgcgata tcaccgggac aggcggtggc gctcagatgc gcccgcacgg cgaccggctc
      121 tctggcgagc cagccatagc cttgcagata gttggcgaca ctgccgatcg catcgctgtc
      181 ttcgcgcaac agatcgatct tgcgattgcc gttgaaatcc accgcatatt tgcgataact
      241 gctgggcatg aactgcggca cccccatcgc tccggcataa gaaccgcgca aactcaacag
      301 atcgaattgc tgctcgcgtg ccagcaacag ataattttcc agctcatcac gaaagaatac
      361 ggcacggcgc ggataatcga acgccagcgt ggtcaacgca tccagcaccc ggtagttgcc
      421 tgcattcctg ccgtagatcg tctccacccc gatcagcgcg acgatgatct cctgcggcac
      481 accgtacttc tgctcggcac ggcgcaacgt catgcgatat ttgtcccaga agatcagacc
      541 gaatttaacg cgctggcggt tgacaaaaga cgcgcggtat tccggccagg gcttgaaggt
      601 cgccgggcgg gagatcgcct cgatgatcgc aggacggtgc tgcgcatggg caaacacgtt
      661 ttccagctca tcgcgcctga attggtgctt gacgaccatc tcgtcgatga actgggggat
      721 acctggaagt tcgacagcgt gagcggagag agaacctagt aacagcaggg ataaaatgaa
      781 acgcatagtg gacgcatttt aactcgacag gcaggcctgc cgctgctaaa tttcgcccat
      841 tcagagcaat tcgacacgaa agcatacatc atgaccagcg ccactcaaca ccatcgcctc
      901 atcattctcg gctccggccc ggcgggttac accgccgccg tgtacgccgc gcgagccaac
      961 ctcaatccgg tgctgatcac cggcatggca caaggcggac aactgatgac cacgaccgaa
     1021 gtggacaact ggcccgccga cccgaacggc gtgcaaggcc cagaattgat ggaacgcttc
     1081 cagaaacatg ccgagcgttt caacacgcaa atgatcttcg accacatcca cacggccaag
     1141 ctgcagcaga aacccttcct gctggtcggc gacgcgggca gttacacctg cgatgcactg
     1201 atcatctgca ccggcgcctc ggcgcaatac ctcggcctgc cttccgaaga agccttcatg
     1261 ggacgcggcg tatccggctg tgcgacctgc gacggcttct tctatcgcgg ccagcaagtg
     1321 gccgtggtcg gcggcggcaa taccgcagtc gaagaggcgc tgtatctggc caatatcgcc
     1381 gcgcacgtga cgctggtgca tcgccgcgac agcttccgcg ccgaacgcat catgatcgac
     1441 cacctgatgg agaaggtgaa agaaggcaag atcaccctgc aactgaattc cgtactggac
     1501 gaagtgctgg gcgacgccag cggcgtgact ggcatgcgcg tcaaaaacct gcagtcgaac
     1561 gagaaacagg acatcgcgct cagcggcgtg ttcatcgcca tcggccacaa gcccaatacc
     1621 gatatcttcg ccggacaact ggagatggac aacggctaca tcacgacgcg cggcggttcg
     1681 aacggcaacg ccacggcaac cagcatctcc ggcgtattcg cggccggcga cgtgcaggac
     1741 cagatctacc gtcaggcctg caccagcgcc gcgaccggct gcatggccgc actggatgcc
     1801 gacaagtacc tcgctgccat gggcaaggct tgagtaaacc ggcagaggac gacgaggccg
     1861 cgctgttccg cgcggtcgtc ggcgaggtga cgcccctgcc gcaacagaac cgcatcgcct
     1921 cgccgccccc gccacgcaag ccttggcggc accacggcca tacccccgag gcgatacccg
     1981 acacgctgtc ggattttagc ggcagcgccc cccccgatga atacctgagc aacggcctgt
     2041 cgcgcatgac cttgcgcaaa ctgcggcgcg gatactggcc ggtacaggac gtactcgatc
     2101 tgcatgggct caacaccgag gcggcacgca aactcttgca ggcctttctg catcaggcga
     2161 atcagaacgg attgcgctgc gtgctggtga ttcacggcaa aggcatgaac agccgcaacg
     2221 gcgaagccgt gctaaagctg aacacccgcc actggctgat acaacacccg caggtgctgg
     2281 cctggtgcga cgccccgcca cgggacggtg gcggcggtgc ggtgctggcg ctgcttaaaa
     2341 cgaattactg aacatgctag actgcgcaga atcatcgaca cggagacctg tagccatgaa
     2401 taaatacctg ctcattttcc tgatgctatg tggcacgaac gcctatgctg aaatccatag
     2461 atgggtcgat gatcacggca aggttcatta caccgaccag ccgccggtcg gtgtcgatgc
     2521 gactgaactg cgctcggcac ctgccccggg cagcgccccc gcagcagcca aaacctttgc
     2581 cgaacgcgaa gcggacctga agaaggcaca gcagaccaag aaggaagccg acgctcgcgc
     2641 cgcccagaaa caggcgaatg ccgaggccga aaaatataac tgcagcgtcg cacaacaggc
     2701 cctgctctcc ctgaagtcgg gaaggcgcgt agccgaatac gatgcgaatg gcgagcgaag
     2761 ctttctggga gagagcgaac tgcagcaacg caccgcaaag gctcaggaag aagtcgccaa
     2821 gtggtgcaaa taacgggctt ctgtacgaca gagcagtaag ccattgacct gccccctggc
     2881 tttagcgtgc agcgccatga agtaacaact agccattcaa ccaagccatc aaacaacgat
     2941 ggcaagttgc tggttatgtc ccttggttac ctcgcagagg ttcttgcacc ctcgggtgtt
     3001 gcgccctgcc gggtcgtttc gactagacca ccacgacctc gtcgcgcatg cgcagggcct
     3061 cttccatgtc caccgccacc accttggaca cgcccttttc ctgcatggtc acgccgacca
     3121 gttgcttggc catctccatg gtgatcttgt tgtggctgat gaagatgaac tgggtctgca
     3181 aagacatgcg cttgatcagc gcgcacaacc gctcggtgtt ggtgtcgtcc agcggcgcat
     3241 ccacctcgtc gagcaggcag aacggcgcgg ggttgagctg gaacagcgag aacaccagcg
     3301 cgatggcggt cagcgccttt tcgccgccgg acatcaggtg gatggtgctg ttcttcttgc
     3361 ccggaggctg cgccatcacc tgcacgccgc tgtcgaggat ctcctcgccg gtcagcacca
     3421 gccgcgcctc gccgccggcg aacagcaccg ggaacatctc ggccagatgc tcgttgacct
     3481 tgttgtaggt atccatcagc aggtcgcgcg attccttgtc gatacggcgg atcgcgtctt
     3541 ccagcgtcgc catcgcctcg ttcagatcgg ccgcctgcgc atccagatag gacttgcgct
     3601 ctgctgcagt ctgcaactct tcgagcgccg cgagattcac cgcccctaac gactcgatgc
     3661 ccgcatccag acgattcagc tcgttctgca ggacgttcgg cttgttgttg ccggcttcga
     3721 tcaacggcag caaggcctgc tcgtccacgc cctgcaactg ttccgcccat tgctcgaaat
     3781 gcagccgggc ttcctgctcc ttcagcgcca gctcattgag cttctcgcgc agcggattga
     3841 gtttgtgctc gcaggacagg cggtcatgct ccagcttctg cagctcgacc gtggcgttct
     3901 ccagaatgtt gcgcgactgg gccaaggcct gctcgaagcc ctgacggcta tccagcgcgg
     3961 cctgcaactg ctccagcacg acctcgtcct cgttgccggt catatcctcg tgcaactggc
     4021 ccagattcag ctcgaactgc gccagcgact cggcgatctg ctcggtgttg tgttgcaggt
     4081 cggcgatctt ctcgacgcag gtcttggcga agaaacgcgc ctcctgcagc gcatgttgcg
     4141 ccaactgtgc gcggctgcgc tgctcccgca ggctgatatc gagcgcattg acctgacgct
     4201 gcgcgtcttc cacctgagaa tagaaggccg cgatctgctc gcgcgacagc gccatctgct
     4261 cgtccagttc gctctgctgg tactgcgtgc cgatcagctg ttcggccagc tcctgcagct
     4321 cctgatcgat ctggacaagg cgttcgctgc tgcgctcgtg cgcctgggtc agcttgagta
     4381 tctgcatctg cagcgcatgc tggcgctgct gcagttcgtt gcccgccgta cgcagcggcc
     4441 cgatctggat ctcgatggac tgatactgct gctcggcctg cgcgacctgc tcgcgggtgt
     4501 actccagcgc gcggttctgc tcgtccagtt cctgttccag cttttcgatc tcgcgctgcc
     4561 ttgccagcac gccgtgcaac tggctgtccg gcgcatggaa cagcacgctg ttcatgccga
     4621 ccagatgccc ctgcttggtc acgaaccagc cgcccttcgg cagctgcgtg cgttgagcat
     4681 aggcctgttc cacatccgcc acggcataca catgggtcag ccagtcgcgc atcgcgggca
     4741 gcacctgcgc atcctgacac tgcacgtagt tcagcaacgg cgtcaattgc ggcggtgacg
     4801 catcggccga tgcagcctcg gcgttcggtg caaacagcgc caacttggcc ggtggcgcct
     4861 cgctccaagc tgcggcatcg gcaagctgcg gcatggcgat cgcgttgagg cgttcgcgca
     4921 acaccgcctc cagcgcatcc tcccagccct ctccgatgct gatggactgc cacaggcgcg
     4981 gcagccggtc gagctgctgc tgttgcagcc atgccttgag tttctgatcg ttgtccagcc
     5041 gctgctgcaa ctgctgcaag gcgctcaggc gcgcctcggt ctgcgccagc atgcgttcct
     5101 gctgctgcaa cgaggtgcgc tggttgcgcc gctcgttgtc ggccagcggc aattgttcct
     5161 gcagctgcgc cagacgctcc acctgctcgg cgcgctgcat ctcgagcgcg gcctgctctt
     5221 cctgcgcttc ctgcaaggcc gcgtcgtcca cgcgcggcag attgtctttc tcctgcagca
     5281 ggcgctgacg gcgcgactcc agttgctgga tgttttgcag cacatggctg cgctgggtct
     5341 cggccagctg gagtttctgt tgcaactgca tctgttcgcg ctgcagcgcg ttgtagcgct
     5401 cctgcgcctc gcgcgccgca tcctcggcct gcggcaagtt catgctctcg gcttcggcat
     5461 tcatctcggc atcggccagc ttgaccaccg ccgactcctg ctggtgctgc cagtgttcca
     5521 gcagggaatg cacaccctgg cgctgctggc gctgctgttc gatctggcgt tcggtgttgg
     5581 cgatctgctg ggcgacgcgg tggcgctgtt cggcgacatg tttgatctgc tgctccagcc
     5641 gtgcgatttc ggcgttcacg gtatacagct cgccctgccg gacatgcagc gcatcagcca
     5701 gctggtagtg gccgctgcgg gtcgcttcaa gcttgctttc gatctcgcgc aacagcgcgg
     5761 tctcggcttc cagttcgttg cgggtcttct cggtgagctg cacataacgc acgcgctgcg
     5821 attcggcctc ttgccgcttc accagccaga acaattgctg cgtggtgttg cgctgcgcct
     5881 cgaacgcgcg gtactgcttc gccacctcgg cctgctcgcc cagatgcaac aattgtttgt
     5941 ccagttcctg caggatgtcg gacacgcgca acagattgtc gcgcgtgtcg ccgatgcgca
     6001 gctcggtctc gcggcggcgg tcgcggtatt tggacacgcc cgcagcctcc tccagaaaga
     6061 tgcgcaactc ctctggcttg gcctcgatga tgcgcgagat catgccctgc tcgatgatcg
     6121 cgtaggcccg cgcgcctagg ccggtgccga ggaagatatc ggtgatgtcc ttgcgccgca
     6181 catgctggtt gttgatgaag tagctggatt cgccgtcgcg ctgcaacacg cgcttgatcg
     6241 atatctcggc gtagctggac cactgcccgg cagccttgcc cagcgagttg tcgaacacca
     6301 gctcgacgct ggcgcgcgac accggcttgc gcttggaaga gccgttgaag atcacgtcct
     6361 gcatgctgga gccgcgcagt gccgaggcct tggattcgcc cagcacccag cgcagcgcgt
     6421 cgatcacgtt cgacttgccg cagccgttcg ggccgaccac gcccacgatc tgccccggca
     6481 acggaatatg cgtgggatcg acgaaggact tgaatccggc tagcttgatc tgggataaac
     6541 gcaatttggc ttgaacaacg ttttataatg cggatcattc taaccgaagc cgacaggcat
     6601 accaacatga ccacccaacc gaacaagacg ctggaaacct tccccaaccc gaaccccggc
     6661 cgtgactacc atatccacat ggagatcccc gaattcacct gcctgtgccc caagaccggc
     6721 cagcctgact tcgccaccct gttcctggac tacatcccgg accagcgctg cgtggaactc
     6781 aagagcctca agctctacgt ctggtcgttc cgggaggaag gccacttcca cgaggacgtc
     6841 accaaccgca tcc
//
LOCUS       DASUIV010000336         8217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_462579, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000336 DASUIV010000000
VERSION     DASUIV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462579"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1159)
                     /gene="mutY"
                     /locus_tag="VFW59_11900"
     CDS             complement(77..1159)
                     /gene="mutY"
                     /locus_tag="VFW59_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX5364957.1"
                     /translation="MHSFSSRLIRWQRQHGRHGLPWQAADAYRVWLSEIMLQQTQVAT
                     VIPYYQRFIASFPDIEALASATEEQVLAHWSGLGYYARGRNLHRAAQIIAKRYGGEFP
                     QMFEQLVELPGIGRSTAAAICALAYHQRHAILDGNVRRVLSRHRGIEGWAGNKKVEAL
                     LWQQAEALLPNSSPRPAGERVRERGGSNSDIATYTQALMDMGAAVCTRSKPQCTVCPV
                     QADCVASQTDRIAELPSSRPKKAVPERHATFLLLKHGNDILLEKRPGSGIWGGLWCPP
                     QIDDGQGVVADYVRQNGIEANERTDLPAFSHTFTHFKLHITPVLLQLERKPQRVQQAN
                     SVWLDVAEALQAAIPTPVRKLLGQVS"
     gene            complement(1243..3360)
                     /locus_tag="VFW59_11905"
     CDS             complement(1243..3360)
                     /locus_tag="VFW59_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX5364958.1"
                     /translation="MNKILKYGLIGTGAVVGVAVAGAAYLAATFNPNDYKDQIVKAVK
                     DKTQRDLRLDGDITLSFFPNIGANLGRVSLSEFKSEQQFAAIDSAHVSLALLPLLSGK
                     AVVDEVAVSGLQATLIKHKDGTTNIDDLLSPKGEQKDSGQKPIDFDIAAVSIEETALA
                     YRDEGSGAQYAIRDLNLHTGRIANGVPSKIDLSTAVQANQPKLDINTQLKTTLTFDLD
                     KQQYRLEGLDWQVKGAALDIKDLKLQGSGDVSADLSTQEFATQKFVLKASGVQGKDAF
                     EVALDAPQIKVTKDSFSGDQLVLIAKLNGANDITATLTLPSIQGNARAFGSKALTLSV
                     DARGDALPGKQVKSEMKGSFAFDADKQHVEVALAGGLLQSQVKAKVAVNGFSDPAIRF
                     DVDIDQFDADPYLPKKNAEAAKANEPEQPLDLSALRKLNLEGSLRIGSLKVANVKSSN
                     VRVDVKAHNGLLNVAPLSANLYQGSVNGNISVNAQSTPVIAVNQTLSGVNVAALSKDA
                     ADFDTLEGRGNIGMNLTMRGDTVSAMKKALNGTMSLNLADGAIKGINIAKQLRGAQGM
                     LGGGGAQTQSANKDEKTDFSELKASFRVNNGVAHNEDLSMKSPLLRLAGNGDIDIGNS
                     SINYLAKATLAKTLEGQGGKDAVGGITVPVRLTGPFSNLKYTLDFGAMVGEAAKQKID
                     AKKEEVKTKVQEQLKDSLKDLFR"
     gene            complement(3447..4841)
                     /gene="hemN"
                     /locus_tag="VFW59_11910"
     CDS             complement(3447..4841)
                     /gene="hemN"
                     /locus_tag="VFW59_11910"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX5364959.1"
                     /translation="MNSAVNQLIVDLELIRRLDKNGPRYTSYPTADRFTDAFNAERYA
                     YWVAQRQKEGAARPLSLYIHIPFCNTLCFYCACNKVITKDRSKSAEYVRYLIKEMALQ
                     ARLLGAGQVVEQLHFGGGTPTFLSDDEIREVMAAIGQNFKLIEDGEYSIEIDPRKVSD
                     ETIALLGQTGFNRISIGVQDFDDEVQRAVNRIQSEEETVRVITAARANGFKSVSIDLI
                     YGLPKQTLEGFGRTLDRVIATNPDRLSIYNYAHMPTLFKPQRRIHDEDLPTGQLKLDI
                     LSMAVNKLTAAGYVYIGMDHFAKPEDELAVAQRQGLLHRNFQGYSTHADCDLVALGVS
                     AIGKIGPTYSQNYRELEPYYEALDRDQLPIMRGMELNEDDLIRREIIQALMCHFELDK
                     QAFGREFKIDFDQYFATEQDELNEYEREGLLELSPQRIVVTPKGRMLIRNICMVFDKY
                     LRTRTEHARYSKVI"
     gene            5141..5644
                     /locus_tag="VFW59_11915"
     CDS             5141..5644
                     /locus_tag="VFW59_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364960.1"
                     /translation="MELQTTEVERKRARHLHAALLFNLVVNHIFLFLMALTLIKMSNI
                     PMELMVVLSVSILGYILFNAKRSLGSEPSWFVRCHWQYAAKRARMFLILFLVLGTISI
                     AFIYSGHAMGMSSVAIKAFASILGLVFMVSVLGLVVIEFDAEHQAKNGKVPAGAIAYH
                     PYPKENE"
     gene            5649..6326
                     /locus_tag="VFW59_11920"
     CDS             5649..6326
                     /locus_tag="VFW59_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364961.1"
                     /translation="MDKKIILGVIALAIVSMSLLLLAPDNSVHTPDTLPWKISHPTPD
                     TSRVLGITLGQTTLDEAAQAFKNEAEVEISLFKPANGPMGVEAFFEEVNFNGLKAKIV
                     LTINLAPEVLEGMFQRGLRLNSTPSGKRVTLTHDDELKVRQTPISSMAYLPHVRLEEN
                     IFAKRFGEPAERVRETKSGVTHWLYPQLGLDIAVSDKEKPILQYVAPKDFAQLRAPLL
                     ANGEILK"
     gene            complement(6331..6546)
                     /gene="ccoS"
                     /locus_tag="VFW59_11925"
     CDS             complement(6331..6546)
                     /gene="ccoS"
                     /locus_tag="VFW59_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX5364962.1"
                     /translation="MEVIYSLIPGMTFMGLVFVAILIWAVKKGQYEDMEGNASRILLD
                     DDEDAMLISEPKKKAPQGKSPAAPDQQ"
     gene            complement(6552..>8217)
                     /locus_tag="VFW59_11930"
     CDS             complement(6552..>8217)
                     /locus_tag="VFW59_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5364963.1"
                     /translation="SLYVTIAGTGAVYFDTVTNLTFVILIGRYLEGMFRHQAVAATSR
                     LMDLQPRVATVVREGEEKTISVRAVQVGDRVLVKPGNKVPMDGLVVDGRSSVDESMLS
                     GESMPLQKQVGDRVVAGTMNGSGALVVEVQAVAQDSMLAKIIRLVEEAQSSKAPIQRI
                     ADSIVPWFVLATLICASFTFYLWSENFELALMAATSVLIITCPCALGMATPMSIAVAS
                     GLGAKHGILVKNGVVLETLSKVTHFVFDKTGTLTEGRMRVEHVVVAAGQDEAALLTLA
                     ASVERYSEHGVAQAITKAADERQLAYRAMTVTGFHATAGAGVEAEAGGRQVLLGTADW
                     LMRHQVAMQDSLLHKLAEFEGQAKTCVHLAVDGQHVAMLAVADTLRADALGLVNTLRE
                     AGIGMTLLSGDRRPVAEAIARQLGGMEVIAEVLPQDKDRVIQQLQQRGAVVAMVGDGV
                     NDAPALIRADVGIALGSGTDVSVESADIVLMQNELDKVRLATLLSRRTLRTIKQNIGL
                     SFVYNGIMVPLAMMAHVTPLVAAITMPLSSLLVIGNAARIRNLFKE"
BASE COUNT         1609 a         2594 c         2279 g         1735 t
ORIGIN      
        1 tctgcagcat ccgcttcgcc aatcagtcag cccaatagga tcgcatctgg ccgacttcca
       61 atcctcgccc ccttggctat gacacttgcc cgagcagttt ccgcaccggc gtagggatcg
      121 ccgcctgcaa tgcctctgcg acatccagcc acacactgtt agcctgttgc acccgttgcg
      181 gcttgcgctc cagctgcagc agcacaggcg tgatgtgcaa cttgaaatga gtgaaggtgt
      241 gagagaacgc aggcaggtcg gtacgctcgt tcgcctcgat gccattctgc cgcacataat
      301 ccgccaccac cccctgcccg tcgtcgatct ggggcgggca ccacagcccg ccccagatgc
      361 cgcttccggg gcgtttctcc agcagaatgt cgttgccatg cttcagcaat aaaaaggtgg
      421 cgtgccgttc cggcacggcc ttcttcgggc gcgaggaagg gagctcggcg atgcggtcgg
      481 tctgcgacgc aacgcaatcg gcctgcaccg gacagacggt gcattgcggc ttgctgcgtg
      541 tgcagacggc cgcccccatg tccatcagcg cttgggtata ggtggcgatg tcgctattgg
      601 aaccgcccct ctccctaacc ctctcccctg cggggcgagg ggatgaattt ggcagcaatg
      661 cttctgcctg ttgccagagc agcgcttcga ccttcttgtt gccagcccag ccttcgatgc
      721 cgcgatggcg cgacagcacg cgcctgacgt tgccgtccag aatggcgtga cgctgatgat
      781 aggccagcgc acagatggcg gcggcggtgg agcggcctat gccaggcagt tcgacgagct
      841 gctcgaacat ctgcgggaat tcaccgccat agcgtttagc gatgatctgt gcggcacggt
      901 gcaggttgcg gccacgggcg taataaccca gaccgctcca gtgcgccagc acctgctctt
      961 cggtcgcaga agcaagcgct tcgatgtccg gaaaggaggc gataaagcgc tggtaatagg
     1021 ggatgacggt ggcaacctga gtctgttgca gcatgatctc ggacagccac acgcgatagg
     1081 catccgcagc ctgccagggc aggccgtgac ggccatgctg gcgctgccag cgaatcaaac
     1141 gcgaagagaa gctatgcatg cgaatctgcg atcgctcgcg cctcaaacag ccccccgatc
     1201 ggcgggcata gccgaacgat cagcacgaac ggatcttgct catcagcgga acaaatcctt
     1261 gaggctatcc ttcaactgct cctgcacctt ggtcttcact tcctctttct tggcatcgat
     1321 cttttgcttg gcggcctcgc ctaccatcgc gccgaaatcc agggtgtatt taagattgct
     1381 gaacgggccg gtgaggcgca ccggtacggt gatgccgccg acggcatcct tgccgccctg
     1441 cccctccagc gtcttggcca gcgtggcctt ggcgagatag ttgatgctgc tgttgccgat
     1501 gtcgatgtcg ccattccccg cgaggcgcag cagcggggat ttcatcgaca ggtcttcgtt
     1561 gtgcgcgaca ccgttgttca cgcggaagct ggccttgagt tcgctgaaat cggtcttctc
     1621 gtccttattg gccgattgag tctgagcgcc gcccccgccc aacatgccct gcgcaccccg
     1681 caactgcttg gcgatgttga tccccttgat cgcgccgtcg gccagattca gcgacatcgt
     1741 gccgttcagc gccttcttca tcgcgctgac ggtatcgccg cgcatggtga gattcatccc
     1801 gatattgccg cgaccttcca gcgtatcgaa atccgccgcg tccttgctca acgcggcgac
     1861 gttgacgcca ctcagcgtct ggttgacggc gatcaccggt gtggactggg cattgacact
     1921 gatattgccg ttcacgctcc cctgatacag attggccgac agcggtgcga cattgagcag
     1981 gccgttatgc gccttgacgt ccacccgcac attgctggat ttcacattcg ccactttcag
     2041 cgagccgatg cgcagactgc cctccagatt gagtttgcgc agtgcggaca gatcgagcgg
     2101 ctgctcgggc tcattggctt tcgcggcttc ggcattcttt ttcggcagat agggatcggc
     2161 atcgaactga tcgatatcca cgtcgaaccg gatcgcggga tcggagaaac cgttcaccgc
     2221 gaccttggct ttcacctgac tctgcaacag acctcccgcc agcgccacct cgacatgctg
     2281 cttgtccgcg tcgaacgcga agctgccttt catttcgctc ttgacctgct tgccgggcaa
     2341 ggcatcgccc cgtgcgtcca cggacagggt cagcgccttg ctgccaaacg cacgggcatt
     2401 accctgaatc gacggcaagg tcagcgtagc ggtgatgtcg ttggcaccgt tcagtttggc
     2461 aatcagcacc aactggtcgc cgctgaaact gtccttggtc accttgatct gcggcgcatc
     2521 cagcgccacc tcgaaggcat ccttgccctg cacgccgctc gccttcagca caaatttctg
     2581 ggtagcgaat tcctgcgtgg acaggtccgc gctcacgtcg ccgctgccct gcagcttgag
     2641 atccttaatg tccagtgccg cgcctttaac ctgccaatcc aggccttcca gacgatattg
     2701 ctgcttgtcc agatcgaagg tcagcgtggt cttgagctgg gtattgatgt ccagtttggg
     2761 ctggttggcc tgtacggccg tcgacagatc gatcttgctc ggcacgccgt tggcgatgcg
     2821 tccggtgtgc agattcaaat ccctgatcgc atattgcgca cccgagcctt cgtcgcgata
     2881 agcgagcgcg gtctcttcga tgctgactgc ggcgatgtcg aaatcgatcg gcttctggcc
     2941 gctgtctttc tgctctccct ttggggacag caggtcgtcg atgttggtcg tgccatcctt
     3001 gtgcttgatc agcgtggcct gcaagccgct gaccgcgacc tcgtcgacga ccgccttgcc
     3061 ggagagcagc ggcagcagcg cgagcgacac atgcgccgag tcgatggcgg cgaactgctg
     3121 ctcgcttttg aattcggaca gcgacacacg gcccagattc gcccctatgt tggggaagaa
     3181 cgacagcgtg atatcgccgt cgagacgcag gtcacgctgc gtcttgtcct tcaccgcctt
     3241 gacgatctga tccttgtaat cgttcgggtt gaaggtggca gccagatagg ccgcacccgc
     3301 gacggcgacg ccgactaccg cacccgtgcc tatcagcccg tacttgagta tcttgttcat
     3361 gatcgccccg cgataaaaga cagggggaga ttatagcgga gccggaggga agcgcgatgt
     3421 cccctccgtt catccggcca aggtggtcag atcaccttgg agtagcgggc gtgctcggtg
     3481 cgggtacgca gatacttgtc gaacaccatg cagatgttgc ggatcagcat gcgccccttg
     3541 ggcgtgacga cgatgcgctg aggtgacaac tccagcagcc cttcgcgctc gtactcgttc
     3601 aattcgtcct gctcggtggc gaaatactga tcgaaatcga tcttgaactc gcgaccaaac
     3661 gcctgcttgt cgagctcgaa gtgacacatc agcgcctgaa tgatttcgcg tctgatcaga
     3721 tcgtcttcgt tcaactccat gccgcgcatg atgggcaact ggtcgcgatc cagcgcttcg
     3781 taatacggct cgagttcgcg atagttctgg ctgtaggtcg ggccgatctt gccgatggca
     3841 ctcactccca gcgcaaccag gtcgcaatcg gcatgggtgg agtagccctg gaagttgcga
     3901 tgcaacagcc cctgacgctg tgcgacggcc agctcgtctt ccggcttggc gaaatgatcc
     3961 atgccgatgt acacatagcc tgcagcagtc aatttattca ccgccatgct gaggatgtcc
     4021 agcttcaact gtcccgtcgg caggtcttca tcatgaatgc ggcgctgcgg cttgaaaagg
     4081 gtcggcatgt gcgcatagtt atagatcgac aggcgatccg gattcgtagc gatgacccga
     4141 tccagcgtcc tgccaaaacc ttcgagcgtc tgcttgggca ggccgtagat cagatcgatg
     4201 ctgaccgact tgaaaccgtt ggcgcgggct gccgtgatga cgcgcacggt ctcttcctcg
     4261 ctctggatac ggttaacggc acgctgcacc tcgtcgtcaa agtcctgcac accgatgctg
     4321 atgcggttga agccggtctg cccgagcaat gcgatggtct cgtcgctgac cttgcgcgga
     4381 tcgatctcga tggaatattc gccatcctcg atcagcttga agttctgacc gatcgccgcc
     4441 atcacttcgc gtatctcgtc gtcgctgagg aaggtcggcg taccgccgcc gaagtgcaat
     4501 tgctcgacca cctgaccggc ccctagcaac cgggcctgca gcgccatttc cttgatcagg
     4561 tagcgcacat attcggcgga cttgctgcga tccttggtga tgaccttgtt gcaggcacag
     4621 tagaagcaca gcgtgttgca aaacgggata tggatataca aggacagcgg gcgggcagcg
     4681 ccctctttct ggcgctgcgc cacccaatag gcgtagcgct cggcgttgaa ggcgtcggtg
     4741 aagcggtcgg cggtggggta ggaagtgtaa cgcgggccat tcttgtccag cctgcggatc
     4801 aactccagat cgacgatcaa ttgattaact gcgctattca tcacactccc ctgaaagacg
     4861 cgtcccacac cgatatacgg gcagtcctgt cttgataata attttaaaat cggcgggcat
     4921 tataccccat cgccccccgc ccataagggg ccgcacagct gagcccaacg gcttttatgg
     4981 atggttcttt tcagaacacc atcatgcacg gcaccggaag acttctgcac gctcttttcc
     5041 tggtttctcc tgctgttcat caaggactcg caaccgtttg acagtaggca ggcatgagcg
     5101 gattattcgc cacctgattt aattaccgga gaccaaaacg atggaacttc aaaccactga
     5161 agtggaaaga aagcgcgcgc gtcatttaca tgcggcattg ctattcaacc tggtcgtcaa
     5221 ccatattttc ctgtttttga tggccttgac gctcattaag atgagcaata tcccgatgga
     5281 gctcatggtg gtgctttccg taagcatcct cggctacatc ctgttcaatg ccaaacgttc
     5341 gcttggcagc gagccttcct ggttcgtgcg ctgccactgg caatatgccg ccaaacgtgc
     5401 ccgcatgttc ctcatccttt ttttagtgct gggaaccatc agcatcgcgt tcatctacag
     5461 cggacacgcg atgggcatgt cctctgttgc catcaaggcc ttcgccagta ttctggggct
     5521 ggtattcatg gtgtcggtat tgggactcgt cgtcattgaa ttcgacgccg agcatcaggc
     5581 aaagaatggc aaagttcccg ctggcgctat cgcttatcac ccctacccca aagaaaacga
     5641 ataacagcat ggataagaaa atcattctgg gcgtcattgc cctcgccatc gtctcgatgt
     5701 cactgctgct gctggcaccc gacaattcgg ttcacacacc cgataccttg ccatggaaaa
     5761 taagccatcc gacgccggat accagccgcg ttcttggcat caccctgggg caaaccacac
     5821 tcgatgaagc cgcccaggct ttcaagaacg aagccgaagt cgaaatcagc ctgttcaaac
     5881 ctgccaatgg ccccatgggc gtcgaggcat tctttgagga agtcaacttc aatggcctga
     5941 aggcgaagat cgtgctgacg atcaatctgg cgccggaagt gctggaaggc atgtttcagc
     6001 gcggattgcg cctgaacagc accccaagcg gcaaacgcgt cacgctgacc catgacgacg
     6061 aactgaaggt gcgtcagacg ccgatcagca gcatggccta cctgccccat gtgcgtcttg
     6121 aagaaaatat ctttgccaag agattcggcg aaccggcgga gcgagtgcgc gagaccaaaa
     6181 gcggcgtcac tcactggcta tatccccagc tcggcctcga tatcgcggtg agcgacaagg
     6241 aaaaaccgat attgcagtat gtcgctccca aggactttgc gcagttgcgc gcgccgttac
     6301 tggcgaacgg cgaaattctg aagtagcttt ttactgctga tcgggcgctg cgggagactt
     6361 tccctgcggc gccttttttt tgggctcgct gatcagcatc gcatcttcat cgtcatccag
     6421 cagaatgcgg ctggcattgc cttccatatc ttcgtattgc cccttcttaa ccgcccagat
     6481 caggatggcg acgaacacca gccccatgaa cgtcatgccg ggtatgagac tgtagatgac
     6541 ttccatgatt gctattcctt aaacaggtta cgaatacgtg ccgcattgcc gatcaccagc
     6601 aatgagctca gaggcatggt gatcgccgcc accaatggcg tgacatgcgc catcatcgcc
     6661 agcggtacca tgatgccgtt atacacgaac gacaatccga tattctgctt gatggtgcgc
     6721 agtgtccggc gcgacagcaa ggttgccaga cgcaccttat ccagctcgtt ttgcatcagc
     6781 acgatgtccg cgctctccac cgagacatcg gtgccggagc ccagcgcgat gccgacatcg
     6841 gcacggatca gcgcgggtgc atcattgacg ccatcgccca ccatcgccac cacggcgccg
     6901 cgctgctgca gctgctgaat cacccggtcc ttgtcttgcg gcagcacctc ggcgatgacc
     6961 tccatgccgc ccaattggcg cgcgatcgcc tcggccaccg gcctgcgatc cccgctcagc
     7021 agggtcatgc cgataccggc ctcgcgcagg gtattcacca accccagcgc gtcggcgcgc
     7081 agggtatccg ccaccgccag catggcgaca tgctggccgt ccacggccag atgcacgcaa
     7141 gtcttggcct gcccctcgaa ctcggccagc ttatgcagca agctgtcctg catcgcgacc
     7201 tggtgccgca tcaaccaatc ggccgtcccg agcaacactt gccggcctcc ggcctcagcc
     7261 tcgacacccg cacccgctgt cgcatggaac cccgttaccg tcatggcacg ataggccaac
     7321 tggcgctcgt ctgccgcttt ggtaatggcc tgcgccacac cgtgctccga atagcgctct
     7381 acgcttgcgg ccaaggtcag cagcgccgcc tcatcctgcc cggctgccac gaccacatgt
     7441 tcaacgcgca tcctgccttc cgtcagtgtg ccggtcttgt cgaatacgaa gtgcgtcacc
     7501 ttggacaagg tctccagcac cacaccgttc ttcaccagaa tgccatgttt ggctcccaac
     7561 ccggatgcca cggcgatcga catcggcgtg gccataccca gcgcacaggg acaggtaatg
     7621 atcagcaccg aggtagccgc catcagcgcc aactcgaaat tttcagacca caggtagaaa
     7681 gtgaagcttg cgcatatcag cgtagccagc acgaaccagg gcacgatact gtcggctatc
     7741 cgctggatcg gcgccttcga ggactgcgcc tcttccacca gccggatgat cttagccagc
     7801 atggaatcct gcgcaacggc ctgcacctcg acgaccaggg cgccgctgcc gttcatcgtg
     7861 cccgccacga cccggtcacc gacttgcttc tggagcggca tcgattcgcc gctcagcatc
     7921 gactcgtcca ccgaactgcg cccatcgacc accagaccgt ccatcggaac cttgttgccg
     7981 ggtttgacca gcacgcgatc acccacctgc accgcacgca ccgagatcgt tttctcctcg
     8041 ccctcgcgca cgacagtcgc cacacgcggc tgcaaatcca tcaggcgact ggtcgccgcc
     8101 acggcctgat ggcggaacat gccctcgagg tagcgcccga tcaaaatcac gaaggtcaga
     8161 ttggtgaccg tatcgaaata caccgccccc gttccggcga tggtcacata cagggaa
//
LOCUS       DASUIV010000337         7807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1112614, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000337 DASUIV010000000
VERSION     DASUIV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112614"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFW59_11935"
     CDS             complement(<1..786)
                     /locus_tag="VFW59_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5364964.1"
                     /translation="MRFDKFTTKLQQALSDAQSLAVGADNQFIEPQHLLLALLNDADS
                     GAGSLLARAGGNSNALKTALNDAVSRLPKVEGHGGEVQVGRDLSNLFNVTDKEAQKRG
                     DQFIASEMFLLALTGDKGEAGRLLKQHGVQRAPLEQAINAVRGGESVGSQEAEGQRES
                     LKKFTIDLTERARQGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLKRKKVLSLDMAALVAGAKYRGEFEERLKNV"
     gene            912..1751
                     /gene="nadC"
                     /locus_tag="VFW59_11940"
     CDS             912..1751
                     /gene="nadC"
                     /locus_tag="VFW59_11940"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5364965.1"
                     /translation="MALTTLALDIQSSVRAALEEDVGDGDLTAQLIPADKVSTATIIT
                     RQQAVLCGSPWLEECFHTLDPDCDIRWAAQEGELLQPGQTLCTITGNARALLTAERCA
                     LNFLQTLSATATATRRYVEIVAGTRAKILDTRKTLPGLRLAQKYAVTVGGGHNQRVGL
                     YDGVLIKENHIAAAGGIRAVLDQAFRIAPAGVSIQIEVESLDELREALDAGAKLILLD
                     NFTLNMMREAVALTAGRAELEASGGVNLDTVRAIAETGVDRISIGALTKDIQAVDLSM
                     RIT"
     gene            1950..2762
                     /locus_tag="VFW59_11945"
     CDS             1950..2762
                     /locus_tag="VFW59_11945"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX5364966.1"
                     /translation="MCQLLGMNCNTPTDICFSFTGFQKRGGETDVHADGWGIAFFEGR
                     GVRLFLDPQPSAQSPVAELVRNYPIRSLNVIAHIRKATQGVVSLENTHPFQRELWGRY
                     WVFAHNGNLPQFLPDLDGSFLPVGNTDSERIFCWLLQSLRQRFCDAPPDRDAVFAALH
                     ELTLPLAAMGIFNFLLSNGECLFAHCSTELSYVVRRAPFDVAHLKDEDVMVDFSEVTT
                     PNDRVAIIATLPLTDNEPWITMPPGSLWLFHDGDAVTHRPTAPSPVKSLSGN"
     gene            2984..3418
                     /gene="trxC"
                     /locus_tag="VFW59_11950"
     CDS             2984..3418
                     /gene="trxC"
                     /locus_tag="VFW59_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEX5364967.1"
                     /translation="MTESLHIVCPHCDAVNRVPAEKLKAQPTCGKCKQALFTAHPIEL
                     TAANFDRHLTRNDIPLLVDFWAPWCGPCRMMAPAFEKAAQQLEPGFRVAKLNTEAEQM
                     LAGRYAIRSIPTLALFRGGREIARQAGAMDAAGIVHWARAQG"
     gene            complement(3434..6493)
                     /locus_tag="VFW59_11955"
     CDS             complement(3434..6493)
                     /locus_tag="VFW59_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5364968.1"
                     /translation="MNVSSWSIRNPLPAILAFVLLSFIGVMAFKAMKIQLFPDIDLPM
                     ITVTATMPGASPDQMETEVARKIENALAGAQGLKHLYTQVKDGSVTVSAEFRLERDSR
                     LALEDVRSAISRIRSELPREMLDPIINKVEMSGKPILTYTITAPRMSEEELSWFVDNN
                     VTKLLLGVKGVGGVNRVGGVTRQVRVELDPSKLMALNLTAAELSGQLRQIQQEAPGGR
                     TDLGGMEQSVRTVATVQTAAQLGQMEITLRDGRRVRLDQVADVSDSIAERRTAALHNG
                     KQVVGFEIMRSLGAGDVAVGDGVARALEGLKQTHPEISVEKVFNTVDQVYETYQGSME
                     MIFEGALLAVIVVIFFLRNTRATIVAATALPLAVIPTFAFMYLLGFTINIVTLLSLSL
                     VVGVLVDDAIVEIENIMRHLEMGKTPYQAAMEAADEIGMAVIATTFTLVAVFLPTAFM
                     NGIPGRFFVQFGWTAAIAVLFSLVVARMLTPMMSAYFLRPRVAHPTPGWIAVYLKWAQ
                     WCLAHRKTTLAVTAVFFFGSFMLAGTLPTAFQPKDNLSQTQITLTLPPGSRFEQTYAL
                     AEQARAIVMKNPNVTLVYTAIGGGATGGNVFEPAGAAEARKATLSIQLKPRDQRAVKQ
                     QIEGSLRDALAVLPGVRVKVGMGSSSDKYQLVLTSENGKLLNEHALKVGQELRTIPGI
                     GNVASNSSILRPEVVVRPDFARAADLGVTSSAIAETLRVATNGDYDQALAKLNLPDRQ
                     LPIIVKLRDDARQDIELLSRLTVQGSHGPVMLGSVATLSLGSGPAQIDRYDRQRNITF
                     DVELAGQPLGKVQAAALALPSMKSMPAGVEMAPIGDAEMMKELFASFGLAMLTGVLCI
                     YIVLVLLFKDFMQPATILGALVLSIPGAFLALFLTHTAISMPSMIGLIMLMGIATKNS
                     ILLVEYAVVSRRDRGMDRAEALLDACRKRVRPIVMTTMAMGAGMLPVALDMGTGDGSF
                     RAPMAIVVIGGLITSTFLSLLVIPVVFTYVDDLVQWLRKGFARRSRA"
     gene            complement(6494..7456)
                     /locus_tag="VFW59_11960"
     CDS             complement(6494..7456)
                     /locus_tag="VFW59_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5364969.1"
                     /translation="MALSFLTPNRGRWLVGGVVVFLALAGCGRAPADKAAKEPAVAKP
                     ALTVSLTTLQPAQWERTLAANGGVAAWQEAIITAQLPGLRISEVKVGIGDHVKQGDVL
                     VTLGNLTRAGEDYVQGRVIAPDDGVISAANASVGSMVQSGAELFRLIRKGRLEWRAEL
                     TAEELILLRKGMPVEVSAGEGRIVKGRVRAISPSVNAQTRYGYALVDLPNDSGMIAGM
                     FARGSFQLEQPKPSWVLPQSAVMLRDSQAYVFMVGADSRVQERRVGIGSRRGEQIEIV
                     AGLEQGESVVESGGAFLVAGDTVSVVPGSSPLPQAGEGANAGSR"
     gene            complement(7511..>7807)
                     /locus_tag="VFW59_11965"
     CDS             complement(7511..>7807)
                     /locus_tag="VFW59_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5364970.1"
                     /translation="AIFIQFAPPGLREQMFAHIERSLKPGGLLLLQGYTPRQLEYRTG
                     GPSQVENLYTEALLREAFVGMEILHLAEHDSVISEGAGHRGMSALIDLVARKIL"
BASE COUNT         1459 a         2669 c         2344 g         1335 t
ORIGIN      
        1 cacgtttttc agacgctcct cgaactcgcc gcgatacttc gcacccgcca ccagtgcggc
       61 catgtccagg ctcagcacct tcttgcgctt cagagattcc ggcacttcac cgttgacaat
      121 gcgctgcgcc agcccttcga cgatggcggt cttgcccacg ccgggttcgc cgatcagcac
      181 cgggttgttc ttggtgcggc gctgcagcac ctgaatcgcg cggcggatct cgtcgtcgcg
      241 gccgatcacc ggatcgagct tgccctgccg cgcgcgttcg gtcagatcga tagtgaattt
      301 cttcagcgac tcgcgctggc cttcggcctc ctgcgagccc accgactcgc cgccgcgcac
      361 cgcgttgatc gcctgctcca gcggcgcacg ctgcacgccg tgctgcttca gcagccgccc
      421 ggcctcgccc ttgtcgccgg tcagcgccag caggaacatc tcggacgcga tgaactggtc
      481 gccgcgtttc tgcgcttcct tgtcggtcac gttgaacagg ttggacaggt cgcgtccgac
      541 ctgaacctcg ccgccgtgac cttccacctt gggcagacgt gacacggcgt cattcagcgc
      601 ggtcttcagc gcattgctgt tgccgcccgc ccgcgccagc agcgaacccg cgccgctgtc
      661 ggcgtcgttg agcagcgcca gcagcaggtg ctgcggctcg atgaactggt tgtcggctcc
      721 gaccgcgagg ctctgggcat cggacagggc ttgttgcagc ttggtcgtga acttgtcgaa
      781 acgcatggtc aagcactcct tctgatgaat tcgaatggac actaggtggg gcgccgtgcg
      841 gaaatttcaa gtccacgaat accagcctcg cgccatcctc tatactgcac ccatctgaac
      901 aggaatgcac catggctctc actaccctag ctctcgatat ccaatccagt gtccgcgccg
      961 ccctcgaaga ggacgtcggc gacggcgacc tgaccgcaca gctgattccg gccgacaagg
     1021 tctccaccgc gaccatcatc acgcgccagc aggccgtgct gtgcggctcg ccctggctgg
     1081 aggaatgttt ccacaccctc gatcccgact gcgacatccg ctgggcggcg caggaaggcg
     1141 agctgctgca gcccggacaa acgctctgca ccatcacggg caacgcgcgt gcgctgctga
     1201 cggcggagcg ctgcgcgctg aactttttgc agacattatc cgcgaccgct acggcgacgc
     1261 gacgttacgt ggagatcgtg gcgggcaccc gcgcgaagat actggacacg cgcaagaccc
     1321 tgcccggcct gcgccttgcg cagaagtacg cggtcaccgt cggcggcgga cacaaccagc
     1381 gcgtcgggct gtatgacgga gtgctgatca aggaaaacca catcgccgcc gcaggaggca
     1441 tccgcgcggt gctggatcag gcgttccgca tcgccccggc aggcgtctcc atccagatcg
     1501 aggtggagag tctggacgag ctgcgcgagg cactggatgc gggtgcgaaa ctgatcctgc
     1561 tcgacaactt cacgctgaac atgatgcgcg aggcggtggc gctgaccgca ggccgcgccg
     1621 aactcgaagc ctcgggcggc gtgaacctcg acaccgtgcg agccatcgcc gagaccggcg
     1681 tggatcgcat ctccatcggc gcgctgacca aggacataca ggcagtagac ctgtcgatgc
     1741 gcataacctg acacaccctc tcccgcgagc gggagagggg ttgggggaga gggcaaggag
     1801 aatccgggca cctctagaaa tcgtcgcgag caagccggag tgcaaggagc cgcgcagtga
     1861 atgacgagac ataccgcatg ggtaggcgag gaatgaacga gcacgtgacg cagcaatctg
     1921 gctgcgcagt agatttatag aggtgtccta tgtgccaatt gctagggatg aactgcaaca
     1981 cccccaccga catctgcttt tccttcaccg gattccagaa gcgcggcgga gagaccgacg
     2041 tgcatgccga cggctggggc atcgccttct tcgagggacg cggcgtgcgg ctgttcctcg
     2101 atccgcagcc ctcggcgcag tcgcccgtcg ccgagctggt gcgcaactac ccgatccgct
     2161 cgctgaacgt catcgcccat atccgcaagg ccacgcaagg cgtggtgtcg ctggagaaca
     2221 cccatccctt ccagagagag ctatggggac gctactgggt gttcgcgcac aacggcaacc
     2281 tgccgcagtt tctgcccgat ctggacggca gctttctgcc ggtgggcaac accgacagcg
     2341 agcgcatctt ctgctggctg ctgcagagcc tgcgccagcg tttttgcgat gcgccgcccg
     2401 accgcgacgc ggtgttcgcg gcgctgcatg aactcaccct gccgctggcc gcgatgggca
     2461 tattcaactt cctgctgtcc aacggcgaat gcctgttcgc ccactgctcc accgagctgt
     2521 cctatgtcgt gcgccgcgca cccttcgacg tggcgcacct gaaggacgag gacgtgatgg
     2581 tggacttcag cgaggtgacc acgccgaacg accgagtcgc catcatcgcc accctgccgc
     2641 tgaccgacaa cgaaccctgg atcaccatgc cgcccggcag cctgtggctg ttccacgacg
     2701 gcgatgcggt gacccaccgc ccgaccgccc ccagcccggt gaaatctctt tcgggcaatt
     2761 gaattctgcc gctgcacccc caagtggggt aagcgaaacc aatgcaacca tttgtccacg
     2821 aaagacacga aaagcactaa aaaaacccgc atgcatctcg ccattggata aaaatatcgg
     2881 cattgagatc aatctggagc gatagaaagc tctattcgcc ccgtttcgtg cttttcgtgt
     2941 ctttcgtgga tcaatagatt tttacagaat gaaaggaaca cccatgaccg aatccctgca
     3001 tatcgtctgc ccgcactgcg acgcggtgaa ccgcgtaccg gcggagaagc tgaaggcgca
     3061 acccacctgc ggcaagtgca agcaggcgct gttcaccgcc caccccatcg aactcactgc
     3121 ggccaatttc gaccgccacc tgacgcgcaa cgacatcccg ctgctggtgg atttctgggc
     3181 gccctggtgc gggccctgcc ggatgatggc gcccgccttc gaaaaagcgg cacaacaact
     3241 ggagccggga ttccgcgtgg ccaagctgaa taccgaggcc gagcagatgc tggccggacg
     3301 ctacgccatc cgcagcatcc cgacgctggc gctgttcagg ggagggcgcg agatcgcccg
     3361 gcaggcgggc gcgatggatg cggcgggcat cgtgcattgg gccagagcac agggctagag
     3421 atgcccggat ggatcaggcg cgagacctgc gcgcaaaacc ttttctcagc cactgcacca
     3481 ggtcgtccac ataggtaaac accaccggaa tcaccagcag gctgaggaag gtggaggtga
     3541 tcagtccgcc gatcacgacg atcgccatgg gtgcccggaa actgccgtcc ccggtaccca
     3601 tgtcgagcgc gaccggcaac atgcccgcac ccatcgccat cgtggtcatc acgatgggac
     3661 gcacgcgctt cctgcaggca tcgagcagcg cctcggcccg gtccatgccg cgatcgcggc
     3721 gcgagacgac ggcgtattcc accagcagga tggagttctt ggtggcgatg cccatcagca
     3781 tgatcaggcc gatcatcgac ggcatcgaga tcgcggtgtg ggtcaaaaac agcgccagga
     3841 acgcgccggg aatggacagc accagcgcgc ccagaatcgt ggcgggctgc atgaaatcct
     3901 tgaacagcag caccagcacg atgtagatgc acagcactcc ggtgagcatc gccagaccga
     3961 agctggcgaa cagctccttc atcatctcgg catcgcctat cggcgccatc tcgacaccgg
     4021 cgggcatgct cttcatggaa ggcaacgcca gcgcggcggc ctgcaccttg cccagcggct
     4081 gtcccgccag ttcgacgtcg aaggtgatgt tgcgctggcg gtcgtagcgg tcgatctgcg
     4141 cgggaccgct gcccagactc agcgtcgcca cgctgcccag catcaccggc ccgtgcgagc
     4201 cctgcaccgt cagacgcgac agcagctcga tatcctgacg cgcgtcgtcg cgcagcttca
     4261 cgatgatcgg cagctggcga tccggcagat tgagtttcgc cagcgcctgg tcgtaatcgc
     4321 cgttggtcgc gacgcgtagc gtctcggcga tcgcgctcga cgtcacgccc agatcggcgg
     4381 cgcgcgcgaa atcggggcgc accaccacct cgggacgcag gatgctggag ttcgaagcca
     4441 cgttgccgat gcccgggatc gtgcgcagct cctgcccgac tttcagcgcg tgctcgttca
     4501 acagcttgcc gttctcgctg gtcagcacca gttgatactt gtcagacgac gaacccatgc
     4561 cgaccttgac gcgcacgccc ggcaacaccg ccagcgcatc gcgcaggctg ccctcgatct
     4621 gctgcttcac ggcgcgctga tcgcgcggct tgagctgaat ggacagcgtg gccttgcgcg
     4681 cctccgccgc tccggccggc tcgaacacat tgccgccggt cgccccgccg ccgatcgcgg
     4741 tgtagaccag cgtcacgttc ggattcttca tcacgatcgc gcgcgcctgt tcggccagcg
     4801 cataggtctg ctcgaatctg ctgcccggcg gcagcgtcag ggtgatctgg gtctgcgaca
     4861 ggttgtcctt gggctggaag gcggtcggca aggttccggc cagcatgaaa gagccgaaga
     4921 agaataccgc cgttaccgcc agcgtagtct tgcgatgcgc cagacaccat tgcgcccatt
     4981 tcagatagac cgcgatccag cccggcgtcg gatgcgcgac gcgcgggcgc aggaaatagg
     5041 cggacatcat cggtgtcagc atccgcgcca ccaccagcga gaacagcacc gcgatcgccg
     5101 ccgtccagcc gaactgcacg aagaagcggc ccgggatgcc gttcatgaag gcagtcggca
     5161 ggaacaccgc caccagcgtg aaggtcgtcg cgatcaccgc catgccgatc tcgtcggccg
     5221 cttccatcgc cgcctgatag ggcgtcttgc ccatctccag atggcgcatg atgttctcga
     5281 tctcgacgat cgcgtcgtcc accagcacgc cgaccaccag cgacagcgac agcaaggtca
     5341 cgatattgat cgtgaaaccc agcagataca tgaaggcgaa ggtgggaatc accgccagcg
     5401 gcagcgcggt cgccgcgacg atggtggcgc gggtgttgcg caggaagaag atcaccacga
     5461 tcaccgccag caacgcgccc tcgaagatca tttccatcga cccctgatag gtctcgtaaa
     5521 cctggtcgac ggtattgaac accttctcga cggagatctc cggatgggtc tgcttcaggc
     5581 cttccagcgc ccgggccaca ccgtcgccca cggcaacatc gcccgcgccc agcgagcgca
     5641 tgatctcgaa accgaccacc tgcttgccgt tatgcaatgc cgccgtgcgc cgctcggcga
     5701 tcgagtcgct gacatccgcc acctgatcca gccgcacgcg gcgtccatcg cgcagggtga
     5761 tctccatctg ccccagttgc gcggccgtct gcaccgtcgc cacggtgcgc acggattgct
     5821 ccatgccgcc cagatcggtg cgtccgcccg gcgcttcctg ctggatctgg cgcaactgtc
     5881 cggacagttc ggcagcggtg agattgagcg ccatcagttt ggacggatcg agttccaccc
     5941 tgacctggcg cgtcaccccg ccgacgcgat tgaccccacc gacccccttc acgccgagca
     6001 gcagcttggt gacgttgttg tccacgaacc aggacaactc ttcctcgctc atgcgcggcg
     6061 ccgtgatggt gtaggtcagg atgggcttgc cgctcatctc caccttgttg atgatcggat
     6121 cgagcatctc gcgcggcaac tcgctgcgta tgcgcgaaat ggccgagcgc acatcttcca
     6181 gcgccagacg cgaatcgcgc tccagccgga attcggcgga gacggtgacg ctgccatcct
     6241 tgacctgggt gtacaggtgc ttgaggccct gcgcgccggc cagcgcattc tcgatcttgc
     6301 gcgccacctc ggtttccatc tggtctggcg atgcgccggg catcgtggcc gtcaccgtga
     6361 tcatcggcag atcgatgtcg gggaagagct ggatcttcat ggccttgaag gccatcaccc
     6421 cgatgaacga cagcagcacg aaggcgagta tcgccggcag cggattgcgg atggaccagg
     6481 aagaaacgtt catctatcga gaccctgcat tcgccccctc tcccgcttgc gggagagggg
     6541 aggaacccgg cacgacgctc accgtgtcgc ccgccaccag aaacgcaccg ccggactcaa
     6601 ccaccgactc gccctgctcc agccccgcga cgatctcgat ctgctcgccg cgacggctgc
     6661 cgatgccgac acggcgttcc tgcacgcgcg aatcggcgcc caccatgaac acataggcct
     6721 ggctgtcgcg caacatcacg gcggattgcg gcaacaccca ggatggcttc ggttgctcca
     6781 gctgaaaact gccgcgcgcg aacatcccgg cgatcattcc ggagtcgttc ggcagatcga
     6841 ccagcgcata accgtaacgg gtctgtgcgt tcaccgaagg cgagatcgcg cgcaccctgc
     6901 ccttgacgat gcgcccctcg cctgcggaga cttccacagg catgcctttg cgcaacagga
     6961 tcagctcttc ggcggtcagt tccgcgcgcc attccagacg ccccttgcgt atcaggcgaa
     7021 acagctcggc gccggactgc accatcgaac ccacgctggc attggccgcc gagatcaccc
     7081 cgtcgtccgg cgcgatgacg cgtccctgca cgtaatcctc gcccgcgcgc gtcagattgc
     7141 ccagcgtcac cagcacatcg ccctgcttca catgatcgcc gatgccgacc ttcacctcgg
     7201 agatgcgcaa tcccggcaac tgggccgtga tgatggcctc ctgccaggcc gcgacaccgc
     7261 cgttcgccgc cagcgtccgc tcccactgtg ccggttgcag cgtggtcagg gacacggtca
     7321 gcgcgggctt ggcgaccgca ggttccttcg cggccttgtc ggcgggcgca cggccgcacc
     7381 cggccagggc aagaaaaacc accactcctc cgaccagcca gcgtcccctg ttcggagtca
     7441 aaaaactcaa agccatgtac ctgttcctat cgacaaaacg ttttgcattc tacgccgcga
     7501 ccaccacccg ctagaggatt ttacgcgcca ccagatcgat cagcgccgac atgccgcgat
     7561 gccccgcgcc ctcgctgatg acgctgtcgt gttctgccag atgcaggatc tccatgccga
     7621 cgaacgcctc gcgcagcaac gcctcggtgt agagattctc gacctgcgac ggtccgccgg
     7681 tccggtattc cagctggcgc ggcgtgtagc cctgcaacag cagcaaaccg cccggtttga
     7741 gactgcgttc gatatgggcg aacatctgct cgcgcaaacc gggcggcgcg aactggatga
     7801 agatcgc
//
LOCUS       DASUIV010000338        10961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_647665, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000338 DASUIV010000000
VERSION     DASUIV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10961
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647665"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1165
                     /locus_tag="VFW59_11970"
     CDS             200..1165
                     /locus_tag="VFW59_11970"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5364971.1"
                     /translation="MKVSFLDFESSIAELEDKIEQLRYVQDDSALDIADEISRLSKKS
                     EALTKDVYSKLTPWQISQVSRHPQRPYTLDYIEHLFTDFEELHGDRSFADDKAIVAGL
                     ARFNGQSVMVIGHQKGRDTKEKITRNFGMPRPEGYRKAMRLMKLAEKFGIPIMTFIDT
                     PGAYPGVGAEERGQSEAIGRNLYEMSELRVPIICTVIGEGGSGGALAIAVGDAMLMLQ
                     YSTYSVISPEGCASILWKSADKAADAAETLGITATRLKALGLVDKIISEPLGGAHRDY
                     AATMQSVKKALQDALKLAQSKPTANLLQDRFNRLMSYGKFKEVEL"
     gene            1265..2617
                     /gene="tilS"
                     /locus_tag="VFW59_11975"
     CDS             1265..2617
                     /gene="tilS"
                     /locus_tag="VFW59_11975"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5364972.1"
                     /translation="MSRHDLVDRVAARLAPLLPPRSTILIGLSGGVDSVVLLHLLHHL
                     APRFDWRLSALHVHHGISPNADAWAQFCTELCAAQAVPLHIERVDIAPLREHGIEAAA
                     RKLRHAAFASQRCDFVALAHHADDQAETLLLQLLRGAGVRGVASMPVLSKVEGPLLGQ
                     AAVVRPLLDVARATLLDYACQHQLRWVEDESNADESYPRNFLRHRALPLLEERFPACR
                     ETLARSARHFAEASELLDDLAQMDAEGAIQGDALNVPVLRSLTPPRAKNLLRHFLQLQ
                     GAPMPQAAQLDDMLHQLCGARQDAAVCVAYGGHWQVRRYQDQTFVSPALGELDLGLVM
                     AWNGEAELSWPALEESLRFDETQGAGISLAKLRRAPVKLCLRHGKERLRPHPRAARRS
                     LKHLLQEQGVPPWRRERLPLLYCGDELVCVVGVAVAAEYQAGPGEAAIVVELAGSAGE
                     "
     gene            complement(2688..3299)
                     /locus_tag="VFW59_11980"
     CDS             complement(2688..3299)
                     /locus_tag="VFW59_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5364973.1"
                     /translation="MNDLFAAVIHDVKNQLAELSLRLGERDDAQQEMVIALNAARRLS
                     EVLLVHRQESDLLSVNQDSVDTADFLAILAAEYRELFPALTFDIDVAQAPAFAFFDDA
                     LVRLALSNALHNACRSAKTRVRLSAREQDAMLILEVADDGPGFPAGMLTNEGGIPAFV
                     SNRGTGLGLYLARKIAELHKLKERHGRIELSNAEGAVFRMFLP"
     gene            complement(3317..4921)
                     /locus_tag="VFW59_11985"
     CDS             complement(3317..4921)
                     /locus_tag="VFW59_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5364974.1"
                     /translation="MATLSEFTDKQVLVIDDMEGMRSQLRMSLSNSGFAKLHVVANIR
                     EALQHMENNRYDVILCDYSLGSGTDGQQFLEHLRTNDLISRNTIFVMITAEQAYEKVV
                     SASECAPDDYLLKPFTAGQFNARLVKLLERQAYFRDLDQATDAKNWPHVIAECDKALP
                     ARNKYFIELCKIKGAALMHHNRPQEAAELYREVLALRPIGWARLGLARALSLLNQKNE
                     AQQLAQEILTDSPQFMAAYDFLGGLLSSSGDKKAALEVLQKAREVAPGTMSRIRELST
                     LAVDTGQPELAEAVMRQALQKHKYSPVRRVNDYAVLSHALANQGKANEALSIIGEARK
                     DFRDEQSQIILATSESVAQHAAGNAELAEAALARAMTGDINALSAETITSIADACFTL
                     GKEQEATGLLRHAIQNNPEDETIRGKVHDVLMAAGKDVTEADAMIEDSTQEIIRINND
                     GVRKAESGQLDEAVALLCEAANRLPNNLQIVGNAALVIALDLVQNGNDPQKLSKCMRY
                     REALIAKSPDYPKIAQIDSQLKQLKP"
     gene            5089..6612
                     /locus_tag="VFW59_11990"
     CDS             5089..6612
                     /locus_tag="VFW59_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX5364975.1"
                     /translation="MTVIRQQDFIDSIADALQFISYYHPTDFIRALAEAYRLEQSPAA
                     RDAMAQILTNSRMCAQGHRPICQDTGIVVAFVRVGMSVRWEADMDIVGMVNAGVRRAY
                     LDSDNVLRASIVSDPAGRRKNTGDNTPAVVHVELVQGDKVEVEIAAKGGGSENKAVME
                     MLNPGDDIVEWVLQRVPEMGAGWCPPGMLGIGIGGTAEKAVLLAKEALMQPMDMHELK
                     ARGPQNRLEELRIELYDKVNALGIGAQGLGGLTTVMDVKILDYPTHAASKPIAIIPNC
                     AATRHVHFELDGSGVATFEPPKLEDYPDVHWHPAEDARRVNLETVTREEISHWQPGET
                     VLLSGKLLTGRDAAHKRIAEMLARGEKLPSGVDFTGRFIYYVGPVDPVRDEVVGPAGP
                     TTATRMDGYTEMMLAQTGLMGMVGKAERGRDAIESIKKHGAVYLIAIGGAAYLVSKAI
                     RSARVVAFPELGMEAIYEFEVENMPVTVAVDVRGRSVHDIGPAEWRKRIGMIPVHAT"
     gene            6620..6718
                     /gene="ffs"
                     /locus_tag="VFW59_11995"
     ncRNA           6620..6718
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFW59_11995"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6743..6991
                     /locus_tag="VFW59_12000"
     CDS             6743..6991
                     /locus_tag="VFW59_12000"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX5364976.1"
                     /translation="MKMKLGWLISALLFSGNLYALGSVSVEYGSALQFGDHLGAGVRL
                     GEHQQFDLALRFQHLSNGDIKQPNHGINLNQVRFASHF"
     gene            7003..8604
                     /gene="dnaX"
                     /locus_tag="VFW59_12005"
     CDS             7003..8604
                     /gene="dnaX"
                     /locus_tag="VFW59_12005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5364977.1"
                     /translation="MSTTTVLARKWRPRNFAQLAGQEHVVKALSNALTQNRLHHAYLF
                     TGTRGVGKTTIARIFAKSLNCEAGITATPCGECSACREIDSGRFVDLIELDAASNTQV
                     DSMRELLESALYAPTSGRFKVYIIDEVHMLSKSAFNAMLKTLEEPPGHVKFILATTDP
                     QKIPVTVLSRCLQFNLKQLPPALIASRLQYVLEQEQLTFDAGALALVARAAQGSMRDA
                     LSLMDQAIAFSSGKVEESVVRTMLGAIDHGYLFEVLAALRDRNGLALIEIADNMASRS
                     VAFDAALQELATLLHRIALAQTVPQAIADDEPERGRLLELAQTLTPEQVQLYYQIAIH
                     GRDEIDLAPDEYAGFSMTLLRMLSFAPARGAAIATVPVSPAVLQAPVAQRPAVSEVAV
                     AEVAPEPRRVAPSLGGALDWGVLLAQLNLQGMAQQLAKHSVLESLSDDLVTLCLSQEQ
                     KHLQTKMAVDRLQAALSEYFAKPVRLNIVLGKAETATPALVEQQTKQLRQQEATDSIE
                     HDVFVRDAQAELDASVLEESIKPIQ"
     gene            8618..8944
                     /locus_tag="VFW59_12010"
     CDS             8618..8944
                     /locus_tag="VFW59_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5364978.1"
                     /translation="MMKGGLGGLMKQAQQMQQNMQKAQAELATVEVEGQAGSGMVKVL
                     MTCGHEVRRVSLDDSVLGDDKEMLEDLIVLALNDAMKKAEATTQQRMGAFTSGMGLPP
                     GVKLPF"
     gene            8944..9540
                     /gene="recR"
                     /locus_tag="VFW59_12015"
     CDS             8944..9540
                     /gene="recR"
                     /locus_tag="VFW59_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5364979.1"
                     /translation="MTSSHLDELIAALRCLPGVGPKSAQRMAYHLLQRDRAGALRLAQ
                     SLELAASNILHCSKCNNFSEHEVCALCASSKRDAEVLCVVEMPADLLMMEQAQCYRGM
                     YFVLMGRLSPLDGVGAKELHFDRLLKRAKEQVREVILATNFTVEGDATAHYLTNLLRT
                     QGIKVSRIARGLPVGGELEQVDSGTLAQAVLERREVMA"
     gene            9537..10475
                     /locus_tag="VFW59_12020"
     CDS             9537..10475
                     /locus_tag="VFW59_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5364980.1"
                     /translation="MIKKNRRNPDELPVESELTEEKDIGERLQKVLAQAGFGSRREME
                     EWIANGRVSVNGEPATLGMRVVAGDLVKTERRTIRVGERDYAARVLLYHKPEGEIVSR
                     DDPEGRTSVFDKLPKLRGQKWIAIGRLDFNTSGLLIFTTSGDLANRLMHPRFEVEREY
                     AVRVQGTMTDEQMTQLLKTGVELEDGLVKFEKLEDEGGEGFNHWYRVMLKEGRNRVVR
                     RSFDALGLPVSRLMRVRFGIINLPPRLKRGMTAELGEGEVAQILEWVGIASDEVEAVA
                     PRPVRNAPAKSDHRPGGAARGGPRRGTGKSKAGAKR"
     gene            10542..>10961
                     /locus_tag="VFW59_12025"
     CDS             10542..>10961
                     /locus_tag="VFW59_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5364981.1"
                     /translation="MEERGNKTIMCSVFFLDIVGYSKKSVSGQISLKDRFNAYLSEAI
                     QGVPLADRIILDTGDGAAINFLGDVEDALKAALSLRENLLKEDVNSENPLLIRMGINL
                     GPVRLVRDINGQPNIVGDGINVAQRVMGFADSGQVLVS"
BASE COUNT         2062 a         2943 c         3638 g         2318 t
ORIGIN      
        1 cgggcgcgcc cgtcgattgc cgaggaggtc gcccatgtgc tggtgacgcg ccgggccgag
       61 ctggacagcg ccttgcagaa cctggacgag gagacgctgc accgggaaat cgaccatcgc
      121 cacaacgagg tgctggagac cgtcaagcga ttcttcggtt tgtagtatca tcgcgccgca
      181 tttttagcag ggatcaaata tgaaagtttc atttctggat tttgaaagct ccattgccga
      241 gctggaagac aagatcgaac agttgcgtta cgtgcaggac gactccgcgc tggatatcgc
      301 cgacgagatc agccgactgt cgaagaagag cgaggcgctg accaaggatg tctattccaa
      361 gctcacgccc tggcagatat cgcaggtctc gcgtcatcct cagcggcctt acacgctgga
      421 ttacatcgag catctgttca ccgacttcga ggagttgcac ggcgaccgtt cgtttgccga
      481 cgacaaggcc atcgtggctg gtttggcccg tttcaatggt cagagcgtga tggtgatcgg
      541 tcatcagaag gggcgcgata ccaaggaaaa gatcacgcgc aacttcggta tgccgcgtcc
      601 cgagggctat cgcaaggcga tgcgcctgat gaagctggcc gagaagttcg gtatcccgat
      661 catgaccttc atcgacaccc ccggcgccta tcccggcgtg ggcgcggaag agcgcggcca
      721 gtccgaggcg atcggccgca atctgtacga aatgtccgag ctgcgcgtgc ctatcatctg
      781 cacggtgatc ggtgagggcg gttccggcgg tgcattggcg atcgcggtgg gcgatgcgat
      841 gctgatgctg caatattcca cttattcggt gatttcgccg gaaggctgtg cctccatcct
      901 gtggaagagt gctgacaagg ctgccgatgc ggccgagacg ctcggtatca ccgcgactcg
      961 tctgaaggcg ctggggctgg tggacaagat tatcagcgag ccgctgggcg gcgcacaccg
     1021 cgactatgcc gcaaccatgc agagcgtgaa gaaggcgctg caggatgcac tcaaattggc
     1081 acagagcaag ccgacggcca acctgttgca ggatcgcttc aaccggttga tgagttatgg
     1141 caagttcaag gaagtcgaac tgtagaggca cggcgcgccg tgcccctacg acgagcatcg
     1201 tgtatttatc cggcggagaa cgtgtccgca cgttcatcct gaaaatgtcg gcaagggcgg
     1261 tcttttgagc cgccatgatc ttgtggatcg agtcgcggcg cgtctcgcgc cgttgctgcc
     1321 gccgcgcagc accatcctga tcggactgag cggcggggtg gattcggtcg tgctgcttca
     1381 cctgttgcat catcttgcgc cgcgcttcga ctggcggctc tctgccttgc atgtccatca
     1441 cggcatcagt ccgaatgccg atgcctgggc gcaattctgt accgaactct gcgctgccca
     1501 ggccgttccg ctgcacatcg aacgcgtgga tatcgcgccg ttgcgggaac acggcatcga
     1561 ggcggctgcg cgcaagctgc gtcatgccgc attcgccagc cagcgttgcg actttgtggc
     1621 gctggctcat catgccgacg atcaggccga gaccctgttg ctgcaattgt tgcgcggtgc
     1681 gggcgtgcgc ggcgtggcgt cgatgcctgt cctgagcaag gtcgaagggc cgttgctcgg
     1741 acaggctgcg gtggtgcgtc ccttgctcga tgttgcccgc gccacgctgc tcgactatgc
     1801 ctgccagcac cagctgcgct gggtggagga cgagagcaat gccgatgaga gctatccgcg
     1861 caacttcctg cgtcatcgtg cgctgccgtt gctggaggag cgcttccccg cctgtcgcga
     1921 gacgttggcg cgcagtgccc ggcacttcgc cgaggcgagc gagctgctcg atgatctggc
     1981 gcagatggat gccgagggtg cgatacaggg tgatgcgctg aatgtccctg tgttgcgttc
     2041 gctgacgcca ccccgcgcta aaaatttgct gcgccatttt ttgcaattgc aaggagcgcc
     2101 catgccgcag gctgctcagc tcgacgatat gctgcatcag ctatgtggcg cgcggcagga
     2161 tgcggcggta tgcgtggcct acggtggaca ttggcaggtg cgccgttatc aggaccagac
     2221 tttcgtgtcg cccgcattgg gcgaattgga tctcggtctg gtgatggcgt ggaatgggga
     2281 ggctgagctg agctggcccg cactcgagga gagtctgcgc ttcgacgaga cgcaaggggc
     2341 gggcatcagt ctggcgaagc tgcgccgcgc tcccgttaag ctatgtctgc gccacggtaa
     2401 agaacgattg cgtccgcatc cgcgtgcggc gaggcgcagc ctgaagcatc tgttgcaaga
     2461 acagggggtg ccgccctggc ggcgcgaacg cctgccgttg ctctattgcg gagatgagct
     2521 ggtgtgtgtg gtgggggtgg ccgtggcggc agagtatcag gctgggccgg gcgaagccgc
     2581 tattgtggtc gaactggcgg ggagcgccgg agaatagtat ttgtgggagc ggctaaagcc
     2641 gctcctgtaa cgctcccaca agacagcttc cgcctcgaat tggatggtca gggcaggaac
     2701 atgcgaaaca ccgcgccttc ggcattgctc agttcgatgc gcccatgccg ttctttcagt
     2761 ttatgcagct cggcgatctt tctggccaaa taaagcccca gcccggttcc ccggttgctg
     2821 acgaatgccg ggatgcctcc ctcgttcgtc agcatgccgg ccgggaagcc ggggccgtcg
     2881 tccgcgactt ccagaatcag catggcatcc tgttcgcggg cgctcagccg aacccgggtc
     2941 tttgcgctgc ggcaggcgtt atgtagggca ttggacagcg ccagccgtac cagtgcgtcg
     3001 tcaaagaagg cgaaggcggg ggcttgcgcc acgtcgatgt cgaaggtgag cgccgggaac
     3061 aattcgcggt attccgctgc cagtatcgcc aggaagtctg cggtatccac cgagtcctga
     3121 ttgacgctca gcaagtcgga ttcctggcga tgcaccagga gcacttcgga cagtcgccgc
     3181 gccgcgttca gcgcgatgac catttcctgc tgggcatcgt cgcgctcgcc caggcgcagc
     3241 gacagttcgg ccagttgatt cttgacatcg tggatgacgg ctgcgaacag atcgttcatg
     3301 gacgctctcc tgccgtttat ggcttcaatt gtttgagttg gctatcgatc tgggcgatct
     3361 tcggatagtc cggcgatttt gcgatgagcg cttcgcggta gcgcatgcac ttggagagtt
     3421 tctgtggatc gttgccgttc tgcaccagat ccagtgcgat caccagcgcc gcattcccca
     3481 cgatctgcag gttgttcggc aggcgattgg cggcctcgca cagcaatgcg accgcctcgt
     3541 cgagctgccc ggattcggcc ttgcgcacgc catcgttgtt gatgcgaatg atctcctgtg
     3601 tgctgtcctc gatcatggca tcggcttcgg tgacatcctt gcccgcagcc atgagcacgt
     3661 cgtgtacctt gcctctgatc gtctcgtctt ccggattgtt ctggatggcg tggcgcagca
     3721 gcccggtggc ttcctgttct ttcccaagcg tgaagcaggc gtccgcgatc gaggtgatgg
     3781 tctctgcgga taatgcgttg atgtctccgg tcatggcgcg ggcgagggcg gcttccgcca
     3841 gttcggcgtt cccggccgca tgttgcgcca cgctttcgct ggtcgccaga atgatctggc
     3901 tttgctcgtc gcgaaaatct ttgcgcgcct ccccgatgat gctcagagcc tcgttggctt
     3961 tgccctgatt ggccagtgca tgggacagca cggcgtaatc gtttacccgt ctaaccggcg
     4021 aatatttatg tttctgcaag gcctgccgca tcaccgcttc cgcgagttcc ggctggccgg
     4081 tgtcgaccgc cagcgtgctc agttcgcgta tgcggctcat ggtgcccggg gcgacttctc
     4141 gtgccttttg caatacctca agcgcggcct tcttgtcgcc ggatgaggac agcaggccgc
     4201 cgaggaagtc ataggccgcc atgaactggg gcgaatccgt caggatttcc tgcgcgagtt
     4261 gctgcgcctc gttcttctgg ttgagcaggg acagggcgcg cgccagtccc agtctggccc
     4321 agccgatcgg acgcaaggcg agcacttcgc gatacagctc ggcagcctct tgcgggcgat
     4381 tgtggtgcat cagtgccgcg cctttgatct tgcacagttc gatgaagtat ttgttcctgg
     4441 cgggaagcgc cttgtcgcat tctgcgatga cgtgcggcca gttttttgcg tcggtcgcct
     4501 gatccagatc cctgaagtac gcttgtcgct ccagcagttt caccaggcgc gcgttgaatt
     4561 gcccggccgt aaagggcttc agcaggtagt cgtcgggtgc gcactcggag gccgagacca
     4621 ctttttcata ggcctgttca gccgtgatca tcacgaaaat ggtgttgcgg ctgatcagat
     4681 cattggtgcg cagatgttcc agaaactgtt gcccgtcggt gccgctgccc agtgaatagt
     4741 cgcacaggat gacgtcgtag cgattgttct ccatgtgctg gagcgcttcc ctgatgttcg
     4801 ccaccacatg caacttggcg aaaccggaat tggataagga catgcgcagt tgcgaacgca
     4861 tgccttccat atcgtcgatg accagtactt gtttgtcggt gaattcgctg agggtggcca
     4921 atgcaatctt ccgggaattc aagatgttgc ttaccctacg catttgcagg agggcggtca
     4981 atagggcggg gctgagcgtt gagcctgtcg ggacgggcgg gcaattcaaa ttgacgctat
     5041 gcgcattgct gcacataatc ggttcgcata tttcacaccg aggtttttat gacggtcatc
     5101 aggcagcaag acttcatcga cagcatcgcg gatgcgctgc agttcatttc ctactaccac
     5161 ccgaccgatt ttattcgcgc gctggccgag gcttatcgcc tcgagcagtc gcctgcggcc
     5221 agggatgcga tggcgcagat actgacgaat tcgcgcatgt gcgcgcaggg ccaccggccg
     5281 atctgtcagg acaccggcat cgtcgtggca ttcgtgcggg tggggatgtc ggtgcgctgg
     5341 gaggcggata tggacatcgt cggcatggtc aacgccgggg tgcgccgggc ctatctcgat
     5401 tccgacaatg tgttgcgcgc ttccatcgtg tccgacccgg cggggcggcg caagaatacc
     5461 ggcgacaata cccccgccgt ggtgcatgtc gagctggtgc agggcgacaa ggttgaagtg
     5521 gagatcgcgg ctaagggcgg cggctcggag aacaaggcgg tgatggagat gctcaatccg
     5581 ggtgacgata tcgtcgaatg ggtattgcag cgcgtgccgg agatgggggc gggctggtgt
     5641 ccgccgggaa tgctgggcat cggcatcggc gggacggcgg agaaggcggt gctgctggcc
     5701 aaggaggcgc tgatgcagcc gatggacatg cacgaactga aggcgcgcgg accgcagaat
     5761 cgtttggagg agctgcgcat cgagctgtac gacaaggtca atgcgctggg catcggcgcg
     5821 caggggttgg gcgggctgac cacggtgatg gacgtgaaga tactcgacta tccgacccac
     5881 gcggcctcca agccgatcgc gatcattccc aattgtgccg cgacgcggca tgttcatttc
     5941 gaactggacg gctccggcgt cgcgacgttc gagccgccga agctcgagga ctatcccgat
     6001 gttcactggc atccggcgga ggatgcgcgg cgggtgaatc tggaaacggt cacgcgcgag
     6061 gaaatttccc attggcagcc gggcgagacg gtgttgctgt ccggcaaact gttgactggg
     6121 cgcgatgccg cgcataaacg catcgcggaa atgctggctc gcggcgaaaa gttgccatcc
     6181 ggcgtggatt ttaccggccg cttcatctat tatgtcgggc cggtcgatcc ggtgcgcgac
     6241 gaggtggtcg ggcccgccgg gccaactacc gcgacacgca tggatggcta cactgaaatg
     6301 atgctggcgc agaccggtct gatgggcatg gtgggcaagg ccgagcgcgg acgcgacgcg
     6361 atcgagtcga tcaaaaaaca cggtgccgtg tatctgatcg cgataggcgg tgcggcctat
     6421 ctggtttcga aggcgatacg aagtgcaaga gtcgttgcgt tccctgaatt ggggatggag
     6481 gcgatttacg aatttgaagt tgagaatatg ccggtgacgg tggcagtaga tgtgcgggga
     6541 cgttccgtgc atgacatcgg cccggcggaa tggcgcaagc gtatcggcat gatcccggta
     6601 catgcgacat aaaagaattg gcgggtctcc ccgcattgca gggtagcgaa tctggtcagg
     6661 tccggaagga agcagccata gctaattact gcaagtgccg ggggtaaggc tcgcctcctg
     6721 tttaaggcca ctaggagtgg tgatgaagat gaagctcggt tggctgataa gtgcgttgtt
     6781 gttcagcggt aatctgtatg cgttgggctc agtctctgtg gaatatggca gtgccttgca
     6841 gttcggtgat catctgggcg ccggggtgcg tttaggcgag catcagcagt ttgatctcgc
     6901 cttgcgcttt cagcatctat ccaatggtga tatcaagcag cccaatcatg gaatcaacct
     6961 caatcaagtt cgttttgctt ctcatttttg acgtggtgta acttgtcgac cacaactgta
     7021 ctcgcgcgca aatggcgtcc caggaacttc gcgcagctgg cggggcagga gcacgtcgtc
     7081 aaggcgctca gcaatgcgct gacgcagaat cgcttgcatc acgcctatct ttttaccgga
     7141 acgcgcgggg tgggcaagac caccatcgcg cgtatctttg ccaaatcgtt gaattgtgag
     7201 gcgggtatca ccgccacgcc ttgcggtgag tgcagcgcct gtcgcgagat cgatagcggg
     7261 cgttttgtcg atctgatcga actggatgcg gcgtcgaata ctcaggtcga cagcatgcgc
     7321 gagttgctgg agagtgcgct gtatgcgccg acttccgggc gcttcaaggt gtacatcatc
     7381 gacgaggtgc atatgctgtc caaatccgcc ttcaacgcga tgctgaagac gctggaagag
     7441 ccgcccggac atgtgaagtt tattctcgct accaccgatc cgcagaagat tccggtcacg
     7501 gtgttgtcgc gttgcctgca gttcaatctg aagcagttgc cgcccgcgct gatcgccagc
     7561 cgtctccagt atgtgctgga gcaggagcag ctcactttcg atgccggtgc gcttgctctt
     7621 gtcgctcgcg cggctcaggg cagcatgcgc gatgccttga gtctgatgga tcaggcgatc
     7681 gccttttcgt cgggcaaggt cgaagagtcg gtggtgcgca ccatgctggg ggcgatcgat
     7741 cacggttatc tgttcgaggt gctggcggcg ttgcgcgacc ggaatggtct ggcgttgatc
     7801 gagattgccg acaatatggc atcgcgcagc gtggctttcg atgcggcgtt gcaggaattg
     7861 gcgacactgc tgcatcgcat cgcgctggcg cagaccgtgc cgcaggcgat tgccgacgac
     7921 gagccggagc gggggcgttt gctggaactg gcgcaaacgt tgacgccgga gcaggtgcag
     7981 ctgtactacc agatcgccat tcacgggcgc gatgagatcg atcttgcgcc cgacgaatat
     8041 gccggtttct cgatgacgtt gctgcgcatg ttgtcgtttg cgccggctag aggggcggcg
     8101 atcgctacgg tgcccgtgtc tccggccgtg ttgcaagccc ctgtcgcgca acgccccgca
     8161 gtctcggagg tggccgttgc cgaagttgca cccgaaccgc gtagagtggc gccgtcattg
     8221 ggcggcgcgc tggattgggg ggtgttgctg gcacagttga atttgcaggg catggcgcaa
     8281 cagctggcca agcattctgt gctggagagc ctgtccgacg atctggtcac tttgtgtctg
     8341 tcgcaggaac agaagcatct gcaaaccaag atggccgtcg atcgtttgca ggcagcgctg
     8401 agcgagtatt ttgccaagcc ggtcaggttg aatatcgtgc tgggtaaagc cgaaacggca
     8461 acccccgcac tggttgaaca gcagaccaaa caactcaggc agcaagaggc gacagactcg
     8521 atcgaacatg atgttttcgt gcgtgatgca caggccgagt tggatgccag tgtgcttgaa
     8581 gaatcgatta aaccgataca gtgagaggga acaggaaatg atgaagggtg gcttgggtgg
     8641 tctgatgaag caggcgcagc agatgcagca gaacatgcag aaggcgcagg ccgagctggc
     8701 gaccgtggaa gtcgaggggc aggcggggtc cggcatggtg aaagtgttga tgacctgcgg
     8761 tcatgaagtg cgtcgcgtat cgctggatga cagcgtgctg ggagacgaca aggagatgct
     8821 ggaagatctg atcgtgctgg cgctcaacga tgcgatgaaa aaggcggagg cgaccacgca
     8881 acagcgcatg ggggctttca cctccggtat gggcttgccg cccggagtga agctgccgtt
     8941 ttgatgacat cttctcatct tgacgaattg atcgctgcgc tgcgctgtct gcccggtgtc
     9001 gggccgaagt cggcgcagcg catggcctat catctgctgc agcgggaccg cgccggcgcg
     9061 ctgcgtctgg cgcaatcgct ggagctggcc gccagcaaca ttctgcattg cagcaaatgc
     9121 aataattttt ccgagcacga ggtttgcgcc ctgtgtgcct cgtccaagcg cgatgccgag
     9181 gtgttgtgtg tggtcgagat gcctgccgat ctgttgatga tggagcaggc gcagtgctat
     9241 cgcggcatgt acttcgtgct gatgggacgt ttgtcgccgc tggacggtgt cggcgcgaaa
     9301 gagctgcatt tcgatcgctt gctcaagcgg gcaaaggaac aggtgcgcga agtgatactg
     9361 gcgaccaact tcaccgtgga aggtgatgcg accgcgcatt atttgacgaa tttgctgcga
     9421 acacagggta tcaaggtgtc gcgtatcgcg cggggactgc cggtcggtgg tgaattggag
     9481 caggtagata gcggcacact ggcgcaggcc gtactggaac gacgcgaggt gatggcatga
     9541 tcaagaagaa caggcgcaat ccggatgaac tccctgtcga gagcgaactg acagaggaga
     9601 aggatattgg cgagcggttg caaaaggtgc tcgcacaggc tggattcggc tcgcgccgcg
     9661 agatggaaga atggatcgcc aatgggcgcg tcagtgtgaa cggtgagccg gccacactgg
     9721 gtatgcgcgt ggtggcgggt gatctggtga agaccgagcg tcgcaccatt cgagtcggcg
     9781 agcgggatta tgctgcgcgc gtgctgctgt accacaagcc ggaaggcgag atcgtcagtc
     9841 gcgatgatcc ggaagggcgt accagtgtgt tcgacaagtt gcccaagctg cgcgggcaga
     9901 aatggatcgc catcgggcgg ttggatttca ataccagcgg attgctgatc tttaccacct
     9961 ccggcgatct ggctaaccgc ctgatgcacc cgcgtttcga agtggagcgc gagtatgcgg
    10021 tgcgtgtgca gggcactatg acggatgaac aaatgaccca gctgctcaag acgggggtcg
    10081 agctcgagga cggcttggtc aagtttgaaa agctggagga cgaaggcggc gaaggcttta
    10141 accactggta ccgtgtcatg ctcaaggaag ggcgcaatcg cgtggttcgg cgcagctttg
    10201 atgcgctggg tttgccggtc agtcgtttga tgcgggtgcg ctttggtata atcaatctgc
    10261 cgccgagact gaagcgcggt atgaccgccg agctggggga gggcgaggtg gcgcagattc
    10321 ttgagtgggt tggcattgct tccgatgagg tggaggccgt tgcgcctcgt cctgtgagga
    10381 atgcgcccgc aaagagcgat catcgtccag gtggcgcagc gcgaggcggt ccccggcgcg
    10441 gcaccgggaa aagtaaagct ggtgcgaagc gctgagtatc actagaatcg gcgctacttt
    10501 tgattgagac acgaaacgtt agctgagcaa tggaagcctg aatggaagaa cgaggcaaca
    10561 agaccatcat gtgcagtgtt tttttcttgg atatcgtggg atattccaag aaatcagtgt
    10621 ctggacagat atccctgaaa gataggttca atgcctatct ttccgaggca attcaggggg
    10681 tgcccttggc ggatcgaatc atcctcgata caggggatgg tgcggctatc aatttcctcg
    10741 gcgatgtgga ggatgcgctc aaggcggcgc tgagtctgcg cgagaatctg ctcaaagaag
    10801 atgttaactc ggagaacccc ctgctgatcc gcatgggcat caatcttggg cccgtgcgac
    10861 tggtcaggga tatcaacggg cagcccaata tcgtgggtga tggcatcaat gtcgcacagc
    10921 gagtgatggg cttcgccgat tccgggcagg tgctggtgtc g
//
LOCUS       DASUIV010000339         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1020097, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000339 DASUIV010000000
VERSION     DASUIV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020097"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFW59_12030"
     CDS             complement(<1..440)
                     /locus_tag="VFW59_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5364982.1"
                     /translation="MVSLQPPLCDFGWKAPDFDLPGVDGKRYNLASARGKNGLLVMFI
                     CNHCPYVKSIRDRIVRDVRELQQHGINSIAIMSNDPADYAEDSFENMKKVAQEFSYPF
                     PYVIDETQQVAKDYGAVCTPDFFGFNANMELQYRGRLDASRKEAA"
     gene            515..1246
                     /locus_tag="VFW59_12035"
     CDS             515..1246
                     /locus_tag="VFW59_12035"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX5364983.1"
                     /translation="MPRFYCPSSLPPSGSFDLPPDAAHHASRVLRLREGDTVEIFDGN
                     GNERRGVIADLGGKRVVIGELTVTNSERESPLPTLLAQSLLSSEKMDWVIQKATELGA
                     NEIQPLATERSVARLSAERTEKRREHWQQVAISACEQCGRNVLPIIHAPLDIMVWLKQ
                     MQASTDSKFILLPQGATALQDQPRPQGRAVLLIGAEGGFAPAESETAVHCGFAPIRMG
                     ARVLRTETAAVAGLAALQTLWGDFN"
     gene            1265..1450
                     /locus_tag="VFW59_12040"
     CDS             1265..1450
                     /locus_tag="VFW59_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEX5364984.1"
                     /translation="MPYVNIRIAGTLSREQKARIAAEITDTLERIALKPKSYTYIAFD
                     ELPDENWAIAGQLLDEQ"
     gene            1489..>2702
                     /gene="argA"
                     /locus_tag="VFW59_12045"
     CDS             1489..>2702
                     /gene="argA"
                     /locus_tag="VFW59_12045"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX5364985.1"
                     /translation="MPATTAPADFVAWFRSVAPYINAFRGKTFVIAFGGEVVADGKFV
                     ELTHDFNLLAALGIRLVLVHGARPQIEQHLAKNQLSDTYHHGIRMTDAQTMQCVKEAV
                     GRVRLEIEALLSMGLANSPMANADIRVAGGNFITAQPIGVINGVDLQHTGTVRKVDVA
                     ALNDRMEFGEVVLLSPLGYSPTGEVFNLTLEDVATATAIAMDADKLVFLMDTDGVQGA
                     KGELLKELTVAQAQKVLGGKRKLSDDVSLFLPCAIRACEAGVARTHLISRHTDGSVLQ
                     ELFSDEGIGSMVVESALNTLREASIDDIGGIIELLRPLEESGILVRRSRELLEREIDR
                     FVVLEHDHRIIGCAALYPFPDEAAAELACLAVDAQCRDRGYGEAVLNHMISVAKSQGL
                     KKLFVLTTRTAH"
BASE COUNT          550 a          856 c          826 g          470 t
ORIGIN      
        1 gccgcttcct tgcgcgaggc atccaggcgg ccgcgatatt gcaactccat gttggcgttg
       61 aaaccgaaga aatcgggagt gcacaccgcg ccgtagtcct tcgcgacctg ctgagtctcg
      121 tcgatcacat aggggaaggg ataggaaaat tcctgtgcga ccttcttcat gttctcgaac
      181 gagtcttccg cgtaatcggc aggatcgttg gacatgatgg cgatactgtt gatgccatgc
      241 tgctgcagtt cgcgcacatc gcgcacgatg cggtcgcgga tggatttcac ataggggcaa
      301 tgattgcaga tgaacatcac cagcaggccg ttcttgccgc gagcgctggc gaggttgtag
      361 cgcttgccat ccacgccggg cagatcgaaa tcaggagcct tccagccgaa atcgcacagg
      421 gggggttgca gactgaccat tcgaaattcc tcacaacaaa tttgaaccga tatattatca
      481 cgctggactg ttctgattcc ccgctcacat accgatgcca cgcttctact gcccctcgtc
      541 actgccgccc agcggctcct tcgatctgcc gcccgatgcg gcccaccatg cctcgcgcgt
      601 actgcgcctg cgcgagggcg atactgtcga aatcttcgat ggcaacggca acgaacggcg
      661 cggcgtgatc gccgacctcg gcggcaagcg cgtggtaatc ggcgagctga cggtgacaaa
      721 cagcgagcgc gaatcgccgt tgccgacgct gctggctcaa tcgctgctga gcagcgaaaa
      781 gatggactgg gtgatccaga aggccaccga gctgggtgct aacgagatcc agccgctcgc
      841 caccgaacgc agcgtggcga ggctgtctgc cgaacgcacc gagaaacggc gggaacactg
      901 gcaacaggtc gccatctcgg catgcgaaca atgcggtcgc aatgtgctgc cgatcatcca
      961 tgccccgctg gacatcatgg tctggctgaa acagatgcaa gcatcgactg acagcaaatt
     1021 catcctgctg ccgcagggcg cgaccgcgct gcaggatcag cccagaccgc aagggcgtgc
     1081 ggtgctgctg atcggcgcgg agggcggctt cgccccagcc gagagcgaga ctgcagtgca
     1141 ctgcggcttc gcaccgatcc gcatgggcgc acgcgtgctg cgcaccgaga ccgccgccgt
     1201 cgccggactg gccgcactgc aaacgctgtg gggcgatttt aattaacagg aaacaggagg
     1261 aatcatgccc tacgtcaaca tccgcatcgc cggaacactc agccgcgaac agaaggcccg
     1321 gatcgccgcc gaaatcaccg acacgctgga gcgcatcgcg ctcaagccca agtcttacac
     1381 ctacatcgcc ttcgacgaac tgcccgacga gaactgggcc atcgcggggc agttgctgga
     1441 tgaacaatag cgcggcatga tgcacgcact caaacaggga gtccattaat gcccgccacc
     1501 actgcacccg ccgatttcgt agcctggttc cgctccgtcg caccgtatat caatgcgttt
     1561 cgcggcaaga cctttgtcat cgcgttcggc ggcgaggtgg tggcggacgg aaaattcgtc
     1621 gaactgaccc acgacttcaa cctgctcgcc gcactcggca tccggctggt gctggtgcat
     1681 ggtgcgcgac cgcagatcga acagcatctg gcgaagaacc agctcagcga tacctaccac
     1741 cacggcatcc gcatgaccga tgcccagacc atgcagtgcg tcaaagaagc cgtcggccgc
     1801 gtgcggctgg agatcgaggc gctgctgtcg atggggctgg cgaattcgcc gatggccaac
     1861 gccgacatcc gtgtggcggg cggcaacttc atcaccgctc agcctatcgg cgtgatcaac
     1921 ggcgtggatc tgcagcacac cggcaccgta cgcaaggtgg atgtcgccgc actgaacgac
     1981 cgcatggaat tcggcgaggt cgtgctgctc tcaccgctcg gctattcgcc caccggcgag
     2041 gtgttcaatc tgacactgga agatgtcgcc accgccaccg ccatcgcgat ggatgccgac
     2101 aagctggtgt tcctgatgga taccgacggt gttcaaggcg ccaagggcga gctgctcaag
     2161 gagctcaccg tcgcccaggc gcagaaagtg ctgggaggca aacgcaagct gtcggacgat
     2221 gtgagcctgt tcctgccctg cgccatccgc gcctgcgaag ccggagtggc gcgcactcat
     2281 ctgatcagcc ggcacaccga cggctcggtg ctgcaggaac tgttcagcga cgagggcatc
     2341 ggcagcatgg tggtggagag cgcgctcaac accctgcgcg aggcttccat cgacgatatc
     2401 ggcggcatca tcgaactgct gcgcccgctg gaggagagcg gcattctggt acggcgttcg
     2461 cgcgagttac tggaacgcga gatcgaccgc ttcgtggtgc tggagcacga ccaccgcatc
     2521 atcggctgcg ccgcgcttta ccccttcccg gacgaggccg ccgctgaact cgcctgtctg
     2581 gcggtggatg cgcagtgccg cgatcgcggc tacggcgagg ccgtgctcaa ccacatgatc
     2641 tcggtcgcca aatcacaggg tctgaagaaa ctgttcgtgc tgacgacgcg caccgcacac
     2701 tg
//
LOCUS       DASUIV010000340         6227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1113410, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000340 DASUIV010000000
VERSION     DASUIV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113410"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /gene="hemH"
                     /locus_tag="VFW59_12050"
     CDS             <1..936
                     /gene="hemH"
                     /locus_tag="VFW59_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5364986.1"
                     /translation="AIWWLILNGIILNTRPKKSAAKYASIWLKEGSPLRVYTEKQAVL
                     LQGYLSQRTKAAFAVDFAMRYGNPSIPDALRRLKEQNCQRILIVPMYPQYAASTTATA
                     CDVVFDALQQMRNMPALRTIKHFHDDAGYIRALAASINDYWMKHGRPEKLVMSFHGLP
                     RYTLEKGDPYHCECQKTGRLLAQELGLKPEQYAISFQSRFGRAEWLQPYTTATLKALG
                     KQQTARVDIVCPGFVADCLETLEEIAQEGKEDFQHAGGGEYHYIPCLNDRDDWMHALT
                     DLVMDNLQGWLSETDANELEQSRLRALAMGAKSQA"
     gene            933..3275
                     /locus_tag="VFW59_12055"
     CDS             933..3275
                     /locus_tag="VFW59_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5364987.1"
                     /translation="MTEQIISDRFKILSELGRGGQGSVYLAHDKQLDRKVAIKTLRKF
                     SHQTDQLTREALIVSKLQHPNIITLHDAGDHNGTPYLIYAYIEGKTLAQVLKGEKTLP
                     FARAAEIACDALEGLSSAHSQGVAHLDIKPANIMIAKSGVAMVMDFGLATTSNTDEQT
                     ATGMLNGTPRYVAPEAISGKPGTALSDIYAIGAVLYEMVTGHYAVTGESLYEVLNRAA
                     NEQVTAPTAHNQAIDKKLEEIILKALSKNPADRYPSAVAMLKELREYLNESRSATMEF
                     LLQRMRSKQDFPAMSGVISEINKIVSSEEESSSRLASVILQDLALTNKLLRLVNTASF
                     SRFGGSINTISKAVVILGFGTVRNIATSLILLEFMQNRSQAAQLKEEIVKAVLAGELA
                     AQLSSRQDIRDVEEILVCAMFHNLGKMLAIFYFFEESQEIARLTREGENEDSAAAKIL
                     GIPYSELGQRVARNWNFPPRLLAGMRKLSGDNISPPKDELDRLSTTINLAYDLCDMSA
                     TSRAQEKSQQLTELSKRYEDAVKLSERELSNALKTGLNEMGARCGILGISPTSSPLLA
                     RAREWSGHSPTTPKAKDNGMGGMTDLGQSVAQKYDENETLTLSLNPDSILGAGIQEVT
                     NTMMGEFKLNEVLQMVLETMHRGMGFHRTLFLIRNNKLGAMNARFGFGPRIGEIIPNF
                     HFPLAFVSDVFHLSIAKGLDISIEDVRSLKIADKIPHWYRHTVNAPCFILLPLMLQGK
                     AIGLIYADMQEANHLDISHQQLSLLRTLRNQAVLAIKEKV"
     gene            3418..5775
                     /locus_tag="VFW59_12060"
     CDS             3418..5775
                     /locus_tag="VFW59_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5364988.1"
                     /translation="MTAQTIGRFEIIRELGKGGQGMVYLAKDTQLDRGVAIKTLRNLA
                     HQSEQLAHEARIVSKLQHPNIIPLYDAGEHQGAPYLVYAYIDGQTLDQQLKQEEDLSL
                     VRAAEIACDILEGLAYAHAQNISHFDIKPANIMIASNGTAMVMDFGLAKLANRQEAGN
                     GQLSGTPRYMAPELIAGKPADFTADIYAVGVMLYEMVTGEFAVGGSNVYEVLNRAANE
                     RITAPSTHNAQIDERLEGIILKAVAKSPDERYPSCESMLEALRGYLGELRENGVHEPQ
                     SHSTLEFLLRRMRSKQDFPALSNIISEINKIVGADSDGANKLASTILQDFALTNKLLK
                     QVNAASYGQFGGSINTVSKAVVILGFETVRNIAMTLILLEFLQNKAQAADLKDEVVLA
                     ILSGLVAAQLLDEHDFRDAEEIMVSAMFHNLGRMLGIFYFFDESQEIARRVEQGENED
                     SAAIHVLGMPYPELGLGVARSWNFPPRLIAGMRKLSRDPVPAPKDELDRLTVTVNLAH
                     ELCKTAANTDIQDKIRSLKALVQRYEGAGKLSERKLSGAMESGLNELSLRASALGVGV
                     HNSAMLKRVQRWNGQAPATELAKRDQALNGITDLDQTVMQGDEGDSPKHNPEAVLGAG
                     IQDVTTSLVGDFKLNDVLQMVLETMYRGMGFSRALILIRDNQKDAMVARFGFGTDVQK
                     IIPRFHFPLHFVPDVFHLSIQKGLDIAIEDVHAPNIADKIPLWYKDNIDAPCFMLLPI
                     MVKEKVIGLFYADMIEANALKLSQQQLSLLRTLRNQAVLAIKQKT"
     gene            5889..>6227
                     /locus_tag="VFW59_12065"
     CDS             5889..>6227
                     /locus_tag="VFW59_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5364989.1"
                     /translation="MNLVKWDPFKELEDVSSRLNRLFGRPALPVESDREMLAVADWMP
                     SVDISETDIAYLIKAEIPGVKKEDVKVTIEDGMLTIQGERKQEKEEKDKKFHRIERSY
                     GCFMRSFRVPD"
BASE COUNT         1420 a         1977 c         1780 g         1050 t
ORIGIN      
        1 gcgatctggt ggctgatcct caacgggatc atcctgaaca cccgcccgaa aaaatccgcc
       61 gcgaaatacg ccagcatctg gcttaaagag ggctccccgc tgcgcgtcta caccgagaaa
      121 caggccgtgc tgttgcaggg ctatctgagc cagcgcacca aggcggcgtt cgcggtggac
      181 ttcgcgatgc gttacggcaa cccttcgata cccgacgcgc tgcgccgcct caaggaacag
      241 aactgccagc gcatcctgat cgtgccgatg tatccgcaat acgccgccag caccaccgcg
      301 acggcgtgcg atgtggtgtt cgacgcgctg caacagatgc gcaacatgcc cgcgctgcgc
      361 accatcaagc acttccacga cgatgcgggc tacatcaggg cgctggcggc cagcatcaac
      421 gactactgga tgaaacacgg ccgcccggaa aagctggtga tgagtttcca cggcctgccg
      481 cgctacacac tggagaaggg cgacccctat cactgcgagt gccagaagac cggacgcctg
      541 ctggcgcagg agctcggcct gaagcccgag cagtacgcga tcagcttcca gtcgcgcttc
      601 ggccgtgcgg agtggctgca accctacacc accgccacgc tcaaggcgct gggcaaacag
      661 cagaccgcgc gcgtggacat cgtctgcccc ggcttcgtcg ccgattgcct ggagacgctg
      721 gaggagatcg cgcaggaagg taaagaggac ttccagcatg cgggcggcgg cgagtatcac
      781 tacatcccct gcctcaacga tcgcgacgac tggatgcatg cgctgaccga tctggtgatg
      841 gataacctgc agggctggct gagcgaaacc gatgcgaacg agctcgaaca gagccgcttg
      901 cgcgcgctgg cgatgggggc gaagagccag gcatgaccga gcaaatcatc agcgaccgtt
      961 tcaagatcct cagcgaactg gggcgcggcg gacagggttc ggtctatctc gcccacgaca
     1021 agcaactgga tcgcaaggtc gccatcaaga cgctgcgcaa gttcagccat cagaccgatc
     1081 agctgacccg cgaggcgctg atcgtcagca agctgcagca tcccaacatc atcacgctgc
     1141 acgatgcggg cgaccataac ggcacgccct atctgatcta tgcctacatc gaaggcaaga
     1201 ccctcgcgca ggtgctcaag ggagagaaga cgttgccctt cgcacgtgcg gctgagatcg
     1261 cctgcgacgc gctggaagga ctgtccagcg cgcactcgca gggcgtcgcg cacctggaca
     1321 tcaaacccgc caacatcatg atcgccaaga gcggcgtggc gatggtgatg gatttcgggc
     1381 tggcgacgac cagcaatacc gacgagcaga ccgccaccgg catgctcaac ggcacgccgc
     1441 gctatgtcgc gcccgaggcc atctccggca agcctggcac ggcgctgtcg gacatctacg
     1501 ccatcggcgc ggtgctctac gagatggtca ccgggcatta cgccgtcacc ggcgagagcc
     1561 tctacgaggt gctgaaccgt gcggccaacg agcaggtgac cgcaccgacc gcgcacaatc
     1621 aggccatcga taaaaaactt gaggagatca tcctcaaggc gctgtcgaag aaccccgccg
     1681 accgctatcc aagcgccgtc gcgatgctca aggagttgcg cgaatatctg aacgaatcgc
     1741 gcagcgcgac catggaattc ctgctgcagc gcatgcgcag caaacaggac ttcccggcca
     1801 tgtccggcgt catctccgag atcaacaaga tcgtctcttc ggaagaggaa agcagcagcc
     1861 gactggccag cgtcatcctg caggatctgg cgctgaccaa caagctgctg cggctggtga
     1921 acaccgcctc cttcagccgg ttcggcggct ccatcaacac catctccaag gccgtggtga
     1981 tcctcggttt cggaacggtg cgcaacatcg cgacctcgct gatcctgctc gagttcatgc
     2041 agaaccgctc gcaggccgcg cagctcaagg aggagatcgt caaggccgtg ctcgccggcg
     2101 aactggccgc gcaactctcg agcagacagg atatccgcga tgtcgaagag atactggtct
     2161 gcgcgatgtt ccacaatctg ggcaagatgc tggcgatctt ctacttcttc gaggaaagcc
     2221 aggagatcgc acgcctgacc cgcgagggcg agaacgaaga ttccgccgcc gcaaaaatac
     2281 tgggcatccc ctattcggaa ctgggccaga gagtggcgcg caactggaac ttcccaccgc
     2341 gcctgcttgc gggtatgcgc aaactatcag gggacaatat ctctcctccc aaggacgagc
     2401 tcgaccgcct gtccaccacg atcaatctgg cctatgacct gtgcgacatg tcggccacca
     2461 gccgcgcaca ggaaaaatcc cagcaactga ccgaactttc caaacgctac gaggatgcgg
     2521 tcaaactctc cgaacgcgaa ctgtccaacg cgctcaagac cgggctgaac gagatgggcg
     2581 cgcgctgcgg catactcggc atcagcccca ccagcagccc cttgctcgcc cgcgcacgcg
     2641 aatggagcgg ccactcgccg accacgccga aagcaaagga taacggcatg ggcggcatga
     2701 ccgatctggg tcaatccgtc gcgcaaaaat acgatgaaaa cgaaaccctc accctctcgc
     2761 tgaacccgga ttcgatactc ggcgccggta ttcaggaggt gaccaacacc atgatggggg
     2821 agttcaagct gaacgaggtg ctgcagatgg tgctcgagac catgcaccga ggcatgggat
     2881 tccatcgcac cctgttcctg atccgcaaca acaagctggg ggccatgaac gcccgtttcg
     2941 gattcggccc gcgcatcggc gagatcattc cgaactttca cttcccgctt gcctttgtgt
     3001 ccgacgtgtt ccacctgtcc atcgcgaaag gcctcgacat ctccattgaa gatgtgcggt
     3061 cgctcaagat tgccgacaag attccgcact ggtatcgcca caccgtcaat gcgccctgct
     3121 tcatcctgct gccgctgatg ttgcagggca aggcgatcgg cctgatctac gccgacatgc
     3181 aggaggccaa ccatctcgac atctcgcacc agcagctttc gctgctgcgc accctgcgca
     3241 accaagcggt gctggcgatc aaggaaaagg tctgaccccc tgaaaagcca cgcgccccgc
     3301 cactcgatgg gcgcagggaa gcggcggcac atggtgtaga atgccgggca atatttccat
     3361 cgcgagactg tttgcatcca tgcctctttg ccgccctttt ttcgcgcccg atctccgatg
     3421 acggcacaga ctatcggtcg tttcgaaatc atccgcgagc tcggcaaggg cggtcaaggc
     3481 atggtgtatc tggcaaaaga cacccagctc gaccgaggcg tcgccatcaa gaccctgcgc
     3541 aatctggcgc accagtccga gcaacttgca cacgaagcgc gcatcgtcag caagctgcaa
     3601 cacccgaaca tcattccgct gtacgacgcg ggcgaacatc agggggcacc ctatctggtc
     3661 tatgcctaca tcgatggcca aacgctggat cagcaactga aacaggaaga agacctgtct
     3721 ctggtgaggg cggcagagat cgcctgcgac atactggaag ggctggccta cgcccatgcc
     3781 cagaacattt cccacttcga tatcaaaccg gccaacatca tgatcgccag caatggcacg
     3841 gccatggtga tggatttcgg tctggcgaaa ctggccaatc ggcaggaggc gggtaacggc
     3901 cagctgagtg gcacaccgcg ctatatggcg ccggaactca tcgcgggcaa accggccgat
     3961 ttcaccgccg acatctatgc cgtcggcgtg atgctctacg aaatggtgac cggggagttt
     4021 gcggtcggcg gcagcaacgt ctacgaagta ttgaatcgcg ccgcaaacga acgcatcacc
     4081 gcgccctcga cccacaacgc gcagatcgac gaacggctcg aaggcatcat cctcaaggcc
     4141 gtagccaaga gccccgatga gcgctatccg agctgcgagt ccatgcttga ggccttgcgc
     4201 ggctatctgg gcgaattgcg cgagaacggt gtgcatgaac cgcagagtca cagcacgctg
     4261 gaatttctgc tgcgccgcat gcgcagcaaa caggacttcc cggcgctgtc caacatcatt
     4321 tccgagatca acaagatcgt gggagcggat tcggacggcg ccaacaaact ggctagcacc
     4381 atcctgcagg attttgcgct gaccaacaaa ttgctcaagc aggtcaacgc cgcttcctac
     4441 gggcagttcg gcggcagcat caacaccgtg tccaaggccg tggtcatcct cggtttcgag
     4501 acggtgcgca acatcgcgat gacgctgatc ctgctggagt tcctgcaaaa caaggcacag
     4561 gcggcagatc tcaaggacga ggtcgtgctg gcgatcctgt cgggcctcgt cgccgcacaa
     4621 ctgctggacg aacacgactt ccgcgatgcc gaagagatca tggtcagcgc gatgttccac
     4681 aatctgggcc gcatgctggg catcttctac ttcttcgatg agagccagga aatcgcccgg
     4741 cgcgtcgaac agggcgaaaa cgaagacagc gcagccatcc atgttctggg catgccctac
     4801 cccgaacttg gcctgggcgt ggcgcgcagc tggaacttcc cgcccaggct gatcgccggc
     4861 atgcgcaagc tctccagaga ccccgttccc gccccgaaag acgaactgga tcgcctgacc
     4921 gtcaccgtca atctggccca tgagctctgc aagacggccg caaacacgga tattcaggac
     4981 aagattcgct cactgaaagc gttggtccag cgttatgagg gcgcgggcaa gctgtccgaa
     5041 cgcaagctct ccggcgccat ggagtccgga ctgaacgagc tgtcgttgcg cgccagcgcg
     5101 ctcggcgtgg gcgtccacaa cagcgcgatg ctgaaacgcg tacaacgctg gaacggccag
     5161 gctccggcga ccgaactggc caaacgcgac caggccctga atggcatcac cgatctcgat
     5221 cagacggtca tgcaaggcga cgaaggcgac agcccgaaac acaatccgga agccgttctc
     5281 ggcgcgggca tacaggatgt caccacctcg ctggtgggcg acttcaaact caacgatgtg
     5341 ctgcaaatgg tactggagac catgtatcgc ggcatgggct tcagccgtgc gctgatattg
     5401 atccgcgaca accagaagga cgccatggtg gcacgcttcg gcttcggcac cgacgtgcag
     5461 aagatcatcc cgcgttttca ttttccgctg catttcgtgc cggacgtatt ccatctgtct
     5521 atccagaaag gtctggacat cgccatcgag gacgtccacg cgcccaatat cgccgacaag
     5581 attccgctct ggtacaagga caatatcgac gccccctgct tcatgctctt gcccatcatg
     5641 gtcaaagaga aggtgatcgg gctgttctat gccgatatga tcgaagcgaa cgcactcaaa
     5701 ctctcgcagc agcaactttc cttgctgcgc acgctgcgca atcaggccgt gctggccatc
     5761 aaacagaaga cctgaacgca agtcgctctg cattttcctg atagcaaaat catccttatc
     5821 cgatagcgcc gcgacaaagc ggcatgacac catcggctct cctaccaaca acgcaaagga
     5881 gagaagccat gaatctggtc aaatgggatc ccttcaaaga actggaagat gtctccagcc
     5941 gcctgaaccg gctgttcgga cgcccggcgc tgccggtcga atccgatcgc gagatgctag
     6001 cggtggcgga ctggatgccc tcggtcgata tcagcgaaac ggatatcgcc tatctgatca
     6061 aagccgagat tcccggcgtg aagaaagaag acgtcaaggt caccatcgaa gacggcatgc
     6121 tcacgataca gggcgagcgc aaacaagaga aagaagagaa ggacaagaaa ttccaccgca
     6181 tcgaacgctc ctacggctgc ttcatgcgca gcttccgcgt gcccgac
//
LOCUS       DASUIV010000341         6522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1113817, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000341 DASUIV010000000
VERSION     DASUIV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113817"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..673
                     /gene="arfB"
                     /locus_tag="VFW59_12070"
     CDS             260..673
                     /gene="arfB"
                     /locus_tag="VFW59_12070"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX5364990.1"
                     /translation="MLYISHHVSIADQEIEMTAVRAQGAGGQNVTKVSSAVHLRFDIA
                     ASSLPPEFKQRLRQLNDQRITKDGVIIIKAQSFRSQDKNRGEALRRLKELVQSIAVLP
                     AERRATKPTRSSQRKRVDSKTLRGRVKAMRGKVME"
     gene            930..1112
                     /locus_tag="VFW59_12075"
     CDS             930..1112
                     /locus_tag="VFW59_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364991.1"
                     /translation="MHVLLSMLPILLTGVIVLMAGIIISRKPGQSRSCAFIESLVWGG
                     GFFIALSIEILTFANG"
     gene            complement(1181..3427)
                     /locus_tag="VFW59_12080"
     CDS             complement(1181..3427)
                     /locus_tag="VFW59_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HEX5364992.1"
                     /translation="MKFRFPIVIIDEDFRSENASGLGIRALADAIEKEGMEILGVTSY
                     GDLTSFAQQQSRASAFLLSIDDEEFGAGSQEETEVALKTLRAFVAEIRFKNADIPIYL
                     YGETRTSRHIPNDILRELHGFIHMFEDTPEFVARHIIREAKSYLDSLAPPFFRALLDY
                     AQDGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPV
                     AASERNAARIFNADHLFFVTNGTSTSNKIVWHSTVAPDDIVVVDRNCHKSILHSIMMT
                     GAVPVFLTPTRNHYGIIGPIPKSEFEPASIQRKIDANPFIKDKTKKPRILTITQSTYD
                     GVVYNVEMLKEMLDGRIDTLHFDEAWLPHAAFHDFYKDMHAIGKDRPRANESMIFATQ
                     STHKLLAGLSQASQILVREPQSRQLNRHIFNEAYLMHTSTSPQYAIIASCDVAAAMME
                     PPGGTALVEESIAEALDFRRAMRKVDEEFGQDWWFKVWGPDVIAEEGMGSREDWVLKP
                     SDKWHGFGDLAEGFNMLDPIKATIITPGLDVDGDFADSGIPASMVTKYLAEHGVIVEK
                     CGLYSFFIMFTIGITKGRWNTLLTALQQFKDDYDKNAPIWRILPEFAAKHPHYERIGL
                     RDLCQQIHDAYKQHNVARMTTEMYLSDMQPAMKPSEAFAKMAHDEIDRVEIDDLEGRI
                     TAVLLTPYPPGIPLLIPGEMFNKTIVDYLKFARDFSQTLPGFETDIHGLVAEKVDGVK
                     RYFVDCVR"
     gene            complement(3786..4007)
                     /locus_tag="VFW59_12085"
     CDS             complement(3786..4007)
                     /locus_tag="VFW59_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364993.1"
                     /translation="MSNDTQIVWRSPKGEVIACIEKNKVMQENLVEIRQVCRDALEDA
                     VLMGCDEQQFRAVLAELVVSLESSFPPQD"
     gene            complement(4023..4589)
                     /gene="dcd"
                     /locus_tag="VFW59_12090"
     CDS             complement(4023..4589)
                     /gene="dcd"
                     /locus_tag="VFW59_12090"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX5364994.1"
                     /translation="MSIKSDKWIRRMAEQHGMIEPFEPGQVKEQNGQKIVSYGTSSYG
                     YDIRCSREFKLFTNINSTIVDPKNFDPNSFVNVEADYCVIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGVAQVLFF
                     ESDEVCETSYKDRGGKYQGQHGVTLPKM"
     gene            complement(4643..4867)
                     /locus_tag="VFW59_12095"
     CDS             complement(4643..4867)
                     /locus_tag="VFW59_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5364995.1"
                     /translation="MSYFIYRITEQPIRMLSKLEQHDTYREAASRVKQLRAELPATSP
                     HVVKMIFAETEFHAEDLLNQVREASPEADD"
     gene            complement(5029..5493)
                     /locus_tag="VFW59_12100"
     CDS             complement(5029..5493)
                     /locus_tag="VFW59_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5364996.1"
                     /translation="MNLFDAAKNGDLVTVKALIESGADVNATDERGWSALACAVYNAE
                     QKRGFAEVTLALIAAGADIEATIGFGVRPLMLAAGYGEAEVVATLLNAGADPLARNEG
                     GYTALMMAKQKHYVDVINLLHEAEYYAGAGEGSCESRNPPGSSVVTFMKRPA"
     gene            complement(5490..>6522)
                     /gene="cysG"
                     /locus_tag="VFW59_12105"
     CDS             complement(5490..>6522)
                     /gene="cysG"
                     /locus_tag="VFW59_12105"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041353255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEX5364997.1"
                     /translation="LMVAFSSGGASPVLTRMMRGKLETVIPQNYSRLAAFAERFRELV
                     KTRVTNPAKRRIFWENAFEGVVAEKVLTGDESSAEAMLLKMLESEDNIQRGEVYLVGA
                     GPGDPDLLTFRALRLMQKADVVVYDNLVSKPIVEMTRRDAERIFVGKQRADHTLRQEE
                     INELLVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLAAENIPFQVVPGITAASGVASY
                     AGIPLTHRDYAQSCMFVTGHLKDGTMNLDWDALARPKQTVVVYMGLHGLDILCGKLIE
                     HGMPDTTPIAIVQQGTTQNQRVFTGTLATLPGIAEREKPQAPTLIIVGGVVALREKLA
                     WFHPQGQEK"
BASE COUNT         1320 a         1987 c         1886 g         1329 t
ORIGIN      
        1 gcggtacgcc gtgctcctgc agcgacaccc gcccgcaagg ctactccggc caaagcgcca
       61 gccaaggcac ctgcaagggc cgccaaggct cctgcgaagg cctcggcgaa gcctgcggac
      121 gacgaggagt gggaagagtt ctaagctccg gatagcgtca ttgataaaaa ggcagcgcag
      181 atgcgctgcc tttttttgtg cctgctgaac accacacatg cgccataatg cgcgcatccc
      241 tccctatcgc ggaactcgca tgctgtatat ctctcatcat gtttcgatcg ccgatcagga
      301 gatcgagatg acggcagtgc gcgcgcaagg cgcgggcggt cagaacgtca ccaaggtttc
      361 cagcgcagta catctgcgct tcgatatcgc cgcctcctcg ctcccgccgg aattcaaaca
      421 acgcctgcga caactgaacg accagcgcat caccaaagat ggcgtgatca tcatcaaggc
      481 acagtcgttc cgcagccagg acaagaatcg cggtgaggca ttacggcgtt tgaaagaact
      541 ggtgcaaagc atcgccgtgc tgcccgccga acgccgcgcc accaaaccga cacgcagctc
      601 gcaaagaaag cgcgtcgaca gcaagaccct gcgcggcagg gtcaaggcga tgcgtggcaa
      661 agtaatggaa tgatcccggc agcaatacca gcacccagca acatggcgat caacttgatc
      721 gcagtccgtt cggatcgggc gaacatgatt ctgcaggatg agatctcgtc tttttcctag
      781 tacgcgcttc cacaattccc atccgagaaa agtcttttca ctatccttca gccgaaacca
      841 actggagcag aatccgtcct gccacgacaa ccgtgccgca ccctgccgca acggttaacg
      901 aagacgcacc catcatcaag gaggttcaga tgcatgttct actatccatg ctgccgatac
      961 tgctgaccgg cgtgatcgtg ttaatggcag gcataatcat cagcaggaaa ccgggccaaa
     1021 gccggagctg cgcgttcatc gagagcctgg tttggggtgg cggctttttc atcgcgctca
     1081 gcatcgaaat actgaccttt gccaacggct agaagcatgg ctcagcaagc aaaaaggcag
     1141 cgcaatgcgc tgccttttcg ttgatacgca ccgctgaatc ctatctgacg caatccacga
     1201 agtagcgttt cacgccatcg actttttccg cgaccagacc gtggatgtcg gtctcgaatc
     1261 ccggcaaggt ctgactgaaa tcgcgggcga atttaaggta atcgacgatg gtcttgttga
     1321 acatctcgcc ggggatcagc aacgggatac cgggcggata aggcgtcagc agcaccgcag
     1381 tgatgcgccc ttccagatcg tcgatctcca cgcgatcgat ctcgtcatgg gccattttgg
     1441 cgaacgcctc agacggcttc atcgccggct gcatgtccga cagatacatc tcggtggtca
     1501 tgcgcgcgac gttgtgctgc ttgtaggcat catgaatctg ctggcacaga tcgcgcaggc
     1561 cgatgcgttc gtaatgcgga tgcttggccg cgaattcggg cagtatccgc cagatcggcg
     1621 cgttcttgtc gtagtcgtcc ttgaactgct gcaacgcggt cagcagggta ttccagcggc
     1681 ccttggtaat gccgatggtg aacatgatga agaacgagta gagcccgcac ttctccacga
     1741 tcacgccgtg ctcggccaga tatttggtca ccatcgaagc agggatgccg ctgtcggcaa
     1801 aatcaccgtc cacatccagc ccgggcgtga tgatggtcgc cttgatcgga tcgagcatat
     1861 tgaaaccttc ggccagatcg ccgaagccat gccacttgtc ggaaggtttc agcacccagt
     1921 cttcgcgcga ccccatgcct tcctcggcga tcacatccgg gccccacacc ttgaaccacc
     1981 agtcctggcc gaactcttcg tccactttgc gcatcgcgcg gcggaaatcc agtgcctcgg
     2041 caatggattc ctcgaccagt gcggtgccgc ccggcggctc catcattgcc gccgccacgt
     2101 cgcacgaggc gatgatggcg tactgcgggc tggtcgaggt gtgcatcaga taggcttcgt
     2161 tgaagatatg cctgttcagt tgacggcttt gcggctcgcg caccagaatc tgcgaggcct
     2221 gcgacaggcc tgccagcaac ttgtgggtgg actgggtcgc aaagatcatc gactcgttcg
     2281 cgcgcggccg gtccttgccg atggcatgca tgtccttgta gaagtcgtgg aacgccgcat
     2341 gcggcagcca ggcctcgtcg aagtgcagcg tgtcgatgcg gccgtccagc atctccttca
     2401 gcatctcgac gttgtacacc acgccgtcat aggtgctctg cgtgatggtc aggatgcgcg
     2461 gcttcttggt cttgtccttg atgaaaggat tcgcatcgat cttgcgctgg atactcgcag
     2521 gctcgaactc cgacttcggg atcgggccga tgatgccgta gtggttgcgc gtcggcgtca
     2581 ggaacaccgg caccgcgccg gtcatcatga tggaatgcag aatggacttg tggcagttgc
     2641 gatccaccac gacgatgtcg tcgggcgcga cggtcgagtg ccacacgatc ttgttcgaag
     2701 tcgaggtgcc gttggtgacg aagaacagat ggtcggcatt gaaaatacgc gccgcattgc
     2761 gctccgaagc cgccacgggc ccggtgtgat cgagcaactg gcccagctca tccaccgcgt
     2821 tgcacacgtc ggcgcgcagc atgttttcgc cgaagaactg gtggaacatc tgccccaccg
     2881 gcgacttcag gaacgccacg ccgccggagt gaccggggca gtgccaggaa taggaaccgt
     2941 cctgcgcgta gtcgagcagc gcgcgaaaga acggcggcgc cagcgaatcc agataagact
     3001 tggcttcgcg gatgatgtgg cgcgcgacga attccggcgt gtcttcgaac atgtggatga
     3061 agccgtgcag ctcgcgcagg atatcgttcg gaatatgacg cgaggtgcgc gtctcgccgt
     3121 acagatagat cgggatatcg gcgttcttga aacgaatctc cgcgacaaag gcgcgcaaag
     3181 tcttcaacgc gacctcggtc tcctcctgac tacccgcgcc gaattcctcg tcgtcgatcg
     3241 acaacagaaa agccgaggct cggctctgct gctgcgcgaa cgaggtcagg tcgccgtaac
     3301 tggtgacgcc caggatttcc atgccctctt tctcgatggc atcggccagc gcgcggatgc
     3361 ccagaccgct ggcattctcg gaacggaaat cttcgtcgat gatgacgata ggaaacctga
     3421 acttcatgtt gcgctcctta acatagtcga acgtgtaaca aaatattgca gacctaaatc
     3481 gcgacaggcg aggtggaggt tcagtgaaaa ctcatatcgg ccacaggccg atgtgacgtc
     3541 ggaactaaag cgaaacttca tgttcagccc ctagaaaagg aagagtggag cgcgacgctt
     3601 cgcgcacagt ctgcacaact tgcagggttg agcttcagtg aagactcata ttggccacag
     3661 gccgatgtga cgtcggaact aaaacggaac ttcattgctt actcccagtg gggataacga
     3721 tttggcgcgg attgtcgcag atttcggggg tagcgtggcg cgatgttggg ccggtcgaaa
     3781 gggggtcaat cctgcggcgg aaaggaactt tccagcgaga cgaccagttc ggccagcacc
     3841 gcgcggaatt gctgttcgtc gcagcccatc agcaccgcgt cttccagcgc gtcccggcag
     3901 acctgacgga tctcgacgag gttctcctgc atcaccttgt tcttttcgat gcaggcgatg
     3961 acctctccct tgggggagcg ccagacgatt tgcgtgtcgt tgctcatatc ccgacaggcc
     4021 gattacatct tgggcagggt cacgccatgc tgcccctgat atttgccgcc ccggtccttg
     4081 taagaggtct cgcacacttc atccgactcg aagaacagca cctgcgccac gccctcgttg
     4141 gcgtagatct tggcgggcag cggcgtggtg ttggagaatt ccagcgtcac gtagccttcc
     4201 cattccggct cgaacggcgt gacgttgacg atgatgccgc aacgcgcata ggtggacttg
     4261 ccgaggcaga tggtcagcac gttgcgcgga atgcggaagt actcaacggt gcgggccaac
     4321 gcaaaggagt tgggcggaat cacgcagtaa tcggcctcga cattcacgaa ggaattcgga
     4381 tcgaagttct tcggatcgac gatggtgctg ttgatattgg tgaacagctt gaattcgcgc
     4441 gagcagcgga tgtcgtagcc gtagctcgag gtgccgtagg acacgatctt ctgaccgttc
     4501 tgctccttga cctgccccgg ctcgaacggc tcgatcatgc cgtgctgttc cgccatgcgg
     4561 cgtatccact tgtctgactt gatgctcatc gcggttccta aagggttatg cgggcgcgat
     4621 tttaccccat aaatcaggct tgttaatcgt cggcttccgg gctggcctcg cgcacctgat
     4681 tcagcaggtc ttcggcatga aactcggtct cggcgaagat catcttcacg acgtgcgggc
     4741 tggtggcagg aagttcggcg cgcaactgtt tcacccgtga agccgcctcg cgataggtat
     4801 cgtgctgctc cagcttgctc agcatcctga tcggctgctc ggtgatgcga taaataaaat
     4861 aggacatggg gtttactttt cttgcttgtc cataatgatc agagggcttc gccggccctc
     4921 gaaatcggca ttgtctgggt gggagcggct ttagccgcga ttgattcgct atgcacctca
     4981 cagcctatcg cggctaaagc cgctcccacc aatacatgct gccaggaatc aagcgggacg
     5041 cttcatgaat gtgaccacgc tggagccggg cggattcctg ctttcgcagc tgccctcacc
     5101 tgctcccgca taatactcgg cctcgtgcag cagattgatg acgtccacat agtgcttctg
     5161 cttcgccatc atcagcgcgg tgtagcctcc ctcgttgcgc gccagcggat ctgcgccggc
     5221 attcagcagc gtcgcgacca cttcggcctc gccataaccc gccgccagca tcagcggacg
     5281 cacgccgaaa ccgatggtcg cctcgatgtc agcaccggcg gcgatcaatg ccagtgtcac
     5341 ctcggcaaag ccgcgcttct gctcggcgtt atacaccgca caggctagag cagaccagcc
     5401 tcgttcgtcg gtcgcattga cgtctgcacc cgactcgatc aacgctttca ccgtcaccag
     5461 atcaccgttc ttcgcagcat cgaacaaatt catttttcct gcccctgagg atggaaccag
     5521 gccagtttct cgcgcagcgc caccacaccg ccgacgatga tcagtgtcgg cgcctgcggc
     5581 ttctcgcgct ccgcgattcc cggcagcgtc gccaatgtac cggtgaagac gcgctggttc
     5641 tgggtagtac cctgctgcac gatggcgatc ggcgtggtgt ccggcatacc gtgctcgatg
     5701 agcttgccgc acaggatatc cagaccatgc agccccatgt acaccacgac agtctgcttc
     5761 ggtcttgcca gcgcgtccca gtccagattc atcgtgccgt ctttcagatg gccggtgacg
     5821 aacatgcagg actgcgcgta gtcgcggtgc gtcagcggga tgcccgcata tgaagcgaca
     5881 ccggaagccg ccgtgatgcc gggtacgacc tgaaagggaa tgttctctgc cgccagcgtc
     5941 tcgatctcct cgccgccacg cccgaagatg aagggatcgc cacccttgag gcgcagtacg
     6001 cgcttgcctt ccttggccag acgcaccagc agctcgttga tctcctcctg acgcaaggtg
     6061 tgatcggcgc gctgcttgcc gacgaagatg cgctcggcat cgcgccgcgt catctcgacg
     6121 atgggcttcg acaccagatt gtcgtacacc accacgtcgg ccttctgcat caggcgcagc
     6181 gcgcggaagg tcagcagatc gggatcgccg ggaccggcac cgaccagata cacctcgccg
     6241 cgctggatgt tgtcttcgct ctccagcatc ttcagcaaca tcgcctcggc gctgctctcg
     6301 tcgccggtca gcaccttctc ggcgaccacg ccctcgaacg cgttctccca gaatatgcgg
     6361 cgtttggccg gattggtgac tcgcgtcttc accagttcgc ggaagcgctc ggcaaaggcg
     6421 gccagacggc tgtagttctg cggaatgacg gtctccagct tgccgcgcat catgcgcgtc
     6481 agcaccggcg atgcacctcc gctggagaac gccaccatca gc
//
LOCUS       DASUIV010000342         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_649002, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000342 DASUIV010000000
VERSION     DASUIV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649002"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..925)
                     /locus_tag="VFW59_12110"
     CDS             complement(41..925)
                     /locus_tag="VFW59_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5364998.1"
                     /translation="MLKLNRLLAATDLSAPARHAVERAALVSRQTGAPLDVLHVADLA
                     PLDKLRHLMGSSTDELRQQVLKSAQEKLHDLVVILKNHYGVSVGAHVAFGSLLAELDK
                     AVDALAAGLIVCGARGESFLRHILLGSTAERMLSRAKCPMLVVKQAAHEAYRTVLVPV
                     DFSPSSLRAIGNARLVAPQAEIVLLHAFDVPFEGHLRYAGVEEEVIRGYRTLARQEAV
                     QNLHALSAQAGLPPYEARLVVLQGLPSTHIIEQEQEVDCDLIVMGKHGESMLDEFFLG
                     SVTKHVLAESQCDVLVSV"
     gene            1088..1276
                     /locus_tag="VFW59_12115"
     CDS             1088..1276
                     /locus_tag="VFW59_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5364999.1"
                     /translation="MHSVVECCTIATTLACYVRENRVLSGHGHTGSMNLHLGSTAERL
                     LQCLPCDTLLVPSHRSTV"
     gene            1372..>2693
                     /locus_tag="VFW59_12120"
     CDS             1372..>2693
                     /locus_tag="VFW59_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5365000.1"
                     /translation="MLLASLILVPFAGSLLAALMPENARNREAWLAFAVTACGLLVAG
                     LLYPAVSAGTVLREEFAWLPSHGLDLVLRVDGLAWVFTMLVLVIGLLVVLYARYYMSP
                     KDPVPRFFSFLLAFMGSMLGVVLSGNLVQLVVFWELTSLTSFLLIGYWHHRADARRGA
                     RMAFTVTATGGLCLLAGVLVLGHIVGSYDLDEVLAAGDRIRAHALYRVALVLIALGAL
                     TKSAQFPFHFWLPHAMAAPTPVSSYLHSATMVKAGVFLLVRLWPALAGTAEWNWIIGG
                     AGVCTLLIGAFIAIFQHDLKGLLAYSTISHLGLITVLIGIGTPLAIVAAIFHILNHAT
                     FKASLFMTTGIIDHETGTRDLRVLRGLRHQLPVTAALAIVSSAAMAGVPLLNGFLSKE
                     MFFAETALVGGDEHWWMSIAAVAMGMFSVAYSLRFISIFFGTPTVAFPR"
BASE COUNT          427 a          884 c          824 g          558 t
ORIGIN      
        1 ccgactccct cgcgtgcttt gacctgcggc cgcggactgc tcacacggat acgagcacgt
       61 cgcactggga ctcggcgagg acatgcttgg tgacgctgcc gaggaagaat tcgtcaagca
      121 tgctctcgcc gtgtttgccc atcacgatca ggtcgcagtc cacttcctgt tcctgctcga
      181 tgatgtgcgt tgacggaagg ccttgcagca cgacaagccg tgcctcgtag ggcggcaatc
      241 cggcttgcgc gctcaaggca tgcagattct gaaccgcttc ctgccgggca agcgtgcgat
      301 agcccctgat cacttcttcc tctacaccgg cgtatcgcag atggccctcg aacggcacgt
      361 cgaatgcatg cagcaagacg atttctgcct gtggcgcgac aagccgcgca ttgccgatcg
      421 cgcgcagcga cgaaggcgag aaatccaccg gcacgagcac cgttcgatac gcttcatgcg
      481 ctgcctgctt gaccaccaac atcggacatt tcgcccggct gagcatccgt tccgcggtcg
      541 agcccagcag gatatgccgc aggaagctct cgccgcgggc accacaaacg atcaggcccg
      601 ctgccaacgc atccacggcc ttatccagtt ctgcaagcaa ggagccaaac gcaacatgag
      661 cgcccacgga cacgccgtag tggtttttga gaatgacgac aagatcatgc agcttttcct
      721 gtgcgctctt gagcacctgc tgtctcaact cgtccgtcga gctgcccatg aggtggcgaa
      781 gcttgtccag cggggcaaga tcggccacat gcaacacgtc cagcggggcc cccgtttgcc
      841 ggctcaccag cgccgcgcgc tcgacggcat ggcgcgccgg tgcggagagg tcggttgcgg
      901 caagcaggcg gttcagcttc aacatggtcg ttttcctttc cattcaaatg cctttcgcat
      961 cgacacgggc gacatcgatc gtgccgggtg cgtatcccgc atgggaaggt ggtctatgca
     1021 tgcttcggca tatcgttgcc gtaaaggttc tcggactgac cggcgatcac ttcccgaggg
     1081 caccctgatg cattctgtag tcgaatgctg cacgatcgcg acgacgctgg cgtgttacgt
     1141 acgcgagaat agggtgttga gcggtcatgg ccacaccggc agcatgaact tgcacctggg
     1201 cagcacagcc gaaaggttgc tccagtgctt accctgcgac acgctgctgg tgccgtcaca
     1261 tcgctccacc gtgtagattg agctgtcctg cgcacgttgc ggatggagga ccggtagcgc
     1321 caccccgccg ccccgcgcag actgactacc accatgccct gaccatacct gatgctgctt
     1381 gcttcactga tccttgtccc gttcgccggc agtctgctgg ccgccctgat gccggaaaat
     1441 gcccgtaacc gcgaggcatg gctggcgttc gccgtcacgg cctgcgggct gctcgttgcc
     1501 ggcctgctct acccagccgt ttccgccggc acggtgctgc gcgaggagtt cgcctggctg
     1561 ccctcgcacg ggctcgacct cgtgctgcgt gtggatggcc tggcctgggt attcaccatg
     1621 ctggtcctcg tcatcggcct gctggtggtg ctgtacgcgc gctactacat gtcgccgaag
     1681 gacccggtac cgcgcttctt ctccttcctg ctggccttca tggggtcgat gctgggcgtg
     1741 gttttgtcgg gcaacctggt gcaactggtg gtgttctggg agctcaccag cctgacctcg
     1801 ttcctgctga tcggctactg gcatcaccgc gccgacgccc ggcgtggcgc gcggatggcg
     1861 ttcacggtga ccgctaccgg cggattgtgc ctgcttgccg gcgtgctcgt gctcggtcac
     1921 atcgtcggca gctacgacct ggacgaagtg ctggctgcgg gggaccgcat tcgtgcgcac
     1981 gcgttgtacc gggtggcgct ggtgttgatt gcgctggggg cgctgaccaa gagcgcccag
     2041 ttcccgttcc acttctggct gccgcatgcg atggccgcac ccacgcccgt atcgtcctat
     2101 ctccactcgg cgacgatggt gaaggcgggc gtcttcctgc tcgtgcggct gtggccggca
     2161 ctggcgggaa ccgcggaatg gaactggatc atcggcggtg ccggggtgtg caccttgctg
     2221 atcggcgctt tcatcgcgat cttccagcac gatctcaagg gtctgctcgc ctattcgacg
     2281 atcagccatc tcgggctgat cacggtgctg atcggcatcg gcacgcccct ggcgatcgtc
     2341 gcggcgatct tccacatcct caatcacgcc accttcaagg cttcgctgtt catgaccacc
     2401 ggcatcatcg accacgaaac cggaacccgc gatctgcgcg tcctgcgcgg actgcgtcat
     2461 cagctgccgg tcaccgcggc actggccatc gtctccagcg ccgccatggc tggggtgccg
     2521 ctgctgaacg gcttcctgtc caaggagatg ttcttcgctg aaacggctct ggtgggcggc
     2581 gacgagcact ggtggatgtc catcgcggcc gtggcgatgg gcatgttcag cgtcgcctat
     2641 tcgctgcgtt tcatctcgat cttcttcggc acgcccacgg tcgcattccc gcg
//
LOCUS       DASUIV010000343         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_833697, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000343 DASUIV010000000
VERSION     DASUIV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833697"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..553
                     /locus_tag="VFW59_12125"
     CDS             164..553
                     /locus_tag="VFW59_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5365001.1"
                     /translation="MPRIDTGKLSARMRRGEVFSVECPSRDILKHVTSRWGVLVLVAL
                     MEGTHRFSELRRKVGGVSEKMLAQTLQQLEQDGFVDRVSHPVVPPHVEYSLTPLGIGV
                     GKQVAALTDWIETNLPKIMKERQKRSA"
     gene            1063..2229
                     /locus_tag="VFW59_12130"
     CDS             1063..2229
                     /locus_tag="VFW59_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5365002.1"
                     /translation="MALSQHRTSWLRYVSWVAGLVLLGLAAWFLSRPAPPRVPLVKVE
                     RGVVEASVSNTRAGTVKACHRAKLAPPAGGQIAHLLVKKGERVKARQVLLELWNEDLQ
                     AQAKLAEQQLSSASVRSVEVCALAEMSRKTAERAQQLRESGFISPEGLERAEADAKSK
                     QASCTVARGEIAQANSRIALAQAGLRRMVLRAPFDGIVADISGELGEYATPSPPGIPT
                     PPAIDLIDDSCLFVSAPIDEVDAAMVRRGLTARITLDAIKGKSFEGKVKRVAPYVIDL
                     EKQARTVEVEVNFTEPPQAEVLLVGYSADVEIIHTLHEKVLRIPTQALLEGKRVLAYR
                     ANDGVLEERVVKTGLANWEYTEIVSGLQEGDLIVTALDQPGIKAGARVQPAEGR"
     gene            2252..2935
                     /locus_tag="VFW59_12135"
     CDS             2252..2935
                     /locus_tag="VFW59_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5365003.1"
                     /translation="MIELQNVCRIFTVGDQQVAALRDIDLKLGAGEYISIMGPSGSGK
                     STLLNLLGLLDRPTSGTYRLEGGNVTDLDDEQQARVRSEKIGFVFQSFHLVPRLTAAM
                     NIELPMILAGIDIEERKTRVRRLLDNYGLADRADHRPDQLSGGQRQRVAIARATVMNP
                     GVLLADEPTGNLDRTTGWEVMKLLEQLVEQGITLVIVTHDAEIGSRAKRQIHMLDGRI
                     LSDEQRGEG"
     gene            2952..>3179
                     /locus_tag="VFW59_12140"
     CDS             2952..>3179
                     /locus_tag="VFW59_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5365004.1"
                     /translation="MKPGDILQFAFGSLRGSPTRTLLMMLAMSIGVAAVVVLTALGEG
                     ARRYVVNQFSSLGTNLVIVLPGRTETAGIGPG"
BASE COUNT          591 a          900 c         1045 g          643 t
ORIGIN      
        1 gacttttttc agcagcgatt ggatgacgag gcggcccagt tgtccggtgg ctccggtgac
       61 gacgatcatg atgtttcctt tcttcgttgg ttcgttaagt tcgggatgcg cagaatatcc
      121 gttacaatta ctttttgtaa gtacgcacaa aaaggtaagt gccatgccac gaatcgatac
      181 cggaaaactg tcggccagaa tgcggcgcgg ggaggtcttt tctgtcgaat gcccttcacg
      241 cgatatcctc aagcatgtaa ccagccgctg gggggtgctg gtgctggtgg ctttgatgga
      301 ggggacgcac cgtttcagcg aactgcgccg caaggtgggc ggggtgagcg aaaagatgct
      361 ggctcagacg ctgcagcaat tggagcagga cggttttgtc gaccgcgtat cgcacccggt
      421 ggtaccgccg cacgtggaat actcgttgac cccgctgggc atcggggtag gcaaacaggt
      481 tgcagcgctg acagactgga ttgaaaccaa cctgccgaaa atcatgaagg aacggcagaa
      541 gcgatctgcc tgatttgcct gcccgctttc ccgcagatga ctatctcgcg ttagctttga
      601 attgccgccg gtactgcgct ggagacaatc ccgtgatgcg cttgaacaag cgccggaacg
      661 acgacgcatc gccgtacccc acctgctcgg tgatcgtctc gaaatccgag ttcgacacct
      721 cctgtcattc cgctgcgccg ctctcgcatc tcgtaaagcg tgatggattc ccccgctaca
      781 gggcttgcaa acctgccgcg cagcattttg ctttcagtta tcattacccg tgctcgatcc
      841 caacctgggt attaaaggtt tggccgaccg gcgcgtgtcc tttttcggat cgcatgtgat
      901 gctgaaaatc gtaacgccga tgccggtgtt cgggaatggt ttgattgcaa cgcgccgggg
      961 ggtttatgct gctcttccgc tagtatgcat atcgtgtaac ccccccggtt gcgcggggat
     1021 tggttcagtt tgtttggtgc gcattattcc acaggtgaaa gcatggcctt atcgcagcat
     1081 cgcacttcat ggcttcgtta tgtttcatgg gtcgcaggac tggttctgct cggtctggca
     1141 gcatggttcc tgtcgcgtcc ggctcctccg cgagtgccgc tggtaaaggt cgagcgcggc
     1201 gtggtggagg cgtcggtgag caacacccgc gcgggtacgg tcaaggcttg ccatcgcgcc
     1261 aagctggcac cgccagcggg cgggcagatc gcgcatctgc tggtgaagaa gggcgagcgg
     1321 gtcaaggcgc gccaggtatt gctggagttg tggaatgagg acttgcaggc acaggctaaa
     1381 ttggccgagc agcagctgag ttcggcttcg gtgcgcagcg tagaggtgtg cgcgctggcg
     1441 gagatgagcc gcaagactgc ggagcgggcg cagcagttgc gcgagagcgg cttcatttcg
     1501 ccggagggac tggagcgtgc cgaggcggat gccaagtcca agcaggcttc atgtacggtt
     1561 gcgcgcggcg agatcgcaca ggcgaattcg cgcatcgctc tggcgcaggc cggattgcgg
     1621 cgcatggtgc tgcgtgcgcc gttcgacggc attgttgccg acatcagcgg tgagttgggc
     1681 gaatatgcca ccccttcacc gcccggcatc ccgacgcctc ccgccatcga cctgatcgat
     1741 gacagctgcc tgttcgtcag cgcgccgatc gatgaggtgg atgcggccat ggtcaggcgc
     1801 ggcctgacag cacgcatcac gctggatgcg atcaagggca agtctttcga gggtaaggtc
     1861 aagcgcgtcg caccctatgt gatcgatctg gagaaacagg cgcgcaccgt cgaagtcgag
     1921 gtcaatttta ccgagccgcc ccaggcagag gtgctgctgg tcggctacag cgcggatgtg
     1981 gagatcatcc ataccttgca tgaaaaggtg ctgcgcatcc cgactcaggc gctgctggaa
     2041 ggcaagcgcg tgctggccta tcgtgcgaac gatggcgtgc tggaagagcg ggtggtgaag
     2101 accggattgg cgaactggga atacaccgag atcgtttccg ggttgcagga aggcgatctc
     2161 atcgtgacgg cgctggatca gcccggcatc aaggccgggg cgcgggtgca accggcggag
     2221 gggaggtagg gggtgcccct gcgatgattc catgatcgag ctgcaaaacg tctgccgcat
     2281 atttaccgtt ggcgaccagc aggtcgcggc tttgcgcgat atcgacctga agctgggcgc
     2341 gggcgaatat atttccatca tggggccgtc cggctccggc aagtccacgc tacttaatct
     2401 gctcggtctg ctcgaccggc cgacttccgg cacctaccgg ctggaaggcg gcaacgtcac
     2461 cgatctcgac gacgagcagc aggcaagggt gcgcagcgaa aagatcggtt tcgtgttcca
     2521 gtcgtttcat ctggtgccgc gtctgacggc ggcgatgaac atcgagttgc cgatgatcct
     2581 ggcgggtatc gacatcgagg aacgcaagac ccgggtgcgg cgcctgctgg ataactacgg
     2641 tctggccgat cgcgccgatc accgtcccga ccagctttcc ggcggccagc gccagcgggt
     2701 ggcgatcgcc cgcgccacgg tgatgaatcc cggtgtgttg ctggccgacg agccgaccgg
     2761 caatctggat cgcaccaccg gctgggaagt gatgaagctg ctggaacaac tggtcgagca
     2821 gggcatcacg ctggtcatcg tcacccacga cgccgagatc ggcagccgcg ccaaacgcca
     2881 gatacacatg ctcgacggcc gcatcctttc ggacgaacag agaggagagg gatgatggtt
     2941 gacgttctaa catgaagccc ggcgacatcc tgcaatttgc cttcggtagt ctgcgcggca
     3001 gcccgacgcg caccttgctg atgatgctgg cgatgtccat cggtgtcgcc gccgtggtgg
     3061 tgctcaccgc gctgggcgag ggcgcgcgcc gctatgtcgt caaccagttc tcctcgctgg
     3121 gcaccaatct ggtgatcgtg ttgcccggtc gcaccgagac ggcgggcatc ggccccggc
//
LOCUS       DASUIV010000344         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_743502, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000344 DASUIV010000000
VERSION     DASUIV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743502"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..656
                     /locus_tag="VFW59_12145"
     CDS             12..656
                     /locus_tag="VFW59_12145"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5365005.1"
                     /translation="MNGIGMTSQRTRMRMIERLREQGIKDDAVLAAMFETPRHLFVDE
                     ALASRAYDDVSLPINFNQTISQPYIVARMIEILRNGKPLNRVLEIGTGCGYQASVLGQ
                     VAKDVYTVERIQPLYERARKQLRDLKQRNVSVRHADGTAGLPELGPFDGIIMACAAPS
                     LSAALREQLAVGGRMVLPVGMQEQWLYLVERDERGFRETRLEPVKFVPLLLGKT"
     gene            653..1552
                     /locus_tag="VFW59_12150"
     CDS             653..1552
                     /locus_tag="VFW59_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5365006.1"
                     /translation="MRTNRFLSGLVMAAILSGCASSGHRAPVTERGASASKSASAAES
                     ARNKAAREKDWRPRVHVVQKGDTLYSIAFNYGFDYHELAELNGINDPSRIQLGQEIRL
                     FPGARSDAATATKPSAPAIKDQPKLVKYAYSETAVAQIDKTDKPAPAATTAKPESKPK
                     AETKAAEEASHEIAPDADMEWNLPTQGKVISGFSESDNRKGVDIAGKLGQSINASAPG
                     KVVYSGSGLRGYGKLVIIKHNNTFLSAYAHNDKVLVKEGESVARGQKIAEMGNSDADQ
                     VKLHFEVRRFGKPVDPTKYLAFK"
     gene            1621..2475
                     /locus_tag="VFW59_12155"
     CDS             1621..2475
                     /locus_tag="VFW59_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5365007.1"
                     /translation="MSDTKLSTTNHDRDSAELRYVYPVVSRRAGGVSVGINLNTNNAC
                     NWRCLYCQVPDLTLGTAPPVDLGLLERELRGFLNELLHGDFMQTRVPEEARRINDIAL
                     SGNGEPTSAAEFAQVIELIARVRRELSLPAEIKTVLITNGSLLYRDDVQRGLRGMAGI
                     NGEVWFKLDRASEAGMQRVNDTRITMDKVRDNLIAAIAACPTWLQTCWFALDGVPPTA
                     QDEADYLEFVGELLRAGHKLQGVLLYGLARASLQPEAARLSNLPEAEMRRFAERIRQL
                     GLEVKVSA"
     gene            complement(2472..>2678)
                     /locus_tag="VFW59_12160"
     CDS             complement(2472..>2678)
                     /locus_tag="VFW59_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5365008.1"
                     /translation="IMSVLTMDMSSYEVERSKAPGYGDEMLNAARVPELGLREVCSEP
                     VELPPAMSFANVDEFLNRMRRYQR"
BASE COUNT          531 a          832 c          859 g          456 t
ORIGIN      
        1 tgagcacccg cctgaacggt atcggcatga cctcgcagcg caccaggatg cgcatgatcg
       61 agcgtctgcg cgagcagggc atcaaggacg atgcggtgtt ggcggcgatg ttcgagacgc
      121 cgcgccatct gttcgtcgac gaggcgctgg caagccgcgc ctatgacgac gtgtcgctgc
      181 cgatcaattt caaccagacc atctcccagc cctacatcgt ggcgcgcatg atcgagatcc
      241 tgcgcaacgg caagccgctc aatcgcgtgc tggagatcgg caccggctgc ggctatcagg
      301 cgtcggtgct ggggcaggtg gcgaaggacg tctataccgt ggagcgcatc cagccgctgt
      361 acgaacgcgc cagaaagcag ctgcgcgatt tgaagcagcg caacgtcagc gtgcgccatg
      421 cggacggcac ggcggggctg cccgagctgg ggccgttcga cggcatcatc atggcctgtg
      481 ccgcgccttc tctgtcggcc gcgctgcggg agcagctggc ggtcggtggt agaatggtgc
      541 tgccggtcgg gatgcaggaa cagtggctgt atctggtcga acgcgacgaa cgcggattcc
      601 gcgagacgcg tctggaaccg gtgaaattcg taccactgct gttggggaag acatgagaac
      661 aaatcgattt ttgtcgggat tggtgatggc ggccatcctg tcaggctgcg cctcgagcgg
      721 gcatcgtgcg cccgtgaccg agcgcggcgc ttccgcttcg aagtcggcca gtgccgccga
      781 gtctgcccgc aataaagcgg cgcgcgagaa ggactggcgt ccgcgcgttc acgtggtgca
      841 gaagggcgac accctgtaca gcatcgcctt caactacggt ttcgattacc acgaactggc
      901 cgaactgaac gggatcaatg acccgagccg gatccagctc ggtcaggaga tacgcctgtt
      961 ccccggtgcg cgttccgatg ccgctacggc gactaaaccg agtgctcccg cgatcaagga
     1021 tcagcccaag ctggtgaaat acgcctacag cgaaactgct gtggcgcaaa tcgacaagac
     1081 cgacaagcct gctccggccg cgacgaccgc caagccggag agcaagccga aagccgaaac
     1141 caaagctgcc gaagaagcca gtcatgaaat cgctcccgat gcggacatgg agtggaacct
     1201 gccgacacag ggcaaggtga tcagcggatt ctccgaatcg gataaccgca agggcgtcga
     1261 tatcgcgggc aagctgggcc agagcatcaa tgccagcgcg ccgggcaagg tggtgtacag
     1321 cggcagcggc ctgcgcggct acggcaagct ggtcatcatc aagcacaaca acaccttcct
     1381 cagcgcctac gcgcacaacg acaaggtgct ggtgaaagaa ggggagagcg tcgcgcgcgg
     1441 tcagaagatc gccgagatgg gtaactccga tgccgatcag gtcaagctgc acttcgaggt
     1501 gcgtcgcttc ggcaagccgg tcgacccgac gaaatatctg gccttcaagt aggggcacgt
     1561 aaaacatttg ttccggcacc ggcacggcgt accgtgcccc tgcgatttct tggggctttc
     1621 ttgagcgaca caaaactcag caccaccaat cacgaccgcg acagcgcgga gctgcgctac
     1681 gtctacccgg tggtgtcgcg ccgggctggc ggtgtttcgg tcggcatcaa cctcaacacc
     1741 aacaacgcct gtaactggcg ctgcctctat tgccaagttc ccgatctgac gctgggcacg
     1801 gcgccgcccg tcgatctggg tctgctggag cgcgagttgc gcggctttct gaatgagctg
     1861 ctgcatggcg acttcatgca gacccgcgtg ccggaagagg cgcgccgcat caacgacatc
     1921 gccctgtccg gcaacggcga gccgaccagc gccgccgaat tcgcccaggt catcgaactc
     1981 atcgcccgcg tgcgccgcga gctatccctg ccggccgaga taaagaccgt gctgatcacc
     2041 aacggcagcc tgctctatcg cgacgatgtc cagcgcggtc tgcgcggcat ggcgggaatc
     2101 aacggggaag tgtggttcaa gctggaccgc gcgagtgagg cggggatgca gcgcgtcaac
     2161 gacacccgca tcaccatgga caaggtgcgc gacaacctga tcgccgcgat tgctgcctgt
     2221 ccgacctggc tgcagacctg ctggttcgca ctggacggcg ttccgcccac cgctcaggac
     2281 gaggcggatt atctggaatt cgtcggggag ctgttgcgcg cagggcacaa gctgcaaggg
     2341 gtgctgctgt acggcttggc gcgcgcttcc ttgcagcccg aagccgcgcg cctgtccaat
     2401 ctgccggagg cggagatgcg gcgcttcgcc gagcgcatcc gccagttagg tctggaagtt
     2461 aaggtctcgg cttagcgctg gtaacggcgc atcctgttca gaaactcgtc cacattcgcg
     2521 aacgacatcg cgggaggcag ttcgacaggt tcggagcaca cttcgcgcaa tcccagctcg
     2581 ggaactcgag ccgcgttcaa catctcgtcg ccatacccgg gagccttgct gcgttcgacc
     2641 tcgtagctgc tcatgtccat cgtcagtacg ctcatgat
//
LOCUS       DASUIV010000345        12207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1021626, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000345 DASUIV010000000
VERSION     DASUIV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12207
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021626"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFW59_12165"
     CDS             complement(<1..437)
                     /locus_tag="VFW59_12165"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX5365009.1"
                     /translation="MYRYDKYDHQLTAERVAQFSDQVRRRLSGELSEDEFRILRLQNG
                     LYHQVHGYMLRIAVPYGLLGSEQVRTLAHIARKYDRGYGHFTTRQNIQFNWIKLADIP
                     EIFAELAKVEMHAHQTSGSCLRSITSDEFAGVARDEIVDPRPYA"
     gene            complement(453..668)
                     /locus_tag="VFW59_12170"
     CDS             complement(453..668)
                     /locus_tag="VFW59_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5365010.1"
                     /translation="MSTNKPNTPKQVSWFNGCGGRVGVVVGQDGEHAYIGMALCHDED
                     ADVAHILKFGAKFPLEAALLLPVSKSY"
     gene            complement(678..1463)
                     /locus_tag="VFW59_12175"
     CDS             complement(678..1463)
                     /locus_tag="VFW59_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5365011.1"
                     /translation="MDGLYTVSGFLVGLIVGVTGVGGGSLMTPLLVLVFGVSPATAVG
                     TDLLYASLTKTLGGWVHSKRGTVDWKVFGLLAMGSLPAAVVTIALLKYLALDEKTLRG
                     LVTSVLSVALLSTATALLFKDGIKKLARRKDGTVYELHHRYLPAATIATGVVVGALVT
                     VSSIGAGVLGTVALLFLYPRMPSVRVVGTDIVHAVPLTAVAGMGHMALGTVDLVLLAS
                     LLLGSLPGIYIGSHLSAKVPEKVLRPVLAIMLLIIGLKMVFYK"
     gene            1572..2498
                     /gene="cysB"
                     /locus_tag="VFW59_12180"
     CDS             1572..2498
                     /gene="cysB"
                     /locus_tag="VFW59_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HEX5365012.1"
                     /translation="MNLQQLRYLTEVVKRGLNISDAANALYTSQPGVSKQIKLLEEEL
                     GIDIFVRNGKRIVAVTEPGQAVLEIAQRVLHETESLKQVGQEFRSQDSGHLTIATTHT
                     QARYALPRVVQQFTQRYPGVKLGLHQGSPTQIAEQILDGSADIGIATESLALYDELVT
                     LPCYEWHHCVVVPPKHPLLDEKRLTLKKLAQYPIITYDFAFTGRNKINQAFTDAGIEP
                     NIALTAIDADVIKTYVELGMGIGIVAQMAFIPDRDKHLRMIDAGHLFQPSTTRIAIRK
                     NQYLRGFGFHFIELFAPHLTRDVVSQALHLTK"
     gene            2505..3371
                     /locus_tag="VFW59_12185"
     CDS             2505..3371
                     /locus_tag="VFW59_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HEX5365013.1"
                     /translation="MKTPKRIEPLIEDGLVDEVIRQLMSGKEATVYVVRCGDETRCAK
                     VYKEANKRSFRQSVDYTEGRKVKNSRRARAMEKGTSYGRKAQEEAWQNAEVDALYRLA
                     AAGVRVPTPYNFHEGVLLMELVTDAEGNAAPRLNDIQLSAEVSREYHGKLINQVVRML
                     CAGIVHGDLSEYNVLVDAEGPVIIDLPQAIDAAANNNALPMLLRDVENLASYFGQFAP
                     ELLDTDYGNETWSLYQGSQLHPECVLTGRFERIEKAVDLNSVMREINDTLKEEEARQQ
                     AIALRASRARTV"
     gene            complement(3703..4491)
                     /gene="phnE"
                     /locus_tag="VFW59_12190"
     CDS             complement(3703..4491)
                     /gene="phnE"
                     /locus_tag="VFW59_12190"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX5365014.1"
                     /translation="MHRPSPPVMGWSAALLLAGLLLLVVASFCSLTLQWHALFTADAL
                     RSALEFLNGFAPPELARPFLMKIAEGALETLSMSALGTAIAFFLGLMLALPASGRMGT
                     PARLLVRLVLNLLRSIPELVWAVILLIAAGLGPFAGTLALAVHTTGVLGRLFADALEN
                     LPPATEATLRINGATPMTAFFYAALPQISPQLMSYTLYRWENNIRAAAILGVVGAGGL
                     GQMLKYHLSLFQMQQAASIIIAMLLMITLVDGASFWLRRRLTGN"
     gene            complement(4493..5329)
                     /locus_tag="VFW59_12195"
     CDS             complement(4493..5329)
                     /locus_tag="VFW59_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5365015.1"
                     /translation="MAVNAASIGHPDPAWRGRVTVTAVALILLWPMLVASEFKPWILL
                     DEQSLGATGHFLAGFFPPAHSREFLGLVVEATWQTIAIATAGLTLALLGAIPLTLLAT
                     ESLSVSRLESGRMRPAGAVVRQLARWLLVLLRSVPELVWALLFVRIVGLGPTAGVLAI
                     ALTYSGMLGKVYAEILESAESHATESLLKNGSGRLAALFYGVLPASAAELLSYTTFRW
                     ECAIRGSVVMGFVGAGGLGQRMDESMKMLSGDEVSTMLFIFVLLVAGADLVSSSLRRR
                     LN"
     gene            complement(5342..6163)
                     /locus_tag="VFW59_12200"
     CDS             complement(5342..6163)
                     /locus_tag="VFW59_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5365016.1"
                     /translation="MSFVLRGVSVRHAAAECGSSALALHDINLSVAQGEQVALIGPSG
                     AGKTTLLHMLACAHRAASGEFSALGVAPWKLKHAERHALRARLFLAPQTPPLPPRQRV
                     VTAVLAGRLPQWTFRQAIASLIRPSEPDAAFDALARFNLQDKLYARVDRLSGGERQRC
                     GLARLLVSGADAFLVDEPLSALDPALSLQTLATLQQEASSRNATLVCSLHQVELALGH
                     FTRIVGLRDGRVVFDAPRAGVTEAMVAALYRNAQHADESQPLAPQRARTCISAPC"
     gene            complement(6166..7083)
                     /locus_tag="VFW59_12205"
     CDS             complement(6166..7083)
                     /locus_tag="VFW59_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5365017.1"
                     /translation="MTYLQQRDDSINASTTFSSKQTLGRIINAIMAAALLLSSGLSLA
                     QPVLRVSAIPDESPTELQRKFKPLGEYLEKKLGMKVEFTPVTDYAASVEGLINNKLDL
                     VWFGGFTFVQAKVRSNNQVTPLVQREEDEKFKSVFITTRNDITKLQDLKGKTFTFGSE
                     SSTSGHLMPRSYLLAANINPETDMKRIAFSGAHDATVAAVAGGKVDAGALNISVWEKL
                     LAQGKVDTKAVHVFYTTPGYYDYNWTVRADMNPVLRDKLARAFLALDKNNPQDKEILD
                     LQRATRFIPTKAENYGAIETAARSAGLLK"
     gene            complement(7445..8350)
                     /locus_tag="VFW59_12210"
     CDS             complement(7445..8350)
                     /locus_tag="VFW59_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEX5365018.1"
                     /translation="MWFKNLLLYRLTEWSVTPDALEEKLAQHILQPCSGLEMQSRGWV
                     SPKADGEPLVHVLGSQMLISLGLEKKLLPSSVINQFVKSRAVEVEEQQGYKPGRKQMK
                     ELKESITDELLPRAFAIRSKTCAWIDTDNMLLVIDAANYGKADELVEMLVKSVDGIAF
                     SLIRTQTSASSAMTAWLAGDDFPAGFTIDRDCELRGTGEERATVRYVRHALEVDEINK
                     HIKSGKEVTKLAMSWNDKLSFVLHENLQIKRLAALDLLKEQADNSDKDDLFDADFVMM
                     TGELRKLLPDIIEALGGERTTGIGG"
     gene            complement(8514..11441)
                     /locus_tag="VFW59_12215"
     CDS             complement(8514..11441)
                     /locus_tag="VFW59_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5365019.1"
                     /translation="MPDKTKEVRSYFAIALLVCGLALAAWFGERLNNEQSVRASRVVA
                     QDQLGMIRSRLEGSLYSDIQLVKGLVSVIAANPGLGQAQFSRAAKPLFDGSTNLRNIG
                     AAPNMVIRMMYPVAGNEKAIGLDYRNTPSQFEMAEKARTTGKMILAGPLVLAQGGTGI
                     IGRIPVFTEDPSGRQRFWGLISAVIDADKLYLSSGMQDADSMLEIAIRGKDGTGSQGE
                     VFFGRPELFDADPVLTDLALPVGSWQIAAVPRNGWSAQAGNVWELRLIFLLVGLLIVL
                     PVLALIRSLGALSASEFRFRQMFENHAAIMLLVDPESGEIIDANPAAANFYGYPLKSF
                     RGMNIDRINSSSEAEIQQSMHRAFRENKNYFVFEHRLADGSSRTVEVYSSPMISNNKK
                     LLFSIIHDISIRKQAEMDLRVVATAFESQEGILITDAHSKILRVNNSFTEITGYSADE
                     VLGKNPRILNAGLQDAEFYRSMWDSLLKEGSWAGEVWNRRKSGEIYPEYLAITAVKNE
                     EGVVTNYVASLADISQRKESEEKIRSLAFYDPLTRLPNRRLLLDRLQQALVSSARSSH
                     RGALLFLDLDNFKSLNDTLGHDIGDVLLQQVAQRLASCVREGDTVARLGGDEFVVVLE
                     DLSKQTPEAAAQTEDVCEKILANLNQPYQLGVHEYACTSSIGATLFNGYESEVENLLK
                     QADIAMYQAKKAGRNNLRFFDPAMQSMVTAHVALEADLRRAVSEQEQFQLYYQPQVDS
                     AGHILGVEALVRWHHPERGMVSPAEFIPLAEESGLILPLGHWVLITACGQLAGWAHSP
                     ETAHLTMAVNVSAKQFALPTFVEEVLTLVEHFRIDPARLKLEITESMLAANVDELVSK
                     MTVLKASGINFSLDDFGTGYSSLQYLKRLPLDQLKIDQSFVRDIVEDNSDRAIVKTII
                     AMARSMDLDVIAEGVETEEQRNLLLHKGCSRFQGYLFCKPVPIEQFEALLKRGGSQAA
                     AVQ"
     gene            complement(11639..>12207)
                     /locus_tag="VFW59_12220"
     CDS             complement(11639..>12207)
                     /locus_tag="VFW59_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX5365020.1"
                     /translation="MIAVPLLSTFFQRGAFSSGDVLMTSHALIGYSVGLIGMILVKIL
                     APGFYARQDIRTPVKIGIVTLIATQVMNVLFVFGMQLHHAGLALSIGLGACFNSAILF
                     HFLRKHGIYQPEPGWAKFFLKVLAALLALGLALWFGMGSEQSWLTGGGWTRILRLSAL
                     VAGGIAVYFAVLFALGFRPRDFSRRAVH"
BASE COUNT         2672 a         3790 c         3527 g         2218 t
ORIGIN      
        1 gcatagggac gcggatcgac gatctcgtcg cgtgcgacgc cggcgaactc gtccgaggtg
       61 atgctgcgca ggcagctgcc ggaagtctgg tgcgcgtgca tctccacctt cgccagctcc
      121 gcgaagattt ccggtatatc cgccagcttg atccagttga actggatgtt ctggcgcgtg
      181 gtgaagtggc cgtagccacg gtcgtacttg cgtgcgatgt gcgcgagggt gcgcacctgc
      241 tccgaaccca gcagtccgta gggcaccgcg atgcgcagca tgtagccgtg cacctggtga
      301 tacaggccgt tctgcaggcg caggatgcgg aactcgtcct cgctcaactc gccggacagg
      361 cggcggcgca cctggtcgct gaactgcgcg acgcgctcgg cggtgagctg gtgatcgtat
      421 ttgtcgtagc ggtacatgct tgcctcctgc cctcaataac tcttagaaac cggcagcagc
      481 aaggctgctt ccagcgggaa cttcgcgccg aatttcagga tatgcgcgac atctgcatcc
      541 tcgtcatggc acagcgccat cccgatgtag gcatgctcgc cgtcctggcc cacgaccacg
      601 ccgacgcgtc cgccgcagcc gttgaaccag ctgacctgtt tcggtgtatt cggtttgttt
      661 gtgctcatct gcttccccta tttgtaaaac accatcttca gaccgattat cagcaacatg
      721 atcgccagca ccggacgcaa caccttctcc ggcaccttcg cgctcaaatg gctgccgata
      781 tagattccgg gcaacgaacc cagcagcagg ctggccagca gcaccagatc caccgtcccc
      841 agcgccatgt gccccatccc ggccacggcc gtcagcggca ccgcatgcac gatatccgtg
      901 ccgaccaccc tgaccgaagg catgcgcgga tacaggaaca gcagcgcgac cgtgcccagc
      961 acaccggcac cgatggacga gaccgtcacc agcgcaccga caaccacgcc ggttgcgatc
     1021 gtcgcggccg gcagatagcg gtgatgcagc tcgtacaccg tgccgtcctt ccggcgcgcc
     1081 agcttcttga tgccgtcctt gaacagcagc gcggtggcgg tcgacagcaa ggccacgctc
     1141 agcacgctgg tcaccagacc gcgcagggtc ttttcatcga gcgcgagata tttcagcagg
     1201 gcgatggtga cgactgcggc gggcagactg cccatcgcca gcaaaccgaa caccttccag
     1261 tccaccgtgc cgcgcttgct gtgcacccag ccgcccagcg tcttggtcag cgaggcgtac
     1321 agcagatcgg tccccactgc ggtcgcgggc gagacgccga agaccagcac cagcagcggc
     1381 gtcatcaacg agccgccgcc gaccccggtc accccgacga tcagcccgac cagaaaaccg
     1441 gaaacggtgt acagaccatc catctcaatc acccctttta ttcaacgagg cgcattctag
     1501 gcagggatcg ttatattcca aaatactttt atgttagtat cttataactg caaaacatat
     1561 agagacccgc catgaatctg caacaactgc gttacctgac cgaagtggtc aagcgcggcc
     1621 tgaacatctc ggatgcggcc aacgcgctat acacctcgca accgggagtg agcaaacaga
     1681 tcaagctgct ggaagaggag ctgggcatcg acatcttcgt gcgcaacggc aagcgcatcg
     1741 tggcggtgac ggagccggga caggcggtgc tggagatcgc acagcgcgtg ctgcacgaga
     1801 cggagagcct gaaacaggtc gggcaggaat tccgcagcca ggacagcggc cacctgacca
     1861 tcgccaccac gcacacccag gcgcgctatg cgctgccgcg cgtggtgcag caattcaccc
     1921 agcgctatcc cggagtgaaa ctggggctgc atcagggcag cccgacacag atcgccgaac
     1981 agatactgga cggctccgca gacatcggca tcgccaccga gagcctcgcg ctctacgacg
     2041 aactggtcac cctgccctgc tacgaatggc atcactgcgt ggtggttccg cccaaacatc
     2101 cactgctgga cgagaagcgc ctgacgctga agaagctcgc gcaatatccc atcatcacct
     2161 acgacttcgc cttcaccggg cgcaacaaga tcaatcaggc cttcaccgat gcgggcatcg
     2221 aaccgaacat cgcgctcacc gccatcgacg cggacgtgat caagacctat gtcgaactgg
     2281 gcatgggcat cggcatcgtg gcgcagatgg ccttcattcc ggatagggac aaacatctgc
     2341 gcatgatcga tgccggtcac ctgttccagc ccagcaccac gcgcatcgcg atccgcaaga
     2401 accagtacct gcgcggtttc ggcttccatt tcatcgagct attcgcaccc catctgaccc
     2461 gcgacgtggt gtcgcaggcg ctgcacctga ccaaataacc gccgatgaaa acaccgaaac
     2521 gcatagaacc cctgatcgaa gacggactgg tcgacgaggt catccgccag ctgatgagcg
     2581 gcaaggaagc cacggtctac gtggtgcgct gcggcgacga gacgcgctgc gccaaggtct
     2641 acaaggaagc caacaagcgc agcttccgcc agagcgtgga ttacaccgag gggcgcaagg
     2701 tcaagaacag ccgccgcgcc cgcgcgatgg aaaaaggcac gagctacggg cgcaaggcgc
     2761 aggaagaagc ctggcagaac gccgaggtgg atgccctgta ccggctcgcg gcagccgggg
     2821 tgcgcgtgcc cacgccgtac aacttccacg agggcgtgct gctgatggag ctggtcacgg
     2881 atgccgaggg caacgccgcc ccgcgactca acgacataca actcagcgct gaagtctccc
     2941 gcgaatacca cggcaagctg atcaatcagg tggtgcgcat gctgtgcgcg ggcatcgtgc
     3001 acggcgacct gtccgaatac aacgtgctgg tggatgcgga aggcccggtc atcatcgacc
     3061 tgccccaggc catcgacgcc gcagcgaaca acaacgcgct gccgatgctg ctcagggatg
     3121 tcgagaacct cgccagctac ttcgggcagt tcgcgcccga actgctggat accgactacg
     3181 gcaacgagac ctggtcgctc tatcagggca gccagctaca cccggaatgc gtgcttaccg
     3241 gaagattcga gcgcatcgag aaagcggtcg acctgaacag tgtcatgcgc gaaatcaacg
     3301 acacgctcaa ggaagaagag gccagacagc aggcgatcgc actgcgcgcg agtcgcgcac
     3361 gtacggtcta acgctggcac tgacgataga aagcggcggt ttccttaaag aaaccggccg
     3421 cacaatactt gcccaccacc cggccggttc gtattaagat gccggcgcaa caacacaagg
     3481 agaacgatca tggccaaagg tcagcaacgc aaaaccaaag aagcaaagaa acccaaaaaa
     3541 gcacccccac caaaaatctg acaaaacccc aaccctcacc tccgactgcc ggtcgtgagg
     3601 gttagtcagt tttccaggct gtcgaaaatc gacccgcctt tcccccgaat atcgccggaa
     3661 aatattgcgg cgatccccct gatttctgcc aaatcgcttc tgctagttac cggttaacct
     3721 gcgccgcagc cagaaactgg cgccatcgac gagggtgatc atcaacagca tcgcgatgat
     3781 gatgctggcc gcctgctgca tttgaaataa ggacaggtgg tatttcagca tctgccccag
     3841 ccccccggca ccgaccactc ccagaatggc tgcggcgcgg atattgttct cccatcgata
     3901 cagcgtgtag gacatcagct gcggcgaaat ctgcggcaac gccgcgtaga aaaatgccgt
     3961 catcggtgtt gcgccattga tgcgcagcgt ggcttcggtg gcaggcggaa gattctccag
     4021 cgcatcggca aacaggcggc caagcacccc ggtggtatgc accgccagcg ccagcgttcc
     4081 cgcgaagggc ccgagtccgg ccgcgatcag caggatgacg gcccaaacca gctcgggtat
     4141 cgaacgcaac agattgagca ccaggcggac cagcaagcgg gcaggtgttc ccatacgccc
     4201 gctcgctggc agcgccagca tgaggccgag gaaaaatgcg atggccgtcc ccagcgcgga
     4261 catggagagc gtttccagcg cgccctccgc gatcttcatc agaaaaggcc gggccaattc
     4321 cggcggcgca aatccgttca agaattccag cgcggaacgc aaggcatcgg ccgtaaagag
     4381 cgcatgccat tgcaacgtca gcgagcagaa gctggcaacg accagcaaca gcagcccggc
     4441 cagcaagagc gccgcagacc agcccatcac gggaggagag ggacgatgca tgtcagttca
     4501 gacgcctgcg caaagaagag ctgaccagat cggctccggc caccagcaac acgaagatga
     4561 acagcatggt agagacctca tcgccggaca gcatcttcat cgattcatcc atgcgttgac
     4621 cgagcccgcc tgccccgacg aagcccatca ccaccgagcc acggatcgcg cattcccaac
     4681 gaaaggtggt gtaggacagc aactcggcgg cggatgcggg caggactccg tagaacaggg
     4741 cggcgaggcg gccgctgccg ttcttcagca acgactcggt ggcgtgagac tccgcagact
     4801 ccagtatctc ggcgtagacc ttgcccagca tgccgctgta agtgagcgcg atcgccaaca
     4861 cgccggcggt cggccccaga ccgacgatgc gcacgaacag caatgcccag accagctcgg
     4921 gcacgctgcg cagcaacacc agcagccagc gggcaagctg cctgacgacg gcaccggcgg
     4981 gacgcatccg tccggattcc agacgcgaca ccgacaacga ttccgtcgcc agcaacgtca
     5041 gagggatcgc gcccagcaat gccagcgtca gcccggcggt ggcgatcgcg atggtctgcc
     5101 aggtcgcctc cacaaccagc cccagaaact cgcgggaatg cgcgggcgga aagaaaccgg
     5161 cgagaaagtg tccggtcgct cccaggcttt gttcgtccag caatatccac ggcttgaatt
     5221 cgctggcaac cagcatcggc cacagcaata tcagcgcgac ggccgtaacc gtcacacggc
     5281 cccgccaggc gggatcgggg tgacctatgg aagcggcgtt aacggccatg ttcatcgcct
     5341 gtcagcaggg ggcactgatg caggttcttg cccgttgcgg agcaagcggt tggctttcat
     5401 cggcatgttg cgcattccgg tataacgccg cgaccatcgc ctccgtcacc ccggcacgag
     5461 gggcatcgaa gacgacgcgt ccatcgcgca gaccgacgat gcgcgtgaag tggccgagcg
     5521 ccagctccac ctgatgcagg ctgcaaacca gcgtggcatt gcggctgctt gcctcctgtt
     5581 gcagcgtggc cagggtttgc agcgagagcg ccggatcgag cgcggaaagc ggttcgtcca
     5641 ccagaaacgc atcggcgccg gacaccagca ggcgagccag cccgcaacgc tggcgttcgc
     5701 cacctgacag acgatccacc cgtgcataca gcttgtcctg cagattgaag cgtgccagcg
     5761 catcgaatgc ggcatcgggt tcgcttggcc tgatcagcga tgcgatggcc tgccggaaag
     5821 tccactgtgg cagacgtccg gccagcacgg ccgtcaccac ccgttgccgt ggcggcagcg
     5881 gcggcgtctg gggcgcgaga aacaggcggg cgcgcagtgc gtgacgctcg gcatgtttca
     5941 gcttccaagg cgctacaccc aaagcggaga actcgcctga cgctgcccga tgggcgcagg
     6001 ccagcatgtg cagcagcgtc gtcttgcccg cgccggaggg gccgatcaac gcgacctgct
     6061 cgccctgcgc gaccgacaag ttgatgtcgt gcaaagccag tgccgagctt ccgcactcag
     6121 cggcggcatg gcgcacgctg acaccgcgca atacgaacga catgattatt tcagcaggcc
     6181 tgcgctgcgc gctgccgttt cgatcgcgcc gtagttttca gccttggtcg ggatgaagcg
     6241 cgtggcacgt tgcagatcga gaatctcctt gtcctgcggg ttgttcttgt ccagtgcgag
     6301 gaaggcacgg gccagcttgt cgcgcaacac cgggttcatg tcggcgcgca ccgtccagtt
     6361 gtagtcgtaa tagcccggtg tggtgtagaa gacgtgaacg gccttggtgt ccaccttgcc
     6421 ttgcgccagc agcttctccc ataccgagat attcaatgcg cccgcgtcga ccttgccgcc
     6481 ggccacggcc gccacggtcg catcatgcgc gcctgagaag gcgatgcgct tcatgtcggt
     6541 ttccgggttg atgttggcgg ccagcaggta ggaacgcggc atcagatggc ccgaggtgga
     6601 agactcggaa ccgaaggtga acgtcttgcc cttcaggtct tgcaacttgg tgatgtcatt
     6661 gcgcgtggtg atgaacaccg acttgaattt ctcgtcttcc tcgcgctgca ccagcggggt
     6721 cacctgattg ttgctgcgca ccttggcttg cacaaaggtg aagccgccga accagaccag
     6781 atccagcttg ttgttgatca gcccttccac cgaggccgca tagtcggtga cgggggtgaa
     6841 ttcgaccttc atgcccagtt tcttttccag atactcgccc agcggcttga acttgcgctg
     6901 caactcagtc ggcgattcgt cggggatggc agagacgcgc agcacgggtt gcgccagaga
     6961 caggccggat gacagcagca aagccgcagc catgatcgcg ttaatgatcc ggcccaatgt
     7021 ttgtttggaa gagaaagtcg tacttgcgtt gatggaatca tcgcgttgtt gcagataagt
     7081 catagcagcc ctttaaaaaa atagacaggg cgctaccgga tagcacgaga aaaacagact
     7141 gaagccaggt cgtccagaga gccaacggcc gcgtcgcggc agatcgcatc atgtccgatg
     7201 gagaacttcg cacctctaaa tagtctgctg acctgcacat tggcagcgct tggaaacggc
     7261 acattccgtc atgctcattc ttcccgccag ccacacacag gccgtctgac ccgatccagc
     7321 atcccctgcg ggagtgaagt cggcgttacg gcgtgccagt tggaagtgaa cacgacagcg
     7381 gcgcgcagtt taccagaggc taaggcgctt cgacgctgaa aatctcagct ggctccgggc
     7441 gagctcaccc accgatgccc gtggttcgtt cgccgccgag cgcttcgatg atgtcgggca
     7501 gcaatttccg cagctcacct gtcatcatga cgaagtccgc atcgaacagg tcgtccttgt
     7561 cgctgttgtc ggcctgttcc ttgagcaggt ccagcgcagc gagacgcttg atctgcagat
     7621 tctcgtgcaa cacgaaggag agcttgtcgt tccagctcat cgcgagcttg gtaacctcct
     7681 tgccggactt gatgtgtttg ttgatctcat cgacctcgag ggcatgccgg acgtagcgca
     7741 cggtcgcccg ctcctccccc gttccgcgca attcgcaatc ccggtcgatg gtgaatccgg
     7801 caggaaaatc atcgccggcc agccaggcgg tcatcgcaga ggatgccgag gtctgcgtcc
     7861 tgatcaggct gaacgcgatc ccgtcgaccg acttgaccag catctcgacc agttcgtccg
     7921 ccttgccata attggcggca tcgatcacca gcagcatgtt gtcggtatcg atccatgcgc
     7981 aggtcttgct gcggatggcg aacgcacggg gcagcaattc atccgtgatg gactccttga
     8041 gttccttcat ttgcttgcgc cccggcttgt aaccctgctg ctcttcgact tcgacggcgc
     8101 gcgacttgac gaactggttg atgaccgacg atggcagcag cttcttctcg agaccgagac
     8161 tgatcagcat ttgcgagccg agcacatgca ccagcggctc gccatcggcc ttgggcgaca
     8221 cccagccacg gctctgcatc tccagcccgg agcagggctg caggatgtgt tgcgcaagtt
     8281 tttcttcgag ggcgtcgggc gtgaccgacc attcggtcag acgatagagc agcagattct
     8341 tgaaccacat aaatttaaga ccaaagctaa gcagaaaggc gttcgattct atcgtctagt
     8401 caaactgcat tccagtggtc tcttggtctt tgacgacggg actgagcgaa acggtgtttt
     8461 ttgccggaaa cctcattgag agggctcatg gtcgagcagt ccgatgaaaa agctcattgg
     8521 acggcagccg cctgcgatcc gccgcgcttc agcaacgcct caaattgctc gatcggcacc
     8581 ggcttgcaaa acagataacc ctggaagcga ctgcaaccct tatgcaagag gagattgcgc
     8641 tgctcttccg tttcgacgcc ttccgcgatc acatccagat ccatgctgcg cgccatggcg
     8701 atgatggttt taacgatcgc ccggtcactg ttgtcttcga cgatatcgcg cacaaacgac
     8761 tgatcgattt tgagctgatc gagcggcagc cgtttgagat attgcagcga ggaatagccg
     8821 gtaccgaagt cgtccagcga aaagttgatg ccgctcgctt tcaaaacagt cattttggaa
     8881 acgagttcat cgacattagc ggccaacatg ctctcggtga tttccagctt gagtctggcg
     8941 ggatcgatcc tgaagtgttc gaccaatgtc agcacctctt cgacgaaggt cggcaaggcg
     9001 aactgcttgg cactgacatt cacggccatc gtgaggtggg cagtttccgg gctatgcgcc
     9061 cagcctgcca gttgcccgca ggcggttatc agcacccagt gccctaacgg caggatcaga
     9121 ccggactctt cggccagcgg gatgaattcg gcaggtgaca ccatgccacg ctcgggatga
     9181 tgccagcgca ccagcgcttc gacgccaagt atgtggcccg ccgagtcgac ttgcggctga
     9241 tagtagagct ggaactgctc ctgttcggaa acggcacggc gcaggtcggc ttccagtgcg
     9301 acatgagcgg tgaccatgga ctgcatggcg ggatcgaaga agcgcagatt gttgcggcca
     9361 gctttcttgg cctggtacat cgcgatatcc gcctgtttca gcaggttctc gacttccgac
     9421 tcgtaaccgt tgaacagggt cgcaccgata ctggaagtgc aggcatactc atgcacacca
     9481 agctgatagg gctgattgag gttggcgaga atcttttcgc aaacatcctc cgtctgcgct
     9541 gcggcttctg gcgtctgttt gctcaaatcc tccagcacca ccacgaactc gtcgccgccc
     9601 agtcgcgcca ccgtatcgcc ttcgcgcacg caagacgcca agcgctgggc aacctgttgc
     9661 agcagcacat caccgatgtc gtgaccgagc gtgtcattga gggatttgaa gttatccaga
     9721 tcgaggaaga gcagcgcgcc gcgatggctg ctgcgcgcgc tggaaaccag agcctgttga
     9781 agccgatcca gcagaagccg ccgattgggc agccgggtca gcggatcata aaaagccagg
     9841 ctcctgatct tctcctcaga ctccttgcgc tggctgatgt cggccagcga cgccacatag
     9901 ttggtgacga cgccctcctc gttttttact gcggtgatgg cgagatattc aggatagatt
     9961 tccccgctct tgcgccggtt ccatacttca ccagcccatg aaccttcctt gagcaggcta
    10021 tcccacatgg accggtaaaa ctcggcatcc tgaagtccgg cattgagaat gcgcggattc
    10081 ttgcccagaa cctcatcggc ggagtagccg gtgatttcgg taaatgaatt gttcacgcgc
    10141 aggatcttgc tatgggcgtc ggtgatcagg atgccttcct gggattcaaa ggcggtcgcg
    10201 acgacacgca gatccatttc ggcctgtttg cgtatgctga tgtcatgaat gatggaaaac
    10261 aataacttct tgttgtttga aatcatcggc gacgaataca cctcgacggt tcgggaggaa
    10321 ccatctgcca aacgatgctc gaaaacaaaa tagtttttgt tttcccggaa cgcccgatgc
    10381 atcgattgct gaatctccgc ctctgacgag gaattgatac ggtcgatatt catgccgcga
    10441 aatgacttca gcgggtatcc gtagaaattc gctgctgcgg gattcgcatc gatgatctcg
    10501 ccggattcag gatcgaccaa caacatgatc gcagcgtgat tctcgaacat ctgacggaag
    10561 cggaattcgc tggcgctcaa ggcaccgagc gagcggatca aggcaagaac aggcaggacg
    10621 atgagcaagc cgaccagcaa gaagatcaag cgcagttccc acacattacc tgcctgagcc
    10681 gaccagccat tgcgtggcac ggccgctatc tgccaggagc cgaccggcaa cgccagatcg
    10741 gtcagcaccg ggtcggcgtc aaacagttcg gggcgtccaa aaaacacttc gccctgcgag
    10801 cccgtgccat ccttgccgcg aatggcgatt tccagcatcg agtctgcatc ctgcatgcca
    10861 ctgctcagat agagcttgtc cgcatcaatc accgcagaaa tcagccccca gaatcgctgc
    10921 cggcccgacg gatcttcagt gaataccgga atacgcccga tgatacccgt ccccccttgc
    10981 gccaagacga gcggcccggc caatatcatc ttcccggtgg tgcgagcctt ttcggccatc
    11041 tcgaattggc tgggtgtatt gcgatagtcc agtccgatcg ccttttcgtt gccagcaacc
    11101 ggatacatca tgcgtatcac catgttcggg gctgcaccga tgttgcgaag gttggtgctg
    11161 ccatcgaaca gcggtttggc ggcacgcgaa aattgcgcct ggcccaaccc gggattggca
    11221 gcgatgacgc tcaccaatcc tttaaccagt tgtatgtcgc tatagagact gccctccaga
    11281 cgactgcgaa tcatcccgag ctgatcctgc gctacaacac gactggccct gacagactgc
    11341 tcgttgttca atctttcgcc gaaccatgcg gccagtgcca aaccacagac gagcagggca
    11401 atcgcaaaat agctccgaac ttccttggtc ttgtcaggca ttggcatata gggcgattaa
    11461 aagttgagtg ggacaacatt gctacttcgt cgcagcgaca cgaccagaac cttaagcatg
    11521 gttgagcgag caaataaaaa agcacatatt aaaaacgatc ctctgcgcaa cccatgatca
    11581 acggcaggct tggtctgaca ccgccgtcac ctgccaatgt ctgttatcgc cagcaagctt
    11641 aatgcacggc tcgcctggag aagtctctgg gacgaaatcc caatgcgaac agcacggcga
    11701 aatacaccgc gatgccgcct gcaaccagcg ccgacagacg caggatacgc gtccaaccgc
    11761 caccggttag ccagctttgt tcgctgccca tgccgaacca cagcgcgagc cccagcgcca
    11821 gcagtgcggc cagcaccttg aggaagaact tcgcccagcc gggttccggc tggtagatgc
    11881 cgtgcttgcg cagaaaatgg aacaggatag ccgagttgaa gcaggcaccg aggccgatcg
    11941 acaaggccag tccggcatga tgcaactgca tgccaaagac gaacagcaca ttcatcacct
    12001 gcgtggcgat cagggtcacg atgccgatct tcaccggggt gcgtatgtcc tgccgcgcat
    12061 agaagccggg ggcgagaatc ttcaccagta tcatgccgat caatccgacg ctgtagccga
    12121 tcagcgcatg gctggtcatc agcacgtcgc cggatgaaaa cgcgccgcgc tggaagaagg
    12181 tcgacagcag gggcacggcg atcatac
//
LOCUS       DASUIV010000346        15427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gallionella sp. isolate SMAG_U1469 k141_1022453, whole
            genome shotgun sequence.
ACCESSION   DASUIV010000346 DASUIV010000000
VERSION     DASUIV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961618
            Sequence Read Archive: SRR25158400
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gallionella sp. (soil metagenome)
  ORGANISM  Gallionella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Gallionellaceae; Gallionella.
REFERENCE   1  (bases 1 to 15427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,458
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,406
            CDSs (with protein)               :: 2,406
            Genes (RNA)                       :: 43
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15427
                     /organism="Gallionella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022453"
                     /isolate="SMAG_U1469"
                     /isolation_source="soil"
                     /db_xref="taxon:2052161"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.91833333 N 123.6008333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..769
                     /locus_tag="VFW59_12225"
     CDS             143..769
                     /locus_tag="VFW59_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HEX5365021.1"
                     /translation="MLDFTEYTKIFISLFAILDPIGIIPIIVLFTAEMTSSKRAQVGR
                     LASLTVCAILLAALLIGQPLLAFFGISINSFRVAGGILLLLMAIKMLMGNFYRSGKDD
                     QVGDETLSANAIVPLSTPLLAGPGSISAVIVEAHKADGLQHYLIMGLEIVLLSITVWL
                     TFLIAPWVAQRMGKLGINVFTRLMGLILAAISVEFIASGMRGLFPVLG"
     gene            886..2607
                     /gene="ycaO"
                     /locus_tag="VFW59_12230"
     CDS             886..2607
                     /gene="ycaO"
                     /locus_tag="VFW59_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HEX5365022.1"
                     /translation="MTETFIPSKDASLESSIRTLHAKLEAIGFHVEERSWLNEIENIW
                     SVHVSDRDCPRLFSNGKGGSELAARASALGEFFERLGTNYFWTHFYLGETLSHADFVH
                     YPNEQWFPVQGDKWPAALLTPELQKFYNPQGGVLAKQLIDLNSGNAERGICAIPYQRL
                     RDDKTVLFPVNLIGNLYVSNGMSAGNTLMEARTQALAEIFERDIKFRIIREGLCLPDV
                     PETVIARYPRIAAGIKGLREAGYGILVKDASLGGQYPVMNVTLLHPADQGCFASFGAH
                     PRFEVALERALTELLQGRAIDGLKDFPAPGFDMEEIADEQNLEIHFVDSSGVISWEFL
                     RDTPDFEFSDWNFGTTTEEDYRWCCDAIHASGHDIYVADFTHLDVYTCRIFVPGMSEI
                     YPIEELQWQNNTVGNLVRPALLRLPELDEEECNDLLDTLLDLDLADNLAVTTLIGLAA
                     DPGSVWMDLRVGELKALLALAVGDEDAILEGCDWIAQLGQLEDKRARVYRCISQLLQL
                     QEMADSTAPYEASLQLLFGADTLQLAQQLLSRETRFFGLGTLGANMEGSNMHTRLLEA
                     YRKVWKR"
     gene            complement(2672..3097)
                     /locus_tag="VFW59_12235"
     CDS             complement(2672..3097)
                     /locus_tag="VFW59_12235"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX5365023.1"
                     /translation="MKTSRIFGAILVLSAVLMGGCATTDSHSPSPTLAYGVVDGIEAT
                     RGSDGGIGAGTVIGGVVGGVLGHQVGGGTGNDVATVAGVVGGAVVGHQIEKSNRQADS
                     YRVRVRLENGGEQTVTQQSIDDLRIGDRVRIESGRVSRY"
     gene            3346..3798
                     /locus_tag="VFW59_12240"
     CDS             3346..3798
                     /locus_tag="VFW59_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5365024.1"
                     /translation="MRGWLNKLCCLSLIISGPAAAAPGNFSSVIGSALVCNDQVNSAY
                     FNGYMKQFFGAPAFTAGGANWWKVNDSLFNAPVEYVFVGTGHDFIGATFRTRPAELVV
                     NIRDAMGIEYKQIRAETWVSPAAGALITYHDKNTPSKMYCIGSPHTAY"
     gene            3963..5504
                     /locus_tag="VFW59_12245"
     CDS             3963..5504
                     /locus_tag="VFW59_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HEX5365025.1"
                     /translation="MTHNFDDLQPRPVWAHFATLCAIPRPSHHEAALIAHLIGWAQAR
                     GISAGVDGAGNLILKKPASPGCESMPGVILQGHLDMVCQANTGTVHDFEHDPIDTILR
                     DGWLIAENTTLGADNGIGVALALAALEEPGLIHPPLEVLLTVNEESGMDGARGLAPGT
                     LQGRVLINLDTEEWGHFYLGCAGGVDVEVSHDCAPEHPFDITSDLPACGLSRMASSSI
                     KLRTDVLADYALIRLEVSGLRGGHSGIDIHEGRGNAIKLLAEALRDLSAHTDVRLIEM
                     AGGTARNAIPREAYALCALPVDMATNIDEWVRHRHQTWQARFAAADARVSFVCQHYDT
                     APDTAISHEERDRLLAFLDIAANGVAQVSDDFPGVTDTSSNLGVLTLRRGAFSATFKV
                     RSLHDARADALADKMVAYAAAQGLRAWKDGAYPGWTPNPESGLLALCQRVYTAEFGQA
                     ASLQVIHAGLECGLLAASHPHLDMISFGPDIRGAHAPGERVEIESVDRCWQLLKAILR
                     ALAKA"
     gene            5630..6781
                     /locus_tag="VFW59_12250"
     CDS             5630..6781
                     /locus_tag="VFW59_12250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5365026.1"
                     /translation="MNLDPRTLLFSLILVNAIMVLSLFVAASGNGRREGVGKWAVAIL
                     LETLTWILVAGRGVIPDLFSVIVANGLKAGAHAMILVAVYEFQRRTLPRWQWFVPVVL
                     TLLMSALLLDDIRGRFVWGGLIYGGQMALIAYALLSAPETRSARASKLLLGGIAMIVL
                     VLALRAFVALSGQSDLAQPQHAGSPHPVQILSFIAILATSLLGSIGFLLMVKERADKE
                     ILHLAMTDSLTGVPNRRSLMDQAERALARRSGKPLALFMIDVDHFKLINDTHGHPAGD
                     EVLRQISRLLEERLRGGDVLGRYGGEEFCVIAPDTDTAGALMLAESLRATIAATPLIT
                     GSGELFVTVSIGISHCPPDARRELKDVLADADAALYNAKQTGRNKVASR"
     gene            6783..8087
                     /locus_tag="VFW59_12255"
     CDS             6783..8087
                     /locus_tag="VFW59_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5365027.1"
                     /translation="MLNTAQTRQLVRLRWTAFVIVGLAYVLSFFHRFAPAAISGELQQ
                     TFHASASELGGLAATYFYVYTLMQIPTGILADTLGPRRVVAIGGLFAGIGSLLFGMAD
                     TLAAASAGRLLVGLGVSVTFISLLKLNAAWFHDRHFATMTGATILLGNVGSLLAATPL
                     AWALSFISWRTAFVAVGAFSLLLGALAWWLVRDHPGTVKLPSLRELDGKAAHPARSGH
                     WYDGLMTVLKNRATWPGLWVNMGIAGSMFAFAGLWAIPFLRDVYGMDRAAATSHTTTL
                     LAGFAIGAFFIGSLSDRLGRRKPVMAAGALAYSLCWLPLLFGMGMDNAASHALFFAMG
                     LAAPSFTLSWACAKEVNPHALSGMATSVVNIGAFLGTAIMQPLVGWAIDRAHAAHGDS
                     PLGPSDYRVGIAILLGFSAAGALATLFIRETYCRYGDTGDVD"
     gene            complement(8151..8792)
                     /locus_tag="VFW59_12260"
     CDS             complement(8151..8792)
                     /locus_tag="VFW59_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5365028.1"
                     /translation="MNDTSNSFLHELNDGELDILLRNGQKKFYAKNKLIIDDGDHSSS
                     AYIINSGKVKVFLSDDQGKEIVLCFLEAGEYFGEMALIDKEERSAAVMTMEDTELTEI
                     SQASFRECLKAHPELAERIMLGLVTRLREANKKISSLALEDVHERVANMLIDLAVKHN
                     GQLVIDDKPTHQHIANIVGASREMITRILKNMSADGHIEITGKQIIILNGQLR"
     gene            9221..9514
                     /locus_tag="VFW59_12265"
     CDS             9221..9514
                     /locus_tag="VFW59_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX5365029.1"
                     /translation="MEIPKRDGDGYLIDMNDWTEEVGRLMAEADGYELNEKKWGQIMK
                     AREYFEEFGSVPPIRKFAKYIEEDQKDVFELWMTGPMKPITKYGGMPKPTGCV"
     gene            complement(9555..13061)
                     /gene="dnaE"
                     /locus_tag="VFW59_12270"
     CDS             complement(9555..13061)
                     /gene="dnaE"
                     /locus_tag="VFW59_12270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5365030.1"
                     /translation="MPQPAFVHLRLHSEYSISDGMLRVSEAVSAAKSDAMPALALTDL
                     NNVFGLIKFYKAARGAGIKPVVGCDVFISNELDRDRPHRLLLLCQSREGYLLLCQLLS
                     RAFLENQYRGRAELRREWFHAGTGGLIALSGAHLGDVGTALLSGNATLARQCAEEWAS
                     LFANRYYIEIQRAGFNDDELHIHAATRLAAELALPVVATHPVQFLDASDFKAHEARVC
                     ISEGYVLNDKRRIKHFTDQNYFKTQAEMAQLFADLPEALANSVEIARRCNLSLTLGKP
                     RLPDFPTPNGESLDDFLRSESERGLMQRMALLYPDEAERAAKLPQYRERLEFETNTII
                     QMGFPGYFLIVADFIRWAKAYRSEDFPNGVPVGPGRGSGAGSLVAYCLLITDLDPLRY
                     ELLFERFLNPERVSMPDFDVDFCQDGRELVINYVKQQYGAQCVSQIATFGTMASKAVI
                     RDVGRVMDFPYGLCDSLSKAIPVEGVKPVSLAKAREMEPEINAIAEREEGVPELLELA
                     ERLEDLTRNVGMHAGGVLIAPGQLTDFCPLYCADSEASVVSQFDKDDVEAIGLVKFDF
                     LGLRTLTIIDWAMRYVARLNGGTAPFALETMPLDDKKSYDLLKKCNTTAVFQLESRGM
                     KDLIKRLQPDTFEDIVALVALFRPGPLESGMVEDFINRKHGKAVADYFHPDLEVSLKP
                     TYGVIVYQEQVMQISQIIGGYTLGGADMLRRAMGKKNPEEMAKHRDIFVDGAIKRGID
                     DKLATRLFDLMEMFAGYGFNKSHSAAYALVAYQTAYLKAHHPAAFMSATMSADLDNTE
                     KVKTFYQDTLQQGLQVLLPDVNSSGYRFSPVDEKTIAYGLGAVKGTGQAAIDNITEAR
                     KNGPFKDLFDFCRRVDKRVVNRRTIEALIKAGAFDAISDHRACMMATLDAALASADQQ
                     SRAANQNSLFGDDEADAVLTEQYADVVRWTLREQLAQEKISLGFYLGGHPYQEHEQEL
                     ANFIKVKLADLTPAFVGKSNGGGYGGGQGRPRGVAVTLAGIVSGVRLMQTRRGRMAVI
                     TLDDGAAQVEMTVFNEEYERSKPWIKEDEVLVVQGRASLDEYSGNVRVSGEELFDFAS
                     ARSHYAQQLALRCDGKVGIDRLKKIFTPYRDGKCQVMIHYRNHNSTCQLRLGEAWRVT
                     LDENLLRELREALQPDNVKVIY"
     gene            13221..13997
                     /locus_tag="VFW59_12275"
     CDS             13221..13997
                     /locus_tag="VFW59_12275"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX5365031.1"
                     /translation="MLRELRLFLTAVQFFTRIPVPAWVGHSAQQLAEAARYLPLVGVL
                     VGALTAATLWLAAQVLPLSLAVGLSMAAGILITGAFHEDGLSDFADGFGGGHTKEKML
                     AIMKDSRVGAYGVIAIALVLLLKYQALLELCRMHTPQFAIAALIAAHAVSRLMAASIM
                     LTQRYAREDDSTRAKPAAQLSPASFAIALVTGAAALGLLILSSPQATPVITAVSAALL
                     MRVYIGWRLQKRLGGYTGDCLGAVQQLSELAFYLGLLVVI"
     gene            13997..14536
                     /gene="cobC"
                     /locus_tag="VFW59_12280"
     CDS             13997..14536
                     /gene="cobC"
                     /locus_tag="VFW59_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HEX5365032.1"
                     /translation="MQTLYLIRHTKPRIAPGICYGQLDIEVAESFAEEANEVLNWLPP
                     VDLVFTSPLLRARRLADHLARHCELRSDARLMEKHFGAWEGKSWDDIPRQEIDAWAAD
                     VMGYAPDGGESAQQLMQRAQALLHDIAQLPQRQIALVAHGGSIRALLALAANIPLTKT
                     LNWQIDYGAVIALRTQHRP"
     gene            14602..14931
                     /locus_tag="VFW59_12285"
     CDS             14602..14931
                     /locus_tag="VFW59_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5365033.1"
                     /translation="MFTIQEIDAPEFAQWVNDANHQLRVIDVRQMEEIAMGTVPKAEA
                     VPLSTLPARLHELSPTEKLVIVCRSGARSGQACLFLQQQGFSNVYNLRGGMMRWVQSG
                     FPACQLG"
     gene            14955..15344
                     /locus_tag="VFW59_12290"
     CDS             14955..15344
                     /locus_tag="VFW59_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5365034.1"
                     /translation="MKSNKLFWALALALAVMFGMQQLQAADGVDVKQASSMSKQGALL
                     LDVREQEEYAAGHAPNAQLIPLGQLGARLPEIAAYKDKPIVVMCRSGRRSAKAVALLQ
                     EAGYSQVSNVKGGIQAWEGEGLEVVKM"
BASE COUNT         2844 a         5056 c         4666 g         2861 t
ORIGIN      
        1 gaattacatc aacaagacgc aagggcgtta ttgagtcgcg caccgatgag gggcatcgcc
       61 gcagcacccg agcgattgcc tcgccccctg taacggtgta ccttgccgca tcgtttctct
      121 gccggtttcc cctctgagcg ccatgctgga tttcaccgaa tacaccaaga tcttcatcag
      181 cctgttcgcg atcctcgatc cgatcgggat catccccatc atcgtgctgt tcaccgcaga
      241 gatgacgtca agcaagcgag cccaggtcgg acgcctcgcg tcgctgacgg tgtgcgcgat
      301 cctgctggcg gccctgctga tcggccagcc gctgctggcg ttcttcggca tcagcatcaa
      361 ttcgttccgc gtcgcgggcg gcatcctgtt gctgctgatg gcgatcaaga tgctgatggg
      421 caacttctac cgctccggca aggatgatca agtcggcgac gagacgcttt cggcgaatgc
      481 gatcgtgcct ttgtccacgc cgttgctggc gggccccggc tcgatcagcg cggtgatcgt
      541 cgaggcgcac aaggcggacg gcctgcagca ttatctgatc atgggtctgg agatcgtgct
      601 gctcagcatc accgtgtggc tgaccttcct gatcgcgccc tgggtcgcgc agcggatggg
      661 caagctcggc atcaacgtgt tcacccgctt gatgggcctg atactcgcgg cgatctccgt
      721 ggagttcatc gccagcggca tgcgcggcct gttcccggtg ctcggctaag cctgcaccga
      781 gggctgacca agacagggcg gagcaaaagc ccggcatagc agccggttcc cgattgtgcc
      841 gtatcatgtc gcccttccat cgttctgcaa cgccacaagc accctatgac cgaaaccttc
      901 atccccagca aagacgcttc cctcgaatcc tccatccgca cgctgcatgc caagctggaa
      961 gccatcggct ttcatgtcga ggagcgctcc tggctgaacg agatcgagaa catctggtcg
     1021 gtgcatgtca gcgaccgcga ttgcccgcgc ctgtttagca acggcaaggg cggctcggaa
     1081 ctggcggcgc gcgcgagcgc gctgggcgaa ttcttcgagc gtctgggcac caattatttc
     1141 tggacgcatt tctatctggg cgagaccctc agtcacgccg acttcgtgca ttacccgaac
     1201 gagcaatggt tcccggtgca gggcgacaag tggcccgccg cattgctgac gcccgagctg
     1261 cagaagttct acaacccgca aggcggcgtg ctggcgaaac agctgatcga cctgaattcc
     1321 ggcaatgccg agcgcggcat ctgcgcgatc ccctaccagc gtctgcgcga cgacaagacc
     1381 gtgctgttcc cggtcaacct gatcggcaac ctgtatgtca gcaacggcat gtcggcgggc
     1441 aacacgctga tggaggcgcg cactcaggcg ctggccgaaa tcttcgaacg cgacatcaag
     1501 ttccgcatca tccgcgaagg cctctgcctg cccgacgtgc ccgagacagt catcgcgcgc
     1561 tacccgcgca tcgccgcagg catcaagggc ctgcgcgagg cgggctacgg cattctggtg
     1621 aaggacgcaa gcttgggcgg ccagtatccg gtgatgaacg tcacgctgct gcacccggcc
     1681 gatcagggtt gcttcgcgag cttcggtgcg catccgcgct tcgaggtggc gctggaacgc
     1741 gcgctgaccg agctgctgca gggccgcgcc atcgacggcc tgaaggactt ccccgcgccc
     1801 ggcttcgaca tggaggagat cgccgacgag cagaatctgg agattcactt cgtcgattcc
     1861 agcggcgtga tcagctggga gtttttgcgc gacacgcccg acttcgaatt ctccgactgg
     1921 aacttcggca ccaccaccga ggaggattac cgctggtgct gcgatgcgat ccacgccagc
     1981 ggccacgaca tctatgtcgc cgacttcacc catctggacg tctatacctg ccgcatcttc
     2041 gtgcccggca tgtcggaaat ctacccgatc gaggaactgc aatggcagaa caacaccgtg
     2101 ggcaatctgg tgcgccccgc gctgctgcgc ctgccggaac tcgacgagga agaatgcaat
     2161 gacctgctcg acaccctgct cgatctcgat ctggcagaca acctcgcggt caccacgctg
     2221 atcggtctgg cggccgatcc gggcagcgtc tggatggatc tgcgcgtagg cgaactgaag
     2281 gccctgctgg cactcgccgt cggcgacgaa gacgccatcc tcgaaggctg cgactggata
     2341 gcccagctgg ggcagctgga agacaagcgt gcgcgcgtct accgttgcat ctcgcagctg
     2401 ctgcagctac aggagatggc ggactcgacg gctccctacg aagccagtct gcaactgctg
     2461 ttcggcgctg acaccctgca actggctcag cagctgctga gccgcgagac gcgattcttc
     2521 ggtctgggca cgctgggtgc gaacatggaa ggcagcaaca tgcatacccg cctgctagag
     2581 gcctatcgca aggtgtggaa acgctgattc caccgaccaa gccgaaagca ttgacgaccg
     2641 gagctgactg aaccgccccg gcagcggcgg ctcagtaacg cgagacgcga ccgctctcga
     2701 tacgcacccg gtctccgatg cgcaggtcat cgatgctctg ctgcgtgacg gtctgctccc
     2761 cgccgttctc cagccgcacc ctgacacgat aggaatctgc ctgccggttg ctcttctcga
     2821 tctggtgtcc caccacggca ccacccacga ctccggccac ggtcgcaaca tcgttgcccg
     2881 tgccaccgcc gacctgatgt ccgagcacgc cgcccaccac gccgccgatg accgtccctg
     2941 caccgatccc gccgtcactc ccccgcgtgg cctcgatgcc gtcgaccacg ccataggcca
     3001 gcgtcggaga aggcgagtgc gaatcggtcg tcgcgcaacc gcccattagc acggctgaca
     3061 agacaagaat cgcaccgaaa attctggatg ttttcatact ggcctcctgt tttctttgag
     3121 ccatcagtct gtacacatac ccgatcagcc tctgtgctgt aacgcactta agccgcgagg
     3181 atggcgacgg catgctgcgc ccctccgcag cagactgttc gcttgaagtg tctgggtgcc
     3241 ttcgcgcaca gacaacatcc cgcagatggg tgtaatctgc cctccacgta ttccaccagc
     3301 tctccgacaa gggagccgga tcatgcgcta tcaccgggag gcatcatgcg gggttggctg
     3361 aacaaactgt gttgcctgtc tctcattatc agcggaccgg ccgctgccgc accgggcaat
     3421 ttctccagcg tgatcggctc ggcactggtc tgcaacgatc aggtgaattc agcttatttc
     3481 aacggttata tgaagcagtt cttcggtgcg ccagcgttca cggcgggggg ggccaactgg
     3541 tggaaagtga acgacagcct cttcaacgcg cccgttgagt atgtattcgt cggaacaggc
     3601 cacgacttca tcggcgccac cttcagaacc cgccctgccg aattggttgt caatatcagg
     3661 gacgccatgg ggatcgaata caagcagatc agggccgaga cgtgggtttc cccggccgct
     3721 ggcgcgctga tcacctatca cgacaaaaac acccccagca agatgtactg cataggctcg
     3781 ccccacaccg cctattaggc tgttcgcagc gcgcacttca cccaatcgct atccggcctg
     3841 ccgcgttaca atctcgcgcc ccggcgggtc acatgaccgc cgcacccggg acgcgctgct
     3901 ggccgggttg ccgcaacggc gtcggtccgt tcggggctga gattattttt tggtatccct
     3961 cgatgacaca caattttgac gatctgcaac cgcgcccggt ctgggcccac ttcgccacct
     4021 tatgcgcgat accgcgcccc tcgcatcacg aggccgcgct gatagcgcat ctgatcggct
     4081 gggcgcaggc gcgcggcatc agcgccgggg tcgatggcgc cggcaacctg atcctgaaga
     4141 agcccgccag ccccggctgc gagtcgatgc ccggcgtgat cctgcaaggc catctcgaca
     4201 tggtgtgtca ggccaacacc ggcaccgtcc acgacttcga gcacgacccg atagacacca
     4261 tcctgcgcga cggctggctg atcgcagaga acaccacgct gggcgcggac aacggcatcg
     4321 gcgtcgcgct cgcattggcc gcgctggaag aacccggcct gatccacccg ccgctggagg
     4381 tgctgctgac cgtcaacgag gaatccggca tggacggtgc gcgcggtctc gcgcccggca
     4441 ccttgcaggg ccgggtgctg atcaatctgg ataccgagga atgggggcat ttctatctgg
     4501 gctgcgcggg cggcgtcgac gtggaggtaa gccacgattg cgcaccggaa catccattcg
     4561 acataaccag cgacttgccc gcttgcgggc ttagtcgaat ggctagtagt tccatcaagc
     4621 tcaggacaga cgtgctggcg gactacgcgc tgatacgcct ggaagtaagc gggctgcgcg
     4681 gcggccactc cggcattgac atccacgagg ggcgcggcaa tgcgatcaag ctgctggcag
     4741 aggcgttgcg cgacctgtcc gcgcacaccg atgtgcgcct gatcgagatg gcaggcggca
     4801 ccgcacgcaa cgcgatcccg cgcgaggcct atgccctgtg tgcgctgccg gtcgacatgg
     4861 cgacgaacat cgacgagtgg gttcggcacc ggcatcagac atggcaggcg cgattcgcgg
     4921 ctgccgatgc cagagtgtcg ttcgtctgcc agcattacga caccgcaccg gacaccgcca
     4981 tttcgcacga ggaacgcgac cggctgctcg ccttcctcga tatcgccgcg aatggcgttg
     5041 cacaggtcag cgacgacttc cccggcgtga ccgacacctc gagcaatctc ggcgtgctca
     5101 cgctgcggcg gggcgcgttc agcgcgacgt tcaaggtgcg ttcgctgcat gacgcacgcg
     5161 ccgatgcgct ggccgacaag atggtcgcct atgccgccgc ccaaggttta cgggcatgga
     5221 aggacggcgc ctatccgggc tggacgccga acccggaatc cggcctgctc gcgctgtgcc
     5281 agcgggtata caccgcagag ttcggacagg ccgccagcct gcaggtgatc cacgccggac
     5341 tggaatgcgg cctgctcgcc gccagccatc cgcatctgga catgatctcc ttcggccccg
     5401 acatccgagg tgctcatgcg ccaggcgaac gggtcgagat cgaatcggtc gatcgctgct
     5461 ggcagttgct gaaggcgata ttgcgggcgc tggccaaggc ctgatcgcac cgtttcggct
     5521 tcggatcggc cggcgaagaa ggtatagtgt ggggccgcat cagcagcgcc cgggctgttt
     5581 accgcctcca tcgaacgagc aaaacgcacc agaccatacc cgcgacacca tgaatcttga
     5641 cccccgcacc ttgctgttct cgctcattct ggttaacgcc atcatggtgc tcagcctgtt
     5701 tgtcgccgcc agcggcaatg gcagacgcga gggtgtcggc aaatgggcgg tcgcgatact
     5761 gctggagacc ctgacctgga tactggtcgc cgggcgcggc gtcatcccgg acctgttttc
     5821 ggtcatcgtc gccaatggcc tcaaggcagg cgcgcacgcg atgatactgg tggccgttta
     5881 tgagtttcag cggcgcacgc tgccgcgctg gcagtggttc gtgcctgtcg tattgacgct
     5941 gctgatgagc gcccttctgc tggacgacat acgcggccgc ttcgtctggg gaggcctgat
     6001 ctatggcgga cagatggcgc tgatcgccta cgcgctgttg tcagcccccg agacccgctc
     6061 cgcacgcgcc tcaaagctgt tgctcggcgg catcgcgatg atcgtgctgg tgctcgcgct
     6121 gcgagccttt gtcgccctgt ccggccagtc cgatctggct cagccccagc atgccggctc
     6181 acctcatccg gtgcagatcc tctccttcat cgccatcctg gcgacttcgc tgctgggttc
     6241 gatcggtttc ctgctgatgg tcaaggaacg cgcggacaag gagatcctgc atctggcgat
     6301 gacagacagc ctcaccggcg tgcccaaccg gcgctcgctg atggatcagg ccgaacgcgc
     6361 gctggcccgg cgcagcggca aaccgctggc gctattcatg atcgacgtcg atcacttcaa
     6421 actgatcaat gacacgcacg gccatccggc gggcgacgag gtgctgcgcc agatcagccg
     6481 cctgctggaa gagcggctgc gcggcggcga cgtgctggga cgctacggcg gggaagagtt
     6541 ctgcgtgatc gcgccggaca cagatactgc gggcgcgctg atgctggcag agtccctgcg
     6601 cgcgacgata gcagccacgc cgctcatcac cggcagcggc gaactcttcg tcacggtcag
     6661 catcggcatc tcgcactgcc cgcccgatgc ccggcgtgaa ctgaaagatg ttctggccga
     6721 tgccgatgcc gcgctctaca acgcaaaaca aaccgggcgc aacaaagtag ccagccgctg
     6781 aaatgctgaa caccgcccaa acccgccaac tcgtgcgcct gcgctggact gcattcgtca
     6841 tcgtcgggct ggcctatgtg ctgtcgttct tccaccgttt cgccccggcg gcgatctccg
     6901 gcgagttgca gcagactttc catgccagcg catccgaact gggcggattg gcggcgacct
     6961 atttctacgt ctatacgctg atgcagattc ccaccggcat cctcgccgat acgctggggc
     7021 cgcgccgggt ggtggcgatc ggcggcctgt tcgcgggtat cggctcgctg ctgttcggca
     7081 tggccgatac gctggctgcg gcatcggccg gccgcctgct ggtgggtctc ggcgtgtcgg
     7141 tcaccttcat ctcgctgctc aagctcaacg cagcctggtt ccacgaccgg catttcgcca
     7201 cgatgaccgg cgcgaccatc ctgctcggca atgtcggctc gctgctggcg gcgacgccgc
     7261 tggcctgggc gctgagcttc atctcctggc gcaccgcctt cgtcgcggtg ggcgctttct
     7321 cgctgctgct gggtgcgctt gcctggtggc tggtgcgcga ccatccgggt acggtgaagc
     7381 tgcccagcct gcgcgagctc gatggcaagg cggcgcaccc ggcgcgcagc ggacattggt
     7441 acgacgggct gatgaccgtg ctgaagaacc gcgccacctg gccggggctg tgggtgaaca
     7501 tggggatcgc aggttcgatg ttcgcctttg ccggcttgtg ggccatcccc tttctgcgcg
     7561 acgtgtacgg catggacagg gcggccgcca ccagccacac cacgacgttg ctcgccggtt
     7621 ttgctatcgg cgcattcttc atcggctcgc tgtcggaccg gctcggacgg cgcaagccgg
     7681 tgatggcggc gggtgcgctg gcatacagtc tgtgctggct gccgctgctg ttcggcatgg
     7741 gcatggacaa tgcggccagc catgcgctgt tcttcgcgat ggggctggcc gcacccagct
     7801 tcacgctgag ctgggcctgc gccaaggagg tgaatccgca tgcgctgtcg ggcatggcaa
     7861 ccagcgtggt gaacatcggc gccttcctcg gcaccgccat catgcaaccg ctggtgggct
     7921 gggcgatcga ccgcgcccat gccgctcacg gcgattcgcc gctcggcccg agcgactatc
     7981 gggtcggcat cgcgatcctg ctgggatttt cggctgcggg agcgctcgcg acactgttca
     8041 tccgcgagac ctattgccgc tatggggata ccggggatgt cgattgactg ccggtgcgca
     8101 gcccgccccg aatggagcag actgccgcag caatgaaccg acggcgagtc ttatcgtaac
     8161 tgcccgttca ggatgatgat ctgctttccg gtgatttcga tatgtccgtc agcagacatg
     8221 ttcttcagga tgcgcgtaat catttcgcga gaagcgccga cgatgttggc aatatgctga
     8281 tgggtcggtt tgtcatcgat caccagttga ccgttatgct tcacggccag atcgatcagc
     8341 atattcgcca cccgctcatg cacatcctcc agcgccagac tggaaatctt cttgttcgcc
     8401 tcgcgcagac gcgtcaccag ccccagcatg atgcgctcgg ccagttccgg atgggctttc
     8461 aggcactctc tgaaactcgc ctgactgatc tcggtcaact ccgtgtcttc catggtcatg
     8521 accgcagcag agcgttcttc cttgtcgatc agagccatct cgccaaagta ttcgcccgcc
     8581 tccaggaagc acaggacgat ctccttgccc tgatcgtcgc tgaggaaaac cttcaccttg
     8641 ccggaattga tgatgtaggc ggaacttgaa tggtctccgt cgtcgatgat gagtttgttt
     8701 ttcgcgtaga acttcttctg cccattgcgc agcagaatgt ccagttcacc gtcattcagc
     8761 tcatgtagaa aagagttcga tgtatcgttc ataggtattg aagtgggtcc agcctcgaaa
     8821 aaattaaaca acatcaggca aggccatcgt gccgcctgcc aacaccccgc atgaaggaac
     8881 tccccggccc gtcttacggc ggccaagaat actccgtcaa caagggcatg tgaatagtga
     8941 catacccgcc cctgacagca gaaggaactt tgtcacaaca ccacccgcct ctggccatca
     9001 ttgagccgtt ttgttcccca tctctgcgaa tgcagtgaaa ttatgcatcg cacccttcgc
     9061 ccgaccggga aacatccccc cgccgatcat cttgcgtccc gaacatgaca gcttcaagtc
     9121 acccgcccgg cctttccgta tcagccgcac aaaatacttg accaaaatgc ttaggaacag
     9181 ttaaatgacc ctatagtctc tccaacaaaa ggtgctgcac atggaaatcc cgaaacgcga
     9241 tggcgatggc tatctgatcg acatgaacga ctggactgaa gaggtcggcc gcttgatggc
     9301 cgaggcggat ggctacgaat tgaacgaaaa gaagtggggt cagatcatga aggcccgcga
     9361 atatttcgag gaattcggct cggtaccgcc gatccgcaaa ttcgccaagt acatcgagga
     9421 agaccagaag gatgtgttcg aactgtggat gaccggcccg atgaagccca tcaccaaata
     9481 cggcggcatg cccaagccta ccggctgcgt ctgagaacaa cctcccgggc cgccctctgg
     9541 cgccccccta tgcgtcaata gatcaccttg acgttgtccg gctgcaacgc ctcgcgcaat
     9601 tcgcgcaaca ggttctcgtc cagcgtcacc cgccatgcct cgcccagacg caattgacag
     9661 gtcgagttgt ggttgcggta atggatcatc acctggcatt tgccgtcgcg atagggagtg
     9721 aaaatctttt tcagccggtc gatgccgacc ttgccgtcgc aacgcaaggc cagttgctgg
     9781 gcgtagtggg aacgcgccga ggcgaaatcg aatagctctt cgccgctgac ccgcacgttg
     9841 ccggagtact cgtccagact ggcgcgcccc tgcaccacca gcacttcgtc ttccttgatc
     9901 cagggtttgc tgcgctcgta ttcctcgttg aacaccgtca tctcgacctg agccgcaccg
     9961 tcatcgagcg tgatcacggc catgcgcccg cgccgcgtct gcatcaggcg cacgcccgac
    10021 acgatgcccg ccagcgtcac cgcgactccg cgcggcctgc cctgccctcc gccgtagccg
    10081 ccgccattgg acttgccgac gaatgcaggc gtaaggtcgg cgagcttcac cttgatgaag
    10141 ttggccagct cctgctcatg ctcctgatag ggatggccgc cgaggtagaa acccagactg
    10201 atcttttcct gcgccagctg ctcgcgcaag gtccagcgca ccacatcggc gtattgctcc
    10261 gtcagcaccg catcggcctc gtcgtcgccg aacaggctgt tctggttcgc cgcgcgcgac
    10321 tgctgatcgg cgctcgccag cgccgcatcc agcgtcgcca tcatgcaggc gcggtgatcg
    10381 ctgatcgcat cgaatgcgcc cgccttgatc agcgcctcga tggtgcggcg gttgaccacg
    10441 cgcttgtcga cgcggcggca gaagtcgaac agatccttga acggcccgtt cttgcgcgcc
    10501 tcggtgatat tgtcgatcgc agcctggccg gtgcctttga ccgcacccag accataggcg
    10561 atggtctttt cgtccaccgg cgagaagcga tagcccgaac tgttcacgtc cggcaacagc
    10621 acctgcaaac cctgctgcaa ggtgtcctga tagaaggtct tgaccttctc ggtgttgtcc
    10681 agatcggccg acatcgtcgc ggacatgaac gccgccgggt gatgggcctt gaggtaggcg
    10741 gtctggtagg ccaccagcgc ataggcggcc gagtgcgact tgttgaagcc gtaacccgcg
    10801 aacatctcca tcaggtcgaa caaacgcgtc gcgagtttgt cgtcgatgcc gcgcttgatc
    10861 gcgccgtcga cgaagatgtc gcgatgcttg gccatctcct ccggattctt cttgcccatc
    10921 gcgcggcgca gcatgtccgc gccgcccagc gtgtaaccgc cgatgatctg cgagatctgc
    10981 atcacctgct cctggtacac gatcacgccg taggtcggct tgagcgaaac ttccaggtca
    11041 ggatggaaat aatcggcgac ggccttgccg tgtttgcggt tgatgaagtc ctccaccatg
    11101 ccggattcca gcggtcccgg acggaacagc gcgaccagcg cgacgatgtc ctcgaaggtg
    11161 tcaggctgca gccgcttgat cagatccttc atgccgcgcg attccagctg gaacacggcg
    11221 gtggtgttgc atttcttcag caggtcgtag gacttcttgt cgtccagcgg catggtctcc
    11281 agcgcgaacg gtgcggtgcc gccattcagg cgcgcgacgt agcgcatcgc ccagtcgatg
    11341 atggtcagcg tgcgcagccc cagaaagtcg aacttcacca gaccgatggc ctcgacgtcg
    11401 tccttgtcga actggctgac cacgctggcc tcggagtcgg cgcaatacag cgggcagaag
    11461 tcggtgagct gtcccggcgc gatcagcacg ccgcccgcat gcatgccgac gttgcgcgtc
    11521 agatcttcca gacgttcggc cagttccagc agctcgggca cgccctcctc gcgctcggcg
    11581 atcgcgttga tctccggctc catctcgcgc gccttggcga gcgacaccgg cttgacccct
    11641 tcgaccggga tcgccttgga caggctgtcg cacaggccgt acgggaaatc catcacccgc
    11701 ccgacgtcgc ggatcaccgc cttggacgcc atggtgccga aggtcgcgat ctgcgacacg
    11761 cactgcgcgc cgtattgctg cttcacatag ttgatgacca gctcgcgccc gtcctgacag
    11821 aagtccacgt cgaagtcggg catcgagacc cgctcgggat tcaggaagcg ctcgaacagc
    11881 agctcgtagc gcagcgggtc gagatcggtg atcagcaggc agtaagccac cagcgagccc
    11941 gcaccggatc cccgcccggg accgaccggc acgccattgg ggaaatcctc ggaacgatag
    12001 gccttggccc agcggatgaa gtccgccacg atcaggaagt agcccgggaa gcccatctgg
    12061 atgatggtat tggtctcgaa ctccagccgt tccctgtact gaggcagctt cgcggcgcgc
    12121 tccgcttcgt ccggatagag cagcgccatg cgctgcatca ggccgcgctc ggattcgctg
    12181 cgcaggaagt cgtccagact ctcgccgttc ggcgtgggga agtccggcag gcgcggcttg
    12241 cccagcgtca gcgacagatt gcagcgccgc gcgatctcca cgctgttggc gagcgcctcg
    12301 ggcaggtcgg cgaacagctg ggccatctcg gcctgggtct tgaaataatt ctggtcggtg
    12361 aagtgtttga tgcggcgctt atcgttgagc acatagccct cggagatgca cacgcgcgcc
    12421 tcatgcgcct tgaagtcgga cgcatcgagg aactgcaccg gatgggtcgc gaccaccggc
    12481 agcgcaagct cggctgccag ccgggtcgcg gcatggatat gcaactcgtc gtcgttaaat
    12541 ccggcgcgct gtatctcgat gtaatagcgg ttggcgaaca gcgacgccca ttcctccgcg
    12601 cactgtctgg ccagggtggc attgccggac agcagcgcgg taccgacatc gcccagatgc
    12661 gcgccggaca gggcaatcag cccgcccgtg cccgcatgga accattcgcg ccgcaactcg
    12721 gcgcggccgc gatactggtt ctccagaaag gcgcgcgaca gcaactggca cagcagcaga
    12781 taaccctcgc gcgactgaca cagcagcagc agacgatgcg ggcggtcgcg gtcaagctcg
    12841 ttgctgatga acacatcgca gccgaccacc ggcttgatgc cagcgccgcg cgccgccttg
    12901 tagaacttga tcaggccgaa tacgttgttc aggtcggtca gcgccagcgc gggcatcgcg
    12961 tcgctcttcg cggcggatac cgcctcgctc acgcgcaaca tcccgtccga aatggaatac
    13021 tcgctgtgca gacggaggtg aacgaaggct ggttgaggca tgacgggaac gatcaaaaat
    13081 caggaacgga ttttagcatc gctggcgcat tccgtttccc ggatatcgca attgaattgc
    13141 agtcatcggc ccaaccttac ggctggacag gccgcgcacc cgctgctagt ctgcctgcgc
    13201 cttcatttcc cccgcagacc atgctacgag aactgcgctt attcctcacc gccgtccaat
    13261 tcttcacgcg catcccggtg cccgcatggg tcggccattc cgcacagcag ctggccgaag
    13321 cggcgcgcta cctcccgctg gtcggcgtac tggtgggggc gctgaccgcc gccacgctat
    13381 ggctcgcagc ccaggtgctg ccgctgtcgc tggccgtagg cctgagcatg gcggccggca
    13441 tcctgatcac cggcgcgttc cacgaggacg gcctctccga cttcgccgac ggtttcggcg
    13501 gcggccacac gaaagagaag atgctggcga tcatgaagga ctcgcgcgtc ggcgcttacg
    13561 gcgtgatcgc catcgcactg gtgctgctgc tgaaatatca ggcgctgctg gaactgtgcc
    13621 gcatgcacac gccgcaattc gccatcgccg cgctgatcgc cgcacacgcg gtaagccgcc
    13681 tgatggccgc cagcatcatg ctgacccagc gctatgcgcg cgaggatgac agcacccgcg
    13741 ccaaacccgc cgcccagctc agccccgcca gcttcgccat cgcgctggtc accggcgcgg
    13801 ccgcactcgg gctcctgatc ctatccagcc cgcaggccac cccggtcatc accgccgtat
    13861 ccgccgcgct gctgatgcgc gtctatatcg gatggcgctt gcagaaacgt ctgggcggct
    13921 acaccggaga ctgtctgggc gcggtgcagc aactgagcga actggcgttc tatctgggcc
    13981 tgctggtcgt catttaatgc agacgctgta cctgatccgc cataccaagc cgcgcatcgc
    14041 gcccggcatc tgctacggac agctagacat cgaggtggcc gaaagcttcg cagaggaggc
    14101 gaacgaggtg ctgaactggc tgccgccggt tgacctcgtc ttcacctcgc cgctgctgcg
    14161 tgcccgccgg ctggccgacc atctggcgcg gcactgcgag ctgcgcagcg acgcacgcct
    14221 gatggaaaaa cacttcggcg catgggaagg aaaatcatgg gacgacatcc cccgccagga
    14281 aatcgacgcc tgggctgccg atgtgatggg ctacgcgccc gacggcggcg aatcggcaca
    14341 gcagctgatg caacgcgcac aggctttgtt gcatgacatc gcgcaattgc cgcaacgcca
    14401 gatcgccctc gtcgctcatg gcggcagcat ccgcgcgctg ctggcgctgg ccgcgaacat
    14461 cccgctgacg aagacgctga actggcagat cgactatggt gcggtgatcg ccctgcgcac
    14521 acagcaccgc ccgtaaccgc tggcctgcct gaagattaga gtgtgctaat attcaaaaat
    14581 ctattcattc aggagcaagc catgttcacc atccaggaaa tcgacgcacc cgaattcgcc
    14641 cagtgggtga acgatgccaa ccaccagctg cgcgtgatcg acgtgcgcca gatggaagaa
    14701 atcgcaatgg gcaccgtgcc gaaggccgaa gccgtgccgc tgagcaccct gcccgccagg
    14761 ctgcacgaac tgtccccgac cgagaagctg gtgatcgtct gccgcagcgg cgcccgctcc
    14821 ggacaggcct gcctgttcct gcagcagcag ggattctcca acgtgtacaa cctgcgcggc
    14881 ggcatgatgc gctgggtgca gagcggcttc cccgcctgcc agctcggcta aactaacctc
    14941 accagggaca cgcaatgaaa tcgaacaaac tattctgggc gctggcccta gcgctggccg
    15001 tgatgttcgg catgcagcaa ctgcaagccg ccgatggcgt cgacgtcaaa caggcatcca
    15061 gcatgagcaa acagggcgcg ctgctgctgg acgtgcgcga gcaggaagaa tacgccgcag
    15121 gccacgcccc gaacgcgcag ctgatcccgc tgggccagct gggcgcgcgc ctgccggaaa
    15181 tcgccgccta caaggacaaa cccatcgtcg taatgtgccg ctccggccgc cgctcggcca
    15241 aggccgtcgc gctgctgcag gaagccggct acagccaggt cagcaacgtg aagggcggaa
    15301 tacaggcgtg ggaaggcgaa gggctggaag tggtgaagat gtagcggttg tatttctgga
    15361 gctaaaacca aaaccgccga tggcagcacc cgcaaggagt gcgccgccag acgtttgccc
    15421 tttctgg
//
